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AKITA Motomu

    Department of Biotechnological Science　Professor
Last Updated :2024/04/25

Researcher Information

Degree

  • (BLANK)（University of Tsukuba）
  • (BLANK)（University of Tsukuba）

J-Global ID

Research Interests

  • 応用植物分子生物学   植物組織培養   応用微生物学・応用生物化学   Applied Plant Molecular Biology   Plant Tissue Culture   Applied Microbiochemistry and Applied Biochemistry   

Research Areas

  • Life sciences / Applied molecular and cellular biology
  • Environmental science/Agricultural science / Agricultural environmental and information engineering
  • Life sciences / Applied biochemistry

Academic & Professional Experience

  • 2010  Dept Biotech. Sci., Kinki Univ.professor

Education

  •        - 1983  University of Tsukuba  農林学類  応用生物化学(土壌微生物学)
  •        - 1983  University of Tsukuba

Association Memberships

  • THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS   日本植物細胞分子生物学会（旧：日本植物組織培養学会）   農業情報学会   JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS   農芸化学会   

Published Papers

  • Kenji Ito; Yuki Naka; Kotaro Matsumoto; Motomu Akita
    ACS Food Science & Technology American Chemical Society (ACS) 3 (12) 2173 - 2180 2692-1944 2023/12 [Refereed]
  • Kazuya HIGASHI; Kanji ITO; Yuki NAKA; Motomu AKITA
    Shokubutsu Kankyo Kogaku Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists 35 (4) 161 - 164 1880-2028 2023/07 [Refereed]
  • A simple method for making CO2 enriched micro-environment by a fluorinated (FEP) film and its application
    Motomu Akita; Satoki Kurimoto; Yuusuke Kominami
    Memoires of Institute of Advanced Technology, Kindai University (25) 11 - 16 2020/03 [Refereed]
  • Tamura M; Tanabe M; Valkonen JPT; Akita M
    Front. Microbiol. 10 372  2019/02 [Refereed]
  • Daijiro Harada; Katsuyuki T. Yamato; Katsura Izui; Motomu Akita
    Environmental Control in Biology Biotron Institute 56 (2) 81 - 87 1883-0986 2018 [Refereed]
     
    Eleocharis vivipara is an amphibious sedge that displays C4 traits under terrestrial environments and C3 traits in submerged environments. This plant is thus potentially advantageous for screening genes indispensable to the development of C4 photosynthesis. In this study, we performed de novo transcriptome analysis of E. vivipara using its terrestrial- and submerged-type plants. By next-generation sequencing (NGS), approximately 90 and 89 million reads were yielded for the terrestrial and submerged types, respectively, and were assembled into 27,249 unigenes. Of these de novo consensus sequences, 94.5% showed similarities to database-registered sequences, and 69.4% were assigned with Gene Ontology terms. Our de novo assembled sequence data should provide a foundation for genetic analysis of the C4 photosynthetic system.
  • 明渡 絵里朱; 平田 智子; 上井 和幸; 髙木 祐子; 水野 隆文; 水野 直治; 小林 真; 小池 孝良; 大和 勝幸; 秋田 求; 泉井 桂
    Memoirs of Institute of Advanced Technology, Kindai University 近畿大学先端技術総合研究所 (21) 33 - 48 1346-8693 2016/05 [Refereed]
     
    バラ科シモツケ属のキイシモツケ（Spiraea nipponica Maxim. var. ogawae（ Nakai） Yamanaka）は、蛇紋岩地帯にて自生する和歌山県固有の植物である。県北部に位置する龍門山の山頂付近の群落は県の天然記念物に指定されている。キイシモツケは、かつては新種とされSpiraea ogawai Nakai と命名され登録されていた。しかし近年は形態学的比較に基づいて、高知県に自生するトサシモツケ（Spiraea nipponica Maxim. var. tosaensis（ Yatabe） Makino）と共に、近畿以北に自生するイワシモツケ（Spiraea nipponica Maxim.）の変種とされ、学名も上記のように改められている。本研究では、キイシモツケがトサシモツケおよびイワシモツケなどの近縁種とは顕著に異なるのかどうかを初めて分子系統分類学の立場から検討した。同時に、キイシモツケの蛇紋岩土壌との関わりについて若干の検討を行った。まず、3 種類それぞれに特徴的な葉の形態は、種子から同一の環境条件下で生育させても再現され、生来の形質であることがわかった。次に、龍門山の土壌の交換性金属イオンの元素組成を調べ、蛇紋岩土壌特有の組成をもつことが確かめられた。キイシモツケの植物体について、金属イオンの元素分析を行った結果、蛇紋岩土壌の元素の中で最も植物に対する毒性が強いとされるニッケル（Ni）が植物体内に取り込まれていることを認めた。さらにキイシモツケの種子は発芽時に3 者の中で最も強いNi 耐性を示し、若い苗では10 mmol L - 1 のNi 2 + に対しても耐性を示した。葉緑体DNA のtRNA のtrnL – trnF 領域および核ゲノムDNA の5S RNA 遺伝子とその両端のスペーサー配列を含むITS 領域の塩基配列を解析した。その結果、葉緑体のtRNA 領域（約1000 塩基対）は完全に一致し、ITS 領域（約640 塩基対）では3 種は互いに数塩基（1％以下）異なるのみであり、分子系統学的比較においてもキイシモツケはイワシモツケおよびトサシモツケと極めて近縁であることが証明された。したがって、キイシモツケは日本の近畿以北に広く分布するイワシモツケを起源とし、和歌山の限られた蛇紋岩地帯に適応して、地理的に隔絶して自生するにいたったエコタイプであると推測された。A plant called Kiishimotsuke（ in Japanese） is indigenous to Wakayama prefecture in Japan. The plant vegetates only in the three narrow mountain areas consisting of serpentine soils. The largest colony of this plant in the area of Mt. Ryumon higher than 600 m above sea level had been designated as a prefectural natural treasure since May 1971. The plant was first discovered by Y. Ogawa, a botanist in Iwade city, and registered as a new species with a name of Spiraea ogawai Nakai in 1928. However, the validity of this registration was questioned later, because there were two kinds of plants, called Iwashimotsuke and Tosashimotsuke, which were morphologically similar to Kiishimotuke except for the shape of leaves. Iwashimotsuke is vegetating in northern area than Kinki district, and Tosashimotsuke indigenously in Kochi prefecture. Thus at present Iwashimotsuke is named as Spiraea nipponica Maxim, Tosashimotsuke as S. nipponica Maxim var. tosaensis（ Yatabe） Makino, and Kiishimotsuke as S. nipponica Maxim var. ogawae （Nakai） Yamanaka. In view of recent progress of molecular phylogenetics, the conventional method was employed to examine whether Kiishimotsuke is a species intimately related to the other two plants or not. The nucleotide sequences were determined for the regions of trn L – trn F of chloroplast DNA and ITS （Internal Transcribed Spacer） of 5S RNA coding region of nuclear DNA. The results showed that the differences in the sequences were less than 1％ among the three, demonstrating their close evolutionary relationships. Thus it is reasonable to conclude that Kiishimotsuke is a variety or ecotype of S. nipponica based not only on morphological comparison but also on molecular phylogenetic analysis. Unexpectedly significant difference in the sequence of the ITS for Iwashimotsuke（ Spiraea nipponica Maxim） was found between the one published by Potter et al.（ DQ897622） and ours（ LC133174 and LC13375）. The difference remains to be elucidated. Since Kiishimotsuke is endemic to serpentine soils in Wakayama, the interaction of the plant with serpentine soils was also studied. When these three plants were raised from seeds on non-serpentine cultivation soils, all of them grew normally and the differences in the shape of the leaves were retained, indicating the shapes of leaves were not affected by the kind of soil but determined inherently. Nikkel ion is known to be one of the most toxic metal ions in serpentine soils. When the effects of increasing concentrations of Ni 2 + on the sprouting of seeds were tested, Kiishimotsuke was most tolerant among others. In the aboveground parts of Kiishimotsuke plants grown on serpentine soils of Mt. Ryumon, accumulation of Ni 2 + was confirmed and its possible significance of the plant survival was discussed.
  • HARADA Daijiro; SAKAMOTO Tomoaki; KURATA Tetsuya; YAMATO Katsuyuki; IZUI Katsura; AKITA Motomu
    BSJ-Review 7A 35 - 41 2016 [Refereed][Invited]
  • 久保 森; 杉村 暢大; 中桐 紘治; 秋田 求; 泉井 桂
    植物環境工学 27 (3) 162 - 170 2015/09 [Refereed]
  • Yusuke Tanigaki; Kenji Ito; Yoshiyuki Obuchi; Akiko Kosaka; Katsuyuki T. Yamato; Masahiro Okanami; Mikko T. Lehtonen; Jari P. T. Valkonen; Motomu Akita
    PLOS ONE PUBLIC LIBRARY SCIENCE 9 (4) 1932-6203 2014/04 [Refereed]
     
    Plant disease resistance gene (R gene)-like sequences were screened from the Physcomitrella patens genome. We found 603 kinase-like, 475 Nucleotide Binding Site (NBS)-like and 8594 Leucine Rich Repeat (LRR)-like sequences by homology searching using the respective domains of PpC24 (Accession No. BAD38895), which is a candidate kinase-NBS-LRR (kinase-NL) type R-like gene, as a reference. The positions of these domains in the genome were compared and 17 kinase-NLs were predicted. We also found four TIR-NBS-LRR (TIR-NL) sequences with homology to Arabidopsis TIR-NL (NM_001125847), but three out of the four TIR-NLs had tetratricopeptide repeats or a zinc finger domain in their predicted C-terminus. We also searched for kinase-LRR (KLR) type sequences by homology with rice OsXa21 and Arabidopsis thaliana FLS2. As a result, 16 KLRs with similarity to OsXa21 were found. In phylogenetic analysis of these 16 KLRs, PpKLR36, PpKLR39, PpKLR40, and PpKLR43 formed a cluster with OsXa21. These four PpKLRs had deduced transmembrane domain sequences and expression of all four was confirmed. We also found 14 homologs of rice OsXB3, which is known to interact with OsXa21 and is involved in signal transduction. Protein-protein interaction was observed between the four PpKLRs and at least two of the XB3 homologs in Y2H analysis.
  • Mikko T. Lehtonen; Yoshihiro Takikawa; Gunilla Ronnholm; Motomu Akita; Nisse Kalkkinen; Elina Ahola-Iivarinen; Panu Somervuo; Markku Varjosalo; Jari P. T. Valkonen
    JOURNAL OF PROTEOME RESEARCH AMER CHEMICAL SOC 13 (2) 447 - 459 1535-3893 2014/02 [Refereed]
     
    Studies on extracellular proteins (ECPs) contribute to understanding of the multifunctional nature of apoplast. Unlike vascular plants (tracheophytes), little information about ECPs is available from nonvascular plants, such as mosses (bryophytes). In this study, moss plants (Physcomitrella patens) were grown in liquid culture and treated with chitosan, a water-soluble form of chitin that occurs in cell walls of fungi and insects and elicits pathogen defense in plants. ECPs released to the culture medium were compared between chitosan-treated and nontreated control cultures using quantitative mass spectrometry (Orbitrap) and 2-DELC-MS/MS. Over 400 secreted proteins were detected, of which 70% were homologous to ECPs reported in tracheophyte secretomes. Bioinformatics analyses using SignalP and SecretomeP predicted classical signal peptides for secretion (37%) or leaderless secretion (27%) for most ECPs of P. patens, but secretion of the remaining proteins (36%) could not be predicted using bioinformatics. Cultures treated with chitosan contained 72 proteins not found in untreated controls, whereas 27 proteins found in controls were not detected in chitosan-treated cultures. Pathogen defense-related proteins dominated in the secretome of P. patens, as reported in tracheophytes. These results advance knowledge on protein secretomes of plants by providing a comprehensive account of ECPs of a bryophyte.
  • TAKAYAMA SHINSAKU; UEMATSU TOSHIAKI; AKITA MOTOMU
    植物環境工学 Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists 26 (1) 25-29 (J-STAGE) - 29 1880-2028 2014 [Refereed]
     
