KINDAI UNIVERSITY


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鷲尾 洋平ワシオ ヨウヘイ

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所属部署名水産研究所
職名助教
学位博士(農学)
専門魚類発生工学
ジャンル経営・産業/漁業
コメンテータガイドhttp://www.kindai.ac.jp/meikan/1433-washio-youhei.html
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Last Updated :2017/09/15

コミュニケーション情報 byコメンテータガイド

コメント

    主に、海産の増養殖対象種であるマダイ(生殖細胞の雌雄分化)およびヒラメ(変態期の左右性形成)に関して、発生工学的・遺伝学的見地から研究を行っています。

研究活動情報

論文

  • Ocular-side lateralization of adult-type chromatophore precursors: development of pigment asymmetry in metamorphosing flounder larvae., Washio Y, Aritaki M, Fujinami Y, Shimizu D, Yokoi H, Suzuki T, Journal of experimental zoology. Part B, Molecular and developmental evolution, 320, 3, 151, 165,   2013年05月, 査読有り
  • Continuous illumination through larval development suppresses dopamine synthesis in the suprachiasmatic nucleus, causing activation of α-MSH synthesis in the pituitary and abnormal metamorphic skin pigmentation in flounder., Itoh K, Washio Y, Fujinami Y, Shimizu D, Uji S, Yokoi H, Suzuki T, General and comparative endocrinology, 176, 2, 215, 221,   2012年04月, 査読有り
  • Metamorphic pitx2 expression in the left habenula correlated with lateralization of eye-sidedness in flounder., Suzuki T, Washio Y, Aritaki M, Fujinami Y, Shimizu D, Uji S, Hashimoto H, Development, growth & differentiation, 51, 9, 797, 808,   2009年12月, 査読有り
  • Adult-type pigment cells, which color the ocular sides of flounders at metamorphosis, localize as precursor cells at the proximal parts of the dorsal and anal fins in early larvae., Watanabe K, Washio Y, Fujinami Y, Aritaki M, Uji S, Suzuki T, Development, growth & differentiation, 50, 9, 731, 741,   2008年12月, 査読有り
  • Flounder and fugu have a single lefty gene that covers the functions of lefty1 and lefty2 of zebrafish during L-R patterning., Hashimoto H, Uji S, Kurokawa T, Washio Y, Suzuki T, Gene, 387, 1-2, 126, 132,   2007年01月, 査読有り

MISC

  • Exposure to oxidative by-products during metamorphosis affects pigmentation patterns in flounder, Paralichthys olivaceus, Youhei Washio, Yuichiro Fujinami, Daisuke Shimizu, Hayato Yokoi, Tohru Suzuki, Aquaculture, 435, 318, 327,   2015年01月01日, 10.1016/j.aquaculture.2014.10.015, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84908336966&origin=inward
    概要:© 2014 Elsevier B.V. Hatcheries often employ disinfection by electrolysis for water; however, this produces free oxidants that pose practical problems in the production of flounder, Paralichthys olivaceus. Rearing juvenile flounder in electrolyzed seawater (EL-SW) causes pseudo-albinism. To elucidate the mechanism of the onset of EL-SW-induced pseudo-albinism, we compared thyroid hormone (TH) metabolism, melanoblasts, and skin differentiation in flounder reared in normal seawater (normal SW) and EL-SW and determined the critical period for the onset of pseudo-albinism. The number of thyroxin (T4)-positive thyroid follicles increased toward the metamorphic climax. The expression of deiodinase 1 (. dio1), which converts plasma T4 to triiodothyronine (T3), in the liver peaked synchronously with the increase in T4-positive thyroid follicles. EL-SW exposure had no effect on TH metabolism. Instead, EL-SW inhibited differentiation of adult-type melanoblasts in the skin of the ocular side together with a loss of structural asymmetry in the bilateral skins. Although EL-SW does not affect TH metabolism, our data indicate that it does inhibit the signaling system that controls differentiation of the blind and ocular lateral halves during the establishment of metamorphic asymmetry. The early-metamorphic stage, E- and F-stages, was demonstrated to be the critical period for the onset of pseudo-albinism in EL-SW-reared larvae. These results suggest that long-term exposure to free oxidants, even at low levels, acts as a chronic stressor on flatfish metamorphosis.
  • Staging of initial pepsinogen and chitinase expression and complete gastric gland development within the larval stomach of Japanese flounder, spotted halibut, seven-band grouper and greater amberjack, Xiaoming Wu, Youhei Washio, Masato Aritaki, Yuichiro Fujinami, Daisuke Shimizu, Hiroshi Hashimoto, Takashi Iwasaki, Susumu Uji, Tohru Suzuki, Aquaculture, 314, 165, 172,   2011年04月04日, 10.1016/j.aquaculture.2011.02.002, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79952738673&origin=inward
    概要:The gastric glands of marine aquaculture fish species are known to start secretion of pepsinogens (Pep) during larval development, far after first feeding. Although characterization of gastric gland development is considered useful for managing larval feeding, the timing of initial Pep synthesis by the gastric gland has only been reported for a few species. Consequently, this study employed in situ hybridization (ISH) to characterize the onset of expression of genes encoding the gastric enzymes Pep and Chitinase (Chi), as well as the complete development of gastric glands in the larval stomach of Japanese flounder (Paralichthys olivaceus), spotted halibut (Verasper variegatus), seven-band grouper (Epinephelus septemfasciatus) and greater amberjack (Seriola dumerili). The expression of both Chi and Pep was observed to begin in the cardiac region of the larval stomach at 25. days post-hatching (dph) in Japanese flounder, 36. dph in spotted halibut, 38. dph in seven-band grouper and 13. dph in greater amberjack. The gastric glands of these species were fully developed in the stomach approximately 5-12. days after the initiation of Pep expression. Development of gastric glands was observed to coincide with external manifestations of metamorphosis, such as the development of adult coloration in Japanese flounder and spotted halibut of the Pleuronectiformes, while it finished prior to adult-type coloration in seven-band grouper and greater amberjack of the Perciformes. © 2011 Elsevier B.V.