神山 匡 (カミヤマ タダシ)

  • 理工学部 理学科 教授/学科長/主任
Last Updated :2024/03/24

コミュニケーション情報 byコメンテータガイド

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    タンパク質の機能発現に重要な立体構造や分子認識を熱力学的に研究しています。特に温度、圧力、溶媒、添加物の効果についてです。
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研究者情報

学位

  • 博士(理学)(広島大学)

ホームページURL

J-Global ID

研究キーワード

  • 熱測定   圧縮率   タンパク質   物理化学   Physical chemistry   

現在の研究分野(キーワード)

    タンパク質の機能発現に重要な立体構造や分子認識を熱力学的に研究しています。特に温度、圧力、溶媒、添加物の効果についてです。

研究分野

  • ナノテク・材料 / 生体化学
  • ナノテク・材料 / 基礎物理化学

経歴

  • 2000年 - 2001年  広島大学ベンチャー・ビジネス・ラボラトリー中核的研究機関 研究員
  • 2000年 - 2001年  Hiroshima University Venture Business Laboratory, Post Doctoral Fellow

学歴

  •         - 2000年   広島大学大学院   理学研究科   遺伝子科学
  •         - 2000年   広島大学大学院   Graduate School, Division of Natural Science
  •         - 1995年   広島大学   理学部   物性
  •         - 1995年   広島大学   Faculty of Science

所属学協会

  • イオン液体研究会   溶液化学研究会   生物物理学会   日本化学会   シクロデキストリン学会   蛋白質科学会   日本熱測定学会   

研究活動情報

論文

  • Taro Yamamoto; Tomonari Wakabayashi; Tadashi Kamiyama; Hal Suzuki
    Thermochimica Acta 730 179629 - 179629 2023年12月 [査読有り]
  • Taro Yamamoto; Yuki Nagae; Tomonari Wakabayashi; Tadashi Kamiyama; Hal Suzuki
    Soft matter 19 8 1492 - 1498 2023年02月 [査読有り]
     
    A differential scanning calorimeter equipped with a shearing system (shear rate of  < 400 s-1) was developed to elucidate the thermodynamic properties of liquid crystalline phase transitions under shear flow. An analytical method was proposed to accurately estimate the heat flow caused by shear friction to evaluate the transition entropies. The phase transitions of 4'-n-octyl-4-cyano-biphenyl (8CB) under shear flow were investigated using the developed calorimeter. Although several shear-induced transitions for 8CB have been reported in the past using viscosity and small-angle X-ray scattering (SAXS) measurements, only the nematic-isotropic (N-I) and smectic-A-nematic (SA-N) transitions were detected as heat flow peaks. The N-I transition temperature was almost independent of the shear rate. The SA-N transition temperature was also independent of the shear rate, but the transition peak was broadened by applying shear flow. For both transitions, the transition entropies were independent of the shear rate. These results suggest that the thermodynamic properties were not considerably changed by shearing because the molecular alignments in the domains were not substantially changed, whereas shearing changed the LC domain directions, which can be detected by viscosity and SAXS measurements.
  • Miyu Umeda; Tomonari Wakabayashi; Tadashi Kamiyama; Hal Suzuki
    Polymer 254 125105 - 125105 2022年07月 [査読有り]
  • Taro Yamamoto; Yusuke Yagi; Toshimitsu Hatakeyama; Tomonari Wakabayashi; Tadashi Kamiyama; Hal Suzuki
    Colloids and Surfaces A: Physicochemical and Engineering Aspects 625 126859 - 126859 2021年09月 [査読有り]
  • 神山 匡
    熱測定 47 1 8 - 13 2020年01月 [査読有り]
  • Thermodynamics and kinetics of aggregation of myoglobin with 1,4- dioxane
    神山 匡
    Netsu sokutei 45 2 63 - 69 2018年04月 [査読有り]
  • Tomokadu Marutani; Takashi Inomata; Tadashi Kamiyama
    Thermochimica Acta 659 8 - 12 2018年01月 [査読有り]
     
    The thermodynamic properties and phase diagram of the thermal-induced fibrous aggregation of cytochrome c with a simple organic co-solvent, 1,4-dioxane, were determined by differential scanning calorimetry, circular dichroism, and scanning electron microscopy at various concentrations of 1,4-dioxane. At x = 0.2–0.3 mol fraction of 1,4-dioxane, cytochrome c transformed to an alpha helix-rich denatured state at 25 °C, which transformed into a fibrous aggregation upon heating. The enthalpy change for the fibrous aggregation, −456 kJ/mol (x = 0.30), was numerically greater than the thermal denaturation of the native state, 298 kJ/mol (x = 0), indicating that aggregate formation involved a conformation with many intermolecular interactions. At x > 0.3, cytochrome c aggregated and precipitated at 25 °C without heating, and the precipitate transformed into an amorphous state. These results suggest that the previous state is important for transition into the fibrous state. Thermodynamic data on the use of co-solvents for globular proteins will facilitate understanding of the aggregation mechanism.
  • Tadashi Kamiyama; Tomokadu Marutani; Dai Kato; Takuya Hamada; Keiichi Kato; Takayoshi Kimura
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 123 3 1861 - 1869 2016年03月 [査読有り]
     
    To reveal the contribution of hydrophobic interactions to the stability of the molten globule (MG) state of proteins, the effect of sorbitol on the structure of acid-unfolded (AU) equine heart myoglobin was examined at pH 2 by means of circular dichroism (CD), stopped-flow CD, rheometry, and differential scanning calorimetry. The AU state of myoglobin was refolded by adding sorbitol to the MG state, which had a secondary structure and hydrodynamic volume similar to the native (N) state. The thermal denaturation of the MG state showed considerably small enthalpy change, low cooperativity, and small heat capacity compared to the N state unlike MG state of cytochrome c, indicating that the presence of the heme is important to preserve the strict tertiary structure of MG state not only N state, for heme proteins. The refolding was induced by preferential exclusion of three sorbitol molecules from the AU state compared to the MG state. The transition from the N-MG state kinetically proceeded via the AU state, followed by gradual refolding due to the preferential exclusion of sorbitol with 1.98 x 10(-3) s(-1) of kinetic constant at 3.3 M sorbitol and 10.9 A degrees C.
  • Kunisato Kuroi; Koji Okajima; Masahiko Ikeuchi; Satoru Tokutomi; Tadashi Kamiyama; Masahide Terazima
    JOURNAL OF PHYSICAL CHEMISTRY B 119 7 2897 - 2907 2015年02月 [査読有り]
     
    The effect of pressure on the dissociation reaction of the TePixD decamer was investigated by high-pressure transient grating (TG). The TG signal intensity representing the dissociation reaction of the TePixD decamer significantly decreased by applying a relatively small pressure. On the other hand, the reaction rate increased with increasing pressure. The equilibrium between the pentamer and the decamer was investigated by high-pressure dynamic light scattering; The results indicated that the fraction of the decamer slightly increased in the high-pressure region: From these measurements, it was concluded that the pressure-dependent signal intensity originated from the decrease of the quantum yield of the dissociation reaction of the decamer, indicating that this reaction efficiency is very sensitive to pressure. Using densimetry at high pressures, the compressibility was found to be pressure dependent even in a relatively low pressure range. We attributed the origin of the pressure-sensitive reaction yield to the decrease of compressibility at high pressure. Because the compressibility is related to the volume fluctuation, this observation suggests that the driving force for this reaction is fluctuation of the protein. The relationship between the cavities at the interfaces of the monomer units and the reactivity is also discussed.
  • Takayoshi Kimura; Tasunori Suzuki; Keisuke Takata; Akina Soga; Yutsa Nomoto; Tadashi Kamiyama; Yousuke Nakai; Hideo Matsui; Masao Fujisawa
    Journal of Thermal Analysis and Calorimetry 113 3 1467 - 1474 2013年09月 [査読有り]
     