    The effect of light condition on growth and alkaloid production in the Japanese plum-yew (Cephalotaxus harringtonia), whose natural habitat includes Numazu and Fuji-gun, Shizuoka Prefecture, was analyzed. Japanese plum-yew trees commonly grow in low light intensity conditions and the intensity is mostly less than 20％ of direct solar radiation. The growth of the trees in height and diameter seems to be positively correlated with light intensity; homoharringtonine (HHT) was abundant in the Fuji-gun trees, which were grown under relatively low light intensity, whereas the content of harringtonine (HT) was abundant in the Numazu trees, which were grown under relatively high light intensity. Involvement of light condition on alkaloid composition was further analyzed using Japanese plum-yew trees grown under high light intensity in Numazu. The results revealed that HT was abundant in branches exposed to high light intensity, whereas HHT was abundant in branches of trees grown in shaded areas with low light intensity.
  • AKITA MOTOMU; SEKI DAISUKE; MURASE HARUHIKO
    植物環境工学 Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists 26 (1) 30-34 (J-STAGE) - 34 1880-2028 2014 [Refereed]
     
    This study investigated the conditions for cryopreservation of the moss protonema (Racomitrium japonicum). An aseptic culture of R. japonicum was established from a single spore obtained from surface-sterilized sporangia. The cultured protonema showed extremely high tolerance to freezing. Even when directly transferred to －80°C without cryoprotectants, the cells remained viable one year later, and began growing shortly after direct transfer to fresh Knop medium maintained at room temperature. The growth and coloration of newly generated protonemata was affected by medium composition before and during long-term preservation. The suggested optimal medium for cryopreservation is Knop medium supplemented with sucrose (10g L－1) and trace elements (B, Mn, Zn, I, Mo, Cu and Co), with or without benzyladenine.
  • TANIGAKI YUSUKE; HAMANAKA MASATO; NISHINO YUSUKE; YAMATO YOSHIYUKI; AKITA MOTOMU
    植物環境工学 26 (3) 160-162 (J-STAGE)  1880-2028 2014 [Refereed]
  • Daijiro Harada; Masato Hamanaka; Ryoichi Tsuga; Motomu Akita
    5th IFAC Conference on Bio-Robotics 46 (4) 240 - 243 1474-6670 2013/03 [Refereed]
     
    Completely closed bags made with a gas permeable film are available for culture of two aquatic plants, Eleocharis vivipara and Sphagnum nemoreum. E. vivipara, the C3-C4 photosynthesis interconvertible plant, was successfully grown in a sugar free liquid medium and developed C3 type culms even under relatively high intensity of illumination. Growth of S. nemoreum was clearly stimulated in the bag using a sugar containing medium. The film system is promising for further study of responses to environmental factors such as intensity and wavelength of illumination.
  • HARADA DAIJIRO; IZUI KATSURA; AKITA MOTOMU
    Mem Fac Biol Oriented Sci Technol Kinki Univ (30) 49 - 59 1342-7202 2012/09 [Refereed]
  • Mikko T. Lehtonen; Motomu Akita; Wolfgang Frank; Ralf Reski; Jari P. T. Valkonen
    MOLECULAR PLANT-MICROBE INTERACTIONS AMER PHYTOPATHOLOGICAL SOC 25 (3) 363 - 371 0894-0282 2012/03 [Refereed]
     
    Production of apoplastic reactive oxygen species (ROS), or oxidative burst, is among the first responses of plants upon recognition of microorganisms. It requires peroxidase or NADPH oxidase (NOX) activity and factors maintaining cellular redox homeostasis. Here, PpTSPO1 involved in mitochondrial tetrapyrrole transport and abiotic (salt) stress tolerance was tested for its role in biotic stress in Physcomitrella patens, a nonvascular plant (moss). The fungal elicitor chitin caused an immediate oxidative burst in wild-type P. patens but not in the previously described delta Prx34 mutants lacking the chitin-responsive secreted class III peroxidase (Prx34). Oxidative burst in P. patens was associated with induction of the oxidative stress-related genes AOX, LOX7, and NOX, and also PpTSPO1. The available delta PpTSPO1 knockout mutants overexpressed AOX and LOX7 constitutively, produced 2.6-fold more ROS than wild-type P. patens, and exhibited increased sensitivity to a fungal necrotrophic pathogen and a saprophyte. These results indicate that Prx34, which is pivotal for antifungal resistance, catalyzes ROS production in P. patens, while PpTSPO1 controls redox homeostasis. The capacity of TSPO to bind harmful free heme and porphyrins and scavenge them through autophagy, as shown in Arabidopsis under abiotic stress, seems important to maintenance of the homeostasis required for efficient pathogen defense.
  • Akio Tani; Yuichiro Takai; Ikko Suzukawa; Motomu Akita; Haruhiko Murase; Kazuhide Kimbara
    PLOS ONE PUBLIC LIBRARY SCIENCE 7 (3) e33800  1932-6203 2012/03 [Refereed]
     
    Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.
  • Mikko T. Lehtonen; Eeva M. Marttinen; Motomu Akita; Jari P. T. Valkonen
    ANNALS OF APPLIED BIOLOGY WILEY-BLACKWELL 160 (3) 298 - 307 0003-4746 2012 [Refereed]
     
    Bryophytes (mosses) are non-vascular plants inhabited by a large number of fungal species, but whether mosses can act as reservoirs of fungal pathogens of crop plants has gained little attention. A few moss species including the Sunagoke moss (Racomitrium japonicum; family Grimmiaceae) are found to have modern economical applications in uses such as greening of urban environments. In a previous study, we identified fungi causing symptoms of varying severity in the commercially grown Sunagoke moss. The aim of this study was to test whether the same fungal isolates are pathogenic to vascular plants. An isolate of Fusarium avenaceum lethal to the Sunagoke moss caused root and crown rot in barley (Hordeum vulgare) and reduced germination of tomato (Solanum lycopersicum) and carrot (Daucus carota) grown in the infested soil. An isolate of Cladosporium oxysporum causing mild symptoms in moss reduced growth and caused reddening and premature death of carrot seedlings. On the other hand, isolates of Alternaria alternata and Fusarium oxysporum lethal to the Sunagoke moss caused no detectable symptoms in any tested vascular plant, suggesting specialisation of these isolates to moss. Chloroplast repositioning was observed in the neighbouring cells towards the initially infected cell following infection with F. avenaceum and A. alternata in Physcomitrella patens (family Funariaceae), a model moss used to study microscopic symptoms. Infection of P. patens with a non-virulent Apiospora montagnei isolate induced formation of papillae in the moss cells, indicating activation of host defence as described in vascular plants. Results suggest that mosses and vascular plants may be linked by a common microbial interface constituted by pathogenic fungi. The findings have epidemiological implications that have gained little previous attention.
  • TAKAYAMA SHINSAKU; MERA NOBUAKI; AKITA MOTOMU
    植物環境工学 Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists 24 (4) 224-232 (J-STAGE) - 232 1880-2028 2012 [Refereed]
     
    This paper presents an overview of the applications of plant biotechnology for industrialscale production of clonal plants, secondary metabolites and heterologous proteins. Clonal plants are produced at industrialscale tissue culture nurseries with tissue culture facilities, and these production systems have been scaled-up recently with the use of bioreactors. Plant biotechnology-based systems for production of secondary metabolites and/or heterologous proteins have been optimized in plants through various expression strategies. Recently, the production of secondary metabolites in large-scale bioreactors has been commercialized. Furthermore, heterologous proteins from various eukaryotic origins such as antigens, as well as those with vaccine value are expressed in plants. Studies on the production of a number of commercially interesting products currently being investigated are described.
  • Motomu Akita; Mikko T. Lehtonen; Hilkka Koponen; Eeva M. Marttinen; Jari P. T. Valkonen
    SCIENCE OF THE TOTAL ENVIRONMENT ELSEVIER SCIENCE BV 409 (17) 3166 - 3173 0048-9697 2011/08 [Refereed]
     
    Drought and heat tolerance of the Sunagoke moss (Racomitrium japonicum) and the low thermal conductivity of the dry moss tissue offer novel greening and insulation possibilities of roofs and walls to mitigate the heat island phenomenon in urban environments. However, damage may appear in the moss panels under humid conditions in Japan. In this study we characterized fungi associated with the damaged areas of the Sunagoke moss panels. Fungi were identified by morphology and internal transcribed spacer (ITS) sequence analysis and tested for pathogenicity on R. japonicum (Grimmiaceae) and an unrelated moss species (Physcomitrella patens; Funariaceae) under controlled conditions. Alternaria alternata, Fusarium avenaceum and Fusarium oxysporum caused severe necrosis and death, whereas Cladosporium oxysporum and Epicoccum nigrum caused milder discoloration or chlorosis in both moss species. The fungi pathogenic on moss were closely related to fungal pathogens described from cultivated vascular plants. Ammonium increased severity of fungal diseases in moss. This study demonstrated that fungi can cause economically significant diseases in cultivated moss and hamper commercial use of the moss panels unless appropriate control methods are developed. Use of a single moss clone to cover large surfaces and the air pollutants such as ammonium may increase the risk for fungal disease problems. (C) 2011 Elsevier B.V. All rights reserved.
  • Akio Tani; Motomu Akita; Haruhiko Murase; Kazuhide Kimbara
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 112 (1) 32 - 39 1389-1723 2011/07 [Refereed]
     
    The use of Racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. However, its utilization is hampered by slow growth rate. Here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. Most of the isolates belonged to Pseudomonas, Rhodococcus, and Duganella species. The isolates were biochemically characterized according to their type of interaction with plants, i.e., production of auxin, siderophores, or hydrogen cyanate, growth in the absence of an added nitrogen source, calcium phosphate solubilization, utilization of sugars, polymers, or aliphatic compounds, and antifungal activity. The isolates were applied to sterile protonemata and non-sterile adult gametophytes of R japonicum to evaluate their effect on plant growth. Furthermore, we isolated fungi that inhibited moss growth. Our results suggest that the microbial community structure in hydroponic cultures is important to stabilize moss production and the isolates that promote moss growth have potential to be utilized as biofertilizers for moss production. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
  • AKITA MOTOMU; SUZUKI TAKAHIRO
    Mem Fac Biol Oriented Sci Technol Kinki Univ 近畿大学生物理工学部 26 (26) 23 - 37 1342-7202 2010/09 [Refereed]
     
    植物工場生産を高効率化・高付加価値化するための２つのアプローチ、すなわち、植物を改変するための生物学的アプローチ、および、空間や光の効率的利用などに関する工学的アプローチの２つについて概説した。
  • Li-mei Chen; Hiroya Yurimoto; Kun-zhi Li; Izumi Orita; Motomu Akita; Nobuo Kato; Yasuyoshi Sakai; Katsura Izui
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 74 (3) 627 - 635 0916-8451 2010/03 [Refereed]
     
    Formaldehyde (HCHO) is an air pollutant suspected of being carcinogenic and a cause of sick-house syndrome. Microorganisms called methylotrophs, which can utilize reduced C-1 compounds such as methane and methanol, fix and assimilate HCHO, whereas most plants are unable to assimilate HCHO directly. We found that a bacterial formaldehyde-fixing pathway (ribulose monophosphate pathway) can be integrated as a bypass to the Calvin-Benson cycle in transgenic Arabidopsis thaliana and tobacco by genetic engineering. These plants showed enhanced tolerance to HCHO and enhanced capacity to eliminate gaseous HCHO by fixing it as a sugar phosphate. Our results provide a novel strategy for phytoremediation of HCHO pollution, and also represent the first step toward the production of plants that can assimilate natural gas-derived C-1 compounds.
  • NAGAMATSU HIROAKI; SAKAGAMI AYA; YAMAZAKI YASUHIRO; YOSHIMURA KAZUE; SEKII AI; AKITA MOTOMU; IZUI KATSURA
    Mem Fac Biol Oriented Sci Technol Kinki Univ 近畿大学生物理工学部 (25) 7 - 16 1342-7202 2010/03 [Refereed]
     
    われわれは陸生型Eleocharis viviparaのC_4光合成型ホスホエノールピルビン酸カルボキシラーゼ(PEPC)の環境応答や酵素の活性調節などの研究の過程で、このPEPCが従来から広く植物材料に対して用いられてきた、不溶性のポリビニルポリピロリドン(PVPP)を助剤とする抽出法ではほとんど抽出されないという困難に遭遇した。本研究ではこの問題を解決するため、抽出方法の検討を行った。その結果、スキムミルク (Skim milk) が助剤として非常に有効であることを見出し、PEPCの抽出効率を飛躍的に高めることに成功した。詳細な解析の結果、Skim milkの主成分であるカゼイン(Casein)がPEPC抽出に有効であることを示し、さらにCaseinよりも溶解性の高いCaseinのナトリウム塩(Casein sodium salt)の方が高い効果を示すことを見出した。Caseinの主要構成成分であるα-、β-、及びK-caseinはいずれも同程度に有効であった。通常は、PVPPを助剤として用いる場合には可溶性のポリビニルピロリドン(Polyvinylpyrrolidone, PVP)をさらに加えることはないが、われわれは偶然、PVPをPVPPと併せて用いてみたところ、抽出効率が非常に良くなることを観察し、PVPにはPVPPにはない抽出能力があることを見出した。平均分子量を異にするPVPについて検討した結果、平均分子量が約10,000と小さいものが最も有効であった。さらに、本抽出法の応用性及び新たな可能性について論じた。Departmental Bulletin Paper
  • Mikko T. Lehtonen; Motomu Akita; Nisse Kalkkinen; Elina Ahola-Iivarinen; Gunilla Ronnholm; Panu Somervuo; Mattias Thelander; Jari P. T. Valkonen
    NEW PHYTOLOGIST WILEY-BLACKWELL 183 (2) 432 - 443 0028-646X 2009 [Refereed]
     
