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KOBAYASHI Toru
Department of Fisheries | Professor/Manager |
Last Updated :2024/08/31
■Researcher comments
List of press-related appearances
1
■Researcher basic information
Degree
J-Global ID
Research Keyword
- Amago salmon Rainbow trout Gynogenetic Breeding Genome Editing Morphogenesis Early development chimera Primordial germ cell Cell transplanting Willow minnow Clone Sex differentiation Reproduction Breeding Polyploidy 染色体操作 Chromosome manipulation
Research Field
■Career
Career
- 2012/04 - Today Lab. for Aquatic Biology, Faculty of Agriculture, Kinki UniversityProfessor
- 2007/04 - 2012/03 Kindai UniversityFaculty of Agriculture
- 2004/04 - 2007/03 Lab. for Aqauctic Biology, Faculty of Agriculture, Kinki UniversityAsistant professor
- 2000/04 - 2004/03 Lab. for Aqauctic Biology, Faculty of Agriculture, Kinki UniversityLecturer
- 1997/04 - 2000/03 Lab. for Aqauctic Biology, Faculty of Agriculture, Kinki UniversityReearch asociate
- 1993/04 - 1997/03 Shiga prefecture fisheries experimental station増殖係senior technical staff
- 1990/04 - 1993/03 Shiga prefecture Samegai Trout Farmsenior technical staff
- 1985/04 - 1990/03 Shiga prefecture, Samegai Trout FarmTechnical staff
■Research activity information
Award
Paper
- Toru Kobayashi; Akito Takeda; Kanta HaraAquaculture Research Hindawi Limited 2023 1 - 17 1355-557X 2023/02 [Refereed]
Doubled haploids (DHs) are essential founders for breeding via isogenic line production. We determined the optimal timing of the first and second (HST1 and HST2) double heat shock treatment for DH production in the willow gudgeon (Gnathopogon caerulescens). Gynogenesis was induced by fertilisation with UV-irradiated sperm (20 mJ/cm2). Each HST was performed at 40.5°C for 1 min. In experiment I, HST1 was applied 23–31 min after the induction of gynogenetic development (incubated at 20°C). HST2 was performed 15 min after HST1 treatment. When HST1 was started 25–26 min after activation, normally hatched larvae appeared relatively frequently (35.6–100%), and most were DHs. In experiment II, HST1 was started 26 min after activation, and HST2 was started 10–25 min after HST1. Starting HST2 10 min after HST1 resulted in 33% more normally hatched larvae than starting 22.5–25 min after. DHs were prevalent among hatched larvae (45.1% and 20.8%, respectively) when performing HST2 10–12.5 min after HST1. Under appropriate HST1 and HST2 timing, gynogens were at the zygote stage, early prophase, and prophase or early prometaphase of the first cell cycle. These results establish the appropriate timing of HST1 and HST2 for the induction of willow gudgeon DHs. - Yu Murakami; Masashi Ando; Ryota Futamata; Tomohisa Horibe; Kazumitsu Ueda; Masato Kinoshita; Toru KobayashiScientific Reports Springer Science and Business Media LLC 12 (1) 18588 2022/11 [Refereed]
Abstract Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to its umami taste. An attractive strategy for enhancing umami is to suppress the IMP-degrading activity and increase the IMP content in the skeletal muscle through genome editing technology using the CRISPR-Cas9 system. However, the molecular mechanisms underlying IMP degradation remain unclear. We cloned two ecto-5′-nucleotidase genes, designated as ecto-5′-nucleotidase-a (nt5ea) and ecto-5′-nucleotidase-b (nt5eb), from medaka (Oryzias latipes), a vertebrate model organism. Expression analysis using embryos showed that nt5ea or nt5eb overexpression remarkably upregulated IMP degradation, and that the IMP-degrading activity was higher in Nt5ea than in Nt5eb. Furthermore, we established frame-shifted or large deletion (lacking nt5ea or nt5eb locus) mutant strains and assayed the effects of gene disruptions on the amount of IMP in skeletal muscle. The nt5ea-deficient medaka showed considerable higher levels of IMP at 48 h postmortem than did the wild-type fish. The nt5eb mutants also exhibited higher IMP contents than that in the wild types, but the increase was less than that in the nt5ea mutants. Our results demonstrated that nt5e is an important regulator of IMP levels in skeletal muscle and that its loss of function was effective in maintaining IMP content. - Yu Murakami; Toru Kobayashigenesis Wiley 1526-954X 2022/01 [Refereed]
- KOBAYASHI Toru; ASHIDA HiroshiAquaculture Science 67 (1) 9 - 17 2019/04 [Refereed]
- KOBAYASHI Toru; FUJII HikaruAquaculture Research 2019 [Refereed]
- Toru Kobayashi; Tomoki Honryo; Yasuo Agawa; Yoshifumi Sawada; Ileana Tapia; Karla A. Macias; Amado Cano; Vernon P. Scholey; Daniel Margulies; Naoki YagishitaREPRODUCTIVE BIOLOGY INST ANIMAL REPRODUCTION FOOD RESEARCH 15 (2) 106 - 112 1642-431X 2015/06 [Refereed]
To develop techniques for seedling production of yellowfin tuna, the behavior of primordial germ cells (PGCs) and gonadogenesis were examined at 1-30 days post hatching (dph) using morphometric analysis, histological examination, and in situ hybridization. Immediately after hatching, PGCs were located on the dorsal side of the posterior end of the rectum under the peritoneum of the larvae, and at 3 dph they came into contact with stromal cells. PGCs and stromal cells gradually moved forward from the anus prior to 5 dph. At 7-10 dph, germ cells were surrounded by stromal cells and the gonadal primordia were formed. In individuals collected at 12 dph, PGCs were detected by in situ hybridization using a vasa mRNA probe that is a germ-cell-specific detection marker. The proliferation of germ cells in the gonadal primordia began at 7-10 dph. We observed double the number of germ cells at 30 dph (22 +/- 3.2 cells), compared to that at 1 dph (11 +/- 2.1 cells). Therefore, based on our data and previous reports, the initial germ cell proliferation of yellowfin tuna is relatively slower than that of other fish species. (C) 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All rights reserved. - Yasuo Agawa; Mayui Iwaki; Takafumi Komiya; Tomoki Honryo; Kouhei Tamura; Tsukasa Okada; Naoki Yagishita; Toru Kobayashi; Yoshifumi SawadaFISHERIES SCIENCE SPRINGER JAPAN KK 81 (1) 113 - 121 0919-9268 2015/01 [Refereed]
A male-specific marker of a DNA sequence for the Pacific bluefin tuna Thunnus orientalis, Male delta 6 (Md6), was identified by comparative study of the sequences obtained by F3-cultured male and female AFLP-selective DNA amplification products, followed by high-throughput DNA sequencing. Md6 was characteristic in continuous 6-bp nucleotide deletions compared to redundant sequences that could be seen in both sexes. The PCR genotyping primers were developed based on the Md6 sequence, which showed that 29 of 32 F3 males (90.6 %) and 9 of 13 (69.2 %) F2 parental males were genotyping positive, whereas in females, the genotyping test was negative in parental F2 (n = 12), and 1 of 32 (3.1 %) F3 female was Md6-positive. Parentage allocation tests suggested that Md6-positive F2 males were more attendant in spawning than Md6-negative males, suggesting that Md6 was passed from male parents to male progeny under aquaculture conditions. - Shohei Nomura; Toru Kobayashi; Yasuo Agawa; Daniel Margulies; Vernon Scholey; Yoshifumi Sawada; Naoki YagishitaFISHERIES SCIENCE SPRINGER JAPAN KK 80 (6) 1193 - 1204 0919-9268 2014/11 [Refereed]
The genetic population structure of the Pacific bluefin tuna (PBF) Thunnus orientalis and the yellowfin tuna (YFT) T. albacares in the North Pacific Ocean was investigated. The polymorphism of microsatellite (SSR) loci and sequences of mitochondrial DNA control region (mtCR) were analyzed for 71 samples of PBF from Japan and Mexico and 45 samples of YFT from Japan and Panama. In the SSR analyses, both single-locus (-0.010 to 0.008 in PBF and -0.023 to 0.020 in YFT) and global multilocus (0.003 in PBF and -0.002 in YFT) F-ST values among the geographic populations were low and not significant in these species. In the mtCR analyses, neither the neighbor-joining tree nor the minimum spanning network showed genetic differentiation among the geographic populations in each species. The pairwise F-ST values among the geographic populations of them (-0.005 in PBF and -0.020 to -0.014 in YFT) were low and not significant. Our SSR and mtCR data suggested that genetic differentiations were not evident among the eastern and western populations in the North Pacific Ocean either in PBF or in YFT. Mismatch distributions, demographic parameters, and neutrality tests suggested that sudden population expansion of PBF and YFT in the North Pacific Ocean occurred 628,000-731,000 and 450,000-525,000 years ago, respectively. - Guillen A.; 本領 智記; Ibarra J.; Cano A.; Margulies D.; Scholey V.P.; Wexler J.B.; Stein M.S.; 小林 徹; 澤田 好史水産増殖 水産増殖談話会 62 (3) 319 - 322 0371-4217 2014/09東太平洋キハダの卵内発育に及ぼす水温の影響
- GUILLEN ANGEL; HONRYO TOMOKI; IBARRA JUAN; CANO AMADO; MARGULIES DANIEL; SCHOLEY VERNON P; WEXLER JEANNE B; STEIN MARIA S; KOBAYASHI TORU; SAWADA YOSHIFUMIAquaculture Science 62 (3) 319 - 322 0371-4217 2014/09 [Refereed]
- Naoki Yagishita; Yoshifumi Sawada; Yasuo Agawa; Toru KobayashiCONSERVATION GENETICS RESOURCES SPRINGER 6 (1) 189 - 191 1877-7252 2014/03 [Refereed]
The pacific bluefin tuna (PBT) Thunnus orientalis is one of the most important species for fisheries in the world. This species has been highly exploited in fisheries, resulting in dwindling of its natural resources. The artificial hatching of a third generation of fully cultured PBT was a success in 2007, suggesting the possibility of mass production of PBT for food and for seed release. To enhance stock structure studies and investigate genetic feature of seedlings, we isolated 25 polymorphic microsatellite loci (2-18 alleles/locus; expected heterozygosity, 0.042-0.941) from PBT. - Hiroshi Ashida; Naoki Ueyama; Masato Kinoshita; Toru KobayashiREPRODUCTIVE BIOLOGY INST ANIMAL REPRODUCTION FOOD RESEARCH 13 (4) 317 - 324 1642-431X 2013/12 [Refereed]
Willow minnow, Gnathopogon caerulescens, is a commercially important freshwater fish species in Japan. In particular, female fish with eggs are more expensive than male fish. Many methods have been tested to achieve 100% willow minnow female production; however, none of them were successful because of the temperature-dependent sex determination mechanism. To date, the molecular mechanism underlying sex determination in willow minnow is still not completely understood. This study was designed as an attempt to understand the sex differentiation mechanism of willow minnow at the molecular level. We cloned the mRNAs of two sex-related genes of G. caerulescens forkhead-box protein L2 (FOXL2) and doublesex- and mab-3-related transcription factor 1 (DMRT1). We compared the inferred amino acid sequences to those of other fish and examined the expression patterns of these genes in adult tissues and during development. The FOXL2 and DMRT1 genes of willow minnow were isolated and identified. Both gene sequences share high homology with those of Chinese Cyprinidae. The cell- and tissue-specific expression of FOXL2 and DMRT1 were also determined. Both genes were expressed in gonads, and their expression level increased before gonadal sex differentiation. Overall, these results suggest that these two genes play a key role in the sex differentiation of willow minnow. Detailed knowledge regarding this mechanism will be useful for efficient all-female cyprinid fish production that follows a temperature-dependent sex determination system. (C) 2013 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved. - 宮口修平; 田島灯台; 小林 徹; 柳下直己近大農紀要 近畿大学農学部 46 (46) 37 - 53 0453-8889 2013/03[Synopsis] The members of the Laboratory for Aquatic Biology, Faculty of Agriculture, Kinki University collected aquatic animals at Sakatahana, Shirahama-cho, Wakayama Pref. during field practice every summer from 2007 to 2011. Here, we made a provisional list of the collected specimens of fishes, crustaceans, gastropods, and bivalves that include 58, 37, 27, and 12 species, respectively. Among them, the numbers of the species of temperate to subtropical waters were 36 in fishes, 13 in gastropods, and 4 in bivalves.
