KAJIYAMA Shinichiro

Researcher Information

Degree

• 1（Kyoto University）

URL

https://www.kindai.ac.jp/bost/

J-Global ID

• 200901072334301612

Research Interests

• 非食用油脂生産植物   代謝産物分析   レーザー細胞操作   植物細胞工学   post genome science   metabolite analysis   non-linear photonics   laser cell manipulation   Cell enginerring   

Research Areas

• Life sciences / Bioorganic chemistry
• Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering
• Nanotechnology/Materials / Biochemistry
• Nanotechnology/Materials / Green/sustainable/environmental chemistry
• Nanotechnology/Materials / Analytical chemistry
• Environmental science/Agricultural science / Environmental materials/recycling technology
• Environmental science/Agricultural science / Environmental load reduction/restoration technology
• Environmental science/Agricultural science / Environmental policy and society
• Environmental science/Agricultural science / Environmental impact assessment

Academic & Professional Experience

• 2011/04 - Today  Kinki Univ.School of Bio-oriented Science and technologyProfessor
• 2008 - 2011/03  Kinki Univ.School of Bio-oriented Science and technologyAssociate professor
• 2005 - 2008  Osaka Univ.Grad. school of EngineeringInvited Associate Professor
• 1995 - 2005  Osaka Univ.Faculty of EngineeringAssintant Professor
• 1991 - 1995  Okayama Univ.Faculty of AgricultureResearch Assistant

Education

• 1989/04 - 1991/03  Kyoto University  農学研究科  食品工学専攻
• 1985/04 - 1989/03  Kyoto University  Faculty of Agriculture  食品工学科
•        - 1989  Kyoto University  Faculty of Agriculture

Association Memberships

• アメリカ化学会（ACS)   日本生物工学会   日本化学会   日本農芸化学会   ACS   

Published Papers

• Volatile profiling of fruits of 17 mango cultivars by HS-SPME-GC/MS combined with principal component analysis

  Kosuke Shimizu; Tetsuya Matsukawa; Risa Kanematsu; Kimihisa Itoh; Shinya Kanzaki; Shigeru Shigeoka; Shin'ichiro Kajiyama

  Bioscience, Biotechnology, and Biochemistry Oxford University Press (OUP) 85 (8) 1789 - 1797 0916-8451 2021/07 [Refereed]
   

  Headspace solid-phase microextraction combined with gas chromatography/mass spectrometry is one of the strongest tools for comprehensive analysis of volatile compounds and has been used to analyze aromatic components of mango and investigate its varietal characteristics. In this study, profiling of aroma compounds in 17 mango cultivars, grown in the same green house to exclude the effect of environmental factors, was conducted and the patterns were subjected to principal component analysis (PCA) to identify the relationship between the aroma components and cultivars. Fifty-nine different volatile constituents were detected from the blends of these 17 mango cultivars. The cultivars were divided into 4 clusters using PCA based on the volatile components determined in the study. Aiko was found to mainly contain δ-3-carene and showed a composition more similar to its pollen parent, Irwin, than to its seed parent, Chiin Hwang No. 1.
• Effective Utilization of Citrus unshiu Plant Waste Extracts with Lipase Inhibitory Activities

  Kimihisa Itoh; Tetsuya Matsukawa; Takahiro Deguchi; Momona Yamagami; Norimichi Tomohiro; Kazuya Murata; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka

  Journal of Plant Studies Canadian Center of Science and Education 10 (2) 1 - 7 1927-050X 2021/04 [Refereed]
   

  This study examines the effect of quantity sold (sales volume) on profitability of market participating smallholder farmers in northern Ghana. Market participation has been shown to be important for increasing incomes and improving production efficiency for farm households but still remains low in SSA. While agribusiness and development experts generally advocate for more intensive market participation, it is not clear if selling more results in more profits for smallholder farmers in remote markets that are prone to exorbitant transaction costs. The data used in this study is from the APS survey conducted in 2013 and 2014 in Northern Ghana which had a sample size of 527. The study is based on the theory of profit maximization, in which separability is inferred from observed market participation. OLS regression is used for empirical estimation after rejecting the hypothesis of endogeneity in the model. Mean gross margin/ kg across four groups of farmers ranked by quantity sold is also statistically examined. The results confirm the existence of economies of scale and also show that different crops have different effects on profitability. The results also show that although unambiguously positive, the relationship between quantity sold and profitability may not be linear.
• Effective Utilization of Citrus unshiu Plant Waste Extracts with Lipase Inhibitory Activities

  Kimihisa Itoh; Tetsuya Matsukawa; Takahiro Deguchi; Momona Yamagami; Norimichi Tomohiro; Kazuya Murata; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka

  Journal of Plant Studies Canadian Center of Science and Education 10 (2) 1 - 1 1927-0461 2021/04 [Refereed]
   

  During the course of Citrus unshiu fruit cultivation, large amounts of plant material including pruned leaves, thinned-out flowers and unripe fruit are obtained; these materials are generally considered unusable and discarded as plant wastes. We have previously attempted to establish an effective use of such plant wastes as beneficial natural resources and found that a methanolic extract of pruned Citrus leaves (CUL-ext) exhibited inhibitory activity against porcine pancreatic lipase. In this study, we sought to identify further the effective uses of C. unshiu plant wastes by determining the lipase inhibitory activity of methanolic extracts of thinning out flowers (CUF-ext) and unripe fruit (CUUF-ext). We accordingly found that the inhibitory activity of CUF-ext was superior to that of CUUF-ext and comparable to that previously observed using CUL-ext. Fractionation of CUF-ext and CUUF-ext, followed by chromatographic analyses, revealed that the pancreatic lipase inhibitory activities of these extracts could be attributed, at least in part, to the flavonoids hesperidin, nobiletin, narirutin and rutin. On the basis of present findings, we propose that, in addition to pruned Citrus leaves, the thinned-out flowers and unripe fruit of C. unshiu are natural resources which are suitable for preparing constituents with lipase inhibitory activity.
• Ability of Ureaplasma parvum to invade mouse sperm, fertilize eggs through infected sperm, and impair mouse sperm function and embryo development

  Kazutoshi Ito; Kazuki Akai; Fumiko Nishiumi; Yukiko Nakura; Heng Ning Wu; Teru Kurata; Akira Onodera; Yuichi Kawai; Shinichiro Kajiyama; Itaru Yanagihara

  F&S Science Elsevier BV 2 (1) 13 - 23 2666-335X 2021/02 [Refereed]
   

  Objective: To examine the effect of Ureaplasma parvum (U. parvum) infection on mouse sperm motility, structure, and fertilizing ability and on embryo development. Design: In vitro model of the effects of U. parvum serovar 3 infection on mouse sperm. Setting: Basic research laboratory. Intervention(s): None. Animals: Mice. Main Outcome Measure(s): Mouse sperm motility was examined using the swim-up method, and their motility parameters were analyzed using the sperm motility analysis system. Localization and invasion of U. parvum were observed with fluorescence, confocal, and scanning electron microscopy. After in vitro fertilization with U. parvum–infected sperm, the quality of the fertilized egg and embryo development were assessed. Result(s): U. parvum was attached and internalized into mouse sperms and localized mainly at the sperm head and midpiece. U. parvum–infected mouse sperms exhibited decreased motility in a dose- and duration-dependent manner. Electron micrographs revealed that U. parvum infection induced the aggregation and morphological destruction of mouse sperm. Infected mouse sperm transported U. parvum into the fertilized egg with reduced fertilization rates, and infected embryo development was impaired. Conclusion(s): U. parvum infection caused deterioration of the mouse sperm quality and its functions, which affected the fertilization rate and embryo development.
• In vitro Antioxidant Activity of Mangifera indica Leaf Extracts

  Kimihisa Itoh; Tetsuya Matsukawa; Mamoru Okamoto; Kanasa Minami; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka

  Journal of Plant Studies Canadian Center of Science and Education 9 (2) 39 - 39 1927-0461 2020/06 [Refereed]
   

  In this study, we aimed to identify the utility of pruned mango (Mangifera indica ‘Irwin’) leaves as a resource for ingredients with antioxidant activity. Firstly, we examined the antioxidant activity of extracts obtained from the pericarps, flesh, flowers, barks, seeds, young dark reddish brown leaves (YDL-ext), young yellow leaves (YYL-ext), and pruned old dark green leaves (OML-ext) obtained from ‘Irwin’ mango. Among them, methanolic extract of flower and OML-ext showed the most potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity. The flesh extract showed weak DPPH radical scavenging activity, but did not show SOD-like activity. Secondly, we investigated the relationship between the maturation of leaves and their antioxidant activity by considering the contents of their two active polyphenolic components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1) and mangiferin (2), in addition to chlorophyll (3) and anthocyanins represented by cyanidin-3-O-glucoside (4). The DPPH radical scavenging activity of YDL-ext, YYL-ext and OML-ext were mainly attributable to 1, 2 and 3, whereas their SOD-like activity was partly attributable to 2. The DPPH radical scavenging and SOD-like activities of YDL-ext and YYL-ext were attributable to 1 and 2. These activities were also due to anthocyanins whose content is highest in YDL-ext. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for preparing cosmetics and/or supplemental ingredients with health-enhancing properties, antioxidant activity and inhibitory effect on AGEs formation and pancreatic lipase.
• Inhibitory Effect of Several Mangifera indica Cultivar Leaf Extracts on the Formation of Advanced Glycation End Products (AGEs)

  Kimihisa Itoh; Tetsuya Matsukawa; Kanasa Minami; Mamoru Okamoto; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka

  Journal of Plant Studies Canadian Center of Science and Education 9 (2) 33 - 33 1927-0461 2020/05 

  As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation.
• Pancreatic Lipase Inhibitory Activity of Citrus unshiu Leaf Extract

  Kimihisa Itoh; Tetsuya Matsukawa; Kazuya Murata; Ryota Nishitani; Momona Yamagami; Norimichi Tomohiro; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Shigeru Shigeoka; Yuichi Endo; Hideaki Matsuda

  Natural Product Communications SAGE Publications 14 (9) 1934578X1987343 - 1934578X1987343 1934-578X 2019/09 [Refereed]
   

  In the cultivation of Citrus fruit, pruning in late winter after harvest is important to obtain excellent flower buds in the next spring and a rich harvest of superior fruit in the following winter; a large number of pruned leaves are usually discarded. The purpose of this study was to determine the utility value of pruned leaves of Citrus unshiu. A methanol extract of pruned C. unshiu leaves showed a porcine pancreatic lipase inhibitory activity. Fractionation of C. unshiu leaf extract (CUL-ext) followed by bibliographic and chromatographic analyses revealed that a part of the pancreatic lipase inhibitory activity of CUL-ext was attributable to nobiletin (1), rutin (2), and hesperidin (3). Thus, pruned C. unshiu leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity.
• Promotion of lipopeptide antibiotic production by Bacillus sp. IA in the presence of rice husk biochar

  Pesticide Science

  Journal of pesticide science 44 (1) 33 - 40 1348-589X 2019/09 [Refereed]
   

  The purpose of this study is to isolate the beneficial microorganisms whose growth is promoted in the presence of charcoal materials. We successfully isolated strain IA, whose growth is promoted on an agar plate with charcoal materials, and identified it as a novel strain of the Bacillus sp. The growth of strain IA in the liquid medium was promoted by the addition of both activated charcoal (AC) and rice husk biochar (RHB). Moreover, the sporulation of strain IA in the RHB medium and the antifungal activity of the culture supernatant of the RHB medium were much higher than those with AC. HPLC and MS analyses revealed that strain IA produced an antifungal lipopeptide iturin A, and the yield of iturin A in the RHB medium was 8 times higher than that in the medium without RHB. This is the first paper to describe the positive effect of RHB on microbial metabolisms.
• Analysis of chemical diversity in Lilium japonicum population using floral scent composition

  Mizuki Fujisawa; Tetsuya Matsukawa; Toshiharu Akino; Shigeru Arai; Yoshihiro Takikawa; Shinichiro Kajiyama

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 258 0065-7727 2019/08
• Metabolic profiling of leaf secondary metabolites in Japanese citrus cultivars under stress conditions

  Tetsuya Matsukawa; Shinichiro Kajiyama

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 258 0065-7727 2019/08
• Metabolic profiling of secondary metabolites in phorbol ester containing and deficient Jatropha curcas seeds

  Kosuke Matsukubo; Tetsuya Matsukawa; Shinichiro Kajiyama

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 258 0065-7727 2019/08
• Purification and identification of a putative sperm chemoattractant in the liverwort Marchantia polymorpha L

  Yumiko Yamasaki; Miho Takemura; Tetsuya Matsukawa; Katsuyuki Yamato; Shinichiro Kajiyama

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 258 0065-7727 2019/08
• Antimicrobial activities by floral scents of the Japanese bamboo lily Lilium japonicum, the symbolic flowers offered the deity at the Saikusa Festival.

  Naoshi Hanasaki; Iku Iwasa; Yasuki, Takahashi; Aya Yanagawa; Shiichirou Kajiwara

  AROMA RESEARCH 78 (20) 41 - 47 1345-4722 2019/05 [Refereed][Invited]
  
• A Questionnaire-based Investigation Indicated Mental Relaxation Effects in the Scent of the Sacred Flower of the Saikusa Festival, Lilium japonicum

  藤澤 瑞希; 花崎 直史; 瀧川 義浩; 柳川 綾; 松川 哲也; 秋野 順治; 梶山 慎一郎; 荒井 滋

  生薬学雑誌 日本生薬学会 73 (1) 1 - 6 1349-9114 2019/02 [Refereed]
  
• 三枝祭の御神花ササユリLilium japonicum花香の静菌性

  秋野順治; 花崎直史; 岩佐郁; 藤澤瑞希; 高橋康樹; 梶山慎一郎; 松川哲也; 瀧川善浩; 柳川綾; 荒井滋

  人間・植物関係学会雑誌 19 (Supplement) 1346-7336 2019
• Past and Current trends in research of Lilium japnicum, sasayuri

  瀧川義浩; 田中普祟; 藤澤瑞希; 松川哲也; 梶山慎一郎

  近畿大学先端技術総合研究所紀要 (23) 11‐22  1346-8693 2018/03 [Refereed]
  
• Biological Control Potential of Streptomyces sp. AR10 Producing Albocycline Isolated from Soil around Ant Nest

  Tatsuya Ohike; Tetsuya Matsukawa; Masahiro Okanami; Shin’ichiro Kajiyama; Takashi Ano

  Journal of Agricultural Science 10 (3) 54 - 61 2018/01 [Refereed]
  
• In vitro and in vivo Assay for Assessment of the Biological Control Poterntial of Streptomyces sp. KT

  Tatsuya Ohike; Minori Maeda; Tetsuya Matsukawa; Masahiro Okanami; Shin’ichiro Kajiyama; Takashi Ano

  Journal of Plant Studies 7 (1) 10 - 18 2018/01 [Refereed]
  
• Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract

  Kimihisa Itoh; Kazuya Murata; Nao Sakaguchi; Kohei Akai; Tomoka Yamaji; Kohsuke Shimizu; Kaoru Isaki; Tetsuya Matsukawa; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki Matsuda

  Journal of Plant Studies Canadian Center of Science and Education 6 (2) 102 - 107 1927-0461 2017/07 [Refereed]
   

  The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.
• Protection and current situation of rare plant "Sasa yuri" in satoyama

  瀧川 義浩; 松川 哲也; 宮下 実; 梶山 慎一郎

  Memoirs of Institute of Advanced Technology, Kinki University 近畿大学先端技術総合研究所 (22) 35 - 40 1346-8693 2017/03 [Refereed]
   

  本論では里山におけるササユリの自生環境、個体数の減少の要因および近畿大学 先端技術総合研究所（研究所）と生物理工学部で実施している活動や研究について記載した。Sasa yuri（Lilium japnicum）is a wild lily in japan and also known as an index plant for evaluating a natural environment of satoyama. In the present paper, we described current situation and protection of the rare plant "Sasa yuri ".
• Metabolomic analysis of primary metabolites in citrus leaf during defense responses

  Tomonori Asai; Tetsuya Matsukawa; Shin'ichiro Kajiyama

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 123 (3) 376 - 381 1389-1723 2017/03 [Refereed]
   

  Mechanical damage is one of the unavoidable environmental stresses to plant growth and development. Plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. Jasmonic acid (JA) and salicylic acid (SA), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. However, defense related metabolites are so numerous and diverse that roles of individual compounds are still to be elucidated. In this report, we carried out a comprehensive analysis of metabolic changes during wound response in citrus plants which are one of the most commercially important fruit tree families. Changes in amino acid, sugar, and organic acid profiles in leaves were surveyed after wounding, JA and SA treatments using gas chromatography mass spectrometry (GC/MS) in seven citrus species, Citrus sinensis, Citrus limon, Citrus paradisi, Citrus unshiu, Citrus kinokuni, Citrus grandis, and Citrus hassaku. GC/MS data were applied to multivariate analyses including hierarchical cluster analysis (HCA), primary component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) to extract stress-related compounds. HCA showed the amino acid cluster including phenylalanine and tryptophan, suggesting that amino acids in this cluster are concertedly regulated during responses against treatments. OPLS-DA exhibited that tryptophan was accumulated after wounding and JA treatments in all species tested, while serine was down regulated. Our results suggest that tryptophan and serine are common biomarker candidates in citrus plants for wound stress. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
• Isolation and characterization of wound-induced compounds from the leaves of Citrus hassaku

  Tomonori Asai; Tetsuya Matsukawa; Atsushi Ishihara; Shin'ichiro Kajiyama

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 122 (2) 208 - 212 1389-1723 2016/08 [Refereed]
   

  Citrus plants are world widely cultivated as horticultural tree crops, and nowadays their pharmacological activities have been well studied. Since research of defense responses in citrus plants have been mainly focused on the post-harvested fruits because of their commercial importance, defense mechanisms during their developmental stages have not been well understood. In the present study, two wound-induced compounds were isolated from leaves of Citrus hassalcu, and their structures were elucidated by high-resolution electron spray ionization mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) analyses. One of these compounds was identified as a known flavanone, hesperetin. The other was characterized as a novel furofuran lignan, and was named `biscitrusnin-A'. Their antimicrobial activities were also evaluated. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
• A Pancreatic Lipase Inhibitory Activity by Mango (Mangifera indica) Leaf Methanolic Extract

  Kimihisa Itoh; Kazuya Murata; Yuta Nakagaki; Ayaka Shimizu; Yusuke Takata; Kohsuke Shimizu; Tetsuya Matsukawa; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki Matsuda

  Journal of Plant Studies 5 (2) 72 - 78 2016/06 [Refereed]
  
• Metabolic changes in Citrus leaf volatiles in response to environmental stress

  Tomonori Asai; Tetsuya Matsukawa; Shin'ichiro Kajiyamal

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 121 (2) 235 - 241 1389-1723 2016/02 [Refereed]
   

  Citrus plants are well known as a rich source of VOCs, and several have important roles in defense responses. However, how VOCs are regulated in response to environmental stress is not yet well understood. In this study, we investigated dynamic changes of VOCs present in leaves of seven Citrus species (Citrus sinensis, C. limon, C. paradisi, C. unshiu, C. kinokuni, C grandis, and C hassaku) in response to mechanical wounding, jasmonic acid (JA), and salicylic acid (SA) as determined by gas chromatography/mass spectrometric analysis followed by multivariate analysis (principal component analysis, PCA, and orthogonal partial least squares-discriminant analysis, OPLS-DA). PCA and OPLS-DA suggested that changes in VOC profiles against stress stimuli were much diverse among Citrus species. OPLS-DA showed that C6 volatiles, such as hexanal and trans-2-hexenal, were induced in response to JA and SA stimuli in C sinensis and C. grandis, while the other VOCs were decreased under all tested stress conditions. alpha-Farnesene was induced in all species except C. hassaku after wounding or JA treatment. In addition, alpha-farnesene was also induced in response to SA stimuli in C. unshiu and C kinokuni. Therefore these volatiles can be candidates of the common stress biomarkers in Citrus. Our results will give a new insight into defense mechanisms in Citrus species. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
• Lansioside D, a new triterpenoid glycoside antibiotic

  Eufrocinio C. Marfori; Shin Ichiro Kajiyama; Ei-Ichiro Fukusaki and

  Journal of Pharmacognosy and Phytochemistry 13 (5) 258 - 261 2015/05 [Refereed]
  
• バイオディーゼル原料植物Jatropha Curcasの毒性代謝産物とその制御

  MATSUKAWA TETSUYA; KAJIYAMA SHIN'ICHIRO

  スマートプロセス学会誌 Smart Processing Society for Materials, Environment & Energy (High Temperature Society of Japan) 3 (5) 258 - 261 2186-702X 2014/09 [Refereed]
  
• Potential application of oligotrophic bacteria as biocontrol agents

  Tatsuya Ohike; Tetsuya Matsukawa; Masahiro Okanami; Shin'ichiro Kajiyama; Takashi Ano

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 248 0065-7727 2014/08 [Refereed]
  
• マウス二段階発がん試験とEBV早期抗原誘導活性を指標としたJatropha curcas種子抽出物の発がんプロモーション活性評価

  MATSUKAWA TETSUYA; SHODA MASAHIRO; TAMAKI KEIKO; MURAKAMI HIKARU; KAJIYAMA SHIN'ICHIRO

  Mem Fac Biol Oriented Sci Technol Kinki Univ 近畿大学生物理工学部 33 (33) 9 - 16 1342-7202 2014/03 

  [要旨]トウダイグサ科の落葉低木であるJatropha curcasは, 種子に豊富な中性脂質を含み, 荒廃地でも生育可能であるため次世代バイオディーゼル原料植物として有望視されている。しかし一方で, 本植物の種子には発がんプロモーターであるphorbol ester類を含んでおり, 生産者や消費者の健康に与える負の影響が懸念されている。本研究ではJ. curcas 種子抽出物の発がんプロモーション活性を, マウスを用いた二段階発がん試験およびEBV早期抗原誘導活性を指標としたアッセイ系により評価した。その結果, J. curcas種子抽出物の発がんプロモーション活性は, 発がんプロモーション活性を有する代表的なホルポールエステルであるphorbol-12-myristate-13-acetate(PMA)より低いことが見いだされた。また，種子抽出物はPMAの発がん活性を抑制することが示された。これらの結果から, J. curcas種子抽出物には化学発がんを抑制する物質が含まれることが示唆された。 [Abstract] Jatr•opha eurcas, a tropical plant belonging to the family of Euphorbiaceac, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content in seed kernel. The seed, however, contains phorbol esters which exhibit tumor-promoting activity, and thus negative impact of these compounds on health of producers or consumers should be concerned_ In this study, tumor-promoting activity of I cur eas seed extracts was evaluated by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay. Our results showed that tumor-promoting activity of J. curcas seed extracts was significantly lower than phorbol-l2-myristate-1 3-acetate (PMA), which is well known as a potent tumor promotor. In addition, J. cur eas seed extracts were revealed to suppress the tumor-promoting activity ofPMA. These results suggest that./ curcas seed extracts contain some suppressor of tumorgenesis.
• 〈Original Papers〉 Evaluation of tumor promoting activity of Jatropha curcas seed extracts by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay.