    P>Mosses (Bryophyta) are nonvascular plants that constitute a large part of the photosynthesizing biomass and carbon storage on Earth. Little is known about how this important portion of flora maintains its health status. This study assessed whether the moss, Physcomitrella patens, responds to treatment with chitosan, a fungal cell wall-derived compound inducing defense against fungal pathogens in vascular plants. Application of chitosan to liquid culture of P. patens caused a rapid increase in peroxidase activity in the medium. For identification of the peroxidase(s), matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF)/MS, other methods and the whole-genome sequence of P. patens were utilized. Peroxidase gene knock-out mutants were made and inoculated with fungi. The peroxidase activity resulted from a single secreted class III peroxidase (Prx34) which belonged to a P. patens specific phylogenetic cluster in analysis of the 45 putative class III peroxidases of P. patens and those of Arabidopsis and rice. Saprophytic and pathogenic fungi isolated from another moss killed the Prx34 knockout mutants but did not damage wild-type P. patens. The data point out the first specific host factor that is pivotal for pathogen defense in a nonvascular plant. Furthermore, results provide conclusive evidence that class III peroxidases in plants are needed in defense against hostile invasion by fungi. New Phytologist (2009) 183:432-443doi: 10.1111/j.1469-8137.2009.02864.x.
  • AKITA MOTOMU; YAMAZAKI YASUHIRO; SAITO NAOYA; KURODA TOSHIAKI
    Mem Sch Biol Orient Sci Technol Kinki Univ 近畿大学 18 (18) 9 - 14 1342-7202 2006/09 [Refereed]
     
    Polyhedrin of Bombyx mori cytoplasmic polyhedrosis virus is a protein that crystallizes and forms polyhedral particles in insect cells infected by the virus. We introduced the polyhedrin gene to Nicotiana tabacum BY-2 cells with the GUS-fused VP3 gene, encoding the viral protein that is encapsulated in the polyhedron, by an Agrobacterium mediated transformation. The expression vector was constructed to regulate these two genes under CaMV35S promoters. A NPTII expression cassette regulated by the nopaline synthase promoter was located on the same T-DNA region. Transformed BY-2 cells that proliferated well on solid medium were examined with a microscope but no polyhedra were distinguished in the cells even if the cells were GUS-stained. Since production of polyhedrin was confirmed by a western blotting analysis, it is necessary to investigate another suitable condition for crystallization of polyhedrin and encapsulation of VP3 into the polyhedra.
  • M. Akita; K. Negishi; A. Kitano; M. Iwasaki; R. Komae; Y. Ohta; T. Kuriu; T. Takii
    PROCEEDINGS OF THE VTH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURE BREEDING, VOLS 1 AND 2 INT SOC HORTICULTURAL SCIENCE 725 (725) 579 - + 0567-7572 2006 [Refereed]
     
    A cherry (Cerasus x yedoensis Matsum.) was mass propagated using tissue culture techniques. Shoot primordia were efficiently induced on a solid MS medium containing 30 g L-1 sucrose, 0.5 mg L-1 BA, 0.1 mg L-1 IBA and 3 mg L-1 GA(3). The primordia were maintained and proliferated using the same medium or a liquid medium whose composition was the same with the solid medium except elimination of agar. The primordia were also successfully propagated in a bioreactor. Shoots were regenerated on a modified MS solid medium in which the concentration of major inorganic salts (KNO3, NH4NO3 and CaCl2) was reduced by half, and sucrose and hormone concentrations were the same as that of medium for the primordia. Root formation was clearly inhibited in this medium but it was stimulated by subculturing the plants on the medium without growth regulators. Plantlets were successfully acclimatized on 1:1 mixture of Kanuma-soil (a weathered pumice) and vermiculite. A culture scheme for efficient system for mass propagation of cherry is discussed.
  • OTA YOSHIMOTO; AKITA MOTOMU
    近畿大学生物理工学部紀要 近畿大学 15 (15) 1 - 13 1342-7202 2005/03 [Refereed]
     
    Ancient Greek physician Hippocrates (460?-377? BC), the Father of Medicine, established an oath and asked the people who intend to be a physician to take the oath. In this Hippocratic oath starting as "I swear by Apollo Physician and Asclepius and Hygieia and Panaceia and all the gods and goddesses, making them my witnesses, that I will fulfil according to my ability and judgment this oath and this covenant : ", there is a sentence saying "...I will apply dietetic measures for the benefit of the sick according to my ability and judgment ; I will keep them from harm and injustice. ..." That is to say, life is respectable and the balance of benefit resulting from any actions to life must be over harm. The oath shows that, from those olden times, the importance of ethical consideration in relation to any actions of medicine affecting human life has been recognized by medical professionals. Today, scientists have made clear the subtle architecture of life in detail at the level of molecules, and have made it possible to manipulate genes of organism. They, as ancient Greek physician did, should consider seriously from ethical point of view about the balance between benefit and harm resulting from any actions applied to lives. This is particularly important for genetically modified (GM) crops, because crops are of fundamental importance for human life and their quality may influence directly human health. An ultimate benefit -good- resulting from GM crops is to ensure food for explosively increasing world population, and the confronting harm -bad- to this is uneasiness about safety as daily food and disturbance to natural environment. Even though scientists produced GM crops aiming at only the good, the final, decision to accept them will be made by the consumer. Thus, it is quite important to offer appropriate and understandable information about GM crop to the general public for making decision. In this respect, scientists who carry out the research at university have a responsibility to consider thoroughly on safety and ethical issue of GM crops and to educate students with appropriate manner. In this review, we discuss whether genetic modification of crops can be admitted ethically by trying to answer to public concern about the safety of transgenic crops as food and on environment.
  • Robert A. Andersson; Motomu Akita; Minna Pirhonen; Elin Gammelgård; Jari P. T. Valkonen
    Journal of General Plant Pathology 71 (1) 23 - 28 1345-2630 2005/02 [Refereed]
     
    Vascular plants have various inducible resistance mechanisms as defense against pathogens. Mosses, small nonvascular plants (subkingdom Bryophyta), have been little studied in regard to their pathogens or modes of defense. Data here show that Erwinia carotovora, a bacterial plant pathogen that causes softrot in many dicotyledonous plants, can also cause soft rot symptoms in the moss Physcomitrella patens. Infection of moss by E. carotovora required pathogenicity factors similar to those required to infect vascular plants and, again as in vascular plants, salicylic acid (SA) induced moss to inhibit tissue maceration by Erwinia. These data reveal that SA-dependent defense pathways may have evolved before differentiation of vascular and nonvascular plants. © The Phytopathological Society of Japan and Springer-Verlag 2005.
  • S. Takayama; M. Akita
    Liquid Culture Systems for in vitro Plant Propagation Springer Netherlands 61 - 78 2005 [Refereed]
     
    Bioreactors are an efficient tool for the production of plant propagules but, at present, their application is commercialized in only a few tissue culture companies. The present article reviews practical aspects of the use of bioreactors in the mass propagation of plants in relation to the responses of plant propagules in liquid medium, the characteristics of bioreactor culture techniques in plant propagation and discusses case studies of the use of bioreactors for several plant species including Fragaria ananassa, Lilium species, Hippeastrum hybridum, Gladiolus grandiflorus, Spathiphyllum, Colocasia esculenta and Solanum tuberosum. The establishment of plantlets from bioreactors and future prospects are also described.
  • Moss as a model system for studies on plant-pathogen interactions
    M. Akita; R.A. Andresson; M. Pirhonen; E. Gammelgard; J.P.T.Valkonen
    Biology of Plant-Microbe Interactions 4 244 - 247 2004/12 [Refereed]
  • AKITA MOTOMU; KAJIWARA HISASHI; OTA YOSHIMOTO
    植物工場学会誌 JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS 16 (4) 215 - 219 0918-6638 2004/12 [Refereed]
     
    A simple method of genetic transformation of leaf lettuce (Lactuca sativa L.) by infection of the seeds with Agrobacterium tumefaciens was investigated. Sterilized and water-imbibed seeds were vacuum-infiltrated with a suspension of A. tumefaciens that harbored pIG121-Hm. After co-cultivation, the shoot primodium tissue was induced on a medium that contained antibiotics, for elimination of the bacteria and selection of transgenic cells. Plants regenerated on the selection medium showed GUS activity. These results indicated that transgenic leaf lettuce can be developed using the seed infiltration method.
  • LUO S P; AKITA MOTOMU; OTA YOSHIMOTO
    近畿大学生物理工学部紀要 近畿大学 12 (12) 23 - 29 1342-7202 2003/09 [Refereed]
     
    An efficient method for transformation of leaf lettuce (Lactuca sativa L.) was developed. Leaf primodia-like tissue was established from the apical meristem under rotation culture using BA containing medium. Adventitious shoots with roots were efficiently induced from the tissue. When Agrobacterium tumefaciens harboring GUS gene was infected to the primodia-like tissue, shoots were generated on a selection medium and they clearly showed GUS activity.
  • OTA YOSHIMOTO; AKITA MOTOMU
    近畿大学生物理工学部紀要 近畿大学 11 (11) 1 - 23 1342-7202 2002/12 
    The utilization of transgenic plants for production of recombinant proteins and peptides of pharmaceutical interest has gained increasing attention over past decades. Plant expression systems have many potential advantages for producing these proteins. First, production cost is low. A large amount of raw material containing the target proteins are produced on an agricultural scale. Only sun light, water and fertilizer are required. Second, purification is easy or not necessary. The proteins can be expressed and accumulated in a particular compartment such as seeds or tubers. Edible plants may be utilized, in some cases, to deliver the protein directly. Third, plants are convinced to be safe hosts. Plant do not serve as hosts for human pathogens, and plant viruses are not pathogenic to humans.In this review, we discuss recent development in "molecular farming" for the production of biopharmaceuticals, edible vaccines, antibodies, and autoantigens for treatment of autoimmune diseases in transgenic plants.
  • M Akita; JPT Valkonen
    JOURNAL OF MOLECULAR EVOLUTION SPRINGER-VERLAG 55 (5) 595 - 605 0022-2844 2002/11 [Refereed]
     
    Plant disease resistance (R) genes encode proteins in which several motifs of the nucleotide-binding region (NBS) are highly conserved. Using degenerate primers designed according to the kinase 1 (P-loop) and hydrophobic (HD) motifs of the R gene NBS domains, homologous sequences were cloned from moss (Physcomitrella patens; phylum Bryophyta) representing an ancient nonvascular plant. A novel gene family (PpC) with at least eight homologous members was found. Expression of five members was detected. The level of expression was dependent on the developmental stage of moss, being higher in the gametophyte tissue than in the protonema tissue. The PpCs contained the conserved motifs characteristic of the NBS regions of R genes, and a kinase domain was found upstream from the NBS region. Phylogenetic analysis using the deduced NBS amino acid sequences of the PpCs and the plant genes available in databanks indicated that the PpCs show the closest relationship with the TIR-NBS class of R genes. No significant similarity to plant genes other than R genes was observed. These findings shed novel light on the evolutionary history of the R gene families, suggesting that the NBS region characteristic of the TIR-NBS class of R-like genes evolved prior to the evolutionary differentiation of vascular and nonvascular plants.
  • Motomu Akita; Yoshimoto Ohta
    Plant Biotechnology 19 (5) 353 - 356 1347-6114 2002 [Refereed]
     
    Storage organ of Dioscorea opposita was efficiently generated by using a drum type bioreactor. This reactor was not equipped with a system for mechanical aeration and explants were immersed intermittently into the medium on rotation. When explants precultured on a rotary shaker were transferred to the bioreactor, approx. 230 pieces of storable organs that could be bulbils or microtubers were generated after 5 weeks. These organs sprouted within a week and eventually grew into normal plants. © 2002, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.
  • YAMADA SHIHO; AKITA MOTOMU; OTA YOSHIMOTO
    近畿大学生物理工学研究所紀要 近畿大学先端技術総合研究所 (6) 25 - 28 1344-414X 2001/06 [Refereed]
     
