KATO Nobuhiro

Department of Biomedical EngineeringProfessor/Assistant Dean/Senior Staff

Last Updated :2024/09/22

■Researcher basic information

Degree

  • PhD（Osaka Prefecture University）

J-Global ID

Research Keyword

  • Microfluidics   Microneedles   photo-lithography   bio-MEMS   Atomic Force Microscope   

Research Field

  • Life sciences / Medical systems
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Measurement engineering
  • Informatics / Mechanics and mechatronics
  • Nanotechnology/Materials / Nano/micro-systems
  • Nanotechnology/Materials / Nanomaterials
  • Life sciences / Biomaterials
  • Life sciences / Biomedical engineering

■Career

Career

  • 2017/04 - Today  Kindai UniversityDepartment of Biology-Oriented Science and TechnologyProfessor
  • 2006/04 - 2017/03  Kindai UniversityDepartment of Biology-Oriented Science and TechnologyAssociate Professor
  • 2006/08 - 2007/07  University of MichiganDepartment of EngineeringVisiting Scholar
  • 2002/04 - 2006/03  Kindai UniversityDepartment of Biology-Oriented Science and TechnologyLecturer
  • 1998/04 - 2002/03  Kindai UniversityFaculty of Biology-Oriented Science and Technology助手
  • 2000/12 - 2001/03  大阪府地域結集型共同研究事業非常勤研究員(兼任)

■Research activity information

Award

  • 2024/06 精密工学会関西支部 ベストポスタープレゼンテーション賞
     Stanford A 型大動脈解離治療における組織保護のためのマイクロニードルメッシュシート 
    受賞者: 岡田瑶平;伊藤巧真;吉川奏楽;加藤暢宏;田中篤;本田賢太朗;西村好晴
  • 2023/03 精密工学会 2023年度春季大会 ベストプレゼンテーション賞
     烏賊の吸盤を⽣体模範したマイクロニードルメッシュシートの作製と評価 
    受賞者: 浅見泰雅;定野和憲;高谷亮太;本田賢太郎;西村好晴;田中 篤;加藤暢宏
  • 2022/11 Best Paper Award
     Biodegradable Mesh Sheets as a Treatment Device for Acute Type A Aortic Dissection Equipped with North American Porcupine-inspired Microneedle for Removal Prevention 
    受賞者: Taiga Asami;Shunsuke Imamura;Tomoya Akaki;Kentaro Honda;Yoshiharu Nishimura;Atsushi Tanaka;Nobuhiro Kato
  • 2019/11 The First Asian Pacific Division of the World Federation for Laser Dentistry Conference 2019 Award of Excellence Poster
     Repair of enamel pits using Er:YAG laser film deposition method 
    受賞者: Tomoki Minamino;Ei Yamamoto;Nobuhiro Kato;Hiroaki Nishikawa;Shigeki Hontsu
  • 2019/11 The First Asian Pacific Division of the World Federation for Laser Dentistry Conference 2019 Award of Excellence Poster
     Occulusion of dental tubule with amorphous calcium phosphate sheet prepared by KrF pulsed laser deposition 
    受賞者: Nobuhiro Kato, Mizuki Hirai, Tomoki Minamino, Kazushi Yoshikawa Shigeki Hontsu
  • 2019/09 精密工学会 ベストプレゼンテーション賞
     メッシュ状の基部をもつポリ乳酸製マイクロニードルパッチの⽌⾎能⼒の評価 
    受賞者: 千原なみえ;坂本佳奈子;横山真央;樽谷玲;田中篤;赤坂隆史;加藤暢宏
  • 2014/10 日本歯科保存学会 カボデンタル優秀ポスター賞
     Er:YAGレーザー成膜方による歯質上へのアパタイト膜の直接形成 
    受賞者: 本津 茂樹;山本 衛;加藤 暢宏;西川 博昭;吉川 一志;山本 一世
  • 2004 日本機械学会 日本機械学会 教育賞
     産学連携した３次元CAD設計教育 JPN 
    受賞者: 藤井 雅雄;加藤 暢宏;上田 和弘;清水 隆紀

Paper

  • 加藤 暢宏
    光技術コンタクト = Optical and electro-optical engineering contact 東久留米 : 光学工業技術協会 61 (2) 21 - 25 0913-7289 2023/02
  • Atsushi Tanaka; Akira Taruya; Kyosuke Shibata; Kota Fuse; Yosuke Katayama; Mao Yokoyama; Manabu Kashiwagi; Ota Shingo; Takashi Akasaka; Nobuhiro Kato
    Scientific reports 11 (1) 13 - 13 2021/01 [Refereed]
     
    Refractory angina is an independent predictor of adverse events in patients with vasospastic angina (VSA). The aim of this study was to investigate the relationship between coronary lumen complexity and refractory symptoms in patients with VSA. Seventeen patients with VSA underwent optical coherence tomography. The patients were divided into the refractory VSA group (n = 9) and the stable VSA group (n = 8). A shoreline development index was used to assess the coronary artery lumen complexity. Shear stress was estimated using a computational fluid dynamics model. No difference was observed in the baseline characteristics between the two groups. The refractory VSA group showed the higher shoreline development index (refractory VSA 1.042 [1.017-1.188] vs stable VSA 1.003 [1.006-1.025], p = 0.036), and higher maximum medial thickness (refractory VSA 184 ± 17 μm vs stable VSA 148 ± 31 μm, p = 0.017), and higher maximum shear stress (refractory VSA 14.5 [12.1-18.8] Pa vs stable VSA 5.6 [3.0-10.5] Pa, p = 0.003). The shoreline development index positively correlates with shear stress (R2 = 0.46, P = 0.004). Increased medial thickness of the coronary arteries provokes lumen complexity and high shear stress, which might cause refractory symptoms in patients with VSA. The shoreline index could serve as a marker for irritability of the medial layer of coronary arteries and symptoms.
  • Mao Yokoyama; Namie Chihara; Atsushi Tanaka; Yosuke Katayama; Akira Taruya; Yuko Ishida; Mitsuru Yuzaki; Kentaro Honda; Yoshiharu Nishimura; Toshikazu Kondo; Takashi Akasaka; Nobuhiro Kato
    Scientific reports 10 (1) 18831 - 18831 2020/11 [Refereed]
     
    Management of bleeding is critical for improving patient outcomes. While various hemostatic products are used in daily practice, technical improvement is still needed. To addresses this problem, we newly developed a microneedle hemostatic sheet based on microneedle technology. We demonstrated the unique features of this microneedle hemostatic sheet, including reduced hemostatic time, biodegradable polymer composition that allows intracorporeal use without increasing infectious risk incorporation of microneedles to fix the sheet to the wound even on the left ventricular wall of a swine while beating, and a mesh structure with flexibility comparable to that of bonding surgical tape and sufficient rigidity to penetrate human aorta tissue and swine left ventricular wall. One potential application of the microneedle hemostatic sheet is intracorporeal topical hemostasis for parenchymatous organs, large vessels, and heart wall during trauma or surgery, in addition to new, widespread applications.
  • Nobuhiro Kato; Mizuki Hirai; Ei Yamamoto; Shigeki Hontsu
    Key Engineering Materials 829 KEM 16 - 20 1013-9826 2020 [Refereed]
     
    © 2020 Trans Tech Publications Ltd, Switzerland. Dental materials purely composed of calcium phosphate are ideal for dental medicine. We have been developing ultrathin amorphous calcium phosphate (ACP) sheets for enamel repair. In this study, long term stability of the ultrathin ACP sheets applied on enamel surfaces which kept in a simulated intraoral condition for 21 days was evaluated. Surface morphologies of the ACP sheets were observed by optical images. A boundary condition between the ACP sheet and the enamel was evaluated by cross-sectional SEM image. Durability of the ACP sheet was evaluated by a brushing test conducted after 21 days incubation. As a result of these evaluations, it was confirmed that the ACP sheets were stable even after long-term incubation under the simulated intraoral environment condition.
  • Daisaku Iwamoto; Nobuhiro Kato; Shunji Taniguchi; Yoshitomo Taguchi; Masao Kishi; Kazuhiro Saeki
    International Journal of Biomaterials HINDAWI LTD 2018 7546986 - 7546986 1687-8787 2018 [Refereed]
     
    © 2018 Daisaku Iwamoto et al. Single embryo culture is useful for assessing the developmental competence of an embryo in detail. Recently, a device made of poly(dimethylsiloxane) (PDMS), which is biocompatible and nontoxic, has been widely used for culture various types of cells. However, PDMS plates are porous, causing the serious osmolality increment of the medium (over 600 mOsm/kg from Day 4 to Day 7). Here, we report that curing the PDMS under low pressure (LP-PDMS) greatly reduced the porosity, resulting in a constant osmolality of the medium. The blastocyst rate of single bovine embryos cultured with LP-PDMS microwell (MW) plates was the same as that of group-cultured embryos (25 embryos/50 μl droplet; control, P>0.05). These results indicate that MWs on a plate made of PDMS cured under low pressure can be successfully used for individual embryo culture.
  • Nobuhiro Kato; Yuki Ido; Ei Yamamoto; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 758 KEM 162 - 165 1013-9826 2017 [Refereed]
     
    © 2017 Trans Tech Publications, Switzerland. In cosmetic dentistry, bleaching and laminate veneer methods are common treatments to improve the appearance of teeth. However, there are limitations to these treatments, as they damage the enamel which is not capable of recovering naturally. In this study, novel hydroxyapatite (HAp) sheets, which enable both enamel restoration and aesthetic treatments, were developed. Results indicated that the lower range of the sintering temperatures, of the pulsed laser deposition targets, produced lighter shades of amorphous calcium phosphate (ACP) sheets. Subsequently, the post-Annealing process of the sheets induced crystallization processes of the ACP sheets, to form HAp sheets, and improved the shade rank of the sheet. The shade rank of the developed sheet (6.8 ± 0.2) was almost comparable to the average shade rank of the teeth of Japanese people, within a range of 6.5 to 7.0.
  • Ei Yamamoto; Nobuhiro Kato; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 758 KEM 97 - 104 1013-9826 2017 [Refereed]
     
    © 2017 Trans Tech Publications, Switzerland. Hydroxyapatite (HAp) is one of the main structural components in tooth. Therefore, the HAp is an ideal material to repair tooth substances. However, it has not yet been realized that tooth enamel and dentin are artificially repaired using the HAp in operative dentistry. We have proposed and developed a tooth restoration technique with intraoral laser ablation using an Er:YAG (Erbium: Yttrium-Aluminum-Garnet) laser in order to fabricate HAp films on tooth surfaces in the atmosphere at room temperature. Although HAp thin films were formed on dentin surfaces using the Er:YAG laser deposition method, it has still been unclear how is the mechanical durability of the HAp film on tooth substances. In the present study, we conducted brushing tests to evaluate the durability of adhesion between the HAp film and dentin. To form HAp layers on dentin surfaces, an ablation phenomenon was induced by the Er:YAG laser irradiated to the target of α-Tricalcium phosphate (α-TCP) under humid conditions. The deposited layers were hydrolyzed by dripping artificial saliva on the surface at 37 degrees Celsius for 24, 48, 72, 120, and 168 hours to create HAp films. The films hydrolyzed for more than 48 hours were attached on the dentin surface after the brushing tests. This result indicates that the α-TCP layer deposited on the dentin surface changes into a crystallized HAp material by the hydrolyzation for more than 48 hours. The crystallization of the film possibly contributes to the high adhesive durability of the HAp film deposited on the dentin.
  • Nobuhiro Kato; Yuka Hatoko; Ei Yamamoto; Tsutomu Furuzono; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 720 102 - 107 1013-9826 2017 [Refereed]
     
    © 2017 Trans Tech Publications. Potassium-substituted calcium phosphate free standing sheet for the treatment of dentin hypersensitivity was prepared and evaluated. The appropriate annealing condition for the target of pulsed laser deposition to prepare the potassium-substituted amorphous calcium phosphate (PS-ACP) sheet was investigated. The target annealed at 1000 °C was used to deposit the PS-ACP sheet. The annealing condition for the potassium-substituted hydroxyapatite (PS-HAp) sheet was evaluated by XRD and EDX. The potassium release rate from PS-ACP sheet in PBS, evaluated by ICP-MS, was 2.5 times larger than that of PS-HAp sheet. The robustness of the PS-ACP sheet was proved by the brushing test of the PS-ACP sheet affixed on a bovine dentin. The dentin tubules on the dentin surface was occluded by the PS-ACP sheet and which confirmed by SEM observations.
  • Ei Yamamoto; Nobuhiro Kato; Yuka Hatoko; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 720 269 - 274 1013-9826 2017 [Refereed]
     
    © 2017 Trans Tech Publications. Restoration and maintenance of tooth substance are primary concerns in operative dentistry. Intact tooth is mainly composed of hydroxyapatite (HAp). However, there are no practical HAp materials for the treatment of tooth decay and hypersensitivity. We have newly proposed and developed a tooth restoration technique with intraoral laser ablation using an Er:YAG laser in order to form the HAp thin film on tooth surface in the atmosphere at room temperature. However, the fabricated HAp film is not uniform for the present due to the unsettled water condition at the material surface during the laser ablation. In the present study, we tried to optimize the humid condition using a nebulizer for the fabrication of HAp layer with the Er:YAG laser deposition method. The HAp layers were fabricated on a titanium substrate while changing the flow rate of water mist in order to determine the relationship between the spraying amount and the HAp layer structure. It is revealed that there exists the optimal humid condition to fabricate the uniform hydroxyapatite coating film with the Er:YAG laser deposition method.
  • Hontsu Shigeki; Hirai Mizuki; Katayama Ichiro; Yamamoto Ei; Kato Mobuhiro; Yoshikawa Kazushi
    Journal of Bio-Integration Bio Integration Society 7 (1) 39 - 43 2186-2923 2017 [Refereed]
     
    Tooth bleaching and laminate veneer restoration methods are widely used in cosmetic dentistry. However,despite their aesthetic appeal, these methods damage tooth enamel, which cannot regenerate, and the adhesiveused with laminate veneers can cause an allergic reaction. We are attempting to develop a novel esthetic dentistrysheet that would be applicable to both enamel restoration and cosmetic dentistry. An amorphous calciumphosphate (ACP) sheet was fabricated from low density hydroxyapatite (HAp) using a pulsed laser depositionmethod. The whiteness level of the sheet increased, as judged from the increase in the number of particles onthe sheet surface. The HAp sheet was crystallized using a post-annealing process, and this also increased thewhiteness of the sheet compared to an ACP sheet for the growth of a grain boundary introduced into the sheetby crystallization. A change in whiteness of the opaque white sheets according to the Commission Internationalede l'Eclairage (WICIE) masked the discoloration of a human avulsed tooth, as measured using a spectrum colorimeter.The whiteness values increased and the yellow values decreased as the number of overlapping sheetsincreased, demonstrating that the aesthetic effects of opaque white HAp sheets are very promising in cosmeticdentistry.
  • Takahisa Kai; Shunta Mori; Nobuhiro Kato
    ADVANCED BIOMEDICAL ENGINEERING JAPANESE SOC MEDICAL & BIOLOGICAL ENGINEERING 5 (5) 63 - 67 2187-5219 2016 [Refereed]
     
    Moving-mask lithography with backside exposure was utilized to generate master male mold for biodegradable polymer microneedle production. The microneedle shape was calculated from the exposure dose, mask geometry, and moving trajectory using a newly developed computer simulation. Two conditions (90 mu m aperture with 80 mu m diameter of circular movement, and 90 mu m aperture with 90 mu m diameter of circular movement) were selected to evaluate the moving-mask exposure effectiveness. By changing the moving trajectory, two different sizes of microneedles were obtained from a single-size aperture mask. The fabricated microneedle and calculated microneedle geometry showed good qualitative agreement. The geometrical difference was 2% in basal diameter and 8%-16% in height. Using the master male mold, biodegradable polymer microneedles made of chondroitin sulfate C sodium salt (CSC) were fabricated by casting from a poly-dimethylsiloxane female mold. The shape of the biodegradable CSC microneedles showed good agreement with the master male mold.
  • Shizuka Miyata; Choong Sik Park; Yusuke Sando; Yuta Fujiki; Kazuhiro Saeki; Nobuhiro Kato
    ADVANCED BIOMEDICAL ENGINEERING JAPANESE SOC MEDICAL & BIOLOGICAL ENGINEERING 5 (5) 83 - 87 2187-5219 2016 [Refereed]
     
    A simple time-lapse cinematography (TLC) device was developed to enable detection, at the cleavage stages, of embryos that have the potential to develop into high-scoring blastocysts. The TLC device, which consists of an optical microscope system, an illumination system, a PC-controlled camera, and a focus adjustment unit, is encapsulated in a waterproof container to allow operation in a gas-controlled water jacket incubator. Operation of the device in a high humidity environment was ensured by an environmental test in an incubator. By capturing the images of bovine embryos every 10 min for several days, their growing process was precisely recorded. The feature quantities calculated from the temporal difference images were utilized to predict the cleavage timings of the embryos.
  • Nobuhiro Kato; Yuki Ido; Ei Yamamoto; Kenzo Yasuo; Kazushi Yoshikawa; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 696 99 - 102 1013-9826 2016 [Refereed]
     
    © 2016 Trans Tech Publications, Switzerland. Dentin hypersensitivity is induced by mechanical stimuli or heat stimuli applied via exposed dentin tubules. Resin coating and laser-irradiation are used for treatment but have some problems such as poor biocompatibility or difficulty for laser power setting. We have been developing a treatment that creates artificial tooth enamel by attaching flexible ultrathin calcium phosphate sheet to seal the dentin tubule orifice. In this study, the quantitative evaluation of dentinal permeability inhibition rate (DPIR) improved by attaching ultrathin amorphous calcium phosphate (APC) sheets on human dentin in vitro is presented. The obtained DPIR by overlaid APC sheets application was 81.1 ± 5.7 %.
  • Ei Yamamoto; Nobuhiro Kato; Kazushi Yoshikawa; Kenzo Yasuo; Kazuyo Yamamoto; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 696 69 - 73 1013-9826 2016 [Refereed]
     