  松川 哲也; 正田 将大; 玉置 恵子; 村上 ひかる; 梶山 慎一

  近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University 近畿大学生物理工学部 33 9 - 16 1342-7202 2014/03 

  [要旨]トウダイグサ科の落葉低木であるJatropha curcasは, 種子に豊富な中性脂質を含み, 荒廃地でも生育可能であるため次世代バイオディーゼル原料植物として有望視されている。しかし一方で, 本植物の種子には発がんプロモーターであるphorbol ester類を含んでおり, 生産者や消費者の健康に与える負の影響が懸念されている。本研究ではJ. curcas 種子抽出物の発がんプロモーション活性を, マウスを用いた二段階発がん試験およびEBV早期抗原誘導活性を指標としたアッセイ系により評価した。その結果, J. curcas種子抽出物の発がんプロモーション活性は, 発がんプロモーション活性を有する代表的なホルポールエステルであるphorbol-12-myristate-13-acetate(PMA)より低いことが見いだされた。また，種子抽出物はPMAの発がん活性を抑制することが示された。これらの結果から, J. curcas種子抽出物には化学発がんを抑制する物質が含まれることが示唆された。 [Abstract] Jatr•opha eurcas, a tropical plant belonging to the family of Euphorbiaceac, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content in seed kernel. The seed, however, contains phorbol esters which exhibit tumor-promoting activity, and thus negative impact of these compounds on health of producers or consumers should be concerned_ In this study, tumor-promoting activity of I cur eas seed extracts was evaluated by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay. Our results showed that tumor-promoting activity of J. curcas seed extracts was significantly lower than phorbol-l2-myristate-1 3-acetate (PMA), which is well known as a potent tumor promotor. In addition, J. cur eas seed extracts were revealed to suppress the tumor-promoting activity ofPMA. These results suggest that./ curcas seed extracts contain some suppressor of tumorgenesis.
• 天然物由来成分に騙されるな : 天然物は本当に安全なの?(続・生物工学基礎講座-バイオよもやま話-)

  松川 哲也; 梶山 慎一郎

  生物工学会誌 : seibutsu-kogaku kaishi 日本生物工学会 92 (10) 556 - 559 0919-3758 2014
• Plant metabolite analysis with laser optics.

  KAJIYAMA Shinichiro

  Regulation of Plant Growth & Development 植物化学調節学会 48 (2) 163 - 168 1346-5406 2013/12 [Invited]
   

  In recent decades, laser optics has been strenuously adapted to biological and biotechnological applications. Laser has high monochromaticity, directivity, and coherency and these properties are considered to be suitable for manipulating and/or observing intact cells with high special resolution. In this technical note, several laser-based microsurgery and non-linear optical microscopy techniques for plant metabolite analysis was discussed.
• Visualization of oil body distribution in Jatropha curcas L. by four-wave mixing microscopy

  Makiko Ishii; Susumu Uchiyama; Yasuyuki Ozeki; Sin'ichiro Kajiyama; Kazuyoshi Itoh; Kiichi Fukui

  Japanese Journal of Applied Physics 52 (6) 062403  0021-4922 2013/06 [Refereed]
   

  Jatropha curcas L. (jatropha) is a superior oil crop for biofuel production. To improve the oil yield of jatropha by breeding, the development of effective and reliable tools to evaluate the oil production efficiency is essential. The characteristics of the jatropha kernel, which contains a large amount of oil, are not fully understood yet. Here, we demonstrate the application of four-wave mixing (FWM) microscopy to visualize the distribution of oil bodies in a jatropha kernel without staining. FWM microscopy enables us to visualize the size and morphology of oil bodies and to determine the oil content in the kernel to be 33.2%. The signal obtained from FWM microscopy comprises both of stimulated parametric emission (SPE) and coherent anti-Stokes Raman scattering (CARS) signals. In the present situation, where a very short pump pulse is employed, the SPE signal is believed to dominate the FWM signal. © 2013 The Japan Society of Applied Physics.
• A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads

  Naoki Wada; Shin'ichiro Kajiyama; Naruemon Khemkladngoen; Kiichi Fukui

  Plant Transformation Technologies Wiley-Blackwell 73 - 81 2011/03 [Refereed]
  
• Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima.

  大池 達矢; 松川 哲也; 岡南 政宏; 阿野 貴司; 梶山 慎一郎

  Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University 近畿大学生物理工学部 (27) 29 - 37 1342-7202 2011/03 

  [要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60％の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60％) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
• Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima.

  大池 達矢; 松川 哲也; 岡南 政宏; 阿野 貴司; 梶山 慎一郎

  Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要 近畿大学生物理工学部 (27) 29 - 37 1342-7202 2011/03 

  [要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60％の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60％) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
• Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

  Shusei Sato; Hideki Hirakawa; Sachiko Isobe; Elgo Fukai; Akiko Watanabe; Midori Kato; Kumiko Kawashima; Chiharu Minami; Akiko Muraki; Naomi Nakazaki; Chika Takahashi; Shinobu Nakayama; Yoshie Kishida; Mitsuyo Kohara; Manabu Yamada; Hisano Tsuruoka; Shigemi Sasamoto; Satoshi Tabata; Tomoyuki Aizu; Atsushi Toyoda; Tadasu Shin-i; Yohei Minakuchi; Yuji Kohara; Asao Fujiyama; Suguru Tsuchimoto; Shin'ichiro Kajiyama; Eri Makigano; Nobuko Ohmido; Nakako Shibagaki; Joyce A. Cartagena; Naoki Wada; Tsutomu Kohinata; Alipour Atefeh; Shota Yuasa; Sachihiro Matsunaga; Kiichi Fukui

  DNA RESEARCH OXFORD UNIV PRESS 18 (1) 65 - 76 1340-2838 2011/02 

  The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for,similar to 95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 11529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.
• Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.

  Shusei Sato; Hideki Hirakawa; Sachiko Isobe; Elgo Fukai; Akiko Watanabe; Midori Kato; Kumiko Kawashima; Chiharu Minami; Akiko Muraki; Naomi Nakazaki; Chika Takahashi; Shinobu Nakayama; Yoshie Kishida; Mitsuyo Kohara; Manabu Yamada; Hisano Tsuruoka; Shigemi Sasamoto; Satoshi Tabata; Tomoyuki Aizu; Atsushi Toyoda; Tadasu Shin-i; Yohei Minakuchi; Yuji Kohara; Asao Fujiyama; Suguru Tsuchimoto; Shin'ichiro Kajiyama; Eri Makigano; Nobuko Ohmido; Nakako Shibagaki; Joyce A. Cartagena; Naoki Wada; Tsutomu Kohinata; Alipour Atefeh; Shota Yuasa; Sachihiro Matsunaga; Kiichi Fukui

  DNA RESEARCH OXFORD UNIV PRESS 18 (1) 65 - 76 1340-2838 2011/02 [Refereed]
   

  The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for,similar to 95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 11529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.
• Quantitative analysis of phorbol esters in the wastes and intermediate products of Jatropha bio-diesel production.

  松川 哲也; 梶山 慎一郎

  近畿大学生物理工学部紀要 近畿大学生物理工学部 (26) 13 - 22 1342-7202 2010/09 

  トウダイグサ科の熱帯性植物であるJatropha curcasは, 種子に重量比40-60％の中性脂質を含み, 半乾燥地や貧栄養土壌などの荒廃地でも生育可能であるため, 近年新たなバイオディーゼル燃料の原料植物として注目されている。しかし, 本植物の種子には発ガン促進物質であるphorbol esterを始めとする種々の毒性物質を含んでおり, その生産者の健康や環境への影響が懸念されている。そこで, 本研究では精油工程で産出される廃棄物や, 中間生成物に含まれるphorbol ester類の定量分析を行った。その結果, 搾油工程および脱ガム工程では中間生成物および廃棄物ともにphorbol ester類が多く含まれていた。一方, 脱酸工程, メチル化工程ではphorbol ester量が減少することが見いだされた。また, 最終生産物であるバイオディーゼル燃料からもphorbol ester類が微量検出された。本研究の結果は,J.curcas由来バイオディーゼルのリスク評価に寄与すると期待される。 (英文） Jatropha curcas, a tropical plant belonging to the family of Euphorbiaceae, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content (40-60％) in seed kernel. The seed, however, contains several toxic compounds including phorbol ester, well known as a tumor promoting agent. Therefore, influence of these toxic compounds on environment and people engaged in the production should be concerned. In this study, the levels of phorbol esters in the wastes and intermediate products of different refining steps were quantitatively analyzed. Our results showed that both intermediate products and wastes obtained from pressing and degumming processes exhibited high phorbol esters contents, whereas levels of phorbol esters decreased during neutralization and transesterification. In addition, bio-diesel fluid, the final products of refining steps, also contains trace amount of phorbol esters. Our results may contribute to assess the risk of J. curcas bio-diesel.本研究は近畿大学生物理工学部戦略的研究No. 08-II-02の助成を受けた. , 本研究の一部は住友財団奨励金の助成を受けた. , Kajiyama, Shin'ichiro
• 高調波同期した2波長光源による誘導ラマン散乱顕微鏡の高感度化

  小関泰之; 北川雄真; 梅村航; 住村和彦; 西澤典彦; 梶山慎一郎; 福井希一; 伊東一良

  2010/08 [Invited]
  
• Stimulated Raman scattering microscope with shot noise limited sensitivity using subharmonically synchronized laser pulses

  Yasuyuki Ozeki; Yuma Kitagawa; Kazuhiko Sumimura; Norihiko Nishizawa; Wataru Umemura; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  OPTICS EXPRESS OPTICAL SOC AMER 18 (13) 13708 - 13719 1094-4087 2010/06 [Refereed]
   

  We propose and demonstrate the use of subharmonically synchronized laser pulses for low-noise lock-in detection in stimulated Raman scattering (SRS) microscopy. In the experiment, Yb-fiber laser pulses at a repetition rate of 38 MHz are successfully synchronized to Ti:sapphire laser pulses at a repetition rate of 76 MHz with a jitter of <8 fs by a two-photon detector and an intra-cavity electro-optic modulator. By using these pulses, high-frequency lock-in detection of SRS signal is accomplished without high-speed optical modulation. The noise level of the lock-in signal is found to be higher than the shot noise limit only by 1.6 dB. We also demonstrate high-contrast, 3D imaging of unlabeled living cells. (C) 2010 Optical Society of America
• Structure and Functional Characterization of Vibrio parahaemolyticus Thermostable Direct Hemolysin

  Itaru Yanagihara; Kumiko Nakahira; Tsutomu Yamane; Shuji Kaieda; Kouta Mayanagi; Daizo Hamada; Takashi Fukui; Kiyouhisa Ohnishi; Shin'ichiro Kajiyama; Toshiyuki Shimizu; Mamoru Sato; Takahisa Ikegami; Mitsunori Ikeguchi; Takeshi Honda; Hiroshi Hashimoto

  JOURNAL OF BIOLOGICAL CHEMISTRY AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC 285 (21) 16267 - 16274 0021-9258 2010/05 [Refereed]
   

  Thermostable direct hemolysin (TDH) is a major virulence factor of Vibrio parahaemolyticus that causes pandemic foodborne enterocolitis mediated by seafood. TDH exists as a tetramer in solution, and it possesses extreme hemolytic activity. Here, we present the crystal structure of the TDH tetramer at 1.5 angstrom resolution. The TDH tetramer forms a central pore with dimensions of 23 angstrom in diameter and similar to 50 angstrom in depth. pi-Cation interactions between protomers comprising the tetramer were indispensable for hemolytic activity of TDH. The N-terminal region was intrinsically disordered outside of the pore. Molecular dynamic simulations suggested that water molecules permeate freely through the central and side channel pores. Electron micrographs showed that tetrameric TDH attached to liposomes, and some of the tetramer associated with liposome via one protomer. These findings imply a novel membrane attachment mechanism by a soluble tetrameric pore-forming toxin.
• The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant

  Naoki Wada; Shin'ichiro Kajiyama; Joyce A. Cartagena; Linyen Lin; Yukio Akiyama; Motoyasu Otani; Go Suzuki; Yasuhiko Mukai; Noriaki Aoki; Kiichi Fukui

  JOURNAL OF CEREAL SCIENCE ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 51 (2) 182 - 188 0733-5210 2010/03 

  Endosperm texture is an important factor governing the end-product quality of cereals. The texture of wheat (Triticum aestivum L) endosperm is controlled by puroindoline a and b genes which are both absent in rice (Oryza sativa L). It has been reported that the endosperm texture of rice can be modified by puroindoline genes. The mechanism, however, by which puroindolines affect the ultrastructure of rice endosperm cells remains to be investigated. In this study, we observed the ultrastructure of endosperm cells and the morphology of isolated starch granules of the transgenic rice expressing the puroindoline b gene. SEM and TEM observations indicated that compound starch granules were embedded within the matrix material in non-transgenic rice, Nipponbare, whereas they were surrounded by spaces in the transgenic rice. The morphology and size of each starch granule were not different between nontransgenic and the transgenic rice. However, the transgenic rice flour showed smaller particle size, higher starch damage, and lower viscosity during gelatinization than that of non-transgenic rice. These results confirm that puroindoline b reduces the grain hardness in rice. Moreover, the results also suggest that puroindoline b functions at the surface of compound starch granules, and not on polygonal starch granules in rice endosperm. (C) 2009 Elsevier Ltd. All rights reserved.
• Biological applications of novel nonlinear optical microscopy

  Shin'ichiro Kajiyama; Yasuyuki Ozeki; Kiichi Fukui; Kazuyoshi Itoh

  2009 INTERNATIONAL WORKSHOP ON INFORMATION OPTICS IOP PUBLISHING LTD 206 1742-6588 2010 

  Two types of newly developed nonlinear optical microscopes namely stimulated parametric emission (SPE) microscope and stimulated Raman scattering (SRS) microscope were presented together with their biological applications.
• Dual-Band Stimulated Parametric Emission Microscopy

  Masatomo Yamagiwa; Gen Omura; Yasuyuki Ozeki; Makiko Ishii; Hieu Min Dang; Shin'ichiro Kajiyama; Takeshi Suzuki; Kiichi Fukui; Kazuyoshi Itoh

  JAPANESE JOURNAL OF APPLIED PHYSICS JAPAN SOC APPLIED PHYSICS 49 (1) 16603 - 16606 0021-4922 2010 

  We demonstrate that two distinct images can be simultaneously acquired using the dual-band configuration of stimulated parametric emission (SPE) microscopy, which is based on the four-wave mixing process enhanced by two-photon electronic resonance. The dual-band SPE microscopy utilizes two SPE signals simultaneously generated at different wavelengths. In the experiment, we image stained and unstained biological tissues, and obtain two different images for each sample. Additionally, we propose an axial differential imaging technique to improve the contrast of SPE images, and verify its applicability to biological imaging. (C) 2010 The Japan Society of Applied Physics
• Enzymatic Production of (-)-Indolactam V by LtxB, a Cytochrome P450 Monooxygenase

  Minh U. Huynh; Matthew C. Elston; Nick M. Hernandez; David B. Ball; Shin-ichiro Kajiyama; Kazuhiro Irie; William H. Gerwick; Daniel J. Edwards

  JOURNAL OF NATURAL PRODUCTS AMER CHEMICAL SOC 73 (1) 71 - 74 0163-3864 2010/01 [Refereed]
   

  The P450 cytochrome monooxygenase gene, ltxB, was cloned and overexpressed in Escherichia coli as a 6xHis-taoged protein. The resulting recombinant LtxB was purified by Ni-NTA affinity chromatography and characterized biochemically. Purified LtxB demonstrated typical cytochrome P450 spectroscopic properties including substrate-induced transition from a low-spin (lambda(max) = 414 nm) to high-spin state (lambda(max) = 386 nm) upon incubation with N-methyl-L-valyl-L-tryptophanol. The catalytic activity of LtxB was verified by demonstrating the oxidation/cyclization of N-methyl-L-valyl-L-tryptophanol to (-)-indolactam V. LtxB shows a relaxed specificity for analogue substrates in which the valyl group is substituted for other aliphatic groups. The relaxed substrate specificity of LtxB, along with the relaxed specificity of the prenyltransferase, LtxC, allowed for the enzymatic production of a series of (-)-indolactam V and lyngbyatoxin analogues.
• Biological applications of stimulated parametric emission microscopy and stimulated raman scattering microscopy

  Shin'Ichiro Kajiyama; Yasuyuki Ozeki; Kiichi Fukui; Kazuyoshi Itoh

  Information Optics and Photonics: Algorithms, Systems, and Applications Springer New York 89 - 98 2010 [Refereed]
   

  Two types of newly developed nonlinear-optical microscopy are presented, namely, stimulated parametric emission (SPE) microscopy and stimulated Raman scattering (SRS) microscopy, together with their biological applications. These techniques allow high-resolution 3D visualization of unstained living cells. SPE microscopy is also applied to time-lapse observation of dividing cells as well as simultaneous observation of fluorescence emitted from labeled organelles and SPE signal. © 2010 Springer Science+Business Media, LLC.
• Subharmonic Synchronization of Two-Color Laser Pulses for Stimulated Raman Scattering Microscopy

  Yasuyuki Ozeki; Yuma Kitagawa; Kazuhiko Sumimura; Norihiko Nishizawa; Wataru Umemura; Makiko Ishii; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  2010 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO) AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (QELS) IEEE 2010 [Refereed]
   

  We successfully demonstrate low-jitter synchronization of a 38-MHz Yb-fiber oscillator to a 76-MHz Ti:sapphire oscillator with a two-photon detector and an intra-cavity electro-optic modulator for sensitive lock-in detection of stimulated Raman signal in biological microscopy. (C) 2010 Optical Society of America
• Three-Dimensional Unstained Live-Cell Imaging Using Stimulated Parametric Emission Microscopy

  Hieu M. Dang; Takehito Kawasumi; Gen Omura; Toshiyuki Umano; Shin'ichiro Kajiyama; Yasuyuki Ozeki; Kazuyoshi Itoh; Kiichi Fukui

  JAPANESE JOURNAL OF APPLIED PHYSICS JAPAN SOC APPLIED PHYSICS 48 (9) 97007  0021-4922 2009/09 

  The ability to perform high-resolution unstained live imaging is very important to in vivo study of cell structures and functions. Stimulated parametric emission (SPE) microscopy is a nonlinear-optical microscopy based on ultra-fast electronic nonlinear-optical responses. For the first time, we have successfully applied this technique to archive three-dimensional (3D) images of unstained sub-cellular structures, such as, microtubules, nuclei, nucleoli, etc. in live cells. Observation of a complete cell division confirms the ability of SPE microscopy for long time-scale imaging. (C) 2009 The Japan Society of Applied Physics
• Development of an enzyme activity screening system for beta-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting

  Yan Zhou; Shin'ichiro Kajiyama; Kouichi Itoh; Takanori Tanino; Nobuo Fukuda; Tsutomu Tanaka; Akihiko Kondo; Kiichi Fukui

  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER 84 (2) 375 - 382 0175-7598 2009/08 

  Recent reports on high-speed affinity screening systems for yeast cells using flow cytometry have not been adapted to screening yeast cells that display hydrolyzing enzymes, since the fluorescent molecules which are released from fluoresceinated substrate diffuse into solution after enzymatic reaction. In this research, yeast cells displaying beta-glycosidase were individually captured in micro-sized calcium alginate beads by using the newly developed reverse micelle method to prevent diffusion of hydrolyzed fluorescent substrates. By adopting flow sorting to these captured cells, active cells were successfully enriched about 82-fold from a mixed suspension with negative controls. This system should be a useful method for high-speed screening of yeast cells that display various hydrolyzing enzymes and has potential application to screening randomized libraries of enzyme-displayed yeast cells with higher activities.
• Development of an enzyme activity screening system for beta-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting

  Yan Zhou; Shin'ichiro Kajiyama; Kouichi Itoh; Takanori Tanino; Nobuo Fukuda; Tsutomu Tanaka; Akihiko Kondo; Kiichi Fukui

  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER 84 (2) 375 - 382 0175-7598 2009/08 [Refereed]
   

  Recent reports on high-speed affinity screening systems for yeast cells using flow cytometry have not been adapted to screening yeast cells that display hydrolyzing enzymes, since the fluorescent molecules which are released from fluoresceinated substrate diffuse into solution after enzymatic reaction. In this research, yeast cells displaying beta-glycosidase were individually captured in micro-sized calcium alginate beads by using the newly developed reverse micelle method to prevent diffusion of hydrolyzed fluorescent substrates. By adopting flow sorting to these captured cells, active cells were successfully enriched about 82-fold from a mixed suspension with negative controls. This system should be a useful method for high-speed screening of yeast cells that display various hydrolyzing enzymes and has potential application to screening randomized libraries of enzyme-displayed yeast cells with higher activities.
• Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes

  Hieu M. Dang; Gen Omura; Toshiyuki Umano; Masatomo Yamagiwa; Shin'ichiro Kajiyama; Yasuyuki Ozeki; Kazuyoshi Itoh; Kiichi Fukui

  JOURNAL OF BIOMEDICAL OPTICS SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS 14 (4) 040506  1083-3668 2009/07 [Refereed]
   

  We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3207151]
• Physical Mapping of Ribosomal DNA Genes on Jatropha curcas Chromosomes by Multicolor FISH

  Magdalena Witkowska; Nobuko Ohmido; Joyce Cartagena; Nakako Shibagaki; Shin'ichiro Kajiyama; Kiichi Fukui

  CYTOLOGIA UNIV TOKYO CYTOLOGIA 74 (2) 133 - 139 0011-4545 2009/06 

  Jatropha curcas L. belonging to the Euphorbiaceae is becoming a significant crop in tropical areas nowadays. The genetic diversity among 1 African and 5 Asian varieties were analyzed by multicolor fluorescence in situ hybridization (McFISH) using 5S and 45S ribosomal RNA genes (rDNAs). One locus of 5S rDNA and 2 loci for 45S rDNA were detected at specific regions of the chromosomes in all the materials. Also, telomeric repeats (TTTAGGG)(n) were localized on the terminal regions of all chromosomes. The present results confirm the stability of these major repeated sequences among Jatropha lines.
• Nonlinear Phase Imaging Using Two-Beam Interferometry in Stimulated Parametric Emission Microscopy

  Masatomo Yamagiwa; Yasuyuki Ozeki; Gen Omura; Takeshi Suzuki; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  JAPANESE JOURNAL OF APPLIED PHYSICS JAPAN SOCIETY APPLIED PHYSICS 48 (6) 062501  0021-4922 2009/06 

  We demonstrate that two-beam interferometry is applicable to phase detection of nonlinear susceptibility of biological samples in the stimulated parametric emission (SPE) microscopy, which is one of the coherent nonlinear optical microscopy techniques. We experimentally observed stained mouse kidney cells and successfully confirmed nonlinear phase shift associated with an electronic resonance at stained nuclei. In addition, phase blurring was found to be almost completely eliminated in a nonlinear differential interferometric contrast image, which can be produced through a simple post-processing procedure. (C) 2009 The Japan Society of Applied Physics
• Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes

  Naoki Wada; Shin'ichiro Kajiyama; Yukio Akiyama; Shigeki Kawakami; Daisuke No; Susumu Uchiyama; Motoyasu Otani; Takiko Shimada; Naoko Nose; Go Suzuki; Yasuhiko Mukai; Kiichi Fukui

  PLANT CELL REPORTS SPRINGER 28 (5) 759 - 768 0721-7714 2009/05 [Refereed]
   

  Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T(2) generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously.
• High-resolution spatial and temporal analysis of phytoalexin production in oats

  Yoshihiro Izumi; Shin'ichiro Kajiyama; Ryosuke Nakamura; Atsushi Ishihara; Atsushi Okazawa; Eiichiro Fukusaki; Yasuo Kanematsu; Akio Kobayashi

  PLANTA SPRINGER 229 (4) 931 - 943 0032-0935 2009/03 [Refereed]
   

  The production of oat (Avena sativa L.) phytoalexins, avenanthramides, occurs in response to elicitor treatment with oligo-N-acetylchitooligosaccharides. In this study, avenanthramides production was investigated by techniques that provide high spatial and temporal resolution in order to clarify the process of phytoalexin production at the cellular level. The amount of avenanthramides accumulation in a single mesophyll cell was quantified by a combination of laser micro-sampling and low-diffuse nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) techniques. Avenanthramides, NAD(P)H and chlorophyll were also visualized in elicitor-treated mesophyll cells using line-scanning fluorescence microscopy. We found that elicitor-treated mesophyll cells could be categorized into three characteristic cell phases, which occurred serially over time. Phase 0 indicated the normal cell state before metabolic or morphological change in response to elicitor, in which the cells contained abundant NAD(P)H. In phase 1, rapid NAD(P)H oxidation and marked movement of chloroplasts occurred, and this phase was the early stage of avenanthramides biosynthesis. In phase 2, avenanthramides accumulation was maximized, and chloroplasts were degraded. Avenanthramides appear to be synthesized in the chloroplast, because a fluorescence signal originating from avenanthramides was localized to the chloroplasts. Moreover, our results indicated that avenanthramides biosynthesis and the hypersensitive response (HR) occurred in identical cells. Thus, the avenanthramides production may be one of sequential events programmed in HR leading to cell death. Furthermore, the phase of the defense response was different among mesophyll cells simultaneously treated with elicitor. These results suggest that individual cells may have different susceptibility to the elicitor.
• Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy

  Yasuyuki Ozeki; Fumihiro Dake; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  OPTICS EXPRESS OPTICAL SOC AMER 17 (5) 3651 - 3658 1094-4087 2009/03 [Refereed]
   

  We theoretically show that the shot-noise-limited sensitivity of stimulated Raman scattering (SRS) microscopy, which enables high-contrast vibrational imaging, is similar to that of coherent anti-Stokes Raman scattering microscopy. We experimentally confirm that the sensitivity of our SRS microscope is lower than the shot-noise limit only by <15 dB, which indicates that the high-sensitivity of SRS microscopy is readily available. (C) 2009 Optical Society of America
• A new size and shape controlling method for producing calcium alginate beads with immobilized proteins

  Y. Zhou; 梶山 慎一郎; Hiroshi Masuhara; Yoichiro Hosokawa; Takahiro Kaji; Kiichi Fukui

  J.Biomed. Sci Eng. 2 (5) 287 - 293 2009
• Background-Free, 3D Vibrational Imaging by Stimulated Raman Scattering Microscopy

  Yasuyuki Ozeki; Fumihiro Dake; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5 IEEE 143 - + 2009 [Refereed]
   

  We propose and experimentally show that stimulated Raman scattering can realize novel 3D nonlinear-optical Raman microscopy that is immune to nonresonant background signals. We demonstrate high-contrast molecular imaging of polystyrene beads and plant cells. (C) 2009 Optical Society of America
• Highly Sensitive Signal Detection in Stimulated Parametric Emission Microscopy Based on Two-Beam Interferometry

  Masatomo Yamagiwa; Yasuyuki Ozeki; Takehito Kawasumi; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  JAPANESE JOURNAL OF APPLIED PHYSICS JAPAN SOCIETY APPLIED PHYSICS 47 (12) 8820 - 8824 0021-4922 2008/12 

  We present a simple and highly sensitive optical detection method based on two-beam interferometry for application to coherent nonlinear optical microscopy (CNOM). The theoretical sensitivity of this method is higher than that of conventional spectral interferometry (SI). We experimentally applied this technique to stimulated parametric emission (SPE) microscopy and achieved a high sensitivity that is only 4 or 5 dB lower than that of a theoretical shot noise limit. In order to validate the practical applicability of this technique, we demonstrated a noise reduction experiment in the observation of a plant cell with an SPE microscope. [DOI: 10.1143/JJAP.47.8820]
• Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation

  Shin'ichiro Kajiyama; Benesh Joseph; Fumihide Inoue; Masaki Shimamura; Eiichiro Fukusaki; Kenichi Tomizawa; Akio Kobayashi

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 106 (2) 194 - 198 1389-1723 2008/08 

  In this paper, we report a novel method for delivering genes into chloroplasts of tobacco cells using laser microablation. The plasmid pLD200-GFP was introduced into chloroplasts of Nicotiana tabacum cv. Xanthi guard cells and transient GFP expression was detected in the chloroplasts after 2-3 d of incubation. The technique uses an argon fluoride (ArF) excimer laser to perforate the cell surface in a 4 mu m(2) area in the presence of plasmid coated gold microparticles. Pretreatment of guard cells to promote stomatal closure prior to laser ablation resulted in a significant increase in the survival rate of cells and a transient expression rate of 2-3% in trial number basis was archived. Our method has unique advantages such as avoiding laborious pretreatments that adversely affect cell viability and specific delivery of transgenes into a desired cell in complex leaf tissue. This technique is a potential tool for cell specific transient gene expression studies for elucidation of gene regulation and expression.
• Supergiant ampholytic sugar chains with imbalanced charge ratio form saline ultra-absorbent hydrogels

  Maiko K. Okajima; Takeshi Bamba; Yasuhiro Kaneso; Kazumasa Hirata; Eiichiro Fukusaki; Shin'ichiro Kajiyama; Tatsuo Kaneko

  MACROMOLECULES AMER CHEMICAL SOC 41 (12) 4061 - 4064 0024-9297 2008/06
• Line-Scanning Microscopy for Time-Gated and Spectrally Resolved Fluorescence Imaging

  Ryosuke Nakamura; Yoshihiro Izumi; Shin'ichiro Kajiyama; Akio Kobayashi; Yasuo Kanematsu

  JOURNAL OF BIOLOGICAL PHYSICS SPRINGER 34 (1-2) 51 - 62 0092-0606 2008/04 

  Laser-scanning fluorescence microscopy for efficient acquisition of time-gated and spectrally resolved fluorescence images was developed based on line illumination of the laser beam and detection of the fluorescence image through a slit. In this optical arrangement, the fluorescence image was obtained by scanning only one axis perpendicular to the excitation line, and the acquisition time was significantly reduced compared with conventional laser-scanning confocal microscopy. A multidimensional fluorescence dataset consisting of fluorescence intensities as a function of x-position, y-position, fluorescence wavelength, and delay time after photoexcitation was analyzed and decomposed based on the parallel factor analysis model. The performance of the line-scanning microscopy was examined by applying it to the analysis of one of the plant defense responses, accumulation of antimicrobial compounds of phytoalexin in oat (Avena sativa), induced by the elicitor treatment.
• Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material

  Tomoko Murakawa; Shin'ichiro Kajiyama; Tomohiko Ikeuchi; Shigeki Kawakami; Kiichi Fukui

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 105 (1) 77 - 80 1389-1723 2008/01 

  The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method.
• Protection of isolated chromosomes using calcium-alginate microbeads (bioactive beads) toward chemical and physical stress.

  Murakawa, T; Hayashihara, K; Ito, K; Doi, T; Kajiyama, S; Fukui, K

  Chromosome Science 10 83 - 87 2008
• Improvement of bioactive bead-mediated transformation by concomitant application of electroporation

  Tomoko Murakawa; Shin'ichiro Kajiyama; Kiichi Fukui

  PLANT BIOTECHNOLOGY JAPANESE SOC PLANT CELL & MOLECULAR BIOL 25 (4) 387 - 390 1342-4580 2008 

  Techniques of exogenous gene introduction in plant cells have been actively applied for producing transgenic cultured cells that produce high levels of useful biological substances and are used for the molecular breeding of plants. A novel gene transfer technique has been developed using calcium alginate micro-beads (bioactive beads) to entrap genetic material. Although this technique has several advantages such as high transformation efficiency and the ability to introduce large DNA molecules, the precise optimal application conditions remain to be determined. Here, the optimal conditions for the concomitant use of bioactive beads and electroporation, such as polyethylene glycol (PEG) concentration, alginate concentration, electric field intensity, pulse duration, and pulse numbers, were determined for improved gene transfer efficiency of bioactive bead-mediated plant transformation. Consequently, highly efficient transient transgene expression (up to 4.7%) was achieved by applying electrical pulses (0.75 kV cm(-1), 30 mu s, 3 x) to a protoplast suspension in 12% polyethylene glycol (PEG).
• Femtosecond pulsed laser as a microscalpel for microdissection and isolation of specific sections from biological samples

  Masaaki Sakakura; Shinichiro Kajiyama; Masafumi Tsutsumi; Jinhai Si; Eiichiro Fukusaki; Yutaka Tamaru; Shin-Ichi Akiyama; Kiyotaka Miura; Kazuyuki Hirao; Mitsuyoshi Ueda

  JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS INST PURE APPLIED PHYSICS 46 (9A) 5859 - 5864 0021-4922 2007/09 

  The fermosecond laser is a candidate tool for dissecting and obtaining specific tissues or cells from biological samples. In this paper, we report on the dissection of flammable (heat-sensitive) plant tissues (Eucommia ulmoides stem) and that of a transparent zebrafish embryo placed in water. During laser dissection in the plant tissues, we observed that a low repetition rate of the irradiations is crucial for reducing the risk of thermal damage. During laser dissection of the zebrafish embryo, the laser-induced bubble formation at the tissue surface disturbed the laser-induced dissection. We concluded that the "interior laser dissection method", in which the dissection is performed by moving the laser spot inside a biological sample, is an efficient way to dissect a sample that is placed in water. In view of our findings, the advantages of fs laser dissection are discussed on the basis of the physical characteristics of fs laser machining.
• Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser

  Shin'ichiro Kajiyama; Fumihide Inoue; Yoshinobu Yoshikawa; Takeshi Shoji; Eiichiro Fukusaki; Akio Kobayashi

  PLANT BIOTECHNOLOGY JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY 24 (3) 315 - 320 1342-4580 2007/06 

  Novel methods for foreign-gene introduction into specific cells are required for the functional analysis of specific cells. We report a new transfection method for intact plant cells that uses ArF excimer laser-induced shock waves to introduce foreign gene-coated gold particles into plant cells through the cell wall. Foreign gene (sGFP)-coated gold particles were randomly scattered on the surface of Torenia tissue samples; the laser was irradiated toward the gold particles using a hollow optical fiber and a quartz light-condensing tip to introduce the particles into the target cells. Treated plant tissue samples were then cultured for several weeks on the selection medium, and transformation was assessed by fluorescence microscopy and PCR. Irradiation-induced damage to the cells and coated DNA was insignificant, and the transformation efficiency was similar to that of biolistic transformation based on the particle number. Although this method requires a suitable laser setup and is time-consuming, it could be applied in transformation experiments on specific target cells. Moreover, this method can possibly be used to introduce not only DNA but also biologically active substances such as protein regulators or inhibitors, whose introduction into cells is difficult through the cell wall.
• 大阪大学大学教育実践センター 平成18年度1学期 体験的課題追求型授業「植物を知り、植物に学ぶ」、 体験的課題追求型授業プロジェクト報告書

  栗原佐智子; 梶山慎一郎; 齊藤修; 松永幸大; 近江戸伸子; 米田該典; 福井希一

  2007/03
• レーザーを用いた細胞のサンプリングと分析

  梶山慎一郎

  レーザ加工学会誌 14 (1) 1881-6797 2007
• Single cell sampling and analysis by ultra short pulse laser

  Kajiyama Shinichiro; Sakakura Masaaki; Ueda Mitsuyoshi; Itoh Kazuyoshi; Fukui Kiichi

  PROGRESS ON POST-GENOME TECHNOLOGIES PHOENIX PUBL & MEDIA NETWORK 25 - 28 2007 [Refereed]
   

  In order to obtain molecular information on single - cell basis, two new techniques which utilized ultra short pulse laser were developed. The first technique was the laser micro - scalpel for biological samples, which enables precise dissection of beat labile and/or water embedded samples. Using this technique, the highly farmable tissue of Eucommia plant and water embedded zebrafish embryo were successfully dissected. The second technique was novel non - liner four wave mixing microscope named stimulated parametric emission ( SPE) microscopy. This technique was applied to observe cell components of the non - stained living tobacco BY - 2 cells.
• Single cell-based analysis of Torenia petal pigments by a combination of ArF excimer laser micro sampling and nano-high performance liquid chromatography (HPLC)-mass spectrometry

  Shin'ichiro Kajiyama; Kazuo Harada; Eiichiro Fukusaki; Akio Kobayashi

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 102 (6) 575 - 578 1389-1723 2006/12 

  The molecular constituents of the petal pigments of the Torenia plant (Torenia hybrida) were analyzed on a single-cell basis by a combination of newly developed laser-microsampling and nano-flow liquid chromatography-electro spray ionization mass spectrometry (LC-ESIMS) techniques. Our method should provide a facile method for obtaining precise metabolic profiles of each cell in a single plant tissue.
• JST Innovation Plaza KYOTO UEDA Project(Gravure & Interview,Precision Engineering for Visualization of Biological Functions)

  YABUKI Akihiko

  Journal of the Japan Society of Precision Engineering 公益社団法人精密工学会 72 (11) 1307 - 1310 0912-0289 2006/11
• JST Innovation Plaza KYOTO UEDA Project

  UEDA Mitsuyoshi; KAJIYAMA Shin'ichiro; YABUKI Akihiko

  精密工学会誌 = Journal of the Japan Society of Precision Engineering 72 (11) 1307 - 1310 0912-0289 2006/11
• A novel microsurgery method for intact plant tissue at the single cell level using ArF excimer laser microprojection

  S Kajiyama; T Shoji; S Okuda; Y Izumi; E Fukusaki; A Kobayashi

  BIOTECHNOLOGY AND BIOENGINEERING JOHN WILEY & SONS INC 93 (2) 325 - 331 0006-3592 2006/02 

  A novel microsurgery technique for the partial removal of rigid cell-walls in intact plant tissue is established. Using a size-variable slit, an ArF excimer laser was microprojected on the surface of the targeted cell, and this method enabled the area- and depth-controllable processing of the cortical structure of plant cells including the cuticle and cell wall layer. In epidermal cells of all tested plants, viabilities of more than 90% were retained 24 h after irradiation. Scanning electron microscope (SEM) observation revealed that the cuticle layer of the irradiated region was completely ablated, and the cellulose microfibrils of the secondary cell wall were partially removed; furthermore, 4 days after laser treatment, the regeneration of cell wall fibrils was observed. As a model experiment, the transient expression of synthetic green fluorescent protein (sGFP) was performed by the microinjection of cauliflower mosaic virus (CMV) 35S promoter-derived sGFP gene through an "aperture" in the treated cell surface. Moreover, micron-sized fluorescent beads were successfully introduced by the same method into the onion cells indicating that this method can be used to introduce foreign materials as large as organelles. (c) 2005 Wiley Periodicals, Inc.
• Highly sensitive spectral interferometric fourwave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy

  Keisuke Isobe; Yasuyuki Ozeki; Takehito Kawasumi; Shogo Kataoka; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  Optics Express Optical Society of American (OSA) 14 (23) 11204 - 11214 1094-4087 2006 

  We present spectral interferometric four-wave mixing (FWM) microscopy with a nearly shot-noise limited sensitivity and with the capability of separating FWM signals from fluorescence signals. We analyze the requirements for obtaining the shot-noise limited sensitivity and experimentally achieve the sensitivity that is only 4-dB lower than the shotnoise limit. Moreover, we show that only FWM signals can be extracted through the Fourier filtering even when the FWM spectrum is overlapped and overwhelmed by the fluorescence spectrum. We demonstrate simultaneous acquisition of FWM and two-photon excited fluorescence images of fluorescent monodispersed polystyrene microspheres. © 2006 Optical Society of America.
• Syntheses and screening tests of new chlorin derivatives as photosensitizer

  Y Nakae; E Fukusaki; S Kajiyama; A Kobayashi; S Nakajima; Sakata, I

  JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY ELSEVIER SCIENCE SA 174 (3) 187 - 193 1010-6030 2005/09 

  New chlorin derivatives were synthesized from protoporphyrin IX dimethyl ester (1) as a starting material. The tumor-localizing abilities were determined by bathochromic and hyperchromic shift test using albumin and the photosensitivities were evaluated by photooxidation test using dansyl-L-methionine. NOH-P-H (3) was the most excellent in the both tests. NOH-P-H (3) was conjugated with aspartic acid to evaluate the biodistribution with the time progress by nitrogen pulsed laser spectrofluorometry. The concentration of NOH-P-Asp (15) in cancer tissues showed the highest concentration at 6 h after the administration and corresponded to five to seven times in other organs. The concentration in cancer tissues showed a rapid decrease at 24 h after the administration. PDT effect of NOH-P-Asp (15) was evaluated using tumor-bearing mice. Remarkable anti-tumor effect was observed. NOH-P-Asp (15) was expected to be applicable to a new photosensitizer for PDT. (c) 2005 Elsevier B.V. All rights reserved.
• The convenient screening method using albumin for the tumor localizing property of Ga-porphyrin complexes

  Y Nakae; EI Fukusaki; S Kajiyama; A Kobayashi; S Nakajima; Sakata, I

  JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY ELSEVIER SCIENCE SA 172 (1) 55 - 61 1010-6030 2005/05 

  Eleven Ga-phorphyrin complexes bearing various lengths of carbon-chain were synthesized and the UV-vis spectra shift patterns in various concentrations of albumin solutions were evaluated. The distributions of these derivatives into the organs were also determined by surface fluorescence using nitrogen-pulsed laser spectrophotometry. The shift patterns between 0 and 0.9% albumin concentrations (albumin test) were correlated to their tumor localizing property. The albumin test was proved to be useful for evaluation of tumor localizing properties of porphyrin derivatives. The tumor localizing ability was increased along with increasing their lipophilicity and the derivative bearing decyl groups as side chains was a maximum. The porphyrin-albumin complex might have key mission for the passive accumulation focusing on the tumor. © 2004 Elsevier B.V. All rights reserved.
• Convenient screening methods for the photosensitivity

  Y Nakae; E Fukusaki; S Kajiyama; A Kobayashi; Sakata, I

  JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY ELSEVIER SCIENCE SA 171 (1) 91 - 95 1010-6030 2005/04 

  Two kinds of convenient photosensitivity screening methods (Methods A and B) were established to find new photosensitizers for photodynamic therapy (PDT). Method A is based on oxidation mechanism of cholesterol and types 1 and 11 can be specified. Method B is performed using dansyl-L-methionine and offers the benefits of fast response time. Various porphyrin, chlorin and phthalocyanine derivatives were synthesized and subjected to the screening methods. The assays have yielded similar results. The results were also identical to the photosensitivity (Ga > Zn > Mn-complex) of water-soluble metalloporphyrins bearing the same side chains. Therefore, the Methods A and B were thought to be appropriate as screening methods to find lead compounds of photosensitizers for PDT. (c) 2004 Elsevier B.V. All rights reserved.
• レーザー1細胞サンプリングを用いた植物代謝産物分析

  梶山 慎一郎

  Journal of pesticide science 30 (1) 57 - 60 1348-589X 2005/02
• Isolation and characterization of a cDNA encoding phytochrome a in the non-photosynthetic parasitic plant, Orobanche minor sm.