    Growth of root of sweet potato (Ipomoea batatas Lam) was significantly stimulated using a Ebb and Flow type culture system.
  • Conditions for efficient protoplast release in moss (Physcomitrella patens)(共著)
    M.Akita; J.P.T.Valkonen
    Memoirs of the school of Biology-Oriented Science and Technology of Kinki University 8 49 - 55 2001 [Refereed]
  • M Akita; Y Ohta
    PLANT CELL REPORTS SPRINGER VERLAG 19 (7) 705 - 709 0721-7714 2000/06 [Refereed]
     
    The effect of Tiron (disodium 1,2-dihydroxybenzene-3,5-disulfonate) on the growth, morphology and alkaloid content of adventitious roots in Atropa belladonna was investigated. High concentrations of Tiron had an inhibitory effect on growth of the root. The appearance of cultured roots was significantly changed from rough roots accompanied with callus-like tissue in control cultures to fine roots without callus formation. Alkaloid content was drastically increased by the addition of 1 mM Tiron to the medium. The influence of NAA, which has an inhibitory effect on alkaloid production, was partially restored by Tiron treatment, indicating that this radical scavenger may affect the production of alkaloids through modulation of the mode of action of auxin. Glutathione content of the root was not influenced by Tiron.
  • AKITA MOTOMU; OTA YOSHIMOTO; TAKAI GOKI
    植物工場学会誌 JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS 12 (1) 18 - 22 0918-6638 2000/03 [Refereed]
     
    The production of thymol was investigated in a bioreactor culture of plantlets of Thymus vulgaris. The plantlets successfully multiplied in a liquid shake culture, but the thymol content was quite low compared to the static culture. When the plantlets were cultured using a non-mechanical agitated type of bioreactor, the plantlets grew well and the productivity of thymol (mol/dry weight of plantlets) was the almost the same as that of the static culture. Most of thymol (approx. 80%) was contained in the medium. When an ebb and flow type of culture system (EFBR type) was used as the bioreactor, the growth was stimulated and the productivity of thymol was increased whereas the medium contained only a small amount of thymol (7% of the total thymol). The EFBR type is thought to be suitable for the multiplication of plantlets and the production of thymol.
  • AKITA MOTOMU; OTA YOSHIMOTO
    近畿大学生物理工学研究所紀要 近畿大学生物理工学研究所 (2) 33 - 37 1344-414X 1999/03 [Refereed]
     
    継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University培養由来のサトイモ貯蔵器官(球茎)の苗質を評価する目的で、培養直後の球茎の乾燥耐性および球茎中に含まれる貯蔵物質(デンプン、レクチン)の含量を測定した。培養由来の球茎は、実験室内において速やかに萎れた。貯蔵物質の含量は市販の球茎よりも著しく低かった。これらの結果から、培養由来の球茎は貯蔵器官としての発達が不十分と判断された。培養条件によって貯蔵物質の含量は影響され、その含量は順化率に関係する可能性が示唆された。 （英文） Quality of in vitro propagated corms of yam (Colocasia esculenta cv. Ishikawawase) as the seed plants was estimated. In vitro derived corms were easily wilted when they were kept under the room condition. The contents of two kinds of reserve substances, starch and lectin, were significantly less than those in the field grown corms. These results indicate that development as the storage organ is insufficient in in vitro propagation of corms. Composition of the medium influenced on the content of these reserve substances which may affect on the efficiency of acclimatization of the corms.
  • M Akita; Y Ohta
    PLANT CELL REPORTS SPRINGER VERLAG 18 (3-4) 284 - 287 0721-7714 1998/12 [Refereed]
     
    We have developed a simple system for mass propagation of plant organs using a bioreactor without forced aeration. In this system, explants were cultured in bottles equipped with an air-permeable membrane on the cap and these bottles were slowly rotated on a bottle roller. Microtubers of potato were induced using a two-step culture method. In the first step, potato plantlets were cultured under static conditions. After shoot proliferation, the culture medium was replaced with a medium containing a higher concentration of sucrose and the bottles were rotated at 1 rpm. The number of tubers was clearly increased in this system compared to the culture without rotation. The results indicated that our system can be applied for mass propagation of potato tubers at low cost.
  • Bioreactor techniques for large-scale culture of plant propagules(共著)
    高山真策; 秋田 求
    Advances in Horticultural science 12 (2) 93 - 100 1998 [Refereed]
  • M Kawamura; T Shigeoka; M Tahara; M Takami; H Ohashi; M Akita; Y Kobayashi; T Sakamoto
    SEIBUTSU-KOGAKU KAISHI-JOURNAL OF THE SOCIETY FOR FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 76 (1) 3 - 7 0919-3758 1998 [Refereed]
     
    Small cell clusters with high taxol contents were selected in the following manner. Cell suspensions derived from Taxus brevifolia and Taxus cuspidata were reacted with primary polyclonal antibodies of taxol, and then labeled by magnetic or fluorescent (FITC) secondary antibodies. Cell clusters bound to the secondary antibodies were picked up manually by a small magnet or through a fluorescence microscope. It was possible to seperate cell clusters having taxol contents of more than 1000 mu g/g DW from cell suspensions which had an average taxol content of 5 mu g/g DW pr less.
  • 秋田 求; 三輪 敬之; 今泉 清; 浦林 竜太; 高山 真策
    近畿大学生物理工学部紀要 近畿大学 1 31 - 42 1342-7202 1997/02 [Refereed]
     
    植物の分化した組織および器官を培養するための装置を開発し、Atropa belladonna(ベラドンナ)の不定根培養系を用いて、その有用性を確かめた。この新たに開発された10-L容の培養装置内では、ベラドンナ根は集塊を形成することなく良好に増殖した。一般的な通気型培養槽で培養した場合と比較し、この培養装置を用いた場合には、培養開始後72日目で、根量については約1.5倍、アルカロイド含量については約2倍に増大させることができた。
  • 太田 喜元; 秋田 求
    近畿大学環境科学研究所研究報告 近畿大学 25 133 - 134 0287-5071 1997
  • M Kawamura; T Shigeoka; M Akita; Y Kobayashi
    JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 82 (6) 618 - 619 0922-338X 1996 [Refereed]
     
    We developed an apparatus for inoculation of a large number of plantlets or plant parts such as shoots or roots into a large-scale fermenter at one time. Ab opa belladonna adventitious roots (2.4 kg FW) were inoculated into a 500 l fermenter under sterile conditions, and 35 kg FW of the roots were obtained after 60-d cultivation. There were no differences in proliferation rate and scopolamine content between a 10 l fermenter culture used as control and the 500 l fermenter culture. The success of large-scale root culture was considered to be dependent on the use of the new inoculation apparatus.
  • 秋田求
    (筑波大学・博士論文) 1996
  • AKITA M.
    Acta Hort. 440 554 - 559 1996 [Refereed]
  • S TAKAYAMA; M AKITA
    PLANT CELL TISSUE AND ORGAN CULTURE KLUWER ACADEMIC PUBL 39 (2) 147 - 156 0167-6857 1994/11 [Refereed]
     
    Plant regenerated organs such as shoots, bulbs, microtubers, corms, embryos, etc. have been successfully proliferated in the bioreactor. The use of a bioreactor leads to the development of technology suitable for large scale plant propagation. The basic construction and characteristics of various types of bioreactor systems are reviewed in relation to shoot and embryo cultures. A pilot scale 500 liter bioreactor system was applied to the production of large scale Stevia rebaudiana shoots.
  • AKITA MOTOMU; SHIGEOKA TAKEO; KOIZUMI YOKO; KAWAMURA MICHIO
    植物工場学会誌 JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS 6 (2) 113 - 121 0918-6638 1994/06 [Refereed]
     
    The shoot primordium is a suitable inoculant for large scale culture of shoots comparing with the shoot. itself. A culture method of multiple shoots starting from the shoot primordium was established. The influence of scale up conditions on the growth of multiple shoots originating from the primordia was also investigated.
    Shoot primordium of Stevia rebaudiana has been induced and maintained in modified MS liquid medium containing NAA and BA. The primordia grew constantly by about 7 times in 1 month in the liquid shake culture. The multiple shoots were then induced efficiently from the primordia by eliminating the growth hormones from the MS medium. They grew constantly by about 20 times in 1 month in the liquid shake culture. The growth of shoots in culture vessels of different sizes, 0.3 l (flask), 6 l (jar fermentor) and 500 l (bioreactor), were compared by inoculating the primordia at a fixed density of about 10 gl-1. The shoots grew actively to fill up culture vessels within a month, and the final weight of shoots per unit volume of medium was similar in each culture vessel. This result strongly suggests that multiple shoots can be mass propagated in a large scale without affecting the culture efficiency.
  • AKITA MOTOMU; KOIZUMI YOKO; KAWAMURA MICHIO
    植物工場学会誌 JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS 6 (2) 122 - 127 0918-6638 1994/06 [Refereed]
     
    Development of an efficient system for shoot mass propagation through the entrapment of shoot primordia was investigated on Stevia rebaudiana. Shoot primordia of Stevia rebaudiana could be trapped into cubes of reticulate form matrix of loofah, sponge of Luffa aegyptica, when the primordia were cultured in a rotary culture system (flask scale) or in the drum type jar fermentor system in the presence of the cubic matrices. The shoot primordia grew to form uniform clumps staying in loofah matrices and the growth was stimulated by the entrapment compared to the control culture without the matrices. Shoots were efficiently induced from the entrapped clumps of primordia under subsequent static culture condition and were acclimatized successfully without removing the matrices.
  • M AKITA; S TAKAYAMA
    PLANT CELL TISSUE AND ORGAN CULTURE KLUWER ACADEMIC PUBL 36 (2) 177 - 182 0167-6857 1994/02 [Refereed]
     
    Potato (Solanum tuberosum L.) tubers were mass propagated in a jar fermentor using a two-step method consisting of a shoot multiplication step (phase 1) followed by a tuber induction and development step (phase 2). Tuberization was observed within 2 weeks of phase 2 and the number of tubers did not increase after this culture period. In contrast, total tuber weight continuously increased for at least 10 weeks. Although the number of tubers and the total tuber weight clearly decreased under the lower temperature (17-degrees-C), this weight decrease was partially prevented by changing the temperature from 17-degrees-C to 25-degrees-C after 2 weeks of phase 2. This result indicates that tuber development can be controlled independently of induction. Lower temperatures influenced the localization and size distribution of tubers in the jar fermentor.
  • M AKITA; S TAKAYAMA
    PLANT CELL REPORTS SPRINGER VERLAG 13 (3-4) 184 - 187 0721-7714 1994/01 [Refereed]
     
    A procedure for the mass propagation of potato (Solanum tuberosum L.) tubers using a laboratory scale jar fermentor is described. Tuberization was clearly suppressed when the shoots were completely submerged in the liquid medium. Tubers were mainly formed at the medium surface. When shoots were cultured by the semi-continuous medium surface level control method, in which the medium surface level was raised or lowered throughout the culture period, tubers were induced and developed in every area in the jar fermentor. Tubers propagated by this method contained about 18 % (w/w) dry matter, slightly less than in field grown tubers, but most of the tubers weighing more than 0.2 g(FW) sprouted under room condition, without any acclimatization, during 3 month after the culture.
  • M AKITA; T SHIGEOKA; Y KOIZUMI; M KAWAMURA
    PLANT CELL REPORTS SPRINGER VERLAG 13 (3-4) 180 - 183 0721-7714 1994/01 [Refereed]
     
    A procedure for the mass propagation of multiple shoots of Stevia rebaudiana is described. Isolated shoot primordia were used as the inoculum to obtain clusters of shoot primordia. Such clusters were grown in a 500 liter bioreactor to obtain shoots. A total of 64.6 Kg of shoots were propagated from 460 g of the inoculated shoot primordia. These shoots were easily acclimatized in soil.
  • AKITA Motomu; TAKAYAMA Shinsaku
    Plant Biotechnology Japanese Society for Plant Cell and Molecular Biology 10 (3) 255 - 259 0289-5773 1993/12 [Refereed]
     
    The resting period and field performance of potato (Solanum tuberosum L.) tubers derived from a jar fermentor culture were investigated. About 40% (w/w) of the tubers easily lost more than 40% (w/w) of their weight during the first week after being removed from the jar fermentor and stored under room conditions. Sprouting of such easily wilting tubers was clearly delayed whereas other tubers sprouted within 3 months under the same conditions. When the tubers were transplanted and cultivated under field conditions, a yield decrease was observed with the late sprouting tubers. This suggested that the tubers could be selected according to the decrease in their weight after culture.
  • AKITA Motomu; TAKAYAMA Shinsaku
    Plant Biotechnology Japanese Society for Plant Cell and Molecular Biology 10 (3) 177 - 182 0289-5773 1993/12 [Refereed]
     
