KINDAI UNIVERSITY


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MATSUKAWA Tetsuya

Profile

FacultyDepartment of Biotechnological Science / Graduate School of Biology-Oriented Science and Technology / Experimental Farms
PositionAssociate Professor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/904-matsukawa-tetsuya.html
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Mail
Last Updated :2020/09/01

Education and Career

Education

  •  - 2002 , Kyoto University
  •  - 1998 , Kyoto University, Faculty of Agriculture

Research Activities

Research Areas

  • Life sciences, Bioorganic chemistry

Research Interests

  • chemotaxonomy

Published Papers

  • Pancreatic lipase inhibitory activity of Citrus unshiu leaf extract, MATSUKAWA Tetsuya, Natural Product Communications, Natural Product Communications, September, 1 - 5, Sep. 2019 , Refereed
  • Promotion of lipopeptide antibiotic production by Bacillus sp. IA in the presence of rice husk biochar., Ebe S, Ohike T, Matsukawa T, Okanami M, Kajiyama S, Ano T, Journal of Pesticide Science, Journal of Pesticide Science, 44(1), 33 - 40, Feb. 2019 , Refereed
  • Bioogical potential of Streptomyces sp. AR10 producing albocycline isolated from soil around ant nest, Ohike T, Matsukawa T, Okanami M, Kajiyama S, Ano T, Journal of Agricultural Science, Journal of Agricultural Science, 10(3), 54 - 61, Mar. 2018 , Refereed
  • In vitro and in vivo assay for assessment of the biological control potential of Streptomyces sp. KT, Ohike T, Maeda M, Matsukawa T, Okanami M, Kajiyama S, Ano T, Journal of Plant Studies, Journal of Plant Studies, 7(1), 10 - 18, Mar. 2018 , Refereed
  • Metabolic fingerprinting of Citrus cultivars and related genera using HPLC and multivariate analysis, MATSUKAWA Tetsuya, Nito Nobumasa, Journal of Plant Studies, Journal of Plant Studies, 7(1), 1 - 9, Mar. 2018 , Refereed
  • Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract, Itoh K, Murata K, Sakaguchi N, Akai K, Yamaji T, Shimizu K, Isaki K, Matsukawa T, Kajiyama S, Fumuro M, Iijima M, Matsuda H, Jounal of Plant Studies, Jounal of Plant Studies, 6(2), 102 - 107, Sep. 2017 , Refereed
  • Influence of Force-Ripening Condition on Anthocyanin Accumulation and Content of Components of 'Tsuyuakane' (Prunus salicina × Prunus mume) Fruit, 大江 孝明, 竹中 正好, 根来 圭一, 北村 祐人, 松川 哲也, 三谷 隆彦, 赤木 知裕, 古屋 挙幸, 岡室 美絵子, 土田 靖久, 園芸学研究 = Horticultural research (Japan), 園芸学研究 = Horticultural research (Japan), 15(4), 439 - 444, Oct. 15 2016
  • A Pancreatic Lipase Inhibitory Activity by Mango (Mangifera indica) Leaf Methanolic Extract, Kimihisa Itoh, Kazuya Murata, Yuta Nakagaki, Ayaka Shimizu, Yusuke Takata, Kohsuke Shimizu, Tetsuya Matsukawa, Shin'ichiro Kajiyama, Masahiko Fumuro, Morio Iijima, Hideaki Matsuda, Journal of Plant Studies, Journal of Plant Studies, 5(2), 72 - 78, Sep. 2016 , Refereed
  • Characterization of a Medicinal Perilla Native to Wakayama Prefecture, and Growth Regulation by Using Monochromatic Lighting, Horibata Akira, Yata Kiyoshi, Matsukawa Tetsuya, Journal of crop research, Journal of crop research, (58), 43 - 50, 2013
    Summary:We found one line of Perilla herb with good smell, as a local genetic resource that may have been cultivated in the Kinokawa plain since ancient times. In this study, firstly, we revealed the morphological and aromatic characteristic of this line by comparison with that of four varieties of Perilla frutescens. Since the leaves of this plant were dark purplish-red color on both surfaces without crepe-like undulations, and this plant contained a notable amount of perillaldehyde and limonene, it was suggested that this line of perilla was one of the varieties belonging to the medicinal red perilla, P. frutescens var. crispa f. purpurea. Secondly, to grow up this resource as a local special product, we have begun to develop the cultivation method for year round production and open up new uses. Supplemental lighting by blue LEDs to the perilla plants which were grown under the natural daylight dreadfully inhibited the flower formation, and allowed the continuous differentiation of young leaves wealthy in perillaldehyde. This cultivation method is useful to inhibit flower formation during short-day period and to improve the functional component content in this perilla herb.
  • Germplasm Conservation of Indigenous Citrus, Acta Horticulturae 760, Acta Horticulturae 760, Jul. 2007
  • Metabolomic analysis of primary metabolites in citrus leaf during defense responses, Tomonori Asai, Tetsuya Matsukawa, Shin'ichiro Kajiyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 376 - 381, Mar. 2017 , Refereed
    Summary:Mechanical damage is one of the unavoidable environmental stresses to plant growth and development. Plants induce a variety of reactions which defend against natural enemies and/or heal the wounded sites. Jasmonic acid (JA) and salicylic acid (SA), defense-related plant hormones, are well known to be involved in induction of defense reactions and play important roles as signal molecules. However, defense related metabolites are so numerous and diverse that roles of individual compounds are still to be elucidated. In this report, we carried out a comprehensive analysis of metabolic changes during wound response in citrus plants which are one of the most commercially important fruit tree families. Changes in amino acid, sugar, and organic acid profiles in leaves were surveyed after wounding, JA and SA treatments using gas chromatography mass spectrometry (GC/MS) in seven citrus species, Citrus sinensis, Citrus limon, Citrus paradisi, Citrus unshiu, Citrus kinokuni, Citrus grandis, and Citrus hassaku. GC/MS data were applied to multivariate analyses including hierarchical cluster analysis (HCA), primary component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) to extract stress-related compounds. HCA showed the amino acid cluster including phenylalanine and tryptophan, suggesting that amino acids in this cluster are concertedly regulated during responses against treatments. OPLS-DA exhibited that tryptophan was accumulated after wounding and JA treatments in all species tested, while serine was down regulated. Our results suggest that tryptophan and serine are common biomarker candidates in citrus plants for wound stress. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
  • Quantitative trait loci responsible for the difference in gamma-oryzanol content in brown rice between japonica-type and indica-type rice cultivars, Tsuneo Kato, Tetsuya Matsukawa, Akira Horibata, PLANT PRODUCTION SCIENCE, PLANT PRODUCTION SCIENCE, 20(4), 459 - 466, 2017 , Refereed
