MIYAMOTO Kei

    Department of Genetic Engineering Associate Professor
Last Updated :2024/03/24

Researcher Information

J-Global ID

Research Interests

  • Gene expression   Epigenetics   Nuclear transfer   Reprogramming   Molecular and developmental biology   

Research Areas

  • Life sciences / Zoological sciences

Academic & Professional Experience

  • 2020/04 - Today  Kindai UniversityFaculty of Biology-Oriented Science and TechnologyAssociate Professor
  • 2015/04 - 2020/03  Kindai UniversityFaculty of Biology-Oriented Science and Technology講師
  • 2012/04 - 2015/03  ケンブリッジ大学ウォルソンカレッジフェロー
  • 2012/04 - 2015/03  ハーチェルスミスリサーチフェロー
  • 2009/04 - 2015/03  英国ケンブリッジ大学ガードン研究所ガードン研究室博士研究員
  • 2010/04 - 2012/03  日本学術振興会海外特別研究員
  • 2007/04 - 2009/03  日本学術振興会特別研究員(DC2)

Education

  • 2006/04 - 2009/03  京都大学農学研究科博士後期課程 修了
  • 2004/04 - 2006/03  京都大学農学研究科博士前期課程 修了
  • 2000/04 - 2004/03  Kyoto University  Faculty of Agriculture

Association Memberships

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN   SOCIETY FOR REPRODUCTION AND DEVELOPMENT   日本胚移植学会   JAPAN SOCIETY FOR OVA RESEARCH   

Published Papers

  • Shunya Ihashi; Mizuto Hamanaka; Masaya Kaji; Ryunosuke Mori; Shuntaro Nishizaki; Miki Mori; Yuma Imasato; Kimiko Inoue; Shogo Matoba; Narumi Ogonuki; Atsushi Takasu; Misaki Nakamura; Kazuya Matsumoto; Masayuki Anzai; Atsuo Ogura; Masahito Ikawa; Kei Miyamoto
    Life science alliance 6 (11) 2023/11 [Refereed]
     
    Differentiated cell nuclei can be reprogrammed after nuclear transfer (NT) to oocytes and the produced NT embryos can give rise to cloned animals. However, development of NT embryos is often hampered by recurrent reprogramming failures, including the incomplete activation of developmental genes, yet specific genes responsible for the arrest of NT embryos are not well understood. Here, we searched for developmentally important genes among the reprogramming-resistant H3K9me3-repressed genes and identified Alyref and Gabpb1 by siRNA screening. Gene knockout of Alyref and Gabpb1 by the CRISPR/Cas9 system resulted in early developmental arrest in mice. Alyref was needed for the proper formation of inner cell mass by regulating Nanog, whereas Gabpb1 deficiency led to apoptosis. The supplement of Alyref and Gabpb1 mRNA supported efficient preimplantation development of cloned embryos. Alyref and Gabpb1 were silenced in NT embryos partially because of the repressed expression of Klf16 by H3K9me3. Thus, our study shows that the H3K9me3-repressed genes contain developmentally required genes, and the incomplete activation of such genes results in preimplantation arrest of cloned embryos.
  • 坂上 凜; 宮川 靖基; 宮本 圭
    Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University (50) 33 - 43 2023/03 [Refereed]
  • Tanaka Masahito; Rin Sakanoue; Atsushi Takasu; Naoko Watanabe; Yuta Shimamoto; Kei Miyamoto
    bioRxiv Cold Spring Harbor Laboratory 2023/02 
    Abstract Upon fertilization, germ cells are reprogrammed to acquire the ability to develop into an entire organism. Whereas extensive studies have focused on epigenetic reprogramming of chromatin states during development, changes of the nucleus that surrounds chromatin are ill-defined. Here, we show that nuclei become structurally and mechanically vulnerable at the 2-cell stage during mouse embryonic development. The 2-cell stage nuclei are extraordinarily plastic and deformable in contrast to those of 1-cell and 4-cell stages. The mechanically vulnerable nuclear state is attained by autophagy-mediated loss of lamin B1 from the nuclear membrane. This developmentally programmed lamin B1 dynamics is required for chromatin organization and major zygotic genome activation. We thus demonstrate that structural reprogramming of nuclei is a major determinant of embryonic gene expression and acquisition of totipotency.
  • Junko Tomikawa; Christopher A. Penfold; Rena Hatakeyama; Kei Miyamoto
    STAR Protocols Elsevier BV 3 (2) 101284 - 101284 2666-1667 2022/06 [Refereed][Invited]
  • Sivagami Gunasekaran; Yasuki Miyagawa; Kei Miyamoto
    Current opinion in cell biology 76 102100 - 102100 2022/05 [Refereed][Invited]
     
    Dynamic assembly and disassembly of actin proteins play a key role in the cytoskeleton, but the cellular functions of actin are not only restricted to the cytoplasmic compartment. Recent studies have shown that actin spatiotemporally changes its polymerized state in the nucleus as well and such dynamic nature of actin is relevant to key nuclear events including gene expression, DNA damage response and chromatin organization. In this review, we highlight emerging roles of actin in the nuclear compartment especially in the context of embryonic development and cellular differentiation. We first explain how the actin nucleoskeleton can be formed and function in cells. Notably, nuclear actin dynamics are greatly altered when cell fates change, such as after fertilization and T cell differentiation. We discuss how the dynamic actin nucleoskeleton contributes to accomplishing developmental programs.
  • Hiroki Takeuchi; Mari Yamamoto; Megumi Fukui; Akihiro Inoue; Tadashi Maezawa; Mikiko Nishioka; Eiji Kondo; Tomoaki Ikeda; Kazuya Matsumoto; Kei Miyamoto
    Reproductive medicine and biology 21 (1) e12464  2022/05 [Refereed]
     
    PURPOSE: In vitro maturation (IVM) of human oocytes offers an invaluable opportunity for infertility treatment. However, in vitro matured oocytes often show lower developmental abilities than their in vivo counterparts, and molecular mechanisms underlying successful maturation remain unclear. In this study, we investigated gene expression profiles of in vitro matured oocytes at the single-cell level to gain mechanistic insight into IVM of human oocytes. METHODS: Human oocytes were retrieved by follicular puncture and in vitro matured. In total, 19 oocytes from 11 patients were collected and subjected to single-cell RNA-seq analyses. RESULTS: Global gene expression profiles were similar among oocytes at the same maturation stage, while a small number of oocytes showed distinct transcriptomes from those at the corresponding maturation stage. Differential gene expression analysis identified hundreds of transcripts that dynamically altered their expression during IVM, and we revealed molecular pathways and upstream regulators that may govern oocyte maturation. Furthermore, oocytes that were delayed in their maturation showed distinct transcriptomes. Finally, we identified genes whose transcripts were enriched in each stage of oocyte maturation. CONCLUSIONS: Our work uncovers transcriptomic changes during human oocyte IVM and the differential gene expression profile of each oocyte.
  • Shunya Ihashi; Mizuto Hamanaka; Masaya Kaji; Miki Mori; Yuma Imasato; Misaki Nakamura; Masayuki Anzai; Kazuya Matsumoto; Masahito Ikawa; Kei Miyamoto
    bioRxiv Cold Spring Harbor Laboratory 2022/04 
    SUMMARY Differentiated cell nuclei can be reprogrammed after nuclear transfer (NT) to oocytes and the produced NT embryos can give rise to cloned animals. However, development of NT embryos is often hampered by recurrent reprogramming failures, including the incomplete activation of developmental genes, yet specific genes responsible for the arrest of NT embryos are not well understood. Here, we searched for developmentally important genes among the reprogramming-resistant H3K9me3-repressed genes, and identified Alyref and Gabpb1 by siRNA screening. Gene knockout of Alyref and Gabpb1 by the CRISPR/Cas9 system resulted in early developmental arrest in mice. Single embryo RNA-seq revealed that Alyref is needed for the formation of inner cell mass. The supplement of Alyref and Gabpb1 by mRNA injection supported efficient preimplantation development of cloned embryos. Thus, our study shows that the H3K9me3-repressed genes contain developmentally required genes and the incomplete activation of such genes results in preimplantation arrest of cloned embryos.
  • Junko Tomikawa; Christopher A Penfold; Takuma Kamiya; Risa Hibino; Ayumi Kosaka; Masayuki Anzai; Kazuya Matsumoto; Kei Miyamoto
    iScience 24 (11) 103290  2021/11 [Refereed]
     
    Nuclear transfer systems represent the efficient means to reprogram a cell and in theory provide a basis for investigating the development of endangered species. However, conventional nuclear transfer using oocytes of laboratory animals does not allow reprogramming of cross-species nuclei owing to defects in cell divisions and activation of embryonic genes. Here, we show that somatic nuclei transferred into mouse four-cell embryos arrested at the G2/M phase undergo reprogramming toward the embryonic state. Remarkably, genome-wide transcriptional reprogramming is induced within a day, and ZFP281 is important for this replication-free reprogramming. This system further enables transcriptional reprogramming of cells from Oryx dammah, now extinct in the wild. Thus, our findings indicate that arrested mouse embryos are competent to induce intra- and cross-species reprogramming. The direct induction of embryonic transcripts from diverse genomes paves a unique approach for identifying mechanisms of transcriptional reprogramming and genome activation from a diverse range of species.
  • Satoshi Kamimura; Kimiko Inoue; Eiji Mizutani; Jin-Moon Kim; Hiroki Inoue; Narumi Ogonuki; Kei Miyamoto; Shunya Ihashi; Nobuhiko Itami; Teruhiko Wakayama; Akihiro Ito; Norikazu Nishino; Minoru Yoshida; Atsuo Ogura
    Biology of reproduction 105 (2) 543 - 553 2021/08 [Refereed]
     
    In mammalian cloning by somatic cell nuclear transfer (SCNT), the treatment of reconstructed embryos with histone deacetylase (HDAC) inhibitors improves efficiency. So far, most of those used for SCNT are hydroxamic acid derivatives-such as trichostatin A-characterized by their broad inhibitory spectrum. Here, we examined whether mouse SCNT efficiency could be improved using chlamydocin analogues, a family of newly designed agents that specifically inhibit class I and IIa HDACs. Development of SCNT-derived embryos in vitro and in vivo revealed that four out of five chlamydocin analogues tested could promote the development of cloned embryos. The highest pup rates (7.1-7.2%) were obtained with Ky-9, similar to those achieved with trichostatin A (7.2-7.3%). Thus, inhibition of class I and/or IIa HDACs in SCNT-derived embryos is enough for significant improvements in full-term development. In mouse SCNT, the exposure of reconstructed oocytes to HDAC inhibitors is limited to 8-10 h because longer inhibition with class I inhibitors causes a two-cell developmental block. Therefore, we used Ky-29, with higher selectivity for class IIa than class I HDACs for longer treatment of SCNT-derived embryos. As expected, 24-h treatment with Ky-29 up to the two-cell stage did not induce a developmental block, but the pup rate was not improved. This suggests that the one-cell stage is a critical period for improving SCNT cloning using HDAC inhibitors. Thus, chlamydocin analogues appear promising for understanding and improving the epigenetic status of mammalian SCNT-derived embryos through their specific inhibitory effects on HDACs.
  • Kohtaro Morita; Yuki Hatanaka; Shunya Ihashi; Masahide Asano; Kei Miyamoto; Kazuya Matsumoto
    Scientific reports 11 (1) 10146  2021/05 [Refereed]
     
    Paternal genome reprogramming, such as protamine-histone exchange and global DNA demethylation, is crucial for the development of fertilised embryos. Previously, our study showed that one of histone arginine methylation, asymmetrically dimethylated histone H3R17 (H3R17me2a), is necessary for epigenetic reprogramming in the mouse paternal genome. However, roles of histone arginine methylation in reprogramming after fertilisation are still poorly understood. Here, we report that H3R2me2s promotes global transcription at the 1-cell stage, referred to as minor zygotic genome activation (ZGA). The inhibition of H3R2me2s by expressing a histone H3.3 mutant H3.3R2A prevented embryonic development from the 2-cell to 4-cell stages and significantly reduced global RNA synthesis and RNA polymerase II (Pol II) activity. Consistent with this result, the expression levels of MuERV-L as minor ZGA transcripts were decreased by forced expression of H3.3R2A. Furthermore, treatment with an inhibitor and co-injection of siRNA to PRMT5 and PRMT7 also resulted in the attenuation of transcriptional activities with reduction of H3R2me2s in the pronuclei of zygotes. Interestingly, impairment of H3K4 methylation by expression of H3.3K4M resulted in a decrease of H3R2me2s in male pronuclei. Our findings suggest that H3R2me2s together with H3K4 methylation is involved in global transcription during minor ZGA in mice.
  • Junko Tomikawa; Kei Miyamoto
    The FEBS journal 2021/04 [Refereed][Invited]
     
    The regulation of gene expression is a critical process for establishing and maintaining cellular identity. Gene expression is controlled through a chromatin-based mechanism in the nucleus of eukaryotic cells. Recent studies suggest that chromatin accessibility and the higher-order structure of chromatin affect transcriptional outcome. This is especially evident when cells change their fate during development and nuclear reprogramming. Furthermore, non-chromosomal contents of the cell nucleus, namely nucleoskeleton proteins, can also affect chromatin and nuclear structures, resulting in transcriptional alterations. Here, we review our current mechanistic understanding about how chromatin and nuclear structures impact transcription in the course of embryonic development, cellular differentiation and nuclear reprogramming, and also discuss unresolved questions that remain to be addressed in the field.
  • Kei Miyamoto; Masahiko Harata
    Journal of biochemistry 169 (3) 237 - 241 2021/01 [Refereed][Invited]
     
    The eukaryotic nucleus shows organized structures of chromosomes, transcriptional components and their associated proteins. It has been believed that such a dense nuclear environment prevents the formation of a cytoskeleton-like network of protein filaments. However, accumulating evidence suggests that the cell nucleus also possesses structural filamentous components to support nuclear organization and compartments, which are referred to as nucleoskeleton proteins. Nucleoskeleton proteins including lamins and actin influence nuclear dynamics including transcriptional regulation, chromatin organization and DNA damage responses. Furthermore, these nucleoskeleton proteins play a pivotal role in cellular differentiation and animal development. In this commentary, we discuss how nucleoskeleton-based regulatory mechanisms orchestrate nuclear dynamics.
  • Yuto Takahashi; Shogo Hiratsuka; Nanako Machida; Daisuke Takahashi; Junpei Matsushita; Pavel Hozak; Tom Misteli; Kei Miyamoto; Masahiko Harata
    Nucleus (Austin, Tex.) 11 (1) 250 - 263 2020/12 [Refereed]
     
    Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disorder caused by a mutation of lamin A, which contributes to nuclear architecture and the spatial organization of chromatin in the nucleus. The expression of a lamin A mutant, named progerin, leads to functional and structural disruption of nuclear organization. Since progerin lacks a part of the actin-binding site of lamin A, we hypothesized that nuclear actin dynamics and function are altered in HGPS cells. Nuclear F-actin is required for the organization of nuclear shape, transcriptional regulation, DNA damage repair, and activation of Wnt/β-catenin signaling. Here we show that the expression of progerin decreases nuclear F-actin and impairs F-actin-regulated transcription. When nuclear F-actin levels are increased by overexpression of nuclear-targeted actin or by using jasplakinolide, a compound that stabilizes F-actin, the irregularity of nuclear shape and defects in gene expression can be reversed. These observations provide evidence for a novel relationship between nuclear actin and the etiology of HGPS.
  • Taiki Shindo; Shunya Ihashi; Yuko Sakamoto; Tomomi Okuno; Junko Tomikawa; Kei Miyamoto
    Journal of biochemistry 2020/11 [Refereed][Invited]
     
    Actin in the nucleus, referred to as nuclear actin, is involved in a variety of nuclear events. Nuclear actin is present as a globular (G-actin) and filamentous form (F-actin), and dynamic assembly/disassembly of nuclear actin profoundly affects nuclear functions. However, it is still challenging to observe endogenous nuclear F-actin. Here, we present a condition to visualize endogenous nuclear F-actin of mouse zygotes using different fixation methods. Zygotes fixed with paraformaldehyde and treated with fluorescently conjugated phalloidin show both short and long actin filaments in their pronuclei. Short nuclear actin filaments are characteristic of phalloidin staining, rather than the consequence of severing actin filaments by the fixation process, since long nuclear actin filaments probed with the nuclear actin chromobody are not disassembled into short filaments after fixation with paraformaldehyde. Furthermore, we find that nuclear actin assembly is impaired after somatic cell nuclear transfer (SCNT), suggesting abnormal nucleoskeleton structures in SCNT embryos. Taken together, our presented method for visualizing nuclear F-actin with phalloidin can be used to observe the states of nuclear actin assembly, and revealed improper reprogramming of actin nucleoskeleton structures in cloned mouse embryos.
  • Tomomi Okuno; Wayne Yang Li; Yu Hatano; Atsushi Takasu; Yuko Sakamoto; Mari Yamamoto; Zenki Ikeda; Taiki Shindo; Matthias Plessner; Kohtaro Morita; Kazuya Matsumoto; Kazuo Yamagata; Robert Grosse; Kei Miyamoto
    Cell reports 31 (13) 107824 - 107824 2020/06 [Refereed]
     
    After fertilization, sperm and oocyte nuclei are rapidly remodeled to form swollen pronuclei (PN) in mammalian zygotes, and the proper formation and function of PN are key to producing totipotent zygotes. However, how mature PN are formed has been unclear. We find that filamentous actin (F-actin) assembles in the PN of mouse zygotes and is required for fully functional PN. The perturbation of nuclear actin dynamics in zygotes results in the misregulation of genes related to genome integrity and abnormal development of mouse embryos. We show that nuclear F-actin ensures DNA damage repair, thus preventing the activation of a zygotic checkpoint. Furthermore, optogenetic control of cofilin nuclear localization reveals the dynamically regulated F-actin nucleoskeleton in zygotes, and its timely disassembly is needed for developmental progression. Nuclear F-actin is a hallmark of totipotent zygotic PN, and the temporal regulation of its polymerized state is necessary for normal embryonic development.
  • Shota Yamazaki; Christian Gerhold; Koji Yamamoto; Yuya Ueno; Robert Grosse; Kei Miyamoto; Masahiko Harata
    Cells 9 (3) 2020/03 [Refereed]
     
