松橋 珠子 (マツハシ タマコ)

  • 先端技術総合研究所 講師
Last Updated :2024/03/24

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    和牛の産肉能力に影響を与える遺伝子やたんぱく質について研究しています。また、地方自治体で肉用牛の改良と振興に携わったことから、現場への普及にも重きを置いています。
  • 報道関連出演・掲載一覧

    <報道関連出演・掲載一覧> ●2019/3/7 産経新聞 夕刊 和牛の管理体制について ●2019/3/7 産経新聞 和牛受精卵の中国への不正持ち出しについて

研究者情報

学位

  • 博士(獣医学)(東京大学)

ホームページURL

J-Global ID

研究キーワード

  • 分子遺伝学   野生動物   黒毛和種   家畜繁殖学   分子生物学   畜産学   

現在の研究分野(キーワード)

    和牛の産肉能力に影響を与える遺伝子やたんぱく質について研究しています。また、地方自治体で肉用牛の改良と振興に携わったことから、現場への普及にも重きを置いています。

研究分野

  • ライフサイエンス / 動物生産科学
  • ライフサイエンス / 分子生物学

経歴

  • 2016年04月 - 現在  現職
  • 2015年04月 - 2016年03月  岐阜県農政部
  • 2006年04月 - 2015年03月  岐阜県畜産研究所
  • 2001年04月 - 2004年03月  日本学術振興会特別研究員(DC1)

学歴

  •         - 2006年03月   東京大学   農学生命科学研究科
  •         - 1999年03月   北海道大学   地球環境科学研究科
  •         - 1997年03月   北海道大学   農学部

所属学協会

  • 日本哺乳類学会   日本RNAi研究会・日本細胞外小胞学会   日本プロテオーム学会   日本繁殖生物学会   肉用牛研究会   日本動物遺伝育種学会   関西畜産学会   日本畜産学会   

研究活動情報

論文

  • 血中エクソソームに内包された牛枝肉成績バイオマーカーマイクロRNAの探索
    松橋 珠子; 笠原 喜斗; 加藤 亜惟; 茶原 裕佳; 池上 春香; 村瀬 華梨; 白木 琢磨; 松本 和也
    日本獣医学会学術集会講演要旨集 164回 [HLO - 35] (公社)日本獣医学会 2021年09月
  • Fuki Kawaguchi; Miyako Tsuchimura; Kenji Oyama; Tamako Matsuhashi; Shin Maruyama; Hideyuki Mannen; Shinji Sasazaki
    Archives Animal Breeding 63 1 9 - 17 2020年01月 [査読有り]
     
    Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.
  • 松橋 珠子
    関西畜産学会報 = Kansai journal of animal science 176 1 - 8 関西畜産学会 2019年03月 [招待有り]
  • 松橋珠子
    食肉に関する助成研究調査成果報告書 36 166‐171  2018年11月
  • 松橋珠子
    近畿大学先端技術総合研究所紀要 23 31‐36  2018年03月 [査読有り]
  • 黒毛和種去勢牛の脂肪交雑を生体評価する バイオマーカー候補タンパク質の血清プロテオーム解析による探索
    池上春香; 松橋珠子; 永井宏平; 宮本 圭; 大林賢伍; 坂口慎一; 松本和也
    関西畜産学会報 174 1 - 10 2018年03月 [査読有り]
  • Takashi Hirano; Tamako Matsuhashi; Kenji Takeda; Hiromi Hara; Naohiko Kobayashi; Kazuo Kita; Yoshikazu Sugimoto; Kei Hanzawa
    ANIMAL SCIENCE JOURNAL 87 9 1178 - 1181 2016年09月 [査読有り]
     
    Isoleucyl-tRNA synthetase (IARS) c.235G>C (p.V79L) is a causative mutation for a recessive disease called IARS disorder in Japanese black cattle. The disease is involved in weak calf syndrome and is characterized by low birth weight, weakness and poor suckling. The gestation period is often slightly extended, implying that intrauterine growth is retarded. In a previous analysis of 2597 artificial insemination (AI) procedures, we suggested that the IARS mutation might contribute toward an increase in the incidence of prenatal death. In this study, we extended this analysis to better clarify the association between the IARS mutation and prenatal death. The IARS genotypes of 92 animals resulting from crosses between carrier (G/C) x G/C were 27 normal (G/G), 55G/C and 10 affected animals (C/C) (expected numbers: 23, 46 and 23, respectively). Compared to the expected numbers, there were significantly fewer affected animals in this population (P<0.05), suggesting that more than half of the affected embryos died prenatally. When the number of AI procedures examined was increased to 11580, the frequency of re-insemination after G/C x G/C insemination was significantly higher at 61-140 days (P<0.001). The findings suggested that the homozygous IARS mutation not only causes calf death, but also embryonic or fetal death.
  • Takahiro Nishimaki; Takayuki Ibi; Siqintuya; Naohiko Kobayashi; Tamako Matsuhashi; Takayuki Akiyama; Emi Yoshida; Kazumi Imai; Mayu Matsui; Keiichi Uemura; Hisayoshi Eto; Naoto Watanabe; Tatsuo Fujita; Yosuke Saito; Tomohiko Komatsu; Hiroshi Hoshiba; Hideyuki Mannen; Shinji Sasazaki; Tetsuo Kunieda
    ANIMAL SCIENCE JOURNAL 87 4 469 - 476 2016年04月 [査読有り]
     