    Summary:gamma-Oryzanol is a main oleophilic component in rice bran oil and has been well recognized as a good dietary supplement for human health, as well as having uses in industrial materials. japonica-type rice cultivars generally showed significantly higher contents of total gamma-oryzanol in brown rice compared with indica-type cultivars, although within-group variation was significant. The objective of this study was to explore quantitative trait loci (QTLs) responsible for the difference in the gamma-oryzanol content between japonica-type and indica-type rice cultivars, using recombinant inbred lines (RILs), backcross inbred lines (BILs), and corresponding chromosome segment substitution lines (CSSLs) derived from crosses between japonica-type and indica-type. Results from RILs and BILs showed that eight QTLs were detected with R-2 from.09 to.16. Nine candidate regions for QTL were also suggested from corresponding CSSLs. These QTLs from RILs and BILs and the candidate regions from CSSLs were not overlapped, although one QTLs was mapped near the boundaries of the respective candidate region. At four QTLs and three candidate regions, alleles or segments from japonica-type caused higher contents than those from indica-type. On the other hand, at the other four QTLs and six candidate regions, alleles or segments from indica-type caused higher contents than those from japonica-type, which is a reverse result to the parental differences. This result strongly suggested that alleles with increasing effects on.-oryzanol content could be accumulated not only from japonica-type but also from indica-type, leading to a potential for increase in gamma-oryzanol content in future breeding programs.
  • Isolation and characterization of wound-induced compounds from the leaves of Citrus hassaku, Tomonori Asai, Tetsuya Matsukawa, Atsushi Ishihara, Shin'ichiro Kajiyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(2), 208 - 212, Aug. 2016 , Refereed
    Summary:Citrus plants are world widely cultivated as horticultural tree crops, and nowadays their pharmacological activities have been well studied. Since research of defense responses in citrus plants have been mainly focused on the post-harvested fruits because of their commercial importance, defense mechanisms during their developmental stages have not been well understood. In the present study, two wound-induced compounds were isolated from leaves of Citrus hassalcu, and their structures were elucidated by high-resolution electron spray ionization mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) analyses. One of these compounds was identified as a known flavanone, hesperetin. The other was characterized as a novel furofuran lignan, and was named `biscitrusnin-A'. Their antimicrobial activities were also evaluated. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
  • Metabolic changes in Citrus leaf volatiles in response to environmental stress, Tomonori Asai, Tetsuya Matsukawa, Shin'ichiro Kajiyamal, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(2), 235 - 241, Feb. 2016 , Refereed
    Summary:Citrus plants are well known as a rich source of VOCs, and several have important roles in defense responses. However, how VOCs are regulated in response to environmental stress is not yet well understood. In this study, we investigated dynamic changes of VOCs present in leaves of seven Citrus species (Citrus sinensis, C. limon, C. paradisi, C. unshiu, C. kinokuni, C grandis, and C hassaku) in response to mechanical wounding, jasmonic acid (JA), and salicylic acid (SA) as determined by gas chromatography/mass spectrometric analysis followed by multivariate analysis (principal component analysis, PCA, and orthogonal partial least squares-discriminant analysis, OPLS-DA). PCA and OPLS-DA suggested that changes in VOC profiles against stress stimuli were much diverse among Citrus species. OPLS-DA showed that C6 volatiles, such as hexanal and trans-2-hexenal, were induced in response to JA and SA stimuli in C sinensis and C. grandis, while the other VOCs were decreased under all tested stress conditions. alpha-Farnesene was induced in all species except C. hassaku after wounding or JA treatment. In addition, alpha-farnesene was also induced in response to SA stimuli in C. unshiu and C kinokuni. Therefore these volatiles can be candidates of the common stress biomarkers in Citrus. Our results will give a new insight into defense mechanisms in Citrus species. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
  • A Novel Cropping Method for Production of High Functioning Crops by Utilizing On-site Solar Energy, Akira Horibata, Tetsuya Matsukawa, Takuji Itoh, Toshiaki Watanabe, Toshiroh Matsumoto, 2013 ISES SOLAR WORLD CONGRESS, 2013 ISES SOLAR WORLD CONGRESS, 57, 1502 - 1507, 2014 , Refereed
    Summary:The utilization efficiency of solar energy through photosynthesis by crops is only a few percent at most. Therefore, most solar energy in the field (on-site) remains unused. Plants are, however, able to use certain types of monochromatic light as an environmental signal, and they exhibit various physiological responses to even small amounts of the light. In particular, the significant induction of secondary metabolite production by blue light is well documented. Here, we propose a novel cropping method where unused solar energy is captured on-site by solar cells and converted to monochromatic light by using LEDs. The crops are then irradiated to stimulate enhanced production of secondary metabolites, thus improving crop function without the need for an external energy source. In this study, the effect of blue light on the production of functional secondary metabolites was evaluated in perilla (Perilla frutescens var. crispa f. purpurea), a traditional medicinal crop in Japan. Perilla leaves contain several functional substances such as perillaldehyde, rosmarinic acid, and anthocyanins, which have notable stomachic, antipyretic, detoxification, sedation, and diuretic efficacy. Plants were grown under natural daylight supplemented with blue light irradiation (460 nm, approx. 10 mu mol/m(2)/s, 24 h,) for 108 days before harvest. Control plants were grown without supplemental lighting. The content ratio of perillaldehyde in plants grown with blue light was 60% higher at harvest than in the controls. Moreover, in the vegetative growth stage, the perillaldehyde content increased significantly after only three days of blue light irradiation. These findings suggest that the present approach will benefit agricultural practices in developing countries through more efficient usage of on-site solar energy. (C) 2014 The Authors. Published by Elsevier Ltd.
  • Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye, Masayuki Sue, Kana Yamazaki, Shunsuke Yajima, Taiji Nomura, Tetsuya Matsukawa, Hajime Iwamura, Toru Miyamoto, PLANT PHYSIOLOGY, PLANT PHYSIOLOGY, 141(4), 1237 - 1247, Aug. 2006 , Refereed
    Summary:The wheat ( Triticum aestivum) and rye ( Secale cereale) beta-D-glucosidases hydrolyze hydroxamic acid-glucose conjugates, exist as different types of isozyme, and function as oligomers. In this study, three cDNAs encoding beta-D-glucosidases (TaGlu1a, TaGlu1b, and TaGlu1c) were isolated from young wheat shoots. Although the TaGlu1s share very high sequence homology, the mRNA level of Taglu1c was much lower than the other two genes in 48- and 96-h-old wheat shoots. The expression ratio of each gene was different between two wheat cultivars. Recombinant TaGlu1b expressed in Escherichia coli was electrophoretically distinct from TaGlu1a and TaGlu1c. Furthermore, coexpression of TaGlu1a and TaGlu1b gave seven bands on a native-PAGE gel, indicating the formation of both homo- and heterohexamers. One distinctive property of the wheat and rye glucosidases is that they function as hexamers but lose activity when dissociated into smaller oligomers or monomers. The crystal structure of hexameric TaGlu1b was determined at a resolution of 1.8 angstrom. The N-terminal region was located at the dimer-dimer interface and plays a crucial role in hexamer formation. Mutational analyses revealed that the aromatic side chain at position 378, which is located at the entrance to the catalytic center, plays an important role in substrate binding. Additionally, serine-464 and leucine-465 of TaGlu1a were shown to be critical in the relative specificity for DIMBOA-glucose (2-O-b-D-glucopyranosyl-4- hydroxy-7-methoxy-1,4-benzoxazin-3-one) over DIBOA-glucose (7-demethoxy-DIMBOA-glucose).
