 | 伊藤 仁久 （イトウ キミヒサ）
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葉や花などの未利用農産資源から生活習慣病予防に有用な化粧品・サプリメントの素材探索研究に従事。これまで、マンゴー葉に老化予防、ウンシュウミカン葉に肥満予防、摘果柑橘果実にメラニン産生抑制、抗アレルギー効果等を見出している。報道関連出演・掲載一覧
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研究キーワード
- 生活習慣病 終末糖化産物 (AGEs) メラニン リパーゼ阻害
現在の研究分野（キーワード）
- 葉や花などの未利用農産資源から生活習慣病予防に有用な化粧品・サプリメントの素材探索研究に従事。これまで、マンゴー葉に老化予防、ウンシュウミカン葉に肥満予防、摘果柑橘果実にメラニン産生抑制、抗アレルギー効果等を見出している。
研究分野
所属学協会
研究活動情報
論文
- 精油30種の終末糖化産物（AGEs）産生抑制およびチロシナーゼ阻害効果伊藤仁久; 大西雄己; 三澤紅; 藤阪芽以; 友廣教道; 松川哲也; 遠藤雄一; 梶山慎一郎; 重岡成アロマテラピー学雑誌 24 2 14 - 20 2023年05月 [査読有り]
- Kosuke Shimizu; Tetsuya Matsukawa; Risa Kanematsu; Kimihisa Itoh; Shinya Kanzaki; Shigeru Shigeoka; Shin'ichiro KajiyamaBioscience, Biotechnology, and Biochemistry 85 8 1789 - 1797 2021年07月 [査読有り]
ABSTRACT Headspace solid-phase microextraction combined with gas chromatography/mass spectrometry is one of the strongest tools for comprehensive analysis of volatile compounds and has been used to analyze aromatic components of mango and investigate its varietal characteristics. In this study, profiling of aroma compounds in 17 mango cultivars, grown in the same green house to exclude the effect of environmental factors, was conducted and the patterns were subjected to principal component analysis (PCA) to identify the relationship between the aroma components and cultivars. Fifty-nine different volatile constituents were detected from the blends of these 17 mango cultivars. The cultivars were divided into 4 clusters using PCA based on the volatile components determined in the study. Aiko was found to mainly contain δ-3-carene and showed a composition more similar to its pollen parent, Irwin, than to its seed parent, Chiin Hwang No. 1. - Kimihisa Itoh; Tetsuya Matsukawa; Takahiro Deguchi; Momona Yamagami; Norimichi Tomohiro; Kazuya Murata; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru ShigeokaJournal of Plant Studies 10 2 1 - 1 2021年04月 [査読有り]
During the course of Citrus unshiu fruit cultivation, large amounts of plant material including pruned leaves, thinned-out flowers and unripe fruit are obtained; these materials are generally considered unusable and discarded as plant wastes. We have previously attempted to establish an effective use of such plant wastes as beneficial natural resources and found that a methanolic extract of pruned Citrus leaves (CUL-ext) exhibited inhibitory activity against porcine pancreatic lipase. In this study, we sought to identify further the effective uses of C. unshiu plant wastes by determining the lipase inhibitory activity of methanolic extracts of thinning out flowers (CUF-ext) and unripe fruit (CUUF-ext). We accordingly found that the inhibitory activity of CUF-ext was superior to that of CUUF-ext and comparable to that previously observed using CUL-ext. Fractionation of CUF-ext and CUUF-ext, followed by chromatographic analyses, revealed that the pancreatic lipase inhibitory activities of these extracts could be attributed, at least in part, to the flavonoids hesperidin, nobiletin, narirutin and rutin. On the basis of present findings, we propose that, in addition to pruned Citrus leaves, the thinned-out flowers and unripe fruit of C. unshiu are natural resources which are suitable for preparing constituents with lipase inhibitory activity. - Kimihisa Itoh; Tetsuya Matsukawa; Takahiro Deguchi; Momona Yamagami; Norimichi Tomohiro; Kazuya Murata; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru ShigeokaJournal of Plant Studies 10 2 1 - 7 2021年04月 [査読有り]