    © 2016 Trans Tech Publications, Switzerland. Tooth enamel cannot be naturally reconstructed once it is destroyed immoderately. Therefore, the restoration of enamel is important in dental treatment. Hydroxyapatite (HAp) can potentially be used for a novel dental biomaterial to repair the enamel. We have proposed a tooth restoration technique with intraoral laser ablation method. A thin layer of HAp was deposited on dentin surface in the atmosphere by ablation phenomenon using an Er:YAG (Erbium: Yttrium-Aluminum-Garnet) laser irradiated to the target of α-Tricalcium phosphate (α-TCP). Then, the deposited layer was hydrolyzed by dripping pure water on its surface in order to create HAp film. The adhesive strength between the HAp film and dentin was evaluated by quasi-static tensile tests. As a result of the mechanical evaluation, the adhesive strength was greater than approximately 3.8 MPa. It is clear that the HAp film created by the Er:YAG laser ablation method is attached tightly to the dentin.
  • 谷口俊仁; 加藤暢宏; 佐伯和弘
    畜産技術 畜産技術協会 (727) 22 - 25 0389-1348 2015/12
  • HONTSU Shigeki; YAMAMOTO Ei; KATO Nobuhiro; YOSHIKAWA Kazushi; YASUO Kenzo; YAMAMOTO Kazuyo
    Journal of Japanese Society for Laser Dentistry Japanese Society for Laser Dentistry 26 (1) 10 - 16 0917-7450 2015/04 
    Ideal restoration of tooth substances requires hydroxyapatite (HAp), of which the tooth is composed. However, restoration treatment with HAp is not yet possible, as adherence to ceramics is very difficult. In the present study, we examined a tooth restoration technique using the intraoral laser ablation method. A thin layer of α-tricalcium phosphate (α-TCP) was deposited on the dentin surface by the ablation phenomenon using an Er:YAG laser, which has already been introduced into dental treatment, to irradiate the target α-TCP. The deposited layer was then hydrolyzed by dripping pure water on its surface in order to create a hydroxyapatite (HAp) coating. In this study, we developed a compact pulsed laser deposition (PLD) system using the Er:YAG laser. The interface structure between the HAp coating and the dentin surface was observed by scanning electron microscope (SEM). Electron micrographs showed that the HAp layer was formed on the dentin surface. Moreover, dentinal tubules were sealed with HAp particles. We evaluated the blockade effect of dentinal tubules using Pashle's method. The measured average value for the sealing rate of the dentinal tubules was 85.6±8.6%, which was greater than the value with resin coating treatment. The adhesive strength of HAp films deposited on dentin was evaluated by quasi-static tensile tests. On mechanical evaluation, the adhesive strength was greater than 3.8 MPa. The present results suggest that this technique will be useful for the repair of dentin and the treatment of hyperaesthesia.
  • Nobuhiro Kato; Arata Isai; Ei Yamamoto; Hiroaki Nishikawa; Masanobu Kusunoki; Kazushi Yoshikawa; Kenzo Yasuo; Kazuyo Yamamoto; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 631 258 - 261 1013-9826 2015 [Refereed]
     
    © (2015) Trans Tech Publications, Switzerland. Dentin hypersensitivity is induced by mechanical stimuli or heat stimuli applied via dentin tubules exposed by lost of dental enamel or gingival recession. Common treatments for dentin hypersensitivity are resin coating or laser irradiation. However, these treatments have some problems such as poor biocompatibility or insufficient durability. We have been developing a treatment that creates artificial tooth enamel by attaching flexible ultrathin calcium phosphate sheet having a crystal structure similar to that of tooth enamel to seal the dentin tubules. In this study, the quantitative evaluation of dentin tubule sealing rate improved by attaching ultrathin amorphous calcium phosphate (ACP) sheet on human dentin is presented. The obtained sealing-rate by APC sheet application was 70.9 ±4.8%.
  • Ei Yamamoto; Nobuhiro Kato; Arata Isai; Hiroaki Nishikawa; Yoshiya Hashimoto; Kazushi Yoshikawa; Shigeki Hontsu
    Key Engineering Materials Trans Tech Publications Ltd 631 262 - 266 1013-9826 2015 [Refereed]
     
    © (2015) Trans Tech Publications, Switzerland. It has been well known that bone has piezoelectric properties and these properties have been considered to be caused by the shift of the center of symmetry of the positive and negative electrical charge due to the strain of the collagen fibers included in the bone. Thus, it has long been considered that there were no piezoelectric effects in the hexagonal hydroxyapatite(HAp) which has center of symmetry of crystal. However, in recent years, the piezoelectric property of artificially synthesized HAp was reported. In the authors' previous report, a new result which showed the piezoelectricity of the hydroxyapatite(HAp) films fabricated by the pulsed laser deposition(PLD) method was reported. In this study, the effect of poling treatment on piezoelectric constant of pulsed laser deposited HAp films was investigated.
  • Nobuhiro Kato; Takahisa Kai; Masakazu Hirano
    Journal of Photopolymer Science and Technology TECHNICAL ASSOC PHOTOPOLYMERS,JAPAN 27 (1) 85 - 89 0914-9244 2014/08 [Refereed]
     
    © 2014SPST. Backside lithography with a moving mask UV exposure technique is proposed. A novel moving-mask exposure apparatus was developed and evaluated both experimentally and through exposure simulations. The backside exposure using this technique was validated and is capable of producing tapered microstructures of thick photoresist for molding. The shape of the structure can be modified by the trajectory of the stage movement. It was confirmed that the shape of the processed structure could be successfully predicted using the proposed simulation method.
  • Hontsu Shigeki; Kato Nobuhiro; Yamamoto Ei; Yoshikawa Kazushi; Hashimoto Yoshiya; Nishikawa Hiroaki; Kusunoki Masanobu
    Journal of Bio-Integration Bio Integration Society 4 (1) 65 - 69 2186-2923 2014 [Refereed]
     
    We propose a new method for fabricating the hydroxyapatite (HAp) film in the atmosphere by the laserablation method using Er:YAG laser. The HAp films were deposited on the Ti substrate by ablation reactionby the Er:YAG laser irradiated to target of several kinds of calcium phosphate compounds. Subsequently, thedeposited film was changed to a material with HAp like structure by hydrolysis reaction with the pure waterin film surface. It became clear that α-tricalcium phosphate (α-TCP) had the early sedimentation rate andthat it changes to the HAp structure within 48 hours. Further, it was proven that the film became the HApstructure in about 3 hours, when it formed a TCP films by applying monocalcium phosphate solution with thepH 5.5 on the enamel surface. The interface of enamel and deposited layer is observed by scanning electronmicroscope (SEM), and it was confirmed that dense HAp film with the thickness of about 10～20μm had beenformed on the enamel. It seems that the technique becomes the technology which will be very useful for therepair of the dentine in future.
  • Nobuhiro Kato; Takahisa Kai; Masakazu Hirano
    JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY TECHNICAL ASSOC PHOTOPOLYMERS,JAPAN 27 (1) 85 - 89 0914-9244 2014 [Refereed]
     
    Backside lithography with a moving mask UV exposure technique is proposed. A novel moving-mask exposure apparatus was developed and evaluated both experimentally and through exposure simulations. The backside exposure using this technique was validated and is capable of producing tapered microstructures of thick photoresist for molding. The shape of the structure can be modified by the trajectory of the stage movement. It was confirmed that the shape of the processed structure could be successfully predicted using the proposed simulation method.
  • Restration of Tooth Enamel Using a Flexible Hydroxyapatite Sheet Coated with Tricalsium Phoshate
    Yamamoto; E. Kato; N Isai; A. Nishikawa; H. Kusunoki; M. Yoshikawa; K. Hontsu, S
    Bioceramics Development and Applications S1 006  2013/08 [Refereed]
  • Ultrathin Amorphous Calcium Phosphate Freestanding Sheet for Dentin Tublue Sealing
    Kato; N Yamamoto; E. Isai; A. Nishikawa; H. Kusunoki; M. Yoshikawa; K. Hontsu, S
    Bioceramics Development and Applications S1 007  2013/08 [Refereed]
  • E. Yamamoto; N. Kato; H. Nishikawa; M. Kusunoki; T. Hayami; K. Yoshikawa; S. Hontsu
    Key Engineering Materials TRANS TECH PUBLICATIONS LTD 529-530 (1) 522 - 525 1013-9826 2013 [Refereed]
     
    Tooth enamel cannot be reconstructed once it is destroyed immoderately. Hydroxyapatite (HAp) thin sheet can potentially be used for a novel dental biomaterial to repair the enamel. Using a pulsed laser deposition (PLD) method, we have successfully created a flexible HAp sheet of less than a few micrometers in thickness. Due to its flexibility, the HAp sheet is tightly adhered on curved surfaces at the target site. In the present study, we newly developed double-layered sheets composed of HAp film coated with tricalcium phosphate (TCP) thin layer. The HAp/TCP sheet was adhered to the extracted human teeth using a calcium phosphate solution for 3 days. The adhesive strength between the HAp/TCP sheet and tooth enamel was evaluated by quasi-static tensile tests. Moreover, the interface structure between them was observed by a scanning electron microscopy. As a result of the mechanical evaluation, the adhesive strength was greater than approximately 2.5 MPa. The electron microscopic observation revealed that the sheet was partially fused with the enamel. These findings suggest the possibility that enamel defects are repaired using the HAp/TCP sheet for a short duration. © (2013) Trans Tech Publications, Switzerland.
  • Shu Hashimoto; Nobuhiro Kato; Kazuhiro Saeki; Yoshiharu Morimoto
    Fertility and Sterility ELSEVIER SCIENCE INC 97 (2) 332 - 337 0015-0282 2012/02 [Refereed]
     
    Objective: To assess the developmental kinetics of human embryos and their ability to develop to morphologically normal blastocysts. Design: Experimental study on human embryos donated for research using a time-lapse imaging system based on individual embryo culture in poly(dimethylsiloxane) microwells and monitored using a microscope inside the incubator. Setting: Private fertility clinic. Patient(s): Surplus embryos donated by couples after undergoing fertility treatment. Intervention(s): None. Main Outcome Measure(s): Blastocyst score and times required from beginning to completion of the second and third mitotic divisions. Result(s): The time required for completion of the second division (the three- to four-cell stage) was shorter in embryos that developed to high-scoring blastocysts (0.7 hours, n = 17) than in those forming low-scoring blastocysts (3.7 hours, n = 24). Similarly, the mean time required to completion of the third division (five- to eight-cell stage) was also significantly shorter in embryos forming high-scoring blastocysts (5.7 hours) than among those forming low-scoring blastocysts (16.9 hours). Conclusion(s): Individual embryos with the potential to develop to high-scoring blastocysts could be selected at 2-3 days of culture using this system by examining the times required to complete the second and third mitotic divisions. © 2012 American Society for Reproductive Medicine, Published by Elsevier Inc.
  • S. Hontsu; N. Kato; E. Yamamoto; H. Nishikawa; M. Kusunoki; T. Hayami; K. Yoshikawa
    Key Engineering Materials Key Engineering Materials 493-494 615 - 619 1013-9826 2012 [Refereed]
     
    Before a tooth erupts, the ameloblasts are lost, which means that the tooth enamel does not regenerate itself after tooth eruption. In the present study, we attempt to regenerate the tooth enamel artificially using a flexible hydroxyapatite (HAp) sheet. First, a HAp film was deposited on a soluble substrate by pulsed laser deposition (PLD) using an ArF excimer laser. Next, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. The HAp sheet was adhered to the extracted human teeth using a calcium phosphate solution. The variation of the crystal structure of the HAp sheet with time was investigated by X-ray diffraction analysis. Furthermore, the variation in the mechanical characteristics with time between the HAp sheet and dental enamel were evaluated using tensile and scratch tests. The results suggest that the HAp sheet became fused to the tooth enamel within approximately one week. © (2012) Trans Tech Publications.
  • 橋本 周; 佐伯 和弘; 加藤 暢宏; 森本 義晴
    産婦人科の実際 金原出版(株) 60 (7) 1039 - 1044 0558-4728 2011/07
  • Kazuhiro Saeki; Nobuhiro Kato; Daisaku Iwamoto; Junki Sho; Masao Kishi; Kazuya Matsumoto; Yoshihiko Hosoi; Akira Iritani
    BIOLOGY OF REPRODUCTION SOC STUDY REPRODUCTION 85 0006-3363 2011/07 [Refereed]
  • S. Hontsu; K. Yoshikawa; N. Kato; Y. Kawakami; T. Hayami; H. Nishikawa; M. Kusunoki; K. Yamamoto
    Journal of the Australian Ceramic Society AUSTRALIAN CERAMIC SOCIETY 47 (1) 11 - 13 0004-881X 2011 [Refereed]
     
    A freestanding hydroxyapatite (HAp) sheet of 5 ∼ 10 μm thickness was successfully prepared by a novel method. After depositing the thin HAp film on a soluble substrate by pulsed laser deposition (PLD) technique, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. Thereafter, HAp sheets were directly attached to enamel and dentin surfaces of extracted human teeth. Artificial saliva was sprayed every third day, and the course was observed for 90 days. Observation of the enamel-HAp and dentin-HAp interface by scanning electron microscopy revealed that the HAp sheet had stuck to the enamel and dentin surface.
  • S. Hontsu; K. Yoshikawa; N. Kato; Y. Kawakami; T. Hayami; H. Nishikawa; M. Kusunoki; K. Yamamoto
    JOURNAL OF THE AUSTRALIAN CERAMIC SOCIETY AUSTRALIAN CERAMIC SOCIETY 47 (1) 11 - 13 0004-881X 2011 [Refereed]
     
    A freestanding hydroxyapatite (HAp) sheet of 5 similar to 10 mu m thickness was successfully prepared by a novel method. After depositing the thin HAp film on a soluble substrate by pulsed laser deposition (PLD) technique, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. Thereafter, HAp sheets were directly attached to enamel and dentin surfaces of extracted human teeth. Artificial saliva was sprayed every third day, and the course was observed for 90 days. Observation of the enamel-HAp and dentin-HAp interface by scanning electron microscopy revealed that the HAp sheet had stuck to the enamel and dentin surface.
  • 森本 康一; 西山 裕之; 國井 沙織; 角田 昌明; 加藤 暢宏
    Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University 近畿大学生物理工学部 (26) 39 - 46 1342-7202 2010/09 
    本研究は近畿大学生物理工学部戦略的研究No.06-II-1, 2006およびNo.07-IV-3, 2007の助成を受けた,灌流培養環境下でHL60細胞とI型コラーゲンとの相互作用を観察するため, poly (dimethylsiloxane)を用いて微小流路デバイス(幅700μm×高さ100μm×長さ20mm)を作製した. 矩形断面を有する微小流路内壁4面すべてを酸素プラズマ処理し, ニワトリI型コラーゲンとウシ胎児血清成分タンパク質を灌流して固定化した. 固定化されたタンパク質の吸着量をSDS-ポリアクリルアミドゲル電気泳動にて定量的に調べた結果, 血清タンパク質に対して酸素プラズマ処理の効果は認められなかったが, I型コラーゲンでは酸素プラズマ処理の効果が経時的に減少するに従い, その固定化量が増加した. 作製した微小流路デバイスを用いた0.2μL/minの灌流培養下で, HL60細胞はI型コラーゲンにのみローリングあるいは接着挙動を示した. 酸素プラズマ処理の効果はI型コラーゲンに対する細胞接着に大きく影響した. HL60細胞の接着挙動は固定化されたI型コラーゲン量に単純に比例せず, その構造変化を認識することが示唆された. (英文) We developed microfluidic device (W 700μm×H 100 gμm×L 20 mm) with poly (dimethylsiloxane) to observe adhesion of HL60 cells on type I collagen under continuous perfusion. The surface of poly (dimethylsiloxane) was treated by oxygen plasma and then coated by fetal calf serum proteins or chicken type I collagen. By using SDS-polyacrylamide gel electrophoresis, we showed that treatment of oxygen plasma had no effect on binding of serum proteins on the polymer surface. However, the amount of collagen on the surface increased by decreasing of the effect of plasma treatment. On the serum protein-coated polymer surface, HL60 cells fully flowed through under 0.2μL/min flow rate. On the other hand, HL60 cells bound to the collagen-coated polymer surface and subsequently adhered to the collagen-coated polymer surface. We suggest that the conformation of collagen would alter depending on the degree of oxygen plasma treatment of PDMS and HL60 cells would recognize the difference in collagen structure.
  • S. Hashimoto; N. Kato; K. Saeki; Y. Morimoto
    HUMAN REPRODUCTION OXFORD UNIV PRESS 25 I191 - I191 0268-1161 2010/06
  • Yoko Kamotani; Tommaso Bersano-Begey; Nobuhiro Kato; Yi Chung Tung; Dongeun Huh; Jonathan W. Song; Shuichi Takayama
    Biomaterials ELSEVIER SCI LTD 29 (17) 2646 - 2655 0142-9612 2008/06 [Refereed]
     
    Cell culture systems are often static and are therefore nonphysiological. In vivo, many cells are exposed to dynamic surroundings that stimulate cellular responses in a process known as mechanotransduction. To recreate this environment, stretchable cell culture substrate systems have been developed, however, these systems are limited by being macroscopic and low throughput. We have developed a device consisting of 24 miniature cell stretching chambers with flexible bottom membranes that are deformed using the computer-controlled, piezoelectrically actuated pins of a Braille display. We have also developed efficient image capture and analysis protocols to quantify morphological responses of the cells to applied strain. Human dermal microvascular endothelial cells (HDMECs) were found to show increasing degrees of alignment and elongation perpendicular to the radial strain in response to cyclic stretch at increasing frequencies of 0.2, 1, and 5 Hz, after 2, 4, and 12 h. Mouse myogenic C2C12 cells were also found to align in response to the stretch, while A549 human lung adenocarcinoma epithelial cells did not respond to stretch. © 2008 Elsevier Ltd. All rights reserved.
  • FUJII Masao; KATO Nobuhiro; SHIMIZU Takaki; SATOMI Kenichi; TAGUCHI Shinya
    Transactions of the Japan Society of Mechanical Engineers C The Japan Society of Mechanical Engineers 73 (725) 30 - 35 0387-5024 2007/01 
    3D-CAD system has been used in practical product design to realize simultaneous engineering and also to improve productivity. Although 3D-CAD is a widely used and highly effective tool in mechanical design, it also has its drawbacks: mastery of 3D-CAD skills is rather complex and time-consuming. In an attempt to simplify the learning process and cut down on learning time, we have developed training programs for mechanical design such as a concept making, a family tree and a team design method, and e-Learning contents for the 3D-CAD operation skills. The effectiveness of these training program and e-Learning contents has been confirmed by cooperative activity between an enterprise and a school in mechanical design education.
  • Masao Fujii; Nobuhiro Kato; Takaki Shimizu; Kenichi Satomi; Shinya Taguchi
    Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C 73 (1) 30 - 35 0387-5024 2007/01 [Refereed]
     
    3 D-CAD system has been used in practical product design to realize simultaneous engineering and also to improve productivity. Although 3 D-CAD is a widely used and highly effective tool in mechanical design, it also has its drawbacks : mastery of 3 D-CAD skills is rather complex and time-consuming. In an attempt to simplify the learning process and cut down on learning time, we have developed training programs for mechanical design such as a concept making, a family tree and a team design method, and e-Learning contents for the 3 D-CAD operation skills. The effectiveness of these training program and e-Learning contents has been confirmed by cooperative activity between an enterprise and a school in mechanical design education.
  • 加藤 暢宏; 藤井 雅雄; 田口 晋也
    論文誌IT活用教育方法研究 私立大学情報教育協会 8 (1) 46 - 50 1880-6406 2005/11
  • 佐伯 和弘; 住友 範生; 永田 由紀; 加藤 暢宏; 細井 美彦; 松本 和也; 入谷 明
    The Journal of Reproduction and Development (公社)日本繁殖生物学会 51 (2) 293 - 298 0916-8818 2005/04 
    The atomic force microscope (AFM) provides nanometer resolution, topographic data of the natural surface structure of materials. We studied the topology of the surface structure of bovine sperm heads during the acrosome reaction by AFM. In addition, we numerically analyzed the areas of the median sagittal plane of the sperm heads. Bovine frozen-thawed spermatozoa were washed, capacitated by heparin, and incubated with lysophosphatidylcholine (LPC) to induce the acrosome reaction, smeared on a cover glass, air-dried, and observed with AFM using the dynamic force (tapping) mode. AFM analysis of spermatozoa showed the clear surface structure of acrosomes, equatorial segments, postacrosomal regions and necks. Although AFM images of spermatozoa capacitated by heparin had complete acrosomes, most spermatozoa treated with LPC had no acrosomal caps as shown by AFM. These observations coincided with those obtained by light microscopy after staining with naphthol yellow S and erythrosin B. Furthermore, numerical analysis of AFM images indicated that areas of the median sagittal plane of the anterior portions of acrosome-reacted sperm heads (2679 ± 616 pixels) were approximately 40% less than those of intact heads (4535 ± 174 pixels, P<0.05). These results indicate that AFM can usefully observe and numerically analyze the fine surface structures of bovine spermatozoa.
  • Nobuhiro Kato; Isao Kohno; Hisao Kikuta
    Korea Research Institute of Standards and Science (KRISS) - Asia-Pacific Symposium on Measurement of Mass, Force and Torque, APMF 2005 95 - 98 2005 [Refereed]
     
