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KANZAKI Shinya
Department of Agricultural Science | Professor |
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- 果樹園芸学 Pomology
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- Kosuke Shimizu; Tetsuya Matsukawa; Risa Kanematsu; Kimihisa Itoh; Shinya Kanzaki; Shigeru Shigeoka; Shin'ichiro KajiyamaBioscience, biotechnology, and biochemistry 2021/05 [Refereed]
Headspace solid-phase microextraction combined with GC/MS (HS-SPME-GC/MS) is one of the strongest tools for comprehensive analysis of volatile compounds and has been used to analyze aromatic components of mango and investigate its varietal characteristics. In this study, profiling of aroma compounds in 17 mango cultivars, grown in the same green house to exclude the effect of environmental factors, was conducted and the patterns were subjected to principal component analysis (PCA) to identify the relationship between the aroma components and cultivars. Fifty-nine different volatile constituents were detected from the blends of these 17 mango cultivars. The cultivars were divided into four clusters using PCA based on the volatile components determined in the study. Aiko was found to mainly contain δ-3-carene and showed a composition more similar to its pollen parent, Irwin, than to its seed parent, Chiin Hwang No. 1. - 志水恒介; 巽賢太郎; 宇都宮直樹; 神崎真哉; 伊藤仁久; 重岡成熱帯農業研究 14 (1) 1882-8434 2021 [Refereed]
- Kosuke Shimizu; Kentaro Tatsumi; Naoki Utsunomiya; Shinya Kanzaki; Kimihisa Itoh; Shigeru ShigeokaHorticultural Research (Japan) Japanese Society for Horticultural Science 20 (1) 87 - 94 1347-2658 2021/01 [Refereed]
- Shinya Kanzaki; Asuka Ichihi; Yuta Tanaka; Shiina Fujishige; Sota Koeda; Kosuke ShimizuScientia Horticulturae Elsevier BV 272 109567 - 109567 0304-4238 2020/10 [Refereed]
The skin color of mature mango (Mangifera indica L.) fruit varies from green to red depending on cultivars. Red coloration of mango fruit skin is due to anthocyanin accumulation and is known to be induced by light exposure as in some other fruit crops. Recently, several investigations on the genes involved in anthocyanin biosynthesis in mango fruit skin have been conducted, to understand the molecular mechanism underlying red coloration of the mango fruit. In the present study, we investigated the characteristics of four R2R3-MYB transcription factors (TF) including MiMYB1, which is assumed to be the MYB TF regulating anthocyanin biosynthesis in mangoes. The deduced amino acid sequence of MiMYB1 showed a high degree of similarity with the anthocyanin-related R2R3-MYB TFs from other plant species and contained two conserved motifs defining the anthocyanin-related MYB TFs. Expression analysis in the mango fruit skin under different light conditions showed that the expression of MiMYB1 increased as the intensity of light exposure increased, parallel to anthocyanin accumulation and the expression of some structural genes of the anthocyanin biosynthetic pathway. In addition, a transient expression assay of tobacco leaves showed that co-infiltration of MiMYB1 and MibHLH2, which is a bHLH TF of mango, induced anthocyanin accumulation in tobacco leaves. These results suggest that MiMYB1 acts as the key regulator for anthocyanin biosynthesis in mango fruit skin, and that light-dependent red coloration of mango fruit skin is regulated by MiMYB1 in transcript levels. - Sota Koeda; Ikuya Fujiwara; Yuki Oka; Elly Kesumawati; Sabaruddin Zakaria; Shinya KanzakiPlant Disease Scientific Societies 104 (12) 3221 - 3229 0191-2917 2020/06 [Refereed]
Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus that originated in the eastern Mediterranean, has spread worldwide, becoming a serious threat to tomato (Solanum lycopersicum L.) production. Southeast Asia is considered one of the hotspots for begomovirus diversity, and a wide variety of local begomovirus species distinct from TYLCV have been identified. In this study, the protection effect of introgressions of single TYLCV Ty resistance genes, Ty-2 and Ty-3a, in tomato was examined against inoculations of the bipartite begomoviruses Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) and Pepper yellow leaf curl Indonesia virus (PepYLCIV) isolated from Indonesia. Our findings suggest that Ty-2 in the heterozygous state was found to be ineffective against PepYLCIV and TYLCKaV, whereas Ty-3a in the heterozygous state was effective against PepYLCIV and partially effective against TYLCKaV. Quantification of viral DNAs showed correlation between symptom expression and viral DNA accumulation. Moreover, mixed infections of TYLCKaV and PepYLCIV caused notably severe symptoms in tomato plants harboring Ty-3a. In cases of mixed infection, quantifying viral DNAs showed a relatively high accumulation of PepYLCIV, indicating that Ty-3a loses its effectiveness against PepYLCIV when TYLCKaV is also present. This study demonstrates the lack of effectiveness of Ty resistance genes against single and mixed infections of distinct local begomoviruses from Southeast Asia. - Ayako Katayama-Ikegami; Zion Byun; Suzuka Okada; Masahiro Miyashita; Takane Katayama; Tomoaki Sakamoto; Asuka Ichihi; Kosuke Shimizu; Shinya KanzakiThe Horticulture Journal Japanese Society for Horticultural Science 2189-0102 2020 [Refereed]
Red mangos (Mangifera indica L.) accumulate appreciable amounts of cyanidin-based anthocyanins in the skin, and previous studies showed that these anthocyanins contain galactose as a sugar moiety. To date, two UDP:flavonoid 3-O-glycosyltransferase (UFGT)-like genes named MiUFGT1 and MiUFGT3 (MiUFGalT3) have been isolated from mango 'Irwin' peel as anthocyanin-related UFGT genes, but the function of the proteins of the genes have not yet been elucidated. In this study, we characterized recombinant MiUFGT1 and MiUFGalT3 expressed in Escherichia coli. In the presence of quercetin as an acceptor, rMiUFGT1 showed marginal glucosylation activity, while rMiUFGalT3 exhibited significant galactosylation activity 20-fold higher than its glucosylation activity. Specificity analysis using purified MiUFGalT3 found that rMiUFGalT3 almost equally accepts anthocyanidins and flavonols. The anthocyanins extracted from the 'Irwin' skins were cyanidin 3-O-galactoside and 7-O-methylcyanidin 3-O-galactoside by instrumental analyses, which is consistent with previous results obtained for other red mango cultivars. The results suggest that MiUFGalT3 is responsible for the red coloration of 'Irwin' mango fruit skins.
- Sota Koeda; Ryutaro Nakano; Takaya Sawaki; Kosuke Sato; Yoshiyuki Tanaka; Shinya KanzakiThe Horticulture Journal Japanese Society for Horticultural Science 89 (4) 460 - 465 2189-0102 2020 [Refereed]
- Hoa Tran; Shinya Kanzaki; Ludwig Triest; Inaki Hormaza; Na Jong Kuk; Ray Ming; Jean Bousquet; Damase Khasa; Patrick Van DammeGenetic Resources and Crop Evolution 66 1653 - 1669 0925-9864 2019/12 [Refereed]
© 2019, Springer Nature B.V. We report on 14 microsatellites enriched in CT repeats obtained from a genomic library of lychee (Litchi chinensis Sonn.) cultivar “Hong Huay”. The polymorphisms revealed by these microsatellites were evaluated in a collection of 45 local Vietnamese lychee varieties and 4 Xerospermum noronhianum (Blume) Blume (Sapindaceae) collected from the wild. Samples were collected from local villages and forests in northern Vietnam. Genetic diversity parameters were estimated for the local Vietnamese varieties analyzed. The unweighted pair-group method of clustering using averages divided the lychee cultivars into three main groups: Cluster 1 (Group A) consisting of semi-natural lychees (“extremely early” lychee); Cluster 2 (Group B) consisting of cultivated cultivars (“intermediate” lychee); and Cluster 3 (Group C) representing X. noronhianum accessions. Using STRUCTURE, three subpopulations were also delimited among litchi accessions, including accessions with extremely early- and intermediate/late-maturing traits showing membership coefficients above 0.99 for Cluster 1 and Cluster 2, respectively. Accessions with early- and intermediate-maturing traits were identified as admixture forms with varying levels of membership shared between the two clusters, indicating their hybrid origin during litchi domestication. This is the first report on transferability of SSR markers developed from lychee (L. chinensis) to X. noronhianum. Results demonstrate the usefulness of microsatellites for identification, genetic diversity analysis and germplasm conservation in lychee and related Sapindaceae forest species. - Elly Kesumawati; Shoko Okabe; Kanami Homma; Ikuya Fujiwara; Sabaruddin Zakaria; Shinya Kanzaki; Sota KoedaArchives of virology 164 (9) 2379 - 2383 0304-8608 2019/09 [Refereed]
During 2017, leaf samples of chili pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants exhibiting yellowing and curling symptoms were collected from Aceh province, Indonesia. These samples were used to isolate and sequence viral genomic DNA. Six isolates with complete DNA-A and DNA-B sequences of begomovirus were obtained, all of which showed >99% sequence identity to the others. DNA-A sequences shared the highest nucleotide sequence identity (89.3%-89.7%) with monopartite pepper yellow leaf curl Indonesia virus 2 (PepYLCIV2) and the second-highest sequence identity (87.3%-87.4%) with bipartite pepper yellow leaf curl Indonesia virus (PepYLCIV). The DNA-B sequences shared the highest nucleotide sequence identity (95%-97.5%) with PepYLCIV. Results of recombination analysis indicated that the novel begomovirus was a recombinant. In accordance with the guidelines for begomovirus species demarcation, these isolates should be assigned to a new species, and we have proposed the name ''pepper yellow leaf curl Aceh virus'' (PepYLCAV) for this virus. - Shinya Kanzaki; Shiori Kamikawa; Asuka Ichihi; Yuta Tanaka; Kosuke Shimizu; Sota Koeda; Naoki UtsunomiyaThe Horticulture Journal Japanese Society for Horticultural Science 88 (4) 435 - 443 2189-0102 2019 [Refereed]
<p>The red coloration of the mango 'Irwin' skin is an important factor determining its value in the Japanese domestic luxury fruit market. In the present study, to investigate the molecular mechanism underlying anthocyanin biosynthesis of mango fruit skin, UFGT-like genes were isolated and the expression profile of anthocyanin-related genes was determined. Several UFGT-like genes were identified in transcriptome data of red 'Irwin' mango skin and two genes, <i>MiUFGT1</i> and <i>MiUFGT3</i>, were considered to be involved in mango skin coloration. Deduced amino acid sequences of these genes exhibited high similarity to other plant UFGTs and contained the conserved PSPG box common to the glycosyltransferase family. The presence of a glutamine and a histidine residue at the C-terminus end of the PSPG box in <i>MiUFGT1</i> and <i>MiUFGT3</i>, respectively, implied that <i>MiUFGT1</i> and <i>MiUFGT3</i> use glucose and galactose, respectively, as a sugar donor; however, the actual function and sugar donor preference of these enzymes remain to be elucidated. Expression analysis of anthocyanin-related genes during skin coloration suggested that <i>MiCHS</i> and <i>MiANS</i>, as well as <i>MiUFGT1</i> and <i>MiUFGT3</i>, play important roles in the anthocyanin biosynthesis of mango fruit skin and that the expression of these genes is regulated by the MYB transcription factor, as reported in other plant species.</p> - Sota Koeda; Kanami Homma; Yuri Tanaka; Daiki Onizaki; Elly Kesumawati; Sabaruddin Zakaria; Shinya KanzakiThe Horticulture Journal Japanese Society for Horticultural Science 87 (3) 364 - 371 2189-0102 2018 [Refereed]
