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SHIRAISHI Kohei

    Department of Biotechnology and Chemistry　Professor
Last Updated :2024/04/25

Researcher Information

Degree

  • Doctor of Engineering（1991/03 Osaka City University）
  • Mater of Engineering（1986/03 Osaka City University）

URL

J-Global ID

Research Interests

  • Biomaterial   Functional material   Polymer synthesis   

Research Areas

  • Life sciences / Medical systems
  • Life sciences / Biomaterials
  • Nanotechnology/Materials / Structural and functional materials
  • Nanotechnology/Materials / Polymer chemistry

Academic & Professional Experience

  • 2022/09 - Today  Kindai UniversityGraduate School of Systems Engineering Major in Systems Engineering Biotechnology and Chemistry Course
  • 2009/04 - Today  Kindai UniversityResearch Institute of Fundamental Technology for Next Generation教授
  • 1994/01 - Today  Graduate School of Systems EngineeringMajor in Systems EngineeringProfessor
  • 1994/01 - Today  Kindai Universityシステム工学研究科、システム工学専攻教授
  • 1987 - Today  Kindai UniversityFaculty of Engineering, Department of Biotechnology and Chemistry 教授
  • 1998/08 - 1999/07  Heinrich-Heine Dusseldorf UniversityOrganic Chemistry and Polymer Chemisitury InstitutePost Doctual Fellow

Education

  •        - 1986  Osaka City University  Graduate School of Engineering  応用化学専攻

Association Memberships

  • THE ADHESION SOCIETY OF JAPAN   JAPANESE SOCIETY FOR ENGINEERING EDUCATION   THE SOCIETY OF POLYMER SCIENCE, JAPAN   THE CHEMICAL SOCIETY OF JAPAN   

Published Papers

  • Kikuo Komori; Shinnosuke Takumi; Kiichi Kato; Kazuya Matsumoto; Kohei Shiraishi; Hiroshi Kimura; Kazutake Takada
    Journal of Electroanalytical Chemistry Elsevier BV 943 117621 - 117621 1572-6657 2023/08 [Refereed]
  • Kikuo Komori; Ryo Ihara; Seiryu Hirao; Minghao Liu; Yuki Toyota; Mitsutoshi Nakata; Yuta Tani; Kohei Shiraishi
    Journal of Electroanalytical Chemistry Elsevier BV 922 116704 - 116704 1572-6657 2022/10 [Refereed]
  • Tatsuki Nousou; Seiryu Hirao; Tomohiro Ogawa; Kohei Shiraishi
    Journal of Applied Polymer Science Wiley 139 (22) 52257 - 52257 2022/02 [Refereed]
  • Chie Kojima; Risa Katayama; Thi Lien Nguyen; Yuto Oki; Ayako Tsujimoto; Shin-ichi Yusa; Kohei Shiraishi; Akikazu Matsumoto
    European Polymer Journal Elsevier BV 136 109932 - 109932 0014-3057 2020/08 [Refereed]
     
    © 2020 Elsevier Ltd Copolymers comprising 2-methacryloyloxyethyl phosphorylcholine (MPC) are useful in the production of biomedical devices because of the antifouling effects. As copolymers of MPC with a hydrophobic comonomer are used as a coating agent, their composition, molecular weight, and sequence possibly impact their antifouling effect. In this study, random and block PMDs (rPMDs and bPMDs, respectively) with different compositions were synthesized, and their antifouling effects were examined. Short rPMDs (approximately 50 kDa) with different MPC compositions, from 34 mol% to 85 mol%, were stably coated and suppressed the adhesion of HeLa cells efficiently. rPMDs with 66 mol% and 85 mol% in MPC showed hydrophobic domains at the surface, to which macrophage-like RAW264 cells were adhered. However, rPMDs with 34 mol% and 92 mol% in MPC showed neither hydrophobic domains nor the adhesion of RAW264 cells after the 1-day culture. On the other hand, the antifouling effects of bPMDs were lower than those of rPMDs because of the micelle formation. Therefore, our results suggest that a variety of rPMDs with different MPC contents and different molecular weights can be used as coating agents, which are different from the poly(MPC-co-butyl methacrylate)s used conventionally.
  • Risa Katayama; Nobuyuki Tanaka; Yusuke Takagi; Kohei Shiraishi; Yo Tanaka; Akikazu Matsumoto; Chie Kojima
    Langmuir American Chemical Society (ACS) 36 (20) 5626 - 5632 0743-7463 2020/05 [Refereed]
  • Risa Katayama; Musashi Ikeda; Kohei Shiraishi; Akikazu Matsumoto; Chie Kojima
    Langmuir 35 (37) 12229 - 12235 0743-7463 2019/09 [Refereed]
     
    Copyright © 2019 American Chemical Society. Copolymers comprising 2-methacryloyloxyethyl phosphorylcholine (MPC) and hydrophobic methacrylic esters were used as biomembrane-mimetic polymers to provide blood compatibility. In the present study, we compared the surfaces coated with two MPC polymers with different alkyl groups, namely, poly(MPC-co-butyl methacrylate) (PMB) and poly(MPC-co-dodecyl methacrylate) (PMD), to clarify the effect of their hydrophobic units. Various substrates, such as poly(ethylene terephthalate), polycarbonate, polypropylene, acrylonitrile-butadiene-styrene copolymer, and stainless steel, were coated with ethanol solutions containing various concentrations of PMD or PMB. The solubility of PMD in ethanol changed depending on the water content. Scanning probe microscopy and rhodamine 6G staining revealed heterogeneous microstructures on the PMD-coated surface but not on the PMB-coated surface. Adhesion of various cells was efficiently suppressed by the PMD coating at lower concentration than the PMB coating, except regarding the adhesion of macrophage-like RAW264.7 cells. Our results suggest that the dodecyl groups in PMD increased its affinity for the substrates and simultaneously induced the formation of hydrophobic domains recognized by RAW264.7 cells.
  • Yasue Yamada; Kohei Ohtani; Akinori Imajo; Hanae Izu; Hitomi Nakamura; Kohei Shiraishi
    Toxicology Reports Elsevier Inc. 2 729 - 736 2214-7500 2015/01 [Refereed]
     
    These are many volatile organic compounds (VOCs) that are synthesized, produced from petroleum or derived from natural compounds, mostly plants. Fragrant and volatile organic compounds from plants have been used as food additives, medicines and aromatherapy. Several clinical and pathological studies have shown that chronic abuse of VOCs, mainly toluene, causes several neuropsychiatric disorders. Little is known about the mechanisms of neurotoxicity of the solvents. n-Octanal, nonanal, and 2-ethyl-1-hexanol, which are used catalyzers or intermediates of chemical reactions, are released into the environment. Essential oils have the functions of self-defense, sterilization, and antibiosis in plants. When volatile organic compounds enter the body, there is the possibility that they will pass through the blood-brain barrier (BBB) and affect the central nervous system (CNS). However, the direct effects of volatile organic compounds on neural function and their toxicities are still unclear. We compared the toxicities of n-octanal, nonanal and 2-ethyl-1-hexanol with those of five naturally derived fragrant organic compounds (FOCs), linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and n-phenethyl alcohol. MTT assay of human neuroblastoma SK-N-SH cells showed that the IC< inf> 50< /inf> values of linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and phenethyl alcohol were 1.33, 2.3, > 5, > 5, and 2.39mM, respectively, and the IC< inf> 50< /inf> values of toluene, n-octanal, nonanal and 2-ethyl-1-hexanol were 850, 37.2, 8.31 and 15.1μM, respectively. FOCs showed lower toxicities than those of VOCs. These results indicate that FOCs are safer than other compounds.
  • Tatsuki Nousou; Akinori Imajo; Kohei Shiraishi
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 72 (6) 354 - 360 0386-2186 2015 [Refereed]
     
    Thermoresponsive polymer patterned-immobilized cell microarrays can be used as stem cell diagnosis and non-invasive recovery devices. In order to develop such an array, we grafted poly(N-isopropylacrylamide) (PNiPAAm) with controlled graft-PNiPAAm chain length and density on a glass substrate by the activator regenerated by electron transfer (ARGET)/atom transfer radical polymerization (ATRP) method. The grafting density was controlled by the concentration of 2-bromoisobutylbromide as a reagent on amino-functionalized glass. The grafted PNiPAAm chain length could also be controlled by the polymerization time. Cell attachment and thermal stimuli-exfoliation of immortal mesenchymal stem cells (hiMSCs) as a model stem cell was examined on such surfaces. The amount of hiMSCs attached decreases by increasing grafted PNiPAAm chain length. The thermal stimuli-exfoliation of attached hiMSC by cooling to 15 degrees C for 30 min exceeded 80%. The recovery of a hiMSC sheet by the thermal stimuli-exfoliation was observed on the PNiPAAm-g-glass with a PNiPAAm layer thickness of 19.3 nm.
  • Tatsuki Nousou; Akinori Imajo; Kohei Shiraishi
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 72 (11) 667 - 672 0386-2186 2015 [Refereed]
     
    In order to use a poly(-phenyl-2-(trimethylsilyl)acetylene) [PTMSDPA] O-2 enrichment membrane as a cell culture substrate, it was irradiated with an Ar plasma and grafted with poly(N-isopropylacrylamide) [PNiPAAm]. The average amount of grafted PNiPAAm was 1.4 0.7 X 10-2 mg cm(-2), and the degree of graft PNiPAAm was 29 +/- 13. The contact angle (9) of water at 25 C after treatment decreased from 9 = 90 for the untreated PTMSDPA to theta= 48 degrees. HeLa cells attached and proliferated on the grafted polymer (g-PTMSDPA) at 37 degrees C, above the lower critical solution temperature of g-PTMSDPA. After the cultivation of HeLa cells, the thermal stimuli -exfoliation was also examined at 20 degrees C. The ratio of number of thermal stimuli -exfoliation cells to the total number of attached HeLa cells of 3.3 0.3 X 104 cells cm(-2) on g-PTMSDPA was about 28 0.9%, while little thermal stimuli -exfoliation was observed in the case of untreated PTMSDPA. The effect of 02 -supply on cell proliferation was also evaluated by supplying O-2 from the back side of the g-PTMSDPA film at 0.01 MPa, with a flow rate of 20 sccm, for 24h. The number of HeLa cells and SK-N-SH cells growth under O-2-supply were about 1.4 and 1.3 times higher than that without O-2-supply, respectively.
  • 白石浩平; 相良宗作; 杉山一男; 矢野 徹; 阪口敬子; 橋本邦彦
    機能材料 シーエムシー出版 33 (8) 36 - 41 0286-4835 2013/08 [Refereed]
  • Kohei Shiraishi; Sinko Wakisaka; Junji Satozaki; Kazuo Sugiyama
    Kobunshi Ronbunshu SOC POLYMER SCIENCE JAPAN 69 (1) 39 - 46 0386-2186 2012/01 [Refereed]
     
    In order to develop a novel biomaterial showing antithrombogenisity, we prepared zwitterionic poly [N-α-acrylamide-L-lysine] [poly (α-LysAA)] or poly[N-α-methacrylamide-L-lysine] [poly-(α- LysMA)] with a selective binding activity to fibrinolytic proteins [plasminogen (Plg), tissue-type plasminogen activator (t-PA)]. Surface Plasmon Resonance (SPR) measurements on poly(α-LysAA) immobilized sensor surfaces found that Plg and t-PA were strongly bound to poly(α-LysAA) for Plg with 3.89 × 10 -7 M -1 for t-PA with 1.55 × 10 -7 M -1 as a binding constant, respectively, while other serum proteins such as albumin, γ-globulin, and fibrinogen were weakly bound. The competitive adsorption of albumin and Plg on the immobilized polymer was also carried out. A specific interaction was found between poly(±-LysAA) and Plg. The maximam rate constant for the hydrolysis of chromogenic plasmin substrate (S-2251) by Plg/t-PA in the presence of poly(α-LysAA) was found to be V max = 20.0 × 10 -3 mM · min -1. The enhanced enzymatic reaction of Plg/t-PA and S-2251 was also observed in the presence of poly(α-LysAA) or poly(α-LysMA). © 2012, The Society of Polymer Science, Japan.
  • Blood Compatibility of Amphiphilic Poly(N-a-acrylamide-L-lysine-b-dimethylsioxane)Block Copolymers
    SHIRAISHI Kohei
    Journal of Biomaterials and Nanobiotechnology 2 337 - 346 2011 [Refereed]
  • Ryoji Hamawaki; Tatsuya Ishihara; Akihiro Tominaga; Kohei Shiraishi; Kazuo Sugiyama; Yuki Nitta; Tatsuyuki Nakatani; Keishi Okamoto
    JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY TECHNICAL ASSOC PHOTOPOLYMERS,JAPAN 24 (4) 447 - 452 0914-9244 2011 [Refereed]
     
    Two-step surface modification of metal or glass substrates has been developed for the long-term reliability and safety of biomedical devices (stents, catheters, blood contacting parts,etc) and cell microarrays for cell diagnosis and cell recovery. First step, carboxylic group (COOH)s containing DLC (DLC/COOH) were coated on an aluminum surface by a plasma enhanced chemical vapor deposition (PECVD). Second step, an anti-thrombogenic, poly(N-alpha-(methacrylamide-L-lysine) (PLysMA) or poly[2-(methacryloyloxy)ethyl phosphorylcholine] (PMPC) was grafted on the DLC/COOH surface by plasma irradiation-post polymerization technique.Furthermore, to develop novel cell microarrays having regulatory-arranged glass spots and coated the surface with DLC/COOH around the spots for evaluation of cell function and selection of targeting cells by immobilization of single cells on the spots, the microarrays were also grafted with PMPC on the DLC/COOH by the plasma irradiation-post polymerization technique. Surface biocompatibility and characterization were examined by SPM, XPS, and adsorption of plasma proteins.
  • Chikako Ishida; Shigeru Ueshima; Nobuo Nagai; Naoyuki Kawao; Kiyotaka Okada; Hou Yongzhong; Kohei Shiraishi; Osamu Matsuo
    BLOOD COAGULATION & FIBRINOLYSIS LIPPINCOTT WILLIAMS & WILKINS 21 (3) 272 - 278 0957-5235 2010/04 [Refereed]
     
    The fibrinolytic activity of blood is regulated by expressing tissue-type plasminogen activator (t-PA) and its specific inhibitor, type-1 plasminogen activator inhibitor (PAI-1), from vascular endothelial cells. Since t-PA is a major plasminogen activator in blood, it is considered that the binding protein for t-PA, which exists on endothelial cell membrane, immobilizes t-PA on the surface of endothelial cells and enhances their antithrombotic property. Recently, we have found a new t-PA binding protein in endothelial cells. Its amino acid sequence has matched that of human adenine nucleotide translocase-1 (ANT1). The aims of this study are to confirm the binding of t-PA to ANT1, and to clarify the effect of ANT1 on fibrinolytic activity around endothelial cells. ANT1 is prepared from recombinant glutathione S-transferase (GST)-ANT1 fusion protein, and reveals t-PA binding activity in a ligand blot assay. In addition, ANT1 is exclusively expressed on endothelial cell membrane by using pDisplay vector. Interaction of t-PA with ANT1, which is expressed on the surface of endothelial cells, is confirmed by IAsys binding analysis and chromogenic assay. The heterologous expression of ANT1 on endothelial cell membrane enhances the t-PA binding ability of endothelial cells and the effect of ANT1 expression on fibrinolytic activity is demonstrated by increasing t-PA-catalyzed plasminogen activation. These results suggest that a novel t-PA-binding protein, ANT1, may concentrate t-PA on the surface of cells and enhance fibrinolytic properties around endothelial cells; therefore, ANT1 can be a powerful tool for regulating the plasminogen activation system in the vessel. Blood Coagul Fibrinolysis 21:272-278 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
  • SHIRAISHI Kohei; SAGARA Syusaku; HARADA Dai; SUGIYAMA Kazuo; YANO Toru; SAKAGUCHI Keiko
    Journal of The Adhesion Society of Japan The Adhesion Society of Japan 47 (4) 131 - 137 0916-4812 2010 [Refereed]
     
    To give properties of both high heat resistance and impact strength，which are required for Industrial components，to poly-L-lactic acid (PLA)，we sought to create a compound that includes an agent granting flexibility，a nucleating agent of proprietarily developed starch and poly-D-lactic acid. We also used natural rubber as a flexible phase，and epoxidizednatural rubber，which joins the flexible and rigid crystalline phases，and poly(carbodiimide) (PCDI) as a hydrolysis inhibitor and anadhesionbetween NR/ENR and PLA to create a new compound for injection molding that uses morethan 97% plant material. Compared to PLA itself，the injection molding products obtained from the modified PLA compound (97% plant material) had an increased heat resistance of about 50℃ and 10 times greater impact strength from the evaluation of the impact and thermal mechanical properties of the compounds by JIS K7160 and JIS K7191-2Be. The physical improvements in PLA are due to the bonding of the uniformly distributed PLA crystalline phase by scanning probe microscopy. The effect of molecular weight of PCDI or amount of PCDI added was also examined on their mechanical properties or PLA crystallization rate.
  • Nobuyuki Tanigawa; Kohei Shiraishi; Tadashi Abe; Kazuo Sugiyama
    JOURNAL OF APPLIED POLYMER SCIENCE JOHN WILEY & SONS INC 113 (2) 959 - 965 0021-8995 2009/07 [Refereed]
     
