NAKASONE Kaoru

Researcher Information

J-Global ID

• 200901044332179700

Research Interests

• ゲノム解析   植物病原菌   黒麹菌   高度好塩性アーキア   深海微生物   極限環境微生物   Genomics   Extremely halophilic archaeon   Deep-sea microorganism   

Research Areas

• Life sciences / Applied biochemistry
• Life sciences / Functional biochemistry
• Life sciences / Genetics

Academic & Professional Experience

• 1994/04 - 2001/03  The DEEP STAR Group, Japan Marine Science and Technology CenterResearch Associate
• 1987/04 - 1988/03  東京大学 海洋研究所海洋生化学部門研究生

Education

• 1990/04 - 1994/03  University of the Ryukyus  Graduate School of Medicine  形態機能系
• 1988/04 - 1990/03  University of the Ryukyus  大学院理学研究科修士課程  生物学専攻
• 1983/04 - 1987/03  University of the Ryukyus  Faculty of Science  Department of Marine Science

Association Memberships

• Japan Association of Food Preservation Science (JAFPS)   The Japanese Biological Safety Association (JBSA)   Japanese Society for Living Systems Design Research   American Society for Microbiology   The Japanese Society for Extremophiles   Japan Society for Bioscience, Biotechnology, and Agrochemistry   日本分子生物学会   

Published Papers

• Draft Genome Sequence of Saccharomyces cerevisiae DJJ01, Isolated from Dojoji Temple in Gobo, Wakayama, Japan

  Shinnosuke Okuhama; Kaoru Nakasone; Kazuki Yamanaka; Chiho Miyazaki; Tsumugi Nakamoto; Yuki Nakashima; Masataka Kusube

  Microbiology Resource Announcements 11 (8) 2022/08 [Refereed]
  
• Sterilization Effects of HO2/O2-Radicals Produced by H2O-O2 Plasma

  Kosei Satahira; Kaoru Nakasone; Tatsuhiko Ihara

  Journal of Photopolymer Science and Technology 29 (3) 433 - 438 2016/06 [Refereed]
  
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• Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica

  Rie Yatsunami; Ai Ando; Ying Yang; Shinichi Takaichi; Masahiro Kohno; Yuriko Matsumura; Hiroshi Ikeda; Toshiaki Fukui; Kaoru Nakasone; Nobuyuki Fujita; Mitsuo Sekine; Tomonori Takashina; Satoshi Nakamura

  FRONTIERS IN MICROBIOLOGY FRONTIERS RESEARCH FOUNDATION 5 (5) 100 - 104 1664-302X 2014/03 [Refereed]
   

  The carotenoids produced by extremely halophilic archaeon Haloarcula japonica were extracted and identified by their chemical, chromatographic, and spectroscopic characteristics (UV-Vis and mass spectrometry). The composition (mol%) was 68.1% bacterioruberin, 22.5% monoanhydrobacterioruberin, 9.3% bisanhydrobacterioruberin, <0.1% isopentenyldehydrorhodopin, and trace amounts of lycopene and phytoene. The in vitro scavenging capacity of a carotenoid, bacterioruberin, extracted from Haloarcula japonica cells against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was evaluated. The antioxidant capacity of bacterioruberin was much higher than that of beta-carotene.
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• Transglycosylation activity of intracellular starch-active enzyme MalA from extremely halophilic archaeon Haloarcula japonica

  Onodera Masahiko; Yatsunami Rie; Fukui Toshiaki; Nakasone Kaoru; Fujita Nobuyuki; Sekine Mitsuo; Takashina Tomonori; Nakamura Satoshi

  Journal of Applied Glycoscience Supplement The Japanese Society of Applied Glycoscience 2011 56 - 56 2011
• Cloning and characterization of dihydrofolate reductases from deep-sea bacteria

  Chiho Murakami; Eiji Ohmae; Shin-ichi Tate; Kunihiko Gekko; Kaoru Nakasone; Chiaki Kato

  JOURNAL OF BIOCHEMISTRY OXFORD UNIV PRESS 147 (4) 591 - 599 0021-924X 2010/04 [Refereed]
   

  Enzymes from organisms living in deep-sea are thought to have characteristic pressure-adaptation mechanisms in structure and function. To better understand these mechanisms in dihydrofolate reductase (DHFR), an essential enzyme in living cells, we cloned, overexpressed and purified four new DHFRs from the deep-sea bacteria Shewanella violacea (svDHFR), Photobacterium profundum (ppDHFR), Moritella yayanosii (myDHFR) and Moritella japonica (mjDHFR), and compared their structure and function with those of Escherichia coli DHFR (ecDHFR). These deep-sea DHFRs showed 33-56% primary structure identity to ecDHFR while far-ultraviolet circular dichroism and fluorescence spectra suggested that their secondary and tertiary structures were not largely different. The optimal temperature and pH for deep-sea DHFRs activity were lower than those of ecDHFR and different from each other. Deep-sea DHFRs kinetic parameters K-m and k(cat) were larger than those of ecDHFR, resulting in 1.5-2.8-fold increase of k(cat)/K-m except for mjDHFR which had a 28-fold decrease. The enzyme activity of ppDHFR and mjDHFR (moderate piezophilic bacteria) as well as ecDHFR decreased as pressure increased, while svDHFR and myDHFR (piezophilic bacteria) showed a significant tolerance to pressure. These results suggest that DHFRs from deep-sea bacteria possess specific enzymatic properties adapted to their life under high pressure.
• Expression and characterization of a novel glucoamylase from extremely halophilic archaeon Haloarcula japonica

  Kiyohara Mie; Onodera Masahiko; Yatsunami Rie; Fukui Toshiaki; Nakasone Kaoru; Fujita Nobuyuki; Sekine Mitsuo; Takashina Tomonori; Nakamura Satoshi

  Journal of Applied Glycoscience Supplement The Japanese Society of Applied Glycoscience 2010 38 - 38 2010
• Genetic analysis and characterization of an intracellular α-amylase from extremely halophilic archaeon Haloarcula japonica

  Onodera Masahiko; Yatsunami Rie; Fukui Toshiaki; Nakasone Kaoru; Fujita Nobuyuki; Sekine Mitsuo; Takashina Tomonori; Nakamura Satoshi

  Journal of Applied Glycoscience Supplement The Japanese Society of Applied Glycoscience 2010 37 - 37 2010
• Molecular cloning and functional characterization of α-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith)

  Fengnian Yu; Sho Okamto; Kaoru Nakasone; Kyoko Adachi; Satoru Matsuda; Hisashi Harada; Norihiko Misawa; Ryutaro Utsumi