- Yasuyuki Tsukamasa; Keisuke Kato; Bimol Chandra Roy; Yasunori Ishibashi; Toru Kobayashi; Tomohiro Itoh; Masashi AndoFisheries Science 79 (2) 349 - 355 0919-9268 2013 [Refereed]
The color of the red muscle of pelagic fish is used as an indicator of freshness, and sustaining a bright red color is important for maintaining the commercial value of pelagic fish. Feeding fish with antioxidant compounds can delay metmyoglobin (metMb) formation, but the process requires long-term feeding. Live cultured Japanese horse mackerel Trachurus japonicus were used in this study. After anesthetization, 2 ml of blood was drawn from blood vessels, and a parenteral solution including an antioxidant compound was injected. Browning, metMb formation, and lipid oxidation of dark muscle during chilled storage were delayed by injecting sodium l-ascorbate as an antioxidant compound in the blood vessels of live fish. © 2013 The Japanese Society of Fisheries Science. - Yasuo Agawa; Tomoki Honryo; Ayako Ishii; Toru Kobayashi; Hiromi Oku; Yoshifumi SawadaAQUACULTURE RESEARCH WILEY-BLACKWELL 43 (8) 1145 - 1158 1355-557X 2012/07 [Refereed]
Pacific bluefin tuna (PBT) peroxisome proliferator-activated receptor ? (PPAR?) gene was characterized to know the expression of its transcript contribution to the development of PBT, because PPAR? is the key molecule for adipose cell differentiation. Resulting cDNA and deduced amino acid sequence had high similarities to other teleosts that consisted of A to F domains. Phylogenetic tree analysis indicated a close relationship PBT PPAR? to cobia and flatfishes ones among the teleosts with the similarity of characteristic insertion sequences at amino terminal region of E/F domains. PPAR? transcripts quantification profile in the tissues of a 13-month-old PBT indicated the correlation between its quantity with the muscle types of different lipid content. The transcripts were also detected in the head kidney, digestive organs and red muscle with higher level inferred the PPAR? contribution to the multiple physiological processes. PPAR? transcripts were quantified for PBT juveniles fed artificial diets to investigate the effect of phospholipid supplementation on the growth, survival and PPAR? expression, to verify the efficacy of phospholipid source. No obvious difference in growth performance, survival and gene expression of PBT juveniles was identified, this may be due to DHA/EPA ratio analogy between diets and indicating that phospholipid sources in the juvenile diet might be adaptable. - Yoshio Kunimune; Yasushi Mitsunaga; Kazuyoshi Komeyama; Masanari Matsuda; Toru Kobayashi; Tsutomu Takagi; Takeshi YamaneFISHERIES SCIENCE SPRINGER TOKYO 77 (4) 521 - 532 0919-9268 2011/07 [Refereed]
Seasonal habitat use by nigorobuna Carassius auratus grandoculis Temminck et Schlegel and gengoroubuna Carassius cuvieri (Temminck et Schlegel) in Lake Biwa was investigated using acoustic telemetry. Twenty-three nigorobuna and 11 gengoroubuna specimens caught using set-nets in the lake's south basin were surgically fitted with acoustic transmitters and then released. Signals from the fishes were recorded by 23 receivers installed around the lake. Between April and June 2007, the first spawning season after release, signals were received from all tagged fishes. Thereafter, until the second spawning season, signals were collected from 26 and 45% of released nigorobuna and gengoroubuna individuals, respectively. Seasonal habitat preferences for these species were studied by distance-based analysis. The analysis revealed that nigorobuna tended to stay near their spawning area in the south basin of the lake throughout the year, whilst gengoroubuna tended to show a seasonal migration pattern between the north and south basins. After the spawning season, the latter species migrated to the north basin where it remained until the next spawning season, when it returned to the south basin. This is the first report of seasonal migration of nigorobuna and gengoroubuna in Lake Biwa. - Toru Kobayashi; Ryo Ishibashi; Shinji Yamamoto; Satoshi Otani; Koichi Ueno; Osamu MurataAQUACULTURE RESEARCH WILEY-BLACKWELL PUBLISHING, INC 42 (2) 230 - 239 1355-557X 2011/01 [Refereed]
The timing of primordial germ-cell (PGC) migration with regard to the gonadal anlagen, gonad formation and sex differentiation was examined histologically in the chub mackerel (Scomber japonicus) at 5-190 days post hatching (dph). At 5 dph, PGCs appeared on the peritoneal epithelium surface or in the mesentery, on the dorsal side of the abdominal cavity. By 10 dph, stromal cells around the PGCs proliferated. The gonadal primordium was formed by 15 dph. The gonadosomatic index was 0.01% at 30 dph and increased thereafter (0.32% in females and 0.04% in males at 160 dph). Ovarian differentiation occurred at 30-40 dph, indicated by ovarian cavity formation (elongation and fusion of the upper and lower ovarian edges). Meiosis was subsequently initiated. A few meiotic oocytes surrounded the cavity at 50 dph; most were in the perinucleolus stage at 60 dph and attained a diameter of 60-70 mu m at 190 dph. Testicular differentiation occurred at 30 dph, indicated by the formation of the sperm duct primordium. Spermatogonia gradually proliferated, developing into spermatocytes at the chromatin-nucleolus stage (after 90 dph) and subsequently into spermatids and spermatozoa (160 dph). These data could aid the development of seeding and cell-engineering technologies for scombrid fish. - KOBAYASHI TORU; FUSHIKI SHOZOAquaculture Science 水産増殖談話会 59 (2) 191 - 198 0371-4217 2011 [Refereed]
醒井養鱒場ニジマス血統集団から選抜された個体を用いた雌性発生継代によって、早期産卵形質および大型卵産出形質に関する遺伝的固定をおこなった。夜間照明による成熟調節操作を受けた1986年夏季産卵群(P)の再生産形質のうち排卵時期と産出卵のサイズの分布を調べ、産卵期間のうちで早期、中期、晩期に排卵した個体の中から大型卵を産出した個体をそれぞれ選抜し、それらの卵を用いて雌性発生で継代した。雌性発生1代目(1G)は1988年の秋に成熟し、それぞれの次世代集団は各選抜親魚の産卵期および大型卵産出に関する形質をよく受け継いでいた。そのうち特に早期に大型卵を産出したC系統の個体を用いて雌性発生継代した2代目(2G)は醒井通常系統よりも6週間も早い9月20日から排卵を開始した。1Gの卵サイズは系統ごとに広くばらついた。雌性発生C系統の1代目の平均卵径は4.90mmとP群の1.06倍であり、その実現遺伝率は0.164であった。 - Kobayashi T; Nemoto MAquaculture Science 水産増殖談話会 58 (3) 337 - 343 0371-4217 2010 [Refereed]
ニゴロブナにおける雌性発生卵の第二極体放出阻止のための低温刺激処理(CST)および高温刺激処理(HST)後の染色体の行動を観察した。受精7日後の正常仔魚生存率はCSTおよびHSTをそれぞれ媒精後7分および9分に開始した場合に最大値を示した。雌性発生は1腹卵を使って紫外線照射精子(3,000erg/mm2)で誘導した。CSTの後、第二減数分裂の紡錘体が消失し、カリオメアと半数性核、あるいは二倍性核が、雌性前核と極体の代わりに観察された。これらの構造体は処理後15分(媒精後62分)に精子星状体の中心へ移動して接合核を形成し、媒精後72分に第1卵割が起こった。一方HST後では、卵割は処理後12分に起こった。核の変化はCST後よりもHST後の方が早かったが、両処理後の核の行動は類似していた。カリオメアおよび前核の行動の違いは処理の違いではなく、卵の熟度と感受性の違いによっていると考えられた。 - Kohsuke Adachi; Keitaro Kato; Masayoshi Yamamoto; Katsuya Ishimaru; Toru Kobayashi; Osamu Murata; Hidemi KumaiAQUACULTURE ELSEVIER SCIENCE BV 296 (1-2) 110 - 116 0044-8486 2009/11 [Refereed]
We cloned the prolactin (PRL) and somatolactin (SL) genes of Pacific bluefin tuna (PBT: Thunnus orientalis) and investigated their daily mRNA expression patterns under aquacultured conditions. The PRL and SL cDNAs of PBT encode 211 and 231 aa prehormones which give rise to 187 and 207 aa mature peptides, respectively, and which belong to the same clades as those of other Perciformes. The highest gene expression levels of PRL and SL in PBT were found at 6 am under natural light conditions, which is about 3 times higher than the constitutive levels in both cases. Interestingly, scatter diagrams show a strictly correlated expression between PRL and SL (r=0.82: p<0.01) while no correlation was found between PRL or SL and the growth hormone (GH). These results suggest that PRL and SL play synchronized role(s) in daily physiological phases of PBT under aquacultured conditions. (C) 2009 Elsevier B.V. All rights reserved. - T. Kobayashi; Shozo Fushiki; Koichi UenoAquaculture Science 57 (3) 361 - 370 0371-4217 2009 [Refereed]
- Kohsuke Adachi; Keitaro Kato; Masayoshi Yamamoto; Katsuya Ishimaru; Toru Kobayashi; Osamu Murata; Hidemi KumaiAQUACULTURE ELSEVIER SCIENCE BV 281 (1-4) 158 - 161 0044-8486 2008/09 [Refereed]
The daily expression profile of growth hormone (GH) mRNA in juvenile Pacific bluefin tuna (Thunnus orientalis) was investigated under aquacultured conditions. Total RNA from pituitaries (n=5) was sampled from 10 am to 12 pm the next day at 1 h intervals. The expression levels of GH mRNA were evaluated using real-time PCR normalized against the beta-actin gene. The expression level of GH transcripts reached a peak at 3-4 am at which time it was about 10 times higher than at any other time period. Considering their high growth rate compared to red seabream (Pagrus major) and other aquacultured species which shows continuous CH mRNA expression patterns, it can be concluded that the pulsed expression of GH mRNA just before daybreak is a key for the extremely high somatic growth rate of Pacific bluefin tuna. (C) 2008 Elsevier B.V. All rights reserved. - Takashi Suzuki; Toru Kobayashi; Koichi UenoENVIRONMENTAL BIOLOGY OF FISHES SPRINGER 82 (4) 353 - 364 0378-1909 2008/08 [Refereed]
To understand the differences in the spawning sites among Cyprininae fishes in Lake Biwa, we conducted periodic sampling of larvae and juveniles at three sites (irrigation ditch, St. 1; river, St. 2; and satellite lake, St. 3). On the basis of species/subspecies identification by using RAPD analysis, we examined the species composition of the larvae and juveniles at these three sampling sites. The number of specimens was 616, 68, and 117 at St. 1, St. 2, and St. 3, respectively. Based on morphological and genetic identification, the specimens were found to include nine fish species/subspecies, namely, Carassius auratus grandoculis, Carassius cuvieri, Carassius auratus langsdorfii, Cyprinus carpio, Sarcocheilichthys sp., Silurus asotus, Oryzias latipes, Odontobutis obscura obscura, and Rhinogobius sp. The species composition at the three sites also differed. Among the Cyprininae fishes, C. auratus grandoculis, C. auratus langsdorfii, and Cyprinus carpio were found in abundance at St. 1; C. cuvieri was not collected from St. 1 but was found at the other two sites, particularly St. 3. Among the other fishes, Rhinogobius sp. was collected at St. 1 and St. 3, whereas the other four occurred only at St. 1. These results suggest that the selection of spawning sites by C. cuvieri differs to a certain extent from that of the other Cyprininae fishes, and the irrigation ditch in the lake is an important habitat for the larvae and juveniles of native fish species. - N. Yagishita; T. KobayashiMOLECULAR ECOLOGY RESOURCES BLACKWELL PUBLISHING 8 (2) 302 - 304 1471-8278 2008/03 [Refereed]
