KINDAI UNIVERSITY


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加藤 容子カトウ ヨウコ

プロフィール

所属部署名農学部 バイオサイエンス学科 / 農学研究科
職名教授
学位博士(農学)
専門動物発生工学
ジャンル経営・産業/農林
コメンテータガイドhttp://www.kindai.ac.jp/meikan/144-katou-youko.html
ホームページURLhttp://kaken.nii.ac.jp/d/r/40278742.ja.html
メールアドレスyoko[at]nara.kindai.ac.jp
Last Updated :2017/09/14

コミュニケーション情報 byコメンテータガイド

コメント

    クローン動物作出による全能性誘導やその機構解明、周辺技術の開発といった哺乳動物の発生工学研究に長く携わる。専攻生には習得した技術が活かせる生殖補助医療胚培養士が人気で多くが就業。

学歴・経歴

経歴

  •   2009年,  - 現在, 近畿大学(教授)

研究活動情報

研究分野

  • 動物生命科学, 統合動物科学, 動物発生工学
  • 実験動物学, 実験動物学
  • 畜産学・獣医学, 応用動物科学

研究キーワード

  • reprogramming, 核移植, 初期化, クローン, 全能性

論文

  • Mitogen-Activated Protein Kinase Activity Is Not Essential for the First Step of Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer., Tani T, Kato Y, Cellular reprogramming, 19, 2, 95, 106,   2017年04月, 査読有り
  • Estimating the survival probability of nuclear transfer embryos before embryo transfer by unique biopsy: diagnosing by evaluating Oct4 and Sox2 gene expression pattern on a monozygotic twin blastocyst separated at the 2-cell stage of nuclear transferred e, Kazuki Ohata, Yoko Kato, J.Mamm.Ova.Res, 33, 55, 61,   2016年, 査読有り
  • 核移植とクローン動物, 加藤容子, 脳神経系の再生医学, 161, 165,   2015年
  • Developmental Competence of Vitrified-Warmed Bovine Oocytes at the Germinal-Vesicle Stage is Improved by Cyclic Adenosine Monophosphate Modulators during In Vitro Maturation., Ezoe K, Yabuuchi A, Tani T, Mori C, Miki T, Takayama Y, Beyhan Z, Kato Y, Okuno T, Kobayashi T, Kato K, PloS one, 10, 5, e0126801,   2015年, 査読有り
  • Nuclear Transfer Technologies, Yoko Kato, Yukio Tsunoda, Transgenic Animal Technology: A Laboratory Handbook: Third Edition, 195, 227,   2014年06月23日
    概要:© 2014 Elsevier Inc. All rights reserved. Since the advent of Dolly the Sheep in the 1990s, nuclear transfer or cloning technology has been used extensively to examine the developmental potency of embryonic and somatic cell nuclei. The development of nuclear transfer techniques in mammals has been delayed due to the lack of sufficient techniques and knowledge to manipulate mammalian oocytes, zygotes, and embryos. Because the earlier studies on nuclear transfer in mammals were performed without enucleation of recipient eggs, nuclear transferred eggs did not develop well. As early obstacles, sometimes controversial, were overcome, nuclear transfer technologies have found their place in the genetic engineering of a host of animal species. The pronuclear transplantation technique has been applied to clarify the reason for the developmental failure of mammalian parthenogenetic embryos, and it has demonstrated that both female and male genomes have different roles in normal development to term. Current techniques and instrumentation are outlined in detail.
  • Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro., Salehi M, Kato Y, Tsunoda Y, Zygote (Cambridge, England), 22, 2, 213, 217,   2014年05月, 査読有り
  • Donor Cell Type and Cloning Efficiency in Mammals, Yoko Kato, Yukio Tsunoda, Principles of Cloning: Second Edition, 127, 135,   2013年10月01日
    概要:After the first successful nuclear transfer in mammals in 1983 with pronuclear exchanges, this technique was applied to produce cloned animals using unfertilized oocytes. In 1997 first somatic cell cloning was succeeded in sheep, and after that in more than 20 species, somatic cell nuclear transfer has been succeeded. But the successful rate to produce healthy cloned animals is still low. In this chapter, whether donor cell types affect on the cloning efficiency is discussed, with two categories such as germ line cell types and somatic cell types as donor. © 2014 Elsevier Inc. All rights reserved.
  • あたらしい技術で家畜をつくる, 加藤 容子, 学術の動向, 学術の動向, 18, 4, 4_62, 4_67,   2013年
  • Nuclear Transfer Technologies, Yukio Tsunoda, Yoko Kato, Transgenic Animal Technology: A Laboratory Handbook: Second Edition, 195, 231,   2012年12月02日
  • Effects of rizoma arisaematis, a traditional Chinese natural medicine, on in vitro development of mouse in vivo zygotes and embryos produced by intracytoplasmic sperm injection and somatic cell nuclear transfer, Takaaki Sugimoto, Yuta Tsuji, Yoko Kato, Yukio Tsunoda, Journal of Mammalian Ova Research, 29, 128, 134,   2012年11月22日
    概要:After screening 408 crude drugs, we found that Rizoma Arisaematis increased the cell numbers of mouse blastocysts developed from in vivo zygotes. We examined the effects of Rizoma Arisaematis on the in vitro development of mouse zygotes and embryos produced by ICSI and SCNT, as well as on fetal development. Mouse zygotes were cultured in media containing a water-soluble extract of Rizoma Arisaematis at various concentrations, and the potential of zygotes to develop into blastocysts and the cell numbers of blastocysts were examined. In addition, the effects of Rizoma Arisaematis on the in vitro and in vivo developmental potential of embryos produced by ICSI and nuclear transfer were examined. In vitro treatment of zygotes with Rizoma Arisaematis increased the cell numbers of blastocysts. The proportions of the blastocysts that implanted and developed into fetuses were slightly higher in the blastocysts which were developed from zygotes treated with Rizoma Arisaematis than those of the control. The Rizoma Arisaematis treatment of mouse fetal fibroblast cells or embryos produced by ICSI and SCNT did not affect the growth of the cells, or the in vitro development of the zygotes. The present study demonstrated that Rizoma Arisaematis improved the quality of embryos developed from in-vivo zygotes, but not that of embryos produced by ICSI and nuclear transfer. © 2012 Japanese Society of Mammalian Ova Research.
  • Administration of cyclosporin A to recipients improves the potential of mouse somatic cell nuclear-transferred oocytes to develop to fetuses., Tsuji Y, Kato Y, Tsunoda Y, Zygote (Cambridge, England), 20, 3, 261, 267,   2012年08月, 査読有り
  • The effect of berberine treatment on the reversibility of the development of mouse zygotes and gametes, and on the fertilization and subsequent development, Takaaki Sugimoto, Yoko Kato, Yukio Tsunoda, Journal of Mammalian Ova Research, 29, 75, 81,   2012年06月01日
    概要:As reported previously, berberine, the main component extracted from Coptis rhizome and Phellodendron, has potential as a contraceptive for animals since berberine has a strong inhibitory effect on the in vitro development of mouse zygotes and on fetal development in vivo. The present study was undertaken to examine the effect of berberine treatment on the reversibility of the development of zygotes and gametes, and on the fertilization and subsequent development in the mouse. The reversibility of the berberine-induced inhibition of the development of mouse zygotes was dependent on the concentration used and treatment period. Berberine treatment did not inhibit the fertilizing capacity of epididymal spermatozoa and the fertilizability of oocytes at the second metaphase stage. The present study demonstrated that in vitro development of mouse zygotes is about 100 times more sensitive to berberine than the in vitro growth of mouse fetal fibroblast cells. The high stability of berberine at low temperatures for at least for 12 months and the high sensitivity of preimplantation embryos to berberine is useful information when considering the administration of berberine to females as a contraceptive. © 2012 Japanese Society of Mammalian Ova Research.
  • Effect of melatonin treatment on the developmental potential of parthenogenetic and somatic cell nuclear-transferred porcine oocytes in vitro., Nakano M, Kato Y, Tsunoda Y, Zygote (Cambridge, England), 20, 2, 199, 207,   2012年05月, 査読有り
  • Slight improvement in full-term development of mouse somatic cell nuclear-transferred embryos by cotransfer of fertilized embryos., Tsuji Y, Kato Y, Tsunoda Y, Cellular reprogramming, 14, 1, 38, 44,   2012年02月, 査読有り
  • Coptis rhizome and Phellodendron bark extracts and berberine inhibit the development of mouse embryos, Yukio Tsunoda, Yoko Kato, Journal of Mammalian Ova Research, 28, 40, 46,   2011年07月18日
    概要:After screening 269 crude drugs for their ability to inhibit the development of mouse zygotes, we found Coptis rhizome and Phellodendron bark to have inhibitory effects. We examined the effects of both extracts and of berberine, a major component of these plants, on in vitro development of zygotes and on fullterm fetal development in the mouse. Mouse zygotes were cultured in medium containing water-soluble extracts of Coptis rhizome or Phellodendron bark, or berberine at various concentrations for 5 days and the potential of zygotes to develop to blastocysts was examined. In addition, superovulated mice were intramuscularly injected with berberine and mated, and examined for the in vivo development of fertilized eggs to blastocysts and full-term fetuses. In vitro development of zygotes to blastocysts was almost completely inhibited when they were cultured in medium containing more than 0.1 μg/ml Coptis rhizome, 10 μg/ml Phellodendron bark, or 0.01 μg/ml berberine chloride or berberine sulfate. When superovulated and mated females received 100 μg berberine chloride once a day for 2 to 14 days, the proportions of recovered blastocysts and full-term fetuses were significantly decreased. The present study indicates the potential use of berberine as a contraceptive for animals.
  • Effect of Human Chorionic Gonadotropin and Progesterone Administration on the Developmental Potential of Mouse Somatic Cell Nuclear-Transferred Oocytes., Tsuji Y, Kato Y, Tsunoda Y, Cell. Reprogram, 12, 2, 183, 189,   2010年04月, 査読有り
  • The effect of the time interval between injection and parthenogenetic activation on the spindle formation and the in vitro developmental potential of somatic cell nuclear-transferred rat oocytes., Mizumoto S, Kato Y, Tsunoda Y, Zygote (Cambridge, England), 18, 1, 9, 15,   2010年02月, 査読有り
  • Role of the donor nuclei in cloning efficiency: can the ooplasm reprogram any nucleus?, Kato Y, Tsunoda Y, The International journal of developmental biology, 54, 11-12, 1623, 1629,   2010年, 査読有り
  • 【細胞核の初期化メカニズム 多能性・全能性獲得のナゾに迫る】 クローン個体の作出からみた「核移植と核のリプログラミング」, 加藤 容子, Medical Bio, 6, 5, 14, 21,   2009年09月
  • The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2'-deoxycytidine., Tsuji Y, Kato Y, Tsunoda Y, Zygote (Cambridge, England), 17, 2, 109, 115,   2009年05月, 査読有り
  • The developmental potential of parthenogenetic and somatic cell nuclear-transferred rat oocytes in vitro., Mizumoto S, Kato Y, Tsunoda Y, Cloning and stem cells, 10, 4, 453, 459,   2008年12月, 査読有り
  • The effects of time of first cleavage, developmental stage, and delipidation of nuclear-transferred porcine blastocysts on survival following vitrification, M. Kawakami, Y. Kato, Y. Tsunoda, Animal Reproduction Science, 106, 402, 411,   2008年07月01日
    概要:The effect of removing cytoplasmic lipid droplets (delipidation) at the 2-cell and developmental stages on the survival of porcine somatic cell nuclear-transferred blastocysts developed from the enucleated oocytes receiving somatic cells from kidney of an adult female after cryopreservation was examined. Vitrification was performed using the Cryoloop method with a small volume of medium (0.5 μl). To select 2-cell embryos with a high potential to develop into blastocysts, the relationship between the timing of the first cleavage and the developmental potential was examined. The potential of nuclear-transferred oocytes to develop into blastocysts in the intermediate-cleavage group (20-24 h after activation, 25%) was slightly or significantly (P < 0.05) higher than that in fast-cleavage (<20 h after activation, 13%) and slow-cleavage groups (>24 h after activation, 5%). Most non-delipidated blastocysts did not survive after thawing (0% for early-stage and 9% for advanced-stage blastocysts), but the survival rate of delipidated blastocysts 48 h after culture (54% and 72%, respectively) was not significantly different from that of non-vitrified blastocysts (80% and 92%, respectively). The survival rate of advanced-stage blastocysts after vitrification was slightly higher than that of early-stage blastocysts. The present study demonstrates that somatic cell nuclear-transferred porcine blastocysts developed from embryos selected at the 2-cell stage can be preserved by vitrification with a small volume of medium if the lipid droplets of the embryos are first removed. © 2007.