    Potato (Solanum tuberosum L.) tubers were propagated in a jar fermentor using a 2 step culture method which consisted of the shoot multiplication step (step 1) and the tuber induction and development step (step 2). Shoots which were multiplied in the aerial phase of the jar fermentor were more valuable for the mass propagation of tubers than the shoots cultured under continuously submerged conditions in the liquid medium. The development of tubers was strongly suppressed under the submerged conditions in step 2, whereas the development of tubers was stimulated only at the surface area of the medium, and this suppression might cause significant variation in tuber weight. Tubers contained about 20% (w/w) dry matter, irrespective of size and localization in the jar fermentor.
  • Y KOBAYASHI; M AKITA; K SAKAMOTO; HF LIU; T SHIGEOKA; T KOYANO; M KAWAMURA; T FURUYA
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER VERLAG 40 (2-3) 215 - 218 0175-7598 1993/11 [Refereed]
     
    The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradiation, was scaled up from flasks to a 10-1 glass jar fermenter, a 95-1 stainless steel jar fermenter, and finally a 500-1 pilot-scale jar fermenter. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0-17.2% (w/w) of the dried cells. In one of the operations of the 500-1 jar fermenter, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).
  • Mass propagation of potato tubers using jar fermentor techniques, (共著)
    秋田 求; 高山真策
    Acta Horticulturae 230 55 - 61 1988 [Refereed]
  • AKITA MOTOMU; YOSHIDA TOMIO
    日本土壌肥料学雑誌 Japanese Society of Soil Science and Plant Nutrition 58 (4) 440 - 443 0029-0610 1987/08 [Refereed]
     
    マセチレン阻害法を用いて, 温室ポット栽培の水稲および陸稲根圏における硝化-脱窒活性をin situ 条件下で測定した. その結果, 水稲根圏において, 生育初期には硝化-脱窒活性の促進が認められ, 出穂期にはさらに活性が高まるものの, 完熟期には消失することが明らかとなった. また, 根圏の硝化-脱窒活性は, 地上部における光合成作用の影響を著しく受けることが明らかとなった. これに対して, 非根圏では, 比較的初期に脱窒活性が認められたが, 以降消失した. また, 得られた活性値も小さかった. 一方, 畑条件下の根圏では, 硝化-脱窒活性が認められなかったが, 土壌水分が最大容水量条件では脱窒活性が認められたことから, 本実験条件下では, 土壌水分が, 脱窒作用の制限要因となっていたことが推定された.
  • 国府田悦男; 荒川健司; 秋田求; 芦田直子; 飯島雅; 北川貴子; 白鳥康彦; 幸重雅也; 前田修
    筑波の環境研究 筑波大学 8 86 - 90 0912-1811 1984 [Refereed]

Books etc

  • 植物バイオテクノロジー, 遺伝子操作
    秋田 求; 高山真策 (Joint work)幸書房 2009/05 
    植物の遺伝子操作について、その原理や応用、および、研究手法を紹介した。
  • Practical aspects of bioreactor application in mass propagation （共著）
    Liquid Culture Systems for in vitro Plant Propagation, Springer Verlag 2005
  • Bioengineering aspects of bioreactor application in plant propagation（共著）
    Plant Tissue Culture Engineering. （Focus on Biotechnology, Vol. 6）, Springer Verlag 2005
  • Practical aspects of bioreactor application in mass propagation (jointly worked)
    Liquid Culture Systems for in vitro Plant Propagation, Springer Verlag 2005
  • Bioengineering aspects of bioreactor application in plant propagation (jointly worked)
    Plant Tissue Culture Engineering. （Focus on Biotechnology, Vol. 6）, Springer Verlag 2005
  • 遺伝子発現解析による環境ストレスの検出 「新農業環境工学-21世紀のパースペクティブ」（日本生物環境調節学会編）
    養賢堂 2004
  • マイクロプロパゲーション「新農業情報工学-21世紀のパースペクティブ」（農業情報学会編）
    養賢堂 2004
  • Development of culture techniques for mass propagation of Colocasia esculenta （共著）
    Potential of root crops for food and industrial resources, International Society for Tropical Root Crops 2002
  • 培養液の組成を測る 「ファイテクHow to みる・きく・はかる −植物環境計測−」（ファイトテクノロジー研究会著）
    養賢堂 2002
  • Bioreactor culture of plant organs
    The Encyclopedia of Cell Technology, Wiley 2000
  • Bioreactor culture of plant organs
    The Encyclopedia of Cell Technology 2000
  • 大型培養槽を用いた植物器官の大量培養
    植物工場技術の新しい展開(SHITA Report NO.7) 1994
  • マイクロチューバー大量培養法
    「最新バイオテクノロジー全書2・野菜の組織,細胞培養と増殖」,農業図書 1990
  • 食用イモ類
    「植物工学要覧」R&Dプランニング(刊) 1989

Conference Activities & Talks

  • Release condition and function of peroxidase (Prx34) in Physcomitrium patens  [Not invited]
    Yuki NAKA; Kenji ITO; Yui HAYASHI; Motomu AKITA
    IAPB 2023, The 15th International Association For Plant Biotechnology Congress  2023/08
  • Highly Active, PH- And Heat-Tolerate Peroxidase (Prx34) Derived From Moss, Physcomitrium Patens, Is Produced In Soluble Form In Escherichia Coli  [Not invited]
    Kenji Ito; Yuki Naka; Kotaro Matsumoto; Motomu Akita
    IAPB2023, The 15th International Association For Plant Biotechnology Congress  2023/08
  • Studies on releasing mechanism of P;from Physcomitrium patens
    Yuki NAKA; Kenji ITO; Yui HAYASHI; Motomu AKITA
    日本農芸化学会2023年度大会  2023/03
  • Recombinant peroxidase Prx34 from Physcomitrium patens strongly responds to chemiluminescence reagents  [Not invited]
    Kenji ITO; Motomu Akita
    日本農芸化学会2023年度大会  2023/03
  • Heterogeneous production of a chitosan responsive peroxidase derived from Physcomitrium patens and its characterization  [Not invited]
    伊藤 健司; 中 雄輝; 秋田 求
    第95回日本生化学会  2022/11
  • Production of recombinant peroxidase derived from Physcomitrella patens in a secretion system of Brevibacillus choshinensis  [Not invited]
    中 雄輝; 伊藤 健司; 秋田 求
    第95回日本生化学会  2022/11
  • ヒメツリガネゴケ由来のペルオキシダーゼ（Prx34）の大腸菌による生産と特徴づけ  [Not invited]
    伊藤 健司; 中 雄輝; Jari P. T. Valkonen; 秋田 求
    第39回 日本植物バイオテクノロジー学会大会  2022/09
  • スギバミズゴケ（Sphagnum capilliforium）のカルスを経由する器官分化  [Not invited]
    泉谷 孝,伊藤健司,秋田 求
    日本生物環境工学会2022年福岡大会  2022/09
  • 無菌培養したスギバミズゴケ（Sphagnum capilliforium)に対するFipexideの影響  [Not invited]
    泉谷 孝; 伊藤健司; 秋田 求
    日本蘚苔類学会第51回島根県江津大会  2022/08
  • Secretion signal of peroxidase from moss, Physcomitrium patens, functions in Escherichia coli.  [Not invited]
    Kenji Ito; Motomu Akita
    International Union Microbiological societies 2022  2022/07
  • Secretion signal of peroxidase from moss, Physcomitrium patens, functions in Escherichia coli.  [Not invited]
    Kenji Ito; Motomu Akita
    International Union Microbiological societies 2022  2022/07
  • 異種生産したヒメツリガネゴケ由来のペルオキシダーゼ (Prx34) の特徴づけ  [Not invited]
    伊藤 健司; 中 雄輝; 秋田 求
    日本農芸化学会2022年度大会  2022/03
  • Transformation of Sphagnum capilliforium  [Not invited]
    泉谷 孝; 藤本 未央; 秋田 求
    日本植物バイオテクノロジー学会  2021/09
  • ヒメツリガネゴケ (Physcomitrium patens) 由来のペルオキシダーゼ (Prx34) の異種生産  [Not invited]
    伊藤 健司; 中 雄輝; Jari P. T. Valkonen; 秋田 求
    第38回 日本植物バイオテクノロジー学会大会  2021/09
  • Analysis of Eleocharis vivipara, a C3/C4 interconvertible sedge plant, using in vitro culture technique  [Not invited]
    Motomu Akita; Takeshi Izumitani; Daijiro Harada
    Japanese-Finnish Seminar2021, Understanding the integrative systems to coordinate paradoxes between enhanced effeciency and stress tolerance of photosynthesis  2021/08
  • Green pigmented cell clusters induced by Fipexide on Sphagnum capilliforium  [Not invited]
    Mio Fujimoto; Shin'ichiro Ako; Masatoshi Sakazaki; Akari Nakahara; Kenji Ito; Motomu Akita
    Bryophytes, lichens, and northern ecosystems in a changing world (BL2021)  2021/07
  • 各種培養に対するガス透過性フィルムの利用  [Not invited]
    栗本聡宣; 三栖尚也; 日比野華蓮; 秋田 求
    日本生物環境工学会  2019/09
  • Characterization of knockout mutant of KNLR1, a NBS-LRR gene of Physcomitrella patens  [Not invited]
    KURIMOTO Satoki; ISHIKURA Tomohiro; Mikko T. Lehtonen,Jari; P. T. Valkonen; AKITA Motomu
    The 37th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2019/09  京都市・京都府立大学
  • コケから調べる植物と病原菌との関係  [Not invited]
    秋田 求
    BOST Science Cafe  2019/06  和歌山県橋本市  近畿大学生物理工学部
  • コケとそれをとりまく微生物との関係～コケが病原性微生物から身を守るしかけ～  [Not invited]
    秋田 求
    平成30年度近畿大学先端技術総合研究所公開シンポジウム  2018/10
  • スナゴケ周辺から単離されたバクテリアとヒメツリガネゴケの相互作用  [Not invited]
    櫻井 陽菜; 日比野 華蓮; 秋田 求
    日本生物環境工学会2018年東京大会  2018/09 
    P2-11
  • ヒメツリガネゴケ由来ペルオキシダーゼ(Prx34)の機能解析  [Not invited]
    鶴川 倫子; 中山 翔平; 秋田 求; Jari; P.T. Valkonen
    日本生物環境工学会2018年東京大会  2018/09 
    P2-09
  • エゾスナゴケ（Racomitrium japonicum）周辺に生息する微生物に関する研究  [Not invited]
    田邊 未夏; 田村 真子; 日比野 華蓮; 秋田求
    日本生物環境工学会2018年東京大会  2018/09 
    P2-08
  • C3/C4光合成転換植物Eleocharis vivipara形質転換条件の検討  [Not invited]
    福永達也; 山口一成; 原田大士朗; 秋田 求
    日本生物環境工学会2018年東京大会  2018/09
  • Production of Physcomitrella patens peroxidase（Prx34）by Pichia pastoris  [Not invited]
    TSURUKAWA Michiko; AKITA Motomu; NAKAYAMA Syohei; VALKONEN; P.T. Jari
    The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2018/08
  • Study for establishment of transformation system of Eleocharis vivipara , a C3/C4 interconvertible sedge plant  [Not invited]
    FUKUNAGA Tatsuya; HARADA Daijiro; YAMAGUCHI Kazunari; AKITA Motomu
    The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2018/08
  • Study for interaction between moss and moss associated bacteria  [Not invited]
    AKITA Motomu
    The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2018/08
  • Pathogenicity of Sclerotium sp. to Physcomitrella patens  [Not invited]
    TANABE Minatsu; AKITA Motomu
    The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2018/08
  • Production of Physcomitrella patens peroxidase （Prx34） by Pichia pastoris  [Not invited]
    TSURUKAWA Michiko; AKITA Motomu; NAKAYAMA Syohei; VALKONEN Jari P.T
    The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2017/08  Sonic City Hole, Omiya, Saitama  The Japanese Society of Plant Cell and Molecular Biology
     
    発表番号 P-107、要旨集 p182
  • Sclerotium delphinii is a harmful fungus to Racomitrium japonicum  [Not invited]
    TANABE Minatsu; MAKO Tamura; AKITA Motomu
    The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2017/08  Sonic City Hole, Omiya, Saitama  The Japanese Society of Plant Cell and Molecular Biology
     