    Marker-assisted selection (MAS) is expected to accelerate the genetic improvement of Japanese Black cattle. However, verification of the effects of the genes for MAS in different subpopulations is required prior to the application of MAS. In this study, we investigated the allelic frequencies and genotypic effects for carcass traits of six genes, which can be used in MAS, in eight local subpopulations. These genes are SCD, FASN and SREBP1, which are associated with the fatty acid composition of meat, and NCAPG, MC1R and F11, which are associated with carcass weight, coat color and blood coagulation abnormality, respectively. The frequencies of desirable alleles of SCD and FASN were relatively high and that of NCAPG was relatively low, and NCAPG was significantly associated with several carcass traits, including carcass weight. The proportions of genotypic variance explained by NCAPG to phenotypic variance were 4.83 for carcass weight. We thus confirmed that NCAPG is a useful marker for selection of carcass traits in these subpopulations. In addition, we found that the desirable alleles of six genes showed no negative effects on carcass traits. Therefore, selection using these genes to improve target traits should not have negative impacts on carcass traits.
  • 丸山新; 勝田智博; 西和隆; 松橋珠子; 山田英信; 向島幸司
    肉用牛研究会報 100 34‐35  2016年01月
  • Shinji Sasaki; Takayuki Ibi; Tamako Matsuhashi; Kenji Takeda; Shogo Ikeda; Mayumi Sugimoto; Yoshikazu Sugimoto
    BMC GENETICS 16 1 123  2015年10月 [査読有り]
     
    Background: Female fertility, a fundamental trait required for animal reproduction, has gradually declined in the last 2 decades in Japanese Black cattle. To identify associated genetic variants in Japanese Black cattle, we evaluated female fertility as a metric to describe the average inverse of the number of artificial inseminations required for conception from the first through the fourth parity (ANAI(4)) and conducted a genome-wide association study (GWAS) using 430 animals with extreme ANAI(4) values from 10,399 animals. Results: We found that 2 variants, namely a single-nucleotide polymorphisms (SNP; g.48476925C > T) and a 3-bp indel (g.48476943_48476946insGGC), in the upstream region of the activin receptor IIA gene (ACVR2A) were associated with ANAI(4). ACVR2A transcripts from Japanese Black cattle of the Q haplotype, defined by the SNP and the 3-bp indel, with increased ANAI(4) were 1.29-1.32-fold more abundant than q-derived transcripts. In agreement, reporter assay results revealed that the activity of the ACVR2A promoter was higher in reporter constructs with the Q haplotype than in those with the q haplotype by approximately 1.2 fold. Expression of exogenous ACVR2A induced dose-dependent increases of reporter activity from the follicle-stimulating hormone, beta polypeptide (FSHB) promoter in response to activin A in a pituitary gonadotrophic cell line. The findings suggested that sequence variations in the upstream region of ACVR2A with the Q haplotype increased ACVR2A transcription, which in turn induced FSHB expression. This association was replicated using a sample population size of 1,433 animals; the frequency of the Q haplotype was 0.39, and Q-to-q haplotype substitution resulted in an increase of 0.02 in terms of ANAI(4). Conclusions: This GWAS identified variants in the upstream region of ACVR2A, which were associated with female fertility in Japanese Black cattle. The variants affected the level of ACVR2A mRNA expression, which could lead to an allelic imbalance. This association was replicated with a sample population of 1,433 animals. Thus, the results suggest that the Q haplotype could serve as a useful marker to select Japanese Black cattle with superior female fertility.
  • Akiko Takasuga; Kunio Sato; Ryouichi Nakamura; Yosuke Saito; Shinji Sasaki; Takehito Tsuji; Akio Suzuki; Hiroshi Kobayashi; Tamako Matsuhashi; Koji Setoguchi; Hiroshi Okabe; Toshitake Ootsubo; Ichiro Tabuchi; Tatsuo Fujita; Naoto Watanabe; Takashi Hirano; Shota Nishimura; Toshio Watanabe; Makio Hayakawa; Yoshikazu Sugimoto; Takatoshi Kojima
    PLOS GENETICS 11 8 e1005433  2015年08月 [査読有り]
     
    Recessive skeletal dysplasia, characterized by joint- and/or hip bone-enlargement, was mapped within the critical region for a major quantitative trait locus (QTL) influencing carcass weight; previously named CW-3 in Japanese Black cattle. The risk allele was on the same chromosome as the Q allele that increases carcass weight. Phenotypic characterization revealed that the risk allele causes disproportional tall stature and bone size that increases carcass weight in heterozygous individuals but causes disproportionately narrow chest width in homozygotes. A non-synonymous variant of FGD3 was identified as a positional candidate quantitative trait nucleotide (QTN) and the corresponding mutant protein showed reduced activity as a guanine nucleotide exchange factor for Cdc42. FGD3 is expressed in the growth plate cartilage of femurs from bovine and mouse. Thus, loss of FDG3 activity may lead to subsequent loss of Cdc42 function. This would be consistent with the columnar disorganization of proliferating chondrocytes in chondrocyte-specific inactivated Cdc42 mutant mice. This is the first report showing association of FGD3 with skeletal dysplasia.
  • Kiri Hayakawa; Takayuki Sakamoto; Atsushi Ishii; Keita Yamaji; Yoshinobu Uemoto; Nanae Sasago; Eiji Kobayashi; Naohiko Kobayashi; Tamako Matsuhashi; Shin Maruyama; Hirokazu Matsumoto; Kenji Oyama; Hideyuki Mannen; Shinji Sasazaki
    ANIMAL SCIENCE JOURNAL 86 8 737 - 746 2015年08月 [査読有り]
     
    The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
  • 池上春香; 永井宏平; 松橋珠子; 小林直彦; 武本淳史; 吉廣卓哉; 井上悦子; 樋口智香; 守田昂太郎; 内堀翔; 天野朋子; 田口善智; 加藤博己; 入谷明; 松本和也
    日本畜産学会報 86 2 141 - 152 Japanese Society of Animal Science 2015年06月 [査読有り]
     