  • The effects of trichostatin A on mRNA expression of chromatin structure-, DNA methylation-, and development-related genes in cloned mouse blastocysts., Li X, Kato Y, Tsuji Y, Tsunoda Y, Cloning and stem cells, 10, 1, 133, 142,   2008年03月, 査読有り
  • Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development., Amarnath D, Li X, Kato Y, Tsunoda Y, The Journal of reproduction and development, 53, 6, 1247, 1263,   2007年12月, 査読有り
  • Aging of recipient oocytes reduces the development of cloned embryos receiving cumulus cells., Liu G, Kato Y, Tsunoda Y, The Journal of reproduction and development, 53, 4, 785, 790,   2007年08月, 査読有り
  • Effect of the timing of first cleavage on in vitro developmental potential of nuclear-transferred bovine oocytes receiving cumulus and fibroblast cells., Amarnath D, Kato Y, Tsunoda Y, The Journal of reproduction and development, 53, 3, 491, 497,   2007年06月, 査読有り
  • Aberrant spindle assembly checkpoint in bovine somatic cell nuclear transfer oocytes, Tani T, Kato Y, Tsunoda Y, Front Biosci., 12, 2693, 2705,   2007年01月, 査読有り
  • Bovine oocytes with the potential to reprogram somatic cell nuclei have a unique 23-kDa protein, phosphorylated transcriptionally controlled tumor protein (TCTP), Tani T, Shimada H, Kato Y, Tsunoda Y, Cloning Stem Cells, 9, 2, 267, 280,   2007年, 査読有り
  • Comparative gene expression analysis of bovine nuclear-transferred embryos with different developmental potential by cDNA microarray and real-time PCR to determine genes that might reflect calf normality., Kato Y, Li X, Amarnath D, Ushizawa K, Hashizume K, Tokunaga T, Taniguchi M, Tsunoda Y, Cloning and stem cells, 9, 4, 495, 511,   2007年, 査読有り
  • Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer., Rybouchkin A, Kato Y, Tsunoda Y, Biology of reproduction, 74, 6, 1083, 1089,   2006年06月, 査読有り
  • Comparative studies on the mRNA expression of development-related genes in an individual mouse blastocyst with different developmental potential., Li X, Kato Y, Tsunoda Y, Cloning and stem cells, 8, 3, 214, 224,   2006年, 査読有り
  • Demecolcine-assisted enucleation for bovine cloning, Tani T, Shimada H, Kato Y, Tsunoda Y, Cloning Stem Cells, 8, 1, 61, 66,   2006年, 査読有り
  • Analysis of development-related gene expression in cloned bovine blastocysts with different developmental potential., Li X, Amarnath D, Kato Y, Tsunoda Y, Cloning and stem cells, 8, 1, 41, 50,   2006年, 査読有り
  • Comparative analysis of development-related gene expression in mouse preimplantation embryos with different developmental potential., Li X, Kato Y, Tsunoda Y, Molecular reproduction and development, 72, 2, 152, 160,   2005年10月, 査読有り
  • 【再生医学 クローン・幹細胞から医療へ】 未来技術応用 クローン家畜の作成と利用, 角田 幸雄, 加藤 容子, 綜合臨床, 54, 1, 56, 61,   2005年01月
  • Maintenance of meiotic arrest and developmental potential of porcine oocytes after parthenogenetic activation and somatic cell nuclear transfer., Kawakami M, Kato Y, Tsunoda Y, Cloning and stem cells, 7, 3, 167, 177,   2005年, 査読有り
  • Cryopreservation of bovine somatic cell nuclear-transferred blastocyst: effect of developmental stage, Amarnath Dasari, Yoko Kato, Yukio Tsunoda, The Journal of Reproduction and Development, 50, 5, 593, 598,   2004年10月
  • Cryopreservation of bovine somatic cell nuclear-transferred blastocysts: effect of developmental stage., Amarnath D, Kato Y, Tsunoda Y, The Journal of reproduction and development, 50, 5, 593, 598,   2004年10月, 査読有り
  • Effect of low-temperature bovine ovary storage on the maturation rate and developmental potential of follicular oocytes after in vitro fertilization, parthenogenetic activation, or somatic cell nucleus transfer, S. Matsushita, T. Tani, Y. Kato, Y. Tsunoda, Animal Reproduction Science, 84, 293, 301,   2004年09月01日
    概要:The present study examined the competence of oocytes from bovine ovaries stored at low temperatures for at least 1 day, which is the necessary time period to complete inspection for bovine spongiform encephalopathy. Storage of ovaries at 10°C for 24h did not affect oocyte maturation (68% versus 68%) or the potential of oocytes to develop into day 8 blastocysts after in vitro fertilization (25% versus 27%), parthenogenetic activation (19% versus 25%), or somatic cell nucleus transfer (27% versus 32%) compared with controls. In vitro-fertilized and parthenogenetic oocytes from ovaries stored at 10°C for 48h had a significantly decreased maturation rate and developmental potential, but nucleus-transferred oocytes that received cultured cumulus cells did not (27% versus 32%). Thus, bovine ovaries can be stored at 10°C for at least 24h without decreasing oocyte maturation competence or the developmental potential of in vitro-fertilized, parthenogenetically activated, and somatic cell nucleus-transferred oocytes, at least to the blastocyst stage. The present study provides valuable information with regard to removing bovine ovaries from abattoirs after testing for bovine spongiform encephalopathy. © 2004 Elsevier B.V. All rights reserved.
  • Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells, T. Kobayashi, Y. Kato, Y. Tsunoda, Theriogenology, 62, 854, 860,   2004年09月01日
    概要:The present study examined whether the timing of the first cleavage has an effect on the in vitro and in vivo developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells. First, the timing of the first cleavage and the developmental potential of nuclear-transferred oocytes were examined every hour from 12 to 24 h after the start of culture and compared with in vitro-fertilized oocytes. The developmental potential of in vitro-fertilized oocytes decreased gradually according to the time required for cleavage (84% (32/38) for 15 h to 50% (1/2) for 20 h), but intermediate-cleaved (15-16 h) nuclear-transferred oocytes had a higher potential to develop into blastocysts (55% (17/31) to 67% (45/67) versus 0-43% (6/14)]. Second the nuclear-transferred oocytes were divided into three groups according to the timing of the first cleavage; each group was cultured to blastocysts in vitro, and then transferred to recipients. The potential of intermediate-cleaved oocytes (15-16 h) to develop into blastocysts was significantly higher than fast-cleaved (before 15 h) and slow-cleaved (after 16 h) oocytes (65, 46, and 37%). The proportion of fetuses on Day 10.5 of pregnancy was highest in the intermediate-cleaved group (4 versus 2 and 1%, respectively) and a full-term fetus was obtained from this group. The present study demonstrated that the timing of the first cleavage could be used to determine the potential of nuclear-transferred oocytes with embryonic stem cells to develop to the blastocyst stage in vitro, but not to determine post-implantation viability after transfer to recipients. © 2004 Elsevier Inc. All rights reserved.
  • Effect of nuclear transfer procedures on ES-cell cloning efficiency in the mouse, Akiko Yabuuhi, Keiko Ysuda, Yoko Kato, Yukio Tsunoda, The Journal of Reproduction and Development, 50, 2, 263, 268,   2004年04月
  • Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse., Yabuuchi A, Yasuda Y, Kato Y, Tsunoda Y, The Journal of reproduction and development, 50, 2, 263, 268,   2004年04月, 査読有り
  • Nuclear transfer of adult bone marrow mesenchymal stem cells: developmental totipotency of tissue-specific stem cells from an adult mammal., Kato Y, Imabayashi H, Mori T, Tani T, Taniguchi M, Higashi M, Matsumoto M, Umezawa A, Tsunoda Y, Biology of reproduction, 70, 2, 415, 418,   2004年02月, 査読有り
  • 【卵から親へ 新しい動物の発生のしくみ】 クローン動物の発生のしくみと寿命, 角田 幸雄, 加藤 容子, 遺伝: 生物の科学, 58, 1, 64, 68,   2004年01月
  • Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods., Kawano K, Kato Y, Tsunoda Y, Cloning and stem cells, 6, 2, 67, 72,   2004年, 査読有り
  • Parthenogenesis and nuclear transfer in rabbit oocytes., Tsunoda Y, Kato Y, Methods in molecular biology (Clifton, N.J.), 254, 195, 212,   2004年, 査読有り
  • Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes, Kawakami M, Tani T, Yabuuchi A, Kobayashi T, Murakami H, Fujimura T, Kato Y, Tsunoda Y, Cloning and Stem Cells, 5, 4, 379, 387,   2003年12月, 査読有り
  • Reprogramming of bovine somatic cell nuclei is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity., Tani T, Kato Y, Tsunoda Y, Biology of reproduction, 69, 6, 1890, 1894,   2003年12月, 査読有り
  • 【再生医療】 再生医療にかかわる基本的事項 細胞増殖・分化誘導関連因子 核の初期化とその制御因子, 角田 幸雄, 加藤 容子, 日本臨床, 61, 3, 406, 410,   2003年03月
  • 核の初期化とその制御因子, 角田 幸雄, 加藤 容子, 日本臨床, 61, 3, 406, 410,   2003年03月, 査読有り
    概要:体細胞を核移植した時に生じる核の初期化機構を中心に紹介した。
  • Reprogramming of Bovine Somatic Cell Nuclei is Not Divectly Regulated by Maturation Promorting Factor or Mitogen-Activated Protein Kinase Activity, Tetsuya Tani, Yoko Kato, Yukio Tsunoda, Biology of Reproduction, 69, 6, 1890, 1894,   2003年01月, 査読有り
    概要:ウシ体細胞核移植における体細胞核の初期化は、卵細胞質内のMPF 及びMAPK によって直接制御されない事を明らかにした。
  • Effects of aggregation of nuclear-transferred mouse embryos developed from enucleated eggs receiving ES cells on in vitro and in vivo development, Akiko Yabuuchi, Yoko Kato, Yukio Tsunoda, Journal of Reproduction and Development, 48, 393, 397,   2002年12月01日
    概要:Nuclear-transferred mouse eggs receiving embryonic stem cells develop into blastocysts at a high rate, but their potential to develop into fetuses is very low. Therefore, the present study examined the effects of aggregating of two nuclear-transferred eggs at the 8-cell stage to increase the cell number of nuclear-transferred embryos. The proportion of implantation sites on gestational day 10.5 after transfer of aggregated nuclear-transferred embryos (53%) was significantly higher than after single nuclear transfer (29%) or serial nuclear-transfer (37%). The proportion of implantation sites with fetuses did not differ significantly among the three groups (7 to 17%). These results demonstrate that the low potential of nuclear-transferred embryos with embryonic stem cells to develop into fetuses is not due to the lower cell number of nuclear-transferred embryos.
  • Effect of oxygen tension on the developmental potential of parthenogenetic oocytes and nuclear-transferred porcine oocytes receiving fetal fibroblast cells, Masahiro Kawakami, Tetsuya Tani, Xi Jun Yin, Yoko Kato, Yukio Tsunoda, Journal of Reproduction and Development, 48, 409, 414,   2002年12月01日
    概要:The effects of oxygen tension (5% and 20%) during in vitro culture of oocytes and fetal fibroblast cells on the developmental potential of parthenogenetic and nuclear-transferred porcine oocytes were examined. The potential of parthenogenetic oocytes matured and cultured under different oxygen tensions to develop into blastocysts was not significantly different (5 to 16%). The proportions of enucleated oocytes receiving fetal fibroblast cells that developed into blastocysts were significantly lower when somatic cells were cultured under low oxygen tension (2 and 3%) than under high oxygen tension (7%). The effects of oxygen tension during culture of oocytes and somatic cells on the developmental potential of parthenogenetic and nuclear-transferred oocytes is discussed.
  • 【クローン動物の頻発異常とエピジェネティクス】 クローンウシにおける核の初期化と頻発異常, 角田 幸雄, 加藤 容子, 蛋白質・核酸・酵素, 47, 13, 1797, 1803,   2002年10月
    概要:体細胞の核移植によって,多くのクローンウシが作出されているが,その半数は死亡したり,形態形成異常を示している.社会は,核移植技術が家畜の生産性の向上に果たす役割を認めながらも,現在の未完成の技術で作出した生産物を食料とすることに不安感を示している.現行の核移植技術を用いて作出した核移植卵は,正常な形態をもつ胚盤胞へ高率に発生するが,正常な個体へはごく一部しか発生しない.クローンウシ作出研究の現状と,異常個体が頻発する原因について考察する