    We report on the improvement in the fabrication process of the polymer micro cantilevers for force controlled atomic force microscope. In the previous paper we reported on the preliminary results of fabrication of the micro cantilevers using SU-8, the photo resist for MEMS. The problem in conventional fabrication process are the probe tip mold failure, mask sticking and time consuming dissolving process of sacrifice layer. To overcome these difficulties we improved mask pattern for probe tip, cantilever and base. The mask pattern for probe tip is changed form square to circle. Using several sizes of circles we estimate the effect of under cut of anisotropic etching by TMAH. The mask sticking is eliminated by refined heat treatment after the soft baking process. To accelerate the sacrifice layer etching, the base and cantilever mask pattern is perforated. The sacrifice layer material is also changed. We find that the 0.6 μm thick gold on 30nm thick chromium is the appropriate material for sacrifice layer. Using this alternative process, we developed the fully plastic micro cantilevers with good probe reproducibility.
  • 萩野 秀樹; 朴 忠植; 加藤 暢宏
    レーザ加工学会誌 レーザ加工学会 11 (3) 149 - 153 2004/12
  • 萩野 秀樹; 加藤 暢宏; PARK C S; 三俣 真理; 田; 久雄; 岩田 耕一
    大阪府立産業技術総合研究所報告 大阪府立産業技術総合研究所 (18) 87 - 91 2004/11 [Refereed]
     
    レーザ加工を行う場合に,レーザ光の強度分布を加工対象に適した分布にすることによって,加工品質や加工効率を向上させることができる。本研究では,高出力炭酸ガスレーザ光の強度分布を所望の分布に整形する回折型光学素子の開発を行った。素子は高出力レーザに使われる銅の放物面鏡上に,2レベルの計算機ホログラム(CGH)を作製したものである。CGHは,段差3.75μm,一辺が80μmの正方形のピクセル256×256個で構成されている。本素子は,高出力レーザに適用する際の冷却特性を上げるために素材として銅を選択した。また,基板を放物面鏡にすることで,一つの素子に集光の機能とビーム整形の機能を持たせることを容易にした。素子の作製では,CGHパターンの描画に3次元レーザ描画装置を用い,凹凸形状の作製にエッチストップ層を用いたエッチングプロセスを利用した。実際に,ガウス分布の炭酸ガスレーザ光を,一方向には一様強度,その直交方向にはガウス分布をした矩形形状に整形する素子
  • HAGINO Hideki; PARK Choong Sik; YOKOI Masayuki; KATO Nobuhiro; MITSUMATA Mari; KIKUTA Hisao; IWATA Koichi
    Journal of the Japan Society for Precision Engineering, Contributed Papers The Japan Society for Precision Engineering 70 (9) 1180 - 1185 1348-8724 2004/09 
    A multilevel computer generated hologram (CGH) was fabricated for shaping a laser beam profile of a high-power CO2 laser. The surface relief has eight height levels with 0.94μm in step heights. A copper surface relief was formed on a flat copper substrate by electroplating with photo-resist masks. Standard deviation of the surface step height was less than 22% of the designed height. The CGH changes a CO2 laser beam of 14mm in diameter to a rectangular beam of 1mm×4mm and its diffraction efficiency was 69.2%. A 200W CO2 laser beam of 14mm in diameter did not damage the CGH surface.
  • FUJII M.; KATO Nobuhiro; SHIMIZU Takaki; SATOMI Kenichi
    Memories of The School of B. O. S. T. of Kinki University Kinki University 14 (14) 101 - 108 1342-7202 2004/09 
    The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. Particularly, a three dimensional CAD(3D-CAD) system has been used in practical product design to realize simultaneously engineering and also to improve the productivity. At an enterprise, mechanical engineers have been required to master 3D-CAD as soon as possible. On the other hand, many students graduating from a school will be engaged in the enterprise so that they should study 3D-CAD. Therefore we have been cooperating for 3D-CAD education and confirmed its effectiveness.
  • Matsumoto Toshiro; Kojima Arata; Izuchi Noboru; Kato Nobuhiro; Watanabe Toshiaki
    Memoirs of the School of Biology-Oriented Science and Technology of Kinki University Kinki University 13 (13) 73 - 78 1342-7202 2004/02 
    The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. Double exposures and real-time holographic interferometry were used to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to simulate properly the situation of a femur under physiological muscles, the 3D finite element method (FEM) was used for our analyses. After modeling the femur, which consisted of the meshes as eight noded elements for each section of diaphysis, stress and strain were calculated by means of the 3D FEM. The following results were obtained : (1) the deformations increased with the progress of osteoporosis, (2) femur with osteoporosis showed obvious rotational components around the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • N. Kato; C. S. Park; T. Matsumoto; H. Kikuta; K. Iwata
    Proceedings of the SICE Annual Conference SOC INSTRUMENT CONTROL ENGINEERS JAPAN 1851 - 1854 2004 [Refereed]
     
    In this paper, we report on the improvements in fabrication processes of polymer micro cantilevers for force controlled atomic force microscopy. The improvement of photo mask patterns results in the enhancement of controllability of the pyramidal probe tip geometry and in rapid dissolving of sacrificial layer. It also contributes to process simplicity.
  • FUJII Masao; KATO Nobuhiro
    Journal of Japan Society for Design Engineering 日本設計工学会 38 (8) 377 - 382 0919-2948 2003/08
  • Hideki Hagino; Choong Sik Park; Nobuhiro Kato; Mari Mitsumata; Hisao Kikuta; Koichi Iwata
    Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering The Japan Society for Precision Engineering 69 (3) 417 - 421 0912-0289 2003/03 [Refereed]
     
    This paper describes a computer generated hologram (CGH) on a curved surface for laser machining. The CGH fabricated on a parabolic copper mirror converts a 14mm diameter Gaussian beam of a CO2 laser into a shaped beam whose intensity distribution is uniform (4mm) in x direction and gaussian (1mm) in y direction. The binary CGH pattern was drawn by a laser direct writing machine with a focus servo mechanism. To obtain an accurate step height of CGH pattern, a palladium thin film was placed between the copper mirror and a plated copper layer 3.75μm thick. While the plated layer was etched by a ferric chloride solution, the palladium film prevented the substrate mirror from being etched. The beam shaping by the fabricated CGH was demonstrated experimentally with a low power CO2 laser and an infrared-camera.
  • Nobuhiro Kato; Choong Sik Park; Toshiro Matsumoto; Hisao Kikuta; Koichi Iwata
    Proceedings of SPIE - The International Society for Optical Engineering SPIE-INT SOC OPTICAL ENGINEERING 5183 71 - 78 0277-786X 2003 [Refereed]
     
    We developed the fabrication process of all-plastic cantilever for force controlled atomic force microscope. The force controlled atomic force microscope can prevent damage during the scanning of the soft sample. However it has a drawback in its stability. To stabilize the system the cantilever is required to have small Q factor. The negative photo resist for MEMS (SU-8) has ideal properties, micromachinability and small Q factor, for this purpose. Here we demonstrate the cantilever consists of tip, lever and base with same polyimide. The mold of the probing tip is the pyramidal pit pattern on silicon wafer formed by direct leaser writing and anisotropic etching of silicon. Other elements of the cantilever are formed by contact lithography. The photo mask is also fabricated by direct laser writing. By arranging the dimensions of the cantilever, we can control its spring constant and resonant frequency. For the spring constant of 0.1N/m and the first resonant frequency of 2kHz, the typical dimensions of the cantilever is 1030 × 300 × 7 μm3. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • Hagino H; Park C.S; Kato N; Mitsumata M; Kikuta H; Iwata K
    Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering The Japan Society for Precision Engineering 69 (3) 417 - 421 0912-0289 2003 [Refereed]
     
    This paper describes a computer generated hologram (CGH) on a curved surface for laser machining. The CGH fabricated on a parabolic copper mirror converts a 14mm diameter Gaussian beam of a CO 2 laser into a shaped beam whose intensity distribution is uniform (4mm) in x direction and gaussian (1mm) in y direction. The binary CGH pattern was drawn by a laser direct writing machine with a focus servo mechanism. To obtain an accurate step height of CGH pattern, a palladium thin film was placed between the copper mirror and a plated copper layer 3.75μm thick. While the plated layer was etched by a ferric chloride solution, the palladium film prevented the substrate mirror from being etched. The beam shaping by the fabricated CGH was demonstrated experimentally with a low power CO 2 laser and an infrared-camera.
  • FUJII M.; Kato Nobuhiro
    Memories of The School of B. O. S. T. of Kinki University Kinki University 11 (11) 136 - 155 1342-7202 2002/12 
    The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. This paper describes the progress of mechanical design technology and the importance of the education by using 3dimensional computer aided design (3DCAD) system at multimedia age. In the product design, it is important to promote the creative and intelligent design operation and also to construct the effective educational curriculum by using 3DCAD.
  • H Kikuta; K Numata; H Arimitsu; K Iwata; N Kato
    SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5 IEEE 2510 - 2511 2002 [Refereed]
     
    We developed a 92x70 micro retarder array for an imaging polarimetry. The micro retarder array consists of polyimide subwavelength-period gratings of 300nm period with different orientations. The phase retardance is caused by the form birefringence of the subwavelength structure. The gratings were fabricated with the direct-writing electron-beam lithography and the reactive ion etching. The phase retardance was 0.5pi for the 550nm light wavelength. We have observed a birefringence image of a tensed plastic film by using the imaging polarimetry system.
  • N Kato; CS Park; T Matsumoto; H Kikuta; K Iwata
    SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5 IEEE 1033 - 1034 2002 [Refereed]
     
    The micro cantilever made of entirely plastic material has been developed. The probing tip, cantilever and base are made of same photo plastic. By arranging the geometry of the cantilever, its spring constant and resonant frequency is controlled. The low Q of the polyimide improves the stability of the force controlled atomic force microscope. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • KATO Nobuhiro; MATSUMOTO Toshiro; KIKUTA Hisao; NAKAMURA Yasuhiro; IWATA Koichi
    Japanese Journal of Applied Physics The Japan Society of Applied Physics 40 (11) 6594 - 6599 0021-4922 2001/11 
    In this paper we present a method of reducing the bandwidth required in the force-controlled atomic force microscope (AFM) for measuring the force curves. System stability strongly depends on the quality factor of vibration (Q) of the cantilever. As polyimide has a low Q, a cantilever fabricated from it reduces the bandwidth required in the feedback system. A polyimide cantilever with dimensions of 540 × 155 × 3.4 μm3 is fabricated to evaluate its Q in vacuum, density and Young's modulus. The measured Q is 50, which is much smaller than those of conventional cantilevers used for AFM. Using the measured mechanical properties of the polyimide, the required frequency response of the feedback system is estimated. Typical force curve measurements are confirmed by some numerical simulations.
  • Nobuhiro Kato; Toshiro Matsumoto; Hisao Kikuta; Yasuhiro Nakamura; Koichi Iwata
    Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers INST PURE APPLIED PHYSICS 40 (11) 6589 - 6593 0021-4922 2001/11 [Refereed]
     
    Tungsten oxide (WO3) thin films have shown interesting properties as lithography resist materials. In this study, the scanning tunneling microscope (STM) was used in air for nanometer etching of α-WO3-x thin films, an n-type semiconductor. The current-voltage (I-V) curve was found to be affected by the water layer absorbed on the surface. For low voltage (< 3 V) with long duration (∼ 10s) or high voltage pulse (> 3 V) with short pulsewidth (∼ 200 ms), holes were formed on the film surface at high humidity (> 70%) by applying a pulse voltage. A threshold voltage existed for hole formation. Higher pulse voltage and negative polarity corresponded to larger modified size. All the structures formed in STM images were topographical in nature by comparison with the AFM images. The hole formation was reasonably attributed to electrochemistry and high dissolution of WO3 in high pH solutions, which was co-manifested by links between the holes and eroded materials on the surface. Alkaline solutions instead of deionized water were chosen to act in the tip-surface gap. Alkaline ions being expelled from the tip due to electric polarity demonstrated the ion movement and their effect on etching. Lines of nanometer width were fabricated.
  • N Kato; T Matsumoto; H Kikuta; Y Nakamura; K Iwata
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS JAPAN SOC APPLIED PHYSICS 40 (11) 6594 - 6599 0021-4922 2001/11 
    In this paper we present a method of reducing the bandwidth required in the force-controlled atomic force microscope (AFM) for measuring the force curves. System stability strongly depends on the quality factor of vibration (Q) of the cantilever. As polyimide has a low Q. a cantilever fabricated from it reduces the bandwidth required in the feedback system. A polyimide cantilever with dimensions of 540 x 155 x 3.4 mum(3) is fabricated to evaluate its Q in vacuum. density and Young's modulus. The measured Q is 50, which is much smaller than those of conventional cantilevers used for AFM. Using the measured mechanical properties of the polyimide, the required frequency response of the feedback system is estimated. Typical force curve measurements are confirmed by some numerical simulations.
  • T. Matsumoto; A. Kojima; N. Kato; T. Watanabe; M. Tamiwa; M. Baba
    Annual International Conference of the IEEE Engineering in Medicine and Biology-Proceedings 2 1508 - 1510 0589-1019 2001 [Refereed]
     
    The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1) the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • T Matsumoto; A Kojima; T Watanabe; M Tamiwa; M Baba
    PROCEEDINGS OF THE 23RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-4 IEEE 23 1508 - 1510 1094-687X 2001 [Refereed]
     
    The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1)the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • Nobuhiro Kato; Hisao Kikuta; Takashi Nakano; Toshiro Matsumoto; Koichi Iwata
    Review of Scientific Instruments 70 2402 - 2407 0034-6748 1999/12 [Refereed]
     
    The force-feedback method is a promising technique to measure accurate force curves in atomic force spectroscopy. In this article, we describe criteria of the stability for the force-feedback system containing a proportional amplifier with first-order lag. We derive the criteria from an equation of the cantilever movement with higher-order vibration modes under force interaction. The criteria predict that a proportional amplifier with a certain gain and a "very high" cutoff frequency is required to stabilize the feedback system when the critical force gradient exceeds the cantilever stiffness. To measure a force curve, including the steep part (-17 N/m) with a soft (0.2 N/m) and poor-damping (2 X 10-7 kg/s) cantilever, the amplifier for the force-feedback controller must have a frequency response wider than 16 MHz and gain larger than 16.8. Moreover, the feedback controller with only an integrator, which is popular in scanning tunneling microscopy and atomic force microscopy, does not contribute to reducing the discontinuity of the force curve. © 1999 American Institute of Physics.
  • 佐伯 和弘; 加藤 暢宏; 細井 美彦; 松本 和也; 加藤 博己; 入谷 明
    Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University 近畿大学生物理工学研究所 (2) 26 - 32 1344-414X 1999/03 
    継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University走査型近視野原子間力顕微鏡は、観察したい材料に処理を加えずに、そのままの表面形状を nanometer単位で非常に微細に観察でき、容易にその3次元構築像が得られる。今回この顕微鏡を用いて凍結保存されたウシ精子を融解後、heparinによる受精能獲得処理さらにlysophosphatidylcholine (LC) による先体反応誘起処理を行い、その頭部の表面形状を観察した。融解直後、Percoll洗浄後およびheparin処理後の精子は先体帽、先体赤道部、後核帽および頚部が観察された。洗浄処理や heparin処理により精子表面の形状には変化が見られなかった。LC処理後の精子は、先体が観察できず頭部先端部分に胞状化した先体の一部あるいは先体顆粒の結晶と思われる粒子が数多く付着している像が観察され、LC処理により先体反応が誘起されたと思われた。また、いずれのサンプルでも、多くの結晶構造が精子付近あるいは精子表面上に観察された。これは、精子を培養液とともに直接スライドグラス上に塗沫・風乾したため塩類の結晶が形成されたためと推察された。 （英文） Atomic force microscope (AFM) provides nanometer-resolved, topographic data images of the natural surface structure of the objects. AFM also has a novel 3 D image-contrast mechanisms. In this study, surfaces of bovine sperm heads frozen-thawed, Percoll-washed, capacitated and acrosome-reacted were observed with the AFM. Spermatozoa were smeared on glass coverslip and air-dried. AFM images of spermatozoa after thawing and washing clearly showed their acrosomal caps, equatorial segments, postoacrosomal regions and necks. Images of capacitated spermatozoa treated with heparin also showed their complete acrosomal caps. However, acrosome-reacted spermatozoa induced by lysophosphatidylcholine were lack of their acrosome caps. Images also indicated salt crystals which were formed during airdrying smears of sperm suspension.
  • Nobuhiro Kato; Hisao Kikuta; Takashi Nakano; Toshiro Matsumoto; Koichi Iwata
    Review of Scientific Instruments AMER INST PHYSICS 70 (5) 2402 - 2407 0034-6748 1999 [Refereed]
     
    The force-feedback method is a promising technique to measure accurate force curves in atomic force spectroscopy. In this article, we describe criteria of the stability for the force-feedback system containing a proportional amplifier with first-order lag. We derive the criteria from an equation of the cantilever movement with higher-order vibration modes under force interaction. The criteria predict that a proportional amplifier with a certain gain and a "very high" cutoff frequency is required to stabilize the feedback system when the critical force gradient exceeds the cantilever stiffness. To measure a force curve, including the steep part (-17 N/m) with a soft (0.2 N/m) and poor-damping (2 X 10-7 kg/s) cantilever, the amplifier for the force-feedback controller must have a frequency response wider than 16 MHz and gain larger than 16.8. Moreover, the feedback controller with only an integrator, which is popular in scanning tunneling microscopy and atomic force microscopy, does not contribute to reducing the discontinuity of the force curve. © 1999 American Institute of Physics.
  • Formation of Biocompatible Hydroxyapatite Thin Films deposited by Laser Ablation (共著)
    Bio ceramics (Proc. 12th Int. Sympo. on Ceramics in Medicine) 12 499 - 502 1999
  • HIRAI Yoshihiko; MORI Ryosuke; KIKUTA Hisao; KATO Nobuhiro; INOUE Koji; TANAKA Yoshio
    Japanese Journal of Applied Physics The Japan Society of Applied Physics 37 (12) 7064 - 7069 0021-4922 1998/12 
    The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also, the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results.
  • Y Hirai; R Mori; H Kikuta; N Kato; K Inoue; Y Tanaka
    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS JAPAN J APPLIED PHYSICS 37 (12B) 7064 - 7069 0021-4922 1998/12 
    The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also. the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results.
  • Shigeki Hontsu; Masaya Nakamori; Nobuhiro Kato; Hitoshi Tabata; Junya Ishii; Toshirou Matsumoto; Tomoji Kawai
    Japanese Journal of Applied Physics, Part 2: Letters JAPAN J APPLIED PHYSICS 37 (10 PART A) L1169 - L1171 0021-4922 1998/10 [Refereed]
     