    発表番号 P-049, 要旨集 p153
  • Physcomitrella patens activates defense responses against the pathogen Xanthomonas oryzae  [Not invited]
    SAKURAI Haruna; TAMURA Mako; AKITA Motomu
    The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology  2017/08  Sonic City Hole, Omiya, Saitama  The Japanese Society of Plant Cell and Molecular Biology
     
    発表番号P-047, 要旨集p152
  • C3-C4型光合成転換植物Eleocharis viviparaのC3型/C4型特異的遺伝子の解析  [Not invited]
    秋田 求
    平成２８年度形質転換植物デザイン研究拠点成果報告会  2017/03
  • ヒヨス毛状根のトロパンアルカロイド高生産条件の検討  [Not invited]
    平井俊充; 馬場智也; 秋田求
    日本生物環境工学会2016年金沢大会  2016/09
  • Stimulation of bud induction of Racomitrium japonicum by CO2 enrichment and red light illumination  [Not invited]
    Motomu Akita; Miyuki Tachibana
    Moss2016  2016/09
  • Sclerotium as a harmful pathogen of sunagoke moss (Racomitrium japonicum) and its biological control using Bacillus  [Not invited]
    Mako Tamura; Minatsu Tanabe; Jari P. T. Valkonen; Motomu Akita
    Moss2016  2016/09
  • C4光合成の炭酸固定酵素と脱炭酸酵素のC3植物への導入による代謝系の改変：水ストレス耐性の向上とその基盤のメタボローム解析  [Not invited]
    西村隆秀; 高木祐子; 中山泰宗; 傳寶雄大; 福崎英一郎; 蘆田弘樹; 陀安一郎; 秋田求; 泉井桂
    日本農芸化学会2016年度大会  2016/03
  • 植物に刺激を与えて有用物質の生産性を高める  [Invited]
    秋田 求
    平成27年度スーパー・プロフェッショナル・ハイスクール植物工場講演会  2016/02  福井県小浜市  若狭東高等学校
     
    若狭東高等学校において高校生対象に表記タイトルの講演を行った。
  • C3, C4光合成型相互転換植物Eleocharis viviparaの遺伝子発現解析  [Not invited]
    原田大士朗; 坂本智昭; 倉田哲也; 大和勝幸; 泉井桂; 秋田求
    第５回日本光合成学会年会  2015/05
  • C4光合成のCO2代謝酵素の導入によるC3植物の水利用効率（WUE）および乾燥ストレス耐性の向上：アミノ酸分析とメタボローム解析による性格づけ  [Not invited]
    西村隆秀; 高木祐子; 平野博人; 三輪哲也; 中山泰宗; 傳寶雄大; 福崎英一郎; 秋田求; 泉井桂
    第5回光合成学会年会  2015/05
  • ホルムアルデヒド （FAL）によるストレスに対する植物（アラビドプシスとタバコ）の分子応答とそのシグナル伝達機構の解析  [Not invited]
    西村隆秀; 久保森; 秋田求; 泉井桂; 且原真木; 西村秀希
    第31回資源植物科学シンポジウム・第7回植物ストレス研究シンポジウム  2015/03
  • C3, C4光合成型相互転換植物Eleocharis viviparaの遺伝子発現解析  [Not invited]
    原田大士朗; 坂本智昭; 倉田哲也; 大和勝幸; 泉井桂; 秋田求
    第31回資源植物科学シンポジウム・第7回植物ストレス研究シンポジウム  2015/03
  • Improvement of water use efficiency (WUE) and drought tolerance in tobacco by genetic engineering: Further analyses by metabolomics profiling and δ13C measurement under stress  [Not invited]
    Takahide Nishimura; Hiroki Kitaoka; Yuko Takagi; Yasumune Nakayama; Yudai Denpo; Eiichiro Fukusaki; Ichiro Tayasu; Motomu Akita; Kathura Izui
    第56回日本植物生理学会年会  2015/03
  • HARADA DAIJIRO; SAKAMOTO TOMOAKI; KURATA TETSUYA; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物学会大会研究発表記録  2014/09
  • NISHIMURA TAKAHIDE; HAYASHI KOKI; AKEDO ERISHU; TAKAGI YUKO; NAKAGAWA TSUYOSHI; TANIGUCHI MITSUTAKA; MATSUKURA CHIAKI; EZURA HIROSHI; TAYASU ICHIRO; AKITA MOTOMU; IZUI KATSURA
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2014/07
  • IZUI KATSURA; NISHIMURA TAKAHIDE; TAKAGI YUKO; AKEDO ERISHU; AKITA MOTOMU; ASHIDA HIROKI; YOKOTA AKIHO; TAYASU ICHIRO; NAKAYAMA YASUMUNE; DEMPO YUDAI; FUKUSAKI EIICHIRO; HIRANO HIROTO; MIWA TETSUYA
    日本植物生理学会年会要旨集  2014/03
  • HARADA DAISHIRO; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物生理学会年会要旨集  2014/03
  • Findings of plant disease resistance gene (R-gene) homologues in moss (Physcomitrella patens)  [Invited]
    AKITA Motomu
    MOLECULAR BIOLOGY AND BIOTIC INTERACTIONS OF MOSSES  2013/10
  • TAKAYAMA SHINSAKU; MERA NOBUAKI; AKITA MOTOMU
    日本生物環境工学会大会講演要旨  2013/09
  • TAKAYAMA SHINSAKU; UEMATSU MASAAKI; KAWAMOTO HIROKI; FUJITA YU; AKITA MOTOMU
    日本生物環境工学会大会講演要旨  2013/09
  • TAKAYAMA SHINSAKU; MERA NOBUAKI; AKITA MOTOMU
    日本生物環境工学会大会講演要旨  2013/09
  • HARADA DAISHIRO; SAKAMOTO TOMOAKI; KURATA TETSUYA; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2013/09
  • Comparision of transcriptome between C3/C4 types of photosynthesis on Eleocharis vivipara by the next-generation sequencer  [Not invited]
    Daijiro Harada; Tomoaki Sakamoto; Tetsuya Kurata; Katsuyuki Yamato; Katsura Izui; Motomu Akita
    The 16th International Congress on Photosynthesis  2013/08
  • 遺伝情報の利用による植物の機能改変  [Invited]
    秋田 求
    日本生物環境工学会西日本支部シンポジウム  2013/07
  • Physcomitrella patens has OsXa21 type membrane receptor homologs  [Not invited]
    Yusuke Tanigaki; Kenji Ito; Mikko T Lehtonen; Akiko Kosaka; Katsuyuki Yamato; Jari; P. T. Valkonen; Motomu Akita
    The 16th Annual Moss International Conference  2013/06
  • Characterization of the chitosan-responsive peroxidase Prx34 of Physcomitrella patens  [Not invited]
    Kenji Ito; Yusuke Tanigaki; Mikko T. Lehtonen; Jari P. T. Valkonen; Motomu Akita
    The 16th Annual Moss International Conferenc  2013/06
  • Isolation of antagonistic bacterium against fungi from Sunagoke moss (Racomitrium japonicum)  [Not invited]
    Yoshihiro Morita; Yusuke Tanigaki; Kenji Ito; Wataru Kobori; Akio Tani; Mikko T Lehtonen; Eeva Marttinen, Jari; P.T. Valkonen; Ikko Suzukawa; Haruhiko Murase; Motomu Akita
    The 16th Annual Moss International Conference  2013/06
  • Application of Gas Permeable Film for Cultivation of Aquatic Plant  [Not invited]
    Daijiro Harada; Masato; Hamanaka; Ryoichi Tsuga; Motomu Akita
    2013 IFAC Bio-Robotics Conference  2013/03
  • HARADA DAISHIRO; HIRATA ITSUKI; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物生理学会年会要旨集  2013/03
  • IZUI KATSURA; AKEDO ERISHU; ASHIDA HIROKI; HASHIZUME KEISUKE; YOKOTA AKIHO; AKITA MOTOMU; TAYASU ICHIRO
    日本植物生理学会年会要旨集  2013/03
  • HAYASHI YOSHITAKA; AKEDO ERISHU; HASHIZUME KEISUKE; TAKAGI YUKO; NAKAGAWA TSUYOSHI; TANIGUCHI MITSUTAKA; MATSUKURA CHIAKI; EZURA HIROSHI; AKITA MOTOMU; IZUI KATSURA
    日本植物生理学会年会要旨集  2013/03
  • Transcriptome analysis of C3-C4 photosynthesis interconversion plant Eleocharis vivipara using the next-generation sequencers  [Not invited]
    Daijiro Harada; Itsuki Hirata; Akane Sato; Katsuyuki Yamato; Katsura Izui; Motomu Akita
    The 10th International Congress on Plant Molecular Biology  2012/10
  • AKEDO ERIKA; HIRATA TOMOKO; KAMII KAZUYUKI; TAKAGI YUKO; MIZUNO TAKAFUMI; KOBAYASHI MAKOTO; KOIKE TAKAYOSHI; YAMATO KATSUYUKI; AKITA MOTOMU; IZUI KATSURA
    日本植物学会大会研究発表記録  2012/09
  • 葉緑体内にホスホエノールピルビン酸カルボキシキナーゼを高発現させた Atropa belladonna の特性  [Not invited]
    御前省吾; 川口佳美; 多田朋弘; 泉井桂; 秋田 求
    日本生物環境工学会大会講演要旨  2012/09
  • MISAKI SHOGO; KAWAGUCHI YOSHIMI; TADA TOMOHIRO; IZUI KATSURA; AKITA MOTOMU
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2012/08
  • IZUI KATSURA; AKEDO ERISHU; TAKAGI YUKO; HASHIZUME KEISUKE; HAYASHI YOSHITAKA; NAGAMATSU HIROAKI; NAKAGAWA TSUYOSHI; TANIGUCHI MITSUTAKA; MATSUKURA CHIAKI; EZURA HIROSHI; AKITA MOTOMU
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2012/08
  • KUBO SHIGERU; AKEDO ERISHU; NAKAGAWA TSUYOSHI; ISHIHARA TAKESHI; KONISHI MINEKO; YANAGISAWA SHUICHI; KASAOKA MASAMI; MAKITA YUKO; TOYODA TETSURO; YURIMOTO HIROYA; SAKAI YASUYOSHI; AKITA MOTOMU; IZUI KATSURA
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2012/08
  • HARADA DAISHIRO; HIRATA ITSUKI; SATO AKANE; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2012/08
  • HARADA DAISHIRO; MIZOBATA NATSUKI; YOKOYAMA KANAKO; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物生理学会年会要旨集  2012/03
  • IZUI KATSURA; ASHIDA HIROKI; HASHIZUME KEISUKE; ARIKAWA YOSHIHIRO; HAMAGUCHI YUKO; YOKOMASA TAKEYOSHI; YOKOTA AKIHO; AKITA MOTOMU
    日本植物生理学会年会要旨集  2012/03
  • ITO KENJI; TANIGAKI YUSUKE; LEHTONEN MIKKO; VALKONEN JARI; AKITA MOTOMU
    日本植物生理学会年会要旨集  2012/03
  • KUBO SHIGERU; SAKAKIBARA HITOSHI; KURUSU TAKAMITSU; KUCHITSU KAZUYUKI; YURIMOTO HIROYA; SAKAI YASUYOSHI; AKITA MOTOMU; YAMATO KATSUYUKI; IZUI KATSURA
    日本植物生理学会年会要旨集  2012/03
  • HARADA DAISHIRO; MIZOBATA NATSUKI; YOKOYAMA KANAKO; YOSHIMURA KAZUE; YAMATO KATSUYUKI; IZUI KATSURA; AKITA MOTOMU
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2011/09
  • SUZUKI SHIORI; AKEDO ERISHU; NAKAGAWA TSUYOSHI; SAKAKIBARA HITOSHI; YURIMOTO HIROYA; SAKAI YASUYOSHI; YAMATO KATSUYUKI; AKITA MOTOMU; IZUI KATSURA
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2011/09
  • TANIGAKI YUSUKE; ITO KENJI; KOSAKA AKIKO; LEHTONEN MIKKO; THELANDER MATTIAS; YAMATO KATSUYUKI; AKITA MOTOMU; VALKONEN JARI P. T
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2011/09
  • TANIGAKI YUSUKE; KOSAKA AKIKO; ITO KENJI; LEHTONEN MIKKO; THELANDER MATTIAS; KITSUKAWA MAI; AKITA MOTOMU; VALKONEN JARI
    日本植物生理学会年会要旨集  2011/03
  • SUZUKI SHIORI; AKEDO ERISHU; NAKAGAWA TSUYOSHI; SAKAKIBARA HITOSHI; YURIMOTO HIROYA; SAKAI YASUYOSHI; YAMATO KATSUYUKI; AKITA MOTOMU; IZUI KATSURA
    日本農芸化学会大会講演要旨集  2011/03
  • KUBO SHIGERU; CHIN REIBAI; SAKAKIBARA HITOSHI; YURIMOTO HIROYA; KATO NOBUO; SAKAI YASUYOSHI; AKITA MOTOMU; IZUI KATSURA
    日本農芸化学会大会講演要旨集  2011/03
  • AKITA MOTOMU; KOBORI KO; TANIGAKI YUSUKE; UDO HIROFUMI; MORITA YOSHIHIRO; TANI AKIO; SUZUKAWA KAZUYUKI; LEHTONEN MIKKO; VALKONEN JARI; MURASE HARUHIKO
    日本農芸化学会大会講演要旨集  2011/03
  • HARADA DAIJIRO; KONDO CHIE; YONEHARA RYO; YAMATO KATSUYUKI; AKITA MOTOMU; IZUI KATSURA
    生化学  2011
  • AKEDO ERIKA; SUZUKI SHIORI; FUKUI TAKATO; NAKAGAWA TSUYOSHI; SAKAKIBARA HITOSHI; YURIMOTO HIROYA; SAKAI YASUYOSHI; YAMATO KATSUYUKI; AKITA MOTOMU; IZUI KATSURA
    生化学  2011
  • Characterization of a plant disease resistance gene homolog (PpC24) of Physcomitrella patens  [Not invited]
    秋田 求; Mikko T; Lehtonen Jari; P. T. Valkonen; Mattias Thelander
    MOSS 2010, The 13th annual international conference  2010/07  札幌  MOSS 2010, The 13th annual international conference
     
