KINDAI UNIVERSITY


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谷口 亮人タニグチ アキト

プロフィール

所属部署名農学部 水産学科 / 農学研究科
職名講師
学位博士(農学)
専門微生物海洋学
ジャンル環境/気象・海洋
コメンテータガイドhttp://www.kindai.ac.jp/meikan/941-taniguchi-akito.html
ホームページURLhttp://kaken.nii.ac.jp/d/r/10548837.ja.html
メールアドレスhtrakito[at]nara.kindai.ac.jp
Last Updated :2017/11/22

コミュニケーション情報 byコメンテータガイド

コメント

    海洋生態系における微生物群の組成や活性について研究しています。特に、活発に増殖している細菌に注目しています。延べ航海日数180日以上、延べ調査日数350日以上の経験があります。

学歴・経歴

学歴

  • 2006年06月 - 2008年03月, 東京大学, 海洋研究所, 特別研究生
  • 2005年04月 - 2008年03月, 広島大学大学院, 生物圏科学研究科, 博士課程後期
  • 2003年04月 - 2005年03月, 広島大学大学院, 生物圏科学研究科, 博士課程前期
  • 1999年04月 - 2003年03月, 広島大学, 生物生産学部, 生物生産学科

経歴

  •   2016年04月,  - 現在, 近畿大学(講師)
  •   2013年04月,  - 2016年03月, 近畿大学(助教)
  •   2009年01月,  - 2013年03月, 近畿大学(GCOE博士研究員)
  •   2008年04月,  - 2008年12月, 東京大学(特別研究員)