  • Metabolism of avenanthramide phytoalexins in oats, Y Okazaki, T Isobe, Y Iwata, T Matsukawa, F Matsuda, H Miyagawa, A Ishihara, T Nishioka, H Iwamura, PLANT JOURNAL, PLANT JOURNAL, 39(4), 560 - 572, Aug. 2004 , Refereed
    Summary:Oat leaves produce phytoalexins, avenanthramides, in response to infection by pathogens or treatment with elicitors. The metabolism of avenanthramides was investigated using low molecular weight, partially deacetylated chitin as an elicitor. When oat leaf segments are floated on the elicitor solution, avenanthramides accumulate in the solution. The transfer of elicited oat leaves to solutions containing stable-isotope-labeled avenanthramides resulted in a rapid decrease in the labeled avenanthramides, suggesting the metabolism of avenanthramides. The rate of decrease was enhanced by elicitor treatment, and was dependent on the species of avenanthramides, with avenanthramide B decreasing most rapidly. The rates of biosynthesis and metabolism of avenanthramides A and B were measured using a model of isotope-labeling dynamics. Avenanthramide B was found to be more actively biosynthesized and metabolized than avenanthramide A. Radiolabeled avenanthramide B was incorporated into the cell wall fraction and 99% of incorporated activity was released by alkaline treatment. Gel filtration indicated that high-molecular-weight compounds derived from avenanthramide B were released by alkaline treatment. The decrease in stable-isotope-labeled avenanthramides was suppressed by catalase, salicylhydroxamic acid, and sodium ascorbate, suggesting the involvement of peroxidase in the metabolism. Consistent with this, peroxidase activity that accepts avenanthramide B as a substrate was induced in apoplastic fractions by elicitor treatment. The appearance of multiple basic isoperoxidases was observed by activity staining with 3-amino-9-ethylcarbazole coupled with isoelectric focusing of proteins from elicitor-treated leaves. These findings suggest that accumulated avenanthramides are further metabolized in apoplasts in oat leaves by inducible isoperoxidases.
  • Differential induction of chorismate mutase isoforms by elicitors in oat leaves, T Matsukawa, A Ishihara, H Iwamura, PLANT PHYSIOLOGY AND BIOCHEMISTRY, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 40(9), 795 - 802, Sep. 2002 , Refereed
    Summary:Avenanthramides, a series of substituted cinnamic acid amides with anthranilate, are phytoalexins in oats (Avena sativa L.). The precursors of avenanthramides, cinnamate and anthranilate, are biosynthesized via the shikimate pathway that branches at chorismate. Chorismate mutase (CM, EC 5.4.99.5) is the first enzyme on the branch that provides the cinnamate part of avenanthramides. The induction of CM was investigated in primary oat leaves using oligo-N-acetylchitooligosaccharides as elicitors. The CM activity started to increase 6 h after elicitation, and reached a maximum by 9 h, being around twice as large as that in control leaves. Among the oligo-Nacetylchitooligosaccharides tested, tetra-, penta-, and hexasaccharides effectively induced the CM activity in a dose-dependent manner. The activity was separated into two major peaks on anion exchange chromatography with Mono Q, indicating that at least two CM isoforms are present in oat leaves. A comparison of elution profiles of CM activity in intact and elicitor-treated leaves revealed that only one CM isoform is responsive to the elicitor. Two CM isoforms in oat leaves were partially purified and characterized. Both CM isoforms were insensitive to L-phenylalanine, L-tyrosine, L-tryptophan, and caffeate. The fractionation of oat cells indicated that both CM isoforms localized in plastids. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
  • Induction of anthranilate synthase activity by elicitors in oats, T Matsukawa, A Ishihara, H Iwamura, ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 57(1-2), 121 - 128, Jan. 2002 , Refereed
    Summary:Oat phytoalexins, avenanthramides, are a series of substituted hydroxycinnamic acid amides with anthranilate. The anthranilate in avenanthramides is biosynthesized by anthranilate synthase (AS, EC 4.1.3.27). Induction of anthranilate synthase activity was investigated in oat leaves treated with oligo-N-acetylchitooligosaccharide elicitors. AS activity increased transiently, peaking 6 h after the elicitation. The induction of activity was dependent on the concentration and the degree of polymerization of the oligo-N-acetylchitooligosaccharide elicitor. These findings indicate that the induction is part of a concerted biochemical change required for avenanthramide production. The elicitor-inducible AS activity was strongly inhibited by L-tryptophan and its analogues including 5-methyl-DL-tryptophan, and 5- and 6-fluoro-DL-tryptophan, while the activity was not affected by D-tryptophan. The accumulation of avenanthramide A was also inhibited by treatment of elicited leaves with these AS inhibitors, indicating that a feedback-sensitive AS is responsible for the avenanthramide production. In elicited leaves, the content of free anthranilate remained at a steady, low level during avenanthramide production. Moreover, administration of anthranilate to elicited oat leaves resulted in an enhanced avenanthramide accumulation. AS may play a role as a rate-limiting enzyme in the biosynthesis of avenanthramides.
  • Occurrence of avenanthramides and hydroxycinnamoyl-CoA : hydroxyanthranilate N-hydroxycinnamoyltransferase activity in oat seeds, T Matsukawa, T Isobe, A Ishihara, H Iwamura, ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, 55(1-2), 30 - 36, Jan. 2000 , Refereed
    Summary:Oat phytoalexins, avenanthramides, occur as constitutive components in seeds. The amounts of each avenanthramide were analyzed. The composition of avenanthramides in dry seeds was different from that in elicitor-treated leaves. In seeds, avenanthramide C was most abundant with an amount two times larger than that of avenanthramide A or B. On the other hand, avenanthramide A was the major component in elicitor-treated leaves. The total amount of avenanthramides in seeds increased 2.5 times during imbibition for 48 h although the composition did not change. The hydroxycinnamoyl-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase (HHT, EC 2.3.1.-) activity, which is responsible for the final condensation step in the avenanthramide biosynthesis, was detected in dry seeds. The activity was localized in endosperm and scutellum, and slightly increased during 48-h imbibition. The enzyme was partially purified by anion exchange chromatography from both dry seeds and elicitor-treated leaves. The activity was separated into two peaks by chromatography, indicating that HHT consists of at least two isoforms. The substrate specificities of HHT isoforms from seeds were different from each other.
  • Occurrence of avenanthramide and hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroxycinnamoyltransferase activity in oat seeds, Zeitshrift fuf Naturforshung, Zeitshrift fuf Naturforshung, 55c, 30-36, 2000

