KINDAI UNIVERSITY


*A space between the first name and last name, please enter

KATO Nobuhiro

Profile

FacultyDepartment of Biomedical Engineering / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/347-katou-nobuhiro.html
URL
Mail
Last Updated :2020/09/06

Education and Career

Academic & Professional Experience

  •   2017 04 ,  - 現在, Professor, Department of Biology-Oriented Science and Technology, Kindai University
  •   2006 04 ,  - 2017 03 , Associate Professor, Department of Biology-Oriented Science and Technology, Kindai University
  •   2006 08 ,  - 2007 07 , Visiting Scholar, Department of Engineering, University of Michigan
  •   2002 04 ,  - 2006 03 , Lecturer, Department of Biology-Oriented Science and Technology, Kindai University
  •   1998 04 ,  - 2002 03 , Faculty of Biology-Oriented Science and Technology, Kindai University

Research Activities

Research Areas

  • Life sciences, Medical systems
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Measurement engineering
  • Informatics, Mechanics and mechatronics
  • Nanotechnology/Materials, Nano/micro-systems
  • Nanotechnology/Materials, Nanomaterials
  • Life sciences, Biomaterials
  • Life sciences, Biomedical engineering

Research Interests

  • Microfluidics, Microneedles, photo-lithography, bio-MEMS, Atomic Force Microscope

Published Papers

  • Preparation of ultrathin opaque white hydroxyapatite sheets and their aesthetic effects, 本津 茂樹, 平井 瑞樹, 片山 一郎, 山本 衛, 加藤 暢宏, 吉川 一志, Journal of bio-integration, Journal of bio-integration, 7(1), 39 - 43, Dec. 2017
  • 牛体外受精技術の高度化による高齢雌牛からの産子生産, 谷口俊仁, 加藤暢宏, 佐伯和弘, 畜産技術, 畜産技術, (727), 22 - 25, Dec. 2015
  • P-72 Preparation and evaluation of potassium-containing hydroxyapatite sheet for dentin hyperesthesia treatment, Hatoko Y, Yamamoto E, Kato N, Nishikawa H, Yoshikawa K, Furuzono T, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices, The journal of the Japanese Society for Dental Materials and Devices, 34(5), 416 - 416, Sep. 2015
    Summary:In the therapy for hypersensitivity of tooth, it is known that potassium (K) compounds are often added in the toothpaste because it is believed that neurotransmission is blocked by potassium ions. Recently, we have been proposing the attachment therapy of a hydroxyapatite (HAp) sheet on dentin for hyperesthesia. It is thought that addition of K ions in HAp sheet is more effective for relieve pain of tooth hypersensitivity. In this study, we prepared K-HAp thin film and evaluated it. K-HAp sheet was prepared by using a process for preparing thin film. After that, we evaluated the interface between K-HAp sheet and dentin.
  • Direct Deposition of an Apatite Film on Dentin Using the Er:YAG Laser-Ablation Method, HONTSU Shigeki, YAMAMOTO Ei, KATO Nobuhiro, YOSHIKAWA Kazushi, YASUO Kenzo, YAMAMOTO Kazuyo, Journal of Japanese Society for Laser Dentistry, Journal of Japanese Society for Laser Dentistry, 26(1), 10 - 16, Apr. 2015
    Summary:Ideal restoration of tooth substances requires hydroxyapatite (HAp), of which the tooth is composed. However, restoration treatment with HAp is not yet possible, as adherence to ceramics is very difficult. In the present study, we examined a tooth restoration technique using the intraoral laser ablation method. A thin layer of α-tricalcium phosphate (α-TCP) was deposited on the dentin surface by the ablation phenomenon using an Er:YAG laser, which has already been introduced into dental treatment, to irradiate the target α-TCP. The deposited layer was then hydrolyzed by dripping pure water on its surface in order to create a hydroxyapatite (HAp) coating. In this study, we developed a compact pulsed laser deposition (PLD) system using the Er:YAG laser. The interface structure between the HAp coating and the dentin surface was observed by scanning electron microscope (SEM). Electron micrographs showed that the HAp layer was formed on the dentin surface. Moreover, dentinal tubules were sealed with HAp particles. We evaluated the blockade effect of dentinal tubules using Pashle's method. The measured average value for the sealing rate of the dentinal tubules was 85.6±8.6%, which was greater than the value with resin coating treatment. The adhesive strength of HAp films deposited on dentin was evaluated by quasi-static tensile tests. On mechanical evaluation, the adhesive strength was greater than 3.8 MPa. The present results suggest that this technique will be useful for the repair of dentin and the treatment of hyperaesthesia.
