詳細

永田　恵里奈 （ナガタ　エリナ）

  • 農学部 水産学科　講師
Last Updated :2024/05/14

コミュニケーション情報 byコメンテータガイド

  • コメント

    魚類病原性微生物の感染経路の解明と防除法の構築、病原性細菌がなぜ特定の魚種にのみ感染するのか（宿主特異性）、水中の構造物への付着物形成における細菌の役割の解明を研究しています。

研究者情報

学位

  • 博士(農学)（近畿大学）

ホームページURL

J-Global ID

研究キーワード

  • 食品微生物学   水圏微生物   

現在の研究分野（キーワード）

    魚類病原性微生物の感染経路の解明と防除法の構築、病原性細菌がなぜ特定の魚種にのみ感染するのか（宿主特異性）、水中の構造物への付着物形成における細菌の役割の解明を研究しています。

研究分野

  • ライフサイエンス / 水圏生産科学

経歴

  • 2014年04月 - 現在  近畿大学農学部水産学科講師
  • 2010年04月 - 2014年03月  近畿大学農学部水産学科助教
  • 2008年04月 - 2010年03月  独立行政法人日本学術振興会特別研究員（PD)
  • 2004年04月 - 2008年03月  近畿大学水産研究所COE博士研究員

学歴

  • 1999年04月 - 2004年03月   近畿大学   農学研究科   水産学専攻
  • 1995年04月 - 1999年03月   近畿大学   農学部   水産学科

所属学協会

  • 日本魚病学会   日本微生物生態学会   日本水産学会   

研究活動情報

論文

  • Erina Fujiwara-Nagata; Gentoku Nakase; Kyohei Kuroda; Takashi Narihiro; Mitsuru Eguchi
    Fisheries Science 2024年02月
  • 永田 恵里奈; 河合 雄介; 酒井 明久; 吉岡 百合; 森本 正則; 上野 大介
    におい・かおり環境学会誌 54 6 347 - 358 公益社団法人におい・かおり環境協会 2023年11月 [査読有り]
     
    “ふなずし”は，琵琶湖周辺で漬けられているなれずしの1種である．伝統的な食文化を継承するため，現代人に好まれる製造方法の解明が求められている．本研究では，嗜好性の高いふなずしの生産管理に向け，品評会に出品されたふなずしを対象として細菌叢と香気成分を分析し，官能評価スコアと併せて評価することを目的とした．ふなずしの細菌叢は，Lactobacillus spp.，Lb. acidipiscis，Lb. buchneri，Lb. brevis，Bacillus spp.，Staphylococcus spp.を主要とする6グループに大別できた．ふなずしから検出された香気成分は3グループに分けられ，それぞれ品評会スコアとおよそ対応していた．品評会で高評価を得たふなずしは清酒と共通の香気成分が多く，低評価のものは腐敗臭成分が多く検出され，嗜好性は香気成分でおよそ説明可能であることが示された．一方で細菌叢と香気成分の明確な関係性はみられず，酵母を含めた発酵過程における経時的な解析が必要となった．
  • Fujiwara-Nagata Erina; Naitou-Shibahara Yuki; Kataoka Yoshitaka; Kintsuji Hiroaki; Eguchi Mitsuru
    Fish Pathology 58 3 91 - 98 2023年09月 [査読有り]
     
    Flavobacterium psychrophilum can infect rainbow trout Oncorhynchus mykiss and ayu Plecoglossus altivelis, detrimentally affecting global aquaculture. ​This study aimed to understand the infectivity and host specificity of two F. psychrophilum strains, SG950607 and PH0424, which were isolated from the kidneys of rainbow trout and ayu, respectively. ​Rainbow trout (fry) and ayu (adult and fry) were infected via two methods: bath immersion and intraperitoneal injection. ​We investigated the ability of each F. psychrophilum strain to survive in the serum of non-original host fish species. ​Rainbow trout died upon being infected by PH0424. ​In addition, the complement of the rainbow trout did not show a bactericidal effect against PH0424. ​On the other hand, ayu infected with SG950607 survived, and their complement eliminated this strain. ​The difference in mortality rates is likely associated with the susceptibility of each strain to the complement activity possessed by the host fish.
  • Mio Takeuchi; Erina Fujiwara-Nagata; Taiki Katayama; Hiroaki Suetake
    SCIENTIFIC REPORTS 11 1 2021年04月 [査読有り]
     
    Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish disease caused by Flavobacterium psychrophilum. In spite of its importance, an effective vaccine is not still available. Manipulation of the microbiome of skin, which is a primary infection gate for pathogens, could be a novel countermeasure. For example, increasing the abundance of specific antagonistic bacteria against pathogens in fish skin might be effective to prevent fish disease. Here, we combined cultivation with 16S rRNA gene amplicon sequencing to obtain insight into the skin microbiome of the rainbow trout (Oncorhynchus mykiss) and searched for skin bacteria antagonistic to F. psychrophilum. By using multiple culture media, we obtained 174 isolates spanning 18 genera. Among them, Bosea sp. OX14 and Flavobacterium sp. GL7 respectively inhibited the growth of F. psychrophilum KU190628-78 and NCIMB 1947(T), and produced antagonistic compounds of<3 kDa in size. Sequences related to our isolates comprised 4.95% of skin microbial communities, and those related to strains OX14 and GL7 respectively comprised 1.60% and 0.17% of the skin microbiome. Comparisons with previously published microbiome data detected sequences related to strains OX14 and GL7 in skin of other rainbow trout and Atlantic salmon.
  • Atsushi Urabe; Riou Nagaiwa; Masayuki Imajoh; Erina Fujiwara-Nagata
    NIPPON SUISAN GAKKAISHI 87 1 31 - 39 2021年 [査読有り]
     
    We collected 28 and 10 Flavobacterium psychrophilum strains isolated from dead and diseased ayu Plecoglossus altivelis altivelis, respectively, from several rivers of Kochi Prefecture. A total of 38 strains were identified from 10 gene regions by using the on/off switch assay, polymerase chain reaction-restriction fragment length polymorphism, and multilocus sequence typing. Genotypes of F. psychrophilum isolated from dead ayu were mostly G-C/A/S/52 (22/28) whereas genotypes of F. psychrophilum isolated from diseased ayu were G-C/A/S/52 (6/10) and A-C/A/R/45 (4/10). The number of outbreaks of bacterial cold-water disease (BCWD) caused by F. psychrophilum in ayu in the rivers of Kochi Prefecture increased in 2015 and peaked in 2018. Most F. psychrophilum isolates from dead ayu in 2015-2018 were identified as having genotypes of G-C/A/S/52 (22/24). These results suggest that the outbreak of BCWD in ayu in the rivers of Kochi Prefecture was caused by a genotype of G-C/A/S/52.
  • Erina Fujiwara-Nagata; Yuki Shindoh; Michitaka Yamamoto; Takashi Okamura; Kentaro Takegami; Mitsuru Eguchi
    FISHERIES SCIENCE 85 6 913 - 923 2019年11月 [査読有り]
     
    Flavobacterium psychrophilum can be divided into three genotypes, G-C type (ayu type), A-C type (multi-fish type), and A-T type (salmon-trout type), by two single-nucleotide polymorphisms in the DNA gyrase subunit A (gyrA) gene. We isolated F. psychrophilum from various samples collected from the lower basin of a river flowing into Lake Biwa, as a model for bacterial survey, in Shiga Prefecture in June, September, and December from 2010 to 2013 and investigated their gyrA genotypes. All three types of F. psychrophilum were isolated in June when ayu went up the river from the lake. In September, ayu gathered in the lower river basin for spawning and became high in density, almost all of the isolates were of the G-C genotype and the A-T genotype was never isolated. In December, only the A-T type was isolated from the river samples, when Biwa trout Oncorhynchus masou rhodurus came to the river to spawn and were present in the sampling area. In accordance with the seasonal change of the host fish species in the river, the genotype of F. psychrophilum isolated from the environment seems to have changed as well.
  • Eric Duchaud; Tatiana Rochat; Christophe Habib; Paul Barbier; Valentin Loux; Cyprien Guerin; Inger Dalsgaard; Lone Madsen; Hanne Nilsen; Krister Sundell; Tom Wiklund; Nicole Strepparava; Thomas Wahli; Greta Caburlotto; Amedeo Manfrin; Gregory D. Wiens; Erina Fujiwara-Nagata; Ruben Avendano-Herrera; Jean-Francois Bernardet; Pierre Nicolas
    FRONTIERS IN MICROBIOLOGY 9 2018年02月 [査読有り]
     
