KINDAI UNIVERSITY


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財満 信宏ザイマ ノブヒロ

プロフィール

所属部署名農学部 応用生命化学科 / 農学研究科
職名准教授
学位博士(農学)
専門質量分析イメージング
ジャンル医療・健康/薬と社会
コメンテータガイドhttp://www.kindai.ac.jp/meikan/811-zaima-nobuhiro.html
ホームページURLhttp://kaken.nii.ac.jp/d/r/40455572.ja.html
メールアドレス
Last Updated :2017/11/18

コミュニケーション情報 byコメンテータガイド

コメント

    質量分析イメージングの開発・応用研究を行い、農作物中の機能性成分の分布や、摂取した機能性成分の体内動態を可視化するほか、疾患解析などにも応用しています。

学歴・経歴

学歴

  • 京都大学, 大学院農学研究科
  • 京都大学, 農学部

経歴

  •   2015年04月,  - 現在, 近畿大学(准教授)
  •   2011年04月,  - 2015年03月, 近畿大学(講師)
  •   2008年10月,  - 2011年03月, 浜松医科大学(特任助教)
  •   2007年04月,  - 2008年09月, 三菱化学生命科学研究所(特別研究員)

研究活動情報

研究分野

  • 境界医学, 病態検査学
  • 農芸化学, 食品科学

研究キーワード

  • 脂質代謝異常, 質量分析イメージング, 質量顕微鏡, 脂質蓄積ミオパチー, 脂質, ATGL変異症, 脂肪肝, 動脈硬化, 病理解析, 粥状動脈硬化, 腹部大動脈瘤, 自閉症