  • Fabrication of a HA films using the Er:YAG laser, Journal of bio-integration, Journal of bio-integration, 4(1), 65 - 69, Jul. 2014
  • Restration of Tooth Enamel Using a Flexible Hydroxyapatite Sheet Coated with Tricalsium Phoshate, Yamamoto, E. Kato, N Isai, A. Nishikawa, H. Kusunoki, M. Yoshikawa, K. Hontsu, S, Bioceramics Development and Applications, Bioceramics Development and Applications, S1, 006, Aug. 2013 , Refereed
  • Ultrathin Amorphous Calcium Phosphate Freestanding Sheet for Dentin Tublue Sealing, Kato, N Yamamoto, E. Isai, A. Nishikawa, H. Kusunoki, M. Yoshikawa, K. Hontsu, S, Bioceramics Development and Applications, Bioceramics Development and Applications, S1, 007, Aug. 2013 , Refereed
  • Examination of enamel repairing method using ultrathin apatite sheet (Part2), HONTSU S, YOSHIKAWA K, ISAI A, YAMAMOTO E, KATO N, NISHIKAWA H, KUSUNOKI M, HASHIMOTO Y, YAMAMOTO K, The journal of the Japanese Society for Dental Materials and Devices, The journal of the Japanese Society for Dental Materials and Devices, 32(2), 125 - 125, Mar. 2013
    Summary:We have successfully created a flexible hydroxyapatite (HAp) sheet of less than a few micrometers, and we have been trying to establish the all-HAp restoration technique for tooth enamel. In this study, we evaluated the re-calcification layer at the various sheets (amorphous HAp, HAp, amorphous HAp/amorphous calcium phosphate: ACP, HAp/ACP)-enamel interface by scanning electron microscope (SEM).
  • Formation of the artificial enamel on dentin using the ultrathin apatite sheet, HONTSU S, YOSHIKAWA K, ISAI A, YAMAMOTO E, KATO N, NISHIKAWA H, KUSUNOKI M, HASHIMOTO Y, YAMAMOTO K, The journal of the Japanese Society for Dental Materials and Devices, The journal of the Japanese Society for Dental Materials and Devices, 31(5), 445 - 445, Sep. 2012
    Summary:We have fabricated a flexible hydroxyapatite (HAp) using a pulsed laser deposition (PLD) method and a film isolation technique. We adhered the HAp sheet to the dentine surface using a calcium phosphate aqueous in order to apply this sheet to hyperesthetic treatment. In the present study, the time dependence of the composition at the interfaces between the HAp/TCP sheet and the dentine was evaluated using X-ray diffraction measurement, and the adhesive strength between the HAp/TCP sheet and the dentine surface was evaluated by quasi-static tensile tests.
  • Examination of enamel repairing method using ultrathin apatite sheet (Part1), HONTSU S, YOSHIKAWA K, MATSUDA T, KATO N, NISHIKAWA H, KUSUNOKI M, HASHIMOTO Y, YAMAMOTO K, The journal of the Japanese Society for Dental Materials and Devices, The journal of the Japanese Society for Dental Materials and Devices, 31(2), 112 - 112, Mar. 2012
    Summary:We have succeeded to fabricate flexible hydroxyapatite (HAp) sheet for adhering directly onto tooth and bone surfaces using a pulsed laser deposition method and a film isolation technique. We have investigated a method to regenerate the tooth enamel by just attaching the flexible HAp sheet onto the tooth surface with artificial saliva. In this paper, we report the result of adhering the HAp sheet to the enamel assisted by an TCP over-deposition and calcium phosphate aqueous solution in order to reduce the period required to regenerate the enamel.