  • ブタ体細胞由来核移植の発生能に及ぼす酸素濃度の影響, 角田 幸雄, 川上雅弘, 谷 哲弥, Xi Jun Yin, 加藤 容子, Journal of Reproduction and Development, 48, 409, 414,   2002年09月
    概要:ブタ単為発生卵ならびに核移植卵の発生能に及ぼす気相中の酸素濃度の影響を検討した。(英文)
  • ブタ単為発生卵ならびに胎子繊維芽細胞由来核移植卵の体外発生能に及ぼす酸素気相の影響, 川上 雅弘, 谷 哲弥, YIN Xi Jun, 加藤 容子, 角田 幸雄, The Journal of reproduction and development, The Journal of reproduction and development, 48, 4, 409, 414,   2002年08月01日
  • マウスES細胞由来核移植卵の発生能に及ぼす集合ならびに再核置換の影響, 薮内 晶子, 加藤 容子, 角田 幸雄, The Journal of reproduction and development, The Journal of reproduction and development, 48, 4, 393, 397,   2002年08月01日
  • ブタ単為発生卵ならびに胎子繊維芽細胞由来核移植卵の体外発生能に及ぼす酸素気相の影響(Effect of Oxygen Tension on the Developmental Potential of Parthenogenetic Oocytes and Nuclear-Transferred Porcine Oocytes Receiving Fetal Fibroblast Cells), 川上 雅弘, 谷 哲弥, Jun Yin Xin, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development, 48, 4, 409, 414,   2002年08月
  • マウスES細胞由来核移植卵の発生能に及ぼす集合ならびに再核置換の影響(Effects of Aggregation of Nuclear-transferred Mouse Embryos Developed from Enucleated Eggs Receiving ES Cells on In Vitro and In Vivo Development), 薮内 晶子, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development, 48, 4, 393, 397,   2002年08月
  • Effect of delayed enucleation on the developmental potential of nuclear-transferred oocytes receiving adult and fetal fibroblast cells, Yin XJ, Kato Y, Tsunoda Y, Zygote, 10, 3, 217, 222,   2002年08月, 査読有り
  • Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes., Yin XJ, Tani T, Yonemura I, Kawakami M, Miyamoto K, Hasegawa R, Kato Y, Tsunoda Y, Biology of reproduction, 67, 2, 442, 446,   2002年08月, 査読有り
  • ウサギ体細胞核移植卵の発生能に及ぼす除核方法ならびに成熟条件の影響, 角田 幸雄, Xi Jun Yin, 加藤 容子, Reproduction, 124, 41, 47,   2002年07月
  • Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes, Yin Xi Jun, Tani, T., Kato, Y., Tsunoda,Y, Biology of Reproduction, 67, 442, 446,   2002年07月
  • 動物クローンニングの最近の進歩と問題点, 角田 幸雄, 加藤 容子, Differentiation, 69, 158, 161,   2002年05月
    概要:哺乳類のクローンフォーラムの中で、 再生医療の分野においてもクローニング技術を使用すべきではないとの考えを述べた。 (英文)
  • マウス ES 細胞由来胚盤胞内細胞塊細胞の発生能, 角田 幸雄, 天野朋和, 加藤 容子, Cell Tissue Research, 307, 367, 370,   2002年05月
    概要:マウス ES 細胞由来核移植卵の産子への発生能が低い原因を明らかにした。 (英文)
  • In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green, Takatsugu Yamada, Takatsugu Yamada, Takatsugu Yamada, Masahide Yoshikawa, Seiji Kanda, Yoko Kato, Yoshiyuki Nakajima, Shigeaki Ishizaka, Yukio Tsunoda, Stem Cells, 20, 146, 154,   2002年04月22日
    概要:Background and aims. Embryonic stem (ES) cells have a pluripotent ability to differentiate into a variety of cell lineages in vitro. We have recently found the emergence of cell clusters that show the cellular uptake of indocyanine green (ICG) in the culture of differentiated ES cells. ICG is clinically used as a test substance to evaluate liver function because it is eliminated exclusively by hepatocytes. The aim of the present study was to investigate the hepatic characteristics of ICG-stained cells. Methods. Embryoid bodies (EBs), formed by a 5-day hanging drop culture of ES cells, were allowed to outgrow in the placed culture. Gene expression of hepatocyte markers was analyzed by reverse transcriptase-polymerase chain reaction, and albumin production was examined immunohistochemically. Morphology and cellular components were investigated by electron microscopy. ICG-stained cells were further transplanted into the portal vein of mice. Results. ICG-stained cells appeared around 14 days of the EB culture and formed distinct three-dimensional structures. They were immunoreactive to albumin and expressed mRNAs such as albumin, alpha-fetoprotein, transthyretin, hepatocyte nuclear factor 3 beta, alpha-1-antitrypsin, tryptophan-2,3-dioxygenase, urea cycle enzyme, gluconeogenic enzyme, and liver-specific organic anion transporter-1. An ultrastructural analysis revealed a well-developed system of organelles such as mitochondria, lysosomes, Golgi apparatus, and rough and smooth endoplasmic reticulum. The transplantation of ICG-positive cells into the portal vein resulted in the incorporation into mice livers, where they were morphologically indistinguishable from neighboring hepatocytes. Conclusions. ES cell-derived ICG-positive cells possess characteristics of hepatocytes, and ICG- staining is a useful marker to identify differentiated hepatocytes from EBs in vitro.
  • The developmental potential of the inner cell mass of blastocysts that were derived from mouse ES cells using nuclear transfer technology, T. Amano, Y. Kato, Y. Tsunoda, Cell and Tissue Research, 307, 367, 370,   2002年04月06日
    概要:The present study examined the causes of the low developmental potential of enucleated oocytes that have received ES cells and consequent postnatal death of the young. The inner cell masses (ICM) of nuclear-transferred blastocysts or diploid blastocysts were injected into tetraploid blastocysts (group B) or nuclear-transferred tetraploid blastocysts (group C), respectively. The developmental potential of these groups was compared with tetraploid blastocysts injected with ICM of diploid blastocysts (group A). The potential of reconstituted blastocysts to develop into live young in group B increased slightly (5%) but was significantly lower than that in group A (45%). The rate of postnatal death of young in group B did not decrease. The implantation rate of reconstituted blastocysts in group C was very low and no live fetuses were obtained. The results of the present study indicate that the inferior potential of both ICM and trophectoderm cells of nuclear-transferred blastocysts underlies the low developmental rate of nuclear-transferred oocytes receiving ES cells and the higher rate of postnatal death of ES cell-derived young.
  • マウス ES 細胞から肝様細胞の形成, 角田 幸雄, 加藤 容子, 他4名, 山田高嗣, Stem Cells, 20, 146, 154,   2002年03月
    概要:マウス ES 細胞を分化誘導して、 肝様細胞の形成に成功した。 (英文)
  • マウス ES 細胞から腸様器官の形成, 角田 幸雄, 加藤 容子, 他5名, 山田高嗣, Stem Cells, 20, 41, 49,   2002年02月
    概要:マウス ES 細胞を分化誘導して、 腸様器官の作出に成功した。 (英文)
  • Recent progress and problems in animal cloning, Y. Tsunoda, Y. Kato, Differentiation, 69, 158, 161,   2002年01月01日
    概要:It is remarkable that mammalian somatic cell nuclei can form whole individuals if they are transferred to enucleated oocytes. Advancements in nuclear transfer technology can now be applied for genetic improvement and increase of farm animals, rescue of endangered species, and assisted reproduction and tissue engineering in humans. Since July 1998, more than 200 calves have been produced by nuclear transfer of somatic cell nuclei in Japan, but half of them were stillborn or died within several months of parturition. Morphologic abnormalities have also been observed in cloned calves and embryonic stem cell-derived mice. In this review, we discuss the present situation and problems with animal cloning and the possibility for its application to human medicine. © Blackwell Wissenschafts-Verlag 2002.
  • Animal cloning and nuclear reprogramming, Yukio Tsunoda, Yoko Kato, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 47, 1797, 1803,   2002年01月01日
  • 【細胞の再プログラム化と万能性はどこまで可能か】 クローンウシと再プログラム化, 角田 幸雄, 加藤 容子, 実験医学, 19, 12, 1494, 1498,   2001年08月
    概要:体細胞の核移植によるクローンウシ作出研究の現状を述べた.ウシ初期胚の割球や体細胞由来培養細胞を除核未受精卵へ融合すると,核が初期化されて30〜50%の核移植卵が胚盤胞へ発生し,受胚雌へ移植すると約20%が仔ウシへ発育する.又,体細胞を用いた場合は特に,分娩前後の産仔の死亡や形態形成異常等が高頻度で認められる.体細胞核の初期化は,活性化刺激を与える前の未受精卵細胞質中で生じるが,その分子機構は不明である
  • クローンウシと再プログラム化, 角田 幸雄, 加藤 容子, 実験医学, 19, 12, 1494, 1498,   2001年08月
    概要:体細胞核の初期化機構について紹介した。
  • Full-term development of enucleated mouse oocytes fused with embryonic stem cells from different cell lines, T. Amano, Y. Kato, Y. Tsunoda, Reproduction, 121, 729, 733,   2001年06月05日
    概要:The developmental potential of enucleated mouse oocytes receiving embryonic stem cells from ten lines with either the same or different genetic backgrounds using the cell fusion method was examined in vitro and in vivo. The development of nuclear-transferred oocytes into blastocysts was high (34-88%). However, there was no clear correlation between development into blastocysts after nuclear transfer and the chimaera formation rate of embryonic stem cells. The development into live young was low (1-3%) in all cell lines and 14 of 19 young died shortly after birth. Most of the live young had morphological abnormalities. Of the five remaining mice, two died at days 23 and 30 after birth, but the other three mice are still active at days 359 (mouse 1) and 338 (mice 4 and 5) after birth, with normal fertility. However, the reasons for the abnormalities and postnatal death of embryonic stem cell-derived mice are unknown.
  • Comparison of heat-treated and tetraploid blastocysts for the production of completely ES-cell-derived mice, T. Amano, Y. Kato, Y. Tsunoda, Y. Tsunoda, Zygote, 9, 153, 157,   2001年05月01日
    概要:The present study compared the production efficiency and incidence of postnatal death in mice derived by injecting embryonic stem (ES) cells into either heat-treated blastocysts or tetraploid blastocysts. The proportion of completely ES-cell-derived mice from the tetraploid blastocyst group (3.3%) was significantly higher than that obtained from the heat-treated blastocyst group (1.5%). The incidence of postnatal death was the same between the two groups: 10 of 15 young (67%) in the heat-treated group and 21 of 34 young (62%) in the tetraploid group died within 13 days of birth. The remaining young grew to adulthood, had normal fertility, and their germ cells were of ES cell origin. There was no clear correlation, however, between the postnatal lethality of ES-cell-derived mice and the genetic background of the ES cells. The causes of postnatal death are discussed.
  • 【生殖医学のゆくえ】 体細胞クローン技術の新展開, 角田 幸雄, 加藤 容子, HORMONE FRONTIER IN GYNECOLOGY, 8, 1, 33, 38,   2001年03月
    概要:1997年2月に成羊の体細胞の核移植によって子羊を得たことが報告されて以来,その再現性を確かめるための研究が行われてきた.その結果,体細胞を操作して個体が作出できることは確実になったが,成功率は低く,また必ずしも正常な個体が得られるばかりでなく,死産や産子の分娩直後の死亡,形態形成異常などが高率にみられることも明らかになった.体細胞クローン個体作出研究の現状と問題点を明らかにすると共に,医学との接点についても言及した
  • Nuclear transfer of mouse follicular epithelial cells pretreated with spermine, protamine, or putrescine, A. Yabuuchi, T. Tani, Y. Kato, Y. Tsunoda, Journal of Experimental Zoology, 289, 208, 212,   2001年02月15日
    概要:The in vitro and in vivo developmental potential of nuclear transferred embryos receiving follicular epithelial cells pretreated with spermine (5 and 20 mM), protamine (0.25 and 25 mg/ml), or putrescine (1 and 100 μg/ml) at room and reduced temperatures was examined in the mouse. The pretreated donor cells were first fused with enucleated oocytes, and then nuclei from reconstituted eggs at the two-cell stage were fused with the enucleated fertilized two-cell embryos. The proportion of reconstituted embryos that developed into blastocysts was not significantly different among groups. After transfer to recipients, implantation rates were not different between groups and fetuses were obtained in protamine- and spermine-treated groups as well as in control groups. These results demonstrate that pretreatment of nuclear donor cells with spermine, protamine, or putrescine does not enhance the developmental potential in vitro or in vivo in the mouse. © 2001 Wiley-Liss, Inc.
  • Effects of seasonality and fasting on the plasma leptin and thyroxin levels of the raccoon dog (Nyctereutes procyonoides) and the blue fox (Alopex lagopus), Tomokazu Amano, Tetsuya Tani, Yoko Kato, Yukio Tsunoda, Yukio Tsunoda, Journal of Experimental Zoology, 289, 109, 118,   2001年02月01日