研究活動情報

研究分野

  • 環境学, 環境動態解析
  • 水圏応用科学, 水圏生産科学

研究キーワード

  • 生態学, 海洋学, 多様性, 微生物

論文

  • Active populations of rare microbes in oceanic environments as revealed by bromodeoxyuridine incorporation and 454 tag sequencing, Hamasaki Koji, Taniguchi Akito, Tada Yuya, Kaneko Ryo, Miki Takeshi, GENE, 576, 2, 650, 656,   2016年02月01日, 査読有り
  • Community structures of actively growing bacteria stimulated by coral mucus, Akito Taniguchi, Takashi Yoshida, Kodai Hibino, Mitsuru Eguchi, Journal of Experimental Marine Biology and Ecology, 469, 105, 112,   2015年08月01日, 査読有り
    概要:© 2015 Elsevier B.V. Coral mucus influences the composition and abundance of bacteria in the surrounding seawater. In this study, the phylogenetic affiliations of actively growing bacteria (AGB) in seawater supplemented with the mucus of Acropora sp. during a 24-h incubation period were determined. For this purpose, bromodeoxyuridine magnetic-beads immunocapture and PCR-DGGE (BUMP-DGGE) analysis was used. The coral mucus contained higher concentrations of particulate organic carbon and nitrogen and exhibited higher bacterial abundance than seawater did, and the organic matter and bacteria varied dramatically during the incubation. BUMP-DGGE analysis showed that the AGB stimulated by the coral mucus also varied during the incubation. The bulk of the active growers originated from seawater and not the mucus. However, not all of the bacterial phylotypes in seawater could use the coral mucus as a suitable growth substrate. Cluster analysis revealed that the AGB community structures identified across the incubation period were considerably different in seawater supplemented with coral mucus as compared with those in coral mucus. In total, 12 phylotypes of AGB were identified, and these belonged to Alphaproteobacteria (Rhodobacterales 5 phylotypes), Gammaproteobacteria (Oceanospirillales 3 phylotypes, Vibrionales 1 phylotype, Alteromonadales 1 phylotype), and Bacteroidetes (Flavobacteriales 2 phylotypes). In the coral mucus, phylotypes belonging to Oceanospirillales appeared only at the beginning of the incubation, whereas those belonging to Rhodobacterales appeared only in the late phase of the incubation. Thus, coral mucus can alter the AGB community structure and create an organic matter flux that is specific to a reef environment.
  • Bacterial production is enhanced by coral mucus in reef systems, Akito Taniguchi, Takashi Yoshida, Mitsuru Eguchi, Journal of Experimental Marine Biology and Ecology, 461, 331, 336,   2014年12月01日, 査読有り
    概要:© 2014 Elsevier B.V. Coral mucus influences the abundance and composition of microbes in the surrounding seawater. Here, the hypothesis that the mucus of the coral genera Acropora, Favia, Favites, and Goniastrea would influence bacterial growth (production) in seawater (measured using 3H-thymidine and 3H-leucine incorporation methods) was tested. The bacterial production in mucus-supplemented seawater was significantly greater than that in unsupplemented seawater: production in the mucus-supplemented seawater ranged from 108.2 to 4111μgC·L-1·d-1 when measured by thymidine incorporation and from 72.9 to 115.9μgC·L-1·d-1 when measured by leucine incorporation. This study showed that the enhanced production may be due to bacteria originating from seawater and not from coral mucus. Nonetheless, the effect of mucus on bacterial production was different among the coral genera: the mucus of Favia did not always enhance bacterial production. Additionally, even a very small amount of coral mucus, only ~300μL of mucus per liter of seawater, was sufficient to enhance bacterial production by approximately 2-fold compared to unsupplemented seawater. These results suggest that coral mucus has a significant impact on the marine biogeochemical cycle around coral reefs.
  • Microbial communities in various waters used for fish larval rearing, Akito Taniguchi, Ryuichiro Aoki, Mitsuru Eguchi, Aquaculture Research, 47, 2, 370, 378,   2014年06月16日, 査読有り
    概要:A major concern in larvae production is a mass mortality caused by fish diseases. In larvae production, pumped-up natural seawater filtered through a sand filter system is used for fish rearing, and microalgae and rotifer cultures. Here, we investigated the community structures of eukaryotic microbes, as well as total bacteria and vibrios, in various processed 'waters' used in a larvae production site. We observed that ultraviolet irradiation of seawater was effective to reduce not only total bacteria and vibrios but also eukaryotic microbes. Moreover, the community structures of total bacteria and vibrios in rearing waters for fish larvae were different from those in rotifer cultures fed with Chlorella, but rather similar to those in natural seawater and microalgae cultures. These results suggest that the bacterial community in rearing waters may originate mainly from natural seawater and then be selected by microalgae in rearing water. Overall, this study provides useful information for avoiding the risk of fish disease outbreaks in a larvae production site. © 2014 John Wiley & Sons Ltd.
  • Growth and distribution patterns of Roseobacter/Rhodobacter, SAR11, and Bacteroidetes lineages in the Southern Ocean, Yuya Tada, Ryosuke Makabe, Nobue Kasamatsu-Takazawa, Akito Taniguchi, Koji Hamasaki, Polar Biology, 36, 5, 691, 704,   2013年02月11日, 査読有り