  C Trakulnaleamsai; A Okazawa; CI An; S Kajiyama; E Fukusaki; K Yoneyama; Y Takeuchi; A Kobayashi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 69 (1) 71 - 78 0916-8451 2005/01 

  In this study, the isolation and characterization of a phytochrome A (PHYA) homologous cDNA (OmPHYA) in the non-photosynthetic holoparasitic plant Orobanche minor are described. The present findings provide the first report of the presence of a PHYA homolog in the holoparasite. This study found that OmPHYA is of similar size to the other PHYAs of green plants and shows 72, 77, and 77% amino acid sequence identity with PHYA in Arabidopsis, potato, and tobacco respectively. The OmPHYA contains a conserved chromophore attachment cysteine at position 323. Although OmPHYA shows high sequence identity with other PHYAs in green plants, 13 amino acid substitutions located in both the N and C-terminal domains are observed (a total of 26 amino acids). OmPHYA is encoded by a single gene within the O. minor genome. The abundance of the OmPHYA transcript as well as nuclear translocation of OmphyA occurs in a light-dependent manner.
• Plant metabolite analysis using laser single cell sampling technique

  S Kajiyama

  JOURNAL OF PESTICIDE SCIENCE PESTICIDE SCI SOC JAPAN 30 (1) 57 - 60 1348-589X 2005
• 植物細胞レーザー加工技術の開発とその応用 (特集 バイオテクノロジーイノベーション(part1)単細胞解析をめざしたバイオと異分野の融合がもたらすブレークスルー技術の新潮流)

  小林 昭雄; 梶山 慎一郎

  バイオインダストリー シーエムシー出版 21 (11) 7 - 13 0910-6545 2004/11
• The LASER Micro-Processing of Living Cells and Its Application for Plant Biotechnology

  KOBAYASHI Akio; KAJIYAMA Shin'ichiro

  化学と生物 日本農芸化学会 42 (9) 596 - 603 0453-073X 2004/09
• Biosynthetic pathway for the C45 polyprenol, solanesol, in tobacco

  E Fukusaki; S Takeno; T Bamba; H Okumoto; H Katto; S Kajiyama; A Kobayashi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 68 (9) 1988 - 1990 0916-8451 2004/09 

  Feeding experiments were independently performed with [1-C-13]deoxy-D-xylulose triacetate and (RS)-[2-C-13]mevalonolactone in the tobacco plant. The labeling pattern for solanesol was elucidated to reveal that the isoprene moiety of solanesol would be derived from deoxy-xylulose. The result strongly suggests that tobacco solanesol is biosynthesized via the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway.
• Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference

  E Fukusaki; K Kawasaki; S Kajiyama; CI An; K Suzuki; Y Tanaka; A Kobayashi

  JOURNAL OF BIOTECHNOLOGY ELSEVIER SCIENCE BV 111 (3) 229 - 240 0168-1656 2004/08 

  Suppression of biosynthetic genes involved in flower color formation is an important approach for obtaining target flower colors. Here we report that flower color of the garden plant Torenia hybrida was successfully modulated by RNA interference (RNAi) against a gene of chalcone synthase (CHS), a key enzyme for anthocyanin and flavonoid biosynthesis. By using each of the coding region and the 3'-untranslated region of the CHS mRNA as an RNAi target, exhaustive and gene-specific gene silencing were successfully induced, and the original blue flower color was modulated to white and pale colors, respectively. Our results indicate that RNAi is quite useful for modulations of flower colors of commercially important garden plants. (C) 2004 Elsevier B.V. All rights reserved.
• A chitin-oligomer binding peptide obtained by screening of a phage display random peptide library and its affinity modulation corresponding to oxidation-reduction state

  E Fukusaki; K Ogawa; A Okazawa; SI Kajiyama; A Kobayashi

  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC ELSEVIER SCIENCE BV 28 (4-6) 181 - 184 1381-1177 2004/06 

  Screening of a phage display random 9-mer peptide library, in which cysteine residues were at the both terminals of the 7-mer random region, was performed to obtain an oligopeptide that recognizes a chitin-oligomer. Affinity of the obtained peptide (Cys-Ser-Arg-Thr-Thr-Arg-Thr-Arg-Cys) to chitotriose was modulated by its oxidation-reduction state. Only the oxidized form exhibited specific binding to the target molecule, chitotriose. This is the first report of reversible affinity modulation of a synthetic oligopeptide which can recognize a neutral saccharide. (C) 2004 Elsevier B.V. All rights reserved.
• A Novel Gene Delivery System in Plants with Calcium Alginate

  曽根 岳史; 長森 英二; 池内 智彦; 水上 温司; 高倉 友紀子; 梶山 慎一郎; 福崎 英一郎; 原島 俊; 小林 昭雄; 福井 希一

  Seibutsu-kogaku Kaishi 公益社団法人日本生物工学会 82 (3) 102 - 102 0919-3758 2004/03
• The LASER Micro-Processing of Living Cells and Its Application for Plant Biotechnology

  KOBAYASHI Akio; KAJIYAMA Shin'ichiro

  KAGAKU TO SEIBUTSU Japan Society for Bioscience, Biotechnology, and Agrochemistry 42 (9) 596 - 603 0453-073X 2004
• Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA

  A Mizukami; E Nagamori; Y Takakura; S Matsunaga; Y Kaneko; S Kajiyama; S Harashima; A Kobayashi; K Fukui

  BIOTECHNIQUES BIOTECHNIQUES OFFICE 35 (4) 734 - + 0736-6205 2003/10 

  Yeast artificial chromosomes (YACs) are useful cloning vectors that have the capacity to carry large DNA inserts. The largest barrier to using such large DNA molecules in transformation experiments has been their physical instability in solution. We developed a new method of transforming yeast using chromosome-sized DNA. The method uses calcium alginate microbeads to immobilize high-density yeast chromosomal DNA. Chromosomal DNA immobilized on microbeads is physically stabilized compared with naked chromosomal DNA. The microbead-mediated transformation performed well, not only with respect to the transformation frequency with large DNA molecules (> 100 kb) but also in successful tran formation using split chromosome DNA that exceeded 450 kb.
• Chemical and Biochemical Aspects of Desiccation Tolerant Cyanobacterium Nostoc commune (The Seminar, "Stresses and Responses and Adaptation of Living Organisms : Molecular and Cellular Mechanisms and its Application")

  KAJIYAMA Shinichiro; KOBAYASHI Akio

  Cryobiology and cryotechnology 低温生物工学会 49 (1) 37 - 42 1340-7902 2003/08 

  Nostoc commune, a nitrogen-fixing filamentous cyanobacterium, produces copious amounts of the EPS (extracellular polysaccharide) around the cells and has remarkable desiccation tolerance. Recently, biochemical experiment revealed that the EPS can serve to stabilize cyanobacterial cells during desiccation and subsequent rehydration. The EPS also seems to act as the physical and/or chemical barrier to the surroundings. Despite marked features of EPS of this cyanobacteria, little is known about its chemical structure, due largely to structural complexity. In this paper, the chemical and biochemical aspects of this cyanobacterial EPS are discussed.
• Phytotoxicity of the tetramic acid metabolite trichosetin

  EC Marfori; SI Kajiyama; EI Fukusaki; A Kobayashi

  PHYTOCHEMISTRY PERGAMON-ELSEVIER SCIENCE LTD 62 (5) 715 - 721 0031-9422 2003/03 

  Trichosetin, a tetramic acid-containing metabolite produced in the dual culture of Trichoderma harzianum and Catharanthus roseus (L.) G. Don callus, was subjected to phytotoxicity assays. In seedling growth assays, trichosetin inhibited root and shoot growth of all five plant species tested by damaging the cell membrane, as evidenced by the dose-dependent increase in electrolyte leakage and lipid peroxidation. Vital staining of trichosetin-treated Nicotiana tabacum BY-2 cells, with rhodamine 123, showed a weaker green fluorescence compared to controls indicating damaging effects on mitochondria. FDA-PI staining, to determine cell viability, indicated that cells of the trichosetin-treated roots were mostly dead. (C) 2003 Elsevier Science Ltd. All rights reserved.
• Purification and properties of an enzyme capable of degrading the polysaccharide of the cyanobacterium, Nostoc commune

  Dasman; S Kajiyama; A Okazawa; E Fukusaki; A Kobayashi

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 57 (11-12) 1042 - 1046 0939-5075 2002/11 

  A novel Nostoc commune-polysaccharide (NPS)-degrading enzyme with a molecular mass of 128.5 kDa was purified from Paenibacillus glycanilyticus DS-1. The optimum pH and temperature of the enzyme activity were 5.5 and 35degreesC, respectively. The enzyme completely degraded NPS to oligosaccharides, ranging from tetra to hexasaccharides and could degrade the xylan weakly whereas xanthan, gellan, cellulose, curdlan and p-nitrophenyl-beta-D-xylopyra-noside were not degraded. Homology analysis of the N-terminal amino acid sequence of the NPS-degrading enzyme against the PIR and SWISS-PROT databases indicated that the sequence was not homologous to any other polysaccharide-degrading enzyme.
• Paenibacillus glycanilyticus sp nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune

  Dasman; S Kajiyama; H Kawasaki; M Yagi; T Seki; E Fukusaki; A Kobayashi

  INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY SOC GENERAL MICROBIOLOGY 52 (5) 1669 - 1674 1466-5026 2002/09 

  A novel bacterial strain, DS-1(T), was isolated that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune. The isolate was identified by a combination of phenotypic characterization, cellular fatty acid analysis, DNA base composition, DNA-DNA hybridization and 16S rRNA gene sequence analysis. Phylogenetic analysis placed strain DS-1(T) within the Paenibacillus cluster on a phylogenetic tree and the phenotypic characteristics of this strain appear to be similar to those of Paenibacillus curdlanolyticus IFO 15724(T) and Paenibacillus kobensis IFO 15729(T). The strain was distinguished from A curdlanolyticus IFO 15724(T) and A kobensis IFO 15729(T) by its ability to degrade the polysaccharide of Nostoc commune, by assimilation of rhamnose, inositol and L-fucose and by its wide range of optimal growth temperature (28-37degreesC). Like other Paenibacillus species, this strain contains anteiso-C-15:0 as a major cellular fatty acid, and it has a DNA G+C content of 50.5 mol %. Based on these results, it is concluded that this isolate should be placed within a novel species of Paenibacillus, Paenibacillus glycanilyticus sp. nov., with the type strain DS-1(T)(=IFO 16618(T)=JCM 11221(T)=NRRL B-23455(T)).
• Biosynthetic studies of the tetramic acid antibiotic trichosetin

  EC Marfori; T Bamba; S Kajiyama; E Fukusaki; A Kobayashi

  TETRAHEDRON PERGAMON-ELSEVIER SCIENCE LTD 58 (33) 6655 - 6658 0040-4020 2002/08 

  A feeding experiment with C-13-labeled precursors was conducted in the dual cultures of Trichoderma harzianum H14 and Catharanthus roseus callus to determine the biosynthetic origin of the carbon atoms in trichosetin. Results showed that trichosetin originated from two separate biogenetic units. One unit is an octaketide intermediate directly derived from eight intact acetate units joined in a head-to-tail fashion of a polyketide pathway. The other unit is serine. (C) 2002 Elsevier Science Ltd. All rights reserved.
• A novel gene delivery system in plants with calcium alginate micro-beads

  T Sone; E Nagamori; T Ikeuchi; A Mizukami; Y Takakura; SI Kajiyama; EI Fukusaki; S Harashima; A Kobayashi; K Fukui

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 94 (1) 87 - 91 1389-1723 2002/07 

  We have produced micrometer-sized calcium alginate beads referred to as "bio-beads" that encapsulate plasmid DNA molecules carrying a reporter gene. In order to evaluate the efficiency of the bio-beads in mediating genetic transfection, protoplasts isolated from cultured tobacco cells (BY-2) were transfected with bio-beads containing a plasmid that carries the modified green fluorescent protein gene CaMV35S-sGFA With the bio-beads treatment, approximately ten-fold higher GFP expression was observed after 24 It incubation compared to that with the conventional method using a naked plasmid solution. Transfection was up to 0.22% efficient. These results indicate that bio-beads have a possibility for efficient transformation in plants.
• Chromosome mediated genetic transfer by using micro-beads.

  Sone, T; Ikeuchi, T; Mizukami, A; Kajiyama, S; Fukusaki, E; Harashima, S; Kobayashi, A; Fukui, K

  Advances in Chromosome Sciences Chinese Academic Publishers, Beijin 2002/06
• Trichosetin, a novel tetramic acid antibiotic produced in dual culture of Trichoderma harzianum and Catharanthus roseus Callus

  EC Marfori; S Kajiyama; E Fukusaki; A Kobayashi

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 57 (5-6) 465 - 470 0939-5075 2002/05 

  The dual culture of Trichoderma harzianum and Catharanthus roseus callus produced an antimicrobial compound with a remarkable activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis. Structural elucidation revealed that this compound, which we have named trichosetin, is a novel tetramic acid (2,4-pyrrolidinedione) antibiotic and a homolog of the fungal metabolite equisetin. This compound however, was not produced in the individual culture of T. harzianum or C roseus callus.
• Effect of capsaicin and N-docosahexaenoyl-vanillylamide on growth of taxol-tolerant HeLa cells

  Jin Yongfu; Ishihata Kimie; Kajiyama Shin-ichiro; Fukusaki Ei-ichiro; Kobayashi Akio; Baba Naomichi; Tada Mikiro; Takahata Kyoya

  Japanese Journal of Food Chemistry and Safety Japanese Society of Food Chemistry 9 (2) 50 - 53 1341-2094 2002 

  There are few effective clinical studies to inhibit the growth of multidrug resistance tumor cells. We have been interested in the physiological actions of capsaicin (CAP), the pungent ingredient in hot chilli peppers, and polyunsaturated fatty acids, for example docosahexaenoic acid (DHA), extracted from fish oil. In this study, we synthesized a new vanillylamide derivative, N-docosahexaenoyl-vanillylamide (dohevanil), to investigate the inhibitory effect of dohevanil on growth of HeLa cells and taxol-tolerant HeLa cells. As a result, dohevanil has more potent inhibitory effect than CAP for both taxol-sensitive HeLa cells and taxol-tolerant HeLa cells. Particularly, the simultaneous addition of dohevanil and taxol more strongly induced cell death of taxol-tolerant HeLa cells. There results obtained in this study suggest that dohevanil has stronger inhibitory effect than CAP for the multidrug resistance cells.
• SELEX for tubulin affords specific T-rich DNA aptamers

  FUKUSAKI Ei-ichiro; HASUNMA Tomohisa; KAJIYAMA Shin-ichiro; OKAZAWA Atsushi; ITOH J Tomohiko; KOBAYASHI Akio

  Bioorganic & Medicinal Chemistry Letters Vol. 11, No. 22, pp. 2927-2930 2001/11 [Refereed]
  
• Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis

  E Fukusaki; T Oishi; H Tanaka; S Kajiyama; A Kobayashi

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 56 (9-10) 814 - 819 0939-5075 2001/09 [Refereed]
   

  The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.
• The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides Oliver

  T Bamba; E Fukusaki; S Kajiyama; K Ute; T Kitayama; A Kobayashi

  LIPIDS AMER OIL CHEMISTS SOC A O C S PRESS 36 (7) 727 - 732 0024-4201 2001/07 [Refereed]
   

  The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. The presence of long-chain poly-trans prenols (> 9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.
• High-resolution analysis of polyprenols by supercritical fluid chromatography

  T Bamba; E Fukusaki; S Kajiyama; K Ute; T Kitayama; A Kobayashi

  JOURNAL OF CHROMATOGRAPHY A ELSEVIER SCIENCE BV 911 (1) 113 - 117 0021-9673 2001/03 [Refereed]
   

  A high-resolution analysis of polyprenol mixtures was achieved by supercritical fluid chromatography (SFC). The separation of polyprenols was examined on an octadecylsilane-packed column with liquid carbon dioxide as the mobile phase and ethanol as modifier. Using this chromatography system, the resolution of separation (R-s) between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) was two times higher than that using conventional reversed-phase highperformance liquid chromatography. Our SFC technique allows the advantage of baseline separation of polyprenol samples containing hydrophobic components such as terpenes or fatty acids that are unfavorable for good separation. This method is very useful for the analysis of structurally close polyprenol analogues of rubber plant metabolites. (C) 2001 Elsevier Science B.V. All rights reserved.
• Taxezopidines J, K, and L, new taxoids from Taxus cuspidata inhibiting Ca2+-induced depolymerization of microtubules

  H Shigemori; CA Sakurai; H Hosoyama; A Kobayashi; S Kajiyama; J Kobayashi

  TETRAHEDRON PERGAMON-ELSEVIER SCIENCE LTD 55 (9) 2553 - 2558 0040-4020 1999/02 

  Three new taxoids, taxezopidines J (1), K (2), and L (3), have been isolated from seeds of the Japanese yew Taxus cuspidata Sieb. et Zucc. and the structures were elucidated on the basis of spectroscopic data and chemical means. Taxezopidines K (2) and L (3) markedly inhibited Ca2+-induced depolymerization of microtubules, while taxuspine D (4), a known taxoid from the yew, induced unusual shape change of mitotic spindles like taxol. (C) 1999 Elsevier Science Ltd. Ail rights reserved.
• Nostofungicidine, an antifungal lipopeptide from the field-grown terrestrial blue-green alga Nostoc commune

  S Kajiyama; H Kanzaki; K Kawazu; A Kobayashi

  TETRAHEDRON LETTERS PERGAMON-ELSEVIER SCIENCE LTD 39 (22) 3737 - 3740 0040-4039 1998/05 

  In the course of our screening program for bioactive compounds, a novel lipopeptide, nostofungicidine (1), was isolated from the methanolic extract of a field-grown terrestrial blue-green alga, Nostoc commune. The structure of nostofungicidine was elucidated by chemical degradation and extensive NMR measurements including DQF-COSY, HOHAHA, HMBC, and ROESY techniques. Nostofungicidine contains a novel beta-amino acid 3-amino-6-hydroxy stearic acid (Ahs) in its structure. (C) 1998 Elsevier Science Ltd. All rights reserved.
• Secondary metabolites of cyanobacteria Nostoc sp.

  Chin. J. Oceanol. Limnol. 16 (Suppliment) 109 - 117 1998
• Antimicrobial constituents of Angelica dahurica roots

  YS Kwon; A Kobayashi; SI Kajiyama; K Kawazu; H Kanzaki; CM Kim

  PHYTOCHEMISTRY PERGAMON-ELSEVIER SCIENCE LTD 44 (5) 887 - 889 0031-9422 1997/03 

  One novel coumarin from Angelica dahurica roots was elucidated to be 5,8-di(2,3-dihydroxy-3-methylbutoxy)-psoralen. It occurs together with six other known coumarins and ferulic acid. The antimicrobial activity of the coumarins and ferulic acid were compared. Copyright (C) 1997 Elsevier Science Ltd.
• Tectona grandis Callus Produces Antibacterial Triterpene Acids not Detected in the Intact plant

  MARWANI E.

  Natural Product Sciences 3 (1) 75 - 80 1997
• N-linked sugar chain of 55-kDa royal jelly glycoprotein

  Y Kimura; S Kajiyama; J Kanaeda; T Izukawa; M Yonekura

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 60 (12) 2099 - 2102 0916-8451 1996/12 

  An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci, Biotech. Biochem., 59, 507-509 (1995)], has been identified, The sugar chains mere released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential eso-mannosidase digestions, MALDI-TOF MS, and 500 MHz H-1-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Man alpha 1-->2Man alpha 1-->6(Man alpha 1-->2Man alpha 1-->3)Man alpha 1-->6(Man alpha 1-->2Man alpha 1-->2Man alpha 1-->3)Man beta 1-->4GlcNAc beta 1-->4GlcNAc, which is a non-processed high mannose type structure.
• A cytotoxic principle of Tamarindus indica, di-n-butyl malate and the structure-activity relationship of its analogues

  A Kobayashi; MI Adenan; SI Kajiyama; H Kanzaki; K Kawazu

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 51 (3-4) 233 - 242 0939-5075 1996/03 

  Bioassay-guided fractionation of the methanolic extract of Tamarindus indica fruits led to the isolation of L-(-)-di-n-butyl malate which exhibited a pronounced cytotoxic activity against sea urchin embryo cells. In order to study structure-activity relationships, close-structure relatives of di-n-butyl malate were synthesized using D-(+)- and L-(-)-malic acid as starting materials, and their cytotoxic activities were examined for the sea urchin embryo assay. L-(-)-Di-n-pentyl malate was the most effective inhibitor to the development of the fertilized eggs. Significant inhibitory activity was not seen in the esters of D-(-)-isomer.
• ANTIFUNGAL COMPOUNDS INDUCED IN THE DUAL CULTURE WITH PHYTOLACCA-AMERICANA CALLUS AND BOTRYTIS-FABAE

  A KOBAYASHI; K HAGIHARA; S KAJIYAMA; H KANZAKI; K KAWAZU

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 50 (5-6) 398 - 402 0939-5075 1995/05 

  In order to investigate new metabolites which are only induced in a plant callus infected by a pathogenic fungus, dual cultures with combinations of 10 species of fungi and 6 plant cell lines from different species were established. Among the combinations tested, the methanolic extract of a dual culture consisting of a plant cell line, Phytolacca americana and a fungus, Botrytis fabae showed a marked antifungal activity to Cladosporium herbarum. The main active constituent of this extract was identified to be phytolaccoside B (Pls B) by the spectroscopic analyses.
• ANTIFEEDANT AND ANTIOVIPOSITION ACTIVITIES OF THE FRACTIONS OF EXTRACT FROM A TROPICAL PLANT, ANDROGRAPHIS-PANICULATA (ACANTHACEAE), AGAINST THE DIAMONDBACK MOTH, PLUTELLA-XYLOSTELLA (LEPIDOPTERA, YPONOMEUTIDAE)

  W HERMAWAN; S KAJIYAMA; R TSUKUDA; K FUJISAKI; A KOBAYASHI; F NAKASUJI

  APPLIED ENTOMOLOGY AND ZOOLOGY JAPAN SOC APPL ENTOMOL ZOOL 29 (4) 533 - 538 0003-6862 1994/11 

  Antifeedant activity of extracts from Andrographis paniculata against the 1st and the 4th-instar larvae of the diamondback moth (DBM), Plutella xylostella, was tested by using leaf disc of cabbage. Furthermore, the antioviposition effect was also observed on the caged cabbage plant treated with the fractions. The active fractions from the acetone extract suppressed feeding by the 1st and 4th-instar larvae at the concentrations of 60 ppm and 500 ppm, respectively. The antioviposition activity against DBM females was also found at the concentration of 125 ppm.
• A NEW-TYPE OF ANTIMICROBIAL PHENOLICS PRODUCED BY PLANT PEROXIDASE AND ITS POSSIBLE ROLE IN THE CHEMICAL DEFENSE SYSTEMS AGAINST PLANT-PATHOGENS

  A KOBAYASHI; Y KOGUCHI; H KANZAKI; SI KAJIYAMA; K KAWAZU

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 49 (7-8) 411 - 414 0939-5075 1994/07 

  Syringaldehyde readily reacted in the horse-radish peroxidase (HRPOD) system. The ethyl acetate extract of the reaction mixture showed a marked antimicrobial activity against bacteria and fungi. After repeated column chromatography three potential antimicrobial compounds were obtained from the extract. The structural elucidation of active compounds was achieved by a combination of spectroscopic techniques and chemical modification.
• NOSTODIONE-A, A NOVEL MITOTIC SPINDLE POISON FROM A BLUE-GREEN-ALGA NOSTOC-COMMUNE

  A KOBAYASHI; SI KAJIYAMA; K INAWAKA; H KANZAKI; K KAWAZU

  ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES VERLAG Z NATURFORSCH 49 (7-8) 464 - 470 0939-5075 1994/07 

  A novel antimitotic compound named Nostodione A (Nd A) was isolated from a terrestrial blue-green alga Nostoc commune. Nostodione A disturbed the mitotic spindle formation of sea-urchin eggs and gave small spindles with low birefringence density. Nostodione A, however, had no phytotoxicity on the germination of a dicotyledonous plant Medicago sativa. Based on the spectral analysis and chemical degradation, the structure of Nostodione A was elucidated.
• NEW TELEOCIDIN-RELATED METABOLITES, (-)-7-GERANYLINDOLACTAM-V AND BLASTMYCETIN-F, FROM STREPTOVERTICILLIUM-BLASTMYCETICUM

  K IRIE; SI KAJIYAMA; S OKUNO; M KONDO; K KOSHIMIZU; H HAYASHI; M ARAI; H NISHINO; A IWASHIMA

  JOURNAL OF NATURAL PRODUCTS AMER SOC PHARMACOGNOSY 57 (3) 363 - 368 0163-3864 1994/03 

  Two new teleocidin-related metabolites, (-)-7-geranylindolactam-V [2] and blastmycetin F [3], were isolated from fermentation broths of the actinomycete Streptoverticillium blastmyceticum NA34-17, and their structures were determined by spectroscopic methods. Compound 2 bound strongly to phorbol ester receptors in a mouse epidermal particulate fraction, suggesting that it is a potent in vivo rumor promoter comparable to teleocidins A-1 [4] and B-4 [5].
• PRODUCTION OF A NEW-TYPE OF BIOACTIVE PHENOLIC COMPOUND

  A KOBAYASHI; Y KOGUCHI; H KANZAKI; S KAJIYAMA; K KAWAZU

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 58 (1) 133 - 134 0916-8451 1994/01 