© 2018 The Japanese Society for Horticultural Science (JSHS), All rights reserved. Indonesia is one of the world’s largest fresh pepper (Capsicum spp.) fruit-producing countries, and hot peppers are essential spices in Indonesian cuisine. During the last two decades, begomovirus, which is transmitted by the whitefly, Bemisia tabaci (Gennadius), and causes pepper yellow leaf curl disease, began to cause a huge economic loss by damaging pepper plants in Indonesia. In the present study, a highly efficient inoculation method was established for Pepper yellow leaf curl Indonesia virus (PepYLCIV), the most infectious bipartite begomovirus in pepper plants cultivated in North Sumatra, by combining agroinoculation and subsequent grafting. Partial tandem repeats of PepYLCIV DNA A and B were constructed and cloned into a binary pGreenII vector, and their infectivity was tested. Co-inoculation of Nicotiana benthamiana L. and Solanum lycopersicum L. ‘Momotaro’ with PepYLCIV DNA A and DNA B resulted in the production of typical begomoviral symptoms. Both the injection of the cotyledons with cultured agrobacteria and the inoculation of the hypocotyl with agrobacterial colonies induced viral symptoms in pepper No. 218 (C. annuum L.) seedlings in approximately 55–75%. When agroinoculated symptomatic No. 218 was grafted onto an uninfected ‘Takanotsume’ (C. annuum), all newly elongated shoots from the rootstock of ‘Takanotsume’ produced typical begomoviral symptoms. Agroinoculation combined with subsequent grafting provides a highly efficient method for introducing PepYLCIV into pepper plants. - Sota Koeda; Kanami Homma; Yuri Tanaka; Elly Kesumawati; Sabaruddin Zakaria; Shinya KanzakiHORTICULTURE JOURNAL JAPAN SOC HORTICULTURAL SCI 86 (4) 479 - 486 2189-0102 2017 [Refereed]
Tomato yellow leaf curl disease caused by begomoviruses is a serious threat to tomato (Solanum lycopersicum L.) production. If begomoviruses, transmitted by whitefly (Bemisia tabaci), infect tomato plants during early growth, production can be almost entirely lost. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus, is emerging as an important threat to solanaceous crop production in Southeast Asia. The lack of mechanical transmission of some begomoviruses is a major experimental constraint. In this study, an agroinoculation method using TYLCKaV in tomato plants was established. Partial tandem repeats of TYLCKaV DNA A and DNA B were constructed and cloned to a binary pGreenII vector, and their infectivity was tested. Co-inoculation of TYLCKaV DNA A and DNA B to Nicotiana benthamiana L. produced typical begomoviral symptoms, and both of the viral DNA components accumulated in the upper uninoculated leaves, suggesting systemic infection of TYLCKaV. Two agroinoculation methods were conducted on tomatoes. First, excised sections of tomato shoots were agroinoculated with a soaking procedure. Although two Agrobacterium tumefaciens strains were tested, approximately 40% of inoculated plants only showed viral symptoms for EHA105. Second, agrobacterium from a cultured petri dish was directly inoculated with a colony inoculation procedure. When EHA105 was used, approximately 92% of inoculated plants showed viral symptoms. Sequencing the recovered viral DNA from the upper uninoculated leaf clarified that TYLCKaV had successfully infected the tomato plants. The colony inoculation procedure is labor-saving, and viral symptoms develop in susceptible tomato plants within approximately a month from sowing the seeds. This method could contribute to simple and speedy evaluation of TYLCKaV resistance of tomato plants. - Dan E. Parfitt; Keizo Yonemori; Chitose Honsho; Mitsunori Nozaka; Shinya Kanzaki; Akihiko Sato; Masahiko YamadaTREE GENETICS & GENOMES SPRINGER HEIDELBERG 11 (2) 1614-2942 2015/04 [Refereed]
A molecular marker analysis for Asian persimmon cultivar relationships and pollination status was conducted with 496 amplified fragment length polymorphism (AFLP) markers and 146 cultivars of Asian origin. Cultivars from China, Korea, and Japan were evaluated for marker composition and pollination status, which strongly influences fruit characteristics. Separation of Chinese, Korean, and Japanese cultivar groups and pollination type by neighbor-joining clustering, multidimensional scaling, and STRUCTURE was only weakly supported and not genetically significant. Significant differences for cultivar origin and pollination status were found for analysis of molecular variance (AMOVA), but most of the variation was among cultivars, not classification groups. All of the cultivar groups were genetically similar at the molecular level with most polymorphism due to individual cultivar differences. - Nobuhito Mitani; Atsushi Kono; Masahiko Yamada; Akihiko Sato; Shozo Kobayashi; Yusuke Ban; Toshihito Ueno; Mikio Shiraishi; Shinya Kanzaki; Tomoyuki Tsujimoto; Keizo YonemoriHORTSCIENCE AMER SOC HORTICULTURAL SCIENCE 49 (9) 1132 - 1135 0018-5345 2014/09 [Refereed]
Persimmon (Diospyros kaki Thunb) is hexaploid, and the pollination-constant, non-astringent (PCNA)/non-PCNA trait of Japanese origin is qualitatively controlled by the AST/ast alleles at a single locus and the PCNA trait is recessive to the non-PCNA trait. To avoid inbreeding depression led by repeated crosses among PCNA genotypes, non-PCNA genotypes should be used as cross parents. The marker-assisted selection system has been developed for the selection of PCNA offspring in the progeny derived from the cross of non-PCNA 'Taigetsu' (non-PCNA 'Kurokuma' X PCNA 'Taishu') to PCNA 'Kanshu'. The primer pairs E8.5/E9r and 7H9F/AST-R were used for detecting the molecular markers A(1) and A(3), respectively, which link AST alleles. Complete agreement was found between the sequence-characterized amplified region (SCAR) marker genotype and fruit astringency phenotype of the 48 offspring. The result confirmed that the marker-assisted selection using those markers was highly practical. In a larger offspring population (522 offspring) from the same cross, offspring segregated into 100 with both markers, 162 with only A(1), 179 with A(3), and 81 with neither, and this segregation ratio was significantly different from 2:3:3:2, which is the segregation ratio of random chromosome assortment in autohexaploid. The percentage of offspring expected to be PCNA was 15.5% (81 of 522), which was slightly lower than 20%. - Nobuhito Mitani; Atsushi Kono; Masahiko Yamada; Akihiko Sato; Shozo Kobayashi; Yusuke Ban; Toshihito Ueno; Mikio Shiraishi; Shinya Kanzaki; Tomoyuki Tsujimoto; Keizo YonemoriSCIENTIA HORTICULTURAE ELSEVIER SCIENCE BV 170 219 - 223 0304-4238 2014/05 [Refereed]
Pollination-constant, non-astringent (PCNA) type persimmons (Diospyros kaki Thunb.) steadily lose fruit astringency and are more desirable than other types of persimmons. The PCNA/non-PCNA trait is qualitatively controlled by AST/ast alleles at a single locus. Persimmons are hexaploid, and PCNA of Japanese origin and non-PCNA genotypes are denoted as aaaaaa and A----, respectively, if AST gene denotes A and ast gene denotes a. 'Taiten' is an F-1, non-PCNA persimmon offspring with genotype AAaaaa derived from PCNA x non-PCNA, and molecular markers linked AST alleles in 'Taiten' shows polymorphism as A(2) and A(3), so that the genotype of 'Taiten' can show A(2)A(3)aaaa using the alleles of molecular marker. Offspring from a backcross of 'Taiten' x 'Kanshu' (PCNA) were evaluated. The astringency type was analyzed phenotypically, by fruit sensory and brown specks tests, and genotypically by SCAR markers with PCR primer pairs (E4/A2r for detecting the SCAR marker A(2) and 7H9F/AST-R for both A(2) and A(3)). The ratio of PCNA offspring was 43/251 (17.1%), and there was discrepancy between the phenotypic evaluation and SCAR marker estimation in only 3 offspring. The ratio was not significantly different from the theoretical ratio of nulliplex in autohexaploid. The PCNA offspring segregation ratio was 36/151 (23.8%) for 'Fuyu' (PCNA) x 'Taiten' and 6/29 (20.7%) for 'Tenjingosho' (PCNA) x 'Taiten' (nearly the same ratio reported for 'Taiten' x 'Kanshu'). Marker-assisted selection using SCAR markers is thus effective and practical in persimmon breeding. (C) 2014 Elsevier B.V. All rights reserved. - Kanzaki Shinya; Inoue Kouichi; Utsunomiya Naoki; Yano MasayoshiHort. Res. (Japan) THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 12 (1) 1 - 7 1347-2658 2013 [Refereed]
In this study, a set of seven simple sequence repeat (SSR) markers were developed for Japanese maple, and the genetic diversity of 107 Japanese maple cultivars was estimated using these markers. A total of 87 alleles were detected, ranging from 4 to 25 per locus, with an average of 12.43 alleles per locus. Most cultivars showed unique SSR profiles, indicating that the markers were effective for cultivar identification, while some pairs of bud-sport cultivars and synonyms showed identical SSR profiles. Principal component analysis (PCA) indicated that 107 Japanese maple cultivars were divided into two groups: one consists of cultivars of Acer palmatum, and the other group includes cultivars of two varieties, A. amoenum var. amoenum and A. amoenum var. matsumurae. Analysis of molecular variance (AMOVA) revealed significant differentiation between the two species, while the genetic differentiation and distance between the two varieties of A. amoenum were very low, with FST = 0.026 and D = 0.057, respectively. Thus, the two varieties of A. amoenum are genetically indistinguishable from each other in domesticated cultivars, while most cultivars of A. palmatum have been developed separately from those of A. amoenum. - Masahiro Nakagawa; Chitose Honsho; Shinya Kanzaki; Kousuke Shimizu; Naoki UtsunomiyaSCIENTIA HORTICULTURAE ELSEVIER SCIENCE BV 139 108 - 117 0304-4238 2012/05 [Refereed]
Mango (Mangifera indica L.) is one of the most important evergreen fruit trees, but it has a high tendency of biennial bearing because of irregular flowering. In this study, a FLOWERING LOCUS T-like (FT-like) gene was isolated from mango (MiFT) and characterized. The deduced amino acid sequence of MiFT showed high identity of the gene to other plant FT-like genes, and further, MiFT expression increased only in the leaves under floral-inductive conditions. Comparison of heavy crop load (HC) and no crop load (NC) mango trees showed that MiFT expression strongly increased only in NC trees under cool temperature. In NC trees, almost all the apical buds formed panicles, whereas in HC trees, only a few panicles were produced in the next season of fruit set. Further. HC trees had lower starch content in the shoots than NC trees. Furthermore, application of 250-ppm gibberellin 3 (GA(3)) completely inhibited flowering and MiFT expression in both HC and NC trees. GA metabolism genes were also isolated from mango and their expression patterns were investigated. Gibberellin-3-oxidase (GA3-ox) controls the final step of biosynthesis of active GA, and its gene expression surged only in HC trees under cool temperature. In conclusion, MiFT is considered a key factor in mango flowering, and regulation of MiFT expression through GA metabolism was discussed. (C) 2012 Elsevier B.V. All rights reserved. - Chitose Honsho; Marie Inada; Ken-ichi Yuji; Masahiro Tojiki; Shigefumi Kurogi; Shinya Kanzaki; Takuya TetsumuraJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 81 (1) 27 - 34 1882-3351 2012/01 [Refereed]
Although Japanese mango is produced under a fairly unique cultivation system and fetches high prices at market, further growth of the mango industry in Japan requires the development of new cultivars. However, since individual mango flowers are quite small, no more than 1 cm in diameter, a large number of skilled workers is necessary for artificial pollination, thus making it difficult to obtain a sufficient number of progenies for breeding. We therefore evaluated a methodology whereby progenies were obtained by open pollination and their male parent was subsequently determined by DNA markers. Two mango cultivars ('Irwin' and 'Beni-Keitt') were arranged in a plastic house and honeybees were released as a pollen vector for open pollination. Harvested fruits were characterized and their seeds were sown in a bed. The male parent of the germinated seedlings was then identified by five simple sequence repeat (SSR) markers. As a result of SSR genotyping, the male parents of 185 of 239 'Irwin' seedlings were revealed; 79 were obtained by self-pollination and 106 from out-crossing. For 'Beni-Keitt', the male parent of 14 of the 20 seedlings was determined with two self-pollinated and 12 out-crossed. Preferential out-crossing in 'Irwin' was revealed using the chi-square test, although the considerable number of self-pollinated fruit obtained shows that a sufficient number of fruit can be set in a single-planted orchard. The effect of the male parent on fruit characteristics was subsequently examined, revealing that in 'Irwin' the Brix value of self-pollinated fruits was significantly higher than that of cross-pollinated fruits. Certain color values were, however, lower in self-pollinated 'Irwin' fruits. - Ayako Ikegami; Sai Eguchi; Takashi Akagi; Akihiko Sato; Masahiko Yamada; Shinya Kanzaki; Akira Kitajima; Keizo YonemoriJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 80 (2) 150 - 155 1882-3351 2011/04 [Refereed]