論文

  • 「成人食物アレルギーUpdate」に寄せる 新規大豆アレルゲンGly m7の検出と特性解析, 徳舛直樹, 花房佳世, 野地和音, 矢野えりか, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 503, 856‐859,   2017年08月31日
  • Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice., Kugo H, Zaima N, Onozato M, Miyamoto C, Hashimoto K, Yanagimoto K, Moriyama T, Food & function, 8, 8, 2829, 2835,   2017年08月, 査読有り
  • Antiosteoporotic activity of a syringic acid diet in ovariectomized mice., Tanaka T, Kawaguchi N, Zaima N, Moriyama T, Fukuta Y, Shirasaka N, Journal of natural medicines,   2017年07月, 査読有り
  • Pathological Analysis of the Ruptured Vascular Wall of Hypoperfusion-induced Abdominal Aortic Aneurysm Animal Model., Kugo H, Zaima N, Tanaka H, Hashimoto K, Miyamoto C, Sawaragi A, Urano T, Unno N, Moriyama T, Journal of oleo science, 66, 5, 499, 506,   2017年05月, 査読有り
  • The Effect of n-3 PUFA on the Development of Abdominal Aortic Aneurysm., Zaima N, Journal of atherosclerosis and thrombosis,   2017年05月, 査読有り
  • 「自然免疫とアレルギー疾患―最新の病態―」に寄せる 野菜・果実飲料のOASリスク評価, 村上浩規, 植田啄也, 矢野えりか, 岩本和子, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 37, 497, 265‐268, 268,   2017年03月20日
    概要:花粉症に関連するクラス2食物アレルギーにおいては、主な症例として豆乳や野菜・果物などの摂取時のOASが一般的である。この原因抗原として、汎アレルゲンであるPR-10やプロフィリンがよく知られている。そこで、本稿では、プロフィリン(Profilin)に注目し、各種野菜・果実飲料の可溶性画分における本アレルゲンを検出することで、プロフィリンによるOAS発症リスクを評価した。市販のこれら飲料では、加熱等による殺菌過程で、本アレルゲンは変性・不溶化しており、OASリスクは低いことが示唆された。(著者抄録)
  • The effects of nicotine administration on the pathophysiology of rat aortic wall, H. Kugo, N. Zaima, H. Tanaka, T. Urano, N. Unno, T. Moriyama, Biotechnic and Histochemistry, 92, 141, 148,   2017年02月17日
    概要:© 2017 The Biological Stain Commission. Abdominal aortic aneurysm (AAA) is the progressive dilation of the abdominal aorta. Nicotine is reported to be associated with the development and rupture of AAA, but the pathological effects of nicotine on normal rat aorta have not been determined. We investigated pathological changes in the aortic wall of rats caused by the administration of nicotine. Nicotine administration weakened the vascular wall, increased gelatinolytic activity and promoted the destruction of elastin and collagen in the rat abdominal aorta. There were no differences in the areas positive for matrix metalloproteinase (MMP)-2 and MMP-9 between the control and nicotine treated groups. The areas positive for MMP-12 in the nicotine group were significantly greater than for the control group. Gelatinolytic activity in the aortic wall was increased significantly in the nicotine group. Our findings suggest that MMP-12 is sensitive to nicotine exposure in rats.
  • 「蕁麻疹の原因と最新治療」に寄せる 各種大豆加工食品における花粉症関連大豆アレルゲンGly m3(プロフィリン)の検出, 植田啄也, 花房佳世, 矢野えりか, 岩本和子, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 37, 495, 58‐61, 61,   2017年01月20日
    概要:花粉症に関連する大豆アレルギー(大豆によるクラス2食物アレルギー)の主な症例としては、豆乳などの摂取時のOASが一般的である。大豆には、様々な加工食品が存在するが、それらのOASリスクは多様である。クラス2大豆アレルギーの主要抗原としてGly m 4とGly m 3が同定されている。そこで、各種大豆加工食品の抽出後の上清画分から可溶性のGly m 3を検出することで、Gly m 3によるOAS発症リスクを評価した。得られた結果はGly m 4の場合と類似していた。これら2つの花粉症関連抗原の上清中での検出量を評価することで、各種大豆加工食品のOASリスクの指標となることが示唆された。(著者抄録)
  • Hyperglycemia Suppresses RANKL-Induced Osteoclast Differentiation through LXRβ Expression in RAW264.7 Cells., Tanaka T, Takei Y, Zaima N, Moriyama T, Yamanouchi D, Journal of nutritional science and vitaminology, 63, 1, 28, 34,   2017年, 査読有り
  • Lysophosphatidylcholine Acyltransferase-3 Expression Is Associated with Atherosclerosis Progression., Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Yata T, Iwaki T, Umemura K, Sano H, Suzuki Y, Urano T, Setou M, Unno N, Journal of vascular research, 54, 4, 200, 208,   2017年, 査読有り
  • Significant advancement of mass spectrometry imaging for food chemistry, Yukihiro Yoshimura, Naoko Goto-Inoue, Tatsuya Moriyama, Nobuhiro Zaima, Food Chemistry, 210, 200, 211,   2016年11月01日
    概要:© 2016 Elsevier Ltd. All rights reserved. Food contains various compounds that have an impact on our daily lives. Many technologies have been established to analyze these molecules of interest in foods. However, the analysis of the spatial distribution of these compounds in foods using conventional technology, such as high-performance liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry is difficult. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is considered an ideal complementary approach. MALDI-MSI is a two-dimensional MALDI-MS technology that can detect compounds in a tissue section without extraction, purification, separation, or labeling. MALDI-MSI can be used to visualize the spatial distribution of chemical compounds or biomolecules in foods. Although the methodology of MALDI-MSI in food science is not yet fully established, the versatility of MALDI-MSI is expected to open a new frontier in food science. Herein, we describe the principles and applications of MALDI-MSI in food science and related fields.
  • Inhibitory Activities of Sesame Seed Extract and its Constituents against beta-Secretase, Matsumura Shinichi, Murata Kazuya, Zaima Nobuhiro, Yoshioka Yuri, Morimoto Masanori, Matsuda Hideaki, Iwaki Masahiro, NATURAL PRODUCT COMMUNICATIONS, 11, 11, 1671, 1674,   2016年11月, 査読有り
  • Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium, Nobuhiro Zaima, Saori Kinoshita, Nao Hieda, Hirona Kugo, Kaori Narisawa, Ayami Yamamoto, Kenichi Yanagimoto, Tatsuya Moriyama, Biochemistry and Biophysics Reports, 7, 259, 265,   2016年09月01日
    概要:© 2016 The Authors. Low levels of serum testosterone are characteristically associated with diabetes, coronary atherosclerosis, obstructive sleep apnea, rheumatoid arthritis, and chronic obstructive pulmonary disease. Testosterone replacement therapy is effective against many of these disorders, indicating the importance of maintaining a healthy testosterone level. In this study, we investigated the effects of fish oil on murine testosterone metabolism and analyzed the dynamics of relevant lipids in testes by matrix-assisted laser desorption ionization mass spectrometry imaging. Testosterone was upregulated in mice that received fish oil. In the testicular interstitium, eicosapentaenoic acid-containing phosphatidylcholine was distributed characteristically. These data suggest that eicosapentaenoic acid is involved in testosterone metabolism.
  • The anti-obesity effect of Taheebo (Tabebuia avellanedae Lorentz ex Griseb) extract in ovariectomized mice and the identification of a potential anti-obesity compound., Iwamoto K, Fukuda Y, Tokikura C, Noda M, Yamamoto A, Yamamoto M, Yamashita M, Zaima N, Iida A, Moriyama T, Biochemical and biophysical research communications, 478, 3, 1136, 1140,   2016年09月, 査読有り
  • 「アレルギー疾患の発症・増悪の予防-最新のアプローチ-」に寄せる 競合ELISA法による大豆アレルゲンGly m4の定量の試み, 花房 佳世, 植田 啄也, 矢野 えりか, 財満 信宏, 森山 達哉, アレルギーの臨床, 36, 9, 874, 878,   2016年08月
    概要:花粉症と関連する大豆食品による口腔アレルギー症候群の主要原因抗原としてGly m4が知られている。今回、このGly m4の競合ELISA定量系を構築し、各種豆乳や豆腐におけるGly m4を定量した。その結果、タンパク質濃度の高い豆乳ではGly m4濃度が高く、タンパク質濃度の低いものではGly m4濃度も低いという結果が得られ、豆乳においてはタンパク質濃度とGly m4濃度は相関することが明らかとなった。よって、豆乳類のOASリスクは、概ねタンパク質濃度を参考にすることで推測できる可能性が示唆された。(著者抄録)
  • The preventive effect of fish oil on abdominal aortic aneurysm development., Kugo H, Zaima N, Mouri Y, Tanaka H, Yanagimoto K, Urano T, Unno N, Moriyama T, Bioscience, biotechnology, and biochemistry, 80, 6, 1, 6,   2016年03月, 査読有り
  • Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm., Kugo H, Zaima N, Tanaka H, Mouri Y, Yanagimoto K, Hayamizu K, Hashimoto K, Sasaki T, Sano M, Yata T, Urano T, Setou M, Unno N, Moriyama T, Scientific reports, 6, 31268,   2016年08月, 査読有り
  • Inhibitory activities of sesame seed extract and its constituents against β-secretase, Shinichi Matsumura, Kazuya Murata, Nobuhiro Zaima, Yuri Yoshioka, Masanori Morimoto, Hideaki Matsuda, Masahiro Iwaki, Masahiro Iwaki, Natural Product Communications, 11, 1671, 1674,   2016年01月01日
    概要:The need for a preventive agent against dementia led us to screen natural plant resources. Among the herbs and spices tested, sesame seed prepared from Sesamum indicum seeds showed potent β-secretase inhibitory activity. The active principles were determined to be sesamin and sesamolin, typical lignans in S. indicum. The IC 50 values of sesamin and sesamolin were 257 and 140 μM, respectively. These compounds were investigated in a preliminary absorption experiment. After oral administration, these compounds were detected in an intact form in the brain and serum. These results suggest that consumption of sesame seeds may prevent dementia by sesamin and sesamolin, the constituents in sesame seeds.
  • Inhibitory activities of essential oil obtained from turmeric and its constituents against β-secretase, Shinichi Matsumura, Kazuya Murata, Nobuhiro Zaima, Yuri Yoshioka, Masanori Morimoto, Hirona Kugo, Ayami Yamamoto, Tatsuya Moriyama, Hideaki Matsuda, Natural Product Communications, 11, 1785, 1788,   2016年01月01日
    概要:The need for a preventive agent against dementia led us to screen natural plant resources. Among the herbs and spices tested, turmeric, from rhizomes of Curcuma longa, showed high potency against β-secretase. The active principles were determined as α-turmerone, β-turmerone and ar-turmerone, with IC 50 values of 39, 62 and 92 μM respectively. In this study, the efficiency of collecting the essential oil using steam distillation of the volatile substance was disclosed. The active principles were explored, and four sesquiterpenoids and five monoterpenoids were revealed as active principles against β-secretase. On the other hand, α-turmerone, β-turmerone and ar-turmerone were also investigated in a pharmacokinetic absorption experiment. After oral administration, these compounds were detected in an intact form in the brain and serum. These results suggest that consumption of turmeric constituents may prevent dementia.
  • Mass spectrometry based chemical imaging of foods, Shekhar U. Kadam, N. N. Misra, Nobuhiro Zaima, RSC Advances, 6, 33537, 33546,   2016年01月01日
    概要:© The Royal Society of Chemistry 2016. Chemical imaging based on mass spectrometry is an emerging technology which has opened opportunities for fundamental research in food science. The ability to quantitatively determine the spatial distribution of several chemical entities simultaneously makes it a method of choice for chemical characterization of plant materials and foods. In this review, an overview of the ionization methods is presented, followed by a discussion of the current state of the art in mass spectrometry imaging (MSI) of relevance to practices in food research. The known applications, analytical challenges, and future research potential are highlighted. MSI has been successfully utilized to a variable degree for obtaining information about spatial distribution of a variety of molecules and metabolites in foods. It allows visualizing the spatial molecular maps without extraction, purification, separation, or labelling of foods. The method is likely to find wider adoption with developments in ionization sources and inter-laboratory collaborations.
  • Anti-Hyperglycemic Effect of a Kudzu (Pueraria lobata) Vine Extract in Ovariectomized Mice., Tanaka T, Yokota Y, Tang H, Zaima N, Moriyama T, Kawamura Y, Journal of nutritional science and vitaminology, 62, 5, 341, 349,   2016年, 査読有り
  • Insufficient Lymph Drainage Causes Abnormal Lipid Accumulation and Vein Wall Degeneration., Tanaka H, Yamamoto N, Suzuki M, Mano Y, Sano M, Zaima N, Sasaki T, Setou M, Unno N, Annals of vascular diseases, 9, 4, 277, 284,   2016年, 査読有り
  • Characteristic Distribution Pattern of Lysophosphatidylcholine in Fibromuscular Dysplasia-Associated Visceral Artery Aneurysms Compared with Atherosclerotic Visceral Artery Aneurysms., Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Sano M, Konno H, Urano T, Setou M, Unno N, Journal of atherosclerosis and thrombosis,   2015年12月, 査読有り
  • Distinct cardiac phenotype between two homozygotes born in a village with accumulation of a genetic deficiency of adipose triglyceride lipase., Higashi M, Hirano K, Kobayashi K, Ikeda Y, Issiki A, Otsuka T, Suzuki A, Yamaguchi S, Zaima N, Hamada S, Hanada H, Suzuki C, Nakamura H, Nagasaka H, Miyata T, Miyamoto Y, Kobayashi K, Naito H, Toda T, International journal of cardiology, 192, 30, 32,   2015年08月, 査読有り
  • Hypoperfusion of the Adventitial Vasa Vasorum Develops an Abdominal Aortic Aneurysm., Tanaka H, Zaima N, Sasaki T, Sano M, Yamamoto N, Saito T, Inuzuka K, Hayasaka T, Goto-Inoue N, Sugiura Y, Sato K, Kugo H, Moriyama T, Konno H, Setou M, Unno N, PloS one, 10, 8, e0134386,   2015年, 査読有り
  • Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall., Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Sano M, Saito T, Hayasaka T, Goto-Inoue N, Sato K, Kugo H, Moriyama T, Konno H, Setou M, Unno N, Journal of vascular research, 52, 2, 127, 135,   2015年, 査読有り
  • DHA-PC and PSD-95 decrease after loss of synaptophysin and before neuronal loss in patients with Alzheimer's disease., Yuki D, Sugiura Y, Zaima N, Akatsu H, Takei S, Yao I, Maesako M, Kinoshita A, Yamamoto T, Kon R, Sugiyama K, Setou M, Scientific reports, 4, 7130,   2014年11月, 査読有り
  • Enhanced specificity for phosphatidylcholine analysis by positive ion mode matrix-assisted laser desorption/ionization imaging mass spectrometry., Zaima N, Yoshioka S, Sato Y, Shinano S, Ikeda Y, Moriyama T, Rapid communications in mass spectrometry : RCM, 28, 13, 1453, 1458,   2014年07月, 査読有り
  • Distribution of lysophosphatidylcholine in the endosperm of Oryza sativa rice., Zaima N, Yoshimura Y, Kawamura Y, Moriyama T, Rapid communications in mass spectrometry : RCM, 28, 13, 1515, 1520,   2014年07月, 査読有り
  • Coronary triglyceride deposition in contemporary advanced diabetics., Ikeda Y, Zaima N, Hirano K, Mano M, Kobayashi K, Yamada S, Yamaguchi S, Suzuki A, Kanzaki H, Hamasaki T, Kotani J, Kato S, Nagasaka H, Setou M, Ishibashi-Ueda H, Pathology international, 64, 7, 325, 335,   2014年07月, 査読有り
  • Molecular cloning and expression profiling of procollagen alpha 1 (I) of cultured Pacific bluefin tuna, Tanaka Teruyoshi, Takahashi Kenji, Adachi Kohsuke, Ohta Haruki, Yoshimura Yukihiro, Agawa Yasuo, Sawada Yoshifumi, Takaoka Osamu, Biswas Amal Kumar, Takii Kenji, Zaima Nobuhiro, Moriyama Tatsuya, Kawamura Yukio, FISHERIES SCIENCE, 80, 3, 603, 612,   2014年05月, 査読有り
  • Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm, Masaki Sano, Masaki Sano, Takeshi Sasaki, Satoshi Hirakawa, Junichi Sakabe, Mikako Ogawa, Satoshi Baba, Nobuhiro Zaima, Nobuhiro Zaima, Hiroki Tanaka, Hiroki Tanaka, Kazunori Inuzuka, Kazunori Inuzuka, Naoto Yamamoto, Naoto Yamamoto, Mitsutoshi Setou, Kohji Sato, Hiroyuki Konno, Naoki Unno, Naoki Unno, PLoS ONE, 9,   2014年03月20日
    概要:The pathogenesis of abdominal aortic aneurysm (AAA) is characterized to be inflammation-associated degeneration of vascular wall. Neovascularization is regularly found in human AAA and considered to play critical roles in the development and rupture of AAA. However, little is known about lymphangiogenesis in AAA. The purpose of this study was to demonstrate both angiogenesis and lymphangiogenesis in AAA. Abdominal aortic tissue was harvested either from autopsy (control group) and during open-repair surgery for AAA (AAA group). Adventitial lymphatic vasa vasorum was observed in both groups, but seemed to be no significant morphological changes in AAA. Immunohistochemical studies identified infiltration of lymphatic vessel endothelial hyaluronan receptor (LYVE) -1, vascular endothelial growth factor (VEGF)-C, and matrix metalloproteinase (MMP)-9-positive macrophages and podoplanin and Prox-1-positive microvessels in the intima/media in AAA wall, where hypoxia-inducible factors (HIF)-1α was expressed. VEGF-C and MMP-9 were not expressed in macrophages infiltrating in the adventitia. Intraoperative indocyanine green fluorescence lymphography revealed lymph stasis in intima/medial in AAA. Fluorescence microscopy of the collected samples also confirmed the accumulation of lymph in the intima/media but not in adventitia. These results demonstrate that infiltration of macrophages in intima/media is associated with lymphangiogenesis and angiogenesis in AAA. Lymph-drainage appeared to be insufficient in the AAA wall. © 2014 Sano et al.