  Peroxidase (POD) catalyzed the polymerization of naturally occurring phenolics to give a variety of bioactive products. The guaiacol readily reacted in the POD system and afforded potential anti-microbial compounds. Three active phenols, 1, 2, and 3, were isolated from the reaction mixture. Spectroscopic analyses elucidated that 1 and 2 were a dimer and a trimer respectively originating from guaiacol. Compound 3 was readily oxidized to 4 by exposure to air. The structure of 4 was determined by a combination of chemical modification and spectroscopic analyses, and 3 was elucidated to be the hydroxyquinone form of 4.
• Quantitative structure-activity studies on indole alkaloid tumor promoter indolactam congeners

  Kazuhiro Irie; Shigenori Okuno; Shin ichiro Kajiyama; Koichi Koshimizu; Hoyoku Nishino; Akio Iwashima

  Carcinogenesis 12 (10) 1883 - 1886 0143-3334 1991/10 [Refereed]
   

  Nine indolactam congeners with L-Abu, γ,δ-δ-Nva, Nva, Phg, tert-Leu, Leu, Ile, allo-Ile, Nle, instead of L-Val In (-)-indolactam-Val, were synthesized by microbial conversion and examined using two biological tests related to tumor promotion. The tests were inhibition of the specific binding of [3H]12-O-tetradecanoylphorbol-13-acetate (TPA) to the mouse epidermal particulate fraction, and stimulation of radioactive inorganic phosphate incorporation into phospholipids of HeLa cells. These two biological activities correlated well for each derivative. (-)-Indolactam-Ile with L-Ile in place of L-Val in (-)-indolactam-Val, which we have recently confirmed to occur naturally, was the most active of the ten indolactam congeners tested, suggesting that (-)-indolactam Ile is a more potent tumor promoter than (-)-indolactam Val in vivo. The effects of the substituents at position 12 on the TPA receptor binding were analyzed quantitatively using physicochemical substituent parameters and regression analysis. The results indicated that both hydrophobicity and bulkiness of the substituents at position 12 increased the binding ability to the TPA receptor, supporting the recent hypothesis that the isopropyl group at position 12 of (-)-indolactam-Val is involved in the hydrophobic interaction on the receptor site. © 1991 Oxford University Press.
• SYNTHESIS OF NEW INDOLACTAM ANALOGS BY MICROBIAL CONVERSION

  S KAJIYAMA; K IRIE; T KIDO; K KOSHIMIZU; H HAYASHI; M ARAI

  TETRAHEDRON PERGAMON-ELSEVIER SCIENCE LTD 47 (29) 5453 - 5462 0040-4020 1991/07 

  Ten indolactam congeners with L-Ala, Abu, gamma, delta-DELTA-Nva, Nva, Nle, tert-Leu, Leu, Ile, allo-Ile, allo-Ile, Phg instead of L-Val in (-)-indolactam-Val, were synthesized from their seco-compounds (N-methyl-L-amino acidyl-L-tryptophanol) by microbial conversion.
• ISOLATION AND BIOSYNTHESIS OF (-)-INDOLACTAM-I, A NEW CONGENER OF INDOLE ALKALOID TUMOR PROMOTER TELEOCIDINS

  K IRIE; S KAJIYAMA; K KOSHIMIZU; H HAYASHI; M ARAI

  TETRAHEDRON LETTERS PERGAMON-ELSEVIER SCIENCE LTD 31 (50) 7337 - 7340 0040-4039 1990
• BIOSYNTHESIS OF (-)-INDOLACTAM-V, THE BASIC RING-STRUCTURE OF TUMOR PROMOTERS TELEOCIDINS

  K IRIE; S KAJIYAMA; A FUNAKI; K KOSHIMIZU; H HAYASHI; M ARAI

  TETRAHEDRON LETTERS PERGAMON-ELSEVIER SCIENCE LTD 31 (1) 101 - 104 0040-4039 1990
• Biosynthesis of indole alkaloid tumor promoters teleocidins (I) possible biosynthetic pathway of the monoterpenoid moieties of teleocidins

  Kazuhiro Irie; Shin ichiro Kajiyama; Atsushi Funaki; Koichi Koshimizu; Hideo Hayashi; Motoo Arai

  Tetrahedron 46 (8) 2773 - 2788 0040-4020 1990 [Refereed]
   

  Possible biosynthetic pathway of the monoterpenoid moieties of teleocidins shown in Fig. 8 was proposed by feeding experiments with several D or 13C-labelled precursors and isolation of new telecidin-related metabolites named blastmycetin D (6) and E (7). © 1990.

Books etc

• Citrus Pathology

  etsuya Matsukawa; Tomonori Asai and (Joint workChapter 3：Metabolic Changes during Defense Responses against)InTech 2017/04 9789535130727
• Transgenic Plants - Advances and Limitations

  Naruemon Khemkladngoen; Naoki Wada; Suguru Tsuchimoto; Joyce A. Cartagena; Shin-ichiro Kajiyama; Kiichi Fukui (Joint workChapter 5 Bioactive Beads-Mediated Transformation of Rice with Large DNA Fragments Containing Aegilops tauschii Genes, with Special Reference to Bead-Production Methodology)InTech 2012/09 9789535101819
• Plant Transformation Technologies

  N. Wada; S. Kajiyama; N. Khemkladngoen; K. Fukui (Joint workChapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads)Wiley-Blackwell 2011/08 0813821959
• キャンパスに咲く花

  梶山 慎一郎 (Joint work)大阪大学出版会 2008
• ナノイメージング(分担）

  ＮＴＳ 2008
• ナノバイオ大辞典

  テクノシステム 2007

Conference Activities & Talks

• Jatropha curcasの種子採油残渣を用いた微生物農薬の開発  [Not invited]

  吉川 諒; 大池 達矢; 松川 哲也; 岡南 政宏; 梶山 慎一郎; 阿野 貴司

  日本農芸化学会2012年度大会  2012/03  日本農芸化学会2012年度大会
  
• ジャトロファフォルボールエステル分解微生物の探索  [Not invited]

  大池 達矢; 松川 哲也; 岡南 政宏; 梶山 慎一郎; 阿野 貴司

  日本農芸化学会2012年度大会  2012/03  日本農芸化学会2012年度大会
  
• Isolation of phorbol ester degrading microbes for Jatropha bio-diesel production.  [Not invited]

  大池 達矢; 三倉 健; 赤井 一樹; 松川 哲也; 岡南 政宏; 梶山 慎一郎; 阿野 貴司

  International Union of Microbial Societies 2011  2011/09  International Union of Microbial Societies 2011
  
• ジャトロファ種子1粒を用いた脂質およびフォルボールエステル類の分析  [Not invited]

  松川 哲也; 岡南 政宏; 阿野 貴司; 梶山 慎一郎

  2010/03
• ジャトロファを原料としたバイオディーゼル生産工程で排出されるフォルボールエステル類の定量分析  [Not invited]

  松川 哲也; 梶山 慎一郎

  2009/09
• モモ葉におけるジャスモンサン誘導性物質の同定  [Not invited]

  松川 哲也; 梶山 慎一郎; 石原 亨

  2009/03
• フェムト秒レーザーを用いたＧＡＢＡ高生産酵母の育種  [Not invited]

  堤 浩子; 梶山 慎一郎; 犬童 雅栄; 坂倉 政明; 橋本 卓哉; 福崎 英一郎; 植田 充美; 平尾 一之; 秦 洋二; 安部 康久

  日本農芸化学会 2009年度大会  2009  日本農芸化学会 2009年度大会
  
• コムギ硬軟質に関わるfriabilin関連遺伝子群のイネへの導入と蓄積  [Not invited]

  鈴木剛; 梶山 慎一郎; 和田幸雄; 和田英雄; 後藤公美; 中野亜紀子; 和田直樹; 福井希一; 向井康比己

  日本育種学会第116回講演会  2009  日本育種学会第116回講演会
  
• エンバク感染応答の１細胞分析  [Not invited]

  梶山 慎一郎; 和泉 自泰; 中村 亮介; 石原 亨; 岡澤 敦司; 福崎 英一郎; 兼松 泰男; 小林 昭雄

  日本生物工学会2009年大会シンポジウム  2009  日本生物工学会2009年大会シンポジウム
  
• ナノフロー液体クロマトグラフィー/質量分析計による植物代謝産物の高感度定量分析  [Not invited]

  和泉 自泰; 梶山 慎一郎; 岡澤 敦司; 馬場 健史; 小林 昭雄; 福崎 英一郎

  第4回メタボロームシンポジウム  2009  第4回メタボロームシンポジウム
  
• 極短パルスレーザーの生物科学への応用  [Not invited]

  梶山 慎一郎; 植田充美; 平尾一之; 福井希一; 伊東一良

  集積光デバイス研究会2008  2008  集積光デバイス研究会2008
  
• Stimulated parametric emission microscopy for high-resolution 3-D imaging of unstained live-cell  [Not invited]

  Hieu M.Dang; 梶山 慎一郎; Takehito Kawasumi; Yasuyuki Ozeki; Kazuyoshi Itoh; Kiichi Fukui

  3次元画像コンファレンス2008  2008  3次元画像コンファレンス2008
  
• レーザーアブレーションを用いた疎水性生理活性物質の水中分散法の検討  [Not invited]

  深田 洋輔; 梶山 慎一郎; 朝日 剛; 安國 良平; 福井 希一

  日本生物工学会2008年大会  2008  日本生物工学会2008年大会
  
• フェムト秒レーザーを用いたGABA高生産酵母の育種  [Not invited]

  堤 浩子; 梶山 慎一郎; 水本 真紀子; 犬童 雅栄; 坂倉 政明; 橋本 卓哉; 福崎 英一郎; 植田 充美平尾; 秦 洋二

  日本生物工学会2008年大会  2008  日本生物工学会2008年大会
  
• Development of high speed screening system for enzyme surface displaying yeast by flow sorting  [Not invited]

  Yan ZHOU; 梶山 慎一郎; Kouichi ITOH; Takanori TANINO

  The Society for Biotechnology Japan, Annual Meeting 2008  2008  The Society for Biotechnology Japan, Annual Meeting 2008
  
• バイオアクティブビーズ法によるハードネス遺伝子群導入イネの作出  [Not invited]

  和田直樹; 梶山 慎一郎; 秋山征夫; 大谷基泰; 島田多喜子; 鈴木剛; 向井康比己; 福井希一

  日本育種学会第114回講演会  2008  日本育種学会第114回講演会
  
• ライフサイエンス研究に資する無染色可視化顕微鏡の開発  [Not invited]

  梶山 慎一郎

  大阪大学フロンティア先端研究フォーラム  2007  大阪大学フロンティア先端研究フォーラム
  
• A novel gene delivery system in plants with calcium alginate micro-beads  [Not invited]

  Wada, N; 梶山 慎一郎; Fukui, K

  International Conference on Plant Transformation Technologies  2007  International Conference on Plant Transformation Technologies
  
• シングルセル代謝分析に基づいた植物感染応答反応の動的解析  [Not invited]

  和泉 自泰; 梶山 慎一郎; 福崎 英一郎; 兼松 泰男; 中村 亮介; 石原 亨; 小林 昭雄

  日本農芸化学会 2007年度大会  2007  日本農芸化学会 2007年度大会
  
• 酸化ストレス条件下でのエラープローンなDNA polymerase の発現解析  [Not invited]

  平野 美紀; 梶山 慎一郎; 栗原 佐智子; 福井 希一

  日本農芸化学会 2007年度大会  2007  日本農芸化学会 2007年度大会
  
• 緑の地球の古今の立役者--ランソウから遺伝子改変植物へ  [Not invited]

  小林 昭雄; 梶山 慎一郎; 岡澤 敦司

  日本地衣学会第6回シンポジウム  2007  日本地衣学会第6回シンポジウム
  
• 動植物の組織・細胞単離のためのフェムト秒レーザー加工法  [Not invited]

  坂倉政明; 梶山 慎一郎; 司 金海; 平尾一之; 栃木憲治; 植田充美

  第68回日本応用物理学会  2007  第68回日本応用物理学会
  
• Cell imaging by spectral-interferometric stimulated parametric emission microscopy with enhanced sensitivity and fluorescence separation capability  [Not invited]

  Y. Ozeki; 梶山 慎一郎; K. Isobe; T. Suzuki; K. Fukui; K. Itoh

  3rd Asian and Pacific Rim Conference on Biophotonics  2007  3rd Asian and Pacific Rim Conference on Biophotonics
  
• Single cell sampling and analysis by ultra short pulse laser  [Not invited]

  梶山 慎一郎; Sakakura, M; Ueda, M; Itoh, K; Fukui, K

  5th International forum of post genome technologies  2007  5th International forum of post genome technologies
  
• 植物の機能解析（ゴム代謝器官のイメージング技術開発）  [Not invited]

  梶山 慎一郎; 中澤慶久; 武田強; 馬場健史; 中堂薗陽子; 上藤洋敬; 福崎英一郎; 小林昭雄

  大阪大学先端科学イノベーションセミナー  2007  大阪大学先端科学イノベーションセミナー
  
• 励起・蛍光時間マトリクスと1細胞サンプリングによるエンバク感染応答反応の解析  [Not invited]

  梶山 慎一郎; 和泉 自泰; 石原 亨; 中村 亮介; 兼松 泰男; 福崎 英一郎; 小林 昭雄

  日本農芸化学会 2006年度大会  2006  日本農芸化学会 2006年度大会
  
• Dynamic analysis of plant defence responses based on single-cell metabolite mapping  [Not invited]

  Kobayashi, A; 梶山 慎一郎

  International Symposium on Molecular Nano Dynamics  2006  International Symposium on Molecular Nano Dynamics
  
• Single cell based metabolite analysis upon defense response of oat by laser micro sampling and nano flow LC-MS  [Not invited]

  梶山 慎一郎; Izumi, Y; Fukusaki, E; Kobayashi, A

  The 1st Workshop on Approaches to Single-Cell Analysis  2006  The 1st Workshop on Approaches to Single-Cell Analysis
  
• フェムト秒レーザーを用いた細胞のサンプリングと分析  [Not invited]

  梶山 慎一郎

  分光学会関西支部大会シンポジウム  2006  分光学会関西支部大会シンポジウム
  
• ヒト細胞へのストレス負荷によるエラープローンなDNA polymeraseの発現解析  [Not invited]

  平野 美紀; 梶山 慎一郎; 栗原 佐智子; 秋山 征夫; 福井 希一

  日本生物工学会2006年度大会  2006  日本生物工学会2006年度大会
  
• 1細胞分析に資するレーザーテクノロジー  [Not invited]

  梶山 慎一郎; 小林 昭雄; 福井 希一; 伊東 一良

  日本生物工学会2006年度大会 シンポジウム  2006  日本生物工学会2006年度大会 シンポジウム
  
• 質量分析による転写産物の新規定量法の開発  [Not invited]

  広実 慶彦; 梶山 慎一郎; 福崎 英一郎; 小林 昭雄

  日本生物工学会 2006年度大会  2006  日本生物工学会 2006年度大会
  
• Single-cell based metabolite analyses by laser microsampling and nano-flow LC-MS  [Not invited]

  梶山 慎一郎

  The 4th International forum on post-genome technologies  2006  The 4th International forum on post-genome technologies
  
• 細胞代謝産物解析に資するレーザーテクノロジー  [Not invited]

  梶山 慎一郎

  NEDO光集積ラボ スタートアップセミナー  2006  NEDO光集積ラボ スタートアップセミナー
  
• ナノスプレイヤー一体型カラムを用いた低拡散nano flow LC-ESI-MSによる高感度分析系の確立とその応用  [Not invited]

  福崎英一郎; 梶山 慎一郎; 和泉自泰; 岡澤敦司; 小林昭雄

  大阪大学先端科学イノベーションセミナー  2006  大阪大学先端科学イノベーションセミナー
  
• フォトンクラフト技術を利用した生体適応型分子メスの開発  [Not invited]

  植田充美; 梶山 慎一郎; 坂倉政明

  知的・産業クラスター連携事業 京都バイオ計測プロジェクト第二回講演会  2006  知的・産業クラスター連携事業 京都バイオ計測プロジェクト第二回講演会
  
• フォトンクラフト技術を利用した生体適応型分子メスの開発  [Not invited]

  坂倉政明; 梶山 慎一郎

  地域発先端テクノフェア2006  2006  地域発先端テクノフェア2006
  
• レーザー1細胞サンプリングによるエンバク感染応答反応の解析  [Not invited]

  梶山 慎一郎; 和泉 自泰; 石原 亨; 広実 慶彦; 福崎 英一郎; 小林 昭雄

  日本農芸化学会 2005年度大会  2005  日本農芸化学会 2005年度大会
  
• レーザーアブレーションを用いた植物細胞への遺伝子導入  [Not invited]

  梶山 慎一郎; 井上 文秀; 嶋村 正樹; 広実 慶彦; 福崎 英一郎; 富澤 健一; 小林 昭雄

  日本農芸化学会 2005年度大会  2005  日本農芸化学会 2005年度大会
  
• フォトンクラフト技術を利用した生体適応型分子メスの開発  [Not invited]

  梶山 慎一郎

  第2回 KYO-NANOフォトニックセミナー  2005  第2回 KYO-NANOフォトニックセミナー
  
• レーザー1細胞サンプリングによるエンバクファイトアレキシンの分析  [Not invited]

  梶山 慎一郎; Benesh Joseph; 井上文秀; 嶋村正樹; 福崎英一郎; 富澤健一; 小林昭雄

  日本農芸化学会関西支部・中四国支部・合同大会  2005  日本農芸化学会関西支部・中四国支部・合同大会
  
• トランス型ゴム産生植物トチュウEucommia ulmoidesのEST解析  [Not invited]

  梶山 慎一郎; 福崎英一郎; 小林昭雄; 中澤慶久; 馬場健史; 西河貴司; 山下敦士; 服部正平; 小笠原直毅

  大阪大学先端科学イノベーションセミナー  2005  大阪大学先端科学イノベーションセミナー
  
• ESI-MSを用いた転写産物の分析（I）―イオン化条件の検討―  [Not invited]

  梶山 慎一郎; 梨原 善行; 和泉 自泰; 原田 和生; 福崎 英一郎; 小林 昭雄

  日本生物工学会2005年度大会  2005  日本生物工学会2005年度大会
  
• レーザ微細加工による細胞操作  [Not invited]

  梶山 慎一郎

  第3回Round-Robin Seminar  2005  第3回Round-Robin Seminar
  

MISC

• 覧古考新

  梶山 慎一郎  生物工学会誌  101-  (7)  333  -333  2023/07
• Profiling of leaf volatiles of 17 mango cultivars using SPME-GC/MS

  藤阪芽以; 松川哲也; 松川哲也; 志水恒介; 伊藤仁久; 重岡成; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2022-  2022
• Evaluation of apigenin as a sperm chemoattractant in the liverwort Marchantia polymorpha by targeted disruption of the chalcone isomerase gene

  武部夏実; 諏訪宏紀; 松川哲也; 大和勝幸; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2022-  2022
• バイオ系のキャリアデザイン 大学教員を考えている皆さんへ

  梶山 慎一郎  Seibutsu-kogaku Kaishi  99-  (10)  547  -551  2021/10
• Inhibitory effects of Calophyllum inophyllum seed oil on matrix metalloproteinase 9

  松井杏美; 松川哲也; 宇佐美徹; 梶山慎一郎  日本生物工学会大会講演要旨集  73rd-  2021
• Characterization of Marchantia polymorpha L. chalcone synthase knockout mutant on sperm-attraction

  諏訪宏紀; 武部夏実; 山崎由美子; 松川哲也; 大和勝幸; 梶山慎一郎  日本生物工学会大会講演要旨集  73rd-  2021
• 〈Research Notes〉An objective evaluation of Lilium japonicum's aroma effects on prefrontal lobe using fNIRS.

  松本知樹; 小濱剛; 松川哲也; 瀧川義浩; 吉田久; 梶山慎一郎  Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University  (43)  53  -61  2020/03
• 〈Research Notes〉Screening of antifungal volatile compounds derived from microorganisms

  大池達矢; 江邉正平; 松川哲也; 岡南政宏; 梶山慎一郎; 阿野貴司  Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University  (43)  63  -72  2020/03
• Identification of a sperm chemoattractant in the liverwort Marchantia polymorpha L.

