MATSUHASHI Tamako
Advanced Technology Research Institute | Lecturer |
Last Updated :2024/09/14
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- 分子遺伝学 Wild Animal Japanese Black Cattle animal reproduction molecular biology animal science
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- 血中エクソソームに内包された牛枝肉成績バイオマーカーマイクロRNAの探索松橋 珠子; 笠原 喜斗; 加藤 亜惟; 茶原 裕佳; 池上 春香; 村瀬 華梨; 白木 琢磨; 松本 和也日本獣医学会学術集会講演要旨集 (公社)日本獣医学会 164回 [HLO - 35] 1347-8621 2021/09
- Fuki Kawaguchi; Miyako Tsuchimura; Kenji Oyama; Tamako Matsuhashi; Shin Maruyama; Hideyuki Mannen; Shinji SasazakiArchives Animal Breeding Copernicus GmbH 63 (1) 9 - 17 2020/01 [Refereed]
Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement. - 松橋 珠子関西畜産学会報 = Kansai journal of animal science 関西畜産学会 (176) 1 - 8 1344-9265 2019/03 [Invited]
- 松橋珠子食肉に関する助成研究調査成果報告書 36 166‐171 2018/11
- 松橋珠子近畿大学先端技術総合研究所紀要 (23) 31‐36 1346-8693 2018/03 [Refereed]
- Discovery of biomarker candidate proteins for predicting beef marbling by serum proteomic analysis in Japanese Black steersHaruka Ikegami; Tamako Matsuhashi; Kouhei Nagai; Kei Miyamoto; Kengo Obayashi; Shin-ichi Sakaguchi; Kazuya MatsumotoKANSAI JOURNAL OF ANIMAL SCIENCE 174 1 - 10 2018/03 [Refereed]
- Takashi Hirano; Tamako Matsuhashi; Kenji Takeda; Hiromi Hara; Naohiko Kobayashi; Kazuo Kita; Yoshikazu Sugimoto; Kei HanzawaANIMAL SCIENCE JOURNAL WILEY-BLACKWELL 87 (9) 1178 - 1181 1344-3941 2016/09 [Refereed]
Isoleucyl-tRNA synthetase (IARS) c.235G>C (p.V79L) is a causative mutation for a recessive disease called IARS disorder in Japanese black cattle. The disease is involved in weak calf syndrome and is characterized by low birth weight, weakness and poor suckling. The gestation period is often slightly extended, implying that intrauterine growth is retarded. In a previous analysis of 2597 artificial insemination (AI) procedures, we suggested that the IARS mutation might contribute toward an increase in the incidence of prenatal death. In this study, we extended this analysis to better clarify the association between the IARS mutation and prenatal death. The IARS genotypes of 92 animals resulting from crosses between carrier (G/C) x G/C were 27 normal (G/G), 55G/C and 10 affected animals (C/C) (expected numbers: 23, 46 and 23, respectively). Compared to the expected numbers, there were significantly fewer affected animals in this population (P<0.05), suggesting that more than half of the affected embryos died prenatally. When the number of AI procedures examined was increased to 11580, the frequency of re-insemination after G/C x G/C insemination was significantly higher at 61-140 days (P<0.001). The findings suggested that the homozygous IARS mutation not only causes calf death, but also embryonic or fetal death. - Takahiro Nishimaki; Takayuki Ibi; Siqintuya; Naohiko Kobayashi; Tamako Matsuhashi; Takayuki Akiyama; Emi Yoshida; Kazumi Imai; Mayu Matsui; Keiichi Uemura; Hisayoshi Eto; Naoto Watanabe; Tatsuo Fujita; Yosuke Saito; Tomohiko Komatsu; Hiroshi Hoshiba; Hideyuki Mannen; Shinji Sasazaki; Tetsuo KuniedaANIMAL SCIENCE JOURNAL WILEY 87 (4) 469 - 476 1344-3941 2016/04 [Refereed]
Marker-assisted selection (MAS) is expected to accelerate the genetic improvement of Japanese Black cattle. However, verification of the effects of the genes for MAS in different subpopulations is required prior to the application of MAS. In this study, we investigated the allelic frequencies and genotypic effects for carcass traits of six genes, which can be used in MAS, in eight local subpopulations. These genes are SCD, FASN and SREBP1, which are associated with the fatty acid composition of meat, and NCAPG, MC1R and F11, which are associated with carcass weight, coat color and blood coagulation abnormality, respectively. The frequencies of desirable alleles of SCD and FASN were relatively high and that of NCAPG was relatively low, and NCAPG was significantly associated with several carcass traits, including carcass weight. The proportions of genotypic variance explained by NCAPG to phenotypic variance were 4.83 for carcass weight. We thus confirmed that NCAPG is a useful marker for selection of carcass traits in these subpopulations. In addition, we found that the desirable alleles of six genes showed no negative effects on carcass traits. Therefore, selection using these genes to improve target traits should not have negative impacts on carcass traits. - 丸山新; 勝田智博; 西和隆; 松橋珠子; 山田英信; 向島幸司肉用牛研究会報 (100) 34‐35 0386-8419 2016/01
- Shinji Sasaki; Takayuki Ibi; Tamako Matsuhashi; Kenji Takeda; Shogo Ikeda; Mayumi Sugimoto; Yoshikazu SugimotoBMC GENETICS BIOMED CENTRAL LTD 16 (1) 123 1471-2156 2015/10 [Refereed]
Background: Female fertility, a fundamental trait required for animal reproduction, has gradually declined in the last 2 decades in Japanese Black cattle. To identify associated genetic variants in Japanese Black cattle, we evaluated female fertility as a metric to describe the average inverse of the number of artificial inseminations required for conception from the first through the fourth parity (ANAI(4)) and conducted a genome-wide association study (GWAS) using 430 animals with extreme ANAI(4) values from 10,399 animals. Results: We found that 2 variants, namely a single-nucleotide polymorphisms (SNP; g.48476925C > T) and a 3-bp indel (g.48476943_48476946insGGC), in the upstream region of the activin receptor IIA gene (ACVR2A) were associated with ANAI(4). ACVR2A transcripts from Japanese Black cattle of the Q haplotype, defined by the SNP and the 3-bp indel, with increased ANAI(4) were 1.29-1.32-fold more abundant than q-derived transcripts. In agreement, reporter assay results revealed that the activity of the ACVR2A promoter was higher in reporter constructs with the Q haplotype than in those with the q haplotype by approximately 1.2 fold. Expression of exogenous ACVR2A induced dose-dependent increases of reporter activity from the follicle-stimulating hormone, beta polypeptide (FSHB) promoter in response to activin A in a pituitary gonadotrophic cell line. The findings suggested that sequence variations in the upstream region of ACVR2A with the Q haplotype increased ACVR2A transcription, which in turn induced FSHB expression. This association was replicated using a sample population size of 1,433 animals; the frequency of the Q haplotype was 0.39, and Q-to-q haplotype substitution resulted in an increase of 0.02 in terms of ANAI(4). Conclusions: This GWAS identified variants in the upstream region of ACVR2A, which were associated with female fertility in Japanese Black cattle. The variants affected the level of ACVR2A mRNA expression, which could lead to an allelic imbalance. This association was replicated with a sample population of 1,433 animals. Thus, the results suggest that the Q haplotype could serve as a useful marker to select Japanese Black cattle with superior female fertility. - Akiko Takasuga; Kunio Sato; Ryouichi Nakamura; Yosuke Saito; Shinji Sasaki; Takehito Tsuji; Akio Suzuki; Hiroshi Kobayashi; Tamako Matsuhashi; Koji Setoguchi; Hiroshi Okabe; Toshitake Ootsubo; Ichiro Tabuchi; Tatsuo Fujita; Naoto Watanabe; Takashi Hirano; Shota Nishimura; Toshio Watanabe; Makio Hayakawa; Yoshikazu Sugimoto; Takatoshi KojimaPLOS GENETICS PUBLIC LIBRARY SCIENCE 11 (8) e1005433 1553-7404 2015/08 [Refereed]
Recessive skeletal dysplasia, characterized by joint- and/or hip bone-enlargement, was mapped within the critical region for a major quantitative trait locus (QTL) influencing carcass weight; previously named CW-3 in Japanese Black cattle. The risk allele was on the same chromosome as the Q allele that increases carcass weight. Phenotypic characterization revealed that the risk allele causes disproportional tall stature and bone size that increases carcass weight in heterozygous individuals but causes disproportionately narrow chest width in homozygotes. A non-synonymous variant of FGD3 was identified as a positional candidate quantitative trait nucleotide (QTN) and the corresponding mutant protein showed reduced activity as a guanine nucleotide exchange factor for Cdc42. FGD3 is expressed in the growth plate cartilage of femurs from bovine and mouse. Thus, loss of FDG3 activity may lead to subsequent loss of Cdc42 function. This would be consistent with the columnar disorganization of proliferating chondrocytes in chondrocyte-specific inactivated Cdc42 mutant mice. This is the first report showing association of FGD3 with skeletal dysplasia. - Kiri Hayakawa; Takayuki Sakamoto; Atsushi Ishii; Keita Yamaji; Yoshinobu Uemoto; Nanae Sasago; Eiji Kobayashi; Naohiko Kobayashi; Tamako Matsuhashi; Shin Maruyama; Hirokazu Matsumoto; Kenji Oyama; Hideyuki Mannen; Shinji SasazakiANIMAL SCIENCE JOURNAL WILEY-BLACKWELL 86 (8) 737 - 746 1344-3941 2015/08 [Refereed]