    概要:Roseobacter/Rhodobacter and SAR11, affiliated with Alphaproteobacteria, and the phylum Bacteroidetes constitute a large proportion of marine planktonic bacteria, but information about their growth and distribution patterns in the Southern Ocean is scarce. The aim of the present study is to determine patterns in the biomass and productivity of Roseobacter/Rhodobacter, SAR11, and Bacteroidetes groups along the steep temperature, salinity, and organic matter gradients in the Southern Ocean by using catalyzed reporter deposition-fluorescence in situ hybridization and bromodeoxyuridine (BrdU) immunocytochemistry FISH. We found that Roseobacter/Rhodobacter, SAR11, and Bacteroidetes are prominent contributors to total bacterial biomass and production. SAR11 bacteria were the predominant lineage, but their biomass was low in the coldest regions. In contrast, the biomasses of Roseobacter/Rhodobacter and Bacteroidetes lineages were positively correlated with organic matter concentrations. The Roseobacter/Rhodobacter had the highest proportion of BrdU-positive (i. e., actively growing) cells among the three phylotypes at all stations, despite their low abundance. The relative contribution of Bacteroidetes to the total bacterial productivity (number of active cells) was negatively correlated with temperature. These results suggest that the growth and distribution patterns of Roseobacter/Rhodobacter, SAR11, and Bacteroidetes were determined by different environmental gradients (e. g., organic matter concentrations or temperature) in the Southern Ocean. © 2013 Springer-Verlag Berlin Heidelberg.
  • Growth and succession patterns of major phylogenetic groups of marine bacteria during a mesocosm diatom bloom, Yuya Tada, Akito Taniguchi, Yuki Sato-Takabe, Koji Hamasaki, Journal of Oceanography, 68, 4, 509, 519,   2012年08月01日, 査読有り
    概要:Our objective was to track microbial processes associated with serial degradation of organic matter derived from algal blooms. To do this, we analyzed population fluctuations and growth responses of major phylogenetic groups of free-living marine bacteria. We used bromodeoxyuridine immunocytochemistry-fluorescence in situ hybridization methodology to examine marine bacterial community development during and after a diatom bloom in a mesocosm. We revealed that the Roseobacter/Rhodobacter, SAR11, Alteromonas, and Bacteroidetes groups were clearly major phylotypes responsible for most free-living bacterial biomass and production throughout the experiment. The clearest bacterial response was a proliferation of the Alteromonas group (cells with large volumes) during development of the bloom (up to 30 % of actively growing cells). Populations of these bacteria declined sharply thereafter, likely due to grazing. Alteromonas group responses suggest that these bacteria strongly influenced the flux of organic matter at an early bloom stage. The growth potential of Bacteroidetes was relatively large as the bloom peaked; this early development probably contributed to the initial stage of bloom decomposition. In contrast, the contribution of Roseobacter/Rhodobacter to total bacterial production increased at a late stage of decomposing of the bloom. The contributions of Betaproteobacteria, SAR11, and SAR86 groups to total bacterial abundance and production were relatively minor throughout the experiment. These results imply that the ability to utilize organic matter derived from diatoms varies among bacterial phylotypes, and, frequently, less abundant but ecological specialist taxa such as Alteromonas may play major roles in the flux of organic matter during diatom blooms. © 2012 The Oceanographic Society of Japan and Springer.
  • Seasonal variations in the community structure of actively growing bacteria in Neritic waters of Hiroshima bay, Western Japan, Akito Taniguchi, Akito Taniguchi, Yuya Tada, Koji Hamasaki, Microbes and Environments, 26, 4, 339, 346,   2011年12月13日, 査読有り
    概要:Using bromodeoxyuridine (BrdU) magnetic beads immunocapture and a PCR-denaturing gradient gel electrophoresis (DGGE) technique (BUMP-DGGE), we determined seasonal variations in the community structures of actively growing bacteria in the neritic waters of Hiroshima Bay, western Japan. The community structures of actively growing bacteria were separated into two clusters, corresponding to the timing of phytoplankton blooms in the autumn-winter and spring-summer seasons. The trigger for changes in bacterial community structure was related to organic matter supply from phytoplankton blooms. We identified 23 phylotypes of actively growing bacteria, belonging to Alphaproteobacteria (Roseobacter group, 9 phylotypes), Gammaproteobacteria (2 phylotypes), Bacteroidetes (8 phylotypes), and Actinobacteria (4 phylotypes). The Roseobacter group and Bacteroidetes were dominant in actively growing bacterial communities every month, and together accounted for more than 70% of the total DGGE bands. We revealed that community structures of actively growing bacteria shifted markedly in the wake of phytoplankton blooms in the neritic waters of Hiroshima Bay.
  • Quantitative PCR assay for the detection of the parasitic ciliate Cryptocaryon irritans, Akito Taniguchi, Hiroyuki Onishi, Mitsuru Eguchi, Fisheries Science, 77, 4, 607, 613,   2011年07月01日, 査読有り