  山崎由美子; 松川哲也; 竹村美保; 荒木崇; 大和勝幸; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2020-  2020
• Aroma effects on hemodynamic modulation in the prefrontal lobe based on an fNIRS measurement

  松本知樹; 小濱剛; 松川哲也; 滝川義浩; 梶山慎一郎  映像情報メディア学会技術報告  43-  (37)  33  -36  2019/11
• Jatropha curcas種子に含まれる二次代謝物質の網羅的解析

  松久保康輔; 松川哲也; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2019-  ROMBUNNO.3E7p05 (WEB ONLY)  2019/03
• 花香分析とRAPD解析によるササユリ(Lilium japonicum)系統間の多様性解析

  藤澤瑞希; 荒井滋; 松川哲也; 瀧川義浩; 秋野順治; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2019-  ROMBUNNO.4D2a03 (WEB ONLY)  2019/03
• ゼニゴケ(Marchantia polymorpha)の精子誘引物質の探索

  山崎由美子; 竹村美保; 松川哲也; 大和勝幸; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2019-  ROMBUNNO.4D5a10 (WEB ONLY)  2019/03
• ササユリ花香成分のポリネーターに対する誘引

  花崎直史; 秋野順治; 荒井滋; 松川哲也; 瀧川義浩; 梶山慎一郎  日本生態学会大会講演要旨(Web)  66th-  ROMBUNNO.P1‐322 (WEB ONLY)  2019/02
• Promotion of lipopeptide antibiotic production by Bacillus sp. IA in the presence of rice husk biochar

  Ebe Shohei; Ohike Tatsuya; Matsukawa Tetsuya; Okanami Masahiro; Kajiyama Shin'ichiro; Ano Takashi  Journal of Pesticide Science  44-  (1)  33  -40  2019
• ササユリ(Lilium japonicum)系統間および類縁種間の花香組成を指標とした化学分類の試み

  藤澤瑞希; 松川哲也; 瀧川義浩; 荒井滋; 秋野順治; 柳川綾; 梶山慎一郎  日本生物工学会大会講演要旨集  70th-  163  2018/08
• 放線菌由来揮発性有機化合物が植物病原菌に与える影響

  前田みのり; 大池達矢; 岡南政宏; 松川哲也; 梶山慎一郎; 阿野貴司  日本生物工学会大会講演要旨集  70th-  165  2018/08
• 在来カンキツのストレス応答反応における二次代謝物質のプロファイリング

  伊佐木芳; 松川哲也; 松川哲也; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2018-  ROMBUNNO.2A29a03 (WEB ONLY)  2018/03
• CRISPR/Cas9を用いたJatropha curcas L.のゲノム編集の試み

  藤波友樹; 児玉岳; 十川太輔; 松川哲也; 大和勝幸; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2018-  ROMBUNNO.3A28a13 (WEB ONLY)  2018/03
• ササユリ(Lilium japonicum)の二次代謝産物分析

  藤澤瑞希; 松川哲也; 瀧川義浩; 荒井滋; 柳川綾; 秋野順治; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2018-  ROMBUNNO.2A17a10 (WEB ONLY)  2018/03
• 未利用農産資源の機能性素材としての開発研究―ウンシュウミカン花のリパーゼ阻害作用―

  伊藤仁久; 村田和也; 山上桃奈; 松川哲也; 松川哲也; 梶山慎一郎; 文室政彦; 飯嶋盛雄; 飯嶋盛雄; 松田秀秋; 松田秀秋  日本薬学会年会要旨集(CD-ROM)  138th-  ROMBUNNO.27PA‐pm242  2018
• Jatropha curcasの産生するホルボールエステル類の生理活性評価

  柳内知哉; 小田垣瑞穂; 高田果歩; 松川哲也; 西海史子; 名倉由起子; 柳原格; 梶山慎一郎  日本生物工学会大会講演要旨集  69th-  258  2017/08
• 種々のストレス状況下におけるカンキツ類の二次代謝物質のプロファイリング

  伊佐木芳; 松川哲也; 松川哲也; 梶山慎一郎  日本生物工学会大会講演要旨集  69th-  258  2017/08
• 放線菌由来生理活性物質が植物および植物病原菌に与える影響

  前田みのり; 大池達矢; 岡南政宏; 松川哲也; 梶山慎一郎; 阿野貴司  日本生物工学会大会講演要旨集  69th-  321  2017/08
• ブンタン葉のストレス応答反応における二次代謝物質の変動解析

  伊佐木芳; 松川哲也; 松川哲也; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2017-  ROMBUNNO.2J31a01 (WEB ONLY)  2017/03
• 微生物が生産する揮発性有機化合物の農業利用に向けた基礎的研究

  米田奈央; 江邉正平; 大池達矢; 松川哲也; 岡南政宏; 梶山慎一郎; 阿野貴司  日本微生物生態学会大会(Web)  2017-  ROMBUNNO.P‐154 (WEB ONLY)  2017
• 三枝祭で奉られるササユリの花香が示す抗菌性

  花崎直史; 秋野順治; 柳川綾; 梶山慎一郎; 瀧川義浩; 松川哲也; 荒井滋  日本生態学会大会講演要旨(Web)  64th-  ROMBUNNO.P1‐B‐084 (WEB ONLY)  2017
• 未利用農産資源の機能性素材としての開発研究―マンゴー葉のリパーゼ阻害作用―

  伊藤仁久; 村田和也; 中垣友太; 清水彩加; 高田雄輔; 志水恒介; 松川哲也; 松川哲也; 梶山慎一郎; 文室政彦; 飯嶋盛雄; 飯嶋盛雄; 松田秀秋; 松田秀秋  日本薬学会年会要旨集(CD-ROM)  137th-  ROMBUNNO.26PA‐am026  2017
• アルボサイクリン生産放線菌を用いた微生物農薬の開発

  大池達矢; 前田みのり; 松川哲也; 松川哲也; 岡南政宏; 岡南政宏; 梶山慎一郎; 梶山慎一郎; 阿野貴司; 阿野貴司  日本生物工学会大会講演要旨集  68th-  243  2016/08
• ハッサク葉のストレス応答反応における二次代謝物質の変動解析

  伊佐木芳; 松川哲也; 松川哲也; 梶山慎一郎  日本生物工学会大会講演要旨集  68th-  300  2016/08
• Streptomyces sp.KT株の微生物農薬としての可能性

  前田みのり; 大池達矢; 岡南政宏; 岡南政宏; 松川哲也; 松川哲也; 梶山慎一郎; 梶山慎一郎; 阿野貴司; 阿野貴司  日本生物工学会大会講演要旨集  68th-  243  2016/08
• 灌漑用水条件の異なるエジプト産ホホバ種子中の代謝物の変動解析

  藤波友樹; 松川哲也; HEGAZY Adel; 梶山慎一郎  日本生物工学会大会講演要旨集  68th-  123  2016/08
• 御神花ササユリの葉には抗菌性がある

  岩佐郁; 秋野順治; 梶山慎一郎; 瀧川義浩; 松川哲也; 柳川綾; 荒井滋  日本応用動物昆虫学会大会講演要旨  60th-  187  2016/03
• ウメの品種間代謝物プロファイリング

  松川哲也; 平山幸千; 北村祐人; 竹中正好; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2016-  2H006 (WEB ONLY)  2016/03
• カンキツ類の傷害応答反応における二次代謝物質の変動解析

  伊佐木芳; 松川哲也; 浅井智紀; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2016-  2H001 (WEB ONLY)  2016/03
• カンキツ葉中の一次代謝物質の環境ストレス応答における代謝変動解析

  浅井智紀; 松川哲也; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2016-  2H002 (WEB ONLY)  2016/03
• 三輪明神摂社三枝祭の御神花ササユリの花香とリーフワックスの機能

  岩佐郁; 近藤陽香; 秋野順治; 柳川綾; 梶山慎一郎; 瀧川義浩; 荒井滋  日本生態学会大会講演要旨(Web)  63th-  P2‐190 (WEB ONLY)  2016
• 放線菌由来抗菌活性物質の探索

  UETSUKI YOSHIHIRO; OIKE TATSUYA; MATSUKAWA TETSUYA; OKANAMI MASAHIRO; KAJIYAMA SHIN'ICHIRO; ANO TAKASHI  日本生物工学会大会講演要旨集  67th-  213  -213  2015/09
• ササユリ(Lilium japonicum)香気成分の分析とその抗菌活性

  TAKAHASHI KOKI; MATSUKAWA TETSUYA; TAKIGAWA YOSHIHIRO; ARAI SHIGERU; AKINO TOSHIHARU; KAJIYAMA SHIN'ICHIRO  日本生物工学会大会講演要旨集  67th-  339  -339  2015/09
• 未利用農産資源の機能性素材としての開発研究―マンゴー葉の美白作用―

  伊藤仁久; 志水恒介; 堀川勇次; 友廣教道; 文室政彦; 宇都宮直樹; 飯嶋盛雄; 高田雄輔; 松川哲也; 松川哲也; 梶山慎一郎; 赤井康平; 中垣友太; 村田和也; 松田秀秋; 松田秀秋  日本生薬学会年会講演要旨集  62nd-  102  2015/08
• ウンシュウミカン(Citrus unshiu)葉のリパーゼ阻害活性物質

  TAKATA YUSUKE; MATSUKAWA TETSUYA; ITO KIMIHISA; SHIMIZU KOSUKE; HORIKAWA YUJI; TOMOHIRO NORIMICHI; TSUMOTO TERUYOSHI; HASHIZUME JUNJI; FUJITA TAKUYA; FUMIMURO MASAHIKO; UTSUNOMIYA NAOKI; NAKAGAKI YUTA; MURATA KAZUYA; MATSUDA HIDEAKI; KAJIYAMA SHIN'ICHIRO  日本農芸化学会大会講演要旨集(Web)  2015-  3F37A15 (WEB ONLY)  2015/03
• 2P-155 Isolation of antifungal active substance from actinomycetes

  Uetsuki Yoshihiro; Ohike Tatsuya; Matsukawa Tetsuya; Okanami Masahiro; Kajiyama Shin'ichiro; Ano Takashi  日本生物工学会大会講演要旨集  67-  2015
• 3P-274 Volatile flavor components of Lillium japonicum and their antimicrobial activity

  Takahashi Koki; Matsukawa Tetsuya; Takikawa Yoshihiro; Arai Shigeru; Akino Toshiharu; Kajiyama Shin'ichiro  日本生物工学会大会講演要旨集  67-  339  -339  2015
• カンキツ植物の生活習慣病に対する有効性に関する研究―ウンシュウミカン(Citrus unshiu)葉の抗肥満作用について

  ITO KIMIHISA; SHIMIZU KOSUKE; HORIKAWA YUJI; TOMOHIRO NORIMICHI; TSUMOTO MITSUTAKA; HASHIZUME JUNJI; FUJITA TAKUYA; FUMURO MASAHIKO; UTSUNOMIYA NAOKI; TAKADA YUSUKE; MATSUKAWA TETSUYA; MATSUKAWA TETSUYA; KAJIYAMA SHIN'ICHIRO; NAKAGAKI TOMOTA; MURATA KAZUYA; MATSUDA HIDEAKI; MATSUDA HIDEAKI  日本薬学会年会要旨集(CD-ROM)  135th-  ROMBUNNO.27PB-AM159  2015
• 続・生物工学基礎講座 バイオよもやま話 天然物由来成分に騙されるな(天然物は本当に安全なの?)

  MATSUKAWA TETSUYA; KAJIYAMA SHIN'ICHIRO  生物工学会誌  92-  (10)  556  -559  2014/10
• 3P-101 Evaluation of Trichoderma atroviride HNT-01 as biocontrol agent

  Ohike Tatsuya; Matsukawa Tetsuya; Okanami Masahiro; Kajiyama Shin'ichiro; Yamamoto Shigeki; Hirose Yoichiro; Ano Takashi  日本生物工学会大会講演要旨集  66-  220  -220  2014/08
• 3P-100 Development of biocontrol agents from actinomycetes

  Uetsuki Yoshihiro; Ohike Tatsuya; Matsukawa Tetsuya; Okanami Masahiro; Kajiyama Shin'ichiro; Ano Takashi  日本生物工学会大会講演要旨集  66-  219  -219  2014/08
• 2P-232 Analysis of metabolic changes in volatile compounds in Citrus leaves against environmental stress

  Asai Tomonori; Matsukawa Tetsuya; Kajiyama Shin'ichiro  日本生物工学会大会講演要旨集  66-  164  -164  2014/08
• カンキツ葉中の香気成分の環境応答反応における代謝変動解析

  ASAI TOMONORI; MATSUKAWA TETSUYA; KAJIYAMA SHIN'ICHIRO  日本生物工学会大会講演要旨集  66th-  164  -164  2014/08
• 放線菌を用いた微生物農薬の開発

  UETSUKI YOSHIHIRO; OIKE TATSUYA; MATSUKAWA TETSUYA; OKANAMI MASAHIRO; KAJIYAMA SHIN'ICHIRO; ANO TAKASHI  日本生物工学会大会講演要旨集  66th-  219  -219  2014/08
• SRS顕微法によるジャトロファ種子油分の非破壊解析

  SASAI TOMOHIRO; TSUCHIMOTO SUGURU; SAKAI TOSHIE; WADA NAOKI; OZEKI YASUYUKI; KAJIYAMA SHIN'ICHIRO; ITO KAZUYOSHI; FUKUI KIICHI  日本生物工学会大会講演要旨集  66th-  141  -141  2014/08
• 十和田石粉末から単離したTrichoderma atroviride HNT‐01の微生物農薬としての評価

  OIKE TATSUYA; MATSUKAWA TETSUYA; OKANAMI MASAHIRO; KAJIYAMA SHIN'ICHIRO; YAMAMOTO SHIGEKI; HIROSE YOICHIRO; ANO TAKASHI  日本生物工学会大会講演要旨集  66th-  220  -220  2014/08
• 2P-139 Non-destructive analysis of jatropha seed oil by SRS microscopy

  Sasai Tomohiro; Tsuchimoto Suguru; Sakai Toshie; Wada Naoki; Ozeki Yasuyuki; Kajiyama Shin'ichiro; Ito Kazuyoshi; Fukui Kiichi  日本生物工学会大会講演要旨集  66-  141  -141  2014/08
• Comprehensive analysis of wound induced metabolic change in citrus plants

  Tomonori Asai; Tetsuya Matsukawa; Atsushi Ishihara; Shin'ichiro Kajiyama  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY  248-  2014/08
• Jatropha curcas種子採油残渣を用いた微生物農薬の培養

  石田結子; 大池達矢; 松川哲也; 岡南政宏; 梶山慎一郎; 阿野貴司  日本農芸化学会大会講演要旨集(Web)  2014-  2A14P15 (WEB ONLY)  2014/03
• 低栄養性細菌を用いた微生物農薬候補株の探索

  OIKE TATSUYA; MATSUKAWA TETSUYA; OKANAMI MASAHIRO; KAJIYAMA SHIN'ICHIRO; ANO TAKASHI  日本農芸化学会大会講演要旨集(Web)  2014-  2A14P14 (WEB ONLY)  2014/03
• 日本在来藍のインディカン生産に対するストレスホルモンおよび紫外線の影響

  TAKATA YUSUKE; MATSUKAWA TETSUYA; HORIBATA AKIRA; KAJIYAMA SHIN'ICHIRO  日本農芸化学会大会講演要旨集(Web)  2014-  2C05A13 (WEB ONLY)  2014/03
• ナンヨウアブラギリ由来フォルボールエステル粗画分の発がんプロモーション活性

  正田将大; 玉置恵子; 村上ひかる; 松川哲也; 梶山慎一郎  日本生物工学会大会講演要旨集  65th-  161  -161  2013/08
• 2P-229 Tumor promoting activity of crude phorbol ester fraction from Jatropha curcas

  Shoda Masahiro; Tamaki Keiko; Murakami Hikaru; Matsukawa Tetsuya; Kajiyama Shin'ichiro  日本生物工学会大会講演要旨集  65-  161  -161  2013/08
• 1P-206 Production of antifungal lipopeptide iturin A by glycerol as BDF by-product

  Ohike Tatsuya; Hashigami Kohei; Ishida Yuko; Matsukawa Tetsuya; Okanami Masahiro; Kajiyama Shin'ichiro; Ano Takashi  日本生物工学会大会講演要旨集  65-  69  -69  2013/08
• Jatropha curcasの種子採油残渣を用いた微生物農薬の開発

  吉川諒; 大池達矢; 松川哲也; 岡南政宏; 梶山慎一郎; 阿野貴司  日本農芸化学会大会講演要旨集(Web)  2012-  2012
• ジャトロファフォルボールエステル分解微生物の探索

  大池達矢; 松川哲也; 岡南政宏; 阿野貴司; 梶山慎一郎  日本農芸化学会大会講演要旨集(Web)  2012-  2012
• 2Bp10 Isolation and characterization of wound-induced compounds in Citrus hassaku leaves

  Asai Tomonori; Matsukawa Tetsuya; Ishihara Atsushi; Kajiyama Shin'ichirou  日本生物工学会大会講演要旨集  63-  122  -122  2011/08
• ハッサク葉に含まれる傷害誘導性物質の単離と同定

  浅井智紀; 松川哲也; 石原亨; 梶山慎一郎  日本農芸化学会大会講演要旨集  2011-  2011
• ジャトロファ種子1粒を用いた脂質およびフォルボールエステル類の分析

  大池達矢; 松川哲也; 岡南政宏; 阿野貴司; 梶山慎一郎  日本農芸化学会大会講演要旨集  2010-  2010
• 2Hp02 Quantitative analysis of phorbol esters in the wastes from Jatropha bio-diesel production

  Suzuki Haruka; Matsukawa Tetsuya; Kajiyama Shin'ichiro  日本生物工学会大会講演要旨集  21-  109  -109  2009/08
• 3S12a01 Single cell based analysis of oat difence responces

  KAJIYAMA Shin'ichiro; Izumi Yashihiro; Nakamura Ryosuke; Ishihara Atsushi; Okazawa Atsushi; Fukusaki Eiichiro; Kanematsu Yasuo; Kobayashi Akio  日本生物工学会大会講演要旨集  21-  269  -269  2009/08
• コムギ硬軟質に関わるfriabilin関連遺伝子群のイネへの導入と蓄積

  鈴木剛; 和田幸雄; 和田英雄; 後藤公美; 中野亜紀子; 和田直樹; 梶山慎一郎; 福井希一; 向井康比己  育種学研究  11-  2009
• 2Aa01 Development of dispersion method for hydrophobic bioactive substances in water using laser ablation

  FUKADA Yosuke; KAJIYAMA Shin'ichiro; ASAHI Tsuyoshi; YASUKUNI Ryohei; FUKUI Kiichi  日本生物工学会大会講演要旨集  20-  59  -59  2008/07
• 1Cp14 A novel breeding method for yeast producing a high level of gamma-aminobutyric acid by femtosecond laser.

  TSUTSUMI Hiroko; MIZUMOTO Makiko; INDO Masaharu; SAKAKURA Masaaki; HASHIMOTO Takuya; KAJIYAMA Shinichiro; FUKUSAKI Eiichiro; UEDA Mitsuyoshi; HIRAO Kazuyuki; HATA Yoji  日本生物工学会大会講演要旨集  20-  96  -96  2008/07
• Protection of isolated chromosomes using calcium-alginate microbeads(bioactive beads)toward chemical and physical stress.

  梶山 慎一郎  Chromosome Sci. (印刷中)  2008  [Refereed]
  
• Protection of isolated chromosomes using calcium-alginate microbeads(bioactive beads) toward chemical and physical stress

  梶山 慎一郎  Chromosome Sci (in printing)  2008
• 2Ap04 Development of high speed screening system for enzyme surface displaying yeast by flow sorting :

  ZHOU Yan; KAJIYAMA Shin'ichiro; ITOH Kouichi; TANINO Takanori; FUKUDA Nobuo; KONDO Akihiko; FUKUI Kiichi  日本生物工学会大会講演要旨集  20-  63  -63  2008
• 「細胞一つだけ取り出し」

  梶山 慎一郎  日本経済新聞 関西版 科学欄  2008
• O-26. Entrapment of HeLa cell chromosome in bioactive beads(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :

  MURAKAWA Tomoko; HAYASHIHARA Kayoko; KAJIYAMA Shin'ichiro; FUKUI Kiichi  Chromosome science  10-  (4)  120  -120  2007
• How to use production potential of photosynthetic bacteria and plants for global warning

  小林 昭雄; 梶山 慎一郎; 岡澤 敦司  Lichenology  6-  (2)  154  -157  2007
• 62.Development of a novel quantification system of transcripts using mass spectrometer

  Hirozane Yoshihiko; Fukusaki Eiichiro; Kajiyama Shinichiro; Kobayashi Akio  植物化学調節学会研究発表記録集  41-  (41)  77  -77  2006/10
• 63.High sensitive nano flow LC-ESI-MS analysis using packed nano capillary column integrated with nano-sprayer for plant metabolite anaylysis

  Izumi Yoshihiro; Fukusaki Eiichiro; Kajiyama Shinichiro; Kobayashi Akio  植物化学調節学会研究発表記録集  41-  (41)  78  -78  2006/10
• 2H12-3 Expression analysis of error-prone DNA polymerases in human cultured cells under stress condition

  HIRANO Miki; KURIHARA Sachiko; AKIYAMA Yukio; KAJIYAMA Shin'ichiro; FUKUI Kiichi  日本生物工学会大会講演要旨集  18-  142  -142  2006/08
• 3B09-4 Development of a novel quantification system of transcripts using mass spectrometer

  HIROZANE Yoshihiko; FUKUSAKI Eiichiro; KAJIYAMA Shin'ichiro; KOBAYASHI Akio  日本生物工学会大会講演要旨集  18-  32  -32  2006/08
• 3A-AM5 Laser technology for single cell sampling and analysis

  KAJIYAMA Shin'ichiro; KOBAYASHI Akio; FUKUI Kiichi; ITOH Kazuyoshi  日本生物工学会大会講演要旨集  18-  13  -13  2006/08
• 2H12-1 Analysis of transcripts using ESI-MS(I) : investigation of ionization condition

  KAJIWARA Shin'ichi; RIHARA Yoshiyuki; IZUMI Yoshihiro; HARADA Kazuo; FUKUSAKI Ei-ichiro; KOBAYASHI Akio  日本生物工学会大会講演要旨集  17-  221  -221  2005/09
• 3S2-AM6 Application of plused UV laser micro-processing for plant cells

  KAJIYAMA Shin'ichiro; KOBAYASHI Akio  日本生物工学会大会講演要旨集  16-  42  -42  2004/08
• 紫外線パルスレーザを用いた新しい植物形質転換法の開発(XII)-アグロバクテリウムVirタンパク質を利用した遺伝子導入効率の改善-

  梶山慎一郎; 小川景子; 北野晃浩; 福崎英一郎; 原島俊; 福井希一; 小林昭雄  日本農芸化学会大会講演要旨集  2004-  2004
• 47(P-9) Single cell analysis of plant metabolites using LASER micro sampling

  Kobayashi A; Kajiyama S; Watabe T; Harada K; Fukusaki E  天然有機化合物討論会講演要旨集  45-  (45)  275  -280  2003/09
• Application of UV pulsed laser micro beam to plant cells (IV) : Acquisition of stable transgenic plants by the laser gene introduction

  Inoue Fumihide; Kajiyama Shinichiro; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  15-  119  -119  2003/08
• Application of UV pulsed laser micro beam to plant cells (V) : An approach to huge-sized DNA introduction to plant cells

  Tabata Atsushi; Kajiyama Shinichiro; Fukusaki Eiichiro; Harashima Satoshi; Fukui Kiichi; Kobayashi Akio  日本生物工学会大会講演要旨集  15-  119  -119  2003/08
• Application of UV pulsed laser micro beam to plant cells (VI) : One cell analysis of petal pigments using laser microsampling

  Kajiyama Shinichiro; Watabe Tatsuya; Harada Kazuo; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  15-  119  -119  2003/08
• Analysis of molecular mechanism of autumnal tints : Localization analysis of Ginkgo biloba chlorophyllase

  Okazawa Atsushi; Itoh Yoshiko; Tang Lei; Kajiyama Shinichiro; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  15-  173  -173  2003/08
• Transformation of tobacco SR-1 using bio-active beads

  Murakawa Tomoko; Kawabe Akira; Liu Haibo; Yanagisawa Masanobu; Kajiyama Shinichiro; Kobayashi Akio; Harashima Satoshi; Matsunaga Sachihiro, Kiichi  日本生物工学会大会講演要旨集  15-  184  -184  2003/08
• Expression of Aspergillus niger pectin methylesterase in transgenic tobacco (Nicotiana tabacum) leads to alterations on the level of methanol and a dwarf phenotype

  Hasunuma Tomohisa; Fukusaki Eiichiro; Okazawa Atsushi; Kajiyama Shinichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  15-  184  -184  2003/08
• 47(P-9) Single cell analysis of plant metabolites using LASER micro sampling

  Kobayashi A; Kajiyama S; Watabe T; Harada K; Fukusaki E  Symposium on the Chemistry of Natural Products, symposium papers  45-  (0)  275  -280  2003
• レーザーマイクロサンプリングを用いた植物代謝産物1細胞分析の試み

  梶山 慎一郎; 小林昭雄; 渡部達也; 原田和生; 福崎英一郎  天然有機化合物討論会講演要旨集  45-  275  -280  2003
• 951 Application of UV plused laser microbeam technique to plant cells (III) : Analysis of plant wounding responses to plused UV laser

  Shoji Takeshi; Kajiyama Shinichiro; Kawaoka Akiyoshi; Yoshida Kazuya; Shinmyo Atsuhiko; Yamaguchi Yube; Sano Hiroshi; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  14-  177  -177  2002/09
• 950 Application of UV pulsed laser microbeam technique to plant cells (II) : Sophisticated approach to one cell metabolites analysis

  Kajiyama Shinichiro; Yoshikawa Norihiro; Watabe Tatsuya; Shoji Takeshi; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  14-  177  -177  2002/09
• 949 Application of UV pulsed laser microbeam technique to plant cells (I) : Laser microprocessing and its application

  Yoshikawa Norihiro; Shoji Takeshi; Kajiyama Shinichiro; Fukusaki Eiichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  14-  177  -177  2002/09
• 537 Elucidation of a molecular recognition mechanism of the chitin binding penticles

  Fukusaki Eiichiro; Ogawa Koichi; Kajiyajma Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  14-  82  -82  2002/09
• カプサイシンDHA誘導体(Dohevanil)のHela細胞及びHela-R細胞に対する作用

  高畑京也; 石畑公江; 金永福; 馬場直道; 多田幹郎; 梶山慎一郎; 福崎英一郎; 小林昭雄  脂質栄養学  11-  (2)  2002
• 紫外線パルスレーザを用いた新しい植物形質転換法の開発 (III) 巨大DNAデリバリーを目的とした遺伝子導入用ビーズの開発

  高倉友紀子; 水上温司; 長森英二; 曽根岳史; 梶山慎一郎; 福崎英一郎; 原島俊; 小林昭雄; 福井希一  日本農芸化学会大会講演要旨集  2002-  2002
• 紫外線パルスレーザを用いた新しい植物形質転換法の開発 (IV) DNA含有ビーズ(バイオビーズ)を用いた新規な形質転換法

  長森英二; 水上温司; 高倉友紀子; 曽根岳史; 梶山慎一郎; 福崎英一郎; 原島俊; 小林昭雄; 福井希一  日本農芸化学会大会講演要旨集  2002-  2002
• キチンを認識するオリゴペプチドの分子認識様式の解析

  福崎英一郎; 小川公一; 梶山慎一郎; 岡沢敦司; 小林昭雄  日本生物工学会大会講演要旨集  2002-  2002
• 33 Structure of Hetero Polysaccharide from Desiccation-tolerant Terrestrial Cyanobacterium Nostoc commune

  Kajiyama Shin'ichiro; Yagi Masahiro; Kurihara Tatsuyuki; Okazawa Atsushi; Fukusaki Ei-ichiro; Kobayashi Akio  天然有機化合物討論会講演要旨集  43-  (43)  193  -198  2001/09
• Structure elucidation of a tetramic acid having an antimicrobial activity, produced in callus and fungus co-culture system.