    The molar excess enthalpies of eight systems of butylamines + propanols were determined at 298.15 K using a twin-microcalorimeter. All excess enthalpies were exothermic and large. An equilibrium constant K 1 expressed in terms of mole fractions and standard thermodynamic properties of formation (Δf H, Δf G, Δf S) of 1:1 complex were evaluated by ideal mixtures of monomeric molecules and their associated complexes. Concentration dependence of the FT-Raman spectrum showed systematic changes of bands. Spectroscopic considerations based on this and ab initio calculations on molecules were performed at the Mp2/6-311G(d,p) level of theory. Interaction energies between butylamine and propanol were calculated by the supermolecular and NBO methods. The results were discussed with previous results to clarify the steric and positional effect of the amino and hydroxyl group. © 2013 Akadémiai Kiadó, Budapest, Hungary.
  • 神山 匡; 木村 隆良
    Journal of Thermal Analysis and Calorimetry 113 3 1491 - 1496 Springer 2013年09月 [査読有り]
     
    種々の修飾化βシクロデキストリンによるシトクロムcの不安定化機構をDSCを用いて熱力学的に明らかにした。
  • 神山 匡
    熱測定 39 2 47 - 53 日本熱測定学会 2012年03月 [査読有り]
  • Tadashi Kamiyama; Megumi Satoh; Takahiro Tateishi; Tomoaki Nojiri; Daisuke Takeuchi; Takayoshi Kimura
    THERMOCHIMICA ACTA 532 10 - 14 2012年03月 [査読有り]
     
    Effects of cyclic oligosaccharide cyclodextrin (CD) on stability and conformation of lysozyme were clarified thermodynamically and rheologically by DSC, viscosity, and circular dichroism measurements. The modified beta-CD relatively destabilized the folded state of lysozyme by stabilizing the unfolded state due to inclusion of hydrophobic part into the hydrophobic interior of CD. The order of higher destabilization effect was acetyl-beta-CD > methyl-beta-CD > hydroxypropyl-beta-CD. Apparent number of bound CD to unfolded state for methyl-, hydroxypropyl-, and acetyl-beta-CD is 6.7 +/- 0.7, 4.2 +/- 1.1, and 18.6 +/- 4.3 and the binding constant is 5.5 +/- 0.8, 6.7 +/- 2.4, and 4.4 +/- 1.2 Lmol(-1), respectively. The viscosity for unfolded state was increased with an increase in the each modified beta-CD concentration, suggesting that the inclusion of CD on a part of hydrophobic core at unfolded state leads to break the hydrophobic core, then lysozyme would be more spread structure. The substituent of CD can accelerate instability by directly breaking hydrogen bond and/or can restrain instability by increase in hydrophobic interaction. The fact that the each modified CDs has different destabilization effect shows a possibility to control the stability of protein by the substitution of CD. (C) 2011 Elsevier B.V. All rights reserved.
  • Shigefumi Mimura; Takahisa Yamato; Tadashi Kamiyama; Kunihiko Gekko
    BIOPHYSICAL CHEMISTRY 161 39 - 45 2012年02月 [査読有り]
     
    The evolution of structural fluctuations of proteins was examined by calculating the isothermal compressibility (beta(T)) values of chicken lysozyme and its six evolutionary mutants at Thr40, Ile55, and Ser91 (a ternary mutant corresponding to bobwhite lysozyme) from their X-ray structures by normal-mode analysis at 300 K. The Or values of the two extant lysozymes from chicken and bobwhite were 1.61 and 1.59 Mbar(-1), respectively, but five other evolutionary mutants showed larger beta(T) values of up to 2.17 Mbar(-1). These results suggest that ancestral lysozymes exhibit larger volume fluctuations than extant ones, and hence that the molecular evolution of lysozymes has followed a nonneutral evolutionary pathway. The evolutionary mutants contained large amount of cavities, although no change was visible in the X-ray structures. There was a linear correlation between beta(T) and total cavity volume, predicting that the cavity volume or atomic packing is an important factor regulating volume fluctuations during the molecular evolution of this protein. (C) 2011 Elsevier B.V. All rights reserved.
  • Effects of cyclodextrin on thermal stability of cytochrome c
    神山 匡
    Netsusokutei W39 1 - 5 2012年 [査読有り]
  • Takayoshi Kimura; Satoshi Fujie; Takashi Yukiyama; Masao Fujisawa; Tadashi Kamiyama; Hatsumi Aki
    Journal of Inclusion Phenomena and Macrocyclic Chemistry 70 3-4 269 - 278 2011年08月 [査読有り]
     
    In order to investigate the contribution of the hydrophilic parts of guest molecules of aliphatic compounds to the inclusion reaction, the thermodynamic properties of inclusion compounds of cyclodextrin (α-CD) with eight pentane derivatives into the cavity of α-CD in dilute aqueous solutions were measured by micro-calorimeter at 298.15 K. The thermodynamic properties of the inclusion reactions of pentane derivatives into α-CD were compared to the hydrophobicity and polarizability of guest molecules and discussed. The interaction energies of inclusion compounds of α-CD and pentane derivatives were determined by DFT calculation (B3LYP/6-31G (d,p)) in water and compared with the experimental results. © 2010 Springer Science+Business Media B.V.
  • Takayoshi Kimura; Satoshi Fujie; Takashi Yukiyama; Masao Fujisawa; Tadashi Kamiyama; Hatsumi Aki
    JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY 70 3-4 269 - 278 2011年08月 [査読有り]
     
    In order to investigate the contribution of the hydrophilic parts of guest molecules of aliphatic compounds to the inclusion reaction, the thermodynamic properties of inclusion compounds of cyclodextrin (alpha-CD) with eight pentane derivatives into the cavity of alpha-CD in dilute aqueous solutions were measured by micro-calorimeter at 298.15 K. The thermodynamic properties of the inclusion reactions of pentane derivatives into alpha-CD were compared to the hydrophobicity and polarizability of guest molecules and discussed. The interaction energies of inclusion compounds of alpha-CD and pentane derivatives were determined by DFT calculation (B3LYP/6-31G (d,p)) in water and compared with the experimental results.
  • Hongli Liu; Satoko Kido; Tadashi Kamiyama; Masao Fujisawa; Takayoshi Kimura
    JOURNAL OF CHEMICAL THERMODYNAMICS 43 4 627 - 634 2011年04月 
    Excess enthalpies for binary mixtures (S-fenchone + ethanol/benzene/cyclohexane/carbon tetrachloride) were measured over the whole concentration at T = 298.15 K. The experimental results were compared with the values obtained from the UNIFAC, COSMO-RS and regular solution theory. Excess enthalpies of binary mixtures of R-fenchone and S-fenchone in ethanol, benzene, and cyclohexane solution at different specified mole fractions of fenchone have been measured under the same conditions. With the decreasing of the specified mole fraction of fenchone in different solutions, the excess enthalpies of mixing of chiral orientated solutions increased and became close to zero. Results were compared with those of chiral limonene in ethanol solution. Pair interaction energies were also investigated. (C) 2010 Elsevier Ltd. All rights reserved.
  • Takayoshi Kimura; Satoko Kido; Tadashi Kamiyama; Masao Fujisawa
    CHIRALITY 23 1E E98 - E104 2011年 [査読有り]
     