Persimmon cultivars are classified into four types depending on the nature of astringency loss of the fruit, namely pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA), pollination variant astringent (PVA), and pollination constant astringent (PCA). Among these four types, PCNA is the most important cultivar for persimmon breeding due to the stable loss of natural astringency on the tree. The trait of natural astringency loss is recessive in Japanese PCNA cultivars, while that in the Chinese PCNA cultivar, 'Luo tian tian shi', is dominant and the latter locus, termed CPCNA, is different from that in Japanese cultivars. In order to develop a molecular marker for the selection of the CPCNA-type cultivar, we performed amplified fragment length polymorphism (AFLP) in combination with bulked segregant analysis for F-1 offspring derived from 'Luo tian tian shi', which was used as the maternal parent. A total of 384 primer combinations were tested, and three AFLP markers, namely EACT-MCCC-222 (RO1), EGGC-MCTC-309 (RO2), and EGCC-MCGA-105 (RO3), linked to the CPCNA dominant allele were obtained. Among these markers, EGGC/MCTC-309 (RO2) was converted into a sequence-characterized amplified region (SCAR) marker. PCR analysis using F-1 offspring (n = 264) revealed that the relevance ratio of the SCAR marker was 94%. The polymorphism of the RO2 marker, which was strongly linked to the CPCNA dominant allele, was detected in only two Chinese PCNA cultivars, namely 'Luo tian tian shi' and 'Tian bao gai', among the Chinese, Korean, and Japanese cultivars tested. These results indicate that the RO2 marker contributes to marker-assisted selection for breeding programs of new PCNA cultivars having the CPCNA trait. - Shinya Kanzaki; Takashi Akagi; Takuya Masuko; Miyuki Kimura; Masahiko Yamada; Akihiko Sato; Nobuhito Mitani; Naoki Ustunomiya; Keizo YonemoriJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 79 (2) 150 - 155 1882-3351 2010/04 [Refereed]
In previous studies, we have developed molecular markers linked to the AST locus that controls fruit astringency type in persimmon; however, these markers are not feasible for practical application to breeding programs since they are not fully effective for discriminating the pollination constant and non-astringent (PCNA) genotype from the non-PCNA genotype in a progeny derived from 'Kurokuma'. Here we developed new sequence characterized amplified region (SCAR) markers that enable easy and reliable selection of the PCNA genotype from breeding populations. Genomic regions adjacent to probe 5R, which showed polymorphic fragments between PCNA and non-PCNA genotypes in restriction fragment length polymorphism (RFLP) analysis, were isolated from the genomic libraries of 'Nishimura-wase', 'Jiro', and 'Kurokuma'-derived offspring. The isolated genomic regions were characterized and 3 insertion/deletion mutations were observed between ast- and AST-linked regions. Several primers were designed in the flanking region of Indel-3 and, in multiplex PCR, it was shown that using 2 forward primers, AST-F and PCNA-F and a reverse primer, 5R3R, is the most useful and reliable primer set. The AST-linked 220-bp fragment proved to be a common marker of 'Kurokuma'-, 'Nisimura-wase'- and 'Aizumishirazu'- derived progenies. This multiplex PCR is considered the most practical tool for marker-assisted selection (MAS) and can enhance and accelerate progress in persimmon breeding. - Takashi Akagi; Yumi Takeda; Keizo Yonemori; Ayako Ikegami; Atsushi Kono; Masahiko Yamada; Shinya KanzakiJOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE AMER SOC HORTICULTURAL SCIENCE 135 (1) 59 - 66 0003-1062 2010/01 [Refereed]
Persimmon (Diospyros kaki Thunb.) is generally hexaploid, and a single AST locus controls the pollination-constant non-astringency trait on each of six corresponding chromosomes. The pollination-constant non-astringent (PCNA) genotype is nulliplex and requires homozygous recessive alleles (ast) at the AST locus. There are several non-PCNA cultivars/selections that could be cross parents; however, the probability of yielding nulliplex offspring depends on the number of recessive alleles (ast). In genotyping for the AST locus in hexaploid persimmon, in contrast to the situation in diploid plants, we need to detect the AST/ast allele dosage; this cannot be detected by common codominant markers. In this study, we detected the allele dosage of M(ast), which is a marker allele strongly linked to the ast allele among cultivars, by quantitative real-time polymerase chain reaction (qPCR) using three reference sites, actin (DkAct), anthocyanin reductase (DkANR), and L5R, whose sequences are conserved in the genome of persimmon cultivars. Based on the allele dosage of the M(ast), AST/ast genotypes were estimated for 63 non-astringent cultivars/selections, of which only five cultivars/selections were estimated to be simplex or duplex. The quantitative genotyping method using qPCR may be generally effective for polyploid plants. - Takashi Akagi; Shinya Kanzaki; Mai Gao; Ryutaro Tao; Dan E. Parfitt; Keizo YonemoriTREE GENETICS & GENOMES SPRINGER HEIDELBERG 5 (3) 483 - 492 1614-2942 2009/07 [Refereed]
Persimmon (Diospyros kaki Thunb.) is a polyploidy fruit tree species of economic importance to East Asia. Natural astringency loss is an important trait in persimmon breeding programs. Quantitative real-time PCR was used to determine the number of AST/ast alleles for fruit astringency in persimmon (D. kaki Thunb.). To this end, the cultivar Jiro was transformed with one or two copies of a gene encoding NADP-dependent sorbitol-6-phosphate dehydrogenase (S6PDH), which was used as a standard for measuring the allele number of a sequenced marker tightly linked to the recessive ast locus for nonastringency. Primers for markers linked to the AST or ast allele were then used to measure the AST to ast ratio directly in the progeny of a full-sib cross. From determination of the AST to ast ratio and the results of the S6PDH copy number, the number of AST and ast alleles at the AST/ast locus was estimated. This research supported the hypothesis that D. kaki is a hexaploid with six AST and/or ast alleles. In addition to the determination of the allelic status of the AST locus, the application of real-time PCR for confirmation of the ploidy level and allelic composition of target genes in autopolyploids or allopolyploids was demonstrated. - Shinya Kanzaki; Masahiko Yamada; Akihiko Sato; Nobuhito Mitani; Naoki Ustunomiya; Keizo YonemoriJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 78 (1) 68 - 73 1882-3351 2009/01 [Refereed]
Persimmon (Diospyros kaki Thunb.) cultivars are classified into 4 types depending on the relationship between astringency of the mature fruit and the effect of seeds on the loss of astringency, and only pollination-constant and non-astringent (PCNA)-type persimmons stably lose fruit astringency as a part of fruit development. This is a recessive trait, regarded to be controlled by a single locus, namely, the AST locus, which has a polysomic nature. Thus far, we have identified 2 restriction fragment length polymorphism (RFLP) markers, namely, A1 and A2, each of which is separately linked to a different AST allele, and proved that the RFLP markers were useful for selecting PCNA-type persimmons. This study was conducted to convert the RFLP markers into polymerase chain reaction (PCR)-based markers. We isolated and characterized genomic regions corresponding to each RFLP marker by inverse PCR. Two primer pairs, E4/E9r and E4/A2r, were designed to generate 2 sequence characterized amplified region (SCAR) markers, i.e., PCR-A1 and PCR-A2, respectively. The PCR-A1 and PCR-A2 markers cosegregated with the A] and A2 markers, respectively, in 'Nishimura-wase'-derived progenies. Although the primer pair E4/A2r did not produce the PCR-A2 marker in the FU-275, which is a progeny derived from 'Aizumishirazu', all non-PCNA-type offspring but no PCNA-type offspring showed the PCR-A1 marker using the primer pair E4/E9r. Thus, it was revealed that the SCAR markers were useful for selecting PCNA-type offspring in these progenies. On the other hand, disruption of the relationship between the markers and the AST locus was observed in the KU-325, derived from 'Kurokuma', indicating that the selection of PCNA-type offspring using PCR-based markers is not effective for progeny derived from 'Kurokuma'. Herein, we discuss the possibility of applying PCR-based markers to genetic studies. - Wakisaka Masaru; Sugimura Teruhiko; Ishimori Tomoya; Kanzaki ShinyaHort. Res. (Japan) THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 8 (4) 427 - 432 1347-2658 2009 [Refereed]
We present a new nursery grafting method for persimmon (Diospyros kaki Thunb.) propagation. This study examined the effects of grafting time and leaves of rootstock on the graft-take ratio and growth of grafted plants. Between April and September, we were able to graft a scion of 'Fuyu' persimmon onto a current seedling of 'Hourenbou' rootstock, which was between 110 and 195 days old. Although grafting onto a current seedling without leaves yielded a low graft-take ratio, 80 to 100% of scions grafted onto rootstock with leaves were established successfully. Between rootstock with and without leaves, a significant difference was noted in the callus-forming ratios 20 days after grafting. A callus was observed in all nursery plants grafted onto rootstock with leaves, irrespective of the bud-break feasibility. - Keizo Yonemori; Chitose Honsho; Shinya Kanzaki; Hitofumi Ino; Ayako Ikegami; Akira Kitajima; Akira Sugiura; Dan E. ParfittTREE GENETICS & GENOMES SPRINGER HEIDELBERG 4 (2) 149 - 158 1614-2942 2008/04 [Refereed]
To elucidate the relationships among Diospyros kaki and species closely related in previous studies, the nuclear ribosomal internal transcribed spacer (ITS) DNA sequence and the chloroplast matK gene were sequenced and compared with those of nine Diospyros species from Thailand, four species from temperate regions, and one species of southern Africa, D. lycioides. Maximum parsimony, maximum likelihood, and neighbor joining analyses of the matK and ITS data sets revealed that D. kaki is closely related to two diploid species, D. oleifera and D. glandulosa. D. kaki, D. glandulosa, and D. oleifera were placed differently in the trees obtained from ITS and matK data sets, suggesting that hybridization and/or introgression may have occurred during the development of these species. D. kaki was not found to be closely related to D. ehretioides, a diploid species from Thailand. These results differed from a prior analysis of this genus performed with chloroplast DNA ( cpDNA) restriction site mutations in 3.2- and 2.1-kb amplified sequences. The results supported Ng's hypothesis that D. glandulosa and D. kaki may share a common ancestor. D. oleifera was also closely associated with D. kaki. - Shinya Kanzaki; Akihiko Sato; Masahiko Yamada; Naoki Utsunomiya; Akira Kitajima; Ayako Ikegami; Keizo YonemoriJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 77 (1) 28 - 32 1882-3351 2008/01 [Refereed]
We investigated marker-assisted selection of the pollination constant and non-astringent (PCNA) trait and inheritance modes of the marker locus in persimmon (Diospyros kaki Thunb.) using restriction fragment length polymorphism (RFLP) analysis. In two backcross progenies, FU-170 and FU-275, 5 of 71 and 23 of 101 offspring, respectively, were judged as PCNA genotype by the marker phenotype. The astringency type of individual offspring was determined by measuring the size of tannin cells and soluble tannin content in mature fruit, thus confirming complete co-segregation of RFLP markers and the non-PCNA phenotype in 32 and 68 fruit-obtainable offspring from FU-170 and FU-275, respectively; therefore, RFLP markers can be used for PCNA genotype detection. Their segregation ratio deviated significantly from that of disomic inheritance. We also discuss the possibility of polysomic inheritance of RFLP markers. - A study of the phylogenetic relationships of longan (Dimocarpus longan), using amplified fragment length polymorphisms (AFLP) analysis.Janthasri, R; S. Kanzaki; N.Utsunomiya; Subhadrabandhu, SThai J. Agric. Sci. 36 319 - 328 2003/07 [Refereed]