  Marfori E. C; Kajiyama Shin'ichiro; Okazawa Atsushi; Fukusaki Ei-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  13-  165  -165  2001/08
• Analysis of yellowing mechanism of Ginkgo biloba leaves : Cloning and expression of chlorophyllase

  Okazawa Atsushi; Itoh Yoshiko; Tang Lei; Fukusaki Ei-ichiro; Kajiyama Shin-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  13-  185  -185  2001/08
• ウツボカズラ科の食虫植物Nepenthes alataの捕虫袋におけるペプチドの消化吸収過程の解析

  安忠一; 竹川昇志; 岡沢敦司; 福崎英一郎; 梶山慎一郎; 小林昭雄  日本農芸化学会誌  75-  2001
• 33 Structure of Hetero Polysaccharide from Desiccation-tolerant Terrestrial Cyanobacterium Nostoc commune

  Kajiyama Shin'ichiro; Yagi Masahiro; Kurihara Tatsuyuki; Okazawa Atsushi; Fukusaki Ei-ichiro; Kobayashi Akio  Symposium on the Chemistry of Natural Products, symposium papers  43-  (0)  193  -198  2001
• Peptide-degrading capacity of pitcher fluid from the carnivorous plant Nepenthes alata.

  AN Chung-I; FUKUSAKI Ei-ichiro; TAKEKAWA Shoji; OKAZAWA Atsushi; KAJIYAMA Shin-ichiro; KOBAYASHI Akio  Chemical Regulation of Plants  35-  (2)  223  -223  2000/12
• Effects of C1 compounds on plant metabolisms (part7) : Analysis of the proteins responding to methylamine treatment in rice plant

  FUKUSAKI Ei-ichiro; KAWAMOTO Yuri; SHIRAISHI Takehiko; KAJIYAMA Shinichiro; OKAZAWA Atsushi; KOBAYASI Akio  Chemical Regulation of Plants  35-  (2)  226  -226  2000/12
• Effects of C1 compounds on plant metabolisms (part 6) : Analysis of the genes responding to methylamine treatment in rice plant

  SHIRAISHI Takehiko; WATANABE Tetsuya; FUKUSAKI Ei-ichiro; KAJIYAMA Shinichiro; OKAZAWA Atsushi; KOBAYASHI Akio  Chemical Regulation of Plants  35-  (2)  226  -227  2000/12
• Peptide-degrading capacity of pitcher fluid from the carnivorous plant Nepenthes alata.

  An Chung-Il; Fukusaki Ei-ichiro; Takekawa Shoji; Okazawa Atsushi; Kajiyama Shin-ichiro; Kobayashi Akio  植物化学調節学会研究発表記録集  35-  (35)  29  -30  2000/11
• Effects of C1 compounds on plant metabolisms (part 7) : Analysis of the proteins responding to methylamine treatment in rice plant

  Fukusaki Ei-ichiro; Kawamoto Yuri; Shiraishi Takehiko; Kajiyama Shinichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  35-  (35)  45  -46  2000/11
• Effect of C1 compounds on plant metabolisms (part 6) : Analysis of the genes responding to methylamine treatment in rice plant

  Shiraishi Takehiko; Watanabe Tetsuya; Fukusaki Ei-ichiro; Kajiyama Shinichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  35-  (35)  47  -48  2000/11
• The analysis of viscous colony where Escherichia coli and Dictyostelium discoidium are coexisting.

  Oki S; Todoriki M; Kajiyama S; Fukuzaki E; Kobayash A; Urabe I; Yomo T  Biophysics  40-  (1)  S98  2000/08
• In vitro selection of DNA aptamers that bind specifically to tubulin

  Hasunuma Tomohisa; Fukusaki Ei-ichiro; Okazawa Atsushi; Kajiyama Shin-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  12-  148  -148  2000/07
• Structure elucidation of exocellular polysaccharide produced by desiccation tolerant cyanobacterium Nostoc sp.

  Kajiyama Shin'ichiro; Yagi Masahiro; Fukusaki Eiichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  12-  236  -236  2000/07
• DNA aptamers that bind to chitin

  E Fukusaki; T Kato; H Maeda; N Kawazoe; Y Ito; A Okazawa; S Kajiyama; A Kobayashi  BIOORGANIC & MEDICINAL CHEMISTRY LETTERS  10-  (5)  423  -425  2000/03
• 949 The Properties of Cyanobacterial Heteropolysaccharide Degrading Enzyme from a Soil Bacterium :

  Kajiyama Shin'ichiro; Martinis Dasman; Fukusaki Ei-ichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  12-  229  -229  2000
• An artificial plastic receptor that discriminates axial asymmetry.(共著)

  EI-ICHIRO FUKUSAKI; AKIRA SAIGO; SHIN-ICHIRO KAJIYAMA; AKIO KOBAYASHI  ┣DBJ. Biosci. Bioeng.(/)-┫DB  90-  (6)  665  -668  2000
• Effects of C1 compounds on plant metabolisms (part4) : protecting effects against photoinhibition.

  SHIRAISHI Takehiko; FUKUSAKI Ei-ichiro; MIYAKE Chikahiro; YOKOTA Akiho; KAJIYAMA Shin-ichiro; OKAZAWA Atsushi; KOBAYASHI Akio  Chemical Regulation of Plants  34-  (2)  351  -352  1999/12
• Effects of C1 compounds on plant metabolisms (part5) : C1 compounds metabolism in Arabidopsis thaliana.

  FUKUSAKI Ei-ichiro; IKEDA Tatsuhiko; NISHIKAWA Takashi; SHIRAISHI Takehiko; KAJIYAMA Shin-ichiro; OKAZAWA Atsushi; KOBAYASHI Akio  Chemical Regulation of Plants  34-  (2)  352  -352  1999/12
• Analyses of the expression pattern of the genes stimulated by elicitor in French bean plant.

  FUKUSAKI Ei-ichiro; MATSUNAWA Ayako; WATANABE Tetsuya; KAJIYAMA Shin-ichiro; OKAZAWA Atsushi; KOBAYASHI Akio  Chemical Regulation of Plants  34-  (2)  359  -359  1999/12
• cDNA cloning of plasma membrane proton pump of carnivorous plant Nepenthes hybrida.

  FUKUSAKI Ei-ichiro; AN Tadaichi; KAJIMYAMA Shin-ichiro; OKAZAWA Atsushi; KOBAYASHI Akio  Chemical Regulation of Plants  34-  (2)  359  -359  1999/12
• 61 Effects of C1 compounds on plant metabolisms (part4)(protecting effects against photoinhibition)

  Shiraishi Takehiko; Fukusaki Ei-ichiro; Miyake Chikahiro; Yokota Akiho; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  34-  (34)  121  -122  1999/10
• 62 Effects of C1 compounds on plant metabolisms (part5)(C1 compounds metabolism in Arabidopsis thaliana)

  Fukuzaki Ei-ichiro; Ikeda Tatsuhiko; Nishikawa Takashi; Shiraishi Takehiko; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  34-  (34)  123  -124  1999/10
• 85 Analyses of the expression pattern of the genes stimulated by elicitor in French bean plant

  Fukusaki Ei-ichiro; Matsunawa Ayako; Watanabe Tetsuya; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  34-  (34)  163  -164  1999/10
• 86 cDNA cloning of plasma membrane proton pump of carnivorous plant Nepenthes hybrida

  Fukusaki Ei-ichiro; An Tadaichi; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  34-  (34)  165  -166  1999/10
• A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry

  T Shiraishi; E Fukusaki; S Kajiyama; A Kobayashi  JOURNAL OF CHROMATOGRAPHY A  855-  (1)  337  -340  1999/09
• Structure elucidation of exocellular polysaccharide produced by desiccation tolerant terrestrial cyanobacterium Nostoc sp.

  Kajiyama Shin'ichiro; Yagi Masahiro; Fukusaki Eiichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  11-  63  -63  1999/08
• Chlorophyll catabolism in yellowing of Ginkgo green leaves

  Okazawa Atsushi; Tang Lei; Fukusaki Ei-ichiro; Kajiyama Shin-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  11-  64  -64  1999/08
• In vitro selection of DNA aptamers that bind specifically to hematoporphyrin

  Okazawa Atsushi; Maeda Hiroshi; Fukusaki Ei-ichiro; Kajiyama Shin-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  11-  64  -64  1999/08
• 421 ISOLATION AND CHARACTERIZATION OF BIO-ACTIVE COMPOUNDS PRODUCED BY THE CO-CULTURE OF A CALLUS AND A FUNGUS :

  Kajiyama Shin'ichiro; Marfoni Eufrocinio; Fukusaki Eiichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  11-  63  -63  1999
• A-10 Effect of C1 compounds on the plant metabolism : Growth stimulation by formate in rice plant

  FUKUZAKI Eiichiro; SHIRAISHI Takehiko; KAJIYAMA Shin-ichiro; OKAZAWA Atsushi; KOBAYASHI Akio  植物化学調節学会研究発表記録集  33-  (33)  29  -30  1998/10
• Radical coupling reaction of hydroxystilbene by Vitacea cell culture.

  Fukusaki Eiichiro; Kobayashi Yoshitaka; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  日本生物工学会大会講演要旨集  10-  102  -102  1998/08
• 76(P55) Microbial flora controlling substances in the jelly clumps of Nostoc commune

  Kajiyama Shin-ichiro; Nakao Motonao; Kobayashi Akio; Sasaki Katsuharu; Kawazu Kazuyoshi  天然有機化合物討論会講演要旨集  (40)  449  -454  1998/08
• 76(P55) Microbial flora controlling substances in the jelly clumps of Nostoc commune

  Kajiyama Shin-ichiro; Nakao Motonao; Kobayashi Akio; Sasaki Katsuharu; Kawazu Kazuyoshi  Symposium on the Chemistry of Natural Products, symposium papers  40-  (0)  449  -454  1998
• 21 Gene expression of phenylpropanoid biosynthetic enzyme in elicitor treated French bean

  Matsunawa Ayako; Watanabe Takashi; Fukusaki Ei-ichiro; Kajiyama Shin-ichiro; Okazawa Atsushi; Kobayashi Akio  植物化学調節学会研究発表記録集  32-  (32)  47  -48  1997/11
• Isolation and analysis of genes induced by elicitor

  Fukusaki Ei-ichirou; Watanabe Takashi; Kajiyama Sin-ichirou; Kobayashi Akio  日本生物工学会大会講演要旨集  9-  146  -146  1997/09
• 58 イネ科植物における有機化合物の選択的取込み

  小林 昭雄; 高畠 陽平; 河津 一儀; 梶山 慎一郎; 福崎 英一郎  植物化学調節学会研究発表記録集  31-  (31)  115  -116  1996/10
• Elicitor activities of chimera oligosaccharides consisting of glucose and glucosamine.

  Fukusaki Eiichiro; Yabushita Setsuko; Nakanishi Tsuyoshi; Kajiyama Shin-ichiro; Kobayashi Akio  日本生物工学会大会講演要旨集  8-  286  -286  1996/09
• SECONDARY METABOLITE PRODUCTION BY Tectona grandis CELL SYSTEM

  Marwani Erly; Kajiyama Shin-Ichiro; Fukusaki Eiichiro; Kawazu Kazuyoshi; Kobayashi Akio  Journal of the Agricultural Chemical Society of Japan  70-  84  -84  1996/03
• ペルオキシダーゼによって誘導される強力な細胞毒成分の同定と酵素合成 : 有機化学・天然物化学

  小林 昭雄; 馬場 健史; 佐々木 克晴; 萩原 弘達; 梶山 慎一郎; 神崎 浩; 河津 一儀  日本農藝化學會誌  70-  (0)  52  -52  1996/03
• ラン藻Nostoc communeの産生する有糸分裂阻害物質(第三報) : 有機化学・天然物化学

  梶山 慎一郎; 佐々木 克晴; 山崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  70-  (0)  52  -52  1996/03
• ペルオキシダーゼ反応により誘導される生理活性物質(1) : 有機化学・天然物化学

  小林 昭雄; 馬場 健史; 梶山 慎一郎; 神崎 浩; 河津 一儀  日本農藝化學會誌  70-  (0)  58  -58  1996/03
• ペルオキシダーゼ反応によって誘導される生理活性物質(2) : 有機化学・天然物化学

  福崎 英一郎; 大上 忠宏; 梶山 慎一郎; 小林 昭雄  日本農藝化學會誌  70-  (0)  59  -59  1996/03
• ラン藻Nostoc communeの純粋化と培養条件による代謝産物の変化 : 生物化学工学

  梶山 慎一郎; 野村 健三; 神崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  70-  (0)  129  -129  1996/03
• Production of bioactive compounds by peroxidase reaction system

  Kobayashi Akio; Bamba Takeshi; Kajiyama Shin-ichiro; Kawazu Kazuyoshi  日本生物工学会大会講演要旨集  7-  264  -264  1995/09
• Isolation of a Cytotoxic Compound, MI-1 from Tamarindus indica and the Structure-Activity Relationship Studies of Its Analogs

  Adenan M.I.; Kajiyama S.; Kanzaki H.; Kawazu K.; Kobayashi A.  Journal of the Agricultural Chemical Society of Japan  69-  46  -46  1995/07
• ラン藻Nostoc sp.の産生する有糸分裂阻害物質(第二報) : 有機化学・天然物化学

  梶山 慎一郎; 佐々木 克晴; 神崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  69-  (0)  46  -46  1995/07
• ラミナラン由来エリシターのインゲンに対する活性 : 糖鎖工学

  田井 章博; 大澤 恵理; 梶山 慎一郎; 神崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  69-  (0)  99  -99  1995/07
• ムギによるC_6-C_3化合物の生物変換と生成物の抗菌活性及び根圏微生物に対する影響 : 有機化学・天然物化学

  金 明照; 井川 亜希子; 梶山 慎一郎; 神崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  69-  (0)  218  -218  1995/07
• ペルオキシダーゼ反応により得られるグアイアコール重合物の構造と生理活性 : 有機化学・天然物化学

  小林 昭雄; 平木 雅弘; 馬場 健史; 神崎 浩; 梶山 慎一郎; 河津 一儀  日本農藝化學會誌  69-  (0)  223  -223  1995/07
• 陸生ラン藻Nostoc sp.の産生する抗菌活性物質(第二報) : 有機化学・天然物化学

  梶山 慎一郎; 佐々木 克晴; 神崎 浩; 河津 一儀; 小林 昭雄  日本農藝化學會誌  69-  (0)  223  -223  1995/07
• Streptomyces sp.923103株の生産するマツノザイセンチュウ増殖阻害物質 : 有機化学・天然物化学

  河津 一儀; 柏 秀樹; 神崎 浩; 梶山 慎一郎; 小林 昭雄  日本農藝化學會誌  69-  (0)  243  -243  1995/07
• Streptomyces sp.A1212株が生産する昆虫トレハラーゼ阻害物質 : 有機化学・天然物化学

  神崎 浩; 池田 博幸; 大西 茂彦; 梶山 慎一郎; 小林 昭雄; 河津 一儀  日本農藝化學會誌  69-  (0)  243  -243  1995/07
• A3-17 アルファルファにたいしてエリシター活性を示すラミナラン由来オリゴ糖の構造

  田井 章博; 梶山 慎一郎; 神崎 浩; 河津 一儀; 小林 昭雄  植物化学調節学会研究発表記録集  (29)  43  -44  1994/10
• A3-18 リゾチタームの触媒する糖転移反応により合成したキトオリゴ糖の植物エリシター活性

  秋山 康紀; 梶山 慎一郎; 神崎 浩; 河津 一儀; 小林 昭雄  植物化学調節学会研究発表記録集  (29)  45  -46  1994/10
• Enzymatic synthesis of elicitor-active thiophenyl hetero-N-acetyl-chitooligosaccharides utilizing lysozyme-catalyzed transglycosylation reaction.

  Kobayashi Akio; Kanzaki Hiroshi; Akiyama Kohki; Kajiyama Shin-ichiro; Kawazu Kazuyoshi  日本生物工学会大会講演要旨集  6-  209  -209  1994/10
• 41 植物防御系においてペルオキシターゼが関与する化学的防御機構の解析

  小林 昭雄; 小口 泰; 梶山 慎一郎; 神崎 浩; 河津 一義  植物化学調節学会研究発表記録集  (28)  75  -76  1993/10
• 44 病原真菌細胞壁エリシターとモデルオリゴ糖エリシターのエンドウに対するファイトアレキシン及び類縁化合物の誘導能の比較

  秋山 康紀; 入佐 英紀; 梶山 慎一郎; 神崎 浩; 河津 一義; 小林 昭雄  植物化学調節学会研究発表記録集  (28)  81  -82  1993/10
• 47 THE ROLE OF PEROXIDASE IN THE CHEMICAL DEFENCE OF PLANTS : A NOVEL PEROXIDASE INHIBITOR AND A NEW TYPE OF BIOACTIVE PHENOLICS PRODUCED BY PEROXIDASE

  Kobayashi A; Koguchi Y; Kanzaki H; Kajiyama S; Kawazu K  天然有機化合物討論会講演要旨集  (35)  361  -368  1993/09
• 細胞骨格作用物質 (細胞機能研究のための低分子プロ-ブ) -- (細胞機能調節物質)

  小林 昭雄; 梶山 慎一郎  蛋白質核酸酵素  38-  (11)  p1730  -1741  1993/08
• Molecular Basis of Probes for Cytoskeletal Proteins.