    黒毛和種肥育牛の枝肉形質を推定するバイオマーカー候補タンパク質の同定を目的に,枝肉形質情報ならびに腎周囲白色脂肪組織のプロテオーム解析情報を搭載した統合情報管理システムを運用し,プロテオーム解析データを持つ去勢牛200頭から,5つの形質(枝肉重量・ロース芯面積・バラの厚さ・皮下脂肪の厚さ・BMSナンバー)に関して上位と下位の2群を選抜して,この2群間で314個のタンパク質スポットの発現量と枝肉成績との関連性を検討した.各形質の上位群(平均値+標準偏差)および下位群(平均値−標準偏差)として抽出した個体間の各スポットのタンパク質発現量を比較した結果,合計でタンパク質45種類(90スポット)の発現量に有意な差が認められた.これらタンパク質の一部について,代謝経路における位置付けを行なうとともに,vimentinのウエスタンブロット解析より発現量を検証したところ,枝肉形質を推定するバイオマーカー候補タンパク質としての可能性が示唆された.
  • 松橋 珠子
    動物遺伝育種研究 42 2 71 - 77 Japanese Society of Animal Breeding and Genetics 2014年12月 [招待有り]
  • 渡辺 大作; 谷地村 結未; 武田 祐典; 野村 徹; 富岡 美千子; 平野 貴; 松橋 珠子; 杉本 喜憲
    産業動物臨床医学雑誌 5 2 137 - 139 日本家畜臨床学会 2014年11月
  • 永井 宏平; 池上 春香; 松橋 珠子; 武本 淳史; 南方 佑介; 樋口 智香; 守田 昂太郎; 小林 直彦; 松本 和也
    日本プロテオーム学会大会要旨集 2014 98 - 98 日本プロテオーム学会(日本ヒトプロテオーム機構) 2014年
  • Atsushi Ishii; Keita Yamaji; Yoshinobu Uemoto; Nanae Sasago; Eiji Kobayashi; Naohiko Kobayashi; Tamako Matsuhashi; Shin Maruyama; Hirokazu Matsumoto; Shinji Sasazaki; Hideyuki Mannen
    ANIMAL SCIENCE JOURNAL 84 10 675 - 682 2013年10月 [査読有り]
     
    Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome-wide association study with 50K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40657 SNPs were used in this study. We applied genome-wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl-CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition.
  • 小林 栄治; 奥村 直彦; 湊 和之; 黒木 政博; 安田 康明; 新居 雅宏; 松橋 珠子
    日本養豚学会誌 = The Japanese journal of swine science 50 3 137 - 141 日本養豚学会 2013年09月 [査読有り]
     
    ミトコンドリアDNA(mtDNA)は,母性遺伝すること,変異率が高いことおよびその変異が蓄積することなどから,集団遺伝学的解析に広く用いられている(CASTROら,1998; BOORE,1999)。ブタにおけるmtDNAの解析から,その塩基配列により大きくヨーロッパ型とアジア型に分類され,ニホンイノシシはリュウキュウイノシシとはやや異なるものの,同じアジア型に分類されている(OKUMURAら,1996;2001; WATANOBEら,1999)。一方,イノシシにおける核ゲノムDNAの分析は,ブタの家畜化起源の研究の中で,幾つかの毛色関連遺伝子等が分析されている(GIUFFRAら,2000; LARSONら,2005;2010)。特に,ニホンイノシシでは,第1番染色体上のGPIP遺伝子(Glucosephosphate isomerase-processed pseudogene)がよく調べられている(ISHIGUROら,2002;2005;2008)。しかしながら,GPIP遺伝子においては欧米品種を分析した報告が多くないことおよびほとんどの変異が塩基配列の決定によることなどから,分析の効率化が必要である。成長ホルモン(Growth hormone,GH)遺伝子は,第12番染色体上に位置し,ブタの成長に関与すると期待される遺伝子であり,大別するとヨーロッパ型,アジア型および共通型の3種に分類されている(小林ら,1999)。そこで本研究では,豚遺伝子の流入状況を調査するための基礎情報として,2000年時点のニホンイノシシについて集団遺伝学的調査を行った。また,mtDNAを簡易に分析するため,ミスマッチプライマーを用いたPCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism)法によるヨーロッパ型またはアジア型の判定法を確立し,合わせて分析した。
  • Takahiro Nishimaki; Takayuki Ibi; Yoshihiro Tanabe; Yoshiyuki Miyazaki; Naohiko Kobayashi; Tamako Matsuhashi; Takayuki Akiyama; Emi Yoshida; Kazumi Imai; Mayu Matsui; Keiichi Uemura; Naoto Watanabe; Tatsuo Fujita; Yosuke Saito; Tomohiko Komatsu; Takahisa Yamada; Hideyuki Mannen; Shinji Sasazaki; Tetsuo Kunieda
    ANIMAL SCIENCE JOURNAL 84 8 585 - 591 2013年08月 [査読有り]
     
    Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise F-ST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies.
  • Takashi Hirano; Naohiko Kobayashi; Tamako Matsuhashi; Daisaku Watanabe; Toshio Watanabe; Akiko Takasuga; Mayumi Sugimoto; Yoshikazu Sugimoto
    PLoS ONE 8 5 e64036  2013年05月 [査読有り]
     
    We identified an IARS (isoleucyl-tRNA synthetase) c.235G> C (p.Val79Leu) substitution as the causative mutation for neonatal weakness with intrauterine growth retardation (perinatal weak calf syndrome). In Japanese Black cattle, the syndrome was frequently found in calves sired by Bull A. Hence, we employed homozygosity mapping and linkage analysis. In order to identify the perinatal weak calf syndrome locus in a 4.04-Mb region of BTA 8, we analysed a paternal half-sibling family with a BovineSNP50 BeadChip and microsatellites. In this critical region, we performed exome sequencing to identify a causative mutation. Three variants were detected as possible candidates for causative mutations that were predicted to disrupt the protein function, including a G> C (p.Val79Leu) mutation in IARS c.235. The IARS c.235G> C mutation was not a homozygous risk allele in the 36 healthy offspring of Bull A. Moreover, the IARS Val79 residue and its flanking regions were evolutionarily and highly conserved. The IARS mutant (Leu79) had decreased aminoacylation activity. Additionally, the homozygous mutation was not found in any of 1526 healthy cattle. Therefore, we concluded that the IARS c.235G> C mutation was the cause of hereditary perinatal weak calf syndrome. © 2013 Hirano et al.
  • Shinji Sasaki; Takayuki Ibi; Toshio Watanabe; Tamako Matsuhashi; Shogo Ikeda; Yoshikazu Sugimoto
    BMC GENETICS 14 14 41  2013年05月 [査読有り]
     