The stock abundance of the chub mackerel Scomber japonicus - a very important species for fisheries, particularly in Japan - in the Pacific Ocean off Japan has remained at a low level. For studying the population genetics of the chub mackerel, we isolated nine polymorphic microsatellite loci (12-31 alleles/locus; expected heterozygosity, 0.762-0.983) from this species. Cross-species amplification indicated that eight of the nine microsatellite loci in the blue mackerel S. australasicus were polymorphic and functional. - Satoshi Otani; Manabu Ohara; Shigeru Miyashita; Toru KobayashiFISHERIES SCIENCE BLACKWELL PUBLISHING 74 (1) 208 - 210 0919-9268 2008/02 [Refereed]
- Ishibashi R; Yamamoto S; Kobayashi T; Izumi K; Miyashita S; Murata OAquaculture Science Japanese Society for Aquaculture Science 55 (1) 17 - 22 0371-4217 2007 [Refereed]
Human chorionic gonadotropin (HCG) was injected to one-year old captive chub mackerel (Scomber japonicus) in June 2005, a timing equivalent to the fish spawning period, to see if fertilized eggs would be formed. The sample fish were accommodated in a net cage and measured for total length, fork length, body length, head length, body depth, body width and body weight every 10 days until 100 days after hatching, then every 30-day thereafter. The sample fish were also checked for ovulation and spermiation by abdominal thrust 280 days after hatching. Their gonadal gland was then removed, weighed and measured for gonad somatic index (GSI) and, for females, KG value. Although spermiation was not confirmed in any males 280 days in age, spermiation was confirmed in half the male fish after 310 days in age and in all the male fish after 340 days in age. For the female fish, no ovulation was observed in individuals 310 days in age, but ovulation was confirmed in 8 out of 10 fish 340 days in age. Five groups of 10 male and 10 female in each received single injection of HCG (200 IU/kg BW) from 360 days in age (1st group) to 376 days in age (5th group) . The fish in four group spawned by the 3 days after the injection. In the group of eggs injected on June 27 (4th group), fertilized eggs were seen, although in a small quantity. When these fertilized eggs were cultured, they were observed to hatch normally and the hatchlings grew successfully. - T Suzuki; H Nagano; T Kobayashi; K UenoFISHERIES SCIENCE JAPANESE SOC FISHERIES SCIENCE 71 (3) 679 - 681 0919-9268 2005/06 [Refereed]
- T Suzuki; H Nagano; T Kobayashi; K UenoNIPPON SUISAN GAKKAISHI JAPANESE SOC FISHERIES SCIENCE 71 (1) 10 - 15 0021-5392 2005/01 [Refereed]
We investigated species composition, distribution and seasonal changes in the number of larvae and juveniles of crucian carps in the reed zone of Lake Biwa using RAPD markers. A total of 973 larvae and juveniles were collected from the reed zone in a total of nine samplings conducted from April to July, 2002. In the first sampling, 868 larvae and juveniles, mainly preflexion larvae, were collected, and it was found that Gengoroubuna and Ginbuna are distributed within the reed zone. However, Nigorobuna distributed between the center and close to the shore within the reed zone. After the first sampling, larvae and juveniles, mainly postflexion larvae, were collected close to the shore in the reed zone, and most of those were Nigorobuna. - T Kobayashi; S Fushiki; K UenoENVIRONMENTAL BIOLOGY OF FISHES SPRINGER 69 (1-4) 419 - 425 0378-1909 2004/03 [Refereed]
Changes in the motility time of spermatozoa collected from the testes and the sperm duct of normal and sex-reversed male (XX) rainbow trout in physiological balanced salt solution were examined after incubation in artificial seminal plasmas of various pHs. Although untreated spermatozoa from the sperm duct retained motility for 60-90 s in the balanced salt solution, the spermatozoa collected from the testes were immotile. During the incubation in artificial seminal plasma of pH 7.0, the spermatozoa from the sperm duct hardly moved, similar to the testicular spermatozoa in the balanced salt solution. By suspending and incubating the testicular spermatozoa in artificial seminal plasma of pH 9.9 for 2 h at 4degreesC, the percentage of motile spermatozoa increased from 0-5% to 80%. The spermatozoa remained motile for at least 2 min after long-term incubation (12 h). When the full-sib eggs were inseminated with untreated testicular spermatozoa or testicular sperm treated for 2 h at high pH, the percentage survival increased from 5.5% to 53.8% at the eyed stage due to the high-pH treatment. The incubation of the spermatozoa in high-pH artificial seminal plasma improved the motility of the spermatozoa from the testes of the sex-reversed male that had lost its sperm duct. By this treatment, it is possible to markedly increase the mass production efficiency of all-female or all-female triploid sterile progenies. - M Nakagawa; T Kobayashi; K UenoJOURNAL OF EXPERIMENTAL ZOOLOGY WILEY-LISS 293 (6) 624 - 631 0022-104X 2002/11 [Refereed]
As part of the technique for preservation of endangered fish species, germline chimeras using the loach (Misgurnus anguillicaudatus) model were generated by embryonic cell manipulation. Transplanting cells from early-stage embryos of a wild-type strain to the blastoderm of an orange-type strain generated chimeras. Within three days, many of the resulting individuals exhibited a dark pigment on several parts of their body, characteristic of the donor but not of the orange strain. At one year of age, four chimeras (one female and three males) were pair-mated with the orange type, and F, was obtained. One of the four F-0 chimeras produced progeny similar to the wild type at a frequency of 0.24% (2/841). The results suggest that this method is a promising technique for the preservation of endangered fish species. (C) 2002 Wiley-Liss, Inc. - K Ueno; K Ota; T KobayashiGENETICA KLUWER ACADEMIC PUBL 111 (1-3) 133 - 142 0016-6707 2001 [Refereed]
The karyotype and DNA content of four lizardfish species (family Synodontidae), that is, Saurida elongata, Synodus ulae, Synodus hoshinonis and Trachinocephalus myops, were analyzed. The karyotype of T. myops significantly differed from that of the other three species having diploid chromosome number of 48 with mainly acrocentric chromosomes and the ZZ-ZW sex chromosome system. The chromosome number of male T. myops was 2n = 26, while that of female T. myops was 2n = 27. The karyotype consisted of 11 pairs of metacentrics, one pair of acrocentrics and, in addition, two large metacentrics in the male and a single large metacentric, a distinctly small subtelocentric and a microchromosome in the female. C-banding demonstrated that in the female the subtelocentric chromosome and the microchromosome were heterochromatic. The karyotype of T. myops was thought to be derived from a 48 chromosome type synodontid fish through the involvement of Robertsonian rearrangement; the rearrangement of the sex chromosomes proceeded during karyotype evolution. Among the chromosomes, the large metacentrics were determined to be neo-Z (a fusion of the original Z and an autosome), the microchromosomes the W-1 (originally W), and the subtelocentric chromosomes the W-2 (derived from an autosome pair). The miniaturization of W-1 and W-2 chromosomes and their heterochromatinization suggested that sex chromosomes in this species have been already highly differentiated. The findings on DNA content implied that the karyotype of T. myops evolved by centric fusion events without loss in DNA amount. - Effect on Crowding of Water Fleas, MIJINKO, with verious wavelengthlights.KOBAYASHI ToruReserch Report of Shiga Prefecture Fisheries Experimental Station 48 27 - 31 2001 [Refereed][Invited]
- K Ota; T Kobayashi; K Ueno; T GojoboriGENE ELSEVIER SCIENCE BV 259 (1-2) 25 - 30 0378-1119 2000/12 [Refereed]
The fish order Aulopiformes contains both synchronously hermaphroditic and gonochoristic species. From the cytogenetic viewpoint, few reports show that gonochoristic Aulopiformes have heteromorphic sex chromosomes. Because fish in this order give us a unique opportunity to elucidate the evolution of sex chromosomes, it is important to examine a phylogenetic relationship in Aulopiformes by both molecular evolutionary and cytogenetic methods. Thus, we conducted molecular phylogenetic and cytogenetic studies of six Aulopiform species. Our results suggested that hermaphroditic species were evolutionarily derived from gonochoristic species. It follows that the hermaphroditic species might have lost the heteromorphic sex chromosomes during evolution. Here, we suggest a possibility that heteromorphic sex chromosomes can disappear from the genome. even if they have appealed once in evolution. Taking into account Ohno's hypothesis that heteromorphic sex chromosomes might have emerged from autosomes. we propose the hypothesis that heteromorphic sex chromosomes may have undergone repeated events of appearance and disappearance during the course of fish evolution. (C) 2000 Elsevier Science B.V. All rights reserved. - T Kobayashi; K UenoNIPPON SUISAN GAKKAISHI JAPANESE SOC FISHERIES SCIENCE 66 (5) 840 - 845 0021-5392 2000/09 [Refereed]
Behavior of spindle and chromosomes in early development of gynogenetic amago salmon eggs was examined for explication of basic cytological mechanisms to induce gynogenetic diploid using genetically inactivated rainbow trout sperm by W-irradiation and with heat shock treatment (0 degreesC for 20 min) for retention of second polar body. The heat shock treatment that started 30 minutes after insemination of amago salmon eggs incubated at 12.0 degreesC was the optimum condition to induce meiotic gynogenetic diploids compared with other conditions of treatment. At 30 minutes, the eggs were in late anaphase of meiosis II. The percentage of eggs that became diploid larvae when treatment started at 5 to 30 minutes after insemination varied little. When treatment started at anaphase, the survival rate was greater than when treatment started at metaphase. It seems that physical damage to the eggs caused by heat is less at anaphase. The second polar body was retained in fewer eggs when heat treatment started later, in the range of 35 to 60 minutes after insemination. Cytokinesis may have prevented such retention more than the increased distance between chromosomes. - T KobayashiNIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES SCI 64 (5) 782 - 791 0021-5392 1998/09 [Refereed]