    ヒメツリガネゴケ（Physcomitrella patens）には、植物病害抵抗性遺伝子のホモログ（PpC24）が見られる。これまで明らかでなかった部分配列を明らかにした。また、そのノックアウト株の性質を明らかにした。
  • Transcriptome Analysis of Formaldehyde Response in Wild-Type and Formaldehyde-Tolerant Transformant of Arabidopsis  [Not invited]
    秋田 求; 泉井 桂; Biotechnology Research Center; Kunming University of Science; Technology, China; 理研・PSC; 東京工業大; 院; 生命理工学; 京都大・院; 農; 京都大・院; 農; 京都大; 院; 農
    International Conference on Arabidopsis Research 2010  2010/06  横浜  International Conference on Arabidopsis Research 2010
     
    HPSとPHIを導入することによってホルムアルデヒド耐性を付与した遺伝子組換えシロイヌナズナと野生株をホルムアルデヒドに暴露した。暴露実験には、この目的に特注したシステムを用いた。暴露後の植物のトランスクリプトームを解析した。結果、ホルムアルデヒド暴露時にみられる特徴的な遺伝子発現レベルの変化を観察でき、そのいくつかは、形質転換株で応答性が低かった。（英文）
  • コケに秘められた可能性 ～緑化資材から遺伝子資源まで～  [Not invited]
    秋田 求
    生物理工学部公開講座  2009/10
  • 分子レベルから植物の応答を調べる（コケの病害応答を例にして）  [Not invited]
    秋田 求
    日本生物環境工学会2008年大会・オーガナイズドセッション  2009/09
  • KOBORI KO; SEKI DAISUKE; AKITA MOTOMU; MURASE HARUHIKO
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2009/07  藤沢  日本植物細胞分子生物学会
     
    エゾスナゴケ（Racomitrium japonicum）の原糸体の無菌培養系を誘導した。その培養特性を報告した。
  • AKITA MOTOMU; KOBORI WATARU; MURASE HARUHIKO; LEHTONEN MIKKO; VALKONEN JARI
    日本農芸化学会大会講演要旨集  2009/03  福岡  日本農芸化学会大会
     
    スナゴケ（Racomitrium japonicum）がカビによる被害を受けることを紹介し、その際に検出されるカビのうちin vitroでコケが被害を受けると同定できたものを報告した。
  • TANIGAKI YUSUKE; MOTOHASHI KEN; HISABORI TOORU; IKEDA KEIKO; MORI HAJIME; IZUI KATSURA; AKITA MOTOMU
    生化学  2008/12  神戸  第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
     
    ポリヘドリンからなる多角体に変異型チオレドキシンを包埋させることにより、チオレドキシンと相互作用するタンパク質を分離しうることを明らかにした。
  • AKITA MOTOMU; LEHTONEN MIKKO; VALKONEN JARI P.T
    日本生物環境工学会大会講演要旨  2008/09  松山  日本生物環境工学会
     
    「未来型植物工場-分子情報利用技術からロボット利用技術まで-」と題したオーガナイズドセッションにおいて、コケの病害応答を例に植物を分子レベルから調べる研究法について概説した。
  • Racomitrium canescens as a material for greening and physiological study  [Not invited]
    ○Motomu Akita; Haruhiko Murase; Akio Tani; Mikko Lehtonen; Jari PT Valkonen
    MOSS 2008, The annual international symposium for moss experimental research 2008  2008/08  Tampere, Finland  MOSS 2008, The annual international symposium for moss experimental research 2008
     
    スナゴケが緑化用の資材として、および、植物とカビ性の病原菌との関係を調べる材料として価値があることを紹介した（英文）。
  • スナゴケの生育を促進する微生物  [Not invited]
    谷 明生; 秋田 求; 金原和秀; 村瀬治比古
    日本生物工学会  2008/08  仙台  日本生物工学会
     
    スナゴケから、その生育を促進するメチロトローフを単離しその効果を確かめた。
  • Physcomitrella responds to chitosan with release of a specific peroxidase  [Not invited]
    M.T. LEHTONEN; M. AKITA; N. KALKKINEN; E. AHOLA-IIVARINEN; G. RONNHOLM; M. THELANDER; J.P.T. VALKONEN
    MOSS 2008, The annual international symposium for moss experimental research 2008  2008/08  Tampere, Finland  MOSS 2008, The annual international symposium for moss experimental research 2008
     
    ヒメツリガネゴケ（Physcomitrella patens）がキトサンに応答してすばやく培地中にペルオキシダーゼを放出すること、および、そのペルオキシダーゼをノックアウトしたときの影響を報告した（英文）
  • TANI AKIO; AKITA MOTOMU; MURASE HARUHIKO; KANEHARA KAZUHIDE
    日本生物工学会大会講演要旨集  2008/07
  • Molecular basis of microbial one-carbon metabolism. Plant-growth promotion by methylobacteria  [Not invited]
    Akio Tani; Motomu Akita; Kosuke Kawaguchi; Hiroya Yurimoto; Yasuyoshi Sakai; Haruhiko Murase; Kazuhide Kimbara
    Gordon Conference  2008/07  Lewiston, Maine  Gordon Conference
     
    コケ（Racomitrium japonicum）から単離されたメチロバクテリウムが植物の生育を促進することを紹介し、その分子的基盤について考察した（英文）。
  • スナゴケの生育を促進する微生物  [Not invited]
    谷 明生; 秋田 求; 金原和秀; 村瀬治比古
    日本農芸化学会中四国支部  2008/05  岡山市  日本農芸化学会中四国支部
     
    スナゴケ（Racomitrium japonicum）の生育を促進するメチロトローフ細菌を単離し、その効果を確かめた。
  • SAKAGAMI AYA; YAMASAKI YASUCHIKA; YOSHIMURA KAZUE; AKITA MOTOMU; IZUI KATSURA
    日本植物生理学会年会要旨集  2008/03
  • 植物組織培養の素敵な世界  [Not invited]
    秋田 求
    生物理工学部公開講座  2007/10
  • TAKAGI KAZUE; AKITA MOTOMU; MURASE OSAHIKO
    日本生物環境工学会大会講演要旨  2007/06
  • KATO KIYOTAKA; OKADA MIZUHO; AKITA MOTOMU; IZUI KATSURA
    日本生物環境工学会大会講演要旨  2007/06
  • KURODA TOSHIAKI; SAITO NAOYA; IKEDA KEIKO; MOTOHASHI TAKESHI; HISAHORI TOORU; IZUI KEI; MORI HAJIME; AKITA MOTOMU
    日本農芸化学会大会講演要旨集  2006/03
  • TAKAYAMA SHINSAKU; IKEMOTO MARIKO; AKITA MOTOMU; OKI HISASHI
    農業環境工学関連学会合同大会講演要旨集(CD-ROM)  2006
  • TAKAYAMA SHINSAKU; IKEMOTO MARIKO; TANI AKIRA; AKITA MOTOMU; OKI HISASHI
    農業環境工学関連学会合同大会講演要旨集(CD-ROM)  2006
  • Elicitor responses in Physcomitrella patens  [Not invited]
    Moss 2006  2006
  • TAKAYAMA SHINSAKU; IKEMOTO MARIKO; AKITA MOTOMU; OKI HISASHI
    農業環境工学関連学会合同大会講演要旨集(CD-ROM)  2006
  • AKITA MOTOMU
    農業環境工学関連7学会合同大会講演要旨集  2005/09
  • 農業工学分野の研究者・技術者のための先端バイオテクノロジー講座「植物の生命情報工学」第3回 植物の遺伝子操作 講演資料集  [Not invited]
    秋田 求
    日本生物環境調節学会研究事業委員会、日本生物環境調節学会近畿支部  2005/09 
    「レタスの種子感染による遺伝子組換え法」について報告した
  • Usefulness of in vitro culture of moss  [Invited]
    秋田 求
    第35回大阪府立大学先端科学研究所生物資源開発センターセミナー  2004/11  大阪  第35回大阪府立大学先端科学研究所生物資源開発センターセミナー
     