    概要:We developed a quantitative PCR assay for detecting the parasitic ciliate Cryptocaryon irritans, which causes "white spot disease" in marine fishes, from the natural environment. A specific primer set for C. irritans was designed and its high specificity was confirmed in silico: almost all of the sequences deposited in the Gen-Bank nucleotide database were covered, 22/23 for the forward primer and 7/7 for the reverse primer. We estimated that there were 3,415.9 rRNA gene copies per genome of C. irritans. In artificial mixture experiments to validate whether the qPCR assay is applicable to natural samples, the estimated copy numbers showed significantly positive correlations with the number of theronts added (p < 0.001). When we applied this qPCR assay to natural samples collected bimonthly from surface and bottom seawaters at an aquaculture site (water depth, 10 m) from May 2009 to March 2010, we only detected C. irritans (112.0 ± 6.3 cells/l) in the surface seawater sample in November. This qPCR assay is a useful tool for detecting C. irritans rapidly and quantitatively in natural environments; it could also help advance our understanding of the ecology of C. irritans, as well as facilitate the diagnosis of the disease. © 2011 The Japanese Society of Fisheries Science.
  • Differing growth responses of major phylogenetic groups of marine bacteria to natural phytoplankton blooms in the Western North Pacific Ocean, Yuya Tada, Akito Taniguchi, Ippei Nagao, Takeshi Miki, Mitsuo Uematsu, Atsushi Tsuda, Koji Hamasaki, Applied and Environmental Microbiology, 77, 12, 4055, 4065,   2011年06月01日, 査読有り
    概要:Growth and productivity of phytoplankton substantially change organic matter characteristics, which affect bacterial abundance, productivity, and community structure in aquatic ecosystems. We analyzed bacterial community structures and measured activities inside and outside phytoplankton blooms in the western North Pacific Ocean by using bromodeoxyuridine immunocytochemistry and fluorescence in situ hybridization (BICFISH). Roseobacter/Rhodobacter, SAR11, Betaproteobacteria, Alteromonas, SAR86, and Bacteroidetes responded differently to changes in organic matter supply. Roseobacter/Rhodobacter bacteria remained widespread, active, and proliferating despite large fluctuations in organic matter and chlorophyll a (Chl-a) concentrations. The relative contribution of Bacteroidetes to total bacterial production was consistently high. Furthermore, we documented the unexpectedly large contribution of Alteromonas to total bacterial production in the bloom. Bacterial abundance, productivity, and growth potential (the proportion of growing cells in a population) were significantly correlated with Chl-a and particulate organic carbon concentrations. Canonical correspondence analysis showed that organic matter supply was critical for determining bacterial community structures. The growth potential of each bacterial group as a function of Chl-a concentration showed a bell-shaped distribution, indicating an optimal organic matter concentration to promote growth. The growth of Alteromonas and Betaproteobacteria was especially strongly correlated with organic matter supply. These data elucidate the distinctive ecological role of major bacterial taxa in organic matter cycling during open ocean phytoplankton blooms. © 2011, American Society for Microbiology.
  • Phylotype-specific growth rates of marine bacteria measured by bromodeoxyuridine immunocytochemistry and fluorescence in situ hybridization, Yuya Tada, Akito Taniguchi, Koji Hamasaki, Aquatic Microbial Ecology, 59, 3, 229, 238,   2010年07月09日, 査読有り
    概要:To explore the possibility of calculating phylotype-specific growth rates of marine bacteria, we optimized a protocol that combines bromodeoxyuridine (BrdU) immunocytochemistry with fluorescence in situ hybridization (FISH). The protocol worked well with 8 isolates tested, showing a significant positive correlation between average cellular fluorescence intensity and cell-specific BrdU contents. The BrdU immunocytochemistry-FISH (BIC-FISH) was then applied to neritic seawater in Hiroshima Bay, western Japan. Rhodobacter/Roseobacter and Bacteroidetes groups were dominant in BrdU-positive cells (22 ± 8 and 26 ± 8%), suggesting a significant contribution to bacterial community productivity in the water. Analysis of single-cell immunofluorescence showed higher BrdU incorporation rates in the Gammaproteobacteria, SAR86, and Vibrio groups, suggesting that these less abundant groups were growing more rapidly than other phylotypes. Our study suggests that BIC-FISH has the potential to estimate phylotype-specific variability of bacterial productivity in aquatic systems. © Inter-Research 2010.
  • Phylotype-specific productivity of marine bacterial populations in eutrophic seawater, as revealed by bromodeoxyuridine immunocytochemistry combined with fluorescence in situ hybridization, Yuya Tada, Akito Taniguchi, Koji Hamasaki, Microbes and Environments, 24, 4, 315, 321,   2009年09月03日, 査読有り
    概要:Among the fundamental questions in marine microbial ecology are which taxa or phylogenetic groups account for total bacterial productivity and what is the relative contribution of each. We combined bromodeoxyuridine (BrdU) immunocytochemistry and fluorescence in situ hybridization (BIC-FISH) to examine phylotype-specific contributions to total bacterial productivity in eutrophic seawater. We also examined year-round changes in phylotype-specific contributions and explored the factors controlling these changes. Monitoring by BIC-FISH throughout the year revealed the importance of the Roseobacter/Rhodobacter group as a constantly proliferating basic population (27% of all BrdUpositive cells), although their contribution was not significantly correlated with water temperature or with chlorophyll a or organic matter concentration. The Bacteroidetes were another important group, as they greatly increased in abundance after the end of phytoplankton blooms. Two other phylotypes tested, the SAR86 and Vibrio groups, changed their contributions to bacterial productivity with changes in water temperature. To our knowledge, this study was the first to estimate the yearly contribution of major subgroups of marine bacteria to total bacterial productivity in a seawater environment.