Heat shock treatment for 20 min from 15 min after insemination suppressed the formation of the second polar body of the normally developed eggs, and the genetic material of the second polar body was retained in the egg. These processes did not result in the formation of a diploidy female pronucleus, but formed some karyomeres. They were tugged with the female pronucleus toward the male pronucleus already present in the center of the sperm aster. Thus, they adhered, and formed a fertilized nucleic group like a cluster of grapes. The genetic inactivated sperm irradiated with ultraviolet rays (UV-sperm) became swollen and formed a male pronucleus after passing through the micropile. It behaved like normal sperm until the connection with the female pronucleus. The paternal genetic material in gynogenetic embryos disappeared during the anaphase of the first cleavage, caused by the incomplete chromosome formation from the male pronucleus, and the paternal DNA remained near the cleavage plane. In the gynogenetic diploid embryo inseminated with UV-sperm, and polyploidized with heat shock treatment, the above mentioned cytological events caused by dual artificial manipulation occurred in the same egg, i.e., the genetic material formed a triploidy nucleus like a cluster of grapes, and a male pronucleus derived from W-sperm disappeared during the anaphase of the first cleavage. The developmental rate was higher in triploid embryos polyploidized with heat shock, and lower in the gynogenetic haploid embryo than in the normal diploid embryos. In the gynogenetic diploid embryos, the metaphase of the first cleavage occurred at the same time as normal diploid embryos. - T Kobayashi; S Fushiki; N Sakai; A Hara; M Amano; K Aida; M Nakamura; Y NagahamaFISHERIES SCIENCE JAPAN SOC SCI FISHERIES SCI 64 (2) 206 - 215 0919-9268 1998/04 [Refereed]
The reproduction of artificially induced triploid rainbow trout was histologically and endocrinologically examined during three consecutive seasons. The gonadosomatic indices of triploid females were much lower (0.02-0.06) than the indices of diploid females. The concentrations of sex steroid hormones, including testosterone, estradiol-17 beta, and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one, were much lower than those of diploid females but had small changes, and changes of gonadotropin (GtH) levels in the serum of triploid females differed from those of diploid females. Oogenesis in the triploid ovary was substantially delayed, and most germ cells ceased to develop at the synaptic stage of the first meiotic division. At three years old, various developmental stages of oocytes were detected in the ovary, such as peri-nucleolus, yolk vesicle, and early yolk globule stage. It seemed that these rare developments of triploid oocytes were related to the small increases of steroid hormone concentrations and rise of GtH level that appeared when fish were two years old and three years old. Nevertheless, even in triploid ovarian oocytes grown to secondary yolk globule stage, the germinal vesicles did not migrate and there was no ovulation. It was confirmed that triploid liver cells possessed the potential to produce vitellogenin. These results suggest that the genital gland of triploid females is undeveloped because the odd number of chromosome sets causes the first maturation division to fail, and the endocrinological abnormality. - Toru Kobayashi; Seiji Ohno; Takanori KobayashiDNA多型 6 219 - 226 1998 [Refereed]
- 小林 徹海洋 海洋出版 号外14 (14) 206 - 210 0916-8575 1998
- Toru Kobayashi; Shozo FushikiSUISANZOSHOKU 45 (1) 87 - 96 0371-4217 1997/03 [Refereed]
- T KobayashiFISHERIES SCIENCE JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 63 (1) 33 - 36 0919-9268 1997/02 [Refereed]
The artificial induction of gynogenesis of amago salmon was cytologically confirmed, and the nucleus behavior and the cytological rates were compared at the early stage of development among the following three groups: normal, hybrid between the female amago salmon and the male rainbow trout, and gynogenetic developed group. The first cleavage of hybrid and gynogenetic development was delayed compared with normal development, while that in the gynogens was later than that of the hybrid. The cytological stage at 68 h. degrees C in cumulative temperature, which is considered the optimum timing for suppressing the first cleavage of gynogen, was prometaphase. In gynogenetically activated eggs, the lump-shaped body appeared in the metaphase plate. Then, it remained near the cleavage plane in the following cell division, and was considered to be degenerated nuclear materials derived from irradiated sperm, i.e. dense chromatin body (DCB), which could not transform to chromosomes. Therefore, paternally derived genetic materials should disappear during the anaphase and the telophase in the first cleavage of gynogenetic development. Some chromosomes were gradually eliminated in the hybrid development after the first cleavage. This phenomenon was considered a major cause of inviability and the subsequent expression of abnormality during the development of the hybrid. - 小林 徹東京大学農学生命科学研究科審査博士学位論文 PP 304 - 230 1997
- Toru Kobayashi; Katsutoshi Hori; Shozo Fushiki; Soichi Nakamura; Koichi UenoSUISANZOSHOKU 水産増殖談話会 43 (3) 395 - 400 0371-4217 1995/09 [Refereed]
- Kohichi Fujiwara; Takahiro Usui; Toru Kobayashi; Eiji MizutaniKankyo System Kenkyuu 23 414 - 419 1995 [Refereed]
- T KOBAYASHI; A IDE; T HIASA; S FUSHIKI; K UENOFISHERIES SCIENCE JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 60 (3) 275 - 281 0919-9268 1994/06 [Refereed]
We induced homozygous clones of amago salmon by gynogenetic chromosome manipulation. Mitotic gynogenesis diploid (mitotic-G2N) as first generation was produced by suppression of the first cleavage of the eggs inseminated with UV-irradiated sperm of rainbow trout by hydrostatic pressure shock. The yield of apparently normal gynogenetic embryos was 2.26% when the eggs were shocked at 650 kg/cm2 of hydrostatic pressure for 6 min at 5 h 30 min (approximately 68 h . degrees-C in cumulative temperature) after insemination. Homozygous evidence of gynogenetic juveniles was demonstrated by the segregation of Idh-3 and Pgm-1 loci. Five homozygous inbred strains were produced from the eggs of homozygous gynogenetic females by retaining the second polarbody, while outbred controls were produced for each strain by crossing with males of other strains. The clonal proof of these strains was demonstrated by the immunological acceptance of operculum allografts from inbred sisters. Similar survivals were shown among clones produced by mothers from identical strain. On the other hand, various survivals were seen between the clones using maternal parents from different strains. These results suggested that the line selection was a vital factor in clone production. - T KOBAYASHI; N SAKAI; S FUSHIKI; Y NAGAHAMA; M AMANO; K AIDANIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 59 (6) 981 - 989 0021-5392 1993/06 [Refereed]
Testicular development and hormone levels of triploid males of rainbow trout were compared with those of diploid males during three consecutive seasons. Changes in the levels of serum sex steroid hormones (testosterone, 11-ketotestosterone, and 17alpha,20beta-dihydroxy-4-pregnen-3-one) and pituitary and serum gonadotropin in triploids were similar to those in diploids. Although meiotic division first occurred in the July testes of both triploids and diploids, subsequent spermatogenesis was markedly delayed in the testes of triploids. During the natural spermiating season, a large number of atretic figures of spermatogenic germ cells were found in the testes of triploids with a sudden decrease in the gonadosomatic index. The amount of sperm was markedly less in triploids than in diploids. Some necrotic cells were invested by hypertrophied Sertoli cells lining the inner wall of the lobular lumen. Taken together, these results suggest that the cause of abnormality of spermatogenesis in triploids is the disruption of meiosis due to the odd number of chromosome sets. - T Yada; T Kobayashi; A Urano; T HiranoJOURNAL OF EXPERIMENTAL ZOOLOGY WILEY-LISS 262 (4) 420 - 425 0022-104X 1992/07 [Refereed]
To examine the changes in secretion of growth hormone (GH) and prolactin (PRL) with reference to their osmoregulatory roles, changes in pituitary mRNA levels and plasma concentrations of these hormones were examined during seawater adaptation in silvery juveniles (smolts) and precociously mature mates (dark parr) of amago salmon (Oncorhynchus rhodurus). Transfer to seawater increased plasma sodium levels in both smolts and dark parr. Smolts adjusted their plasma sodium to the level associated with seawater-adaptation (165 mEq/liter) within 3 days, whereas no adjustment was seen in dark parr; the latter failed to survive in seawater for more than 3 days. In smolts, plasma GH levels increased significantly 1 day after transfer, whereas there was no significant change in dark parr. An increase in GH mRNA levels was observed in smolts in association with increased plasma GH, whereas there was no change in dark parr. In contrast, a reduction in plasma PRL levels was consistently observed in both smolts and dark parr after transfer to seawater. However, there was no significant change in PRL mRNA levels in either smolts or dark parr. These results suggest that both gene expression and release of GH are activated by seawater transfer only in smolts with adequate seawater adaptability, whereas PRL gene expression is decreased after seawater transfer regardless of seawater adaptability. - Toru KobayashiSUISANZOSHOKU 40 (1) 57 - 70 0371-4217 1992/03 [Refereed]
- 小林 徹水産育種 17 1 - 4 1991
MISC
- 完全養殖クロマグロの遺伝的多様性.柳下直己; 小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 473 -474 2013
