KINDAI UNIVERSITY


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伊原 誠イハラ マコト

プロフィール

所属部署名農学部 応用生命化学科 / 農学研究科
職名講師
学位博士(農学)
専門農薬化学
ジャンル経営・産業/農林
コメンテータガイドhttp://www.kindai.ac.jp/meikan/164-ihara-makoto.html
ホームページURLhttp://nara-kindai.unv.jp/02gakka/03ouyou/seigyo-lab/index.html
メールアドレス
Last Updated :2017/09/15

コミュニケーション情報 byコメンテータガイド

コメント

    イオンチャネルを標的とする農薬の作用機構を研究しています。特に、ネオニコチノイド剤の昆虫選択的毒性発現の分子機構を、構造生理学的観点から重点的に解析しています。

学歴・経歴

学歴

  •  - 2000年, 近畿大学, 農学部, 農芸化学科
  •  - 2002年, 近畿大学, 農学研究科, 応用生命化学専攻
  •  - 2005年, 近畿大学, 農学研究科, 応用生命化学専攻

経歴

  •   2005年04月,  - 2006年09月, 近畿大学 研究員
  •   2006年10月,  - 2012年11月, 独立行政法人 理化学研究所 播磨研究所放射光科学総合研究センター 研究員
  •   2012年12月,  - 2013年03月, 岡山大学大学院医歯薬学総合研究科(薬学系)(助教)
  •   2012年12月,  - 現在, 独立行政法人理化学研究所(客員研究員)
  •   2013年04月,  - 現在, 近畿大学(講師)

研究活動情報

研究分野

  • 情報学, 生体生命情報学
  • 生物分子科学, 生物分子科学
  • 生物科学, 構造生物化学
  • 生物科学, 機能生物化学
  • 農芸化学, 生物生産化学・生物有機化学