    概要:Plasma leptin and thyroxin concentrations of eleven raccoon dogs and eleven blue foxes were monitored for 6 months. Half of the animals were placed on a 3-week fast in November. Leptin levels were low in summer, but in October they rose significantly. In November, leptin concentrations decreased rapidly within a week although the body mass of the animals remained stable. There were no significant differences between experimental groups for raccoon dogs, but in blue foxes the fasting group had lower leptin levels than the control group. High thyroxin levels in summer decreased as autumn progressed, but thyroxin concentrations of the fasting groups increased at the end of the fast. Leptin levels of the raccoon dog and the blue fox are not determined only by the fat reserves of the animals, but they seem to reflect the autumnal deposition of fat at the onset of winter. Blue foxes have metabolic rates of active animals during the winter and higher leptin levels in December than raccoon dogs. The superficially hibernating raccoon dogs have low leptin levels after the onset of winter perhaps as an adaptation to fasting. J. Exp. Zool. 289:109-118, 2001. © 2001 Wiley-Liss, Inc.
  • Direct exposure of chromosomes to nonactivated ovum cytoplasm is effective for bovine somatic cell nucleus reprogramming, T. Tani, Y. Kato, Y. Tsunoda, Biology of Reproduction, 64, 324, 330,   2001年01月20日
    概要:We examined the in vitro developmental potential of non-activated and activated enucleated ova receiving cumulus cells at various stages of the cell cycle. Eleven to 29% of activated ova receiving donor cells stopped developing at the 8-cell stage but 21% to 50% of nonactivated ova receiving donor cells at either the G0, G1, G2, or M phase, or cycling cells developed into blastocysts. One normal calf was born after transferring five blastocysts that had developed from ova receiving donor cells at the M phase. The present study demonstrated that direct exposure of donor chromosomes to nonactivated ovum cytoplasm is effective for somatic cell nucleus reprogramming, and activated ovum cytoplasm does not reprogram the nucleus.
  • The recent progress on nuclear transfer in mammals, Yukio Tsunoda, Yoko Kato, Zoological Science, 17, 1177, 1184,   2000年12月01日
    概要:Differentiated mammalian somatic cell nuclei and embryonic nuclei can now be reprogrammed to develop into young if they are introduced into enucleated oocytes. The success rates for cloning are generally low, however, and peri-and postnatal death rates of the young are high. Cloning technology will be useful for the genetic improvement of farm animals, therapeutic human protein production, and organ or tissue transplantation into humans. In addition, the information obtained on nuclear reprogramming will be helpful for understanding the fundamental mechanisms of differentiation and aging.
  • Development of rabbit parthenogenetic oocytes and nuclear-transferred oocytes receiving cultured cumulus cells, X. J. Yin, T. Tani, Y. Kato, Y. Tsunoda, Theriogenology, 54, 1469, 1476,   2000年12月01日
    概要:The present study determined a suitable parthenogenetic activation procedure for rabbit oocytes and examined the developmental potential of enucleated oocytes receiving cultured cumulus cells. Unfertilized oocytes recovered from superovulated rabbits were activated with one or two sets of electrical pulses, with or without subsequent administration of 6-dimethylaminopurine (6-DMAP). The proportion of oocytes treated with one or two sets of electrical pulses and 6-DMAP that cleaved (87% and 98%, respectively) and developed into blastocysts (77% and 85%, respectively) was significantly higher (P < 0.05) than those activated with electrical pulses alone (30% and 42% for cleavage, 7% and 17% for blastocysts). Cumulus cells separated from ovulated oocytes obtained from mature rabbits were cultured for three to five passages and then induced to quiescence by serum starvation before nuclear transfer. The enucleated oocytes receiving cumulus cells were activated with electrical pulses followed by the addition of 6-DMAP, and cultured in vitro for 5 to 6 d or transferred to pseudopregnant recipient females 1 d after activation. Of 186 nuclear-transferred oocytes, 123 (66%) cleaved and 42 (23%) developed into blastocysts. After transfer of 174 nuclear-transferred oocytes to 8 recipient females, a total of 3 implantation sites were observed in 3 recipient females but no fetuses were obtained. © 2000 by Elsevier Science Inc.
  • Cloning of calves from various somatic cell types of male and female adult, newborn and fetal cows, Y. Kato, T. Tani, Y. Tsunoda, Journal of Reproduction and Fertility, 120, 231, 237,   2000年11月29日
    概要:Twenty-four calves were cloned from six somatic cell types of female and male adult, newborn and fetal cows. The clones were derived from female cumulus (n=3), oviduct (n=2) and uterine (n=2) cells, female and male skin cells (n=10), and male ear (n=5) and liver (n=2) cells. On the basis of the number of cloned embryos transferred (n=172) to surrogate cows, the overall rate of success was 14%, but based on the number of surrogate mothers that became pregnant (n=50), the success rate was 48%. Cell nuclei from uterus, ear and liver cells, which have not been tested previously, developed into newborn calves after nuclear transfer into enucleated oocytes. To date, seven female and six male calves have survived: six of the females were from adults cells (cumulus (n=3), oviduct (n=2) and skin (n=1) cells) and one was from newborn skin cells, whereas the male calves were derived from adult ear cells (n=3), newborn liver and skin cells (n=2), and fetal cells (n=1). Clones derived from adult cells frequently aborted in the later stages of pregnancy and calves developing to term showed a higher number of abnormalities than did those derived from newborn or fetal cells. The telomeric DNA lenghts in the ear cells of three male calves cloned from the ear cells of a bull aged 10 years were similar to those of the original bull. However, the telomeric DNA lengths from the white blood cells of the clones, although similar to those in an age-matched control, were shorter than those of the original bull, which indicates that telomeric shortening varies among tissues.
  • 【再生医学と生命科学】 生殖工学 体細胞核移植による動物クローニング, 角田 幸雄, 加藤 容子, 蛋白質・核酸・酵素, 45, 13, 2015, 2020,   2000年09月
    概要:動物クローニングの現状について概説した.従来,種々の発生ステージの初期胚やオタマジャクシの体細胞の核移植によって正常なカエルが得られていたが,成長したカエルの体細胞核からはカエルは得られていなかった.最近,6歳の雌ヒツジの乳腺の培養細胞から仔ヒツジが得られたことをきっかけに,哺乳類では成体から採取した卵丘細胞や様々な組織の体細胞を核移植して,個体が得られるようになった.しかしながら,分化した体細胞核が個体への発生能を獲得する分子機構は不明なままである
  • Developmental potential of cumulus cell-derived cultured cells frozen in a quiescent state after nucleus transfer, T. Tani, Y. Kato, Y. Tsunoda, Theriogenology, 53, 1623, 1629,   2000年05月01日
    概要:An efficient method for freezing donor cells is necessary when using nucleus transfer of somatic cells for large-scale cloning. In the present study, we developed a method for freezing and thawing bovine cumulus cell- derived cultured cells to be used as nucleus donors. Cumulus cells were obtained from ovaries of living and slaughtered bovine and cultured in vitro. Cumulus cell-derived cultured cells were serum-starved for several days to induce a quiescent state and then frozen at -70°C for at least 2 d. Immediately thereafter or 2 h after thawing, the cells were used as donor cells for nuclear transfer without additional in vitro culture. The fusion rate with recipient cytoplasts was not affected by the cumulus cell source (slaughtered or living) or time after thawing (0 and 2 h). The cleavage rate of frozen-thawed cumulus cell-derived cultured cells from slaughtered cows immediately after thawing (0 h) was highest (97%) and was significantly higher than that of controls (85%) or cells transferred 2 h after thawing (85%). There were no significant differences among any of the groups in the potential of the nuclear transfer embryos to develop into blastocysts (34 vs 44 and 44%, 39 vs 45 and 46%). Thus, storage of bovine cumulus cell-derived cultured cells in the quiescent state at -70°C is effective and might be useful and convenient for large-scale cloning. The maximum storage periods and developmental potential of embryos after such nucleus transfers requires further examination. (C) 2000 by Elsevier Science Inc.
  • 【新世紀医療をめざしてII-クローンと再生医学】 家畜における核移植, 加藤 容子, 角田 幸雄, 遺伝子医学, 4, 2, 269, 273,   2000年05月
  • Production of mice derived entirely from embryonic stem cells after injecting the cells into heat treated blastocysts, T. Amano, K. Nakamura, T. Tani, Y. Kato, Y. Tsunoda, Theriogenology, 53, 1449, 1458,   2000年04月15日
    概要:The sensitivity of the inner cell mass (ICM) and trophectoderm (TE) of mouse blastocysts to high temperatures was examined. When blastocysts with a diameter of 100 to 120 μm treated for 15 to 20 min at 45°C were cultured in vitro, the cell number in the ICM did not increase, although that in the TE did increase. After transfer of treated blastocysts to recipients, implantation was not drastically inhibited but no live fetuses were obtained. These results demonstrated that the ICM at the blastocyst stage was more sensitive to high temperature than the TE. ICM clumps or ES cells were injected into blastocysts treated for 20 min at 45°C. After transfer of injected blastocysts to recipients, we obtained mice derived completely from ICM or ES cells as judged by GPI analysis. Since 4 of 7 ES-cell derived mice, but none of the 6 mice derived from the ICM died after birth, an as yet unidentified epigenetic alteration might have occurred during the establishment and/or culture of ES cells.
  • Efficient cryopreservation of bovine blastocysts derived from nuclear transfer with somatic cells using partial dehydration and vitrification, B. X. Nguyen, B. X. Nguyen, Y. Sotomaru, T. Tani, Y. Kato, Y. Tsunoda, Theriogenology, 53, 1439, 1448,   2000年04月15日
    概要:Preservation by vitrification of Day 7 and Day 8 bovine blastocysts derived from nuclear transfer with cumulus cells was compared with preservation of in vitro fertilized blastocysts. In Experiment 1, embryos were vitrified in PBS containing 60% ethylene glycol. In Experiment 2, they were vitrified in combination with partial dehydration using a solution of 39% ethylene glycol + 0.7 M sucrose and 8.6% Ficoll. In Experiment I, survival and hatching rates were 44 and 95% for nuclear transferred embryos, and 78 and 55% for in vitro fertilized embryos, respectively. In Experiment 2, survival and hatching rates were 93 and 95% for nuclear transfer embryos, and 77 and 85% for in vitro fertilized embryos, respectively. It is concluded that Day 7 and Day 8 bovine blastocysts derived from cumulus cells could be cryopreserved without the loss of viability by a simple and efficient method using a combination of partial dehydration and vitrification. (C) 2000 by Elsevier Science Inc.
  • Animal cloning by somatic nuclear transfer, Y. Tsunoda, Y. Kato, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 45, 2015, 2020,   2000年01月01日
  • Developmental potential of mouse follicular epithelial cells and cumulus cells after nuclear transfer, Yoko Kato, Akiko Yabuuchi, Nami Motosugi, Jun Ya Kato, Yukio Tsunoda, Yukio Tsunoda, Biology of Reproduction, 61, 1110, 1114,   1999年10月
    概要:The developmental potential after nuclear transfer of mouse follicular epithelial cells cultured in vitro was examined. Follicular epithelial cells surrounding growing oocytes (type 5, diameter of oocytes, 62.6 ± 5.9 μm; n = 14) were obtained from ovaries of adult mice. Before nuclear transfer, cells were cultured for several passages and subjected to serum starvation for several days. When the nuclear-transfer oocytes were at the 2-cell stage, serial nuclear transfer was performed. Additionally, cumulus cells surrounding ovulated oocytes were used as nuclear donors, with or without thermal stimulation (from -25°C to 60°C for 10 min) before nuclear transfer. Nuclear-transfer oocytes with follicular epithelial cells developed into blastocysts (34%) after serial nuclear transfer, and 4 living fetuses on Day 10.5 (25%, 16 transferred) and 1 dead fetus on Day 19.5 of pregnancy (3%, 30 transferred) were obtained after transfer to recipients. Although blastocysts (20%) were obtained after serial nuclear transfer of cumulus cells, only one implantation site without a fetus was observed on Day 10.5 of pregnancy. Thermal stimulation of cumulus cells before nuclear transfer did not enhance the ability to develop into fetuses or blastocysts.