- 飼育水温がホンモロコ性分化関連遺伝子に及ぼす影響.芦田裕史; 木下政人; 小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 391 -392 2013
- クロマグロ若齢期における成長とIGF-1遺伝子発現.佐伯 康; 岡田貴彦; 本領智記; 阿川泰夫; 澤田好史; 柳下直己; 小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 388 -390 2013
- マダイにおける生殖巣形成過程.小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2009(平成20-21)年度中間成果報告書 250 2010
- マダイPagrus majorの初期発生過程における形態形成と始原生殖細胞の動態.小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2009(平成20-21)年度中間成果報告書 248 -249 2010
- ホンモロコGnathopogon caerulescensを用いた核移植クローン作出技術の開発試行.小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2009(平成20-21)年度中間成果報告書 246 -247 2010
- 養殖クロマグロThunnus orientalisの遺伝的多様性と家系判別.小林 徹; 阿川泰夫; 澤田好史 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2009(平成20-21)年度中間成果報告書 145 -146 2010
- Developmental biology and genetics in aquaculture.Kobayashi T; Ueno K; Yagishita N; Otani S The 21st Century COE Program Kinki University "Center of Aquaculture Science and Technology for Bluefin Tuna and Other Cultivated Fish" Final report: 2003-2007. 31 -37 2008
- KOBAYASHI TORU; UENO KOICHI; YAGISITA NAOKI; OTANI SATOSHI Final Rep 2003-2007 Cent Aquac Sci Technol Bluefin Tuna Other Cultiv Fish 21st Century COE Program Kinki Univ 31 -37 2008
- KOBAYASHI Toru Program Abstr 5th World Fish Congr 2008 367 2008
- Construction of a BAC library for the Pacific bluefin tuna Thunnus orientalis.Yagishita N; Oohara I; Kobayashi T Ecology and Aquaculture of Bluefin Tuna (Proceedings of the Joint international symposium on Bluefin Tuna, 2006 85 -88 2007
- Gonadal formation and development in cultured chub mackerel, Scomber japonicus.Kobayashi T; Ishibashi R; Yamamoto S; Ueno K; Murata O Ecology and Aquaculture of Bluefin Tuna (Proceedings of the Joint international symposium on Bluefin Tuna, 2006. 79 -84 2007
- T Kobayashi; M Nemoto; K Ueno AQUACULTURE 247- (1-4) 20 -20 2005/06
- クロマグロの卵割期発生過程.小林 徹; 大谷 哲; 宮下 盛 21世紀COEプログラム クロマグロ等の魚類養殖産業支援型研究拠点 2003-2004(平成15-16)年度中間成果報告書 35 -38 2005
- ホンモロコを用いた始原生殖細胞の移植技法開発.大谷 哲; 大原 学; 小林 徹 21世紀COEプログラム クロマグロ等の魚類養殖産業支援型研究拠点2003-2004(平成15-16)年度中間成果報告書 31 -34 2005
- 借腹養殖技法開発を目的としたマダイの胚発生に関する基礎的研究.小林 徹; 長谷川 徹; 家戸敬太郎; 大谷 哲 21世紀COEプログラム クロマグロ等の魚類養殖産業支援型研究拠点 2003-2004(平成15-16)年度中間成果報告書 23 -26 2005
- 借腹レシピエントとしてのマサバの生殖巣分化について.小林 徹; 石橋 亮; 大谷 哲; 村田 修 21世紀COEプログラム クロマグロ等の魚類養殖産業支援型研究拠点.2003-2004(平成15-16)年度中間成果報告書 19 -22 2005 [Refereed]
- AFLP法によるニジマスクローン作出の成否の検討亀甲武志; 小林 徹; 中山耕至; 甲斐嘉晃; 井戸本純一 平成15年度滋賀県水産試験場事業報告 307 -308 2004
- 琵琶湖南湖産イケチョウガイの遺伝的近交度の推定西森克浩; 小林 徹 平成12年度滋賀県水産試験場事業報告 22 -23 2001
- Effect on Crowding of Water Fleas, MIJINKO, with Various Wavelength LightsScience Report of Shiga Prefectural Fisheries Experimental Station 48- 27 -31 2001
- 上野紘一; 太田欽也; 小林徹 日本水産学会大会講演要旨集 1999- 70 1999/09
- 鈴木誉士; 小林徹; 小林敬典; 上野紘一 日本水産学会大会講演要旨集 1999- 1999
- 太田欽也; 上野紘一; 小林徹 日本水産学会大会講演要旨集 1998- 80 1998/09
- OTA KIN'YA; UENO KOICHI; KOBAYASHI TOORU 日本水産学会大会講演要旨集 1998- 95 1998/04
- 臼杵崇広; 小林 徹; 藤原公一 平成9年度滋賀県水産試験場事業報告 16 -17 1998
- 小林徹; 小林敬典 日本水産学会大会講演要旨集 1997- 1997
- 小林徹; 小林敬典 滋賀県水産試験場事業報告 1996- 45 -46 1997
- ニゴロブナにおける放流魚と天然魚の遺伝的変異性の比較小林 徹; 小林敬典 平成8年度滋賀県水産試験場事業報告 43 -44 1997
- ニゴロブナおよびホンモロコのクローン作出とその問題点小林 徹 平成8年度滋賀県水産試験場事業報告 41 -42 1997
- ヨシ・キシュウスズメノヒエ群落の状況追跡調査臼杵崇広; 小林 徹; 藤原公一; 的場 洋; 水谷英志 平成6年の異常渇水および湖水位低下による水産生物への影響調査報告書,滋賀県水産試験場刊 73 -106 1997
- ニゴロブナの0+~1+養成と飼料に対するタンパク要求量小林 徹 平成7年度滋賀県水産試験場事業報告 46 -47 1996
- 異なる3条件で飼育したニゴロブナの生残,成長および外部形態の比較根本守仁; 小林 徹 平成7年度滋賀県水産試験場事業報告 38 -39 1996
- 0+齢における全雌ニゴロブナの生物学的特性根本守仁; 小林 徹 平成7年度滋賀県水産試験場事業報告 36 -37 1996
- セタシジミのDNA分析手法の検討井戸本純一; 小林 徹 平成7年度滋賀県水産試験場事業報告 30 -31 1996
- 天然水域における餌料プランクトンの電照蝟集操作小林 徹; 臼杵崇広; 根本守仁 平成7年度滋賀県水産試験場事業報告 22 -23 1996
- ニゴロブナの遺伝的多様性検出のためのDNA増幅の試み小林 徹; 井戸本純一 平成7年度滋賀県水産試験場事業報告 20 -21 1996
- 臼杵崇広; 小林 徹; 藤原公一; 的場 洋 平成7年度滋賀県水産試験場事業報告 10 -11 1996
- KOBAYASHI TOORU; KUWAMURA KUNIHIKO 地域バイオテクノロジー実用化技術研究開発促進事業(水産業関係) 平成7年度成果概要 平成3-7年度成果概要 153 -155 1996
- 飼料への海藻粉末の添加がニゴロブナの成長に及ぼす影響小林 徹 平成6年度滋賀県水産試験場事業報告 1995
- ヨシ群落およびスズメノヒエ群落の水質・餌料環境臼杵崇広; 藤原公一; 小林 徹 平成6年度滋賀県水産試験場事業報告 14 -15 1995
- スズメノヒエ群落造成方法の検討臼杵崇広; 小林 徹; 藤原公一; 水谷英志 平成6年度滋賀県水産試験場事業報告 12 -13 1995
- 照射蛍光灯の波長光の違いによるミジンコ蝟集効果の差異小林 徹 平成5年度滋賀県水産試験場事業報告 22 -23 1994
- 染色体操作によるニジマス・アマゴの改良特性の検討ならびに優良形質固定等の育種に関する研究-Ⅱ小林 徹; 山中 治; 中 賢治; 平塚忠征 水産庁地域バイオテクノロジー研究開発促進事業報告書 27pp 1993
- 雌性発生超肥育系ニジマス三代目の作出小林 徹 平成4年度滋賀県醒井養鱒場業務報告 58 -61 1993
- パー系・スモルト系雌性発生アマゴ第二代の再生産関連形質小林 徹 平成4年度滋賀県醒井養鱒場業務報告 54 -57 1993
- 雌性発生ニジマス卵の第一卵割時期の細胞学的観察小林 徹 平成4年度滋賀県醒井養鱒場業務報告 49 -53 1993
- スモルト系クローンアマゴおよびクローンニジマスと,その交配対照区との成長変異の比較小林 徹 平成4年度滋賀県醒井養鱒場業務報告 44 -48 1993
- 摂餌競合激化環境状態における二倍体および三倍体ニジマスの摂餌量の差異小林 徹 平成4年度滋賀県醒井養鱒場業務報告 40 -43 1993
- 三倍体ニジマスにおける過激運動強制によるダメージからの回復速度小林 徹 平成4年度滋賀県醒井養鱒場業務報告 35 -39 1993
- 染色体操作によるニジマス・アマゴの改良特性の検討ならびに優良形質固定等の育種に関する研究-Ⅰ小林 徹; 大江孝二; 伊東正夫; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 12pp 1992
- パー系・スモルト系雌性発生アマゴ第二代の相分化小林 徹 平成3年度滋賀県醒井養鱒場業務報告 41 -44 1992
- 早期産卵大卵系雌性発生ニジマス二代目の産卵期小林 徹 平成3年度滋賀県醒井養鱒場業務報告 39 -40 1992
- 強制的過激運動下における三倍体ニジマスの血液性状の変化小林 徹 平成3年度滋賀県醒井養鱒場業務報告 35 -38 1992
- マス類の人為倍数体利用による育種に関する研究-Ⅴ小林 徹; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 41pp 1991
- クローンアマゴの量産の可能性小林 徹 平成2年度滋賀県醒井養鱒場業務報告 49 -52 1991
- 1989年産二代目雌性発生系統間での成長・体型及び抗病性の差異小林 徹 平成2年度滋賀県醒井養鱒場業務報告 46 -48 1991
- 雌性発生アマゴの成長変異とパー・スモルト変態小林 徹 平成2年度滋賀県醒井養鱒場業務報告 42 -45 1991
- 二倍体との摂餌競合が三倍体ニジマスの成長に及ぼす影響小林 徹 平成2年度滋賀県醒井養鱒場業務報告 36 -41 1991
- マス類の人為倍数体利用による育種に関する研究-Ⅳ小林 徹; 伊東正夫; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 27pp 1990
- アマゴのクローン作出について小林 徹 平成元年度滋賀県醒井養鱒場業務報告 56 1990
- 大型卵系,早期・晩期産卵系雌性発生二倍体第二代における体型について小林 徹 平成元年度滋賀県醒井養鱒場業務報告 52 -55 1990
- ニジマスにおける第一卵割阻止型雌性発生二倍体の同型接合性について小林 徹 平成元年度滋賀県醒井養鱒場業務報告 48 -51 1990
- 1+三倍体アマゴの2+年への生存性と寿命の延長小林 徹 平成元年度滋賀県醒井養鱒場業務報告 45 -47 1990
- 3+年三倍体ニジマス雌の成長優良性小林 徹 平成元年度滋賀県醒井養鱒場業務報告 42 -44 1990
- ニジマスの雌性発生における倍数化処理時期の検討-Ⅱ小林 徹 平成元年度滋賀県醒井養鱒場業務報告 36 -41 1990
- ニジマスの全雌三倍体量産と実用化に向けての特性解明,FCサイエンス,21世紀の養殖を科学する小林 徹 養殖 27- (12) 134 -137 1990
- 養殖ニジマスの在来系とドナルドソン系の遺伝的特徴,4. 魚介類の集団遺伝学的解析藤尾芳久; 中島正道; 藤岡康弘; 小林 徹 アイソザイムによる魚介類の集団解析,海洋生物集団の識別等に関する先導的評価手法の開発事業報告書 322 -333 1989
- マス類の人為倍数体利用による育種に関する研究-Ⅲ小林 徹; 津村祐二; 木村忠亮; 伊東正夫; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 1989
- '89年産大型卵系,早期・晩期産卵系雌性発生二倍体の成熟に伴う産卵形質の調査及び第二代雌性発生二倍体の作出小林 徹 昭和63年度滋賀県醒井養鱒場業務報告 58 -67 1989
- 第一卵割阻止によるアマゴの雌性発生二倍体について小林 徹 昭和63年度滋賀県醒井養鱒場業務報告 48 -57 1989
- 三倍体アマゴの成長・成熟並びにパー・スモルト相分化について小林 徹 昭和63年度滋賀県醒井養鱒場業務報告 34 -40 1989
- 性転換雄の精巣内精子に対する運動活性の向上について小林 徹 昭和63年度滋賀県醒井養鱒場業務報告 29 -33 1989
- マス類の人為倍数体利用による育種に関する研究-Ⅱ小林 徹; 八木久則; 木村忠亮; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 34pp 1988
- 大型卵系,早期・晩期産卵系の飼育経過と個体識別による優良成長個体の選抜小林 徹 昭和62年度滋賀県醒井養鱒場業務報告 62 -69 1988
- 三倍体ニジマスの血液性状等の季節的変動について小林 徹 昭和62年度滋賀県醒井養鱒場業務報告 48 -61 1988
- ニジマスの雌性発生における倍数化処理時期の検討小林 徹 昭和62年度滋賀県醒井養鱒場業務報告 39 -47 1988
- マス類の人為倍数体利用による育種に関する研究-Ⅰ小林 徹; 八木久則; 伏木省三 水産庁地域バイオテクノロジー研究開発促進事業報告書 38pp 1987
- ニジマス・アマゴ・イワナ間での異質3倍体作出による新品種の開拓小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 25 -26 1987
- 雌性発生による大型卵系,早期・晩期産卵系の作出小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 25 1987
- 3倍体魚類の呼吸生理-Ⅰ,3倍体アマゴの酸素消費量と再蓋運動回数の平常時・過激運動後での変動小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 24 1987
- 3倍体ニジマスの成熟度変化小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 23 -24 1987
- 3倍体ニジマスの成長と餌料効率小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 22 -23 1987
- ニジマス精子におけるDNA不活性化大量処理の試み小林 徹 昭和61年度滋賀県醒井養鱒場業務報告 22 1987
Books and other publications
- The Encyclopedia of IchthyologyKOBAYASHI Toru (ContributorChapter 9 Genetics, Chromosome manipulation)Maruzen Publishing 2018/10
- 飼育水温がホンモロコ性分化関連遺伝子に及ぼす影響.芦田裕史; 木下政人; 小林 徹 (Joint work)グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 2013/03
- クロマグロ若齢期における成長とIGF-1遺伝子発現.佐伯 康; 岡田貴彦; 本領智記; 阿川泰夫; 澤田好史; 柳下直己; 小林 徹 (Joint work)グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 2013/03
- Molecular identification and tissue distribution of peroxisome proliferators activated receptor gamma transcript in cultured Thunnus orientalis.Agawa Y; Honryo T; Ishii A; Kobayashi T; Oku H; Sawada Y (Joint work)グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 2013/03
- マダイにおける生殖巣形成過程.グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点 2008-2009(平成20-21)年度中間成果報告書,250KOBAYASHI Toru (250)Kinki Univ. Press 2010
- マダイPagrus majorの初期発生過程における形態形成と始原生殖細胞の動態.グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点 2008-2009(平成20-21)年度中間成果報告書,248-249KOBAYASHI Toru (248-249)Kinki Univ. Press 2010
- ホンモロコGnathopogon caerulescensを用いた核移植クローン作出技術の開発試行.グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点 2008-2009(平成20-21)年度中間成果報告書,246-247KOBAYASHI Toru (246-247)Kinki Univ. Press 2010
- 養殖クロマグロThunnus orientalisの遺伝的多様性と家系判別.グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点 2008(平成20-21)年度中間成果報告書,246-247Kobayashi T; Agawa Y; Sawada Y (246-247)Kinki Univ. Press 2010
- Developmental biology and genetics in aquaculture. The 21st Century COE Program Kinki University "Center of Aquaculture Science and Technology for Bluefin Tuna and Other Cultivated Fish" Final report:2003-2007. ;31-37Kobayashi T; Ueno K; Yagishita N; Otani S (31-37)The 21st Century COE Program Kinki University, Wakayama,Japan. 2008
- Construction of a BAC library for the Pacific bluefin Tuna Thunnus orientalis. Ecology and Aquaculture of Bluefin Tuna (Proceedings of the Joint international symposium on Bluefin Tuna, 2006. Edited by W.Sakamoto, S. Miyashita, and Y. Nakagawa). pp85-88Yagishita N; Oohara I; Kobayashi T (85-88)Kinki Univ. Press 2007
- Gonadal formation and development in cultured chub mackerel, Scomber Japonicus. Ecology and Aquaculture of Bluefin Tuna (Proseedings of the Joint international symposium on Bluefin Tuna, 2006. Edited by W.Sakamoto, S. miyashita, and Y. Nakagawa). pp79-84Kobayashi T; Ishibashi R; Yamamoto S; Ueno K; Murata O (79-84)Kinki Univ. Press 2007
- クロマグロの卵割期発生課程.21世紀プログラム クロマグロ等の魚類養殖産業支援型研究拠点 2003-2004(平成15-16)年度中間成果報告書,35-38Kobayashi T; Otani S; Miyashita S (35-38)Kinki Univ. Press 2005
- ホンモロコを用いた始原生殖細胞の移植技法開発.21世紀プログラム クロマグロ等の魚類養殖産業支援型研究拠点 2003-2004(平成15-16)年度中間成果報告書,31-34Otani S; Ohara M; Kobayashi T (31-34)Kinki Univ. Press 2005
- 借腹養殖技法開発を目的としたマダイの胚発生に関する基礎的研究.21世紀プログラム クロマグロ等の魚類養殖産業支援型研究拠点 2003-2004(平成15-16)年度中間成果報告書,23-26Kobayashi T; Hasegawa T; Kato K; Otani S (23-26)Kinki Univ. Press 2005
- 借腹レシピエントとしてのマサバの生殖巣分化について.21世紀COEプログラム クロマグロ等の魚類養殖産業支援型研究拠点.2003-2004(平成15-16)年度中間成果報告書,19-22Kobayashi T; Ishibashi R; Otani S; Murata O (19-22)Kinki Univ.Press 2005
Lectures, oral presentations, etc.