    Enthalpies of mixing of R- and S-limonene in non-polar solvents in the entire range of mole fractions were measured at 298.15 K. The enthalpies of mixing were negative for all concentrations in dilute concentration, but increased by increasing the concentration of limonenes in solutions. Ultimately positive excess enthalpies were shown in high concentration. Enthalpies of mixing were compared with theoretical estimation by COSMO-RS. Chirality 23: E98-E104, 2011. (C) 2011 Wiley Periodicals, Inc.
  • 木村 隆良; 劉 紅麗; 木戸覚子; 神山 匡; 藤澤 雅夫
    Journal of Thermal Analysis and Calorimetry 102 3 1123 - 1133 Springer 2010年12月 
    メトキシメチルベンゼン、エチルメチルベンゼン、ジエチルベンゼン、クロロメチルベンゼン、トルニトリル、フルオロベンゾニトリル、トリルアセトニトリルの(o + m), (o + p), (m + p)-異性体系の過剰エンタルピーを測定し、他の構造異性多系の結果と比較した。また超分子法による非経験分子軌道計算から相互作用エネルギーを決定し、実測値と比較検討した。(英語)
  • 神山 匡
    物性研究 95 3 317 - 319 物性研究刊行会 2010年12月
  • 神山 匡
    JASCO Report 52 2 16 - 19 JASCO 2010年10月 
    CDの添加はリゾチームの熱安定性を減少させ、その不安定化効果はCDの内径とリゾチームとのモル比に依存していた。これらの結果からCDによるリゾチームの不安定化がCDとリゾチームの直接的な相互作用に起因しており、変性よって溶媒に露出した疎水性残基をCDが包接することによって変性状態の安定性を高め、相対的に天然状態を不安定化しているものと思われる。本研究で示した結果に加え、現在、異なる修飾基の影響や流体力学的な効果についても研究を進めている。より詳細な不安定化のメカニズムの解明にはDSCによる熱力学的な解析やNMRなどの微視的情報が必要となるが、円二色性とペルチェ式恒温システムを用いることにより、タンパク質の温度による構造変化を容易に観測することが可能になり、タンパク質の構造や安定性に及ぼす添加物の効果を見積もることができる。
  • 木村 隆良; 劉 紅麗; 木戸覚子; 神山 匡; 藤澤 雅夫
    熱測定 37 3 96 - 103 日本熱測定学会 2010年09月 
    o-キシレンと12個のo-異性体(エチルトルエン,メトキシトルエン,フルオロトルエン, クロロトルエン, ブロモトルエン,ヨードトルエン,トリルアセトニトリル, フルオロイオドベンゼン,ブロモフルオロベンゼン,クロロフルオロベンゼン,フルオロニトロベンゼン,アミノフルオロベンゼン)系について全濃度範囲の過剰エンタルピーを決定した。比較的大きな吸熱を示したフルオロニトロトルエン系およびアミノフルオロトルエン系を除いて小さなエンタルピー変化を示した。 UNIFAC、密度汎関数COSMO-RS、正則溶液論による推算値と比較した結果、UNFACの結果は実測値から大きく正に外れたが密度汎関数COSMO-RSが最もよく一致した。Keesom-Israelachvili 式によって過剰エンタルピーと双極子相互作用の効果を検討した(英語)
  • Hongli Liu; Tomoko Yamashita; Tadashi Kamiyama; Masao Fujisawa; Takayoshi Kimura
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 99 1 95 - 103 2010年01月 [査読有り]
     