  • Genetic mutations in adipose triglyceride lipase and myocardial up-regulation of peroxisome proliferated activated receptor-γ in patients with triglyceride deposit cardiomyovasculopathy, Ken Ichi Hirano, Ken Ichi Hirano, Tatsuya Tanaka, Yoshihiko Ikeda, Satoshi Yamaguchi, Satoshi Yamaguchi, Nobuhiro Zaima, Kazuhiro Kobayashi, Akira Suzuki, Akira Suzuki, Yasuhiko Sakata, Yasuhiko Sakata, Yasushi Sakata, Kunihisa Kobayashi, Tatsushi Toda, Norihide Fukushima, Hatsue Ishibashi-Ueda, Daniela Tavian, Hironori Nagasaka, Shu Ping Hui, Hitoshi Chiba, Yoshiki Sawa, Masatsugu Hori, Biochemical and Biophysical Research Communications, 443, 574, 579,   2014年01月10日
    概要:Adipose triglyceride lipase (ATGL, also known as PNPLA2) is an essential molecule for hydrolysis of intracellular triglyceride (TG). Genetic ATGL deficiency is a rare multi-systemic neutral lipid storage disease. Information regarding its clinical profile and pathophysiology, particularly for cardiac involvement, is still very limited. A previous middle-aged ATGL-deficient patient in our institute (Case 1) with severe heart failure required cardiac transplantation (CTx) and exhibited a novel phenotype, "Triglyceride deposit cardiomyovasculopathy (TGCV)". Here, we tried to elucidate molecular mechanism underlying TGCV. The subjects were two cases with TGCV, including our second case who was a 33-year-old male patient (Case 2) with congestive heart failure requiring CTx. Case 2 was homozygous for a point mutation in the 5′ splice donor site of intron 5 in the ATGL, which results in at least two types of mRNAs due to splicing defects. The myocardium of both patients (Cases 1 and 2) showed up-regulation of peroxisome proliferated activated receptors (PPARs), key transcription factors for metabolism of long chain fatty acids (LCFAs), which was in contrast to these molecules' lower expression in ATGL-targeted mice. We investigated the intracellular metabolism of LCFAs under human ATGL-deficient conditions using patients' passaged skin fibroblasts as a model. ATGL-deficient cells showed higher uptake and abnormal intracellular transport of LCFA, resulting in massive TG accumulation. We used these findings from cardiac specimens and cell-biological experiments to construct a hypothetical model to clarify the pathophysiology of the human disorder. In patients with TGCV, even when hydrolysis of intracellular TG is defective, the marked up-regulation of PPARγ and related genes may lead to increased uptake of LCFAs, the substrates for TG synthesis. This potentially vicious cycle of LCFAs could explain the massive accumulation of TG and severe clinical course for this rare disease. © 2013 Elsevier Inc. All rights reserved.
  • Matrix-assisted laser desorption/ionization imaging mass spectrometry: new technology for vascular pathology., Zaima N, Goto-Inoue N, Moriyama T, Journal of vascular research, 51, 2, 144, 148,   2014年, 査読有り
  • Hypoallergenicity of various miso pastes manufactured in Japan, Tatsuya Moriyama, Erika Yano, Yuusuke Suemori, Kyoko Nakano, Nobuhiro Zaima, Yukio Kawamura, Journal of Nutritional Science and Vitaminology, 59, 462, 469,   2013年12月01日
    概要:Miso paste (miso), a fermented soybean food, is popular in Japan and other Asian countries. However, the soybean is known to induce an allergenic response in some individuals. In the present study, we evaluated the allergenicity of various kinds of miso available in Japan. Total proteins were extracted from Amakuti-kome miso, Karakuti-kome miso, Mugi-miso and Mame-miso, and the protein profiles were analyzed. The major protein bands detected in the intact soybean extract were not present in any of the miso samples, which instead showed various low molecular weight protein bands of approximately 10-25 kDa. The existence levels of six major soybean allergens were determined by Western blotting using specific antibodies. We found that the allergen levels varied among miso and allergen types; however, allergen levels were consistently lower in miso than in the soybean extract. We obtained similar results for IgE-ELISA experiments using serum IgE from soybean allergy patients. Taken together, these results indicate that compared to soybean extract, various types of miso contain small quantities of intact soybean allergens. Additionally, several lines of evidence indicated that the allergen levels were exceptionally low in the dark-colored Karakuti-kome miso and Mame-miso, which are produced with relatively long fermentation periods, suggesting that the duration of fermentation might be a key factor in the hypoallergenicity of miso.
  • Cilostazol inhibits accumulation of triglycerides in a rat model of carotid artery ligation, Hiroki Tanaka, Hiroki Tanaka, Hiroki Tanaka, Nobuhiro Zaima, Nobuhiro Zaima, Hideki Ito, Katsuji Hattori, Naoto Yamamoto, Naoto Yamamoto, Hiroyuki Konno, Mitsutoshi Setou, Naoki Unno, Naoki Unno, Journal of Vascular Surgery, 58, 1366, 1374,   2013年11月01日
    概要:Objective: Triglyceride (TG) accumulation in arterial tissue is associated with the development of cardiovascular disease; however, the underlying mechanism remains unclear. Cilostazol (CLZ), a selective inhibitor of phosphodiesterase 3, has antiplatelet and vasodilating effects and may decrease serum TG levels. We examined the effect of CLZ on TG accumulation in the arterial tissue of a rat model of carotid artery ligation. Methods: Rats were fed normal chow with 0.1% CLZ (CLZ group) or without CLZ (control group) for 4 weeks after unilateral carotid artery ligation near the carotid bifurcation. Results: At the end of this period, the control group showed 3.3-fold higher TG levels in the ligated carotid artery than in the contralateral artery; however, compared with the contralateral artery, the ligated artery in the CLZ group showed significantly lower levels of TG accumulation but similar serum levels of TG, total cholesterol, and high-density lipoprotein cholesterol. Furthermore, matrix-assisted laser desorption/ionization imaging mass spectrometry revealed that the ligated carotid artery in both groups had ubiquitous accumulation of TG in the intima, media, and adventitia, along with decreased heme B signals, which was indicative of ischemia. However, heme B signals were less reduced in the CLZ group than in the control group. Conclusions: Our results indicate that CLZ can inhibit the ubiquitous accumulation of TG in arterial tissues, possibly by ameliorating tissue ischemia. CLZ may be useful in improving arterial tissue hemodynamics and lipid metabolism. © 2013 by the Society for Vascular Surgery.
  • Ellagic acid improves hepatic steatosis and serum lipid composition through reduction of serum resistin levels and transcriptional activation of hepatic ppara in obese, diabetic KK-A(y) mice, Yoshimura Yukihiro, Nishii Saori, Zaima Nobuhiro, Moriyarha Tatsuya, Kawamura Yukio, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 434, 3, 486, 491,   2013年05月10日, 査読有り
  • Adventitial Vasa Vasorum Arteriosclerosis in Abdominal Aortic Aneurysm, Tanaka Hiroki, Zaima Nobuhiro, Sasaki Takeshi, Hayasaka Takahiro, Goto-Inoue Naoko, Onoue Kenji, Ikegami Koji, Morita Yoshifumi, Yamamoto Naoto, Mano Yuuki, Sano Masaki, Saito Takaaki, Sato Kohji, Konno Hiroyuki, Setou Mitsutoshi, Unno Naoki, PLOS ONE, 8, 2,   2013年02月27日, 査読有り
  • Visualization of alpha-tocopherol in biological samples using imaging mass spectrometry, Nobuhiro Zaima, Itsuko Ishizaki, Tocopherol: Sources, Uses and Health Benefits, 35, 43,   2012年12月01日
    概要:Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and secondary ion mass spectrometry (SIMS) are powerful tools for visualization of alpha-tocopherol in biological samples. In this review, we describe the methodology and applications of imaging mass spectrometry based on MALDI-MS (MALDI-IMS) and time-of-flight SIMS (TOF-SIMS) for visualization of alpha-tocopherol in biological samples. © 2012 by Nova Science Publishers, Inc. All rights reserved.
  • Application of matrix-assisted laser desorption/ionization imaging mass spectrometry for the analysis of Oryza sativa rice, Nobuhiro Zaima, Rice: Production, Consumption and Health Benefits, 175, 182,   2012年12月01日
    概要:Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without the need for extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields. In this review, the methodology and applications of MALDI-IMS for analysis of rice samples are described. © 2012 by Nova Science Publishers, Inc. All rights reserved.
  • Imaging Mass Spectrometry Visualizes Ceramides and the Pathogenesis of Dorfman-Chanarin Syndrome Due to Ceramide Metabolic Abnormality in the Skin, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Kimiko Nakajima, Walter M. Holleran, Shigetoshi Sano, Yoshikazu Uchida, Mitsutoshi Setou, PLoS ONE, 7,   2012年11月15日
    概要:Imaging mass spectrometry (IMS) is a useful cutting edge technology used to investigate the distribution of biomolecules such as drugs and metabolites, as well as to identify molecular species in tissues and cells without labeling. To protect against excess water loss that is essential for survival in a terrestrial environment, mammalian skin possesses a competent permeability barrier in the stratum corneum (SC), the outermost layer of the epidermis. The key lipids constituting this barrier in the SC are the ceramides (Cers) comprising of a heterogeneous molecular species. Alterations in Cer composition have been reported in several skin diseases that display abnormalities in the epidermal permeability barrier function. Not only the amounts of different Cers, but also their localizations are critical for the barrier function. We have employed our new imaging system, capable of high-lateral-resolution IMS with an atmospheric-pressure ionization source, to directly visualize the distribution of Cers. Moreover, we show an ichthyotic disease pathogenesis due to abnormal Cer metabolism in Dorfman-Chanarin syndrome, a neutral lipid storage disorder with ichthyosis in human skin, demonstrating that IMS is a nove l diagnostic approach for assessing lipid abnormalities in clinical setting, as well as for investigating physiological roles of lipids in cells/tissues. © 2012 Goto-Inoue et al.
  • Hepatoprotective action of dietary bluefin tuna skin proteins on CCl 4-intoxicated mice, Teruyoshi Tanaka, Kenji Takahashi, Naoki Iwamoto, Yasuo Agawa, Yoshifumi Sawada, Yukihiro Yoshimura, Nobuhiro Zaima, Tatsuya Moriyama, Yukio Kawamura, Fisheries Science, 78, 911, 921,   2012年07月01日
    概要:We have shown that dietary bluefin tuna skin (TUS) protects against carbon tetrachloride (CCl 4 )-induced hepatic damage in mice. The CCl 4 -induced necrotic area was decreased in mice fed a TUS-containing diet. Consistent with the decreased necrotic area, dietary TUS markedly lowered the elevated serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and the thiobarbituric acid-reactive substance (TBARS) formation induced by CCl 4 injection. TUS diets also decreased phosphorylation of inhibitory kappa B-α and blocked the translocation of nuclear factor-kappa B to the nucleus. TUS is composed mainly (80.7 %) of type I collagen, and our results revealed that dietary tuna collagen peptides (TUCP) attenuated the increased hepatic necrotic area, serum AST and ALT activities, and liver TBARS levels induced by CCl 4 , similar to TUS, thus enabling us to attribute the hepatoprotective action of TUS in CCl 4 -intoxicated mice to tuna collagen. Therefore, TUS and TUCP may be potential food resources that are capable of alleviating hepatitis symptoms. © 2012 The Japanese Society of Fisheries Science.
  • Transcriptome analysis of the duodenum, pancreas, liver, and muscle from diabetic goto-kakizaki rats fed a trypsin inhibitor derived from squid viscera, Kohsuke Adachi, Kohsuke Adachi, Kana Fukumorita, Michihiro Araki, Nobuhiro Zaima, Zhi Hong Yang, Satoru Chiba, Hideki Kishimura, Hiroki Saeki, Journal of Agricultural and Food Chemistry, 60, 5540, 5546,   2012年06月06日
    概要:Trypsin inhibitors (TIs) have various nutritional effects. However, a detailed mechanism for their effects, especially on the gene expression patterns in various tissues, remains unknown. Here, we used transcriptome techniques and gene ontology (GO) analysis to examine the effects of squid TI (sqTI), a biochemically stable peptide, on diabetic Goto-Kakizaki rats after feeding for 10 weeks. We demonstrated that downregulation of SREBP1c in the liver via duodenal/pancreatic hormones suppresses the blood cholesterol level. Consistently, in GO analysis, the term "cholesterol biosynthetic process" was enriched among downregulated genes. No hypoglycemic or insulinotropic effects were observed, in contrast to the results from our previous studies (single stimulation with the same dose of TI), which can be partly ascribed to the inactive responses of the duodenum and pancreas in this condition. © 2012 American Chemical Society.
  • Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry, Yuki Sugiura, Yuki Sugiura, Nobuhiro Zaima, Mitsutoshi Setou, Seiji Ito, Ikuko Yao, Ikuko Yao, Analytical and Bioanalytical Chemistry, 403, 1851, 1861,   2012年06月01日
    概要:Metabolite distribution imaging via imaging mass spectrometry (IMS) is an increasingly utilized tool in the field of neurochemistry. As most previous IMS studies analyzed the relative abundances of larger metabolite species, it is important to expand its application to smaller molecules, such as neurotransmitters. This study aimed to develop an IMS application to visualize neurotransmitter distribution in central nervous system tissue sections. Here, we raise two technical problems that must be resolved to achieve neurotransmitter imaging: (1) the lower concentrations of bioactive molecules, compared with those of membrane lipids, require higher sensitivity and/or signal-to-noise (S/N) ratios in signal detection, and (2) the molecular turnover of the neurotransmitters is rapid; thus, tissue preparation procedures should be performed carefully to minimize postmortem changes. We first evaluated intrinsic sensitivity and matrix interference using Matrix Assisted Laser Desorption/ Ionization (MALDI) mass spectrometry (MS) to detect six neurotransmitters and chose acetylcholine (ACh) as a model for study. Next, we examined both single MS imaging and MS/MS imaging for ACh and found that via an ion transition from m/z146 to m/z87 in MS/MS imaging, ACh could be visualized with a high S/N ratio. Furthermore, we found that in situ freezing method of brain samples improved IMS data quality in terms of the number of effective pixels and the image contrast (i.e., the sensitivity and dynamic range). Therefore, by addressing the aforementioned problems, we demonstrated the tissue distribution of ACh, the most suitable molecular specimen for positive ion detection by IMS, to reveal its localization in central nervous system tissues. © The Author(s) 2012.
  • Visualization of anthocyanin species in rabbiteye blueberry vaccinium ashei by matrix-assisted laser desorption/ionization imaging mass spectrometry, Yukihiro Yoshimura, Hirofumi Enomoto, Tatsuya Moriyama, Yukio Kawamura, Mitsutoshi Setou, Nobuhiro Zaima, Analytical and Bioanalytical Chemistry, 403, 1885, 1895,   2012年06月01日
    概要:Anthocyanins are naturally occurring compounds that impart color to fruits, vegetables, and plants, and are believed to have a number of beneficial health effects in both humans and animals. Because of these properties, pharmacokinetic analysis of anthocyanins in tissue has been performed to quantify and identify anthocyanin species although, currently, no methods exist for investigating tissue localization of anthocyanin species or for elucidating the mechanisms of anthocyanin activity. Imaging mass spectrometry (IMS) is powerful tool for determining and visualizing the distribution of a wide range of biomolecules. To investigate whether anthocyanin species could be identified and visualized by IMS, we performed matrix-assisted laser desorption/ionization (MALDI)-IMS analysis, by tandem mass spectrometry (MALDI-IMS-MS), of ten anthocyanin molecular species in rabbiteye blueberry (Vaccinium ashei). The distribution patterns of each anthocyanin species were different in the exocarp and endocarp of blueberry sections. Anthocyanin species composed of delphinidin and petunidin were localized mainly in the exocarp. In contrast, those species composed of cyanidin, peonidin, and malvidin were localized in both the exocarp and the endocarp. Moreover, MALDI-IMS analysis of anthocyanidins in a blueberry section indicated that the distribution patterns of each anthocyanidin species were nearly identical with those of the corresponding anthocyanins. These results suggested that the different distribution patterns of anthocyanin species in the exocarp and endocarp depended on the aglycone rather than on the sugar moieties. This study is the first to visualize anthocyanin molecular species in fruits. © Springer-Verlag 2012.