- 琵琶湖に放流された養殖ウナギ 3(耳石バックカリキュレーションによる成長解析)加澤渚; 高作圭汰; 松田直往; 石崎大介; 田辺祥子; 小林徹; 光永靖; 亀甲武志日本水産学会大会講演要旨集(CD-ROM) 2024
- 琵琶湖に放流された養殖ウナギ 4(銀化の可能性)小田康平; 高作圭汰; 加澤渚; 岸脇由宇太; 田辺祥子; 石崎大介; 光永靖; 小林徹; 亀甲武志日本水産学会大会講演要旨集(CD-ROM) 2024
- 琵琶湖に放流された養殖ウナギ 2(食性)高作圭汰; 花木基子; 加澤渚; 田辺祥子; 石崎大介; 光永靖; 小林徹; 亀甲武志日本水産学会大会講演要旨集(CD-ROM) 2024
- 琵琶湖に放流された養殖ウナギ 1(大型個体出現のメカニズム)高作圭汰; 花木基子; 加澤渚; 田辺祥子; 石崎大介; 光永靖; 小林徹; 亀甲武志日本水産学会大会講演要旨集(CD-ROM) 2024
- Female mimicry of biwayosinobori Rhinogobius biwaensis , Lake Biwa. [Not invited]松井謙弥; 寺田麗香; 前圭士郎; 山崎琉ノ介; 石崎大介; 甲斐嘉晃; 小林徹; 亀甲武志2023年度日本魚類学会年会 2023/09
- Feeding ecology of Ictalurus punctatus in southern Lake Biwa and the Seta River [Not invited]Keita TAKASAKU; Motoko HANAKI; Nagisa KAZAWA; Kenta UEDA; Daisuke ISHIZAKI; Yasushi MITSUNAGA; Toru KOBAYASHI; Takeshi KIKKO2023年度日本魚類学会年会 2023/09
- ゲノム編集技術を用いた旨味成分増量魚の作出 [Not invited]村上 悠; 安藤正史; 二股良太; 堀部智久; 植田和光; 木下政人; 小林 徹令和5年度日本水産学会春季大会 2023/03
- 琵琶湖に放流された養殖ウナギの成長と漁獲開始年齢高作圭汰; 花木基子; 加澤渚; 石崎大介; 光永靖; 小林徹; 田辺祥子; 亀甲武志日本水産学会大会講演要旨集(CD-ROM) 2023
- 琵琶湖で放流された養殖ウナギの成長と漁獲加入年齢. [Not invited]高作圭汰; 花木基子; 加澤渚; 石崎大介; 小林徹; 光永靖; 亀甲武志令和4年度日本水産学会近畿支部後期例会 2022/12
- The diet of channel catfish Ictalurus punctatus in southern Lake Biwa and the Seta River 2 (stable isotope analysis) [Not invited]Keita TAKASAKU; Motoko HANAKI; Nagisa KAZAWA; Kenta UEDA; Daisuke ISHIZAKI; Yasushi MITSUNAGA; Toru KOBAYASHI; Takeshi KIKKOthe 56th Annual Meeting, 2022; the Ichthyological Society of Japan. 2022/09
- The diet of channel catfish Ictalurus punctatus in southern Lake Biwa and the Seta River 1 (analysis of stomach contents) [Not invited]Keita TAKASAKU; Motoko HANAKI; Nagisa KAZAWA; Kenta UEDA; Daisuke ISHIZAKI; Yasushi MITSUNAGA; Toru KOBAYASHI; Takeshi KIKKOthe 56th Annual Meeting, 2022; the Ichthyological Society of Japan. 2022/09
- 低分子化合物を利用したダブルノックインメダカの作出 [Not invited]村上 悠; 小林 徹令和4年度日本水産学会春季大会 2022/03
- 雌性発生ホンモロコ卵への多回高温刺激処理による卵割阻止 [Not invited]小林 徹; 亀甲武志令和4年度日本水産学会春季大会 2022/03
- The diet of channel catfish Ictalurus punctatus in southern Lake Biwa and the Seta River 2 (stable isotope analysis)高作圭汰; 花木基子; 加澤渚; 上田健太; 石崎大介; 光永靖; 小林徹; 亀甲武志日本魚類学会年会講演要旨 2022
- The diet of channel catfish Ictalurus punctatus in southern Lake Biwa and the Seta River 1 (analysis of stomach contents)高作圭汰; 花木基子; 加澤渚; 上田健太; 石崎大介; 光永靖; 小林徹; 亀甲武志日本魚類学会年会講演要旨 2022
- 高温二回処理による雌性発生ホンモロコ倍加半数体の作出効果. [Not invited]小林 徹; 竹田秋都; 原貫太令和3年度日本水産学会春季大会 2021/03
- ホンモロコにおける雌性発生と通常発生の発生進行比較 [Not invited]小林 徹; 渡邊祐大; 窪田龍之介平成31年度日本水産学会春季大会 2019/03
- ホンモロコ卵卵割阻止のための2回高温処理適正強度および処理後の発生進行. [Not invited]小林 徹; 奥野 有希平成30年度日本水産学会春季大会 2018/03
- ホンモロコ卵の第一卵割に対する2回の高温処理の阻止効果. [Not invited]藤井ひかる; 山崎景也; 小林 徹平成28年度日本水産学会秋季大会 2016/09
- マアナゴ Conger myriasterにおける成長の雌雄差. [Not invited]小林 徹; 猪熊 徹; 瀬川 叡; 山田伸一; 家戸敬太郎; 太田博巳平成28年度日本水産学会春季大会 2016/03
- タナゴ亜科魚類の性分化に関する研究-Ⅰ(ニッポンバラタナゴ Rhodeus ocellatus kurumeus の 生殖巣形成および性分化に関する組織学的観察). [Not invited]松田征也; 高田瑞穂; 山口陽子; 小林 徹日本魚類学会年会 2015/09
- 高温処理によるホンモロコ受精卵の卵割阻止. [Not invited]小林 徹; 猪熊 徹; 大橋昭汰; 藤井ひかる平成17年度日本水産学会春季大会 2015/03
- 養成キハダ胚期の形態形成と生殖細胞の動態. [Not invited]小林 徹; 本領智記; 阿川泰夫; 澤田好史; Tapia I; Adames K; Cano A; Scholey V; Marguries D; 柳下直己日本水産学会春季大会 2014/03
- Effect of Water Temprature on Willow Minnow Sex Differentiation-related Genes in the Early Developmental Stages. [Not invited]Ashida, H; Kinoshita, M; Kobayashi, T第36回日本分子生物学会年会 2013/12
- Genetic population structure of the yellowfin tuna Thunnus albacares in the North Pacific Ocean. [Not invited]Nomura S; Kobayashi T; Agawa Y; Margulies D; Scholey V; Sawada Y; Yagishita Y9th Indo-Pacific Fish Conference 2013/06
- 抗酸化物質注射が魚肉のメト化と物性に及ぼす影響. [Not invited]内本良平; 塚正泰之; 懸橋理枝; 伊藤智広; 石橋泰典; 小林 徹; 安藤正史日本水産学会春季大会 2013/03
- クロマグロ初期発育期における骨形成関連遺伝子,BMP-2およびオステオカルシンの発現動態. [Not invited]齋藤佑介; 本領智記; 阿川泰夫; 澤田好史; 小林 徹日本水産学会春季大会 2013/03
- 飼育水温がホンモロコ性分化遺伝子の発現に与える影響. [Not invited]芦田裕史; 木下政人; 小林 徹日本水産学会春季大会 2013/03
- 養成キハダの生殖巣形成過程. [Not invited]小林 徹; 本領智記; 阿川泰夫; 澤田好史; Tapia I; Adames K; Cano A; Scholey V; Marguries D; 柳下直己日本水産学会春季大会 2013/03
- Identification of a Male Characteristics DNA Marker of Aquacultured Pacific Bluefin Tuna, Thunnus orientalis. [Not invited]Yasuo Agawa; Takafumi Komiya; Tomoki Honryo; Toru Kobayashi; Michio Kurata; Tokihiko Okada; Yoshifumi SawadaWorld Aquaculture 2013 2013/02
- クロマグロの骨格形成過程. [Not invited]齋藤佑介; 阿川泰夫; 澤田好史; 小林 徹日本水産学会秋季大会 2012/09
- クロマグロおよびキハダの北太平洋における遺伝的集団構造. [Not invited]野村尚平; 小林 徹; 阿川泰夫; Dan Margulies; Vernon Scholey; 澤田好史; 柳下直己日本水産学会秋季大会 2012/09
- ISSR分析によるクロマグロ養殖集団の遺伝的多様性. [Not invited]安井愛子; 柳下直己; 阿川泰夫; 澤田好史; 小林 徹日本水産学会秋季大会 2012/09
- 高水温環境下におけるホンモロコの性分化遺伝子の発現動態. [Not invited]芦田裕史; 木下政人; 小林 徹日本水産学会秋季大会 2012/09
- Expression profile of four willow minnow sex-related genes in the early developmental including sex differentiation stages. [Not invited]Ashida H; Kinoshita N; Kobayashi TAquaculture Europe 2012 2012/09
- クロマグロの硬骨形成過程. [Not invited]齋藤佑介; 阿川泰夫; 澤田好史; 小林 徹日本水産学会春季大会 2012/03
- ホンモロコ仔稚魚における性分化関連遺伝子の発現動態. [Not invited]芦田裕史; 木下政人; 小林 徹日本水産学会春季大会 2012/03
- キハダの卵内発生速度と孵化所要時間に及ぼす水温の影響. [Not invited]本領智記; Guillen A; Ibarra J; Margulies D; Scholey V; 小林 徹; 澤田好史日本水産学会春季大会 2012/03
- Out Line of Research Plan in Spawning Ecology Team [Not invited]Kobayashi TProgram for SATREPS Kickoff Symposium “Comparative Studies of the Reproductive Biology and Early Life History of Two Tuna Species for the Sustainable Use of these Resources” 2011/11
- The transcript quantity of PPAR gamma gene is correlated with lipid content in the original muscles of Pacific bluefin tuna Thunnus orientalis which indicates its role of master regulator in adipose cell proliferation. [Not invited]Agawa Y; Honryo T; Ishii A; Kobayashi T; Kurata M; Okada T; Miyashita S; Murata O; Kumai H; Sawada YAquaculture Europe 2011 2011/10
- Construction of a BAC library and development of microsatellite markers for the Pacific bluefin tuna Thunnus orientalis. [Not invited]Yagishita N; Sawada Y; Soeda E; Kobayashi TAquaculture Europe 2011 2011/10
- Foxl 2 and Dmrt 1 genes isolated from willow minnow Gnathopogon caerulescens: cloning, expression in various tissues, and role in developmental stages. [Not invited]Ashida H; Ueyama N; Kinoshita N; Kobayashi TAquaculture Europe 2011 2011/10
- Gonadal formation and premeiotic proliferation of gonocytes during sex-differentiation of willow minnow Gnathopogon caerulescens. [Not invited]Kobayashi TAquaculture Europe 2011 2011/10
- 活魚の血管に抗酸化物質を注入することによる冷蔵中の魚肉の品質保持. [Not invited]加藤圭祐; Bimol Chandra Roy; 石橋泰典; 小林 徹; 伊藤智広; 安藤正史; 塚正泰之日本水産学会秋季大会 2011/10
- スジシマドジョウ大型種の骨格形成過程. [Not invited]齋藤佑介; 隅田健夫; 松田征也; 小林 徹日本水産学会秋季大会 2011/09
- クロマグロ若齢魚における成長および水温の変化とIGF-1遺伝子発現. [Not invited]佐伯 康; 岡田貴彦; 本領智記; 阿川泰夫; 澤田好史; 柳下直己; 小林 徹日本水産学会秋季大会 2011/09
- ホンモロコ性分化関連遺伝子の発現解析. [Not invited]芦田裕史; 上山直城; 木下政人; 小林 徹日本水産学会秋季大会 2011/09
- クロマグロIGF遺伝子のクローニングと発現解析. [Not invited]佐伯 康; 岡田貴彦; 本領智記; 阿川泰夫; 澤田好史; 柳下直己; 小林 徹日本水産学会春季大会 2011/03
- ホンモロコの性分化に関与する遺伝子, Foxl 2, Dmrt 1の単離と発現解析. [Not invited]芦田裕史; 上山直城; 木下政人; 小林 徹日本水産学会春季大会 2011/03
- Molecular cloning and expression analysis of lipid cell differentiation factor, peroxisome proliferator activated receptor gamma in cultured pacific Bluefin tuna Thunnus orientalis. [Not invited]Yasuo Agawa; Tomoki Honryo; AyakoIshii; Toru Kobayashi; Michio Kurata; Tokihiko Okada; Shigeru Miyashita; Osamu Murata; Hidemi Kumai; Yoshifumi SawadaWorld Aquaculture Society 2011/02
- ハリヨの水族館飼育継代群の遺伝的多様性について. [Not invited]松田征也; 小林 徹水族館技術者研究会 2011
- クロマグロperoxisome proliferator-activated receptor gamma のクローニングと分子系統学的解析. [Not invited]阿川泰夫; 本領智記; 小林 徹; 倉田道雄; 岡田貴彦; 澤田好史日本水産学会 2010/03
- ホンモロコvasa, 42Sp50, CYP19a遺伝子クローニングと発現解析 [Not invited]上山 直城; 小林 徹; 芦田 裕史; 木下 政人; 村井 直正日本水産学会 2009/09 平成21年度日本水産学会大会(北里大,岩手) 日本水産学会