    Poly [2-(methlacryloyloxy)ethyl phosphorylcholine](PMPC) with one pendant tocopheryl moiety at the polymer terminus (PMPC-Toco) was prepared by the radical polymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) initiated with 4,4'-azobis[(3-tocopheryl)-4-cyanopentanoate] in the presence of 2-mercaptoethanol as a chain transfer reagent. The self-organization of PMPC-Toco was analyzed with fluorescence and (1)H-NMR measurements. The critical micelle concentrations of PMPC-Toco with [eta] = 0.25, 0.13, 0.1.0, and 0.05 dL g(-1) were found to be 200, 100, 100, and 90 mg L(-1), respectively. The blood compatibility of PMPC-Toco was evaluated from the Michaelis constant (K for the enzymatic reaction of thrombin and a synthetic substrate, S-2238, in the presence of PMPC-Toco. The K values were 0.21, 0.23, 0.36, and 0.21 for PMPC-Toco-1, 2, 3, and PMPC ([eta] = 0.56 dL g(-1)), respectively. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 959-965, 2009
  • Improvement of physical properties of poly(lactic acid) by compounding natural rubber
    白石 浩平; 相良宗作; 杉山一男; 矢野 徹; 阪口敬子
    高分子(HOT TOPICS) 57 (12) 962  2008/12 [Refereed]
  • 白石 浩平; 相良宗作; 杉山一男; 矢野 徹; 阪口敬子; 橋本邦彦
    Plastics age プラスチックス・エ-ジ 54 (12) 97 - 102 0551-0503 2008/12 [Refereed]
  • Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    Kobunshi Ronbunshu 65 (2) 150 - 156 0386-2186 2008/02 [Refereed]
     
    Block copolymers, poly (LysMA-b-DMS)-1-3 with different molecular weight, were prepared by the conversion of poly[N-α-methacrylamide-N-ε-t-butoxycarbonyl-L-lysin-t-butyl ester (BLMA)-b-dimethylsiloxane (DMS)] which were obtained from the radical polymerrization of BLMA initiated with an azo-initiator having poly-dimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly(LysMA-b-DMS) showed microphase-separated structures. The self-organization of poly(LysMA-b-DMS) was analyzed by fluorescence and 1H NMR measurements. The critical micelle concentrations (CMC) were = 500-2000 ing L-1. The micelle had diameters from 221 to 274 nm. The blood compatibility of poly (LysMA-b-DMS) was evaluated from the maximum velocity of hydrolysis (Vmax) for the enzymatic reaction of thrombin and a synthetic substrate S-2238 in the presence of poly(LysMA-b-DMS). Vmax was 1.82 × 10-2 - 1.95 × 10-2 mM min-1. The maximum rate of conversion of plasminogen to plasmin by tissue plasminogen activator (t-PA) in the presence of poly(LysMA-b-DMS) using S-2251 was faster than the case of absence of poly(LysMA-b-DMS).
  • Kohei Shiraishi; Tetsuyuki Shigesada; Kazuhiko Yamamoto; Kazuo Sugiyama
    Kobunshi Ronbunshu 65 (2) 132 - 139 0386-2186 2008/02 [Refereed]
     
    In order to develop a more precise evaluation method for the application as a cell scaffold, the surface of thermoresponsible poly (N-isopropylacrylamide) (PNiPAAm) grafted PET (g-PET) was characterized by a bacterial random peptide library method (FliTrx™: Invitrogen). The g-PET was prepared by an Ar plasma-post polymerization method. From the analysis of selective 12 amino acids sequences, we could observe that the surface of g-PET was hydrophilic at 25°C and hydrophobic surface at 37°C, respectively. In addition, the Kyte & Doolittle parameter of g-PET was - 67.6 on hydrophilic surface at 25°C and - 28.4 for the hydrophobic surface at 37°C. On the other hand, the Kyte & Doolittle parameter of the untreated PET was 34.3 at 25°C and 42.2 at 37°C, respectively. The Selection of amino acids sequences changed drastically on the g-PET by raising the panning temperature from 25°C to 37°C.
  • Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 65 (2) 150 - 156 0386-2186 2008 [Refereed]
     
    Block copolymers, poly (LysMA-b-DMS)-1 similar to 3 with different molecular weight, were prepared by the conversion of poly[N-alpha-methacrylamide-N-epsilon-t-butoxycarbonyl-L-lysin-t-butyl ester (BLMA)-b-dimethylsiloxane (DMS)] which were obtained from the radical polymerrization of BLMA initiated with an azo-initiator having poly-dimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly (LysMA-b-DMS) showed microphase-separated structures. The self-organization of poly (LysMA-b-DMS) was analyzed by fluorescence and H-1 NMR measurements. The critical micelle concentrations (CMC) were = 500-2000 mg L-1. The micelle had diameters from 221 to 274 nm. The blood compatibility of poly (LysMA-b-DMS) was evaluated from the maximum velocity of hydrolysis (V-max) for the enzymatic reaction of thrombin and a synthetic substrate S-2238 in the presence of poly (LysMA-b-DMS). V-max was 1.82 x 10(-2)similar to 1.95 x 10(-2) mM min(-1). The maximum rate of conversion of plasminogen to plasmin by tissue plasminogen activator (t-PA) in the presence of poly (LysMA-b-DMS) using S-2251 was faster than the case of absence of poly (LysMA-b-DMS).
  • Kohei Shiraishi; Tetsuyuki Shigesada; Kazuhiko Yamamoto; Kazuo Sugiyama
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 65 (2) 132 - 139 0386-2186 2008 [Refereed]
     
    In order to develop a more precise evaluation method for the application as a cell scaffold, the surface of thermoresponsible poly (N-isopropylacrylamide) (PNiPAAm) grafted PET (g-PET) was characterized by a bacterial random peptide library method (FliTrx (TM): Invitrogen). The g-PET was prepared by an Ar plasma-post polymerization method. From the analysis of selective 12 amino acids sequences, we could observe that the surface of g-PET was hydrophilic at 25 degrees C and hydrophobic surface at 37 degrees C, respectively. In addition, the Kyte & Doolittle parameter of g-PET was -67.6 on hydrophilic surface at 25 degrees C and -28.4 for the hydrophobic surface at 37 degrees C. On the other hand, the Kyte & Doolittle parameter of the untreated PET was 34.3 at 25 degrees C and 42.2 at 37 degrees C, respectively. The Selection of amino acids sequences changed drastically on the g-PET by raising the panning temperature from 25 degrees C to 37 degrees C.
  • Sayuri Otaki; Shigeru Ueshima; Kohei Shiraishi; Kazuo Sugiyama; Suguru Hamada; Masatomo Yorimoto; Osamu Matsuo
    CELL BIOLOGY INTERNATIONAL ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD 31 (10) 1191 - 1197 1065-6995 2007/10 [Refereed]
     
    The technique of tissue engineering is developing for the restoration of lost tissues. This new technique requires cells that fabricate tissue. Mesenchymal stem cells in bone marrow have been used as the cell source for this technique; however, dental pulp cells have recently been shown to possess stem-cell-like properties. We earlier demonstrated that dental pulp cells proliferate and produce an extracellular matrix that subsequently becomes mineralized in vitro. We now report that such dental pulp cells (first to eighth passage) produced bone instead of dentin when those cells were implanted into subcutaneous sites in immunocompromised mice with HA/TCP powder as their carrier. This evidence shows that dental pulp cells are the common progenitors of odontoblasts and osteoblasts, or dental pulp cells are mesenchymal stem cells themselves. It is expected that dental pulp cells can be a useful candidate cell source for tissue engineering, and contain the potential of new therapeutic approaches for the restoration of damaged or diseased tissue. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
  • Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    Kobunshi Ronbunshu 64 (6) 373 - 379 0386-2186 2007/06 [Refereed]
     
    Block copolymers, poly (MPC-b-DMS)-I∼IV, were prepared by the radical polymerization of 2-(methacryloyloxy) ethyl phosphorylcholine (MPC) initiated with an azo-initiator having polydimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly (MPC-b-DMS) was found to form microphase- separated structures. The self-organization of poly(MPC-b-DMS) was analyzed by fluorescence and 1H NMR measurements. The critical micelle concentrations of poly (MPC-b-DMS) were 90-600 mg L-1. The blood compatibility of poly(MPC-b-DMS)-I∼IV was evaluated from the Michaelis constant (Km) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238. Km was found to be 0.10-0.16 mM.
  • Kohei Shiraishi; Minoru Sugiyama; Yoji Okamura; Kazuo Sugiyama
    JOURNAL OF APPLIED POLYMER SCIENCE JOHN WILEY & SONS INC 103 (5) 3064 - 3075 0021-8995 2007/03 [Refereed]
     
    Poly[9-(2-methacryloyloxyethyl)adenine] and poly[1-(2-methacryloyloxyethyl)thymine] with one pendant cholesteryl moiety at the polymer end (PMEA-Chol, PMET-Chol) and with two pendant cholesteryl moieties at both polymer ends as terminal groups (PMEA-2Chol, PMET-2Chol) were prepared by radical polymerization of 9-(2-methacryloyl-oxyethyl)adenine (MEA) and 1-(2-methacryloyloxyethyl)thymine (MET) initiated with 4,4'-azobis[(3-cholesteryl)4-cyanopentanoate] in the presence of 2-mercaptoethanol or thiocholesterol as chain transfer reagents, respectively. The copolymers [PNiPAAm-co-PMEA-nChol (n = 1,2)] composed of N-isopropylacrylamide (NiPAAm) and MEA were also prepared in a similar manner. The self-organization of these polymers and copolymers was confirmed by a fluorescence measurement, and then their critical concentrations of micelle formation (CMC) were determined. The mixture of PMEA-2Chol and cholesterol as a lipophilic drug model formed a lamella type of complex with an interplaner spacing of d = 35.3 angstrom. The hypochromism based on the formation of a 1 : I interaction of adenine and thymine moieties was found to appear in the mixed aqueous solution of PMEA-Chol and PMET-Chol. Complementary interactions were also confirmed in the system of PMET-2Chol and adenosine as well as PMEA-2Chol and uridine. Cis-dichlorodiammine platinum(II) (CDDP) was bound to PNiPAAm-co-PMEA-Chol through the adenine moiety by ligand substitution atoms of CDDP. The amount of CDDP loaded on the copolymer was found to be 0.143 g g(-1). (c) 2006 Wiley Periodicals, Inc.
  • Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 64 (6) 373 - 379 0386-2186 2007 [Refereed]
     
    Block copolymers, poly (MPC-b-DMS)-I-IV, were prepared by the radical polymerization of 2-(methacryloyloxy) ethyl phosphorylcholine (MPC) initiated with an azo-initiator having polydimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly(MPC-b-DMS) was found to form microphase-separated structures. The self-organization of poly(MPC-b-DMS) was analyzed by fluorescence and H-1 NMR measurements. The critical micelle concentrations of poly (MPC-b-DMS) were 90-600 mg L-1. The blood compatibility of poly (MPC-b-DMS) -I-IV was evaluated from the Michaelis constant (K-m) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238. K-m was found to be 0.10-0.16 mM.
  • Kohei Shiraishi; Megumi Ohdan; Kazuma Maeda; Kazuo Sugiyama; Katsuyuki Suzuki; Hiroshi Hosoya
    Kobunshi Ronbunshu 63 (9) 613 - 620 0386-2186 2006/09 [Refereed]
     
    In order to develop a novel cell cultured material capable of thermal stimulating cell detachment from its surface, we studied the biocompatible and thermosensitive copolymer consisting of N-(2-hydroxypropyl) methacrylamide (HPMA) and methyl methacrylate (MMA) [poly(HPMA-co-MMA): HPMA/MMA = 100/27.6 (molar ratio)]. With N-isopropylacylamide (NiPAAm) as a control of thermosensitive polymer, the copolymers were grafted onto poly (ethyleneterephthalate) (PET) by Ar plasma irradiation-post polymerization technique. From the contact angle (θ) measurements to water at 25°C, the θ values of poly (HPMA-co-MMA) grafted PET (g-PET-I: amount of grafted polymer = 0.125 mg cm-2) and poly (NiPAAm) grafted PET (g-PET-II: 0.109 mg cm-2) decreased from θ=80° (the original PET) to θ=52° and θ=50°, respectively. HeLa cells attached and proliferated on g-PET-I and g-PET-II at 37°C, above the lower critical solution temperature of poly (HPMA-co-MMA). After the cultivation of HeLa cells, the thermal stimuli-exfoliation was also examined at 4°C for 2 h. The ratios of the number of thermal stimuli-exfoliation cells to the total number of attached cells on g-PET-I or g-PET-II were 73% and 45%, respectively, while little thermal stimuli-exfoliation was observed in the case of untreated PET.
  • Laser Sintering of Stainless Steel Using Resin Powder
    京極 秀樹; 白石 浩平
    Solid Freeform Fabrication Proceedings(CD-ROM) 2006/05
  • Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    Zairyo/Journal of the Society of Materials Science, Japan 55 (4) 391 - 396 0514-5163 2006/04 [Refereed]
     
    Block copolymers, Poly (HPMA-b-DMS)-1-7, were obtained from polymerization of N-(2-hydroxypropyl) methacrylamide (HPMA) initiated with polydimethylsiloxane (PDMS) type of azo-initiator [4,4-azobis (polydimethylsiloxane)-4-cyanopentanoate (azo-PDMS)]. From XPS measurements of freeze dried samples, the hydrophilic PHPMA segment was found to migrate to the surface of Poly (HPMA-b-DMS) films in water. Poly (HPMA-b-DMS) showed lower contact angle (θ= 54°-100°) than PDMS itself (θ= 103°). It was also found that the contact angle of block copolymers was changed in response to change in temperature, i.e., θ= 64°-79° at 40°C and θ= 54°-69° at 20°C for Poly (HPMA-b-DMS). The amount of adsorption of albumin on the Poly (HPMA-b-DMS) film was depressed at 20°C compared to the case at 40°C. For the purpose of an application to a thermo-responsive drug delivery system, copolymer hydrogel Gel (HPMA-b-DMS) was also prepared by radical copolymerization of HPMA and N, N'-ethylenebisacrylamide initiated with azo-PDMS.
  • Kohei Shiraishi; Megumi Ohdan; Kazuma Maeda; Kazuo Sugiyama; Katsuyuki Suzuki; Hiroshi Hosoya
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 63 (9) 613 - 620 0386-2186 2006 [Refereed]
     
    In order to develop a novel cell cultured material capable of thermal stimulating cell detachment from its surface, we studied the biocompatible and thermosensitive copolymer consisting of N-(2-hydroxypropyl)methacrylamide(HPMA) and methyl methacrylate(MMA) [poly(HPMA-co-MMA): HPMA/MMA = 100/27.6 (molar ratio)]. With N-isopropylacylamide (NiPAAm) as a control of thermosensitive polymer, the copolymers were grafted onto poly (ethyleneterephthalate) (PET) by Ar Plasma irradiation-post polymerization technique. From the contact angle (theta) measurements to water at 25 degrees C, the theta values of poly (HPMA-co-MMA) grafted PET (g-PET-1: amount of grafted polymer = 0.125 mg cm(-2)) and poly (NiPAAm) grafted PET (g-PET-II: 0.109 mg cm(-2)) decreased from theta = 80 degrees (the original PET) to theta = 52 degrees and theta = 50 degrees, respectively. HeLa cells attached and proliferated on g-PET-I and g-PET-II at 37 degrees C, above the lower critical solution temperature of poly (HPMA-co-MMA). After the cultivation of HeLa cells, the thermal stimuli-exfoliation was also examined at 4 degrees C for 2 h. The ratios of the number of thermal stimuli-exfoliation cells to the total number of attached cells on g-PET-I or g-PET-II were 73% and 45%, respectively, while little thermal stimuli-exfoliation was observed in the case of untreated PET.
  • 白石 浩平; 山本 和彦
    化学と工業 58 (3) 237 - 238 0022-7684 2005/03 
    両性イオン構造のL-Lysを側鎖にもつ人工高分子の線溶機能の利用を目指す人工高分子材料としての機能と可能性について解説した。
  • K Shiraishi; M Kohta; K Sugiyama
    CHEMISTRY LETTERS CHEMICAL SOC JAPAN 33 (6) 646 - 647 0366-7022 2004/06 [Refereed]
     
    Zwitterionic polymethacrylamides, poly(N-alpha-methacrylamide-L-lysine) [poIy(alpha-LysMA)] and poly(N-epsilon-methacrylamide-L-lysine) [poly(epsilon-LysMA)] were prepared to examine their bioactivity by an evaluation of fibrinolytic activity and a binding assay using resonant mirror biosensor (IAsys). Poly(alpha-LysMA) enhanced the fibrinolytic activity by plasminogen/tissue-type plasminogen activator while no enhancement was observed in the case of poly(epsilon-LysMA). A strong interaction between Pig to poly(alpha-LysMA) was also observed by IAsys when compared with poly(epsilon-LysMA).
  • K Sugiyama; K Shiraishi; K Sono
    JOURNAL OF APPLIED POLYMER SCIENCE JOHN WILEY & SONS INC 90 (1) 143 - 148 0021-8995 2003/10 [Refereed]
     