  • Adsorption of Type I Collagen onto Poly (dimethylsiloxane) Microfluidic Device and Time-lapse Observation of HL60 Cells under Continuous Perfusion., 森本 康一, 西山 裕之, 國井 沙織, 角田 昌明, 加藤 暢宏, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, (26), 39 - 46, Sep. 01 2010
    Summary:本研究は近畿大学生物理工学部戦略的研究No.06-II-1, 2006およびNo.07-IV-3, 2007の助成を受けた,灌流培養環境下でHL60細胞とI型コラーゲンとの相互作用を観察するため, poly (dimethylsiloxane)を用いて微小流路デバイス(幅700μm×高さ100μm×長さ20mm)を作製した. 矩形断面を有する微小流路内壁4面すべてを酸素プラズマ処理し, ニワトリI型コラーゲンとウシ胎児血清成分タンパク質を灌流して固定化した. 固定化されたタンパク質の吸着量をSDS-ポリアクリルアミドゲル電気泳動にて定量的に調べた結果, 血清タンパク質に対して酸素プラズマ処理の効果は認められなかったが, I型コラーゲンでは酸素プラズマ処理の効果が経時的に減少するに従い, その固定化量が増加した. 作製した微小流路デバイスを用いた0.2μL/minの灌流培養下で, HL60細胞はI型コラーゲンにのみローリングあるいは接着挙動を示した. 酸素プラズマ処理の効果はI型コラーゲンに対する細胞接着に大きく影響した. HL60細胞の接着挙動は固定化されたI型コラーゲン量に単純に比例せず, その構造変化を認識することが示唆された. (英文) We developed microfluidic device (W 700μm×H 100 gμm×L 20 mm) with poly (dimethylsiloxane) to observe adhesion of HL60 cells on type I collagen under continuous perfusion. The surface of poly (dimethylsiloxane) was treated by oxygen plasma and then coated by fetal calf serum proteins or chicken type I collagen. By using SDS-polyacrylamide gel electrophoresis, we showed that treatment of oxygen plasma had no effect on binding of serum proteins on the polymer surface. However, the amount of collagen on the surface increased by decreasing of the effect of plasma treatment. On the serum protein-coated polymer surface, HL60 cells fully flowed through under 0.2μL/min flow rate. On the other hand, HL60 cells bound to the collagen-coated polymer surface and subsequently adhered to the collagen-coated polymer surface. We suggest that the conformation of collagen would alter depending on the degree of oxygen plasma treatment of PDMS and HL60 cells would recognize the difference in collagen structure.
  • Cooperation between an Enterprise and a School in Mechanical Design Education by using 3D-CAD, FUJII Masao, KATO Nobuhiro, SHIMIZU Takaki, SATOMI Kenichi, TAGUCHI Shinya, Transactions of the Japan Society of Mechanical Engineers C, Transactions of the Japan Society of Mechanical Engineers C, 73(725), 30 - 35, Jan. 25 2007
    Summary:3D-CAD system has been used in practical product design to realize simultaneous engineering and also to improve productivity. Although 3D-CAD is a widely used and highly effective tool in mechanical design, it also has its drawbacks: mastery of 3D-CAD skills is rather complex and time-consuming. In an attempt to simplify the learning process and cut down on learning time, we have developed training programs for mechanical design such as a concept making, a family tree and a team design method, and e-Learning contents for the 3D-CAD operation skills. The effectiveness of these training program and e-Learning contents has been confirmed by cooperative activity between an enterprise and a school in mechanical design education.
  • 原子間力顕微鏡によるウシ正常および先体反応精子の微細構造の検討(Fine Surface Structure of Bovine Acrosome-Intact and Reacted Spermatozoa Observed by Atomic Force Microscopy), 佐伯 和弘, 住友 範生, 永田 由紀, 加藤 暢宏, 細井 美彦, 松本 和也, 入谷 明, The Journal of Reproduction and Development, The Journal of Reproduction and Development, 51(2), 293 - 298, Apr. 2005
    Summary:The atomic force microscope (AFM) provides nanometer resolution, topographic data of the natural surface structure of materials. We studied the topology of the surface structure of bovine sperm heads during the acrosome reaction by AFM. In addition, we numerically analyzed the areas of the median sagittal plane of the sperm heads. Bovine frozen-thawed spermatozoa were washed, capacitated by heparin, and incubated with lysophosphatidylcholine (LPC) to induce the acrosome reaction, smeared on a cover glass, air-dried, and observed with AFM using the dynamic force (tapping) mode. AFM analysis of spermatozoa showed the clear surface structure of acrosomes, equatorial segments, postacrosomal regions and necks. Although AFM images of spermatozoa capacitated by heparin had complete acrosomes, most spermatozoa treated with LPC had no acrosomal caps as shown by AFM. These observations coincided with those obtained by light microscopy after staining with naphthol yellow S and erythrosin B. Furthermore, numerical analysis of AFM images indicated that areas of the median sagittal plane of the anterior portions of acrosome-reacted sperm heads (2679 ± 616 pixels) were approximately 40% less than those of intact heads (4535 ± 174 pixels, P<0.05). These results indicate that AFM can usefully observe and numerically analyze the fine surface structures of bovine spermatozoa.
  • Computer Generated Hologram on Curved Surface for High Power CO2 Laser Beam Shaping, (18), 87 - 91, Nov. 2004 , Refereed
  • Fabrication of Multilevel Copper Computer Generated Hologram for High Power CO_2 Laser Beam Shaping, HAGINO Hideki, PARK Choong Sik, YOKOI Masayuki, KATO Nobuhiro, MITSUMATA Mari, KIKUTA Hisao, IWATA Koichi, Journal of the Japan Society for Precision Engineering, Contributed Papers, Journal of the Japan Society for Precision Engineering, Contributed Papers, 70(9), 1180 - 1185, Sep. 05 2004
    Summary:A multilevel computer generated hologram (CGH) was fabricated for shaping a laser beam profile of a high-power CO2 laser. The surface relief has eight height levels with 0.94μm in step heights. A copper surface relief was formed on a flat copper substrate by electroplating with photo-resist masks. Standard deviation of the surface step height was less than 22% of the designed height. The CGH changes a CO2 laser beam of 14mm in diameter to a rectangular beam of 1mm×4mm and its diffraction efficiency was 69.2%. A 200W CO2 laser beam of 14mm in diameter did not damage the CGH surface.