    コケの無菌培養系を利用した研究の実例を紹介し、その可能性を明らかにした。
  • KOMAE RUMI; AKITA MOTOMU; OTA YOSHIMOTO
    日本農芸化学会大会講演要旨集  2004/03
  • Mass propagation of cherry (Cerasus yedoensis Matum.) through shoot primordia (jointly worked)  [Not invited]
    5th In Vitro Culture and Horticultural Breeding - Biotechnology, as Theory and Practice in Horticulture  2004
  • AKITA MOTOMU
    日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨  2003/09
  • Moss as a model system for studies on plant-pathogen interactions (jointly worked)  [Not invited]
    Ⅺ International Conference on Molecular Plant-microbe Interactions  2003
  • 農業工学分野の研究者・技術者のための先端バイオテクノロジー講座「植物の生命情報工学」第1回講演資料集  [Not invited]
    日本生物環境調節学会研究事業委員会、日本生物環境調節学会近畿支部  2003 
    「植物のDNAやRNAを扱う意義と基本的方法」というタイトルで研究の概要を解説した。
  • WATANABE TAKASHI; AKITA MOTOMU; OTA YOSHIMOTO
    日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨  2002/08
  • AKITA MOTOMU; OTA YOSHIMOTO
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  2002/07
  • KOMAE RUMI; AKITA MOTOMU; OTA HARUCHIKA; VALKONEN J P T
    日本農芸化学会大会講演要旨集  2002/03
  • HOSHINO MAKI; ICHIHOUSHI MOTOYA; AKITA MOTOMU; OTA HARUCHIKA; IKEDA KEIKO; MORI HAJIME
    日本農芸化学会大会講演要旨集  2002/03
  • A novel gene family in moss(Physcomitrella patens)shares high sequence homology with TIR-NBS class of plant disease resistance genes(共著)  [Not invited]
    Moss 2001 International Meeting on Moss Biology  2001
  • Development of culture techniques for mass propagation of corms of Colocasia esculenta  [Not invited]
    AKITA Motomu; OHTA Yoshimoto
    Twelfth symposium of the International society for tropical root crops  2000/09
  • Bioreactorを用いた植物器官大量培養技術  [Invited]
    秋田 求
    日本植物工場学会関西支部平成10年度講演会  1998/10
  • AKITA MOTOMU; OTA YOSHIMOTO
    日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨  1998/07
  • 組み換え体サトイモの育成:I.サトイモ品種石川早生の黄化茎におけるカルス誘導条件(共著)  [Not invited]
    育種学雑誌(別冊)  1998
  • AKITA MOTOMU; OTA YOSHIMOTO; TAKAI TAKASHIGE
    日本植物工場学会大会学術講演要旨集  1998
  • AKITA MOTOMU; OGURA KAZUKI; NAGAO TOMOAKI; OTA YOSHIMOTO
    日本植物細胞分子生物学会大会・シンポジウム講演要旨集  1997/07
  • AKITA MOTOMU; HATA MITSUNORI; KURIHARA MASAHIKO; OTA YOSHIMOTO
    日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨  1997/06
  • AKITA MOTOMU; OTA YOSHIMOTO
    植物細胞分子生物シンポジウム講演要旨集  1996/07
  • Bioreactor advances for the large-scale production of propagules.(共著)  [Not invited]
    COST 822,Workshop on somatic Embryogenesis,Artificial Seeds and Bioreactors  1996
  • Development of a system for mass propagation of Colocasia esculenta in large scale without forced aeration  [Not invited]
    International symposium on Plant Production in Closed Ecosystems -Automation,Culture,and Environment  1996
  • AKITA MOTOMU
    SHITA Rep  1994/01
  • 秋田求; 小泉蓉子; 川村道生
    日本植物工場学会大会学術講演要旨集  1993
  • Large scale culture of plant organs for production of the secondary metabolites（共著）  [Not invited]
    Phytochemical Potential of Tropical Plants, Second Joint Meeting of the Phytochemical Societies of Europe & North America  1992
  • 大型培養槽を用いた植物器官培養技術に関する研究（共著）  [Not invited]
    日本植物工場学会平成４年度大会  1992
  • 秋田求; 高山真策; 田中秀夫
    植物組織培養学会大会,シンポジウム講演要旨集  1991/07
  • 植物の器官大量培養用培養槽に関する研究（共著）  [Not invited]
    第11回日本植物組織培養学会大会  1989
  • Development of bioreactor specialized for differentiated plant tissue and organ cultures and its application for secondary metabolite production（共著）  [Not invited]
    International Symposium on Application of Biotechnological Methods and Recent Accomplishments of Economic Value in Asia  1989
  • ジャーファーメンターによるサトイモの大量増殖に関する研究（共著）  [Not invited]
    第11回日本植物組織培養学会大会  1989
  • ジャーファーメンターによるジャガイモ塊茎の大量培養に関する研究（共著）  [Not invited]
    第11回日本植物組織培養学会大会  1989
  • 植物器官大量培養装置の開発（共著）  [Not invited]
    1989年度日本農芸化学会大会  1989
  • 植物器官大量培養装置の開発に関する研究（共著）  [Not invited]
    1989年度精密工学会春季大会  1989
  • 組織培養法によるジャガイモ塊茎形成に関する研究 第５報－培養由来の塊茎の性状について－（共著）  [Not invited]
    園芸学会平成１年度春季大会  1989
  • ジャーファーメンターによるサトイモ科植物の大量増殖に関する研究 第２報－サトイモ球茎の大量増殖－（共著）  [Not invited]
    園芸学会平成１年度春季大会  1989
  • 組織培養法によるジャガイモ塊茎の大量増殖に関する研究  [Invited]
    秋田 求
    植物の組織培養技術部会第33回定例会  1988/11
  • 組織培養法によるジャガイモ塊茎形成に関する研究 第４報－塊茎形成におよぼすホルモン条件の影響－  [Not invited]
    園芸学会昭和63年秋季大会  1988
  • AKITA MOTOMU; TAKAYAMA SHINSAKU
    日本植物組織培養学会植物組織培養シンポジウム講演要旨集  1988
  • Mass propagation of potato tubers using jar fermentor techniques (jointly worked)  [Not invited]
    Symosium on High Technology in Protected Cultivation, Internationa Society for Horiticultural Science  1988
  • 組織培養法によるジャガイモ塊茎形成に関する研究 第３報－培養方法による塊茎形成部位の変化－（共著）  [Not invited]
    園芸学会昭和63年春季大会  1988
  • ジャーファーメンターを用いた植物の大量増殖技術  [Invited]
    秋田 求
    日本農業気象学会若手研究者の会winter seminar  1987/11
  • 組織培養法によるジャガイモ塊茎形成に関する研究 第２報－液体培養法を用いた塊茎の形成－（共著）  [Not invited]
    園芸学会昭和62年秋季大会  1987
  • 組織培養法によるジャガイモ塊茎形成に関する研究 第１報－無菌培養系の確立と塊茎形成に関する諸条件の検討－（共著）  [Not invited]
    園芸学会昭和62年秋季大会  1987
  • 液体培養法によるグラジオラスの繁殖に関する研究（共著）  [Not invited]
    第10回日本植物組織培養学会大会  1987
  • 植物根圏における脱窒活性のin situ測定  [Not invited]
    秋田求; 吉田冨男
    昭和61年度日本土壌肥料学会大会  1986/04
  • Mass propagation of plants by fermenter culture techniques (jointly worked)  [Not invited]
    VI International Congress of Plant Cell, Tissue and Organ Culture  1986

Works

  • コケの分子生物学（特に他微生物との相互作用）に関する共同研究
    1999
  • Joint Research on molecular biology of moss
    1999

MISC

Industrial Property Rights

Awards & Honors

  • 2007 日本生物環境工学会西日本支部支部功績賞
     JPN
  • 2006 日本植物工場学会学術会長賞
     JPN
  • 1993 日本植物工場学会奨励賞
     JPN

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2010 -2012 
    Author : Katsura IZUI; Motomu AKITA
     
    Previously we succeeded in conferring an ability of absorbing and assimilating formaldehyde to plants by genetic engineering. In order to put this invention to practical use, (1) the improvements were made on the gene constructs to be expressed, (2) the molecular mechanism of toxicity of formaldehyde was partly elucidated, and (3) the applicability of this invention to a horticultural plant and a monocot plant, rice, was also demonstrated. As a step toward development of plants with an ability to assimilate methane, further introduction of the gene for methanol oxidase was also initiated.
  • 緑化用培養スナゴケの大規模栽培と利用技術の実用化研究
    （独）農業・食品産業技術総合研究機構：民間実用化研究促進事業
    Date (from‐to) : 2008/10 -2011/03 
    Author : 本庄 浩二
     
    コケ緑化の事業化を目指す諸課題のうち、種苗の無菌培養法、肥料開発、病害の発生可能性について分担した。
  • Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(B))
    Date (from‐to) : 2007 -2009 
    Author : Katsura IZUI; Motomu AKITA
     
    The two enzymes which are involved in the formaldehyde (HCHO) fixation in a methanol utilizing bacterium were expressed both in Arabidopsis (At) and tobacco. The obtained transformants showed an augmented capacity of absorbing HCHO and an enhanced tolerance to HCHO. Genome-wide analysis of the genes whose expression were affected in response to HCHO, gave clues to the mechanisms of toxicity of and tolerance to HCHO. The plasmids for the efficient expression of these enzyme genes in plants were constructed, and trials were made to obtain transgenic ornamental plants which will be useful for phytoremediation of HCHO-polluted indoor air.
  • 屋上･壁面緑化用多機能性バイオ苔のLED 活用高効率生産技術
    近畿経済産業局：地域新生コンソーシアム研究開発事業
    Date (from‐to) : 2007/07 -2008/03 
    Author : 川本 八郎
     
    緑化用のスナゴケの無菌増殖系の誘導とその効率的な増殖条件、分化条件の検討を行った。また、スナゴケの液体増殖に適当な肥料の開発も試みた。
  • 文部科学省：科学研究費補助金(萌芽研究)
    Date (from‐to) : 2003 -2005 
    Author : 太田 喜元; 秋田 求; 秋田 求
     
    カイコ細胞質多角体病ウイルスの多角体形成に関与するポリヘドリン遺伝子と、VP3にマウス由来のFosBを接続したキメラ遺伝子を含むバイナリーベクターを作成し、アグロバクテリウムを介してレタスやタバコBY-2細胞などを形質転換した。結果、ウエスタン法による検出ができたものの含量が少なく、生産物のサイズが小さくなっていたなど、さらに検討を要する問題が多いことが明らかになった。そこで、当初の予定を変更し、相互作用するタンパク質の分離が多角体を用いて可能かどうかをカイコ培養細胞を使って調製した多角体を使って確かめることにした。研究では、ホウレンソウ由来のチオレドキシン(Trx-m)を提示させた多角体を作成し、これを用いてTrx-mと相互作用するタンパク質群の分離を試みた。Trx-mとして、S-S結合の形成に関与するシステイン残基の一つをセリンに置換したTrx-m_を用いた。これをVP3遺伝子に連結し、カイコ培養細胞を使用して組換え多角体を生産した。この多角体を用いて、ホウレンソウ葉緑体の粗抽出液からTrx-mと相互作用するタンパク質のいくつか(Rubisco Activaseなど)を分離することに成功した。具体的には、多角体をDTTで前処理して洗浄し、次にタンパク質と反応させ、最後にDTT処理して多角体から溶出させることで相互作用するタンパク質の分離を行うことができた。この洗浄方法や特異性の高さ、繰り返し利用性のなどについては今後も検討を続け、洗練された方法とする価値があると考えられた。
  • 植物の有用代謝物生産方法に関する研究
    その他の研究制度
    Date (from‐to) : 1998
  • Study on tissue culture techniques for production of plant secondary metabolites.
    The Other Research Programs
    Date (from‐to) : 1998
  • Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(A))
    Date (from‐to) : 1995 -1997 
    Author : Yoshimoto OHTA; Motomu AKITA
     
    We attempted to develop a system for commercial production of storage organs of plants using a simplified bioreactor.Taro (Colocasia esculanta) and potato (Solanum tuberosum) were used as model plants in this study. Taro could be grown under a statical and completely submerged condition in the liquid medium. This indicates that taro can be cultured under the condition that the oxygen is supplied only by diffusion from the medium surface and forced aeration is not always necessary. Because forced aeration is thought to be one of the most complicated operations for large scale culture, elimination of the forced aeration is great advantage in simplifying the system. We designed a rotary drum type bioreactor in which the explants were cultured without forced aeration. Oxygen was supplied passively through a silicone spongy plug. Plants were entangled and fixed on a matrix inside of the bioreactor. By filling the bioreactor with a suitable volume of the liquid medium, plants were intermittently immersed into the medium by rotation. Corms of taro were successfully propagated in this system. Potato microtubers could also be mass propagated efficiently in this system.Cultured corms of taro were considered not to be mature because the content of storage protein and starch was significantly low. Taro corms could not be stored long term after finishing culture and their acclimatization just after the culture was easy, whereas root formation was not vigorous. The root formation was stimulated by culturing the corms in pure water or antioxidant solutions at the latest week of the culture and this increased the ratio of survived plants during cultivation was promoted.Our results indicate that the simplified culture system is applicable for mass propagation of corms of taro and microtubers of potato. Possible system for commercial production of the storage organs was discussed.
  • 文部科学省：科学研究費補助金(奨励研究(A))
    Date (from‐to) : 1994 -1994 
    Author : 秋田 求
     
    1.器官培養用の培養装置の開発実験材料として、ベラドンナの不定根を用いた。ドラム型培養装置、および、通気撹拌型培養槽を用いて、ベラドンナ不定根培養系に適した装置の試作を試みた。まず、気相中に不定根を保持し培地を霧状にして供給する方法を試みたが、安定して培養でき、かつ、培養条件を容易に制御できるような実験用装置の構築は困難であった。種々の検討の結果、ドラム型培養槽内に金網などメッシュ状の内筒を設置し、これに、分化した器官を捕捉させるようにして培養する方法が、本研究目的に最も近い形と考えられた。この方法では、培養中に、金網や金網表面に張り付けた多孔質担体(大孔径のスポンジ等)に自動的に植物器官が付着した。培地量や回転数を変化させれば、培地中への浸漬時間やそのタイミングを制御することが可能である。また、通気撹拌型培養槽の撹拌軸に金網からなるカゴを固定し、これに不定根を付着させ培養する方法について、現在、検討を行っている。2.実験に適した培養系の開発実験に用いた培養系は、きわめて安定に分化することが確かめられている。ここから、全く同じく条件下でも分化せず、かつ液体振とう培養可能な細胞を分離し、増殖させることに成功した。さらに、この脱分化細胞を分離する過程で、一旦は脱分化するものの、全く同じ培地条件で継代すると分化し始める株が確認された。このように、「分化」という現象に関連した生化学的な変化を解析するうえで、有用な培養系が得られた。しかし、培養槽を用いてこれらの系を培養することに関しては、特に最後に述べた培養系の維持が困難であり、詳細な条件検討を進めている。3.環境条件の変化に対する反応さらに、活性酸素に関連する物質の影響について検討する予定であったが、これについては、実施に至らなかった。
  • 植物の二次代謝制御機構に関する研究
    その他の研究制度
    Date (from‐to) : 1988
  • study on control mechanism on plant secondary metabolism.
    The Other Research Programs
    Date (from‐to) : 1988
  • 植物の大量増殖法に関する研究
    その他の研究制度
    Date (from‐to) : 1985
  • Study on mass propagation methods by plant tissue culture techniques.
    The Other Research Programs
    Date (from‐to) : 1985
  • Research on techniques for production of plant metabolites
  • Research on bioreactor techniques for mass propagation of seedlings.

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