    Surface-modified polypropylene (PP) films with thermally and photochemically sensitive copolymers consisting of N-(2-hydroxypropyl)methacrylamide (HPMA) and 4-(4-methoxvphenylazo)phenyl methacrylate (MPAP), poly(HPMA-co-MPAP)-g-PP (abbreviated g-PP) film, were prepared by graft copolymerization with an Ar-plasma postpolymerization technique. The surfaces of the g-PP films were characterized by means of X-ray photoelectron spectroscopy; the percentage grafting of poly(HPMA-co-MPAP) with a number-average molecular weight of 3.28 x 10(4) was 7.12%, and the molar ratio of HPMA-MPAH in the copolymer was 0.75:0.25. The stimuli-sensitive adsorption of albumin and polystyrene microspheres on the g-PP film was also measured. (C) 2003 Wiley Periodicals, Inc.
  • K Sugiyama; K Shiraishi; T Matsumoto
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY JOHN WILEY & SONS INC 41 (13) 1992 - 2000 0887-624X 2003/07 [Refereed]
     
    Poly[2-(methacryloyloxy)ethyl phosphoryleholine]s (PMPCs) with one pendant cholesteryl moiety at the polymer end (PMPC-Chol-I and PMPC-Chol-II) and two pendant cholesteryl moieties at both polymer ends as terminal groups (PMPC-2Chol-I and PMPC-2Chol-II) were prepared by the radical polymerization of 2-(methacryloyloxy)ethyl phosphorylcholine initiated with 4,4'-azobis[(3-cholesteryl)-4-cyanopentanoate] in the presence of 2-mercaptoethanol or thiocholesterol as chain-transfer reagents, respectively. The self-organization of PMPC-Chol and PMPC-2Chol was analyzed with fluorescence and H-1 NMR measurements. The critical micelle concentrations of PMPC-Chol-I with a degree of polymerization (P-n) of 91 and of PMPC-2Chol-I with a P-n value of 165 were 250 and 27 mg L-1, respectively. The blood compatibility of PMPC-2Chol was evaluated from the Michaelis constant (K-m) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238, in the presence of PMPC-2Chol. K-m was 0.07, 0.05, and 0.56 for PMPC-2Chol-I with P-n = 165, PMPC-2Chol-II with P-n = 38, and PMPC (an intrinsic viscosity of 0.54 dL g(-1)) initiated with 2,2'-azobisisobutyronnitrile in the absence of chain transfer agent, respectively. A mixture of PMPC-2Chol-II and cholesterol as a drug. model formed a lamellar type of complex with an interplanar spacing of d = -35.2 Angstrom. (C) 2003 Wiley Periodicals, Inc.
  • SHIRAISHI Kohei; MATSUDA Hisashi; OHASHI Toshihiko; YOSHIMITSU Yoshikuni; SUGIYAMA Kazuo
    Journal of the Society of Materials Science, Japan The Society of Materials Science, Japan 52 (3) 319 - 324 0514-5163 2003/03 [Refereed]
     
    Two series of copolymers, Poly (RAAm-co-ST) and Poly (BMAAm-co-R`MA), as the novel primers in adhesive bonding polypropylene (PP) were prepared by radical copolymerization of alkoxymethyl acrylamide (RAAm) and styrene (ST) or alkyl methacrylate (R`MA). Alkoxy groups (OR) in the RAAm moieties used were methoxy, ethoxy, isobutoxy and butoxy, whereas alkyl groups (R`) in the R`MA moieties were methyl, ethyl, isopropyl, isobutyl, n-butyl and n-hexyl. Homopolymer of RAAm, Poly (RAAm), was also prepared and characterized : the glass transition temperature of Poly (RAAm) was T_g=76.8∿70.0℃ and Poly (RAAm) was thermally decomposed through a two step degradation with the evolution of corresponding alcohol. The bonding properties of PP, using two series of copolymers as primers, were studied by measuring the shear strength (τ) of single-lap joints between PP and PP or other substrates such as polyethyleneterephthalate (PET), nylon 66 (66N), alminium, steel and glass. The primers tested were effective when used with polyurethane adhesive. Mixed substrate bonds prepared between PP and others gave substantial bond strength (τ=48.4∿51.3 MPa) in comparison to bond strength for unprimered controls (τ=8.9∿16.7 MPa). Maximum bond strength was obtained when Poly (BMAAm-co-HMA) was chosen as a primer. The mechanism for the adhesive bonding of PP was discussed.
  • Kohei Shiraishi; Koji Miura; Go Asami; Masushi Kohta; Kazuo Sugiyama
    Kobunshi Ronbunshu Society of Polymer Science 60 (1) 30 - 37 0386-2186 2003 [Refereed]
     
    The amphoteric polymer having L-serine residues in the side chain as a pH responsible hydrogel component was prepared as follows: L-serine with protected amino and carboxyl groups, N -tert-butoxycarbonyl-O -methacryloyl-L-serine diphenylmethyl ester (Boc-SerMA-Ph2) and 2-(dansyloxy) ethyl methacrylate (DnsEMA) were copolymerized by a radical mechanism with 2,2' -dimethyl-azobisisobutyrate in tetrahydrofuran at 60 °C for 20 h. Deprotection of the resulting polymer was carried out in 80% trifluoroacetic acid aqueous solution to give amphoteric poly(SerMA labeled with a dansyl group [poly(SerMA-co-DnsEMA)]. The fluorescence spectral changes of a dansyl-labeled poly(SerMA) solution at pH ranging from 1.2 to 12.0 revealed that poly(SerMA) shows pH response. The interaction between a serum protein such as bovine serum albumin (Alb), human γ-globulin (Glo), or bovine serum fibrinogen (Fib), and poly(SerMA-co-DnsEMA) was also examined by means of fluorescence spectroscopy in a phosphate buffered solution at pH 5.7 for Alb and Fib, pH 6.2 for Glo. The interaction between Alb and poly(SerMA-co-DnsEMA) was observed, whereas no definite interaction was confirmed for Glo and Fib.
  • K Shiraishi; K Miura; G Asami; M Kohta; K Sugiyama
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 60 (1) 30 - 37 0386-2186 2003 [Refereed]
     
    The amphoteric polymer having L-serine residues in the side chain as a pH responsible hydrogel component was prepared as follows: L-serine with protected amino and carboxyl groups, N-tert-butoxycarbonyl-O-methacryloyl-L-serine diphenylmethyl ester (Boc-SerMA-Ph-2) and 2-(dansyloxy) ethyl methacrylate (DnsEMA) were copolymerized by a radical mechanism with 2,2'-dimethyl-azobisisobutyrate in tetrahydrofuran at 60 degreesC for 20 h. Deprotection of the resulting polymer was carried out in 80% trifluoroacetic acid aqueous solution to give amphoteric poly(SerMA) labeled with a dansyl group [poly(SerMA-co-DnsEMA)]. The fluorescence spectral changes of a dansyl-labeled poly(SerMA) solution at pH ranging from 1.2 to 12.0 revealed that poly(SerMA) shows pH response. The interaction between a serum protein such as bovine serum albumin (Alb), human gamma-globulin (Glo), or bovine serum fibrinogen (Fib), and poly(SerMA-co-DnsEMA) was also examined by means of fluorescence spectroscopy in a phosphate buffered solution at pH 5.7 for Alb and Fib, pH 6.2 for Glo. The interaction between Alb and poly(SerMA-co-DnsEMA) was observed, whereas no definite interaction was confirmed for Glo and Fib.
  • K Sugiyama; S Rikimaru; Y Okada; K Shiraishi
    JOURNAL OF APPLIED POLYMER SCIENCE JOHN WILEY & SONS INC 82 (1) 228 - 236 0021-8995 2001/10 [Refereed]
     
    Amphiphilic random copolymers, poly(R-HPMA-co-S-PAM) and poly(H-PMA-co-S-PAM), were prepared by radical copolymerization of N-methacryloyl-(S) phenylalanine methyl ester (S-PAM) and N- [(R)-2-hydroxypropyl] methacrylamide (R-HPMA) or N-(2-hydroxypropyl)methacrylamide (HPMA) with various molar ratios of R-HPMA (or HPMA) (m) to S-PAM (n). Either aqueous solution of poly(R-HPMA-co-S-PAM) with the molar ratio of m : n = 0.81 : 0.19 or poly(HPMA-co-S-PAM) with the molar ratio of m : n = 0.79 : 0.21 exhibited the lower critical solution temperature (LCST) at 16 degreesC. The LCST in the presence of (S)-(-)-phenylalanine (S-Phe) shifted from 16 to 20 degreesC and 18 degreesC for poly(R-HPMA- co-S-PAM) and poly(HPMA-co-S-PAM), respectively, whereas the LCST did not shift in the presence of(R-( +)-phenylalanine (R-Phe). Thermosensitive Gel(R-HPMA-co-S-PAM) and Gel(HPMA-co-S-PAM) were also prepared from radical copolymerization of S-PAM and R-HPMA or HPMA in the presence of N,N ' -ethylenebisacrylamide (EBAAm) as a crosslinker. When the gels shrunk at 40 degreesC, the release of dansyl-(R)-phenylalanine (Dans-R-Phe) from the gel in which loaded Dans-R-Phe occurred was more easily done than that of Dans-S-Phe from the gel that loaded Dans-S-Phe. Thus, these thermosensitive copolymers and gels were found to exhibit chiral recognition for phenylalanine derivatives. (C) 2001 John Wiley & Sons, Inc.
  • K Sugiyama; K Shiraishi; K Okada; O Matsuo
    POLYMER JOURNAL SOC POLYMER SCIENCE JAPAN 31 (10) 883 - 886 0032-3896 1999 [Refereed]
  • K Shiraishi; T Ohnishi; K Sugiyama
    MACROMOLECULAR CHEMISTRY AND PHYSICS WILEY-V C H VERLAG GMBH 199 (9) 2023 - 2028 1022-1352 1998/09 [Refereed]
     
    Poly(methyl methacrylate) microspheres [poly(SerMA-co-MMA), poly(AlaMAm-co-MMA), poly(AlaEMA-co-MMA, and poly(AlaMAm/AlaEMA-co-MMA)] modified with O-methacryloyl-L-serine (SerMA), N-methacryloyl-L-alanine (AlaMAm) and L-alanine 2-methacryloyloxyethyl ester (AlaEMA) were prepared by the emulsifier-free emulsion copolymerization of methyl methacrylate (MMA) with SerMA, AlaMAm and AlaEMA, respectively, initiated with 2,2'-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (ABIP). The series of polymer microspheres showed unimodal distribution of the particle size (290-800 nm) in water. From X-ray photoelectron spectroscopy it was concluded that the amino acid moieties are located on the surface of the particles in all cases. Poly(SerMA-co-MMA) showed the most effective suppression of adsorption of proteins such as albumin (Alb), gamma-globulin (Glo) and fibrinogen (Fib) among the examined poly(methylmethacrylate) microspheres.
  • K Sugiyama; N Tanigawa; K Shiraishi
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (8) 551 - 557 0369-4577 1998/08 [Refereed]
     
    A series of block copolymers, i.e., PHEMA-b-PDMS, PDMM-b-PDMS and PMDID-b-PDMS, were obtained from polymerization of 2-hydroxyethyl methacrylate (HEMA), 2,2-dimethyl-1,3-dioxolan-4-ylmethyl methacrylate (DMM) and 5-(5-hydroxymethyl-2,2-dimethyl-1,3-dioxolan-4-yl)-2,2-dimethyl-1,3-dioxolan-4-ylmethyl methacrylate (MDID) initiated with polydimethylsiloxane type of ate-initiator (VPS). Conversion of PDMM segment in PDMM-b-PDMS and PMDID segment in PMDID-b-PDMS into poly(2,3-dihydroxypropyl methacrylate) (PDHPMA) and poly(2,3,4,5,6-pentahydroxyhexyl methacrylate) (PMDul) segments by hydrolysis with a dilute hydrochloric acid resulted in PDHPMA-b-PDMS and PMDul-b-PDMS, respectively. From XPS measurements of freeze dried PHEMA-b-PDMS, PDHPMA-b-PDMS and PMDul-b-PDMS samples, the hydrophilic segment was found to migrate to the surface of copolymer films in water. The contact angle of water of copolymer films decreased with increasing the amount of the hydrophilic segment. The copolymer with a hydrophilic surface suppressed the adsorption of albumin (Alb) and gamma-globulin (Glo) as well as platelets, less than the polydimethylsiloxane as control.
  • Kazuo Sugiyama; Kazuya Kato; Masakazu Kido; Kohei Shiraishi; Koji Ohga; Kiyotaka Okada; Osamu Matsuo
    Macromolecular Chemistry and Physics Wiley-VCH Verlag 199 (6) 1201 - 1208 1022-1352 1998 [Refereed]
     
    Surface modified poly(ethylene terephthalate) (PET) films with 2-(methacryloyloxy)ethylphosphorylcholine (MPC) and 2-(glucosyloxy)ethyl methacrylate (GEMA) moieties, PMPC-g-PET and PGEMA-g-PET, were prepared by graft copolymerization using an Ar plasma-post polymerization technique. The degrees of polymerization of the grafts PMPC and PGEMA were P̄n ≈ 30 and P̄n ≈ 40, respectively. The contact angle of the modified PET film decreased from θ = 68° (the original PET film) to θ = 26° for PMPC-g-PET and to θ = 43° for PGEMA-g-PET. The modified PET films adsorb less serum proteins than the original PET film. Egg yolk lecithin did not adsorb on PGEMA-g-PET but adsorbed on PMPC-g-PET. PMPC-g-PET showed activity for the inhibition of fibrin formation and no adhesion of mouse fibroblasts (L-929).
  • K Sugiyama; K Isobe; K Shiraishi
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (11) 816 - 820 0369-4577 1997/11 [Refereed]
     
    Polyetherurethaneureas (PEUU-Si) including the tetramethyldisiloxane moiety in main chain were obtained from a typical two step polyaddition of polytetrahydrofuran (PTHF) TO 4,4'-methlenebis(phenyliscyanate) (MPI) in the presence of 1,3-bis(3-hydroxypropyl)-1,1,3,3-tetramethyldisiloxane (TMDS), using ethylenediamine (EDA) as a chain extension reagent. Polyaddition with a molar ratio of 1:2:1 for PTHF+TMDS:MPI:EDA gave the PEUU-Si in the mixed solvent of dimethyl sulfoxide and isobutyl methyl ketone, where the molar ratio of TMDS was changed from 0.2 to 1.0. From the XPS spectra and the contact angle measurements, it was found that the TMDS moiety was located on the surface of the PEUU-Si film in air and hydrophobicity was increased with increasing the molar ratio of the TMDS moiety. It was also found that PEUU-Si adsorbed bovine serum albumin. The tensile modulus of PEUU-Si showed E=66-120 MPa.
  • K Sugiyama; S Mitsuno; K Shiraishi
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY JOHN WILEY & SONS INC 35 (16) 3349 - 3357 0887-624X 1997/11 [Refereed]
     
    A series of microspheres composed of methyl methacrylate (MMA) and N(2-hydroxypropyl) methacrylamide (HPMA), and/or 2-(methacryloyloxy)ethyl phosphorylcholine (MPC), i.e., binary copolymer microspheres [poly(HPMA-co-MMA)(KPS) and poly(HPMA-co-MMA)(ABIP)] and ternary ones [poly(HPMA/MPC-co-MMA)(KPS) and poly(HPMA/MPC-co-MMA)(ABIP)], were prepared by emulsifier-free emulsion copolymerization using potassium peroxodisulfate (KPS) or 2,2'-azobis [2-(imidazolin-2-yl)propane]dihydrochloride (ABIP) as initiators. The decrease in zeta-potential of the polymer microspheres is caused by the addition of the HPMA and/or MPC moieties. Equilibrium water content of poly(HPMA-co-MMA)(ABIP) showed a remarkable swelling change with a change in response to temperature: the hydrated conformation at 28 degrees C and the dehydrated one at above 40 degrees C. The adsorption of protein on the polymer microspheres also changed in response to change in temperature. The ternary polymer microspheres effectively suppressed the adsorption both of Alb and Glo, less than binary ones. A series of polymer microspheres are expected to apply as a novel drug carrier with both thermosensitive and nonthrombogenic functions. (C) 1997 John Wiley & Sons, Inc.
  • K Sugiyama; S Akita; Y Tomoi; K Hanaki; K Shiraishi; K Ueda
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (2) 139 - 146 0369-4577 1997/02 
    Polyetherurethaneureas (PEUUs) including methyl- or fluoro substituted biphenyldiyls(BP, nMBP, nFBP) in main chain were obtained from a typical two step addition polymerization of polytetrahydrofuran #1000(PTHF) to 4,4'-methylene bis(phenyl isocyanate) (MPI) in the presence of the substituted biphenyldiols, using ethylenediamine (EDA) as a chain extension reagent. Biphenyldiols used were 4,4'-biphenyldiol (BP), 3,3'-dimethyl-4,4'-biphenyldiol (2MBP), 3,3', 5,5'-tetramethyl-4,4'-biphenyldiol(4MBP), 3,3'-difluoro-4,4'-biphenyldiol (2MBP), 3,3', 5,5'-tetrafluoro-4,4'-biphenyldiol (4FBP), and 2,2', 3,3', 5,5', 6,6'-octafluoro-4,4'-biphenyldiol (8FBP). Polyaddtion with a molar ratio of 0.5 : 0.5 : 2 : 1 for the biphenyidiol : PTHF : MPI : EDA in the mixed solvent of DMSO and IBMK(1 : 1) gave the PEUUs such as PEUU-BP, PEUU-nMBP, PEUU-nFBP. Parent polyetherurethaneurea (PEUU) was also prepared with a molar ratio of 1 : 2 : 1 for PTHF : MPI : EDA. XPS spectra of the PEUUs indicated that the hydrophobic segments containing the substituted biphenyldiyl moieties are located on the surface of the PEUUs film in air. The measurements of contact angle to water confirmed that the introduction of methyl groups or fluorine atoms into biphenyl ring results in higher hydrophobicity of PEUUs film surface. The tensile modulus(E) showed the values of E=109.1 MPa and E=129.3 MPa for PEUU-4MBP and PEUU-4FBP, respectively. It was also found that PEUU-nMBP and PEUU-nFBP, adsorb both bovine serum albumin and human serum gamma-globulin with a single layer. In cell culture test, the PEUUs films showed the adhesiveness of mouse fibroblast(L-929). Because of their mechanical and biocompatible properties, PEUU-nMBP and PEUU-nFBP are expected to be useful materials as an artificial blood vessel.
  • K Shiraishi; T Ohnishi; K Sugiyama; K Okada; O Matsuo
    CHEMISTRY LETTERS CHEMICAL SOC JAPAN (9) 863 - 864 0366-7022 1997 [Refereed]
     