- Morphogenesis and behavior of primordial germ cells in the early embryonic development of the Japanese red sea bream Pagrus major. [Not invited]Kobayashi T; Yoshino IAquaculture Europe 2009 2009/08 Aquaculture Europe 2009, Trondheim, Norway Aquaculture Europe
- Transgenic Willow Minnow Strains Expressing Carp Growth Hormone Gene Driven by Medaka Beta-Actin Promoter for Rapid Production of Several Gene Expression Analysis Systems [Not invited]小林 徹; 石井 雅幸; 西村 直次; 上野 紘一International Symposium, Genetics in Aquaculture 2009 10th International Symposium, Genetics in Aquaculture (ISGA 2009, in Bangkok, Thailand) International Symposium, Genetics in Aquaculture
- Formation of Chimera by Injection into the Pharyngula Embryo with the Hypoblast Cells from Other Blastula of Willow Minnow Gnathopogon caerulescens [Not invited]小林 徹日本水産学会 2008/10 5th World Fisheries Congress (WFC 2008,Yokohama) 日本水産学会
- イシダイにおける発生段階区分および体節形成期以降の始原生殖細胞の動態 [Not invited]小林 徹; 田中 裕己; 吉野 一平; 村田 修日本魚類学会 2008/09 2008年度日本魚類学会年会(愛媛大,松山) 日本魚類学会
- マダイにおける原腸胚期以降の始原生殖細胞の動態 [Not invited]吉野 一平; 小林 徹平成20年度日本水産学会大会 2008/03 平成20年度日本水産学会大会(東海大,清水) 日本水産学会
- Cryopreservation of blastomeres for surrogate reproduction of endangered freshwater fish [Not invited]小林 徹; 山田 豊; 上野 紘一Aquaculture Europe 2007/10 Aquaculture Europe 2007 (Istanbul, Turkey) Aquaculture Europe
- クロマグロにおける始原生殖細胞の移動と生殖巣形成 [Not invited]小林 徹; 向井 良夫; 八木 洋樹; 山本 眞司; 宮下 盛; 上野 紘一; 村田 修日本水産学会 2007/04 平成19年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- ホンモロコの生殖巣形成過程 [Not invited]小林 徹; 上野 紘一日本水産学会 2007/04 平成19年度日本水産学会春季大会(東京海洋大,港区) 日本水産学会
- マサバの初期発生過程および始原生殖細胞の動向 [Not invited]小林 徹; 湯川 直子; 山本 眞司; 村田 修; 上野 紘一日本水産学会 2007 平成19年度日本水産学会秋季大会(北海道大,函館) 日本水産学会
- Gonadal Formation, Differentiation, and Development of Cultured Chub Mackerel [Not invited]小林 徹; 石橋 亮; 山本 眞司; 上野 紘一; 村田 修Kinki University 21st Century COE Program 2006 International Symposium 2006/11 Amami Setouchi City Joint Symposium on Bluefin Tuna, 2006. JA Hall, Setouchi, Kagoshima, Japan Kinki University 21st Century COE Program 2006 International Symposium
- 海産魚類の初期胚操作のための技法開発 [Not invited]大谷 哲; 家戸 敬太郎; 宮下 盛; 小林 徹平成18年度日本水産学会大会 2006/04 平成18年度日本水産学会大会(高知大,高知市) 日本水産学会
- 養殖マサバの生殖巣発達周期 [Not invited]小林 徹; 石橋 亮; 山本 眞司; 大谷 哲; 上野 紘一; 村田 修日本水産学会 2006/04 平成18年度日本水産学会大会(高知大,高知市) 日本水産学会
- ホンモロコの胚体形成期胚への嚢胚胚盤下部細胞の注入によるキメラ形成 [Not invited]小林 徹; 水野 敦; 大谷 哲; 上野 紘一日本水産学会 2006/04 平成18年度日本水産学会大会(高知大,高知市) 日本水産学会
- 水環境中における内分泌撹乱作用の検出と因子解析(1)-下水道処理水が流入する休耕田におけるエストロゲン活性と魚類への影響評価 [Not invited]肥田嘉文; 小林 徹; 蔵田高大; 山田祐輝; 金 桂花; 國松孝男; 白石不二雄; 白石寛明日本水環境学会 2006/03 第40回日本水環境学会年会(東北学院大学土樋キャンパス) 日本水環境学会
- 卵発生と胚操作技術.21世紀COEプログラム 平成17年度第5回シンポジウム「クロマグロの持続的養殖生産,肉質・安全性そして流通」 [Not invited]小林 徹21世紀COEプログラム成果報告シンポジウム 2006/02 平成17年度COE成果報告シンポジウム(近畿大学ノーヴェンバーホール,東大阪市) 21世紀COEプログラム成果報告シンポジウム
- Gonadal Formation and Development of Cultured Chub Mackerel for the Surrogate Reproduction of Bluefin Tuna. [Not invited]小林 徹; 石橋 亮; 山本 眞司; 上野 紘一; 村田 修Aquaculture CanadaOM 2006 Aquaculture CanadaOM 2006 - November 19 - 22, (Halifax, Nova Scotia, CANADA) Aquaculture CanadaOM
- RAPD法によるキンギョの系統解析 [Not invited]高田 肇; 鈴木 誉士; 小林 徹; 上野 紘一日本動物遺伝育種学会 2006 日本動物遺伝育種学会第7回大会(鹿児島大,鹿児島市) 日本動物遺伝育種学会
- 内湖とその周辺水路に出現するギンブナ仔稚魚のクローン系統の組成とその季節変化 [Not invited]鈴木 誉士; 小林 徹; 上野 紘一日本魚類学会 2006 2006年度日本魚類学会年会(東海大,静岡市) 日本魚類学会
- ISSR分析によるムサシトミヨ水族館飼育群の遺伝的多様性 [Not invited]小林 徹; 小笠原 央; 布施 幸江; 松田 征也日本魚類学会 2006 2006年度日本魚類学会年会(東海大, 静岡市) 日本魚類学会
- ホンモロコにおける胚盤積層移植法および胚体形成期細胞注入法によるキメラ形成 [Not invited]小林 徹; 大谷 哲; 水野 敦; 上野 紘一日本動物遺伝育種学会 2005/09 日本動物遺伝育種学会第6回大会(北海道大,札幌市) 日本動物遺伝育種学会
- 内湖とその周辺水路に出現するコイ・フナ仔稚魚の種組成 [Not invited]鈴木 誉士; 小林 徹; 上野 紘一日本魚類学会 2005/09 2005年度日本魚類学会年会(東北大学,仙台市) 日本魚類学会
- トランスジェニックの水産学的応用-GH発現系を例として- [Not invited]小林 徹日本水産学会近畿支部前期例会シンポジウム「魚類研究を中心としたトランスジェニック技術-基礎から応用へ-」 2005/07 日本水産学会近畿支部前期例会シンポジウム「魚類研究を中心としたトランスジェニック技術-基礎から応用へ-」(大阪市立大文化交流センター,大阪市)
- ドジョウの同一胞胚由来の細胞核移植によるクローン作成 [Not invited]田中 大介; 高橋 明人; 小林 徹; 上野 紘一日本水産学会 2005/04 平成17年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- マダイにおける初期発生過程 [Not invited]長谷川 徹; 大谷 哲; 大原 学; 上野 紘一; 家戸 敬太郎; 小林 徹日本水産学会 2005/04 平成17年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- ホンモロコの初期発生段階 [Not invited]大原 学; 大谷 哲; 長谷川 徹; 上野 紘一; 小林 徹平成17年度日本水産学会大会 2005/04 平成17年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- ホンモロコ始原生殖細胞の起源と動態 [Not invited]大谷 哲; 中島 聖; 上野 紘一; 小林 徹日本水産学会 2005/04 平成17年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- クロマグロにおける卵割から嚢胚形成にいたる胚発生過程 [Not invited]小林 徹; 大谷 哲; 大原 学; 長谷川 徹; 上野 紘一; 向井 良夫; 八木 洋樹; 宮下 盛日本水産学会 2005/04 平成17年度日本水産学会大会(東京海洋大,港区) 日本水産学会
- フローサイトメーターを用いた外国産シジミ類とマシジミとの区別方法の検討 [Not invited]松田 征也; 小林 徹; 大原 健一国際湿地再生シンポジウム 2005 滋賀県立琵琶湖博物館 国際湿地再生シンポジウム
- 人工採卵マサバの性分化過程 [Not invited]小林 徹; 大谷 哲; 山本 眞司; 米島 久司; 上野 紘一; 村田 修; 石橋 亮日本水産学会 2005 平成17年度日本水産学会大会(東京海洋大、港区) 日本水産学会
- クロマグロの胚発生と育種 [Not invited]小林 徹近畿大学 2005 近畿大学21世紀COEプログラム平成16年度第7回シンポジウム「マグロ養殖産業の未来に向けて,平成15・16年度成果報告シンポジウム(近畿大学ノーヴェンバーホール、東大阪市) 近畿大学
- 植物工場と養殖の複合生産システムの開発-閉鎖循環系での栽培・生育環境の検討 [Not invited]中村 謙治; 小林 徹農業環境工学関連4学会2004年合同大会 2004/09 農業環境工学関連4学会2004年合同大会(福岡国際会議場、福岡市) 農業環境工学会
- 琵琶湖につながる農業用水路に出現するコイ・フナ仔稚魚の種組成とその季節変化 [Not invited]鈴木 誉士; 小林 徹; 上野 紘一日本魚類学会 2004 2004年度日本魚類学会年会(琉球大、那覇市) 日本魚類学会
- 日本産コイ科モロコ類・ヒガイ類のmtDNA cytochrome B領域前半部の塩基配列分析とPCR-RFLPを用いた種判別 [Not invited]小林 徹; 上野 紘一; 永井 宏介; 松田 征也日本魚類学会 2004 2004年度日本魚類学会年会(琉球大,那覇市) 日本魚類学会
- Early embryonic development of willow minnow [Not invited]大谷 哲; 大原 学; 長谷川 徹; 中島 聖; 服部 千尋; 上野 紘一; 小林 徹日本動物学会 2004 日本動物学会75回大会(甲南大、神戸市) 日本動物学会
- Growth characteristics in F2 generation of transgenic willow minnow induced carp growth hormone gene [Not invited]西村 直次; 石井 雅幸; 大原 学; 上野 紘一; 小林 徹平成16年度日本水産学会大会 2004 平成16年度日本水産学会大会(鹿児島大) 日本水産学会
- Reproductive characteristics of mitotic gynogenetic diploid of rainbow trout [Not invited]亀甲武志; 中山耕至; 小林 徹; 井戸本純一平成16年度日本水産学会大会 2004 日本水産学会(鹿児島大) 平成16年度日本水産学会大会
- Reaction of metallothionein promoter as melanin-concentrating Hormone Expressing reporter in Transgenic fish to zinc ion [Not invited]五十嵐茂樹; 宇座英慈; 木下政人; 上野 紘一; 小林 徹第26 回日本分子生物学会年会(神戸) 2003/12 第26 回日本分子生物学会年会(神戸)
環境水中の金属イオン汚染物質の検出のためのバイオセンサーとして、肝臓での有毒金属イオン排出反応を体表色素胞のメラニン凝集反応として検出できるトランスジェニック系統の作出を検討し、亜鉛イオンに対して体色を明化応答する個体が得られた。 - Identification and population structure of Carassius larvae in Lake Biwa using RAPD analysis [Not invited]鈴木誉士; 永野元; 小林 徹; 上野 紘一平成15 年度日本魚類学会年会(京都) 2003/10 平成15 年度日本魚類学会年会(京都)
RAPD 分析によりフナ仔魚を種判別し、これらの頻度を琵琶湖沿岸のヨシ帶6 か所と農業用水路3 か所の計9 か所で比較して、各フナ仔魚の生息場所の差異および湖内における集団構造について解析した。 - Production of Transgenic Willow Minnow Strain Containing Corp Growth Hormone Gene Driven by Medaka Beta-Actin Promoter for Rapid Preparation of Several Gene Expression Analysis Systems [Not invited]小林 徹; 石井雅幸; 上野 紘一第6 回マリンバイオテクノロジー国際学会、第5 回アジア太平洋マリンバイオテクノロジー学会(千葉) 2003/09 第6 回マリンバイオテクノロジー国際学会、第5 回アジア太平洋マリンバイオテクノロジー学会(千葉)
遺伝子発現解析モデルとしてのトランスジェニック魚の系統化促進のため、メダカのβアクチンプロモーターに制御されたコイ成長ホルモン遺伝子を卵にマイクロインジェクションし、これらF0 世代およびF1 世代の成長について、それぞれ通常魚と比較した。 - Enhanced growth and shortening of period required for sexual maturation of transgenic willow minnow induced carp growth hormone gene [Not invited]西村直次; 大原学; 上野 紘一; 小林 徹第9 回小型魚類研究会(埼玉) 2003/09 第9 回小型魚類研究会(埼玉)
遺伝子発現解析モデルとしてのトランスジェニック魚の系統化促進のため、コイ成長ホルモン遺伝子導入ホンモロコの作出経過のうち、雌雄各2 個体のF1 からF2 世代をそれぞれ作出した。F2 世代は通常魚と遜色ない発生を示した。 - Morphological and genetic characteristics of nigorobuna crucian carp collected from Nishinoko, satellite lake of lake Biwa [Not invited]鈴木誉士; 永野元; 小林 徹; 上野 紘一平成15年度日本水産学会大会(東京) 2003/04 平成15年度日本水産学会大会(東京)