  • Imaging mass spectrometry reveals changes of metabolites distribution in mouse testis during testicular maturation, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Mitsutoshi Setou, Surface and Interface Analysis, 44, 749, 754,   2012年06月01日
    概要:Although lipid metabolism is thought to be important for proper testicular maturation, the molecular mechanisms underlying this dynamic process remain incompletely understood. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) can generate molecular ion images of tissue surfaces with high sensitivities and minimal chemical damage. With a wide range of detectable targets, we visualized various kinds of metabolites, such as glutathione, UDP-HexNAc, phosphatidylcholine, sphingomyelin, phosphatidylinositol, seminolipid, and sulfatide. Here, we demonstrate the use of MALDI-IMS with frozen sections of mouse testis at different developmental stages and clearly reveal that some molecular species of phosphatidylcholines and seminolipids show stage-specific expression in testis. The result suggested that these molecular species serve different cellular functions during maturation. Copyright © 2011 John Wiley & Sons, Ltd. Copyright © 2011 John Wiley & Sons, Ltd.
  • Loss of lymphatic vessels and regional lipid accumulation is associated with great saphenous vein incompetence, Hiroki Tanaka, Hiroki Tanaka, Hiroki Tanaka, Nobuhiro Zaima, Nobuhiro Zaima, Takeshi Sasaki, Naoto Yamamoto, Naoto Yamamoto, Masaki Sano, Masaki Sano, Hiroyuki Konno, Mitsutoshi Setou, Naoki Unno, Naoki Unno, Journal of Vascular Surgery, 55, 1440, 1448,   2012年05月01日
    概要:Objective: Recent studies suggest that biologic changes in the vein wall associated with varicose veins (VVs) occur not only in v alvular tissue but also in nonvalvular regions. We previously used imaging mass spectrometry (IMS) to determine the distribution of lipid molecules in incompetent valve tissue. In this study, we used IMS to analyze incompetent great saphenous veins (GSVs) in patients with varicose vein (VV) to assess the distribution of lipid molecules. Methods: We obtained GSV tissue from 38 VV patients (50 limbs) who underwent GSV stripping. For the control veins (CV), we obtained GSV samples from 10 patients undergoing infrainguinal bypass with reversed GSV grafting for peripheral artery occlusive disease (10 limbs). Conventional and immunofluorescence staining were performed for histopathologic examination. The total lipid content in the homogenized vein tissue was determined. The localization of each lipid molecule in the vein wall was assessed by IMS. Results: The histologic examination showed the VV walls were significantly thicker than the CV walls, and only the VV adventitia was positive for lipid staining. The VV wall had higher concentrations of phospholipids and triglycerides than the CV wall. IMS revealed an abnormal accumulation of lysophosphatidylcholine (LPC; 1-acyl 16:0) and phosphatidylcholine (diacyl 16:0/20:4) in the VV intima and media. Triglyceride was found only in VV adventitia. The number of lymphatic vessels, as measured by staining with D2-40, a lymphatic vessel-specific marker, was significantly lower in the VV adventitia than in the CV adventitia. Lymphatic vessel reduction may be associated with insufficient lymphatic drainage in the VV adventitia causing histologic changes in VV tissue. Conclusions: The accumulation of LPC (1-acyl 16:0) and PC (diacyl 16:0/20:4) in the VV intima and media may be associated with chronic inflammation, leading to VV tissue degeneration. Furthermore, insufficient lipid drainage by lymphatic vessel may be responsible for accumulation of lipid molecules and subsequent vein wall degeneration. © 2012 Society for Vascular Surgery.
  • Composition and localization of lipids in penaeus merguiensis ovaries during the ovarian maturation cycle as revealed by imaging mass spectrometry, Piyachat Chansela, Piyachat Chansela, Naoko Goto-Inoue, Nobuhiro Zaima, Takahiro Hayasaka, Morakot Sroyraya, Morakot Sroyraya, Napamanee Kornthong, Attakorn Engsusophon, Montakan Tamtin, Chatchawalee Chaisri, Prasert Sobhon, Mitsutoshi Setou, PLoS ONE, 7,   2012年03月14日
    概要:Ovary maturation, oocyte differentiation, and embryonic development in shrimp are highly dependent on nutritional lipids taken up by female broodstocks. These lipids are important as energy sources as well as for cell signaling. In this study, we report on the compositions of major lipids, i.e. phosphatidylcholines (PCs), triacylglycerols (TAGs), and fatty acids (FAs), in the ovaries of the banana shrimp, Penaeus merguiensis, during ovarian maturation. Thin-layer chromatography analysis showed that the total PC and TAG signal intensities increased during ovarian maturation. Further, by using gas chromatography, we found that (1) FAs 14:0, 16:1, 18:1, 18:2, 20:1, and 22:6 proportionally increased as ovarian development progressed to more mature stages; (2) FAs 16:0, 18:0, 20:4, and 20:5 proportionally decreased; and (3) FAs 15:0, 17:0, and 20:2 remained unchanged. By using imaging mass spectrometry, we found that PC 16:0/16:1 and TAG 18:1/18:2/22:6 were detected in oocytes stages 1 and 2. PCs 16:1/20:4, 16:0/22:6, 18:3/22:6, 18:1/22:6, 20:5/22:6, and 22:6/22:6 and TAGs 16:0/16:1/18:3, 16:0/18:1/18:3, 16:0/18:1/18:1, and 16:0/18:2/22:6 were present in all stages of oocytes. In contrast, the PC- and TAG-associated FAs 20:4, 20:5, and 22:6 showed high signal intensities in stage 3 and 4 oocytes. These FAs may act as nutrition sources as well as signaling molecules for developing embryos and the hatching process. Knowledge of lipid compositions and localization could be helpful for formulating the diet for female broodstocks to promote fecundity and larval production. © 2012 Chansela et al.
  • Visualization of neuropeptides in paraffin-embedded tissue sections of the central nervous system in the decapod crustacean, Penaeus monodon, by imaging mass spectrometry, Piyachat Chansela, Piyachat Chansela, Naoko Goto-Inoue, Nobuhiro Zaima, Nobuhiro Zaima, Morakot Sroyraya, Morakot Sroyraya, Prasert Sobhon, Mitsutoshi Setou, Peptides, 34, 10, 18,   2012年03月01日
    概要:The distributions of neuropeptides in paraffin-embedded tissue sections (PETS) of the eyestalk, brain, and thoracic ganglia of the shrimp Penaeus monodon were visualized by imaging mass spectrometry (IMS). Peptide signals were obtained from PETS without affecting morphological features. Twenty-nine neuropeptides comprising members of FMRFamide, SIFamides, crustacean hyperglycaemic hormone, orcokinin-related peptides, tachykinin-related peptides, and allatostatin A were detected and visualized. Among these findings we first identified tachykinin-related peptide as a novel neuropeptide in thi s shrimp species. We found that these neuropeptides were distributed at specific areas in the three neural organs. In addition, 28 peptide sequences derived from 4 types of constitutive proteins, including actin, histones, arginine kinase, and cyclophilin A were also detected. All peptide sequences were verified by liquid chromatography-tandem mass spectrometry. The use of IMS on acetic acid-treated PETS enabled us to identify peptides and obtain their specific localizations in correlation with the undisturbed histological structure of the tissue samples. © 2011 Elsevier Inc. All rights reserved.
  • Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry, Yukihiro Yoshimura, Nobuhiro Zaima, Tatsuya Moriyama, Yukio Kawamura, PLoS ONE, 7,   2012年02月17日
    概要:Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS. © 2012 Yoshimura et al.
  • Ellagic acid in pomegranate suppresses resistin secretion by a novel regulatory mechanism involving the degradation of intracellular resistin protein in adipocytes, Makino-Wakagi Yasuko, Yoshimura Yukihiro, Uzawa Yuki, Zaima Nobuhiro, Moriyama Tatsuya, Kawamura Yukio, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 417, 2, 880, 885,   2012年01月13日, 査読有り
  • Kudzu (Pueraria lobata) vine ethanol extracts improve ovariectomy-induced bone loss in female mice, Teruyoshi Tanaka, Hanjun Tang, Fengnian Yu, Seiwa Michihara, Yuki Uzawa, Nobuhiro Zaima, Tatsuya Moriyama, Yukio Kawamura, Journal of Agricultural and Food Chemistry, 59, 13230, 13237,   2011年12月28日
    概要:Bone-loss-improving action of kudzu vine ethanol extracts (PVEE) was clarified. PVEE was composed roughly of 80% fiber, 10% puerarin, 3.6% daidzin, 2.5% 6″-O-malonyldaidzin, and the other minor isoflavones. Ten-week-old ovariectomized (OVX) mice were fed diets containing PVEE (20 mg/kg body weight/day) for 8 weeks. The bone resorption markers (urinary deoxypyridinoline and tartrate-resistant acid phosphatase activity) was elevated in OVX mice and was significantly decreased in OVX mice that consumed PVEE for 8 weeks. Consistent with the decrease in the markers, the number of matured osteoclasts in the distal femur was diminished in OVX mice fed PVEE diets. PVEE diets also suppressed the decrease in femoral bone mineral density (BMD) by OVX. PVEE showed the affinity for estrogen receptor α and β nearly 1/10000 weaker than 17β-estradiol. No hypertrophy in the uterus by the PVEE diet was observed. These results suggest that PVEE could be a promising resource for a functional food that improves osteoporosis. © 2011 American Chemical Society.
  • Imaging mass spectrometry for lipidomics, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Mitsutoshi Setou, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1811, 961, 969,   2011年11月01日
    概要:Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-MS technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. This technique can reveal the distribution of hundreds of ion signals in a single measurement and also helps in understanding the cellular profile of the biological system. MALDI-IMS has already revealed the characteristic distrib ution of several kinds of lipids in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields, especially in lipidomics. In this review, we describe the methodology and applications of MALDI-IMS to biological samples. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrometry. © 2011 Elsevier B.V.
  • Investigation by Imaging Mass Spectrometry of Biomarker Candidates for Aging in the Hair Cortex, Waki Michihiko Luca, Onoue Kenji, Takahashi Tsukasa, Goto Kensuke, Saito Yusuke, Inami Katsuaki, Makita Ippei, Angata Yurika, Suzuki Tomomi, Yamashita Mihi, Sato Narumi, Nakamura Saki, Yuki Dai, Sugiura Yuki, Zaima Nobuhiro, Goto-Inoue Naoko, Hayasaka Takahiro, Shimomura Yutaka, Setou Mitsutoshi, PLOS ONE, 6, 10,   2011年10月24日, 査読有り
  • Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex, D. Yuki, Y. Sugiura, N. Zaima, N. Zaima, H. Akatsu, Y. Hashizume, T. Yamamoto, M. Fujiwara, K. Sugiyama, K. Sugiyama, M. Setou, Neuroscience, 193, 44, 53,   2011年10月13日
    概要:Sulfatide (ST) is a sphingolipid with an important role in the central ner vous system as a major component of the myelin sheath. ST contains a structurally variable ceramide moiety, with a fatty acid substituent of varying carbon-chain length and double-bond number. Hydroxylation at the α-2 carbon position of the fatty acid is found in half the population of ST molecules. Recent genetic studies of fatty acid 2-hydroxylase (FA2H) indicate that these hydroxylated sphingolipids influence myelin sheath stability. However, their distribution is unknown. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) enables the analysis of distinct distributions of individual ST molecular species in tissue section. We examined human cerebral cortex tissue sections with MALDI-IMS, identifying and characterizing the distributions of 14 ST species. The distribution analysis reveals that the composition ratios of non-hydroxylated/hydroxylated STs are clearly reversed at the border between white and gray matter; the hydroxylated group is the dominant ST species in the gray matter. These results suggest that hydroxylated STs are highly expressed in oligodendrocytes in gray matter and might form stable myelin sheaths. As a clinical application, we analyzed a brain with Alzheimer's disease (AD) as a representative neurodegenerative disease. Although previous studies of AD pathology have reported that the amount of total ST is decreased in the cerebral cortex, as far as the compositional distributions of STs are concerned, AD brains were similar to those in control brains. In conclusion, we suggest that MALDI-IMS is a useful tool for analysis of the distributions of various STs and this application might provide novel insight in the clinical study of demyelinating diseases. © 2011 IBRO.
  • Transcriptome analysis of the duodenum in wistar rats fed a trypsin inhibitor derived from squid viscera, Kohsuke Adachi, Kohsuke Adachi, Kana Fukumorita, Michihiro Araki, Nobuhiro Zaima, Satoru Chiba, Hideki Kishimura, Hiroki Saeki, Journal of Agricultural and Food Chemistry, 59, 9001, 9010,   2011年08月24日
    概要:To investigate the effects of oral administration of a trypsin inhibitor (TI), normal Wistar rats were fed a TI derived from squid (Todarodes pacificus) for 10 weeks and gene expression profiles in the duodenum, pancreas, liver, and muscle were then analyzed using DNA microarrays. Although no significant changes could be observed in growth, food intake, tissue weight, or blood tests among the tissues tested, the duodenum showed the most remarkable changes in the global gene expression profile. Significant up-regulation of mRNAs encoding gastrin, gastrokine, cholecystokinin and somatostatin in the duodenum was validated by qPCR analysis. In gene ontology (GO) analysis of the up-regulated differentially expressed genes (DEGs), GO terms related to keratinization and innate mucosal defense were enriched (p < 0.001) in the category of biological processes in addition to assumable terms such as regulation of secretion and response to nutrients, vesicle-mediated transport, and so forth. In the same analysis, calcium ion binding was listed at the deepest hierarchy in the category of molecular function. These results indicate that the duodenum responds to TI treatment by a wider range of physiological processes than previously assumed such as keratinocyte differentiation and innate mucosal defense, in which calcium plays a crucial role. © 2011 American Chemical Society.
  • Imaging mass spectrometry-based histopathologic examination of atherosclerotic lesions, Nobuhiro Zaima, Nobuhiro Zaima, Takeshi Sasaki, Takeshi Sasaki, Hiroki Tanaka, Hiroki Tanaka, Xian Wu Cheng, Kenji Onoue, Takahiro Hayasaka, Naoko Goto-Inoue, Hirofumi Enomoto, Naoki Unno, Masafumi Kuzuya, Mitsutoshi Setou, Atherosclerosis, 217, 427, 432,   2011年08月01日
    概要:Aims: Imaging mass spectrometry (IMS) enables the visualization of individual molecules present on tissue sections. We attempted to identify and visualize specific markers for aortic atherosclerotic lesions. Methods and results: Atherosclerotic lesions were obtained from aortic roots of apolipoprotein E (ApoE)-deficient mice at 60 weeks of age and from femoral arteries of humans with peripheral artery occlusive disease. IMS was performed with a matrix-assisted laser desorption/ionization mass spectrometry time-of-flight (TOF)/TOF-type instrument. The molecular ions at m/. z 671.6 and 673.6 were found to be specific molecules in the mouse and human lipid-rich regions. These molecules were assigned as cholesterol linoleate (CE 18:2) and cholesterol oleate (CE 18:1). In the case of the human samples, triacylglycerol was also localized in the lipid-rich regions. The distributions of the molecular ions at m/. z 804.5 and 832.5 were the same as the distribution of both the mouse and the human SMCs. These molecules were assigned as phosphatidylcholine (PC) (diacyl 16:0/20:4) and PC (diacyl 18:0/20:4). The molecular ion at m/. z 566.9 was localized in the mouse calcified regions, and the molecular ions at m/. z 539.0 were localized in the human calcified regions. Conclusions: The IMS-based histopathologic examination (IbHE) revealed the characteristic peaks of lipid-rich regions, SMCs, and calcified regions in the atherosclerotic lesions. In addition, IbHE revealed the characteristic distribution of lipids in human atherosclerotic lesions. These data indicate that an IMS-based pathologic approach is of considerable value as a new histopathologic examination. © 2011 Elsevier Ireland Ltd.