    The crosstalk between actin and actin-related proteins (Arps), namely Arp2 and Arp3, plays a central role in facilitating actin polymerization in the cytoplasm and also in the nucleus. Nuclear F-actin is required for transcriptional regulation, double-strand break repair, and nuclear organization. The formation of nuclear F-actin is highly dynamic, suggesting the involvement of positive and negative regulators for nuclear actin polymerization. While actin assembly factors for nuclear F-actin have been recently described, information about inhibitory factors is still limited. The actin-related protein Arp4 which is predominantly localized in the nucleus, has been previously identified as an integral subunit of multiple chromatin modulation complexes, where it forms a heterodimer with monomeric actin. Therefore, we tested whether Arp4 functions as a suppressor of nuclear F-actin formation. The knockdown of Arp4 (Arp4 KD) led to an increase in nuclear F-actin formation in NIH3T3 cells, and purified Arp4 potently inhibited F-actin formation in mouse nuclei transplanted into Xenopus laevis oocytes. Consistently, Arp4 KD facilitated F-actin-inducible gene expression (e.g., OCT4) and DNA damage repair. Our results suggest that Arp4 has a critical role in the formation and functions of nuclear F-actin.
  • Higuchi C; Yamamoto M; Shin SW; Miyamoto K; Matsumoto K
    Biology open The Company of Biologists 8 (10) bio048652 - bio048652 2019/10 [Refereed]
  • Almonacid M; Al Jord A; El-Hayek S; Othmani A; Coulpier F; Lemoine S; Miyamoto K; Grosse R; Klein C; Piolot T; Mailly P; Voituriez R; Genovesio A; Verlhac MH
    Developmental cell 1534-5807 2019/10 [Refereed]
  • 2万8千年前のケナガマンモス組織から得られたタンパク質の翻訳後修飾の解析
    西端 智也; 永井 宏平; 山縣 一夫; 宮本 裕史; 安齋 政幸; 加藤 博己; 宮本 圭; 東 里香; Kolodeznikov Igor I.; Protopopov Albert V.; Plotnikov Valerii V.; 細井 美彦; 三谷 匡; 松本 和也; 入谷 明
    電気泳動 日本電気泳動学会 63 (Suppl.) 195 - 195 2189-2628 2019/07
  • 2万8千年前のケナガマンモスの筋肉組織と骨髄組織のプロテオーム解析
    永井 宏平; 宮本 裕史; 安齋 政幸; 東 里香; 西端 智也; 山縣 一夫; 加藤 博己; 宮本 圭; Kolodeznikov Igor I.; Protopopov Albert V.; Plotnikov Valerii V.; 細井 美彦; 三谷 匡; 松本 和也; 入谷 明
    電気泳動 日本電気泳動学会 63 (Suppl.) 196 - 196 2189-2628 2019/07
  • Yamagata K; Nagai K; Miyamoto H; Anzai M; Kato H; Miyamoto K; Kurosaka S; Azuma R; Kolodeznikov II; Protopopov AV; Plotnikov VV; Kobayashi H; Kawahara-Miki R; Kono T; Uchida M; Shibata Y; Handa T; Kimura H; Hosoi Y; Mitani T; Matsumoto K; Iritani A
    Scientific reports 9 (1) 4050 - 4050 2019/03 [Refereed]
     
    The 28,000-year-old remains of a woolly mammoth, named 'Yuka', were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from the remains and visualised their dynamics in living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated the presence of nuclear components in the remains. Nucleus-like structures found in the tissue homogenate were histone- and lamin-positive by immunostaining. In the reconstructed oocytes, the mammoth nuclei showed the spindle assembly, histone incorporation and partial nuclear formation; however, the full activation of nuclei for cleavage was not confirmed. DNA damage levels, which varied among the nuclei, were comparable to those of frozen-thawed mouse sperm and were reduced in some reconstructed oocytes. Our work provides a platform to evaluate the biological activities of nuclei in extinct animal species.
  • Miyamoto K
    The Journal of reproduction and development 65 (3) 203 - 208 0916-8818 2019/02 [Refereed][Invited]
  • 受精卵およびクローン胚におけるエピジェネティックリプログラミング
    奥野智美; 松本和也; 宮本 圭
    日本胚移植学雑誌 40 (3) 117 - 122 2018/09 [Refereed][Invited]
  • Miyamoto K; Nguyen KT; Allen GE; Jullien J; Kumar D; Otani T; Bradshaw CR; Livesey FJ; Kellis M; Gurdon JB
    Cell reports Elsevier {BV} 24 (2) 304 - 311 2018/07 [Refereed]
  • Rika Azuma; Kei Miyamoto; Mami Oikawa; Masayasu Yamada; Masayuki Anzai
    Journal of visualized experiments : JoVE (134) e57036  2018/04 [Refereed][Invited]
     
    Somatic cell nuclear transfer (SCNT) provides a unique opportunity to directly produce a cloned animal from a donor cell, and it requires the use of skillful techniques. Additionally, the efficiencies of cloning have remained low since the successful production of cloned animals, especially mice. There have been many attempts to improve the cloning efficiency, and trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to enhance the efficiency of cloning. Here, we report a dramatically improved cloning method in mice. This somatic cell nuclear transfer method involves usage of Hemagglutinating virus of Japan Envelope (HVJ-E), which enables easy manipulation. Moreover, the treatment using two small molecules, TSA and vitamin C (VC), with deionized bovine serum albumin (dBSA), is highly effective for embryonic development. This approach requires neither additional injection nor genetic manipulation, and thus presents a simple, suitable method for practical use. This method could become a technically feasible approach for researchers to produce genetically modified animals from cultured cells. Furthermore, it might be a useful way for the rescue of endangered animals via cloning.
  • Takashi Ikeda; Takafusa Hikichi; Hisashi Miura; Hirofumi Shibata; Kanae Mitsunaga; Yosuke Yamada; Knut Woltjen; Kei Miyamoto; Ichiro Hiratani; Yasuhiro Yamada; Akitsu Hotta; Takuya Yamamoto; Keisuke Okita; Shinji Masui
    Nature communications 9 (1) 1387 - 1387 2018/04 [Refereed]
     
    Multicellular organisms consist of multiple cell types. The identity of these cells is primarily maintained by cell-type-specific gene expression programs; however, mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease β-actin monomer quantity result in the nuclear accumulation of Mkl1 and the activation of Srf, which downregulate cell-type-specific genes and alter the epigenetics of regulatory regions and chromatin organization. Mice overexpressing Srf exhibit various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
  • 黒毛和種去勢牛の脂肪交雑を生体評価するバイオマーカー候補タンパク質の血清プロテオーム 解析による探索
    池上春香; 松橋珠子; 永井宏平; 宮本圭; 大林賢伍; 坂口慎一; 松本和也
    関西畜産学会報 (第175号) 2018/03 [Refereed]
  • Kohtaro Morita; Mikiko Tokoro; Yuki Hatanaka; Chika Higuchi; Haruka Ikegami; Kouhei Nagai; Masayuki Anzai; Hiromi Kato; Tasuku Mitani; Yoshitomo Taguchi; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    Journal of Reproduction and Development The Japanese Society of Animal Reproduction (JSAR) 64 (2) 161 - 171 1348-4400 2018 [Refereed]
     
    Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
  • 初期胚特異的レトロトランスポゾンMERVLの活性化に伴う核内変化
    奥野 智美; 山口 壮輝; 濱田 歩花; 竹林 真理愛; 守田 昂太郎; 樋口 智香; 神谷 拓磨; 安齋 政幸; 山縣 一夫; 細井 美彦; Jerome Jullien; 松本 和也; 宮本 圭
    生命科学系学会合同年次大会 生命科学系学会合同年次大会運営事務局 2017年度 [2P - 0708] 2017/12
  • Higuchi C; Shimizu N; Shin SW; Morita K; Nagai K; Anzai M; Kato H; Mitani T; Yamagata K; Hosoi Y; Miyamoto K; Matsumoto K
    The Journal of reproduction and development Japanese Society of Animal Reproduction 64 (1) 65 - 74 0916-8818 2017/12 [Refereed]
  • Christian Baarlink; Matthias Plessner; Alice Sherrard; Kohtaro Morita; Shinji Misu; David Virant; Eva-Maria Kleinschnitz; Robert Harniman; Dominic Alibhai; Stefan Baumeister; Kei Miyamoto; Ulrike Endesfelder; Abderrahmane Kaidi; Robert Grosse
    NATURE CELL BIOLOGY NATURE PUBLISHING GROUP 19 (12) 1389 - + 1465-7392 2017/12 [Refereed]
     
    Re-establishment of nuclear structure and chromatin organization after cell division is integral for genome regulation or development and is frequently altered during cancer progression. The mechanisms underlying chromatin expansion in daughter cells remain largely unclear. Here, we describe the transient formation of nuclear actin filaments (F-actin) during mitotic exit. These nuclear F-actin structures assemble in daughter cell nuclei and undergo dynamic reorganization to promote nuclear protrusions and volume expansion throughout early G1 of the cell cycle. Specific inhibition of this nuclear F-actin assembly impaired nuclear expansion and chromatin decondensation after mitosis and during early mouse embryonic development. Biochemical screening for mitotic nuclear F-actin interactors identified the actin-disassembling factor cofilin-1. Optogenetic regulation of cofilin-1 revealed its critical role for controlling timing, turnover and dynamics of F-actin assembly inside daughter cell nuclei. Our findings identify a cell-cycle-specific and spatiotemporally controlled form of nuclear F-actin that reorganizes the mammalian nucleus after mitosis.
  • Kei Miyamoto; Yosuke Tajima; Koki Yoshida; Mami Oikawa; Rika Azuma; George E. Allen; Tomomi Tsujikawa; Tomomasa Tsukaguchi; Charles R. Bradshaw; Jerome Jullien; Kazuo Yamagata; Kazuya Matsumoto; Masayuki Anzai; Hiroshi Imai; John B. Gurdon; Masayasu Yamada
    BIOLOGY OPEN COMPANY OF BIOLOGISTS LTD 6 (4) 415 - 424 2046-6390 2017/04 [Refereed]
     
    Animal cloning has been achieved in many species by transplanting differentiated cell nuclei to unfertilized oocytes. However, the low efficiencies of cloning have remained an unresolved issue. Here we find that the combination of two small molecules, trichostatin A (TSA) and vitamin C (VC), under culture condition with bovine serum albumin deionized by ion-exchange resins, dramatically improves the cloning efficiency in mice and 15% of cloned embryos develop to term by means of somatic cell nuclear transfer (SCNT). The improvement was not observed by adding the non-treated, rather than deionized, bovine serum. RNA-seq analyses of SCNT embryos at the two-cell stage revealed that the treatment with TSA and VC resulted in the upregulated expression of previously identified reprogramming-resistant genes. Moreover, the expression of early-embryo-specific retroelements was upregulated by the TSA and VC treatment. The enhanced gene expression was relevant to the VC-mediated reduction of histone H3 lysine 9 methylation in SCNT embryos. Our study thus shows a simply applicable method to greatly improve mouse cloning efficiency, and furthers our understanding of how somatic nuclei acquire totipotency.
  • Shinji Misu; Marina Takebayashi; Kei Miyamoto
    FRONTIERS IN GENETICS FRONTIERS MEDIA SA 8 27  1664-8021 2017/03 [Refereed][Invited]
     
    Actin is a highly abundant protein in eukaryotic cells and dynamically changes its polymerized states with the help of actin-binding proteins. Its critical function as a constituent of cytoskeleton has been well-documented. Growing evidence demonstrates that actin is also present in nuclei, referred to as nuclear actin, and is involved in a number of nuclear processes, including transcriptional regulation and chromatin remodeling. The contribution of nuclear actin to transcriptional regulation can be explained by its direct interaction with transcription machineries and chromatin remodeling factors and by controlling the activities of transcription factors. In both cases, polymerized states of nuclear actin affect the transcriptional outcome. Nuclear actin also plays an important role in activating strongly silenced genes in somatic cells for transcriptional reprogramming. When these nuclear functions of actin are considered, it is plausible to speculate that nuclear actin is also implicated in embryonic development, in which numerous genes need to be activated in a well-coordinated manner. In this review, we especially focus on nuclear actin's roles in transcriptional activation, reprogramming and development, including stem cell differentiation and we discuss how nuclear actin can be an important player in development and cell differentiation.
  • Jerome Jullien; Munender Vodnala; Vincent Pasque; Mami Oikawa; Kei Miyamoto; George Allen; Sarah Anne David; Vincent Brochard; Stan Wang; Charles Bradshaw; Haruhiko Koseki; Vittorio Sartorelli; Nathalie Beaujean; John Gurdon
    MOLECULAR CELL CELL PRESS 65 (5) 873 - + 1097-2765 2017/03 [Refereed]
     
    Understanding the mechanism of resistance of genes to reactivation will help improve the success of nuclear reprogramming. Using mouse embryonic fibroblast nuclei with normal or reduced DNA methylation in combination with chromatin modifiers able to erase H3K9me3, H3K27me3, and H2AK119ub1 from transplanted nuclei, we reveal the basis for resistance of genes to transcriptional reprogramming by oocyte factors. A majority of genes is affected by more than one type of treatment, suggesting that resistance can require repression through multiple epigenetic mechanisms. We classify resistant genes according to their sensitivity to 11 chromatin modifier combinations, revealing the existence of synergistic as well as adverse effects of chromatin modifiers on removal of resistance. We further demonstrate that the chromatin modifier USP21 reduces resistance through its H2AK119 deubiquitylation activity. Finally, we provide evidence that H2A ubiquitylation also contributes to resistance to transcriptional reprogramming in mouse nuclear transfer embryos.
  • Marta Teperek; Angela Simeone; Vincent Gaggioli; Kei Miyamoto; George E. Allen; Serap Erkek; Taejoon Kwon; Edward M. Marcotte; Philip Zegerman; Charles R. Bradshaw; Antoine H. F. M. Peters; John B. Gurdon; Jerome Jullien
    GENOME RESEARCH COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT 26 (8) 1034 - 1046 1088-9051 2016/08 [Refereed]
     
    For a long time, it has been assumed that the only role of sperm at fertilization is to introduce the male genome into the egg. Recently, ideas have emerged that the epigenetic state of the sperm nucleus could influence transcription in the embryo. However, conflicting reports have challenged the existence of epigenetic marks on sperm genes, and there are no functional tests supporting the role of sperm epigenetic marking on embryonic gene expression. Here, we show that sperm is epigenetically programmed to regulate embryonic gene expression. By comparing the development of sperm-and spermatid-derived frog embryos, we show that the programming of sperm for successful development relates to its ability to regulate transcription of a set of developmentally important genes. During spermatid maturation into sperm, these genes lose H3K4me2/3 and retain H3K27me3 marks. Experimental removal of these epigenetic marks at fertilization de-regulates gene expression in the resulting embryos in a paternal chromatin-dependent manner. This demonstrates that epigenetic instructions delivered by the sperm at fertilization are required for correct regulation of gene expression in the future embryos. The epigenetic mechanisms of developmental programming revealed here are likely to relate to the mechanisms involved in transgenerational transmission of acquired traits. Understanding how parental experience can influence development of the progeny has broad potential for improving human health.
  • A new era of human assisted reproductive technology
    K. Miyamoto
    Journal of Mammalian Ova Research 33 (2) 77 - 77 2016 [Refereed][Invited]
  • Kei Miyamoto; Ken-ichi T. Suzuki; Miyuki Suzuki; Yuto Sakane; Tetsushi Sakuma; Sarah Herberg; Angela Simeone; David Simpson; Jerome Jullien; Takashi Yamamoto; J. B. Gurdon
    PLOS ONE PUBLIC LIBRARY SCIENCE 10 (11) e0142946  1932-6203 2015/11 [Refereed]
     
    Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock-out in founder embryos (F0) needs to be explored in order to judge phenotypes in the F0 generation. Here, we injected modified highly active transcription activator-like effector nuclease (TALEN) mRNA to oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation and intracytoplasmic sperm injection, to achieve a full knock-out in F0 embryos. Unlike conventional injection methods to fertilized embryos, the injection of TALEN mRNA into GV oocytes allows expression of nucleases before fertilization, enabling them to work from an earlier stage. Using this procedure, most of developed embryos showed full knock-out phenotypes of the pigmentation gene tyrosinase and/or embryonic lethal gene pax6 in the founder generation. In addition, our method permitted a large 1 kb deletion. Thus, we describe nearly complete gene knock-out phenotypes in Xenopus laevis F0 embryos. The presented method will help to accelerate the production of knock-out frogs since we can bypass an extra generation of about 1 year in Xenopus laevis. Meantime, our method provides a unique opportunity to rapidly test the developmental effects of disrupting those genes that do not permit growth to an adult able to reproduce. In addition, the protocol shown here is considerably less invasive than the previously used host transfer since our protocol does not require surgery. The experimental scheme presented is potentially applicable to other organisms such as mammals and fish to resolve common issues of mosaicism in founders.
  • Sarah Herberg; Angela Simeone; Mami Oikawa; Jerome Jullien; Charles R. Bradshaw; Marta Teperek; John Gurdon; Kei Miyamoto
    SCIENTIFIC REPORTS NATURE PUBLISHING GROUP 5 14236  2045-2322 2015/09 [Refereed]
     
    Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their developmentally regulated expression has been shown, the mechanisms controlling retrotransposon expression in early embryos are still not well understood. Here, we observe a dynamic expression pattern of retrotransposons with three out of ten examined retrotransposons (1a11, lambda-olt 2-1 and xretpos( L)) being transcribed solely during early embryonic development. We also identified a transcript that contains the long terminal repeat (LTR) of lambda-olt 2-1 and shows a similar expression pattern to lambda-olt 2-1 in early Xenopus embryos. All three retrotransposons are transcribed by RNA polymerase II. Although their expression levels decline during development, the LTRs are marked by histone H3 lysine 4 trimethylation. Furthermore, retrotransposons, especially lambda-olt 2-1, are enriched with histone H3 lysine 9 trimethylation (H3K9me3) when their expression is repressed. Overexpression of lysine-specific demethylase 4d removes H3K9me3 marks from Xenopus embryos and inhibits the repression of lambda-olt 2-1 after gastrulation. Thus, our study shows that H3K9me3 is important for silencing the developmentally regulated retrotransposon in Xenopus laevis.
  • 塚口 智将; 守田 昴太郎; 宮本 圭; 松本和也
    Memoirs of Institute of Advanced Technology, Kinki University 近畿大学先端技術総合研究所 6号 (20) 1 - 8 1346-8693 2015/03 [Refereed]
     