  • Induction of pluripotency by injection of mouse trophectoderm cell nuclei into blastocysts following transplantation into enucleated oocytes, Y. Sotomaru, Y. Kato, Y. Tsunoda, Theriogenology, 52, 213, 220,   1999年07月15日
    概要:Pluripotency of mouse trophectoderm (TE) cells was examined using a nuclear transfer technique. We transferred a TE cell to an enucleated oocyte and cultured the reconstituted oocyte to the blastocyst stage. Then a portion of the inner cell mass (ICM) isolated from the TE-origin blastocyst was injected into the cavity of a fertilized blastocyst to produce a chimeric embryo, which was transferred to a recipient female. Of 319 oocytes reconstituted with TE cells, 263 (82.4%) had a single nucleus (1PN), 3 (0.9%) had 2 nuclei (2PN) and 53 (16.6%) had a nucleus with a polar body (1PN1PB). Although the oocytes with 1PN and 2PN developed to blastocysts (81 of 263, 30.8% and 1 of 3, respectively), only those with 1PN were used to produce chimeric blastocysts. After the transfer of chimeric embryos to recipient females, 7 (28%) of 25 conceptuses analyzed at midgestation showed chimerism. Of those 5 (71%), 6 (86%) and 4 (57%) chimeric conceptuses showed distribution of donor nuclei in the fetus, membrane and placenta, and the distributions were 10 to 65, 10 to 50 and 10 to 15%, respectively. Of the 23 young obtained, 7 (30%; 2 males and 5 females) were coat color chimeras. The contributions of donor nuclei were detected in the brain, lung, heart, liver, kidney, testis, ovary and blood. Each coat-color chimeric mouse was mated with CD-1 male or female mice, but no germ line chimera was obtained. When ICM cells were used as the control nuclear donor, the contribution was equivalent to those of TE cells. In conclusion, pluripotency of mouse TE cells on a somatic line was induced, and chimeric young were obtained using a nuclear transplantation technique.
  • Developmental potential of mouse primordial germ cells, Yoko Kato, William M. Rideout, William M. Rideout, Kathy Hilton, Sheila C. Barton, Yukio Tsunoda, M. Azim Surani, Development, 126, 1823, 1832,   1999年05月
    概要:There are distinctive and characteristic genomic modifications in primordial germ cells that distinguish the germ cell lineage from somatic cells. These modifications include, genome-wide demethylation, erasure of allele-specific methylation associated with imprinted genes, and the re-activation of the X chromosome. The allele-specific differential methylation is involved in regulating the monoallelic expression, and thus the gene dosage, of imprinted genes, which underlies functional differences between parental genomes. However, when the imprints are erased in the germ line, the parental genomes acquire an equivalent epigenetic and functional state. Therefore, one of the reasons why primordial germ cells are unique is because this is the only time in mammals when the distinction between parental genomes ceases to exist. To test how the potentially imprint-free primordial germ cell nuclei affect embryonic development, we transplanted them into enucleated oocytes. Here we show that the reconstituted oocyte developed to day 9.5 of gestation, consistently as a small embryo and a characteristic abnormal placenta. The embryo proper also did not progress much further even when the inner cell mass was 'rescued' from the abnormal placenta by transfer into a tetraploid host blastocyst. We found that development of the experimental conceptus was affected, at least in part, by a lack of gametic imprints, as judged by DNA methylation and expression analysis of several imprinted genes. The evidence suggests that gametic imprints are essential for normal development, and that they can neither be initiated nor erased in mature oocytes; these properties are unique to the developing germ line.
  • Eight calves cloned from somatic cells of a single adult, Yoko Kato, Tetsuya Tani, Yusuke Sotomaru, Kazuo Kurokawa, Jun Ya Kato, Hiroshi Doguchi, Hiroshi Yasue, Yukio Tsunoda, Science, 282, 2095, 2098,   1998年12月11日
    概要:Eight calves were derived from differentiated cells of a single adult cow, five from cumulus cells and three from oviductal cells out of 10 embryos transferred to surrogate cows (80 percent success). All calves were visibly normal, but four died at or soon after birth from environmental abuses, and postmortem analysis revealed no abnormality. These results show that bovine cumulus and oviductal epithelial cells of the adult have the genetic content to direct the development of newborn calves.
  • Nuclear transplantation of mouse inner cell mass and trophectoderm cells into enucleated two-cell embryos, Yusuke Sotomaru, Yoko Kato, Yukio Tsunoda, Journal of Reproduction and Development, 44, 1, 6,   1998年12月01日
    概要:Trophectoderm (TE) and inner cell mass (ICM) cells from blastocytsts were compared for their ability to produce chimeric mice or conceptuses (pluripotency) using nuclear transplantation in which a single cell was transplanted into an enucleated blastomere of a 2-cell embryo. The developmental ability of the embryos reconstituted with TE and ICM cells was dependent on when the recipient 2-cell embryos were recovered after hCG administration. When 2-cell embryos obtained 38-42 and 43-46 h post hCG injection were used, cell division of the reconstituted embryos rarely progressed to the 4-cell stage. However, when embryos recovered 48-51 h post hCG were used, the proportion of cleaved embryos increased significantly, developing to blastocysts with several large blastomeres. When reconstituted embryos were treated with nocodazole after nuclear transplantation, the percentage of cleaved embryos significantly increased, with some developing to blastocysts. Such blastocysts were transferred to recipient females, and no donor nuclei were detected in the conceptuses at midgestation. The in vitro developmental ability of the chimeric 2cell embryos reconstituted with TE and ICM cells from blastocysts was quite limited and no chimeric conceptuses were obtained.
  • クローン動物はどのようにして作られるか, 角田 幸雄, 加藤 容子, 化学と生物, 化学と生物, 36, 9, 572, 577,   1998年09月25日
  • Production of Monozygotic Twins after Freezing and Thawing of Bisected Mouse Embryos, Yusuke Sotomaru, Yoko Kato, Yoko Kato, Yukio Tsunoda, Yukio Tsunoda, Cryobiology, 37, 139, 145,   1998年09月01日
    概要:We examined the viability of mouse bisected embryos after freezing and thawing and produced monozygotic twin mice from these embryos. Two-cell embryos were collected from superovulated mature agouti, F1 hybrid (C57BL/6 X CBA) female mice. For bisection, one blastomere of the embryo was aspirated with a micropipette and injected into an empty zona pellucida. After culture for 24 to 28 h to the compacted 4- to 8-cell stage or 48 to 52 h to the late morula to blastocyst stage, the embryos were slowly frozen (-0.5 to -1.0°C/min), thawed (30°C/min), cultured for 24 h, and then transferred to recipient females. The bisected embryos without zonae pellucidae had developmental ability in vitro similar to those with zonae pellucidae (88% vs 89%). However, after freezing and thawing at the compacted 4- to 8-cell stage, bisected embryos with zonae pellucidae had higher viability than those without (60% vs 15%). Zona enclosed, bisected embryos frozen at the compacted 4- to 8-cell stage were more resistnat to freezing and thawing than those at the late morula to blastocyte stage (60% vs 23%). After transfer to recipients 26% of the zona enclosed bisected embryos frozen-thawed at the 4- to 8-cell stage developed to living fetuses a day 17.5 to 18.0 of pregnancy, which was slightly but not significantly lower than that of fresh bisected embryos (48%). On the other hand, only 5% of bisected embryos frozen-thawed at the late morula to blastocyst stage developed to young. The transfer of 15 sets of twin blastocysts as pairs that had been frozen and thawed at the compacted 4- to 8-cell stage yielded 2 (13%) sets of monozygotic twins. © 1998 Academic Press.
  • Not only inner cell mass cell nuclei but also trophectoderm nuclei of mouse blastocysts have a developmental totipotency, Y. Tsunoda, Y. Kato, Journal of Reproduction and Fertility, 113, 181, 184,   1998年07月01日
    概要:The nuclei of mouse trophectoderm cells were found to have developmental totipotency like inner cell mass cells after serial nuclear transfer. Single inner cell mass or trophectoderm cells from expanded blastocysts synchronized with the cell cycle by treatment with nocodazole and aphidicolin to the G1 stage were injected into the perivitelline space of enucleated metaphase II oocytes together with Sendai virus. All oocytes were given three electrical pulses to induce fusion and activation (first nuclear transfer). Aphidicolin was present in all media used until fusion. When reconstituted oocytes developed to the two-cell stage, the nuclei of the reconstituted eggs were fused with the enucleated blastomeres of fertilized two-cell embryos by inactivated Sendai virus (second nuclear transfer). The reconstituted embryos were cultured in vitro and transferred to recipients. After the second nuclear transfer, 23-64% (for inner cell mass cells) and 32-62% (for trophectoderm cells) developed to morula or blastocyst stage. Better development of second nuclear transfer embryos was observed when oocytes fused with trophectoderm nuclei did not extrude a polar body after the first nuclear transfer. After transfer of morulae and blastocysts to recipients, four males were obtained, two from inner cell mass and two from trophectoderm nuclei. These findings indicate that the nucleus of inner cell mass and trophectoderm cells of mouse blastocysts can be reprogrammed within the cytoplasm of unfertilized oocytes and then in fertilized embryos.
  • Developmental potential of embryonic and somatic cells in mammals, Y. Kato, Y. Tsunoda, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 43, 397, 404,   1998年01月01日
  • 体細胞の核移植 : クローン動物作出研究の将来展望, 角田 幸雄, 加藤 容子, The Journal of reproduction and development, The Journal of reproduction and development, 43, 6, j123, j126,   1997年12月01日
  • マウス生殖系列上細胞核の多能性に関する研究, 加藤 容子, The Journal of reproduction and development, The Journal of reproduction and development, 43, 6, j47, j54,   1997年12月01日
    概要:In the present study, the pluripotency of embryonic cells on the germ line, especially fetal germ cells, was examined. The results obtained are as follows; when donor cells on the germ line were aggregated with precompacted stage embryos or injected into blastocysts, fertile chimeric mice or fetuses were obtained from 3.5, 4.5 and 5.5 days cells and ES-cells, respectively. However, further stage cells never contributed to the conceptuses even though analyzed at 10.5 days of pregnancy. When fetal germ cells on days 14.5-16.5 were reprogrammed by fusion with enucleated oocytes and then treated with fertilized embryos, they can form chimeric fetuses and extraembryonic tissues. It was suggested that differentiated cells on the germ line such as fetal germ cells can also develop to chimeric fetuses after reprogramming. However, it was also clear that they died at the midgestation probably due to the inappropriate imprinting.
  • Effects of the age of donor embryos on the developmental ability of bovine nuclear transferred eggs in vitro, Katsuhiro Ohkoshi, Masaki Hata, Yoko Kato, Yukio Tsunoda, Journal of Reproduction and Development, 43, 261, 265,   1997年12月