Books etc

  • Citrus Pathology, Matsukawa T, Asai T, Kajiyama S, Joint author, Metabolic Changes during Defense Responses against Wound Stresses in Citrus Plants, InTech,   2017
  • Amino Acids in Higher Plants, Ishihara A, Matsukawa T, Nomura T, Sue M, Oikawa A, Okazaki Y, Tebayashi S, Joint author, Involvement of tryptophan-pathway-derived secondary metabolism in the defence responses of grasses, CABI,   2015

Conference Activities & Talks

  • Metabolic profiling of secondary metabolites in phorbol ester containing and deficient Jatropha curcas seeds, Kosuke Matsukubo, Tetsuya Matsukawa, Shinichiro Kajiyama, American Chemical Society fall 2019 National Meeting,   2019 08
  • Purification and identification of a putative sperm chemoattractant in the liverwort Marchantia polymorpha L, Yumiko Yamasaki, Miho Takemura, Tetsuya Matsukawa, Katsuyuki Yamato, Shinichiro Kajiyama, American Chemical Society fall 2019 National Meeting,   2019 08
  • Analysis of chemical diversity in Lilium japonicum population using floral scent composition, Mizuki Fujisawa, Tetsuya Matsukawa, Toshiharu Akino, Shigeru Arai, Yoshihiro Takikawa, Shinichiro Kajiyama, American Chemical Society fall 2019 National Meeting,   2019 08
  • Metabolic profiling of leaf secondary metabolites in Japanese citrus cultivars under stress conditions, Matsukawa T, Kajiyama S, American Chemical Society fall 2019 National Meeting,   2019 08
  • Metabolomic analysis of secondary metabolites in kinokuni (C. kinokuni) and satsuma (C. unshiu) mandarin leaves during defense responses, Matsukawa T, Kajiyama S, International Research Symposium on Engineering and technology,   2019 07
  • Metabolic profiling of secondary metabolites in citrus leaves under various stress conditions, Isaki K, Matsukawa T, Kajiyama S, Plant Biology 2017,   2017 06
  • Wound inducible antimicrobial compounds I Citrus hassaku leaves., Matsukawa T, Asai T, Kajiyama S, Pacifichem2015,   2015 12
  • Efficacy of Citrus plants on lifestyle diseases: Anti-pbesity effect of Citurs unshiu leaf., Ito K, Shimizu K, Horikawa Y, Tomohiro N, Tsumoto T, Hashizme J, Fujita T, Fumuro M, Utsunomiya N, Takada Y, Matsukawa T, Kajiyama S, Nakagaki Y, Shimizu A, Murata K, Matsuda H, Pacifichem2015,   2015 12
  • Comprehensive analysis of wound induced metabolic change in citrus plants, Asai T, Matsukawa T, Ishihara A, Kajiyama S, 13th IUPAC International Congress of Pesticide Chemistry,   2014 08
  • Potential application of oligotrophic bacteria as biocontrol agents., Ohike T, Matsukawa T, Okanami M, Kajiyama S, Ano T, 13th IUPAC International Congress of Pesticide Chemistry,   2014 08
  • Isolation of phorbol ester degrading microbes for Jatropha bio-diesel production., International Union of Microbial Societies 2011,   2011 09 , International Union of Microbial Societies 2011
  • Germplasm Conservation of Indigenous Citrus Species, The 27th International Horticultural Congress,   2006 08 , The 27th International Horticultural Congress
  • Accumulation of elicitor-inducible proteins in oat leaves, Yamamoto Y, Shinohara M, Matsukawa T, Ishihara A, Iwamura H, 11th IUPAC International Congress of PesticideChemistry,   2006 08
  • Germplasm Conservation of Citrus Species Indigenous to Japan, The 10th International Congress of SABRAO,   2005 08 , The 10th International Congress of SABRAO