    The poly(methyl methacrylate) microspheres [poly(SerMA-co-MMA)], modified their surface with novel zwitterionic O-methacryrloyl-L-serine (SerMA) were prepared bl! emulsifier-free emulsion copolymerization of SerMA and MMA. Poly(SerMA-co-MMA) suppressed the adsorption of serum protein and the activation of platelet.
  • K Sugiyama; S Mitsuno; Y Yasufuku; K Shiraishi
    CHEMISTRY LETTERS CHEMICAL SOC JAPAN (3) 219 - 220 0366-7022 1997 
    The aqueous solution of copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and alkyl methacrylate (RMA, alkyl :methyl or butyl) with molar ratio of RMA more than 0.08 was found to exhibit the LCST though no phase transition occurred for the case of the homopolymer of HPMA.
  • Kazuo Sugiyama; Mitsuteru Okabe; Kohei Shiraishi; Yoshihito Kadoma
    Kobunshi Ronbunshu Society of Polymer Science 53 (8) 496 - 505 0386-2186 1996 
    Glycerol-terminated macromonomers Mac(MPC/BMA)-I∼III (Mn= 1.9×104, 7×103, 4×103) were obtained from radical copolymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) and butyl methacrylate (BMA) in the presence of thioglycerol with different concentrations. Polyaddition was conducted for the syntheses of a series of copolyetherurethaneureas [PEU-g-P(MPC/BMA)-I∼III] grafted on the copolymer of MPC and BMA with the molar ratio of 0.1 : 0.9 : 2 : 1 for Mac(MPC/BMA)-I∼III : polytetrahydrofuran (PTHF) : 4,4′-diphenylmethane diisocyanate (DPMDI) : ethylene diamine. Parent polyetherurethaneurea (PEU) and poly(butyl methacrylate) graft copolyetherurethaneurea (PEU-g-PBMA) were also prepared. XPS spectra indicated that the MPC unit is located on the surface of PEU-g-P(MPC/BMA)-I∼III. The measurements of equilibrium water content and contact angle to water confirmed that the higher molecular weight of side chains results in higher hydrophilicity of PEU-g-P(MPC/ BMA)-I∼III surface. The tensile modulus showed values of E=13.3∼38.4 MPa for PEU-g-P(MPC/BMA)-I∼III films. It was also found that PEU-g-P(MPC/BMA)-I∼III suppressed the adsorption of both bovine serum albumin and human serum r-globulin less than PEU and PEU-g-PBMA. In cell culture test, PEU-g-P(MPC/BMA)-I, III films showed no adhesiveness of mouse fibroblast (L-929). Because of their mechanical and biocompatible properties, PEU-g-P(MPC/BMA)-I∼III are expected to be useful as novel biomaterials.
  • K Sugiyama; M Okabe; K Shiraishi; Y Kadoma
    KOBUNSHI RONBUNSHU SOC POLYMER SCIENCE JAPAN 53 (8) 496 - 505 0386-2186 1996 
    Glycerol-terminated macromonomers Mac(MPC/BMA)-I similar to III (M(n)=1.9X10(4), 7X10(3), 4X10(3)) were obtained from radical copolymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) and butyl methacrylate (BMA) in the presence of thioglycerol with different concentrations. Polyaddition was conducted for the syntheses of a series of copolyetherurethaneureas [PEU-g-P(MPC/BMA)-I similar to III] grafted on the copolymer of MPC and BMA with the molar ratio of 0.1:0.9:2:1 for Mac(MPC/BMA)-I similar to III:polytetrahydrofuran (PTHF):4,4'-diphenylmethane diisocyanate (DPMDI):ethylene diamine. Parent polyetherurethaneurea (PEU) and poly(butyl methacrylate) graft copolyetherurethaneurea (PEU-g-PBMA) were also prepared. XPS spectra indicated that the MPC unit is located on the surface of PEU-g-P(MPC/BMA)-I similar to III. The measurements of equilibrium water content and contact angle to water confirmed that the higher molecular weight of side chains results in higher hydrophilicity of PEU-g-P(MPC/BMA)-I similar to III surface. The tensile modulus showed values of E=13.3 similar to 38.4 MPa for PEU-g-P(MPC/BMA)-I similar to III films. It was also found that PEU-g-P(MPC/BMA)-I similar to III suppressed the adsorption of both bovine serum albumin and human serum gamma-globulin less than PEU and PEU-g-PBMA. In cell culture test, PEU-g-P(MPC/BMA)-I, III films showed no adhesiveness of mouse fibroblast (L-929). Because of their mechanical and biocompatible properties, PEU-g-P(MPC/BMA)-I similar to III are expected to be useful as novel biomaterials.
  • K SHIRAISHI; T NAKAMURA; K SUGIYAMA
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN 1995 (11) 925 - 928 0369-4577 1995/11 
    3-O-[(E)-3-(Hexadecyloxycarbonyl)acryloyl]-alpha-D-glucopyranose (gHDF) and 3-O-[(E)-3-(hexadecyloxycarbonyl) acryloyl]-1,2:5,6-di-O-isopropylidene-alpha-D-glucofurano (digHDF) were synthesized as a glycolipid analog having polymerizable group. gHDF and digHDF were polymerized in the presence of alpha,alpha'-azobisisobutyronitrile to give polymers [poly(gHDF) and poly(digHDF)]. The molecular assembly of gHDF and digHDF was examined by the solubilization of sodium 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescence probe. From the relationship between the concentration of glycolipid analogs and the fluorescence intensity, a sharp increase in fluorescence intensity for all of analogs was observed in chloroform at 10.0 x 10(-3), 0.8 x 10(-3), 2.5 x 10(-3), and 1.5 x 10(-3) mol L-l for gHDF, poly(gHDF), digHDF, and poly(digHDF), respectively. Therefore, glycolipid analogs obtained were found to form a molecular assembly in chloroform.
  • K Sugiyama; S Akita; K Shiraishi
    FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS FUDAN UNIVERSITY 137 - 138 1995 [Refereed]
  • K Shiraishi; T Onishi; K Sugiyama
    FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS FUDAN UNIVERSITY 135 - 136 1995 [Refereed]
  • Kohei Shiraishi; Tsutomu Nakamura; Kazuo Sugiyama
    Nippon Kagaku Kaishi 1995 (11) 925 - 928 0369-4577 1995 
    3-O-[(E)-3-(Hexadecyloxycarbonyl)acryloyl]-αpha -D-glucopyranose (gHDF) and 3-0-[(E)-3-(hexadecyloxycarbonyl)acryloyl] —1,2: 5,6-di-O-isopropylidene-αpha -D-glucofuranose (digHDF) were synthesized as a glycolipid analog having polymerizable group. gHDF and digHDF were polymerized in the presence of αpha ,αpha ′.-azobisisobutyronitrile to give polymers [poly (gHDF) and poly (digHDF)]. The molecular assembly of gHDF and digHDF was examined by the solubilization of sodium 8-anilino-l-naphthalenesulfonate (ANS) as a fluorescence probe. From the relationship between the concentration of glycolipid analogs and the fluorescence intensity, a sharp increase in fluorescence intensity for all of analogs was observed in chloroform at 10.0 x 10-3, 0.8 x 10-3, 2.5 x 10-3, and 1.5 x 10-3 mol-L-1 for gHDF, poly (gHDF), digHDF, and poly (digHDF), respectively. Therefore, glycolipid analogs obtained were found to form a molecular assembly in chloroform. © 1995, The Chemical Society of Japan. All rights reserved.
  • T OTSU; K SHIRAISHI; A MATSUMOTO
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY JOHN WILEY & SONS INC 31 (10) 2523 - 2529 0887-624X 1993/09 [Refereed]
     
    Radical polymerization of fumarates bearing different alkyl ester groups (DRF) on the same molecules was investigated. In bulk polymerization of DRF at 60-degrees-C initiated with 2,2'-azobis (isobutyronitrile), it was confirmed that the polymerization reactivity depended on the structures of both alkyl ester groups. The introduction of bulky alkyl groups increased the polymerization rate and molecular weight of the polymer because of retardation of bimolecular termination rates. The effect of the ester substituents on the termination was examined by electron spin resonance spectroscopy. The copolymerization reactivities of DRF with styrene were also investigated. (C) 1993 John Wiley & Sons, Inc.
  • T OTSU; K SHIRAISHI; A MATSUMOTO
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY JOHN WILEY & SONS INC 31 (4) 885 - 890 0887-624X 1993/03 [Refereed]
     
    Di-tert-butyl maleate (DtBM) did not polymerize with 2,2'-azobis (isobutyronitrile) as a radical initiator, but DtBM easily homopolymerized via a monomer-isomerization radical polymerization mechanism to give a high molecular weight polymer when morpholine was added into the polymerization system as an isomerization catalyst. The feature of the monomer-isomerization polymerization-of DtBM was investigated in detail. The polymer obtained was confirmed to consist of a poly (tert-butoxycarbonylmethylene) structure similar to that from di-tert-butyl fumarate. Subsequent pyrolysis of the resulting polymer at 180-degrees-C is a useful route to synthesis of a high molecular weight poly(fumaric acid).
  • Synthesis and properties of terminal-terminal type of smectic liquid crystals
    SHIRAISHI Kohei
    Chemistry Express 8 (3) 189 - 192 1993 [Refereed]
  • K SUGIYAMA; K SHIRAISHI; K KATO
    POLYMER JOURNAL SOC POLYMER SCIENCE JAPAN 25 (1) 103 - 108 0032-3896 1993
  • K SUGIYAMA; K SHIRAISHI; K OHGA; H SHIRAHAMA; H TAMAI; K KIKUKAWA; H YASUDA
    POLYMER JOURNAL SOC POLYMER SCIENCE JAPAN 25 (5) 521 - 527 0032-3896 1993 
    Microsphere, Poly(MAHP-co-MMA), was prepared by the emulsifier free emulsion copolymerization of 2-[2-(methacryloyloxy)ethyldimethylammonio]ethyl-6-[4-(4-methoxyphenylazo)phenoxy]hexyloxyphosphate (MAHP) and methyl methacrylate (MMA). The diameter of poly (MAHP-co-MMA) estimated from TEM varied from 234 to 402 nm. depending on the mol-% of MAHP to MMA in feed from f = 0 to f = 0.5. From XPS measurements of poly (MAHP-co-MMA) the polar head groups of MAHP were found to be located on the surface of particles. The adsorption of bovine serum albumin (BSA) onto poly(methyl methacrylate) [poly(MMA)] and poly(MAHP-co-MMA) microspheres were examined at pH 5.6. The introduction of phosphatidylcholine analog into poly(MMA) results in a drastic decrease in BSA adsorption. A controlled decrease in BSA adsorption was also observed in poly(MAHP-co-MMA) with cis-azobenzene moieties caused by UV irradiation.
  • K SUGIYAMA; K KATO; K SHIRAISHI
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN CHEMICAL SOC JAPAN 65 (8) 2259 - 2263 0009-2673 1992/08 
    Novel terminal-terminal types of liquid crystalline bis[urethane]s 3, prepared from (S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 2 and various diisocyanates, were studied by differential scanning calorimetry (DSC) and polarized optical microscopy (POM). Bis[urethane]s 3c obtained from 1,3-diisocyanatobenzene and 3d from 2,4-diisocyanatotoluene exhibit a smectic state, while bis[urethane]s 3a obtained from 1,6-diisocyanatohexane, 3b from bis(4-isocyanatophenyl)methane and 3e from 2,6-diisocyanatotoluene show no mesophases. It was found from IR spectroscopic results that inter- and intramolecular hydrogen bondings due to N-H and C=O of urethane bonds play an important role in the formation of mesophase. The dual-frequency drive, based on light scattering driven by the application of an a.c. current at different frequencies, was studied in the smectic A state of 3c and 3d: tau(r) at 0.1 Hz are 0.84 and 0.25 s, tau(r) at 1 kHz are 9.75 and 8.95 s for 3c and 3d, respectively.
  • T OTSU; A MATSUMOTO; K SHIRAISHI; N AMAYA; Y KOINUMA
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY JOHN WILEY & SONS INC 30 (8) 1559 - 1565 0887-624X 1992/07 
    Radical copolymerization of dialkyl fumarates (DRF) with various vinyl monomers was carried out in benzene at 60-degrees-C. The monomer reactivity ratios, r1 and r2, were determined from the comonomer-copolymer composition curves. The relative reactivity of DRFs with various ester substituents toward a polystyryl radical was revealed to depend on both steric and polar effects of the ester groups. It has also been clarified that alpha-substituents of the polymer radical have a significant role in addition of DRF, from the comparison of the monomer reactivity ratios determined in copolymerizations with monosubstituted and 1,1-disubstituted ethylenes. The absolute cross-propagation rate constants were also evaluated and discussed.
  • Synthesis and Polymerization of 12-meth-acryloyloxydodecyl 2-(dimethyl-hexadecylammonio)ethylphosphate
    SHIRAISHI Kohei
    Chemistry Express 7 (4) 261 - 264 1992 [Refereed]
  • T OTSU; M YOSHIOKA; A MATSUMOTO; K SHIRAISHI
    POLYMER BULLETIN SPRINGER VERLAG 26 (2) 159 - 164 0170-0839 1991/07 
    High molecular weight poly(dialkyl fumarate)s bearing n-alkyl esters such as an octadecyl group were prepared efficiently from poly(di-tert-butyl fumarate) by the one-pot synthesis, i.e. both elimination of isobutene from the tert-butyl ester and the subsequent esterification were performed in a corresponding alcohol at 170-180-degrees-C in a one-pot without isolating poly(fumaric acid). The resulting polymers were characterized by IR and NMR spectroscopies and GPC. The polymers with various contents of an octadecyl group in the side chain were also obtained similarly from the copolymer of di-tert-butyl fumarate with diisopropyl fumarate.
  • K SUGIYAMA; K SHIRAISHI
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN CHEMICAL SOC JAPAN 64 (5) 1715 - 1717 0009-2673 1991/05 
    2-Methylenesuccinates (Itaconates) (2) having two 4-methoxyazobenzene moieties as paired mesogenic groups were prepared. The monomer 2 was radically homo-polymerized and copolymerized with non-mesogenic monomer, dioctadecyl itaconate (DOI). Paired mesogenic homopolyitaconate exhibits a nematic phase and the copolymers of the monomer 2 with DOI also show a mesomorphic phase up to 25 mol% of DOI content. The enhancement of mesophase formation by paired mesogens is discussed.
  • K SUGIYAMA; K KATO; K SHIRAISHI
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN CHEMICAL SOC JAPAN 64 (5) 1652 - 1657 0009-2673 1991/05 
    Four kinds of ferroelectric liquid crystals (FLCs) possessing OH groups, ((S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 4a, (S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)2-hydroxybenzylideneamino]cinnamate 4b, (S)-2-hydroxypropyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 4c, and (S)-2-hydroxypropyl 4-[4-(11-hydroxyundecyloxy)-2-hydroxybenzylideneamino]cinnamate 4d) were prepared in order to understand how the introduction of OH groups in FLCs affect the phase behavior and electrooptical effects. Alpha,omega-Diols 4c and 4d show a higher transition temperature of the smectic A (S(A)) phase than do omega-hydroxyl compounds 4a and 4b. Comparing 4b and 4d both of which have an OH group at the omicron-position of the benzylidene group with 4a and 4c, the former samples show a higher S(A)-isotropization (I) transition temperature than do the latter ones. It is suggested from IR spectroscopic results that inter- and intramolecular hydrogen bonding contribute to the stabilization of the S(A) phase. The order of the helical pitch in the chiral smectic C (S(c)*) phase was found to be 4b > 4a > 4d > 4c within 1.8-6.6-mu-m. From an electrooptical effect due to a deformation of the helical structure in the S(c) phase, the rise time of the surface director (tau-sr) and the rise time of the bulk director (tau-br) were measured to be as follows: tau-sr are 850, 1500, 30, and 70-mu-s, and tau-br are 12, 100, 0.17, and 0.35 ms for 4a, 4b, 4c, and 4d, respectively. It was found that the intramolecular hydrogen bonding results in a slower optical response time, whereas intermolecular hydrogen bonding results in a faster one.
  • K SUGIYAMA; Y MORIKAWA; K SHIRAISHI
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (3) 230 - 234 0369-4577 1991/03 [Refereed]
     