    概要:The effect of the age of a donor embryo on the developmental ability of bovine nuclear transferred eggs was examined. A blastomere of an in vitro fertilized embryo cultured for 3.5 to 6.5 days, or an inner cell mass cell (ICM) from a blastocyst cultured for 7.5 days was fused with an enucleated, preactivated oocyte matured in vitro by electrical stimulation. Fused eggs were cocultured with cumulus cells for 10 days in vitro. The fusion rate of blastomeres from day 3.5 to 6.5 embryos with enucleated oocytes (78 to 88%) and the developmental ability of nuclear transferred eggs to blastocysts (6 to 10%) were not significantly affected by the age of the donor embryo. However, the fusion rate of the ICM cell was significantly low (28%). Although nuclear transferred eggs receiving an ICM cell developed to 2-cell (73%) and 8-cell (22%) stages at the same rates as those obtained in other groups (68 to 81% for 2-cell, 30 to 34% for 8-cell), none of them developed to blastocysts.
  • Effects of several factors on the monozygotic twin production in the mouse, Minkang Wang, Yoko Kato, Yukio Tsunoda, Journal of Reproduction and Development, 43, 91, 95,   1997年12月, 査読有り
    概要:Monozygotic mouse twins were produced by micromanipulation of 2-cell stage embryos and factors affecting the embryonic development were examined. In experiment 1, 2-cell embryos obtained from superovulated CD-1 strain or Fl (C57BLJ6 x CBA) females mated with CD-1 strain males were separated into a single blastomere. Pairs of twin blastomeres were cultured with or without supplementation of growth factors, a mixture of IGF-1, TGF-β and EGF for 3 days and twin blastocysts developed were transferred to recipient females. Of 174 pairs of Fl embryos, 88 to 100% developed to twin blastocysts. In contrast, development of CD-1 embryos (835 pairs) was significantly lower (25 to 36%). Of 63 Fl twin blastocysts transferred, 7 to 29% developed to twin fetuses, whereas only 0 to 8% of 62 pairs of CD-1 embryos developed to twins. In experiment 2, one nucleus of a 2-cell stage Fl embryo was electrically fused with an enucleated recipient 2-cell embryo. The 2-cell embryo from which a nucleus was removed was cultured together with the reconstituted embryo as a monozygotic twin pairs. Of 24 and 28 twin pairs cultured with or without growth factors, 92 and 86% developed to twin blastocysts, respectively. After transfer of 13 twin blastocysts to recipients, 3 and 2 twin fetuses were obtained. These findings indicated that monozygotic twin mice can be produced more efficiently from Fl embryos than from CD-1 embryos. Methods of removal of zona pellucida and addition of growth factors to the culture medium had no effect on the efficiency of production of monozygotic twins.
  • Developmental ability of CD-1 strain mouse embryos in vitro and in vivo with the different glucose phosphate isomerase patterns, Yoko Kato, Miho Tanimura, Yukio Tsunoda, Journal of Reproduction and Development, 43, 205, 211,   1997年12月, 査読有り
    概要:Glucose phosphate isomerase (GPI)-IAA and GPI-1BB homozygotes were produced by natural mating of CD-1 strain mice and the several reproductive characteristics of both homozygotes were compared. The pregnancy rate, period required for copulation and litter size were essentially the same. The developmental ability of GPI-1AA zygotes and 2-cell embryos in vitro was significantly lower than that of GPI-1BB (60 vs 75% and 75 vs 98%, respectively). After preservation at 4C for 24 h, the proportion of the GPI-1 AA-2-cell embryos developed to blastocysts was significantly low (28 vs 57%). After transfer of GPI-1AA embryos to recipient mice, the developmental potential into fetus was similar or significantly superior to that of GPI-1BB (19 vs 24% and 47 vs 14%, respectively). When both embryos were aggregated at the 8-16-cell stage and transferred to recipients, 76% of the young (13/17) was chimeras and both isozymes types were observed in most tissues analyzed. In conclusion, both GPI-1AA and GPI-1BB embryos of the CD-1 strain mice were equally useful for researches in developmental biology and biotechnology, their features being basically the same.
  • A comparative investigation on the potency of cells from the inner cell mass and trophectoderm of mouse blastocysts to produce chimeras, Y. Sotomaru, Y. Kato, Y. Tsunoda, Y. Tsunoda, Theriogenology, 48, 977, 984,   1997年10月15日
    概要:The ability of trophectoderm (TE) cells to produce chimeric mice (pluripotency) was compared with that of inner cell mass (ICM) cells. TE and ICM cells of blastocysts and hatching or hatched blastocysts derived from albino mice (CD-1, Gpi-1a/a) were aggregated with zona cut 8- to 16-cell stage embryos or injected into the blastocoele from non-albino mice (C57BL/6 x C3H/He, Gpi-1b/b). After transfer to pseudopregnant female mice, the contribution of the donor cells was examined by glucose phosphate isomerase (GPI) analysis of embryos, membrane and placenta at mid-gestation (Day 10.5 and 12.5) or by the coat color of newborn mice. In contrast to ICM cells, there was no contribution of TE cells in the conceptuses and no coat color chimeric young were obtained. After pre-labeling of TE cells with fluorescent latex microparticles, they were aggregated with embryos and the allocation of TE cells at the compacted morula and blastocyst stages was observed under a fluorescent microscope. Although the TE cells were observed attached onto the surface of the embryos at morula and blastocyst stages, unlike the ICM cells, they were not positively incorporated into the embryos. Thus, the pluripotency of TE cells from mouse blastocysts was not induced by the aggregation and injection methods.
  • 哺乳類における胚操作技術の変遷 (シンポジウム 哺乳類におけるクロ-ン研究の現状と今後の展望), 角田 幸男, 加藤 容子, 畜産の研究, 畜産の研究, 51, 10, 1094, 1098,   1997年10月
  • クローン家畜作出の研究動向, 角田 幸雄, 加藤 容子, 日本畜産學會報 = The Japanese journal of zootechnical science, 日本畜産學會報 = The Japanese journal of zootechnical science, 68, 6, 596, 602,   1997年06月25日
  • 凍結融解ウシ除核未受精卵への核移植, 大越 勝広, 秦 正樹, 加藤 容子, 角田 幸雄, 日本畜産學會報 = The Japanese journal of zootechnical science, 日本畜産學會報 = The Japanese journal of zootechnical science, 68, 1, 7, 12,   1997年01月25日
    概要:The in vitro developmental ability of frozen-thawed enucleated oocytes receiving a donor blastomere of embryos fertilized in vitro was studied. All recipient oocytes were enucleated before freezing and were activated 9 hours before fusion with a donor blastomere from 8-to 20-cell stage embryos. The enucleated oocytes were frozen in 1.0 or 1.5M glycerol by conventional slow cooling methods. The developmental ability of oocytes was slightly higher when they were frozen before rather than after activation (in experiment 1). The developmental ability to 2-cell and 8-cell stage was high (70% and 26%, respectively) when aged oocytes (32 hours of maturation at activation) were used, but the development to blastocyst was only observed in young oocytes (23.5 hours) receiving a donor blastomere (2% in experiment 2). Although the developmental ability to 8-cell stage of frozen-thawed nuclear transferred oocytes was low (15%), a small ratio of them developed to blastocysts (2%).
  • Effects of the the age of donor embryos on the developmental ability of bovine nuclear transferred eggs in vitro, Ohkoshi,K., Hata,M., Kato,Y., Tsunoda,Y. , J.Reprod.Dev 261-265, 1997., 43, 261, 265,   1997年, 査読有り
  • ウシ過齢単為発生卵の発生能が低い原因に関する一考察, 高野 博, 小財 千明, 清水 悟, 加藤 容子, 角田 幸雄, 日本畜産學會報 = The Japanese journal of zootechnical science, 日本畜産學會報 = The Japanese journal of zootechnical science, 67, 11, 991, 995,   1996年11月25日
    概要:Effect of aging of oocytes on the in vitro developmental ability of bovine parthenogenetic eggs were examined. The oocytes with a first polar body were activated at 24 (young) or 44 hours (aged) after starting of in vitro maturational culture by electric stimulation, then they were incubated in the medium supplemented with cytochalasin B and cycloheximide (CY) for 6 hours. After incubation, they were cultured with cumulus cells for 8 days in vitro. Karyoplast and cytoplast were separated from young and aged oocytes, respectively. Then, 4 combinations of reconstituted oocytes were produced, activated and cultured in vitro. A blastomere from in vitro fertilized 32 to 64 stage embryos was fused with enucleated young or aged cytoplast, and reconstituted oocytes were cultured in vitro. Results obtained were as follows. (1) The activation rate of young oocytes significantly increased after CY treatment (19% vs 97%). (2) The developmental ability of aged parthenogenetic eggs was significantly low compared with that of young oocytes. (3) Experiments on the exchang of karyoplast and cytoplast between young and aged oocytes, and nuclear transfer revealed that the low developmental ability of aged parthenogenetic oocytes was due to the developmental deficiency of chromosomes and cytoplasmic factor (s).
  • ブタ単為発生卵の体外培養条件の検討, 秦 正樹, 大越 勝広, 加藤 容子, 角田 幸雄, 日本不妊学会雑誌 = Japanese journal of fertility and sterility, 日本不妊学会雑誌 = Japanese journal of fertility and sterility, 41, 3, 77, 82,   1996年07月01日
  • ウシ核移植卵の体外発生能に及ぼすβ-メルカプトエタノールの影響, 大越 勝広, 新田 良平, 高野 博, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 12, 2, 127, 130,   1995年10月01日
  • Pluripotency of mouse embryonic cells on germ line at 3.5 to 8.5 and 11.5 days post coitum after aggregation with precompacted embryos., Kato,Y. and Tsunoda,Y.,, Develop.Growth & Differ., 37, 79, 84,   1995年, 査読有り
  • Germ cell nuclei of male fetal mice can support development of chimeras to midgestation following serial transplantation. , Kato,Y., Tsunoda,Y, Development, 121, 779, 783,   1995年, 査読有り
  • Nuclear transfer of inner cell mass cells and fetal germ cells at a different cell cycle into enucleated zygotes at M-phase in the mouse., Kato,Y. and Tsunoda,Y., J.Reprod.Dev.,, 41, 345, 351,   1995年, 査読有り
  • High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene, Nakane.H., Takeuchi.S., Yuba.S., Saijo.M., Nakatsu.Y., Murai.H., Nakatsuru.Y., Ishikawa.T., Hirota.S., Kitamura.Y., Kato.Y., Tsunoda.Y., Miyauchi.H., Horio.T., Tokunaga.T., Matsunaga.T., Nikaido.O., Nishimune.Y., Okada.Y., Tanaka.K., , Nature., 377, 165-168, 1995., 377, 165, 168,   1995年, 査読有り
  • Effects of the culture density of mouse zygotes on the development in vitro and in vivo. , Kato,Y. and Tsunoda,Y., Theriogenology, 41, 1315, 1322,   1994年, 査読有り
  • Effects of the reduction of cytoplasm of mouse 2-cell embryos on blastocoele formation timing and developmental ability in vitro and in vivo., Yoko Kato, Yukio Tsunoda, Theriogenology, 41, 1483, 1488,   1994年, 査読有り
  • Totipotency and pluripotency of embryonic nuclei in the mouse. , Kato,Y and Tsunoda,Y.,, Mol.Reprod.Develop.,, 36, 276, 278,   1993年, 査読有り
  • Nuclear transplantation of embryonic stem cells in mice, Yukio Tsunoda, Yoko Kato, J. Reprod. Fert., 98, 537, 540,   1993年, 査読有り
  • Cytogenetic analysis of reconstituted one-cell mouse embryos derived from nuclear transfer of fetal male germ cells, Tsunoda,Y., Kato,Y. and O'Neill,G.T., J.Reprod.Fert., 96, 275, 281,   1992年, 査読有り
  • Nuclear transplantation of mouse fetal germ cells into enucleated 2-cell embryos., Yoko Kato, Yukio Tsunoda, Theriogenology, 37, 769, 778,   1992年, 査読有り
  • Several factors affecting the nuclear formation after nuclear transfer of male fetal germ cells into enucleated eggs in the mouse, Kato,Y. and Tsunoda,Y.,, Jpn.J.Fertil.Steril., , 37, 375, 379,   1992年, 査読有り
  • Synchronous division of mouse 2-cell embryos with nocodazole in vitro., Yoko Kato, Yukio Tsunoda, J.Reprod.Fert., , 95, 39, 43,   1992年, 査読有り
  • 胎子期のマウス生殖細胞と初期胚との集合によるキメラ胚の発生に関する検討., 加藤容子 角田幸雄, Jpn.J.Anim.Reprod., 37, 225, 230,   1991年, 査読有り
  • マウス始原生殖細胞の採取,同定ならびに生存性に関する検討.J, 加藤容子・杉山恭子・角田幸雄,, Jpn.J.Anim.Reprod., 36, 235, 239,   1990年, 査読有り
  • Low temperature preservation of mouse fetal germ cells at 4 ℃., Kato,Y. Tsunoda,Y.,, Theriogenology, 45, 1029, 1035,   1996年, 査読有り