研究キーワード

  • 構造生理学

論文

  • An L319F mutation in transmembrane region 3 (TM3) selectively reduces sensitivity to okaramine B of the Bombyx mori l-glutamate-gated chloride channel,   2017年08月, 査読有り
  • Okaramine insecticidal alkaloids show similar activity on both exon 3c and exon 3b variants of glutamate-gated chloride channels of the larval silkworm, Bombyx mori, Furutani S, Ihara M, Kai K, Tanaka K, Sattelle D.B, Hayashi H, Matsuda K., NeuroToxicology, 60, 240, 244,   2017年, 査読有り
  • Ivermectin modulation of pH-sensitive chloride channels in the silkworm larvae of Bombyx mori., Nakatani Y, Furutani S, Ihara M, Matsuda K, Pesticide biochemistry and physiology, 126, 1, 5,   2016年01月, 査読有り
  • Comparative ecotoxicity of imidacloprid and dinotefuran to aquatic insects in rice mesocosms, Kobashi K, Harada T, Adachi Y, Mori M, Ihara M, Hayasaka D., Ecotoxicology and Environmental Safety, 138, 122, 129,   2017年, 査読有り
  • Selective regulation of pyrethrin biosynthesis by the specific blend of wound induced volatiles in Tanacetum cinerariifolium., Sakamori K, Ono N, Ihara M, Suzuki H, Matsuura H, Tanaka K, Ohta D, Kanaya S, Matsuda K, Plant signaling & behavior, 11, 4, e1149675,   2016年, 査読有り
  • Okaramine insecticidal alkaloids show similar activity on both exon 3c and exon 3b variants of glutamate-gated chloride channels of the larval silkworm, Bombyx mori., Furutani S, Ihara M, Kai K, Tanaka K, Sattelle DB, Hayashi H, Matsuda K, Neurotoxicology, 60, 240,   2016年05月, 査読有り
  • Loops D, E and G in the Drosophila Dα1 subunit contribute to high neonicotinoid sensitivity of Dα1-chicken β2 nicotinic acetylcholine receptor, Makoto Ihara, Mai Hikida, Hiroyuki Matsushita, Kyosuke Yamanaka, Yuya Kishimoto, Kazuki Kubo, Shun Watanabe, Mifumi Sakamoto, Koutaro Matsui, Akihiro Yamaguchi, Daiki Okuhara, Shogo Furutani, David B Sattelle, Kazuhiko Matsuda, Role of loops D, E and G in neonicotinoid actions,   2017年08月, 査読有り
  • Mechanisms of action, resistance and toxicity of insecticides targeting GABA receptors, Steven D. Buckingham, Makoto Ihara, David B Sattelle, Kazuhiko Matsuda, Current Medicinal Chemistry, 24,   2017年06月, 査読有り
  • Loops D, E and G in the Drosophila Dα1 subunit contribute to high neonicotinoid sensitivity of Dα1-chicken β2 nicotinic acetylcholine receptor, Makoto Ihara, Mai Hikida, Hiroyuki Matsushita, Kyosuke Yamanaka, Yuya Kishimoto, Kazuki Kubo, Shun Watanabe, Mifumi Sakamoto, Koutaro Matsui, Akihiro Yamaguchi, Daiki Okuhara, Shogo Furutani, David B Sattelle, Kazuhiko Matsuda, Role of loops D, E and G in neonicotinoid actions,   2017年06月, 査読有り
  • Modes of action, resistance and toxicity of insecticides targeting nicotinic acetylcholine receptors., Ihara M, Buckingham SD, Matsuda K, Sattelle DB, Current medicinal chemistry,   2017年02月, 査読有り
  • Ivermectin modulation of pH-sensitive chloride channels in the silkworm larvae of Bombyx mori, Nakatani Y, Furutani S, Ihara M, Matsuda K., Pesticide Biochemistry and Physiology, 126, 1, 5,   2016年, 査読有り
  • Probing new components (loop G and the α–α interface) of neonicotinoid binding sites on nicotinic acetylcholine receptors, Makoto Ihara, David B. Sattelle, Kazuhiko Matsud, Pesticide Biochemistry and Physiology, 121, 47, 52,   2015年02月, 査読有り
  • Meroterpenoid Chrodrimanins Are Selective and Potent Blockers of Insect GABA-Gated Chloride Channels, Yan Xu, Shogo Furutani, Makoto Ihara, Yun Ling, Xinling Yang, Kenji Kai, Hideo Hayashi, Kazuhiko Matsud, PLoS} {ONE, 10, 4, e0122629,   2015年04月, 査読有り
  • Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels., Furutani S, Ihara M, Nishino Y, Akamatsu M, Jones AK, Sattelle DB, Matsuda K, Molecular pharmacology, 86, 686, 695,   2014年12月, 査読有り
  • Studies on an Acetylcholine Binding Protein Identify a Basic Residue in Loop G on the  1 Strand as a New Structural Determinant of Neonicotinoid Actions, M. Ihara, T. Okajima, A. Yamashita, T. Oda, T. Asano, M. Matsui, D. B. Sattelle, K. Matsud, Molecular Pharmacology, 86, 6, 736,   2014年09月, 査読有り
  • GluCl a target of indole alkaloid okaramines: a 25 year enigma solved, Shogo Furutani, Yuri Nakatani, Yuka Miura, Makoto Ihara, Kenji Kai, Hideo Hayashi, Kazuhiko Matsud, Scientific Reports, 4, 6190,   2014年08月, 査読有り
  • A single amino acid polymorphism in the Drosophila melanogaster Dα1 (ALS) subunit enhances neonicotinoid efficacy at Dα1-chicken β2 hybrid nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes, Makoto Ihara, Naoya Shimazu, Mai Utsunomiya, Miki Akamatsu, David B. Sattelle, Kazuhiko Matsud, Bioscience, Biotechnology and Biochemistry, 78, 4, 543, 549,   2014年04月, 査読有り