琵琶湖北湖とその内湖である西の湖に生息する異なるタイプのニゴロブナの形態学的、遺伝学的差異を検討した。後者は前者よりも鰓耙が少なく頭部が小さく、気道弁は未発達であったが、mtDNA、D-loop領域のRFLP分析では両者に差は見られなかった。 - Cytological observation of nuclear and chromosomal behavior in gynogenetic crucian carp eggs exposed to heat or cold shock for retention of second polar body [Not invited]小林 徹; 根本守仁; 上野 紘一Genetics in Aquaculture Ⅷ(International Association for Genetics in Aquaculture)(チリ) 2003 Genetics in Aquaculture Ⅷ(International Association for Genetics in Aquaculture)(チリ)
第二極体放出阻止開始の低温処理および高温処理の適正時期、核の行動について検討した。低温処理および高温処理の最適時期は、それぞれ嫌精後7 分および9 分であった。両区とも卵によって、染色体胞、半数性前核、二倍性前核などが様々観察された。 - F2 groups of transgenic willow minnow containing carp growth hormone gene with accelerated growth and shortened alternating period of generation [Not invited]西村直次; 大原学; 上野 紘一; 小林 徹第26 回日本分子生物学会年会(神戸) 2003 第26 回日本分子生物学会年会(神戸)
トランスジェニックにより作出を検討している成長促進ホンモロコ系統のF2 世代の形態的特徴および群内成長変異について報告した。特に作出した4 系統のうち雌のF1 から作出した1 系統が著しい成長を示した。 - Production of transgenic fish line for detecting of the heavy metal pollutants in aquatic environments [Not invited]五十嵐茂樹; 西村直次; 吉川亜矢; 義山百合香; 木下政人; 上野 紘一; 小林 徹第25回日本分子生物学会年会(横浜) 2002/12 第25回日本分子生物学会年会(横浜)
環境水中の重金属汚染物質を検出するトランスジェニック魚系統を作出するために、メタロチオネインプロモーター発現系に着目し、受精卵へのDNAマイクロインジェクションによりpb?actin cassette EGFP(β?actin)、pCMT?EGFP(CMT)、pCMV?MCH(CMV)およびprtMT?MCH(rtMT)をそれぞれ導入したホンモロコを作出して、これらを用いて環境モニタリングへの応用を検討した。 - Karyotypes and nuclear DNA contents of two lizardfish species [Not invited]岸岡稚青; 小林 徹; 上野 紘一染色体学会第53回大会(奈良) 2002/10 染色体学会第53回大会(奈良)
ヒメとミナミアカエソの通常核型、分染核型を分析し、両種にZW型性染色体をみいだした。核型とDNA量に基づきエソ亜目魚類の性染色体分化について論じた。 - Effects of enhanced growth of transgenic F0 Gnathopogon caerulescens containing an exogenous growth hormone gene of Cyprinus carpio [Not invited]石井雅幸; 野村弘昭; 別所伸昭; 上野 紘一; 小林 徹第 6 回日本マリンバイオテクノロジー学会大会 (小金井) 2002/05 第 6 回日本マリンバイオテクノロジー学会大会 (小金井)
コイの成長ホルモン遺伝子を導入したホンモロコの孵化後 16 週までの成長を通常のものと比較したところ、 4 交配実験区のうち 2 つで有意な差が観察され、 顕著な場合は平均体重で対照の 3.4 倍に成長したが、 実験群では下垂体の縮少が観察された。 - Injection of Carp growth hormone gene into honmoroko eggs for rapid induction of transgenic strain [Not invited]小林 徹; 上野 紘一; 別所伸昭; 野村弘昭; 石井雅幸第 6 回日本マリンバイオテクノロジー学会大会 (小金井) 2002/05 第 6 回日本マリンバイオテクノロジー学会大会 (小金井)
成長促進系統作出による対象種の世代交代期間短縮化を目的に、 コイ成長ホルモン遺伝子を含む plasmid をホンモロコ卵へ導入した。 導入遺伝子の保有率は 40%で、 孵化時の体サイズに差はなかった。 RT PCR により孵化後 30 日から導入遺伝子の転写が確認された。 - Improvement of sperm motility of sex reversed male rainbow trout by incubation in high pH artificial spermatoplasma [Not invited]小林 徹; 伏木省三; 上野 紘一Genetics of Subpolar Fish and Invertebrates, 20th Lowell Wakefield Fisheries Symposium (アメリカ) 2002/05 Genetics of Subpolar Fish and Invertebrates, 20th Lowell Wakefield Fisheries Symposium (アメリカ)
ニジマスの輸精管内精子と精巣内精子とを様々な pH の人工精漿に懸濁させた場合の BSS 中での運動時間の変化を調べた。 高 pH の人工精漿による精子の培養は、 精管の欠損した性転換雄精巣内精子の運動活性を向上させ、 全雌および全雌三倍体の作出効率を向上させた。 - Selection of F0 for production of transgenic F1 generation: correlation of success of gene induction to the fin and the germ cell [Not invited]Toru Kobayashi; Koichi Ueno; Ayako Nishizawa平成 14 年度日本水産学会大会 (奈良) 2002/04 平成 14 年度日本水産学会大会 (奈良)
トランスジェニック系統早期確立のための F0 親魚選抜指標として尾鰭を用いたスクリーニング法を検討した。 尾鰭に導入遺伝子を保有している個体は生殖巣にも保有している確率が高く、 F1 作出のための F0 選抜には鰭細胞を用いた PCR が有効と示唆された。 - Expression of GFP gene drived by several promoters induced to honmoroko eggs [Not invited]西澤彩子; 上野 紘一; 小林 徹平成 14 年度日本水産学会大会 (奈良) 2002/04 平成 14 年度日本水産学会大会 (奈良)
ホンモロコにウイルス由来および魚類由来の制御配列を連結した GFP 遺伝子を導入し、 異なる生物種、 異なるプロモータ間での発現の強さや特異性 (時期・部位) について比較した。 系統的に近い種の制御系を用いることにより導入遺伝子の効果的制御が可能になる。 - Heteromorphic sex cyoromosomes and nuclear DNA contents of lizardfish [Not invited]上野 紘一; 小林 徹; 小嶋吉雄染色体学会第 52 回大会 (米子) 2001/11 染色体学会第 52 回大会 (米子)
エソ科魚類の核型分化を DNA 量の関係、 異型性染色体の所有による DNA 量の性差等について論じた。 - RAPD分析による琵琶湖産フナ属の種および交雑魚の判別 [Not invited]鈴木誉士; 小林 徹; 遠藤 誠; 三枝 仁; 上野紘一平成12年度日本魚類学会年会 2000/10
- 1回または2回の高温処理による雌性発生ニジマス卵第一卵割阻止の細胞学 [Not invited]小林 徹; 桑田知宣平成12年度日本水産学会春季大会 2000/04
- 琵琶湖産フナ属3亜種のmtDNA,D-Loop領域の遺伝的変異 [Not invited]鈴木誉士; 小林 徹; 小林敬典; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- RAPD分析による琵琶湖産フナ属3亜種の遺伝的変異量と種判別 [Not invited]小林 徹; 鈴木誉士; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- 魚類の雄性発生個体mtDNAの遺伝的由来 [Not invited]大野豪久; 小林 徹; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- オキエソのZZ-ZW1W2型複合性染色体の起源と分化 [Not invited]上野紘一; 太田欽也; 小林 徹平成11年度日本水産学会秋季大会 1999/09
- ホンモロコに導入した緑色蛍光タンパク(GFP)遺伝子の次世代への伝達 [Not invited]小林 徹; 吉田尚史; 西澤彩子; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- ドジョウの生殖系列キメラ [Not invited]中川雅博; 小林 徹; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- 魚類初期胚細胞の凍結保存 [Not invited]太田裕介; 小林 徹; 上野紘一平成11年度日本水産学会秋季大会 1999/09
- 琵琶湖産フナ属3亜種における形態形質差の再検討およびmtDNA,D-Loop領域RFLPの差異 [Not invited]小林 徹; 鈴木誉士; 根本守仁; 上野紘一平成11年度日本水産学会近畿支部前期例会 1999/07
- キメラ魚の成長に伴う標識メラノフォアの消長 [Not invited]中川雅博; 小林 徹; 上野紘一平成11年度日本水産学会春季大会 1999/04
- クローン雌性発生アマゴ初期胚における第二極体放出阻止限界時期の細胞学的観察 [Not invited]小林 徹; 上野紘一平成10年度日本水産学会秋季大会 1998/09
- フエダイ属2種の染色体研究 特にロクセンフエダイにみられた複合性染色体について [Not invited]太田欽也; 上野紘一; 小林 徹平成10年度日本水産学会秋季大会 1998/09
- アカエソ属の2種にみられた雌異型染色体 [Not invited]太田欽也; 上野紘一; 小林 徹平成10年度日本水産学会春季大会 1998/04
- 初期胚細胞の移植におけるドジョウのキメラ作製 [Not invited]上野紘一; 北尾和久; 小林 徹平成10年度日本水産学会春季大会 1998/04
- ホンモロコ卵に導入したGFP遺伝子の発現 [Not invited]吉田尚史; 上野紘一; 小林 徹平成9年度日本水産学会秋季大会 1997/09
- ヒラメの第一卵割阻止型雌性発生誘起に関する細胞学的検証 [Not invited]上野紘一; 小林 徹; 山本栄一平成9年度日本水産学会秋季大会 1997/09
- シーケンサを用いた蛍光RAPD-PCR自動解析によるクローン魚系統の迅速判定 [Not invited]小林 徹; 小林敬典平成10年度日本水産学会春季大会 1997/04
- DNAシーケンサを用いた蛍光RAPD-PCR分析 [Not invited]小林敬典; 大野聖爾; 小林 徹第6回DNA多型学会学術集会 1997
- 人為的雌性発生操作および倍数化操作がニジマス卵発生核の挙動および卵割時期に及ぼす影響 [Not invited]小林 徹平成5年度日本水産学会秋季大会 1993/09
- 紫外線照射ニジマス精子を用いた雌性発生アマゴ卵の発生初期における細胞学的観察 [Not invited]小林 徹平成5年度日本水産学会秋季大会 1993/09
- 雌性発生育種によるアマゴのパー・スモルト形質の二世代目の育種効果 [Not invited]小林 徹; 伏木省三平成4年度日本水産学会春季大会 1992/04
- 雌性発生法によるクローンアマゴ量産の可能性. [Not invited]小林 徹; 伏木省三平成3年度日本水産学会春季大会 1991/04
- 雌性発生アマゴの成長変異とパー・スモルト変態. [Not invited]小林 徹; 伏木省三平成2年度日本水産学会秋季大会 1990/09
- サケ科魚類における生殖内分泌学的研究-Ⅴ, 性分化期の二倍体, 三倍体ニジマス卵巣における細胞学的観察. [Not invited]小林 徹; 伏木省三; 長濱嘉孝平成2年度日本水産学会春季大会 1990/04
- 雌性発生法を用いた育種に関する研究-Ⅰ, 親子回帰法による再生産関連形質の遺伝性. [Not invited]小林 徹; 伏木省三; 山田行雄平成元年度日本水産学会秋季大会 1989/09
- サケ科魚類における生殖内分泌学的研究-Ⅳ, エストロジェン投与による三倍体ニジマス雌のビテロジェニン産生 [Not invited]小林 徹; 原 彰彦; 伏木省三; 長濱嘉孝平成元年度日本水産学会秋季大会 1989/09
- サケ科魚類における生殖内分泌学的研究-Ⅲ, 3+年三倍体ニジマス卵巣における卵黄の蓄積. [Not invited]小林 徹; 伏木省三; 酒井則良; 長濱嘉孝平成元年度日本水産学会春季大会 1989/04
- サケ科魚類における生殖内分泌学的研究-Ⅱ, 三倍体ニジマスの精巣発達過程. [Not invited]小林 徹; 伏木省三; 酒井則良; 長濱嘉孝平成元年度日本水産学会春季大会 1989/04
- サケ科魚類における生殖内分泌学的研究-Ⅰ, 三倍体ニジマス精巣のステロイド産生能 [Not invited]小林 徹; 酒井則良; 長濱嘉孝; 伏木省三昭和63年度日本水産学会春季大会 1988/04
- マラカイトグリーンの発癌性検定-Ⅱ, ニジマス:陰性. [Not invited]小林 徹; 木村郁夫; 伏木省三; 安藤 学; 石田広次昭和63年度日本水産学会春季大会 1988/04
- マラカイトグリーンの発癌性検定-Ⅰ, マウス:陰性. [Not invited]木村郁夫; 安藤 学; 石田広次; 伏木省三; 小林 徹昭和63年度日本水産学会春季大会 1988/04
Affiliated academic society
Research Themes
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)Date (from‐to) : 2022/04 -2025/03Author : Toru Kobayashi
- 純系養殖品種作出のための簡易な卵割阻止法の開発 -微小管の再生メカニズムの解明ー日本学術振興会:科学研究費補助金Date (from‐to) : 2014/04 -2017/03Author : 小林 徹
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2008 -2011Author : KINOSHITA Masato; KOBAYASHI TouruWe have cloned vasa, 52Sp50, cyp19a, dmrt1, foxl2 gene from Hon-moroko(Gnathopogon caerulescens). The expression level of 42Sp50 gene, which are know as an oocytes specific gene, gradually increased as sex-differentiation process was advanced and significantly differed among individuals. From these facts, we recognized that the 42Sp50 gene was a good indicator for female and that the individuals expressing 42Sp50 gene must be female.
- Identification and Breeding of Race Specific Expressing Genes using Gynogenetic
- Study of Physiological Function of Fish using Transgenic Technology
- Production of Clonal Fish Using Androgenetic Procedure and the Inheritance
- Production of Chimera Fish by Transplant of Early Embryonic Cells.
- Microinjection of Exogenous Gene to Fish Eggs and Production of Transgenic Fish Strain