The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle. - Ikegami H; Nagai K; Matsuhashi T; Kobahashi N; Takemoto A; Yoshihiro T; Inoue E; Higuchi C; Morita K; Uchibori S; Amano T; Taguchi Y; Kato H; Iritani A; Matsumoto KNihon Chikusan Gakkaiho Zootechnical Science Society of Japan 86 (2) 141 - 152 1880-8255 2015/06 [Refereed]
Early assessment of carcass traits and meat quality characteristics during fattening is expected to improve the efficiency of beef cattle production. Yet, there have been few reports on developing an assessment index for feeding condition of Japanese Black cattle. In this study, to identify protein biomarkers for predicting carcass traits and meat quality characteristics of Japanese Black cattle by proteomics, we first compared protein expression profiles of perirenal white adipose tissue between low and high merit steers in five carcass traits (carcass weight, rib eye area, rib thickness, subcutaneous fat thickness, and beef marbling standard [BMS] number). We then investigated the involvement of the lower- or higher-expressed proteins in metabolic pathways and evaluated the expression of vimentin proteins by western blot analysis in individual steers. We identified 45 proteins which were significantly expressed in low or high merit groups of five carcass traits. Coordinate involvement of some identified proteins in glycolysis/gluconeogenesis and citrate cycle pathways was found in high merit groups of carcass weight, subcutaneous fat thickness and BMS number. The expression of vimentin protein, which was significantly expressed in the high BMS number group, was confirmed in individual steers. Taken together, these results suggest the possibility of these identified proteins as protein biomarker candidates for predicting carcass traits and meat quality characteristics during fattening of Japanese Black cattle. - MATSUHASHI TamakoThe Journal of animal genetics Japanese Society of Animal Breeding and Genetics 42 (2) 71 - 77 1345-9961 2014/12 [Invited]
- 渡辺 大作; 谷地村 結未; 武田 祐典; 野村 徹; 富岡 美千子; 平野 貴; 松橋 珠子; 杉本 喜憲産業動物臨床医学雑誌 日本家畜臨床学会 5 (2) 137 - 139 1884-684X 2014/11
- Nagai Kouhei; Ikegami Haruka; Matsuhashi Tamako; Takemoto Atsushi; Minakata Yusuke; Higuchi Chika; Morita Koutaro; Kobayashi Naohiko; Matsumoto KazuyaAbstracts for Annual Meeting of Japanese Proteomics Society Japanese Proteomics Society (Japan Human Proteome Organisation) 2014 98 - 98 2014
- Atsushi Ishii; Keita Yamaji; Yoshinobu Uemoto; Nanae Sasago; Eiji Kobayashi; Naohiko Kobayashi; Tamako Matsuhashi; Shin Maruyama; Hirokazu Matsumoto; Shinji Sasazaki; Hideyuki MannenANIMAL SCIENCE JOURNAL WILEY-BLACKWELL 84 (10) 675 - 682 1344-3941 2013/10 [Refereed]
Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome-wide association study with 50K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40657 SNPs were used in this study. We applied genome-wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl-CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition. - KOBAYASHI Eiji; OKUMURA Naohiko; MINATO Kazuyuki; KUROKI Masahiro; YASUDA Yasuaki; NII Masahiro; MATSUHASHI TamakoThe Japanese Journal of Swine Husbandry Research 日本養豚学会 50 (3) 137 - 141 0913-882X 2013/09 [Refereed]
ミトコンドリアDNA(mtDNA)は,母性遺伝すること,変異率が高いことおよびその変異が蓄積することなどから,集団遺伝学的解析に広く用いられている(CASTROら,1998; BOORE,1999)。ブタにおけるmtDNAの解析から,その塩基配列により大きくヨーロッパ型とアジア型に分類され,ニホンイノシシはリュウキュウイノシシとはやや異なるものの,同じアジア型に分類されている(OKUMURAら,1996;2001; WATANOBEら,1999)。一方,イノシシにおける核ゲノムDNAの分析は,ブタの家畜化起源の研究の中で,幾つかの毛色関連遺伝子等が分析されている(GIUFFRAら,2000; LARSONら,2005;2010)。特に,ニホンイノシシでは,第1番染色体上のGPIP遺伝子(Glucosephosphate isomerase-processed pseudogene)がよく調べられている(ISHIGUROら,2002;2005;2008)。しかしながら,GPIP遺伝子においては欧米品種を分析した報告が多くないことおよびほとんどの変異が塩基配列の決定によることなどから,分析の効率化が必要である。成長ホルモン(Growth hormone,GH)遺伝子は,第12番染色体上に位置し,ブタの成長に関与すると期待される遺伝子であり,大別するとヨーロッパ型,アジア型および共通型の3種に分類されている(小林ら,1999)。そこで本研究では,豚遺伝子の流入状況を調査するための基礎情報として,2000年時点のニホンイノシシについて集団遺伝学的調査を行った。また,mtDNAを簡易に分析するため,ミスマッチプライマーを用いたPCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism)法によるヨーロッパ型またはアジア型の判定法を確立し,合わせて分析した。 - Takahiro Nishimaki; Takayuki Ibi; Yoshihiro Tanabe; Yoshiyuki Miyazaki; Naohiko Kobayashi; Tamako Matsuhashi; Takayuki Akiyama; Emi Yoshida; Kazumi Imai; Mayu Matsui; Keiichi Uemura; Naoto Watanabe; Tatsuo Fujita; Yosuke Saito; Tomohiko Komatsu; Takahisa Yamada; Hideyuki Mannen; Shinji Sasazaki; Tetsuo KuniedaANIMAL SCIENCE JOURNAL WILEY-BLACKWELL 84 (8) 585 - 591 1344-3941 2013/08 [Refereed]
Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise F-ST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies. - Takashi Hirano; Naohiko Kobayashi; Tamako Matsuhashi; Daisaku Watanabe; Toshio Watanabe; Akiko Takasuga; Mayumi Sugimoto; Yoshikazu SugimotoPLoS ONE 8 (5) e64036 1932-6203 2013/05 [Refereed]
We identified an IARS (isoleucyl-tRNA synthetase) c.235G> C (p.Val79Leu) substitution as the causative mutation for neonatal weakness with intrauterine growth retardation (perinatal weak calf syndrome). In Japanese Black cattle, the syndrome was frequently found in calves sired by Bull A. Hence, we employed homozygosity mapping and linkage analysis. In order to identify the perinatal weak calf syndrome locus in a 4.04-Mb region of BTA 8, we analysed a paternal half-sibling family with a BovineSNP50 BeadChip and microsatellites. In this critical region, we performed exome sequencing to identify a causative mutation. Three variants were detected as possible candidates for causative mutations that were predicted to disrupt the protein function, including a G> C (p.Val79Leu) mutation in IARS c.235. The IARS c.235G> C mutation was not a homozygous risk allele in the 36 healthy offspring of Bull A. Moreover, the IARS Val79 residue and its flanking regions were evolutionarily and highly conserved. The IARS mutant (Leu79) had decreased aminoacylation activity. Additionally, the homozygous mutation was not found in any of 1526 healthy cattle. Therefore, we concluded that the IARS c.235G> C mutation was the cause of hereditary perinatal weak calf syndrome. © 2013 Hirano et al. - Shinji Sasaki; Takayuki Ibi; Toshio Watanabe; Tamako Matsuhashi; Shogo Ikeda; Yoshikazu SugimotoBMC GENETICS BIOMED CENTRAL LTD 14 (14) 41 1471-2156 2013/05 [Refereed]
Background: Calving efficiency can be described as the measure of a cow's ability to produce viable offspring within a specific period of time. This trait is crucial in beef cattle because calves are necessary both for the production of beef and for heifer replacements. Recently, the number of calves produced at 4 years of age (NCP4) has been used to evaluate the calving efficiency of Japanese Black cattle. To identify variants associated with calving efficiency in Japanese Black cattle, we conducted a genome-wide association study (GWAS) using 688 animals with extreme NCP4 values selected from 15,225 animals. Results: We identified genetic variants on bovine chromosome 12 (BTA12) that were associated with NCP4. The General Transcription Factor IIF, polypeptide 2 (GTF2F2), located in the 132 kbp-associated region, proved to be in strong linkage disequilibrium. We found 15 associated variants in the promoter and the 3' UTR regions. Consistent with this finding, transcripts of GTF2F2 derived from the haplotype (Q) with the increased number of calves were 1.33-fold more abundant than q-derived transcripts. Furthermore, luciferase assays revealed that the activity of the 3' UTR, a region that includes nine SNPs, was higher in constructs with the Q haplotype than in those with the q haplotype by approximately 1.35-fold. In contrast, the activity of the promoter region did not differ between haplotypes. The association was replicated in an independent sample of 827 animals that were randomly selected from the remainder of the cohort from the same farms used in the GWAS. In the replicated population, the frequency of the Q haplotype is 0.313, and this haplotype accounts for 2.69% of the total phenotypic variance. The effect of the Q to q haplotype substitution on NCP4 was 0.054 calves. These findings suggest that variants in the 3' UTR of GTF2F2 affect the level of GTF2F2 mRNA, which is associated with calving efficiency. Conclusions: This GWAS has identified variants in the 3' UTR of GTF2F2 that were associated with the NCP4 of Japanese Black cattle, and this association was validated in an independent sample. The Q haplotype will be immediately useful in improving the calving efficiency of Japanese Black cattle. - T. Miyasaka; S. -N. Takeshima; M. Jimba; Y. Matsumoto; N. Kobayashi; T. Matsuhashi; H. Sentsui; Y. AidaTISSUE ANTIGENS WILEY 81 (2) 72 - 82 0001-2815 2013/02 [Refereed]