    Three kinds of alpha-olefinic liquid crystals, 4-[4-[(S)-2-methylbutoxycarbonyl]phenoxycarbonyl]phenyl 10-undecenoate 1, 4-[4-[(S)-2-methylbutoxycarbonyl)]phenyl] phenyl 10-undecenoate 2, and (S)-2-methylbutyl 4-[N-4-(10-undecenoyloxy)benzylideneamino] cinnamate 3 were synthesized as reactive monomers for ferroelectric liquid crystalline comb-like polysiloxanes. 1 shows a smectic A(S(A)) state, while 2 and 3 show both S(A) and chiral smectic C (S(C)(*)) state. From the electrooptical measurements, the response times for dual frequency drive in S(A) state were found to be 30, 310, and 30 ms at 0.1 Hz : 2. 12, 7, and 2.67 s at 1 kHz for 1, 2 and 3, respectively. The response time for SSFLC in S(C)(*) phase was also found to be 460 and 105-mu-s for 2 and 3, respectively.
  • Kazuo Sugiyama; Yoshifumi Morikawa; Kohei Shiraishi
    Nippon Kagaku Kaishi 1991 (3) 230 - 234 0369-4577 1991 [Refereed]
     
    Three kinds of αpha -olefinic liquid crystals, 4-[4-[(5)-2-methylbutoxycarbonyl]phenoxycar-bonyl]phenyl 10-undecenoate 1, 4-[4-[(S)-2-methylbutoxycarbonyl]phenyl]plienyl 10-undecenoate 2, and (S)-2-methylbutyl 4-[N-4-(10-undecenoyloxy)benzylideneamino]cinnamate 3 were synthesized as reactive monomers for ferroelectric liquid crystalline comb-like polysiloxa-nes. 1 shows a smectic A(SA) state, while 2 and 3 show both SA and chiral smectic C (Sc∗) state. From the electrooptical measurements, the response times for dual frequency drive in SA∗ state were found to be 30, 310, and 30 ms at 0.1 Hz: 2.12, 7, and 2. 67 s at 1 kHz for 1, 2 and 3, respectively. The response time for SSFLC in Sc∗ phase was also found to be 460 and 105 μ s for 2 and 3, respectively. © 1991, The Chemical Society of Japan. All rights reserved.
  • K SUGIYAMA; K SHIRAISHI; T IHARA; M KIBOKU
    POLYMER JOURNAL SOC POLYMER SCIENCE JAPAN 23 (10) 1287 - 1290 0032-3896 1991 [Refereed]
  • B YAMADA; E YOSHIKAWA; K SHIRAISHI; H MIURA; T OTSU
    POLYMER ELSEVIER SCI LTD 32 (10) 1892 - 1896 0032-3861 1991 
    During polymerization of diisopropyl fumarate (DiPF), the propagating poly(DiPF) radical was scavenged quantitatively by a stable free radical, because of its long-lived nature and high steady-state concentration. By addition of a known amount of 1,3,5-triphenylverdazyl to the polymerization mixture, the concentration of poly(DiPF) radical was determined to be 10(-5)-10(-4) mol dm-3, which allowed the evaluation of the propagation rate constant (k(p)) from the overall rate of polymerization: k(p) = 0.31 +/- 0.07 dm3 mol-1 S-1 at 30-degrees-C. The lifetime of the polymer radical was too long for a rotating sector experiment. The rate constant for bimolecular termination of the radical (k(t)) was calculated from the decay of poly(DiPF) radical followed by the scavenger method: k(t) = 0.84 dm3 mol-1 S-1. These rate constants, which are exceptionally small, are accounted for by the steric factor of the ester alkyl group accumulated along the polymer chain and extremely slow diffusion of the polymer radical. Because the termination rate determined is too slow to interpret the molecular weight of the polymer formed, primary radical termination is to be considered as an additional termination process in the actual polymerization.
  • K SHIRAISHI; K SUGIYAMA
    CHEMISTRY LETTERS CHEMICAL SOC JAPAN (9) 1697 - 1700 0366-7022 1990/09
  • K SUGIYAMA; K SHIRAISHI
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (8) 876 - 879 0369-4577 1990/08 [Refereed]
  • LIQUID-CRYSTALLINE POLYMERS PREPARED BY POLYADDITION OF DITHIOLS TO MESOGENIC BISACRYLATES OR BISMETHACRYLATES
    K. SUGIYAMA; K. SHIRAISHI
    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY 28 (7) 1983 - 1988 1990/06
  • TERMINAL-TERMINAL TYPES OF LIQUID-CRYSTALS .1. SYNTHESIS AND ELECTROOPTICAL PROPERTIES OF A FERROELECTRIC LIQUID-CRYSTAL
    K SHIRAISHI; K KATO; K SUGIYAMA
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 63 (6) 1848 - 1850 1990/06
  • K SHIRAISHI; K KATO; K SUGIYAMA
    CHEMISTRY LETTERS CHEMICAL SOC JAPAN (6) 971 - 974 0366-7022 1990/06 [Refereed]
  • Kazuo Sugiyama; Kohei Shiraishi
    NIPPON KAGAKU KAISHI 1990 (8) 876 - 879 0369-4577 1990 [Refereed]
     
    2-s-Butyl-4, 6-dinitrophenol (BDNP) was chemically modified to polymerizable amphiphile, [10-(2-s-butyl-4,6-dinitrophenoxycarbonyl)deeyl] [2-(methaeryloyloxy)ethyl]dimethylammo-nium bromide via 2-s-butyl-4,6-dinitrophenyl 11-bromoundecanoate 1. 2 was converted to a,oligomer 3 with ca. 6500 of molecular weight by a radical polymerization. Krafft point of 2 was found to be 32. 3°C. CMC values of 2 and 3 were found to be 2.7xl0-4 and 4.0xl0-4 mpl//, respectively by the measurements of conductivity of their aqueous solution. 1, 2, and 3 exhibit antibacterial activity against Staphylococcus. © 1990, The Chemical Society of Japan. All rights reserved.
  • K SUGIYAMA; K SHIRAISHI
    MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS HUTHIG & WEPF VERLAG 190 (10) 2381 - 2390 0025-116X 1989/10 [Refereed]
  • K SHIRAISHI; K SUGIYAMA
    MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS HUTHIG & WEPF VERLAG 190 (9) 2235 - 2243 0025-116X 1989/09 [Refereed]
  • Shiraishi Kohei; Sugiyama Kazuo; Otsu Takayuki
    Bull. Chem. Soc. Jpn. The Chemical Society of Japan 62 (12) 4083 - 4085 0009-2673 1989 
    Nematic polyitaconates having 4-methoxyazobenzene moieties as mesogen in the side chain were prepared. Thermal properties, polymer composition, and the photoinduced configuration change have been examined to suggest a possible use of the polymers for photosensitive liquid-crystalline materials.
  • K SHIRAISHI; A SAKAMOTO; K SUGIYAMA
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (6) 946 - 951 0369-4577 1988/06 [Refereed]
  • K SHIRAISHI; K SUGIYAMA; A SAKAMOTO; T OTSU
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN CHEMICAL SOC JAPAN 61 (3) 783 - 786 0009-2673 1988/03 [Refereed]
  • K SHIRAISHI; K SUGIYAMA
    JOURNAL OF MACROMOLECULAR SCIENCE-CHEMISTRY MARCEL DEKKER INC A25 (8) 1015 - 1025 0022-233X 1988 [Refereed]
  • K SUGIYAMA; A SAKAMOTO; Y MURATA; K SHIRAISHI; T OTSU
    NIPPON KAGAKU KAISHI CHEMICAL SOC JAPAN (6) 1064 - 1070 0369-4577 1987/06 [Refereed]
  • T OTSU; K SHIRAISHI; A MATSUMOTO; T YASUHARA; N AMAYA; S MORI
    POLYMER COMMUNICATIONS BUTTERWORTH-HEINEMANN LTD 26 (12) 367 - 368 0263-6476 1985
  • MONOMER-ISOMERIZATION RADICAL POLYMERIZATION OF DI-TERT-BUTYL MALEATE TO HIGH MOLECULAR-WEIGHT POLY(DI-TERT-BUTYL FUMARATE)
    T. OTSU; K. SHIRAISHI
    MACROMOLECULES 18 (9) 1795 - 1796 1985
  • T OTSU; T YASUHARA; K SHIRAISHI; S MORI
    POLYMER BULLETIN SPRINGER VERLAG 12 (5) 449 - 456 0170-0839 1984 [Refereed]

Books etc

  • 大庭敏之 (p.230-p.240)シーエムシー出版 2020/10 9784781315607 v, 284p
  • 刺激応答性高分子ハンドブック
    白石 浩平他 (第6節 ポリエチレングリコール(PEG)修飾マイクロアレイ基板を用いるパルスレーザー照射による遺伝子導入)エヌ・ティー・エス 2018/12
  • セルロースナノファイバーの均一分散と複合化
    白石 浩平他 (第9節 メカニカル解繊セルロースナノファイバーの湿式プロセス複合化による天然ゴム(NR)の物性改善)サイエンス＆テクノロジー 2018/07
  • 化学と工業 第58巻，第3号
    白石浩平; 山本和彦 (生体システムの利用を目指すアミノ酸修飾高分子を用いた新規な生医学材料の調製と応用 pp237-238)日本化学会 2005/06
  • The 2nd International School of advanced plasma technology, Villa Monastero, Varenna, Italy, Biological characterization of nano carbon coating or/and biocompatible polymer grafting commodity materials for implantable medical devices by low temperature pl
    白石 浩平; 杉山 一男 (Joint work)2004/10
  • ナノバイオエンジニアリングマテリアル第IV編バイオナノマトリックス第7章アミノ酸ユニットを担持した機能性ポリマー
    白石 浩平; 杉山 一男 (Joint work)フロンティア出版 2004/03 490241001X 
    アミノ酸ユニットを担持もつ新規なポリマーのバイオマテリアルへの応用と機能について解説した．