  • I-3. the effect of dietary seafood on the brain function: Analysis of brain by imaging mass spectrometry, Nobuhiro Zaima, Nippon Suisan Gakkaishi (Japanese Edition), 77, 260,   2011年06月02日
  • Distribution of phospholipid molecular species in autogenous access grafts for hemodialysis analyzed using imaging mass spectrometry, Hiroki Tanaka, Hiroki Tanaka, Hiroki Tanaka, Nobuhiro Zaima, Naoto Yamamoto, Naoto Yamamoto, Minoru Suzuki, Minoru Suzuki, Yuuki Mano, Yuuki Mano, Hiroyuki Konno, Naoki Unno, Naoki Unno, Mitsutoshi Setou, Analytical and Bioanalytical Chemistry, 400, 1873, 1880,   2011年06月01日
    概要:Arteriovenous fistulae (AVF) using vein grafts are frequently used for vascular access in hemodialysis. When superficial veins are used as autogenous access grafts for hemodialysis, atherosclerotic-like tissue degeneration often causes stenosis and obstruction. Although the differences between the pathology of degeneration in AVF and atherosclerosis (i.e., peripheral artery occlusive disease (PAD)) are known, their underlying molecular mechanisms are not. We determined the characteristic abnormal lipid metabolism of AVF. Oil red O staining clearly showed the accumulation of lipid molecules in AVF and PAD tissues. We found that the staining pattern was different between AVF and PAD tissues. The media and adventitia of AVF and the intima and media of PAD were intensely stained. Quantitative lipid analysis revealed that the amount of PL was significantly increased in AVF and PAD. Next, we performed matrix-assisted laser desorption/ionization imaging mass spectroscopy and determined the characteristic distribution of lysophosphatidylcholine (LPC) and phosphatidylcholine (PC) in AVF. The distribution patterns of LPC (1-acyl 16:0) and PC (diacyl 16:0/20:4) were consistent with the Oil red O staining images, suggesting that metabolisms related to LPC (1-acyl 16:0) and PC (diacyl 16:0/20:4) are altered in AVF. © 2011 Springer-Verlag.
  • Visualization of phosphatidylcholine, lysophosphatidylcholine and sphingomyelin in mouse tongue body by matrix-assisted laser desorption/ ionization imaging mass spectrometry, Hirofumi Enomoto, Yuki Sugiura, Mitsutoshi Setou, Nobuhiro Zaima, Analytical and Bioanalytical Chemistry, 400, 1913, 1921,   2011年06月01日
    概要:The mammalian tongue is one of the most important organs during food uptake because it is helpful for mastication and swallowing. In addition, taste receptors are present on the surface of the tongue. Lipids are the second most abundant biomolecules after water in the tongue. Lipids such as phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM) are considered to play fundamental roles in the mediation of cell signaling. Imaging mass spectrometry (IMS) is powerful tool for determining and visualizing the distribution of lipids across sections of dissected tissue. In this study, we identified and visualized the PC, LPC, and SM species in a mouse tongue body section with matrix-assisted laser desorption/ionization (MALDI)-IMS. The ion image constructed from the peaks revealed that docosahexaenoic acid (DHA)-containing PC, LPC, linoleic acid-containing PC and SM (d18:1/16:0), and oleic acid-containing PC were mainly distributed in muscle, connective tissue, stratified epithelium, and the peripheral nerve, respectively. Furthermore, the distribution of SM (d18:1/16:0) corresponded to the distribution of nerve tissue relating to taste in the stratified epithelium. This study represents the first visualization of PC, LPC and SM localization in the mouse tongue body. © 2011 Springer-Verlag.
  • Abnormal phospholipids distribution in the prefrontal cortex from a patient with schizophrenia revealed by matrix-assisted laser desorption/ionization imaging mass spectrometry, Junya Matsumoto, Yuki Sugiura, Dai Yuki, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Yasuto Kunii, Akira Wada, Qiaohui Yang, Keisuke Nishiura, Hiroyasu Akatsu, Akira Hori, Yoshio Hashizume, Takayuki Yamamoto, Keiko Ikemoto, Mitsutoshi Setou, Shin Ichi Niwa, Analytical and Bioanalytical Chemistry, 400, 1933, 1943,   2011年06月01日
    概要:Schizophrenia is one of the major psychiatric disorders, and lipids have focused on the important roles in this disorder. In fact, lipids related to various functions in the brain. Previous studies have indicated that phospholipids, particularly ones containing polyunsaturated fatty acyl residues, are deficient in postmortem brains from patients with schizophrenia. However, due to the difficulties in handling human postmortem brains, particularly the large size and complex structures of the human brain, there is little agreement regarding the qualitative and quantitative abnormalities of phospholipids in brains from patients with schizophrenia, particularly if corresponding brain regions are not used. In this study, to overcome these problems, we employed matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS), enabling direct microregion analysis of phospholipids in the postmortem brain of a patient with schizophrenia via brain sections prepared on glass slides. With integration of traditional histochemical examination, we could analyze regions of interest in the brain at the micrometric level. We found abnormal phospholipid distributions within internal brain structures, namely, the frontal cortex and occipital cortex. IMS revealed abnormal distributions of phosphatidylcholine molecular species particularly in the cortical layer of frontal cortex region. In addition, the combined use of liquid chromatography/electrospray ionization tandem mass spectrometry strengthened the capability for identification of numerous lipid molecular species. Our results are expected to further elucidate various metabolic processes in the neural system. © 2011 The Author(s).
  • Authenticity assessment of beef origin by principal component analysis of matrix-assisted laser desorption/ionization mass spectrometric data, Nobuhiro Zaima, Naoko Goto-Inoue, Takahiro Hayasaka, Hirofumi Enomoto, Mitsutoshi Setou, Analytical and Bioanalytical Chemistry, 400, 1865, 1871,   2011年06月01日
    概要:It has become necessary to assess the authenticity of beef origin because of concerns regarding human health hazards. In this study, we used a metabolomic approach involving matrix-assisted laser desorption/ionization imaging mass spectrometry to assess the authenticity of beef origin. Highly accurate data were obtained for samples of extracted lipids from beef of different origin; the samples were grouped according to their origin. The analysis of extracted lipids in this study ended within 10 min, suggesting this approach can be used as a simple authenticity assessment before a definitive identification by isotope analysis. © 2011 Springer-Verlag.
  • Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver, Miyamura Norio, Nakamura Takashi, Goto-Inoue Naoko, Zaima Nobuhiro, Hayasaka Takahiro, Yamasaki Tokiwa, Terai Shuji, Sakaida Isao, Setou Mitsutoshi, Nishina Hiroshi, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 408, 1, 120, 125,   2011年04月29日, 査読有り
  • 水産物が脳機能に及ぼす影響 : 質量顕微鏡法を用いた脳の解析, 財満 信宏, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 日本水産學會誌 = Bulletin of the Japanese Society of Scientific Fisheries, 77, 2,   2011年03月15日
  • Selective analysis of lipids by thin-layer chromatography blot matrix-assisted laser desorption/ionization imaging mass spectrometry, Nobuhiro Zaima, Naoko Goto-Inoue, Kohsuke Adachi, Mitsutoshi Setou, Journal of Oleo Science, 60, 93, 98,   2011年01月31日
    概要:Thin-layer chromatography (TLC) is an essential method for food composition analyses such as lipid nutrition analysis. TLC can be used to obtain information about the lipid composition of foods; however, it cannot be used for analyses at the molecular level. Recently we developed a new method that combines matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) with TLCblotting (TLC-Blot-MALDI-IMS). The combination of MALDI-IMS and TLC blotting enabled detailed and sensitive analyses of lipids. In this study, we applied TLC-Blot-MALDI-IMS for analysis of major phospholipids extracted from bluefin tuna. We showed that TLC-Blot-MALDI-IMS analysis could visualize and identify major phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, and sphingomyelin. Copyright ©2011 by Japan Oil Chemists' Society.
  • Using imaging mass spectrometry to accurately diagnose fabry's disease, Kenji Onoue, Kenji Onoue, Nobuhiro Zaima, Yuki Sugiura, Yuki Sugiura, Takuya Isojima, Satoshi Okayama, Manabu Horii, Yasuhiro Akai, Shiro Uemura, Genzou Takemura, Hitoshi Sakuraba, Yasuhiro Sakaguchi, Mitsutoshi Setou, Yoshihiko Saito, Circulation Journal, 75, 221, 223,   2011年01月14日
  • Matrix-assisted laser desorption/ionization imaging mass spectrometry, Nobuhiro Zaima, Takahiro Hayasaka, Naoko Goto-Inoue, Mitsutoshi Setou, International Journal of Molecular Sciences, 11, 5040, 5055,   2010年12月01日
    概要:Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples. © 2010 by the authors.
  • Ionic matrix for enhanced MALDI imaging mass spectrometry for identification of phospholipids in mouse liver and cerebellum tissue sections, Kamlesh Shrivas, Takahiro Hayasaka, Naoko Goto-Inoue, Yuki Sugiura, Nobuhiro Zaima, Mitsutoshi Setou, Analytical Chemistry, 82, 8800, 8806,   2010年11月01日
    概要:The ionic matrix (IM) is considered to be versatile for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the identification of a wide range of biomolecules due to its good solubility for a variety of analytes, formation of homogeneous crystals with analytes, and high vacuum stability. When these advantages are exploited, the performance of IM of α-cyano-4-hydroxycinnamic acid butylamine (CHCAB) and 2,5-dihydroxybenzoic acid butylamine (DHBB) was compared with other matrixes for the identification of phospholipids in standard mixtures and mouse liver tissue sections. The results showed that the IM of CHCAB caused higher signal intensity and allowed the detection of a number phospholipids such as phosphatidylethanolamine (PE) and phosphatidylserine (PS) in addition to detection of phosphatidylcholine (PC) on the surface of the liver tissue sample. The IM of CHCAB was also used to identify the species of lipids present in different layers of cerebellum where the greater numbers of biomolecules were detected as compared to DHB matrix. Further, the feasibility of the proposed method was extended for the analysis of tryptic digested cytochrome c for increased signal intensity and number of peptide sequences in MALDI-MS. Thus, the application of IM to MALDI-MS could be a promising tool for imaging biomolecules in tissue sections in high throughput analyses with high sensitivity. © 2010 American Chemical Society.
  • Imaging mass spectrometry reveals unique lipid distribution in primary varicose veins, H. Tanaka, H. Tanaka, H. Tanaka, N. Zaima, N. Yamamoto, N. Yamamoto, D. Sagara, D. Sagara, M. Suzuki, M. Suzuki, M. Nishiyama, M. Nishiyama, Y. Mano, Y. Mano, M. Sano, M. Sano, T. Hayasaka, N. Goto-Inoue, T. Sasaki, H. Konno, N. Unno, N. Unno, M. Setou, European Journal of Vascular and Endovascular Surgery, 40, 657, 663,   2010年11月01日
    概要:Background: The lipid metabolism of varicose veins (VVs) remains unknown. To elucidate the pathogenesis of VV, we utilized the novel technique of imaging mass spectrometry (IMS). Materials and methods: We obtained VV tissues from 10 limbs of 10 VV patients who underwent great saphenous vein stripping. As control vein samples, we harvested segmental vein tissues from 6 limbs of 6 patients with peripheral artery occlusive disease who underwent infra-inguinal bypass with reversed saphenous vein grafting. To identify the localisation of lipid molecules in the VV tissues, we performed matrix-assisted laser desorption/ionization IMS (MALDI-IMS). We also performed MS/MS analyses to identify the structure of each molecule. Results: We obtained mass spectra directly from control vein tissues and VV tissues and found a unique localisation of lipid molecules in the VV tissues. We localised lysophosphatidylcholine (LPC) (1-acyl 16:0), phosphatidylcholine (PC) (1-acyl 36:4) and sphingomyelin (SM) (d18:1/16:0) at the site of the VV valve. Conclusion: MALDI-IMS revealed the distribution of various lipid molecules in normal veins and VVs both. Accumulation of LPC (1-acyl 16:0), PC (1-acyl 36:4) and SM (d18:1/16:0) in the VV tissues suggested that inflammation associated with abnormal lipid metabolism may contribute to the development of VV. © 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.
  • The detection of glycosphingolipids in brain tissue sections by imaging mass spectrometry using gold nanoparticles, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Yukiyasu Kashiwagi, Mari Yamamoto, Masami Nakamoto, Mitsutoshi Setou, Journal of the American Society for Mass Spectrometry, 21, 1940, 1943,   2010年11月01日
    概要:Glycosphingolipids (GSLs) are amphiphilic molecules consisting of a hydrophilic carbohydrate chain and a hydrophobic ceramide moiety. They appear to be involved primarily in biological processes such as cell proliferation, differentiation, and signaling. To investigate the mechanism of brain function in more detail, a more highly sensitive method that would reveal the GSL distribution in the brain is required. In this report, we describe a simple and efficient method for mapping the distribution and localization of GSLs present in mouse brain sections using nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (IMS). We have developed and tested gold nanoparticles (AuNPs) as a new matrix to maximize the detection of GSLs. A matrix of AuNPs modified with alkylamine was used to detect various GSLs, such as minor molecular species of sulfatides and gangliosides, in mouse brain sections; these GSLs were hardly detected using 2,5-dihydroxybenzoic acid (DHB), which is the conventional matrix for GSLs. We achieved approximately 20 times more sensitive detection of GSLs using AuNPs compared to a DHB matrix. We believe that our new approach using AuNPs in IMS could lead to a new strategy for analyzing basic biological mechanisms and several diseases through the distribution of minor GSLs. © 2010 American Society for Mass Spectrometry.
  • Imaging of lipids in cultured mammalian neurons by matrix assisted laser/desorption ionization and secondary ion mass spectrometry, Hyun Jeong Yang, Hyun Jeong Yang, Yuki Sugiura, Yuki Sugiura, Itsuko Ishizaki, Noriaki Sanada, Koji Ikegami, Nobuhiro Zaima, Kamlesh Shrivas, Mitsutoshi Setou, Surface and Interface Analysis, 42, 1606, 1611,   2010年10月01日
    概要:Imaging mass spectrometry (IMS) provides a novel opportunity for visualization of molecular ion distribution. Currently, there are two major ionization techniques, matrix-assisted laser desorption/ionization (MALDI) and secondary ion mass spectrometry (SIMS) are widely used for imaging of biomolecules in tissue samples. MALDI and SIMS-based IMS have the following features; measurable mass ranges are wide and small, and the spatial resolutions are low and high, respectively. To the best of our knowledge, this is a first report to identify the lipids in cultured mammalian neurons by MALDI-IMS. Further, those neurons were analyzed with SIMS-IMS in order to compare the distribution pattern of lipids and other derived fragments. The parameters which influence the identification of lipids in cultured neurons were optimized in order to get an optimum detection of lipid molecules. The combined spatial data of MALDI and SIMS supported the idea that the signals of small molecules such as phosphatidylcholine head groups and fatty acids (detected in SIMS) are derived from the intact lipids (detected in MALDI-IMS). © 2010 John Wiley & Sons, Ltd.