    Flavobacterium psychrophilum, the etiological agent of rainbow trout fry syndrome and bacterial cold-water disease in salmonid fish, is currently one of the main bacterial pathogens hampering the productivity of salmonid farming worldwide. In this study, the genomic diversity of the F. psychrophilum species is analyzed using a set of 41 genomes, including 30 newly sequenced isolates. These were selected on the basis of available MLST data with the two-fold objective of maximizing the coverage of the species diversity and of allowing a focus on the main clonal complex (CC-ST10) infecting farmed rainbow trout (Oncorhynchus mykiss) worldwide. The results reveal a bacterial species harboring a limited genomic diversity both in terms of nucleotide diversity, with ~0.3% nucleotide divergence inside CDSs in pairwise genome comparisons, and in terms of gene repertoire, with the core genome accounting for ~80% of the genes in each genome. The pan-genome seems nevertheless "open" according to the scaling exponent of a power-law fitted on the rate of new gene discovery when genomes are added one-by-one. Recombination is a key component of the evolutionary process of the species as seen in the high level of apparent homoplasy in the core genome. Using a Hidden Markov Model to delineate recombination tracts in pairs of closely related genomes, the average recombination tract length was estimated to ~4.0 Kbp and the typical ratio of the contributions of recombination and mutations to nucleotide-level differentiation (r/m) was estimated to ~13. Within CC-ST10, evolutionary distances computed on non-recombined regions and comparisons between 22 isolates sampled up to 27 years apart suggest a most recent common ancestor in the second half of the nineteenth century in North America with subsequent diversification and transmission of this clonal complex coinciding with the worldwide expansion of rainbow trout farming. With the goal to promote the development of tools for the genetic manipulation of F. psychrophilum, a particular attention was also paid to plasmids. Their extraction and sequencing to completion revealed plasmid diversity that remained hidden to classical plasmid profiling due to size similarities.
  • Tatiana Rochat; Erina Fujiwara-Nagata; Segolene Calvez; Inger Dalsgaard; Lone Madsen; Alexandra Calteau; Aurelie Lunazzi; Pierre Nicolas; Tom Wiklund; Jean-Francois Bernardet; Eric Duchaud
    FRONTIERS IN MICROBIOLOGY 8 2017年09月 [査読有り]
     
    Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes. This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups. PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.
  • Paul Barbier; Aurelie Lunazzi; Erina Fujiwara-Nagata; Ruben Avendano-Herrera; Jean-Francois Bernardet; Marie Touchon; Eric Duchaud
    FEMS MICROBIOLOGY LETTERS 348 1 26 - 35 2013年11月 [査読有り]
     
    Phosphorothioate modification of DNA and the corresponding DNA degradation (Dnd) phenotype that occurs during gel electrophoresis are caused by dnd genes. Although widely distributed among Bacteria and Archaea, dnd genes have been found in only very few, taxonomically unrelated, bacterial species so far. Here, we report the presence of dnd genes and their associated Dnd phenotype in two Flavobacterium species. Comparison with dnd gene clusters previously described led us to report a noncanonical genetic organization and to identify a gene likely encoding a hybrid DndE protein. Hence, we showed that dnd genes are also present in members of the family Flavobacteriaceae, a bacterial group occurring in a variety of habitats with an interesting diversity of lifestyle. Two main types of genomic organization of dnd loci were uncovered probably denoting their spreading in the phylum Bacteroidetes via distinct genetic transfer events. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.
  • Erina Fujiwara-Nagata; Celine Chantry-Darmon; Jean-Francois Bernardet; Mitsuru Eguchi; Eric Duchaud; Pierre Nicolas
    VETERINARY RESEARCH 44 1 2013年05月 [査読有り]
     