    The excess molar enthalpies of 8 binary mixtures for the o-, m-, and p-isomers of fluoroiodobenzene, fluoromethoxybenzene, bromofluorobenzene, chlorofluoro-benzene, difluorobenzene, fluoromethylbenzene, fluoronitrobenzene, and aminofluoro-benzene were measured at 298.15 K. The changes of the measured enthalpies were very small. The experimental results revealed that the isomers containing two electron-acceptor groups showed the most positive excess enthalpy change, while isomers containing both one electron donor and one electron acceptor group, such as aminofluorobenzene, showed more stable and always the most negative results.
  • 二成分溶液中(水+DMSO)におけるリゾチームの熱力学
    神山 匡
    熱測定 36 5 263 - 270 日本熱測定学会 2009年12月 
    二成分溶液中(水+DMSO)におけるリゾチームの熱力学的特性を固有粘度,部分比容,粘性流動に対する活性化自由エネルギー,DSC測定を指標にして明らかにした。リゾチームはDMSO低濃度(x2 =0.05)において安定化を示し,x2=0.3 ~0.4 における構造崩壊に伴い,[h],vo,DμO*3が増加した。二成分溶液での過剰熱力学量に反映されるように水‐DMSO間の強い水素結合により水‐リゾチームの相互作用が影響を受け構造転移に至ったものと思われる。また,見かけのリゾチームの部分比容はリゾチームとDMSO濃度に大きく依存しており,選択的な水和や溶媒和の存在を示唆している。これらの結果は二成分溶液中のタンパク質の熱力学量が構造崩壊に伴うタンパク質のパッキングや表面積変化に伴う溶媒との相互作用変化を敏感に反映していることを示している。
  • Kunihiko Gekko; Mariko Araga; Tadashi Kamiyama; Eiji Ohmae; Kazuyuki Akasaka
    BIOPHYSICAL CHEMISTRY 144 1-2 67 - 71 2009年09月 
    There are some theoretical arguments related to interpreting the adiabatic compressibility (beta(s)) of a protein determined from the sound velocity and the difference between beta(s) and isothermal compressibility (beta(T)). To address these problems experimentally, we constructed a high-pressure oscillating densitometer and used it to measure the apparent specific volume of bovine serum albumin as a function of pressure (0.1-78 MPa) and temperature (5-35 degrees C). The beta(T) determined from plots of the apparent specific volume vs. pressure was slightly larger than beta(s) at all temperatures examined, with the difference between the two compressibilities increasing as the temperature was decreased. Only at room temperature did the observed beta(T) agree with those estimated from beta(s) using the heat capacity and the thermal expansibility of the protein, suggesting that there are significant as-yet-unknown mechanisms that affect protein compressibility. (C) 2009 Elsevier B.V. All rights reserved.
  • T. Kamiyama; H. L. Liu; T. Kimura
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 95 2 353 - 359 2009年02月 
    To reveal the denaturation mechanism of lysozyme by dimethyl sulfoxide (DMSO), thermal stability of lysozyme and its preferential solvation by DMSO in binary solutions of water and DMSO was studied by differential scanning calorimetry (DSC) and using densities of ternary solutions of water (1), DMSO (2) and lysozyme (3) at 298.15 K. A significant endothermic peak was observed in binary solutions of water and DMSO except for a solution with a mole fraction of DMSO (x (2)) of 0.4. As x (2) was increased, the thermal denaturation temperature T (m) decreased, but significant increases in changes in enthalpy and heat capacity for denaturation, Delta H (cal) and Delta C (p), were observed at low x (2) before decreasing. The obtained amount of preferential solvation of lysozyme by DMSO (a,g (2)/a,g (3)) was about 0.09 g g(-1) at low x (2), indicating that DMSO molecules preferentially solvate lysozyme at low x (2). In solutions with high x (2), the amount of preferential solvation (a,g (2)/a,g (3)) decreased to negative values when lysozyme was denatured. These results indicated that DMSO molecules do not interact directly with lysozyme as denaturants such as guanidine hydrochloride and urea do. The DMSO molecules interact indirectly with lysozyme leading to denaturation, probably due to a strong interaction between water and DMSO molecules.
  • Kristian Boehm; Jessica Guddorf; Alexander Albers; Tadashi Kamiyama; Susanne Fetzner; H. -J. Hinz
    BIOCHEMISTRY 47 27 7116 - 7126 2008年07月 
    Thermodynamic stability parameters and the equilibrium unfolding mechanism of HiS(6)HodC69S, a mutant of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (Hod) having a Cys to Ser exchange at position 69 and an N-terminal hexahistidine tag (HiS(6)HodC69S), have been derived from isothermal unfolding studies using guanidine hydrochloride (GdnHCl) or urea as denaturants. The conformational changes were monitored by following changes in circular dichroism (CD), fluorescence, and dynamic light scattering (DLS), and the resulting transition curves were analyzed on the basis of a sequential three-state model N = I = D. The structural changes have been correlated to catalytic activity, and the contribution to stability of the disulfide bond between residues C37 and C184 in the native protein has been established. A prominent result of the present study is the finding that, independent of the method used for denaturing the protein, the unfolding mechanism always comprises three states which can be characterized by, within error limits, identical sets of thermodynamic parameters. Apparent deviations from three-state unfolding can be rationalized by the inability of a spectroscopic probe to discriminate clearly between native, intermediate, and unfolded ensembles. This was the case for the CD-monitored urea unfolding curve.
  • 藤澤 雅夫; 木村 隆良; 中野雄仁; 神山 匡; 大津隆行; 前田宗; 高木定夫
    Journal of Thermal Analysis and Calorimetry 90 2 581 - 585 Springer 2007年11月 
    数種のアルコールのα-シクロデキストリン空洞への包接化エンタルピーを測定し、分子力学法によって決定した溶媒和エンタルピーとの相関を議論した(英文)。
  • T. Kimura; Y. Takahashi; T. Kamiyama; M. Fujisawa
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 88 2 587 - 595 2007年05月 
    Excess enthalpies (H-E) of 17 binary mixtures of o- and m-isomers of dichlorobenzene, difluorobenzene, methoxymethylbenzene, dimethylbenzene, dimethoxybenzene, aminofluorobenzene, fluoronitrobenzene, diethylbenzene, chlorofluorobenzene, fluoroiodobenzene, bromofluorobenzene, chloromethylbenzene, fluoromethylbenzene, bromomethylbenzene, iodomethylbenzene, fluoromethoxybenzene, dibromobenzene at 298.15 K were measured. All excess enthalpies measured were very small, and those of o-+m-isomers of aminofluorobenzene, dibromobenzene and iodomethylbenzene were negative but 14 other binary mixtures of isomers were positive over the whole range of mole fractions. H-E of o-+m-isomers of dimethoxybenzene showed the largest enthalpic instability and those of aminofluorobenzene showed the largest enthalpic stability. There was a correlation between dipole-dipole interaction, dipole-induced dipole interaction or entropies of vaporization and excess partial molar enthalpies at infinite dilution.
  • T. Kimura; T. Kitai; T. Kamiyama; A. Fujisawa
    THERMOCHIMICA ACTA 450 1-2 91 - 95 2006年11月 
    The molar excess enthalpies of 1,2- and 1,3-propanediamine + 1 - or 2-propanol and 1,2- and 1,3-propanediol + 1- or 2-propaneamine have been determined at 298.15 K using a twin-microcalorimeter for a series of runs over the whole range of mole fractions. All excess enthalpies were large exothermic, in particular, the systems of amines + propanediols were more than -5 kJ mol(-1) at the minimum. Primary or secondary alcohols and an-tines showed systematically different enthalpic behaviors. Equilibrium constant K1 expressed in terms of mole fractions and standard enthalpy of the formation of a 1: 1 complex have been evaluated by ideal mixtures of momomeric molecules and their associated complexes. (c) 2006 Elsevier B.V. All rights reserved.
  • T. Kimura; M. A. Khan; T. Kamiyama
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 85 3 559 - 565 2006年09月 
    Enthalpies of mixing of R- and S-enantiomers of dicarboxylic acids such as 2-methylbutanedioic acid (MBA), 2-hydroxylbutanedioic acid (HBA), 2-methylpentanedioic acid (MPA) and 2-hydroxyl-2-methylbutanedioic acid (HMBA) in ethanol solution have been measured for a large range of mole fraction of heterochiral dicarboxylic acid at 298.15 K. Also densities of ethanol solution of the dicarboxylic acids were determined. Enthalpies of mixing were exothermic for all the concentrations. Enthalpic stabilization on mixing was increased with decreasing concentration of all dicarboxylic acids measured. Sequence of enthalpic stabilization on mixing was MBA mixing was MBA < DHBA < MPA < HMBA < HBS at 0.5 mass%.
  • T. Kimura; M. A. Khan; T. Kamiyama
    JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 85 3 575 - 580 2006年09月 
    Enthalpies of mixing of R- and S-enantiomers of liquid chiral compounds such as 2-aminohexane, 2-aminoheptane, 2-aminooctane, 2-aminononane, 1-( 4-chlorophenyl)-ethylamine, 1-(4-fluorophenyl) ethylamine, 2-amino-butane- 1-ol have been measured over the whole range of mole fractions at 298.15 K. Mixing of heterochiral liquids observed, realized enthalpic destabilization over entire compositions. The extreme values of enthalpies of mixing and the intermolecular interaction obtained by the molecular mechanics calculations showed a linear correlation, except the few compounds measured.
  • Takayoshi Kimura; Mohammed Abdullah Khan; Masakazu Ishii; Tadashi Kamiyama
    CHIRALITY 18 8 581 - 586 2006年08月 
    Enthalpy change on the mixing of R- and S-enantiomers of chiral liquid compounds such as dimethyl malate (1), methyl 3-hydroxylbutanoate (2), 2-butanol (3), ethyl 4-chloro-3-hydroxylbutanoate (4), 1,3,3-trimethylbicycle-[2.2.1]heptan-2-one (5), 3,7-dimethyl-6-octenal (6), and 8-bromo-2,6-dimethyl-2-octene (7) is measured over the entire range of mole fractions at 298.15 K, albeit very small values. The mixing of chiral liquids of R-1 + S-1, R-2 + S-2, R-3 + S-3, R-6 + S-6, and R-7 + S-7 produces enthalpic destabilization over the entire range of mole fractions, while that of R-4 + S-4 and R-5 + S-5 shows enthalpic stabilization over entire compositions. Enthalpy change on mixing at an equimolar concentration and the intermolecular interaction obtained by the molecular mechanics calculations show a linear correlation, except for a few compounds measured.
  • T. Kimura; Mohammad A. Khan; Masakazu Ishii; Kana Ueda; Takanori Matsushita; Tadashi Kamiyama; Masao Fujisawa
    JOURNAL OF CHEMICAL THERMODYNAMICS 38 8 1042 - 1048 2006年08月 
    Enthalpies of mixing of (R)- and (S)-enantomers of liquid chiral compounds such as benzyl-(1-phenyl-ethyl)-amine (1), 1-phenylethylamine (2), 1-phenyl-ethanol (3), butyric acid oxiranylmethyl ester (4), 4-methyl-[1,3]dioxolan-2-one (5), 2-Chloromethyloxirane (6) and 3-hydroxyisobutyric acid methyl ester (7) have been measured over the whole range of mole fractions at 298.15 K, albeit very small values. Mixing of heterochiral liquids of R-1 + S-1, R-5 + S-5, and R-7 + S-7 realized enthalpic stabilization over the whole range of mole fractions, whereas that of R-2 + S-2, R-3 + S-3, R-4 + S-4, and R-6 + S-6 realized enthalpic destabilization over entire compositions. The extreme values of enthalpies of mixing and the intermolecular interaction obtained by the molecular mechanics calculations showed a linear correlation, except few the compounds measured. (c) 2005 Elsevier Ltd. All rights reserved.
  • M Iwakura; K Maki; H Takahashi; T Takenawa; A Yokota; K Katayanagi; T Kamiyama; K Gekko
    JOURNAL OF BIOLOGICAL CHEMISTRY 281 19 13234 - 13246 2006年05月 
    We developed a strategy for finding out the adapted variants of enzymes, and we applied it to an enzyme, dihydrofolate reductase ( DHFR), in terms of its catalytic activity so that we successfully obtained several hyperactive cysteine- and methionine-free variants of DHFR in which all five methionyl and two cysteinyl residues were replaced by other amino acid residues. Among them, a variant ( M1A/M16N/M20L/M42Y/C85A/M92F/C152S), named as ANLYF, has an approximately seven times higher k(cat) value than wild type DHFR. Enzyme kinetics and crystal structures of the variant were investigated for elucidating the mechanism of the hyperactivity. Steady-state and transient binding kinetics of the variant indicated that the kinetic scheme of the catalytic cycle of ANLYF was essentially the same as that of wild type, showing that the hyperactivity was brought about by an increase of the dissociation rate constants of tetrahydrofolate from the enzyme-NADPH-tetrahydrofolate ternary complex. The crystal structure of the variant, solved and refined to an R factor of 0.205 at 1.9-A resolution, indicated that an increased structural flexibility of the variant and an increased size of the N-( p-aminobenzoyl)-L-glutamate binding cleft induced the increase of the dissociation constant. This was consistent with a large compressibility ( volume fluctuation) of the variant. A comparison of folding kinetics between wild type and the variant showed that the folding of these two enzymes was similar to each other, suggesting that the activity enhancement of the enzyme can be attained without drastic changes of the folding mechanism.
  • Thermodynamic properties of D- and L-tartaric acid in aqueous and ethanol solution at 298.15 K
    木村 隆良; Mohammed Abdullah Khan; 神山 匡; 藤澤 雅夫
    J. Chem. Eng. Data 51 4 909 - 913 American Chemical Society 2006年04月 
    298.15 Kにおける D- および L-酒石酸水溶液及びエタノール溶液の混合エンタルピーを決定し、その濃度変化ならびに安定性について溶媒効果をしらべた。また溶液中おける酒石酸の分子充填の様子を明らかにした。(英文)
  • Excess enthalpies of { Methyl Methylthiomethyl Sulfoxide + (CnH2n+1OH, n=4~12)} and {Dimethyl Sulfoxide +(CnH2n+1OH, n=4~12) } at 298.15 K
    木村 隆良; 松下隆宣; 水野浩也; 上林正尚; 藤澤 雅夫; 神山 匡; 高木定夫; 桃木町男; 利康義雄
    Thermochimica Acta 424 83 - 90 Elsevia 2004年11月 
    Excess enthalpies of nine mixtures between methyl methylthiomethyl sulfoxide (MMTSO) or dimethyl sulfoxide (DMSO) and each of aliphatic alcohols{CH3(CH2)nOH, n=3 to 11} have been determined at 298.15 K. All the systems show the positive enthalpies over the whole range of mole fraction. Excess enthalpies of MMTSO + alcohols are larger than that of DMSO + alcohols and increased with increasing the number of methylene groups of alcohols. The excess partial molar enthalpies at infinite dilution of butane-1-ol showed the boundary between the two linear relationships obtained between those of MMTSO or DMSO and dipole-dipole interaction of aliphatic alcohols{CH3(CH2)nOH, n=0 to 11}-sulfoxides.
  • 木村 隆良; 松下隆宣; 神山 匡; 高木定夫
    J. Chem & Eng data 49 4 1046 - 1051 American chemical Society 2004年07月 
    Excess enthalpies of binary mixtures between each of {CH3(CH2)nCN, n=5 to 12} and methyl methylthiomethyl sulfoxide or dimethyl sulfoxide have been determined at 298.15 K. All mixtures show positive enthalpy changes over the whole range of mole fractions. Excess enthalpies of the mixtures of nitriles increased with increasing size of the aliphatic groups. The increments of dipole interaction terms of ?12?22(r1+r2)-6 on excess partial molar enthalpies at infinite dilution showed different behavior on the border of pentanenitrile.
  • Takayoshi Kimura; Takanori Matsushita; Tadashi Kamiyama
    Journal of Solution Chemistry 33 6-7 875 - 886 2004年06月 
    The enthalpies of solution of aliphatic compounds [{aliphatic amine, H(CH 2) nNH 2, n = 3 to 10}, aliphatic benzene {H(CH 2) nC 6H 5, n = 0 to 8}, and alkane {H(CH 2) nH, n = 6 to 10}] in dimethyl sulfoxide have been measured at 298.15 K in the low concentration range from x = 5 × 10 -6 to x = 0.002. The partial molar enthalpies at infinite dilution of each aliphatic compound were determined and were found to increase linearly with increasing number of methylene groups. The enthalpic group contribution of methylene, phenyl, methyl, hydroxyl, nitrile, and amine in aliphatic compounds were 1.55, 2.65, 3.81, -2.55, -3.71, and -4.43 kJ-mol -1, respectively.
  • T Kimura; T Matsushita; T Kamiyama
    THERMOCHIMICA ACTA 416 1-2 129 - 134 2004年06月 
    Enthalpies of solution of aliphatic alcohols {CH3(CH2)(n)OH, n = 0-10} and aliphatic nitrites {CH3(CH2)(n)CN, n = 0-12} in dimethyl sulfoxide (DMSO) have been determined at 298.15 K for the mole fraction range 5 x 10(-5) to 0.002. In all cases enthalpies of solution were found to be positive. Partial molar enthalpies of solution at infinite dilution were derived. Linear relations between the limiting excess partial molar enthalpies and the number of methylene groups were found for both groups of compounds investigated. McMillan-Mayer's parameters, h(xx) and h(xxx), showed unfavorable and favorable interactions, respectively. (C) 2003 Elsevier B.V. All rights reserved.
  • T Kimura; T Matsushita; K Ueda; F Aktar; T Matsuda; T Kamiyama; M Fujisawa
    THERMOCHIMICA ACTA 414 2 209 - 214 2004年05月 [査読有り]
     