  Planta 227 (6) 1291 - 1299 0032-0935 2008/05 

  Shampoo ginger (Zingiber zerumbet Smith) has a high content and large variety of terpenoids in the essential oil of its rhizome. Here, we report on the isolation of a cDNA clone (ZSS1) encoding α-humulene synthase, a possible key enzyme of zerumbone biosynthesis. This clone contains an open reading frame of 1,644 bp and is predicted to encode a protein of 548 amino acids with a calculated molecular mass of 64.5 kDa. The deduced amino acid sequence shows 34-63% identity with known sesquiterpene synthases of other angiosperm species. Based on exon-intron organization, ZSS1 is classified as the terpene synthase-III (TPS-III) subfamily. When expressed in Escherichia coli, the recombinant enzyme catalyzed the formation of a major product, α-humulene (95%) and a minor by-product, β-caryophyllene (5%). Introduction of ZSS1 and the foreign mevalonate pathway involved in FPP synthesis into E. coli results in in vivo production of α-humulene. Transcript of ZSS1 was detected almost exclusively in rhizomes and was up-regulated in both leaves and rhizomes after treatment with methyl jasmonate (MeJA), suggesting its ecological function in shampoo ginger. © 2008 Springer-Verlag.
• Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith)

  Fengnian Yu; Sho Okamto; Kaoru Nakasone; Kyoko Adachi; Satoru Matsuda; Hisashi Harada; Norihiko Misawa; Ryutaro Utsumi

  PLANTA SPRINGER 227 (6) 1291 - 1299 0032-0935 2008/05 

  Shampoo ginger (Zingiber zerumbet Smith) has a high content and large variety of terpenoids in the essential oil of its rhizome. Here, we report on the isolation of a cDNA clone (ZSS1) encoding alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis. This clone contains an open reading frame of 1,644 bp and is predicted to encode a protein of 548 amino acids with a calculated molecular mass of 64.5 kDa. The deduced amino acid sequence shows 34-63% identity with known sesquiterpene synthases of other angiosperm species. Based on exon-intron organization, ZSS1 is classified as the terpene synthase-III (TPS-III) subfamily. When expressed in Escherichia coli, the recombinant enzyme catalyzed the formation of a major product, alpha-humulene (95%) and a minor by-product, beta-caryophyllene (5%). Introduction of ZSS1 and the foreign mevalonate pathway involved in FPP synthesis into E. coli results in in vivo production of alpha-humulene. Transcript of ZSS1 was detected almost exclusively in rhizomes and was up-regulated in both leaves and rhizomes after treatment with methyl jasmonate (MeJA), suggesting its ecological function in shampoo ginger.
• The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12

  Tamegai, H; Chikuma, S; Ishii, M; Nakasone, K; Kato, C

  DNA Sequence 19 308-312  2008 [Refereed]
  
• Roles of a short connecting disulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c(5)*

  Ogawa Keiko; Sonoyama Takafumi; Takeda Taku; Ichiki Shin-ichi; Nakamura Shota; Kobayashi Yuji; Uchiyama Susumu; Nakasone Kaoru; Takayama Shin-ichi J; Mita Hajime; Yamamoto Yasuhiko; Sambongi Yoshihiro

  EXTREMOPHILES 11 (6) 797 - 807 1431-0651 2007/11 [Refereed]
   

  Cys-59 and Cys-62, forming a disulfide bond in the four-residue loop of Shewanella violacea cytochrome c (SV cytc ), contribute to protein stability but not to redox function. These Cys residues were substituted with Ala in SV cytc , and the structural and functional properties of the resulting C59A/C62A variant were determined and compared with those of the wild-type. The variant had similar features to those of the wild-type in absorption, circular dichroic, and paramagnetic H NMR spectra. In addition, the redox potentials of the wild-type and variant were essentially the same, indicating that removal of the disulfide bond from SV cytc does not affect the redox function generated in the vicinity of heme. However, calorimetric analysis of the wild-type and variant showed that the mutations caused a drastic decrease in the protein stability through enthalpy, but not entropy. Four residues are encompassed by the SV cytc disulfide bond, which is the shortest one that has been proved to affect protein stability. The protein stability of SV cytc can be controlled without changing the redox function, providing a new strategy for regulating the stability and function of cytochrome c. © 2007 Springer. 5 5 5 5 5 5 1
• Discoveries of deep-Sea piezophiles, and their pressure adapted enzymes

  Kato Chiaki; Sato Takako; Abe Fumiyoshi; Ohmae Eiji; Tamegai Hideyuki; Nakasone Kaoru

  Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology Japanese Research Group of High Pressure Bioscience and Biotechnology 1 (1) 47-52 - 121 2007 

  The psychrophilic, moderately piezophilic bacterium Shewanella violacea strain DSS12 is a deep-sea isolate from a sediment sample collected at the Ryukyu Trench (depth: 5,110 m), which grows optimally at 30 MPa and 8°C but also grows at atmospheric pressure (0.1 MPa) and 8°C. We have examined this strain to elucidate the molecular basis for gene and protein regulations at different pressure conditions because this strain is useful as a model bacterium for comparing the various features of bacterial physiology under pressure conditions. Proteins, from such deep-sea adapted piezophiles, could be active under high-pressure conditions in general. Actually atmospheric pressure adapted proteins can be inactive under higher-pressure conditions. For bio-processing under pressure, people are looking for pressure-tolerant enzymes, thus, “piezophilic proteins” would be focus on such industrial applications. In the case of respiratory proteins, cell divisional protein FtsZ, RNA polymerase subunit structure, and dihydrofolate reductase (DHFR), piezophilic proteins were unique for adaptation to high-pressure environment and some of them were more stable and active under higher pressure conditions.
• Protein-DNA interactions under high-pressure conditions, studied by capillary narrow-tube electrophoresis.

  Kawano H; Nakasone K; Abe F; Kato C; Yoshida Y; Usami R; Horikoshi K

  Bioscience, biotechnology, and biochemistry 7 69 (7) 1415 - 1417 0916-8451 2005/07 [Refereed]
   

  The method of electrophoretic mobility shift assay under high-pressure conditions was improved using a high-pressure electrophoresis apparatus with capillary narrow-tube gel. It was found that the protein–DNA complex in the gel was stained as a high-resolution spot with ethidium bromide. Using this method, it was found that the behavior under high-pressure conditions of the protein–DNA complex composed of NtrC protein and its target promoter DNA is important for the pressure-regulated transcription process, and it was confirmed that the complex was dissociated above a pressure of 70 MPa.
• Pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium Shewanella violacea DSS12.

  Tamegai H; Kawano H; Ishii A; Chikuma S; Nakasone K; Kato C

  Extremophiles : life under extreme conditions 3 9 (3) 247 - 253 1431-0651 2005/06 [Refereed]
  
• Identification of rpoBC genes encoding for beta and beta' subunits of RNA polymerase in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12.