    Background: Calving efficiency can be described as the measure of a cow's ability to produce viable offspring within a specific period of time. This trait is crucial in beef cattle because calves are necessary both for the production of beef and for heifer replacements. Recently, the number of calves produced at 4 years of age (NCP4) has been used to evaluate the calving efficiency of Japanese Black cattle. To identify variants associated with calving efficiency in Japanese Black cattle, we conducted a genome-wide association study (GWAS) using 688 animals with extreme NCP4 values selected from 15,225 animals. Results: We identified genetic variants on bovine chromosome 12 (BTA12) that were associated with NCP4. The General Transcription Factor IIF, polypeptide 2 (GTF2F2), located in the 132 kbp-associated region, proved to be in strong linkage disequilibrium. We found 15 associated variants in the promoter and the 3' UTR regions. Consistent with this finding, transcripts of GTF2F2 derived from the haplotype (Q) with the increased number of calves were 1.33-fold more abundant than q-derived transcripts. Furthermore, luciferase assays revealed that the activity of the 3' UTR, a region that includes nine SNPs, was higher in constructs with the Q haplotype than in those with the q haplotype by approximately 1.35-fold. In contrast, the activity of the promoter region did not differ between haplotypes. The association was replicated in an independent sample of 827 animals that were randomly selected from the remainder of the cohort from the same farms used in the GWAS. In the replicated population, the frequency of the Q haplotype is 0.313, and this haplotype accounts for 2.69% of the total phenotypic variance. The effect of the Q to q haplotype substitution on NCP4 was 0.054 calves. These findings suggest that variants in the 3' UTR of GTF2F2 affect the level of GTF2F2 mRNA, which is associated with calving efficiency. Conclusions: This GWAS has identified variants in the 3' UTR of GTF2F2 that were associated with the NCP4 of Japanese Black cattle, and this association was validated in an independent sample. The Q haplotype will be immediately useful in improving the calving efficiency of Japanese Black cattle.
  • T. Miyasaka; S. -N. Takeshima; M. Jimba; Y. Matsumoto; N. Kobayashi; T. Matsuhashi; H. Sentsui; Y. Aida
    TISSUE ANTIGENS 81 2 72 - 82 2013年02月 [査読有り]
     
    Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Bovine leukocyte antigen (BoLA) is strongly involved in the subclinical progression of BLV infections. Recent studies show that the BoLA-DRB3 gene might play a direct role in controlling the number of BLV-infected peripheral B lymphocytes in vivo in Holstein cattle. However, the specific BoLA class II allele and DRB3-DQA1 haplotypes determining the BLV proviral load in Japanese Black cattle are yet to be identified. In this study, we focused on the association of BLV proviral load and polymorphism of BoLA class II in Japanese Black cattle. We genotyped 186 BLV-infected, clinically normal cattle for BoLA-DRB3 and BoLA-DQA1 using a polymerase chain reaction-sequence-based typing method. BoLA-DRB3*0902 and BoLA-DRB3*1101 were associated with a low proviral load (LPVL), and BoLA-DRB3*1601 was associated with a high proviral load (HPVL). Furthermore, BoLA-DQA1*0204 and BoLA-DQA1*10012 were related to LPVL and HPVL, respectively. Furthermore, we confirmed the correlation between the DRB3-DQA1 haplotype and BLV proviral load. Two haplotypes, namely 0902B or C (DRB3*0902-DQA1*0204) and 1101A (DRB3*1101-DQA1*10011), were associated with a low BLV proviral load, whereas one haplotype 1601B (DRB3*1601-DQA1*10012) was associated with a high BLV proviral load. We conclude that resistance is a dominant trait and susceptibility is a recessive trait. Additionally, resistant alleles were common between Japanese Black and Holstein cattle, and susceptible alleles differed. This is the first report to identify an association between the DRB3-DQA1 haplotype and variations in BLV proviral load.
  • Mayuko Jimba; Shin-nosuke Takeshima; Hironobu Murakami; Junko Kohara; Naohiko Kobayashi; Tamako Matsuhashi; Takashi Ohmori; Tetsuo Nunoya; Yoko Aida
    BMC VETERINARY RESEARCH 8 8 167  2012年09月 [査読有り]
     
    Background: Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. Recently, we developed a new quantitative real-time polymerase chain reaction (PCR) method using Coordination of Common Motifs (CoCoMo) primers to measure the proviral load of known and novel BLV variants in BLV-infected animals. Indeed, the assay was highly effective in detecting BLV in cattle from a range of international locations. This assay enabled us to demonstrate that proviral load correlates not only with BLV infection capacity as assessed by syncytium formation, but also with BLV disease progression. In this study, we compared the sensitivity of our BLV-CoCoMo-qPCR method for detecting BLV proviruses with the sensitivities of two real-time PCR systems, and also determined the differences of proviral load with serotests. Results: BLV-CoCoMo-qPCR was found to be highly sensitive when compared with the real-time PCR-based TaqMan MGB assay developed by Lew et al. and the commercial TaKaRa cycleave PCR system. The BLV copy number determined by BLV-CoCoMo-qPCR was only partially correlated with the positive rate for anti-BLV antibody as determined by the enzyme-linked immunosorbent assay, passive hemagglutination reaction, or agar gel immunodiffusion. This result indicates that, although serotests are widely used for the diagnosis of BLV infection, it is difficult to detect BLV infection with confidence by using serological tests alone. Two cattle were experimentally infected with BLV. The kinetics of the provirus did not precisely correlate with the change in anti-BLV antibody production. Moreover, both reactions were different in cattle that carried different bovine leukocyte antigen (BoLA)-DRB3 genotypes. Conclusions: Our results suggest that the quantitative measurement of proviral load by BLV-CoCoMo-qPCR is useful tool for evaluating the progression of BLV-induced disease. BLV-CoCoMo-qPCR allows us to monitor the spread of BLV infection in different viewpoint compared with classical serotest.
  • 池上春香; 小林直彦; 松橋珠子; 武本淳史; 吉廣卓哉; 井上悦子; 加藤里恵; 加藤博己; 田口善智; 天野 朋子; 森本康一; 中川優; 入谷明; 松本和也
    日本畜産学会報 83 3 281 - 290 2012年08月 [査読有り]
  • T. Hirano; T. Matsuhashi; N. Kobayashi; T. Watanabe; Y. Sugimoto
    ANIMAL GENETICS 43 1 11 - 17 2012年02月 [査読有り]
     