  • Visualization of biomolecules in the eyestalk of the blue swimming crab, Portunus pelagicus, by imaging mass spectrometry using the atmospheric-pressure mass microscope, Sroyraya Morakot, Goto-Inoue Naoko, Zaima Nobuhiro, Hayasaka Takahiro, Chansela Piyachat, Tanasawet Supita, Shrivas Kamlesh, Sobhon Prasert, Setou Mitsutoshi, SURFACE AND INTERFACE ANALYSIS, 42, 10-11, 1589, 1592,   2010年10月, 査読有り
  • Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry, Hyun Jeong Yang, Hyun Jeong Yang, Itsuko Ishizaki, Noriaki Sanada, Nobuhiro Zaima, Yuki Sugiura, Yuki Sugiura, Ikuko Yao, Koji Ikegami, Mitsutoshi Setou, Medical Molecular Morphology, 43, 158, 164,   2010年09月01日
    概要:Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region. © 2010 The Japanese Society for Clinical Molecular Morphology.
  • Application of imaging mass spectrometry for the analysis of Oryza sativa rice, Nobuhiro Zaima, Naoko Goto-Inoue, Takahiro Hayasaka, Mitsutoshi Setou, Rapid Communications in Mass Spectrometry, 24, 2723, 2729,   2010年09月01日
    概要:Rice is one of the most important food crops in the world and new varieties have been bred for specific purposes, such as the development of drought-resistance, or the enrichment of functional food factors. The localization and composition of metabolites in such new varieties must be investigated because all artificial interventions are expected to change the metabolites of rice. Imaging mass spectrometry using matrix-assisted laser desorption/ionization (MALDI-IMS) is a suitable tool for investigating the localization and composition of metabolites; however, suitable methodologies for the MALDI-IMS analysis of rice have not yet been established. In this study, we optimized the methods for analyzing rice grains by MALDI-IMS using adhesive film and found the characteristic distribution of metabolites in rice. Lysophosphatidylcholine (LPC) was localized in the endosperm. Phosphatidylcholine (PC), γ-oryzanol and phytic acid were localized in the bran (germ and seed coat), and α-tocopherol was distributed in the germ (especially in the scutellum). In addition, MALDI-IMS revealed the LPC and PC composition of the rice samples. The LPC composition, LPC (1-acyl 16:0), LPC (1-acyl 18:2), LPC (1-acyl 18:1) and LPC (1-acyl 18:0), was 59.4±4.5%, 19.6±2.5%, 14.2±4.5% and 6.8±1.4%. The PC composition, PC (diacyl 16:0/18:2), PC (diacyl 16:0/18:1), PC (diacyl 18:1/18:3), PC (diacyl 18:1/18:2) and PC (diacyl 18:1/18:2), was 19.6±1.0%, 21.0±1.0%, 15.0±1.4%, 26.7±0.7% and 17.8±1.9%. This approach can be applied to the assessment of metabolites not only in rice, but also in other foods for which the preparation of sections is a challenging task. © 2010 John Wiley & Sons, Ltd.
  • SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals, Sayaka Akieda-Asai, Sayaka Akieda-Asai, Nobuhiro Zaima, Nobuhiro Zaima, Koji Ikegami, Koji Ikegami, Tomoaki Kahyo, Tomoaki Kahyo, Ikuko Yao, Ikuko Yao, Takahiro Hatanaka, Shun ichiro Iemura, Rika Sugiyama, Takeaki Yokozeki, Yoshinobu Eishi, Morio Koike, Kyoji Ikeda, Takuya Chiba, Haruyoshi Yamaza, Haruyoshi Yamaza, Isao Shimokawa, Si Young Song, Akira Matsuno, Akiko Mizutani, Motoji Sawabe, Moses V. Chao, Masashi Tanaka, Yasunori Kanaho, Tohru Natsume, Haruhiko Sugimura, Yukari Date, Michael W. Mcburney, Leonard Guarente, Mitsutoshi Setou, Mitsutoshi Setou, PLoS ONE, 5,   2010年08月16日
    概要:Background: SIRT1, a NAD-dependent deacetylase, has diverse roles in a variety of organs such as regulation of endocrine function and metabolism. However, it remains to be addressed how it regulates hormone release there. Methodology/Principal Findings: Here, we report that SIRT1 is abundantly expressed in pituitary thyrotropes and regulates thyroid hormone secretion. Manipulation of SIRT1 level revealed that SIRT1 positively regulated the exocytosis of TSH-containing granules. Using LC/MS-based interactomics, phosphatidylinositol-4-phosphate 5-kinase (PIP5K)y was identified as a SIRT1 binding partner and deacetylation substrate. SIRT1 deacetylated two specific lysine residues (K265/K268) in PIP5Ky and enhanced PIP5Ky enzyme activity. SIRT1-mediated TSH secretion was abolished by PIP5Ky knockdown. SIRT1 knockdown decreased the levels of deacetylated PIP5Ky, PI(4,5)P 2 , and reduced the secretion of TSH from pituitary cells. These results were also observed in SIRT1-knockout mice. Conclusions/Significance: Our findings indicated that the control of TSH release by the SIRT1-PIP5Kγ pathway is important for regulating the metabolism of the whole body. © 2010 Akieda-Asai et al.
  • Dominant-negative effects of a novel mutation in the filamin myopathy, S. Kono, T. Nishio, Y. Takahashi, Y. Takahashi, N. Goto-Inoue, M. Kinoshita, N. Zaima, H. Suzuki, A. Fukutoku-Otsuji, A. Fukutoku-Otsuji, M. Setou, H. Miyajima, Neurology, 75, 547, 554,   2010年08月10日
    概要:Background: Filamin myopathy is associated with mutations in the filamin C gene (FLNC) and is a myofibrillar myopathy characterized by focal myofibrillar destruction and cytoplasmic aggregates containing several Z-disk-related proteins. Methods: This study investigated 6 Japanese patients with dominantly in herited myofibrillar myopathy manifested by adult-onset, slow and progressive muscle weakness and atrophy in the distal extremities. Results: The abundantly expressed proteins in the affected muscles were identified as filamin C by nano liquid chromatography-tandem mass spectrometry. A genetic analysis of FLNC identified a heterozygous c.8107delG mutation that was localized to the dimerization domain of filamin C. A biochemical crosslinking analysis of bacterially expressed recombinant wild-type and mutant filamin C fragments demonstrated that the mutant monomer disturbed the proper dimerization of the wild-type filamin dimer, resulting in formation of a heterotrimer with the wild-type filamin dimer. The expression study in C2C12 myoblasts showed that the mutant filamin fragments formed cytoplasmic aggregates with endogenous wild-type filamin C. Conclusions: This study provides evidence for the dominant-negative effects of the FLNC mutation. These effects may be mutation-specific and likely result in the variation in the clinical phenotypes seen in patients with filamin myopathy. © 2010 by AAN Enterprises, Inc. All rights reserved.
  • Imaging mass spectrometry with silver nanoparticles reveals the distribution of fatty acids in mouse retinal sections, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Kamlesh Shrivas, Kamlesh Shrivas, Yukiyasu Kashiwagi, Mari Yamamoto, Masami Nakamoto, Mitsutoshi Setoua, Journal of the American Society for Mass Spectrometry, 21, 1446, 1454,   2010年08月01日
    概要:A new approach to the visualization of fatty acids in mouse liver and retinal samples has been developed using silver nanoparticles (AgNPs) in nanoparticle-assisted laser desorption/ ionization imaging mass spectrometry (nano-PALDI-IMS) in negative ion mode. So far, IMS analysis has concentrated on main cell components, such as cell membrane phospholipids and cytoskeletal peptides. AgNPs modified with alkylcarboxylate and alkylamine were used for nano-PALDI-IMS to identify fatty acids, such as stearic, oleic, linoleic, arachidonic, and eicosapentaenoic acids, as well as palmitic acid, in mouse liver sections; these fatty acids are not detected using 2,5-dihydroxybenzoic acid (DHB) as a matrix. The limit of detection for the determination of palmitic acid was 50 pmol using nano-PALDI-IMS. The nano-PALDI-IMS method is successfully applied to the reconstruction of the ion images of fatty acids in mouse liver sections. We verified the detection of fatty acids in liver tissue sections of mice by analyzing standard lipid samples, which showed that fatty acids were from free fatty acids and dissociated fatty acids from lipids when irradiated with a laser. Additionally, we applied the proposed method to the identification of fatty acids in mouse retinal tissue sections, which enabled us to learn the six-zonal distribution of fatty acids in different layers of the retina. We believe that the current approach using AgNPs in nano-PALDI-IMS could lead to a new strategy to analyze basic biological mechanisms and several diseases through the distribution of fatty acids. © 2010 American Society for Mass Spectrometry.
  • Visualization of spatial distribution of γ-aminobutyric acid in eggplant (Solanum melongena) by matrix-assisted laser desorption/ionization imaging mass spectrometry, Naoko Goto-Inoue, Mitsutoshi Setou, Nobuhiro Zaima, Analytical Sciences, 26, 821, 825,   2010年07月01日
    概要:We applied imaging mass spectrometry (IMS) to determine the spatial distribution of γ-aminobutyric acid (GABA). We found that GABA had a specific localization in seeds. We also visualized various biomolecules as well as GABA with higher spatial resolution than in the previous report. Our work suggests that IMS might be a powerful tool for exploring functional food factors, investigating the specific distribution of nutrients in unused natural resources, and evaluating the quality of functional foods. 2010 © The Japan Society for Analytical Chemistry.
  • 質量顕微鏡法の開発と応用, 尾上 健児, 財満 信宏, 斎藤 能彦, 内分泌・糖尿病・代謝内科, 内分泌・糖尿病・代謝内科, 30, 4, 359, 367,   2010年04月
  • Developments and applications of mass microscopy, Mitsutoshi Setou, Kamlesh Shrivas, Morakot Sroyraya, Morakot Sroyraya, Hyunjeong Yang, Yuki Sugiura, Junji Moribe, Akira Kondo, Koji Tsutsumi, Yoshishige Kimura, Nobuya Kurabe, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Koji Ikegami, Prasert Sobhon, Yoshiyuki Konishi, Medical Molecular Morphology, 43, 1, 5,   2010年03月01日
    概要:We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on some of the recent developments in clinical applications to show how mass microscopy can be employed to assess medical molecular morphology. © The Japanese Society for Clinical Molecular Morphology 2010.
  • Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray, Yoshifumi Morita, Yoshifumi Morita, Koji Ikegami, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Hiroki Tanaka, Takashi Uehara, Tomohiko Setoguchi, Takanori Sakaguchi, Hisashi Igarashi, Haruhiko Sugimura, Mitsutoshi Setou, Hiroyuki Konno, Cancer Science, 101, 267, 273,   2010年01月01日
    概要:The popularity of imaging mass spectrometry (IMS) of tissue samples, which enables the direct scanning of tissue sections within a short time-period, has been considerably increasing in cancer proteomics. Most pathological specimens stored in medical institutes are formalin-fixed; thus, they had been regarded to be unsuitable for proteomic analyses, including IMS, until recently. Here, we report an easy-to-use screening method that enables the analysis of multiple samples in one experiment without extractions and purifications of proteins. We scanned, with an IMS technique, a tissue microarray (TMA) of formalin-fixed paraffin-embedded (FFPE) specimens. We detected a large amount of signals from trypsin-treated FFPE-TMA samples of gastric carcinoma tissues of different histological types. Of the signals detected, 54 were classified as signals specific to cancer with statistically significant differences between adenocarcinomas and normal tissues. We detected a total of 14 of the 54 signals as histological type-specific with the support of statistical analyses. Tandem MS revealed that a signal specific to poorly differentiated cancer tissue corresponded to histone H4. Finally, we verified the IMS-based finding by immunohistochemical analysis of more than 300 specimens spotted on TMAs; the immunoreactivity of histone H4 was remarkably strong in poorly differentiated cancer tissues. Thus, the application of IMS to FFPE-TMA can enable high-throughput analysis in cancer proteomics to aid in the understanding of molecular mechanisms underlying carcinogenesis, invasiveness, metastasis, and prognosis. Further, results obtained from the IMS of FFPE-TMA can be readily confirmed by commonly used immunohistochemical analyses. © 2009 Japanese Cancer Association.
  • Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry, Yuki Sugiura, Yuki Sugiura, Yuki Sugiura, Yoshiyuki Konishi, Yoshiyuki Konishi, Nobuhiro Zaima, Nobuhiro Zaima, Shigeki Kajihara, Hiroki Nakanishi, Hiroki Nakanishi, Ryo Taguchi, Ryo Taguchi, Mitsutoshi Setou, Mitsutoshi Setou, Journal of Lipid Research, 50, 1776, 1788,   2009年11月02日
    概要:Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions. Copyright © 2009 by the American Society for Biochemistry and Molecular Biology, Inc.
  • Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope, Takahiro Harada, Akiko Yuba-Kubo, Yuki Sugiura, Yuki Sugiura, Nobuhiro Zaima, Takahiro Hayasaka, Naoko Goto-Inoue, Masatoshi Wakui, Makoto Suematsu, Kengo Takeshita, Kiyoshi Ogawa, Yoshikazu Yoshida, Mitsutoshi Setou, Analytical Chemistry, 81, 9153, 9157,   2009年11月01日
    概要:We have developed a mass microscope (mass spectrometry imager with spatial resolution higher than the naked eye) equipped with an atmospheric pressure ion-source chamber for laser desorption/ionization (AP-LDI) and a quadrupole ion trap time-of-flight (QIT-TOF) analyzer. The optical microscope combined with the mass spectrometer permitted us to precisely determine the relevant tissue region prior to performing imaging mass spectrometry (IMS). An ultraviolet laser tightly focused with a triplet lens was used to achieve high spatial resolution. An atmospheric pressure ion-source chamber enables us to analyze fresh samples with minimal loss of intrinsic water or volatile compounds. Mass-microscopic AP-LDI imaging of freshly cut ginger rhizome sections revealed that 6-gingerol ([M + K] + at m/z 333.15, positive mode; [M - H] - at m/z 293.17, negative mode) and the monoterpene ([M + K] + at m/z 191.09), which are the compounds related to pungency and flavor, respectively, were localized in oil drop-containing organelles. AP-LDI-tandem MS/MS analyses were applied to compare authentic signals from freshly cut ginger directly with the standard reagent. Thus, our atmosphereimaging mass spectrometer enabled us to monitor a quality of plants at the organelle level. © 2009 American Chemical Society.
  • Organ-specific distributions of lysophosphatidylcholine and triacylglycerol in mouse embryo, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Yoshishige Kimura, Mitsutoshi Setou, Lipids, 44, 837, 848,   2009年09月01日
    概要:Imaging mass spectrometry (IMS) has been developed as a method for determining and visualizing the distribution of proteins and lipids across sections of dissected tissue. Although lipids play an important role in mammal development, their detailed distributions have not been analyzed by conventional methods. In this study, we tried to determine and visualize lysophosphatidylcholine (LysoPtdCho) and triacylglycerol (TAG) in a mouse embryo by matrix-assisted laser desorption/ionization (MALDI) hybrid quadrupole time-of-flight (TOF) mass spectrometer. Many peaks were detected from a raster scan of the whole embryonic sections. The peaks at m/z 496.33, 524.36, 879.72, 881.74, and 921.74 were identified by MS/MS analyses as [LysoPtdCho (16:0) + H] + , [LysoPtdCho (18:0) + H] + , [TAG (16:0/18:2/18:1) + Na] + , [TAG (16:0/18:1/18:1) + Na] + , and [TAG (16:0/20:3/18:1) + K] + , respectively. The ion images constructed from the peaks revealed that LysoPtdCho were distributed throughout the body and TAGs were distributed around the brown adipose tissue and in the liver at embryo day 17.5. Thus, IMS system based on MALDI hybrid quadrupole TOF MS revealed the distribution of LysoPtdCho and, more importantly, the organ-specific distribution of TAGs in the embryonic stages of mammals for the first time. We can conclude that this technique enables us to analyze the roles of various lipids during embryogenesis and gives insight for lipid research. © 2009 AOCS.
  • The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry, Naoko Goto-Inoue, Takahiro Hayasaka, Nobuhiro Zaima, Mitsutoshi Setou, Glycobiology, 19, 950, 957,   2009年08月20日