  Kawano H; Nakasone K; Abe F; Kato C; Yoshida Y; Usami R; Horikoshi K

  Bioscience, biotechnology, and biochemistry 3 69 (3) 575 - 582 0916-8451 2005/03 [Refereed]
   

  RNA polymerase from cells of the deep-sea bacterium Shewanella violacea DSS12 was purified using three chromatographic steps. An in vitro transcription assay indicated that the purified enzyme was σ⁷⁰ containing RNA polymerase. The enzyme activity was inhibited in the presence of rifampicin when the sensitive domain was targeted. The rpoBC genes encoding for the β and β′ subunits of RNA polymerase were cloned and their nucleotide sequences determined. Expression plasmids, designated pQSVB and pQSVC, to overproduce these proteins were constructed, and the proteins were purified using a Ni²⁺ affinity column. In vitro reconstitution using all proteins for the holoenzyme (α, β, β′, σ⁷⁰) was carried out and the activity of the recombinant RNA polymerase was detected.
• Analysis of hydrostatic pressure effects on transcription in Escherichia coli by DNA microarray procedure.

  Ishii A; Oshima T; Sato T; Nakasone K; Mori H; Kato C

  Extremophiles : life under extreme conditions 9 (1) 65 - 73 1431-0651 2005/02 [Refereed]
  
• Cloning and characterization of the rpoE gene encoding an RNA polymerase σE factor from the deep-sea piezophilic Shewanella violacea strain DSS12

  Hiroaki KAWANO; Fumiyoshi ABE; Kaoru NAKASONE; Chiaki KATO; Yasuhiko YOSHIDA

  DNA Sequence 16 (1) 69 - 74 2005/01 [Refereed]
  
• Pressure-dependent activity of dihydrofolate reductase from a deep-sea bacterium Shewanella violacea strain DSS12

  Eiji Ohmae; Kazumasa Kubota; Kaoru Nakasone; Chiaki Kato; Kunihiko Gekko

  Chemistry Letters 33 (7) 798 - 799 0366-7022 2004/07 [Refereed]
   

  A new dihydrofolate reductase (svDHFR) was purified from a deep-sea bacterium, Shewanella violacea strain DSS12, isolated from the Ryukyu Trench (5110m). In contrast with E. coli DHFR, the enzyme activity of svDHFR increased with increasing hydrostatic pressure up to 100 MPa, suggesting that the enzyme kinetics and structural fluctuation of svDHFR are adapted to a high-pressure environment.
• Cloning and Overproduction of the rpoZ Gene Encoding an RNA Polymerase ω Subunit from a Deep-sea Piezophilic Shewanella violacea Strain DSS12

  Hiroaki Kawano; Yasuo Suzaki; Junko Fukuchi; Kaoru Nakasone; Fumiyoshi Abe; Chiaki Kato; Yasuhiko Yoshida; Ron Usami; Koki Horikoshi

  DNA Sequence 15 (2) 118 - 122 2004/07 [Refereed]
  
• Whole-genome analysis of deep-sea piezophilic and psychrophilic bacterium, Shewanella violacea strain DSS12

  NAKASONE Kaoru

  Nippon Nōgeikagaku Kaishi Japan Society for Bioscience, Biotechnology, and Agrochemistry 78 (4) 402 - 406 0002-1407 2004/04
• Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12.

  Kawano H; Ikegami A; Nakasone K; Kato C; Usami R; Horikoshi K

  Bioscience, biotechnology, and biochemistry 9 67 (9) 1983 - 1985 0916-8451 2003/09 [Refereed]
   

  NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.
• Molecular mechanisms of pressure-regulation at transcription level in piezophilic bacteria

  C Kato; K Nakasone; A Ikegami; H Kawano; R Usami; K Horikoshi

  TRENDS IN HIGH PRESSURE BIOSCIENCE AND BIOTECHNOLOGY, PROCEEDINGS ELSEVIER SCIENCE BV 19 211 - 218 0921-0423 2002 [Refereed]
   

  Deep-sea bacteria have unique systems for gene and protein expression controlled by hydrostatic pressure. One of the sigma factors, sigma(54), was found to play an important role on the pressure-regulated transcription in a deep-sea piezophilic bacterium, Shewanella violacea. A glutamine synthetase gene (glnA) has been targeted as a model for the pressure-regulated promoter to investigate the transcriptional regulation by the sigma(54) factor. Recognition sites for sigma(54) and sigma(70) factors were observed at an upstream region of the glnA and also NtrC-binding sites were identified at this same region. Primer extension analyses revealed that the transcription initiation sites of both promoters were determined and that the transcription from the sigma(54) site was regulated by elevated pressure. The sigma(54) promoter is known to be activated by a two components signal transduction system, NtrB-NtrC phospholylated relay. Our results suggested that this system might be regulated by deep-sea conditions and that the gene expression controlled by the sigma(54) promoter was actually regulated by pressure. We proposed a possible model of the molecular mechanisms for pressure-regulated transcription.
• Characterization and comparative study of the rrn operons of alkaliphilic Bacillus halodurans C-125

  K Nakasone; N Masui; Y Takaki; R Sasaki; G Maeno; T Sakiyama; C Hirama; F Fuji; H Takami

  EXTREMOPHILES SPRINGER JAPAN KK 4 (4) 209 - 214 1431-0651 2000/08 

  The ribosomal RNA operons (rrn) of alkaliphilic Bacillus halodurans C-125 were characterized and compared with those of B. subtilis. We isolated clones containing rrn operons from a lambda phage library of the C-125 chromosome, and the complete nucleotide sequence of each was determined. Eight rrn operons were identified by PFGE analysis of the C-125 chromosome digested with I-CeuI. The transcriptional orientation of the rrn operons mapped on the chromosome by Southern hybridization analysis was the same as the direction of replication of the chromosome. These operons were designated as rrnA-H, starting from the oriC locus in clockwise rotation. Sequence and structural analyses of these operons suggested that six of the rrn operons in the C-125 chromosome, rrnA, rrnB, rrnC-rrnD, rrnE, and rrnH, correspond to rrnO, rrnA, rrnJ-rrnW, rrnI, and rrnD in B. subtilis, whereas the other rrn operons (rrnF and rrnG) were specifically observed in C-125. The rrn loci were positioned from 0 degrees to 90 degrees on the physical map, with the oriC locus assigned the position zero degrees. Two ORFs annotated as tnpA and ykfC, whose gene products are likely to act as transposases, were found downstream of these six operons. Comparative analysis of the 16S-23S and 23S-5S ITS (internally transcribed sequence) regions of B. halodurans C-125 and those of B. subtilis revealed that the ITS regions in C-125 were much longer than those in B. subtilis. There was no substantial difference in the length of potential promoter sequences in B. halodurans and B. subtilis.
• Pressure regulation of soluble cytochromes c in a deep-sea piezophilic bacterium, Shewanella violacea