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Bovine leukocyte antigen (BoLA) is strongly involved in the subclinical progression of BLV infections. Recent studies show that the BoLA-DRB3 gene might play a direct role in controlling the number of BLV-infected peripheral B lymphocytes in vivo in Holstein cattle. However, the specific BoLA class II allele and DRB3-DQA1 haplotypes determining the BLV proviral load in Japanese Black cattle are yet to be identified. In this study, we focused on the association of BLV proviral load and polymorphism of BoLA class II in Japanese Black cattle. We genotyped 186 BLV-infected, clinically normal cattle for BoLA-DRB3 and BoLA-DQA1 using a polymerase chain reaction-sequence-based typing method. BoLA-DRB3*0902 and BoLA-DRB3*1101 were associated with a low proviral load (LPVL), and BoLA-DRB3*1601 was associated with a high proviral load (HPVL). Furthermore, BoLA-DQA1*0204 and BoLA-DQA1*10012 were related to LPVL and HPVL, respectively. Furthermore, we confirmed the correlation between the DRB3-DQA1 haplotype and BLV proviral load. Two haplotypes, namely 0902B or C (DRB3*0902-DQA1*0204) and 1101A (DRB3*1101-DQA1*10011), were associated with a low BLV proviral load, whereas one haplotype 1601B (DRB3*1601-DQA1*10012) was associated with a high BLV proviral load. We conclude that resistance is a dominant trait and susceptibility is a recessive trait. Additionally, resistant alleles were common between Japanese Black and Holstein cattle, and susceptible alleles differed. This is the first report to identify an association between the DRB3-DQA1 haplotype and variations in BLV proviral load. - Mayuko Jimba; Shin-nosuke Takeshima; Hironobu Murakami; Junko Kohara; Naohiko Kobayashi; Tamako Matsuhashi; Takashi Ohmori; Tetsuo Nunoya; Yoko AidaBMC VETERINARY RESEARCH BIOMED CENTRAL LTD 8 (8) 167 1746-6148 2012/09 [Refereed]
Background: Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. Recently, we developed a new quantitative real-time polymerase chain reaction (PCR) method using Coordination of Common Motifs (CoCoMo) primers to measure the proviral load of known and novel BLV variants in BLV-infected animals. Indeed, the assay was highly effective in detecting BLV in cattle from a range of international locations. This assay enabled us to demonstrate that proviral load correlates not only with BLV infection capacity as assessed by syncytium formation, but also with BLV disease progression. In this study, we compared the sensitivity of our BLV-CoCoMo-qPCR method for detecting BLV proviruses with the sensitivities of two real-time PCR systems, and also determined the differences of proviral load with serotests. Results: BLV-CoCoMo-qPCR was found to be highly sensitive when compared with the real-time PCR-based TaqMan MGB assay developed by Lew et al. and the commercial TaKaRa cycleave PCR system. The BLV copy number determined by BLV-CoCoMo-qPCR was only partially correlated with the positive rate for anti-BLV antibody as determined by the enzyme-linked immunosorbent assay, passive hemagglutination reaction, or agar gel immunodiffusion. This result indicates that, although serotests are widely used for the diagnosis of BLV infection, it is difficult to detect BLV infection with confidence by using serological tests alone. Two cattle were experimentally infected with BLV. The kinetics of the provirus did not precisely correlate with the change in anti-BLV antibody production. Moreover, both reactions were different in cattle that carried different bovine leukocyte antigen (BoLA)-DRB3 genotypes. Conclusions: Our results suggest that the quantitative measurement of proviral load by BLV-CoCoMo-qPCR is useful tool for evaluating the progression of BLV-induced disease. BLV-CoCoMo-qPCR allows us to monitor the spread of BLV infection in different viewpoint compared with classical serotest. - Ikegami H; Kobayashi N; Matsuhashi T; Takemoto A; Yoshihiro T; Inoue E; Kato R; Kato H; Taguchi Y; Amano T; Morimoto K; Nakagawa M; Iritani A; Matsumoto KNihon Chikusan Gakkaiho 83 (3) 281 - 290 2012/08 [Refereed]
- T. Hirano; T. Matsuhashi; N. Kobayashi; T. Watanabe; Y. SugimotoANIMAL GENETICS WILEY-BLACKWELL 43 (1) 11 - 17 0268-9146 2012/02 [Refereed]
Mutations in the gene encoding fibrillin-1 (FBN1), a component of the extracellular microfibril, cause Marfan syndrome (MFS). Frequent observation of cattle with a normal withers height, but lower body weight than age-matched normal cattle, was recently reported among cattle sired by phenotypically normal Bull A, in Japanese Black cattle. These cattle also showed other characteristic features similar to the clinical phenotype of human MFS, such as a long phalanx proximalis, oval face and crystalline lens cloudiness. We first screened a paternal half-sib family comprising 36 affected and 10 normal offspring of Bull A using the BovineSNP50 BeadChip (illumina). Twenty-two microsatellite markers mapped to a significant region on BTA10 were subsequently genotyped on the family. The bovine Marfan syndrome-like disease (MFSL) was mapped onto BTA10. As FBN1 is located in the significant region, FBN1 was sequenced in Bull A, and three affected and one normal cattle. A G>A mutation at the intron64 splicing accepter site (c.8227-1G>A) was detected in 31 of 36 affected animals (84.7%). The c.8227-1G>A polymorphism was not found in 20 normal offspring of Bull A or in 93 normal cattle unrelated to Bull A. The mutation caused a 1-base shift of the intron64 splicing accepter site to the 3' direction, and a 1-base deletion in processed mRNA. This 1-base deletion creates a premature termination codon, and a 125-amino acid shorter Fibrillin-1 protein is produced from the mutant mRNA. We therefore conclude that the c.8227-1G>A mutation is causative for MFSL. Furthermore, it was suggested that Bull A exhibited germline mosaicism for the mutation, and that the frequency of the mutant sperm was 14.9%. - Ikegami Haruka; Matsuhashi Tamako; Takemoto Atsushi; Nagai Kouhei; Higuchi Tomoka; Morita Koutaro; Kobayashi Naohiko; Matsumoto KazuyaAbstracts for Annual Meeting of Japanese Proteomics Society Japanese Proteomics Society (Japan Human Proteome Organisation) 2012 93 - 93 2012
- Jimba M; Takeshima SN; Matsumoto Y; Kobayashi N; Matsuhashi T; Kohara J; Aida YRetrovirology 8 A21 2011/06 [Refereed]
- Taku Miyasaka; Shin-nosuke Takeshima; Yuki Matsumoto; Naohiko Kobayashi; Tamako Matsuhashi; Yoshiyuki Miyazaki; Yoshihiro Tanabe; Kazuki Ishibashi; Hiroshi Sentsui; Yoko AidaGENE ELSEVIER SCIENCE BV 472 (1-2) 42 - 49 0378-1119 2011/02 [Refereed]