  • Cooperation between an Enterprise and a School in Mechanical Design Education by using 3D - CAD, FUJII M., KATO Nobuhiro, SHIMIZU Takaki, SATOMI Kenichi, Memories of The School of B. O. S. T. of Kinki University, Memories of The School of B. O. S. T. of Kinki University, 14(14), 101 - 108, Sep. 01 2004
    Summary:The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. Particularly, a three dimensional CAD(3D-CAD) system has been used in practical product design to realize simultaneously engineering and also to improve the productivity. At an enterprise, mechanical engineers have been required to master 3D-CAD as soon as possible. On the other hand, many students graduating from a school will be engaged in the enterprise so that they should study 3D-CAD. Therefore we have been cooperating for 3D-CAD education and confirmed its effectiveness.
  • Mechanical Evaluation of the Human Femur by Holographic Interferometry, Matsumoto Toshiro, Kojima Arata, Izuchi Noboru, Kato Nobuhiro, Watanabe Toshiaki, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, (13), 73 - 78, Feb. 01 2004
    Summary:The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. Double exposures and real-time holographic interferometry were used to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to simulate properly the situation of a femur under physiological muscles, the 3D finite element method (FEM) was used for our analyses. After modeling the femur, which consisted of the meshes as eight noded elements for each section of diaphysis, stress and strain were calculated by means of the 3D FEM. The following results were obtained : (1) the deformations increased with the progress of osteoporosis, (2) femur with osteoporosis showed obvious rotational components around the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • An Example of Product Design Education by using 3D-CAD in the University of Kinki, FUJII Masao, KATO Nobuhiro, Journal of Japan Society for Design Engineering, Journal of Japan Society for Design Engineering, 38(8), 377 - 382, Aug. 05 2003
  • Computer generated hologram on a curved surface for CO2 laser beam shaping, Hideki Hagino, Choong Sik Park, Nobuhiro Kato, Mari Mitsumata, Hisao Kikuta, Koichi Iwata, Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering, Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering, 69(3), 417 - 421, Mar. 2003 , Refereed
    Summary:This paper describes a computer generated hologram (CGH) on a curved surface for laser machining. The CGH fabricated on a parabolic copper mirror converts a 14mm diameter Gaussian beam of a CO2 laser into a shaped beam whose intensity distribution is uniform (4mm) in x direction and gaussian (1mm) in y direction. The binary CGH pattern was drawn by a laser direct writing machine with a focus servo mechanism. To obtain an accurate step height of CGH pattern, a palladium thin film was placed between the copper mirror and a plated copper layer 3.75μm thick. While the plated layer was etched by a ferric chloride solution, the palladium film prevented the substrate mirror from being etched. The beam shaping by the fabricated CGH was demonstrated experimentally with a low power CO2 laser and an infrared-camera.
  • Computer generated hologram on a curved surface for CO2 laser beam shaping | Computer generated hologram on a curved surface for CO2 laser beam shaping, Hagino H, Park C.S, Kato N, Mitsumata M, Kikuta H, Iwata K, Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering, Seimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering, 69(3), 417 - 421, 2003 , Refereed
  • Education of Product design at multimedia age, FUJII M., Kato Nobuhiro, Memories of The School of B. O. S. T. of Kinki University, Memories of The School of B. O. S. T. of Kinki University, 11(11), 136 - 155, Dec. 01 2002
    Summary:The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. This paper describes the progress of mechanical design technology and the importance of the education by using 3dimensional computer aided design (3DCAD) system at multimedia age. In the product design, it is important to promote the creative and intelligent design operation and also to construct the effective educational curriculum by using 3DCAD.
  • Reduction in Feedback Bandwidth of the Force-Controlled Atomic Force Microscope Using a Polyimide Cantilever, KATO Nobuhiro, MATSUMOTO Toshiro, KIKUTA Hisao, NAKAMURA Yasuhiro, IWATA Koichi, Japanese Journal of Applied Physics, Japanese Journal of Applied Physics, 40(11), 6594 - 6599, Nov. 15 2001
    Summary:In this paper we present a method of reducing the bandwidth required in the force-controlled atomic force microscope (AFM) for measuring the force curves. System stability strongly depends on the quality factor of vibration (Q) of the cantilever. As polyimide has a low Q, a cantilever fabricated from it reduces the bandwidth required in the feedback system. A polyimide cantilever with dimensions of 540 × 155 × 3.4 μm3 is fabricated to evaluate its Q in vacuum, density and Young's modulus. The measured Q is 50, which is much smaller than those of conventional cantilevers used for AFM. Using the measured mechanical properties of the polyimide, the required frequency response of the feedback system is estimated. Typical force curve measurements are confirmed by some numerical simulations.