  • Analysis of nanoplankton community structure using flow sorting and molecular techniques, Noriaki Yoshida, Masahiko Nishimura, Katsuyuki Inoue, Susumu Yoshizawa, Eriko Kamiya, Akito Taniguchi, Koji Hamasaki, Kazuhiro Kogure, Microbes and Environments, 24, 4, 297, 304,   2009年09月03日, 査読有り
    概要:We developed a method for the separate and simultaneous analysis of the community structure of heterotrophic nanopkankton (HNP) and autotrophic nanoplankton (ANP). This method consists of three steps. First, nanoplankton cells were concentrated using a cross-flow filtration system because cell densities in natural seawater are usually too low for genetic studies. Second, HNP and ANP were separated by flow cytometric sorting ("flow sorting") on the basis of the presence or absence of chlorophyll. Finally, the community structure was analyzed using denaturing gradient gel electrophoresis targeting 18S rRNA gene. The newly developed method was applied to the coastal surface water of Aburatsubo Inlet, Japan, in July 2008. The separation of nanoplankton into HNP and ANP was validated by phylogenetic analysis, and the trophic mode of uncultured nanoplankton was confirmed (e.g. Marine Alveolata group II [MALV II] and Marine Stramenopile clade-2 [MAST-2]). This new method involving cell concentration, flow sorting and phylogenetic analysis is a potentially powerful tool for evaluating the population dynamics and ecology of marine protozoa.
  • Community structures of actively growing bacteria shift along a north-south transect in the western North Pacific, Akito Taniguchi, Koji Hamasaki, Environmental Microbiology, 10, 4, 1007, 1017,   2008年04月01日, 査読有り
    概要:Bacterial community structures and their activities in the ocean are tightly coupled with organic matter fluxes and thus control ocean biogeochemical cycles. Bromodeoxyuridine (BrdU), halogenated nucleoside and thymidine analogue, has been recently used to monitor actively growing bacteria (AGB) in natural environments. We labelled DNA of proliferating cells in seawater bacterial assemblages with BrdU and determined community structures of the bacteria that were possible key species in mediating biochemical reactions in the ocean. Surface seawater samples were collected along a north-south transect in the North Pacific in October 2003 and subjected to BrdU magnetic beads immunocapture and PCR-DGGE (BUMP-DGGE) analysis. Change of BrdU-incorporated community structures reflected the change of water masses along a north-south transect from subarctic to subtropical gyres in the North Pacific. We identified 25 bands referred to AGB as BrdU-incorporated phylotypes, belonging to Alphaproteobacteria (5 bands), Betaproteobacteria (1 band), Gammaproteobacteria (4 bands), Cytophaga-Flavobacterium-Bacteroides (CFB) group bacteria (5 bands), Gram-positive bacteria (6 bands), and Cyanobacteria (4 bands). BrdU-incorporated phylotypes belonging to Vibrionales, Alteromonadales and Gram-positive bacteria appeared only at sampling stations in a subtropical gyre, while those belonging to Roseobacter-related bacteria and CFB group bacteria appeared at the stations in both subarctic and subtropical gyres. Our result revealed phylogenetic affiliation of AGB and their dynamic change along with north-south environmental gradients in open oceans. Different species of AGB utilize different amount and kinds of substrates, which can affect the change of organic matter fluxes along transect. © 2007 The Authors.
  • Actively growing bacteria in the Inland Sea of Japan, identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis, Koji Hamasaki, Koji Hamasaki, Akito Taniguchi, Yuya Tada, Richard A. Long, Richard A. Long, Farooq Azam, Applied and Environmental Microbiology, 73, 9, 2787, 2798,   2007年05月01日, 査読有り
    概要:A fundamental question in microbial oceanography concerns the relationship between prokaryote diversity and biogeochemical function in an ecosystem context We combined bromodeoxyuridine (BrdU) magnetic bead immunocaptore and PCR-denataring gradient gel electrophoresis (BUMP-DGGE) to examine pliylotype-specific growth in natural marine assemblages. We also examined a broad range of marine bacterial isolates to determine their abilities to incorporate BrdU in order to test the validity of the method for application to diverse marine assemblages. We found that 27 of 29 isolates belonging to different taxa could incorporate BrdU. BUMP-DGGE analysis revealed phylogenetic affiliations of BNA-synthesizing, presumably actively growing bacteria across a eutrophic to mesotrophic transect in the Inland Sea of Japan. We found that the BrdU-incorporating (growing) communities were substantially different from the total communities. The majority (34/56) of phylotypes incorporated BrdU and were presumably growing, and these phylotypes comprised 10 alphaproteobacteria, 1 betaproteobacterium, 11 gammaproteobacteria, 11 Cytophaga-Flavobacterium- Bacteroides group bacteria, and 1 unclassified bacterium. All BrdU-responsive alphaproteobacteria were members of the Rhodobacterales, suggesting that such bacteria were dominant in the growing alphaproteobacterial populations in our samples. The BrdU-responsive gammaproteobacteria belonged to the Oceanospirillales, the SAR86 cluster, the Pseudomonadales, the Alteromonadales, and the Vibrionales. Thus, contemporaneous cooccurrence of diverse actively growing bacterial taxa was a consistent pattern in our biogeochemically varied study area. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