    [要旨] 哺乳類の初期胚では, 分化全能性を獲得するために, 受精直後に終末分化した精子と卵子のエピゲノムの情報がリセットされる. この現象では, 遺伝子の転写制御に関与するDNA やヒストンのメチル化及びアセチル化修飾がダイナミックに変化することが知られている. 受精後, 遺伝子の転写抑制に関わるDNAのメチル化修飾は, 酵素及びDNA 複製によって取り除かれ, その後再び, DNA メチル基転移酵素(DNAmethyltransferases, DNMTs)が働くことにより, 特定の遺伝子の転写制御が行われる. ヒストンにおいては, histone methyltransferase(HMT)によるメチル化, histone acetyltransferase(HAT)によるアセチル化などの修飾がクロマチン構造の変化を誘起させ, 転写の活性あるいは抑制に関わっている. 以上のように遺伝子の発現は, DNA やヒストン修飾の相互の働きかけによって制御されていると考えられる. したがって, 初期胚で起こるDNA 及びヒストン修飾の機構やそれらの修飾の持つ役割を知ることで, エピジェネティックリプログラミングの詳細な分子機構を深く理解することができる. さらに, それらの知見をiPS細胞作製効率の向上や分化誘導の改善に応用させることで, 再生医療や創薬分野への貢献が期待される. そこで本稿では, 哺乳類の初期胚において発生に必要な遺伝子の転写制御に関与しているDNA 及びヒストンのメチル化修飾について概説する. [Abstract] In mammalian early embryo, epigenome information of sperm and oocytes is reset to acquire the developmental totipotency. This phenomenon is accompanied by the dynamic changes in DNA and histone modifications. DNA methylation involved in transcriptional repression is removed by the enzymes or DNA replication after fertilization. The genome is remethylated by DNA methyltransferases(DNMTs) and the specific set of genes is transcriptionally repressed. Histone modifications such as methylation by histone methyltransferases(HMTs) and acetylation by histone acetyltransferases(HATs) also induce changes in chromatin structure and are involved in transcriptional regulation. Therefore, gene expression regulation seems to be achieved by the combination of DNA and histone modifications. Consequently, understanding mechanisms and roles of DNA and histone modifications can be a clue to understand detailed mechanism of epigenetic reprogramming. Moreover, the basic knowledge about epigenetic reprogramming to improve iPS cell conversion and induction of differentiation would contribute to the fields of regenerative medicine and innovative drug development. Here, we will review DNA and histone methylation related totranscriptional regulation of genes necessary for development in mammalian early embryo.近畿大学先端技術総合研究所紀要編集委員会
  • Kei Miyamoto; David Simpson; John B. Gurdon
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS JOURNAL OF VISUALIZED EXPERIMENTS (96) e52496  1940-087X 2015/02 [Refereed][Invited]
     
    Amphibian eggs have been widely used to study embryonic development. Early embryonic development is driven by maternally stored factors accumulated during oogenesis. In order to study roles of such maternal factors in early embryonic development, it is desirable to manipulate their functions from the very beginning of embryonic development. Conventional ways of gene interference are achieved by injection of antisense oligonucleotides (oligos) or mRNA into fertilized eggs, enabling under-or over-expression of specific proteins, respectively. However, these methods normally require more than several hours until protein expression is affected, and, hence, the interference of gene functions is not effective during early embryonic stages. Here, we introduce an experimental system in which expression levels of maternal proteins can be altered before fertilization. Xenopus laevis oocytes obtained from ovaries are defolliculated by incubating with enzymes. Antisense oligos or mRNAs are injected into defolliculated oocytes at the germinal vesicle (GV) stage. These oocytes are in vitro matured to eggs at the metaphase II (MII) stage, followed by intracytoplasmic sperm injection (ICSI). By this way, up to 10% of ICSI embryos can reach the swimming tadpole stage, thus allowing functional tests of specific gene knockdown or overexpression. This approach can be a useful way to study roles of maternally stored factors in early embryonic development.
  • Marta Teperek; Kei Miyamoto; Angela Simeone; Renata Feret; Michael J. Deery; John B. Gurdon; Jerome Jullien
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES MDPI AG 15 (9) 16719 - 16740 1422-0067 2014/09 [Refereed][Invited]
     
    Spermatozoa are more efficient at supporting normal embryonic development than spermatids, their immature, immediate precursors. This suggests that the sperm acquires the ability to support embryonic development during spermiogenesis (spermatid to sperm maturation). Here, using Xenopus laevis as a model organism, we performed 2-D Fluorescence Difference Gel Electrophoresis (2D-DIGE) and mass spectrometry analysis of differentially expressed proteins between sperm and spermatids in order to identify factors that could be responsible for the efficiency of the sperm to support embryonic development. Furthermore, benefiting from the availability of egg extracts in Xenopus, we also tested whether the chromatin of sperm could attract different egg factors compared to the chromatin of spermatids. Our analysis identified: (1) several proteins which were present exclusively in sperm; but not in spermatid nuclei and (2) numerous egg proteins binding to the sperm (but not to the spermatid chromatin) after incubation in egg extracts. Amongst these factors we identified many chromatin-associated proteins and transcriptional repressors. Presence of transcriptional repressors binding specifically to sperm chromatin could suggest its preparation for the early embryonic cell cycles, during which no transcription is observed and suggests that sperm chromatin has a unique protein composition, which facilitates the recruitment of egg chromatin remodelling factors. It is therefore likely that the acquisition of these sperm-specific factors during spermiogenesis makes the sperm chromatin suitable to interact with the maternal factors and, as a consequence, to support efficient embryonic development.
  • Jerome Jullien; Kei Miyamoto; Vincent Pasque; George E. Allen; Charles R. Bradshaw; Nigel J. Garrett; Richard P. Halley-Stott; Hiroshi Kimura; Keita Ohsumi; John B. Gurdon
    MOLECULAR CELL CELL PRESS 55 (4) 524 - 536 1097-2765 2014/08 [Refereed]
     
    Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a selective switch in transcription toward an oocyte rather than pluripotent type, without requiring new protein synthesis. Time-course analyses at the single-nucleus level show that transcriptional reprogramming is induced in most transplanted nuclei in a highly hierarchical manner. We demonstrate that an extensive exchange of somatic-for oocyte-specific factors mediates reprogramming and leads to robust oocyte RNA polymerase II binding and phosphorylation on transplanted chromatin. Moreover, genome-wide binding of oocyte-specific linker histone B4 supports its role in transcriptional reprogramming. Thus, our study reveals the rapid, abundant, and stepwise loading of oocyte-specific factors onto somatic chromatin as important determinants for successful reprogramming.
  • Kei Miyamoto
    Journal of Mammalian Ova Research JAPANESE SOCIETY OF OVA RESEARCH 30 (3) 68 - 78 1341-7738 2013/10 [Refereed][Invited]
     
    When a somatic cell nucleus is transplanted into an egg or an oocyte, the transplanted nucleus can be reprogrammed to support early embryonic development so that the reconstructed embryo gives rise to a cloned animal. Nuclear reprogramming of somatic nuclei is induced by maternal components stored in eggs and oocytes. These endogenous reprogramming factors and mechanisms have been explored for decades in mammals and amphibia. There are several ways of investigating reprogramming mechanisms, including nuclear transfer to eggs/oocytes and incubation in egg/oocyte extracts. In this review I describe the type of reprogramming events induced in each system and what factors in eggs and oocytes are responsible for these. Based on our current knowledge, I propose a model for the early phase of nuclear reprogramming in eggs and oocytes.
  • Miyamoto K; Gurdon JB
    Cellular and molecular life sciences : CMLS 18 70 (18) 3289 - 3302 1420-682X 2013/09 [Refereed][Invited]
  • Kei Miyamoto; Marta Teperek; Kosuke Yusa; George E. Allen; Charles R. Bradshaw; J. B. Gurdon
    SCIENCE AMER ASSOC ADVANCEMENT SCIENCE 341 (6149) 1002 - 1005 0036-8075 2013/08 [Refereed]
     
    Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization and in somatic nuclei after somatic cell nuclear transfer. This ability of eggs and oocytes is essential for normal development. Nuclear actin and actin-binding proteins have been shown to contribute to transcription, although their mode of action is elusive. Here, we find that Xenopus Wave1, previously characterized as a protein involved in actin cytoskeleton organization, is present in the oocyte nucleus and is required for efficient transcriptional reprogramming. Moreover, Wave1 knockdown in embryos results in abnormal development and defective hox gene activation. Nuclear Wave1 binds by its WHD domain to active transcription components, and this binding contributes to the action of RNA polymerase II. We identify Wave1 as a maternal reprogramming factor that also has a necessary role in gene activation in development.
  • Teperek M; Miyamoto K
    Reproductive medicine and biology 12 (4) 133 - 149 1445-5781 2013/06 [Refereed][Invited]
  • Patrick Narbonne; Kei Miyamoto; J. B. Gurdon
    CURRENT OPINION IN GENETICS & DEVELOPMENT CURRENT BIOLOGY LTD 22 (5) 450 - 458 0959-437X 2012/10 [Refereed][Invited]
     
    Nuclear transfer (NT) remains the most effective method to reprogram somatic cells to totipotency. Somatic cell nuclear transfer (SCNT) efficiency however remains low, but recurrent problems occurring in partially reprogrammed cloned embryos have recently been identified and some remedied. In particular, the trophectoderm has been identified as a lineage whose reprogramming success has a large influence on SCNT embryo development. Several interspecific hybrid and cybrid reprogramming systems have been developed as they offer various technical advantages and potential applications, and together with SCNT, they have led to the identification of a series of reprogramming events and responsible reprogramming factors. Interspecific incompatibilities hinder full exploitation of cross-species reprogramming systems, yet recent findings suggest that these may not constitute insurmountable obstacles.
  • Vincent Pasque; Jerome Jullien; Kei Miyamoto; Richard P. Halley-Stott; J. B. Gurdon
    TRENDS IN GENETICS ELSEVIER SCIENCE LONDON 27 (12) 516 - 525 0168-9525 2011/12 [Refereed][Invited]
     
    Patient-specific somatic cell reprogramming is likely to have a large impact on medicine by providing a source of cells for disease modelling and regenerative medicine. Several strategies can be used to reprogram cells, yet they are generally characterised by a low reprogramming efficiency, reflecting the remarkable stability of the differentiated state. Transcription factors, chromatin modifications, and noncoding RNAs can increase the efficiency of reprogramming. However, the success of nuclear reprogramming is limited by epigenetic mechanisms that stabilise the state of gene expression in somatic cells and thereby resist efficient reprogramming. We review here the factors that influence reprogramming efficiency, especially those that restrict the natural reprogramming mechanisms of eggs and oocytes. We see this as a step towards understanding the mechanisms by which nuclear reprogramming takes place.
  • Kei Miyamoto; Vincent Pasque; John B. Gurdon
    CELL CYCLE LANDES BIOSCIENCE 10 (18) 3040 - 3041 1538-4101 2011/09 [Refereed][Invited]
  • Miyamoto K; Gurdon JB
    Communicative & integrative biology 5 4 (5) 582 - 583 2011/09 [Refereed][Invited]
  • Jerome Jullien; Vincent Pasque; Richard P. Halley-Stott; Kei Miyamoto; J. B. Gurdon
    NATURE REVIEWS MOLECULAR CELL BIOLOGY NATURE PUBLISHING GROUP 12 (7) 453 - 459 1471-0072 2011/07 [Refereed][Invited]
     
    Differentiated cells can be experimentally reprogrammed back to pluripotency by nuclear transfer, cell fusion or induced pluripotent stem cell technology. Nuclear transfer and cell fusion can lead to efficient reprogramming of gene expression. The egg and oocyte reprogramming process includes the exchange of somatic proteins for oocyte proteins, the post-translational modification of histones and the demethylation of DNA. These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes.
  • Kei Miyamoto; Vincent Pasque; Jerome Jullien; John B. Gurdon
    GENES & DEVELOPMENT COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT 25 (9) 946 - 958 0890-9369 2011/05 [Refereed]
     
    Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei. To explore the factors and mechanisms involved, we focused on nuclear actin, an especially abundant component of the oocyte's nucleus (the germinal vesicle). The existence and significance of nuclear actin has long been debated. Here, we found that nuclear actin polymerization plays an essential part in the transcriptional reactivation of the pluripotency gene Oct4 (also known as Pou5f1). We also found that an actin signaling protein, Toca-1, enhances Oct4 reactivation by regulating nuclear actin polymerization. Toca-1 overexpression has an effect on the chromatin state of transplanted nuclei, including the enhanced binding of nuclear actin to gene regulatory regions. This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner.
  • Kei Miyamoto; Kouhei Nagai; Naoya Kitamura; Tomoaki Nishikawa; Haruka Ikegami; Nguyen T. Binh; Satoshi Tsukamoto; Mai Matsumoto; Tomoyuki Tsukiyama; Naojiro Minami; Masayasu Yamada; Hiroyoshi Ariga; Masashi Miyake; Tatsuo Kawarasaki; Kazuya Matsumoto; Hiroshi Imai
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA NATL ACAD SCIENCES 108 (17) 7040 - 7045 0027-8424 2011/04 [Refereed]
     
    Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine oocyte extracts in which some reprogramming events are recapitulated. After incubating somatic nuclei in oocyte extracts from the metaphase II stage, the oocyte proteins that were specifically and abundantly incorporated into the nuclei were identified by mass spectrometry. Among 25 identified proteins, we especially focused on a multifunctional protein, DJ-1. DJ-1 is present at a high concentration in oocytes from the germinal vesicle stage until embryos at the four-cell stage. Inhibition of DJ-1 function compromises the development of nuclear transfer embryos but not that of fertilized embryos. Microarray analysis of nuclear transfer embryos in which DJ-1 function is inhibited shows perturbed expression of P53 pathway components. In addition, embryonic arrest of nuclear transfer embryos injected with anti-DJ-1 antibody is rescued by P53 inhibition. We conclude that DJ-1 is an oocyte factor that is required for development of nuclear transfer embryos. This study presents a means for identifying natural reprogramming factors in mammalian oocytes and a unique insight into the mechanisms underlying reprogramming by nuclear transfer.
  • Pasque V; Miyamoto K; Gurdon JB
    Cold Spring Harb Symp Quant Biol 75 189 - 200 2010/11 [Refereed][Invited]
  • R. P. Halley-Stott; V. Pasque; C. Astrand; K. Miyamoto; I. Simeoni; J. Jullien; J. B. Gurdon
    METHODS ACADEMIC PRESS INC ELSEVIER SCIENCE 51 (1) 56 - 65 1046-2023 2010/05 [Refereed][Invited]
     
    Full-grown Xenopus oocytes in first meiotic prophase contain an immensely enlarged nucleus, the Germinal Vesicle (GV), that can be injected with several hundred somatic cell nuclei. When the nuclei of mammalian somatic cells or cultured cell lines are injected into a GV, a wide range of genes that are not transcribed in the donor cells, including pluripotency genes, start to be transcriptionally activated, and synthesize primary transcripts continuously for several days. Because of the large size and abundance of Xenopus laevis oocytes, this experimental system offers an opportunity to understand the mechanisms by which somatic cell nuclei can be reprogrammed to transcribe genes characteristic of oocytes and early embryos. The use of mammalian nuclei ensures that there is no background of endogenous maternal transcripts of the kind that are induced. The induced gene transcription takes place in the absence of cell division or DNA synthesis and does not require protein synthesis. Here we summarize new as well as established results that characterize this experimental system. In particular, we describe optimal conditions for transplanting somatic nuclei to oocytes and for the efficient activation of transcription by transplanted nuclei. We make a quantitative determination of transcript numbers for pluripotency and housekeeping genes, comparing cultured somatic cell nuclei with those of embryonic stem cells. Surprisingly we find that the transcriptional activation of somatic nuclei differs substantially from one donor cell-type to another and in respect of different pluripotency genes. We also determine the efficiency of an injected mRNA translation into protein. (C) 2010 Elsevier Inc. All rights reserved.
  • Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
    BIOLOGY OF REPRODUCTION SOC STUDY REPRODUCTION 80 (5) 935 - 943 0006-3363 2009/05 [Refereed]
     
    Nuclear transfer has been regarded as the only reliable tool for studying nuclear reprogramming of mammalian somatic cells by oocytes. However, nuclear transfer is not well suited for biochemical analyses of the molecular mechanisms of reprogramming. A cell-free system from oocytes is an attractive alternative way to mimic reprogramming in vitro, since a large number of cells can be treated and analyzed. Nevertheless, a cell-free system using oocytes has not been developed in mammals. Here, cell extracts from porcine oocytes were prepared and their ability to induce nuclear reprogramming was evaluated. Extracts from metaphase II (MII) oocytes erased the machinery for regulating gene expression in reversibly permeabilized somatic cells. For example, the extracts caused histone deacetylation and the disappearance of TATA box-binding protein from the nuclei. However, MII-extract-treated cells did not show any obvious changes after cell culture. In contrast, extracts from germinal vesicle (GV) oocytes activated pluripotent marker genes, especially NANOG, and induced partial dedifferentiation after cell culture. The activation of pluripotent marker genes by GV extracts was associated with histone acetylation that was induced during extract treatment. These results indicate that GV- and MII-oocyte extracts have different roles on nuclear reprogramming. Furthermore, both oocyte extracts induced site-specific demethylation in the upstream region of NANOG. These results indicate that cell-free extracts derived from GV- and MII-oocytes could be useful for studying the mechanisms involved in nuclear reprogramming.
  • Kei Miyamoto; Teruyoshi Yamashita; Tomoyuki Tsukiyama; Naoya Kitamura; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
    CLONING AND STEM CELLS MARY ANN LIEBERT, INC 10 (4) 535 - 542 1536-2302 2008/12 [Refereed]
     
    Plasma membranes can be reversibly permeabilized by Streptolysin O. The permeabilized cells can be reprogrammed and partially dedifferentiated in the cell-free system from egg extracts. However, the permeabilizing activity of Streptolysin 0 is not stable, and therefore it is difficult to control its activity. An alternative method for reversible permeabilization is useful for establishing a cell-free system. Here, we used a nonionic detergent, digitonin, for permeabilization. A low concentration of digitonin induced reversible permeabilization of the plasma membrane in bovine, mouse, and porcine somatic cells. The permeabilized cells were treated with Xenoptis laevis egg extracts. The treated cells showed exchange of nuclear proteins from extracts such as incorporation of Xenopus-specific histone B4 and Lamin LIII into nuclei. After resealing of the membrane, the cells showed upregulation of OCT4, SOX2, and NANOG expression. Our results suggest that reversible permeabilization with digitonin can be used to induce nuclear reprogramming and to activate pluripotent genes by a cell-free system.
  • K. Miyamoto; T. Tsukiyama; N. Minami; M. Yamada; H. Imai
    REPRODUCTION IN DOMESTIC ANIMALS WILEY-BLACKWELL PUBLISHING, INC 43 194 - 194 0936-6768 2008/07 [Refereed]
  • Tomoyuki Tsukiyama; Kei Miyamoto; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
    BIOLOGY OF REPRODUCTION SOC STUDY REPRODUCTION 85 - 85 0006-3363 2008 [Refereed]
  • Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
    BIOLOGY OF REPRODUCTION SOC STUDY REPRODUCTION 55 - 55 0006-3363 2008 [Refereed]
  • Kei Miyamoto; Tadashi Furusawa; Mari Ohnuki; Sandeep Goel; Tomoyuki Tokunaga; Naojiro Minami; Masayasu Yamada; Keita Ohsumi; Hiroshi Imai
    MOLECULAR REPRODUCTION AND DEVELOPMENT WILEY-LISS 74 (10) 1268 - 1277 1040-452X 2007/10 [Refereed]
     