    Enthalpies of mixing of (R)- and (S)-enantiomers of liquid chiral compounds such as heptane-2-ol, octane-2-ol, nonane-2-ol, 3-chloropropane-1,2-diol and 2-methyl-1,4-butanediol have been measured over a range of mole fractions at 298.15 K, albeit very small values. Mixing of heterochiral liquids of heptane-2-ol, octane-2-ol, nonane-2-ol, 3-chloro-propane-1,2-diol, realized enthalpic destabilization over the whole range of mole fractions, whereas that of 2-methyl-1,4-butanediol realized enthalpic stabilization over entire compositions. The maximum values of enthalpies of mixing and the intermolecular interaction of cohesive energy density and entropy of vaporization showed a linear correlation except for the compounds having two chiral centers and others. (C) 2004 Elsevier B.V. All rights reserved.
  • Enthalpy changes on mixing two couples of S- and R-enantiomers of heptane-2-ol, octane-2-ol, nonane-2-ol, 3-chloro-propane-1,2-diol, 2-methyl-1,4-butanediol at 298.15 K
    木村 隆良; 松下隆宣; 植田佳奈; 松田哲雄; 神山 匡; 藤澤 雅夫; Farida AKTAR
    Thermochimica Acta 405 129 - 139 Elsevier 2004年05月 
    Enthalpies of mixing of (R)- and (S)-enantiomers of liquid chiral compounds such as heptane-2-ol, octane-2-ol, nonane-2-ol, 3-chloropropane-1,2-diol and 2-methyl-1,4-butanediol have been measured over a range of mole fractions at 298.15 K, albeit very small values. Mixingof heterochiral liquids of heptane-2-ol, octane-2-ol, nonane-2-ol, 3-chloro-propane-1,2-diol, realized enthalpic destabilization over the wholerange of mole fractions, whereas that of 2-methyl-1,4-butanediol realized enthalpic stabilization over entire compositions. The maximum values of enthalpies of mixing and the intermolecular interaction of cohesive energy density and entropy of vaporization showed a linearcorrelation except for the compounds having two chiral centers and others.
  • Takayoshi Kimura; Yuichi Kasai; Tomohide Tsujimoto; Emi Kawamura; Tadashi Kamiyama; Hatsumi Aki
    J. Mass. Spectrom. Soc. Jpn. 51 2 242 - 246 The Mass Spectrometry Society of Japan 2003年 [査読有り]
     
    Thermal behaviors of the inclusion compounds of deoxycholic acid with methanol, ethanol, propane-1-ol, propane-2-ol, and butane-1-ol have been studied by TG-DTA-MS and DSC. Liberation processes of guest molecules from their inclusion compounds of methanol, ethanol were carried out in a single step. Those of propanols and butane-1-ol from the corresponding inclusion compounds proceed in complicated steps. The elimination temperatures, enthalpies and the activation enthalpies of eliminations have been determined by TG, DSC, and MS.
  • K Gekko; T Kamiyama; E Ohmae; K Katayanagi
    TRENDS IN HIGH PRESSURE BIOSCIENCE AND BIOTECHNOLOGY, PROCEEDINGS 19 63 - 70 2002年 
    The partial specific volume (v(o)) and adiabatic compressibility (beta(s)(o)) of E. coli dihydrofolate reductase (DHFR) are largely influenced by binding ligands (DHF, THF, NADPH, NADP+, and methotrexate) and single amino acid substitutions at Gly67, Gly121, and Ala145 located in three flexible loops (18 mutants). The variations in v(o) and beta(s)(o) are mainly ascribed to the amount of cavity with a minor contribution of hydration (solvent accessible surface area). The beta(s)(o) of DHFR changes alternatively by binding or releasing the coenzyme and substrate. A mutant with a large beta(s)(o) value shows high enzymatic activity. These results demonstrate that a small alteration in the local structure due to ligand binding and mutation is dramatically magnified in the overall protein dynamics to affect the function, possibly via modification of cavity or long range interactions.