    概要:More than 90% of the glycolipid in mammalian testis consists of a unique sulfated glyceroglycolipid called seminolipid. The galactosylation of the molecule is catalyzed by UDP-galactose:ceramide galactosyltransferase (CGT). Disruption of the CGT gene in mice results in male infertility due to the arrest of spermatogenesis, indicating that seminolipid plays an important role in reproductive function. Seminolipid molecules can be assigned to different molecular species based on the fatty acid composition. In this report, we investigated the localizations of the molecular species of seminolipid by imaging mass spectrometry and demonstrated that major molecule (C16:0-alkyl-C16:0-acyl) was expressed throughout the tubules: some (C16:0-alkyl-C14:0-acyl and C14:0-alkyl-C16:0-acyl) were predominantly expressed in spermatocytes and the other (C17:0-alkyl-C16:0-acyl) was specifically expressed in spermatids and spermatozoa. This is the first report to show the cell-specific localization of each molecular species of seminolipid during testicular maturation. © The Author 2009. Published by Oxford University Press. All rights reserved.
  • Imaging of metabolites by MALDI mass spectrometry, Nobuhiro Zaima, Takahiro Hayasaka, Naoko Goto-Inoue, Mitsutoshi Setou, Journal of Oleo Science, 58, 415, 419,   2009年07月08日
    概要:Visualization of endogenous molecules in small experimental animals is very important in order to investigate the localization of specific molecules. Conventional imaging techniques are not suitable for simultaneous visualization of endogenous molecules. We recently developed a new imaging method-imaging mass spectrometry (IMS) using matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF). In this study, we applied IMS using MALDI-QIT-TOF to visualize the metabolites in medaka (Oryzias latipes) sections and determined the spatial distribution of metabolites including lipids. © 2009 by Japan Oil Chemists' Society.
  • Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry, Hiroshi Ageta, Sayaka Asai, Yuki Sugiura, Yuki Sugiura, Yuki Sugiura, Naoko Goto-Inoue, Nobuhiro Zaima, Nobuhiro Zaima, Mitsutoshi Setou, Mitsutoshi Setou, Medical Molecular Morphology, 42, 16, 23,   2009年03月01日
    概要:Lipids are major structural component of the brain and play key roles in signaling functions in the central nervous system (CNS), such as the hippocampus. In particular, sulfatide is an abundant glycosphingolipid component of both the central and the peripheral nervous system and is an essential lipid component of myelin membranes. Lack of sulfatide is observed in myelin deformation and neurological deficits. Previous studies with antisulfatide antibody have investigated distribution of sulfatide expression in neurons; however, this method cannot distinguish the differences of sulfatide lipid species raised by difference of carbon-chain length in the ceramide por tion in addition to the differences of sulfatide and seminolipid. In this study, we solved the problem by our recently developed nanoparticle-assisted laser desorption/ionization (nano-PALDI)-based imaging mass spectrometry (IMS). We revealed that the level of sulfatide in the middle molecular layer was significantly higher than that in granule cell layers and the inner molecular layer in the dentate gyrus of rat hippocampus. © 2009 The Japanese Society for Clinical Molecular Morphology.
  • Principal Component Analysis of Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Data Related to Metabolites of Fatty Liver, Nobuhiro Zaima, Nobuhiro Zaima, Yumiko Matsuyama, Mitsutoshi Setou, Mitsutoshi Setou, Journal of Oleo Science, 58, 267, 273,   2009年01月01日
    概要:Non-alcoholic steatohepatitis (NASH) is a common liver disease. NASH is characterized by fatty liver, along with inflammation. Most people with NASH are not aware of their condition, even though NASH can lead to hepatic cirrhosis. Several approaches have been tested to clarify the pathology of NASH. However, the mechanism of onset of NASH was not well-defined. In this study, a supervised multivariate analysis (principal component analysis) approach using direct matrix-assisted laser desorption/ionization mass spectrometry (dMALDI-MS) was applied to the analysis of metabolites in starvation-induced fatty liver tissue sections. This approach does not require complex pretreatments. We investigated the characteristic dynamics of metabolites in fatty liver. This approach can be applied to the analysis of human biopsy specimens of fatty liver in future studies. © 2009, Japan Oil Chemists' Society. All rights reserved.
  • Triglyceride deposit cardiomyovasculopathy, Ken Ichi Hirano, Yoshihiko Ikeda, Nobuhiro Zaima, Yasuhiko Sakata, Goro Matsumiya, New England Journal of Medicine, 359, 2396, 2398,   2008年11月27日
  • Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF)-based imaging mass spectrometry reveals a layered distribution of phospholipid molecular species in the mouse retina, Hayasaka Takahiro, Goto-Inoue Naoko, Sugiura Yuki, Zaima Nobuhiro, Nakanish Hiroki, Ohishi Kentaro, Nakanish Setsuko, Naito Takayuki, Taguchi Ryo, Setou Mitsutoshi, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 22, 21, 3415, 3426,   2008年11月, 査読有り
  • Effects of trans and conjugated LC N-3 polyunsaturated fatty acids on lipid composition and abdominal fat weight in rats, T. Okada, R. Noguchi, M. Hosokawa, K. Fukunaga, T. Nishiyama, N. Zaima, T. Hirata, K. Miyashita, Journal of Food Science, 73,   2008年10月01日
    概要:Trans and conjugated fatty acids may exhibit either beneficial or detrimental bioactive effects depending on their metabolic properties. This study was conducted to elucidate if isomerization and conjugation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) demonstrate more favorable bioactivity on lipid metabolism compared to unmodified EPA and DHA. The effects of dietary intake of trans and conjugated forms of EPA and DHA on lipid metabolism were evaluated in animal trials and compared to a control group fed soybean oil. None of the experimental diets showed significant differences from the control in terms of body weight; however, the white adipose tissue weight of rodents fed trans DHA, conjugated EPA (CEPA), and conjugated DHA (CDHA) was significantly lower than the control. Triacylglycerol levels in plasma were significantly decreased in groups fed trans DHA (17.2 mg/dL) and CDHA (31.9 mg/dL) relative to the control (51.3 mg/dL). The total cholesterol concentrations were significantly lower than the control (68.0 mg/dL) in all experimental groups (47.3 to 53.7 mg/dL) except CEPA (58.3 mg/dL). Fatty acid compositions of lipids extracted from rodent livers were influenced by the dietary fatty acid profiles, with all groups showing higher concentrations of stearic acid and lower levels of linoleic acid compared to the control. Rodents fed trans DHA did not have detectable levels of these fatty acid isomers in their livers, suggesting either quick metabolism or a difficulty with bio-absorption. © 2008 Institute of Food Technologists®.
  • Mass imaging and identification of biomolecules with MALDI-QIT-TOF-based system, Shuichi Shimma, Shuichi Shimma, Shuichi Shimma, Yuki Sugiura, Takahiro Hayasaka, Nobuhiro Zaima, Mineo Matsumoto, Mitsutoshi Setou, Mitsutoshi Setou, Mitsutoshi Setou, Analytical Chemistry, 80, 878, 885,   2008年02月01日
    概要:Imaging mass spectrometry is becoming a popular visualization technique in the medical and biological sciences. For its continued development, the ability to both visualize and identify molecules directiy on the tissue surface using tandem mass spectrometry (MS n ) is essential. We established an imaging system based on a matrix-assisted laser/desorption ionization quadrupole ion trap time-of-flight type instrument (AXIMA-QIT, Shimadzu, Kyoto, Japan), which was compatible with both imaging and highly sensitive MS n . In this paper, we present the operating conditions of the AXIMA-QIT as an imaging instrument and introduce the data converter we developed that is available free of charge. The converted data can be applied to Biomap, the commonly used visualization software. For the feasibility experiments, we demonstrated the visualization of phospholipids, glycolipid, and tryptic-digested proteins in the mouse cerebellum. The visualized lipids were successfully identified by MS n directiy on the tissue surface, with a strong ability to isolate precursor ions. In the analysis of trypt ic-digested proteins, we compared the product ion spectra between AXIMA-QIT and a tandem TOF-type instrument The results confirmed that AXIMA-QIT can provide a high quality of product ion spectra even on the tissue surface. © 2008 American Chemical Society.
  • Effects of reactive radicals and heat on trans-isomerization of eicosapentaenoic acid, Nobuhiro Zaima, Tatsuya Sugawara, Yuki Otsubo, Takashi Hirata, Fisheries Science, 73, 897, 901,   2007年08月01日
    概要:Trans geometric isomers of eicosapentaenoic acid (TEPA) have been found as minor components in human platelets. However, there is little information on the mechanism of trans-isomerization of eicosapentaenoic acid (EPA) in vitro and in vivo. The effects of reactive radicals and heat on trans-isomerization of EPA were examined. Trans-isomerization occurred when EPA ethyl ester reacted with nitrogen dioxide radical (NO 2 ) but not with 2,2′-azobis(2,4- dimethylvaleronitrile). TEPA was also produced from EPA ethyl ester heated at 200°C for 60 h. No TEPA, however, was detected in sardines Sardinops melanostictus after boiling, roasting, or microwave heating. These results suggest that EPA is trans-isomerized by NO 2 in vivo while trans-isomerization of EPA does not occur during conventional cooking. © 2007 Japanese Society of Fisheries Science.
  • Isolation of sphingoid bases of sea cucumber cerebrosides and their cytotoxicity against human colon cancer cells, Tatsuya Sugawara, Nobuhiro Zaima, Akiyo Yamamoto, Shota Sakai, Ryoko Noguchi, Ryoko Noguchi, Takashi Hirata, Bioscience, Biotechnology and Biochemistry, 70, 2906, 2912,   2006年12月01日
    概要:Sea cucumber is a health-beneficial food, and contains a variety of physiologically active substances including glycosphingolipids. We show here the sphingoid base composition of cerebrosides prepared from sea cucumber and the cytotoxicity against human colon cancer cell lines. The composition of sphingoid bases prepared from sea cucumber was different from that of mammals, and the major constituents estimated from mass spectra had a branched C17-19 alkyl chain with 1-3 double bonds. The viability of DLD-1, WiDr and Caco-2 cells treated with sea cucumber sphingoid bases was reduced in a dose-dependent manner and was similar to that of cells treated with sphingosine. The sphingoid bases induced such a morphological change as condensed chromatin fragments and increased the caspase-3 activity, indicating that the sphingoid bases reduced the cell viability by causing apoptosis in these cells. Sphingolipids of sea cucumber might therefore serve as bioactive dietary components to suppress colon cancer.
  • Trans geometric isomers of EPA decrease LXRα-induced cellular triacylglycerol via suppression of SREBP-1c and PGC-1β, Nobuhiro Zaima, Tatsuya Sugawara, Dai Goto, Takashi Hirata, Journal of Lipid Research, 47, 2712, 2717,   2006年12月01日
    概要:Dietary mono- or di-trans fatty acids with chain lengths of 18-22 increase the risk of cardiovascular diseases because they increase LDL cholesterol and decrease HDL cholesterol in the plasma. However, the effects of trans isomers of PUFAs on lipid metabolism remain unknown. Dietary PUFAs, especially eicosapentaenoic acid (EPA) in marine oils, improve serum lipid profiles by suppressing liver X receptor a (LXRα) activity in the liver. In this study, we compared the effects of trans geometric isomers of eicosapentaenoic acid (TEPA) on triacylglycerol synthesis induced by a synthetic LXRα agonist (T0901317) with the effects of EPA in HepG2 cells. TEPA significantly decreased the amount of cellular triacylglycerol and the expression of mRNAs encoding fatty acid synthase, stearoyl-CoA desaturase-1, and glycerol-3-phosphate acyltransferase induced by T0901317 compared with EPA. However, there was no significant difference between the suppressive effect of TEPA or EPA on the expression of sterol-regulatory element binding protein-1c (SREBP-1c) induced by T0901317. We found that TEPA, but not EPA, decreased the mRNA expression of peroxisome proliferator-activated receptor g coactivator 1β (PGC-1β), which is a coactivator of both LXRα and SREBP-1. These results suggest that the hypolipidemic effect of TEPA can be attributed to a decrease not only in SREBP-1 but also in PGC-1β expression. Copyright ©2006 by the American Society for Biochemistry and Molecular Biology, Inc.
  • ケナフのハイドロトロピックパルプ化と酵素漂白(<特集>木質材料), 巽 大輔, 財満 信宏, 松本 孝芳, 杉山 英彦, 高橋 治雄, 材料, 材料, 55, 4, 363, 366,   2006年04月15日
    概要:Kenaf is a fast-growing plant and is noticed as a nonwood fiber resource. There will be significance in the kenaf utilization when the unique characteristics of kenaf will be effective for certain purposes. In this study, pulping and bleaching of kenaf were carried out to examine the usability of kenaf as a fibrous material. We examined a hydrotropic pulping and an enzymatic bleaching, considering the environmental feasibility. Sodium m-xylene sulfonate solution (NaXS) and sodium p-toluene sulfonate solution (NaTS) were used as the hydrotropic regents. The characteristic peaks for lignin were detected by means of UV, ^<13>C-NMR, and FT IR measurements of the solution after the hydrotropic cooking. The solution after the NaTS cooking produced precipitation when it was stand at room temperature, although the NaXS was stable. This shows that NaXS has higher power of dissolution of lignin. The molecular orbital calculation implied that this difference was originated from the micelle forming ability of the regents. The enzymatic bleaching by manganese peroxidase (MnP) was applied for the obtained pulp. The brightness was increased in 17.4% and the Kappa number was lowered in 9.0 for the bast fibers by the biobleaching.
  • Hydrotropic pulping and biobleaching of kenaf, Daisuke Tatsumi, Nobuhiro Zaima, Takayoshi Matsumoto, Hidehiko Sugiyama, Haruo Takahashi, Zairyo/Journal of the Society of Materials Science, Japan, 55, 363, 366,   2006年04月01日
    概要:Kenaf is a fast-growing plant and is noticed as a nonwood fiber resource. There will be significance in the kenaf utilization when the unique characteristics of kenaf will be effective for certain purposes. In this study, pulping and bleaching of kenaf were carried out to examine the usability of kenaf as a fibrous material. We examined a hydrotropic pulping and an enzymatic bleaching, considering the environmental feasibility. Sodium m-xylene sulfonate solution (NaXS) and sodium p-toluene sulfonate solution (NaTS) were used as the hydrotropic regents. The characteristic peaks for lignin were detected by means of UV, 13 C-NMR, and FT-IR measurements of the solution after the hydrotropic cooking. The solution after the NaTS cooking produced precipitation when it was stand at room temperature, although the NaXS was stable. This shows that NaXS has higher power of dissolution of lignin. The molecular orbital calculation implied that this difference was originated from the micelle forming ability of the regents. The enzymatic bleaching by manganese peroxidase (MnP) was applied for the obtained pulp. The brightness was increased in 17.4% and the Kappa number was lowered in 9.0 for the bast fibers by the biobleaching.
  • Characterization of Trans Eicosapentaenoic Acid Isomers: Oxidative Stability and Anti-Inflammatory Activity, Nobuhiro Zaima, Tatsuya Sugawara, Hirofumi Arai, Kazuyuki Nakamura, Mikako Takasugi, Kenji Fukunaga, Ryoko Noguchi, Takashi Hirata, Journal of Oleo Science, 54, 505, 512,   2005年01月01日
    概要:Although trans geometric isomers of unsaturated fatty acids are formed during heat treatment, little is known about the physicochemical properties and the bioactivities of trans eicosapentaenoic acid (TEPA). We examined the oxidative stability and the anti-inflammatory activity of TEPA prepared using p-toluenesulfinic acid. TEPA was more stable to oxidation induced by radical generators than eicosapentaenoic acid (EPA) in organic and in aqueous solutions, and was less suitable as a substrate for 15-lipoxygenase oxidation than was EPA. These results suggest that the trans conformation may be a more stable structure to oxidation. In addition, the release of leukotriene B 4 (LTB 4 ) from Ca-ionophore-stimulated rat peritoneal exudate cells (PEC) was not affected by treatment with TEPA, and TEPA was taken up into PEC at lower levels compared to EPA. These results suggest that dietary TEPA has different physiological effects than EPA. © 2005, Japan Oil Chemists' Society. All rights reserved.