    It is known that differentiated cells can be reprogrammed to an undifferentiated state in oocyte cytoplasm after nuclear transfer. Recently, some reports suggested that Xenopus egg extracts have the ability to reprogram mammalian somatic cells. Reprogramming events of mammalian cells after Xenopus egg extract treatment and after cell culture of extract-treated cells have not been elucidated. In this experiment, we examined reprogramming events in reversibly permeabilized or nonpermeabilized porcine fibroblast cells after Xenopus egg extract treatment. The Xenopus egg-specific histone 134 was assembled on porcine chromatin and nuclear lamin LIII was incorporated into nuclei. Deacetylation of histone H3 at lysine 9 in extract-treated cells was detected in nonpermeabilized cells, suggesting that a part of reprogramming may be induced even in nonpermeabilized cells. Following culture of extract-treated cells, the cells began to express the pluripotent marker genes such as POU5F1 (OCT4) and SOX2 and to form colonies. Reactivation of the OCT4 gene in extract-treated cells was also confirmed in bovine fibroblasts transformed with an OCT4-EGFP construct. These results suggest that nuclei of mammalian cells can be partially reprogrammed to an embryonic state by Xenopus egg extracts and the remodeled cells partly dedifferentiate after cell culture. A system using egg extracts may be useful for understanding the mechanisms and processes of dedifferentiation and reprogramming of mammalian somatic cells after nuclear transfer.
  • Kei Miyamoto; Yoichiro Hoshino; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
    JOURNAL OF REPRODUCTION AND DEVELOPMENT SOCIETY REPRODUCTION & DEVELOPMENT-SRD 53 (2) 237 - 246 0916-8818 2007/04 [Refereed]
     
    The relationship between donor cell cycle and the developmental ability of somatic cell nuclear transfer (SCNT) embryos has not fully been elucidated. Donor cells that are usually prepared by serum starvation or confluent-cell culture for SCNT represent a heterogeneous population that includes mainly G0 phase cells, other cells in different phases of the cell cycle and apoptotic cells. In this study, we compared the developmental ability of porcine SCNT embryos reconstructed from G0 phase cells (GO-SCNT embryos) and strictly synchronized-G1 phase cells (G1-SCNT embryos), and examined the developmental rates and timing of first DNA synthesis. The G0 phase cells were synchronized by confluent culture, and the G1 phase cells were prepared from actively dividing M phase cells. The G1-SCNT embryos showed a significantly higher (P<0.05) developmental rate to the blastocyst stage per cleaved embryo (59%) than the G0-SCNT embryos (43%). Moreover, initiation of first DNA synthesis and cleavage occurred significantly earlier in the G1-SCNT embryos than in the G0-SCNT embryos. Delay of initiation of first DNA synthesis in the SCNT embryos by aphidicolin resulted in decreased developmental rates to the blastocyst stage without any effect on cleavage rates. Our data demonstrates that synchronized-G1 phase cells can be used as donor cells for SCNT embryos and that earlier initiation of first DNA synthesis may be important for subsequent development of SCNT embryos. The SCNT system using G1-synchronized cells, in terms of their highly uniform and viable cell states, can be useful for studying the reprogramming processes and embryonic development of SCNT embryos.
  • K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai
    REPRODUCTION FERTILITY AND DEVELOPMENT CSIRO PUBLISHING 19 (1) 150 - 150 1031-3613 2007 [Refereed]
  • 無細胞抽出系を用いた培養細胞のリプログラミングとその応用
    宮本 圭; 今井 裕
    日本胚移植学雑誌 28 (2) 81 - 87 2006/05 [Refereed]
  • Nuclear reprogramming of porcine fibroblast cells by Xenopus egg extracts.
    Miyamoto K; Nagao Y; Minami N; Yamada M; Ohsumi K; Imai H
    32nd International Embryo Transfer Society 2006 [Refereed]
  • 53 CELL CYCLE SYNCHRONIZATION OF DONOR CELLS AT G1 PHASE AND DEVELOPMENTAL ABILITY OF NUCLEAR TRANSFER EMBRYOS IN MINIATURE PIGS
    K Miyamoto; Y Hoshino; Y Nagao; N Minami; M Yamada; H Imai
    Reproduction, Fertility and Development 17 (2) 176 - 176 2004/12 [Refereed]

Books etc

  • 生命科学の未来を切り拓く マンモス復活プロジェクト,「Newton 近畿大学の理系学部と研究所を大特集 近畿大学 大解剖 vol.2 」
    松本和也; 三谷匡; 加藤博巳; 宮本裕史; 山縣一夫; 安齋政幸; 永井宏平; 宮本圭; 黒坂哲 ニュートンプレス 2021/07
  • 次世代の核移植技術の利用に向けて、Campus & Conference探訪記、「実験医学」
    宮本圭 羊土社 2016
  • 最先端と伝統が共存する場所、Lab Report、「実験医学」
    宮本圭 羊土社 2015
  • 母性因子Wave1は転写のリプログラミングと正常な胚発生に必要である、「実験医学」
    宮本圭 羊土社 2014
  • Biology & Pathology of the Oocyte
    宮本圭; John Gurdon (ContributorInsights into the amphibian egg to understand the mammalian oocyte.)Cambridge University Press 2013
  • ジョン・ガードン~リプログラミングの父「科学」
    宮本圭 岩波書店 2013/01
  • 卵細胞抽出液を用いた体細胞のリプログラミング誘導、「幹細胞の分化誘導と応用」
    宮本圭; 今井 裕 エヌ・ティー・エス出版 2009

