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田辺 寛之タナベ ヒロユキ

プロフィール

所属部署名農学部 バイオサイエンス学科 / 農学研究科
職名教授
学位農学博士
専門基礎老化学
ジャンル科学・技術/遺伝子・DNA技術
コメンテータガイドhttp://www.kindai.ac.jp/meikan/485-tanabe-hiroyuki.html
ホームページURLhttp://kaken.nii.ac.jp/d/r/40257986.ja.html
メールアドレス
Last Updated :2017/09/14

コミュニケーション情報 byコメンテータガイド

コメント

    ヒトの寿命を決める仕組みの解明に取り組んでいます。これまでの研究結果に基づいて作製した遺伝子改変マウスをモデル生物として本研究を進めています。

学歴・経歴

経歴

  •   1995年,  - 1997年, 近畿大学(講師)

研究活動情報

研究キーワード

  • microRNA, reproduction, aging, lifespan

論文

  • Lifespan and reproduction in brain-specific miR-29-knockdown mouse, 田辺寛之, Biochemical and Biophysical Research Communications, 471, 4, 454, 458,   2016年, 査読有り
    概要:The microRNA miR-29 is widely distributed and highly expressed in adult mouse brain during the mouse's lifetime. We recently created conditional mutant mice whose miR-29 was brain-specifically knocked down through overexpression of an antisense RNA transgene against miR-29. To explore a role for brain miR-29 in maximizing organismal fitness, we assessed somatic growth, reproduction, and lifespan in the miR-29-knockdown (KD) mice and their wild-type (WT) littermates. The KD mice were developmentally indistinguishable from WT mice with respect to gross morphology and physical activity. Fertility testing revealed that KD males were subfertile, whereas KD females were hyperfertile, only in terms of reproductive success, when compared to their gender-matched WT correspondents. Another phenotypic difference between KD and WT animals appeared in their lifespan data; KD males displayed an overall increasing tendency in post-reproductive survival relative to WT males. In contrast, KD females were prone to shorter lifespans than WT females. These results clarify that brain-targeted miR-29 knockdown affects both lifespan and reproduction in a gender-dependent manner, and moreover that the reciprocal responsiveness to the miR-29 knockdown between these two phenotypes in both genders closely follow life-course models based on the classical trade-off prediction wherein elaborate early-life energetic investment in reproduction entails accelerated late-life declines in survival, and vice versa. Thus, this study identified miR-29 as the first mammalian miRNA that is directly implicated in the lifetime trade-off between the two major fitness components, lifespan and reproduction.
  • Small RNAs in mouse brain specifically expressed at the end-life stage (NAID: 120003088627), 田辺 寛之, Memoirs of the Faculty of Agriculture of Kinki University, 44, 115, 122,   2011年04月
    概要:The organismal life span is supposed to be mechanistically linked to the system of sexual reproduction specified by a yet uncharacterized species-specific life program. On the basis of previous studies, we predicted that small non-coding RNA serves as the primary signal that directs the termination of the maximum life span inherent in each species. Here, we prepared small-sized transcripts from the whole brain of newborn mice and extremely aged ones, and compared the transcriptome of the two age groups by deep sequencing. Annotation of their transcriptional differences identified a few kinds of microRNA markedly up-regulated in the older mouse brain. In particular, the miR-29 family was noteworthy for its high expression level. A functional attribution to this microRNA in the molecular mechanism for the determination of the murine life span was proposed.
  • Global profiling of gene expression in mouse astrocyte in response to the potential longevity determinant miR-29 (NAID: 120004400637), 田辺 寛之, Memoirs of the Faculty of Agriculture of Kinki University, 45, 1, 16,   2012年04月
    概要:Our previous study identified the miR-29 family, three paralogous species of miR-29a/b/c, as the most predominantly expressed small RNA in aged mouse brain compared to the neonate one. To explore the functional relevance of miR-29 expression in early life to the neural physiology of the mouse brain from the mechanistic perspective of mammalian species-specific lifespan, we here undertook a gain-of-function approach through exogenous expression of miR-29 in astrocyte from mouse fetus and surveyed the resulting alteration in both the transcriptional and translational levels. Our present results strongly suggest that miR-29 in the developing mouse brain serves as the central coordinator to shift the global gene expression toward adult phenotypes, through which ensure the programmed transition in the life course to the post-developmental/reproductive stage which has inherently been set to delimit the mouse life potential.
  • Generation and some characterization of BAC transgenic mouse that neurally expresses antisense RNA against the potential longevity determinant miR-29 (NAID: 120005298690), 田辺 寛之, Memoirs of the Faculty of Agriculture of Kinki University, 46, 1, 14,   2013年04月
    概要:miR-29, a gene family of microRNA, is highly expressed across the entire adulthood until life termination in the mouse central nervous system. Given a leading part that microRNAs play in a broad spectrum of biological events, it is convincing to hypothesize that miR-29 present in the central nervous system is causally involved in the setting of the maximum duration of mammalian life. Here we set out a BAC transgenic mouse that displays brain-specific conditional knockdown of miR-29 by overexpressing antagonistic transcripts against this microRNA. Analysis of one representative founder line revealed a substantial decrease in the endogenous miR-29 level only in the brain compared with non-transgenic littermates.