  • System analysis of the force-feedback method for force curve measurements, Nobuhiro Kato, Hisao Kikuta, Takashi Nakano, Toshiro Matsumoto, Koichi Iwata, Review of Scientific Instruments, Review of Scientific Instruments, 70, 2402 - 2407, Dec. 1999 , Refereed
    Summary:The force-feedback method is a promising technique to measure accurate force curves in atomic force spectroscopy. In this article, we describe criteria of the stability for the force-feedback system containing a proportional amplifier with first-order lag. We derive the criteria from an equation of the cantilever movement with higher-order vibration modes under force interaction. The criteria predict that a proportional amplifier with a certain gain and a "very high" cutoff frequency is required to stabilize the feedback system when the critical force gradient exceeds the cantilever stiffness. To measure a force curve, including the steep part (-17 N/m) with a soft (0.2 N/m) and poor-damping (2 X 10-7 kg/s) cantilever, the amplifier for the force-feedback controller must have a frequency response wider than 16 MHz and gain larger than 16.8. Moreover, the feedback controller with only an integrator, which is popular in scanning tunneling microscopy and atomic force microscopy, does not contribute to reducing the discontinuity of the force curve. © 1999 American Institute of Physics.
  • Morphological changes of bovine sperm surfaces during sperm capacitation and acrosome reaction by atomic force microscopy, 佐伯 和弘, 加藤 暢宏, 細井 美彦, 松本 和也, 加藤 博己, 入谷 明, Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University, Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University, (2), 26 - 32, Mar. 1999
    Summary:継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University走査型近視野原子間力顕微鏡は、観察したい材料に処理を加えずに、そのままの表面形状を nanometer単位で非常に微細に観察でき、容易にその3次元構築像が得られる。今回この顕微鏡を用いて凍結保存されたウシ精子を融解後、heparinによる受精能獲得処理さらにlysophosphatidylcholine (LC) による先体反応誘起処理を行い、その頭部の表面形状を観察した。融解直後、Percoll洗浄後およびheparin処理後の精子は先体帽、先体赤道部、後核帽および頚部が観察された。洗浄処理や heparin処理により精子表面の形状には変化が見られなかった。LC処理後の精子は、先体が観察できず頭部先端部分に胞状化した先体の一部あるいは先体顆粒の結晶と思われる粒子が数多く付着している像が観察され、LC処理により先体反応が誘起されたと思われた。また、いずれのサンプルでも、多くの結晶構造が精子付近あるいは精子表面上に観察された。これは、精子を培養液とともに直接スライドグラス上に塗沫・風乾したため塩類の結晶が形成されたためと推察された。 (英文) Atomic force microscope (AFM) provides nanometer-resolved, topographic data images of the natural surface structure of the objects. AFM also has a novel 3 D image-contrast mechanisms. In this study, surfaces of bovine sperm heads frozen-thawed, Percoll-washed, capacitated and acrosome-reacted were observed with the AFM. Spermatozoa were smeared on glass coverslip and air-dried. AFM images of spermatozoa after thawing and washing clearly showed their acrosomal caps, equatorial segments, postoacrosomal regions and necks. Images of capacitated spermatozoa treated with heparin also showed their complete acrosomal caps. However, acrosome-reacted spermatozoa induced by lysophosphatidylcholine were lack of their acrosome caps. Images also indicated salt crystals which were formed during airdrying smears of sperm suspension.
  • Resonance Characteristics of Micro Cantilever in Liquid, HIRAI Yoshihiko, MORI Ryosuke, KIKUTA Hisao, KATO Nobuhiro, INOUE Koji, TANAKA Yoshio, Japanese Journal of Applied Physics, Japanese Journal of Applied Physics, 37(12), 7064 - 7069, Dec. 01 1998
    Summary:The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also, the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results.