Conference Activities & Talks

  • マウス初期胚発生における核構造の初期化  [Invited]
    宮本圭
    定量生物学の会 第十一回年会  2024/01
  • Nuclear reprogramming for animal reproduction and obtaining genomic information of various species  [Invited]
    Kei Miyamoto
    2nd ICFAS  2023/12
  • マウス2細胞期胚におけるLamina-associated domainsの同定  [Not invited]
    三島 花心; 坂上凛; 大里優介; 宮川靖基; 井橋俊哉; 鷹巣篤志; 西崎俊太朗; 井上明裕; 森龍之介; 門野莉紗; 佐藤英男; 清水奎伍; 松本和也; 前澤創; 宮本圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • マウス2細胞期胚におけるLamin B1の一過的な減少は胚発生において重要な役割を果たす  [Not invited]
    坂上凜; 田中真仁; 鷹巣篤志; 宮川靖基; 渡邊直子; 島本勇太; 宮本圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • 絶滅動物細胞核の転写リプログラミング誘導  [Not invited]
    門野 莉紗; クリストファー・ペンフォールド; 久住 和希; 井橋 俊哉; 鷹巣 篤志; 坂上凜; 井上 明裕; 西崎 俊太朗; 宮川 靖基; 森 龍之介; 三島 花心; 清水 奎伍; 佐藤 英男; 松本 和也; 安齋 政幸; 宮本 圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • 老化ドナー細胞が体細胞核移植の発生に与える影響  [Not invited]
    森龍之介; 西崎俊太郎; 井橋俊哉; 鷹巣篤志; 坂上凜; 井上明裕; 門野莉紗; 宮川靖基; 清水奎吾; 三島花心; 松本和也; 宮本圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • カエル卵抽出液を用いたマウス老化細胞へのpartial reprogramming誘導  [Not invited]
    西崎 俊太朗; 井橋俊哉; 森龍之介; 新冨圭史; 井上明裕; 鷹巣篤志; 坂上凛; 宮川靖基; 門野莉紗; 三島花心; 松本和也; 宮本圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • 母性転写産物を用いた哺乳類胚における新規胚発生予測法の確立に向けて  [Not invited]
    井上 明裕; Nicole Cheung; 山本真理; 塚口智将; 佐藤英男; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜子; 池田 智明; 山之内 忠幸; 松田 秀雄; 井橋 俊哉; 鷹巣 篤志; 坂上 凛; 西崎俊太朗; 宮川靖基; 森龍之介; 門野莉紗; 清水奎伍; 三島花心; 松本和也; 宮本圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • 母性転写産物の発現を指標にした胚の全能性評価法の開発  [Invited]
    宮本 圭
    全能性プログラム:デコーディングからデザインへ 第5回公開シンポジウム  2023/12
  • 野生動物細胞核の転写リプログラミング誘導  [Not invited]
    Risa Kadono; Christopher Penfold; Syunya Ihashi; Atsushi Takasu; Rin Sakanoue; Mana Hayashi; Akihiro Inoue; Shuntaro Nishizaki; Yasuki Miyagawa; Ryunosuke Mori; Kazuya Matsumoto; Masayuki Anzai; Kei Miyamoto
    第46回 日本分子生物学会年会  2023/12
  • Physical reprogramming of embryonic nuclei in mouse  [Invited]
    宮本圭
    第46回 日本分子生物学会年会  2023/11
  • 異種間核移植技術を用いた野生由来マウスES細胞の樹立
    渡邉 奈穂美; 廣瀬 美智子; 長谷川 歩未; 持田 慶司; 井橋 俊哉; 宮本 圭; 小倉 淳郎; 井上 貴美子
    第116回 日本繁殖生物学会大会  2023/09
  • 初期胚発生における核内Fアクチンの役割の解明  [Not invited]
    宮川 靖基; 坂上 凜; 眞銅 大暉; 坂本 裕子; 三島 花心; 井橋 俊哉; 鷹巣 篤志; 井上 明裕; 門野 莉紗; 西崎 俊太朗; 森 龍之介; 松本 和也; 宮本 圭
    第116回 日本繁殖生物学会大会  2023/09
  • 高品質受精卵選別法の確立に向けたバイオマーカー候補の探索  [Not invited]
    井上 明裕; 山本 真里; 井橋 俊哉; 鷹巣 篤志; 林 真那; 坂上 凜; 門野 莉紗; 西崎 俊太朗; 宮川 靖基; 森 龍之介; 松本 和也; 宮本 圭
    第116回 日本繁殖生物学会大会  2023/09
  • マウス2細胞期胚におけるラミンB1の一過的な減少は胚性ゲノム活性化に重要である
    坂上 凜; 田中 真仁; 鷹巣 篤志; 宮川 靖基; 渡辺 直子; 島本 勇太; 宮本 圭
    第116回 日本繁殖生物学会大会  2023/09
  • Reprogramming of nuclear structures for embryonic development in mouse.  [Invited]
    Miyamoto K
    DevStem Seminar (Nantes, France)  2023/08
  • identification of maternal transcripts that can the development potential of mammalian embryos for predicting full-term development  [Not invited]
    井上 明裕; Nicole Cheung; 山本真理; 塚口智将; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜子; 池田 智明; 山之内 忠幸; 松田 秀雄; 井橋 俊哉; 鷹巣 篤志; 坂上 凛; 林 真那; 西崎俊太朗; 宮川靖基; 森龍之介; 門野莉紗; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • identification of maternal transcripts that can the development potential of mammalian embryos for predicting full-term development  [Not invited]
    井上 明裕; Nicole Cheung; 山本真理; 塚口智将; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜子; 池田 智明; 山之内 忠幸; 松田 秀雄; 井橋 俊哉; 鷹巣 篤志; 坂上 凛; 林 真那; 西崎俊太朗; 宮川靖基; 森龍之介; 門野莉紗; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Functional analyses of the H3K4me3 modification in chromosomes of mouse oocytes at the metaphase II stage  [Not invited]
    鷹巣篤志; 日野敏昭; 三村知也; 梁智華; 伊田ちさと; 宮川靖基; 坂上凜; 門野莉紗; 井上明裕; 西崎俊太朗; 森龍之介; 井橋俊哉; 松本和也; 的場彰悟; 小倉淳郎; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Transcriptional reprogramming of wild animal cell nuclei  [Not invited]
    Risa Kadono; Christopher Penfold; Syunya Ihashi; Atsushi Takasu; Rin; Sakanoue; Mana Hayashi; Akihiro Inoue; Shuntaro Nishizaki; Yasuki Miyagawa; Ryunosuke Mori; Kazuya Matsumoto; Masayuki Anzai; Kei Miyamoto
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07  神奈川県足柄下郡湯河原町鍛冶屋 572-1 レクトーレ湯河原
  • Effects of transient degradation of Lamin B1 during the 2-cell stage in early mouse embryos  [Not invited]
    Rin Sakanoue; Masahito Tanaka; Atsushi Takasu; Yasuki Miyagawa; Naoko Watanabe; Yuta Shimamoto; Kei Miyamoto
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Functions of nuclear actin in mouse embryonic development  [Not invited]
    宮川靖基; 坂上凜; 眞銅大暉; 坂本裕子; 三島花心; 井橋俊哉; 鷹巣篤志; 林真那; 井上明裕; 門野莉紗; 西崎俊太朗; 森龍之介; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023(ポスター発表)  2023/07
  • Functions of nuclear actin in mouse embryonic development  [Not invited]
    宮川靖基; 坂上凜; 眞銅大暉; 坂本裕子; 三島花心; 井橋俊哉; 鷹巣篤志; 林真那; 井上明裕; 門野莉紗; 西崎俊太朗; 森龍之介; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Rejuvenation of senescence mouse cells using oocyte factors  [Not invited]
    西崎 俊太朗; 井橋俊哉; 森龍之介; 鷹巣篤志; 林真那; 坂上凜; 井上明裕; 宮川靖基; 門野莉紗; 小藪直生; 藤原香凜; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Transcriptional reprogramming of wild animal cell nuclei  [Not invited]
    Risa Kadono; Christopher Penfold; Syunya Ihashi; Atsushi Takasu; Rin Sakanoue; Mana Hayashi; Akihiro Inoue; Shuntaro Nishizaki; Yasuki Miyagawa; Ryunosuke Mori; Kazuya Matsumoto; Masayuki Anzai; Kei Miyamoto
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07  〒259-0313 神奈川県足柄下郡湯河原町鍛冶屋 572-1 レクトーレ湯河原
  • Age Reprogramming of senescent cells using Somatic Cell Nuclear Transfer  [Not invited]
    森龍之介; 西崎俊太朗; 井橋俊哉; 鷹巣篤志; 林真那; 坂上凜; 井上明裕; 門野莉紗; 宮川靖基; 松本和也; 宮本圭
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • Nuclear actin assembly is an integral part of decidualization in human endometrial stromal cells  [Not invited]
    Kei Miyamoto
    文部科学省 科学研究費補助金 新学術領域研究 「全能性プログラム」若手勉強会 2023  2023/07
  • 非クロマチン核内因子による核機能の制御と胚発生における役割  [Invited]
    宮本圭
    山口大学医学部産科婦人科 産婦人科セミナー  2022/12
  • Examination of genetic markers for the purpose of establishing a method for evaluating the quality of mouse and bovine embryos.  [Not invited]
    AKIHIRO INOUE; MARI YAMAMOTO; TADAYUKI; YAMANOUCHI; HIDEO MATSUDA; SHUNYA IHASHI; TAIKI SHINDO; ATSUSHI TAKASU; MANA HAYASHI,RIN SAKANOUE; KAZUYA MATSUMOTO; KEI MIYAMOTO
    第45回日本分子生物学会  2022/12
  • Quantitative assessment of expression dynamics of nucleoskeleton protein lamins in early mouse embryos  [Not invited]
    RIN SAKANOUE; MASAHITO TANAKA; TAIKI SHINDO; YASUKI MIYAGAWA; MARI YAMAMOTO; SYUNYA; IHASHI,ATSUSHI TAKASU; MANA HAYASHI; KAZUYA MATSUMOTO; YUTA SHOMAMOTO; KEI MIYAMOTO
    第45回日本分子生物学会  2022/12
  • Zygotic genome activation pathway mediated by nuclear F-actin  [Not invited]
    Taiki Shindo; Yuko Sakamoto; Rin Sakanoue; Yasuki Miyagawa; Mari Yamamoto; Syunya Ihashi; Atsushi Takasu; Mana Hayashi; Akihiro Inoue; Ryunosuke Mori; Syuntaro Nishizaki; Risa Kadono; Kazuya Matsumoto; Kei Miyamoto
    第45回日本分子生物学会  2022/12
  • Mechanism of developmental arrest in Alyref and Gabpb1 knockouts, genes necessary for preimplantation development of early embryonic development.  [Not invited]
    Shunya Ihashi; Ryunosuke Mori; Shuntarou Nishizaki; Misaki Nakamura; Mizuto Hamanaka; Masaya Kaji; Miki Mori; Yuma Imasato; Mari Yamamoto; Taiki Shindo; Atushi Takasu; Rin Sakanoue; Masayuki Anzai; Kazuya Matsumoto; Masahito Ikawa; Kei Miyamoto
    第45回日本分子生物学会  2022/12
  • Roles of the actin nucleoskeleton in mouse embryonic development  [Invited]
    Kei Miyamoto
    第45回日本分子生物学会  2022/11
  • Dynamic expression of nucleoskeleton proteins regulates mouse preimplantation development  [Invited]
    Kei Miyamoto
    The International Symposium "Totipotency and Germ Cell Development"  2022/11
  • Incomplete activation of developmentally required genes Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos  [Not invited]
    Shunya Ihashi; Mizuto Hamanaka; Masaya Kaji; Ryunosuke Mori; Shuntaro Nishizaki; Miki Mori; Yuma Imasato; Atsushi Takasu; Misaki Nakamura; Kazuya Matsumoto; Masayuki Anzai; Masahito Ikawa; Kei Miyamoto
    The International Symposium "Totipotency and Germ Cell Development"  2022/11
  • Identification and utilization of maternal transcripts associated with the developmental potential of mammalian embryos  [Not invited]
    井上明裕; 山之内忠幸; 松田秀雄; 山本真理; 井橋俊哉; 鷹巣篤志; 坂上凜; 崎俊太朗; 宮川靖基; 森龍之介; 松本和也; 宮本圭
    新学術・学術変革領域合同 若手の会 2022  2022/11
  • マウス初期胚におけるLaminの定量的発現動態解析  [Not invited]
    坂上凜; 田中真己; 宮川靖基; 島本勇太; 宮本圭
    新学術・学術変革領域合同 若手の会 2022  2022/11
  • Roles of Alyref and Gabpb1 genes in mouse somatic cell nuclear transfer embryos  [Not invited]
    西崎俊太朗; 井橋俊哉; 森龍之介; 山本真理; 眞銅大輝; 林真那; 鷹巣篤志; 坂上凜; 井上明裕; 宮川靖基; 松本和也; 伊川正人; 宮本圭
    新学術・学術変革領域合同 若手の会 2022  2022/11
  • 全能性獲得に関与するAlyref・Gabpb1遺伝子のマウス初期胚発生における役割  [Not invited]
    森龍之介; 井橋俊哉; 西崎俊太朗; 山本真理; 鷹巣篤志; 坂上凜; 井上明裕; 宮川靖基; 松本和也; 伊川正人; 宮本圭
    新学術・学術変革領域合同 若手の会 2022  2022/11
  • Visualization and functional analysis of zygotic nuclear F-actin  [Not invited]
    宮川靖基; 坂本裕子; 坂上凛; 眞銅大暉; 井橋俊哉; 鷹巣篤志; 山本真理; 森龍之介; 西崎俊太朗; 井上明裕; 門野莉紗; 松本和也; 宮本圭
    新学術・学術変革領域合同 若手の会 2022  2022/11
  • ウシ母性転写産物量を指標とした胚の品質評価法の検討  [Not invited]
    井上 明裕; 山之内 忠幸; 松田 秀雄; 山本 真理; 井橋 俊哉; 眞銅 大暉; 鷹巣 篤志; 林 真那; 坂上 凛; 松本 和也; 宮本 圭
    第115回 日本繁殖生物学会大会  2022/09
  • マウス初期胚における核骨格構造の動態解析  [Not invited]
    坂上 凜; 眞銅 大暉; 坂本 裕子; 山本 真理; 井橋 俊哉; 鷹巣 篤志; 林 真那; 松本 和也; 宮本 圭
    第115回 日本繁殖生物学会大会  2022/09
  • Single-cell RNA-seq法を用いた体外成熟ヒト卵のトランスクリプトーム解析  [Not invited]
    山本 真理; 武内 大輝; 福井 愛実; 井上 明裕; 前沢 忠志; 西岡 美喜子; 近藤 英司; 池田 智明; 松本 和也; 宮本 圭; 坂上 凜; 林 麻耶; 眞銅 大暉; 鷹巣 篤志; 井橋 俊哉
    第115回 日本繁殖生物学会大会  2022/09
  • AlyrefおよびGabpb1遺伝子の活性化不全はマウスクローン胚の着床前致死を導く  [Not invited]
    井橋 俊哉; 濱中 瑞斗; 加地 正弥; 森 美樹; 今里 佑馬; 中村 岬; 安齋 政幸; 松本 和也; 伊川 正人; 宮本 圭
    第115回 日本繁殖生物学会大会  2022/09
  • 体細胞核の全能性獲得に関与する遺伝子の同定と着床前発生における機能  [Invited]
    井橋 俊哉; 中村 岬; 安齋 政幸; 松本 和也; 伊川 正人; 宮本 圭
    第74回日本細胞生物学会大会  2022/06
  • Nuclear F-actin for embryonic development and nuclear reprogramming  [Invited]
    Kei Miyamoto
    1st Nuclear Actin Symposium  2022/05
  • 体外成熟培養に供試したヒト卵のトランスクリプトーム解析  [Not invited]
    山本真理; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜; 池田 智; 松本和也; 宮本圭
    第10 回 関西生殖集談会 第54 回 関西アンドロロジーカンファレンス 合同研究会  2022/03
  • 低侵襲的遺伝子発現解析による卵質の評価  [Invited]
    宮本圭
    第12回日本がん・生殖医療学会学術集会  2022/02
  • Reprogramming of gene expression in embryonic development  [Invited]
    Kei Miyamoto
    Graduate course is on chromatin organization and dynamics during differentiation, Stockholm University  2021/12
  • RNAポリメラーゼIIの転写伸長反応が転写リプログラミングの律速段階となる  [Not invited]
    林真那; 辻本佳加理; 鹿喰巧磨; 大石真生; 白水宗; 山本真理; 井橋俊哉; 眞銅大暉; 坂上凜; 松本和也; Robert Grosse; 宮本圭
    第44回日本分子生物学会年会  2021/12
  • マウス初期胚における核骨格タンパク質の発現動態  [Not invited]
    坂上凜; 眞銅大暉; 坂本裕子; 山本真理; 井橋俊哉; 林真那; 松本和也; 宮本圭
    第44回日本分子生物学会年会  2021/12
  • 受精卵発生に必須な遺伝子の過剰発現が体細胞核移植胚の発生に及ぼす影響  [Not invited]
    井橋俊哉; 中村岬; 黒田岳; 曽我部桃佳; 濱中瑞斗; 加地正弥; 日下部春奈; 森美樹; 今里佑馬; 松澤由佳; 梶栗尚明; 山本真理; 眞銅大暉; 林 真那; 坂上 凜; 松本和也; 伊川正人; 宮本圭
    第44回日本分子生物学会年会  2021/12
  • 体外成熟培養ヒト卵のトランスクリプトームプロファイル  [Not invited]
    山本 真理; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜子; 池田 智明; 松本 和也; 宮本 圭
    第44回日本分子生物学会年会  2021/12
  • 全能性細胞核の構築や機能に関する研究  [Invited]
    宮本圭
    2021年度新学術全能性領域会議  2021/11
  • ヒト卵の成熟過程におけるシングルセル遺伝子発現プロファイリング  [Invited]
    宮本 圭
    第66回日本生殖医学会学術講演会・総会  2021/11
  • ノーベル賞受賞者のラボでの研究生活  [Invited]
    宮本圭
    114 回 日本繁殖生物学会  2021/09
  • Transcriptional reprogramming of somatic cells derived from an endangered animal using a novel nuclear transfer system  [Invited]
    Junko Tomikawa; Christopher Penfold; Takuma Kamiya; Risa Hibino; Masayuki Anzai; Kazuya Matsumoto; Kei Miyamoto
    第92回日本動物学会オンライン 米子大会  2021/09
  • 哺乳動物胚の着床前発生における遺伝子発現リプログラミング機構  [Invited]
    宮本 圭
    第39回受精着床学会総会・学術講演会  2021/07
  • 核内アクチンタンパク質による核構造と遺伝子発現の制御  [Invited]
    宮本圭
    第33回バイオエンジニアリング講演会  2021/06
  • 卵内因子によるクロマチン構造と転写状態の初期化  [Invited]
    宮本 圭
    第14回日本エピジェネティクス研究会年会  2021/03
  • The actin-family protein Arp4 suppresses formation of nuclear actin filament  [Not invited]
    ○Yuya UENO; Shota YAMAZAKI; Christian GERHOLD; Koji YAMAMOTO; Kei MIYAMOTO; Masahiko HARATA
    日本農芸化学会2021年度大会  2021/03
  • ヒト卵の体外成熟過程におけるトランスクリプトーム解析  [Not invited]
    武内大輝; 山本真理; 前沢忠志; 西岡美喜子; 池田智明; 松本和也; 宮本圭
    新学術領域研究『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム2020  2020/12
  • Visualization of endogenous nuclear F-actin in mouse embryos reveals abnormal actin assembly after somatic cell nuclear transfer  [Not invited]
    眞銅大暉; 井橋俊哉; 坂本裕子; 奥野智美; 冨川順子; 宮本圭
    新学術領域研究『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム2020  2020/12
  • 体細胞核移植における全能性獲得に関与する遺伝子の機能解析 -Alyref および Gabpb1 の初期胚発生における役割-  [Not invited]
    井橋俊哉; 濱中瑞斗; 加地正弥; 森美樹; 今里佑馬; 日下部春奈; 梶栗尚明; 松澤由佳; 山本真理; 坂本裕子; 辻本佳加理; 笠原喜斗; 眞銅大暉; 松本和也; 伊川正人; 宮本圭
    新学術領域研究『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム2020  2020/12
  • 宮本圭
    新学術領域研究『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム2020  2020/12
  • Gabpb1遺伝子のマウス初期胚発生における役割  [Not invited]
    井橋俊哉; 濱中瑞斗; 加地正弥; 森美樹; 今里佑馬; 日下部春奈; 梶栗尚明; 松澤由佳; 山本真理; 坂本裕子; 辻本佳加理; 笠原喜斗; 眞銅大暉; 松本和也; 伊川正人; 宮本圭
    第43回 日本分子生物学会年会  2020/12
  • Gabpb1遺伝子のマウス初期胚発生における機能解析  [Not invited]
    井橋俊哉; 濱中瑞斗; 加地正弥; 森美樹; 今里佑馬; 日下部春奈; 梶栗尚明; 松澤由佳; 山本真理; 坂本裕子; 辻本佳加理; 笠原喜斗; 眞銅大暉; 松本和也; 伊川正人; 宮本圭
    第113回 日本繁殖生物学会大会  2020/09
  • マウス初期胚を用いた体細胞核の転写リプログラミング誘導  [Invited]
    宮本圭
    第113回 日本繁殖生物学会大会  2020/09  青葉山新キャンパス 動物生殖科学分野(Web開催)  大会長:種村 健太郎先生(東北大学大学院農学研究科)
  • マウス初期胚を用いた新規核移植法による直接的な転写リプログラミング誘導  [Invited]
    宮本圭
    第93回 日本生化学会大会  2020/09
  • 「サイエンスクエスト そして科学者へ・・・」  [Invited]
    宮本圭
    和歌山県高等学校生徒科学研究発表会  2019/12  和歌山県民文化会館
  • 受精卵の全能性を評価する方法の開発に向けて  [Not invited]
    山本 真理; Nicole Cheung; 塚口 智将; 小林 久人; 神尾 明日香; 奥野 智美; 神谷 拓磨; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 坂本 裕子; 笠原 善斗; 眞銅 大暉; 河野 友宏; 松本 和也; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • 核移植における体細胞核の全能性獲得に関与する遺伝子の機能解析  [Not invited]
    井橋 俊哉; 森 美樹; 今里 佑馬; 日下部 春奈; 梶栗 尚明; 松澤 由佳; 加地 正弥; 濱中 瑞斗; 神谷 拓磨; 奥野 智美; 山 本 真理; 越智 浩介; 坂本 裕子; 辻本 佳加理; 笠原 喜斗; 眞銅 大暉; 松橋 珠子; 伊川 正人; 松本 和也; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • マウス初期胚発生における受精卵特異的重合化核アクチンの機能解析  [Not invited]
    奥野 智美; Li Wayne Yang; 波多野 裕; 鷹巣 篤志; 山本 真理; 池田 善貴; Plessner Matthias; 坂本 裕子; 守田 昂太; 郎; 松本 和也; 山縣 一夫; Grosse Robert; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • 細胞周期を停止したマウス初期胚による核リモデリング  [Not invited]
    神谷 拓磨; 西浦 伊織; 奥野 智美; 山本 真理; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 坂本 裕子; 笠原 善斗; 眞銅 大暉; 松橋 珠子; 松本 和也; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • 核内アクチンタンパク質の重合化がマウス初期胚の細胞分裂に及ぼす影響  [Not invited]
    坂本 裕子; 奥野 智美; Li Yang; 山本 真理; 神谷 拓磨; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 笠原 喜斗; 眞銅 大暉; 松 橋 珠子; 松本 和也; Robert Grosse; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • カエル卵母細胞を用いた転写リプログラミング機構の解析  [Not invited]
    辻本 佳加理; 白水 宗; 小林 智輝; 西 満里奈; 辻村 翔子; 神谷 拓磨; 奥野 智美; 山本 真理; 越智 浩介; 井橋 俊哉; 坂本 裕子; 笠原 喜斗; 眞銅 大暉; 松橋 珠子; 松本 和也; Grosse Robert; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • マウス初期胚発生における受精卵特異的重合化核アクチンの機能解析  [Not invited]
    奥野 智美; Li Wayne Yang; 波多野 裕; 鷹巣 篤志; 山本 真理; 池田 善貴; Matthias Plessner; 坂本 裕子; 守田 昂太郎; 松本 和也; 山縣 一夫; Robert Grosse; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • マウス受精卵前核の機能維持における核骨格タンパク質の役割  [Invited]
    奥野 智美; Yang Wayne Li; 波多野 裕; 鷹巣 篤志; 山本 真理; 池田 善貴; Matthias Plessner; 坂本 裕子; 守田 昂太郎; 松本 和也; 山縣 一夫; Robert Grosse; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • マウス受精卵を用いた内在性核内繊維状アクチンの可視化法の検討  [Not invited]
    眞銅 大暉; 坂本 裕子; 奥野 智美; Li Yang; 山本 真理; 神谷 拓磨; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 笠原 喜斗; 松 橋 珠子; 松本 和也; Robert Grosse; 宮本 圭
    第42回日本分子生物学会年会  2019/12
  • 肉用牛の出荷時の筋肉内脂肪中のオレイン酸含有割合を予測する血中バイオマーカータンパク質の検討  [Not invited]
    越智 浩介; 池上 春香; 松橋 珠子; 邨上 正幸; 山本 真理; 笠原 喜斗; 眞銅 大暉; 宮本 圭; 加藤 博己; 高取 等; 松本 和也
    第42回日本分子生物学会年会  2019/12
  • 光遺伝学ツールを用いた核アクチン重合化の促進と転写リプログラミングにおけるクロマチンタンパク質の動態  [Not invited]
    辻本佳加理; 白水宗; 小林智輝; 西満里奈; 辻村翔子; 守屋朱香; 國富瑞生; 松橋珠子; 松本和也; Robert Grosse; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • マウス初期胚における核内アクチンタンパク質と初期細胞分裂の関係性  [Not invited]
    坂本裕子; 奥野智美; Wayne Yang Li; 眞銅太暉; 鷹巣篤志; 山本真理; 波多野裕; 池田善貴; Matthias Plessner; 守田昂太郎; 松本和也; 山縣一夫; Robert Grosse; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • Functional analysis of genes responsible for acquiring totipotency by somatic cell nuclear transfer  [Not invited]
    井橋 俊哉; 濱中瑞斗; 加地正弥; 森美樹; 今里佑馬; 日下部春奈; 梶栗尚明; 松澤由佳; 神谷拓磨; 奥野智美; 山本真理; 越智浩介; 坂本裕子; 辻本佳加理; 笠原喜斗; 松橋珠子; 松本和也; 伊川正人; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • Transcriptional reprogramming induced by a novel nuclear transfer system that does not require cell division and DNA replication  [Not invited]
    神谷拓磨; Christopher A. Penfold; 奥野智美; 山本真理; 越智浩介; 井橋俊哉; 辻本佳加理; 坂本裕子; 松橋珠子; 松本和也; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • マウス前核期胚における重合化核アクチンの機能解明に向けて  [Not invited]
    奥野智美; Wayne Yang Li; 波多野裕; 鷹巣篤志; 山本真理; 池田善貴; Matthias Plessner; 坂本裕子; 守田昂太郎; 松本和也; 山縣一夫; Robert Grosse; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • 受精卵の全能性に関する母性転写産物の探索  [Not invited]
    山本真理; 宮本圭
    全能性プログラム:デコーディングからデザインへ 若手勉強会  2019/11
  • 核内アクチン重合化は受精卵前核の機能維持と初期胚発生に必要である  [Invited]
    宮本圭
    全能性プログラム:デコーディングからデザインへ キックオフシンポジウム  2019/11
  • 分子マーカーを用いた受精卵の発生能予測  [Invited]
    宮本圭
    第64回 日本生殖医学会学術講演会・総会  2019/11
  • 光遺伝学ツールを用いた核アクチン重合化の促進と転写リプログラミングにおけるHP1ファミリータンパク質の動態  [Not invited]
    辻本佳加理; 白水宗; 小林智輝; 西満里奈; 辻村翔子; 神谷拓磨; 奥野智美; 山本真理; 越智浩介; 井橋俊哉; 坂本裕子; 笠原喜斗; 松橋珠子; 松本和也; Robert Grosse; 宮本圭
    第92回日本生化学会大会  2019/09  パシフィコ横浜
  • 受精卵特異的重合化核アクチンの機能解析  [Not invited]
    奥野智美; Wayne Yang Li; 波多野裕; 鷹巣篤志; 山本真理; 池田善喜; Matthias Plessner; 坂本裕子; 守田昂太郎; 松本和也; 山縣一夫; Robert Grosse; 宮本圭
    第92回日本生化学会大会  2019/09  パシフィコ横浜
  • A novel nucleoskeleton structure in mouse zygotes and its developmental functions  [Invited]
    宮本圭
    第92回日本生化学会大会  2019/09
  • 核移植における体細胞核の全能性獲得に関与する遺伝子の探索  [Not invited]
    井橋 俊哉; 森 美樹; 今里 佑馬; 日下部 春奈; 梶栗 尚明; 松澤 由佳; 神谷 拓磨; 奥野 智美; 山本 真理; 越智 浩介; 坂本 裕子; 辻本 佳加理; 笠原 善斗; 松橋 珠子; 松本 和也; 宮本 圭
    第112回日本繁殖生物学会大会  2019/09
  • 受精卵の発生能を予測するシステムの開発  [Not invited]
    山本 真理; Nicole CHEUNG; 塚口 智将; 小林 久人; 神尾 明日香; 奥野 智美; 神谷 拓磨; 越智 浩介; 井橋 俊也; 辻本 佳加理; 坂本 裕子; 笠原 善斗; 眞銅 大暉; 河野 友宏; 松本 和也; 宮本 圭
    第112回日本繁殖生物学会大会  2019/09
  • 3種類の化合物の組み合わせがブタ核移植胚の発生能に及ぼす影響  [Not invited]
    岩元 正樹; 矢崎 智子; 井橋 俊哉; 山田 雅保; 宮本 圭
    第112回日本繁殖生物学会大会  2019/09
  • 受精後の母性タンパク質PIASyの分解は母性から胚性への移行に重要である  [Not invited]
    樋口 智香; 山本 真理; 奥野 智美; 神谷 拓磨; 越智 浩介; 宮本 圭; 松本 和也
    第112回 日本繁殖生物学会大会  2019/09
  • 受精卵の発生能を予測するシステムの開発について  [Not invited]
    山本真理; Nicole Cheung; 塚口智将; 小林久人; 神尾明日香; 奥野智美; 神谷拓磨; 越智浩介; 井橋俊也; 辻本佳加理; 坂本裕子; 笠原善斗; 眞銅大暉; 河野友宏; 松本和也; 宮本圭
    第3回日本胚移植技術研究会大会(和歌山大会)  2019/08
  • Proteomic analysis of muscle and bone marrow tissues obtained from a 28,000- year-old woolly mammoth  [Not invited]
    永井 宏平; 宮本 裕史; 安齋 政幸; 東 里香; 西端 智也; 山縣 一夫; 加藤 博己; 宮本 圭; Kolodeznikov Igor I. Protopopov; Albert V. Plotnikov Valerii V; 細井 美彦; 三谷 匡; 松本 和也; 入谷 明
    日本プロテオーム学会2019年大会  2019/07
  • Comprehensive analysis of post-translational modifications on the proteins from a 28,000-year-old woolly mammoth  [Not invited]
    西端 智也; 永井 宏平; 山縣 一夫; 宮本 裕史; 安齋 政幸; 加藤 博己; 宮本 圭; 東 里 香; Kolodeznikov Igor; I. Protopopov; Albert V; Plotnikov Valerii V; 細井 美彦; 三谷 匡; 松本 和也; 入谷 明
    日本プロテオーム学会2019年大会  2019/07
  • Novel roles of nuclear actin polymerization in establishing nuclear structures and in embryonic development  [Not invited]
    宮本圭
    19th HFSP Awardees Meeting and 30th anniversary celebration  2019/07
  • 転写リプログラミングにおけるクロマチン構造変化の階層的理解  [Not invited]
    宮本圭
    第2回クロマチン潜在能領域会議  2019/06
  • カエル卵母細胞を用いた転写リプログラミング機構の解析  [Not invited]
    辻本佳加理; 白水宗; 小林智輝; 西満里奈; 辻村翔子; 神谷拓磨; 奥野智美; 山本真理; 越智浩介; 井橋俊哉; 坂本裕子; 笠原喜斗; 松橋珠子; 松本和也; Robert Grosse; 宮本圭
    第2回クロマチン潜在能領域  2019/06
  • マウス受精卵における重合化核アクチンの役割  [Not invited]
    奥野智美; Wayne Yang Li; 波多野裕; 鷹巣篤志; 山本真理; 池田善喜; Matthias Plessner; 坂本裕子; 守田昂太郎; 松本和也; 山縣一夫; Robert Grosse; 宮本圭
    第2回クロマチン潜在能領域会議  2019/06
  • 核内アクチンタンパク質の重合化がマウス初期胚の細胞分裂に及ぼす影響  [Not invited]
    坂本 裕子; 奥野 智美; Yang Li; 山本 真理; 神谷 拓磨; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 松橋 珠子; 松本 和也; Robert Grosse; 宮本 圭
    第60回日本卵子学会学術集会  2019/05
  • 低侵襲的に受精卵の発生能を予測する新規システムの開発に向けて  [Invited]
    宮本圭
    第15回東海ARTカンファレンス  2019/02
  • 受精卵の全能性を予測するシステムの開発にむけて  [Not invited]
    山本真理; Nicole Cheung; 塚口智将; 小林久人; 神尾明日香; 奥野智美; 神谷拓磨; 越智浩介; 樋口智香; 井橋俊也; 辻本佳加理; 坂本裕子; 河野友宏; 松本和也; 宮本圭
    生物資源ゲノム解析拠点 研究報告会  2019/02
  • Actin polymerization in reprogramming nuclear structures  [Invited]
    Kei Miyamoto
    ASCB/EMBO 2018meeting  2018/12  San Diego Convention Center
  • マウス体細胞核移植胚と受精卵の全能性獲得に関わる分子機構  [Invited]
    宮本圭
    新学術領域研究 成果取りまとめ公開シンポジウム「生殖細胞のエピゲノムダイナミクスとその制御」  2018/12  京都教育文化センター
  • 肉用牛の出荷時の筋肉内脂肪中のオレイン酸含有割合を生体評価する血中バイオマーカータンパク質の検討  [Not invited]
    越智 浩介; 池上 春香; 松橋 珠子; 邨上 正幸; 樋口 智香; 奥野 智美; 神谷 拓磨; 山本 真理; 井橋 俊哉; 坂本 裕子; 辻本 佳加理; 宮本 圭; 永井 宏平; 加藤 博; 高取 等; 松本 和; 近大院生物理工; 近大生物理工; 近大先端研; 鳥取畜試