    Mutations in the gene encoding fibrillin-1 (FBN1), a component of the extracellular microfibril, cause Marfan syndrome (MFS). Frequent observation of cattle with a normal withers height, but lower body weight than age-matched normal cattle, was recently reported among cattle sired by phenotypically normal Bull A, in Japanese Black cattle. These cattle also showed other characteristic features similar to the clinical phenotype of human MFS, such as a long phalanx proximalis, oval face and crystalline lens cloudiness. We first screened a paternal half-sib family comprising 36 affected and 10 normal offspring of Bull A using the BovineSNP50 BeadChip (illumina). Twenty-two microsatellite markers mapped to a significant region on BTA10 were subsequently genotyped on the family. The bovine Marfan syndrome-like disease (MFSL) was mapped onto BTA10. As FBN1 is located in the significant region, FBN1 was sequenced in Bull A, and three affected and one normal cattle. A G>A mutation at the intron64 splicing accepter site (c.8227-1G>A) was detected in 31 of 36 affected animals (84.7%). The c.8227-1G>A polymorphism was not found in 20 normal offspring of Bull A or in 93 normal cattle unrelated to Bull A. The mutation caused a 1-base shift of the intron64 splicing accepter site to the 3' direction, and a 1-base deletion in processed mRNA. This 1-base deletion creates a premature termination codon, and a 125-amino acid shorter Fibrillin-1 protein is produced from the mutant mRNA. We therefore conclude that the c.8227-1G>A mutation is causative for MFSL. Furthermore, it was suggested that Bull A exhibited germline mosaicism for the mutation, and that the frequency of the mutant sperm was 14.9%.
  • 池上 春香; 松橋 珠子; 武本 淳史; 永井 宏平; 樋口 智香; 守田 昂太郎; 小林 直彦; 松本 和也
    日本プロテオーム学会大会要旨集 2012 93 - 93 日本プロテオーム学会(日本ヒトプロテオーム機構) 2012年
  • Jimba M; Takeshima SN; Matsumoto Y; Kobayashi N; Matsuhashi T; Kohara J; Aida Y
    Retrovirology 8 A21  2011年06月 [査読有り]
  • Taku Miyasaka; Shin-nosuke Takeshima; Yuki Matsumoto; Naohiko Kobayashi; Tamako Matsuhashi; Yoshiyuki Miyazaki; Yoshihiro Tanabe; Kazuki Ishibashi; Hiroshi Sentsui; Yoko Aida
    GENE 472 1-2 42 - 49 2011年02月 [査読有り]
     
    In cattle, bovine leukocyte antigens (BoLAs) have been extensively used as markers for bovine diseases and immunological traits. In this study, we sequenced alleles of the BoLA class II loci, BoLA-DRB3 and BoLA-DQA1, from 650 Japanese cattle from six herds [three herds (507 animals) of Japanese Black cattle and three herds (143 animals) of Holstein cattle] using polymerase chain reaction-sequence-based typing (PCR-SBT) methods. We identified 26 previously reported distinct DRB3 alleles in the two populations: 22 in Japanese Black and 17 in Holstein. The number of DRB3 alleles detected in each herd ranged from 9 to 20. Next, we identified 15 previously reported distinct DQA1 alleles: 13 in Japanese Black and 10 in Holstein. The number of alleles in each herd ranged from 6 to 10. Thus, allelic divergence is significantly greater for DRB3 than for DQA1. A population tree on the basis of the frequencies of the DRB3 and DQA1 alleles showed that, although the genetic distance differed significantly between the two cattle breeds, it was closely related within the three herds of each breed. In addition, Wu-Kabat variability analysis indicated that the DRB3 gene was more polymorphic than the DQA1 gene in both breeds and in all herds, and that the majority of the hypervariable positions within both loci corresponded to pocket-forming residues. The DRB3 and DQA1 heterozygosity for both breeds within each herd were calculated based on the Hardy-Weinberg equilibrium. Only one Japanese Black herd showed a significant difference between the expected and observed heterozygosity at both loci. This is the first report presenting a detailed study of the allelic distribution of BoLA-DRB3 and -DQA1 genes in Japanese Black and Holstein cattle from different farms in Japan. These results may help to develop improved livestock breeding strategies in the future. (C) 2010 Elsevier B.V. All rights reserved.
  • T. Matsuhashi; S. Maruyama; Y. Uemoto; N. Kobayashi; H. Mannen; T. Abe; S. Sakaguchi; E. Kobayashi
    JOURNAL OF ANIMAL SCIENCE 89 1 12 - 22 2011年01月 [査読有り]
     