    The bacterium Flavobacterium psychrophilum is a serious problem for salmonid farming worldwide. This study investigates by multilocus sequence typing (MLST) the population structure of this pathogen in Japan where it is also a major concern for ayu, a popular game fish related to salmoniforms. A total of 34 isolates collected across the country and 80 isolates sampled in a single model river by electrofishing were genotyped. The data accounting for 15 fish species allowed identifying 35 distinct sequence types (ST) in Japan. These ST are distinct from those reported elsewhere, except for some ST found in rainbow trout and coho salmon, two fish that have been the subject of intensive international trade. The pattern of polymorphism is, however, strikingly similar across geographical scales (model river, Japan, world) in terms of the fraction of molecular variance linked to the fish host (∼50%) and of pairwise nucleotide diversity between ST (∼5 Kbp§ssup§-1§esup§). These observations go against the hypothesis of a recent introduction of F. psychrophilum in Japan. Two findings were made that are important for disease control: 1) at least two independent F. psychrophilum lineages infect ayu and 2) co-infections of the same individual fish by different strains occur. © 2013 Fujiwara-Nagata et al. licensee BioMed Central Ltd.
  • E. Fujiwara-Nagata; J. Ikeda; K. Sugahara; M. Eguchi
    JOURNAL OF FISH DISEASES 35 7 471 - 480 2012年07月 [査読有り]
     
    We developed a simple genotyping method for Flavobacterium psychrophilum for analysing two single nucleotide polymorphisms (SNPs) in the gyrA gene and to distinguish between isolates that are virulent and avirulent to ayu, Plecoglossus altivelis altivelis (Temminck & Schlegel). The genotyping method is an on/off switch assay and is based on the polymerase chain reaction technique with phosphorothioated primers. We classified 232 isolates from four families of fish (i.e. Plecoglossidae, Osmeridae, Cyprinidae and Salmonidae) into four genotypes (G-C, A-T, A-C and G-T). The G-C type isolates exhibited strong pathogenicity to ayu, whereas the A-T and G-T types did not show any pathogenicity to this species. The A-C type exhibited no or weak pathogenicity to ayu. These results indicate that genotyping F.similar to psychrophilum isolates with two SNPs from gyrA can clearly distinguish between isolates potentially harmful to ayu (G-C type) and those that are potentially not harmful or less harmful (A-C, A-T and G-T type). The on/off switch assay provides a quick, simple, and very powerful DNA genotyping technique for F.similar to psychrophilum isolates.
  • Erina Fujiwara-Nagata; Mitsuru Eguchi
    Aquaculture Science 60 469 - 474 2012年 [査読有り]
  • Kazuhiro Sugahara; Erina Fujiwara-Nagata; Ayami Fukuda; Mitsuru Eguchi
    FISH PATHOLOGY 45 4 158 - 163 2010年12月 [査読有り]
     
    Bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum leads to heavy mortality of ayu Plecoglossus altivelis in Japan. Previously, a 28 degrees C warmed water treatment was shown to be an effective treatment for BCWD, since F psychrophilum can not grow and maintain colonies at this temperature. However, it was unclear whether the bacteria might resuscitate after the treatment was over. Therefore, we investigated colony formation, membrane potential and pathogenicity of F psychrophilum at various temperatures (15 degrees C, 23 degrees C, 28 degrees C and 33 degrees C) in sterilized underground water. Within 2 days at 28 degrees C, F psychrophilum completely lost their colony-forming abilities but still maintained their membrane potentials. It seemed that these cells entered into viable but non-culturable (VBNC) state. However, experimental ayu infection revealed that VBNC F psychrophilum cells were unable to cause BCWD, suggesting that the cells were progressing towards death at 28 degrees C.
  • 江口 充; 永田 恵里奈; Kazuhiro Sugawara
    Fish Pathology 45 2 58 - 65 2010年06月 [査読有り]
     
    冷水病細菌, Flavobacterium psychrophilum,の感染魚体内及び飼育水中の動態
  • E. Fujiwara-Nagata; M. Eguchi
    JOURNAL OF FISH DISEASES 32 10 873 - 881 2009年10月 [査読有り]
     
    Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout fry syndrome of salmonids. The pathogen has been reported from all regions in the world involved in salmonid aquaculture, but also from natural fresh-water environments. We established a quantitative loop-mediated isothermal amplification of DNA (LAMP) method to estimate quantities of F. psychrophilum. LAMP primers were designed based on the sequence of the DNA topoisomerase IV subunit B gene, parE, of F. psychrophilum. parE LAMP exhibited a high specificity for the parE gene of F. psychrophilum but not for other related species. parE LAMP detected the gene in a wide range of concentrations from 2.0 x 101 to 2.0 x 109 copies/reaction within 70 min and revealed a good correlation between threshold times and gene copy number.
  • Erina Fujiwara-Nagata; Yoko Eguchi; Ryutaro Utsumi; Mitsuru Eguchi
    FISHERIES SCIENCE 73 2 348 - 355 2007年04月 [査読有り]
     
    Vibrio anguillarum kills various kinds of fish over a range of salinities from sea water to fresh water, and causes serious damage to aquaculture systems. In this study, the transcriptional regulation of the Na+-NADH:quinone oxidoreductase (Na+-NQR) operon in V. anguillarum from the logarithmic to stationary phases was investigated. Cloning of the Na+-NQR operon revealed a 7 kb nucleotide sequence composed of six open reading frames with amino acid sequence identity of more than 80% with other Vibrio species. Two promoters, nqrP1 and nqrP2, were identified in the region upstream of the nqrA gene using an S1 nuclease assay. The nqrP1 promoter was constitutively activated throughout the logarithmic to stationary phases and possessed -10 (5'-TAGACT-3') and -35 (5'-ATGGCA-3') sequences, which were similar to the consensus sequence of Escherichia coli. On the other hand, the nqrP2 promoter was activated only at the stationary phase and had only a -10 (5'-CATACT-3') and not a -35 sequence. These results suggest that nqrP2, which works specifically in the stationary phase, contributes to starvation-survival in V. anguillarum.
  • 永田 恵里奈; 江口 充
    日本水産学会誌 73 2 306 - 309 2007年03月 [招待有り]
     
    環境水におけるアユ冷水病菌Flavobacterium psychrophilumの定量的モニタリング．
  • Erina Fujiwara-Nagata; Mitsuru Eguchi
    FEMS microbiology letters 234 1 163 - 7 2004年05月 [査読有り]
     
    Vibrio anguillarum kills various kinds of fish over salinities ranging from seawater to freshwater. In this study, we investigated the role of Na+ in V. anguillarum, especially under energy-depleted conditions such as in natural seawater. V. angustum S14, which is a typical marine vibrio. was used for comparison. V. anguillarum only required Na+ for starvation-survival, but ill contrast, V. angustum S14 always required Na+ for both growth and starvation-survival. In marine vibrios, Na+ is used in the Na+-dependent respiratory chain that produces the sodium motive force (SMF) across the cell membrane. It has been considered that marine vibrios always need a SMF produced by Na+, however ill the case of V. anguillarum, the SMF is not required for growth, but becomes more important for starvation-survival. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
  • The role of Na+ in Vibrio anguillarum, a fish pathogen, in growing phase and starvation-survival
    近畿大学 2004年
  • Erina Fujiwara-Nagata; Kazuhiro Kogure; Kumiko Kita-Tsukamoto; Minoru Wada; Mitsuru Eguchi
    FEMS microbiology ecology 44 2 225 - 30 2003年05月 [査読有り]
     
    The activity of membrane-bound NADH oxidase of Vibrio anguillarum M93 (serotype J-O-1), which causes vibriosis in freshwater area was activated by Na+ in the same manner as other marine Vibrios. However, in addition to Na+, K+ was also found to positively enhance the NADH oxidase activity of strain M93. This tendency has not been recognized in other marine Vibrios. Furthermore, the Na+-dependent NADH oxidase of strain M93 required less Na+ (0.1 M) for its maximum activity than those of other Vibrios such as Vibrio alginolyticus and 'Vibrio angustum' S14, which were in the range of 0.4 M NaCl, similar as seawater. Destruction of H+ motive force by a proton conductor carbonylcyanide m-chlorophenylhydrazone (CCCP) revealed high dependency of V anguillarum on the primary H+ pump. Even at pH 8.5, V anguillarum strains other than serotype O-4 could not grow well with the addition of CCCP. In contrast, marine-type Vibrios such as V. alginolyticus and V. angustum S14 can grow well at pH 8.5 even with the addition of CCCP. The lower requirement for Na+ in V anguillarum probably reflects the salinity of their original habitats. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
  • Erina Fujiwara-Nagata; Mitsuru Eguchi
    Microbes and Environments 18 4 196 - 202 2003年 [査読有り]
     