Misc

  • Antimicrobial activities by the floral scents of the Japanese bamboo lily Lilium japonicum, the symbolic flowers offered to the deity at the Saikusa Festival, 花﨑 直史, 岩佐 郁, 藤澤 瑞希, 高橋 康樹, 柳川 綾, 梶山 慎一郎, 瀧川 義浩, 松川 哲也, 荒井 滋, 秋野 順治, Aroma research = アロマリサーチ : journal of aroma science technology and safety, 20, 2, 137, 143,   2019 , http://ci.nii.ac.jp/naid/40021932839
  • Past and Current trends in research of Lilium japnicum, sasayuri, 瀧川 義浩, 田中 普祟, 藤澤 瑞希, 松川 哲也, 梶山 慎一郎, Memoirs of Institute of Advanced Technology, Kinki University = 近畿大学先端技術総合研究所紀要, 23, 11, 22,   2018 03 , http://ci.nii.ac.jp/naid/120006469827
    Summary:本論では、希少植物となっているササユリの研究が現在どのような状況となっているのか、そして今後どのようなことができるのか、について記載した。Sasa yurri(Lilium japonicum)is widely recognized as the domestic species of lily in Japan. In the present paper,we described past and current reserch situation of the rare plant"Sasa yuri".
  • Protection and current situation of rare plant "Sasa yuri" in satoyama, 瀧川 義浩, 松川 哲也, 宮下 実, 梶山 慎一郎, Memoirs of Institute of Advanced Technology, Kinki University, 22, 35, 40,   2017 03 , http://ci.nii.ac.jp/naid/120006332227
    Summary:本論では里山におけるササユリの自生環境、個体数の減少の要因および近畿大学 先端技術総合研究所(研究所)と生物理工学部で実施している活動や研究について記載した。Sasa yuri(Lilium japnicum)is a wild lily in japan and also known as an index plant for evaluating a natural environment of satoyama. In the present paper, we described current situation and protection of the rare plant "Sasa yuri ".
  • Germplasms of Red Perilla Native to Wakayama Prefecture, Japan, Horibata Akira, Matsukawa Tetsuya, Journal of Crop Research, 62, 0, 11, 17,   2017 , http://ci.nii.ac.jp/naid/130006267911
    Summary:A native variety of red perilla (<i>Perilla frutescens</i> (L.) Britton var. <i>crispa</i> (Thunb.) H. Deane) with an extremely fine fragrance had been cultivated in the Kinokawa river basin, Wakayama Prefecture, Japan, since ancient times up to about 10 years ago. We hope to promote this germplasm as a regional specialty product; thus, it is necessary to clarify the superiority of the germplasm to other commercial red perilla varieties with similar characteristics. In this study, we compared the morphological characteristics and functional substance content of two germplasms native to Wakayama with those of three commercial varieties of aromatic red perilla and the common variety chirimen-jiso (red perilla with frilly leaves). The morphological characteristics showed that the native Wakayama germplasms are classified as aka-jiso (red perilla with smooth leaves), whereas all three aromatic varieties are classified as chirimen-jiso. Quantitative analysis of the aromatic substances perillaldehyde and limonene showed that native Wakayama germplasms contain more than twice the amount of aromatic substances compared with the aromatic varieties. Thus, the native Wakayama germplasms should be commercially superior. There were no differences between the cultivars in the content of the water-soluble polyphenols rosmarinic acid and caffeic acid, which are the most important functional substances in perilla, in the fresh leaves, although there were greater differences in the dried leaves. This result suggests that the biosynthesis of water-soluble polyphenols continued as the leaves dried.
  • 2P-155 Isolation of antifungal active substance from actinomycetes, Uetsuki Yoshihiro, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010009242
  • 3P-274 Volatile flavor components of Lillium japonicum and their antimicrobial activity, Takahashi Koki, Matsukawa Tetsuya, Takikawa Yoshihiro, Arai Shigeru, Akino Toshiharu, Kajiyama Shin'ichiro, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010009689
  • 3P-101 Evaluation of Trichoderma atroviride HNT-01 as biocontrol agent, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Yamamoto Shigeki, Hirose Yoichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906783
  • 3P-100 Development of biocontrol agents from actinomycetes, Uetsuki Yoshihiro, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906782
  • 2P-232 Analysis of metabolic changes in volatile compounds in Citrus leaves against environmental stress, Asai Tomonori, Matsukawa Tetsuya, Kajiyama Shin'ichiro, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906616
  • 〈Original Papers〉 Evaluation of tumor promoting activity of Jatropha curcas seed extracts by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay., 松川 哲也, 正田 将大, 玉置 恵子, 村上 ひかる, 梶山 慎一, 近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 33, 9, 16,   2014 03 01 , http://ci.nii.ac.jp/naid/120005735156
    Summary:[要旨]トウダイグサ科の落葉低木であるJatropha curcasは, 種子に豊富な中性脂質を含み, 荒廃地でも生育可能であるため次世代バイオディーゼル原料植物として有望視されている。しかし一方で, 本植物の種子には発がんプロモーターであるphorbol ester類を含んでおり, 生産者や消費者の健康に与える負の影響が懸念されている。本研究ではJ. curcas 種子抽出物の発がんプロモーション活性を, マウスを用いた二段階発がん試験およびEBV早期抗原誘導活性を指標としたアッセイ系により評価した。その結果, J. curcas種子抽出物の発がんプロモーション活性は, 発がんプロモーション活性を有する代表的なホルポールエステルであるphorbol-12-myristate-13-acetate(PMA)より低いことが見いだされた。また,種子抽出物はPMAの発がん活性を抑制することが示された。これらの結果から, J. curcas種子抽出物には化学発がんを抑制する物質が含まれることが示唆された。 [Abstract] Jatr•opha eurcas, a tropical plant belonging to the family of Euphorbiaceac, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content in seed kernel. The seed, however, contains phorbol esters which exhibit tumor-promoting activity, and thus negative impact of these compounds on health of producers or consumers should be concerned_ In this study, tumor-promoting activity of I cur eas seed extracts was evaluated by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay. Our results showed that tumor-promoting activity of J. curcas seed extracts was significantly lower than phorbol-l2-myristate-1 3-acetate (PMA), which is well known as a potent tumor promotor. In addition, J. cur eas seed extracts were revealed to suppress the tumor-promoting activity ofPMA. These results suggest that./ curcas seed extracts contain some suppressor of tumorgenesis.