- Genetic variations among mangosteen (Garcinia mangostana L.) revealed by AFLP analysis.Yapwattanaphun, C; Kanzaki, S; Yonemori, K; Subhadrabandhu, SThai J. Agric. Sci. 36 329 - 338 2003/07 [Refereed]
- K Yonemori; C Honsho; S Kanzaki; W Eiadthong; A SugiuraPLANT SYSTEMATICS AND EVOLUTION SPRINGER-VERLAG WIEN 231 (1-4) 59 - 75 0378-2697 2002 [Refereed]
The phylogenetic relationships among 14 Mangifera L. species of Thailand were analyzed by comparing sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA). Parsimony and neighbor joining (NJ) analyses revealed that the common mango (M. indica L.) was closely related to M. laurina Bl., M. sylvatica Roxb., and Al. oblong folia Hook. f. Mangifera foetida Lour. and M. odorata Griff. were also related to M. indica in both parsimonious and NJ trees, although these two species are classified into a different subgenus (subgenus Limits) from the subgenus Mangifera to which M. indica belongs. ITS sequence analysis revealed that several species have nucleotide additivity (two different nucleotides at the same locus) at several sites in the ITS region. Also, M. indica had several polymorphisms among cultivars. This finding may suggest a possibility of hybrid origin of Mangifera species, although Mangifera species are all assumed to be diploid having chromosome number of 2n = 2x = 40. - S. Kanzaki; K. Yonemori; A. Sato; M. Yamada; A. SugiuraJournal of the Japanese Society for Horticultural Science THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 69 (6) 702 - 704 1882-3351 2000/12 [Refereed]
The cultivation of the PCNA (pollination-constant and non-astringent)-type persimmon is advantageous because of its natural loss of astringency on the tree. We previously found RFLP markers linked to the dominant alleles controlling the PCNA/non-PCNA trait and showed that the PCNA genotype could be distinguished from non-PCNA genotype by these markers in the breeding populations. In this study, RFLP markers were investigated with 33 persimmon cultivars. All PCNA cultivars except for Chinese 'Luo Tian Tian Shi', had identical banding pattern, whereas the non-PCNA cultivars exhibited many polymorphic bands. Thus, we could distinguish PCNA cultivars of Japanese origin from non-PCNA cultivars by the RFLP analysis, although 'Luo Tian Tian Shi' was mistakenly classified as a non-PCNA type. These results suggest that this marker system could be applied to distinguish PCNA cultivars of Japanese origin from non-PCNA ones. - S Kanzaki; K Yonemori; A Sato; M Yamada; A SugiuraJOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE JAPAN SOC HORTICULTURAL SCI 69 (6) 665 - 670 1882-3351 2000/11 [Refereed]
The genetic relationships among 19 pollination- constant and non- astringent (PCNA) cultivars and 14 non-PCNA cultivars of persimmon (Diospyros kaki Thunb.), including one Chinese PCNA cultivar 'Luo Tian Tian Shi', were analyzed by comparing 138 AFLP markers. A phylogenetic tree constructed based on the similarity indices of these AFLP markers indicated a close relationship among Japanese PCNA cultivars, but a more distant relationship with the Chinese PCNA cultivar 'Luo Tian Tian Shi'. The close relationship between PCNA cultivars native to Gifu prefecture was distinct, indicating that these cultivars developed from crosses among restricted sources in this region. The cultivars 'Gosho', 'Hana-gosho', 'Oo-gosho', and 'Yamato- gosho' showed a close relationship with some non-PCNA cultivars. - W Eiadthong; K Yonemori; S Kanzaki; A Sugiura; N Utsunomiya; S SubhadrabandhuJOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE AMER SOC HORTICULTURAL SCIENCE 125 (2) 160 - 164 0003-1062 2000/03 [Refereed]
The phylogenetic relationships among 14 Mangifera L. species including three economically important species, i.e., common mango (M, indica L,), horse mango (M. foetida Lour.) and kwini (M. odorata Griff,), were analyzed by comparing 217 amplified fragment length polymorphism (AFLP) markers. The unweighted pair grouping method using arithmetic averages (UPGMA) and neighbor-joining (NJ) method were used and two outgroup tare, cashew nut (Anacardium occidentale L,) and gandaria (Bouea macrophylla Griff.), were added to both analyses. The common mango was closely related to banana mango (M. sylvatica Roxb.), M. laurina Bl., and M. oblongifolia Hook.f. Intraspecific variation among seven cultivars of common mango was much smaller than interspecific variation and these cultivars were classified into one M, indica group using both methods. Mangifera macrocarpa Bl., M. foetida, and M. odorata were also related to M. indica in both UPGMA and NJ trees, although these three species are classified into a different subgenus (subgenus Limus) from the subgenus Mangifera to which M. indica belongs. Also, in both UPGMA and NJ trees, M. gedebe Mig. and M. griffithii Hk.f. were placed in distant positions among the Mangifera species tested, indicating these two species are related distantly to M. indica. The AFLP technique was confirmed to be useful for phylogenetic analysis. - S Kanzaki; K Yonemori; A Sugiura; S SubhadrabandhuJOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY HEADLEY BROTHERS LTD 73 (3) 317 - 321 0022-1589 1998/05 [Refereed]
Phylogenetic relationships among seven species of the genus Durio, of which four produce edible fruits including the common durian (D. zibethinus), were investigated from RFLP analysis of PCR products from a variable region of cpDNA. The target region of cpDNA was reliably amplified from all Durio species tested, using total DNAs extracted by a modified CTAB method of Doyle and Doyle (1987) followed by an additional purification step. When restriction fragment polymorphisms of amplified products were examined with 19 restriction endonucleases (AseI, BamHI, BfaI, DraI, EcoRI, EcoRV, HindIII, KpnI, MspI, RsaI, ScaI, ScrFI, SalI, SmaI, SspI, StyI, TaqI, XbaI, XhoI), RFLPs were observed with 14 endonucleases. However, since all Durio species tested had length mutation on this amplified region of cpDNA, the most parts of diversities from 14 endonucleases were caused by the length mutations among species. Considering length mutation among species, a total of seven site mutations were detected as well as 12 length mutations, based on D. zibethinus. When unordered parsimony analysis was conducted using these data, the cultivated durian (D. zibethinus) did not show any close relationships to other three species producing edible fruits. D. zibethimus formed a cluster with wild species, D. griffthii, and other three species made two clusters with D. lowianus or D. mansoni. Our results indicated an independent evolutionary process among four edible species of the genus Durio. - Keizo Yonemori; Shinya Kanzaki; Dan E Parfitt; Naoki Utsunomiya; Suranant Subhadrabandhu; Akira SugiuraGenome Canadian Science Publishing 41 (2) 173 - 182 0831-2796 1998/04 [Refereed]
The relationships among 17 Diospyros species from Thailand, with particular emphasis on the relationship of these species to temperate Diospyros species, including Diospyros kaki, were studied, using 81 cpDNA restriction site mutations detected in the 3.2- and 2.1-kb regions of amplified cpDNA and six different length mutations detected in the 2.1-kb region of amplified cpDNA. Parsimony and neighbor-joining analyses were conducted to identify relationships among species. Three temperate zone species, D. kaki, Diospyros lotus, and Diospyros virginiana, were monophyletic with one subtropical species, Diospyros ehretioides, suggesting a close evolutionary relationship among them. An immediate common progenitor for D. kaki and D. virginiana is suggested from cpDNA homology and the polyploidized karyotypes of both species. Our results did not support Ng's hypothesis that Diospyros glandulosa (synonym Diospyros roxburghii) is the progenitor of D. kaki. Two species, Diospyros rhodocalyx and Diospyros confertiflora, were so distant from the remaining species that additional study is needed to determine whether they should be placed in the same genus.Key words: cpDNA, Diospyros, PCR, phylogeny, persimmon, RFLP, taxonomy. - Naoki Utsunomiya; Suranant Subhadrabandhu; Keizo Yonemori; Masayoshi Oshida; Shinya Kanzaki; Fumiaki Nakatsubo; Akira SugiuraEconomic Botany New York Botanical Garden Press 52 (4) 343 - 351 0013-0001 1998 [Refereed]
Diospyros species distributed widely in Thailand were classified into four ecotypes, according to their habitat constantly humid area, alternately dry and wet area, mountainous cool area and all area. Some of them inhabit near dwelling areas or in the paddy field in the village. The young fruit is covered with dense pubescence in most species. The size, shape, and color of mature fruit greatly vary greatly with the species. In most species, the mature fruit has a soft pulp and hard skin. The fruit of six species has been used for dying. Four species produce edible fruits, with color and flavor favorable for breeding of edible Diospyros species. The fruit of some species contains some chemicals useful as fish poisoning or of medicines, although the active components have not yet been identified. The edible fruit contained many tannin cells, but the fruit used as fish poisoning and medicines had only a few. - S Kanzaki; K Yonemori; A Sugiura; S SubhadrabandhuSCIENTIA HORTICULTURAE ELSEVIER SCIENCE BV 70 (1) 57 - 66 0304-4238 1997/06 [Refereed]
The phylogenetic relationships among eleven Artocarpus species, including two economically important species, the jackfruit (A. heterophyllus Lamk) and the breadfruit (A. altilis Fosberg), were estimated by comparing 30 restriction-site mutations in an amplified region of chloroplast DNA (cpDNA). Neighbor-joining and parsimony analyses were performed on the data to identify the relationships. The jackfruit and chempedak (A. integer Merr.) were monomorphic and therefore indistinguishable for all restriction sites. This confirmed the close relationship between these two species. The breadfruit and A. elasticus formed a monophyletic group with strong support from both neighbor-joining and parsimony analyses, indicating the possibility that the breadfruit was derived from A, elasticus or its close relatives. In general, our results were in agreement with the conventional classifications in Artocarpus species except for the position of A. chaplnsha. (C) 1997 Elsevier Science B.V. - YONEMORI Keizo; PARFITT Dan e; KANZAKI Shinya; SUGIURA Akira; UTSUNOMIYA Naoki; SUBHADRABANDHU SuranantJournal of the Japanese Society for Horticultural Science THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 64 (4) 771 - 777 0013-7626 1996/03 [Refereed]
RFLP analysis of PCR products from a variable region of the chloroplast genome was conducted to determine their potential for use in phylogenic studies of the genus <I>Diospyros</I>. The target region of cpDNA was reliably amplified by the procedure of Arnold et al. (1991) from all 14 Diospyros species tested. When restriction fragment polymorphisms of amplified products were examined at the intraspecific level with 10 endonucleases (<I>Ase</I> I, <I>Bfa</I> I, <I>Bst</I> NI, <I>Dde</I> I, <I>Msp</I> I, <I>Nco</I> I, <I>Rsa</I> I, <I>Scr</I> FI, <I>Sty</I> I, and <I>Taq</I> I), 15 cultivars of persimmon (<I>D. kaki</I>) and 3 horticultural varieties of <I>D. lotus</I> were monomorphic within species. However, restriction fragment polymorphisms were observed at the interspecific level. The 14 <I>Diospyros</I> species tested could be divided into 6 groups by the digestion patterns with ScrF I, 5 groups with <I>Bst</I> NI or <I>Taq</I> I, 4 groups with <I>Bfa</I> I or <I>Sty</I> I, 3 groups with <I>Ase</I> I, <I>Dde</I> I, or <I>Nco</I> I, 2 groups with <I>Msp</I> I or <I>Rsa</I> I. These results clearly indicated that the present analysis method is extremely valuable for phylogenetic and evolutionary studies at the interspecific level of the genus <I>Diospyros</I>.