In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for bovine diseases and immunological traits. In this study, we sequenced alleles of the BoLA class II loci, BoLA-DRB3 and BoLA-DQA1, from 650 Japanese cattle from six herds [three herds (507 animals) of Japanese Black cattle and three herds (143 animals) of Holstein cattle] using polymerase chain reaction-sequence-based typing (PCR-SBT) methods. We identified 26 previously reported distinct DRB3 alleles in the two populations: 22 in Japanese Black and 17 in Holstein. The number of DRB3 alleles detected in each herd ranged from 9 to 20. Next, we identified 15 previously reported distinct DQA1 alleles: 13 in Japanese Black and 10 in Holstein. The number of alleles in each herd ranged from 6 to 10. Thus, allelic divergence is significantly greater for DRB3 than for DQA1. A population tree on the basis of the frequencies of the DRB3 and DQA1 alleles showed that, although the genetic distance differed significantly between the two cattle breeds, it was closely related within the three herds of each breed. In addition, Wu-Kabat variability analysis indicated that the DRB3 gene was more polymorphic than the DQA1 gene in both breeds and in all herds, and that the majority of the hypervariable positions within both loci corresponded to pocket-forming residues. The DRB3 and DQA1 heterozygosity for both breeds within each herd were calculated based on the Hardy-Weinberg equilibrium. Only one Japanese Black herd showed a significant difference between the expected and observed heterozygosity at both loci. This is the first report presenting a detailed study of the allelic distribution of BoLA-DRB3 and -DQA1 genes in Japanese Black and Holstein cattle from different farms in Japan. These results may help to develop improved livestock breeding strategies in the future. (C) 2010 Elsevier B.V. All rights reserved. - T. Matsuhashi; S. Maruyama; Y. Uemoto; N. Kobayashi; H. Mannen; T. Abe; S. Sakaguchi; E. KobayashiJOURNAL OF ANIMAL SCIENCE AMER SOC ANIMAL SCIENCE 89 (1) 12 - 22 0021-8812 2011/01 [Refereed]
The quality of fat is an important factor in defining the quality of meat. Fat quality is determined by the composition of fatty acids. Among lipid metabolism-related genes, including fatty acid synthesis genes, several genetic variations have been reported in the bovine fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD), sterol regulatory element-binding protein 1 (SREBP1), and GH genes. In the present study, we evaluated the single and epistatic effects of 5 genetic variations (4 SNP and 1 insertion/deletion) in 4 genes (FASN, SCD, SREBP1, and GH) on the fatty acid composition of the longissimus thoracis muscle and carcass and meat quality traits in 480 commercial Japanese Black cattle. Significant single effects of FASN, SCD, and GH(L127V) polymorphisms on the fatty acid composition of the longissimus thoracis muscle were detected. The A293V polymorphism of SCD had the largest effect on myristic acid (C14:0, P < 0.001), myristoleic acid (C14:1, P < 0.001), stearic acid (C18:0, P < 0.001), oleic acid (C18:1, P < 0.001), and MUFA (P < 0.001). Polymorphisms in the FASN, SCD, and SREBP1 genes showed no effect on any meat yield trait. There were no significant epistatic effects on fatty acid composition among pairs of the 3 genes (FASN, SCD, and SREBP1) involved in fatty acid synthesis. No epistatic interactions (P > 0.1) were detected between FASN and SCD for any carcass trait. When the genotypes of 3 markers (FASN, SCD, and GH(L127V)) were substituted from the lesser effect allele to the greater effect allele, the proportion of C18:1 increased by 4.46%. More than 20% of the genetic variance in the C18: 1 level could be accounted for by these 3 genetic markers. The present results revealed that polymorphisms in 2 fatty acid synthesis genes (FASN and SCD) independently influenced fatty acid composition in the longissimus thoracis muscle. These results suggest that SNP in the FASN and SCD genes are useful markers for the improvement of fatty acid composition in commercial Japanese Black cattle. - 牛白血病ウイルス(BLV)ウイルスロードとMHCクラスII遺伝子の相関性宮坂 卓; 竹嶋 伸之輔; 神馬 繭子; 松本 有生; 小林 直彦; 松橋 珠子; 泉對 博; 間 陽子日本獣医学会学術集会講演要旨集 (公社)日本獣医学会 147回 249 - 249 1347-8621 2009/03
- Tamako Matsuhashi; Tomohiko Akiyama; Fugaku Aoki; Senkiti SakaiANIMAL SCIENCE JOURNAL BLACKWELL PUBLISHING 78 (6) 575 - 586 1344-3941 2007/12 [Refereed]
Gene expression in the implanting blastocyst is altered by stimulation with estrogen secreted from maternal ovaries. In the present study, to understand the mechanism regulating the changes in gene expression, diverse histone modifications in blastocysts were studied using a delayed implantation model, in which embryos were kept in a dormant state in the uterus by maternal ovariectomy and progesterone treatment, and then activated by injection with estrogen. Total transcriptional activity increased markedly in activated embryos, and immunocytochemistry with antibodies recognizing specific histone modifications revealed differential modification of several histones in the trophectoderm (TE) and inner cell mass (ICM) of dormant and activated embryos. High levels of histone H3 lysine 9 (H3K9) dimethylation, which suppresses gene expression, were observed in the ICM, but not in the TE, of dormant embryos, and the levels decreased when the embryos were activated, consistent with changes in transcriptional activity. Substitution of histone H3.3, a variant of H3, for dominant H3.1 increased in activated embryos, suggesting that histone substitution is involved in inducing gene expression associated with activation. In the nucleus, H3.3 was mainly localized in the nucleoli of activated embryos but not in those of dormant ones. In contrast, there were no obvious differences in the trimethylation of histone H3K9 or the acetylation of histones H3K9, H3K18 and H4K12 between dormant and activated embryos. These results suggest that a decrease in H3K9 dimethylation contributes to the acquisition of implantation competence by releasing genes from suppression. In addition, histone H3.3 substitution seems to be involved in global gene activation and facilitates the prompt recovery of dormant blastocysts to the active state by inducing rRNA synthesis, resulting in an increase in translational activity. - Y Satoh; T Mano; H Tsuruga; R Masuda; T Matsuhashi; M Onuma; M Suzuki; N OhtaishiJAPANESE JOURNAL OF VETERINARY RESEARCH HOKKAIDO UNIV 50 (4) 195 - 199 0047-1917 2003/02 [Refereed]
To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes : Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB 3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido. - T Matsuhashi; R Masuda; T Mano; K Murata; A AiurzaniinZOOLOGICAL SCIENCE ZOOLOGICAL SOC JAPAN 18 (8) 1137 - 1143 0289-0003 2001/11 [Refereed]
Previous phylogenetic studies using mitochondrial DNA sequences of the brown bear Ursus arctos have separately defined two major lineages in Europe, three in Alaska, and three in Hokkaido Island of Japan. To reconstruct phylogenetic relationships among worldwide populations of the species, nucleotide sequences of the mitochondrial DNA control region and cytochrome b were determined for some additional subpopulations of Asia (Gobi and Tibetan), and then all the data including previously reported sequences were compared. The resultant phylogenetic trees showed that the worldwide populations could be grouped into at least five lineages. One of the five lineages had a wide distributional range covering Eurasia, Alaska, and central Hokkaido. Moreover, it is likely that populations from eastern Hokkaido and eastern Alaska are the direct derivatives of a single lineage. These results suggest that brown bears may have widely colonized Eurasia and North America from their original areas somewhere in Eurasia more than once. - T Matsuhashi; R Masuda; T Mano; MC YoshidaMOLECULAR BIOLOGY AND EVOLUTION SOC MOLECULAR BIOLOGY EVOLUTION 16 (5) 676 - 684 0737-4038 1999/05 [Refereed]
We investigated nucleotide sequences of the mitochondrial DNA control region to describe natural genetic variations and to assess the relationships between subpopulations of the brown bear Ursus arctos on Hokkaido Island, Japan. Using the polymerase chain reaction product-direct sequencing technique, partial sequences (about 930 bases) of the control region were determined for 56 brown bears sampled throughout Hokkaido Island. A sequence alignment revealed that the brown bear control region included a variable sequence on the 5' side and a repetitive region on the 3' side. Phylogenetic trees reconstructed from the 5' variable region (696-702 bases) exhibited 17 haplotypes, which were clustered into three groups (Clusters A, B, and C). The distribution of each group did not overlap with those of the others, and the three different areas were located in separate mountainous forests of Hokkaido Island. Furthermore, most of the phylogenetically close haplotypes within each group were distributed geographically close to each other. In addition, the 3' repetitive region (arrays of 10 bases) exhibited a much faster mutation rate than the 5' variable region, resulting in heteroplasmy. Such mitochondrial DNA divergence in each group could have occurred after the brown bears migrated from the continent to Hokkaido and became fixed in the different areas.