    Vibriosis caused by Vibrio anguillarum seriously injures freshwater fish (Salmoniforms) almost every year in Lake Biwa, Japan. This pathogen needs NaCl for its growth and survival. When the pathogen was directly exposed to sterilized aged lake water (ALW) at room temperature, it suddenly lost its culturability and pathogenicity, and died within half a day due to the low osmolarity. In this report, the survival of the pathogen as biofilms formed on air-solid and liquid-solid (agar or polystyrene) interfaces in ALW was investigated. When the biofilms formed at air-solid and liquid-solid (agar or polystyrene) interfaces were exposed to ALW at 4-5°C in the dark, the pathogen survived for more than 2 and 4 weeks, respectively. The biofilms at both interfaces at 4-5°C in the dark enhanced the production of a mucous polymer matrix. The main constituent of the polymer was exopolysaccharide. The polymer was produced only in the dark at low temperature. At 20°C, there was no production of the polymer and the survival of the pathogen was shortened. The biofilm seemed to provide a functional consortium to support the survival of V. anguillarum in freshwater. © 2003, Japanese Society of Microbial Ecology & The Japanese Society of Soil Microbiology. All rights reserved.
  • Mitsuru Eguchi; Erina Fujiwara-Nagata; Nobukazu Miyamoto
    Microbes and Environments 18 3 160 - 166 2003年 [査読有り]
     
    Vibriosis caused by Vibrio anguillarum serotype J-O-1 seriously affects the freshwater fish ayu (Plecoglossus altivelis) in Lake Biwa, Japan. Survival patterns of V. anguillarum were investigated in aged lake water (ALW) supplemented with or without 0.75% NaCl. It was found that 0.1-1.0% of V. anguillarum cells maintained the ability to form colonies even after 6 weeks in 0.75% NaCl-ALW. Under the same starved conditions, MPN counts with liquid medium were 100 times higher than CFU counts. When exposed to sterilized aged lake water without NaCl, V. anguillarum entered a non-culturable state within half a day. As the mineralization activity of non-culturable cells was still 0.1-5.4% of that in the culturable phase, this physiological state can be described as “viable but non-culturable”. However, all attempts to return to a culturable state including re-infection were un-successful. The non-culturable cells in ALW lost all pathogenicity in fish. Since a longer exposure to ALW resulted in less mineralization activity, the non-culturable state of V. anguillarum cells in freshwater seems to reflect a phase of decay leading to cell death. However, certain environmental factors such as coldness and microaerobiosis seem to help the pathogen to survive longer in freshwater without a loss of culturability. © 2003, Japanese Society of Microbial Ecology & The Japanese Society of Soil Microbiology. All rights reserved.
  • 魚病細菌Vibrio anguillarumの増殖及び飢餓生残におけるNa+の役割
    近畿大学 2001年
  • Mitsuru Eguchi; Erina Fujiwara; Nobukazu Miyamoto
    Journal of Infection and Chemotherapy 6 2 126 - 129 2000年 [査読有り]
     
    Vibriosis caused by Vibrio anguillarum seriously damages freshwater fish (Salmoniforms) almost every year in Lake Biwa, Japan. This pathogen requires NaC1 for its growth. When the pathogen was exposed to sterilized aged lake water, it lost its culturability without loosing respiratory activity. Although this physiological state appears to be 'viable but nonculturable' (VBNC), the cells could not be resuscitated even in host fish bodies. As these cells totally lost their pathogenicity, the VBNC-like state is probably on the pathway to cell death. Environmental factors such as darkness, coldness, and anaerobiosis helped the pathogen to survive in freshwater without reaching a nonculturable state.