MISC
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- Chiaki Hara; Kenichi Tomi; Kosuke Shimizu; Shinya Kanzaki; Yutaka Tsuchihashi; Takahiro Hayashi CHEMICAL SENSES 41- (9) E263 -E264 2016/11 [Refereed]
- Shinya Kanzaki JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI 63- (7) 328 -330 2016 [Invited]
- 鎌谷昌明; 富研一; 神崎真哉; 志水恒介; 文室政彦; 林孝洋; 宇都宮直樹 園芸学研究 別冊 13- (1) 2014
- T. Tsujimoto; T. Akagi; K. Yonemori; S. Kanzaki V INTERNATIONAL SYMPOSIUM ON PERSIMMON 996- 133 -137 2013
- K. Yonemori; M. Yamada; Zhengrong Luo; S. Kanzaki; Yong Yang; Renzi Wang I INTERNATIONAL SYMPOSIUM ON WILD RELATIVES OF SUBTROPICAL AND TEMPERATE FRUIT AND NUT CROPS 948- 121 -128 2012 [Refereed]
- 三谷宣仁; 河野淳; 山田昌彦; 神崎真哉; 佐藤明彦; 小林省藏; 伴雄介; 上野俊人; 白石美樹夫; 赤木剛士; 辻本誠幸; 米森敬三 園芸学研究 別冊 10- (1) 39 2011/03
- HAYASHI TAKAHIRO; NAKAMURA MINAKO; YOSHIOKA YUMI; NAKAGAWA MASAHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI; SASAOKA MOTONOBU; ICHINOKI MUNEJI; YOSHIMI TAKANORI; SHIMADA TOMOHIRO; TOMI KEN'ICHI; MATSUMURA YASUKI 園芸学研究 別冊 9- (1) 212 2010/03
- K. Yonemori; T. Akagi; S. Kanzaki I INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF FRUIT SPECIES: BIOTECHFRUIT2008 839- 625 -629 2009
- K. Yonemori; S. Kanzaki; C. Honsho; T. Akagi; D. E. Parfitt Advances in Horticultural Science 22- 261 -268 2008/12
- FUMURO M.; UTSUNOMIYA N.; SASAKI K.; SHIMIZU K.; KANZAKI S. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 7- (1) 322 -322 2008/03
- TAKAMATSU Y.; OTSUKA M.; KANZAKI S.; UTSUNOMIYA N. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (2) 153 -153 2007/09
- HASHIMOTO H.; SAKAMOTO A.; KANZAKI S.; UTSUNOMIYA N.; FUMURO M.; SHIMIZU K. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (2) 447 -447 2007/09
- KANZAKI S.; KIMURA M.; YAMADA M.; MITANI N.; AKAGI T.; UTSUNOMIYA N.; YONEMORI K. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (1) 356 -356 2007/03
- KANZAKI S.; HARADA T.; NAKAJIMA A.; SAKO Y.; UTSUNOMIYA N. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (1) 380 -380 2007/03
- WAKISAKA M.; SUGIMURA T.; KANZAKI S. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (1) 95 -95 2007/03
- FUMURO M.; UTSUNOMIYA N.; SASAKI K.; SHIMIZU K.; KANZAKI S. 園芸学研究. 別冊, 園芸学会大会研究発表要旨 6- (1) 376 -376 2007/03
- AKAGI T.; MASUKO T.; KANZAKI S.; MITANI N.; YAMADA M.; YONEMORI K. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 75- (2) 168 -168 2006/09
- Utsunomiya N.; Ide Y.; Yamada K.; Kanzaki S. Japanese journal of tropical agriculture 49- (2) 65 -66 2005/10
- UTSUNOMIYA N.; NAKA S.; KANZAKI S.; SASAKI K.; SHIMIZU K. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 74- (2) 340 -340 2005/10
- Kanzaki S.; Asakura J.; Utsunomiya N. Japanese journal of tropical agriculture 49- (1) 71 -72 2005/03
- Kanzaki S.; Takamori S.; Utsunomiya N. Japanese journal of tropical agriculture 49- (1) 69 -70 2005/03
- TAKAMATSU Y.; OTSUKA M.; KANZAKI S.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 74- (1) 266 -266 2005/03
- KANZAKI S.; MUTO K.; NAKAJIMA A.; SAKO Y.; SASAKI K.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 74- (1) 200 -200 2005/03
- UTSUNOMIYA N.; OKAMOTO S.; NAKA S.; KANZAKI S.; SASAKI K.; SHIMIZU K. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 74- (1) 92 -92 2005/03
- Flower visitors and pests of mango in the hothouse堀川 勇次; 佐々木 勝昭; 宇都宮 直樹; 神崎 真哉; 志水 恒介; 香取 郁夫; 櫻谷 保之 近畿大学農学部紀要 38- 19 -30 2005/03
- K Yonemori; A Ikegami; A Kitajima; A Sato; M Yamada; ZR Luo; Y Yang; RZ Wang; S Kanzaki Proceedings of the Third International Symposium on Persimmon 685- (685) 77 -83 2005
- Horikawa Y.; Sakuratani Y.; Kandori J.; kanzaki S.; Utsunomiya N.; Simizu K.; Sasaki K. Japanese journal of tropical agriculture 48- (2) 89 -90 2004/10
- TAKAMATSU Y.; KUBOTA I.; CYUJYO A.; MATSUI N.; KANZAKI S.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 73- (2) 132 -132 2004/09
- Ueda A.; Takamatsu Y.; Kanzaki S.; Utsunomiya . N. Japanese journal of tropical agriculture 47- (2) 7 -8 2003/09
- Okamoto S.; Takamatsu Y.; Kanzaki S.; Shimizu K.; Sasaki . K.; Utsunomiya N. Japanese journal of tropical agriculture 47- (2) 3 -4 2003/09
- 神崎 真哉 園芸学会雑誌. 別冊, 園芸学会大会研究発表 72- (2) 58 -59 2003/09
- TAKAMATSU Y.; YAMADA S.; YAMAMOTO H.; KANZAKI S.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 72- (2) 131 -131 2003/09
- KANZAKI S.; SATO A.; YAMADA M.; MASUKO T.; UTSUOMIYA N.; YONEMORI K. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 72- (1) 348 -348 2003/04
- WAKISAKA S.; KANZAKI S.; TAKAMATSU Y.; UTSUNOMIYA N. J. Japan. Soc. Hort. Sci. 72- (1) 144 -144 2003/04
- Shimizu K.; Sasaki K.; Takamatsu Y.; Kanzaki S.; Utsunomiya N. Japanese journal of tropical agriculture 47- (1) 43 -44 2003/03
- Ueki K.; Takamatsu Y.; Kanzaki S.; Utsunomiya N. Japanese journal of tropical agriculture 47- (1) 41 -42 2003/03
- K Yonemori; A Ikegami; S Kanzaki; A Sugiura PROCEEDINGS OF THE 2ND INTERNATIONAL PERSIMMON SYMPOSIUM 601- (601) 31 -35 2003
- K Yonemori; A Sugiura; A Sato; M Yamada; S Kanzaki GENETICS AND BREEDING OF TREE FRUITS AND NUTS 622- (622) 189 -203 2003
- WAKISAKA S.; KANZAKI S.; TAKAMATSU Y.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 71- (2) 477 -477 2002/10
- TAKAMATSU Y.; ABE N.; OHYAMA H.; KANZAKI S.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 71- (2) 108 -108 2002/10
- Sawada T.; Takamatsu Y.; Kanzaki K.; Shimizu K.; Sasaki K.; Utsunomiya N. Japanese journal of tropical agriculture 46- (2) 78 -79 2002/09
- Shimizu K.; Sasaki K.; Takamatsu Y.; Kanzaki S.; Utsunomiya N. Japanese journal of tropical agriculture 46- (2) 80 -81 2002/09
- Ueki K.; Takamatsu Y.; Kanzaki S.; Utsunomiya N. Japanese journal of tropical agriculture 46- (2) 90 -91 2002/09
- SATO A.; KANZAKI S.; YONEMORI K.; IKEGAMI A.; YAMADA M.; LUO Z.; WANG R.; YANG Y. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 71- (1) 234 -234 2002/04
- WAKISAKA S.; KANZAKI S.; TAKAMATSU Y.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 71- (1) 68 -68 2002/04
- TAKAMATSU Y.; ABE N.; OHYAMA T.; KANZAKI S.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 71- (1) 83 -83 2002/04
- Utsunomiya N.; Chinenn Y.; Kanzaki S.; Takamatsu Y. Japanese journal of tropical agriculture 46- (1) 25 -26 2002/03
- UTSUNOMIYA N.; KANZAKI S.; TAKAMATSU Y. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 70- (2) 247 -247 2001/09
- TAKAMATSU Y.; KUTSUKAKE Y.; KANZAKI S.; SASAKI K.; TAKEBAYASHI T.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 70- (2) 108 -108 2001/09
- NOSAKA M.; KANZAKI S.; YONEMORI K.; SATO A.; YAMADA M.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 70- (2) 401 -401 2001/09
- KANZAKI S.; KOKUDO K.; IMAMURA R.; SASAKI K.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 70- (1) 190 -190 2001/04
- IKEGAMI A.; YONEMORI K.; SATO A.; YAMADA M.; KANZAKI S.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (2) 311 -311 2000/09
- HONSHO C.; KANZAKI S.; YONEMORI K.; EIADTHONG W.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (2) 291 -291 2000/09
- NOSAKA M.; KANZAKI S.; YONEMORI K.; SATO A.; YAMADA M.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (2) 290 -290 2000/09
- SENTOU A.; KANZAKI S.; TAKAMATSU Y.; UTUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (2) 130 -130 2000/09
- TAJIMA A.; KANZAKI S.; TAKAMATSU Y.; UTSUNOMIYA N. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (2) 194 -194 2000/09
- KANZAKI S.; YONEMORI K.; SATO A.; YAMADA M.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 69- (1) 171 -171 2000/03
- 神崎 真哉; 米森 敬三; 山田 昌彦; 佐藤 明彦; 杉浦 明 園芸学会雑誌. 別冊, 園芸学会大会研究発表 68- (1) 132 -132 1999/04
- Current situation of molecular markers for selecting pollination-constant and non-astringent type from breeding populations of Japanese persimmonYonemori, K; S. Kanzaki; A. Sugiura Proc. 1st Mediterranean Sym. on Persimmon 81 -86 1999
- KANZAKI S.; YONEMORI K.; YAMADA M.; SATO A.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 67- (1) 54 -54 1998/04
- YONEMORI K; NAKAMURA M.; KANZAKI S.; NAKANO R.; TAO R.; SUGIURA A. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 67- (1) 56 -56 1998/04
- EIADTHONG W.; KANZAKI S.; YONEMORI K.; SUGIURA A.; UTSUNOMIYA N.; NAKATSUBO F.; SUBHADRABANDHU S. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 66- (1) 20 -21 1997/03
- S Kanzaki; A Sugiura; K Yonemori; N Utsunomiya; S Subhadrabandhu FIRST INTERNATIONAL PERSIMMON SYMPOSIUM 436- (436) 93 -99 1997
- KANZAKI S.; YONEMORI K.; SUGIURA A.; SUBHADRABANDHU S. 園芸学会雑誌. 別冊, 園芸学会大会研究発表 65- (1) 90 -91 1996/03
Lectures, oral presentations, etc.
- 異なる非辛味化因子を有するトウガラシ(Capsicum chinense)品種における果実の揮発性成分分析 [Not invited]蓮真海; 尾崎早也佳; 大野公輝; 木下万智子; 富研一; 田中義行; 神崎真哉; 神崎真哉; 小枝壮太; 小枝壮太園芸学研究 別冊 2018/09
- 新たに見出した組換え型PepYLCIVの感染性クローンを用いたベンサミアナタバコへの接種 [Not invited]岡部祥子; KESUMAWATI Elly; ZAKARIA Sabaruddin; 神崎真哉; 小枝壮太園芸学研究 別冊 2018/09
- Ty‐3a抵抗性トマト品種におけるTYLCKaVの増殖およびウイルスゲノムのメチル化 [Not invited]藤原郁也; KESUMAWATI Elly; ZAKARIA Sabaruddin; 神崎真哉; 神崎真哉; 小枝壮太; 小枝壮太園芸学研究 別冊 2018/09
- マンゴー‘愛紅’における交互結実栽培の有効性 [Not invited]清水恒介; 巽賢太郎; 神崎真哉; 宇都宮直樹園芸学研究 別冊 2018/09
- マンゴーの花成誘導因子MiFTの発現量および発現時期が花芽形成に及ぼす影響 [Not invited]巽賢太郎; 小枝壮太; 志水恒介; 神崎真哉園芸学研究 別冊 2018/09
- マンゴーの果皮着色に関与する転写制御因子の機能解析 [Not invited]一樋明日香; 小枝壮太; 志水恒介; 神崎真哉園芸学研究 別冊 2018/09
- マンゴー品種間における果実着色機構の差異 [Not invited]神崎真哉; 田中祐太; 一樋明日香; 山本雄介; 小枝壮太; 志水恒介園芸学研究 別冊 2018/03
- インドネシアの園芸作物における黄化葉巻病に関連するベゴモウイルスの同定 [Not invited]KESUMAWATI Elly; 岡部祥子; 本間鹿波; 藤原郁也; ZAKARIA Sabaruddin; 神崎真哉; 小枝壮太園芸学研究 別冊 2018/03
- Ty‐3a抵抗性トマト品種におけるTYLCKaVおよびPepYLCIVの増殖 [Not invited]藤原郁也; KESUMAWATI Elly; ZAKARIA Sabaruddin; 神崎真哉; 小枝壮太園芸学研究 別冊 2018/03
- 交互結実栽培を行ったマンゴー‘愛紅’の収量性 [Not invited]志水恒介; 巽賢太郎; 神崎 真哉; 宇都宮直樹熱帯農業学会 2017/10
- 収穫時期の異なるマンゴー品種間における花成及び結実特性の違い [Not invited]巽賢太郎; 志水恒介; 宇都宮直樹; 神崎真哉熱帯農業研究 2017/10
- マンゴーの果皮着色に関わる転写制御因子の解析 [Not invited]一樋明日香; 神川汐里; 志水恒介; 小枝壮太; 神崎 真哉園芸学会 2017/09