  小林昭雄; 梶山慎一郎  蛋白質 核酸 酵素  38-  (11)  1993
• 47 THE ROLE OF PEROXIDASE IN THE CHEMICAL DEFENCE OF PLANTS : A NOVEL PEROXIDASE INHIBITOR AND A NEW TYPE OF BIOACTIVE PHENOLICS PRODUCED BY PEROXIDASE

  Kobayashi A; Koguchi Y; Kanzaki H; Kajiyama S; Kawazu K  Symposium on the Chemistry of Natural Products, symposium papers  35-  (0)  361  -368  1993
• 43 SYNTHESIS OF NEW INDOLACTAM ANALOGUES BY MICROBIAL CONVERSION AND THEIR IN VITRO BIOLOGICAL ACTIVITIES RELATED TO TUMOR PROMOTION

  Irie K; Kajiyama S; Okuno S; Koshimizu K; Nishino H; Iwashima A; Hayashi H; Arai M  天然有機化合物討論会講演要旨集  (33)  329  -336  1991/09
• 43 SYNTHESIS OF NEW INDOLACTAM ANALOGUES BY MICROBIAL CONVERSION AND THEIR IN VITRO BIOLOGICAL ACTIVITIES RELATED TO TUMOR PROMOTION

  Irie K; Kajiyama S; Okuno S; Koshimizu K; Nishino H; Iwashima A; Hayashi H; Arai M  Symposium on the Chemistry of Natural Products, symposium papers  33-  (0)  329  -336  1991
• 41 STUDIES ON THE BIOSYNTHESIS OF INDOLE ALKALOID TUMOR PROMOTER TELEOCIDIN

  Irie K; Kajiyama S; Funaki A; Koshimizu K; Hayashi H; Arai M  天然有機化合物討論会講演要旨集  (31)  308  -315  1989/09
• 41 STUDIES ON THE BIOSYNTHESIS OF INDOLE ALKALOID TUMOR PROMOTER TELEOCIDIN

  Irie K; Kajiyama S; Funaki A; Koshimizu K; Hayashi H; Arai M  Symposium on the Chemistry of Natural Products, symposium papers  31-  (0)  308  -315  1989

Industrial Property Rights

• 特許第5501360号:光学顕微鏡およびその制御方法  2014/03/20

  小関 泰之, 伊東 一良, 嶽 文宏, 福井 希一, 梶山 慎一郎, 北川 雄真, 西澤 典彦, 住村 和彦  キヤノン株式会社  201403082232170591
• WO2010-140614:光学顕微鏡、および光学計測  2010/12/09

  小関 泰之, 伊東 一良, 嶽 文宏, 福井 希一, 梶山 慎一郎, 北川 雄真, 西澤 典彦, 住村 和彦  キヤノン株式会社  201203068955148384
• 特開2008-154498:PCRにより増幅させた伸長DNA鎖の検出方法、および、標的配列の測定方法  2008/07/10

  梶山 慎一郎, 福崎 英一郎, 広実 慶彦, 梨原 善行  財団法人大阪産業振興機構, 国立大学法人大阪大学  200903030525076719
• 特開2008-154497:形質転換体の製造方法及び酵母の新規変異株  2008/07/10

  植田 充美, 平尾 一之, 梶山 慎一郎, 福崎 英一郎, 司 金海, 秦 洋二, 堤 浩子  独立行政法人科学技術振興機構, 国立大学法人京都大学, 国立大学法人大阪大学, 月桂冠株式会社  200903065814408841
• 特開2008-114059:レーザ加工装置及びレーザ加工方法  2008/05/22

  植田 充美, 平尾 一之, 梶山 慎一郎, 坂倉 政明, ドゥミトリ トゥジリン, ザポロシェンコ ユーリ, 島田 竜太郎  独立行政法人科学技術振興機構, 国立大学法人京都大学, 国立大学法人大阪大学, 株式会社東京インスツルメンツ  200903059931293400
• 特許第3780334号:細胞の加工方法  2006/03/17

  小林 昭雄, 福井 希一, 原島 俊, 福崎 英一郎, 梶山 慎一郎, 奥田 伸哉, 庄司 猛  国立大学法人大阪大学  201103083781739975  

  特開2002－281970
• 特開2005-080570:生物学的物質の導入法及び当該生物学的物質の導入法を用いた形質転換法生物学的物質の導入方法  2005/03/31

  小林 昭雄, 福井 希一, 原島 俊, 福崎 英一郎, 梶山 慎一郎  大阪大学長  200903076939306732
• 特開2002-325572:外来物質の導入方法  2002/11/12

  小林 昭雄, 福井 希一, 原島 俊, 福崎 英一郎, 梶山 慎一郎, 奥田 伸哉, 庄司 猛  大阪大学長  200903063271881310  

  特開2002－325572
• 特開2002-281970:細胞の加工方法  2002/10/02

  小林 昭雄, 福井 希一, 原島 俊, 福崎 英一郎, 梶山 慎一郎, 奥田 伸哉, 庄司 猛  大阪大学長  200903046054542394
• 特開平9-176090:抗菌・抗カビ・抗炎症活性剤および活性化合物  1997/07/08

  小林 昭雄, 梶山 慎一郎, 神崎 浩, 河津 一儀, モハド・イルハム・アデナン  株式会社紀文食品  200903085318717126
• 特許出願平7-342930「抗菌・抗カビ・抗炎症活性剤および活性化合物」

  *
• 特許出願平7-342924「抗菌・抗カビ・抗炎症活性物質とその製造方法」

  *
• 特許出願平7-342914「抗菌・抗カビ・抗炎症活性物質とその製造方法」

  *
• 特許出願平6-34483「ラン藻由来の抗カビ活性剤およびその製造方法」

  *
• 特許出願平5-223415「抗菌および抗カビ活性物質の製造方法」

  *

Awards & Honors

• 2003 日本生物工学会 論文賞 （共著）
   JPN
• 平成20年 バイオビジネスコンペジャパン 優秀賞
  

Research Grants & Projects

• Development of anti-tumor-promoting reagent based on the safety assessment of fuel crop Jatropha curcas

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2014 -2017 

  Author : KAJIYAMA Shinichiro

   

  Jatropha curcas is a tropical shrub which contains more than 50 %(w/w) liquid neutral lipid suitable for biodiesel production in its seeds and is expected as a raw crop for next generation biofuels. On the other hand, this plant produces phorbol esters having tumor promoting activity as a secondary metabolite other than lipids. In the process of evaluating the safety of this plant, we found that this plant also contains metabolites that act as the counteractant of PMA, which is known as a potent tumor promoter. In this study, we gained insights on the chemical properties and physiological activities of this anti-tumor promoting metabolite of this this plant.
• Preparation of nanoparticle colloids of bioactive substances by laser ablation in water

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2010 -2012 

  Author : ASAHI Tsuyoshi; KAJIYAMA Shin‐ichiro

   

  Intense pulse-laser Irradiation to suspended organic microcrystalline powder in water leads to its fragmentation into nanoparticles. This technique allows fabrication of pure organic nanoparticle colloids without any chemical additives. Here, we applied this technique to water-insoluble flavonoids which are a class of plant secondary metabolites, and succeeded in preparing stable nanoparticle colloids of flavones and isoflavones. We have demonstrated the toxicity assay for HeLa cells, and have discussed potential applications of the laser ablation method to new phamatical agents and drugs.
• Development of novel screening system for enzyme-displaying yeasts using calcium-alginate based beads.

  Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))

  Date (from‐to) : 2009 -2011 

  Author : Shinichiro KAJIYAMA; Tsutomu TANAKA

   

  In this study, a novel screening method for enzyme-displaying yeasts were investigated. Enzyme-displayed yeast cells were captured in the micro-sized calcium alginate beads each by each using newly developed reverse micelle method to prevent diffusion of hydrolyzed fluorescent substrates. Adopting flow sorting to these captured cells, active cells were successfully enriched. This system would be a useful method for high speed screening of yeast cells which display various hydrolyzing enzymes with high activity.
• Nonlinear-optical microscopy for visualizing chiral structures in biological molecules

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2008 -2010 

  Author : ITO Kazuyoshi; OZEKI Yasuyuki; KAJIYAMA Shinichiro

   

  We have developed new optical microscopic imaging techniques which exploit the electronic and vibrational resonances in nonlinear-optical processes. Specifically, we demonstrated (a) the visualization of two-photon absorption resonances in red blood cells and plant petal cells based on stimulated parametric emission microscopy, and (b) background-free imaging of molecular vibrations based on stimulated Raman scattering microscopy.
• Subcellular dissection and dynamic analysis of intracellular metabolites using non-linear photonics

  Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(特定領域研究)

  Date (from‐to) : 2006 -2008 

  Author : Shinichiro KAJIYAMA; Itaru YANAGIHARA

   

  本研究課題では,極短パルスレーザーによる非線形光学効果を利用して,新しい細胞内代謝産物の取得方法と解析方法を開発するとともに,これらを用いて細胞毒性を有する細菌由来アミロイド様凝集性タンパク質の細胞内での作用機作の解析を行った.この過程で,フェムト秒レーザーを用いた細胞微細加工装置により細胞内に凝集するタンパク質部分を切削・回収することに成功するとともに,非線形光学顕微鏡による無線色での細胞の観察に成功した.
• 非食用油糧生産植物由来毒性代謝産物の分析と制御

  Date (from‐to) : 2008
• Investigation on plant intercellular chemical network using laser micro sampling technique

  Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))

  Date (from‐to) : 2006 -2007 

  Author : Shin'ichiro KAJIYAMA

   

  Unlike animal organs controlled by the central nervous system, multicellular plants axe composed of highly independent cells in functional and in metabolic aspect. This means metabolite analysis of whole plant or tissue samples only gives the average information of individual cell functions. For example, upon the infection of pathogens, some plants induce antimicrobial metabolite, phytoalexin. However the way and degree of defense response might not be same among individual cells. In this study, cellular metabolite accumulation and transcription of biosynthetic genes, among individual plant cells are investigated by using the technique of angle cell sampling and the analysis technique i.e. laser assisted single cell sampling, laser micro spectroscopy, and Nano Flow LC-ESI-MS. Through this study, we developed a novel experimental approach for functional cell biology of plants.
• 極短パルスレーザーを用いた細胞内局在性タンパク質作用因子の取得とその網羅的解析

  文部科学省：科学研究費補助金(特定領域研究)

  Date (from‐to) : 2006 -2006 

  Author : 梶山 慎一郎; 柳原 格

   

  近年、細胞間あるいは、細胞内情報伝達やこれに関わる各種生理作用物質の研究が、モレキュラーレベルで論じられるようになってきた。これらの研究は主に、組織や個体を用いたミュータントのフェノタイプの観察あるいは、関連分子のバルク調製による生化学的相互作用の解析に基づいたものがほとんどであるが、より正確で、直接的な知見を得るためには、バルクでの観測ではなく、個々の細胞で実際に起こっている情報伝達をオンサイトで観測し、そこで作用している物質を直接同定することが望ましいことは、言うまでもない。本研究では、細胞毒性を有する細菌由来アミロイド様凝集性タンパク質の細胞内での作用機作の解明、および、常染色体劣性遺伝の疾患の一種、ロバーツ症候群の原因核局在性遺伝子産物の細胞内での作用機作の解明を題材に取り上げ、細胞微細加工技術および、超微量生体物質分析技術を用いて、これらの分子のカウンターパートとなる、ホスト細胞由来相互作用因子の直接同定を試み、当該分野に新知見をもたらすと共に、1細胞を基盤としたライフサーベイヤ研究の重要性を指し示すことを目的としている。H18年度は、(1)極短パルスレーザーを用いたサブセルラーサンプリング装置のセットアップ、(2)GFP融合アミロイド蛋白質毒素TDH(腸炎ビブリオ由来)発現細胞の調製、(3)GFP融合ESCO2蛋白質発現細胞の調製、(4)サブセルラーマイクロサンプリングによる相互作用する蛋白質の取得を検討し、フェムト秒レーザーを用いたサブセラーミクロディセクションによって、GFP標識した細胞毒性を有する細菌由来アミロイド様凝集性タンパク質を切り出すことに成功した。また、微量分析技術として、PCRとLC-MSを組み合わせた新しい転写産物の分析法を確立した。
• シングルセル代謝分析に基づいた植物感染応答反応の動的解析

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2005 -2006 

  Author : 小林 昭雄; 梶山 慎一郎

   

  従来の植物の感染応答研究では主に、個体全体や、組織レベルでの代謝変動や遺伝子発現パターンの追跡に終始しており、個々の細胞で生じている応答のいわば"静的な平均"しか見てこなかった。しかしながら、感染応答メカニズムの全容を捉えるには、細胞の機能分化、すなわち、特定細胞内あるは、細胞間における代謝産物の動態を把握することが重要である。これをなしうるには、試料が生きた状態あるいは、より生に近い状態で個々の細胞の代謝分析を行う技術が必要になる。申請者はこれまで、パルスレーザーを用い、植物組織中の単一細胞に人為的感染反応を生じさせる技術、特定細胞から細胞内容物を取得する技術等の開発に成功している。本研究では、これらの技術と、本特定領域の主眼である顕微分光分析技術さらには近年発展の著しいデータマイニングの手法を組み合わせて、病原微生物感染時に誘起される動的代謝変動を個々の細胞レベルで解析することを目的としている。本年度は、非侵襲で,細胞応答・代謝変動を1細胞レベルで解析するために,レーザー顕微分光解析の検討を行った.エンバク葉肉細胞組織に対してエリシター処理(〜48時間)し,解析を行った結果,アベナンスラミド類に特徴的蛍光を観測し,処理の有無によって蛍光波長-空間マップに明瞭な差異が見られた.また,Capillary LC-ESI-MS分析を用いて解析を行った結果,蛍光波長-空間マップにおける差異が代謝産物の量比と相関関係があることが示された.このことにより、これらの技術・解析手法を用いることでエリシター刺激を受けた細胞及び周辺細胞の時間的・空間的な動的感染応答反応を1細胞レベルで解析可能であることが示唆された.
• Tracking of petal cell maturing process by single-cell based metabolic analysis

  Ministry of Education, Culture, Sports, Science and Technology：Grants-in-Aid for Scientific Research(基盤研究(C))

  Date (from‐to) : 2004 -2005 

  Author : Shin'ichiro KAJIYAMA

   

  In this study, the petal cell maturing process of Torenia plant was investigated by using laser single-cell sampling and analysis technique. In the first year, sample preparation method and laser irradiation condition, and nano-flow LC-MS analysis condition of single-cell origin samples were investigated. Through this investigation, the facile analytical method for petal cell pigment was established. In second year, the quantitative analysis of mRNA concerning petal pigment biosynthesis was investigated. In particular, novel high sensitive quantitative mRNA analysis based on the ESI-MS was developed. In this method, the reporter molecule such as FAM (fluorescein phosphoramidite) was attached to the 5' end of the PCR primer and according to the PCR polymerizing reaction, the reporter molecule and the 5'end nucleotide was released. By quantify the released molecule using ESI-MS, the amount of targeted mRNA in the sample was estimated. The FAM attached nucleotide (FAM-A, FAM-T, FAM-G, FAM-C) was completely separated and quantified by LC-ESI-MS. Using this method, we are succeeded in the estimation of CHS and GADPH quantities in single petal cell of Torenia plant.
• 新規細胞保護剤を指向したネンジュモ属ラン藻細胞外多糖質の解析

  文部科学省：科学研究費補助金(奨励研究(A))

  Date (from‐to) : 1999 -2000 

  Author : 梶山 慎一郎

   

  本研究は、極めて高い細胞外多糖質生産能及び、乾燥耐性を有する陸生ラン藻(Nostoc sp.)を題材とし、細胞外多糖質の精密な構造解析を行い、多糖構造と耐乾燥性との関わりについて知見を得るとともに、新規細胞保護剤開発のための基礎的知見を得ることを目的としている。昨年度の研究により、本多糖は構成糖としてを含むヘテロ多糖であることが判ったため、本年は多糖の部分分解を試み、構造解析を行った.方法としては、(1)0.2NTFA,90℃,5h部分加水分解、(2)本多糖を唯一の炭素源とし、生育可能なバクテリア(Paenibacillus sp.,DS-1)から調製した粗酵素液による分解を試みた。得られたオリゴ糖フラグメントをピリジルアミノ(PA)化し、逆相分取HPLCによりPA化フラグメントを単離した。これら単離したフラグメントについて各種NMR分析を行った。それぞれ^1H-^1H COSYにより各構成糖の1位のプロトンから順次つながりを追って各構成糖を帰属し、さらに、HMQCにより各々のカーボンの帰属を行った後、各構成糖の結合様式をHMBCにより決定した。その結果、(1)の条件からはβ-GlcA-(1→6)-β-Glc-(1→4)-Gal (residue A,MW518)、β-Glc-(1→4)-β-Xyl-(1→4)-[β-GlcA-(1→6)]-β-Glc-(1→4)-Gal(residue B,MW812)が、(2)の条件からはβ-GlcA-(1→6)-β-Glc-(1→4)-α-Gal-(1→4)-β-Glc-(1→4)-Xyl (residue C,MW812)が得られたことが明らかとなった。これらの部分構造より本多糖は、主鎖として→4)-β-Glc-(1→4)-β-Xyl-(1→4)-[β-GlcA-(1→6)]-β-Glc-(1→4)-α-Gal-(1→の繰り返し構造をもち、部分的にD-Man,L-Rhaおよび、未同定の単糖で置換された構造を有しているものと考えられた。
• 食虫植物の有機化合物代謝機構の解析とその応用

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 1997 -1998 

  Author : 小林 昭雄; 藤山 和仁; 梶山 慎一郎; 福崎 英一郎

   

  本研究では、食虫植物の栄養獲得機構に関する予備的な知見を得るために、モデル植物としてウツボカズラ科の食虫植物を用い、捕虫袋内部に分泌される消化酵素や分泌液に関する基礎データの収集を行った。まず、捕虫袋分泌液に含まれる消化酵素の種類を明らかにすべく、各種加水分解酵素の活性測定を行った。予備実験において、蓋(ふた)が開く前の未成熟捕虫袋分泌液は無菌状態であった。一方、昆虫が捕らえられた捕虫袋分泌液は、外見上透明であり、腐敗臭は全く認められないが、数種類の微生物の存在が確認された。その中にはプロテアーゼを分泌するものも存在することが判明した。そこで、植物由来の酵素のみを分析するために、ほぼ無菌的な蓋が開いた直後の捕虫袋分泌液を酵素活性測定に使用した。その結果、プロテアーゼ、エステラーゼ、ホスファターゼ、RNase.DNase、ホスホリパーゼD、キチナーゼといった、少なくとも7種類の酵素活性が存在することが明らかになった。このうちプロテアーゼに関しては、プロテアーゼインヒビターを用いた阻害実験がら、アスパラギン酸プロテアーゼ(酸性プロテアーゼ)である事が判明した。一方で、捕虫袋分泌液のpHは蓋が開いた直後で4.0 4.8、獲物が捕らえられると3以下にまで低下することが予備実験で観察されていた。そこで、分泌液が強酸性を示す原因を探るために、分泌液に含まれる各種無機イオン濃度を測定した。その結果、プロトンの他にK^+とCl^-が高濃度(それぞれ、650 760ppm、530 600ppm)含まれることが明らかとなった。このことから、分泌液の酸性pHは、哺乳類の胃の内部と同じく塩酸の分泌によるものと推測された。以上の結果から、捕虫袋分泌液には昆蚤の消化に必要な消化酵素が一揃い存在し、これら消化酵素と分泌液のpHの低下が本植物の栄養獲得過程において重要な役割を果たしていることが示唆された。今後のさらなる研究により、根以外の部位からの低分子の吸収機構が明らがとなり、葉面がらの効率的物質吸収に関する新しい知識が集積されることが期待される。
• Development of new antimicrobial pressure sensitive adhesive polymer for surgical tape

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 1996 -1998 

  Author : KOBAYASHI Akio; SENDA Shuji; KAJIYAMA Shin'ichiro; FUKUSAKI Eiichiro

   

  At first, we paid an attention to high radical oxidizing activity of peroxidase (POD) from plant to synthesize various antimicrobial compounds. We succeeded to prepare several antimicrobial compounds derived from several phenolics such as guaicol, isoeugenol, ferulic acid, caffeic acid, thymol, carvacrol and hydroxystilben via POD coupling reaction. Among them, we chose a POD reaction product derived from isoeugenol as an antimicrobial compound for further experiments. Next, we prepare several prototypes of adhesive polymer for surgical tapes. We used following polymers such as HPAO, PANTA, PAOB, and NITTO for base adhesive polymer. POD reaction products were mixed above base polymer in toluene to be yielded dope mixture for coating onto the polymer supports. After coating, the preparations were dried up by the drying oven to remove solvent. We examined effects of drug concentrations on their antimicrobial activities and clarified that more than 10% of drug content is essential for expression of antimicrobial activity. Actually, adhesive polymer containing 20% of active drug from isoeugenol show an apparent antimicrobial activity against Staphyrococuss aureus. We suggested that hydrophobicity of isoeugenol POD products cause its low permeability to medium and studied effects of surfactant addition into adhesive polymer. However, addition of surfactant such as Tween 20 or Tween 8O caused aggregation of polymer. We guess that above phenomena was caused from reaction between nonionic surfactant and aromatic multivalent isocyanic crosslinker. In conclusion, PAOB and PANTA were good candidates as base polymers for this study. Further investigation about crosslinker should be performed.
• Diagnosis of plant disease by analysis of peptides and oligosaccharides in the sap.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 1996 -1998 

  Author : KOBAYASHI Akio; KAJIYAMA Shin'ichiro; FUKUSAKI Eiichiro

   

  It is well known that plants use inorganic elements took from soil via root for their metabolisms. On the other hands a several of small organic compounds such as metabolites of soil microorganisms or artificial pollutants also present around plant rhizosphere. Effects of those organic compounds on plants have been unclear. First, we studied on absorption of rhizospheric microbial metabolites by rice plants to clarify a relationship between rhizospheric microorganisms mediated by organic compounds. We clarified rice plant prefers to absorb 2-aminobenzamide that is one of major metabolites of an actynomyces. To clarify a dynamic of absorbed 2-aminobenzamide, we performed purification and Identification of Its derivatives from rice plant. We extracted rice root with methanol to obtained remarked compound named omicron-ABm-1 omicron -ABm-2, omicron-ABzn-3 that could be thought to be metabolites from 2-aminobenzamide. Further spectroscopic analyses of those compounds revealed that omicron-ABm- land 2 should be identified to be N-glucopyranosil-2-aminobenzamide, in detail, omicron-ABm-1 should be beta-anomer and omicron-ABm-2 should be alfa-anomer. Additionally, omicron-ABm-3 should be identified to be N-beta-isomaltosylosil-2-aminobenzamide. Above result appeared that rice plant selectively absorbed 2-aminebenzamide to accumulate it as a corresponding glucoside. We obtained the result that anthranilic acid that is related compound of 2-aminobennzamode be also absorbed by rice plant. However glucosylation efficiency of anthranilic acid in rice was lower than it of 2- aminobeuzamide. We guess that low efficiency of anthranilic acid glucosylation caused by its consumption for tryptophan biosynthesis. It is unclear why rice plant prefer to absorb 2-aminobennzamide and convert it to its corresponding glucoside.
• A basic research for the molecular of plant defense system and an application for agricultre.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 1994 -1995 

  Author : KOBAYASHI Akio; FUKUSAKI Eiichiro; KAJIYAMA Shinichiro

   

  Plants quickly respond to pathogenic invasion. As the result, the secondary metabolism is activated. Therefore, we may use the information obtained from this plant-microbe interaction for secondary metabolite production. For this purpose, we are very much interested in what type of stimulating factors are involved in the activation of the metabolism. We investigated the stimulating factors and we attempted to prepare those, since these eliciting factors can be applicable to produce new secondary metabolites in the plant cell system and a large suply is required. The results of our investigation were listed below. 1) The elicitor activities of deacetylated chitin oligomers to legume plants were investigated. 2) A facile method for preparation of deacetylated chitin oligomers using lysozyme was developed 3) Elicitor-active oligosaccharides from laminaran was purified and thier structures were elucidated. 4) A structure-activity relationship study for elicitor-active oligosaccharide from laminaran was performed.
• Isolation and purification of antifeedant and antioviposition substances against the diamondback moth from a tropical plant.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 1994 -1995 

  Author : NAKASUJI Fusao; KAJIYAMA Shin-ichiro; KOBAYASHI Akio; TSUKUDA Ritsuko; FUJISAKI Kenji

   

  In 1994, antifeedant activity of the acetone extract from A.paniculata against the 1st- and 4th-instar larvae of the diamondback moth (DBM), P.xylostella, was observed at 60 ppm and 500 ppm respectively. Antioviposition effect on the DBM females was also observed on caged cabbage treated with these extracts at 125 ppm. In 1995, a series of fractionation of the aerial parts of A.paniculata guided by a no-choice bioassay test on the 4th-instar larvae of the DBM was conducted for the isolation of the hexane, ETOAc, BuOH and water layrs. Subsequent bioassay tests showed that the hexane layr is the most potent antifeedant against the DBM larvae. Futher fractionation of the hexane layr yielded 7 fractions. Fractions 5 and 6 were still effective even at a reduced dose (50 mug/leaf disc). Thus, fraction 5 was subsequently fractionated and 5 sub-fractions (F5-1 to F5-5) were obtained. Among those F5-2 was the most effective feeding deterrent at a minimum dose of 25mug/leaf disc. Through preparative TLC,an active compound (F526) was finally purified and isolated from F5-2. Further chemical analysis using other analytical procedures will be done to determine the chemical structure of this active compound.
• 紫外レーザを用いた植物生細胞加工技術の開発

  その他の研究制度
• ラン藻細胞外多糖質の構造研究

  その他の研究制度
• 陸生ラン藻の産生する生理活性代謝産物に関する研究

  その他の研究制度
• Development of UV-laser micro processing for plant cells

  The Other Research Programs
• Structure and function of exocellular polysaccharide of cyanobacteria

  The Other Research Programs
• Studies on bioactive compounds of terrestrial blue-green algae

  The Other Research Programs

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