  • 〈Original Papers〉 Evaluation of tumor promoting activity of Jatropha curcas seed extracts by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay., 松川 哲也, 正田 将大, 玉置 恵子, 村上 ひかる, 梶山 慎一, 近畿大学生物理工学部紀要, 33, 9, 16,   2014 03 , http://ci.nii.ac.jp/naid/120005423580
    Summary:[要旨]トウダイグサ科の落葉低木であるJatropha curcasは, 種子に豊富な中性脂質を含み, 荒廃地でも生育可能であるため次世代バイオディーゼル原料植物として有望視されている。しかし一方で, 本植物の種子には発がんプロモーターであるphorbol ester類を含んでおり, 生産者や消費者の健康に与える負の影響が懸念されている。本研究ではJ. curcas 種子抽出物の発がんプロモーション活性を, マウスを用いた二段階発がん試験およびEBV早期抗原誘導活性を指標としたアッセイ系により評価した。その結果, J. curcas種子抽出物の発がんプロモーション活性は, 発がんプロモーション活性を有する代表的なホルポールエステルであるphorbol-12-myristate-13-acetate(PMA)より低いことが見いだされた。また,種子抽出物はPMAの発がん活性を抑制することが示された。これらの結果から, J. curcas種子抽出物には化学発がんを抑制する物質が含まれることが示唆された。 [Abstract] Jatr•opha eurcas, a tropical plant belonging to the family of Euphorbiaceac, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content in seed kernel. The seed, however, contains phorbol esters which exhibit tumor-promoting activity, and thus negative impact of these compounds on health of producers or consumers should be concerned_ In this study, tumor-promoting activity of I cur eas seed extracts was evaluated by the two-stage mouse skin tumorgenesis assay and Epstein-Barr virus early antigen induction assay. Our results showed that tumor-promoting activity of J. curcas seed extracts was significantly lower than phorbol-l2-myristate-1 3-acetate (PMA), which is well known as a potent tumor promotor. In addition, J. cur eas seed extracts were revealed to suppress the tumor-promoting activity ofPMA. These results suggest that./ curcas seed extracts contain some suppressor of tumorgenesis.
  • Toxic Metabolites in a Bio-diesel Source Plant, Jatropha Curcas and its Detoxification, MATSUKAWA Tetsuya, KAJIYAMA Shin'ichiro, Journal of Smart Processing, 3, 5, 258, 261,   2014 , 10.7791/jspmee.3.258, http://ci.nii.ac.jp/naid/130005091324
  • 1P-206 Production of antifungal lipopeptide iturin A by glycerol as BDF by-product, Ohike Tatsuya, Hashigami Kohei, Ishida Yuko, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737586
  • 2P-229 Tumor promoting activity of crude phorbol ester fraction from Jatropha curcas, Shoda Masahiro, Tamaki Keiko, Murakami Hikaru, Matsukawa Tetsuya, Kajiyama Shin'ichiro, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737895
  • 〈Original Papers〉The Effect of the Blue LED Supplemental-lighting on Growth and the Content of Functional Chemical Components of the Native Medicinal Perilla (Perilla frutescens), 'Kishu-zairai', 堀端 章, 松川 哲也, 矢田 清, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 28, 13, 20,   2011 09 01 , http://ci.nii.ac.jp/naid/120005735129
    Summary:[要旨] 本研究は、機能性成分含有量の高い'紀州在来薬用紫蘇' の自然光併用型植物工場での高効率生産を目的とする。自然光下(ガラスハウス内)で栽培している'紀州在来薬用紫蘇'および'赤ちりめんしそ'に青色LEDの光を24時間連続照射し、草丈、主茎節数および機能性成分の一種であるペリルアルデヒドの含有量の変化を調査した。青色光補光による草丈の減少と主茎節数の増加が観察されたが、これらの変化は同時に観察された青色光補光による花成阻害に起因すると考えられた。一方、収穫された葉におけるペリルアルデヒド含有量は青色光照射によって有意に増加した。 [Abstract] Two cultivars of perilla, 'Kishu-zairai' (Perilla frutescens var. crispa f. purpurea, red s mooth 1 eat) and 'Aka-chirimen-shiso' (Perilla frutescens var. crispa f. crispa, red wrinkled leaf), were grown in a greenhouse and were lighted continuously by blue LEDs (460nm, approximately 10μmol/m^2/s). As a result, it was revealed that the blue supplemental-lighting reduced the plant height and increased the number of nodes on main stem in both kind of perilla. These two phenomena were thought to be associated with the inhibition of flower formation by the blue supplemental-lighting. While, the content of perillaldehyde (a kind of functional chemical component) in harvested leaves was increased by the blue supplemental-lighting.本研究は日本学術振興会科学研究費補助金22658077および近畿大学生物理工学部戦略的研究No.09-IV-1, 2010の助成を受けた.
  • 〈Original Papers〉The Effect of the Blue LED Supplemental-lighting on Growth and the Content of Functional Chemical Components of the Native Medicinal Perilla (Perilla frutescens), 'Kishu-zairai', 堀端 章, 松川 哲也, 矢田 清, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 28, 13, 20,   2011 09 , http://ci.nii.ac.jp/naid/120003460255