This study examines the effect of quantity sold (sales volume) on profitability of market participating smallholder farmers in northern Ghana. Market participation has been shown to be important for increasing incomes and improving production efficiency for farm households but still remains low in SSA. While agribusiness and development experts generally advocate for more intensive market participation, it is not clear if selling more results in more profits for smallholder farmers in remote markets that are prone to exorbitant transaction costs. The data used in this study is from the APS survey conducted in 2013 and 2014 in Northern Ghana which had a sample size of 527. The study is based on the theory of profit maximization, in which separability is inferred from observed market participation. OLS regression is used for empirical estimation after rejecting the hypothesis of endogeneity in the model. Mean gross margin/ kg across four groups of farmers ranked by quantity sold is also statistically examined. The results confirm the existence of economies of scale and also show that different crops have different effects on profitability. The results also show that although unambiguously positive, the relationship between quantity sold and profitability may not be linear. - Kimihisa Itoh; Tetsuya Matsukawa; Mamoru Okamoto; Kanasa Minami; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru ShigeokaJournal of Plant Studies 9 2 39 - 45 2020年06月 [査読有り]
In this study, we aimed to identify the utility of pruned mango (Mangifera indica ‘Irwin’) leaves as a resource for ingredients with antioxidant activity. Firstly, we examined the antioxidant activity of extracts obtained from the pericarps, flesh, flowers, barks, seeds, young dark reddish brown leaves (YDL-ext), young yellow leaves (YYL-ext), and pruned old dark green leaves (OML-ext) obtained from ‘Irwin’ mango. Among them, methanolic extract of flower and OML-ext showed the most potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity. The flesh extract showed weak DPPH radical scavenging activity, but did not show SOD-like activity. Secondly, we investigated the relationship between the maturation of leaves and their antioxidant activity by considering the contents of their two active polyphenolic components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1) and mangiferin (2), in addition to chlorophyll (3) and anthocyanins represented by cyanidin-3-O-glucoside (4). The DPPH radical scavenging activity of YDL-ext, YYL-ext and OML-ext were mainly attributable to 1, 2 and 3, whereas their SOD-like activity was partly attributable to 2. The DPPH radical scavenging and SOD-like activities of YDL-ext and YYL-ext were attributable to 1 and 2. These activities were also due to anthocyanins whose content is highest in YDL-ext. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for preparing cosmetics and/or supplemental ingredients with health-enhancing properties, antioxidant activity and inhibitory effect on AGEs formation and pancreatic lipase. - 志水恒介; 巽賢太郎; 宇都宮直樹; 神崎真哉; 伊藤仁久; 重岡成園芸学研究 20 1 87 - 94 一般社団法人 園芸学会 2020年06月 [査読有り]
マンゴー ‘愛紅’ は ‘アーウィン’ と比べて隔年結果性が強く，大果性の晩生品種である．本研究では，カンキツの栽培に適用されている隔年交互結実栽培法をマンゴー ‘愛紅’ に応用し，その有効性を検討した．交互結実処理には主枝別交互結実区と樹別交互結実区の2処理区を設け，慣行法の対照区と比較した．花穂発生率に対照区と処理区間で有意な差はみられなかったが，ON-branchおよびON-treeの花穂発生率は安定して高く，ほぼすべての枝から花穂が発生した．両交互結実処理区の収量は対照区と有意差がなかったが，平均果実重は対照区より軽くなり，小玉果の割合が高くなった．作業効率に関し，両交互結実処理区ではせん定時間が短縮され省力化が可能であることが示唆された．一方，MiFT遺伝子の発現量は ‘愛紅’ の隔年結果性と関連があることが示され，開花数の予測に利用可能であることが示唆された．マンゴー ‘愛紅’ において，隔年交互結実栽培は慣行法と同程度の果実品質と収量を維持しながら，生産量の変動制御と省力化を可能にする有効な方法であると考えられた． - Kimihisa Itoh; Tetsuya Matsukawa; Kanasa Minami; Mamoru Okamoto; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru ShigeokaJournal of Plant Studies 9 2 33 - 38 2020年05月 [査読有り]
As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation. - Kimihisa Itoh; Tetsuya Matsukawa; Kazuya Murata; Ryota Nishitani; Momona Yamagami; Norimichi Tomohiro; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Shigeru Shigeoka; Yuichi Endo; Hideaki MatsudaNatural Product Communications 14 9 1934578X1987343 - 1934578X1987343 2019年09月 [査読有り]