Conference Activities & Talks

  • 新規バインダーを用いる（フルオロ）ヒドロキシアパタイト固定化Ti 基板の調製と骨芽細胞の増殖活性
    秋田泰孝; 渡邉真理子; 倉谷慧; 相良宗作; 山本高久; 白石浩平; 岡田清孝; 梶博史
    第71回高分子討論会  2022/09
  • ポリスチレンの土壌真菌による表面性状の変化
    岸本崇勢; 大西芽衣; 小川智弘; 白石浩平
    第71回高分子討論会  2022/09
  • PET 配合茶葉バイオコークスの構造と熱的性質
    岸本崇勢; 鵜飼友哉; 笹井美佳; 白石浩平; 中谷都志美; 大下浄治; 井田民男
    第71回高分子討論会  2022/09
  • 低温プラズマ処理による含酸素官能基導入フッ素樹脂と平滑銅箔の 直接接合体の調製
    平尾成隆; 松本和也; 崔源煥; 久武信太郎; 白石浩平; 與倉三好; 永島正嗣
    第71回高分子討論会  2022/09
  • 焼結法によるヒドロキシアパタイトのチタン表面固定化と骨芽細胞分化と骨伝導性の向上
    秋田泰孝; 相良宗作; 山本高久; 白石 浩平; 岡田清孝; 梶博史
    2021年日本化学会中国四国支部大会 高知大会  2021/11
  • 平滑銅箔と低温プラズマ処理によりOH基，COOH基導入したフッ素樹脂の直接接合体の調製
    平尾成隆; 松本和也; 崔源煥; 久武信太郎; 白石浩平; 與倉三好; 永島正嗣
    高分子学会 第70回年次大会  2021/05
  • 合成高分子含有バイオコークスの内部構造と分子運動性変化
    白石 浩平
    令和02年度近畿大学バイオコークス研究所・外部評価委員会基調講演  2021/03
  • 医学教育の中の化学教育におけるオンライン教育の実践と成果
    岡田 清孝; 武知 薫子; 白石 浩平; 松村 治雄
    第101回日本化学会年次大会  2021/03
  • 超平滑銅箔／低温プラズマ表面処理フッ素樹脂の接着剤レス接合体の調製
    松本和也; 平尾成隆; 崔源煥; 久武信太朗; 白石浩平; 興倉三好; 永島正嗣
    第29回 ポリマー材料フォーラム  2020/11
  • L-リジンを持つアクリルアミドと各種共重合体調製及び共重合体構造が与える線溶活性への影響  [Not invited]
    白石浩平,沖勇斗,杉本奈美
    第69回高分子学会年次大会  2020/05
  • スター型ポリマ―が中間水形成と酵素活性に与える影響  [Not invited]
    白石浩平; 武部佑紀; 沖勇斗
    第69回高分子学会年次大会  2020/05
  • 新規濡れ性試験AILE法を用いたMPCポリマー被覆表面の膨潤挙動の解析  [Not invited]
    児島千恵; 片山里紗; 田中信行; 田中陽; 白石浩平; 松本章一
    第69回高分子学会年次大会  2020/05
  • 医学部初年度学生に対する新たな化学授業の成果―医療に関連づけた実習の導入
    岡田 清孝; 白石 浩平; 小川智弘; 武知 薫子; 松村 治雄; 梶 博史
    第100回日本化学会年次大会  2020/03
  • Preparation of Randomcopolymers with poly(acrylamide) having L-Lysine Moiety and 2-(Methacryloyloxy)ethyl Phosphorylcholine and its Effect on Fibrinoltic Activity  [Not invited]
    Yuto OKI; Kohei SHIRAISHI
    THE 16TH PACIFIC POLYMER CONFERENCE  2019/12
  • Preparation of Star-shaped L-lysine Acrylamide Polymer and Its Influence on Fibrinolytic Enzyme Reaction.  [Not invited]
    Yuki TAKEBE; Kohei SHIRAISHI; Yuto OKI
    THE 16TH PACIFIC POLYMER CONFERENCE  2019/12
  • L-リジンを側鎖に含むスター型ポリアクリルアミドの調製と線溶活性への影響  [Not invited]
    武部佑紀; 沖勇斗; 松本和也; 白石浩平
    日本化学会中四国支部大会徳島大会  2019/11
  • Evaluation of wettability and swelling behaviors of zwitterionic polymers using non-contact wettability evaluation system  [Not invited]
    Risa Katayama; Chie Kojima; Nobuyuki Tanaka; Yo Tanaa; Kohei Shiraishi; Akikazu, Matsumoto
    JSAMA-8  2019/11
  • 線溶因子結合能を持つ両性イオン構造アミノ酸を側鎖に含む抗血栓材料の開発  [Not invited]
    白石 浩平
    関西大学先端科学技術推進機構研究部門発表会(第59回)  2019/07
  • ドデシル基をもつMPCポリマー上での疎⽔性ドメイン形成とＲＡＷ細胞による特異な認識（D-1)  [Not invited]
    片山里紗; 白石浩平; 松本章一; 児島千恵
    第65回⾼分⼦研究発表会(神戸)  2019/07
  • ⾮接触濡れ性評価システムを⽤いた細胞膜模倣ポリマーコーティング表⾯の濡れ性評価  [Not invited]
    片山里紗; 児島千恵; 田中信行; 田中陽; 白石浩平; 松本章一
    第48回医⽤⾼分⼦シンポジウム (東京)  2019/07
  • 医学部学生のおける化学のモチベーションと成績の傾向
    岡田 清孝; 武知 薫子; 松村 治雄; 梶 博史; 白石 浩平
    第99回日本化学会年次大会  2019/03
  • MPC/メタクリル酸ドデシル共重合体をコーティングした各種基材の表面性状と水膨潤挙動  [Not invited]
    髙木優介; 武部佑紀; 白石浩平; 児島千恵; 松本章一
    第67回高分子討論会（北海道）  2018/09
  • リン脂質模倣ポリマーコーティングによる種々素材への細胞接着特性  [Not invited]
    片山里紗; 池田武蔵; 児島千恵; 白石浩平; 松本章一
    第64回高分子研究発表会 (神戸)  2018/07
  • Preparation of poly[(meth)acrylamide]having L-lysine Moiety and its Effect on Fibrinolytic Activity  [Not invited]
    SHIRAISHI Kohei
    ICBZM2017  2017/10
  • Adhesion Properties of MPC Copolymer to Substrates and Cells  [Not invited]
    Chie kojima; Musashi Ikeda; Akikazu Matsumoto; Kenji Kajiyama; Tohru Sakoda; Kohei SHIRAISHI
    IUMRS-ICAM2017  2017/08
  • Evaluation of Adhesive Properties and Cell Adhesion of MPC Polymer-Coated Substrates  [Not invited]
    Musashi Ikeda; Chie kojima; kenji Kajiyama; Tohru Sakoda; Kohei Shiraishi; Akikazu Matsumoto
    ICBS  2016/11
  • 新規親水性塗膜による金属表面への修飾と不凍性の評価  [Not invited]
    小坂 一貴; 今城 明典; 平井 克尚; 白石 浩平
    第31回中国四国支部高分子若手研究会「高分子の構造と機能」  2016/11
  • 生体応答を制御するバイオマテリアル及び新規レーザー蛍光顕微鏡装置による細胞診断/回収/融合と血液凝固診断  [Not invited]
    白石 浩平
    中国地域さんさんコンソ発ライフサイエンス新技術説明会  2016/11
  • 細胞マイクロアレイとレーザー照射システムを用いるハイスループット細胞診断・回収、及び高効率細胞融合の統合化装置の開発  [Not invited]
    白石 浩平; 近大産業理工; エステック株式会社; 鳥取大院医; 公財)ちゅうごく産業創造センター
    BioJapan  2016/10
  • リン脂質ポリマーをコートしたプラスチック基材の性能評価  [Not invited]
    池田武蔵; 児島千恵; 梶山健次; 迫田亨; 白石浩平; 松本章一
    第12回接着学会若手の会  2016/10
  • セルロースナノファイバー(CNF)等ナノ微粒子の新規な血液凝固評価試薬への応用  [Not invited]
    今城明典; 小坂一貴; 白石浩平; 岡野こずえ
    バイオマス関連開発事業推進会議バイオマス利用技術研究発表会  2016/09
  • 表面開始ATRP法でUCST型温度応答性アクリルアミド/アクリロニトリル共重合体を修飾したガラス基板の調製とヒト間葉系幹細胞(hiMSC)の温度刺激はく離  [Not invited]
    農宗 辰己; 今城 明典; 小坂 一貴; 伊藤 大時; 山田 康枝; 白石 浩平
    第65回高分子学会年次大会(神戸)  2016/05
  • Preparation Of A UCST Type Of Thermoresponsive Sulfobetaine Polymer Modified Poly(ethylene terephthalate)Films By Ar Plasma post Polymerization Method  [Not invited]
    Akinori IMAJO; Taiji ITO; SHIRAISHI Kohei
    The 10th Asian-Europeean International Conference on Plasma Surface Engineering 2015  2015/09
  • 表面開始RAFT重合法でUCST型温度応答性アクリルアミド/アクリロニトリル共重合体を修飾したガラス基板の調製とヒト間葉系幹細胞(hiMSC)の温度刺激はく離  [Not invited]
    農宗辰己; 今城明典; 山田康枝; 白石浩平
    第64回高分子討論会  2015/09
  • PEG固定化表面での血球系細胞とヒト人工染色体(HAC)ベクター含有微小核細胞の融合と細胞接着挙動  [Not invited]
    神崎有加; 今城明典; 山田康枝; 白石浩平
    第64回高分子討論会  2015/09
  • Preparation of UCST types of thermoresponsive polymer immobilized on a glass substrate by SI-RAFT polymerization method and thermal stimuli-exfoliation of hiMSC  [Not invited]
    農宗 辰己; 今城 明典; 山田 康枝; 白石 浩平
    第64回高分子学会年次大会  2015/05
  • Preparation of cell microarrays immobilized with protein binding sites and PEG segments on the glass spots and cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells  [Not invited]
    Akinori Imajo; Yuka Kanzaki; Kohei Shiraishi
    第64回高分子学会年次大会  2015/05
  • ポリカルボジイミドの接着機能を利用した天然素材（ポリ乳酸／天然ゴム／セルロースナノファイバー）の自動車部品並びに医用素材への応用  [Not invited]
    白石 浩平
    日本接着学会第2回関西支部広島講演会  2015/02
  • Preparation of cell microarray immobilized with various thermosensitive polymers on the glass spots and evaluation of cell exfoliation by thermal stimulation on their substrates  [Not invited]
    Tatsuki Nousou; Akinori Imajo; Yuka Kanzaki; Yasue Yamada; Kohei Shiraishi; Hirofumi Kawazumi
    第63回高分子討論会  2014/09
  • Highly efficient cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells by using cell microarrays immobilized with PEG on the glass spots  [Not invited]
    Akinori Imajo; Yuka Kanzaki; Yasue Yamada; Kohei Shiraishi
    第63回高分子討論会  2014/09
  • iPS細胞等の万能細胞の実用化を目指すポリマーバイオマテリアルの開発  [Not invited]
    白石 浩平
    広島県私立学校教育研修会  2014/08
  • 自動車部品の調製技術を展開したPLAの医用材料への応用－カーボンニュートラルから生分解性へ  [Not invited]
    白石 浩平
    文部科学省戦略研究プロジェクト地域連携による次世代自動車技術に関する研究  2014/07
  • Preparation of PNiPAAm immobilized polyacetylene films by plasma irradiation-post polymerization and cell attachment, proliferation, and thermal-stimuli exfoliation on their films  [Not invited]
    Tatsuki Nousou; Yasue Yamada; Kohei Shiraishi
    第63回高分子学会年次大会  2014/05
  • Preparation of PNiPAAm with various chain lengths immobilized glass spots on a microarray by SI-ATRP method and cell attachment and thermal stimuli-exfoliation of cells on the microarray  [Not invited]
    Akinori Imajo; Tatsuki Nousou; Yuka Kanzaki; Yasue Yamada; Kohei Shiraishi; Tatsuyuki Nakatani
    第63回高分子学会年次大会  2014/05
  • Preparation of PNiPAAm with various polymer chain lengths immobilized on a glass substrate by SI-ATRP method and cell attachment and thermal stimuli-exfoliation of mesenchymal stem cell on the substrate  [Not invited]
    Akinori Imajo; Manato Asai; Yasue Yamada; Kohei Shiraishi; Tatsuyuki Nakatani
    第62回高分子討論会  2013/09
  • Preparation of UCST type of poly(acrylamide-co-acrylonitrile) immobilized on a glass substrate by SI-ATRP method and heat-cell exfoliation on the substrate  [Not invited]
    Taiji Ito; Nousou Tatuki; Yasue Yamada; Kohei Shiraishi; Hirofumi Kawazumi; Tatsuyuki Nakatani
    第62回高分子討論会  2013/09
  • Improvement of Physicochemical Properties of Natural Rubber Reinforced with Mechanical nanofibrillated cellulose  [Not invited]
    Hirotaka Mita; Toru Yano; Kohei Shiraishi; Takashi Endo
    第62回高分子討論会  2013/09
  • メカニカル解繊セルロースナノファイバー配合による天然ゴムの物性改善  [Invited]
    白石 浩平
    日本ゴム協会関西支部講演  2013/08
  • 揮発性有機化合物の神経系細胞への効果  [Not invited]
    山田 康枝; 大谷 航平; 白石 浩平
    第40回 日本毒性学会学術年会  2013/06  千葉  第40回 日本毒性学会学術年会
  • Preparation of surface-modified substrates with UCST type of thermosensitive polymers and evaluation of cell exfoliation by thermal stimulation on their substrates  [Not invited]
    Taiji Ito; Tatsuki Nousou; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Hirofumi Kawazumi; Tatsuyuki Nakatani
    第62回高分子学会年次大会  2013/05
  • Highly efficient cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells by using cell microarrays  [Not invited]
    Akinori Imajo; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani
    第62回高分子学会年次大会  2013/05
  • iPS細胞等の有用細胞を分離回収する高性能細胞回収装置の開発  [Not invited]
    白石 浩平
    ちゅうごく産業創造センタービジネスマッチングフェア  2013/01
  • 細胞に番号を付ける！？新しい細胞操作のプラットフォーム創製を目指して  [Not invited]
    白石 浩平
    経済産業省インテレクチャルカフェ  2013/01
  • 揮発性化学物質の神経系細胞への効果  [Not invited]
    山田 康枝; 白石 浩平
    第85回 日本生化学会大会  2012/12  福岡  第85回 日本生化学会大会
  • ポリ乳酸 射出成形による自動車内装用モジュール部品の新規開発  [Not invited]
    白石 浩平
    第68回インターモールドテクニカルセミナー  2012/09  東京  第68回インターモールドテクニカルセミナー
  • Preparation of surface modified substrates with UCST or LCST types of thermosensitive polymer and evaluation of cell exfoliation on their surfaces by thermal stimulation or laser light irradiation  [Not invited]
    Manato Asai; Taiji Ito; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Hirofumi Kawazumi; Tatsuyuki Nakatani
    第61回高分子討論会  2012/09
  • Cell fusion of microcells with Human Artificial Chromosome(HAC) and white blood cells(U937) on microarray spots grafted PMPC with cell specific antibodies  [Not invited]
    Akinori Imajo; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani
    第61回高分子討論会  2012/09
  • セルロースナノファイバー分散バイオエラストマー複合材料の創製と有機揮発成分VOCs)の生体評価  [Not invited]
    白石 浩平
    戦略的研究プロジェクト平成23年度研究成果発表会・見学会  2012/09
  • Preparation of surface-modified substrates with UCST or LCST types of thermosensitive polymer and evaluation of cell attachment and exfoliation on their substrates  [Not invited]
    Taiji Ito; Manato Asai; Tatsuya Ishihara; Yasue Yamada; Kohei Shiraishi; Sugiyama Kazuo; Hirofumi Kawazumi; Tatsuyuki Nakatani
    第61回高分子学会年次大会  2012/05
  • Preparation of proteinA/G carrying poly(MPC) spotted microarray and evaluation of cell-specific attachment on the substrate  [Not invited]
    Akinori Imajo; Akihiro Tominaga; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Hirofumi Kawazumi; Tatsuyuki Nakatani
    第61回高分子学会年次大会  2012/05
  • 揮発性化学物質の神経系細胞への効果  [Not invited]
    山田 康枝; 白石 浩平; 独立行政法人酒類総合研究所; 理
    日本農芸化学会 2012年大会  2012/03  京都  日本農芸化学会 2012年大会
  • 抗凝血機能を付与した両性イオン構造セリン修飾ポリマーの調製と生体親和性の評価  [Not invited]
    白石 浩平; 杉山 一男
    2011/09  北海道  高分子学会
  • Preparation of Fluorinated Polyurethanes Having an Antifouling Ability  [Not invited]
    Tatsuya Kawakami; Kohei Siraisi; Kazuo Sugiyama; Naokatu Hisanaga
    第60回高分子討論会  2011/09
  • Preparation of thermoresponsive polymer-immobilized microarrays for cytoscreening and cell recovery  [Not invited]
    Akinori Imajo; Kazuo Sugiyama; Kohei Shiraishi; Yasue Yamada; Hirofumi Kawazumi; Tatsuyuki Nakatani; Keishi Okamoto; Yuki Nitta
    第60回高分子討論会  2011/09
  • Preparation of surface-modified substrates with anti-body carrying poly(MPC) and evaluation of cell specific attachment on their surfaces  [Not invited]
    Akihiro Tominaga; Akinori Imajo; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama
    第60回高分子討論会  2011/09
  • Development of system cell diagnosis and removal of unnecessary cells by using visible laser light irradiating to cell microarray  [Not invited]
    Tatyuya Ishihara; Ryota Kobayashi; Manato Asai; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiya; Hirofumi Kawazumi; Tatsuyuki Nakatani; Keishi Okamoto; Yuki Nitta
    第60回高分子討論会  2011/09
  • 自動車軽量化に応える環境調和型バイオプラスチックの可能性と活用のための新技術  [Invited]
    白石 浩平
    平成23年度第1回次世代自動車技術研究会  2011/08
  • Preparation and characterization of anti-freezing paint by using a polyion complex  [Not invited]
    Ryohei Shimoji; Kohei Shiraishi; Kazuo Sugiyama
    第60回高分子学会年次大会  2011/05
  • Preparation of cell microarray modified with poly(MPC) and/or thermosensitive polymer for cell diagnosis and acquisition of targeting cell by using visible light laser  [Not invited]
    Tatsuya Ishihara; Ryota Kobayashi; Manato Asai; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Hirofumi Kawazumi; Tatsuyuki Nakatani; Keishi Okamoto
    第60回高分子学会年次大会  2011/05
  • Preparation of substrates surface-modified with anti-body binding poly(MPC) and evaluation of cell specific attachment on their surfaces  [Not invited]
    Akihiro Tominaga; Akinori Imajo; Keita Yamasaki; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama
    第60回高分子学会年次大会  2011/05
  • Preparation of poly (L-lactic acid) / natural rubber composites reinforced with epoxidized natural rubber and polycarbodiimide for improvement of the thermal and impact mechanical properties  [Not invited]
    Shusaku Sagara; Dai Harada; Nozomu Tchiyoda; Keiko Sakaguchi; Toru Yano; Kohei Shiraishi; Kazuo Sugiyama
    第59回高分子討論会  2010/09
  • Preparation of MPC polymer having antibody binding sites for the isolation of targeting cells using a single cell-microarray and immobilization of its polymer on various substrates  [Not invited]
    Ryota Kobayashi; Akihiro Tominaga; Tatsuya Ishihara; Akinori Imajo; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第59回高分子討論会  2010/09
  • Preparation of thermosensitive surface with PNiPAAm and PMPC for fabrication of a cell microarray and thermal-stimuli detachment of cells on its surface  [Not invited]
    Ryota Kobayashi; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第59回高分子学会年次大会  2010/05
  • Development of novel anti-freezing paint by using polyioncomplex  [Not invited]
    Ryohei Shimoji; Yoshiaki Yanagioka; Kohei Shiraishi; Kazuo Sugiyama
    第59回高分子学会年次大会  2010/05
  • Effect of the addition of poly(carbodiimide) as a hydrolysis retarder on thermal and mechanical properties of poly (L-lactic acid) composite consisting of natural rubber and epoxidized natural rubber  [Not invited]
    Dai Harada; Shusaku Sagara; Toru Yano; Keiko Sakaguchi; Kunihiko Hashimoto; Kohei Shiraishi; Kazuo Sugiyama
    第59回高分子学会年次大会  2010/05
  • 温度応答性高分子を表面にもつ細胞アレイの調製と細胞接着，剥離挙動  [Not invited]
    白石 浩平
    日本化学会第90回年次大会  2010/03
  • 多分岐ポリマーを基材とするポリウレタンヒドロゲル膜の調製とフィルム性状の評価  [Not invited]
    白石 浩平
    日本化学会第90回年次大会  2010/03
  • UV吸収性をもつ生体適合性ヒドロゲルの調製とキャラクタリゼーション  [Not invited]
    白石 浩平
    日本化学会第90回年次大会  2010/03
  • 天然ゴム複合化による高強度ポリ乳酸樹脂の開発  [Invited]
    白石 浩平
    関西H&I研究会第24回研究会  2010/03
  • 感温性高分子／ゲルを用いた細胞診断・セパレータ用マイクロアレイの開発  [Invited]
    白石 浩平
    第9回高分子学会中国四国支部高分子材料研究会  2009/10
  • 刺激応答性高分子を表面修飾した細胞診断・セパレータ用マイクロアレイの開発  [Not invited]
    白石 浩平
    第2回産学官連携新産業創出研究会  2009/10
  • Preparation of poly (L-lactic acid) composites reinforced with micro-phase separated natural rubber containing epoxidized natural rubber for improvement of their impact mechanical properties  [Not invited]
    Shusaku Sagara; Dai Harada; Toru Yano; Keiko Sakaguchi; Kohei Shiraishi; Kazuo Sugiyama
    第58回高分子討論会  2009/09
  • Preparation of cell microarray with single-cell attachment and detachment from thermo-responsible polymer surface  [Not invited]
    Ryota Kobayashi; Yasunori Yone; Tatsuyuki Nakatani; Keishi Okamoto; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama
    第58回高分子討論会  2009/09
  • Surface modification with biocompatible polymers onto functional group containing carbon thin film coating substrate by Ar plasma treatment post/graft polymerization technique or condensation with COOH or NH2 terminated polymer and characterization of the  [Not invited]
    Keita Yamasaki; Ryoji Hamawaki; Tatsuyuki Nakatani; Keishi Okamoto; Yuki Nitta; Kohei Shiraishi; Kazuo Sugiyama
    第58回高分子討論会  2009/09
  • Preparation of natural rubber / poly (L-lactic acid) composite reinforced with epoxidized natural rubber for improvement of thermal and mechanical properties of poly(L-lactic acid)  [Not invited]
    Shusaku Sagara; Dai Harada; Toru Yano; Keiko Sakaguchi; Kohei Shiraishi; Kazuo Sugiyama
    第58回高分子学会年次大会  2009/05
  • Preparation of cell microarrays with both thermosensitive poly(HPMA-co-MMA) as a cell attachment domain and PMPC as a cell non-attachment domain, and behavior of cell attachment or thermal-stimuli detachment on its surface  [Not invited]
    Yasunori Yone; Ryota Kobayashi; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第58回高分子学会年次大会  2009/05
  • Surface modification with biocompatible polymers onto nitrogen containing carbon thin film coated aluminum substrate by Ar plasma treatment post/graft polymerization technique or condensation with carboxylic acid terminated polymer and characterization  [Not invited]
    Ryouji Hamawaki; Keita Yamasaki; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto; Yuki Nitta
    第58回高分子学会年次大会  2009/05
  • 自動車内装部品としての高強度・高耐熱性バイオプラスチックの開発  [Not invited]
    白石 浩平
    研究公開フォーラム  2008/10  近畿大学工業技術研究所  研究公開フォーラム
  • Improvement of mechanical and thermal properties of poly (L-lactic acid) by compounding epoxidized natural rubber  [Not invited]
    Shusaku Sagara; Ken Ishibashi; Toru Yano; Keiko Sakaguchi; Kohei Shiraishi; Kazuo Sugiyama
    第57回高分子討論会  2008/09
  • Preparation of New Hydrogel Polyurethane Films based on Hyper Branched Polymer and their Application to Anti-Fouling Paint.  [Not invited]
    Akihito Yokoyama; Kohei Shiraisi; Kazuo Sugiyama; Koji Ohga
    第57回高分子討論会  2008/09
  • Development of biocompatible hydrogel polymer with UV absorber and their characterization  [Not invited]
    Kota Yamasaki; Miho Hirano; Kohei Shiraishi; Kazuo Sugiyama
    第57回高分子討論会  2008/09
  • Preparation, cell attachment and thermal exfoliation on the surface of cell array patterned with thermosensitive poly(N-2-hydroxypropyl- methacrylamide- co-methyl methacrylate) and PMPC  [Not invited]
    Yasunori Yone; Yumi Kanakura; Takuji Yamamoto; Tatsuyuki Nakatani; Keishi Okamoto; Yasue Yamada; Kohei Shiraishi; Kazuo Sugiyama
    第57回高分子討論会  2008/09
  • Chemical modification of thin carbon coating metallic surfaces with functional groups and blood compatibility  [Not invited]
    Ryouji Hamawaki; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第55回高分子討論会  2008/09
  • Chemical modification of thin carbon coating metallic surfaces with functional groups and their surface characterization  [Not invited]
    Ryouji Hamawaki; Kouhei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第55回高分子学会年次大会  2008/05
  • Cell attachment on cell array modified with thermosensitive polymer and thermal stimuli-cell exfoliation on the surface  [Not invited]
    Yasunori Yone; Yumi Kanakura; Takuji Yamamoto; Tatsuyuki Nakatani; Keishi Okamoto; Kohei Shiraishi; Kazuo Sugiyama
    第55回高分子学会年次大会  2008/05
  • Improvement of mechanical and thermal properties of poly (L-lactic acid) by compounding natural rubber and their compatibilizer  [Not invited]
    Shusaku Sagara; Ken Ishibashi; Keiko Sakaguchi; Toru Yano; Kohei Shiraishi; Kazuo Sugiyama
    第57回高分子学会年次大会  2008/05
  • 官能基を導入した炭素質薄膜表面への科学修飾と表面性状の評価  [Not invited]
    白石 浩平
    第25回プラズマプロセシング研究会  2008/01
  • 相反する性質のセグメントをもつブロックコポリマーの調製と機能性素材としての応用  [Not invited]
    白石 浩平
    広島ゴム技術員会  2007/10  広島市工業技術センター  広島ゴム技術員会
  • Characterization of surface-modified thermoresponsible polymer on PET film by the analysis of peptides bound to their surfaces using bacterial random peptide library method  [Not invited]
    Yusuke Kojitani; Tetsuyuki Shigesada; Kohei Shiraishi; Kazuo Sugiyama; Kazuhiko Yamamoto
    第56回高分子討論会  2007/09
  • Immobilization of hydrophilic polymer on thin carbon coating surface with different ratio of sp2/sp3 carbon hybridization by plasma irradiation-post polymerization technique  [Not invited]
    Ryouji Hamawaki; Tatsuyuki Nakatani; Keishi Okamoto; Kohei Shiraishi; Kazuo Sugiyama
    第56回高分子討論会  2007/09
  • Preparation of cell microarray surface-modified with thermoresponsible polymer and polyethyleneglycol for preventing cell attachment  [Not invited]
    Yumi Kanakura; Tatsuyuki Nakatani; Keishi Okamoto; Kohei Shiraishi; Kazuo Sugiyama; Yasunori Yone
    第56回高分子討論会  2007/09
  • Preparation of block copolymer consisting of poly(D-lactic acid) segment having hydrophilic groups as a crystallizer and propyleneglycol / succinic acid copolymer segment as a compatibilizer and improvement of mechanical and thermal properties of  [Not invited]
    Ken Ishibashi; Yukio Kouzai; Syuusaku Sagara; Kohei Shiraishi; Kazuo Sugiyama; Tooru Yano
    第56回高分子討論会  2007/09
  • Evaluation of surface properties of PET film modified with thermoresponsible poly(N-2-hydroxypropylmethacrylamide-co-alkyl methacrylate) copolymer using bacterial random peptide library method  [Not invited]
    Yusuke Kojitani; Tetsuyuki Shigesada; Tatsuya Iguchi; Kazuhiko Yamamoto; Kohei Shiraishi; Kazuo Sugiyama
    第56回高分子学会年次大会  2007/05
  • Preparation of cell array surface-modified with thermoresponsible poly(N-2-hydroxypropyl  [Not invited]
    Yumi Kanakura; Yasunori Yone; Tatsuyuki Nakatani; Keishi Okamoto; Kohei Shiraishi; Kazuo Sugiyama
    第56回高分子学会年次大会  2007/05
  • Immobilization of hydrophilic polymer on thin carbon coating surface by plasma treatment-post polymerization technique  [Not invited]
    Maki Nishioka; Ryouzi Hamawaki; Shingo Wakisaka; Kohei Shiraishi; Kazuo Sugiyama; Tatsuyuki Nakatani; Keishi Okamoto
    第56回高分子学会年次大会  2007/05
  • Molecular assembly and blood compatibility of amphiphilic block copolymers  [Not invited]
    Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    第56回高分子学会年次大会  2007/05
  • Preparation of poly (D-lactic acid) introduced with hydrophilic moieties as a novel crystallizer for improvement of heat and impact properties of poly (L-lactic acid)  [Not invited]
    Ken Ishibashi; Yukio Kouzai; Tooru Yano; Kohei Shiraishi; Kazuo Sugiyama
    第56回高分子学会年次大会  2007/05
  • Preparation of block copolymer with branched poly(D-lactic acid) as crystallizing segment and improvement of mechanical and thermal properties of poly(L-lactic acid)  [Not invited]
    yukio kouzai; ken isibasi; tooru yano; kohei siraisi; kazuo sugiyama
    第55回高分子討論会  2006/09  富山  高分子学会
  • Preparation of vinylcopolymer with zwitterionic L-Lysine as fibrinolytic factor binding sites and phophatidylcholine moieties and inner coating in a narrow tube by a nonthermal plasma flow at atmospheric pressure  [Not invited]
    Shingo Wakisaka; Tatsuyuki Nakatani; Keishi Okamoto; Kohei Shiraishi; Kazuo Sugiyama
    第55回高分子討論会  2006/09  富山  高分子学会
  • Biological characterization of polymer material surface by bacterial random peptide library method  [Not invited]
    Tetsuyuki Shigesada; Yusuke Kojitani; Kazuhiko Yamamoto; Kohei Shiraishi; Kazuo Sugiyama
    第55回高分子学会  2006/09  富山  高分子学会
  • Preparation of block copolymers composed of hydrophilic and hydrophobic segment using novel polydimethylsiloxane type polymer azo-initiators  [Not invited]
    Nobuyuki Tanigawa; Kohei Shiraishi; Kazuo Sugiyama
    第55回高分子討論会  2006/09  富山  高分子学会
  • Development of moldable porous hydroxyapatite by using hybrid polymer microsphere  [Not invited]
    takayuki imoto; yuka senda; kohei shiraishi; kazuo sugiyama
    第55回高分子討論会  2006/09  富山  高分子学会
  • Preparation of novel block copolymer with poly(L-lactic acid) crystallizing segment and improvement of mechanical and thermal properties of poly(L-lactic acid)  [Not invited]
    yukio kouzai; tooru yano; kohei shiraishi; kazuo sugiyama
    第54回高分子学会年次大会  2006/05  名古屋  高分子学会
  • Preparation of porous hydroxyapatite by using surface modified polymethylmethacrylate microsphere with phosphoric acid moiety  [Not invited]
    takayuki imoto; yuka senda; kohei shiraishi; kazuo sugiyama
    第54回高分子学会年次大会  2006/05  名古屋  高分子学会
  • Preparation of copolymer with zwitterionic L-Lysine as plasminogen binding sites and phophatidyl choline moieties and its effect on fibrinolytic activity  [Not invited]
    shingo wakisaka; Kohei Shiraishi; kazuo sugiyama
    第54回高分子学会年次大会  2006/05  名古屋  高分子学会
  • 親水性ポリマー-ポリジメチルシロキサンからなるブロックコポリマーのキャラクタリゼーション  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2006/05  名古屋  高分子学会
  • Biological characterization of thermoresponsible polymer grafted PET surface by bacterial random peptide library method  [Not invited]
    Tetsuyuki Shigesada; Yusuke Kojitani; Kazuhiko Yamamoto; Kohei Shiraishi; Kazuo Sugiyama
    第54回高分子学会年次大会  2006/05  名古屋  高分子学会
  • Satozaki Jyunji; Yamamoto Kazuhiko; Shiraishi Kohei; Sugiyama Kazuo
    2005/09  山形  第54回高分子学会討論会
     