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • 核移植における体細胞核の全能性獲得に関与する遺伝子の探索  [Not invited]
    井橋 俊哉; 森 美樹; 今里 佑馬; 日下部 春奈; 梶栗 尚明; 松澤 由佳; 樋口 智香; 神谷 拓磨; 奥野 智美; 山本 真理; 越智 浩介; 坂本 裕子; 辻本 佳加理; 松橋 珠子; 松本 和也; 宮本; 近大; 生物理工; 近大; 先技総研
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • レトロトランスポゾンMERVLの活性化機構の解析  [Not invited]
    奥野 智美; 山口 壮輝; 樋口 智香; 神谷 拓磨; 山本 真理; 越智 浩介; 西野 亜理紗; 井橋 俊哉; 辻本 佳加理; 坂本 裕子; 松橋 珠子; 安齋 政幸; 黒坂 哲; 三谷 匡; 山縣 一夫; 細井 美彦; 松本 和也; 宮本 圭; 近畿大学生物理工学部; 近大先技総
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • 細胞周期を停止したマウス初期胚による転写リプログラミング  [Not invited]
    神谷 拓磨; 西浦 伊織; 本上 遥; 久米 健太; 樋口 智香; 奥野 智美; 山本 真理; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 坂本 裕子; 松橋 珠子; 細井 美彦; 松本 和也; 宮本 圭; 近畿大学生物理工; 近大先技総
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • 単一受精卵からの網羅的遺伝子発現解析法の最適化  [Not invited]
    山本 真理; チャン ニコール; 塚口 智将; 小林 久人; 神尾 明日香; 奥野 智美; 神谷 拓磨; 越智 浩介; 樋口 智香; 井橋 俊也; 辻本 佳加理; 坂本 裕子; 河野 友宏; 松本 和也; 宮本 圭; 近大院生物理工; 東農大; 生物資源ゲノムセンタ
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • 光遺伝学ツールを用いた核アクチン重合化の促進と卵母細胞における転写リプログラミングへの影響  [Not invited]
    辻本 佳加理; 白水 宗; 小林 智輝; 西 満里奈; 辻村 翔子; 樋口 智香; 神谷 拓磨; 奥野 智美; 山本 真理; 越智 浩介; 井橋 俊哉; 坂本 裕子; 松橋 珠子; 松本 和也; 宮本 圭; 近大生物理工; 近大先技総
    第41回日本分子生物学会年会  2018/11  パシフィコ横浜
  • Roles of actin family proteins in chromatin and nuclear functions  [Not invited]
    Nanako Machida; Shota Yamazaki; Yusuke Akiyama; Kei Miyamoto; Bertoldo Davide; Christian Heinis; Masahiko Harata
    International Symposium on 3R and 3C  2018/11  金沢市文化ホール  開催責任者:白髭克彦(東京大学分子細胞生物学研究所・教授)
  • 初期胚特異的レトロトランスポゾンMERVLの活性化機構の解析  [Not invited]
    奥野 智美; 樋口 智香; 神谷 拓磨; 山本 真理; 越智 浩介; 西野 亜理紗; 井橋 俊哉; 辻本 佳加理; 松橋 珠子; 安齋 政幸; 黒坂 哲; 三谷 匡; 山縣 一夫; 細井 美彦; 松本 和也; 宮本 圭
    第111回 日本繁殖生物学会大会  2018/09
  • 細胞分裂及びDNA複製非依存的リプログラミングシステムの構築  [Not invited]
    神谷 拓磨; 本上 遥; 久米 健太; 樋口 智香; 奥野 智美; 山本 真理; 越智 浩介; 井橋 俊哉; 辻本 佳加理; 松橋 珠子; 細井 美彦; 松本 和也; 宮本 圭
    第111回 日本繁殖生物学会大会  2018/09
  • 受精後にユビキチン・プロテアソーム系による分解が必要な母性タンパク質の同定  [Not invited]
    樋口 智香; 奥野 智美; 神谷 拓磨; 山本 真理; 越智 浩介; 宮本 圭; 松本 和也
    第111回 日本繁殖生物学会大会  2018/09
  • Nuclear actin polymerization in reprogramming nuclear structures  [Invited]
    宮本圭
    国立遺伝学研究所(セミナー)  2018/09
  • DNA複製非依存的リプログラミングシステムの構築(A novel reprogramming system that does not require DNA replication)  [Not invited]
    神谷拓磨; 本上遥; 久米健太; 山口壮輝; 守田昂太郎; 樋口智香; 奥野智美; 山縣一夫; 細井美彦; 松本和也; 宮本圭
    2017年度生命科学系学会合同年次大会  2017/12
  • 初期胚特異的レトロトランスポゾンMERVLの活性化に伴う核内変化  [Not invited]
    奥野智美; 山口壮輝; 濱田歩花; 竹林真理愛; 守田昂太郎; 樋口智香; 神谷拓磨; 安齋政幸; 山縣一夫; 細井美彦; Jerome Jullien; 松本和也; 宮本圭
    2017年度生命科学系学会合同年次大会  2017/12
  • Roles of nuclear actin in mouse embryonic development  [Invited]
    宮本圭
    2017年度生命科学系学会合同年次大会(ConBio2017)  2017/12
  • Nuclear actin polymerization in mouse embryonic development  [Invited]
    宮本圭
    ASCB/EMBO2017meeting  2017/12
  • 核アクチンのマウス初期胚発生における役割  [Not invited]
    守田昂太郎; 鷹巣篤志; 波多野裕; 簾新智; プレッスナーマティアス; 松本和也; 山縣一夫; グロッセロバート; 宮本圭
    第40回日本分子生物学会年会  2017/12
  • 核の初期化とマウス初期胚発生における核アクチンの役割  [Invited]
    宮本圭
    新学術領域研究・第5回公開シンポジウム  2017/11
  • Roles of nuclear actin in nuclear reprogramming and mouse embryonic development  [Not invited]
    Atsushi Takasu; Kohtaro Morita; Yu Hatano; Yang Li; Tomoki Kobayashi; Marina Nishi; Shoko Tsujimura; Shinji Misu; Matthias Plessner; Kazuya Matsumoto; Robert Grosse; Kazuo Yamagata; Kei Miyamoto
    第5回公開シンポジウム 生殖細胞のエピゲノムダイナミクスとその制御  2017/11
  • クローン動物から再生医療へ~細胞の初期化がもたらす未来~  [Invited]
    宮本圭
    平成29年度中央区民カレッジ  2017/10
  • Methylation of arginine 2 in histone H3.3 is important for minor zygotic genome activation in mouse pronuclear embryos  [Not invited]
    KohtaroMorita; Chika Higuchi; SokiYamaguchi; Tomomi Okuno; Takuma Kamiya; Tamako Matsuhashi; KoheiNagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    Histone variants: Molecular functions in health and disease Biomedical Center (BMC)  2017/09
  • Roles of nuclear actin in nuclear reprogramming and mouse embryonic development  [Invited]
    宮本圭
    HMGU-Japan Epigenetics and Chromatin Symposium  2017/09
  • Methylation of arginine 2 in histone H3.3 is important for minor zygotic genome activation in mouse pronuclear embryos  [Not invited]
    KohtaroMorita; Chika Higuchi; SokiYamaguchi; Tomomi Okuno; Takuma Kamiya; Tamako Matsuhashi; KoheiNagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    1st München-Japan Mini Symposium  2017/09
  • Involvement of H3R2me2s in mouse preimplantation development.  [Not invited]
    Kohtaro Morita; Chika Higuchi; Soki Yamaguchi; Tamako Matsuhashi; Kouhei Nagai; Masayuki Anzai; Hiromi Kato; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    Fourth World Congress of Reproductive Biology (WCRB2017)  2017/09
  • Proper degradation of a maternal protein during maternal-to-zygotic transition is important for normal development.  [Not invited]
    Chika Higuchi; Kohtaro Morita; Soki Yamaguchi; Tamako Matsuhashi; Kohei Nagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    Fourth World Congress of Reproductive Biology  2017/09
  • Open chromatin configuration at primed promoters is formed during embryogenesis and accelerates transcriptional activation during differentiation and nuclear reprogramming  [Not invited]
    宮本圭
    EMBO Conference The Nucleosome:From Atoms to Genomes  2017/08
  • マウス初期胚発生の進行に重要な受精後に分解される母性タンパク質の探索  [Not invited]
    樋口智香; 守田昂太郎; 山口壮輝; 奥野智美; 神谷拓磨; 松橋珠子; 永井宏平; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    新学術領域「生殖細胞のエピゲノムダイナミクスとその制御」3領域合同若手勉強会  2017/06
  • 受精後のユビキチン・プロテアソーム系による母性タンパク質の分解はマウス初期胚発生に重要である  [Not invited]
    樋口 智香; 守田 昂太郎; 山口 壮輝; 松橋 珠子; 永井 宏平; 安齋 政幸; 山縣 一夫; 細井 美彦; 宮本 圭; 松本 和也
    第58回日本卵子学会  2017/06
  • マウス初期胚発生過程におけるH3R2me2s の役割  [Not invited]
    守田 昂太郎; 樋口 智香; 山口 壮輝; 松橋 珠子; 永井 宏平; 安齋 政幸; 加藤 博己; 山縣 一夫; 細井 美彦; 宮本 圭; 松本 和也
    第58回日本卵子学会  2017/06
  • ヒストン脱アセチル化酵素阻害剤が異種間核移植胚の発生に与える影響  [Not invited]
    東里香; 村井仁志; 小笠原里奈; 小木曽力; 鷲津朱理; 宮本圭; 宮下実; 松本和也; 細井美彦; 安齋政幸
    第64回日本実験動物学会総会  2017/05
  • クローン動物がもたらした可能性  [Invited]
    宮本圭
    平成29年度大阪府高等学校生物教育研究会  2017/05
  • Efficient nuclear reprogramming of somatic cells towards totipotency is supported by synergistic effects of small molecules  [Invited]
    K. Miyamoto; Y. Tajima; K. Yoshida; M. Oikawa; R. Azuma; M. Mori; Y. Imasato; G.E. Allen; T. Tsujikawa; T. Tsukaguchi; C.R. Bradshaw; J. Jullien; K. Yamagata; K. Matsumoto; M. Anzai; H. Imai; J.B. Gurdon; M. Yamada
    The 50th annual meeting of Japan Society of Developmental Biologists  2017/05
  • HVJ Envelope Cell Fusion kit を用いた異属間体細胞核移植操作による再構築卵子の作製  [Not invited]
    東里香; 村井仁志; 小笠原里奈; 小木曽力; 鷲津朱理; 宮下実; 宮本圭; 細井美彦; 安齋政幸
    日本実験動物技術者協会関東支部第42回懇話会  2017/03
  • Nuclear actin in the regulation of transcription and nuclear structure  [Invited]
    Kei Miyamoto
    Human Frontier Science Program Kick-off Symposium  2017/01
  • 野生マウス由来線維芽細胞を用いた不活化ウイルス膜タンパク質による体細胞核移植法の開発  [Not invited]
    東里香; 村井仁志; 小橋朱里; 折杉卓哉; 小笠原里奈; 小木曽力; 鷲津朱理; 宮下実; 宮本圭; 細井美彦; 安齋政幸
    第39回日本分子生物学会年会  2016/12
  • Mechanisms of nuclear reprogramming in eggs and oocytes  [Invited]
    Kei Miyamoto
    King Abdullah University of Science and Technology  2016/11
  • Mechanisms of nuclear reprogramming in eggs and oocytes  [Invited]
    Kei Miyamoto
    New York University Abu Dhabi  2016/11
  • 体細胞の全能性獲得に関わる分子機構「生殖細胞のエピゲノムダイナミクスとその制御-ステムセルエイジングから解明する疾患原理.卵と初期発生」  [Invited]
    宮本圭
    新学術領域研究第4回公開シンポジウム  2016/11
  • 黒毛和種去勢牛の枝肉成績と関連する血清中バイオマーカー候補マイクロRNA の探索  [Not invited]
    松橋珠子; 池上春香; 森本 学; 永井宏平; 山口壮輝; 塚口智将; 樋口智香; 守田昂太郎; 宮本 圭; 坂口慎一; 松本和也
    関西畜産学会第66回大会  2016/10
  • 肥育期間中の黒毛和種牛の血清中バイオマーカー候補タンパク質の動態と出荷時の枝肉形質の相関性の検討  [Not invited]
    池上春香; 松橋珠子; 森本 学; 永井宏平; 山口壮輝; 塚口智将; 樋口智香; 守田昂太郎; 宮本 圭; 坂口慎一; 松本和也
    関西畜産学会第66回大会  2016/10
  • クローン動物がもたらした可能性「ノーベル賞の中の繁殖生物学」  [Invited]
    宮本圭
    第109回日本繁殖生物学会大会市民公開講座  2016/09
  • 受精卵の全能性を評価する新手法開発の取組み  [Not invited]
    塚口智将; Nicole Chung; 小林久人; 神尾明日香; 守田昂太郎; 樋口智香; 山口壮輝; 河野友宏; 松本和也; 宮本圭
    生物資源ゲノム解析拠点シンポジウム、東京農業大学  2016/09
  • The improved culture condition for mouse nuclear transfer embryos enables highly efficient nuclear reprogramming  [Invited]
    K. Miyamoto; Y. Tajima; K. Yoshida; T. Tsukaguchi; C.R. Bradshaw; G.E. Allen; M. Mori; Y. Imazato; J. Jullien; K. Matsumoto; H. Imai; J.B. Gurdon; M. Yamada
    The 3rd China-Japan-Korea reproduction meeting  2016/08
  • アフリカツメガエル卵母細胞を用いたリプログラミングとクロマチン動構造の解析  [Invited]
    宮本圭
    次世代両生類研究会第二回会合  2016/08
  • 受精卵の全能性を評価する新手法開発の取組み  [Not invited]
    塚口智将; Nicole Chung; 小林久人; 神尾明日香; 守田昂太郎; 樋口智香; 山口壮輝; 河野友宏; 松本和也; 宮本圭
    新学術領域「生殖細胞のエピゲノムダイナミクスとその制御」合同若手勉強会2016  2016/07
  • マウス2細胞期胚特異的レトロトランスポゾンの発現制御機構の解明に向けて  [Not invited]
    山口壮輝; 濱田歩花; 竹林真理愛; 守田昂太郎; 樋口智香; 塚口智将; 細井美彦; 山縣一夫; 松本和也; 宮本圭
    新学術領域「動的クロマチン構造と機能」クロマチン動構造ワークショップ  2016/07
  • Dynamic expression of PRMT5 and histone H3 symmetrically dimethylated at arginine 8 in mouse embryos during the 1-cell stage  [Not invited]
    Tomomasa Tsukaguchi; Kohtaro Morita; Chika Higuchi; Sho Uchibori; Tasuku Mitani; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming  2016/03
  • The role of Prdx in reducing H2O2 in pronuclei of mouse zygotes. International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming  [Not invited]
    Kohtaro Morita; Mikiko Tokoro; Chika Higuchi; Sho Uchibori; Tomomasa Tsukaguchi; Kohei Nagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming  2016/03
  • The ubiquitin-proteasome system which modulates the proper regulation of the onset of zygotic genome activation is involved in full term development of mice.  [Not invited]
    Chika Higuchi; Natsumi Shimizu; Kohtaro Morita; Sho Uchibori; Tomomasa Tsukaguchi; Kohei Nagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming  2016/03
  • Dynamic expression of PRMT5 and histone H3 symmetrically dimethylated at arginine 8 in mouse embryos during the 1-cell stage  [Not invited]
    Tomomasa Tsukaguchi; Kohtaro Morita; Chika Higuchi; Sho Uchibori; Tasuku Mitani; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on Epigenome Dynamics and Regulation in Germ Cells  2016/02
  • Peroxiredoxins reduce hydrogen peroxide in pronuclei of mouse zygotes  [Not invited]
    Kohtaro Morita; Mikiko Tokoro; Chika Higuchi; Sho Uchibori; Tomomasa Tsukaguchi; Kohei Nagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on Epigenome Dynamics and Regulation in Germ Cells  2016/02
  • The proper regulation of the onset of zygotic genome activation via ubiquitin-proteasome system is involved in full term development of mice  [Not invited]
    Chika Higuchi; Natsumi Shimizu; Kohtaro Morita; Sho Uchibori; Tomomasa Tsukaguchi; Kohei Nagai; Masayuki Anzai; Kazuo Yamagata; Yoshihiko Hosoi; Kei Miyamoto; Kazuya Matsumoto
    International Symposium on Epigenome Dynamics and Regulation in Germ Cells  2016/02
  • Reprogramming of somatic nuclei towards totipotency is greatly facilitated by small molecules through epigenetic alterations  [Invited]
    K. Miyamoto; Y. Tajima; K. Yoshida; M. Oikawa; T. Tsukaguchi; C.R. Bradshaw; G.E. Allen; J. Jullien; K. Matsumoto; H. Imai; J.B. Gurdon; M. Yamada
    International Symposium on Epigenome Dynamics and Regulation in Germ Cells  2016/02
  • 卵細胞における体細胞核の階層的クロマチン構造の変化と転写リプログラミングにおける役割  [Invited]
    宮本圭; ジェローム・ジュリアン; ビンセント・パスク; ジョージ・アレン; チャールズ・ブラッドシャー; ジョン・ガードン
    BMB2015  2015/12
  • PRMT5及びヒストンH3R8対称性ジメチル化は受精後の胚においてその局在が変化する  [Not invited]
    塚口智将; 守田昂太郎; 樋口智香; 内堀翔; 三谷匡; 細井美彦; 宮本圭; 松本和也
    第38回日本分子生物学会年会第88回日本生化学会大会合同大会  2015/12
  • Peroxiredoxinはマウス受精卵の核内で酸化ストレス軽減に関与している  [Not invited]
    守田昂太郎; 野老美紀子; 樋口智香; 内堀翔; 塚口智将; 永井宏平; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    第38回日本分子生物学会  2015/12
  • マウス初期胚においてユビキチン・プロテアソーム系は胚性ゲノム活性化の開始を制御し、その後の産仔への発生に関与する  [Not invited]
    樋口智香; 清水なつみ; 守田昂太郎; 内堀翔; 塚口智将; 永井宏平; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    第38回日本分子生物学会年会  2015/12
  • 還元型コエンザイムQ10はマウス体外老化卵母細胞において酸化的修飾タンパク質量の減少を導き、初期胚発生を改善する  [Not invited]
    内堀翔; 樋口智香; 守田昂太郎; 塚口智将; 安齋政幸; 山縣 一夫; 細井 美彦; 宮本 圭; 松本 和也
    第33回日本受精着床学会学術講演会  2015/11
  • 初期胚発生及びリプログラミングにともなうオープンクロマチン構造の変化と転写との関係  [Invited]
    宮本圭; G.E. Allen; C.R. Bradshaw; J.B. Gurdon
    第108回日本繁殖生物学会  2015/09
  • PRMT5及びヒストンH3R8対称性ジメチル化は受精後の胚においてその局在が変化する  [Not invited]
    塚口智将; 守田昂太郎; 樋口智香; 内堀翔; 三谷匡; 細井美彦; 宮本圭; 松本和也
    第108回日本繁殖生物学会大会  2015/09
  • コエンザイムQ10がマウス体外老化卵母細胞の受精とその後の発生に及ぼす影響  [Not invited]
    内堀翔; 樋口智香; 守田昂太郎; 塚口智将; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    第108回日本繁殖生物学会大会  2015/09
  • マウス前核期胚の核内においてPeroxiredoxin(Prdx)が過酸化水素の消去に関与する  [Not invited]
    守田昂太郎; 野老美紀子; 樋口智香; 内堀翔; 塚口智将; 永井宏平; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    第108回日本繁殖生物学会大会  2015/09
  • マウス胚におけるプロテアソーム系の一過性の阻害は胚性ゲノム活性化の開始を遅延し、産仔への発生を損なう  [Not invited]
    樋口智香; 清水なつみ; 守田昂太郎; 内堀翔; 塚口智将; 永井宏平; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也
    第108回日本繁殖生物学会大会  2015/09
  • アフリカツメガエル卵母細胞を用いたリプログラミング機構の解明  [Invited]
    宮本圭
    次世代両生類研究会第一回会合  2015/08
  • Genome-wide analysis of transcription-associated open chromatin regions during embryonic development and nuclear reprogramming  [Not invited]
    K. Miyamoto; G.E. Allen; C.R. Bradshaw; J.B. Gurdon
    International Symposium on Chromatin Structure, Dynamics and Function  2015/08
  • Molecular mechanisms how mouse somatic nuclei acquire totipotency  [Invited]
    Kei Miyamoto
    第4回発生工学研究センターセミナー  2015/08
  • Genome-wide analysis of transcription-associated open chromatin regions during early embryonic development and nuclear reprogramming  [Invited]
    Kei Miyamoto
    和歌山県立医科大学  2015/07
  • Mechanisms of transcriptional reprogramming in oocytes and eggs; nuclear actin and actin-binding proteins as key players  [Invited]
    宮本圭
    Protein Research (IPR) seminar “Nuclear organization and actin-dependent mechanisms in genome stability”,  2015/05
  • Genome-wide analysis of open chromatin regions during early embryonic development and nuclear reprogramming  [Invited]
    K. Miyamoto; G.E. Allen; C.R. Bradshaw; J.B. Gurdon
    第60回日本生殖医学会学術講演会  2015/04
  • Mechanisms of nuclear reprogramming in eggs and oocytes  [Invited]
    Kei Miyamoto
    The 78th of Stem Cell Biology and Regenerative Medicine Forum,  2015/03
  • Genome-wide analysis of open chromatin regions during early embryonic development and nuclear reprogramming  [Not invited]
    K. Miyamoto; G.E. Allen; C.R. Bradshaw; M. Teperek; J.B. Gurdon
    The 1st Gurdon Institute Post-doc Association symposium  2014/11
  • Mechanisms of transcriptional reprogramming in oocytes and eggs; nuclear actin and actin-binding proteins as key players  [Invited]
    Kei Miyamoto
    BPC seminar  2014/11
  • Nucleosome positioning during transcriptional reprogramming in oocytes  [Invited]
    K. Miyamoto; G.E. Allen; C.R Bradshaw
    The 87th Annual Meeting of the Japanese Biochemical Society (日本生化学学会),  2014/10
  • Mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    The 123rd RIKEN BRC seminar  2014/10
  • Mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    九州大学  2014/10
  • Transcriptional Reprogramming of Sperm and Somatic Nuclei in Xenopus Laevis Oocytes and Eggs  [Invited]
    Kei Miyamoto
    The 8th NIBB International Practical Course and The 3rd NIBB-TLL-DBS/NUS Joint International Practical Course  2014/09
  • Reprogramming of sperm and somatic nuclei in oocytes and eggs  [Invited]
    宮本圭
    FIRM (Future Investigators of Regenerative Medicine) Symposium,  2014/09
  • Transcriptional reprogramming of mammalian nuclei by maternal factors  [Invited]
    Kei Miyamoto
    King’s College London  2014/04
  • Transcriptional reprogramming of mammalian nuclei by maternal factors  [Invited]
    Kei Miyamoto
    Centre for Genomic Regulation (CRG)  2014/02
  • Transcriptional reprogramming of mammalian nuclei by maternal factors  [Invited]
    Kei Miyamoto
    Hiroshima University  2013/12
  • Transcriptional reprogramming of mammalian nuclei by maternal factors  [Invited]
    Kei Miyamoto
    Nagoya University  2013/12
  • Transcriptional reprogramming of mammalian nuclei by maternal factors  [Invited]
    Kei Miyamoto
    Chromatin Dynamics Symposium  2013/12
  • Nuclear actin and actin-binding proteins in nuclear reprogramming and embryonic development: their important roles in transcriptional regulation  [Invited]
    Kei Miyamoto
    The 36th Annual of the Molecular Biology Society of Japan  2013/12
  • Nuclear actin and nuclear actin-binding protein as new players in embryonic development and reprogramming  [Invited]
    宮本圭
    Developmental Biology Seminar Series, University of Cambridge  2013/11
  • Mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    RIKEN  2012/12
  • The mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    Experimental Animals  2012/06
  • Nuclear WAVE1 is necessary for transcriptional reprogramming by Xenopus eggs and oocytes  [Not invited]
    K. Miyamoto; J.B. Gurdon
    International Society for Stem Cell Research (ISSCR) Meeting  2012/06
  • Mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    Stem Cells & Bioprocessing Europe  2012/06
  • WAVE1 is required for transcriptional reprogramming by Xenopus eggs and oocytes  [Not invited]
    K. Miyamoto; J.B. Gurdon
    British Society for Cell Biology (BSCB), British Society for Developmental Biology (BSDB) and Japanese Society for Developmental Biologists (JSDB) Joint Spring Meeting  2012/04
  • Mechanisms of nuclear reprogramming by eggs and oocytes  [Invited]
    Kei Miyamoto
    Tohoku University  2011/12
  • Nuclear actin in transcriptional reprogramming by Xenopus laevis oocytes  [Invited]
    Kei Miyamoto
    The WGF symposium “Actin and actin-associated proteins from gene to polysomes”  2011/09
  • Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes  [Not invited]
    K. Miyamoto; V. Pasque; J. Jullien; J.B. Gurdon
    1st annual Cambridge Stem Cell international symposium: Pluripotency and Development  2011/07
  • Identification of reprogramming factors in oocytes by a novel screening method  [Not invited]
    K. Miyamoto; J.B. Gurdon
    BSCB and BSDB Joint Spring Meeting  2010/04
  • Establishment of novel systems for unraveling reprogramming  [Invited]
    Kei Miyamoto
    Kinki University  2010/02
  • Reprogramming in the oocyte cell-free system  [Invited]
    Kei Miyamoto
    Kinki University  2009/04
  • Mammalian oocyte extracts induce chromatin remodeling and dedifferentiation of somatic cells, but do not global demethylation  [Not invited]
    K. Miyamoto; T. Tsukiyama; N. Minami; M. Yamada; H. Imai
    International Congress on Animal Reproduction (ICAR)  2008/07
  • Reprogramming of somatic cells in cell-free extracts from mammalian oocytes and identification of reprogramming-related proteins  [Invited]
    Kei Miyamoto
    New Bolton Center; University of Pennsylvania  2008/06
  • Reversible permeabilization of mammalian somatic cells treated with digitonin for reprogramming and dedifferentiation by Xenopus cell-free system  [Not invited]
    K. Miyamoto; T. Yamashita; N. Minami; M. Yamada; H. Imai
    ISSCR  2008/06
  • DNA demethylation associated with nuclear reprogramming of the somatic cell genome in cell-free extracts from mammalian oocytes  [Not invited]
    K. Miyamoto; T. Tsukiyama; Y. Yang; N. Li; N. Minami; M. Yamada; H. Imai
    Society for the Study of Reproduction (SSR)  2008/05
  • Differential nuclear reprogramming of somatic cells by porcine germinal vesicle and metaphase II oocyte extracts  [Not invited]
    K. Miyamoto; T. Tsukiyama; N. Minami; M. Yamada; H. Imai
    Asian Reproductive Biotechnology Society (ARBS)  2007/11
  • Nuclear reprogramming of porcine cells and their use as donor cell for nuclear transfer after treatment in Xenopus egg extracts  [Not invited]
    K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai
    The International Embryo Transfer Society (IETS) Meeting  2007/01
  • Reprogramming of intact and permeabilized mammalian somatic cells by Xenopus egg extract  [Not invited]
    K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai
    ARBS  2006/11
  • Nuclear reprogramming of porcine fibroblast cells by Xenopus egg extracts  [Not invited]
    K. Miyamoto; Y. Nagao; N. Minami; M. Yamada; K. Ohsumi; H. Imai
    IETS Meeting  2006/01
  • Imai. Reprogramming events of porcine fibroblast cells in Xenopus egg extracts  [Not invited]
    K. Miyamoto; T. Furusawa; T. Tokunaga; Y. Nagao; N. Minami; M. Yamada; K. Ohsumi; H. Imai
    International Symposium on Germ Cells, Epigenetics, Reprogramming and Embryonic Stem Cells  2005/11
  • Cell cycle synchronization of donor cells at G1 phase and developmental ability of nuclear transfer embryos in miniature pigs  [Not invited]
    K. Miyamoto; Y. Hoshino; Y. Nagao; N. Minami; M. Yamada; H. Imai
    IETS Meeting  2005