  • Formation of Hydroxyapatite Thin Films on Surface-Modified Polytetrafluoroethylene Substrates, HONTSU Shigeki, NAKAMORI Masaya, KATO Nobuhiro, TABATA Hitoshi, ISHII Junya, MATSUMOTO Toshirou, KAWAI Tomoji, Japanese Journal of Applied Physics Pt. 2 Letters, Japanese Journal of Applied Physics Pt. 2 Letters, 37(10), L1169 - L1171, Oct. 01 1998
    Summary:An ArF excimer laser deposition technique is used to fabricate hydroxyapatite [Ca-10(PO4)(6)(OH)(2)]-(HAp) thin films on surface-modified polytetrafluoroethylene (PTFE) substrates. The surface of PTFE is modified by a sodium-naphthalene complex in a glycol ether solvent. X-ray diffraction (XRD) studies indicate that as-deposited films prepared at substrate temperatures below the glass transition temperature of PTFE (327 degrees C) are in an amorphous state. The crystallization of as-deposited films needed annealing for 10 h at 310 degrees C. The structure, surface morphology and bond strength of HAp films were evaluated using XRD, the atomic force microscopy (AFM) and the Instron testing machine. The tensile bond strength of HAp films on surface-modified PTFE was 6.0 MPa, which is one order of magnitude larger than that of films on non-surface-modified PTFE, demonstrating its potential for practical applications.
  • On the Polymer Thickener for Viscosity-Stabilized Solar Pond, MATSUMOTO Toshiro, KATO Nobuhiro, TAGA Masao, Japanese journal of applied physics Pt. 1 Regular papers, brief communications & review papers, Japanese journal of applied physics Pt. 1 Regular papers, brief communications & review papers, 37(9), 5088 - 5093, Sep. 01 1998
    Summary:The optimal physical properties of an ionized polyacrylamide solution, selected as the most suitable thickener for the insulating layer of a viscosity-stabilized solar pond, are described. Light transmittance, viscosity and viscosity degradation were obtained for a range of thicknesses from 1 cm to 15 cm (1-15 cm): weight concentrations of 0.1-0.5% (0.1-0.5 wt%) and temperatures of the polymer of 20-80 degrees C. Onset of convection was also measured for a given thickness of the polymer solution. An exposure test was carried out to prevent degradation of the polymer using an antioxidant. It was confirmed that the viscosity was not decreased and, on the contrary, increased slightly after 100 days. It was shown experimentally that the critical Rayleigh number coincided with the theoretical values reported by Tien rr of. [Int. J. Heat Mass Transf. 12 (1969) 1173]. Transmittances of the polymeric gel for the case of vessels greater than 5 cm in thicknesses were better than those of the SPR402 solution (0.5 wt%).
  • A New Method for Strain Measurement by Using Multiple Color Filtering, Matsumoto Toshiro, Nagae Sadahiko, Kato Nobuhiro, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 3(3), 38 - 44, Jun. 01 1998
    Summary:A semi-quantitative measurement of the strain distribution by two-dimensional color dispaly is presented. The strain components are converted into color band intensity distributions in the image of the specimen. The method is based on a optical technique with the aid of the spatial filtering theory. To show a usefulness of the method, it was carried out to measure the strain distribution of a plane plate with a round hole.
  • Deformation measurement of lumbar vertebra by using holographic interferometry, Toshiro Matsumoto Nobuhiro, Kato Kentaro Tamamura Arata, Kojima Noboru Izuchi, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 2, 50 - 58, 1997 , Refereed
  • Stability of ultrathin amorphous calcium phosphate artificial enamel sheets under simulated intraoral environment condition, Nobuhiro Kato, Mizuki Hirai, Ei Yamamoto, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 829 KEM, 16 - 20, Dec. 2019 , Refereed
    Summary:© 2020 Trans Tech Publications Ltd, Switzerland. Dental materials purely composed of calcium phosphate are ideal for dental medicine. We have been developing ultrathin amorphous calcium phosphate (ACP) sheets for enamel repair. In this study, long term stability of the ultrathin ACP sheets applied on enamel surfaces which kept in a simulated intraoral condition for 21 days was evaluated. Surface morphologies of the ACP sheets were observed by optical images. A boundary condition between the ACP sheet and the enamel was evaluated by cross-sectional SEM image. Durability of the ACP sheet was evaluated by a brushing test conducted after 21 days incubation. As a result of these evaluations, it was confirmed that the ACP sheets were stable even after long-term incubation under the simulated intraoral environment condition.
  • In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure, Daisaku Iwamoto, Nobuhiro Kato, Shunji Taniguchi, Yoshitomo Taguchi, Masao Kishi, Kazuhiro Saeki, International Journal of Biomaterials, International Journal of Biomaterials, 2018, 2018 , Refereed
    Summary:© 2018 Daisaku Iwamoto et al. Single embryo culture is useful for assessing the developmental competence of an embryo in detail. Recently, a device made of poly(dimethylsiloxane) (PDMS), which is biocompatible and nontoxic, has been widely used for culture various types of cells. However, PDMS plates are porous, causing the serious osmolality increment of the medium (over 600 mOsm/kg from Day 4 to Day 7). Here, we report that curing the PDMS under low pressure (LP-PDMS) greatly reduced the porosity, resulting in a constant osmolality of the medium. The blastocyst rate of single bovine embryos cultured with LP-PDMS microwell (MW) plates was the same as that of group-cultured embryos (25 embryos/50 μl droplet; control, P>0.05). These results indicate that MWs on a plate made of PDMS cured under low pressure can be successfully used for individual embryo culture.