  M Yamada; K Nakasone; H Tamegai; C Kato; R Usami; K Horikoshi

  JOURNAL OF BACTERIOLOGY AMER SOC MICROBIOLOGY 182 (10) 2945 - 2952 0021-9193 2000/05 

  Two c-type cytochromes from the soluble fraction of a deep-sea moderately piezophilic bacterium, Shewanella violacea, were purified and characterized, and the genes coding for these cytochromes were cloned and sequenced. One of the cytochromes, designated cytochrome c(A), was found to have a molecular mass of approximately 8.3 kDa, and it contained one heme c per molecule. The other, designated cytochrome c(B), was found to have a molecular mass of approximately 23 kDa, and it contained two heme c molecules per protein molecule. The amount of cytochrome c(B) expressed in cells grown at high hydrostatic pressure (50 MPa) was less than that in cells grown at atmospheric pressure, whereas cytochrome c(A) was constitutively expressed under all pressure conditions examined. The results of Northern blotting analysis were consistent with the above-mentioned observations and suggested that the pressure regulation of cytochrome c(B) gene expression occurred at the transcriptional level. These results suggest that the components of the respiratory chain of moderately piezophilic S. violacea could be exchanged according to the growth pressure conditions.
• Structural analysis of the ntrBC genes of deep-sea piezophilic Shewanella violacea

  A Ikegami; K Nakasone; C Kato; R Usami; K Horikoshi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 64 (4) 915 - 918 0916-8451 2000/04 

  The ntrBC genes coding for the bacterial signal-transducing protein NtrB and the bacterial enhancer-binding protein NtrC of deep-sea piezophilic Shewanella violacea were cloned and their nucleotide sequences were analyzed. The conserved regions of NtrB and those of NtrC are well conserved in the case of the ntrBC products of S. violacea.
• Cloning and characterization of the gene encoding RNA polymerase sigma factor sigma(54) of deep-sea piezophilic Shewanella violacea

  A Ikegami; K Nakasone; M Fujita; S Fujii; C Kato; R Usami; K Horikoshi

  BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION ELSEVIER SCIENCE BV 1491 (1-3) 315 - 320 0167-4781 2000/04 

  We have recently reported that a sigma(54)-like factor recognizes a DNA element, designated as region A, upstream of a pressure-regulated operon in piezophilic Shewanella violacea strain DSS12 (Nakasone et al., FEMS Microbiology Lett. 176 (1999) 351-356). In this study, we isolated and characterized the rpoN gene of this piezophilic bacterium. The rpoN gene was found to encode a putative protein consisting of 492 amino acid residues with a predicted molecular mass of 55 359 Da. Significant homology was evident comparing the rpoN sequence of S. violacea with that of Escherichia coli (62.8% identity), Vibrio anguillarum (61.7% identity) and Pseudomonas putida (57.0% identity). The DNA-binding domain at the C-terminus of sigma(54) is well conserved in the case of the S. violacea rpoN gene product and the helix-turn-helix motif and the RpoN box are also present. In addition, the conserved glutamine-rich domain is present at the N-terminus. sigma(54) in S. violacea was expressed at a relatively constant level under various growth conditions as determined by both primer extension and Western blotting analyses. By means of a recombinant plasmid, a hexahistidine-tagged derivative of the sigma(54) from strain DSS12 was overexpressed in Escherichia coli and purified to near homogeneity. An electrophoretic mobility shift assay demonstrated that the purified sigma(54) protein specifically recognizes region A in the above-mentioned pressure-regulated operon. (C) 2000 Elsevier Science B.V. All rights reserved.
• The Structure of Chicken Ribosomal Protein L30

  K. Nakasone; N. Kenmochi; T. Seikichi; T. Tanaka

  Ryukyu Medical Journal 琉球医学会 14 (1) 43 - 48 1346-888X 1994/01 [Refereed]
   

  The primary structure of the chicken ribosomal protein L30 was deduced from the sequence of nucleotide in a recombinant cDNA. Chicken ribosomal protein L30 has 115 amino acids and a molecular weight of 12,814 including initiator methionine. Hybridization of the cCDNA to the restriction enzyme digests of chicken liver DNA suggests that L30 gene is a single copy. Chicken L30 is homologous to ribosomal proteins from other eukaryotes and archaebacteria but not to those from eubacteria.
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• The structure of the gene encoding chicken ribosomal protein L30

  K. Nakasone; N. Kenmochi; S. Toku; T. Tanaka

  Biochim. Biophys. Acta 18 (1174(1)) 75 - 78 1993/07 [Refereed]
  

Conference Activities & Talks

• 実験，実習をベースとした生物工学/化学の導入・動機づけ教育への取り組み－平成15年度文部科学省サイエンス・パートナーシップ・プログラム報告－  [Not invited]

  白石 浩平; 鈴木 克之; 仲宗根 薫; 岡田 芳治

  平成17年度（第53回）日本工学教育協会  2005/09  広島  平成17年度（第53回）日本工学教育協会
   

  平成15年度文部科学省サイエンス・パートナーシッププログラム採択事業についての研究成果を報告した。 動機付け教育として実験、実習が効果的であり、生徒・学生の学習活動が継続的に行なわれれば、自学自習によって高度な教育が可能であることが示唆された。同時に実験・実習内容の精査やプログラム以前での実験技術に拘わる事前実習やティーティングアシスタントの養成が重要であることを指摘した。
• Structural analysis of the nitrogen fixation-related genes from Paenibacillus ozotofixans  [Not invited]

  鈴木 克之; 坂本秀樹; 西紋郁; 仲宗根 薫; 寺岡孝敏

  平成14年度日本生物工学会大会（大阪）  2002/10  平成14年度日本生物工学会大会（大阪）
   

  窒素固定菌Paenibacillus azotofixansのニトロゲナーゼ鉄タンパク質をコードする遺伝子nifHを含む2つのオペロンのクローニングを行い、塩基配列の決定によりそれら遺伝子群の構造を明らかにした。
• 深海由来好冷好圧性細菌 Shewanella violacea のリボソーム関連遺伝子のゲノム解析  [Not invited]

  仲宗根 薫; 吉岡孝文; 木村明日香

  2002 年度日本放線菌学会大会 （つくば）  2002/05  2002 年度日本放線菌学会大会 （つくば）
   

  全ゲノム解析が進行している深海微生物 Shewanella violacea のリボソーム関連遺伝子群の構造解析を行い、 報告した。
• 高度好塩古細菌 Haloarcula japonica の rrn オペロンの解析  [Not invited]

  仲宗根 薫; 吉岡孝文; 木村明日香

  2002 年度日本放線菌学会大会 （つくば）  2002/05  2002 年度日本放線菌学会大会 （つくば）
   

  高度好塩古細菌 Haloarcula japonica の 16SrDNA のコピー数と多型性に関する系統解析を行い、 Haloarcula 属の進化について考察した。
• 深海由来好冷好圧性細菌 Shewanella violacea DSS12 株のゲノム解析  [Not invited]