- マンゴー‘Irwin’における花成誘導因子MiFTの発現に関する諸要因の検討 [Not invited]神崎 真哉; 志水恒介; 小枝壮太; 中田昌希; 河村征; 山本拓実; 木下直哉; 宇都宮直樹熱帯農業学会 2016/10
- マンゴー‘愛紅’における剪定時期と着果が次年度における花芽形成に及ぼす影響 [Not invited]志水恒介; 神崎 真哉; 宇都宮直樹熱帯農業学会 2016/10
- マンゴーにおけるモノテルペン合成関連遺伝子の単離 [Not invited]梅津貴子; 白石沙織; 矢森貴之; 小枝壮太; 志水恒介; 神崎 真哉園芸学会 2016/09
- マンゴー‘Irwin’果皮における UFGT 様遺伝子の単離とその発現解析 [Not invited]神川汐里; 一樋明日香; 野口成美; 小枝壮太; 志水恒介; 神崎 真哉園芸学会 2016/09
- ベトナム北部山間農村に分布するカキ属植物の探索 [Not invited]KANZAKI SHIN'YA; UTSUNOMIYA NAOKI; SATO AKIHIKO; KITAJIMA AKIRA; HOA TRAN THI; YONEMORI KEIZO園芸学研究 別冊 2015/03
- マンゴー果皮におけるMYB転写因子の単離と発現解析 [Not invited]HYODO YOSHIHIRO; KANZAKI SHIN'YA; SHIMIZU KOSUKE; FUMURO MASAHIKO; UTSUNOMIYA NAOKI園芸学研究 別冊 2014/09
- カンキツ類果実における香気成分の同定と品種間差異 [Not invited]KAMATANI MASAAKI; TOMI KEN'ICHI; KANZAKI SHIN'YA; SHIMIZU KOSUKE; FUMURO MASAHIKO; HAYASHI TAKAHIRO; UTSUNOMIYA NAOKI園芸学研究 別冊 2014/03
- マンゴーの果実および葉に含まれる香気成分の同定と品種間差異 [Not invited]OKAZAKI TOSHIKAZU; KANZAKI SHIN'YA; TOMI KEN'ICHI; HAYASHI TAKAHIRO; SHIMIZU KOSUKE; FUMURO MASAHIKO; UTSUNOMIYA NAOKI園芸学研究 別冊 2013/09
- マンゴーの萌芽時における花成関連遺伝子の発現解析 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; HONSHO CHITOSE; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI園芸学研究 別冊 2011/09
- マンゴー果実における香気成分の品種間差異 [Not invited]TSUJI TOSHIHIKO; NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; FUMURO MASAHIKO; UTSUNOMIYA NAOKI熱帯農業研究 2011/03
- 晩生品種‘Sensation’を用いたマンゴーの抑制栽培技術の検討 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; FUMURO MASAHIKO; UTSUNOMIYA NAOKI熱帯農業研究 2011/03
- カキの甘渋性識別SCARマーカーを利用した完全甘ガキの選抜 [Not invited]MITANI NORIHITO; KONO ATSUSHI; YAMADA MASAHIKO; KANZAKI SHIN'YA; SATO AKIHIKO; KOBAYASHI SHOZO; BAN YUSUKE; UENO TOSHIHITO; SHIRAISHI MIKIO; AKAGI TSUYOSHI; TSUJIMOTO TOMOYUKI; YONEMORI KEIZO園芸学研究 別冊 2011/03
- マンゴー‘Irwin’の花成関連遺伝子の発現に及ぼす温度の影響 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; HONSHO CHITOSE; FUMURO MASAHIKO; UTSUNOMIYA NAOKI園芸学研究 別冊 2011/03
- マンゴーの花成誘導期間中におけるジベレリン代謝関連遺伝子の発現解析 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; FUMURO MASAHIKO; UTSUNOMIYA NAOKI熱帯農業研究 2010/10
- カキの遺伝学的解析におけるマメガキの有用性 [Not invited]TSUJIMOTO TAKAYUKI; AKAGI TAKEJI; KANZAKI SHIN'YA; YONEMORI KEIZO園芸学研究 別冊 2010/09
- マンゴーの果肉崩壊症に及ぼす嫌気呼吸活性の影響 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI熱帯農業研究 2010/03
- 光質と窒素施肥量がハーブの香気ケモタイプに及ぼす影響 [Not invited]HAYASHI TAKAHIRO; NAKAMURA MINAKO; YOSHIOKA YUMI; NAKAGAWA MASAHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI; SASAOKA MOTONOBU; ICHINOKI MUNEJI; YOSHIMI TAKANORI; SHIMADA TOMOHIRO; TOMI KEN'ICHI; MATSUMURA YASUKI園芸学研究 別冊 2010/03
- Effect of sugar concentrations, temperature and light on the anthocyanin formation in the flesh of blood orange [Not invited]UTSUNOMIYA NAOKI; SAKAMOTO AKI; KANZAKI SHIN'YA園芸学研究 別冊 2009/09 秋田 園芸学会平成21年度秋季大会
ブラッドオレンジの果肉におけるアントシアニン形成に及ぼすショ糖濃度、温度、光の影響を調査し、温度がその形成に強い影響を及ぼしていることを明らかにした。 - イロハカエデのSSRマーカーの開発 [Not invited]KANZAKI SHIN'YA; INOUE KOICHI; UTSUNOMIYA NAOKI; YANO MASAYOSHI園芸学研究 別冊 2009/09
- 1‐MCPおよびプロピオン酸カルシウム処理がマンゴー‘愛紅’果実の軟化に及ぼす影響 [Not invited]NAKAGAWA MASAHIRO; SHIMIZU KOSUKE; KANZAKI SHIN'YA; FUMURO MASAHIKO; UTSUNOMIYA NAOKI熱帯農業研究 2009/03
- マンゴー‘アーウィン’および‘愛紅’の取り木発根向上のための処理方法 [Not invited]FUMURO MASAHIKO; UTSUNOMIYA NAOKI; SASAKI KATSUAKI; SHIMIZU KOSUKE; KANZAKI SHIN'YA園芸学研究 別冊 2009/03
- カキの落葉時葉色の品種・系統間差異と紅葉生産に適した品種・系統の選抜 [Not invited]YAMADA MASAHIKO; KOGA YOSUKE; HIGA KOJI; YOKOYAMA TAKAMITSU; KOBAYASHI SHOZO; KONO ATSUSHI; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI園芸学研究 別冊 2009/03
- マンゴー‘アーウィン’,‘愛紅’および台湾在来種の取り木発根に及ぼすIBAとNAAの影響 [Not invited]FUMURO MASAHIKO; UTSUNOMIYA NAOKI; SASAKI KATSUAKI; SHIMIZU KOSUKE; KANZAKI SHIN'YA園芸学研究 別冊 2008/09 園芸学会
- ドラゴンフルーツにおける葉状茎の生長に及ぼす植物生長調節物質の影響 [Not invited]FUMURO MASAHIKO; UTSUNOMIYA NAOKI; SASAKI KATSUAKI; SHIMIZU KOSUKE; KANZAKI SHIN'YA園芸学研究 別冊 2008/03 園芸学会
- Effect of temperature on the anthocyanin formation in blood orange fruit after harvesting [Not invited]HASHIMOTO H; SAKAMOTO A; KANZAKI S; UTSUNOMIYA N; FUMURO M; SHIMIZU K園芸学研究. 別冊, 園芸学会大会研究発表要旨 2007/09
- Effect of temperature on the development of flower and vegetative buds in Japanese apricot 'Nankho' [Not invited]TAKAMATSU Y; OTSUKA M; KANZAKI S; UTSUNOMIYA N園芸学研究. 別冊, 園芸学会大会研究発表要旨 2007/09
- KANZAKI S; HARADA T; NAKAJIMA A; SAKO Y; UTSUNOMIYA N園芸学研究. 別冊, 園芸学会大会研究発表要旨 2007/03
- KANZAKI S; KIMURA M; YAMADA M; MITANI N; AKAGI T; UTSUNOMIYA N; YONEMORI K園芸学研究. 別冊, 園芸学会大会研究発表要旨 2007/03
- Effect of Grafting Season and Presence of Leaves in Stock on Growth of Grafted Nursery Plant of Persimmon [Not invited]WAKISAKA M; SUGIMURA T; KANZAKI S園芸学研究. 別冊, 園芸学会大会研究発表要旨 2007/03
- ドラゴンフルーツにおける果実形質間の相関関係およびGA3とCPPUが果実生長に及ぼす影響 [Not invited]FUMURO MASAHIKO; UTSUNOMIYA NAOKI; SASAKI KATSUAKI; SHIMIZU KOSUKE; KANZAKI SHIN'YA園芸学研究 別冊 2007/03
- カキ‘黒熊’由来のBC1集団における果実脱渋性の分離とRFLPマーカーによる完全甘ガキの選抜 [Not invited]KANZAKI SHIN'YA; KIMURA MIYUKI; YAMADA MASAHIKO; MITANI NOBUHITO; AKAGI TSUYOSHI; UTSUNOMIYA NAOKI; YONEMORI KEIZO園芸学研究 別冊 2007/03
- ドラゴンフルーツにおける果実形質間の相関関係およびGA3とCPPUが果実生長に及ぼす影響 [Not invited]文室 政彦; 宇都宮 直樹; 神崎 真哉; 佐々木 勝昭; 志水 恒介園芸学会 2007 園芸学会
- マンゴー‘Irwin’果実への低温処理が果実着色に及ぼす影響 [Not invited]YOSHIDA MARIKO; KANZAKI SHIN'YA; SHIMIZU KOSUKE; FUMURO MASAHIKO; SASAKI KATSUAKI; UTSUNOMIYA NAOKI熱帯農業 2007
- Possibility for identification of AST-locus in D. kaki by chromosome walking of D. lotus [Not invited]AKAGI T; MASUKO T; KANZAKI S; MITANI N; YAMADA M; YONEMORI K園芸学会雑誌. 別冊, 園芸学会大会研究発表 2006/09
- マイクロサテライトマーカーによる完全甘ガキ品種の識別 [Not invited]KANZAKI SHIN'YA; MAEDA YASUNORI; YAMADA MASAHIKO; MITANI NORIHITO; IWAMOTO KAZUHIKO; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2006/03
- RFLPマーカーによるカキの甘渋性の識別 [Not invited]KANZAKI SHIN'YA; YONEMORI KEIZO; SUGIURA AKIRA; SATO AKIHIKO; YAMADA MASAHIKO果樹研究成果情報 2006/02
- Utsunomiya N; Ide Y; Yamada K; Kanzaki SJapanese journal of tropical agriculture 2005/10
- ムラサキクダモノトケイソウ果実の着色に及ぼす光と温度の影響 [Not invited]UTSUNOMIYA NAOKI; IDE YUKIKO; YAMADA TAKAKO; KAMIZAKI SHIN'YA熱帯農業 2005/10
- UTSUNOMIYA N; NAKA S; KANZAKI S; SASAKI K; SHIMIZU K園芸学会雑誌. 別冊, 園芸学会大会研究発表 2005/10
- AFLP法によるサンショウ(Zanthoxylum piperitum)の系統解析 [Not invited]KANZAKI SHIN'YA; MUTO KENTA; NAKAJIMA AKIHIRO; SAKO YOSHINAGA; SASAKI KATSUAKI; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2005/04
- ウメ‘南高’の花芽の発達と葉芽の萌芽に及ぼす温度の影響 [Not invited]TAKAMATSU YOSHIHIRO; OTSUKA MAYO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2005/04
- マンゴー‘アーウィン’果実における遮光処理がアントシアニン形成に及ぼす影響 [Not invited]UTSUNOMIYA NAOKI; OKAMOTO SATOSHI; NAKA SEN'ICHIRO; KANZAKI SHIN'YA; SASAKI KATSUAKI; SHIMIZU KOSUKE園芸学会雑誌 別冊 2005/04
- 36. Polymorphism analysis by AFLP method among seedlings from some passion fruit species [Not invited]Kanzaki S; Asakura J; Utsunomiya NJapanese journal of tropical agriculture 2005/03
- Kanzaki S; Takamori S; Utsunomiya NJapanese journal of tropical agriculture 2005/03
- Effect of temperature on the development of flower buds and budbreak of vegetative buds in Japanese apricot 'Nankho' [Not invited]TAKAMATSU Y; OTSUKA M; KANZAKI S; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2005/03
- UTSUNOMIYA N; OKAMOTO S; NAKA S; KANZAKI S; SASAKI K; SHIMIZU K園芸学会雑誌. 別冊, 園芸学会大会研究発表 2005/03
- Horikawa Y; Sakuratani Y; Kandori J; kanzaki S; Utsunomiya N; Simizu K; Sasaki KJapanese journal of tropical agriculture 2004/10
- Effect of temperatures on flower bud development and flowering during different growth stages in 'Nanko' Japanese apricot [Not invited]TAKAMATSU Y; KUBOTA I; CYUJYO A; MATSUI N; KANZAKI S; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2004/09
- Okamoto S; Takamatsu Y; Kanzaki S; Shimizu K; Sasaki . K; Utsunomiya NJapanese journal of tropical agriculture 2003/09
- In vitroで得られたマンゴスチン幼植物体の生長に及ぼす諸要因の影響と接ぎ木の可能性 [Not invited]UEDA AKIO; TAKAMATSU YOSHIHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI熱帯農業 2003/09
- Bud dormancy and flower bud development as affected by high temperature in Japanese apricot 'Nanko' [Not invited]TAKAMATSU Y; YAMADA S; YAMAMOTO H; KANZAKI S; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2003/09
- KANZAKI SHIN'YA園芸学会雑誌 別冊 2003/09
- Verification of the marker-assisted selection system for PCNA phenotype of Japanese persimmon [Not invited]KANZAKI S; SATO A; YAMADA M; MASUKO T; UTSUOMIYA N; YONEMORI K園芸学会雑誌. 別冊, 園芸学会大会研究発表 2003/04
- WAKISAKA S; KANZAKI S; TAKAMATSU Y; UTSUNOMIYA NJ. Japan. Soc. Hort. Sci. 2003/04
- Effects of the inflorescence thinning on the yield and profit in ' Irwin' mango trees grown under plastic house condition [Not invited]Shimizu K; Sasaki K; Takamatsu Y; Kanzaki S; Utsunomiya NJapanese journal of tropical agriculture 2003/03
- Ueki K; Takamatsu Y; Kanzaki S; Utsunomiya NJapanese journal of tropical agriculture 2003/03
- ウメ‘南高’の休眠現象と花芽の発達に及ぼす低温の影響 [Not invited]TAKAMATSU YOSHIHIRO; ABE NOBUHISA; OYAMA HIROSHI; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2002/10
- WAKISAKA S; KANZAKI S; TAKAMATSU Y; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2002/10
- UEKI KANJI; TAKAMATSU YOSHIHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI熱帯農業 2002/09 日本熱帯農業学会第92回講演会(奈良)
パッションフルーツ数種類を用いて、カルス誘導と個体再生およびプロトプラスト培養の可能性について検討した。 - Ueki K; Takamatsu Y; Kanzaki S; Utsunomiya NJapanese journal of tropical agriculture 2002/09
- Shimizu K; Sasaki K; Takamatsu Y; Kanzaki S; Utsunomiya NJapanese journal of tropical agriculture 2002/09
- Sawada T; Takamatsu Y; Kanzaki K; Shimizu K; Sasaki K; Utsunomiya NJapanese journal of tropical agriculture 2002/09
- ハウス栽培マンゴーにおける冬季のせん定処理が花穂形成と果実生長に及ぼす影響 [Not invited]SHIMIZU KOSUKE; SASAKI KATSUAKI; TAKAMATSU YOSHIHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI熱帯農業 2002/09
- 花粉親の違いがマンゴーの着果及びはい発育に及ぼす影響 [Not invited]SAWADA TOSHIHARU; TAKAMATSU YOSHIHIRO; KANZAKI SHIN'YA; SHIMIZU KOSUKE; SASAKI KATSUAKI; UTSUNOMIYA NAOKI熱帯農業 2002/09
- TAKAMATSU YOSHIHIRO; ABE NOBUHISA; OYAMA TAKUSHI; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2002/04 園芸学会平成 14 年度春季大会 (千葉)