In the cultivation of Citrus fruit, pruning in late winter after harvest is important to obtain excellent flower buds in the next spring and a rich harvest of superior fruit in the following winter; a large number of pruned leaves are usually discarded. The purpose of this study was to determine the utility value of pruned leaves of Citrus unshiu. A methanol extract of pruned C. unshiu leaves showed a porcine pancreatic lipase inhibitory activity. Fractionation of C. unshiu leaf extract (CUL-ext) followed by bibliographic and chromatographic analyses revealed that a part of the pancreatic lipase inhibitory activity of CUL-ext was attributable to nobiletin (1), rutin (2), and hesperidin (3). Thus, pruned C. unshiu leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity. - Kimihisa Itoh; Kazuya Murata; Nao Sakaguchi; Kohei Akai; Tomoka Yamaji; Kohsuke Shimizu; Kaoru Isaki; Tetsuya Matsukawa; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki MatsudaJournal of plant studies 6 2 102 - 107 2017年08月 [査読有り]
The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation. - Inhibitory activity of Citrus madurensis ripe fruits extract on antigen-induced degranulation in RBL-2H3 cellsKimihisa Itoh; Kazuya Murata; Megumi Futamura-Masuda; Takahiro Deguchi; Yuko Ono; Marin Eshita; Masahiko Fumuro; Morio Iijima; Hideaki MatsudaJournal of plant studies 6 1 23 - 30 2017年04月 [査読有り]
- A pancreatic lipase inhibitory activity by Mango (Mangifera indica) leaf methanolic extractKimihisa Itoh; Kazuya Murata; Yuta Nakagaki; Ayaka Shimizu; Yusuke Takata; Kohsuke Shimizu; Tetsuya; Matsukawa; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki MatsudaJournal of plant studies 5 2 72 - 78 2016年07月 [査読有り]
- Combined effect of lysed Enterococcus faecalis FK-23 and fruits of Citrus unshiu on contact dermatitis in miceNishikawa K; Shimada T; Hayashi A; Okamori M; Fukuda K; Goshima A; Itoh K; Murata K; Matsuda HPharmacometrics 90 5-6 95 - 99 2016年 [査読有り]
- Kazuya Murata; Keisuke Takahashi; Haruka Nakamura; Kimihisa Itoh; Hideaki MatsudaNATURAL PRODUCT COMMUNICATIONS 9 2 185 - 188 2014年02月 [査読有り]
In our research program for discovering novel skin-whitening materials, screening of extracts from flowers of some Prunus species was performed using an anti-tyrosinase assay. Among the tested plants, the flowers of P. persica showed the most potent inhibitory activity. In addition, P. persica also showed suppression of melanogenesis in B16 rat melanoma cells. The active principles of tyrosinase inhibition and suppression of melanogenesis were revealed to be an afzelin (3-O-alpha-L-rhamnosylkaempferol) and a flavanone, naringenin. The mechanism of the anti-melanogenesis effect of these two compounds was disclosed, for the first time, as the suppression of the expression of tyrosinase protein, which was controlled by the inhibition of phosphorylation of p38 MAPK. These findings show that these compounds could be candidates for the novel molecular target for a skin-whitening agent. - Megumi Masuda; Kimihisa Itoh; Kazuya Murata; Shunsuke Naruto; Akemi Uwaya; Fumiyuki Isami; Hideaki MatsudaBIOLOGICAL & PHARMACEUTICAL BULLETIN 35 1 78 - 83 2012年01月 [査読有り]
The objective of this study was to examine the effects of Morinda citrifolia (noni) extract and its constituents on a-melanocyte stimulating hormone (alpha-MSH)-stimulated melanogenesis in cultured murine B16 melanoma cells (B16 cells). A 50% ethanolic extract of noni seeds (MCS-ext) showed significant inhibition of melanogenesis with no effect on cell proliferation. MCS-ext was more active than noni leaf and fruit flesh extracts. Activity guided fractionation of MCS-ext led to the isolation of two lignans, 3,3'-bisdemethylpinoresinol (1) and americanin A (2), as active constituents. To elucidate the mechanism of melanogenesis inhibition by the lignans, alpha-MSH-stimulated B16 cells were treated with 1 (5 mu m) and 2 (200 mu m). Time-dependent increases of intracellular melanin content and tyrosinase activity, during 24 to 72h, were inhibited significantly by treatment with the lignans. The activity of 1 was greater than that of 2. Western blot analysis suggested that the lignans inhibited melanogenesis by down regulation of the levels of phosphorylation of p38 mitogen-activated protein kinase, resulting in suppression of tyrosinase expression. - Kikuyo Nakao; Kazuya Murata; Takahiro Deguchi; Kimihisa Itoh; Takanori Fujita; Masayuki Higashino; Yuri Yoshioka; Shin-ichi Matsumura; Rika Tanaka; Tetsuro Shinada; Yasufumi Ohfune; Hideaki MatsudaBIOLOGICAL & PHARMACEUTICAL BULLETIN 34 7 1143 - 1146 2011年07月 [査読有り]