    An ArF excimer laser deposition technique is used to fabricate hydroxyapatitc [Ca10(PO4)6(OH)2]-(HAp) thin films on surface-modified polytetrafluoroethylene (PTFE) substrates. The surface of PTFE is modified by a sodium-naphthalene complex in a glycol ether solvent. X-ray diffraction (XRD) studies indicate that as-deposited films prepared at substrate temperatures below the glass transition temperature of PTFE (327°C) are in an amorphous state. The crystallization of as-deposited films needed annealing for 10h at 310°C. The structure, surface morphology and bond strength of HAp films were evaluated using XRD, the atomic force microscopy (AFM) and the Instron testing machine. The tensile bond strength of HAp films on surface-modified PTFE was 6.0 MPa, which is one order of magnitude larger than that of films on non-surface-modified PTFE, demonstrating its potential for practical applications.
  • HONTSU Shigeki; NAKAMORI Masaya; KATO Nobuhiro; TABATA Hitoshi; ISHII Junya; MATSUMOTO Toshirou; KAWAI Tomoji
    Japanese Journal of Applied Physics Pt. 2 Letters The Japan Society of Applied Physics 37 (10) L1169 - L1171 0021-4922 1998/10 
    An ArF excimer laser deposition technique is used to fabricate hydroxyapatite [Ca-10(PO4)(6)(OH)(2)]-(HAp) thin films on surface-modified polytetrafluoroethylene (PTFE) substrates. The surface of PTFE is modified by a sodium-naphthalene complex in a glycol ether solvent. X-ray diffraction (XRD) studies indicate that as-deposited films prepared at substrate temperatures below the glass transition temperature of PTFE (327 degrees C) are in an amorphous state. The crystallization of as-deposited films needed annealing for 10 h at 310 degrees C. The structure, surface morphology and bond strength of HAp films were evaluated using XRD, the atomic force microscopy (AFM) and the Instron testing machine. The tensile bond strength of HAp films on surface-modified PTFE was 6.0 MPa, which is one order of magnitude larger than that of films on non-surface-modified PTFE, demonstrating its potential for practical applications.
  • MATSUMOTO Toshiro; KATO Nobuhiro; TAGA Masao
    Japanese journal of applied physics Pt. 1 Regular papers, brief communications & review papers The Japan Society of Applied Physics 37 (9) 5088 - 5093 0021-4922 1998/09 
    The optimal physical properties of an ionized polyacrylamide solution, selected as the most suitable thickener for the insulating layer of a viscosity-stabilized solar pond, are described. Light transmittance, viscosity and viscosity degradation were obtained for a range of thicknesses from 1 cm to 15 cm (1-15 cm): weight concentrations of 0.1-0.5% (0.1-0.5 wt%) and temperatures of the polymer of 20-80 degrees C. Onset of convection was also measured for a given thickness of the polymer solution. An exposure test was carried out to prevent degradation of the polymer using an antioxidant. It was confirmed that the viscosity was not decreased and, on the contrary, increased slightly after 100 days. It was shown experimentally that the critical Rayleigh number coincided with the theoretical values reported by Tien rr of. [Int. J. Heat Mass Transf. 12 (1969) 1173]. Transmittances of the polymeric gel for the case of vessels greater than 5 cm in thicknesses were better than those of the SPR402 solution (0.5 wt%).
  • Toshiro Matsumoto; Nobuhiro Kato; Masao Taga
    Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers JAPAN J APPLIED PHYSICS 37 (9 A) 5088 - 5093 0021-4922 1998/09 [Refereed]
     
    The optimal physical properties of an ionized polyacrylamide solution, selected as the most suitable thickener for the insulating layer of a viscosity-stabilized solar pond, are described. Light transmittance, viscosity and viscosity degradation were obtained for a range of thicknesses from 1 cm to 15 cm (1-15 cm), weight concentrations of 0.1-0.5% (0.1-0.5 wt%) and temperatures of the polymer of 20-80°C. Onset of convection was also measured for a given thickness of the polymer solution. An exposure test was carried out to prevent degradation of the polymer using an antioxidant. It was confirmed that the viscosity was not decreased and, on the contrary, increased slightly after 100 days. It was shown experimentally that the critical Rayleigh number coincided with the theoretical values reported by Tien et al. [Int. J. Heat Mass Transf. 12 (1969) 1173]. Transmittances of the polymeric gel for the case of vessels greater than 5 cm in thicknesses were better than those of the SPR402 solution (0.5 wt%).
  • Matsumoto Toshiro; Nagae Sadahiko; Kato Nobuhiro
    Memoirs of the School of Biology-Oriented Science and Technology of Kinki University Kinki University 3 (3) 38 - 44 1342-7202 1998/06 
    A semi-quantitative measurement of the strain distribution by two-dimensional color dispaly is presented. The strain components are converted into color band intensity distributions in the image of the specimen. The method is based on a optical technique with the aid of the spatial filtering theory. To show a usefulness of the method, it was carried out to measure the strain distribution of a plane plate with a round hole.
  • Y. Hirai; R. Mori; H. Kikuta; N. Kato; K. Inoue; Y. Tanaka
    Digest of Papers - Microprocesses and Nanotechnology 1998: 1998 International Microprocesses and Nanotechnology Conference 1998-July 89 - 90 1998 [Refereed]
  • Yoshihiko Hirai; Ryosuke Mori; Hisao Kikuta; Nobuhiro Kato; Koji Inoue; Yoshio Tanaka
    Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers JAPAN J APPLIED PHYSICS 37 (12 B) 7064 - 7069 0021-4922 1998 [Refereed]
     
    The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also, the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results. © 1998 Publication Board, Japanese Journal of Applied Physics.
  • Toshiro Matsumoto; Nobuhiro Kato; Masao Taga
    Jpn. J. Al. Phys. 37 (9A) 5088 - 5093 0021-4922 1998
  • Jpn. J. Al. Phys. 37 (1) 9A,1169-1171  1998
  • Nobuhiro Kato; Ippei Suzuki; Hisao Kikuta; Koichi Iwata
    Review of Scientific Instruments AMER INST PHYSICS 68 (6) 2475 - 2478 0034-6748 1997/06 [Refereed]
     
    A microforce sensor with a force feedback method for scanning force microscopy is presented. The force sensor is constructed by using an optical fiber and a microcantilever. The facet of the optical fiber is coated with a gold thin film 15 Å thick. This gold film acts not only as a partially reflected mirror but also as an electrode of the electrostatic actuator. The interaction force between a probe tip and a sample is balanced by the electrostatic force. The deflection of the cantilever is measured by an interferometer consisting of the facet of the optical fiber and the cantilever surface. We have made the force sensor and measured some force curves of a mica surface with a SiN probe tip. The force curves obtained by this sensor are quite different from the curves by a conventional measurement without a feedback system. The force resolution of the system is 10-10 N with a bandwidth of dc∼1 kHz. © 1997 American Institute of Physics.
  • Toshiro Matsumoto Nobuhiro; Kato Kentaro Tamamura Arata; Kojima Noboru Izuchi
    Memoirs of the School of Biology-Oriented Science and Technology of Kinki University Kinki University 2 50 - 58 1342-7202 1997 [Refereed]
     
    The deformations of the lumbar vertebrae with and without interarticular part cut off artificially to simulate hemi-spondylolysis were measured to consider mechanical properties by the double exposure holographic interferometry. Following results were obtained. (1) In the case of the normal lumbar vertebra, displacement due to the load applied to the inferior articular process was more than that of superior articular process under the same load. (2) In the case of the lumbar vertebra with interarticular part cut off artificially, the interarticular part was subjected to the large stress. From these points, one of the valuable data to consider the cause of spondylolysis was obtained.
  • Matsumoto Toshiro; Kato Noboru; Tamamura Kentaro; Kojima Arata; Izuchi Noboru
    Memoirs of THE SCHOOL OF BIO-ORIENTED SCI. & TECHNOL. OF KINKI UNIV. Kinki University 2 (2) 50 - 58 1342-7202 1997 
    The deformations of the lumbar vertebrae with and without interarticular part cut off artificially to simulate hemi-spondylolysis were measured to consider mechanical properties by the double exposure holographic interferometry. Following results were obtained. (1) In the case of the normal lumbar vertebra, displacement due to the load applied to the inferior articular process was more than that of superior articular process under the same load. (2) In the case of the lumbar vertebra with interarticular part cut off artificially, the interarticular part was subjected to the large stress. From these points, one of the valuable data to consider the cause of spondylolysis was obtained.
  • Nobuhiro Kato; Ippei Suzuki; Hisao Kikuta; Koichi Iwata
    Review of Scientific Instruments AMER INST PHYSICS 66 (12) 5532 - 5536 0034-6748 1995 [Refereed]
     
    Scanning force microscopes (SFMs) are sometimes used to obtain a force curve, which shows the force variation as a function of tip-sample distance. In the force curve measurement, if the spring constant of the force detecting lever is small, the measured force curve has discontinuity and is different from the true force curve. In this paper, we present a new type of force balancing force sensor built in SFM. This force sensor employs an optical lever for detecting the rotation of the lever and two electrostatic force actuators with transparent electrodes. This sensor has two operating modes; with and without feedback. In the feedback mode, the force detecting lever is balanced with the electrostatic force. This system has the effect of enlarging the effective spring constant of the whole sensor. In the nonfeedback mode, this sensor acts as an ordinary force sensor. By using this sensor in both modes we will show the effectiveness of the force feedback in force curve measurements. © 1995 American Institute of Physics.
  • Hisao Kikuta; Kosuke Nasu; Nobuhiro Kato; Koichi Iwata
    Review of Scientific Instruments AMER INST PHYSICS 66 (1) 87 - 90 0034-6748 1995 [Refereed]
     
    An atomic force microscope has been constructed which enables us to observe sample surfaces through a metallurgical optical microscope. The cantilever deflection is detected by a polarization common-path interferometer with optical heterodyne technique. The optical system of the interferometer is incorporated in the commercial optical microscope. The common-path interferometer is rather insensitive to the effects of environmental disturbance such as thermal drift and mechanical vibration. The sample surface and the cantilever are observed by a microscopic objective of ×20. We can adjust the interferometer easily by observing both the cantilever and laser beams. The detection system has a total rms noise of 0.35 Å in a frequency bandwidth of 0.5-200 Hz on a free Si3N4 cantilever of 0.1 N/m spring constant. © 1995 American Institute of Physics.

MISC

  • Pro/ENGINEER for biginners
    藤井 雅雄; 加藤 暢宏; 田口 晋也; 清水  2005/05
  • ABC for Pro/ENGINEER
    藤井 雅雄; 加藤 暢宏; 田口 晋也; 清水  2004/11

Books and other publications

  • 加藤暢宏; 横山真央; 田中篤 (Contributor第８節 マイクロニードル技術の局所止血創面被覆材への応用（p308-p316))技術情報協会 2024/03 9784867980149 482p p308-p316
  • 加藤暢宏 (Contributor第7章 生体計測装置学 7-1)丸善出版 2024/01 9784621309018 9,210p
  • 次世代マイクロニードルの開発と応用
    加藤暢宏 (ContributorP42-P51)シーエムシー出版 2022/12 9784781317205
  • 加藤暢宏 (Joint workp65-p70)シーエムシー出版 2016 9784781311791
  • Advanced Technologies of Cell Separation and Manipulation
    加藤 暢宏; Gary D. Smith; Shuichi Takayama (Joint work第６章 マイクロフルイディクスによる細胞の分離と計測)シーエムシー出版 2008/04 9784781300047 
    本書は，急速に発展しつつある「細胞分離・操作技術」の最先端研究・開発について基礎から応用まで包括的な情報を提供することを目的としています。細胞分離・操作技術の基盤技術として細胞分離・操作に関する方法論と応用例を紹介しています。

Lectures, oral presentations, etc.

  • 研究室から臨床へ:基礎研究の成果と挑戦 副腎静脈サンプリングにおけるアルドステロン濃度の血流モデリング
    福田 耕大; 加藤 暢宏; 東野 信行; 小山 貴生; 生駒 顕; 南口 博紀; 河合 信行; 園村 哲郎
    日本インターベンショナルラジオロジー学会雑誌  2024/04  (一社)日本インターベンショナルラジオロジー学会
  • Nobuhiro Kato
    the 2nd. Korea-Japan Joint Workshop on MAP  2024/03
  • 有限要素法によるマイクロニードルメッシュシートの解析
    加藤暢宏; 吉川奏楽; 今村駿介
    精密工学会大会学術講演会講演論文集(CD-ROM)  2024
  • Shunsuke Imamura; Taiga Asami; Tomoya Akaki; Atsushi Tanaka; Kouhei Yamaji; Nobuhiro Kato
    7th International Conference on Microneedles (MICRONEEDLES 2023)  2023/05
  • 穿刺抵抗の低減を目的とした新規構造を有するマイクロニードルの提案とその力学的評価
    今村駿介; 定野和憲; 田中篤; 岡田瑶平; 伊藤巧真; 成田夏蓮; 中川花栞; 吉川奏楽; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2023
  • Stanford A型急性大動脈解離に対する人工血管置換術における解離腔補強のための人工中膜の開発
    西村好晴; 本田賢太朗; 國本秀樹; 藤本貴大; 生地みづ穂; 上松耕太; 田中篤; 加藤暢宏
    脈管学(Web)  2023
  • 基部構造の一部省略によるマイクロニードルメッシュシートの柔軟性の向上と評価
    今村駿介; 浅見泰雅; 赤木智哉; 田中篤; 山路宏平; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2023
  • 烏賊の吸盤を生体模倣したマイクロニードルメッシュシートの作製と評価
    浅見泰雅; 定野和憲; 本田賢太郎; 西村好晴; 田中篤; 高谷亮太; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2023
  • Taiga ASAMI; Tomoya AKAKI; Shunsuke IMAMURA; Kentaro HONDA; Yoshiharu NISHIMURA; Atsushi TANAKA; Nobuhiro KATO
    19th International Conference on Precision Engineering (ICPE2022)
  • Taiga Asami; Shunsuke Imamura; Tomoya Akaki; Kentaro Honda; Yoshiharu Nishimura; Atsushi Tanaka; Nobuhiro Kato
    the 9th Intl. Conf. of Asian Society for Precision Engg. and Nanotechnology (ASPEN 2022)
  • 赤木 智哉; 浅見 泰雅; 小田 慎一郎; 岩上 雄祐; 平井 慶充; 西村 好晴; 田中 篤; 加藤 暢宏
    精密工学会学術講演会講演論文集  2022/08  公益社団法人 精密工学会
     
    先行研究で体内における止血デバイスとして開発されたマイクロニードルシート（MNS）は肺気胸の手術デバイスとして活用できる可能性が示唆されている。しかし、湿潤環境である胸腔内では表面張力によるMNSの初期接着は容易ではない。また、止血用のMNSの基部は格子状であり、隙間から空気漏れを起こす可能性が高い。本研究では、接着性を示す薬剤をMNS全体に塗布することで、肺瘻の閉塞に適したMNSの作製を試みた。
  • 浅見 泰雅; 草開 義希; 今村 駿介; 赤木 智哉; 本田 賢太郎; 西村 好晴; 田中 篤; 加藤 暢宏
    精密工学会学術講演会講演論文集  2022/08  公益社団法人 精密工学会
     
    心臓外科手術において、縫合が難しい創には局所創面被覆剤としてフィブリン糊などによる止血が行われる。フィブリンは血液製剤であり感染症のリスク要因となるため使用量の削減が望まれている。本研究ではフィブリン糊の削減を目指して局所創面被覆剤として用いることができるマイクロニードルシートを提案する。具体的には、心臓の伸縮に追従し、なおかつ高い組織接着力を有するマイクロニードルメッシュの作製と評価を行った。
  • 基部に柔軟性と等方性を有するマイクロニードルパッチの作製と評価
    今村駿介; 浅見泰雅; 赤木智哉; 田中篤; 山路宏平; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2022
  • 面内方向の伸縮性を有するマイクロニードルメッシュの作製と評価
    浅見泰雅; 田中篤; 堺谷泰成; 中西樹; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2022
  • 500μm以上の高さを有するマイクロニードルの作製
    赤木智哉; 酒井啓介; 浅見泰雅; 田中篤; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2022
  • 横山真央; 田中篤; 加藤暢宏
    第58回日本リハビリテーション医学会学術集会  2021/06
  • マイクロニードル技術を利用した新しい褥瘡面の止血治療法
    横山 真央; 田中 篤; 加藤 暢宏
    The Japanese Journal of Rehabilitation Medicine  2021/05  (公社)日本リハビリテーション医学会
  • 医工連携によるマイクロニードルシートを用いた新規肺瘻閉鎖シート開発の試み
    平井慶充; 加藤暢宏; 大橋拓矢; 矢田由美; 房本安矢; 井口豪人; 宮坂美和子; 川路万理; 西村好晴
    日本呼吸器外科学会総会(Web)  2021/05
  • Establishment of ZIKVE protein-expressing yeast strain for development of oral mucosal vaccine
    坂口久峻; 辻彩花; 東慶直; 正木秀幸; 加藤暢宏
    日本生体医工学会大会プログラム・抄録集(Web)  2021
  • Effect of Buffer Layers for Transfer Process of Anatase Nb: TiO2 Epitaxial Thin Films to Flexible Sheets Using Water-soluble Sacrificial Layer
    平岡壮大; 加藤暢宏; 藤原宏平; 西川博昭
    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2021
  • 卵円孔開存の新規治療デバイスとしてのマイクロニードルパッチの開発
    浅見泰雅; 田中篤; 中西樹; 堺谷泰成; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2021
  • 手術部マーカーとしてのマイクロニードルシートの作製
    赤木智哉; 森本大貴; 今村駿介; 田中篤; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2021
  • 加藤 暢宏; 千原 なみえ; 横山 真央; 田中 篤; 赤阪 隆史
    精密工学会学術講演会講演論文集  2020  公益社団法人 精密工学会
     

    手術用止血用シートには血液製剤が用いられることが多い。止血性能の向上、費用の抑制、感染症リスクの低減などが求められている。本研究ではフォトリソグラフィにより作製したマイクロニードルを原型に、熱インプリントにより作製したポリ乳酸製マイクロニードルメッシュで非生物由来の高性能止血シートの実現を目指している。生体組織への穿刺および止血機序について観察・評価した結果を報告する。

  • ヒト間葉系幹細胞から軟骨細胞への分化誘導を促進する細胞低接着性コラーゲン
    國井 沙織; 加藤 暢宏; 山本 衛; 伊田 寛之; 平岡 陽介; 森本 康一
    日本バイオマテリアル学会大会予稿集  2019/11  日本バイオマテリアル学会
  • 細胞低接着性コラーゲンにより制御される骨芽細胞への分子誘導遺伝子群の解析
    國井 沙織; 山本 衛; 加藤 暢宏; 伊田 寛之; 平岡 陽介; 森本 康一
    日本バイオマテリアル学会大会予稿集  2019/11  日本バイオマテリアル学会
  • Occlusion of dental tubule with amorphous phosphate sheet prepared by KrF pulsed laser deposition method
    Nobuhiro Kato; Mizuki Hirai; Tomoki Minamino; Kazushi Yoshikawa; Shigeki Hontsu
    The First Asian Pacific Division of the World Federation for Laser Dentistry Conference 2019  2019/11
  • Repair of enamel pits using Er:YAG laser film deposition method
    Tomoki Minamino; Ei Yamamoto; Nobuhiro Kato; Hiroaki Nishikawa; Shigeki Hontsu
    The First Asian Pacific Division of the World Federation for Laser Dentistry Conference 2019  2019/11
  • 高橋 鷹兵; 根本 充貴; 加藤 暢宏; 朴 忠植; 山東 悠介; 佐伯 和弘
    システム制御情報学会研究発表講演会講演論文集  2019/05
  • 千原 なみえ; 坂本 佳奈子; 横山 真央; 樽谷 玲; 田中 篤; 赤阪 隆史; 加藤 暢宏
    精密工学会学術講演会講演論文集  2019  公益社団法人 精密工学会
     