書籍

  • ７章 病原微生物の動態と衛生管理、§１．病原微生物の動態、「養殖の餌と水－陰の主役たち」（杉田治男編）
    江口 充; 永田 恵里奈 (担当:共著範囲:)恒星社厚生閣 2008年

講演・口頭発表等

  • 魚類養殖場の底質悪化と海底の乳酸菌の関係  [通常講演]
    永田恵里奈; 宮本昌佳; 杉 勇佑; 中瀬玄徳; 江口 充
    平成31年度日本水産学会春季大会 2019年03月 ポスター発表
  • Survival strategy of two Flavobacterium psychrophilum lineages, ST52 and ST48, in natural environments  [通常講演]
    Erina Fujiwara-Nagata; Mitsuru Eguchi
    Flavobacterium 2018 2018年11月 口頭発表（一般）
  • 魚を病気にする微生物と魚を美味しくする微生物のお話  [通常講演]
    永田 恵里奈
    中央区民カレッジ 2018年10月 公開講演，セミナー，チュートリアル，講習，講義等
  • 冷水病菌の種類と特徴〜冷水病を引き起こしているのは誰か〜  [招待講演]
    永田 恵里奈
    四万十町冷水病に関する講演 2018年06月 公開講演，セミナー，チュートリアル，講習，講義等
  • 遺伝系統の異なる冷水病菌の魚血清中での生残能力  [通常講演]
    永田恵里奈; 内藤由紀; 高岡拓海; 久保 勝; 片岡佳孝; 金辻宏明; 江口 充
    平成30年度日本水産学会春季大会 2018年03月 口頭発表（一般）
  • 養殖漁場の海底泥に存在する乳酸菌  [通常講演]
    永田恵里奈; 若井 陵; 松岡真史; 中瀬玄徳; 江口 充
    環境微生物系合同大会2017 2017年08月 ポスター発表
  • スナヤツメがアユ型冷水病菌を長期保菌する可能性  [通常講演]
    永田恵里奈; 澤井里美; 江口 充
    平成29年度日本水産学会春季大会 2017年03月 口頭発表（一般）
  • Specific Infectivity of two Flavobacterium psychrophilum strains isolated from different fish species  [通常講演]
    Erina Fujiwara-Nagata; Yuki Naitou; Michitaka Yamamoto; Yoshitaka Kataoka; Mitsuru Eguchi
    Flavobacterium 2015 2015年10月 口頭発表（一般）
  • Nation-wide and local diversity of the fish pathogen Flavobacterium psychrophilum in Japan revealed by MLST analysis  [招待講演]
    Erina Fujiwara-Nagata; Pierre Nicolas; Céline Chantry-Darmon, Jean-François Bernardet; Mitsuru Eguchi; Eric Duchaud
    Flavobacterium 2012 2012年06月 口頭発表（招待・特別）

MISC

産業財産権

共同研究・競争的資金等の研究課題

  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2022年04月 -2025年03月 
    代表者 : 竹内 美緒; 永田 恵里奈
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2021年04月 -2024年03月 
    代表者 : 永田 恵里奈
     
    申請者らは先行研究において、アユ由来冷水病菌が3型O抗原関連遺伝子を持つこと、国内外のニジマス由来冷水病菌の中にはアユ由来冷水病菌と同じ3型O抗原関連遺伝子を持つ株が少数いることを明らかにした。本課題では、3型O抗原関連遺伝子が、アユあるいはニジマスに対する病原性の発揮に重要なのかを解明するために、アユならびにニジマスへの感染試験を行いそれらの病原性の有無を確認した。また、アユ由来で3型以外のO抗原関連遺伝子を持つ冷水病菌も発見されたので、その病原性についても調べた。 供試した冷水病菌は、3型O抗原関連遺伝子を持つ13株（アユ由来：10株、サケ科魚類由来：3株）、0型O抗原関連遺伝子を持つ2株（アユ由来：1株、コイ科魚類由来：1株）、そして2型O抗原関連遺伝子を持つ2株（サケ科魚類由来）の合計17株である。アユ（海産系）の稚魚とニジマスを用いて浸漬感染により攻撃試験を行った。 その結果、3型O抗原関連遺伝子を持つニジマス由来の冷水病菌はニジマスに対してのみ病原性を示し、アユには病原性を示さなかった。3型O抗原関連遺伝子を持つアユ由来の冷水病菌は、1株を除いて全てアユに対してのみ病原性を示し、ニジマスには病原性を示さなかった。0型O抗原関連遺伝子を持つアユ由来の冷水病菌は、ニジマスにもアユにも病原性を示さなかった。0型O抗原関連遺伝子を持つコイ科魚類由来の冷水病菌も同様に、ニジマスにもアユにも病原性を示さなかった。2型O抗原関連遺伝子を持つ2株（サケ科魚類由来）は、アユには病原性を示さなかったが、ニジマスに対しては病原性を示した。
  • 海洋性乳酸菌を用いた養殖漁場悪化の早期発見法の開発
    日本学術振興会：科学研究費助成事業
    研究期間 : 2018年04月 -2020年03月 
    代表者 : 永田 恵里奈
     