Kaempferia parviflora (KP), a Zingiberaceae plant, is used as a folk medicine in Thailand for the treatment of various symptoms, including general pains, colic gastrointestinal disorders, and male impotence. In this study, the inhibitory activities of KP against xanthine oxidase (XOD) were investigated. The extract of KP rhizomes showed more potent inhibitory activity (38% at 500 mu g/ml) than those of the other Zingiberaceae plants tested. Ten methoxyflavones were isolated from the KP extract as the major chemical components and their chemical structures were elucidated by X-ray crystallography. The structurally confirmed methoxyflavones were subjected to the XOD inhibitory test. Among them, 3,5,7,4',5'-pentamethoxyflavone and 3',4',5,7-tetramethoxyflavone showed inhibitory activities (IC50 of 0.9 and >4 mM, respectively) and their modes of inhibition are clarified as competitive/non-competitive mixed type. To the best of our knowledge, this is the first report to present the inhibitory activities of KP, 3,5,7,4',5'-pentamethoxyflavone and 3',4',5,7-tetramethoxyflavone against XOD. - Megumi Masuda; Kazuya Murata; Kimihisa Itoh; Shunsuke Naruto; Akemi Uwaya; Fumiyuki Isami; Hideaki MatsudaJournal of Traditional Medicines 28 2 47 - 54 2011年 [査読有り]
Functional foods derived from noni (Morinda citrifolia) have been marketed to help prevent lifestyle-related diseases. Many lifestyle-related diseases, such as hypertension and hyperlipemia, are associated with poor blood fluidity. The objective of this study was to examine the effect of 50% ethanolic extracts from noni, and their constituents, on blood fluidity, in a lipopolysaccharide (LPS)-induced disseminated intravascular coagulation (DIC) rat model with a micro channel array flow analyzer (MC-FAN). Oral administrations of the noni leaf extract (MCL-ext) and the noni seed extract (MCS-ext) to DIC rats resulted in a reduction of the passage time of whole blood, as compared to the vehicle control. The MCL-ext and its constituents, namely ursolic acid (1), rutin (2), and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (3) inhibited collagen-induced platelet aggregation. MCL-ext, as well as MCS-ext and its constituents, namely 1, americanin A (4), and 3, 3′-bisdemethylpinoresinol (5), inhibited polybrene-induced erythrocyte aggregation. Inhibition of erythrocyte aggregation by MCL-ext and MCS-ext was mainly attributable to 1. The euglobulin lysis time (ELT) assay indicated that oral administration of MCL-ext or MCS-ext induces fibrinolysis activity in rats. This is the first report of the effects of noni extracts on blood fluidity. MCL-ext exhibited more in vitro anti-platelet activity, and improvement of blood fluidity and fibrinolysis activity in vivo than MCS-ext. © 2011, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved. - Kikuyo Nakao; Kazuya Murata; Kimihisa Itoh; Yuko Hanamoto; Megumi Masuda; Kenzo Moriyama; Takahiro Shintani; Hideaki MatsudaJournal of Traditional Medicines 28 1 10 - 15 2011年 [査読有り]
In our search for novel materials possessing anti-hyperuricemic effects, we examined immature Citrus unshiu fruit. Extracts of immature C. unshiu fruit showed moderate anti-hyperuricemic effects in a hyperuricemic model the extract reduced increased serum urate level by 36% at 500 mg/kg, p.o. The extract did not show significant xanthine oxidase inhibitory activity, but the metabolite of the main flavanone glycoside in the fruit, hesperetin, showed potent anti-hyperuricemic effects hesperetin reduced increased serum urate level by 44% at 200 mg/kg, p.o. Furthermore, hesperetin showed dose-dependent xanthine oxidase inhibitory activity of 52% at 200 μM. Thus, the anti-hyperuricemic effects of immature C. unshiu may be attributed to hesperetin, a metabolite of hesperidin via xanthine oxidase inhibition. © 2011, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved. - Kimihisa Itoh; Megumi Masuda; Shunsuke Naruto; Kazuya Murata; Hideaki MatsudaBIOLOGICAL & PHARMACEUTICAL BULLETIN 33 4 659 - 664 2010年04月 [査読有り]