  • General flexible nature of the cytosolic regions of fungal transient receptor potential (TRP) channels, revealed by expression screening using GFP-fusion techniques., Ihara M, Takano Y, Yamashita A, Protein science : a publication of the Protein Society,   2014年04月, 査読有り
  • Molecular Bases of Multimodal Regulation of a Fungal Transient Receptor Potential (TRP) Channel, Ihara M, Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A, Journal of Biological Chemistry, 288, 21, 15303, 15317,   2013年, 査読有り
  • GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs., Ashikawa Y, Ihara M, Matsuura N, Fukunaga Y, Kusakabe Y, Yamashita A, Protein science : a publication of the Protein Society, 20, 1720, 1734,   2011年10月, 査読有り
  • High-resolution Native-PAGE for membrane proteins capable of fluorescence detection and hydrodynamic state evaluation., Ihara M, Matsuura N, Yamashita A, Analytical biochemistry, 412, 2, 217, 223,   2011年05月, 査読有り
  • Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin., Ihara M, Okajima T, Yamashita A, Oda T, Hirata K, Nishiwaki H, Morimoto T, Akamatsu M, Ashikawa Y, Kuroda S, Mega R, Kuramitsu S, Sattelle DB, Matsuda K, Invertebrate neuroscience : IN, 8, 71, 81,   2008年06月, 査読有り
  • Potentiating and blocking actions of neonicotinoids on the response to acetylcholine of the neuronal α4β2 nicotinic acetylcholine receptor, TOSHIMA Kayoko, IHARA Makoto, KANAOKA Satoshi, TARUMOTO Kiyoshi, YAMADA Atsushi, SATTELLE David B., MATSUDA Kazuhiko, Journal of pesticide science = 日本農薬学会誌, Journal of pesticide science = 日本農薬学会誌, 33, 2, 146, 151,   2008年05月20日
  • Cy3-3-acylcholine: a fluorescent analogue of acetylcholine for single molecule detection., Fujimoto K, Yoshimura Y, Ihara M, Matsuda K, Takeuchi Y, Aoki T, Ide T, Bioorganic & medicinal chemistry letters, 18, 3, 1106, 1109,   2008年02月, 査読有り
  • Potentiating and blocking actions of neonicotinoids on the response to acetylcholine of the neuronal α4β2 nicotinic acetylcholine receptor, Toshima Kayoko, Ihara Makoto, Kanaoka Satoshi, Tarumoto Kiyoshi, Yamada Atsushi, Sattelle David B, Matsuda Kazuhik, Journal of Pesticide Science, 33, 2, 146, 151,   2008年, 査読有り
  • Blocking actions of alkylene-tethered bis-neonicotinoids on nicotinic acetylcholine receptors expressed by terminal abdominal ganglion neurons of Periplaneta americana., Ihara M, Hirata K, Ishida C, Kagabu S, Matsuda K, Neuroscience letters, 425, 137, 140,   2007年10月, 査読有り
  • 殺虫剤のリガンド作動性イオンチャネルに対する作用機構研究(学会賞受賞論文(奨励賞)), 伊原 誠, Journal of pesticide science = 日本農薬学会誌, Journal of pesticide science = 日本農薬学会誌, 32, 3, 283, 290,   2007年08月20日
  • Proinsecticide candidates N-(5-methyl-2-oxo-1,3-dioxol-4-yl)methyl derivatives of imidacloprid and 1-chlorothiazolylmethyl-2-nitroimino-imidazolidine., Ohno I, Hirata K, Ishida C, Ihara M, Matsuda K, Kagabu S, Bioorganic & medicinal chemistry letters, 17, 16, 4500, 4503,   2007年08月, 査読有り
  • A hypothesis to account for the selective and diverse actions of neonicotinoid insecticides at their molecular targets, nicotinic acetylcholine receptors: catch and release in hydrogen bond networks., Ihara M, Shimomura M, Ishida C, Nishiwaki H, Akamatsu M, Sattelle DB, Matsuda K, Invertebrate neuroscience : IN, 7, 47, 51,   2007年03月, 査読有り
  • Mechanisms of action of insecticides on ligand-gated ion channels, Ihara Makot, Journal of Pesticide Science, 32, 3, 278, 280,   2007年, 査読有り
  • Role in the selectivity of neonicotinoids of insect-specific basic residues in loop D of the nicotinic acetylcholine receptor agonist binding site., Shimomura M, Yokota M, Ihara M, Akamatsu M, Sattelle DB, Matsuda K, Molecular pharmacology, 70, 1255, 1263,   2006年10月, 査読有り
  • Neonicotinoid insecticides display partial and super agonist actions on native insect nicotinic acetylcholine receptors., Brown LA, Ihara M, Buckingham SD, Matsuda K, Sattelle DB, Journal of neurochemistry, 99, 2, 608, 615,   2006年10月, 査読有り
  • Isolation and identification of histamine-release inhibitors from Pistacia weinmannifolia J. Pisson ex. Franch, MINAMI Kumiko, NAKASUGI Toru, SUN Han-Dong, HOU Ai-Jun, IHARA Makoto, MORIMOTO Masanori, KOMAI Koichiro, Journal of natural medicines, Journal of natural medicines, 60, 2, 138, 140,   2006年05月, 査読有り