    魚類養殖漁場の有機汚濁の程度は、硫化物の発生・蓄積や底生生物の減少といった汚濁の結果で表現されている。海域が持つ自然の自浄作用に頼った漁場管理の場合、汚濁の結果を見ていては手遅れになってしまう。悪化を予防するためには、悪化が起きる予兆を知る指標 の方が役にたつ。申請者らは硫酸還元菌による硫化水素の生成が有機酸（主に乳酸）濃度によって制御されている点に注目し、底泥の乳酸菌数と魚類養殖による負荷（主に硫化物量）との関係を調べてきた。その結果、生簀下の硫化物量が増える前に乳酸菌数が増えることな どを明らかにした。乳酸菌の変動は、硫化物量の増大する前触れとなっている可能性が高い。しかし、乳酸菌の数や種組成の変化と相関関係を示す硫化物量の水準や、両者の変動にどのくらいのタイムスパンがあるのかを含めて未解明な点が残されている。本研究ではこれらの 解明に取り組み、硫化物量が基準値内であっても進行する汚濁を検知する「隠れメタボ診断」の様なツールの開発を目指す。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2011年 -2012年 
    代表者 : 江口 充; 谷口 亮人; 永田 恵里奈
     
    近畿大学のクロマグロ養殖イケスは、奄美大島の花天湾にある。ここのイケスロープには、美しいサンゴが育っている。このクロマグロ養殖とサンゴ共存のメカニズムを物質循環の駆動力となる海洋細菌の動態とサンゴが大量に放出する粘液に注目して調べた。イケス周辺の「海水のみ」と「海水+粘液」を一定時間培養し、比較すると、「海水のみ」ではあまり細菌群集構造が変化しなかったが、「海水+粘液」ではその質も量も大きく変化した。
  • 魚類冷水病菌の宿主特異性決定因子の解明および新型ワクチンの開発
    日本学術振興会優秀若手研究者海外派遣事業
    研究期間 : 2009年08月 -2010年01月 
    代表者 : 永田 恵里奈
  • 魚類の冷水病原因菌Flavobacterium psychrophilumの天然環境からの検出手法の開発
    受託研究
    研究期間 : 2004年 -2009年
  • 魚類の冷水病原因細菌の宿主特異性に関与する因子と天然水域における感染経路の解明
    科学研究費補助金（若手研究(B)）
    研究期間 : 2006年04月 -2008年03月 
    代表者 : 永田 恵里奈
  • 養魚場水域における白点虫の動態把握のための高感度検出系の開発と防除策の確立
    科学技術振興調整費による中核的研究拠点（ＣＯＥ）育成
    研究期間 : 2005年
  • Vibrio属細菌の増殖及び飢餓生残におけるNa+の役割
    科学技術振興調整費による中核的研究拠点（ＣＯＥ）育成
    研究期間 : 1999年
  • Role of Na+ for survival and growth of Vibrio
    SCF System for Establishment and Support of Center's of Excellence
    研究期間 : 1999年

委員歴

  • 2010年04月 - 2014年03月   公益社団法人日本水産学会近畿支部   庶務幹事

担当経験のある科目

  • 食品微生物学近畿大学
  • 水圏微生物学近畿大学

その他のリンク

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