MISC
- 池上春香; 長野晟大; 松橋珠子; 永井宏平; 永井宏平; 宮本圭; 宮本圭; 加藤博己; 加藤博己; 松本和也; 松本和也; 松本和也 関西畜産学会報 (180) 2023
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- 東口奈那美; 本廣多胤; 池上春香; 松橋珠子; 松本和也; 吉廣卓哉 人工知能学会全国大会論文集(Web) 34th- 2020
- 塚本真伍; 池上春香; 松橋珠子; 松本和也; 吉廣卓哉 人工知能学会全国大会論文集(Web) 34th- 2020
- 坂本裕子; 奥野智美; LI Yang; 山本真理; 神谷拓磨; 越智浩介; 井橋俊哉; 辻本佳加理; 松橋珠子; 松本和也; GROSSE Robert; 宮本圭 Journal of Mammalian Ova Research 36- (1) S49 2019/04
- 守田昂太郎; 守田昂太郎; 守田昂太郎; 樋口智香; 安齋政幸; 安齋政幸; 松橋珠子; 松橋珠子; 黒坂哲; 黒坂哲; 加藤博己; 加藤博己; 三谷匡; 山縣一夫; 細井美彦; 宮本圭; 松本和也 日本実験動物学会総会講演要旨集(Web) 66th- 2019
- IHASHI Shunya; OCHI Kousuke; SAKAMOTO Yuko; TSUJIMOTO Kagari; KASAHARA Yoshito; MATSUHASHI Tamako; MATSUMOTO Kazuya; MIYAMOTO Kei; MORI Miki; IMASATO Yuma; KUSAKABE Haruna; KAJIKURI Naoaki; MATSUZAWA Yuka; KAMIYA Takuma; OKUNO Tomomi; YAMAMOTO Mari The Journal of Reproduction and Development Supplement 112- (0) P -98-P-98 2019
- 東口 奈那美; 本廣 多胤; 池上 春香; 松橋 珠子; 松本 和也; 吉廣 卓哉 情報処理学会関西支部支部大会講演論文集 2019
- 奥野智美; 樋口智香; 神谷拓磨; 山本真理; 越智浩介; 西野亜理紗; 井橋俊哉; 辻本佳加理; 松橋珠子; 安齋政幸; 黒坂哲; 三谷匡; 山縣一夫; 細井美彦; 松本和也; 宮本圭 Journal of Reproduction and Development 64- (Suppl Japanese Issue) j125 -70-P-70 2018/09
- 神谷拓磨; 本上遥; 久米健太; 樋口智香; 奥野智美; 山本真理; 越智浩介; 井橋俊哉; 辻本佳加理; 松橋珠子; 細井美彦; 松本和也; 宮本圭 Journal of Reproduction and Development 64- (Suppl Japanese Issue) j117 -54-P-54 2018/09
- 松橋珠子; 池上春香; 越智浩介; 大林賢伍; 佐野文美; 森隆史; 本廣多胤; 奥野智美; 神谷拓磨; 永井宏平; 宮本圭; 吉廣卓哉; 坂口慎一; 松本和也 日本畜産学会大会講演要旨 124th- 184 2018/03
- 越智浩介; 池上春香; 松橋珠子; 大林賢伍; 永井宏平; 坂口慎一; 松本和也 日本畜産学会大会講演要旨 124th- 183 2018/03
- 奥野智美; 山口壮輝; 樋口智香; 神谷拓磨; 山本真理; 越智浩介; 西野亜理紗; 井橋俊哉; 辻本佳加理; 坂本裕子; 松橋珠子; 安齋政幸; 黒坂哲; 三谷匡; 山縣一夫; 細井美彦; 松本和也; 宮本圭 日本分子生物学会年会プログラム・要旨集(Web) 41st- ROMBUNNO.2P‐0413 (WEB ONLY) 2018
- 樋口智香; 守田昂太郎; 山口壮輝; 松橋珠子; 永井宏平; 安齋政幸; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也 日本卵子学会誌 2- (1) S48 -S48 2017/04
- 守田昂太郎; 樋口智香; 山口壮輝; 松橋珠子; 松橋珠子; 永井宏平; 安齋政幸; 安齋政幸; 加藤博巳; 加藤博巳; 山縣一夫; 細井美彦; 宮本圭; 松本和也 日本卵子学会誌 2- (1) S47 -S47 2017/04
- 松橋珠子; 小林栄治; 池上春香; 森本学; 笹子奈々恵; 松本和也 日本畜産学会大会講演要旨 122nd- 145 2017/03
- H. Ozaki; T. Tamura; K. Fukazawa; Y. Uemoto; M. Nishio; E. Kobayashi; T. Matsuhashi; S. Maruyama; T. Honda; K. Oyama; S. Sasazaki; H. Mannen JOURNAL OF ANIMAL SCIENCE 94- 21 -21 2016/09
- 池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也 日本畜産学会大会講演要旨 121st- 243 2016/03
- Ikegami Haruka; Matsumoto Kazuya; Matsuhashi Tamako; Nagai Kouhei; Morimoto Manabu; Tsukaguchi Tomomasa; Yamaguchi Soki; Higuchi Chika; Morita Kotaro; Sakaguchi Shin-ichi Abstracts for Annual Meeting of Japanese Proteomics Society 2016- (0) 130 -130 2016
- 池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也 日本畜産学会大会講演要旨 120th- 89 2015/09
- 松橋珠子; 渡邊敏夫; 丸山新; 高原伸一; 山田英信; 杉本喜憲; 米山英雄 日本畜産学会大会講演要旨 119th- 169 2015/03
- 高須賀晶子; 齊藤陽介; 佐藤邦雄; 中村亮一; 小林宙; 松橋珠子; 鈴木晶夫; 瀬戸口浩二; 佐々木慎二; 早川磨紀男; 辻岳人; 小島孝敏 日本畜産学会大会講演要旨 119th- 171 -171 2015/03
- 丸山新; 松橋珠子; 向島幸司 日本畜産学会大会講演要旨 119th- 232 2015/03
- IARS異常症とは異なる虚弱子牛症候群の解析岡崎 惇朗; 平野 貴; 松橋 珠子; 佐々木 慎二; 鈴木 穣; 原 ひろみ; 杉本 喜憲; 半澤 恵 日本畜産学会大会講演要旨集 119回- 171 -171 2015/03
- 黒毛和種牛の脂肪交雑度を予測する血清マイクロRNAの探索池上 春香; 丹羽 尚人; 永井 宏平; 塚口 智将; 樋口 智香; 守田 昂太郎; 松橋 珠子; 小林 直彦; 松本 和也 日本畜産学会大会講演要旨集 119回- 185 -185 2015/03
- Ikegami Haruka; Nagai Kouhei; Matsuhashi Tamako; Kobayashi Naohiko; Higuchi Chika; Morita Koutaro; Uchibori Sho; Tsukaguchi Tomomasa; Kato Hiromi Abstracts for Annual Meeting of Japanese Proteomics Society 2015- (0) 234 -234 2015
- Tamako MATSUHASHI; Naohiko KOBAYASHI; Kenji TAKEDA; Manabu MORIMOTO; Koshi MUKOUJIMA; Yoshikazu SUGIMOTO; Hideo YONEYAMA Bulletin of the Gifu Prefectural Livestock Research Institute 14- (14) 1 -6 2014/07
- 黒毛和種牛の脂肪交雑度を予測するタンパク質バイオマーカーの探索池上 春香; 松橋 珠子; 赤尾 大樹; 武本 淳史; 永井 宏平; 内堀 翔; 小林 直彦; 松本 和也 日本畜産学会大会講演要旨集 118回- 151 -151 2014/03
- 黒毛和種のイソロイシルtRNA合成酵素(IARS)異常症劣性ホモ子牛と正常発育子牛の各月齢における血清アミノ酸濃度の比較鹿野 達也; 山崎 ふうこ; 富岡 美千子; 菊地 薫; 阿部 ふみ; 田高 恵; 松橋 珠子; 平野 貴; 杉本 喜憲; 渡辺 大作 日本獣医学会学術集会講演要旨集 156回- 331 -331 2013/08