    The quality of fat is an important factor in defining the quality of meat. Fat quality is determined by the composition of fatty acids. Among lipid metabolism-related genes, including fatty acid synthesis genes, several genetic variations have been reported in the bovine fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD), sterol regulatory element-binding protein 1 (SREBP1), and GH genes. In the present study, we evaluated the single and epistatic effects of 5 genetic variations (4 SNP and 1 insertion/deletion) in 4 genes (FASN, SCD, SREBP1, and GH) on the fatty acid composition of the longissimus thoracis muscle and carcass and meat quality traits in 480 commercial Japanese Black cattle. Significant single effects of FASN, SCD, and GH(L127V) polymorphisms on the fatty acid composition of the longissimus thoracis muscle were detected. The A293V polymorphism of SCD had the largest effect on myristic acid (C14:0, P < 0.001), myristoleic acid (C14:1, P < 0.001), stearic acid (C18:0, P < 0.001), oleic acid (C18:1, P < 0.001), and MUFA (P < 0.001). Polymorphisms in the FASN, SCD, and SREBP1 genes showed no effect on any meat yield trait. There were no significant epistatic effects on fatty acid composition among pairs of the 3 genes (FASN, SCD, and SREBP1) involved in fatty acid synthesis. No epistatic interactions (P > 0.1) were detected between FASN and SCD for any carcass trait. When the genotypes of 3 markers (FASN, SCD, and GH(L127V)) were substituted from the lesser effect allele to the greater effect allele, the proportion of C18:1 increased by 4.46%. More than 20% of the genetic variance in the C18: 1 level could be accounted for by these 3 genetic markers. The present results revealed that polymorphisms in 2 fatty acid synthesis genes (FASN and SCD) independently influenced fatty acid composition in the longissimus thoracis muscle. These results suggest that SNP in the FASN and SCD genes are useful markers for the improvement of fatty acid composition in commercial Japanese Black cattle.
  • 牛白血病ウイルス(BLV)ウイルスロードとMHCクラスII遺伝子の相関性
    宮坂 卓; 竹嶋 伸之輔; 神馬 繭子; 松本 有生; 小林 直彦; 松橋 珠子; 泉對 博; 間 陽子
    日本獣医学会学術集会講演要旨集 147回 249 - 249 (公社)日本獣医学会 2009年03月
  • Tamako Matsuhashi; Tomohiko Akiyama; Fugaku Aoki; Senkiti Sakai
    ANIMAL SCIENCE JOURNAL 78 6 575 - 586 2007年12月 [査読有り]
     
    Gene expression in the implanting blastocyst is altered by stimulation with estrogen secreted from maternal ovaries. In the present study, to understand the mechanism regulating the changes in gene expression, diverse histone modifications in blastocysts were studied using a delayed implantation model, in which embryos were kept in a dormant state in the uterus by maternal ovariectomy and progesterone treatment, and then activated by injection with estrogen. Total transcriptional activity increased markedly in activated embryos, and immunocytochemistry with antibodies recognizing specific histone modifications revealed differential modification of several histones in the trophectoderm (TE) and inner cell mass (ICM) of dormant and activated embryos. High levels of histone H3 lysine 9 (H3K9) dimethylation, which suppresses gene expression, were observed in the ICM, but not in the TE, of dormant embryos, and the levels decreased when the embryos were activated, consistent with changes in transcriptional activity. Substitution of histone H3.3, a variant of H3, for dominant H3.1 increased in activated embryos, suggesting that histone substitution is involved in inducing gene expression associated with activation. In the nucleus, H3.3 was mainly localized in the nucleoli of activated embryos but not in those of dormant ones. In contrast, there were no obvious differences in the trimethylation of histone H3K9 or the acetylation of histones H3K9, H3K18 and H4K12 between dormant and activated embryos. These results suggest that a decrease in H3K9 dimethylation contributes to the acquisition of implantation competence by releasing genes from suppression. In addition, histone H3.3 substitution seems to be involved in global gene activation and facilitates the prompt recovery of dormant blastocysts to the active state by inducing rRNA synthesis, resulting in an increase in translational activity.
  • Y Satoh; T Mano; H Tsuruga; R Masuda; T Matsuhashi; M Onuma; M Suzuki; N Ohtaishi
    JAPANESE JOURNAL OF VETERINARY RESEARCH 50 4 195 - 199 2003年02月 [査読有り]
     
    To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes : Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB 3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido.
  • T Matsuhashi; R Masuda; T Mano; K Murata; A Aiurzaniin
    ZOOLOGICAL SCIENCE 18 8 1137 - 1143 2001年11月 [査読有り]
     
    Previous phylogenetic studies using mitochondrial DNA sequences of the brown bear Ursus arctos have separately defined two major lineages in Europe, three in Alaska, and three in Hokkaido Island of Japan. To reconstruct phylogenetic relationships among worldwide populations of the species, nucleotide sequences of the mitochondrial DNA control region and cytochrome b were determined for some additional subpopulations of Asia (Gobi and Tibetan), and then all the data including previously reported sequences were compared. The resultant phylogenetic trees showed that the worldwide populations could be grouped into at least five lineages. One of the five lineages had a wide distributional range covering Eurasia, Alaska, and central Hokkaido. Moreover, it is likely that populations from eastern Hokkaido and eastern Alaska are the direct derivatives of a single lineage. These results suggest that brown bears may have widely colonized Eurasia and North America from their original areas somewhere in Eurasia more than once.
  • T Matsuhashi; R Masuda; T Mano; MC Yoshida
    MOLECULAR BIOLOGY AND EVOLUTION 16 5 676 - 684 1999年05月 [査読有り]
     
    We investigated nucleotide sequences of the mitochondrial DNA control region to describe natural genetic variations and to assess the relationships between subpopulations of the brown bear Ursus arctos on Hokkaido Island, Japan. Using the polymerase chain reaction product-direct sequencing technique, partial sequences (about 930 bases) of the control region were determined for 56 brown bears sampled throughout Hokkaido Island. A sequence alignment revealed that the brown bear control region included a variable sequence on the 5' side and a repetitive region on the 3' side. Phylogenetic trees reconstructed from the 5' variable region (696-702 bases) exhibited 17 haplotypes, which were clustered into three groups (Clusters A, B, and C). The distribution of each group did not overlap with those of the others, and the three different areas were located in separate mountainous forests of Hokkaido Island. Furthermore, most of the phylogenetically close haplotypes within each group were distributed geographically close to each other. In addition, the 3' repetitive region (arrays of 10 bases) exhibited a much faster mutation rate than the 5' variable region, resulting in heteroplasmy. Such mitochondrial DNA divergence in each group could have occurred after the brown bears migrated from the continent to Hokkaido and became fixed in the different areas.