書籍

  • 化学便覧 基礎編 改訂6版
    (担当:分担執筆範囲:10.6.3 熱的性質・希釈エンタルピー・生体関連物質)日本化学会・丸善 2021年01月
  • 熱量測定・熱分析ハンドブック 第3版
    神山 匡 (担当:分担執筆範囲:タンパク質の安定性(DSC)-安定化剤-; 糖と低分子の結合(ITC))丸善出版 2020年08月
  • 日本の科学者
    神山 匡 (担当:分担執筆範囲:シクロデキストリンの包接におけるエントロピーの役割)本の泉社 2019年03月

講演・口頭発表等

  • タンパク質の構造・熱安定性に及ぼす添加物の影響  [招待講演]
    神山 匡
    熱・エントロピー科学シンポジウム(STES2019) 2019年06月 シンポジウム・ワークショップパネル(指名)
  • Cytochrome cおよびBSAの熱安定性に及ぼすシクロデキストリンの包接効果  [通常講演]
    神山 匡; 木村 隆良
    2012年09月
  • Effects of cyclodextrin on thermal stability of cytochrome c and Bovine serum albumin  [通常講演]
    神山 匡; 木村 隆良
    ICTAC15 2012年08月 ICTAC15
  • VOLUME FLUCTUATION OF BIOMOLECULES AS REVEALED BY ISOTHERMAL COMPRESSIBILITY AND THERMAL EXPANSION COEFFICIENT  [通常講演]
    神山 匡; 木村 隆良
    5th Internationa Symposium on Molecular Science of Fluctuations toward Biological Functions 2012年01月 Nara 5th Internationa Symposium on Molecular Science of Fluctuations toward Biological Functions
  • Stability and unfolding of 1H-3-Hydroxy-4-oxoquinaldine 2,4-Dioxygenase.  [通常講演]
    神山 匡; J. Guddorf; A. Albers; S. Fetzner; H.-J. Hinz
    日本生物物理学会 2007年12月 横浜 日本生物物理学会
  • Stability and unfolding of 1H-3-Hydroxy-4-oxoquinaldine 2,4-Dioxygenase.  [通常講演]
    神山 匡; J. Guddorf; A. Albers; S. Fetzner; H.-J. Hinz
    日本熱測定学会 2007年10月 札幌 日本熱測定学会
  • リゾチームの熱安定性に及ぼす修飾化シクロデキストリンの添加効果  [通常講演]
    神山 匡; 木村 隆良
    日本熱測定学会 2007年10月 札幌 日本熱測定学会
  • The degradation process of protein in aqueous solution by microcalorimeter  [通常講演]
    神山 匡; 戸島 由実
    日本熱測定学会 第41回シンポジウム 2006年03月 近畿大学(大阪) 日本熱測定学会 第41回シンポジウム
     
    In order to research the protein and preserve the food, it is very important to observe and prevent putrefaction (degradation) of these substances. In this study, the degradation process of proteins in aqueous solutions were measured by microcalorimeter, UV, CD, and viscosimetry at 298.15 K and thermodynamic and kinetic analyses of the degradation were performed.
  • アミノ酸との相互作用を指標にしたシクロデキストリ ンによるタンパク質の不安定化機構  [通常講演]
    神山 匡; 山根 拓也; 北宿 智嗣; 木村 隆良
    日本化学会第86春季年会 2006年03月 日本大学(千葉県) 日本化学会第86春季年会
     
    和文概要:タンパク質水溶液にシクロデキストリンを添加すると タンパク質の安定性が低下する。この不安定化機構を明らかにす るために、アミノ酸とシクロデキストリンの相互作用を体積を指 標にして明らかにした。 英文概要:The stability of protein was decreased by adding of cyclodextrin to the solution. Cyclodextrin destablized protein in aqueous solution.To clarify the mechanism of the destabilization, the interaction of the amino acid and the cyclodextrin was clarified studied as revealed by apparent partial specific volume.
  • Volume changes of Cyclodextrin with amino acids in aqueous solution  [通常講演]
    神山 匡; 山根 拓也; 木村 隆良
    PACIFICHEM2005 2005年12月 Hawaii PACIFICHEM2005
     
    It is important to clarify the molecular identification mechanism and the intermolecular interaction in vivo. The densities of various aqueous alpha- and methyl beta- Cyclodextrin (CD) solutions with 19 kinds of amino acids were respectively measured at 298.15 K. Influences on the molecular recognition by difference in properties of side chain of amino acids were discussed by the obtained molar volume changes of inclusion compounds and the binding constants.
  • TG-DTAを用いた蛋白質の水和・脱水和  [通常講演]
    神山 匡; 戸島 由実; 木村 隆良
    日本熱測定学会 第41回熱測定討論会 2005年10月 九州大学(福岡) 日本熱測定学会 第41回熱測定討論会
     
    種々のタンパク質の粉末結晶についてTG-DTA測定を行い、粉末結晶中におけるタンパク質水和量の決定と脱水にともなう活性化エネルギーを決定した。これらの結果をアミノ酸組成や分子量と比較すると共に、既存のX線結晶構造データと比較した。
  • Thermodynamic and kinetic study of enzymatic reaction by microcalorimeter  [通常講演]
    戸島 由実; 神山 匡; 木村 隆良
    CATS-2005 2005年10月 Fukuoka(JAPAN) CATS-2005
  • Thermodynamic study of metabolic reaction in various aqueous protein solutions  [通常講演]
    神山 匡; 戸島 由実; 木村 隆良
    CATS-2005 2005年10月 Fukuoka(JAPAN) CATS-2005
     
    In researching the preservation of protein and food, it is very important to observe and prevent putrefaction of the substances. Calorimeters with high stability and sensitivity are suitable for measuring slow thermal reactions such as enzyme activity or solid reactions, because the calorimeter can detect total thermal reactions of samples both thermodynamically and kinetically [1,2]. We studied the metabolic reactions in aqueous myoglobin solutions at various conditions by calorimeter and thermodynamic and kinetic analyses of metabolism were also performed [3]. In this study, the metabolic reactions in various aqueous protein solutions were measured by microcalorimeter at 298.15 K and thermodynamic and kinetic analyses of metabolism were performed.
  • Thermal expansion coefficients of inclusion compounds of alpha-, beta-, and gamma-cyclodextrin in aqueous solutions  [通常講演]
    石井 亮太; 神山 匡; 木村 隆良
    CATS-2005 2005年10月 Fukuoka (JAPAN) CATS-2005
  • デオキシコール酸包接化合物のTG―MS  [通常講演]
    木村 隆良; 足立祐樹; 中西雄大; 神山 匡
    第52回質量分析総合討論会 2005年06月 名古屋市 第52回質量分析総合討論会
  • 高活性DHFR変異体の圧縮率による揺らぎの同定  [通常講演]
    月向 邦彦; 神山 匡; 片柳 克夫; 巌倉 正寛
    日本生物物理学会 2004年11月 日本生物物理学会
  • DMSO水溶液中におけるリゾチームの変性機構  [通常講演]
    神山 匡
    日本溶液化学会 第27回溶液化学シンポジウム プレシンポジウム 2004年11月 東京電機大学(埼玉) 日本溶液化学会 第27回溶液化学シンポジウム プレシンポジウム
     
    タンパク質の立体構造形成や安定性において溶媒は重要な役割を担っている。本研究では水と任意の比率で混合し、水と強い相互作用を示す非電離性両親媒性溶媒のジメチルスルフォキシド(DMSO)を用い、さまざまなDMSOモル分率中におけるリゾチームの構造変化を蛍光スペクトル、密度、粘度測定を用いて298.15 Kにおいて観測し、DMSO変性にともなうリゾチームの部分比容、固有粘度、粘性流動に関する活性化自由エネルギーを決定すると共に、選択的溶媒和量変化を用いてリゾチームのDMSO変性機構を考察した。
  • 水溶液中でのシクロデキストリン空洞へのニトリルの包接に伴うエンタルピーおよびエントロピー変化  [通常講演]
    木村 隆良; 雪山 聖; 神山 匡; 藤澤 雅夫
    第22回シクロデキストリンシンポジウム 2004年09月 熊本市 第22回シクロデキストリンシンポジウム
  • ENTHALPIES OF SOLUTION OF INCLUSION COMPOUNDS OF DEOXYCHOLIC ACID  [通常講演]
    野口耕一; 木村 隆良; 神山 匡
    18th IUPAC International Conference on Chemical Thermodynamics 2004年08月 Beijing, China 18th IUPAC International Conference on Chemical Thermodynamics
  • Enthalpies of mixing of d- and l- tartaric acid in aqueous and ethanol solution  [通常講演]
    木村 隆良; Abdulla Khan; 神山 匡
    18th IUPAC International Conference on Chemical Thermodynamics 2004年08月 Beijing, China 18th IUPAC International Conference on Chemical Thermodynamics
  • THERMODYNAMIC STUDY ON THE SLOW REACTION OF PROTEIN IN AQUEOUS SOLUTION  [通常講演]
    神山 匡; 戸島 由実; 松下 隆宣; 木村 隆良
    IUPAC ICCT-2004 2004年08月 Peking IUPAC ICCT-2004
     