    外科手術において縫合が難しい創が生じた際は局所止血用創面被覆材を用いた止血が行われる。既存の被覆材には生体由来材料が使われており感染等の危険がある。我々は新規の被覆材としてメッシュ状の基部を有するポリ乳酸製マイクロニードル(MN)パッチを提案してきた。本研究ではマウスの肝臓に作った創にMNパッチを貼付し、止血効果を確認した。その結果、創は数秒で止血され、留置1ヵ月後において炎症などの症状は見られなかった。

  • 森中 杏菜; 和澤 充宏; 坂本 佳奈子; 植田 留名; 千原 なみえ; 正木 秀幸; 加藤 暢宏
    精密工学会学術講演会講演論文集  2019  公益社団法人 精密工学会
     

    先行研究では蛍光顕微鏡やOCTを用いてマイクロニードル(ＭＮ)を穿刺した皮膚の断層像の撮像や穿刺前と後のＭＮの高さを比較することでＭＮの溶解の観察を行っていた。これらの方法では立体的に観察ができず、同一のＭＮによる経時的な観察を行うことができない。そこでマイクロＣＴを用いて撮像を行い、立体的かつ経時的なＭＮの溶解の様子を観察した。結果よりＭＮの溶解に適した貼付時間を決定した。

  • 坂本 佳奈子; 繁田 一穂; 中尾 紗貴; 千原 なみえ; 森中 杏菜; 植田 留名; 加藤 暢宏
    精密工学会学術講演会講演論文集  2019  公益社団法人 精密工学会
     

    先行研究では移動マスク露光と複数波長逐次露光の併用により作製したマイクロニードル(ＭＮ)の先端部が湾曲する現象が確認された。この現象の原因はレジストの強度不足であると推測される。本研究では露光波長と露光量を変更してレジストの露光を行い、各条件下での硬度をマイクロビッカース試験機で測定した。露光量、紫外線波長と硬度の関係を明らかにすることで、ＭＮが湾曲しにくい作製条件を検討した。

  • 植田 留名; 西田 速人; 坂本 佳奈子; 千原 なみえ; 森中 杏菜; 加藤 暢宏
    精密工学会学術講演会講演論文集  2019  公益社団法人 精密工学会
     

    化粧品のテストなどに供される人工皮膚には、角質層、表皮層などの構造を有するものは知られていない。これらをマイクロニードル(MN)の穿刺実験に用いると、皮膚のごく浅い部分でのMNの挙動は評価できない。本研究では、MNの穿刺実験の定量評価を目的として人工皮膚を開発した。PDMSとポリウレタンを用いて作製した人工皮膚と人間の皮膚の力学特性を比較した結果、作成した人工皮膚は実際の皮膚に近い力学特性を持つことが分かった。

  • Stability of ultrathin amorphous calcium phosphate artificial enamel sheet sheets under simulated intraoral environment condition
    Nobuhiro Kato; Mizuki Hirai; Ei Yamamoto; Shigeki Hontsu
    18 th Asian Bioceramics Symposium；ABC2018  2018/09
  • マイクロニードルにおける薬剤の配置制御  [Not invited]
    森中杏菜; 坂本佳奈子; 植田留名; 千原なみえ; 加藤暢宏
    生体医工学シンポジウム2018  2018/09
  • マイクロローラーポンプによって駆動される灌流型細胞培養デバイス  [Not invited]
    植田留名; 千原なみえ; 森中杏菜; 坂本佳奈子; 加藤暢宏
    生体医工学シンポジウム2018  2018/09
  • 千原なみえ; 坂本佳奈子; 森中杏菜; 植田留名; 萬屋沙織; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2018/08 

    従来のマイクロニードル(MN)パッチは、柔軟性に問題があった。我々はこれまでに、3Dプリンタを用いてポリ乳酸(PLA)をメッシュ状に成形し、MNを熱インプリントすることで柔軟なMNパッチを作成してきた。本研究ではメッシュ状に成形したPLAシートの柔軟性を定量的に評価する方法を提案し、測定を行った。その結果MNパッチの柔軟性は、メッシュを構成する線材の太さに影響を受けることが分かった。

  • 坂本佳奈子; 千原なみえ; 森中杏菜; 植田留名; 加藤暢宏
    精密工学会大会学術講演会講演論文集  2018/08 

    熱インプリントによりメッシュ状マイクロニードルパッチを作製する際に、メッシュ部分の形状が一定しないという問題があった。本研究では移動マスク露光と複数波長逐次露光を併用することで、メッシュ部分の形状の維持を目的としたメッシュ状支持部を持ったフォトレジスト製のマイクロニードルを作製した。

  • Microneedles fabricated by heat imprinting for novel application  [Invited]
    KATO Nobuhiro
    Microneedles2018  2018/05  Vancouver, Canada
  • エナメル質修復用フレキシブル非晶質リン酸カルシウムシートの長期安定性
    平井瑞樹; 南野智紀; 山本衛; 加藤暢宏; 本津茂樹
    バイオインテグレーション学会第８回総会・学術大会  2018/05
  • 脱灰・再石灰化環境内での極薄非晶質リン酸カルシウム人工エナメル質シートの安定性  [Not invited]
    平井 瑞樹; 本津 茂樹; 山本 衛; 加藤 暢宏
    日本歯科理工学会誌  2018/03
  • 加藤暢宏; 千原なみえ; 坂本佳奈子; 森中杏菜
    精密工学会大会学術講演会講演論文集  2018/03 

    移動マスク露光法により得られたマイクロニードルのフォトレジスト型を原型に、シリコーンエラストマーを用いてレプリカ型を得た。このレプリカ型に対して生体適合性のある熱可塑性樹脂を熱インプリントすることでマイクロニードルアレイを得ることができた。熱インプリントに先立って熱可塑性樹脂をあらかじめ所望の形状に整形しておくことでマイクロニードルアレイの機械的特性を制御できることが示唆された。

  • 森本康一; 國井沙織; 武岡由樹; 由留部崇; 加藤暢宏; 尾前薫; 黒田良祐
    日本バイオマテリアル学会大会予稿集(Web)  2018
  • 國井沙織; 兼清健志; 中野法彦; 加藤暢宏; 尾前薫; 井出千束; 森本康一
    日本バイオマテリアル学会大会予稿集(Web)  2018
  • 森本康一; 國井沙織; 伊田寛之; 平岡陽介; 加藤暢宏
    日本バイオマテリアル学会大会予稿集(Web)  2017/11
  • Development of Ultra-Thin Opaque White Hydroxyapatite Sheet for Restoration of Enamel and Aesthetic Treatments of Teeth  [Not invited]
    Nobuhiro Kato; Yuki Ido; Ei Yamamoto; Shigeki Hontsu
    Bioceramics 29  2017/10  Toulouse, France
  • Adhesive Evaluation by Brushing Tests for Hydroxyapatite Films Fabricated on Dentins Using a Water Mist Assisted Er:YAG Laser Deposition Method  [Not invited]
    Ei Yamamoto; Nobuhiro Kato; Shigeki Hontsu
    Bioceramics 29  2017/10  Toulouse, France
  • 平井瑞樹; 本津茂樹; 片山一郎; 加藤暢宏; 橋本典也; 吉川一志; 山本一世
    日本歯科理工学会誌  2017/09
  • KATO Nobuhiro; SAKAMOTO Kanako; CHIHARA Namie; MORINAKA Anna
    精密工学会学術講演会講演論文集  2017/09 
    フォトリソグラフィを用いてマイクロニードルを作成する場合、フォトレジストの膜厚が非常に大きくなるため、レジストのコーティングおよび現像に注意が必要である。本研究ではディスペンサロボットを用いて基板上の任意の位置に膜厚500μm以上でレジストをコートする方法、ならびにリングポンプを用いた現像状態をその場観察可能な灌流現像システムを開発した。
  • KATO Nobuhiro; MORI Shunta
    Proceedings of JSPE Semestrial Meeting  2017/03 
    透明基板裏面からのネガ型厚膜フォトレジストへの移動マスク露光によってマイクロニードルの形成が可能である。従来は単一波長(λ=365nm)の紫外線のみを使用しており、形成できる形状に制約があった。本研究ではフォトレジストの吸光特性の測定結果に基づいて、通常は露光に使用されない複数波長の紫外線を使用することでニードル部分と土台部分の形状の自由度を飛躍的に向上させた。
  • 本津茂樹; 山本衛; 加藤暢宏; 保尾謙三; 吉川一志; 山本一世
    日本レーザー歯学会総会・学術大会プログラム・講演抄録集  2016/12
  • Optimization of Humid Conditions Using an Ultrasonic Nebulizer for the Fabrication of Hydroxyapatite Film with the Er:YAG Laser Deposition Method  [Not invited]
    Ei Yamamoto; Nobuhiro Kato; Yuka Hatoko; Shigeki Hontsu
    Bioceramics 28  2016/11  Charlotte, NC, USA
  • Preparation and Application of a Potassium-Substituted Calcium Phosphate Sheet as a Dental Material for Treating Dentin Hypersensitivity  [Not invited]
    Kato, N; Y. Hatoko; E. Yamamoto; T. Furuzono; S. Hontsu
    Bioceramics 28  2016/11  Charlotte, NC, USA
  • 國井沙織; 加藤暢宏; 堀内喜高; 森本康一
    日本生化学会大会(Web)  2016/09
  • Arbitrary shape microneedle generation using moving-mask lithography  [Not invited]
    Nobuhiro Kato; Shunta Mori
    Microneedles 2016  2016/05
  • 井戸雄基; 山本衛; 加藤暢宏; 保尾謙三; 吉川一志; 山本一世; 本津茂樹
    日本歯科理工学会誌  2016/03
  • 小比賀優; 波床侑果; 加藤暢宏; 山本衛; 吉川一志; 山本一世; 本津茂樹
    日本歯科理工学会誌  2016/03
  • 本津茂樹; 波床侑果; 山本衛; 加藤暢宏; 吉川一志; 山本一世
    日本歯科理工学会誌  2016/03
  • 藤木雄太; 加藤暢宏; 宮田静; 朴忠植; 山東悠介; 佐伯和弘
    日本はい移植学雑誌  2016/01
  • Kadota Takayuki; Katou Nobuhiro
    Transactions of Japanese Society for Medical and Biological Engineering  2016 

    Flow rate of fluid in micro channel driven by gravity pump is proportional to the head difference. Regulator for long-term microfluidic cell culture system was needed. The conventional flow regulator systems enabling constant flow rate are not easy to miniaturize to fit on micro channels. In this study, simple passive regulator systems that can be applied to micro channel are presented. The result of FEM analysis predicted the effectiveness of deformable valve structure. The analyzed results were evaluated by experiment using microfluidic system with 4 types deformable passive valves.

  • Mori Shunta; Kai Takahisa; Susaki Yusuke; Yamamoto Ei; Kato Nobuhiro
    Transactions of Japanese Society for Medical and Biological Engineering  2016 

    Microneedles (MNs) are transdermal drug delivery systems, making it possible to administer drugs into the dermis without pain. In this study, master male molds of MNs were obtained by moving-mask UV lithography. The MNs female molds were replicated by poly-dimethylsiloxane. Finally, MNs were obtained by casting chondroitin sulfate C sodium salt (CSC) solution to the female molds. The Young's modulus of CSC was evaluated to be 2-4 GPa by tensile test. The CSC was enough stiff to penetrate epidermis. The penetration tests were conducted to characterize various shapes of CSC-MNs.

  • Microneedles Protoryping Using Moving-Mask Lithography  [Not invited]
    Nobuhiro Kato; Takahisa Kai; Shunta Mori
    12th International Conference on Ubiquitous Healthcare  2015/12
  • Adhesion Properties of an Apatite Film Deposited on Dentine Using Er:YAG Laser Ablation Methods  [Not invited]
    Ei YAMAMOTO Nobuhiro; KATO Kazushi YOSHIKAWA Kenzo; YASUO Kazuyo; YAMAMOTO Shigeki HONTSU
    Bioceramics 27  2015/10
  • Overlaid Ultrathin Amorphous Calcium Phosphate Sheet Improves Dentinal Permeability Inhibition Rate  [Not invited]
    Nobuhiro Kato; Yuki Ido; Ei Yamamoto; Kenzo Yasuo; Kazushi Yoshikawa; Shigeki Hontsu
    Bioceramics 27  2015/10
  • NISHI Shingo; SUSAKI Yusuke; KAI Takahisa; MIYATA Shizuka; NISHIMOTO Masaya; TAKEMORI Kumiko; KATO Nobuhiro; ITO Hiroyuki; YAMAMOTO Ei
    Proceedings of the ... JSME Conference on Frontiers in Bioengineering  2015/10
  • Microneedles fabrication utilizing a moving-mask lithography  [Not invited]
    Tkahisa KAI; Shunta MORI; Nobuhiro KATO
    生体医工学シンポジウム2015  2015/09
  • Development of embryo monitoring system using time lapse cinematography  [Not invited]
    Shizuoka MIYATA; Choongsik PARK; Yusuke SANDO; Yuta FUJIKI; Kazuhiro SAEKI; Nobuhiro KATO
    生体医工学シンポジウム2015  2015/09
  • マイクロニードル型作製のための移動マスク露光法の拡張  [Not invited]
    森 駿太; 甲斐 貴久; 加藤 暢宏
    生体医工学シンポジウム2015  2015/09
  • 微小流路デバイスのための受動的レギュレータの提案とその有限要素法による解析  [Not invited]
    門田 貴之; 加藤 暢宏
    生体医工学シンポジウム2015  2015/09
  • Hatoko Y; Yamamoto E; Kato N; Nishikawa H; Yoshikawa K; Furuzono T; Yamamoto K; Hontsu S
    The journal of the Japanese Society for Dental Materials and Devices  2015/09 
    In the therapy for hypersensitivity of tooth, it is known that potassium (K) compounds are often added in the toothpaste because it is believed that neurotransmission is blocked by potassium ions. Recently, we have been proposing the attachment therapy of a hydroxyapatite (HAp) sheet on dentin for hyperesthesia. It is thought that addition of K ions in HAp sheet is more effective for relieve pain of tooth hypersensitivity. In this study, we prepared K-HAp thin film and evaluated it. K-HAp sheet was prepared by using a process for preparing thin film. After that, we evaluated the interface between K-HAp sheet and dentin.
  • Er:YAGレーザーアブレーション法で象牙質上に形成したアパタイト膜の固着特性  [Not invited]
    本津 茂樹; 山本 衛; 加藤 暢宏; 保尾 謙三; 吉川 一志; 山本 一世
    日本レーザー歯学会誌  2015/08
  • HONTSU SHIGEKI; IDO YUKI; YAMAMOTO EI; KATO NOBUHIRO; YASUO KENZO; YOSHIKAWA KAZUSHI; YAMAMOTO KAZUYO
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)  2015/06
  • MIYATA SHIZUKA; PARK CHOONGSIK; SANDO YUSUKE; SAEKI KAZUHIRO; KATO NOBUHIRO
    Transactions of Japanese Society for Medical and Biological Engineering  2015/05 
    A simple time-lapse cinematography device that provides useful insight for embryo quality scoring by series observation of embryos during culture was developed. The device consists of a microscope system, a PC controlled camera and an illumination system. All components are implemented in a waterproof enclosure to be operated inside a CO2 incubator. Operations for image capture including focus adjustment and illumination adjustment can be remotely performed using PC without removing embryos from the optimal gas and temperature conditions. Using this device, clear images including several embryos were captured and the cleavage timing of embryos can be exactly identified.
  • Ido Y; Isai A; Yamamoto E; Kato N; Nishikawa H; Yasuo K; Hashimoto Y; Yoshikawa K; Yamamoto K; Hontsu S
    The journal of the Japanese Society for Dental Materials and Devices  2015/03 
    We propose a new method to shield dentinal tubules by direct attaching an amorphous calcium phosphate (ACP) sheet on the dentin as a treatment of the hyperaesthesia. In this study, we fabricated the perforated ACP sheet in order not only to easily remove liquid on the dentinal surface but also to promote the remineralization at the interface between the sheet and dentin. The ACP sheet was prepared by the pulsed laser deposition technique and the laser process using a metal mask. The interface structure between the ACP sheet and dentin was observed by a SEM. In addition, using Pashle's method, we evaluated the inhibition effect of lap-strake perforated ACP sheets attached to the dentin on its permeability.
  • TANIGUCHI SHUNJI; TAKADA HIROTATSU; TARUMOTO HIDEYUKI; KATO NOBUHIRO; SAEKI KAZUHIRO
    日本はい移植学雑誌  2015/01  日本胚移植研究会
  • GnRH-FSH処理が黒毛和種雌牛のOPUに及ぼす影響
    谷口 俊仁; 高田 広達; 樽本 英幸; 加藤 暢宏; 佐伯 和弘
    日本胚移植学雑誌  2015/01  日本胚移植技術研究会
  • KAI TAKAHISA; MORI SHUNTA; KATO NOBUHIRO
    Transactions of Japanese Society for Medical and Biological Engineering  2015 
    A moving-mask exposure apparatus that allows backside exposure through transparent substrate to form the mold of microneedle patch with thick photoresist was developed. By choosing moving trajectory, various shape microneedle can be produced from a single mask pattern. The photoresist microneedle pattern was replicated by poly-dimethylsiloxane (PDMS). The mixture of chondroitin sulfate C and sterilized water was poured over the PDMS mold. Finally, the cured chondroitin sulfate C microneedle patch was detached from the PDMS mold. From scanning microscope observation, successful shape transfer form the photoresist MN to the chondroitin sulfate C microneedle was confirmed.
  • Evaluation of Dentin Tubule Sealing Rate Improved by Attaching Ultrathin Amorphous Calcium Phosphate Sheet  [Not invited]
    Kato; Nobuhiro Isai; Arata Yamamoto; Ei Nishikawa; Hiroaki Kusunoki; Masanobu Yoshikawa; Kazushi Yasuo; Kenzo Yamamoto; Kazuyo Hontsu; Shigeki
    Bioceramics 26  2014/11
  • A Novel Treatment for Dentine Cavities with Intraoral Laser Ablation Method Using an Er:YAG Laser  [Not invited]
    Ei Yamamoto; Nobuhiro Kato; Arata Isai; Hiroaki Nishikawa; Yoshiya Hashimoto; Kazushi Yoshikawa; Shigeki Hontsu
    Bioceramics 26  2014/11
  • KAI TAKAHISA; HIRANO MASAKAZU; KATO NOBUHIRO
    日本機械学会マイクロ・ナノ工学シンポジウム講演論文集(CD-ROM)  2014/10
  • NISHI Shingo; SUSAKI Yusuke; MIYATA Shizuka; TAKEDA Ippei; TAKEMORI Kumiko; KATO Nobuhiro; ITO Hiroyuki; YAMAMOTO Ei
    Proceedings of the ... JSME Conference on Frontiers in Bioengineering  2014/10
  • HONTSU SHIGEKI; YAMAMOTO EI; KATO NOBUHIRO; NISHIKAWA HIROAKI; YASUO KENZO; YOSHIKAWA KAZUSHI; YAMAMOTO KAZUYO
    日本歯科保存学会学術大会プログラムおよび講演抄録集(Web)  2014/10
  • 培養細胞常時観察装置の開発  [Not invited]
    宮田 静; 朴 忠植; 山東 悠介; 佐伯 和弘; 加藤 暢宏
    生体医工学シンポジウム2014  2014/09
  • 移動マスク露光法によるマイクロニードル型の作製  [Not invited]
    甲斐 貴久; 平野 雅一; 加藤 暢宏
    生体医工学シンポジウム2014  2014/09
  • Hatoko Y; Yamamoto E; Kato N; Nishikawa H; Yoshikawa K; Furuzono T; Yamamoto K; Hontsu S
    The journal of the Japanese Society for Dental Materials and Devices  2014/09 
    In toothpaste formulated for hypersensitivity, potassium compounds such as potassium nitrate and potassium bromide are often added because it is believed that neurotransmission is blocked by the function of potassium ions. Thus far, we have proposed attaching a hydroxyapatite sheet on dentin as a treatment for hyperesthesia. We think that it may be possible to block dental tubules and relieve pain by adding potassium to the sheet for sustained release. In this study, we prepared and evaluated a potassium-doped hydroxyapatite thin film, and applied the process to the preparation of a potassium-doped hydroxyapatite sheet.
  • Isai A; Yamamoto E; Kato N; Nishikawa H; Yasuo K; Hashimoto Y; Yoshikawa K; Yamamoto K; Hontsu S
    The journal of the Japanese Society for Dental Materials and Devices  2014/09 
    We have fabricated a flexible hydroxyapatite (HAp) sheet using pulsed laser deposition and a sheet isolation technique. We aim to form an artificial enamel layer on the dentin by an amorphous calcium phosphate (ACP) sheet with solubility higher than compared that of the crystallized sheet. In this study, using Pashle's method, we evaluated of the dentin permeability inhibition effect by double layer method of the ACP sheet on dentin as a cure for dental hypersensitivity. The dentin permeability inhibition rate of the dentinal tubule by double layer method of the ACP sheet was 89.17±7.24%. The ACP sheet was yielded results better than of dental resin cement.
  • 多層非晶質リン酸カルシウムシート貼付法による象牙質透過抑制率効果の評価  [Not invited]
    以西 新; 山本 衛; 加藤 暢宏; 西川 博昭; 保尾 謙三; 橋本 典也; 吉川 一志; 山本 一世; 本津 茂樹
    日本歯科理工学会誌  2014/09
  • 極薄カリウム含有ハイドロキシアパタイトシートの作製と評価  [Not invited]
    波床 侑果; 山本 衛; 加藤 暢宏; 西川 博昭; 吉川 一志; 古薗 勉; 山本 一世; 本津 茂樹
    日本歯科理工学会誌  2014/09
  • HATOKO YUKA; YAMAMOTO EI; KATO NOBUHIRO; NISHIKAWA HIROAKI; FURUZONO TSUTOMU; HONTSU SHIGEKI
    日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM)  2014/08
  • Moving-mask Lithography for 3D Microstructure Molding  [Not invited]
    Nobuhiro Kato; Takahisa Kai; Masakazu Hirano
    ICPST-31  2014/07
  • Er:YAGレーザー成膜法による歯質上へのアパタイト膜の直接形成  [Not invited]
    本津 茂樹; 山本 衛; 加藤 暢宏; 西川 博昭; 吉川 一志; 山本 一世
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2014/06
  • Hontsu S; Yamamoto E; Kato N; Nishikawa H; Hashimoto Y; Yoshikawa K; Yamamoto K
    The journal of the Japanese Society for Dental Materials and Devices  2014/03 
    A calcium phosphate film was deposited on the dentine in the air by applying Er:YAG laser to the laser ablation (PLD) method to try the restoration of the dentine. α-tricalcium phosphate (α-TCP) was used as a target for the film preparation, and cattle enamel and human dentin were used as a substrate. X-ray diffraction (XRD) technique was used for crystallinity evaluation of the deposited layer, and scanning electron microscope (SEM) was used for the evaluation of the substrate-deposited layer interface.
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養  [Not invited]
    岩本 太作; 谷口 俊仁; 岸 昌生; 加藤 暢宏; 佐伯 和弘
    日本胚移植学雑誌  2014/01
  • 移動マスク露光によるマイクロニードル型の作成  [Not invited]
    加藤暢宏; 甲斐貴久; 平野雅一
    化学とマイクロ・ナノシステム学会 第28回研究会  2013/12
  • 極薄アパタイトシートを用いた象牙細管の封鎖効果についての検討(第2報)  [Not invited]
    本津 茂樹; 以西 新; 山本 衛; 加藤 暢宏; 西川 博昭; 吉川 一志; 山本 一世
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2013/10
  • 移動マスクUV露光による2.5次元形状の生成  [Not invited]
    加藤暢宏; シャルマ・マークン; 前坂圭亮; 平野雅一
    日本機械学会2013年度年次大会  2013/09
  • 象牙質—極薄アパタイトシート界面における固着特性の評価  [Not invited]
    以西新; 松本明子; 山本衛; 加藤暢宏; 西川; 博昭 楠正暢; 本津茂樹; 吉川一志
    日本セラミックス協会第26 回秋季シンポジウム講演予稿集(CD−ROM)|L2240B  2013/09
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養  [Not invited]
    谷口俊仁; 岩本太作; 岸昌生; 加藤暢宏; 佐伯和弘
    平成25年度近畿地区連合獣医師大会・獣医学術近畿地区学会  2013/09
  • PDMSマイクロウェルを用いたウシ卵子の成熟培養  [Not invited]
    岩本太作; 谷口俊仁; 岸昌生; 加藤暢宏; 佐伯和弘; 谷口俊仁
    第20回日本胚移植研究会  2013/08
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養  [Not invited]
    岩本太作; 谷口俊仁; 岸昌生; 加藤暢宏; 佐伯和弘; 谷口俊仁
    東日本家畜受精卵移植技術研究会大会  2013/08
  • 極薄アパタイトシートを用いた象牙細管の封鎖効果についての検討  [Not invited]
    以西 新; 山本 衛; 加藤 暢宏; 西川 博昭; 吉川 一志; 山本 一世; 本津 茂樹
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2013/05
  • Hontsu S; Yoshikawa K; Isai A; Yamamoto E; Kato N; Nishikawa H; Kusunoki M; Hashimoto Y; Yamamoto K
    The journal of the Japanese Society for Dental Materials and Devices  2013/03 
    We have successfully created a flexible hydroxyapatite (HAp) sheet of less than a few micrometers, and we have been trying to establish the all-HAp restoration technique for tooth enamel. In this study, we evaluated the re-calcification layer at the various sheets (amorphous HAp, HAp, amorphous HAp/amorphous calcium phosphate: ACP, HAp/ACP)-enamel interface by scanning electron microscope (SEM).
  • エナメル質-極薄アパタイトシート界面における再石灰化層の検討  [Not invited]
    本津茂樹; 以西新; 加藤暢宏; 山本衛; 西川博昭; 楠正暢; 吉川一志
    第7回ナノ・バイオメディカル学会大会  2013/01
  • KATO Nobuhiro; SHARMA Markun; MAESAKA Keisuke; HIRANO Masakazu
    The Proceedings of Mechanical Engineering Congress, Japan  2013  The Japan Society of Mechanical Engineers
     