講演・口頭発表等

  • 田辺湾養殖漁場の底泥に蓄積する有機物量の増減パターン, 中瀬 玄徳, 江口 充, 家戸 敬太郎, 宮下 盛, 谷口 亮人, 諏訪 剛, 平成24年度日本水産学会春季大会,   2012年03月, 平成24年度日本水産学会春季大会

MISC

  • Microbial communities in various waters used for fish larval rearing, Akito Taniguchi, Ryuichiro Aoki, Mitsuru Eguchi, Aquaculture Research, 47, 370, 378,   2016年02月01日, 10.1111/are.12495, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84953345735&origin=inward
    概要:© 2016 John Wiley & Sons Ltd. A major concern in larvae production is a mass mortality caused by fish diseases. In larvae production, pumped-up natural seawater filtered through a sand filter system is used for fish rearing, and microalgae and rotifer cultures. Here, we investigated the community structures of eukaryotic microbes, as well as total bacteria and vibrios, in various processed 'waters' used in a larvae production site. We observed that ultraviolet irradiation of seawater was effective to reduce not only total bacteria and vibrios but also eukaryotic microbes. Moreover, the community structures of total bacteria and vibrios in rearing waters for fish larvae were different from those in rotifer cultures fed with Chlorella, but rather similar to those in natural seawater and microalgae cultures. These results suggest that the bacterial community in rearing waters may originate mainly from natural seawater and then be selected by microalgae in rearing water. Overall, this study provides useful information for avoiding the risk of fish disease outbreaks in a larvae production site.
  • PA-003 クロマグロ養殖場のサンゴが細菌群の生産と組成に与える影響(水圏生態系,ポスター発表), 吉田 隆志, 青木 隆一郎, 西村 翔太, 畔柳 裕樹, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 29,   2013年11月17日, http://ci.nii.ac.jp/naid/110009973062
  • PE-017 完全養殖クロマグロ仔稚魚における消化管内細菌叢の解析(微生物群集構造,ポスター発表), 青木 隆一郎, 小原 紘平, 梶 暁登, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 29,   2013年11月17日, http://ci.nii.ac.jp/naid/110009974424
  • PAa-07 干潟における細菌による無機化活性(PAa 水圏生態系(一般),ポスター発表), 平井 真弓, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 28,   2012年09月19日, http://ci.nii.ac.jp/naid/110009972925
  • PAa-17 原核生物生産を促進するサンゴ粘液(PAa 水圏生態系(一般),ポスター発表), 吉田 隆志, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 28,   2012年09月19日, http://ci.nii.ac.jp/naid/110009974223
  • 仔稚魚飼育水での微細藻類ナンノクロロプシスによるビブリオ属細菌の制御, 谷口 亮人, Sharifah Noor Emilia, 江口 充, 水産増殖 = The aquiculture, 59, 3, 451, 458,   2011年09月20日, http://ci.nii.ac.jp/naid/10029848333
    概要:仔魚飼育水を模した、天然海水とナンノクロロプシスを添加したマイクロコスム実験を行い、ビブリオ属細菌の動態を13日間モニターした。クロロフィルa濃度は、タンク1で8.71~235μg/l、タンク2で10.9~255μg/l の範囲で変動し、両タンクとも培養6日目にピークを迎えた。培養可能なビブリオ属細菌数および定量PCR法で推定したビブリオ属細菌数は、クロロフィルa濃度の増加とともに減少した。面白いことに、クロロフィルa濃度がピークを迎えた後は、培養可能なビブリオ属細菌は検出されず、かつ定量PCR法においてもわずかな数しか検出されなかった。ビブリオ属細菌の群集構造は、実験開始時は非常に多様であったが、クロロフィルa濃度が増加するにつれ、単純化した。これらの結果は、仔魚飼育水のナンノクロロプシスは、ビブリオ属細菌の増殖を制御しており、仔魚の生残や成長の鍵を握る要因の一つとなることを示唆する。
  • メタゲノミクスで見る微生物の多様性 (特集 微生物の多様性--「遺伝子の多様性」/「細胞の多様性」から見た生物多様性), 谷口 亮人, 金子 亮, 濱崎 恒二, 遺伝, 65, 3, 61, 67,   2011年05月, http://ci.nii.ac.jp/naid/40018834803