    Summary:本研究は日本学術振興会科学研究費補助金22658077および近畿大学生物理工学部戦略的研究No.09-IV-1, 2010の助成を受けた.[要旨] 本研究は、機能性成分含有量の高い'紀州在来薬用紫蘇' の自然光併用型植物工場での高効率生産を目的とする。自然光下(ガラスハウス内)で栽培している'紀州在来薬用紫蘇'および'赤ちりめんしそ'に青色LEDの光を24時間連続照射し、草丈、主茎節数および機能性成分の一種であるペリルアルデヒドの含有量の変化を調査した。青色光補光による草丈の減少と主茎節数の増加が観察されたが、これらの変化は同時に観察された青色光補光による花成阻害に起因すると考えられた。一方、収穫された葉におけるペリルアルデヒド含有量は青色光照射によって有意に増加した。 [Abstract] Two cultivars of perilla, 'Kishu-zairai' (Perilla frutescens var. crispa f. purpurea, red s mooth 1 eat) and 'Aka-chirimen-shiso' (Perilla frutescens var. crispa f. crispa, red wrinkled leaf), were grown in a greenhouse and were lighted continuously by blue LEDs (460nm, approximately 10μmol/m^2/s). As a result, it was revealed that the blue supplemental-lighting reduced the plant height and increased the number of nodes on main stem in both kind of perilla. These two phenomena were thought to be associated with the inhibition of flower formation by the blue supplemental-lighting. While, the content of perillaldehyde (a kind of functional chemical component) in harvested leaves was increased by the blue supplemental-lighting.
  • 2Bp10 Isolation and characterization of wound-induced compounds in Citrus hassaku leaves, Asai Tomonori, Matsukawa Tetsuya, Ishihara Atsushi, Kajiyama Shin'ichirou, 日本生物工学会大会講演要旨集, 63,   2011 08 25 , http://ci.nii.ac.jp/naid/110008911296
  • Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima., 大池 達矢, 松川 哲也, 岡南 政宏, 阿野 貴司, 梶山 慎一郎, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 27, 29, 37,   2011 03 01 , http://ci.nii.ac.jp/naid/120005735121
    Summary:[要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60%の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60%) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
  • Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima., 大池 達矢, 松川 哲也, 岡南 政宏, 阿野 貴司, 梶山 慎一郎, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 27, 29, 37,   2011 03 , http://ci.nii.ac.jp/naid/120003002881
    Summary:[要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60%の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60%) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
  • Quantitative analysis of phorbol esters in the wastes and intermediate products of Jatropha bio-diesel production., 松川 哲也, 鈴木 遥, 梶山 慎一郎, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 26, 13, 22,   2010 09 01 , http://ci.nii.ac.jp/naid/120005735109
    Summary:トウダイグサ科の熱帯性植物であるJatropha curcasは, 種子に重量比40-60%の中性脂質を含み, 半乾燥地や貧栄養土壌などの荒廃地でも生育可能であるため, 近年新たなバイオディーゼル燃料の原料植物として注目されている。しかし, 本植物の種子には発ガン促進物質であるphorbol esterを始めとする種々の毒性物質を含んでおり, その生産者の健康や環境への影響が懸念されている。そこで, 本研究では精油工程で産出される廃棄物や, 中間生成物に含まれるphorbol ester類の定量分析を行った。その結果, 搾油工程および脱ガム工程では中間生成物および廃棄物ともにphorbol ester類が多く含まれていた。一方, 脱酸工程, メチル化工程ではphorbol ester量が減少することが見いだされた。また, 最終生産物であるバイオディーゼル燃料からもphorbol ester類が微量検出された。本研究の結果は,J.curcas由来バイオディーゼルのリスク評価に寄与すると期待される。 (英文) Jatropha curcas, a tropical plant belonging to the family of Euphorbiaceae, has recently been attracted the considerable attention as a potential source for bio-diesel due to the high adaptability to semi-arid areas and the high oil content (40-60%) in seed kernel. The seed, however, contains several toxic compounds including phorbol ester, well known as a tumor promoting agent. Therefore, influence of these toxic compounds on environment and people engaged in the production should be concerned. In this study, the levels of phorbol esters in the wastes and intermediate products of different refining steps were quantitatively analyzed. Our results showed that both intermediate products and wastes obtained from pressing and degumming processes exhibited high phorbol esters contents, whereas levels of phorbol esters decreased during neutralization and transesterification. In addition, bio-diesel fluid, the final products of refining steps, also contains trace amount of phorbol esters. Our results may contribute to assess the risk of J. curcas bio-diesel.本研究は近畿大学生物理工学部戦略的研究No. 08-II-02の助成を受けた. , 本研究の一部は住友財団奨励金の助成を受けた. , Kajiyama, Shin'ichiro
  • 2Hp02 Quantitative analysis of phorbol esters in the wastes from Jatropha bio-diesel production, Suzuki Haruka, Matsukawa Tetsuya, Kajiyama Shin'ichiro, 日本生物工学会大会講演要旨集, 21,   2009 08 25 , http://ci.nii.ac.jp/naid/110007500611
  • Pollen morphology of Citrus species and cultivars maintained at germplasm conservation in Kinki University, 仁藤 伸昌, 松川 哲也, 伊東 卓爾, 我藤 雄, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 23, 21, 30,   2009 03 01 , http://ci.nii.ac.jp/naid/120005735087
    Summary:Departmental Bulletin Paper
  • Germination of Pollen Grain in Citrus Cultivars from Germplasm Conservation at Kinki University, 仁藤 伸昌, 松川 哲也, 伊東 卓爾, 我藤 雄, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 22, 19, 24,   2008 09 01 , http://ci.nii.ac.jp/naid/120005734924
    Summary:Departmental Bulletin Paper
  • Germination of Pollen Grain in Citrus Cultivars from Germplasm Conservation at Kinki University, 仁藤 伸昌, 松川 哲也, 伊東 卓爾, 我藤 雄, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 22, 19, 24,   2008 09 , http://ci.nii.ac.jp/naid/120001182002
    Summary:Departmental Bulletin Paper
  • C222 Subcellular localization and biosynthesis of hordatines in barley, Ishizuka Akihiro, Matsukawa Tetsuya, Ishihara Atsushi, Nishioka Takaaki, 講演要旨集, 33, 33,   2008 03 11 , http://ci.nii.ac.jp/naid/110006858225
  • Induction of chorismate mutase by an elicitor in oat leaves, MATSUKAWA Tetsuya, ISHIHARA Atsushi, IWAMURA Hajime, Chemical Regulation of Plants, 35, 2,   2000 12 25 , http://ci.nii.ac.jp/naid/110001855785
  • Involvement of peroxidase in metabolism of oat phytoalexin avenanthramides, OKAZAKI Yozo, MATSUKAWA Tetsuya, ISHIHARA Atsushi, IWAMURA Hajeme, Chemical Regulation of Plants, 35, 2,   2000 12 25 , http://ci.nii.ac.jp/naid/110001855786