MISC

  • 受胎率向上に向けた早期胚質評価
    宮本圭  THE NAITO FOUNDATION REPORT 内藤財団時報 第112号  (112)  1  -135  2023/09  [Invited]
  • 生殖補助医療の発展にむけた着床前胚発生過程の理解
    井上明裕; 宮本圭  Medical Science Digest 9月号  49-  (10)  36  -39  2023/09  [Invited]
  • 体外成熟培養に供試したヒト卵のトランスクリプトーム解析
    山本 真理; 武内 大輝; 福井 愛実; 前沢 忠志; 西岡 美喜子; 池田 智明; 松本 和也; 宮本 圭  日本生殖医学会雑誌  67-  (3)  134  -134  2022/07
  • Dialogue between Young Leaders and Nobel Laureates
    Kei Miyamoto  18th Annual Meeting of STS forum  2021/09  [Invited]
  • 卵子の秘めた力に魅せられて
    宮本圭  日本学士院ニュースレター  27-  9  2021/04
  • 宮本圭; 武内大輝; 武内大輝; 山本真理; 福井愛実; 前沢忠志; 前沢忠志; 西岡美喜子; 西岡美喜子; 西岡美喜子; 池田智明; 池田智明; 池田智明; 松本和也  日本生殖医学会雑誌  66-  (4)  2021
  • Nuclear reprogramming:From one cell type to another
    宮本圭  Research Outreach  (109)  14  -17  2019/08  [Invited]
  • 坂本裕子; 奥野智美; LI Yang; 山本真理; 神谷拓磨; 越智浩介; 井橋俊哉; 辻本佳加理; 松橋珠子; 松本和也; GROSSE Robert; 宮本圭  Journal of Mammalian Ova Research  36-  (1)  S49  2019/04
  • 永井宏平; 宮本裕史; 安齋政幸; 東里香; 西端智也; 山縣一夫; 加藤博己; 宮本圭; KOLODEZNIKOV Igor I.; PROTOPOPOV Albert V.; PLOTNIKOV Valerii V.; 細井美彦; 三谷匡; 松本和也; 入谷明  日本プロテオーム学会大会プログラム・抄録集  2019-  2019
  • 西端智也; 永井宏平; 山縣一夫; 宮本裕史; 安齋政幸; 加藤博己; 宮本圭; 東里香; KOLODEZNIKOV Igor I.; PROTOPOPOV Albert V.; PLOTNIKOV Valerii V.; 細井美彦; 三谷匡; 松本和也; 入谷明  日本プロテオーム学会大会プログラム・抄録集  2019-  2019
  • 守田昂太郎; 樋口智香; 安齋政幸; 松橋珠子; 黒坂哲; 加藤博己; 三谷匡; 山縣一夫; 細井美彦; 宮本圭; 松本和也  日本実験動物学会総会講演要旨集(Web)  66th-  2019
  • IHASHI Shunya; OCHI Kousuke; SAKAMOTO Yuko; TSUJIMOTO Kagari; KASAHARA Yoshito; MATSUHASHI Tamako; MATSUMOTO Kazuya; MIYAMOTO Kei; MORI Miki; IMASATO Yuma; KUSAKABE Haruna; KAJIKURI Naoaki; MATSUZAWA Yuka; KAMIYA Takuma; OKUNO Tomomi; YAMAMOTO Mari  The Journal of Reproduction and Development Supplement  112-  (0)  P  -98-P-98  2019
  • 奥野智美; 樋口智香; 神谷拓磨; 山本真理; 越智浩介; 西野亜理紗; 井橋俊哉; 辻本佳加理; 松橋珠子; 安齋政幸; 黒坂哲; 三谷匡; 山縣一夫; 細井美彦; 松本和也; 宮本圭  Journal of Reproduction and Development  64-  (Suppl Japanese Issue)  j125  -70-P-70  2018/09
  • 神谷拓磨; 本上遥; 久米健太; 樋口智香; 奥野智美; 山本真理; 越智浩介; 井橋俊哉; 辻本佳加理; 松橋珠子; 細井美彦; 松本和也; 宮本圭  Journal of Reproduction and Development  64-  (Suppl Japanese Issue)  j117  -54-P-54  2018/09
  • 松橋珠子; 池上春香; 越智浩介; 大林賢伍; 佐野文美; 森隆史; 本廣多胤; 奥野智美; 神谷拓磨; 永井宏平; 宮本圭; 吉廣卓哉; 坂口慎一; 松本和也  日本畜産学会大会講演要旨  124th-  184  2018/03
  • 奥野智美; 山口壮輝; 樋口智香; 神谷拓磨; 山本真理; 越智浩介; 西野亜理紗; 井橋俊哉; 辻本佳加理; 坂本裕子; 松橋珠子; 安齋政幸; 黒坂哲; 三谷匡; 山縣一夫; 細井美彦; 松本和也; 宮本圭  日本分子生物学会年会プログラム・要旨集(Web)  41st-  ROMBUNNO.2P‐0413 (WEB ONLY)  2018
  • 核アクチンのマウス初期胚発生における役割
    守田昂太郎; 鷹巣篤志; 波多野裕; 簾新智; プレッスナー マティアス; 松本和也; 山縣一夫; グロッセ ロバート; 宮本圭  第40回日本分子生物学会  2017/12
  • 動物におけるリプログラミング現象の解明から、細胞初期化の要素を特定する〜
    宮本圭  Top Researchers  2017/09  [Invited]
  • Efficient nuclear reprogramming of somatic cells towards totipotency is supported by synergistic effects of small molecules.
    K. Miyamoto; Y. Tajima; K. Yoshida; M. Oikawa; R. Azuma; M. Mori; Y. Imasato; G.E. Allen; T. Tsujikawa; T. Tsukaguchi; C.R. Bradshaw; J. Jullien; K. Yamagata; K. Matsumoto; M. Anzai; H. Imai; J.B. Gurdon; M. Yamada  the 50th annual meeting of Japan Society of Developmental Biologists  2017/05  [Refereed][Invited]
  • 樋口智香; 守田昂太郎; 山口壮輝; 松橋珠子; 永井宏平; 安齋政幸; 安齋政幸; 山縣一夫; 細井美彦; 宮本圭; 松本和也  日本卵子学会誌  2-  (1)  S48  -S48  2017/04
  • 守田昂太郎; 樋口智香; 山口壮輝; 松橋珠子; 松橋珠子; 永井宏平; 安齋政幸; 安齋政幸; 加藤博巳; 加藤博巳; 山縣一夫; 細井美彦; 宮本圭; 松本和也  日本卵子学会誌  2-  (1)  S47  -S47  2017/04
  • The improved culture condition for mouse nuclear transfer embryos enables highly efficient nuclear reprogramming.
    K. Miyamoto; Y. Tajima; K. Yoshida; T. Tsukaguchi; C.R. Bradshaw; G.E. Allen; M. Mori; Y. Imazato; J. Jullien; K. Matsumoto; H. Imai; J.B. Gurdon; M. Yamada  The 3rd China-Japan-Korea reproduction meeting  2016/08  [Refereed][Invited]
  • Reprogramming of somatic nuclei towards totipotency is greatly facilitated by small molecules through epigenetic alterations
    Kei Miyamoto; Tomomasa Tsukaguchi; Charles R Bradshaw; George E Allen; Jerome Jullien; Kazuya Matsumoto; J. B. Gurdon; Masayasu Yamada  International symposium on epigenome dynamics and regulation in germ cells  2016/02  [Refereed][Invited]
  • HIGUCHI Chika; SHIMIZU Natsumi; MORITA Kohtaro; UCHIBORI Sho; TSUKAGUCHI Tomomasa; NAGAI Kohei; ANZAI Masayuki; YAMAGATA Kazuo; HOSOI Yoshihiko; MIYAMOTO Kei; MATSUMOTO Kazuya  The Journal of Reproduction and Development Supplement  108-  (0)  P  -12-P-12  2015
  • MORITA Kohtaro; TOKORO Mikiko; HIGUCHI Chika; UCHIBORI Sho; TSUKAGUCHI Tomomasa; NAGAI Kohei; ANZAI Masayuki; YAMAGATA Kazuo; HOSOI Yoshihiko; MIYAMOTO Kei; MATSUMOTO Kazuya  The Journal of Reproduction and Development Supplement  108-  (0)  P  -15-P-15  2015
  • UCHIBORI Sho; HIGUCHI Chika; MORITA Kohtaro; TSUKAGUCHI Tomomasa; ANZAI Masayuki; YAMAGATA Kazuo; HOSOI Yoshihiko; MIYAMOTO Kei; MATSUMOTO Kazuya  The Journal of Reproduction and Development Supplement  108-  (0)  P  -10-P-10  2015
  • 卵子および卵母細胞に特異的なタンパク質は移植された体細胞核に階層的にはたらきかけ急速かつゲノムワイドな転写のリプログラミングを誘導する
    宮本 圭; Jerome Jullien・Vincent Pasque・John; B. Gurdon  ライフサイエンス新着論文レビュー(ライフサイエンス統合データベースセンター)  2014/08  [Refereed][Invited]
  • 核内Wave1は卵母細胞における転写の初期化と正常な発生に必要である
    Kei Miyamoto  Japanese Scientist in Science 2013  60  -61  2014  [Refereed][Invited]
  • アクチン結合タンパク質Wave1は卵母細胞の核における転写プログラムの初期化と正常な発生に必要である
    宮本圭  ライフサイエンス新着論文レビュー(ライフサイエンス統合データベースセンター)  2013/09  [Refereed][Invited]
  • The roles of Hsp90 in reprogramming process in mouse preimplantation embryonic development
    Luong PQ; Matsumoto M; Suzuki S; Kitamura N; Miyamoto K; Minami N; Yamada M; Imai H  CiRA International Symposium  2012  [Refereed]
  • Differential nuclear reprogramming of somatic cells by porcine germinal vesicle and metaphase II oocyte extracts.
    Miyamoto K; Tsukiyama T; Minami N; Yamada M; Imai H  The 4th Asian Reproductive Biotechnology Conference  2007  [Refereed]
  • 「Xenopus卵抽出液による哺乳類細胞のリプログラミング
    宮本 圭; 古澤 軌; Goel Sandeep; 南 直次郎; 山田 雅保; 徳永 智之; 大隅 圭太; 今井 裕  日本畜産学会第105回大会 九州大学  2006/03
  • ドナー細胞の細胞周期制御とミニブタ核移植胚の発生能:G1期あるいはG0/G1期への細胞周期同期化による影響
    宮本 圭; 星野 洋一郎; 長尾 恭光; 南 直次郎; 山田 雅康; 今井 裕  日本畜産学会第104回大会、東京大学  2006/03  [Refereed]
  • Reprogramming of intact and permeabilized mammalian somatic cells by Xenopus egg extract
    Miyamoto K; Ohnuki M; Minami N; Yamada M; Imai H  The 3rd Asian Reproductive Biotechnology Conference  2006  [Refereed]