    The high sensitive and stable calorimeter is suitable for measuring slow thermal reactions like an enzyme activity or a solid reaction. Such slow reactions can sensitively reflect so many complicated molecular interactions and conformational changes although it may be difficult to quantitatively divide the differences of the interactions. In this work, the slow reactions which were occurred in myoglobin aqueous solutions were measured at 298.15 K and thermodynamic and kinetic study about the slow reactions were carried out. Aqueous solutions of myoglobin were measured by a twin micro-calorimeter of heat conduction type (Thermal Activity Monitor) with a sealed glass cell for solution. Myoglobin solutions were prepared by dissolving with water and/or hydrochloric acid. The slow reactions were measured under various conditions such as pH and volume of solution (V), concentration of myoglobin (c), and incubation temperature.
  • EXCESS ENTHALPIES OF BINARY MIXTURES OF AMINES + PROPANOLS AT 298.15 K  [通常講演]
    木村 隆良; 北井良子; 神山 匡
    18th IUPAC International Conference on Chemical Thermodynamics 2004年08月 Beijing, China 18th IUPAC International Conference on Chemical Thermodynamics
  • EXCESS ENTHALPIES OF ALIPHATIC ACETATE + METHYL METHYLTHIOMETHYL SULFOXIDE, + DIMETHYL SULFOXIDE  [通常講演]
    松下隆宣; 木村 隆良; 神山 匡
    18th IUPAC International Conference on Chemical Thermodynamics 2004年08月 Beijing, China 18th IUPAC International Conference on Chemical Thermodynamics
  • THERMODYNAMIC STUDIES ON THE SYSTEM OF METHANOL + ETHANOL AND + PROPANE-1-OL  [通常講演]
    乾 真也; 木村 隆良; 神山 匡
    18th IUPAC International Conference on Chemical Thermodynamics 2004年08月 Beijing, China 18th IUPAC International Conference on Chemical Thermodynamics
  • 水溶液中でのα‐シクロデキストリン+ブタンジオール包接化合物の熱力学的性質  [通常講演]
    木村 隆良; 神山 匡; 藤澤 雅夫
    日本化学会第84春季年会 2004年03月 関西学院大学 西宮市 日本化学会第84春季年会
  • 脂肪族アセテート+FAMSO,+DMSO 系の混合熱  [通常講演]
    松下隆宣; 木村 隆良; 神山 匡
    日本化学会第84春季年会 2004年03月 関西学院大学 西宮市 日本化学会第84春季年会
  • デオキシコール酸-ブタノール系包接化合物の溶解熱  [通常講演]
    野口 耕一; 木村 隆良; 神山 匡
    日本化学会第84春季年会 2004年03月 関西学院大学 西宮市 日本化学会第84春季年会
  • アミン+アルコールの三成分溶液下における過剰エンタルピー  [通常講演]
    林 英貴; 木村 隆良; 神山 匡
    日本化学会第96春季年会(京都)
  • a-ラクトアルブミンの構造変化と柔軟性の相関  [通常講演]
    石田 将忠; 丸谷 智迦津; 神山 匡
    日本化学会第96春季年会(京都)
  • 1-alkyl-3-methylimidazorium iodide水溶液の過剰熱力学量  [通常講演]
    吉田 祥; 木村 隆良; 神山 匡
    日本化学会第96春季年会(京都)
  • タンパク質の圧縮率に与えるイオン液体の効果  [通常講演]
    下谷 一貴; 木村 隆良; 神山 匡
    日本化学会第96春季年会(京都)
  • Thermodynamic properties of aggregate state of proteins in dioxane aqueous Solutions  [通常講演]
    Tadashi KAMIYAMA; Takashi INOMATA; Takayoshi KIMURA
    Pacifichem2015(Hawaii)
  • Excess enthalpies of propylamine and alcohol isomers  [通常講演]
    Hidetaka Hayashi; Tadashi Kamiyama; Takayoshi Kimura
    Pacifichem2015(Hawaii)
  • Volume fluctuation of protein in ionic liquid aqueous solutions at various temperatures  [通常講演]
    Kazuki Shimotani; Takashi Inomata; Takayoshi Kimura; Tadashi Kamiyama
    Pacifichem2015(Hawaii)
  • Stability of protein in ionic liquids at various mole fraction  [通常講演]
    Madoka Okabe; Toshiki Miki; Takayoshi Kimura; Tadashi Kamiyama
    Pacifichem2015(Hawaii)
  • 水中におけるイオン液体+シクロデキストリン+タンパク質間相互作用  [通常講演]
    林 英貴; 三木 稔生; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • プロピルアミン+アルコール異性体の過剰エンタルピー  [通常講演]
    林 英貴; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • ジオキサン水溶液中におけるタンパク質の凝集体形成機構  [通常講演]
    丸谷 智迦津; 福本 彩華; 猪股 孝史; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • a-Lactalbuminのソルビトール誘起モルテングロビュール状態の熱力学的研究  [通常講演]
    丸谷 智迦津; 石田 将忠; 森 麻由香; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • 1-Alkyl-3-methylimidazolium iodide及びCyclodextrin水溶液中でのタンパク質の熱安定性  [通常講演]
    岡部 円香; 三木 稔生; 田口 真依帆; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • 高圧下における三成分溶液の等温圧縮率  [通常講演]
    下谷 一貴; 木村 隆良; 神山 匡
    第51回熱測定討論会(埼玉)
  • 溶解熱測定によるタンパク質の構造転移熱測定  [通常講演]
    花岡 洸樹; 神山 匡; 木村 隆良
    第51回熱測定討論会(埼玉)
  • イオン液体+水系の過剰熱力学量  [通常講演]
    吉田 祥; 神山 匡; 木村 隆良
    第51回熱測定討論会(埼玉)
  • 第2族金属塩水和物結晶の脱水機構  [通常講演]
    吉田 祥; 宮地 敏浩; 神山 匡; 木村 隆良
    第51回熱測定討論会(埼玉)
  • 低モル分率イオン液体水溶液中におけるタンパク質の体積−圧力相関  [通常講演]
    下谷 一貴; 神山 匡
    第15回日本蛋白質科学会(徳島)
  • ミオグロビンのソルビトール誘起モルテングロビュール  [通常講演]
    丸谷 智迦津; 加藤 大; 浜田 拓也; 木村 隆良; 神山 匡
    第15回日本蛋白質科学会(徳島)
  • FAMSO+プロパノール系の蒸気圧  [通常講演]
    木村隆良; 高橋雅; 西沢諒太; 神山匡; 藤沢雅夫
    第50回熱測定討論会(大阪)
  • 胆汁酸-水-包接化合物の熱分析  [通常講演]
    木村隆良; 岡部円香; 山口慎太郎; 神山匡
    第50回熱測定討論会(大阪)
  • Aspergillusoryzaeの熱測定  [通常講演]
    木村隆良; 小林直矢; 水田直樹; 神山匡; 藤澤雅夫
    第50回熱測定討論会(大阪)
  • ペンタノール+プロピルアミン系の混合エンタルピー  [通常講演]
    木村隆良; 中山庄太郎; 林英貴; 神山匡; 藤澤雅夫
    第50回熱測定討論会(大阪)
  • 第2族塩化物水和結晶の脱水過程  [通常講演]
    木村隆良; 宮地敏浩; 吉田祥; 神山匡
    第50回熱測定討論会(大阪)
  • Methyl-b-Cyclodextrin水溶液中におけるアミノ酸の溶解度  [通常講演]
    濱千拡; 北株慎士; 神山匡; 木村隆良
    第50回熱測定討論会(大阪)
  • 生体高分子の構造および安定性に及ぼす糖添加効果  [通常講演]
    加藤圭一; 五百旗頭孝之; 加藤大; 神山匡; 木村隆良
    第50回熱測定討論会(大阪)
  • 等温圧縮率を指標としたイオン液体水溶液中におけるタンパク質の体積揺らぎ  [通常講演]
    下谷一貴; 神山匡; 木村隆良
    第50回熱測定討論会(大阪)
  • 1-Hexyl-3-methylimidazoliumiodide+水+タンパク質の断熱圧縮率および熱膨張率  [通常講演]
    金川奈央; 神山匡; 木村隆良
    第50回熱測定討論会(大阪)
  • リモネン+アルコール系の混合エンタルピー  [通常講演]
    木村隆良; 小菅友理; 神山匡; 藤澤雅夫
    第50回熱測定討論会(大阪)
  • 溶液中におけるキラルフェンコンのエンタルピー識別  [通常講演]
    冨樫平; 神山匡; 藤澤雅夫; 木村隆良
    第50回熱測定討論会(大阪)
  • シクロデキストリン及びイオン液体水溶液中におけるタンパク質の熱安定性  [通常講演]
    三木稔生; 神山匡; 木村隆良
    第50回熱測定討論会(大阪)
  • ミオグロビンのソルビトール誘起モルテングロビュール状態の熱力学的研究  [通常講演]
    丸谷智迦津; 神山匡; 加藤大; 浜田拓也; 木村隆良
    第50回熱測定討論会(大阪)
  • Enthalpic descrimination of chiral fenchones in various solutions  [通常講演]
    H. Togashi; T. Kamiyama; M. Fujisawa; T. Kimura
    The 7th international and the 9th China-Japan Symposium on Calorimetry and Thermal Analysis(China)
  • Thermal stability of proteins in Cyclodextrin and Ionic liquid aqueous solutions  [通常講演]
    Toshiki MIKI; Tadashi KAMIYAMA; Takayoshi KIMURA
    The 7th international and the 9th China-Japan Symposium on Calorimetry and Thermal Analysis(China)
  • Thermodynamic properties of sorbitol-induced molten globule state of Myoglobin  [通常講演]
    Tomokadu MARUTANI; Tadashi KAMIYAMA; Dai KATO; Takuya HAMADA; Takayoshi KIMURA
    The 7th international and the 9th China-Japan Symposium on Calorimetry and Thermal Analysis(China)
  • Dehydration of alkali-earth metal chloride hydrates  [通常講演]
    T. Kimura; T. Miyaji; S. Yoshida; T. Kamiyama
    The 7th international and the 9th China-Japan Symposium on Calorimetry and Thermal Analysis(China)
  • Mechanism of Denaturation and Aggregate Formation of Proteins in Dioxane Aqueous Solutions  [通常講演]
    Tadashi KAMIYAMA; Takashi INOMATA; Takayoshi KIMURA
    International Conference of Chemical Thermodynamics 2014(南アフリカ)
  • ソルビトール誘起モルテングロビュール状態の熱力学的研究/  [通常講演]
    丸谷 智迦津; 加藤大; 浜田 拓也; 木村隆良; 神山匡
    第14回日本蛋白質科学会年会(横浜)
  • Cyclodextrin及び1-Alkyl-3-methylimidazolium iodide水溶液中におけるタンパク質の熱安定  [通常講演]
    三木 稔生; 木村隆良; 神山匡
    第14回日本蛋白質科学会年会(横浜)
  • FAMSO + 脂肪族アルコール系における熱力学的研究  [通常講演]
    高橋雅; 山口淳; 神山匡; 藤澤 雅夫; 脇坂昭弘; 木村隆良
    第49回熱測定討論会(千葉)
  • 構造異性体中における光学活性リモネンのキラル認識  [通常講演]
    小菅 友理; 神山 匡; 藤澤 雅夫; 木村 隆良
    第49回熱測定討論会(千葉)
  • 水溶液中でのα-シクロデキストリン+ブタン化合物の包接化機構  [通常講演]
    辻川 哲史; 藤江 智之; 神山 匡; 藤澤 雅夫; 木村 隆良
    第49回熱測定討論会(千葉)
  • ジオキサン水溶液中におけるタンパク質の変性と凝集体形成機構  [通常講演]
    猪股 孝史; 神山 匡; 木村 隆良
    第49回熱測定討論会(千葉)
  • ソルビトール誘起モルテングロビュール状態の熱力学的及び速度論的性質  [通常講演]
    神山 匡; 加藤 大; 木村 隆良
    第49回熱測定討論会(千葉)
  • 様々な溶媒中におけるキラルフェンコンの相互作用について  [通常講演]
    冨樫 平; 神山 匡; 藤澤 雅夫; 木村 隆良
    第49回熱測定討論会(千葉)
  • ブチルアミン+ブタノール系の混合エンタルピー  [通常講演]
    木村 隆良; 鈴木 辰規; 神山 匡; 藤澤 雅夫
    第49回熱測定討論会(千葉)
  • タンパク質の熱変性における部分比容、断熱圧縮率、熱膨張率  [通常講演]
    高岡徹郎; 浜田拓也; 木村隆良; 神山匡
    第51回日本生物物理学会(京都)
  • シクロデキストリン+タンパク質+メチルオレンジ・ヨウ素系における包接機構  [通常講演]
    丸谷智迦津; 木村隆良; 神山匡
    第51回日本生物物理学会(京都)
  • タンパク質の熱安定性に及ぼすシクロデキストリンの包接効果  [通常講演]
    三木稔生; 五百旗頭孝之; 木村隆良; 神山 匡
    第51回日本生物物理学会(京都)
  • THERMODYNAMIC PROPERTIES AND CONFORMATION OF PROTEIN IN WATER WITH ADDITIVES  [通常講演]
    Tadashi Kamiyama
    The 5th International Symposium on the New Frontiers of Thermal Studies of Materials(Yokohama)
  • Effects of Cyclodextrin on Stability of Proteins  [通常講演]
    Tadashi Kamiyama
    3rd European Conference on Cyclodextrins (Turky)
  • シクロデキストリン+メチルオレンジ+ヨウ素系における包接機構  [通常講演]
    丸谷智迦津; 神山匡; 木村隆良
    第30回シクロデキストリンシンポジウム(熊本)
  • 球状タンパク質の熱安定性に及ぼすMe-β-CDの包接効果  [通常講演]
    三木稔生; 神山匡; 木村隆良
    第30回シクロデキストリンシンポジウム(熊本)