- 黒毛和種繁殖雌牛におけるIARS遺伝子型と子牛生産性との関連松橋 珠子; 武田 賢治; 平野 貴; 西村 翔太; 佐々木 慎二; 杉本 喜憲; 北 和夫 日本畜産学会大会講演要旨集 116回- 145 -145 2013/03
- 丸山 新; 松橋 珠子; 小林 直彦; 中橋 良信; 口田 圭吾 肉用牛研究会報 94- 2013/01
- 石井淳; 山地啓太; 阪本嵩幸; 秋山賢斗; 上本吉伸; 笹子奈々恵; 小林栄治; 小林直彦; 松橋珠子; 丸山新; 松本大和; 笹崎晋史; 大山憲二; 万年英之 動物遺伝育種研究 40- (2) 73 2012/12
- 阪本嵩幸; 石井淳; 山地啓太; 早川きり; 上本吉伸; 笹子奈々恵; 高須賀晶子; 小林栄治; 小林直彦; 松橋珠子; 丸山新; 平野貴; 杉本喜憲; 松本大和; 笹崎晋史; 大山憲二; 万年英之 動物遺伝育種研究 40- (2) 72 2012/12
- 西牧孝洋; 揖斐隆之; 斯琴図雅; 小林直彦; 松橋珠子; 今井佳積; 松井茉由; 上村圭一; 衛藤央好; 渡邉直人; 藤田達男; 齊藤陽介; 小松智彦; 万年英之; 笹崎晋史; 国枝哲夫 動物遺伝育種研究 40- (2) 71 2012/12
- 池上 春香; 小林 直彦; 松橋 珠子; 武本 淳史; 吉廣 卓哉; 井上 悦子; 加藤 里恵; 加藤 博己; 田口 善智; 天野 朋子; 森本; 康一; 中川; 優#入谷; 明#松本 和也 日本畜産學會報 = The Japanese journal of zootechnical science 83- (3) 281 -290 2012/08
- 池上 春香; 小林 直彦; 松橋 珠子 日本畜産学会報 83- (3) 281 -290 2012/08
- Tamako MATSUHASHI; Akiko TAKASUGA; Shota NISHIMURA; Takashi HIRANO; Naohiko KOBAYASHI; Shin MARUYAMA; Koshi MUKOUJIMA; Yoshikazu SUGIMOTO; Kazuo KITA Bulletin of the Gifu Prefectural Livestock Research Institute 12- (12) 14 -21 2012/07
- MARUYAMA SHIN; MATSUHASHI TAMAKO; HOSHINO YOICHIRO; SAKAGUCHI SHIN'ICHI; SAEKI KAZUHIRO; KISHI MASAO; YAMAGUCHI SHIZUKO 日本官能評価学会誌 16- (1) 68 2012/04
- 佐々木慎二; 揖斐隆之; 松橋珠子; 池田省吾; 小林直彦; 杉本喜憲 日本畜産学会大会講演要旨 115th- 166 2012/03
- Proteomic Analysis of Bovine Serum: Biomarker Discovery for Developing Beef Production SystemsHaruka Ikegami; Atsushi Takemoto; Tamako Matsuhashi; Naohiko Kobayashi; Kazuo Kita; Koichi Morimoto; Kazuya Matsumoto Proceedings of the 3rd International Symposium on "Frontiers in Agriculture Proteome Research" 121 -123 2012/03 [Refereed][Invited]
- 丸山 新; 田中 等幸; 松橋 珠子; 小林 直彦; 星野 洋一郎; 棚橋 英樹 肉用牛研究会報 92- 2012/01
- 石井淳; 山地啓太; 阪本嵩幸; 上本吉伸; 笹子奈々恵; 小林栄治; 小林直彦; 松橋珠子; 丸山新; 松本大和; 笹崎晋史; 万年英之 動物遺伝育種研究 39- (2) 135 2011/12
- 西牧孝洋; 揖斐隆之; 田邉義弘; 宮崎義之; 小林直彦; 松橋珠子; 秋山敬孝; 吉田恵実; 今井佳積; 松井茉由; 上村圭一; 渡邉直人; 藤田達男; 齊藤陽介; 万年英之; 笹崎晋史; 国枝哲夫 動物遺伝育種研究 39- (2) 142 2011/12
- 佐々木慎二; 揖斐隆之; 松橋珠子; 池田省吾; 小林直彦; 渡邊敏夫; 杉本喜憲 動物遺伝育種研究 39- (2) 132 2011/12
- 丸山 新; 松橋 珠子; 星野 洋一郎; 坂口 慎一; 佐伯 和弘; 岸 昌生; 山口 静子 岐阜県畜産研究所研究報告 0- (11) 1 -4 2011/07
- 小林 直彦; 松橋 珠子; 坂口 慎一; 北 和夫; 国枝 哲夫 岐阜県畜産研究所研究報告 0- (11) 10 -19 2011/07
- 小林 直彦; 松橋 珠子; 丸山 新; 武田 賢治; 平野 貴; 高須賀 晶子; 坂口 慎一; 杉本 喜憲; 北 和夫 岐阜県畜産研究所研究報告 0- (11) 20 -26 2011/07
- 丸山 新; 小林 直彦; 松橋 珠子; 星野 洋一郎; 植田 拓也; 中橋 良信; 口田 圭吾 肉用牛研究会報 90- 2011/01
- 池上春香; 武本淳史; 松橋珠子; 森本康一; 小林直彦; 北和夫; 松本和也 日本プロテオーム学会大会プログラム・抄録集 2011 (Web)- 2011
- 佐々木慎二; 揖斐隆之; 渡邊敏夫; 松橋珠子; 中藤由紀; 小林直彦; 杉本喜憲 動物遺伝育種研究 38- (2) 131 2010/12
- 宮坂 卓; 竹嶋 伸之輔; 松本 有生; 小林 直彦; 松橋 珠子; 石橋 和樹; 泉對 博; 間 陽子 MHC: Major Histocompatibility Complex 17- (2) 177 -177 2010/08
- Tamako MATSUHASHI; Shin MARUYAMA; Yoshinobu UEMOTO; Naohiko KOBAYASHI; Hideyuki MANNEN; Tsuyoshi ABE; Shinichi SAKAGUCHI; Eiji KOBAYASHI; Tsutomu KATO Bulletin of the Gifu Prefectural Livestock Research Institute 10- (10) 11 -22 2010/07
- ウシ主要組織適合抗原(MHC)クラスII遺伝子のハプロタイプの解析宮坂 卓; 竹嶋 伸之輔; 松本 有生; 神馬 繭子; 松橋 珠子; 小林 直彦; 泉對 博; 間 陽子 日本獣医学会学術集会講演要旨集 149回- 259 -259 2010/03
- 丸山 新; 松橋 珠子; 小林 直彦; 星野 洋一郎; 植田 拓也; 坂口 慎一; 中橋 良信; 口田 圭吾 肉用牛研究会報 88- 2010/01
- 中橋良信; 松橋珠子; 丸山新; 小林直彦; 坂口慎一; 万年英之; 増田豊; 口田圭吾 日本畜産学会大会講演要旨 112th- 2010
- 池上春香; 松橋珠子; 小林直彦; 大谷健; 森本康一; 松本和也 日本畜産学会大会講演要旨 112th- 2010
- 松本和也; 池上春香; 松橋珠子; 大谷健; 小林直彦; 森本康一 日本畜産学会大会講演要旨 112th- 2010
- 小林 直彦; 平野 貴; 松橋 珠子; 丸山 新; 星野 洋一郎; 傍島 英雄; 林 登; 坂口 慎一; 加藤 勉; 酒井 謙司; 杉本 喜憲 岐阜県畜産研究所研究報告 (9) 8 -17 2009/07
- Tamako MATSUHASHI; Shin MARUYAMA; Naohiko KOBAYASHI; Tsuyoshi ABE; Shinichi SAKAGUCHI; Tsutomu KATO Bulletin of the Gifu Prefectural Livestock Research Institute 9- (9) 18 -25 2009/07
- 池上春香; 園陽平; 永井宏平; 吉廣卓哉; 井上悦子; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 松本和也 日本畜産学会大会講演要旨 111th- 2009
- 池上春香; 池上春香; 園陽平; 園陽平; 永井宏平; 永井宏平; 吉廣卓哉; 井上悦子; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 森本康一; 松本和也; 松本和也 日本プロテオーム学会大会プログラム・抄録集 2009 (Web)- 2009
- 神馬 繭子; 竹嶋 伸之輔; 遠藤 大二; 宮坂 卓; 小林 直彦; 松橋 珠子; 的場 和弘; 間 陽子 動物遺伝育種研究 36- (2) 233 -233 2008/12