  • Development of ultra-Thin opaque white hydroxyapatite sheet for restoration of enamel and aesthetic treatments of teeth, Nobuhiro Kato, Yuki Ido, Ei Yamamoto, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 758 KEM, 162 - 165, 2017 , Refereed
    Summary:© 2017 Trans Tech Publications, Switzerland. In cosmetic dentistry, bleaching and laminate veneer methods are common treatments to improve the appearance of teeth. However, there are limitations to these treatments, as they damage the enamel which is not capable of recovering naturally. In this study, novel hydroxyapatite (HAp) sheets, which enable both enamel restoration and aesthetic treatments, were developed. Results indicated that the lower range of the sintering temperatures, of the pulsed laser deposition targets, produced lighter shades of amorphous calcium phosphate (ACP) sheets. Subsequently, the post-Annealing process of the sheets induced crystallization processes of the ACP sheets, to form HAp sheets, and improved the shade rank of the sheet. The shade rank of the developed sheet (6.8 ± 0.2) was almost comparable to the average shade rank of the teeth of Japanese people, within a range of 6.5 to 7.0.
  • Adhesive evaluation by brushing tests for hydroxyapatite films fabricated on dentins using a water mist assisted Er:YAG laser deposition method, Ei Yamamoto, Nobuhiro Kato, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 758 KEM, 97 - 104, 2017 , Refereed
    Summary:© 2017 Trans Tech Publications, Switzerland. Hydroxyapatite (HAp) is one of the main structural components in tooth. Therefore, the HAp is an ideal material to repair tooth substances. However, it has not yet been realized that tooth enamel and dentin are artificially repaired using the HAp in operative dentistry. We have proposed and developed a tooth restoration technique with intraoral laser ablation using an Er:YAG (Erbium: Yttrium-Aluminum-Garnet) laser in order to fabricate HAp films on tooth surfaces in the atmosphere at room temperature. Although HAp thin films were formed on dentin surfaces using the Er:YAG laser deposition method, it has still been unclear how is the mechanical durability of the HAp film on tooth substances. In the present study, we conducted brushing tests to evaluate the durability of adhesion between the HAp film and dentin. To form HAp layers on dentin surfaces, an ablation phenomenon was induced by the Er:YAG laser irradiated to the target of α-Tricalcium phosphate (α-TCP) under humid conditions. The deposited layers were hydrolyzed by dripping artificial saliva on the surface at 37 degrees Celsius for 24, 48, 72, 120, and 168 hours to create HAp films. The films hydrolyzed for more than 48 hours were attached on the dentin surface after the brushing tests. This result indicates that the α-TCP layer deposited on the dentin surface changes into a crystallized HAp material by the hydrolyzation for more than 48 hours. The crystallization of the film possibly contributes to the high adhesive durability of the HAp film deposited on the dentin.
  • Preparation and application of a potassium-substituted calcium phosphate sheet as a dental material for treating dentin hypersensitivity, Nobuhiro Kato, Yuka Hatoko, Ei Yamamoto, Tsutomu Furuzono, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 720, 102 - 107, 2017 , Refereed
    Summary:© 2017 Trans Tech Publications. Potassium-substituted calcium phosphate free standing sheet for the treatment of dentin hypersensitivity was prepared and evaluated. The appropriate annealing condition for the target of pulsed laser deposition to prepare the potassium-substituted amorphous calcium phosphate (PS-ACP) sheet was investigated. The target annealed at 1000 °C was used to deposit the PS-ACP sheet. The annealing condition for the potassium-substituted hydroxyapatite (PS-HAp) sheet was evaluated by XRD and EDX. The potassium release rate from PS-ACP sheet in PBS, evaluated by ICP-MS, was 2.5 times larger than that of PS-HAp sheet. The robustness of the PS-ACP sheet was proved by the brushing test of the PS-ACP sheet affixed on a bovine dentin. The dentin tubules on the dentin surface was occluded by the PS-ACP sheet and which confirmed by SEM observations.