Awards & Honors

  • 2021/04 近畿大学 研究奨励褒賞
     
    受賞者: 宮本圭
  • 2021/01 Japan Academy Japan Academy Medal
     卵内初期化機構に関する研究 
    受賞者: Kei Miyamoto
  • 2020/12 Japan Society for the Promotion of Science (JSPS) JSPS prize
     Research on Mechanisms of Nuclear Reprogramming in Eggs and Oocytes 
    受賞者: Kei Miyamoto
  • 2018/09 Society for Reproduction and Development SRD Young Investigator Award
     卵内リプログラミング機構に関する研究 
    受賞者: Kei Miyamoto
  • 2018/04 The Minister of Education, Culture, Sports, Science and Technology Young Scientists’ Prize
     卵内初期化機構に関する研究 
    受賞者: 宮本圭
  • 2016/07 Human Frontier Science Program Human Frontier Project Grant
     Nuclear actin assembly in chromatin structure and dynamics for cell cycle control and reprogramming 
    受賞者: Kei Miyamoto
  • 2012/04 Herchel Smith Postdoctoral Fellowship award
     
    受賞者: Kei Miyamoto
  • 2007/11 Asian Reproductive Biotechnology Society (ARBS), Asian Reproductive Biotechnology Society (ARBS) 「First Prize, Outstanding Presentation Award」
     
    受賞者: 宮本圭
  • 2006/12 Asian Reproductive Biotechnology Society (ARBS) 「Outstanding Presentation Award.」
     
    受賞者: 宮本圭
  • 2006/03 第106回日本畜産学会 優秀発表賞
     
    受賞者: 宮本圭
  • 2006/01 The International Embryo Transfer Society (IETS) Meeting 「Student Research Competition Finalist」
     
    受賞者: 宮本圭

Research Grants & Projects

  • 核内アクチンタンパク質の生物学的意義の解明への助成
    公益財団法人 武田科学振興財団:2022年度 武田科学振興財団 ライフサイエンス研究継続助成
    Date (from‐to) : 2022/09 -2027/05 
    Author : 宮本圭
  • 日本学術振興会:科学研究費助成事業 基盤研究(B)
    Date (from‐to) : 2023/04 -2027/03 
    Author : 宮本 圭
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)
    Date (from‐to) : 2022/06 -2026/03 
    Author : 島本 勇太; 岩城 光宏; 宮本 圭
  • 母性転写物量を指標とした早期胚質評価法の確立
    公益財団法人 内藤記念科学振興財団:第54回(2022年度) 内藤記念科学奨励金・研究助成
    Date (from‐to) : 2022/12 -2024/09 
    Author : 宮本圭
  • マウス初期胚核の物理特性と核骨格タンパク質の関係性の解明
    Date (from‐to) : 2023/04 -2024/03 
    Author : 宮本 圭; 島本 勇太
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    Date (from‐to) : 2021/04 -2024/03 
    Author : 田村 功; 宮本 圭
  • 科学研究費助成事業(科学研究費補助金):新学術領域研究(研究領域提案型)
    Date (from‐to) : 2019/07 -2024/03 
    Author : 宮本圭
  • マウス初期胚核の物理 特性と核骨格タンパク 質の関係性の解明
    2022年度国立遺伝学研究所共同研究【共同研究(B)】
    Date (from‐to) : 2022/04 -2023/03 
    Author : 宮本圭; 島本 勇太
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
    Date (from‐to) : 2020/07 -2022/03 
    Author : MIYAMOTO KEI
     
    In the field of livestock reproduction, improving the conception rate of in vitro produced bovine embryos is an urgent issue. The low fertility is presumably linked to deterioration of embryo quality. In this study, we focused on maternal transcripts in zygotes, and tried to identify the transcripts related to embryo quality in order to establish a system to predict the developmental ability of fertilized bovine embryos by taking advantage of the expression levels of those maternal transcripts. Transcriptomes of zygotes and their sibling polar bodies were examined and gene expression was compared between zygotes with high developmental abilities and those with low abilities. We then identified transcripts whose expression was specifically altered in zygotes with high developmental potentials. Furthermore, we have shown a system for predicting bovine zygotes with high developmental abilities and good genetic traits by examining expression and sequence information of the transcripts.
  • 核内アクチンタンパク質の生物学的意義の解明
    公益財団法人 武田科学振興財団:ライフサイエンス研究助成
    Date (from‐to) : 2019/09 -2021/09 
    Author : 宮本圭
  • 転写解析特化型核移植系を用いたリプログラミング因子の同定
    文部科学省科学研究費助成事業:若手研究(A)
    Date (from‐to) : 2017/04 -2021/03 
    Author : 宮本圭
  • 非侵襲的に高発生能の受精卵を選抜する手法の開発
    公益財団法人 内藤記念科学振興財団:第50回(2018年度) 内藤記念科学奨励金・研究助成
    Date (from‐to) : 2018/12 -2020/09 
    Author : 宮本圭
  • 転写リプログラミングにおけるクロマチン構造変化の階層的理解
    科学研究費助成事業(科学研究費補助金):新学術領域研究
    Date (from‐to) : 2019/04 -2019/06 
    Author : 宮本圭
  • Human Frontier Science Program:ヒューマン・フロンティア・サイエンス・プログラム
    Date (from‐to) : 2016/07 -2019/06 
    Author : 宮本圭
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research
    Date (from‐to) : 2016/04 -2019/03 
    Author : Minami Naojiro
     
    During mouse preimplantation development, a new developmental program starts through chromatin remodeling. Histone H4 Lysine 20 (H4K20) monomethylation has been reported to control gene expression and DNA damage. However, little is known about the role of methylation by SETD8 in mouse preimplantation development. In the present study, we revealed that SETD8 regulated cell cycle progression during mouse preimplantation development. Although it is known that SETD8 methylates not only H4K20 but also non-histone proteins such as p53 to control its function, we found that developmental arrest of SETD8-inhibited embryos was due to inhibition of H4K20 monomethylation. In addition, development delay caused by transient inhibition of SETD8 was rescued by inhibition of ATR pathway, which play crucial role in DNA damage repairment and cell cycle checkpoint. These results suggest that SETD8-mediated H4K20 monomethylation regulate cell cycle progression in mouse preimplantation development.
  • 時空間的核アクチン重合化制御によるクロマチン構造と転写状態の変動
    文部科学省科学研究費助成事業:新学術領域研究
    Date (from‐to) : 2016/04 -2018/03 
    Author : 宮本圭
  • 体細胞核の全能性獲得に関る分子機構
    文部科学省科学研究費助成事業:新学術領域研究
    Date (from‐to) : 2016/04 -2018/03 
    Author : 宮本圭
  • 細胞分裂に依存しない新規リプログラミング系の構築
    公益財団法人 住友財団:奨学寄附金
    Date (from‐to) : 2015/09 -2017/03 
    Author : 宮本圭
  • マウス胚を用いた細胞分裂非依存的リプログラミング系の構築
    文部科学省科学研究費助成事業:研究活動スタート支援
    Date (from‐to) : 2015/09 -2016/03 
    Author : 宮本圭
  • Herchel Smith Postdoctoral Fellowship Research Grant
    Date (from‐to) : 2012/04 -2015/03 
    Author : 宮本圭
  • collaboration work with Central Institute for Experimental Animals on marmoset
    The Great Britain Sasakawa Foundation Grant
    Date (from‐to) : 2012/12 -2013/04 
    Author : Kei Miyamoto
  • The Grant from the Japan Society for the Promotion of Science
    Research Fellowship Program
    Date (from‐to) : 2007/04 -2009/03 
    Author : Kei Miyamoto

Media Coverage

  • Date : 2022/01/13
    Publisher, broadcasting station: Japan Science and Technology Agency
    Program, newspaper magazine: Science Japan
    Internet
  • Date : 2021/11/25
    Writer: Other than myself
    Publisher, broadcasting station: 国立研究開発法人科学技術振興機構
    Program, newspaper magazine: JST客観日本(中国版)
    科学研究(生物医学) Internet
  • Date : 2021/02/26
    Publisher, broadcasting station: United States of America
    Program, newspaper magazine: FOX News Channel
    Media report
  • 宮本氏(岡山出身)学士院奨励賞
    Date : 2021/01/13
    Program, newspaper magazine: 山陽新聞朝刊
    4面 Paper
  • 学士院、6人に学術奨励賞
    Date : 2021/01/13
    Program, newspaper magazine: 朝日新聞デジタル
    Paper
  • 学士院奨励賞 浦川氏ら6人
    Date : 2021/01/13
    Program, newspaper magazine: 京都新聞
    5面 Paper
  • 学士院、学術奨励賞に6氏
    Date : 2021/01/13
    Program, newspaper magazine: 朝日新聞
    27面 Paper
  • 学士院 学術奨励賞に6人
    Date : 2021/01/13
    Program, newspaper magazine: 読売新聞
    31面 Paper
  • 受精卵に重合化アクチン 近大のグループ発見
    Date : 2020/07/29
    Program, newspaper magazine: 毎日新聞大阪版
    P23 Paper
  • 受精卵の核内構造を発見 近畿大 生殖医療などに貢献期待
    Date : 2020/07/14
    Program, newspaper magazine: 和歌山新報
    1面 Paper
  • 世界初!命の始まりである受精卵に新たな核構造を発見 動物発生の謎に迫る研究成果
    Date : 2020/07/01
    Newscast Internet
  • 受精卵の細胞核の研究で成果
    Date : 2020/07/01
    Publisher, broadcasting station: テレビ和歌山
    Program, newspaper magazine: WTVニュース
    Media report
  • 受精卵から動物発生 アクチン重合化 重要
    Date : 2020/07/01
    Program, newspaper magazine: 日刊工業新聞
    科学技術・大学 25面 Paper
  • 受精卵が成体になる過程で重要なたんぱく質 近大グループ確認
    Date : 2020/07/01
    Program, newspaper magazine: 毎日新聞デジタル
    Paper
  • マンモス化石の細胞核「死んでいなかった」シベリアで発掘 近大、「復活」に前進
    Date : 2019/03/12
    Program, newspaper magazine: 産経新聞
    社会面 30面 Paper
  • マンモス「復活」へ前進 細胞の核 死んでいなかった」近畿大チーム発表
    Date : 2019/03/12
    Program, newspaper magazine: 【関東版】産経新聞
    社会面 24面 Paper
  • マンモス細胞核動いた 近大などのチーム確認
    Date : 2019/03/12
    Program, newspaper magazine: 朝日新聞
    3面 Paper
  • マンモス復活へ前進? 2.8万年前の細胞核 動き確認
    Date : 2019/03/12
    Program, newspaper magazine: 【関東版】朝日新聞
    社会面 33面 Paper
  • マンモス細胞核目覚めた マウス卵子内 分裂直前の状態に 近大など 永久凍土から発掘
    Date : 2019/03/12
    Program, newspaper magazine: 読売新聞
    37面 Paper
  • マンモス細胞核目覚めた マウス卵子内で変化
    Date : 2019/03/12
    Program, newspaper magazine: 【関東版】読売新聞
    社会面 37面 Paper
  • マンモスの細胞核再生 近畿大クローンで復活に前進
    Date : 2019/03/12
    Program, newspaper magazine: 日本経済新聞
    社会面 38面 Paper
  • マンモス復活へ一歩?細胞分裂の兆し確認
    Date : 2019/03/12
    Program, newspaper magazine: 毎日新聞
    社会面 29面 Paper
  • マンモス細胞 初期の動き 近畿大など マウス卵子に核移植
    Date : 2019/03/12
    Program, newspaper magazine: 【関東版】毎日新聞
    総合・社会面 25面 Paper
  • マンモス復活へ前進 細胞核に変化 近大チーム発表
    Date : 2019/03/12
    Program, newspaper magazine: 【関東版】東京新聞
    朝刊 総合面 2面 Paper
  • マンモスの遺伝子 “活動する力を保っていた” 近畿大など発表
    Date : 2019/03/11
    サイカル(SCIENCE&CULTURE) journal by NHK Internet
  • カギは遺伝子密度 細胞の初期化 再生医療 発展に期待 近畿大など発見
    Date : 2018/07/11
    Program, newspaper magazine: 日刊工業新聞
    24面 Paper
  • アクチンタンパク質が細胞核形成の重要因子
    Date : 2017/12/01
    Program, newspaper magazine: 科学新聞
    8面 Paper
  • クローンマウスの出生率向上について
    Date : 2017/04/28
    Program, newspaper magazine: フジサンケイビジネスアイ・西日本
    19面 Paper
  • クローンマウスの作製効率向上について
    Date : 2017/04/19
    Program, newspaper magazine: 日経産業新聞
    9面 Paper
  • 体細胞クローンマウスの発生率向上について
    Date : 2017/04/17
    Program, newspaper magazine: 日刊工業新聞
    17面 Paper
  • クローンマウスの作製効率向上について
    Date : 2017/04/17
    Program, newspaper magazine: 日本經濟新聞(夕刊)
    12面 Paper
  • クローンマウスの作製効率向上について
    Date : 2017/04/17
    Program, newspaper magazine: 化学工業日報
    16面 Paper
  • クローンマウスの作製効率向上について
    Date : 2017/04/16
    Program, newspaper magazine: 東奥日報社
    16面 Paper
  • クローンマウスの作製効率向上について
    Date : 2017/04/16
    Program, newspaper magazine: 四国新聞
    3面 Paper
  • 遺伝子改変の効率アップについて
    Date : 2015/11/19
    Program, newspaper magazine: 読売新聞
    37面 Paper

Others

  • 2018/02 -2018/02  「世界をリードする最先端の分子発生工学研究室」 
    週刊 東洋経済 第6772号 2018年2月3日発行 P111


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