  仲宗根 薫; 青野英司; 馬場知哉

  第 4 回ワークショップ微生物ゲノム研究フロンティア （千葉）  2002  第 4 回ワークショップ微生物ゲノム研究フロンティア （千葉）
   

  深海微生物 Shewanella violacea の全ゲノム解析による応用の可能性について、 特に環境修復技術開発の観点から論じた。
• Stractural analysis of the nitrogen fixation related genes from Paenibacillus azotofixans  [Not invited]

  鈴木 克之; 小澤淳信; 仲宗根 薫; 寺岡孝敏

  第 24 回日本分子生物学会年会 （横浜）  2001/12  第 24 回日本分子生物学会年会 （横浜）
   

  窒素固定菌 Paenibacillus azotofixans （IFO16645） のニトロゲナーゼ鉄タンパク質をコードする遺伝子 niflt を含む 2 つのオペロンのクローニングを行い、 それら遺伝子群の構造を明らかにした。
• Reconstitution of recombinant RNA polymerase of piezophilic Shewanella violacea  [Not invited]

  仲宗根 薫; 加藤千明; 池上昭彦; 河野広朗; 宇佐美論; 掘越弘毅

  The 6th International Symposium on Bio Nanoelectronics （川越）  2001/11  The 6th International Symposium on Bio Nanoelectronics （川越）
   

  高圧下における転写メカニズムを明らかにする目的で、深海由来好圧性細菌より転写酵素 RNA ポリメラーゼをコードする遺伝子をクローン化し、それらを試験管内にて蛋白合成を行い、本酵素の再構成を試み、それに成功した。
• 深海由来好塩性細菌Bacillus sp. HTE831株のゲノム解析(共著)  [Not invited]

  第23回日本分子生物学会年会  2000

Works

• 「有用酵素を生産する深海微生物並びにその生産する酵素」

MISC

• Newly identified sigma factors of RNA Polymerase from deep-sea bacterium Shewanella violacea

  NAKASONE Kaoru; KATO Chiaki; KAWANO Hiroaki  近畿大学工学部研究報告  (44)  15  -20  2010
• Structure analysis of BasS and BasR in rice seedling blight pathogen Burkholderia plantarii

  Yamano Yumiko; Nakasone Kaoru  近畿大学工学部研究報告  (43)  31  -35  2009
• Amino acid sequence diversities in TBP, TATA binding protein, of extremely halophilic archaeon Haloarcula japonica strain TR-1

  Matsumi Hironari; Nakasone Kaoru  近畿大学工学部研究報告  (42)  17  -20  2008
• Molecular cloning and structural analyses of the pyrF gene for orotate monophosphate (OMP) decarboxylase from extremely halophilic archaeon Haloarcula japonica strain TR-1

  Maeda Yutaka; Nakasone Kaoru  近畿大学工学部研究報告  (42)  13  -16  2008
• 3.Overexpression of RNA polymerase subunits in extremely halophilic archaeon, Haloarcula japonica and its subunit-subunit interaction analyses

  MATSUMI Hironari; SHIMOURA Yosuke; NAKASONE Kaoru  Research reports of the Faculty of Engineering, Kinki University  41-  17  -21  2007
• 2.Construction and genetic analyses of mutant for rrnB operon in extremely halophilic archaeon, Haloarcula japonica

  MAEDA Yutaka; NAKASONE Kaoru  Research reports of the Faculty of Engineering, Kinki University  41-  11  -15  2007
• Isolation and characterization of three protease-producing bacteria from a deep-sea mud of Japan Sea

  Fukumoto Takehiro; Ueki Shunichi; Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  40-  17  -20  2006/12
• Construction of plasmids for gene disruption of two chromosomal rrn operons in Haloarcula japonica strain TR-1

  Maeda Yutaka; Tominaga Daisuke; Ueki Shunichi; Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  40-  9  -12  2006/12
• Isolation and characterization of the genes for RNA polymerase subunits from Haloarcula japonica strain TR-1

  Matsumi Hironari; Amimoto Masaya; Maeda Yutaka; Fukumoto Hiroaki; Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  40-  13  -16  2006/12
• Structural analysis of S10-spc operons encoding genes for ribosomal proteins in Haloarcula japonica strain TR-1

  Nakasone Kaoru; Matsumi Hironari; Maeda Yutaka  Research reports of the Faculty of Engineering, Kinki University  40-  21  -24  2006/12
• High pressure-adapted proteins from deep-sea microorganism

  仲宗根 薫; 赤坂 一之; 加藤 千明  近畿大学工業技術研究所研究報告書  6-  7  -12  2006
• Structural analysis of gene cluster encoding acetyl-CoA carboxylase from a deep-sea piezo- and psychrophilic bacterium, Shewanella violacea strain DSS 12

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  39-  15  -18  2005/12
• Gene expression and genome analysis of high pressure-adapted marine bacterium, Piezophilic Shewanella violacea

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  39-  23  -26  2005/12
• DNA microarray analysis of high pressure effects in Escherichia coli

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  39-  19  -22  2005/12
• Piezoregulation of cytochrome bd biosynthesis in deep-sea bacterium Shewanella violacea DSS 12

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  39-  11  -14  2005/12
• Probing deep-sea genome resources based on functional genomics in laboratory model

  NAKASONE Kaoru  Bioscience & industry  63-  (1)  31  -35  2005/01
• Expression analysis groE gene under high pressure conditions from deep-sea piezophilic Shewanella violacea

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  38-  19  -22  2004/12
• Reconstitution of RNA polymerase from deep-sea piezophilic Shewanella violacea

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  38-  31  -34  2004/12
• Expression analysis dnaJ and dnaK gene under high pressure conditions from deep-sea piezophilic Shewanella violacea

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  38-  23  -26  2004/12
• Structural analysis of rpoBC gene for RNA polymerase subunit from deep-sea piezophilic Shewanella violacea

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  38-  27  -30  2004/12
• 深海微生物蛋白プロファイル作成の試み

  仲宗根 薫; 加藤 千明  近畿大学工業技術研究所研究報告書  4-  11  -14  2004
• Purification of RNA polymerase from deep-sea bacterium

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  37-  13  -18  2003/12
• Cloning of the rpoZ gene encoding RNA polymerase ω subunit from a deep-sea piezophilic bacterium

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  37-  33  -41  2003/12
• The effect of hydrostatic pressure on activity of RNA polymerase from piezophile by in vitro transcription

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  37-  19  -24  2003/12
• Structural analysis of gene cluster for EPA (eicosapentanoic acid) synthesis in deep-sea bacterium

  Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  37-  25  -31  2003/12
• Improvement in the safety of the RFHR 2-D PAGE device and its application in proteome analysis of extremophile

  Hirofuji Takao; Yamamoto Kazuhiko; Nakasone Kaoru  Research reports of the Faculty of Engineering, Kinki University  37-  43  -48  2003/12
• 深海微生物の全ゲノム解析