    poly(N-α-methacrylamide-L-lysine)[poly(α-LysMA)]あるいはpoly(N-α-acrylamide-L-lysine)[poly(α-LysMA)]をグラフト固定化したフィルム表面へのタンパク質の吸着挙動をタンパク質に特異的な抗原－抗体反応を利用して評価した。フィブリノーゲンやプラスミノーゲンアクチベータインヒビター等は本表面には吸着しにくく、線溶因子プラスミノーゲンおよび組織プラスミノーゲンアクチベータは強く吸着することを認めた。このとき、吸着量はpoly(α-LysMA)よりもpoly(α-LysAA)が増大することを認めた。さらにフィルム表面の抗血栓性をフィブリン平板法で比較検討した。
  • Shigesada Tetsuyuki; Yamamoto Kazuhiko; Shiraishi Kohei; Sugiyama Kazuo
    2005/09  山形  第54回高分子学会討論会
     
    温度応答性poly(N-2-hydroxypropylmethacrylamide-co-methyl methacrylate)あるいはpoly(N-isoporpylacrylamide)を表面固定化した材料の相転移温度の上下による表面性状の差異を菌体表面に12ペプチドシーケンス発現させた大腸菌ランダムライブラリーの選択・解析から評価した。その結果、いずれも相転移温度より高い温度では疎水性アミノ酸の選択確率が、相転移温度以下では親水性アミノ酸の選択確率が増大した。また、温度応答性素材による相転移温度上下での親･疎水性に差異があることを認めた。
  • Maeda Kazuma; Shiraishi Kohei; Sugiyama Kazuo
    2005/09  山形  第54回高分子学会討論会
     
    温度応答性poly(2-hydroxypropyl-co-methyl methacrylate)[poly(HPMA-co-MMA)]をグラフトした汎用樹脂フィルムにさらに、N-methacryloylsuccinimde(NASu)を連続グラフト重合し、温度応答性層と細胞接着性ペプチドRGDS層をもつ二層フィルムを調製した。表面の固定化するRGDS量によってHeLa細胞の接着性は変化した。RGDS層をもつフィルム表面では無接着因子培地では未処理フィルムでは増殖しなかったHeLa細胞が増殖する細胞応答等を示した。
  • 白石 浩平; 鈴木 克之; 仲宗根 薫; 岡田 芳治
    平成17年度（第53回）日本工学教育協会  2005/09  広島  平成17年度（第53回）日本工学教育協会
     
    平成15年度文部科学省サイエンス・パートナーシッププログラム採択事業についての研究成果を報告した。 動機付け教育として実験、実習が効果的であり、生徒・学生の学習活動が継続的に行なわれれば、自学自習によって高度な教育が可能であることが示唆された。同時に実験・実習内容の精査やプログラム以前での実験技術に拘わる事前実習やティーティングアシスタントの養成が重要であることを指摘した。
  • Wakisaka Shingo; Oga Koji; Shiraishi Kohei; Sugiyama Kazuo
    2005/09  山形  第54回高分子学会討論会
     
    側鎖にL-リジン残基をもつメタクリルおよびアクリルアミドを合成した。得られたポリマーと血漿タンパク質との相互作用を表面プラズモン共鳴(SPR)を用いて検討した。
  • 杉山 一男; 白石 浩平; 里崎 順二; 西岡 真希; 脇坂 真伍
    The 8th SPSJ International Polymer Conference(IPC2005)  2005/07  福岡  The 8th SPSJ International Polymer Conference(IPC2005)
     
    炭素質膜表面や汎用樹脂材料への両性イオン構造のpoly(Nα-methacrylamide-L-lysine)のグラフト反応をプラズマ処理－ポスト重合法によって行った。表面の抗血栓性を家兎全血接触試験等から評価した。
  • ando akira; Nishioka Maki; Shiraishi Kohei; Sugiyama kazuo
    2005/05  横浜  第54回高分子学会年次大会
     
    両性イオン構造のD-SerあるいはL-Serを含むpHおよび温度応答性高分子IPN膜を調製した。膜の電位に及ぼすゲルの膨潤－収縮挙動あるいは水中のpHの影響ならびにL-Glu、D-Glu等のキラル認識性能について検討した。
  • sigesada tetsuyuki; nakayama syuji; Yamamoto Kazuhiko; Shiraishi Kohei; Sugiyama Kazuo
    2005/05  横浜  第54回高分子学会年次大会
     
    温度応答性のPoly(2-hydroxypropylmethacrylamide-co-methyl methacrylate)[Poly(HPMA-co-MMA)]をプラズマ処理－ポスト重合法によって固定化したポリエチレンテレフタレート(PET)フィルム表面性状の評価を試みた。菌体表面に12残基のランダムなペプチドシーケンスをもつ大腸菌ライブラリーin vitroでフィルムと接触させ、選択さらたペプチドシーケンスを解析した。その結果、未処理のPETフィルムでは温度による選択アミノ酸の変化を認めなかったが、Poly(HPMA-co-MMA)の相転移温度以上では疎水的なアミノ酸の選択確率が上昇し、相転移温度以下では親水的なアミノ酸の選択確率が増大する表面性状変化を認めた。
  • Maeda Kazuma; Hirano Miho; Mishima Kanako; Shiraishi Kohei; Sugiyama Kazuo
    2005/05  横浜  第53回高分子学会年次大会
     
    温度応答性Poly(2-hydroxypropylmethacrylamide-co-methyl methacrylate)[Poly(HPMA-co-MMA)]をプラズマ処理－ポスト重合法によって汎用樹脂フィルム表面にグラフトした。さらに、細胞接着因子RGDSあるいはフィブロネクチンを固定化するためN-methacryloylsuccindime(MASu)を連続的にプラズマ処理－ポスト重合によって修飾して温度応答性層と細胞接着層の二層構造からなる刺激応答性フィルムを調製した。HeLa細胞の接着および増殖と温度刺激剥離について検討した。
  • Satozaki Jyunji; Wakisaka Shingo; Yamamoto Kazuhiko; Shiraishi Kohei; Sugiyama Kazuo
    2005/05  横浜  第54回高分子学会年次大会
     
    医用材料として多用されるポリ塩化ビニル樹脂等の汎用樹脂表面にプラズマ処理－ポスト重合法によってPoly(Nα-methacrylamide-L-lysine)[Poly(α-LysMA)]を固定化した。表面への血漿タンパク質の吸着性をタンパク質特異的な抗体を用いて評価した。線溶タンパク質プラスミノーゲンや組織プラスミノーゲンアクチベータは強く吸着したが、プラスミノーゲンアクチベータインヒビターやフィブリノーゲンは吸着しにくい表面性状を認めた。さらにフィブリン平板法によりグラフト化表面は線溶機能が亢進された。
  • Yanase Shogo; Wakisaka Shingo; Shiraishi Kohei; Sugiyama Kazuo
    2005/05  横浜  第54回高分子学会年次大会
     
    Poly(Nα-methacrylamide-L-lysine)[Poly(α-LysAA)]を新規に調製し、構造類似体のPoly(Nα-acrylamide-L-lysine)[Poly(α-LysMA)]と線溶タンパク質プラスミノーゲン(Plg)あるいは組織プラスミノーゲンアクチベータ(t-PA)との相互作用について表面プラズモン共鳴(SPR)測定による検討を行った。その結果、これらの線溶タンパク質との相互作用がPoly(α-LysMA)]より強いPoly(α-LysAA)]はPlg/t-PA系での線溶活性をPoly(α-LysMA）よりも亢進した。
  • ODAN MEGUMI; TUJIMOTO MASASI; MAEDA KAZUMA; SIRAISI KOUHEI; SUGIYAMA KAZUO
    2004/09  北海道  第53回高分子学会討論会
     