The enhancement of blood fluidity may lead to improvements in skin problems resulting from unsmooth circulation or blood stagnation. Since a 50% ethanolic extract (CH-ext) obtained from unripe Citrus hassaku fruits may be a useful ingredient in skin-whitening cosmetics, the present study was designed to examine the effect of CH-ext on blood fluidity. CH-ext concentration-dependently inhibited in vitro collagen-induced rabbit platelet aggregation and in vitro polybrene-induced rat erythrocyte aggregation. The CH-ext showed in vitro fibrinolysis activity in fibrin plate assay. Activity-guided fractionation of the CH-ext using antiplatelet activity, inhibitory activity of erythrocyte aggregation, and fibrinolysis activity revealed that these activities of CH-ext were attributable to naringenin-7-glycoside (prunin). Successive oral administration of CH-ext to rats inhibited the lipopolysaccharide (LPS)-induced decrease of blood platelets and fibrinogen, and LPS-induced increase of fibrin degradation products (FDP) in LPS-induced disseminated intravascular coagulation (DIC) model rats. Effects of CH-ext on blood fluidity were analyzed by a micro channel array flow analyzer (MC-FAN). Preventive oral administration of CH-ext to rats showed dose-dependent reduction of the passage time of whole blood flow of the DIC model rats in comparison with that of the vehicle control rats. These results imply that CH-ext may have effects which improve effects on blood fluidity. - Kimihisa Itoh; Megumi Masuda; Shunsuke Naruto; Kazuya Murata; Hideaki MatsudaJOURNAL OF NATURAL MEDICINES 63 4 443 - 450 2009年10月 [査読有り]
Oral administration of a 50% ethanolic extract (CH-ext) obtained from unripe Citrus hassaku fruits collected in July exhibited a potent dose-dependent inhibition of IgE (immunoglobulin E)-mediated triphasic cutaneous reaction at 1 h [immediate phase response (IPR)], 24 h [late phase response (LPR)] and 8 days [very late phase response (vLPR)] after dinitrofluorobenzene challenge in mice. Naringin, a major flavanone glycoside component of CH-ext, showed a potent dose-dependent inhibition against IPR, LPR and vLPR. Neohesperidin, another major glycoside component of CH-ext, showed an inhibition against vLPR. The effect of CH-ext on type IV allergic reaction was examined by determining inhibitory activity against ear swelling in mice by using the picryl chloride-induced contact dermatitis (PC-CD) model. Oral administration (p.o.) of CH-ext and subcutaneous administration (s.c.) of prednisolone inhibited ear swelling during the induction phase of PC-CD. The inhibitory activities of combinations of CH-ext (p.o.) and prednisolone (s.c.) against PC-CD in mice were more potent than those of CH-ext alone and prednisolone alone, without enhancing the adverse effects. Other combinations of prednisolone (s.c.) and flavanone glycoside (p.o.) components of CH-ext, i.e. naringin and neohesperidin, exerted similar synergistic effects. - Kimihisa Itoh; Noriko Hirata; Megumi Masuda; Shunsuke Naruto; Kazuya Murata; Keitaro Wakabayashi; Hideaki MatsudaBIOLOGICAL & PHARMACEUTICAL BULLETIN 32 3 410 - 415 2009年03月 [査読有り]
The 50% ethanolic extract (CH-ext) obtained from the unripe fruit of Citrus hassaku exhibited significant tyrosinase inhibitory activity. The CH-ext showed antioxidant activity, such as superoxide dismutase (SOD)-like activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Activity-guided fractionation of the CH-ext indicated that flavanone glycoside-rich fractions showed potent tyrosinase inhibitory activity. Further examination revealed that the tyrosinase inhibitory activity and antioxidant activity of the CH-ext were attributable to naringin and neohesperidin, respectively. The CH-ext showed inhibition of melanogenesis without any effects on cell proliferation in cultured murine B16 melanoma cells after glucosamine exposure. The