書籍等出版物

  • アンチエイジングをめざした水産物の利用 (水産学シリーズ), 平田 孝;菅原 達也;又平 芳春;酒井 祥太;都築 毅;宮澤 陽夫;橋本 道男;財満 信宏;齋藤 洋昭;眞岡 孝至;水田 尚志, 恒星社厚生閣,   2011年10月06日, 4769912595
  • Imaging Mass Spectrometry, 財満信宏, 共著, Springer,   2010年01月
  • 質量顕微鏡法, 財満信宏, 共著, シュプリンガージャパン株式会社,   2008年11月
  • 栄養素でわかる食品と健康=WEB連動テキスト, 財満信宏, 分担執筆, 培風館,   2016年04月
  • Encyclopedia of Analytical Chemistry., 財満信宏, 共著, John Wiley & Sons, Ltd,   2015年09月
  • Pharmacology, 財満信宏, 共著

講演・口頭発表等

  • 大豆サポニンアグリコン(ソヤサポゲノール)の脂肪細胞における脂肪蓄積への影響, 髙田祐一,加茂修一、岩本和子、川畑葉月、財満信宏,佐藤俊郎、森山達哉, 第1回中性脂肪学会学術集会,   2017年09月09日
  • 脂肪分の過剰摂取がAAAに与える影響, 橋本佳祐、久後裕菜、宮本智絵、財満信宏、森山達哉, 第1回中性脂肪学会学術集会,   2017年09月09日
  • 高シュークロース食の摂取が腹部大動脈瘤の進展・破裂と血管壁に出現する脂肪細胞へ及ぼす影響の病理的評価, 宮本 智絵、橋本 佳祐、久後 裕菜、財満 信宏、森山 達哉, 第1回中性脂肪学会学術集会,   2017年09月09日
  • 腹部大動脈瘤血管壁における脂肪細胞出現機構の解明, 久後 裕菜、財満 信宏、森山 達哉, 第1回中性脂肪学会学術集会,   2017年09月09日
  • 卵巣摘出(OVX)マウスにおける脂質代謝関連因子に及ぼすTaheebo (Tabebuia avellanedae Lorentz ex Griseb) 抽出物の影響の評価, 岩本和子・福田陽一・土岐倉千智・野田真祐子・山本彩実・山本美里・山下光明・財満信宏・飯田彰・森山達哉, 第1回中性脂肪学会学術集会,   2017年09月09日
  • 腹部大動脈瘤破裂と TG の関係, 財満信宏, 第1回 中性脂肪学会学術集会 ,   2017年09月09日, 招待有り
  • 食な新たなリスク:花粉症に関連する新規な食物アレルギー, 花房佳世, 植田啄也, 村上浩規, 矢野えりか, 財満信宏, 森山達哉, アグリバイオ,   2017年08月31日
  • 中性脂肪蓄積心筋血管症における冠動脈硬化の病理学的特徴と画像との対比, 池田善彦, 平野賢一, 財満信宏, 東将浩, 日本動脈硬化学会総会・学術集会プログラム・抄録集(Web),   2017年06月25日
  • 腹部大動脈瘤の破裂機構の解明と破裂予防法の検討, 財満信宏, 久後裕菜, 田中宏樹, 柳本賢一, 佐々木健, 浦野哲盟, 海野直樹, 森山達哉, 脂質生化学研究,   2017年06月01日
  • 血管壁に異常出現した脂肪細胞が腹部大動脈瘤破裂を誘導する, 久後裕菜、財満信宏、田中宏樹、毛利陽平、柳本賢一、佐々木健、浦野哲盟、海野直樹、森山達哉, 第71回日本栄養・食糧学会,   2017年05月21日
  • 高シュークロース食摂取による腹部大動脈瘤の進展・破裂への影響評価, 宮本智絵、橋本佳祐、久後裕菜、財満信宏、森山達哉, 第71回日本栄養・食糧学会,   2017年05月21日
  • エラグ酸がアディポサイトカイン類とリポタンパク質の分泌に及ぼす効果, 家田彩菜、直江美咲、奥野悠子、岩本和子、財満信宏、森山達哉, 第71回日本栄養食糧学会,   2017年05月21日
  • 質量分析イメージングによるアントシアニン分子種のマウス体内動態解析, 山田周平、財満信宏、荘厳哲哉、森山達哉, 第71回日本栄養・食糧学会,   2017年05月21日
  • 卵巣摘出 (OVX) マウスにおけるTaheebo (Tabebuia avellanedae Lorentz ex Griseb) 抽出物の抗肥満効果とその作用物質の同定, 岩本和子、福田陽一、土岐倉千智、野田真祐子、山本彩実、山本美里、山下光明、財満信宏、飯田彰、森山達哉, 第71回日本栄養・食糧学会,   2017年05月20日
  • マウスモデル系を用いた卵白タンパク質の経皮感作条件の検討, 小川昂志、高葢秋穂、村上浩規、岩本和子、矢野えりか、財満信宏 、森山達哉, 第71回日本栄養・食糧学会,   2017年05月20日
  • マウスモデル系を用いたソバタンパク質の経皮感作に関する研究, 高葢秋穂、村上浩規、小川昂志、岩本和子、矢野えりか、財満信宏、森山達哉, 第71回日本栄養・食糧学会,   2017年05月20日
  • 大豆サポニンアグリコン(サポゲノール)の脂肪細胞機能に及ぼす影響, 髙田祐一,加茂修一、岩本和子、川畑葉月、財満信宏,佐藤俊郎、森山達哉, 第71回日本栄養・食糧学会,   2017年05月20日
  • マウスを用いた卵白タンパク質の経皮感作モデル系の確立と高度化, 小川昂志、高葢秋穂、村上浩規、岩本和子、矢野えりか、財満信宏、森山達哉, 日本農芸化学会 2017年度大会,   2017年03月18日
  • 腹部大動脈瘤の進展・破裂に及ぼす高脂肪食の影響評価, 橋本佳祐、久後裕菜、財満信宏、森山達哉, 日本農芸化学会2017年度京都大会,   2017年03月18日
  • マウスを用いた卵白タンパク質の経皮感作モデル系の確立と高度化, 小川昂志, 高葢秋穂, 村上浩規, 岩本和子, 矢野えりか, 財満信宏, 森山達哉, 日本農芸化学会大会講演要旨集(Web),   2017年03月05日
  • キノコに含まれるシリンガ酸はエストロゲンレセプター非依存的に骨粗鬆症モデルマウスの骨密度減少を抑制する, 田中照佳, 川口信久, 財満信宏, 森山達哉, 福田泰久, 白坂憲章, 日本農芸化学会大会講演要旨集(Web),   2017年03月05日
  • 腹部大動脈瘤の進展・破裂に及ぼす高脂肪食の影響評価, 橋本佳祐, 久後裕菜, 財満信宏, 森山達哉, 日本農芸化学会大会講演要旨集(Web),   2017年03月05日
  • セサミン・セサモリンの脳内移行におよぼすウコン由来ターメロン高含有画分の影響, 松村 晋一, 吉岡 百合, 財満 信宏, 森本 正則, 村田 和也, 松田 秀秋, 日本薬学会年会要旨集,   2017年03月
  • アレルギー性鼻炎の免疫療法―最新知見―」に寄せる 汎アレルゲンであるチェリーのソーマチンライクプロテイン(Pru av2)の特性解析, 高葢秋穂, 村上浩規, 三口志穂, 矢野えりか, 岩本和子, 財満信宏, 森山達哉, 月刊アレルギーの臨床,   2017年02月20日
    概要:保存性が高く植物界に広く分布する汎アレルゲンは、全身症状や花粉症に関連したOASなどを引き起こす。汎アレルゲンの1つであるソーマチンライクプロテイン(PR-5)は、花粉にも含まれるが、OASを中心とした症状を惹起するのか、あるいは経腸的に吸収されて全身症状を惹起するのかいまだ十分に解明されていない。そこで、本研究ではサクランボやチェリーに含まれるソーマチンライクプロテイン(Pru av2)の特性を多様な観点から評価した。さらに、近年問題となっている新たな感作経路である経皮感作を起こしうるのかをマウスを用いて検討した。(著者抄録)
  • Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm, Hirona Kugo, Nobuhiro Zaima, Hiroki Tanaka, Youhei Mouri, Kenichi Yanagimoto, Kohsuke Hayamizu, Keisuke Hashimoto, Takeshi Sasaki, Masaki Sano, Tatsuro Yata, Tetsumei Urano, Mitsutoshi Setou, Naoki Unno, Tatsuya Moriyama, 第24回日本血管生物医学会学術集会,   2016年12月
  • 経口免疫寛容によって大豆タンパク質の経皮感作が抑制される, 村上浩規、高葢秋穂、小川昂志、矢野えりか、財満信宏、森山達哉,   2016年10月22日
  • 腹部大動脈瘤の進展・破裂に及ぼす高脂肪食の影響評価, 橋本佳祐、久後裕菜、財満信宏、森山達哉,   2016年10月22日
  • 腹部大動脈瘤の新規モデル動物の作出とその病理解析, 宮本智絵、橋本佳祐、久後裕菜、田中 宏樹、海野直樹、財満信宏、森山達哉,   2016年10月22日
  • 植物汎アレルゲンであるソーマチンライクプロテイン(PR-5)の特性解析, 高葢秋穂、三口志穂、小川昂志、村上浩規、矢野えりか、財満信宏、森山達哉, 日本栄養・食糧学会 第55回近畿支部大会,   2016年10月22日
  • 経皮感作される卵白タンパク質の解析, 小川昂志、高葢秋穂、村上浩規、矢野えりか、財満信宏、森山達哉 , 日本栄養・食糧学会 第55回近畿支部大会,   2016年10月22日
  • マウス眼球及び周囲組織におけるアントシアニン分子種の動態解析, 山田周平、荘厳哲哉、財満信宏、森山達哉, 日本栄養・食糧学会 第55回近畿支部大会,   2016年10月22日
  • The effect of high fat diet on development of abdominal aortic aneurysm, Keisuke Hashimoto, Hirona Kugo, Nobuhiro Zaima, Tatsuya Moriyama,   2016年10月19日
  • The effect of dietary food on the development of abdominal aortic aneurysm, Hirona Kugo, Nobuhiro Zaima, Youhei Mouri, Hiroki Tanaka, Kenichi Yanagimoto, Tetsumei Urano, Naoki Unno, Tatsuya Moriyama, The 1st Joint Meeting of ISFP and PA Workshop,   2016年10月19日
  • ウコン精油のβ-セクレターゼ阻害作用, 松村 晋一, 村田 和也, 吉岡 百合, 財満 信宏, 森本 正則, 岩城 正宏, 松田 秀秋, 日本生薬学会年会講演要旨集,   2016年08月
  • 食品による疾患の予防, 財満信宏, 近畿大学農学部 公開講座,   2016年05月21日, 招待有り
  • 腹部大動脈瘤発症に対する魚油の予防効果の検討, 久後裕菜、財満信宏、毛利陽平、田中宏樹、柳本賢一、浦野哲盟、海野直樹、森山達哉 , 第70回 日本栄養・食糧学会,   2016年05月15日
  • ヒト腹部大動脈瘤の病理解析と新規モデル動物の作出, 財満信宏、久後裕菜、田中宏樹、海野直樹、森山達哉, 第70回 日本栄養・食糧学会,   2016年05月15日
  • 低アレルゲン性新規食品素材としての大豆ホエイの特性解析, 植田啄也、和田宏章、矢野えりか、石黒貴寛、村澤久司、小川正、財満信宏、森山達哉 , 第70回 日本栄養・食糧学会,   2016年05月15日
  • 経皮感作し得る大豆タンパク質の検出及び同定, 村上浩規、小川祥平、矢野えりか、財満信宏、森山達哉, 第70回 日本栄養・食糧学会,   2016年05月15日
  • 虫害被害を受けた大豆(枝豆)のプロテオノーム・アレルゲノーム解析, 花房佳世、矢野えりか、末森祐輔、財満信宏、森山達哉, 第70回 日本栄養・食糧学会,   2016年05月15日
  • 低アレルゲン性新規食品素材としての大豆ホエイの特性解析, 植田 啄也, 和田 宏章, 矢野 えりか, 石黒 貴寛, 村澤 久司, 小川 正, 財満 信宏, 森山 達哉, 日本栄養・食糧学会大会講演要旨集,   2016年04月
  • 経皮感作し得る大豆タンパク質の検出及び同定, 村上 浩規, 小川 祥平, 矢野 えりか, 財満 信宏, 森山 達哉, 日本栄養・食糧学会大会講演要旨集,   2016年04月
  • 虫害被害を受けた大豆(枝豆)のプロテオーム・アレルゲノーム解析, 花房 佳世, 矢野 えりか, 末森 佑輔, 財満 信宏, 森山 達哉, 日本栄養・食糧学会大会講演要旨集,   2016年04月
  • お米の栄養はどこにある?目に見えない分子を可視化する技術ー質量分析イメージングの原理と実際-, 財満信宏, 機能性健康米協会シンポジウム,   2016年02月26日, 招待有り
  • 食品による疾患の予防, 財満信宏, 近畿大学農学部 公開講座,   2015年10月03日, 招待有り
  • アントシアニンの体内動態解析, 財満信宏、稲葉栞里、藤森隆道、荘厳哲哉、小林沙織、森山達哉, 第40回日本医用マススペクトル学会年会,   2015年09月18日
  • 経皮感作し得る大豆タンパク質の特性解析, 村上浩規、小川祥平、和田宏章、末森祐輔、矢野えりか、財満信宏、森山達哉, 日本食品科学工学会 第62回大会,   2015年08月29日
  • The preventive effect of fish oil on the development of abdominal aortic aneurysm, Hirona Kugo, Youhei Mouri, Ken-ichi Yanagimoto, Nobuhiro Zaima, Tatsuya Moriyama, 12th Asian Congress of Nutrition ,   2015年05月15日
  • Characteristic Distribution of Metabolites in Oryza sativa Rice, Nobuhiro Zaima, Yukihiro Yoshimura, Yukio Kawamura, and Tatsuya Moriyama, 106th AOCS Annual Meeting,   2015年05月03日
  • 新精米法による新たな機能性米の創出とその機能成分の可視化, 財満信宏、郡俊之、森山達哉、川西孝彦、寺下隆夫、白坂憲章, 日本農芸化学会2015年度大会,   2015年03月29日
  • イメージングマススペクトロメトリーの原理と分析事例, 財満信宏, 第45回中部化学関連学協会連合秋季大会,   2014年11月29日, 招待有り
  • 悪玉アディポサイトカインであるレジスチンの変動解析の再検討, 山本美里、田前 諒、山中大輔、矢野えりか、末森祐輔、鵜澤有希、財満信宏、森山達哉, 日本栄養・食糧学会 第53回近畿支部大会,   2014年10月
  • Nicotine投与がラット腹部大動脈血管壁に及ぼす影響の評価, 久後裕菜、財満信宏、森山達哉, 日本栄養・食糧学会 第53回近畿支部大会 ,   2014年10月
  • 農作物の収穫後処理による汎アレルゲンの変動解析, 三口志穂、矢野喜裕、矢野えりか、末森祐輔、鵜澤有希、財満信宏、小川 正、森山達哉, 日本栄養・食糧学会 第53回近畿支部大会,   2014年10月
  • イメージングマススペクトロメトリー(IMS)による卵黄脂質層構造の解析, 東埜恭明、財満信宏、森山達哉, 日本栄養・食糧学会 第53回近畿支部大会,   2014年10月
  • イメージングマススペクトロメトリーによる食品機能研究, 財満 信宏、森山達哉, 第59回食品新素材研究会,   2014年09月04日
  • 中性脂肪蓄積心筋血管症患者由来繊維芽細胞のTG代謝に及ぼすカルニチンの影響, 須見友子、鵜澤有希、平野賢一、財満信宏、森山達哉, 日本油化学会,   2014年09月
  • イメージング質量分析の食品科学への応用, 財満信宏, 第41回BMSコンファレンス,   2014年07月09日, 招待有り
  • イメージングマススペクトロメトリーによるスフィンゴ脂質の可視化, 財満信宏, 日本農芸化学会2014年度大会シンポジウム,   2014年03月, 招待有り
  • 腹部大動脈瘤発症に及ぼすn-3系高度不飽和脂肪酸の影響評価, 久後裕菜、毛利陽平、柳本賢一、韓力、財満信宏、森山達哉, 日本農芸化学会,   2014年03月
  • イメージングマススペクトロメトリーによる脂質の可視化, 財満信宏, 健康・長寿談話会,   2013年11月08日, 招待有り
  • イメージングマススペクトロメトリーによる機能性食品成分の可視化, 財満信宏, メタボロームシンポジウム,   2013年10月04日
  • 凍り豆腐の大豆クラス2アレルギーリスク低減化 の実証, 和田宏章、末森祐輔、矢野えりか、財満信宏、石黒貴寛、村澤久司、小川正、森山達哉、河村幸雄, 日本栄食・食糧学会 第52回近畿支部大会,   2013年10月
  • 豆味噌抽出物による破骨細胞分化抑制効果, 土岐倉千智、木村裕、田中照佳、鵜澤有希、財満信宏、森山達哉、河村幸雄, 日本栄食・食糧学会 第52回近畿支部大会,   2013年10月
  • 中性脂肪蓄積心筋血管症患者由来繊維芽細胞の脂質代謝に及ぼすカルニチンの影響, 須見友子、鵜澤有希、平野賢一、財満信宏、森山達哉、河村幸雄, 日本栄食・食糧学会 第52回近畿支部大会,   2013年10月
  • 大豆サポニンアグリコン(ソヤサポゲノール)の脂肪細胞分化抑制効果, 小林知世、境野眞善、山下貴稔、加茂修一、佐藤俊郎、財満信宏、森山達哉、河村幸雄, 日本栄食・食糧学会 第52回近畿支部大会,   2013年10月
  • イメージングマススペクトロメトリー-質量分析で脂質を見る-, 財満信宏, 日本油化学会,   2013年09月04日, 招待有り
  • 肥満・糖尿病モデルマウスに対する大豆サポニンの抗肥満効果, 小林知世、境野眞善、山下貴稔、加茂修一、佐藤俊郎、財満信宏、森山達哉、河村幸雄, 第60回日本食品科学工学会大会,   2013年08月30日
  • 新規大豆食品素材(大豆ペースト)の低アレルゲン性及び消化性の解析, 森山達哉、山田陽子、矢野えりか、崎川貴文、末森祐輔、財満信宏、片岡雅人、長瀬二郎、河村幸雄, 第60回日本食品科学工学会大会,   2013年08月30日
  • Ba-8 wx/ae二重変異体米の澱粉吸着型リゾホスファチヂルコリンの定量と胚乳部における局在解析(澱粉の構造・物性・利用,一般講演,日本応用糖質科学会平成25年度大会(第62回)), 庄野 陽祐, 小谷口 美也子, 藤原 美乃里, 中屋 慎, 財満 信宏, 北村 進一, 応用糖質科学 : 日本応用糖質科学会誌,   2013年08月20日
  • IMSによる組織内代謝変動の可視化-食品成分動態解析と病理解析への応用-, 財満信宏, 日本農芸化学会2013年度大会シンポジウム,   2013年03月27日, 招待有り
  • メタボローム可視化の生命科学への応用, 財満信宏, 生命医薬情報学連合大会,   2012年10月16日, 招待有り
  • ヒト腹部大動脈瘤の病理解析とモデル動物の新規作出, 毛利陽平、財満信宏、田中宏樹、森山達哉、河村幸雄、海野直樹、瀬藤光利, 日本食品科学工学会2012年度大会,   2012年08月
  • 脂肪細胞からのレジスチン分泌を抑制する食品成分の探索, 若木泰子、吉村征浩、鵜澤有希、財満信宏、森山達哉、河村幸雄, 第29回日本微量栄養素学会学術集会,   2012年06月
  • クロマグロ皮たんぱく質経口摂取によるマウスの肝機能保護作用, 髙橋賢次、田中照佳、岩本直樹、阿川泰夫、澤田好史、吉村征浩、財満信宏、森山達哉、河村幸雄, 日本農芸化学大会,   2012年03月
  • ザクロ抽出物およびエラグ酸によるタンパク質分解亢進を介したレジスチン分泌抑制効果, 若木泰子、吉村征浩、鵜澤有希、財満信宏、森山達哉、河村幸雄, 日本農芸化学会大会,   2012年03月
  • 新規の腹部大動脈瘤モデルラットの作出と病理解析, 毛利陽平、財満信宏、田中宏樹、森山達哉、河村幸雄、海野直樹、瀬藤光利, 日本農芸化学会2012年度大会,   2012年03月
  • 経口摂取プエラリンのマウス骨吸収抑制作用と大腿骨への移行, 田中照佳、吉村征浩、道原成和、山本彩実、財満信宏、森山達哉、河村幸雄, 日本農芸化学大会,   2012年03月
  • IMSによるプエラリンの体内動態解析, 山本彩実、財満信宏、吉村征浩、田中照佳、森山達哉、河村幸雄, 日本農芸化学大会,   2012年03月
  • IMS解析が明らかにするアントシアニンの分子種による組織内局在の違い, 吉村征浩、財満信宏、森山達哉、河村幸雄, 日本農芸化学大会,   2012年03月
  • イメージングマススペクトロメトリーによる農作物の機能性食品成分の局在解析, 財満信宏、吉村征浩、森山達哉、河村幸雄, 日本農芸化学大会,   2012年03月
  • SF-051-2 一次性下肢静脈瘤の弁不全機序に関与する脂質分子の同定(血管-2,サージカルフォーラム,第110回日本外科学会定期学術集会), 田中 宏樹, 海野 直樹, 山本 尚人, 相良 大輔, 鈴木 実, 西山 元啓, 眞野 勇記, 佐野 真規, 財満 信宏, 佐々木 健, 瀬藤 光利, 日本外科学会雑誌,   2010年03月05日
  • OP-241 腎癌の脂質プロファイリング(腎腫瘍/基礎2,一般演題口演,第98回日本泌尿器科学会総会), 高山 達也, 財満 信宏, 宮崎 美紀, 高岡 直央, 京野 陽子, 伊藤 寿樹, 杉山 貴之, 永田 仁夫, 古瀬 洋, 栗田 豊, 麦谷 荘一, 瀬藤 光利, 大園 誠一郎, 日本泌尿器科学会雑誌,   2010年

特許

  • 大動脈瘤の予防または治療剤および容器詰飲食品, 財満 信宏, 毛利 陽平, 柳本 賢一, 韓 力, JP2014068333, WO2015-005393
  • 糖尿病性心血管合併症の予防・治療剤, 平野 賢一, 安井 洋子, 池田 善彦, 瀬藤 光利, 財満 信宏, JP2012071594, WO2013-031729
  • 糖尿病性心血管合併症の予防・治療剤, 平野 賢一, 安井 洋子, 池田 善彦, 瀬藤 光利, 財満 信宏, 特願2013-531304, 特許第5810166号
  • 大動脈瘤モデル動物, 海野 直樹, 田中 宏樹, 眞野 勇記, 佐野 真規, 財満 信宏, 瀬藤 光利, 佐々木 健, 特願2011-105426, 特開2012-235715
  • PPARγ阻害剤を用いる動脈瘤の予防および治療, 瀬藤 光利, 財満 信宏, 海野 直樹, 田中 宏樹, JP2010004570, WO2011-007565

競争的資金

  • 文部省科学研究費 (基盤研究B), 腹部大動脈瘤の進展・破裂予防法の確立, 財満信宏
  • 文部科学省科研費 (若手研究A), 質量顕微鏡を用いた病態検査法の開発, 財満信宏