- NAGAI Kouhei; YOSHIHIRO Takuya; INOUE Etsuko; IKEGAMI Haruka; SONO Youhei; KAWAJI Hideya; KOBAYASHI Naohiko; MATSUHASHI Tamako; OHTANI Tsuyoshi; MORIMOTO Koichi; NAKAGAWA Masaru; IRITANI Akira; MATSUMOTO Kazuya Nihon Chikusan Gakkaiho 79- (4) 467 -481 2008/11
- 松橋 珠子 畜産技術 642- (642) 7 -11 2008/11
- 常石 英作; 丸山 新; 松橋 珠子 西日本畜産学会報 51- 79 -81 2008/07
- HAYASHI NOBORU; HOSHINO YOICHIRO; MATSUHASHI TAMAKO; KOBAYASHI NAOHIKO; ABE YUKI; IWAMOTO TAISAKU; TANIGUCHI TOSHIHITO; MATSUMOTO KAZUYA; HOSOI YOSHIHIKO; IRITANI AKIRA; NAGAO YOSHIKAZU; SAEKI KAZUHIRO 日本はい移植学雑誌 30- (1) 57 2008/01
- 池上春香; 園陽平; 永井宏平; 吉廣卓哉; 井上悦子; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 松本和也 生化学 2008
- SHI Linjing; 井上悦子; 吉廣卓哉; 永井宏平; 池上春香; 松橋珠子; 小林直彦; 森本康一; 松本和也; 中川優 情報処理学会全国大会講演論文集 70th- (1) 2008
- 池上春香; 永井宏平; 吉廣卓哉; 井上悦子; 園陽平; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 森本康一; 松本和也; 松本和也 質量分析総合討論会講演要旨集 56th- 2008
- 池上春香; 永井宏平; 吉廣卓哉; 園陽平; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 松本和也 日本畜産学会大会講演要旨 109th- 2008
- 常石 英作; 丸山 新; 松橋 珠子; 小林 直彦; 坂口 慎一; 神谷 充 西日本畜産学会報 51- 79 -81 2008
- 池上春香; 永井宏平; 吉廣卓哉; 園陽平; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 松本和也 日本畜産学会大会講演要旨 108th- 4 2007/09
- 小林 直彦; 松橋 珠子; 星野 洋一郎 岐阜県畜産研究所研究報告 (7) 1 -6 2007/07
- Tamako MATSUHASHI; Shin MARUYAMA; Eisaku TSUNEISHI; Naohiko KOBAYASHI; Noboru HAYASHI; Yoichiro HOSHINO; Kenji SAKAI Bulletin of the Gifu Prefectural Livestock Research Institute 7- (7) 7 -13 2007/07
- 池上春香; 永井宏平; 吉廣卓哉; 園陽平; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 松本和也 生化学 2007
- 池上春香; 永井宏平; 吉廣卓哉; 園陽平; 小林直彦; 松橋珠子; 大谷健; 中川優; 森本康一; 森本康一; 松本和也; 松本和也 日本プロテオーム学会大会プログラム・抄録集 2007 (Web)- 2007
- 常石 英作; 小林 直彦; 丸山 新; 松橋 珠子; 折戸 秀樹; 神谷 充 西日本畜産学会報 50- 119 -120 2007
- Y Satoh; T Mano; H Tsuruga; R Masuda; T Matsuhashi; M Onuma; M Suzuki; N Ohtaishi JAPANESE JOURNAL OF VETERINARY RESEARCH 51- (2) 121 -121 2003/08
Lectures, oral presentations, etc.
- 黒毛和種肥育牛の枝肉成績を予測する血中バイオマーカーマイクロRNAの探索 [Not invited]笠原喜斗; 笹部冴子; 池上春香; 大林賢伍; 向島幸司; 越智浩介; 宮本圭; 松橋珠子; 松本和也; 松本和也; 松本和也日本畜産学会大会講演要旨 2019/03
- 畜産領域へのリキッドバイオプシーの展開 3:枝肉成績を肥育中に予測するウシ血清バイオマーカータンパク質の探索 [Not invited]松橋珠子; 池上春香; 越智浩介; 本廣多胤; 東口奈那美; 大林賢伍; 向島幸司; 高取等; 邨上正幸; 渡邉智; 笠原喜斗; 永井宏平; 宮本圭; 吉廣卓哉; 松本和也; 松本和也; 松本和也日本畜産学会大会講演要旨 2019/03
- 黒毛和種の枝肉形質と関連する血中バイオマーカー候補マイクロRNAの探索 [Not invited]松橋 珠子; 柴田成基; 笹部冴子; 池上 春香; 越智浩介; 大林賢伍; 永井 宏平; 宮本 圭; 松本 和也第68回関西畜産学会 2018/09
- 畜産領域へのリキッドバイオプシーの展開 1:牛の血清中タンパク質の同時定量解析方法の確立 [Not invited]越智浩介; 池上春香; 松橋珠子; 大林賢伍; 永井宏平; 坂口慎一; 松本和也日本畜産学会大会講演要旨 2018/03
- 畜産領域へのリキッドバイオプシーの展開 2:牛の枝肉成績を肥育中に予測する血清バイオマーカータンパク質の探索 [Not invited]松橋珠子; 池上春香; 越智浩介; 大林賢伍; 佐野文美; 森隆史; 本廣多胤; 奥野智美; 神谷拓磨; 永井宏平; 宮本圭; 吉廣卓哉; 坂口慎一; 松本和也日本畜産学会大会講演要旨 2018/03
- 黒毛和種におけるAnnexin A5遺伝子多型と繁殖成績との関連性の検討 [Not invited]松橋珠子; 池上春香; 米澤康滋; 大林賢伍; 越智浩介; 奥野智美; 神谷拓磨; 宮本 圭; 坂口慎一; 松本和也第67回関西畜産学会大阪大会 2017/09
- 黒毛和種のプロテオーム解析から検出されたAnnexinA5遺伝子多型と経済形質との関連 [Not invited]松橋珠子; 小林栄治; 池上春香; 森本学; 笹子奈々恵; 松本和也日本畜産学会大会講演要旨 2017/03
- 黒毛和種去勢牛の枝肉成績と関連する血清中バイオマーカー候補マイクロRNAの探索 [Not invited]松橋 珠子; 池上 春香; 森本 学; 永井 宏平; 山口 壮輝; 塚口 智将; 樋口 智香; 守田 昂太郎; 宮本 圭; 坂口 慎一; 松本 和也平成28年度(第66回)関西畜産学会香川大会 2016/10
- 黒毛和種去勢牛の枝肉形質を生体評価するバイオマーカー候補タンパク質の解析 [Not invited]池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也日本畜産学会大会講演要旨 2016/03
- 黒毛和種牛の枝肉形質を予測するバイオマーカー候補タンパク質の肥育期間中の動態 [Not invited]池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也日本畜産学会大会講演要旨 2015/09
- 黒毛和種リブロースの各筋肉の遊離アミノ酸含量について [Not invited]丸山新; 松橋珠子; 向島幸司日本畜産学会大会講演要旨 2015/03
- 骨格異常と枝肉重量QTL(CW‐3)は体高の増加と胸幅の減少によって説明される [Not invited]高須賀晶子; 齊藤陽介; 佐藤邦雄; 中村亮一; 小林宙; 松橋珠子; 鈴木晶夫; 瀬戸口浩二; 佐々木慎二; 早川磨紀男; 辻岳人; 小島孝敏日本畜産学会大会講演要旨 2015/03
- 黒毛和種肥育牛を訓練集団とした枝肉形質ゲノム育種価評価の試み [Not invited]松橋珠子; 渡邊敏夫; 丸山新; 高原伸一; 山田英信; 杉本喜憲; 米山英雄日本畜産学会大会講演要旨 2015/03
- 黒毛和種牛の脂肪交雑度を予測する血清マイクロRNAの探索 [Not invited]池上 春香; 丹羽 尚人; 永井 宏平; 塚口 智将; 樋口 智香; 守田 昂太郎; 松橋 珠子; 小林 直彦; 松本 和也日本畜産学会大会講演要旨集 2015/03
- IARS異常症とは異なる虚弱子牛症候群の解析 [Not invited]岡崎 惇朗; 平野 貴; 松橋 珠子; 佐々木 慎二; 鈴木 穣; 原 ひろみ; 杉本 喜憲; 半澤 恵日本畜産学会大会講演要旨集 2015/03