topical application of the CH-ext to the dorsal skin of brownish guinea pigs showed in vivo preventive effects against UVB-induced pigmentation. - Kozo Fukuda; Kazuya Murata; Kimihisa Itoh; Hideaki Matsuda; Masahiko Taniguchi; Makio Shibano; Kimiye Baba; Makoto Shiratori; Tadato TaniJournal of Traditional Medicines 26 42496 210 - 218 2009年The dried roots of Angelica acutiloba (Yamato-toki in Japanese) are an important crude drug component of traditional Chinese prescriptions used to improve menopausal symptoms. The improvement in blood circulation by Yamato-toki was revealed by the oral administration of (Z)-ligustilide, a major ingredient of Yamato-toki, which showed significant shortening of euglobulin lysis time. This is the first report to reveal fibrinolytic activity of (Z)-ligustilide. Furthermore, ferulic acid and adenosine were two active ingredients with antiplatelet, antierythrocyte aggregation, and fibrinolytic activities. A. acutiloba roots are processed into Yamato-toki by Yumomi treatment, which is a traditional processing procedure involving blanching in hot water. Methanol extracts of A. acutiloba roots with or without Yumomi treatment showed similar dose-dependent and significant antierythrocyte aggregation activities. There was no discernable difference in the (Z)-ligustilide, ferulic acid, and adenosine contents between A. acutiloba roots with or without Yumomi treatment. Sugar analysis results indicated that Yumomi processing increases the monosaccharide content, which enhances sweetness. The results showed that (Z)-ligustilide was a fibrinolytic activator. However, there was no enhancement of biological activity following Yumomi treatment. Thus, Yumomi is utilized mainly to improve appearance and quality, as evaluated by sensory tests, and to enhance sweetness and brownish color rather than raising the contents of ingredients. © 2009, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved.
- Noriko Hirata; Shunsuke Naruto; Kazunori Inaba; Kimihisa Itoh; Masashi Tokunaga; Munekazu Iinuma; Hideaki MatsudaBIOLOGICAL & PHARMACEUTICAL BULLETIN 31 10 1973 - 1976 2008年10月 [査読有り]
Oral administration of a methanolic extract of Piper nigrum leaf (PN-ext, 50, 200 and 500 mg/kg) showed a potent dose-dependent inhibition of dinitrofluorobenzene (DNFB)-induced cutaneous reaction at I h [immediate phase response (IPR)] after and 24 h [late phase response (LPR)] after DNFB challenge in mice which were passively sensitized with anti-dinitrophenyl (DNP) IgE antibody. Ear swelling inhibitory effect ot PN-ext (50, 200 and 500 mg/kg, per os (p.o.)) on very late phase response (vLPR) in the model mice was significant but weaker than that on IPR. Oral administration of PN-ext (50, 200 and 501) mg/kg for 7 d) inhibited picryl chloride (PC)-induced ear swelling in PC sensitized mice. PN-ext exhibited in vitro inhibitory effect on compound 48/80-induced histamine release from rat peritoneal mast cells. Two lignans of PN-ext, (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2), were identified as major active principles having histamine release inhibitory activity. - 平田 規子; 岡本 美保; 伊藤 仁久; 稲葉 一訓; 松田 秀秋; 得永 雅士生薬学雑誌 62 2 66 - 71 日本生薬学会 2008年On the basis of a literature search on classical Chinese herbal and medicinal books and recent Chinese medical and pharmaceutical journals, I selected several crude drugs which have been used for the treatment of alopecia, thermal burn, benign prostatic hyperplasia, tonics, and dandruff prevention. Testosterone 5α-reductase inhibitory activity of the selected crude drugs and other randomly selected crude drugs was assayed to find novel agents for treatment of androgenic alopecia. The ethanolic solutions of Schisandra chinensis BAlLLON (五味子), Spatholobus suberectus DUNN (鶏血藤), Polygonum bistorta L. (拳参), Lygodium japonicum (THUBN.) Sw. (海金砂), Psoralea corylifolia L. (補骨脂), Myrica rubra SIEB. et ZUCC. (揚梅皮), Cornus officinalis SIEB. et ZUCC. (山茱萸), and Paeonia suffruticosa ANDR. (牡丹皮) showed strong inhibition of the enzyme activity.