書籍等出版物

  • Principles of cloning, Cibelli Jose B., Academic Press, an imprint of Elsevier,   2014年, 9780123865410
  • Transgenic animal technology : a laboratory handbook, Pinkert Carl A., Elsevier,   2014年, 9780124104907
  • 科学技術者のための実践生命倫理, 角田 幸雄, 昭和堂,   2012年02月, 4812212073
  • クローニングの原理 第13章 細胞の由来が核移植効率に及ぼす影響, 角田 幸雄, 加藤 容子, 共著, Academic Press,   2002年10月
    概要:ドナー細胞の由来が、核移植卵の発生能に及ぼす影響について紹介した。(英文) (分担執筆)
  • トランスジェニック動物作出技術 第7章 核移植, 角田 幸雄, 加藤 容子, 共著, Academic Press,   2002年11月
    概要:トランスジェニック動物作出マニュアルの中で、核移植技術について紹介した。(英文)(分担執筆)
  • Encyclopedia of Dairy Sciences, 和訳)クローニング, 加藤 容子, 角田 幸雄, 共著, Academic Press(U. K.),   2002年12月
    概要:哺乳動物のクローニングについて解説した。章名Gamete and embryo technology(分担執筆)

講演・口頭発表等

  • 体細胞クローンマウス卵子のDNAメチレーションエラー, 吉岡 匠、神長 祐子、大畠 一輝、加藤 容子、小池 佐、小林 久人、河野 友宏, 第109回日本繁殖生物学会,   2016年09月
  • Evaluation of the impact of pre-exposure to PHA on developmental efficiency of porcine PA and SCNT embryos, 第121回日本畜産学会,   2016年03月
  • マウス個体の老化が生殖能力に及ぼす影響ならびに個体老化の緩和に関する予備的検討, 山口 正義, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   2015年09月
  • ブタ精子の前処理がICSI卵の体外発生能に及ぼす影響, 秦 仁樹, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   2015年09月
  • Oct3/4,Nanog遺伝子の発現を指標としたマウス体細胞核移植胚の移植前選別の試み, 大畠一輝、谷哲弥、加藤容子, 第108回日本繁殖生物学会,   2015年
  • マウス体細胞核移植胚の発生能と胚盤胞期におけるNanogの発現様式との関連性, 大畠一輝、谷哲弥、加藤容子, 第56回日本卵子学会,   2015年
  • マウス体細胞核移植卵の第一卵割における不等分裂改善の試み, 大畠 一輝, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   2014年08月
  • 体外加齢ブタ未受精卵の生物学的特性と体外発生能の検討, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   2014年08月
  • 核移植研究の動向と課題, 加藤容子, 第55回日本卵子学会,   2014年05月18日, 招待有り
  • マウス体細胞核移植胚の2細胞期における割球サイズの差異が発生能に及ぼす影響, 大畠 一輝, 谷 哲弥, 加藤 容子, Journal of Mammalian Ova Research,   2014年04月
  • 融解後のMG132処理がウシ凍結-融解未受精卵の体外発生能に及ぼす影響, 清水 聡一郎, 谷 哲弥, 加藤 容子, Journal of Mammalian Ova Research,   2014年04月
  • 核移植研究の動向と課題, 加藤 容子, Journal of Mammalian Ova Research,   2014年04月
  • 過剰排卵処置の有無がマウス体細胞核移植卵の発生能に及ぼす影響, 松下 淳, 谷 哲弥, 加藤 容子, 日本畜産学会大会講演要旨集,   2014年03月
  • マウス体細胞核移植卵の第一卵割における不等分裂改善の試み, 大畠一輝、谷哲弥、加藤容子, 第107回日本繁殖生物学会,   2014年
  • 体外加齢ブタ未受精卵の生物学的特性と体外発生能の検討, 谷哲弥、加藤容子, 第107回日本繁殖生物学会,   2014年
  • マウス体細胞核移植胚の2細胞期における割球サイズの差異が発生能に及ぼす影響, 大畠一輝、谷哲弥、加藤容子, 第55回日本卵子学会,   2014年
  • 融解後のMG132処理がウシ凍結-融解未受精卵の体外発生能に及ぼす影響, 清水聡一郎、谷 哲弥、加藤容子, 第55回日本卵子学会,   2014年
  • 受胚雌へのPlacental Lactogen(PL)投与がマウスクローン胚の体内発生能に及ぼす影響, 松下 淳, 加藤 容子, The Journal of Reproduction and Development,   2013年08月
  • ブタ未受精卵の体外加齢抑制法の最適化, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   2013年08月
  • マウス体細胞核移植胚の移植前選別の試み, 大畠 一輝, 加藤 容子, The Journal of Reproduction and Development,   2013年08月
  • マウスにおける体内受精卵および体細胞核移植胚由来一卵性双子胚の遺伝子発現, 大畠 一輝, 加藤 容子, Journal of Mammalian Ova Research,   2013年04月
  • 受胚雌へのプロラクチンの投与がマウス体細胞核移植卵の体内発生能に及ぼす影響, 松下 淳, 加藤 容子, Journal of Mammalian Ova Research,   2013年04月
  • マウス体細胞核移植由来2細胞期分離胚の培養条件の検討, 大畠 一輝, 加藤 容子, 日本畜産学会大会講演要旨集,   2013年03月
  • 受胚雌へのPlacental Lactogen(PL)投与がマウスクローン胚の体内発生能に及ぼす影響, 松下 淳、加藤容子, 第106回日本繁殖生物学会,   2013年
  • ブタ未受精卵の体外加齢抑制法の最適化, 谷哲弥、加藤容子, 第106回日本繁殖生物学会,   2013年
  • マウス体細胞核移植胚の移植前選別の試み、第106回日本繁殖生物学会, 大畠一輝、加藤容子, 第106回日本繁殖生物学会,   2013年
  • マウスにおける体内受精卵および体細胞核移植胚由来一卵性双子胚の遺伝子発現, 大畠一輝、加藤容子, 第54回日本卵子学会,   2013年
  • 受胚雌へのプロラクチンの投与がマウス体細胞核移植卵の体内発生能に及ぼす影響, 松下 淳、加藤容子, 第54回日本卵子学会,   2013年
  • マウス体細胞核移植由来2細胞期分離胚の透明帯への再挿入が分離胚の発生能に及ぼす影響, 大畠 一輝, 角田 幸雄, 加藤 容子, The Journal of Reproduction and Development,   2012年08月
  • ブタ卵子におけるMII期保存の試み, 辻 暖永, 角田 幸雄, 加藤 容子, The Journal of Reproduction and Development,   2012年08月
  • 核リプログラミング因子強発現卵による核リプログラミング能増強の試み, 谷 哲弥, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2012年08月
  • マイクロマニピュレーション技術とクローン研究, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2012年04月01日
  • マウス核移植由来2細胞期分離胚の体外発生能, 大畠 一輝, 角田 幸雄, 加藤 容子, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2012年04月01日
  • ブタ単為発生卵ならびに体細胞核移植卵の体外発生能に及ぼすグルタミン濃度の影響, 辻 暖永, 角田 幸雄, 加藤 容子, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2012年04月01日
  • マニピュレーションと発生研究 マイクロマニピュレーション技術とクローン研究, 加藤 容子, 角田 幸雄, Journal of Mammalian Ova Research,   2012年04月
  • ラット体細胞核移植卵における第一卵割のタイミングとその後の体外発生能との関係性, 水本 茂利, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2011年08月
  • マウス受精卵の発生能に及ぼす生薬抽出物の影響, 杉本 貴章, 辻 優大, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2011年04月01日
  • 低温処理によるラット卵子の可逆的な自然活性化阻害の試み, 水本 茂利, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2011年04月01日
  • メラトニンがブタ体細胞核移植卵の体外発生能に及ぼす影響, 中野 真夕, 谷 哲弥, 加藤 容子, 福田 愛作, 森本 義晴, 角田 幸雄, 日本IVF学会誌,   2010年09月
  • ドナーとして用いる卵丘細胞の培養がラット体細胞核移植卵の体外発生能に及ぼす影響, 水本 茂利, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2010年08月
  • 単為発生卵との共移植がマウス体細胞核移植(SCNT)胚の発生能に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2010年08月
  • KSOMを用いたラット体細胞核移植卵の培養, 水本 茂利, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2010年04月01日
  • 受精卵との共移植がマウス体細胞核移植胚の体内発生能に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2010年04月01日
  • 受胚雌へのProgesterone投与がマウス体細胞核移植卵の発生能に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2009年08月
  • 培養細胞を用いたラット体細胞核移植, 水本 茂利, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2009年08月
  • メラトニンがブタ単為発生卵ならびに体細胞核移植卵の体外発生能に及ぼす影響, 中野 真夕, 谷 哲弥, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2009年04月01日
  • 受胚雌へのhCG投与がマウス体細胞核移植胚の発生能に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2009年04月01日
  • ブタ体細胞核移植卵の体外発生能に及ぼすバブプロ酸の影響, 谷 哲弥, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2009年03月
  • 活性化付与のタイミングがラット体細胞核移植卵の前核形成および体外発生能に及ぼす影響, 水本 茂利, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2009年03月
  • Cyclosporin Aの投与が体細胞核移植卵の体内発生能に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, Reproductive Immunology and Biology,   2008年11月
  • Cyclosporin Aの投与が受精卵移植後の胎子形成に及ぼす影響, 辻 優大, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2008年08月
  • ラット体細胞核移植卵における紡錘体の継時的観察と体外発生能の検討, 水本 茂利, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2008年08月
  • トリコスタチンA(TSA)がラット体細胞核移植卵の体外発生能に及ぼす影響, 水本 茂利, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2008年04月01日
  • マウス体細胞核移植卵の発生能に及ぼすTSAならびに5-azadCの影響, 辻 優大, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2008年04月01日
  • 体細胞クローン胚の作出と選別, 加藤 容子, The Journal of reproduction and development,   2007年09月01日
  • ウシ未受精卵からの初期化因子の同定と体細胞核移植への利用, 谷 哲弥, 島田 浩明, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2007年09月
  • ラット単為発生卵におけるMG132とdemecolcineの影響, 水本 茂利, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2007年09月
  • 母体への胚移植時期が体細胞核移植胚の胎子への発生能に及ぼす影響, 川又 美弥子, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2007年04月01日
  • マウス体細胞核移植卵の発生能に及ぼすレシピエント卵の aging の影響, LIU Guohui, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2007年04月01日
  • ウシ体細胞核移植卵の初期化におけるMAPKの必要性, 谷 哲弥, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2006年08月
  • ウシ核移植卵母細胞の最初の卵割の時期(The timing of the first cleavage of bovine nuclear-transferred oocytes), Amarnath Dasari, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2006年03月
  • クローン胚盤胞中の形態学及びmRNA発現パターンに関する比較研究(Comparative studies on the morphology and mRNA expression pattern in cloned blastocysts), Li Xiangping, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2006年03月
  • ウシ体細胞核移植卵のスピンドルチェックポイント, 谷 哲弥, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2005年08月
  • クローン化ウシ胚盤胞における発生関連遺伝子発現の比較(Comparison of development-related gene expression in cloned bovine blastocysts), Li Xiangping, Amarnath Dasari, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2005年03月
  • 体細胞クローン個体作出研究の現状と問題点, 角田 幸雄, 加藤 容子, 日本畜産学会大会講演要旨集,   2005年03月
  • マウス桑実胚割球の核移植, 八津川 彰吾, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2004年04月01日
  • 減数分裂の再開を抑制したブタ卵子の体外発生能 ; 単為発生誘起ならびに体細胞核移植法を用いた検討, 川上 雅弘, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2004年04月01日
  • 核移植方法の違いがブタ体細胞核移植卵の体外発生能に及ぼす影響, 河野 康二郎, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2004年03月
  • 再生医学のがん治療への応用 ES細胞からin vitroで蠕動運動する腸管をつくる, 山田 高嗣, 久永 倫聖, 金広 裕道, 高木 都, 鳥橋 茂子, 加藤 容子, 角田 幸雄, 中島 祥介, 日本癌治療学会誌,   2002年09月
  • マウスES細胞の核移植;核移植方法,細胞の遺伝的背景ならびに細胞周期の影響, 薮内 晶子, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2002年03月
  • マウス ES 細胞ならびに排卵卵丘細胞の核移植, 角田 幸雄, 薮内晶子, 加藤 容子, 第 99 回日本畜産学会 (長野),   2001年09月, 第 99 回日本畜産学会 (長野)
    概要:核移植卵の発生能に及ぼす再核置換ならびに集合の影響について
  • 活性化条件とドナー細胞の種類が豚体細胞由来再構築卵の体外発生能に及ぼす影響, 角田 幸雄, Yin Xi Jun, 谷 哲弥, 川上雅弘, 加藤 容子, 米村功, 第 99 回日本畜産学会 (長野),   2001年09月, 第 99 回日本畜産学会 (長野)
    概要:ブタ体細胞核移植卵の 10%が胎盤胞へ発生することを明らかにした。
  • ブタ核移植卵の体外発生能に及ぼすドナー細胞及び核移植卵の培養気相条件の影響, 角田 幸雄, 川上雅弘, 谷 哲弥, Yin Xi Jun, 加藤 容子, 第 100 回日本畜産学会 (東京),   2002年03月, 第 100 回日本畜産学会 (東京)
    概要:培養中の酸素濃度の影響について検討した。
  • マウス ES 細胞の核移植;核移植方法、 細胞の遺伝的背景並びに細胞周期の影響, 角田 幸雄, 薮内晶子, 加藤 容子, 第 100 回日本畜産学会 (東京),   2002年03月, 第 100 回日本畜産学会 (東京)
    概要:核移植方法、 ES 細胞を樹立したマウスの遺伝的背景並びに細胞周期の違いがマウス ES 細胞由来核移植卵の体外並びに産子の発生能に及ぼす影響について検討した。
  • 胚細胞、体細胞の核移植, 加藤 容子, 角田 幸雄, XIX International Congress of Genetics, Symposium on Stem Cells and Development(オーストラリア),   2003年07月, XIX International Congress of Genetics, Symposium on Stem Cells and Development(オーストラリア)
    概要:胚細胞、体細胞の核移植について情報を提供した。
  • 家畜の核移植, 加藤 容子, 角田 幸雄, 第18 回日本生殖免疫学会学術集会シンポジウム(山口),   2003年11月, 第18 回日本生殖免疫学会学術集会シンポジウム(山口)
    概要:家畜の核移植に関する情報を提供した。
  • マウスES細胞ならびに排卵卵丘細胞の核移植;再核置換及び集合の影響, 薮内 晶子, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2001年08月
  • マウスES細胞の核移植:産子生産率が低い原因に関する一考案, 天野 朋和, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2001年03月
  • マウスES細胞の核移植によるクローンマウスの作出, 天野 朋和, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2000年12月
  • ドナー細胞及びレシピエント卵細胞質の細胞周期の組み合わせがウシ卵丘細胞由来核移植卵の体外発生能に及ぼす影響, 谷 哲弥, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2000年12月
  • 体外成熟ウサギ卵子をレシピエントにした体細胞の核移植, Yin Xi Jun, 谷 哲弥, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   2000年12月
  • 高温処理胚盤胞への注入によるES細胞由来マウスの作出 特に4倍体胚盤胞との比較に関する検討, 天野 朋和, 谷 哲弥, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2000年03月
  • マウス排卵卵丘細胞由来核移植卵の体外発生能の検討, 薮内 晶子, 谷 哲弥, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   2000年03月
  • 体細胞クローン動物, 角田 幸雄, 谷 哲弥, 加藤 容子, The Journal of Reproduction and Development,   1999年12月
  • 輸送した卵子或いは卵巣由来のレシピエント卵子がウシ卵丘細胞核移植卵の体外発生能に及ぼす影響, 谷 哲弥, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   1999年12月
  • 高温処理胚盤胞への注入によるES細胞由来マウスの作出, 天野 朋和, 谷 哲弥, 加藤 容子, 角田 幸雄, 日本畜産学会大会講演要旨集,   1999年09月
  • 体細胞クローン動物, 角田 幸雄, 谷 哲弥, 加藤 容子, 日本内分泌学会雑誌,   1999年09月
  • Spermine、Protamine ならびに Putrescine 処理がマウス卵胞細胞の核移植に及ぼす影響, 薮内 晶子, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1999年04月01日
  • マウス肝臓由来細胞を用いた核移植, 本杉 奈美, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1999年04月01日
  • 高温処理胚盤胞への注入による内細胞塊(ICM)細胞由来個体の作出, 天野 朋和, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1999年04月01日
  • ウシ卵丘細胞由来核移植卵の発生能に及ぼすドナー細胞の凍結保存的影響, 谷 哲弥, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1999年04月01日
  • クローン研究の最前線, 角田 幸雄, 加藤 容子, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1999年04月01日
  • 牛及びマウスの体細胞を用いた核移植の現状と問題点, 角田 幸雄, 加藤 容子, 日本獣医学会学術集会講演要旨集,   1999年03月
  • 継代培養ウシ卵丘細胞の核移植,特にドナー細胞の細胞周期同調法の違いが核移植卵の発生能に及ぼす影響, 谷 哲弥, 外丸 祐介, 加藤 順也, 加藤 容子, 角田 幸雄, The Journal of Reproduction and Development,   1998年12月
  • 凍結/融解した二分割胚からの双子マウスの作出, 外丸 祐介, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1998年04月01日
  • ウシ卵丘細胞の核移植, 特にドナー細胞の低血清処理日数の影響, 谷 哲弥, 外丸 祐介, 加藤 順也, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1998年04月01日
  • ウシ核移植卵の発生能に及ぼす性腺刺激ホルモン, 成長因子およびシステアミンの影響, 大越 勝広, 加藤 容子, 角田 幸雄, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   1997年04月01日
  • 継代されたES細胞のキメラ形成能と集合胚における分布, 加藤 容子, 角田 幸雄, 哺乳動物卵子学会誌 = Journal of Mammalian Ova Research,   1995年04月01日