MISC

受賞

  • 日本熱測定学会奨励賞(2011)

共同研究・競争的資金等の研究課題

  • 日本学術振興会:科学研究費助成事業
    研究期間 : 2009年 -2010年 
    代表者 : 神山 匡
     
    蛋白質の揺らぎは機能を発現する上で重要な要因であり、その定量化が望まれている。本研究では蛋白質の構造的な体積揺らぎを反映する等温圧縮率を決定するために、高温・高圧での密度測定(10^<-6>g/cm^3)が可能な装置の開発と改良および測定を行った。 【装置改良】新たな試料導入口の設置と圧媒体に水を使用することにより、測定試料量を25mlから4mlへの減量化に成功した。この減量化により、希少サンプルの測定が可能となった。【等温圧縮率測定】改良した高温・高圧密度計を用いて球状蛋白質(Bovine Albumin、Chicken Albumin、Lysozyme)、アミノ酸(Proline、Glutamic acid)、糖(Cyclodextrin)の水溶液における高温・高圧密度測定を行い、各温度における等温圧縮率を決定した。Prolineの等温圧縮率は負であったが、他の溶質は正の圧縮率を示しており、立体構造の形成に伴う内部の空間(cavity)が圧縮スペースになっていることが分かった。タンパク質の等温圧縮率は断熱圧縮率(文献値)よりも大きい傾向があり、圧縮率から求まる25℃における体積揺らぎは、全体積のおよそ0.22-0.38%に相当することがわかった。等温圧縮率は温度と共に増加し、また、体積揺らぎの割合は分子量が大きいほど小さくなっており、熱振動に由来する揺らぎとcavityに由来する揺らぎが存在することが示唆された。【熱膨張率測定】高度分岐環状デキストリンの熱膨張率は球状蛋白質の数倍であったことから、蛋白質は糖鎖よりも分子内相互作用が強く、熱的振動の影響を受けにくいことが分かった。
  • タンパク質の機能・構造相関に関する熱力学的研究
    共同研究
  • Thermodynamic studies on the interaction between conformation and function of protein
    Cooperative Research

委員歴

  • 2020年10月 - 現在   日本熱測定学会   副編集委員長
  • 2019年10月 - 2021年09月   日本熱測定学会   庶務幹事

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