    A novel moving mask exposure apparatus for 2.5 dimensional patterning of thick photo resist has been developed. In general mask aligner, the UV light intensity that passes through photo mask shows exactly same shape of mask pattern. By in plane moving PZT-stage which carrying photoresist coated substrate, the UV intensity distribution via photo mask has been modified from simple binary pattern to Gaussian like pattern. The stage movement can be controlled by applying signal to stage that enables to generate arbitrary UV intensity distribution. Using the exposure apparatus and backside exposure technique we created photoresist molds for micro well array that have smoothly tilted sidewall. These photo resist molds were replicated by Poly-(dimethylpolysiloxane) to make final product with good shape transferability.
  • 穴あきアパタイトシートを用いたエナメル質の修復  [Not invited]
    本津 茂樹; 以西 新; 山本 衛; 加藤 暢宏; 西川 博昭; 楠 正暢; 速水 尚; 吉川 一志
    日本バイオマテリアル学会大会予稿集  2012/11
  • Adhesive Strength Between Flexible Hydroxyapatite Sheet and Tooth Enamel  [Not invited]
    E. Yamamoto; N. Kato; H. Nishikawa M; Kusunoki T; Hayami S. Hontsu
    Bioceramics 24  2012/10
  • 穴あき極薄アパタイトシートによるエナメル質の修復  [Not invited]
    本津 茂樹; 以西 新; 山本 衛; 加藤 暢宏; 西川 博昭; 吉川 一志; 山本 一世
    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2012/10
  • Hontsu S; Yoshikawa K; Isai A; Yamamoto E; Kato N; Nishikawa H; Kusunoki M; Hashimoto Y; Yamamoto K
    The journal of the Japanese Society for Dental Materials and Devices  2012/09 
    We have fabricated a flexible hydroxyapatite (HAp) using a pulsed laser deposition (PLD) method and a film isolation technique. We adhered the HAp sheet to the dentine surface using a calcium phosphate aqueous in order to apply this sheet to hyperesthetic treatment. In the present study, the time dependence of the composition at the interfaces between the HAp/TCP sheet and the dentine was evaluated using X-ray diffraction measurement, and the adhesive strength between the HAp/TCP sheet and the dentine surface was evaluated by quasi-static tensile tests.
  • Matsubara Atsushi; Kogiso Nozomu; Kato Nobuhiro; Yokokoji Yasuyoshi; Tutsui Shinsaku; Makino Osamu
    Mechanical Engineering Congress, Japan  2012/09 
    An education method for mechanical design is discussed. In conventional design education, the prototype designs together with design process are given for students, which can be referred to as parameterized design. This is a good introduction for students to start the design class. However, the big picture of the design process could not be learned. For students to experience total design paradigm, a design seminar has been opened every summer in past decade. We discuss the pros and cons of the current seminar and present the perspective of next turn.
  • Hontsu S; Yoshikawa K; Matsuda T; Kato N; Nishikawa H; Kusunoki M; Hashimoto Y; Yamamoto K
    The journal of the Japanese Society for Dental Materials and Devices  2012/03 
    We have succeeded to fabricate flexible hydroxyapatite (HAp) sheet for adhering directly onto tooth and bone surfaces using a pulsed laser deposition method and a film isolation technique. We have investigated a method to regenerate the tooth enamel by just attaching the flexible HAp sheet onto the tooth surface with artificial saliva. In this paper, we report the result of adhering the HAp sheet to the enamel assisted by an TCP over-deposition and calcium phosphate aqueous solution in order to reduce the period required to regenerate the enamel.
  • フレキシブル極薄フッ素化アパタイトシートの作製  [Not invited]
    本津 茂樹; 丸山 敏朗; 加藤 暢宏; 西川 博昭; 楠 正暢; 速水 尚; 橋本 典也
    日本バイオマテリアル学会大会予稿集  2011/11
  • シリコーン製マイクロウェルの容積と形状がウシ胚の初期発生に及ぼす影響  [Not invited]
    庄 隼生; 岩本 太作; 加藤 暢宏; 河上 泰之; 谷口 俊仁; 岸 昌生; 細井 美彦; 松本 和也; 入谷 明; 佐伯 和弘
    日本胚移植学雑誌  2011/01
  • The restoration of dental enamel using the flexible apatite sheet  [Not invited]
    本津 茂樹; 加藤 暢宏; 速水 尚; 西川 博昭; 楠 正暢; 吉川 一志; 山本
    5th International Symposium on Apatites and Correlative Biomaterials  2010/12  オーストラリア（ケアンズ）  5th International Symposium on Apatites and Correlative Biomaterials
  • 森本 康一; 西山 裕之; 國井 沙織; 角田 昌明; 加藤 暢宏
    Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要  2010/09 
    灌流培養環境下でHL60細胞とI型コラーゲンとの相互作用を観察するため, poly (dimethylsiloxane)を用いて微小流路デバイス(幅700μm×高さ100μm×長さ20mm)を作製した. 矩形断面を有する微小流路内壁4面すべてを酸素プラズマ処理し, ニワトリI型コラーゲンとウシ胎児血清成分タンパク質を灌流して固定化した. 固定化されたタンパク質の吸着量をSDS-ポリアクリルアミドゲル電気泳動にて定量的に調べた結果, 血清タンパク質に対して酸素プラズマ処理の効果は認められなかったが, I型コラーゲンでは酸素プラズマ処理の効果が経時的に減少するに従い, その固定化量が増加した. 作製した微小流路デバイスを用いた0.2μL/minの灌流培養下で, HL60細胞はI型コラーゲンにのみローリングあるいは接着挙動を示した. 酸素プラズマ処理の効果はI型コラーゲンに対する細胞接着に大きく影響した. HL60細胞の接着挙動は固定化されたI型コラーゲン量に単純に比例せず, その構造変化を認識することが示唆された. (英文) We developed microfluidic device (W 700μm×H 100 gμm×L 20 mm) with poly (dimethylsiloxane) to observe adhesion of HL60 cells on type I collagen under continuous perfusion. The surface of poly (dimethylsiloxane) was treated by oxygen plasma and then coated by fetal calf serum proteins or chicken type I collagen. By using SDS-polyacrylamide gel electrophoresis, we showed that treatment of oxygen plasma had no effect on binding of serum proteins on the polymer surface. However, the amount of collagen on the surface increased by decreasing of the effect of plasma treatment. On the serum protein-coated polymer surface, HL60 cells fully flowed through under 0.2μL/min flow rate. On the other hand, HL60 cells bound to the collagen-coated polymer surface and subsequently adhered to the collagen-coated polymer surface. We suggest that the conformation of collagen would alter depending on the degree of oxygen plasma treatment of PDMS and HL60 cells would recognize the difference in collagen structure.本研究は近畿大学生物理工学部戦略的研究No.06-II-1, 2006およびNo.07-IV-3, 2007の助成を受けた,
  • シリコーン製マイクロウェルプレートと用いたウシ単一胚の個別培養  [Not invited]
    岩本 太作; 加藤 暢宏; 橋本 周; 河上 泰之; 谷口 俊仁; 庄 隼生; 岸 昌生; 細井 美彦; 松本 和也; 森本 義晴; 入谷 明; 佐伯 和弘
    The Journal of Reproduction and Development  2010/08
  • HASHIMOTO Shu; SAEKI Kazuhiro; KATO Nobuhiro; NAKAOKA Yoshiharu; MORIMOTO Yoshiharu
    Journal of mammalian ova research = 日本哺乳動物卵子学会誌  2010/04
  • ポリジメチルシロキサン製マイクロウェルの容積と胚数がウシ胚の初期発生に及ぼす影響  [Not invited]
    岩本 太作; 加藤 暢宏; 谷口 俊仁; 松井 孝徳; 高橋 千明; 中野 達也; 庄 隼生; 岸 昌生; 細井 美彦; 松本 和也; 入谷 明; 佐伯 和弘
    日本畜産学会大会講演要旨集  2010/03
  • 超音波加工によるマイクロウェルプレートの作成と応用
    加藤暢宏
    化学とマイクロ ナノシステム研究会講演要旨集, 2010  2010
  • ポリジメチルシロキサン製マイクロウェルを用いて作製したウシ体外受精胚の移植による受胎：地区学会長賞受賞講演．  [Not invited]
    谷口 俊仁; 佐伯 和弘; 加藤 暢宏; 岩本 太作
    平成21年度日本獣医師会学会年次大会  2010/01  宮崎市  平成21年度日本獣医師会学会年次大会、、
  • ポリジメチルシロキサン製マイクロウェルの容積がウシ胚の初期発生に及ぼす影響  [Not invited]
    岩本 太作; 加藤 暢宏; 谷口 俊仁; 松井 孝徳; 高橋 千明; 中野 達也; 庄 隼生; 岸 昌生; 細井 美彦; 松本 和也; 入谷 明; 佐伯 和弘
    日本胚移植学雑誌  2010/01
  • 減圧下で作製したPolydimethylsiloxane micro-wellを用いたヒト胚のtime-lapse photography．  [Not invited]
    佐伯 和弘; 加藤 暢宏; IVFなんばクリニック; IVFなんばクリニック
    第54回日本生殖医学会学術講演会  2009/11  金沢市  第54回日本生殖医学会学術講演会
  • 無孔性PDMS製マイクロウェルプレートによるヒト体外受精卵の培養  [Not invited]
    加藤 暢宏; 橋本 周; 森本 義晴; 佐伯 和弘
    日本バイオマテリアル学会大会予稿集  2009/11
  • HASHIMOTO MAKOTO; MORIMOTO YOSHIHARU; KATO NOBUHIRO; SAEKI KAZUHIRO
    日本生殖医学会雑誌  2009/10  (一社)日本生殖医学会
  • ポリジメチルシロキサン製マイクロウェルを用いて作製したウシ体外受精胚の移植による受胎．  [Not invited]
    佐伯 和弘; 加藤 暢宏; 岩本太作; 和歌山県畜産試験場; 和歌山県畜産試験場
    平成21年度近畿地区連合獣医師会獣医三学会（近畿）  2009/10  泉佐野市  平成21年度近畿地区連合獣医師会獣医三学会（近畿）
  • 減圧下で作製したPolydimethylsiloxane micro-wellを用いたヒト胚のtime-lapse photography  [Not invited]
    橋本 周; 森本 義晴; 加藤 暢宏; 佐伯 和弘
    日本生殖医学会雑誌  2009/10
  • Kato Nobuhiro; Taguchi Satoshi; Joe Yoshitaka; Suzuki Eisaku
    The JSME Symposium on Welfare Engineering  2009/09 
    A novel low-cost talking communication aid device utilizing touch panel for a disabled person with both dysphonia and athetosis type cerebral paralysis is developed.
  • PDMS製マイクロウェルプレートを用いたウシ胚の初期発生に及ぼすウェルの容積の影響  [Not invited]
    岩本 太作; 谷口 俊仁; 松井 孝徳; 高橋 千明; 中野 達也; 庄 隼生; 岸 昌生; 加藤 暢宏; 細井 美彦; 松本 和也; 入谷 明; 佐伯 和弘
    日本畜産学会大会講演要旨集  2009/09
  • 減圧下で作製したPolydimethylsiloxane well内で個別培養を行ったヒト胚の発育能．  [Not invited]
    佐伯 和弘; 加藤 暢宏; 岩本太作; IVFなんばクリニック; IVFなんばクリニック; IVFなんばクリニック; 和歌山県畜産試験場
    第50回日本哺乳動物卵子学会  2009/05  東京  第50回日本哺乳動物卵子学会
  • HASHIMOTO Shu; AKAMATSU Yoshie; IWAMOTO Daisaku; TANIGUCHI Toshihito; KATO Nobuhiro; SAEKI Kazuhiro; MORIMOTO Yoshiharu
    Journal of mammalian ova research = 日本哺乳動物卵子学会誌  2009/04
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ胚の体外培養の検討  [Not invited]
    岩本 太作; 松井 孝徳; 中野 達也; 高橋 千明; 谷口 俊仁; 加藤 暢宏; 佐伯 和弘
    日本胚移植学雑誌  2009/01
  • Development of bovine blastocysts following in vitro oocyte maturation on a dynamic microfluidic platform  [Not invited]
    C.L.Bormann; L.M.Cabrera; Y.S.Heo; N.Kato; S.Takayama; G.D.Smith
    American Society for Reproductive Medicine 64th Annual Meeting  2008/11
  • Poly-(Dimethylsiloxane)(PDMS) Micro Well Plates Cured Under Low Perssure for Bovine Embryo Culture  [Not invited]
    Nobuhiro Kato; Kazuhiro Saeki; Daisaku Iwamoto; Syunji Taniguchi
    Biomedical Engineering Society 2008 Annual Fall Meeting  2008/11  セントルイス  Biomedical Engineering Society
     