    2-hydroxypropylmethacrylamide(HPMA)に温度・光応答性を付与した新規ポリマーを調製した。光および温度応答性機能を細胞増殖・剥離用基材として応用するために、本ポリマーをプラズマ処理－ポスト重合によって固定化した汎用樹脂表面にてHeLa細胞あるいはNIH3T3細胞を用いて接着・増殖あるいは光・温度刺激剥離性能について検討した。
  • Kohta Masushi; Satozaki Jyunji; Shiraishi Kohei; Sugiyama Kazuo
    第53回高分子学会討論会  2004/09  北海道  第53回高分子学会討論会
     
    Poly(N-α-methacrylamide-L-lysine)[Poly(α-LysMA)]の線溶タンパク質プラスミノーゲンあるいは組織プラスミノーゲンアクチベータとの相互作用を生体分子間相互作用解析から検討した。Poly(α-LysMA)はこれら線溶タンパク質に特異的な相互作用を認めたが、アルブミン、γ-グロブリン、フィブリノーゲン等の血漿タンパク質との相互作用は殆ど認められなかった。
  • Asami Gou; Satozaki Jyunji; Shiraishi Kohei; Sugiyama Kazuo
    2004/09  北海道  第53回高分子学会討論会
     
    抗凝血活性と生体親和性を併せもつ生医学材料の調製を目的に両性イオン構造のL-Ser基を含むメタクリレート体にアニオン成分としてのメタクリル酸の共重合体を調製した。共重合体のメタクリル酸共重合比がトロンビンの活性に及ぼす影響を酵素反応速度論的に検討した。
  • SATOZAKI Jyunji; ASAMI Gou; SHIRAISHI Kohei; SUGIYAMA Kazuo
    第53回高分子学会年次大会  2004/05  神戸  第53回高分子学会年次大会
     
    O-methacryloyl-L-Serine(L-SerMA)をn-BuLiでアニオン重合した。得られたポリマーの水溶液での性質ならびに生体親和性を知るためにタンパク質との相互作用を蛍光分光法で検討した。
  • HARUHARA Yoshihiro; WAKISAKA Shingo; SHIRAISHI Kohei; SUGIYAMA Kazuo
    第53回高分子学会年次大会  2004/05  第53回高分子学会年次大会
     
    両性イオン構造のL-Ser基を含む温度応答性ポリ（N-イソプロピルアクリルアミド)(PNIPAAm-co-SerMA)の水中での温度応答性の及ぼすpHおよび塩濃度の影響について検討した。
  • Proliferation and exfoliation of HeLa cells on thermo and light sensitive poly(N-2- hydroxypropylmethacrylamide) grafted PET  [Not invited]
    MAEDA Kazuma; OHDAN Megumi; TUJIMOTO Masashi; SHIRAISHI Kohei; SUGIYAMA Kazuo
    2004/05  神戸  第53回高分子学会年次大会
     
    銅クロロフィリン錯体および2-ヒドロキシプロピルメタクリルアミド(HPMA)とMMA共重合体をポリエチレンテレフタレート表面にプラズマ処理－ポスト重合法により表面修飾した。得られたグラフト化フィルム表面でのHeLa細胞の増殖と剥離を検討した結果、表面で接着・増殖した細胞を光または温度刺激によって剥離できることを認めた。
  • ANDO Akira; SHIRAISHI Kohei; SUGIYAMA Kazuo
    第53回高分子学会年次大会  2004/05  神戸  第53回高分子学会年次大会
     
    L-SerあるいはD-Serを含む温度応答性の高分子ゲル膜を調製した。pHが膜電位の及ぼす影響ならびに添加アミノ酸のD-,L-等の構造が膜電位に及ぼす影響を検討した。
  • KOHTA Masushi; SATOZAKI Jyunji; SHIRAISHI Kohei; SUGIYAMA Kazuo
    高分子学会  2004/05  神戸  高分子学会
     
    L-リジンを側鎖にもつポリマーをプラズマ処理－ポスト重合法によって汎用樹脂フィルム表面の固定化した。 線溶タンパク質の吸着性を吸着タンパクに特異的な抗体を用いる化学発光法によって評価し、フィブリン平板法を用いる測定から線溶活性を評価した。
  • 共鳴ミラー法(IAsys)を用いるセリン基を含む両性電解質ポリマーと血漿タンパク質との相互作用  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/09  山口  高分子学会
  • L-リジンで修飾した両性電解質ポリメタクリルアミドの血漿タンパク質吸着評価と血栓溶解性  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/09  山口  高分子学会
  • 両性イオン構造のアミノ酸を含むポリマーヒドロゲルのpHおよび温度による膨潤-収縮挙動  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/09  山口  高分子学会
  • 酸化チタン/ヒドロキシアパタイトを表面にもつPMMA微粒子の調製と性質  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/09  山口  高分子学会
  • 細胞増殖基材としての刺激応答性ポリ（2-ヒドロキシプロピルメタクリルアミド）を表面にもつPETフィルムの調製とキャラクタリゼーション  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/09  山口  高分子学会
  • N-イソプロピルアクリルアミドセグメントをもつポリ（2-ヒドロキシプロピルメタクリルアミド）の調製と温度応答性  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/05  名古屋  高分子学会
  • イソプロピルN-イソプロピルフマラメートを含む温度応答性ポリマーの調製  [Not invited]
    白石 浩平; 杉山 一男
    高分子学会  2003/05  名古屋  高分子学会
  • Effect of structure of alkyl group on the phase transition of aqueous poly (N isopropylacrylamide co alkylacrylamide) solution  [Not invited]
    杉山 一男; 白石 浩平; 山門大晃
    第 50 回高分子学会年次大会 (大阪)  2001/05  第 50 回高分子学会年次大会 (大阪)
     
    直鎖あるいは環状のアルキル基を導入した一連のコポリマーを合成し､ 水溶液の相転移に及ぼすアルキル基の構造と組成の影響について検討した結果､ LCST は PNIPAAm より低下し､ かついずれも RAAm 含量の増加とともに低温側へシフトした｡ また､ コポリマーはいずれも LCST 以下で分子集合した｡

MISC

Awards & Honors

  • 中国地域産官学コラボレーションセンター 平成20年度共同研究・技術移転功労賞
     
    受賞者: 白石 浩平
  • 日刊工業新聞社 第1回モノづくり連携大賞NEDO賞
     
    受賞者: 白石 浩平

Research Grants & Projects

  • マイルドプラズマ処理と精密ラミネートによるフッ素樹脂と銅箔を直接接合する低損失基板製造技術の開発
    経済産業省：戦略的基盤技術高度化推進事業
    Date (from‐to) : 2019/06 -2022/03 
    Author : 永島正嗣
  • レーザー光と高速可動ステージの精密制御による高効率細胞融合・自動化装置の開発
    経済産業省：戦略的基盤技術高度化支援事業
    Date (from‐to) : 2012/06 -2015/03 
    Author : 永島正嗣
  • 地域連携による次世代自動車技術の研究
    文部科学省：私立大学戦略研究基盤形成支援事業
    Date (from‐to) : 2009/04 -2014/03 
    Author : 京極秀樹
  • 安心安全な再生医療を実現する細胞回収自動化システムの開発
    経済産業省：地域イノベーション創出研究開発事業
    Date (from‐to) : 2010/06 -2012/03 
    Author : 永島正嗣
  • 診断／制御と分取デバイスとしての細胞マイクロアレイの開発
    日本学術振興会：科学研究費補助金基盤研究(C)
    Date (from‐to) : 2009/04 -2012/03 
    Author : 白石浩平
  • 刺激応答性高分子を表面修飾した細胞診断・分取用マイクロアレイの開発
    日本学術振興会：科学研究費補助金基盤研究(C)
    Date (from‐to) : 2007/04 -2010/03 
    Author : 杉山一男
  • 細胞機能の制御・評価を指向した刺激応答型マテリアルの創製と組織工学材への応用
    日本学術振興会：科学研究費補助金基盤研究(C)
    Date (from‐to) : 2007/04 -2009/03 
    Author : 白石 浩平
  • 線溶因子結合サイトを修飾した人工抗トロンビン高分子による抗凝血材料の開発
    日本学術振興会：科学研究費補助金基盤研究費(C)
    Date (from‐to) : 2002/04 -2004/03 
    Author : 白石浩平
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2002 -2003 
    Author : SHIRAISHI Kohei; OKADA Kiyotaka; MATSUO Osamu; SUGIYAMA Kazuo
     
    Novel polymers modified with zwitterionic L-serine or L-lysine in the side chain, poly-(O-methacryloyl-L-serine)[poly(SerMA)], poly(α-methacrylamide-L-lysine)[poly(α-LysMA)], and poly(ε-methacrylamide-L-lysine) [poly(ε-LysMA)] were prepared as a surface modifier for blood compatible biomaterials such as catheter, dialyzer and so forth, Tn this research, the evaluation of their blood compatibility has been investigated from the point of view of effects of zwitterionic polymers on the denaturation of serum proteins or platelets, on the inhibition of thrombin which plays a central role in the bloodclotting process, on the selective binding of plasminogen which is the primary zymogen in the fibrinolytic pathway, and on the enhancement of fibrinolytic activity of plasmin or plasminogen/tissue type plasminogen activator(t-PA). Poly(SerMA), poly(α-LysMA), and poly(ε-LysMA) showed the low interaction with albumin, γ-globulin, fibrinogen to prevent protein denaturation. Poly(α-LysMA) uncompetitively inhibits thrombin activity. The fibrinolytic activity by plasmin and plasminogen/t-PA, was enhanced in the presence of poly(α-LysMA) showing specific binding to plasminogen, no enhancement of their fibrinolytic activity was observed in the case of poly(SerMA) or poly(ε-LysMA). As a result ofmeasurements, it is considered that zwitterionic polymers having amino acid moiety, especially poly(α-LysMA) as a blood compatible material are applicable for use in the biomedical devices.
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 2002 -2002 
    Author : 井原 辰彦; 仲宗根 薫; 鈴木 克之; 白石 浩平
     
    光触媒の抗菌作用については多くの研究例が報告されているが,いずれも光触媒を機能させるためには紫外線しか利用できないので,抗菌作用について紫外線の効果と光触媒の効果とを区別して議論することは不可能であった。本研究では我々のグループが開発した可視光応答性酸化チタン光触媒を用いることにより,上記の問題点の解決を図った。検討項目は以下の3項目である。 (1)微生物の影響を調査するのに好ましい光触媒の薄膜化 (2)原核微生物として大腸菌を対象に可視光-光触媒の効果を確認 (3)真核微生物として酵母を対象に可視光-光触媒の効果を確認 (1)については,ゾルゲル法をベースとする方法で可視光応答性酸化チタン光触媒の薄膜化に成功した。具体的には,硫酸チタンを原料としてアンモニアで加水分解後,過酸化水素を加えて加水分解物を解コウして得た過酸化チタン前駆体ゾルを得た。薄膜は前駆体ゾルにアンモニアを加え,パイレックスガラス基材表面にスピンコーターでコーティングし,乾燥後,350℃の温度で1時間焼成することで成膜した。(特許出願) (2)可視光応答型酸化チタン光触媒をコーティングしたパイレックスガラス基材表面に大腸菌(E.coli from)を含む水滴を滴下し,4℃で水分が蒸発しない条件で青色および緑色LEDを照射し,光照射による影響を生菌数によって評価した。その結果,LEDを照射した場合のcell numberは,青色LEDでは0.12×10^2,緑色LEDでは0.23×10^2,と光を照射しないときの4.13×10^2と比較すると明らかに減少し,光触媒の効果が確認された。(3)酵母を対象に(2)と同様の実験を行ったところ,光触媒の効果はほとんど見られなかったことから,光触媒の作用は原核細胞には有効であるが,真核細胞には効果は薄いことが示唆された。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 2001 -2002 
    Author : SUGIYAMA Kazuo; OKADA Kiyotaka; MATSUO Osamu; SHIRAISHI Kohei
     
    Tissue engineering is the recreation of a SD-tissue structure using a cell-seeded scaffold as a replacement for injured tissue to restore biological function. The development of an artifacial extracelluer matrix(ECM) enabling a regulation of cells attachment and proliferation by stimuli-response is becoming one of most important research field. The purpose of this project was 1.preparation and characterization of novel stimuli-response (heat, light, and pH) polymer / hydrogel, 2.development of immobilizing technique of these polymers onto commodity polymers, and examination of cells proliferation on the polymer modified matrix and detachment by some stimulus. The following results were obtained; 1-(1)Thermo- or light- sensitive polymers were prepared from biocompatible poly(2-hydroxypropylmethacrylamide)[poly(HPMA)] with azobenzene moieties or chlorophyllin-Cu complex. 1-(2)Thermo- and pH senstive polymer(hydrogel) were also obtained from N-isopropylacrylamide(NiPAAm) and amphoteric o-methacryloyl-L-amino acid. 1-(3)The release of dansyl-s-phenyl alanine as a drug model was regulated by thermo-stimuli from thermo-sensitive poly(HPMA- co-N-methacryloyl-s-phenylalanie methyl ester) gel. 1-(4)The biocompatibility of poly[2-(methacryloyloxy)ethyl phosphorylchline] as an ECM model was dependent on the structure of polymer chain in water and/or molecular weight. 2-(1)The utilization of novel polymethacrylate or polystyrene containing acrylamide derivatives as an adhesive primers was effective for adhesive bonding between non-polar polyolefine and polar material. 2-(2)The thermo- or light-sensitive polymer was grafted onto polypropylene (PP) or polyethyleneterephtharate(PET) film by using Ar plasma irradiation and post polymerization technique. 2-(3)The attachment and detachment of polystyrene microspheres as a cell model was able to regulate photochemically and thermally on the PP film, 2-(4)The proliferation of HeLa cells on the thermo-sensitive poly(HPMA) grafted PET film(g-PET) was almost as much as that of a polystyrene cell culture dish. 2-(5)The degree of thermal detachment of HeLa cells on the g-PET with a decrease in culturing temperature from 37 ℃ to 4 ℃ after the proliferation was much higher than that of thermo-sensitive poly(NiPAAm) grafted PET.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research
    Date (from‐to) : 1997 -1998 
    Author : SUGIYAMA Kazuo; OKADA Kiyotaka; MATSUO Osamu; SHIRAISHI Kohei
     
    1. Polyetherurethaneureas including tetramethyldisiloxane(TMDS) moiety in main chain were obtained frompolyaddition of polytetrahydrofuran to 4, 4'-diphenylmethane diisocyauate in the presence of 3-bis(hydroxypropyl)-tetramethyldisiloxane, using ethylenediamine as a chain extension reagent. It was found that the TMDS moiety was located on the surface of the PEUU-Si film in air and that PEUU-Si adsorbed albumin. 2. Surface modified poly(ethylene terephthalate) (PET) films with the 2-(methacryloyloxy)ethyl phosphoryicholine(MPG), PMPC-g-PET, was prepared by plasma irradiation-post polymerization technique. PMPC-g-PET adsorbsless serum proteins than the original PET film. Lecithin adsorbed on PMPC-g-PET which showed activity for the inhibition of fibrin formation and no adhesion of mouse fibroblasts (L-929) and platelets. 3. Poly(methyl methaciylate) microspheres modified with L-serine and L-proline moieties were prepared by emulsion copolymerization of methyl methacrylate with O-methacryloyl-L-serine or -L-proline. Amino acid moieties located on the surface of particles. It was found that the adsorption of proteins on particle was effectively suppressed. 4. A series of polydimethylsiloxane block copolymers containing PMPC, poly[2-(hydroxyethyl) methacrylate], poly(2, 3-dihydroxypropyl methacrylate), and poly(l-methacryloyloxy dulcitol) as hydrophilic segments were prepared by radical polymerization of corresponding monomers initiated with polydimethylsiloxane type of azo-initiator. It was found in water that the hydrophilic segments migrated to the surface of copolymer which suppressed the adsorption of serum proteins as well as platelets, less than the original polydimethylsiloxane as control. The block copolymer containing PMPC segment showed no adhesion of L-929 and platelets.
  • 日本学術振興会：科学研究費助成事業
    Date (from‐to) : 1989 -1989 
    Author : 白石 浩平

Teaching Experience

  • Advanced Lecture on Biomaterial ChemistryAdvanced Lecture on Biomaterial Chemistry Graduate School of Systems Engineering, Kinki University
  • Polymer ChemistryPolymer Chemistry Faculty of Engineering, Kinki University

Social Contribution

  • Development of polymer biomaterials for practical application of pluripotent stem cell
    Date (from-to) : 2014/08/21
    Role : Lecturer
    Category : Lecture
    Sponser, Organizer, Publisher  : 第45回広島私学研修会
  • Preparation of novel cell operatiing system for regenerative medicine
    Date (from-to) : 2013/01/23
    Role : Lecturer

Academic Contribution

  • Preparation of novel cell operating system for regenerative medicine
    Date (from-to) :2013/07/06
    Organizer, responsible person: Kinki University

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