  • Induction of chorismate mutase by an elicitor in oat leaves, Matsukawa Tetsuya, Ishihara Atsushi, Iwamura Hajime, 植物化学調節学会研究発表記録集, 35, 11, 12,   2000 11 02 , http://ci.nii.ac.jp/naid/110001853506
    Summary:Avenanthramides, a series of substituted N-cinnamoylanthranilate derivatives, are phytoalexins in oats. Hydroxycinnamate and anthranilate are derived from the shikimate pathway which branches at chorismate. Chorismate mutase (CM) is the first enzyme of the branch that leads to phenylalanine and phenylpropanoid biosynthesis. The induction of CM was investigated in primary oat leaves using oligo-N-acetylchitooligosaccharides as elicitors. The CM activity started to increase 6 hr after elictitation and reached a maximum by 9 hr. Among the oligo-N-acetylchitooligosaccharides tested, tetra-, penta-, and hexasaccharides effectively induced the enzyme activity in a dose-dependent manner. The activity was separated into two major peaks by anion exchange chromatography indicating that CM consists of at least two isoforms. Comparison of untreated and elicitor-treated leaves revealed that only one CM isoform was responsive to the elicitor. These results suggest a regulatory role of CM in avenanthramide biosynthesis.
  • Involvement of peroxidase in metabolism of oat phytoalexin avenanthramides, Okazaki Yozo, Matsukawa Tetsuya, Ishihara Atsushi, Iwamura Hajime, 植物化学調節学会研究発表記録集, 35, 13, 14,   2000 11 02 , http://ci.nii.ac.jp/naid/110001853507
    Summary:Avenanthramides, a series of substituted N-cinnamoylanthranilate derivatives, are phytoalexins in oats. The amounts of accumulated avenanthramides following elicitor treatment decreased after they reached a maximum. This accumulation was accompanied by deposition of fluorescent compounds in cell walls. In addition, the substrate specificity of the biosynthetic enzymes for avenanthramides was not necessarily correlated with composition of avenanthramides in elicitor solution. These findings suggested that avenanthramides are metabolized after their accumulation. To confirm this hypothesis, avenanthramides labeled with ^<13>C were exogenously applied into elicitor solutions. The amounts of exogenous avenanthramides decreased rapidly, and the rate of decrease was enhanced by elicitor treatment. This decreased of avenanthramides was partially inhibited by application of catalase into elicitor solution. In addition, we found that activity of peroxidase was induced by elicitor treatment in apoplastic fractions from oat leaves. These findings indicated that avenanthramides are metabolized by the action of peroxidases in oat leaves. Avenanthramides may thus be involved in the physical reinforcement of cell walls.
  • Induction of anthranilate biosynthesis by an elicitor in oat leaves., MATSUKAWA Tetsuya, ISHIHARA Atsushi, IWAMURA Hajime, Chemical Regulation of Plants, 34, 2,   1999 12 25 , http://ci.nii.ac.jp/naid/110001846942
  • 71 Induction of anthranilate biosynthesis by an elicitor in oat leaves, Matukawa Tetsuya, Ishihara Atushi, Iwamura Hajime, 植物化学調節学会研究発表記録集, 34,   1999 10 01 , http://ci.nii.ac.jp/naid/110001853481
    Summary:Avenanthramides, a series of substitute N-cinnamoylanthranilate derivatives, are phytoalexins in oats. Anthranilate, a precursor of avenanthramide, is synthesized from chorismate and glutamine by anthranilate synthase (AS). The induction of AS activity after elicitor treatment was investigated in primary oat leaves. The AS activity started to increase 3 hr after elicitation and reached a maximum by 6 hr. Among the oligo-N-acetylchitooligosaccharides tested, tetra-, penta-, and hexasaccharides effectively induced the enzyme activity in a dose-dependent menner. These results were well correlated with the accumulation of avenanthramides, suggesting a regulatory role of AS in avenanthramides biosynthesis.
  • C-29 Induction of tyramine feruloyltransferase (TFT) activity by wounding in maize., KAWATA Naoki, ISHIHARA Atsushi, MATSUKAWA Tetsuya, IWAMURA Hajime, 植物化学調節学会研究発表記録集, 33, 63, 64,   1998 10 01 , http://ci.nii.ac.jp/naid/110001853377
    Summary:Tyramine feruloyltransferase (TFT) catalyzes the condensation reaction to feruloyltyramine from tyramine and feruloyl-CoA. Wounding strongly induced this enzyme activity in maize leaves. TFT activity started to increase 3 hr after wounding and reached a maximum by 36 hr, being 11 times higher than the activity in control leaves. For characterization, TFT from wounded leaves was partially purified by (NH_4)_2SO_4 precipitation and two steps of anion exchage chromatography on DEAE-Sepharose and Mono Q. The best substrate was tyramine among tested amines, although phenethylamine, tryptamine and dopamine served as acyl acceptors. On the other hand, for acyl donar, feuloyl-CoA was most rapidly converted to a respective amide by TFT among hydroxycinnamoy-CoA esters.
  • 60 Induction of hydroxycinnamory-CoA:hydroxyanthranilate N-hydroxycinnamoyltransferase (HHT)activity in oat leaves by victorin C., Ishihara Atsushi, Matsukawa Tetsuya, Miyagawa Hisashi, Ueno Tamio, Mayama Shigeyuki, Iwamura Hajime, 植物化学調節学会研究発表記録集, 32,   1997 11 28 , http://ci.nii.ac.jp/naid/110001854051

Research Grants & Projects

  • Chemotaxonomy of citrus