- 遺伝病 ~もう一度見直しましょう!~ [Invited]松橋 珠子岐阜県家畜人工授精師協会岐阜支部研修会 2014/03
- 黒毛和種牛の脂肪交雑度を予測するタンパク質バイオマーカーの探索 [Not invited]池上 春香; 松橋 珠子; 赤尾 大樹; 武本 淳史; 永井 宏平; 内堀 翔; 小林 直彦; 松本 和也日本畜産学会大会講演要旨集 2014/03
- Nagai Kouhei; Ikegami Haruka; Matsuhashi Tamako; Takemoto Atsushi; Minakata Yusuke; Higuchi Chika; Morita Koutaro; Kobayashi Naohiko; Matsumoto KazuyaAbstracts for Annual Meeting of Japanese Proteomics Society 2014
- 黒毛和種のイソロイシルtRNA合成酵素(IARS)異常症劣性ホモ子牛と正常発育子牛の各月齢における血清アミノ酸濃度の比較 [Not invited]鹿野 達也; 山崎 ふうこ; 富岡 美千子; 菊地 薫; 阿部 ふみ; 田高 恵; 松橋 珠子; 平野 貴; 杉本 喜憲; 渡辺 大作日本獣医学会学術集会講演要旨集 2013/08
- 黒毛和種繁殖雌牛におけるIARS遺伝子型と子牛生産性との関連 [Not invited]松橋 珠子; 武田 賢治; 平野 貴; 西村 翔太; 佐々木 慎二; 杉本 喜憲; 北 和夫日本畜産学会大会講演要旨集 2013/03
- 飛騨牛らしさに関する形質の遺伝的パラメータの推定 [Not invited]丸山 新; 松橋 珠子; 小林 直彦; 中橋 良信; 口田 圭吾肉用牛研究会報 2013/01
- 子牛虚弱症候群(WCS) について [Invited]松橋 珠子飛騨家畜人工授精師協会技術研修会 2012/09
- 飛騨牛の生産性に関与する遺伝子の解明 [Invited]松橋 珠子岐阜県家畜人工授精師協会岐阜支部技術研修会 2012/03
- Ikegami Haruka; Matsuhashi Tamako; Takemoto Atsushi; Nagai Kouhei; Higuchi Tomoka; Morita Koutaro; Kobayashi Naohiko; Matsumoto KazuyaAbstracts for Annual Meeting of Japanese Proteomics Society 2012
- 飛騨牛の生産性に関与する遺伝子の解明 [Invited]松橋 珠子岐阜県家畜人工授精師協会技術研修会 2011/11
- 飛騨牛の生産性に関与する遺伝子の解明 [Invited]松橋 珠子西濃人工授精師会研修会 2011/09
- 飛騨牛の生産性に関与する遺伝子の解明 [Invited]松橋 珠子岐阜県獣医師会県職部会 2011/06
- Ikegami Haruka; Takemoto Atsushi; Matsuhashi Tamako; Morimoto Koichi; Kobayashi Naohiko; Kita Kazuo; Matsumoto KazuyaAbstracts for Annual Meeting of Japanese Proteomics Society 2011
- エゾヒグマの遺伝学的特性と生物地理 [Invited]松橋 珠子平成22年度大学改革推進等補助金(大学改革推進事業)に もとづく自治体関係者向け講演会 2010/12
- 裏山系ハンターの日常:生活の中のイノシシ猟 [Invited]松橋 珠子2010年度日本哺乳類学会自由集会 ニッポンのハンターを絶滅から救え! 2010/09
- A System for Brand Beef-cattle (Hidagyu) Breeders to Support Selecting Well-Suited Sires [Not invited]SHI Linjing; INOUE Etsuko; YOSHIHIRO Takuya; NAGAI Kouhei; IKEGAMI Haruka; KOBAYASHI Naohiko; MATSUMOTO Kazuya; NAKAGAWA Masaru全国大会講演論文集 2008/03
Affiliated academic society
Research Themes
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2021/04 -2025/03Author : 猪島 康雄; 岡田 彩加; 高島 茂雄; 松橋 珠子
- 日本学術振興会:科学研究費助成事業Date (from‐to) : 2020/04 -2024/03Author : 松本 和也; 根本 充貴; 松橋 珠子本研究では、我々は肉用牛の肥育期間中に経時的に採取した血清を対象とした質量分析機を用いた定量プロテオミクスSWATH-MS(Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry)解析情報に基づき世界に先駆けて実施している「血清中バイオマーカータンパク質の動態と種類に関する情報から肉用牛の肥育状態を肥育期間中に生体評価するシステム」の開発研究を積み上げている。これは、畜産学と情報科学との異分野融合研究によって、肉用牛の産肉形質を生体評価するシステムの開発と、その結果得られる血清バイオマーカータンパク質の情報に基づく産肉制御の分子機構の統合的理解を目的とするものである。さらに、研究の成果は、これまでその詳細が不明であった肥育期間中の肉用牛における産肉形質の発育に関与する分子機構の解明に資する新しい知見の獲得が期待される。今後、本研究成果の生体評価システムが肉用牛の生産現場に実装された場合、農家が持つ経験値に依存した農業経営から科学的根拠に基づく農業経営への転換の基盤構築に貢献できる。 2年目の成果として、初年度に基盤構築した、日本の地域性の異なる3地域と全国レベルの肉用牛を扱っている研究機関の協力を得て、供試された出荷時の肉用牛(黒毛和種去勢)の枝肉成績の情報と肥育期間中のウシ血清サンプルに基づいた肉用牛の産肉形質の発育状態や肥育状態を生体評価するシステムを用いて、血清バイオマーカータンパク質の情報を基盤とした産肉制御の分子機構の統合的理解を目指して分子間パスウェイネットワーク解析ソフト(IPA)を導入し、産肉形質の形成過程における血清バイオマーカータンパク質の動態から、脂肪交雑の発育シミュレーションモデルを作成した。
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2016/04 -2018/03Author : Matsumoto KazuyaIn this study, relations between carcass traits and exosomal miRNA expression profiles in serum were analyzed to identify circulating exosomal miRNA biomarkers for predicting carcass traits and meat quality characteristics of Japanese Black cattle by bioinformatics analysis. Eleven secreted microRNAs were listed as biomarker candidates as a result of microRNA microarray analysis using serum of fattening cattle. Subsequently, reproducibility was confirmed for the two biomarker candidate microRNAs by the association analysis between the amount of blood microRNA quantified by real-time PCR analysis and the carcass traits. Those selected microRNAs were inferred to affect the expression of genes related to differentiation and development of cells constituting muscle tissue by computational searches of existing public databases.