MISC

  • マウス2細胞期胚の低温保存, 加藤 容子, 詫摩 美里, 角田 幸雄, 詫摩 美里, タクマ ミサト, Takuma Misato, 角田 幸雄, ツノダ ユキオ, Tsunoda Yukio, 近畿大学農学部紀要, 27, 27, 79, 84,   1994年03月15日, http://ci.nii.ac.jp/naid/110000978629
    概要:本研究では,低温感作を与えたマウス卵管が初期胚の低温保存容器として適しているとのArav and Shehuの報告を追試することを目的に実施した。過剰排卵処理を施したCD-1系雌マウスを同系雄と交配し,(1)hCG投与後20および45時間目に卵巣付近-20℃の綿花で2分間冷却したしたのち卵管を摘出し,低温保存,(2)感作を与えていないマウスの卵管を保存,(3)感作を与えていないマウスの卵管から回収した2細胞期胚をマイクロチューブ内に移して保存した。各区とも4℃で24〜120時間保存後,回収した胚を体外培養に併し,胚盤胞への発生能と細胞数を検討した。胚盤胞への発生能は保存48時間までは各区で大差がみとめられなかったが,72時間保存すると,(1)区の発生率は(2)区あるいは(3)区に比べて有意に高かった。しかしながら,96および120時間保存胚ではいずれの区の発生率も低く,低温感性による胚の低温保存に対する保護作用はみとめられなかった。胚盤胞期の細胞数は(1)区では保存時間に関わりなく差はみられなかったが,(2)あるいは(3)区では新鮮胚を培養したときと比べて細胞数は有意に減少した。以上の結果から,予め低温感作を与えたマウス卵管内で胚を低温保存しても,感作を与えない卵管内での保存あるいは胚単独で保存した場合と比べて,胚の発生能は大きく向上せず,Arav and Shehuの実験結果を再現するには至らなかった。

特許

  • オウレンおよびオウバク抽出物あるいはベルベリンまたはその塩を含有する避妊薬ならびにそれらを用いた受胎調節法, 角田 幸雄, 加藤 容子, 特願2009-245547, 特開2011-088874
  • 体細胞核初期化因子, 角田 幸雄, 加藤 容子, 谷 哲弥, 特願2002-329772, 特開2004-161682
  • 体細胞核初期化因子, 角田 幸雄, 加藤 容子, 谷 哲弥, 特願2002-329772, 特開2004-161682, 特許第3736517号
  • 化学的染色体除去法による核移植用レシピエント卵の調製方法およびそれを用いるクローン豚の作出方法, 角田 幸雄, 加藤 容子, 谷 哲弥, 特願2001-330815, 特開2003-125673
  • 細胞核移植方法およびそれを用いるクローン哺乳動物の生産方法, 角田 幸雄, 加藤 容子, 谷 哲弥, 特願2000-000082, 特開2001-186827
  • 体細胞より発生したウシ初期胚由来継代細胞株の樹立法, 角田 幸雄, 加藤 容子, 谷 哲弥, 特願2000-000080, 特開2001-186876

競争的資金

  • JSPS外国人招聘研究者, (長期), ブタ卵子(胚)を用いた新しいポータブル培 養システムの開発, 加藤容子
  • JSPS科学研究費助成事業, 挑戦的萌芽研究, 哺乳動物初期胚の凍結乾燥保存の試み, 加藤容子
  • JSPS外国人特別研究員事業, ブタ幹細胞と初期化関連因子を用いたクローニングの効率化に関する研究, 加藤容子
  • 文科省, 特定領域研究「生殖系列」, 天然物を利用した体細胞核の初期化促進に関する研究, 加藤容子
  • 文科省, 特定領域研究「幹細胞」, 哺乳動物未受精卵に含まれる初期化因子の探索, 加藤容子
  • 文科省, 特定領域研究「発生システム」, 細胞核の初期化に関する研究, 加藤容子
  • JSPS科学研究費助成事業, 萌芽的研究, 核移植を介さない新しい個体作出法の開発, 加藤容子
  • JSPS科学研究費助成事業, 基盤研究B, 体細胞を用いた新しい個体作出法に関する基礎的研究, 加藤容子

教育活動情報

担当経験のある科目

  • 動物分子発生学特論, 近畿大学
  • 動物発生工学特論, 近畿大学
  • 動物生産学, 近畿大学
  • 実験動物学, 近畿大学
  • 動物発生工学, 近畿大学
  • 生命科学基礎, 近畿大学