講演・口頭発表等

  • 笠原喜斗; 笹部冴子; 池上春香; 大林賢伍; 向島幸司; 越智浩介; 宮本圭; 松橋珠子; 松本和也; 松本和也; 松本和也
    日本畜産学会大会講演要旨 2019年03月
  • 松橋珠子; 池上春香; 越智浩介; 本廣多胤; 東口奈那美; 大林賢伍; 向島幸司; 高取等; 邨上正幸; 渡邉智; 笠原喜斗; 永井宏平; 宮本圭; 吉廣卓哉; 松本和也; 松本和也; 松本和也
    日本畜産学会大会講演要旨 2019年03月
  • 黒毛和種の枝肉形質と関連する血中バイオマーカー候補マイクロRNAの探索  [通常講演]
    松橋 珠子; 柴田成基; 笹部冴子; 池上 春香; 越智浩介; 大林賢伍; 永井 宏平; 宮本 圭; 松本 和也
    第68回関西畜産学会 2018年09月 口頭発表(一般)
  • 越智浩介; 池上春香; 松橋珠子; 大林賢伍; 永井宏平; 坂口慎一; 松本和也
    日本畜産学会大会講演要旨 2018年03月
  • 松橋珠子; 池上春香; 越智浩介; 大林賢伍; 佐野文美; 森隆史; 本廣多胤; 奥野智美; 神谷拓磨; 永井宏平; 宮本圭; 吉廣卓哉; 坂口慎一; 松本和也
    日本畜産学会大会講演要旨 2018年03月
  • 黒毛和種におけるAnnexin A5遺伝子多型と繁殖成績との関連性の検討  [通常講演]
    松橋珠子; 池上春香; 米澤康滋; 大林賢伍; 越智浩介; 奥野智美; 神谷拓磨; 宮本 圭; 坂口慎一; 松本和也
    第67回関西畜産学会大阪大会 2017年09月 口頭発表(一般)
  • 松橋珠子; 小林栄治; 池上春香; 森本学; 笹子奈々恵; 松本和也
    日本畜産学会大会講演要旨 2017年03月
  • 黒毛和種去勢牛の枝肉成績と関連する血清中バイオマーカー候補マイクロRNAの探索  [通常講演]
    松橋 珠子; 池上 春香; 森本 学; 永井 宏平; 山口 壮輝; 塚口 智将; 樋口 智香; 守田 昂太郎; 宮本 圭; 坂口 慎一; 松本 和也
    平成28年度(第66回)関西畜産学会香川大会 2016年10月 口頭発表(一般)
  • 池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也
    日本畜産学会大会講演要旨 2016年03月
  • 池上春香; 松橋珠子; 永井宏平; 塚口智将; 内堀翔; 樋口智香; 守田昂太郎; 小林直彦; 松本和也; 松本和也
    日本畜産学会大会講演要旨 2015年09月
  • 丸山新; 松橋珠子; 向島幸司
    日本畜産学会大会講演要旨 2015年03月
  • 高須賀晶子; 齊藤陽介; 佐藤邦雄; 中村亮一; 小林宙; 松橋珠子; 鈴木晶夫; 瀬戸口浩二; 佐々木慎二; 早川磨紀男; 辻岳人; 小島孝敏
    日本畜産学会大会講演要旨 2015年03月
  • 松橋珠子; 渡邊敏夫; 丸山新; 高原伸一; 山田英信; 杉本喜憲; 米山英雄
    日本畜産学会大会講演要旨 2015年03月
  • 黒毛和種牛の脂肪交雑度を予測する血清マイクロRNAの探索  [通常講演]
    池上 春香; 丹羽 尚人; 永井 宏平; 塚口 智将; 樋口 智香; 守田 昂太郎; 松橋 珠子; 小林 直彦; 松本 和也
    日本畜産学会大会講演要旨集 2015年03月
  • IARS異常症とは異なる虚弱子牛症候群の解析  [通常講演]
    岡崎 惇朗; 平野 貴; 松橋 珠子; 佐々木 慎二; 鈴木 穣; 原 ひろみ; 杉本 喜憲; 半澤 恵
    日本畜産学会大会講演要旨集 2015年03月
  • 遺伝病 ~もう一度見直しましょう!~  [招待講演]
    松橋 珠子
    岐阜県家畜人工授精師協会岐阜支部研修会 2014年03月 公開講演,セミナー,チュートリアル,講習,講義等
  • 黒毛和種牛の脂肪交雑度を予測するタンパク質バイオマーカーの探索  [通常講演]
    池上 春香; 松橋 珠子; 赤尾 大樹; 武本 淳史; 永井 宏平; 内堀 翔; 小林 直彦; 松本 和也
    日本畜産学会大会講演要旨集 2014年03月
  • 永井 宏平; 池上 春香; 松橋 珠子; 武本 淳史; 南方 佑介; 樋口 智香; 守田 昂太郎; 小林 直彦; 松本 和也
    日本プロテオーム学会大会要旨集 2014年
  • 黒毛和種のイソロイシルtRNA合成酵素(IARS)異常症劣性ホモ子牛と正常発育子牛の各月齢における血清アミノ酸濃度の比較  [通常講演]
    鹿野 達也; 山崎 ふうこ; 富岡 美千子; 菊地 薫; 阿部 ふみ; 田高 恵; 松橋 珠子; 平野 貴; 杉本 喜憲; 渡辺 大作
    日本獣医学会学術集会講演要旨集 2013年08月
  • 黒毛和種繁殖雌牛におけるIARS遺伝子型と子牛生産性との関連  [通常講演]
    松橋 珠子; 武田 賢治; 平野 貴; 西村 翔太; 佐々木 慎二; 杉本 喜憲; 北 和夫
    日本畜産学会大会講演要旨集 2013年03月
  • 丸山 新; 松橋 珠子; 小林 直彦; 中橋 良信; 口田 圭吾
    肉用牛研究会報 2013年01月
  • 子牛虚弱症候群(WCS) について  [招待講演]
    松橋 珠子
    飛騨家畜人工授精師協会技術研修会 2012年09月 公開講演,セミナー,チュートリアル,講習,講義等
  • 飛騨牛の生産性に関与する遺伝子の解明  [招待講演]
    松橋 珠子
    岐阜県家畜人工授精師協会岐阜支部技術研修会 2012年03月 公開講演,セミナー,チュートリアル,講習,講義等
  • 池上 春香; 松橋 珠子; 武本 淳史; 永井 宏平; 樋口 智香; 守田 昂太郎; 小林 直彦; 松本 和也
    日本プロテオーム学会大会要旨集 2012年
  • 飛騨牛の生産性に関与する遺伝子の解明  [招待講演]
    松橋 珠子
    岐阜県家畜人工授精師協会技術研修会 2011年11月 公開講演,セミナー,チュートリアル,講習,講義等
  • 飛騨牛の生産性に関与する遺伝子の解明  [招待講演]
    松橋 珠子
    西濃人工授精師会研修会 2011年09月 公開講演,セミナー,チュートリアル,講習,講義等
  • 飛騨牛の生産性に関与する遺伝子の解明  [招待講演]
    松橋 珠子
    岐阜県獣医師会県職部会 2011年06月 公開講演,セミナー,チュートリアル,講習,講義等
  • 池上 春香; 武本 淳史; 松橋 珠子; 森本 康一; 小林 直彦; 北 和夫; 松本 和也
    日本プロテオーム学会大会要旨集 2011年
  • エゾヒグマの遺伝学的特性と生物地理  [招待講演]
    松橋 珠子
    平成22年度大学改革推進等補助金(大学改革推進事業)に もとづく自治体関係者向け講演会 2010年12月 公開講演,セミナー,チュートリアル,講習,講義等
  • 裏山系ハンターの日常:生活の中のイノシシ猟  [招待講演]
    松橋 珠子
    2010年度日本哺乳類学会自由集会 ニッポンのハンターを絶滅から救え! 2010年09月 シンポジウム・ワークショップパネル(公募)
  • 史 琳晶; 井上 悦子; 吉廣 卓哉; 永井 宏平; 池上 春香; 松橋 珠子; 小林 直彦; 森本 康一; 松本 和也; 中川 優
    全国大会講演論文集 2008年03月