  仲宗根 薫  遺伝子医学  7-  (3)  436  -441  2003/09
• Isolation and Characterization of the dcw Cluster from the Piezophilic Deep-Sea Bacterium Shewanella violacea

  ISHII Akihiro; NAKASONE Kaoru; SATO Takako; WACHI Masaaki; SUGAI Motoyuki; NEGAI Kazuo; KATO Chiaki  Journal of Biochemistry  132-  (2)  183  -188  2002/08
• Genome analysis of psychrophilic and piezophilic microorganiasm

  NAKASONE Kaoru  バイオサイエンスとインダストリー = Bioscience & industry  60-  (7)  38  -39  2002/07
• Cloning and sequencing of the endA gene encoding deoxyribonuclease from a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12

  Nakasone Kaoru; Kato Chiaki; Horikoshi Koki  Technical reports of Japan Marine Science and Technology Center  (45)  63  -68  2002/03
• 深海由来好冷好圧性微生物の全ゲノム解析報告書

  仲宗根 薫; 森 浩禎; 加藤 千明  近畿大学工業技術研究所研究報告書  2-  80  -82  2002
• Structural analysis of rrn operon in deep-sea psychrophilic and piezophilic bacterium, Shewanella violacea

  木村 明日香; 加藤 千明; 仲宗根 薫  近畿大学工学部研究報告  (36)  25  -29  2002
• Marine Microbiology. Deep-sea adaptations in"Structure and Function of Biological Systems under Extreme Conditions, Pressure and Temperature Effects"(共著)

  Chiaki Kato; Lina Li; Yuichi Nogi; Kaoru Nakasone; Douglas H. Ba  Springer-Verlag, Heidelberg, Germany  205  -220  2002
• Genome analysis of extremophiles

  TAKAMI Hideto; TAKAKI Yoshihiro; LU Jei; TSUDOME Mikoko; OIDA Hanako; NAKASONE Kaoru  日本生物工学会大会講演要旨集  13-  67  -67  2001
• Cloning and sequencing of the ftsZ gene from a deep-sea piezophilic bacterium,(Shewanella violacea strain DSS12)

  Ishii Akihiro; Nakasone Kaoru; Kato Chiaki  Technical reports of Japan Marine Science and Technology Center  (42)  67  -73  2000/10
• 深海微生物の多様性と生理的特性 (総特集 海洋微生物) -- (2章 海洋微生物の生理)

  加藤 千明; 仲宗根 薫  号外海洋  (23)  38  -44  2000
• 深海由来好塩性細菌Bacillus sp. HTE831株のゲノム解析(共著)

  仲宗根 薫  第23回日本分子生物学会年会  2000
• 好アルカリ性Bacillus halodurans C-125株のシグマ因子をコードする遺伝子の解析(共著)

  仲宗根 薫  日本農芸化学会2000年度大会  2000
• 深海微生物の多様性と生理学的特性(共著)

  加藤 千明; 仲宗根 薫  月刊海洋  印刷中-  2000  [Refereed][Invited]
  
• Genome analysis of the deep-sea halophilic Bacillus sp. strain HTE831.(共著)

  Kaoru Nakasone; Hideto Takami  The 3^rd International Conference on Extremophiles  2000
• Cloning of the rpoD gene encoding the principal sigma factor of deep-sea barophilic Shewanella violacea strain DSS12.(共著)

  藤井 真介; 仲宗根 薫; 堀越 弘毅  JAMSTECR.  40-  (40)  21  -23  2000
• Cloning, sequence analysis and expression of the sigma factor σ⁵⁴(rpoN)of deep-sea barophilic Shewanella violacea strain DSS12.(共著)

  池上 昭彦; 仲宗根 薫; 藤田 昌也  JAMSTECR  40-  (40)  25  -30  2000
• Cloning and characterization of the rpoA gene encoding α subunit of RNA polymerase from deep-sea barophilic Shewanella violacea strain DSS12.(共著)

  仲宗根 薫; 加藤 千明; 堀越 弘毅  JAMSTECR  40-  (40)  15  -19  2000
• Cloning and expression of rpoD gene encoding for principal sigma subunit from a deep-sea piezophilic bacterium Shewanella violacea.(共著)

  Kaoru Nakasone  Biosci. Biotech. Biochem.  in press-  2000
• Chapter 19 How do deep-sea microorganisms respond to changes in environmental pressure?

  Chiaki Kato; Kaoru Nakasone; Mohammad Hassan Qureshi; Koki Horikoshi  Cell and Molecular Response to Stress  1-  (C)  277  -291  2000
• Cloning of two cold shock genes, cspA and cspG, from the deep-sea psychrophilic bacterium Shewanella violacea strain DSS12

  S Fujii; K Nakasone; K Horikoshi  FEMS MICROBIOLOGY LETTERS  178-  (1)  123  -128  1999/09
• Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125

  H Takami; Y Takaki; K Nakasone; T Sakiyama; G Maeno; R Sasaki; C Hirama; F Fuji; N Masui  EXTREMOPHILES  3-  (3)  227  -233  1999/08
• Analysis of cis-elements upstream of the pressure-regulated operon in the deep-sea barophilic bacterium Shewanella violacea strain DSS12

  K Nakasone; A Ikegami; C Kato; R Usami; K Horikoshi  FEMS MICROBIOLOGY LETTERS  176-  (2)  351  -356  1999/07
• Replication origin region of the chromosome of alkaliphilic Bacillus halodurans C-125

  H Takami; N Masui; K Nakasone; K Horikoshi  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY  63-  (6)  1134  -1137  1999/06
• Sequence analysis of a 32-kb region including the major ribosomal protein gene clusters from alkaliphilic Bacillus sp. strain C-125

  H Takami; Y Takaki; K Nakasone; C Hirama; A Inoue; K Horikoshi  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY  63-  (2)  452  -455  1999/02
• Chemosynthetic community at North Knoll, Iheya Ridge, Okinawa Trough.(共著)

  Tomoko Yamamoto; Kaoru Nakasone  JAMSTEC J. Deep Sea Res.  15-  19  -24  1999
• An expression of a cold shock inducible gene CspA under hydrostatic pressure in deep-sea barophilic bacterium, Shewanella sp strain DSS12

  S Fujii; K Nakasone; K Horikoshi  ADVANCES IN HIGH PRESSURE BIOSCIENCE AND BIOTECHNOLOGY  21  -24  1999
• Sequencing of three lambda clones from the genome of alkaliphilic Bacillus sp. strain C-125

  H Takami; K Nakasone; N Ogasawara; C Hirama; Y Nakamura; N Masui; F Fuji; Y Takaki; A Inoue; K Horikoshi  EXTREMOPHILES  3-  (1)  29  -34  1999/01
• An improved physical and genetic map of the genome of alkaliphilic Bacillus sp. C-125