  • P-20 リアルタイムPCR法を用いた天然養殖場水域における寄生繊毛虫Cryptocaryon irritans動態解析(A.水圏生態系,ポスター発表), 大西 宏幸, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 26,   2010年11月23日, http://ci.nii.ac.jp/naid/110009972722
  • P-151 植物プランクトン-細菌群相互作用によるVibrio属細菌の制御(K.共生/相互作用,ポスター発表), シャリファ ノル エミリア, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 26,   2010年11月23日, http://ci.nii.ac.jp/naid/110009972781
  • 1A-9 養殖場環境を健全に維持する細菌鍵種の季節的変動(E.群集構造解析,口頭発表), 谷口 亮人, 濱中 貴士, 江口 充, 日本微生物生態学会講演要旨集, 26,   2010年11月23日, http://ci.nii.ac.jp/naid/110009973863
  • P-84 養殖場環境における増殖細菌群の構造解析(ポスター発表), 谷口 亮人, 平田 惣祐, 江口 充, 日本微生物生態学会講演要旨集, 25,   2009年11月21日, http://ci.nii.ac.jp/naid/110007520463
  • P-102 植物プランクトン培養液中における細菌間相互作用(ポスター発表), シャリファ ノル エミリア, 谷口 亮人, 江口 充, 日本微生物生態学会講演要旨集, 25,   2009年11月21日, http://ci.nii.ac.jp/naid/110007520479
  • P-4 南太平洋から南大洋における稀少微生物圏の多様性(ポスター発表), 浜崎 恒二, 谷口 亮人, 日本微生物生態学会講演要旨集, 25,   2009年11月21日, http://ci.nii.ac.jp/naid/110007520393
  • 01-007 ミズクラゲ幼生の着底誘引および阻害細菌の探索(共生/相互作用,研究発表), 谷口 亮人, 神谷 英里子, 多田 雄哉, 藤井 直紀, 石井 晴人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 24,   2008年11月25日, http://ci.nii.ac.jp/naid/110007011755
  • 08-141 メソコスムにおける植物プランクトンブルーム形成及び崩壊過程に伴うナノプランクトン群衆の動態解析(水圏生態系,研究発表), 吉田 典明, 谷口 亮人, 多田 雄哉, 浜崎 恒二, 木暮 一啓, 日本微生物生態学会講演要旨集, 24,   2008年11月25日, http://ci.nii.ac.jp/naid/110007011889
  • 12-226 中栄養沿岸域における酸素非発生型好気性光合成細菌の現存量の変動(物質循環,研究発表), 佐藤 由季, 福田 秀樹, 谷口 亮人, 吉田 典明, 浜崎 恒二, 日本微生物生態学会講演要旨集, 24,   2008年11月25日, http://ci.nii.ac.jp/naid/110007011974
  • PB-70 マイクロビーズアレイシステムを用いた海洋細菌の群集構造解析(群集構造,ポスターセッションB,ポスター発表), 多田 雄哉, 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 23,   2007年09月15日, http://ci.nii.ac.jp/naid/110006480024
  • PA-27 植物プランクトンブルームの形成・崩壊過程における細菌群集の変動 : BUMP-DGGE法による解析(群集構造解析,ポスターセッションA,(1)ポスター発表会,研究発表会), 谷口 亮人, 多田 雄哉, 浜崎 恒二, 横川 太一, 茂手木 千晶, 永田 俊, 日本微生物生態学会講演要旨集, 22,   2006年10月27日, http://ci.nii.ac.jp/naid/110006212575
  • PA-28 広島湾において活発に増殖する細菌系統群の季節変動(群集構造解析,ポスターセッションA,(1)ポスター発表会,研究発表会), 多田 雄哉, 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 22,   2006年10月27日, http://ci.nii.ac.jp/naid/110006212576
  • A-08 海洋細菌群集の構造と機能(I) : BrdU magnetic beads immunocapture and PCR-DGGE(BUMP-DGGE)法による解析(群集構造解析,口頭発表), 谷口 亮人, 多田 雄哉, 浜崎 恒二, 日本微生物生態学会講演要旨集, 21,   2005年10月30日, http://ci.nii.ac.jp/naid/110006320503
  • A-09 海洋細菌群集の構造と機能(II) : BrdU immunocytochemistry-FISH(BIC-FISH)法による解析(群集構造解析,口頭発表), 多田 雄哉, 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 21,   2005年10月30日, http://ci.nii.ac.jp/naid/110006320504
  • B-25 海洋環境における活性細菌群集の多様性解析2(群集構造解析,口頭発表), 谷口 亮人, 多田 雄哉, 浜崎 恒二, 日本微生物生態学会講演要旨集, 20,   2004年11月21日, http://ci.nii.ac.jp/naid/110006320280
  • B-26 BrdU immunocytochemistry-FISH(BIC-FISH)法の開発と海洋細菌群集構造解析への適用(群集構造解析,口頭発表), 多田 雄哉, 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 20,   2004年11月21日, http://ci.nii.ac.jp/naid/110006320281
  • 28-A-05 海洋環境における活性細菌群集の多様性解析(群集構造解析,一般講演), 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 19,   2003年10月26日, http://ci.nii.ac.jp/naid/110006320041
  • 28-C-01 有毒植物プランクトンに共生する細菌の探索(共生・相互作用,一般講演), 御手洗 由香, 西村 崇之, 谷口 亮人, 浜崎 恒二, 日本微生物生態学会講演要旨集, 19,   2003年10月26日, http://ci.nii.ac.jp/naid/110006320061