ウメ南高の鉢植え樹を用いて、 日長が葉芽および花芽の発達に及ぼす影響を検討し、 あまり影響を及ぼさないことを明らかにした。 - Isolation of SSR markers in persimmon [Not invited]WAKISAKA S; KANZAKI S; TAKAMATSU Y; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2002/04
- SATO A; KANZAKI S; YONEMORI K; IKEGAMI A; YAMADA M; LUO Z; WANG R; YANG Y園芸学会雑誌. 別冊, 園芸学会大会研究発表 2002/04
- In vitroでのマンゴスチン幼植物体からの個体再生 [Not invited]UTSUNOMIYA NAOKI; CHINEN YOKO; KANZAKI SHIN'YA; TAKAMATSU YOSHIHIRO熱帯農業 2002/03
- 熱帯果樹における花器の形態的特色 [Not invited]UTSUNOMIYA NAOKI; KANZAKI SHIN'YA; INOUE HIROAKI; SUBHADRABANDHU S; SUGIURA AKIRA熱帯農業 2001/10
- AFLP法によるマンゴスチンの多様性解析の有効性 [Not invited]WAKISAKA SATOSHI; TAKAMATSU YOSHIHIRO; KANZAKI SHIN'YA; UTSUNOMIYA NAOKI熱帯農業 2001/10
- Possibility of inter specific hybrids with crossing in Passifloras, especially in purple passionfruit [Not invited]UTSUNOMIYA NAOKI; KANZAKI SHIN'YA; TAKAMATSU YOSHIHIRO園芸学会雑誌 別冊 2001/09 園芸学会平成 13 年度秋季大会 (弘前)
ムラサキクダモノトケイソウを種子親としたパッションフルーツ類の交配の可能性と交配個体の生育特性について論じた。 - PCRを利用した完全甘ガキ個体の簡易判別法の可能性 [Not invited]NOSAKA MITSUNORI; KAMINZA SHIN'YA; YONEMORI KEIZO; SATO AKIHIKO; YAMADA MASAHIKO; SUGIURA AKIRA園芸学会雑誌 別冊 2001/09
- 走査型電子顕微鏡による4種の核果類の花芽および葉芽の観察 [Not invited]TAKAMATSU YOSHIHIRO; KUTSUKAKE YOKO; KANZAKI SHIN'YA; SASAKI KATSUAKI; TAKEBAYASHI TERUO; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2001/09
- PCR method for selecting pollination-constant and non-astringent (PCNA) type persimmon [Not invited]NOSAKA M; KANZAKI S; YONEMORI K; SATO A; YAMADA M; SUGIURA A園芸学会雑誌. 別冊, 園芸学会大会研究発表 2001/09
- Scanning Electron Microscopy of flower and leaf bud development of four stone fruits species [Not invited]TAKAMATSU Y; KUTSUKAKE Y; KANZAKI S; SASAKI K; TAKEBAYASHI T; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2001/09
- マンゴー‘金こう’由来の実生の特性 [Not invited]KANZAKI SHIN'YA; KOKUDO KAZUTAKA; IMAMURA RUI; SASAKI KATSUAKI; UTSUNOMIYA NAOKI園芸学会雑誌 別冊 2001/04
- Kanzaki Shinya; Yonemori Keizo; Sato Akihiko; Yamada Masahiko; Sugiura AkiraJournal of the Japanese Society for Horticultural Science 2000/11The genetic relationships among 19 pollination-constant and non-astringent (PCNA) cultivars and 14 non-PCNA cultivars of persimmon (Diospyros kaki Thunb.), including one Chinese PCNA cultivar 'Luo Tian Tian Shi', were analyzed by comparing 138 AFLP markers. A phylogenetic tree constructed based on the similarity indices of these AFLP markers indicated a close relationship among Japanese PCNA cultivars, but a more distant relationship with the Chinese PCNA cultivar 'Luo Tian Tian Shi'. The close relationship between PCNA cultivars native to Gifu prefecture was distinct, indicating that these cultivars developed from crosses among restricted sources in this region. The cultivars 'Gosho', 'Hana-gosho', 'Oo-gosho', and 'Yamato-gosho' showed a close relationship with some non-PCNA cultivars.
- 「アーウィン」マンゴー樹における冬季の切り戻しせん定処理が開花・結実に及ぼす影響 [Not invited]SASAKI KATSUAKI; SHIMIZU KOSUKE; TAKAMATSU YOSHIHIRO; KANZAKI KEN'YA; UTSUNOMIYA NAOKI熱帯農業 2000/10
- HONSHO C; KANZAKI S; YONEMORI K; EIADTHONG W; SUGIURA A園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/09
- In vitro shoot formation from young fruit of fig [Not invited]SENTOU A; KANZAKI S; TAKAMATSU Y; UTUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/09
- Genetic relationships of persimmon (Diospyros kaki Thunb.) cultivars revealed by AFLP analysis [Not invited]NOSAKA M; KANZAKI S; YONEMORI K; SATO A; YAMADA M; SUGIURA A園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/09
- Inheritance of the trait for natural astringency-loss in the progeny of 'Luo Tian Tian Shi' [Not invited]IKEGAMI A; YONEMORI K; SATO A; YAMADA M; KANZAKI S; SUGIURA A園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/09
- The effect of sludge compost as nitrogen fertilizer on the fruit growth and quality in potted grown strawberry [Not invited]TAJIMA A; KANZAKI S; TAKAMATSU Y; UTSUNOMIYA N園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/09
- KANZAKI S; YONEMORI K; SATO A; YAMADA M; SUGIURA A園芸学会雑誌. 別冊, 園芸学会大会研究発表 2000/03
- 完全甘ガキの早期選抜のための分子マーカーの同定 [Not invited]神崎 真哉; 米森 敬三; 山田 昌彦; 佐藤 明彦; 杉浦 明園芸学会雑誌. 別冊, 園芸学会大会研究発表 1999/04
- EIADTHONG W; KANZAKI SHIN'YA; YONEMORI KEIZO; SUGIURA AKIRA; UTSUNOMIYA NAOKI; NAKATSUBO FUMIAKI; SUBHADRABANDHU S園芸学会雑誌 別冊 1997/03
- KANZAKI SHIN'YA; YONEMORI KEIZO; SUGIURA AKIRA; SUBHADRABANDHU S園芸学会雑誌 別冊 1996/03
Research Themes
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2017/04 -2020/03Author : Kanzaki ShinyaThis study was conducted to analyze the differences in the control mechanism of flower induction between mango cultivars and to elucidate the factors that induce flowering other than low temperature, and to obtain useful knowledge for new cultivation method and stable production of mangoes. The results showed that MiFT gene expression was involved in the induction of flowering induced by low temperature in other cultivars as well as in 'Irwin', but MiFT was not involved in summer flowering. In addition, 'Irwin' was found to flower in summer, which is thought to be required low temperature for floral induction. We plan to investigate the mechanism of summer flowering through comprehensive transcriptome analysis.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2016/04 -2020/03Author : Yonemori KeizoThe AST locus controlling persimmon astringency was determined by comparative mapping with Diospyros lotus, a diploid relative of D. kaki, as a reference. The syntenic region of AST locus was 915-kb in physical map of D. lotus. In addition, transcriptome and coexpression network analyses between PCNA and non-PCNA progenies revealed that AST gene affects a few transcription factors centered in the PA regulatory network. On the other hand, transcriptome analysis of the gene conferring non-astringent trait in Chinese-type PCNA persimmon indicated that the genes conferring sugar transporter, flavonoid glucosyltransferase, and flavonoid/phenylplopaloid O-methyltransferase may associate with this trait. Finally, a new innovative strategy was considered for PCNA persimmon breeding including Chinese-type PCNA persimmons.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2013/04 -2017/03Author : Yonemori KeizoThe accuracy of AFLP markers linked to the locus of natural astringency-loss in Chinese-type PCNA persimmon were confirmed and then, the markers were used as probes for fosmid/BAC library constructed from Diospyros lotus, which is a close diploid relative of D. kaki, for constructing contig to identify the locus of natural astringency-loss. However, the results showed that the fosmid/BAC library is hard to use directly for identifying the locus probably due to the difference of genome structure between D. kaki and D. lotus. On the other hand, RNA-seq analysis using segregated progeny between Chinese-type PCNA and non-PCNA indicated that the genes related to transportation in the cell were differently expressed among them, as well as the genes related to flavonoid biosynthesis pathway.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2012/04 -2017/03Author : Yonemori KeizoThe survey was conducted in unfinished Northern mountainous area in Vietnam for obtaining new species related to D. kaki and we found several trees which bear pubescent fruits. However, these trees seem to be the same species which we collected previously in Vietnam, so that there are no new species from this survey. In addition, although we conducted resurvey of Diospyros spp. in germplasm repository of China and the campus of Kasetsart University in Thailand, we could not find new species closely related to D. kaki. The phylogenetic studies from DNA sequences of ITS and matK regions in “ye-mao-shi”, which was assumed to closely relate to D. kaki from morphological characteristics, is indicated that this species should be included with D. glandulosa which assumed to be ancestor of D. kaki, due to matK analysis, not due to ITS analysis. This contradiction of the results from each analysis should be corrected by the further analysis in the future.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2013 -2015Author : KANZAKI ShinyaThis study was conducted to analyze the expression pattern of MiFT, which is a main factor for floral induction in Mango, to investigate the effect of endogenous and exogenous factors on floral induction and expression of MiFT, and to analyze the floral related genes other than MiFT. It was revealed that experiencing cool temperature below 15℃ for 130 hours was enough to increase the expression of MiFT in mango. It was also revealed that expression of MiFT in young leaves was lower than old leaves. In addition, some genes that showed down- or up-regulation by the floral-inductive cool temperature in mango leaves were obtained. These genes are under investigation.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2010/04 -2013/03Author : YONEMORI Keizo; SATO Akihiko; YAMANE Hisay0; KANZAKI ShinyaFosmid library was constructed by a diploid relative, Diospyros lotus, of D. kaki (hexaploid) for isolation of AST gene which confers astringency trait in persimmon. After screening seed clone from fosmid library by using a probe of AST-linked marker, fosmid contig was developed by further screening of the library using end-sequences of the clone. In addition, BAC library of D. lotus was constructed and BAC contig was also developed. Each contig seemed to contain AST locus there, so that we were approaching very near to isolate the AST gene. Furthermore, this BAC library was shown to be effective to isolate the CPCNA gene which confers the trait of non-astringency in Chinese-type non-astringent persimmon. Using the BAC library, isolation of CPCNA gene may also be possible.