- Yasuyuki Tsukioka; Kikuyo Nakao; Kenzo Moriyama; Takahiro Shintani; Keiichi Samukawa; Kimihisa Itoh; Fumiaki Takeshita; Hideaki MatsudaJournal of Traditional Medicines 25 2 43 - 51 2008年The improving effects of 70% methanol extract (RG-ext) and its potent components obtained from Red Ginseng on the peripheral circulation disorder were investigated using various experimental models in rats. RG-ext promoted the dorsal skin blood flow of intact rat and improved the lowering of the dorsal skin blood flow induced by norepinephrine (NE), and also caused by water immersion, but was ineffective on serotonin-treated rats. RG-ext restored the increase of the blood flow induced by doxazosin (DO). Ginsenoside-Rb1 (Rb1) improved the lowering of the dorsal skin blood flow by NE and ginsenoside-Rg1 (Rg1) restored the increase of blood flow by DO. When Rb1 was administered orally to rats in combination with non-saponin fraction (RW-fr) obtained from Red Ginseng, the improving effects were more enhanced than that of Rb1 alone, but it was ineffective in RW-fr itself. Non-saponin fraction (WW-fr) from White Ginseng did not show the increasing effect such a RW-fr. Rb1 produced 20(S)- and 20(R)-ginsenoside-Rg3 [20(S)- and 20(R)-Rg3] in gastric juice, and in artificial gastric juice. Ginsenoside-Rb2, -Rc and -Rd also produced 20(S)- and 20(R)-Rg3 as well as Rb1. When 20(S)-Rg3 was administered intravenously to rats, it improved the lowering of the dorsal skin blood flow induced by NE, but only showed a tendency to improve the blood flow in case of oral administration. 20(S)-Rg3 showed relatively strong improving effect in combination with RW-fr. Consequently, it has become apparent that the improving effect of RG-ext on the peripheral circulation disorder are attributable to action of 20(S)-Rg3 which was formed from protopanaxadiol saponins of RG-ext in stomach and then absorbed into blood flow through intestinal membrane with some support of RW-fr. © 2008, Medical and Pharmaceutical Society for WAKAN-YAKU. All rights reserved.
講演・口頭発表等
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- ショウガ科植物Kaempferia parviflora 根茎のキサンチンオキシダーゼ阻害作用 [通常講演]村田和也; 出口貴浩; 伊藤仁久; 松田秀秋; 中尾紀久世; 新谷卓弘; 藤田貴則; 東野正行; 吉岡百合; 松村晋一; 田中里佳; 品田哲郎; 大船泰史日本薬学会第131年会 2011年03月
- Anti-allergy effect of extract from fruits [通常講演]Kazuya Murata; Megumi Masuda; Kimihisa Itoh; Hideaki Matsuda2010 International Chemical Congress of Pacific Basin Societies 2010年12月
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MISC
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- 松田 秀秋; 伊藤 仁久; 増田 めぐみ 香料 (247) 63 -71 2010年
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