  H Takami; K Nakasone; C Hirama; Y Takaki; N Masui; F Fuji; Y Nakamura; A Inoue  EXTREMOPHILES  3-  (1)  21  -28  1999/01
• Structural analysis of the genes encoding RNA polymerase subunits from alkaliphilic, Bacillus sp. strain C-125

  TAKAKI Yoshihiro; NAKASONE Kaoru; HIRAMA Chie; TAKAMI Hideto; INOUE Akira; TSUJII Kaoru; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  231  -231  1998/12
• Genomic analysis of barophilic bacteria isolated from deep-sea[2] -Construction and sequence analysis of NotI linking clones in Shewanella violacea-

  SMORAWINSKA Maria; LI Lina; KATO Chiaki; YANAGIBAYASHI Miki; NAKASONE Kaoru; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  232  -232  1998/12
• Genomic analysis of barophilic bacteria isolated from deep-sea[1] -Genomic analysis of rrn operons using the homing endonuclease I-CeuI in deep-sea bacteria-

  SMORAWINSKA Maria; LI Lina; KATO Chiaki; NAKASONE Kaoru; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  231  -231  1998/12
• Molecular Cloning, sequencing and purification of quinol oxidase from a deep-sea bacterium, Shewqnella violacea

  QURESHI Mohammad Hassan; YAMADA Mitsunori; NAKASONE Kaoru; KATO Chiaki; USAMI Ron; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  390  -390  1998/12
• Analysis of the pressure regulated operon in deep-sea barophilic Shewnella violacea strain DSS12.

  IKEGAMI Ahihiko; NAKASONE Kaoru; KATO Chiaki; USAMI Ron; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  389  -389  1998/12
• Cloning and structural analysis of two genes encoding different types of Cytochrome c from Deep-sea Modelately Barophilic Bacterium, Shewqnella violacea strain DSS12

  YAMADA Mitsunori; NAKASOME Kaoru; TAKAKI Yoshihiro; USAMI Ron; KATO Chiaki; HORIKOSHI Koki  日本分子生物学会年会プログラム・講演要旨集  21-  390  -390  1998/12
• Cloning and expression of the gene encoding RNA polymerase alpha subunit from alkaliphilic Bacillus sp. strain C-125

  K Nakasone; Y Takaki; H Takami; A Inoue; K Horikoshi  FEMS MICROBIOLOGY LETTERS  168-  (2)  269  -276  1998/11
• Mechanisms of gene expression controlled by pressure in deep-sea microorganisms

  K Nakasone; A Ikegami; C Kato; R Usami; K Horikoshi  EXTREMOPHILES  2-  (3)  149  -154  1998/08
• 学会見聞記(共著)

  仲宗根 薫  第5回アジア転写会議、蛋白質 核酸 酵素  (8)  84  -86  1998
• Analysis of the Upstream Element of the Pressure-regulated Operon from Deep-sea Barotolerant Shewanella sp. DSS12

  K. Nakasone; A. Ikegami; C. Kato; K. Horikoshi  Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu  7-  1318  -1320  1998
• Analysis of the Upstream Element of the Pressure-regulated Operon from Deep-sea Barotolerant Shewanella sp. DSS12

  K. Nakasone; A. Ikegami; C. Kato; K. Horikoshi  Review of High Pressure Science and Technology/Koatsuryoku No Kagaku To Gijutsu  7-  1318  -1320  1998
• Molecular cloning of the gene encoding RNA polymerase alpha subunit from deep-sea barophilic bacterium

  K Nakasone; C Kato; K Horikoshi  BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION  1308-  (2)  107  -110  1996/08
• 鶏リボソーム蛋白L30遺伝子の構造(共著)

  仲宗根 薫; 徳誠吉; 田中龍夫  第15回日本分子生物学会年会  1992

Awards & Honors

• 2003/12 極限環境微生物学会 奨励賞
   

  受賞者: 仲宗根 薫

Research Grants & Projects

• 高圧力で"調べ隊"：深海の高圧力に耐える生物を見つけよう！

  （独）日本学術振興会：2023年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI

  Date (from‐to) : 2023/04 -2024/03
• イネ苗立枯れ病を防除するための、環境に優しい新規な物質の探索

  公益財団法人サタケ技術振興財団：

  Date (from‐to) : 2022/04 -2023/03 

  Author : 仲宗根 薫
• 深海微生物探検隊；-高圧力に耐える微生物の不思議ｰ

  （独）日本学術振興会：2022年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI

  Date (from‐to) : 2022/04 -2023/03 

  Author : 仲宗根 薫
• 作って観よう!「自"作"顕微鏡で"観"る 香る発酵微生物の秘密」

  （独）日本学術振興会：2021年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI

  Date (from‐to) : 2021/04 -2022/03 

  Author : 仲宗根 薫
• 作って観よう！「自"作"顕微鏡で"観"る 香る発酵微生物の秘密」

  （独）日本学術振興会：2020年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI

  Date (from‐to) : 2020/04 -2021/03 

  Author : 仲宗根 薫
• Characterization of phenolic acid decarboxylase (PAD) in black fungi and breeding in terms of awamori aging

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

  Date (from‐to) : 2015/04 -2018/03 

  Author : Nakasone Kaoru

   

  Awamori is distilled liquor made from Indica-rice in Okinawa, and Aspergillus luchuensis and Saccharomyces cerevisiae are involved. Awamori flavors is characterized by vanillin generated from 4-vinylguaiacol (4-VG). In the fermentation, the microbial phenolic acid decarboxylase decarboxylates ferulic acid with production of 4-VG. Thus, the enzyme are important for the awamori flavors and the sources of the enzyme were unclear. In this study, the gene was detected on the genome of A. luchuensis. Besides two microbes, importance of other microbes such as lactic acid bacteria as a contaminant in the moromi are suggested, because the awamori brewery is an open system from outside. A lactic acid bacterium was isolated from the moromi, and the padC gene was also detected, suggesting both A. luchuensis and the bacterium may contribute the production of the flavor of 4-VG and vanillin. Biochemical characterization of these enzymes are carried out for future application of the flavor.
• 深海微生物由来タンパク質を利用した高圧・低温耐性ナノデバイスの創製

  独立行政法人科学技術振興機構：重点地域研究開発推進プログラム 平成19年度「シーズ発掘試験」

  Date (from‐to) : 2007/04 -2008/03 

  Author : 仲宗根 薫
• エイコサペンタエン酸(EPA)を含む、商品的付加価値を高めた納豆の作製

  公益財団法人サタケ技術振興財団：

  Date (from‐to) : 2006/04 -2007/03 

  Author : 仲宗根 薫
• 高度好塩古細菌リボソームの多型性とその分子解剖〜好塩微生物に学ぶ蛋白合成装置の進化〜

  (財)ソルトサイエンス研究財団：(財)ソルトサイエンス研究財団研究助成

  Date (from‐to) : 2005/04 -2006/03 

  Author : 仲宗根 薫
• Studies on probing genome resources and its application from deep-sea microorganism by post-genomic approaches