- Ministry of Education, Culture, Sports, Science and Technology:Grants-in-Aid for Scientific Research(基盤研究(C))Date (from‐to) : 2010 -2012Author : Shinya KANZAKI; Chitose HONSHOTo develop an practical method for determining the male parent of mango seedlings, high-polymorphic SSR markers were selected and multiplex PCR method was developed. Progenies obtained from open pollinated 'Irwin' trees and 'Beni-Keitt' trees in a plastic house were used for determining male parents. The results showed that cross-pollinated seedlings were major in both cultivars. In addition, Myb transcription factor associated with anthocyanin biosynthesis were isolated from mango and its structure was analyzed.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2008 -2011Author : YONEMORI Keizo; KITAJIMA Akira; TAO Ryutaro; KANZAKI Shinya; YAMANE HisayoThere is a report to show that Diospyros kaki is closely related to D. lotus, D. oleifera, and D. glandulosa. In addition to these three species, we started to find some Diospyros spp. having close relationships to D. kaki in the area of South China and Northern mountain regions of Thailand and Vietnam where D. kaki (cultivated kaki) thought to be originated. Due to our exploration, we found several species of Diospyros, among which we clarified that some species have morphologically very close features to D. kaki. “Ye-mao-shi” from China and “Hong Coc” from Vietnam are two species in our interests. Especially, “Ye-mao-shi” is the most interesting species because of its very close morphology to D. kaki. When the distribution of “Ye-mao-shi” was investigated by specimen survey in botanical gardens in China, it clearly showed that this species aredistributed only in Yunnan province. We also showed a possibility that “Ye-mao-shi” is diploid, as the same as D. lotus, D. oleifera, and D. glandulosa. We need further investigations using molecular analyses to clarify the phylogenetic position of this species to D. kaki and to consider the involvement of this species for appearance of cultivated kaki.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2008 -2010Author : KATAOKA Ikuo; KANZAKI ShinyaPloidy variants of wild A.arguta specifically occurred in Japan, were characterized. A wide range of ploidy variance from diploid to octaploid was observed. Geographic distribution of the variants was determined showing the localization of the higher ploidy population in Tohoku area. Morphology, fruit composition and reproductive character of the ploidy variants were determined. Cross compatibility of the ploidy variants with kiwifruit (A.deliciosa) was observed. The characteristics of the cross seedlings showed the potential of the variants as breeding materials.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2007 -2009Author : YONEMORI Keizo; YAMADA Masahiko; SATO Akihiko; TAO Ryutaro; YAMANE Hisayo; KANNZAKI ShinyaThe gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.
- 文部科学省:科学研究費補助金(若手研究(B))Date (from‐to) : 2005 -2007Author : 神崎 真哉近年、日本でマンゴーのハウス栽培が拡大しつつあり、高級果物としての需要が高まっている。日本では主に‘Irwin'が栽培されており、成熟期に鮮やかな赤色を呈するのが特徴である。温帯果樹のリンゴやブドウでは、高温によりアントシアニン合成が阻害されることが示されており、30℃を超える条件で栽培すると、果実着色は不良となる。一方、マンゴー‘Irwin'は40℃を超える高温下で栽培されているにも関わらず、着色への影響はあまり見られない。本研究では、マンゴーの着色と気温との関係を明らかにするために、低温処理がマンゴーの着色に及ぼす影響について調査した。低温処理(果実周辺温度を20〜25℃に保つ)した果実では、アントシアニン含量は対照区(果実周辺気温25〜45℃)と差がなかったが、クロロフィル含量の減少が抑制され、果皮はくすんだような赤色になった。一方、アントシアニン合成系(PAL, CHS, ANS)の遺伝子発現は、低温処理によって増加していた。夜間のみ低温処理を行った果実でも、アントシアニン含量は対照区と同程度であり、低温によるアントシアニン合成の促進は見られなかった。それに対し、遮光処理を行うと、アントシアニン含量は著しく抑制され、また、夜間低温処理と遮光を組み合わせると、アントシアニン蓄積とクロロフィル分解が共に抑制されることが示された。マンゴー果皮におけるアントシアニン合成系遺伝子群の発現量はアントシアニン含量と相関が低く、低温や遮光が遺伝子発現に及ぼす影響は明確でない。以上の結果から、マンゴー‘Irwin'の果皮におけるアントシアニン蓄積は、30℃以上の高温でもほとんど阻害されることなく、気温の影響は光の影響と比較して小さいことが明らかとなった。20〜25℃程度の低温は、成熟を遅延させ、クロロフィルの分解を妨げることから、赤色を鮮やかに発色させるためには適さないと考えられた。
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2004 -2006Author : YONEMORI Keizo; YAMADA Masahiko; TAO Ryutaro; KITAJIMA Akira; KANZAKI Shinya; HABU TsuyoshiThe analysis was performed to identify key genes for regulating tannin accumulation into fruits of pollination constant non-astringent (PCNA)-type of persimmon originated in Japan and China. For this purpose, we conducted the following three experiments: 1)We constructed genomic library from closely related diploid species, D. lotus, and a seed clone was screened from the library with a probe tightly linked to the trait of natural astringency-loss of Japanese-type PCNA fruits. Then, we constructed ca. 300 kbp contig of D. lotus. Due to the sequence analysis of this contig, we found that the sequences are very homologous to those of D. kaki and it can use for exploring the gene of persimmon (D. kaki). In addition, primer sets designed from the sequences of this contig were able to be applicable to distinguish Japanese-type PCNA from others. We could make a universal primer set to select PCNA persimmon. 2)We conducted the expression analysis of mRNA to identify the genes involved in tannin accumulation of persimmon. For this purpose, suppression subtractive hybridization (SSH) analysis was performed between astringency-removed fruits with ethanol treatment on the tree and non-treated fruits. According to this analysis, we were able to obtain several candidates for the genes involved in tannin accumulation into the fruits. 3)As for investigation of a dominant gene conferring the trait of Chinese-type PCNA, we looked for molecular markers by AFLP method combined with bulked segregant analysis (BSA) using progenies derived from a Chinese PCNA 'Luo Tian Tian Shi'. By this analysis, we found three markers closely linked to the Chinese-PCNA trait. Using a marker among them, we screened genomic library of `Luo Tian Tian Shi' and obtained a genomic clone as the seed for constructing contig for the survey of the gene.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2003 -2006Author : YONEMORI Keizo; NAMATSUBO Fumiaki; KITAJIMA Akira; YAMADA Masahiko; TAO Ryutaro; KANZAKI ShinyaThe purpose of this study is 1) to clarify whether pollination constant non-astringent (PCNA) persimmons exist in China except for 'Luo Tian Tian Shi', in addition to clarify an uniqueness of `Luo Tian Tian Shi', and 2) to survey ancestral species of persimmon (Diospyros kaki Thunb.). For this purpose, we conducted the following investigations: 1)We constructed survey trips to Luotian county in Hubei province, where 'Luo Tian Tian Shi' was found. As the results, we were able to find new PCNA cultivars named `Qiuyan Tian Shi', 'Baogai Tian Shi', and 'Xiaoguo Tian Shi'. In addition, we were able to investigate the original tree of `Luo Tian Tian Shi', which showed us `Luo Tian Tian Shi' originated from a seedling. 2)The uniqueness of PCNA trait in `Luo Tian Tian Shi' was clarified to investigate the F_1 progenies derived from crossings between 'Luo Tian Tian Shi' and Japanese PCNA or non-PCNA cultivars. In F_1 progenies from all crossings, PCNA and non-PCNA individuals were segregated at the ratio of 1:1. It means that the trait of Chinese PCNA is dominant. It is quite different from recessive trait of Japanese PCNA. 3)As for consideration of ancestral species of persimmon, the survey of specimens was conducted in Botanical Garden in Kunming, Wuhan, and Beijing. We found an interesting species named "ye mao shi" classified as Diospyros kaki var. sylvestris. This species has a pubescent fruit surface, while wild persimmon (D. kaki) named "ye shi" has glabrous fruits. The morphological characteristic of "ya mao shi" was very similar to D. galndulosa, which is supposed to be a progenitor of D. kaki. We also could find the trees of this species in the forest of Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Science.
- 文部科学省:科学研究費補助金(若手研究(B))Date (from‐to) : 2002 -2003Author : 神崎 真哉本研究では完全甘ガキの自然脱渋性を支配する遺伝子の探索を進めるために1)ゲノムライブラリーの作成と甘渋性識別マーカー周辺領域の単離、2)甘渋性に連鎖した新規マーカーの探索、を試みた。以下、その概要を記す。1)ゲノムライブラリーの作成と甘渋性識別マーカー周辺領域の単離カキ品種'次郎'(完全甘ガキ)由来のゲノムライブラリーを作成し、甘渋性識別マーカーを用いてスクリーニングを行った結果、約27kbのインサートを持つクローンが単離された。このインサートについて甘渋性識別マーカー周辺領域の簡単な制限酵素マップを作成した。現在、単離されたゲノム断片について、より詳細な調査を行っている。2)甘渋性に連鎖した新規マーカーの探索これまでに同定されている甘渋性に連鎖したマーカーのみでは、遺伝子の探索を進めるにあたり十分な情報が得られないため、ゲノミックサブトラクション法を用いて新規マーカーの探索を試みた。サブトラクション後のPCR増幅断片をいくつか単離し、プローブに用いてRFLP分析を行っているが、現在までに甘渋性に連鎖した新規のマーカーは得られていない。今後は異なる制限酵素を用いたゲノミックサブトラクションを試みていく予定である。
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 2001 -2003Author : YONEMORI Keizo; SATO Akihiko; TAO Ryutaro; YAMADA Masahiko; KANZAKI ShinyaSo far, it was thought that no non-astringent-type persimmons exist in China. However, a pollination constant non-astringent (PCNA) persimmon was recently found in Lou Tian Tian province of China, and this PCNA-type cultivar, 'Luo Tian Tian Shi', have showed very unique trait for astringency. When 'Luo Tian Tian Shi' was crossed wish a Japanese PCNA cultivar, several offspring bore astringent fruits. In order to confirm this phenomenon, tannin cell size that is a good index to distinguish PCNA from non-PCNA was determined. As the result, we were able to confirm that non-PCNA offsping by a cross between 'Luo Tian Tian Shi' and a Japanese PCNA cultivar were yielded wish a relatively high rate in the offspring. We also clarified that the chemical properties of tannins in the fruit is quite different between 'Luo Tian Tian Shi' and Japanese PCNA cultivars. Tannins from 'Luo Tian Tian Shi' showed higher molecular weight and faster coagulation reaction with acetaldehyde than in Japanese PCNA cultivars. The chemical properties of tannins of 'Luo Tian Tian Shi' were resemble to those of non-PCNA cultivars. Furthermore, gene expressions involved in flavonoid biosynthesis, which is the first step for tannin biosynthesis, were also different between 'Luo Tian Tian Shi' and Japanese PCNA cultivars. As a conclusion, we could demonstrated a unique trait of astringency in 'Luo Tian Tian Shi', which was thought to be due to a difference of gene expressions involved in flavonoid biosynthesis.
- Japan Society for the Promotion of Science:Grants-in-Aid for Scientific ResearchDate (from‐to) : 1997 -2000Author : YONEMORI Keizo; TETSUMURA Takuya; TAO Ryutaro; YAMADA Masuhiko; KANZAKI Shinya; SATO AkihikoThe pollination-constant and non-astringent (PCNA)-type fruit is the most desirable persimmon for fresh consumption, and new PCNA-type cultivars with good fruit quality have been awaited worldwide. However, the inheritance of PCNA-type is qualitative and the PCNA-type is recessive to non-PCNA (PVNA, PVA, and PCA) types. Molecular marker closely linked to the trait of natural astringency-loss is a powerful tool for the breeding project. So, we tried to establish it by the following experiments to make the breeding process more efficient. 1. Bulked segregant analysis (BSA) of 6 PCNA-type and 10 non-PCNA-type offspring in breeding population was performed by AFLP method using 128 primer combinations. As a result, 4 potential AFLP markers were detected for discriminating PCNA- and non-PCNA types. 2. The most reliable marker among 4 potential AFLP markers was selected and sequenced. When this marker was used as a probe for Southern analysis after genomic DNA was digested HindlII, all progenies examined could be distinguished between PCNA-type and non-PCNA type by the existence or absence of 6.5kb or 8.0 kb band. Furthermore, segregation rate of these bands indicated that persimmon shows polysomic inheritance. Persimmon seemed to be an auto- or autoallo-hexaploid. 3. When Southern analysis using AFLP maker as a probe was examined to existing cultivars, PCNA cultivars could be distinguished from non-PCNA culitvars with 100% accuracy. This result indicates a high reliability of this analysis for selecting PCNA-type among wider breeding populations. 4. The sequencing analysis of two RFLP markers (6.5 kb and 8.0 kb) for characterizing them and making primers for PCR analysis for easier detection of these makers was not completely successful. We could sequence 8.0 kb band but not 6.5 kb band. So, further study was needed to design PCR primers for more easier method than Southern analysis to distinguish PCNA-type from non-PCNA-type.
- 熱帯果樹類の遺伝・育種その他の研究制度
- カキの甘渋判別マーカーの探索その他の研究制度
- Breeding and Genetics for Some Tropical FruitsCooperative Research
- Survey of specific markers for PCNA nature of Japanese PersimmonCooperative Research