    低圧化で硬化させることで、ガス透過性を抑制したPDMSを用いてウシ受精卵の単一培養用のマイクロウェルプレートを試作した。人工授精を行ったウシ受精卵の培養実験において、集合培養と同等の発生率があることを確認した。
  • FUJII Masao; KATO Nobuhiro; NAKAGAWA Hideo; SHIMIZU Takaki; SATOMI Kenichi; TAGUCHI Shinya
    関西支部講演会講演論文集  2008/03
  • Rapid Prototyping of Microchannel for Braille Display Microfluidics using Printed Circuit Boards  [Not invited]
    Nobuhiro Kato; Yao-Kuang Chung; Shuichi Takayama
    Biomediacal Engineering Society 2007 Anuual Fall Meeting  2007/09  Los Angels, USA  Biomediacal Engineering Society 2007 Anuual Fall Meeting
     
    プリント基板を母型に用いることで、角部にRを持った台形形状断面を有するマイクロチャネルチップを迅速に安価に試作することができる事を示した。
  • Collaborative development of e-Learning solution in Pro/E operation training with industry and university  [Not invited]
    加藤 暢宏
    PTC user world event 2005 JAPAN  2005/11  PTC user world event 2005 JAPAN
  • 加藤 暢宏; 高野 功; 菊田 久雄
    センシングフォ-ラム資料  2005/09
  • Development of e-Learning contents in 3D-CAD operation training with factory-university collaboration  [Not invited]
    加藤 暢宏; 藤井 雅雄; 田口 晋也; 清水
    平成17年度 全国大学IT活用研究法法研究発表会 ２次選考発表会  2005/09  東京  平成17年度 全国大学IT活用研究法法研究発表会 ２次選考発表会
  • Improvement of polymer micro cantilevers for force controlled atomic force microscope  [Not invited]
    APMF2005 Proceedings  2005/08
  • 加藤 暢宏; 藤井 雅雄; 田口 晋也; 清水
    全国大学IT活用教育法法研究発表会 予稿集 （社） 私立大学情報教育協会  2005/07  （社） 私立大学情報教育協会
     
    産学協同による3次元CAD用e-Learning教材の教育効果について発表した。
  • KATO Nobuhiro; FUJII Masao; TAGUCHI Shinya; SHIMIZU Takaki
    関西支部講演会講演論文集  2005/03 
    This paper describes the development of an e-Learning solution in 3D CAD operation training. The e-Learning contents have following features. In operation, the contents indicate the instruction window and 3D CAD (Pro/ENGINEER[○!R]) window in same display simultaneously. The motif of the exercise models is familiar to class and enhances the motives for the exercise. The contents of the e-Learning are based on SCORM (Shareable Content Object Reference Model) standard and able to supervise the progress of the class individually. The effectiveness of the contents is confirmed by achievement check in design class exercises.
  • Development of e-Learning contents for 3D CAD operation  [Not invited]
    加藤 暢宏
    サイバネティック・フレキシブル・オートメーション（ＣＦＡ）研究分科会 第１９回研究例会  2005/03  大阪  サイバネティック・フレキシブル・オートメーション（ＣＦＡ）研究分科会 第１９回研究例会
     
    3次元CAD(ptc社製Pro/Engineer2001)を用いた機械設計教育を効果的に行うために，CAD操作スキルの習得用e-Learning教材を産学協同で開発した．開発の留意点・導入効果・３次元CADを活用したグループ設計について発表した．
  • Development of an e-Learning solution in 3D-CAD operation for design class  [Not invited]
    Proceedings of The 1st International Conference on Desing Engineering and Science  2005
  • 設計教育のための３次元CAD用e-Learning教材の開発  [Not invited]
    日本機械学会関西支部第８０期定時総会講演会講演論文集  2005
  • 微小力測定用ポリマーマイクロカンチレバー製作プロセスの改良  [Not invited]
    第２２回センシングフォーラム 資料  2005
  • N. Kato; C. S. Park; T. Matsumoto; H. Kikuta; K. Iwata
    Proceedings of the SICE Annual Conference  2004/12 
    In this paper, we report on the improvements in fabrication processes of polymer micro cantilevers for force controlled atomic force microscopy. The improvement of photo mask patterns results in the enhancement of controllability of the pyramidal probe tip geometry and in rapid dissolving of sacrificial layer. It also contributes to process simplicity.
  • SUZUKI Takayuki; OCHI Toshiaki; KATO Nobuhiro; WATANABE Toshiaki; MATSUMOTO Toshiro
    関西支部講演会講演論文集  2004/03 
    Performance of complex system included power generation by temperature difference and greenhouse heating using floating collector type solar pond with two-layer was investigated for simulation models. It was also compared with salt-gradient solar pond. In thermal collector part, thermal storage layer and soil, some equations of heat transfer were drawn to establish optimum conditions for design, respectively. They were given by analysis of complex heat transfer using simulation models. They were concluded as being the following: (1) Flow rate of the water circulation on the collectors was 50(kg/(m^2・h)). (2) Minimum incident solar radiation for starting of pumping was 500(W/m^2). (3) Depth of thermal storage layer is 3〜5(m). (4) Thickness of the insulation was more than 10(cm), (5) Surface area of pond is more than 100(m^2). Although the maximum water temperature in thermal storage layer was lower than salt-gradient solar pond, annual temperature hardly changed. Consequently, this system has advantage of use during winter was shown for this system. In addition, it was clearly presented that it is possible to use also for power generation and greenhouse heating.
  • Polymer micro cantilevers for force controlled atomic force microscopy 3 --- Improvement of Fabrication Process(共著)  [Not invited]
    Proceedings of SICE Annual Conference 2004 in Sapporo  2004
  • Nobuhiro Kato; Choong Sik Park; Toshiro Matsumoto; Hisao Kikuta; Koichi Iwata
    Proceedings of SPIE - The International Society for Optical Engineering  2003/12 
    We developed the fabrication process of all-plastic cantilever for force controlled atomic force microscope. The force controlled atomic force microscope can prevent damage during the scanning of the soft sample. However it has a drawback in its stability. To stabilize the system the cantilever is required to have small Q factor. The negative photo resist for MEMS (SU-8) has ideal properties, micromachinability and small Q factor, for this purpose. Here we demonstrate the cantilever consists of tip, lever and base with same polyimide. The mold of the probing tip is the pyramidal pit pattern on silicon wafer formed by direct leaser writing and anisotropic etching of silicon. Other elements of the cantilever are formed by contact lithography. The photo mask is also fabricated by direct laser writing. By arranging the dimensions of the cantilever, we can control its spring constant and resonant frequency. For the spring constant of 0.1N/m and the first resonant frequency of 2kHz, the typical dimensions of the cantilever is 1030 × 300 × 7 μm3. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • Polymer cantilevers for force controlled Atomic Force Microscope Desing and Fabrication  [Not invited]
    加藤 暢宏; 松本 俊郎; 朴忠植; 菊田久雄; 岩田
    APMF’2003/IMEKO TC3（中国）  2003/11  APMF’2003/IMEKO TC3（中国）
     
    力制御型原子間力顕微鏡を安定に動作させるための高分子材料製のカンチレバーの製作・評価について発表した。（英文）
  • Fabrication of polymer cantilevers for force-controlled atomic force microscope  [Not invited]
    加藤 暢宏; 松本 俊郎; 朴忠植; 菊田久雄; 岩田
    The International Symposium on OPTICAL SCIENCE AND TECHNOLOGY SPIE 48th Annual Meeting（アメリカ）  2003/08  The International Symposium on OPTICAL SCIENCE AND TECHNOLOGY SPIE 48th Annual Meeting（アメリカ）
     
    力制御型原子間力顕微鏡を安定に動作させるための高分子材料製のカンチレバーの設計・製作について発表した。（英文）
  • Kato Nobuhiro; Fujii Masao
    Proceedings of JSPE Semestrial Meeting  2003/03  2003年度精密学会春季大会（小金井）
     
    学部3年生を対象に3次元CAD（Pro/Engineer ptc社）を用いたグループワークを中心とした設計製図教育を行った。CAD操作の手戻りを極力さける為に、CADそのもののスキル教育よりもグループワークによる紙上での検討に重点を置いた。その結果、学生間の意志疎通の円滑化、コミュニケーション能力の向上が見られ、協調作業による3次元モデル構築の際にその効果が発揮された。本手法による教育効果について報告する。
  • Polymer cantilevers for force contreolled atomic force microscope - desing and fabrication -(共著)  [Not invited]
    Proceedings of APMF'2003  2003
  • Fabrication of polymer cantilevers for force controlled atomic force microscope(共著)  [Not invited]
    Proceedings of SPIE  2003
  • 加藤 暢宏; 萩野秀樹; 朴忠植; 横井昌幸; 岩田耕一; 菊田久雄; 三俣真理
    電気加工学会全国大会講演論文集  2002/11  電気加工学会全国大会（2002）（南埼玉）
     
    本研究では数kWクラスのCO2レーザ光の強度分布を所望の分布に整形するCGHの開発を目指している。今回は8レベルのCGHを高出力CO2レーザに適用した結果について述べた。
  • Education in product design in Kinki University in case of faculty of biology oriented science and technology  [Not invited]
    加藤 暢宏; 藤井 雅雄
    3次元CAD活用研究会9th Conference（川崎）  2002/11  3次元CAD活用研究会9th Conference（川崎）
     
    生物理工学部機械制御工学科では平成14年度から本格的に3次元CADによる設計教育を開始した。本講演では具体的な取り組み内容を紹介し、学生のコミュニケーション能力が明らかに向上したことを報告した。
  • FUJII Masao; KATO Nobuhiro; NISHIHAMA Megumi; SHIMIZU Takaki
    公開研究会・講演会技術と社会の関連を巡って : 技術史から経営戦略まで : 講演論文集  2002/10  日本機械学会（京都）
     
    The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. In the product design at multimedia age, it is important to promote the creative and intelligent design operation and also to construct the educational curriculum by using 3DCAD.
  • Microcantilever with High Damping Coefficent for Force Curve Messurement 2 Fabrication of Integrated Polyimide Cantilever  [Not invited]
    加藤 暢宏; 松本 俊郎; 朴忠植; 菊田久雄; 岩田
    2002年度精密工学会秋季大会学術講演会（熊本）  2002/10  2002年度精密工学会秋季大会学術講演会（熊本）
     
    本研究では、制御対象となるカンチレバーの減衰係数を大きくすることで制御系の安定性を向上させることを目的としている。レーザービーム描画装置による直接描画とマスク露光を使い分けることで、設計変更に柔軟に対応できる製作プロセスを確立できた。
  • 萩野 秀樹; 横井 昌幸; 朴 忠植; 加藤 暢宏; 三俣 真理; 菊田 久雄; 岩田 耕一
    精密工学会大会学術講演会講演論文集  2002/10
  • IWABU Akinori; MATSUMOTO Toshiro; KATO Nobuhiro; UOZATO Hiroshi
    年次大会講演論文集 : JSME annual meeting  2002/09  日本機械学会2002年度年次大会（東京）
     
    Glasses and contact lenses are popular solutions for many individuals with refractive disorders. Although many techniques have been proposed and tested by the laser eye surgery, photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK) are the most widely practiced by using excimer laser. A difficult problem for all refractive surgical procedures is to predict the final refraction because the intraocular pressure (IOP) applies inside the eyes. In this paper, mechanical analysis of cornea was carried out by using finite element method (FEM) and the optical performance of these models was calculated by ray tracng.
  • FUJII Masao; KATO Nobuhiro
    The proceedings of the JSME annual meeting  2002/09  日本機械学会、2002年度年次大会（東京）
     
    The rapid progress of semiconductor technology promotes the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer a CAD software and so on. This paper describes the progress of mechanical design technology and the importance of the education by using 3dimensional computer aided design (3DCAD) system at multimedia age.
  • Imaging polarimetry with an micro-retarder array  [Not invited]
    加藤 暢宏; 菊田久雄; 沼田紘治; 有光治人; 岩田耕一
    SICE Annual Conference 2002（大阪）  2002/08  SICE Annual Conference 2002（大阪）
     
    偏光画像計測のための×72画素からなるマイクロ移相子アレイを開発した。移相子アレイは異なる方向性を持った300nmピッチのポリイミド製のサブ波長回折格子を有している。製作には電子線描画と反応性イオンエッチングを用いた。
  • Polymer Micro Cantilevers for Force Controlled Atomic Force Microscopy 2 Fabrication of Cantilevers  [Not invited]
    加藤 暢宏; 松本 俊郎; 朴忠植; 菊田久雄; 岩田
    SICE Annual Conference 2002（大阪）  2002/08  SICE Annual Conference 2002（大阪）
     
    探針、カンチレバー、ベース部分が同一の樹脂によって形成されたAFM用のカンチレバーを開発した。形状をうまくコントロールすることでバネ定数と共振周波数を適正な値に制御した。ポリイミドの小さなQ値が系の安定性に寄与している。
  • Cooperation between an enterprise and a school in mechanical design education by using 3D・CAD  [Not invited]
    藤井 雅雄; 加藤 暢宏; 西濱恵 清水隆紀
    日本Pro/USER CONFERENCE 2002（東京）  2002/07  日本Pro/USER CONFERENCE 2002（東京）
     
    産学で連携して3次元CADを用いた設計教育を実施した。その目的は、企業内教育と学校内教育の整合性を検証し、学校内教育の充実をはかること、学生の就業意識を高めることである。
  • Development of micro cantilevers made of photosensitive polyimide  [Not invited]
    加藤 暢宏
    第 16 回高機能光学素子作製技術の確立 WG 会議 （大阪）  2002/01  第 16 回高機能光学素子作製技術の確立 WG 会議 （大阪）
     
    原子間力顕微鏡によるフォーカスカーブ測定の問題点について指摘し、 力制御型 AFM の優位性を説明し、 これを実現するための減衰率の高いカンチレバーの製作法について述べた。
  • IWABU Akinori; MATSUMOTO Toshiro; KATO Nobuhiro; UOZATO Hiroshi
    The proceedings of the JSME annual meeting  2002 
    Glasses and contact lenses are popular solutions for many individuals with refractive disorders. Although many techniques have been proposed and tested by the laser eye surgery, photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK) are the most widely practiced by using excimer laser. A difficult problem for all refractive surgical procedures is to predict the final refraction because the intraocular pressure (IOP) applies inside the eyes. In this paper, mechanical analysis of cornea was carried out by using finite element method (FEM) and the optical performance of these models was calculated by ray tracng.
  • Imaging polarimetery with an micro-retarder array(共著)  [Not invited]
    Proceedings of SICE Annual Conference 2002  2002
  • Polymer micro cantilevers for force controlled atomic force microscopy 2 - Fabrication of cantilevers -(共著)  [Not invited]
    Proceedings of SICE Annual Conference 2002 in Osaka  2002
  • マルチメディア時代における機械系設計教育について(共著)  [Not invited]
    日本機械学会年次大会講演論文集  2002
  • マルチレベルCGHによる炭酸ガスレーザビームシェイピング(共著)  [Not invited]
    精密工学会大会学術講演会講演論文集  2002
  • 高出力炭酸ガスレーザ用回折型ビーム整形素子の開発(共著)  [Not invited]
    電気加工学会全国大会講演論文集  2002
  • レーザによって切除された角膜のFEMによる応力的解析(共著)  [Not invited]
    日本機械学会年次大会講演論文集  2002
  • 力曲線測定のための高減衰マイクロカンチレバー 第２報 一体型ポリイミドカンチレバーの作成(共著)  [Not invited]
    2002年度 精密工学会秋季大会 講演論文集  2002
  • 産学で連携した3次元設計教育の取り組み(共著)  [Not invited]
    日本機械学会公開研究会･講演会講演論文集  2002
  • T. Matsumoto; A. Kojima; N. Kato; T. Watanabe; M. Tamiwa; M. Baba
    Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings  2001/12 
    The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1) the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • MIYAMURA Koji; MATSUMOTO Toshiro; KATO Nobuhiro; MIYAWAKI Shoichi; KAWAKAMI Tetsuji; BABA Masato; OKAJIMA Kaoru
    The Computational Mechanics Conference  2001/11
  • 加藤 暢宏; 三俣真理; 菊田久雄; 岩田耕一; 萩野秀樹; 朴忠殖; 四谷任
    電気加工学会全国大会講演論文集  2001/11  電気加工学会全国大会 （2001） （北九州）
     
    レーザ光収束用の銅の放物面鏡の表面曲面上へのホログラムの CGH パターン設計について検討した結果を述べた。
  • Stress Analysis of Temporomandibular Joint applied Masticatory Force by Finite Element Method  [Not invited]
    松本 俊郎; 宮村紘司; 加藤 暢宏; 岡嶋 馨; 宮脇正一; 川上哲司; 馬場
    第 14 回計算力学講演会 （札幌）  2001/11  第 14 回計算力学講演会 （札幌）
     
    下顎骨が咀嚼を行っている場合の顎関節の応力を関節円板の位置を考慮に入れて FEM により解析を行い考察した。
  • CO2 laser beam shaping by using hologram on a curved surface 2 Design and evaluation  [Not invited]
    加藤 暢宏; 三俣真理; 菊田久雄; 岩田耕一; 萩野秀樹; 朴忠殖; 四谷任
    2001 年度精密工学会秋季大会 （豊中）  2001/09  2001 年度精密工学会秋季大会 （豊中）
     
    レーザー収束用の放物面鏡上にパターンを描き、 収束とビーム整形の機能を有する曲面ホログラム素子の設計、 製作を行い、 整形されたビームの観測を行った。
  • 萩野 秀樹; 山口 勝己; 朴 忠植; 四谷 任; 豊田 宏; 加藤 暢宏; 三俣 真理; 菊田 久雄; 岩田 耕一
    精密工学会大会学術講演会講演論文集  2001/09
  • 萩野 秀樹; 山口 勝己; 朴 忠植; 四谷 任; 豊田 宏; 加藤 暢宏; 三俣 真理; 菊田 久雄; 岩田 耕一
    精密工学会大会学術講演会講演論文集  2001/09
  • IWABU Akinori; MATSUMOTO Toshiro; MATSUOKA Toshihiro; UOZATO Hiroshi; KATO Nobuhiro; UEDA Yukio
    生体・生理工学シンポジウム論文集  2001/08  第 16 回生体・生理工学シンポジウム （札幌）
     
    角膜の切除量と眼圧による角膜の変形を FEM により解析を行い考察した。
  • Polymer micro cantilevers for force controlled atomic force microscopy  [Not invited]
    加藤 暢宏; 松本 俊郎; 菊田久雄; 岩田
    第 40 回計測自動制御学会学術講演会 （名古屋）  2001/07  第 40 回計測自動制御学会学術講演会 （名古屋）
     
    本発表ではポリイミド製の高分子カンチレバーを提案した。 ポリイミドの機械的特性を実験的に得て、 力距離曲線の数値シミュレーションを行い、 原子間力分光法におけるフォースフィードバック測定のさらなる改良点について述べた。
  • Corneal Deformation Analysis with Finite Element Method in Laser Refractive Surgery  [Not invited]
    松本 俊郎; 加藤 暢宏; 魚里博
    Annual Meeting of ARVO (フロリダ)  2001/04  Annual Meeting of ARVO (フロリダ)
     