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

  Date (from‐to) : 2005 -2006 

  Author : NAKASONE Kaoru

   

  In this research funding area, researches on probing several gene and genome resources and its industrial applications by post-genomic approaches were carried out, using deep-sea isolate, Shewanella violacea strain DSS 12 which is already sequenced whole genome (gene candidates ; 4300). Several studies using deep-sea isolate, S. violacea strain DSS12 were included such as 1) development of a software searching efficiently upstream promoters interested in from genome sequence, 2) construction of gene database for several industrially useful enzymes, 3) biochemical analyses of alkaline phosphatases useful in the field of genetic engineering, in terms of high pressure and low temperature. The software is being developed in Perl language, and uses Tk modules for the user interface. It was initially developed to look for high-pressure-response sigma 54 promoter sequences in S. violacea genome, using a fuzzy search algorithm, but it can be used to find other promoter sequences such as sigma 32 or sigma 70 promoter sequences, or for example NtrC binding sites. The database for several industrially useful enzymes will be opened after publication of the genome analyses. In biochemical analyses of alkaline phosphatases, three enzymes whose gene is independently distributed on the genome and have different homology each other, were expressed in E. coli after construction expression plasmids. Each enzyme purified has different optimal temperature in dephosphrylation activity, showing its diversity of the enzymes and suggesting adaptation to deep-sea environments. Trying to isolate industrially useful new deep-sea microbes from deep-sea mud of Japan Sea, was also performed. From the several screening procedures, some protease-producing and amylase producing bacteria were isolated. These were suggested to be psychrotolerant isolates. Moreover, spore-forming nitrogen-fixing bacterium (Genus Paenibacillus), first example from deep-sea environment, was isolated. Phylogenetic analyses based on 16S rDNA of these isolates, showed that several bacteria seem to be new species.
• 抗菌材料としての可視光応答性酸化チタン光触媒の利用に関する基礎的研究

  (財)古川技術振興財団：

  Date (from‐to) : 2003/04 -2004/03 

  Author : 仲宗根 薫
• 深海由来好冷好圧性細菌のエイコサペンタエン酸(EPA)生産に関する基礎的研究

  公益財団法人サタケ技術振興財団：

  Date (from‐to) : 2003/04 -2004/03 

  Author : 仲宗根 薫
• Transformation by Modernization of Traditional Waterfront Settlements in the Context of their Coexistence with the Aquatic Environment

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

  Date (from‐to) : 2001 -2003 

  Author : MATSUDA Hiroyuki; NAKASONE Kaoru; IHARA Tatsuhiko; OMORI Toyuhiro; HASHIMOTO Seiyu; TOHIGUCHI Mamoru

   

  Raft houses, similar to other human settlements, form a water-based community with distinguish characteristics. The physical features of the raft houses. There are many roof styles such as gable, manila or hip. Wall types are also varied. Originally they were made of different natural lightweight materials such as woven bamboo mats or acrew pines. The wall can be made as a lifted up panel or a sliding partition wall, which can be removed and stored away. However, the major difference between the raft house and the traditional Thai house is the foundation. The raft house has no pillar. It sits on a raft supports the whole weight of the house. There are two kinds of raft : Luffashaped rafts and Rectangular pontoon. Moreover, the raft house structure is not tightly fixed together. Economic aspects of the raft house are also analyzed. The inner part of the houses is generally used for living and sleeping while the outer part is used as commercial apace like a conventional shop-house with removable walls and panels. Social futures of the raft house are also revealed. The raft house community is based on self-reliance, sharing labour, affordability sustainability and community cohesiveness. Moreover, inhabitants have closely associated with water. Watercourses are used for bathing, washing and watering plantations. The study also finds the coexistence among the raft houses, water environment and urban-rural activities in various locations. In Pitsanulok, the location of the raft house settlement is in the urban area. The inhabitants have changed their life style to suit the environment. They are no longer working in agricultural sector.New careers such as general service worker or land-baced vendor are more preferable. The raft house settlement is affected by this phenomenon. It is gradually disappearing to give way to urban modernization.
• 微生物への利用を目的としたときの可視光応答型酸化チタン光触媒のポテンシャリティ

  日本学術振興会：科学研究費助成事業 特定領域研究

  Date (from‐to) : 2002 -2002 

  Author : 井原 辰彦; 仲宗根 薫; 鈴木 克之; 白石 浩平

   

  光触媒の抗菌作用については多くの研究例が報告されているが,いずれも光触媒を機能させるためには紫外線しか利用できないので,抗菌作用について紫外線の効果と光触媒の効果とを区別して議論することは不可能であった。本研究では我々のグループが開発した可視光応答性酸化チタン光触媒を用いることにより,上記の問題点の解決を図った。検討項目は以下の3項目である。 (1)微生物の影響を調査するのに好ましい光触媒の薄膜化 (2)原核微生物として大腸菌を対象に可視光-光触媒の効果を確認 (3)真核微生物として酵母を対象に可視光-光触媒の効果を確認 (1)については,ゾルゲル法をベースとする方法で可視光応答性酸化チタン光触媒の薄膜化に成功した。具体的には,硫酸チタンを原料としてアンモニアで加水分解後,過酸化水素を加えて加水分解物を解コウして得た過酸化チタン前駆体ゾルを得た。薄膜は前駆体ゾルにアンモニアを加え,パイレックスガラス基材表面にスピンコーターでコーティングし,乾燥後,350℃の温度で1時間焼成することで成膜した。(特許出願) (2)可視光応答型酸化チタン光触媒をコーティングしたパイレックスガラス基材表面に大腸菌(E.coli from)を含む水滴を滴下し,4℃で水分が蒸発しない条件で青色および緑色LEDを照射し,光照射による影響を生菌数によって評価した。その結果,LEDを照射した場合のcell numberは,青色LEDでは0.12×10^2,緑色LEDでは0.23×10^2,と光を照射しないときの4.13×10^2と比較すると明らかに減少し,光触媒の効果が確認された。(3)酵母を対象に(2)と同様の実験を行ったところ,光触媒の効果はほとんど見られなかったことから,光触媒の作用は原核細胞には有効であるが,真核細胞には効果は薄いことが示唆された。

Teaching Experience

Fermentation ChemistryFermentation Chemistry Oita University
Web DesignWeb Design Kindai University
Practice in BiotechnologyPractice in Biotechnology Kindai University
Fundamental Experiments in BiotechnologyFundamental Experiments in Biotechnology Kindai University
Applied MicrobiologyApplied Microbiology Kindai University
Special Lecture in Applied ChemistrySpecial Lecture in Applied Chemistry Oita University

Other link

researchmap

https://researchmap.jp/nakasonek