競争的資金

  • 文部科学省, 科学研究費補助金(若手研究(B)), クロマグロ養殖場海域の物質循環を統べる細菌鍵種の特定, 谷口 亮人, 近畿大学奄美実験場のクロマグロ養殖場では、サンゴが群棲している。環境に負荷を与える魚類養殖ときれいな海の象徴であるサンゴが共存しているこの事実は、至妙な物質循環が成立していることを示唆する。本研究では、サンゴが放出する粘液に注目して、この物質循環を支える細菌を解析した。具体的には、海水試料、ならびにサンゴ粘液をシリンジで直接採取した試料(粘液試料)および海水試料と粘液試料を混合した試料(混合試料)を用意した。平成25年度は、既得試料(平成21~24年5月および10月)と新規採取試料(平成25年5月および10月)の試料において、細菌群集構造を解析した。さらに、非養殖場海域における試料(既得試料)においても、細菌種の特定を行った。本研究では、増殖活性のある細菌群のみを標的にすることができるブロモデオキシウリジンを用いたDNAトレーサー法と、多様性解析手法の一つであるリボソーム遺伝子間転写領域自動解析あるいはCARD-FISH法を組み合わせて、サンゴ粘液によって増殖してくる細菌群の動態を明らかにした。現在のところ、粘液試料および混合試料においてのみ活発に増殖していた細菌を11種を特定した。これらのうち、6種はもともとの全体の細菌群では検出されなかった。一方で、粘液が加わると増殖活性がなくなる細菌種が9種存在した。活発に増殖している細菌の組成はAlphaproteobacteriaとBacteroidetesに属する細菌系統群で半分以上が占められていた。しかし、同じAlphaproteobacteriaに属していても、サンゴ粘液によって活発に増殖する系統群とそうでない系統群が存在していた。本研究で対象とする養殖場域では、AlphaproteobacteriaやBacteroidetesに属する系統群が重要な細菌群であることを示唆する。

教育活動情報

担当経験のある科目

  • 微生物海洋学特論, 近畿大学大学院農学研究科
  • 技術者倫理, 近畿大学農学部
  • 微生物海洋学, 近畿大学農学部