  • Actions of imidacloprid, clothianidin and related neonicotinoids on nicotinic acetylcholine receptors of American cockroach neurons and their relationships with insecticidal potency, Ihara Makoto, Brown Laurence A, Ishida Chiharu, Okuda Hiroshi, Sattelle David B, Matsuda Kazuhik, Journal of Pesticide Science, 31, 1, 35, 40,   2006年, 査読有り
  • Insect-vertebrate chimeric nicotinic acetylcholine receptors identify a region, loop B to the N-terminus of the Drosophila Dalpha2 subunit, which contributes to neonicotinoid sensitivity., Shimomura M, Satoh H, Yokota M, Ihara M, Matsuda K, Sattelle DB, Neuroscience letters, 385, 168, 172,   2005年09月, 査読有り
  • Differential blocking actions of 4′-ethynyl-4-n-propylbicycloorthobenzoate (EBOB) and γ-hexachlorocyclohexane (γ-HCH) on γ-aminobutyric acid- and glutamate-induced responses of American cockroach neurons, Ihara Makoto, Ishida Chiharu, Okuda Hiroshi, Ozoe Yoshihisa, Matsuda Kazuhik, Invertebrate Neuroscience, 5, 3-4, 157, 164,   2005年, 査読有り
  • Neonicotinoids Show Selective and Diverse Actions on Their Nicotinic Receptor Targets: Electrophysiology, Molecular Biology, and Receptor Modeling Studies, MATSUDA Kazuhiko, SHIMOMURA Masaru, IHARA Makoto, AKAMATSU Miki, SATTELLE David B., Bioscience, Biotechnology, and Biochemistry, Bioscience, Biotechnology, and Biochemistry, 69, 8, 1442, 1452,   2005年, 査読有り
    概要:Neonicotinoid insecticides, which act selectively on insect nicotinic acetylcholine receptors (nAChRs), are used worldwide for insect pest management. Studies that span chemistry, biochemistry, molecular biology, and electrophysiology have contributed to our current understanding of the important physicochemical and structural properties essential for neonicotinoid actions as well as key receptor residues contributing to the high affinity of neonicotinoids for insect nAChRs. Research to date suggests that electrostatic interactions and possibly hydrogen bond formation between neonicotinoids...
  • Super Agonist Actions of Clothianidin and Related Compounds on the SADβ2 Nicotinic Acetylcholine Receptor Expressed in Xenopus laevis Oocytes, IHARA Makoto, MATSUDA Kazuhiko, SHIMOMURA Masaru, SATTELLE David B., KOMAI Koichiro, Bioscience, biotechnology, and biochemistry, Bioscience, biotechnology, and biochemistry, 68, 3, 761, 763,   2004年03月, 査読有り
    概要:To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken β2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electrophysiology. The maximum response of the SADβ2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist ...
  • Action of nereistoxin on recombinant neuronal nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes., Raymond Delpech V, Ihara M, Coddou C, Matsuda K, Sattelle DB, Invertebrate neuroscience : IN, 5, 29, 35,   2003年11月, 査読有り
  • Insecticidal and Neural Activities of Candidate Photoaffinity Probes for Neonicotinoid Binding Sites(Organic Chemistry), MATSUDA Kazuhiko, IHARA Makoto, NISHIMURA Keiichiro, SATTELLE David B., KOMAI Koichiro, Bioscience, biotechnology, and biochemistry, Bioscience, biotechnology, and biochemistry, 65, 7, 1534, 1541,   2001年07月, 査読有り
    概要:Photoreactive derivatives of imidacloprid and its nitromethylene analogue were synthesized as candidate photoaffinity probes for identifying the amino acid residues of nicotinic acetylcholine receptors (nAChRs) that interact with the neonicotinoid insecticides. When the candidate probes were injected into American cockroaches, the nerve cord neural activity initially increased, then ceased and death of the insect followed. Both the nerve cord and toxicity were enhanced by changing the photoreactive substituent from the para position to the meta position on the spacer benzyl moiety. When tes...
  • Role of loop D of the α7 nicotinic acetylcholine receptor in its interaction with the insecticide imidacloprid and related neonicotinoids, Matsuda Kazuhiko, Shimomura Masaru, Kondo Yumi, Ihara Makoto, Hashigami Kaori, Yoshida Naofumi, Raymond Valérie, Mongan Nigel P, Freeman John C, Komai Koichiro, et al, British Journal of Pharmacology, 130, 5, 981, 986,   2000年, 査読有り

MISC

  • TRPチャネルがさまざまな刺激に応答できる仕組み, 伊原誠, 山下敦子, 化学と生物, 52, 1, 48, 53,   2014年01月01日, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402235189697790
  • クローズアップ実験法 Series218 FN‐PAGE法:膜タンパク質の性状の簡便かつ迅速な解析法, 伊原誠, 山下敦子, 実験医学, 29, 14, 2291, 2297,   2011年09月01日, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102262575470704

受賞

  •   2007年, 日本農薬学会 奨励賞
  •   2007年, 望月喜多司記念賞 奨励賞
  •   2008年, 日本農学進歩賞

教育活動情報

担当経験のある科目

  • 生命情報学, 近畿大学
  • 生命情報学実習, 近畿大学