    光屈折矯正角膜切除術 PRK を受けた眼球の眼圧による眼球の変位および応力に関する力学解析をそれぞれ有限要素法 (FEM) により行い､ その結果について考察した｡
  • MATSUOKA Toshihiro; MATSUMOTO Toshiro; KATO Nobuhiro; UEDA Yukio; UOZATO Hiroshi
    バイオエンジニアリング講演会講演論文集  2001/01
  • Displacement analysis of cornea after laser ablation by  [Not invited]
    松本 俊郎; 松岡俊宏; 上田 幸雄; 加藤 暢宏; 魚里博
    第 13 回日本機械学会バイオエンジニアリング講演会 (仙台)  2001/01  第 13 回日本機械学会バイオエンジニアリング講演会 (仙台)
     
    角膜の混濁を除くために､ 光治療角膜切除術 (PTK) による治療が行われている｡ 切除により角膜の厚さが減少することから､ 眼圧により角膜が変形する｡ PTK による角膜の切除量と眼圧による眼球の力学解析を FEM により行った｡
  • レーザ表面改質のためのビームシェイピング 曲面上ホログラムの検討(共著)  [Not invited]
    電気加工学会全国大会講演論文集  2001
  • レーザによる角膜切除後のFEMによる眼球の変形解析(共著)  [Not invited]
    バイオエンジニアリング学術講演会講演論文集  2001
  • レーザによって角膜切除された眼球のFEMによる変形解析(共著)  [Not invited]
    生体･生理工学シンポジウム論文集  2001
  • そしゃく筋の作用を受ける顎関節の有限要素法による応力解析(共著)  [Not invited]
    日本機械学会計算力学講演会講演論文集  2001
  • 曲面上ホログラムを用いた炭酸ガスレーザビームシェイピング ２．設計と評価(共著)  [Not invited]
    2001年度 精密工学会秋季大会 講演論文集  2001
  • 曲面上ホログラムを用いた炭酸ガスレーザビームシェイピング １．ホログラム作成（共著）  [Not invited]
    2001年度精密工学会秋季大会 講演論文集  2001
  • 力制御型原子間力顕微鏡のためのポリマーマイクロカンチレバー(共著)  [Not invited]
    第40回計測自動制御学会学術講演会予稿集  2001
  • Holographic Investigation on the Deformation of the facial bone  [Not invited]
    松本 俊郎; 加藤 暢宏; 土田雅久; 植村和嘉; 杉村正仁; 挌内敏
    Tenth Int. Conf. on Biomedical Engineering (シンガポール)  2000/12  Tenth Int. Conf. on Biomedical Engineering (シンガポール)
     
    乾燥顔面骨について､ 顔面部を構成する頬骨､ 上顎骨等に荷重を加えた時､ 骨の変形をホログラフィ干渉法により測定した｡ その結果､ 境界面で骨縫合部を介して応力緩和が生じ骨の変形を抑制する効果があることがわかった｡
  • FUJIWARA Syosuke; MATSUMOTO Toshiro; KATO Nobuhiro; UEDA Yukio; KAWAKAMI Tetsuji; BABA Masato; MIYAWAKI Shoichi; OKAJIMA Kaoru
    The ... JSME Bioengineering Conference and Seminar  2000/10
  • 中村 泰浩; 八丁 久士; 菊田 久雄; 岩田 耕一; 加藤 暢宏
    2000年度精密工学会春季大会講演論文集  2000/03
  • FUJIWARA Syosuke; MATSUMOTO Toshiro; KATO Nobuhiro; UEDA Yukio; KAWAKAMI Tetsuji; BABA Masato; MIYAWAKI Shoichi; OKAJIMA Kaoru
    Proceedings of the JSME Bioengineering Conference and Seminar  2000
  • 松本 俊郎; 赤井 正哉; 本津 茂樹; 細井 美彦; 加藤 暢宏; 出水 敬; 上野谷 敏之
    学術講演会講演論文集  2000
  • Holographic investigation on the deformation of human femur due to the load(共著)  [Not invited]
    Proc. of the 12th Conf. of the European Soc. of Biomechanics  2000
  • POLYMER MICRO CANTILEVERS FOR FORCE CONTROL ATOMIC FORCE MICROSCOPY(共著)  [Not invited]
    Proceedings of the 5th Asia-Pacific Symposium on Measurement of Force, Mass and Torque  2000
  • ホログラフィー干渉法による荷重による中顔面骨の変形測定(共著)  [Not invited]
    顎顔面バイオメカニクス学会誌  2000
  • 生体親和性ハイドロキシアパタイト薄膜の付着強度(共著)  [Not invited]
    第44回日本学術会議材料研究連合講演会講演論文集  2000
  • 有限要素法による顎関節の力学的検討(共著)  [Not invited]
    日本機械学会第11回バイオエンジニアリング学術講演会講演論文集  2000
  • レーザーアブレーション法によるハイドロキシアパタイト薄膜の力学的評価(共著)  [Not invited]
    第49期日本材料学会学術講演会講演論文集  2000
  • エキシマレーザーアブレーションによるHAp薄膜の形成(共著)  [Not invited]
    顎顔面バイオメカニクス学会誌  2000
  • 力制御AFMのための高減衰ポリマー・カンチレバー(共著)  [Not invited]
    第61回応用物理学会学術講演会講演予稿集  2000
  • 走査型近視野原子間力顕微鏡によるウシ精子の表面形状の観察  [Not invited]
    佐伯 和弘; 加藤 暢宏; 細井 美彦; 松本 和也; 加藤 博己; 入谷 明
    日本胚移植学雑誌  1999/01
  • Deformation of cornea after ex cimer laser due to FEM(共著)  [Not invited]
    Invest. Opthalmol.  1999
  • 有限要素法によるエキシマレーザーPRK後の角膜変形と屈折率変化(共著)  [Not invited]
    第17回屈折率調節研究会  1999
  • レーザ角膜切除後のFEMによる応力解析(共著)  [Not invited]
    バイオエンジニアリング学術講演会･秋季セミナー講演論文集  1999
  • MATSUMOTO Toshiro; KATO Nobuhiro; TAGA Masao
    太陽/風力エネルギー講演論文集 = Proceedings of JSES/JWEA Joint Conference  1998/12
  • Matsumoto Toshiro; Nagae Sadahiko; Kato Nobuhiro
    Memoirs of the School of Biology-Oriented Science and Technology of Kinki University  1998/06 
    A semi-quantitative measurement of the strain distribution by two-dimensional color dispaly is presented. The strain components are converted into color band intensity distributions in the image of the specimen. The method is based on a optical technique with the aid of the spatial filtering theory. To show a usefulness of the method, it was carried out to measure the strain distribution of a plane plate with a round hole.
  • Relation between the loads applied to the facial bone and fracture patterns by means of holographic interferometry(共著)  [Not invited]
    Abstracts of the 3rd World Congress of Biomechanics  1998
  • Mechanical analysis of cornea by means of finite element method(共著)  [Not invited]
    Fifth International Conference on Optics Within Life Sciences (OWLS V)  1998
  • DYNAMIC MODEL ON FORCE FEEDBACK SYSTEM FOR NANO-NEWTON FORCE MEASUREMENT(共著)  [Not invited]
    PROCEEDINGS of IMEKOTC3/APMF'98  1998
  • 粘性安定化ソーラーポンドに用いるポリマー溶液の物理的特性(共著)  [Not invited]
    日本太陽エネルギー学会･日本風力エネルギー協会合同研究発表会講演論文集  1998
  • ホログラフィ干渉法による人大腿骨の変形測定(共著)  [Not invited]
    バイオエンジニアリング学術講演会講演論文集  1998
  • 走査型近視野原子間力顕微鏡によるウシ精子の表面観察(共著)  [Not invited]
    第５回日本胚移植研究会および第17回北海道牛受精卵移植研究会合同研究発表会  1998
  • フォース・カーブ測定における力フィードバック制御系の設計(共著)  [Not invited]
    第45回応用物理学会関係連合講演会講演予稿集  1998
  • 表面改質テフロン基板上へのハイドロキシアパタイト薄膜の製作(II)(共著)  [Not invited]
    第45回応用物理学会関係連合講演会講演予稿集  1998
  • レーザーアブレーションによって作成したHAp薄膜の生体材料への応用(共著)  [Not invited]
    日本機械学会第76期全国大会講演論文集  1998
  • 人大腿骨の3次元有限要素法による応力解析(共著)  [Not invited]
    日本機械学会第76期全国大会講演論文集  1998
  • 力フィードバック方式による力距離曲線測定のための安定化制御(共著)  [Not invited]
    第44回応用物理学会関係連合講演会講演予稿集  1997
  • 表面改質テフロン基板上へのハイドロキシアパタイト薄膜の製作(共著)  [Not invited]
    第58回応用物理学会学術講演会講演予稿集  1997
  • Force feedback sensors for AFM (共著)  [Not invited]
    Proceedings of Measurements of Force and Mass  1996
  • 光ファイバ干渉計を用いた力バランス方式による微小力検出(共著)  [Not invited]
    第43回応用物理学会関係連合講演会講演予稿集  1996
  • 力バランス方式による微小力測定器(共著)  [Not invited]
    第35回計測自動制御学会学術講演会予稿集  1996
  • 鈴木 一平; 加藤 暢宏; 菊田 久雄; 岩田 耕一
    精密工学会大会学術講演会講演論文集  1995/09
  • 力バランス型AFMによるForce Curveの面分布測定(共著)  [Not invited]
    1995年精密工学会秋季大会講演論文集  1995
  • Surface force detection using a force balancing method with an optical displacement sersor (共著)  [Not invited]
    Proceedings of the XIII IMEKO world congress  1994
  • 力バランス型AFMによる力-距離曲線の測定(共著)  [Not invited]
    第55回応用物理学会学術講演会講演予稿集  1994
  • 光てこを用いた力バランス法による表面力の検出(共著)  [Not invited]
    第54回応用物理学会学術講演会講演予稿集  1993

Affiliated academic society

  • JAPANESE SOCIETY FOR MEDICAL AND BIOLOGICAL ENGINEERING   THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS   精密工学会   日本機械学会   応用物理学会   

Works

  • 2次元レーザービーム描画装置の改良(3次元曲面描画への適用)
    2001

Research Themes

  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2023/04 -2028/03 
    Author : 大橋 拓矢; 加藤 暢宏; 平井 慶充; 矢田 由美; 房本 安矢; 井口 豪人
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2024/04 -2027/03 
    Author : 加藤 暢宏; 古薗 勉; 田中 篤
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    Date (from‐to) : 2022/04 -2027/03 
    Author : 西村 好晴; 田中 篤; 加藤 暢宏
     
    【はじめに】Stanford A型急性大動脈解離（ATAAD）の手術に際して解離腔の補強は重要である。現在用いているバイオグルーは有効であるが組織毒性の問題があり、フィブリン糊は即時的接着力が弱い。我々は両方向性にマイクロニードルを有する非生物由来の生分解性材料シートを人工中膜として解離腔に挿入し、解離腔の補強に応用できないかと考えている。【方法】ポリ乳酸を素材としメッシュ状のシートを3Dプリンターで作成する。マイクロニードルの雄型、雌型を形成し、メッシュ状シートを合わせて熱プレスにより表裏両方向性マイクロニードルシートを作成する。ブタ胸部大動脈組織片を用いて大動脈壁を用手的に剥離し、内膜側と外膜側の間に2cm大の人工中膜（ニードルの高さ：上方向950㎛、下方向500㎛、シートの厚み：260㎛）を挟み、フィブリン糊単独（F群）と人工中膜＋フィブリン糊（MN群）の2群で牽引力を比較した。牽引力の測定にはデジタルフォースゲージを用いた。長軸方向と半径方向でそれぞれ牽引実験を行った。【結果】長軸方向の牽引力（n=5）はF群1.5±0.6N、MN群3.7±0.7Nで、MN群で有意に高値であった。半径方向の牽引力（n=6）はF群0.9±0.5N、MN群1.5±0.3Nで、MN群で有意に高値であった。さらに、ブタを用いて体外循環下に下行大動脈を遮断し、横切開を行い、部分的に大動脈解離を作成した。そこに人工中膜を挿入し、縫合を行う実験を行った。針の運針には問題を認めなかった。【まとめ】我々が新たに開発した人工中膜は解離腔の接着力を増強することが示唆された。人工中膜は急性期の接着力増強以外に、慢性期においてのマイクロニードルの生体吸収によるコラーゲン架橋によりより強固な解離空の閉鎖が期待されるのではないかと考えており、今後、人工中膜の至適形状の検討と慢性期の組織学的評価が必要と考えている。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    Date (from‐to) : 2020/04 -2025/03 
    Author : 本田 賢太朗; 田中 篤; 加藤 暢宏; 西村 好晴; 湯崎 充
     
    本研究は、心臓血管外科手術における止血をテーマとし、新たな止血デバイスを開発するという研究である、従来のような生物由来製品を使用しない生体吸収素材を用いて、より安価で感染riskのない止血シートを開発することを目的としている。 止血シートの条件としては、可動性のある心臓および血管組織に安定して、止血までの一定時間固定可能であることが必要となる。 本研究では止血シートにマイクロニードルを使用することで、心臓大血管という動きのある組織においても十分な接着効果があるのか評価を行う。 今年度は止血シートの柔軟性を改良するという点で、シートの網目構造を何種類か作成し、よりfittingの良いシートを作成した。また組織への強固な接着のためにニードルの長さも350μm、700μmと作成した。動物実験を予定したがCOVIDのため実験が中止となっている。2022.6月に実験を行う予定としている。 6月予定の実験としてはブタの大動脈縫合部位上でのシートのfitting、右室心筋あるいは右房心筋上での組織との接着性について評価を行う。それぞれ心筋に同程度の傷を作成し、ニードルの高さによって接着性が異なるのか、他の既存の止血用の製品との比較も行い本製品の有用性についての検討を行う予定としている。またフォースゲージを用いた組織との接着実験も予定しており、どの程度のずり応力に耐えることが可能か測定予定としている。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2020/04 -2023/03 
    Author : Kato Nobuhiro
     
    Blood products used for hemostasis have a small but significant risk of infection. A novel device that can achieve hemostasis in a short time using non-biologically derived materials is needed. In this research project, we focused on microneedles (MN), which have been developed as a percutaneous drug delivery device. By applying an MN patch consisting of an array of microneedles to the surface of an organ that requires hemostasis during a surgical procedure, it is possible to promote hemostasis by physically immobilizing and holding the wound surface. Aiming to apply clinical application, we have established a method of increasing the size of the MN le patch and clarified the mechanical properties of the flexible mesh-like MN patch that does not inhibit organ movement.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2014/04 -2017/03 
    Author : SAEKI Kazuhiro; HASHIMOTO Shu
     
    We produced PDMS micro-wells connected with narrow canals. Bovine oocytes were introduced into the wells and medium was changed through the canals, but oocytes were trapped into the canals. Therefore, we developed simple bovine IVP systems. Bovine oocytes were introduced into test tubes filled with maturation medium. The oocytes were matured then only medium was changed by small pipets with IVF medium. The oocytes were fertilized, then medium was then changed with development medium. The IVF oocytes were cultured for 168 hours.The blastocyst rates were the same with the control method. We also examine the cleavage timing of bovine IVF oocytes with small TLC device which we originally produced. We can predict blastocyst development by examining the cleavage timing.
  • 科学技術振興機構：産学が連携した研究開発成果の展開 研究成果展開事業 マッチングプランナープログラム
    Date (from‐to) : 2016 -2016 
    Author : 加藤 暢宏
  • 産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 探索タイプ
    Date (from‐to) : 2013 -2013 
    Author : 加藤 暢宏
     
    生殖補助医療の高度化とコスト低減に寄与する、培養細胞の常時監視を実現する安価なデジタル倒立顕微鏡システムの開発を行った。3次元CADを高度に活用する事により、インキュベータ内に設置可能で、胚の卵割を常時監視できる性能を持った装置を開発する事ができた。無限遠補正系の顕微光学系と高解像度の撮像素子を採用する事により、高い画質を持った装置となった。対物レンズを駆動するための機構・電子回路を設計・製作する事で、制御用PCからの遠隔操作でピント調整、撮影を行う事ができた。一方で光学系の変更に伴い、装置の奥行きは若干長くなってしまったが、当初の目的であるインキュベータに内蔵する事には支障はない。実際の培養細胞の観察による評価には至らなかったが、今後早急に評価を行う。
  • 日本学術振興会：科学研究費助成事業 奨励研究(A)
    Date (from‐to) : 1999 -2000 
    Author : 加藤 暢宏
     
    原子間力分光の実用化を目指して、Force Curve測定の高精度化を行うために、以下の二つのアプローチで研究を行った。 1.力平衡を実現するためのフォース・フィードバック系の安定制御を目指した理論的解析 フォースフィードバック系の安定には、カンチレバーのQ値およびバネ定数、制御器の遮断周波数とゲインが関与していることを明らかにした。中でも、Q値は材質によって決定されるため重要である。現在この結果を基に、系を安定化させるために必要な低いQ値を持った微細加工可能な高分子材料を用いてカンチレバーの最適形状設計を行っている。詳細は第61回応用物理学会学術講演会およびAPMF2001にて発表した。 2.コンタミネーション除去をねらった真空チェンバー内でのForce Curve測定 実際のForce Curve測定を真空内で行うためには、探針と試料の近接を遠隔操作で行う必要がある。そのための方法として3軸ピエゾ微動ステージでの振動駆動による試料の輸送について検討した。ステージに移動方向とある角度をなす正弦波を印加する事で試料の輸送を行うことに成功したが、満足な再現性を得ることができず、発表には至っていない。 今後の課題としては多数のフィードスルーを設けたことによるチェンバーの気密性の悪化を改善する必要がある。また、制御系のパラメータをチューニングする際にアナログ系では自由度に限界があるため高速なデジタル制御を導入する必要がある。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 1998 -2000 
    Author : MASTUMOTO Toshiro; KATO Nobuhiro; KIKUTA Hisao
     
    Feasibility of polyimide cantilever for force feedback method is studied. The stability of the force feedback system is mainly improved by small Q factor and the low resonant frequency of the cantilever. The polyimide cantilever is fabricated and investigated its mechanical feature, especially Q factor in a vacuum. Form these values the required bandwidth of the force feedback system is estimated. The polyimide cantilever reduces the frequency requirement to 1/20, compared with the SiN cantilever with same feature except for internal damping. The system stability is confirmed by numerical simulations. The bandwidth shortage results in large oscillation of the force curve. To increase biological compatibility of Ti-6Al-4V, thin film of hydroxyapatite (HAp) was deposited on the polished substrate(0.1〜3.0μmR_) by ArF excimer laser in an atmosphere of O_2+H_2O.The gas pressure was kept at 13.3Pa (1×10^<-1>Torr) during the deposition. The substrate temperature was varied torn 500℃ to 560℃. Crystal stractures of HAp film were evaluated by X-ray diffraction (XRD). At temperatures less than 500℃ the films resulted in amorphous. At temperatures greater than 560℃ the films had mixture phase of crystallized HAp and tricalcium phosphate (TCP). Surface morphology of HAp film was observed by atomic-force microscope (AFM). Mechanical properties of film were measured by the scratch test and the peeling off test by using tensile test machine. The following results were obtained : (1) The crystallized HAp was obtained at about 530℃, (2) In the scratch test, HAp film was clearly removed at the load of 18N, (3) In the peeling off test, the adhesive strength of HAp film on the substratewere about 2.3, 5.4 and 10MPa for the surface roughness 0.1, 1.0 and 3.0μmRmax, respectively and (4) Biocompatibility of HAp film was evaluated by in-vitro. Unfortunately, to investigate biological specimen the stability of the force control atomic force microscopy system is not sufficient. Farther improvement of the force control system is required.

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