MISC

産業財産権

受賞

  • 2018年09月 関西畜産学会 平成30年度関西畜産学会賞
     黒毛和種集団の遺伝的能力を改善するマーカー同定に関する研究 
    受賞者: 松橋 珠子

共同研究・競争的資金等の研究課題

  • 日本学術振興会:科学研究費助成事業
    研究期間 : 2021年04月 -2025年03月 
    代表者 : 猪島 康雄; 岡田 彩加; 高島 茂雄; 松橋 珠子
  • 日本学術振興会:科学研究費助成事業
    研究期間 : 2020年04月 -2024年03月 
    代表者 : 松本 和也; 根本 充貴; 松橋 珠子
     
    本研究では、我々は肉用牛の肥育期間中に経時的に採取した血清を対象とした質量分析機を用いた定量プロテオミクスSWATH-MS(Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry)解析情報に基づき世界に先駆けて実施している「血清中バイオマーカータンパク質の動態と種類に関する情報から肉用牛の肥育状態を肥育期間中に生体評価するシステム」の開発研究を積み上げている。これは、畜産学と情報科学との異分野融合研究によって、肉用牛の産肉形質を生体評価するシステムの開発と、その結果得られる血清バイオマーカータンパク質の情報に基づく産肉制御の分子機構の統合的理解を目的とするものである。さらに、研究の成果は、これまでその詳細が不明であった肥育期間中の肉用牛における産肉形質の発育に関与する分子機構の解明に資する新しい知見の獲得が期待される。今後、本研究成果の生体評価システムが肉用牛の生産現場に実装された場合、農家が持つ経験値に依存した農業経営から科学的根拠に基づく農業経営への転換の基盤構築に貢献できる。 2年目の成果として、初年度に基盤構築した、日本の地域性の異なる3地域と全国レベルの肉用牛を扱っている研究機関の協力を得て、供試された出荷時の肉用牛(黒毛和種去勢)の枝肉成績の情報と肥育期間中のウシ血清サンプルに基づいた肉用牛の産肉形質の発育状態や肥育状態を生体評価するシステムを用いて、血清バイオマーカータンパク質の情報を基盤とした産肉制御の分子機構の統合的理解を目指して分子間パスウェイネットワーク解析ソフト(IPA)を導入し、産肉形質の形成過程における血清バイオマーカータンパク質の動態から、脂肪交雑の発育シミュレーションモデルを作成した。
  • 日本学術振興会:科学研究費助成事業
    研究期間 : 2016年04月 -2018年03月 
    代表者 : 松本 和也; 永井 宏平; 松橋 珠子
     
    本研究では、肥育期間中にと畜時の枝肉成績を予測診断する指標として、血中に含まれるエクソソームに内包された分泌型miRNAからバイオマーカーの探索を実施した。miRNAマイクロアレイ解析の結果、18種類の分泌型miRNAをバイオマーカー候補としてリストアップし、さらに血中miRNAの定量分析と枝肉形質との関連の解析により、バイオマーカー候補のうち2種類のmiRNAについて再現性が確認された。国内3地域の肥育中血清を比較した結果、miRNA量に地域差が検出された。

担当経験のある科目

  • 動物生産学近畿大学
  • 公衆衛生学近畿大学

社会貢献活動

  • 北の大地にヒグマの姿を求めて
    期間 : 2007年12月18日 - 2008年07月14日
    役割 : 講師
    種別 : 出前授業
    主催者・発行元 : 岐阜県立飛騨高山高等学校

その他のリンク

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