  • Optimization of humid conditions using an ultrasonic nebulizer for the fabrication of hydroxyapatite film with the Er:YAG laser deposition method, Ei Yamamoto, Nobuhiro Kato, Yuka Hatoko, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 720, 269 - 274, 2017 , Refereed
    Summary:© 2017 Trans Tech Publications. Restoration and maintenance of tooth substance are primary concerns in operative dentistry. Intact tooth is mainly composed of hydroxyapatite (HAp). However, there are no practical HAp materials for the treatment of tooth decay and hypersensitivity. We have newly proposed and developed a tooth restoration technique with intraoral laser ablation using an Er:YAG laser in order to form the HAp thin film on tooth surface in the atmosphere at room temperature. However, the fabricated HAp film is not uniform for the present due to the unsettled water condition at the material surface during the laser ablation. In the present study, we tried to optimize the humid condition using a nebulizer for the fabrication of HAp layer with the Er:YAG laser deposition method. The HAp layers were fabricated on a titanium substrate while changing the flow rate of water mist in order to determine the relationship between the spraying amount and the HAp layer structure. It is revealed that there exists the optimal humid condition to fabricate the uniform hydroxyapatite coating film with the Er:YAG laser deposition method.
  • Development of a Rapid Prototyping System for Microneedles Using Moving-mask Lithography with Backside Exposure, Takahisa Kai, Shunta Mori, Nobuhiro Kato, ADVANCED BIOMEDICAL ENGINEERING, ADVANCED BIOMEDICAL ENGINEERING, 5(5), 63 - 67, 2016 , Refereed
    Summary:Moving-mask lithography with backside exposure was utilized to generate master male mold for biodegradable polymer microneedle production. The microneedle shape was calculated from the exposure dose, mask geometry, and moving trajectory using a newly developed computer simulation. Two conditions (90 mu m aperture with 80 mu m diameter of circular movement, and 90 mu m aperture with 90 mu m diameter of circular movement) were selected to evaluate the moving-mask exposure effectiveness. By changing the moving trajectory, two different sizes of microneedles were obtained from a single-size aperture mask. The fabricated microneedle and calculated microneedle geometry showed good qualitative agreement. The geometrical difference was 2% in basal diameter and 8%-16% in height. Using the master male mold, biodegradable polymer microneedles made of chondroitin sulfate C sodium salt (CSC) were fabricated by casting from a poly-dimethylsiloxane female mold. The shape of the biodegradable CSC microneedles showed good agreement with the master male mold.
  • Development and Reliability Evaluation of Embryo Monitoring System using Time-Lapse Cinematography, Shizuka Miyata, Choong Sik Park, Yusuke Sando, Yuta Fujiki, Kazuhiro Saeki, Nobuhiro Kato, ADVANCED BIOMEDICAL ENGINEERING, ADVANCED BIOMEDICAL ENGINEERING, 5(5), 83 - 87, 2016 , Refereed
    Summary:A simple time-lapse cinematography (TLC) device was developed to enable detection, at the cleavage stages, of embryos that have the potential to develop into high-scoring blastocysts. The TLC device, which consists of an optical microscope system, an illumination system, a PC-controlled camera, and a focus adjustment unit, is encapsulated in a waterproof container to allow operation in a gas-controlled water jacket incubator. Operation of the device in a high humidity environment was ensured by an environmental test in an incubator. By capturing the images of bovine embryos every 10 min for several days, their growing process was precisely recorded. The feature quantities calculated from the temporal difference images were utilized to predict the cleavage timings of the embryos.
  • Overlaid ultrathin amorphous calcium phosphate sheet improves dentinal permeability inhibition rate, Nobuhiro Kato, Yuki Ido, Ei Yamamoto, Kenzo Yasuo, Kazushi Yoshikawa, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 696, 99 - 102, 2016 , Refereed
    Summary:© 2016 Trans Tech Publications, Switzerland. Dentin hypersensitivity is induced by mechanical stimuli or heat stimuli applied via exposed dentin tubules. Resin coating and laser-irradiation are used for treatment but have some problems such as poor biocompatibility or difficulty for laser power setting. We have been developing a treatment that creates artificial tooth enamel by attaching flexible ultrathin calcium phosphate sheet to seal the dentin tubule orifice. In this study, the quantitative evaluation of dentinal permeability inhibition rate (DPIR) improved by attaching ultrathin amorphous calcium phosphate (APC) sheets on human dentin in vitro is presented. The obtained DPIR by overlaid APC sheets application was 81.1 ± 5.7 %.
  • Adhesion properties of an apatite film deposited on dentine using Er:YAG Laser ablation method, Ei Yamamoto, Nobuhiro Kato, Kazushi Yoshikawa, Kenzo Yasuo, Kazuyo Yamamoto, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 696, 69 - 73, 2016 , Refereed
    Summary:© 2016 Trans Tech Publications, Switzerland. Tooth enamel cannot be naturally reconstructed once it is destroyed immoderately. Therefore, the restoration of enamel is important in dental treatment. Hydroxyapatite (HAp) can potentially be used for a novel dental biomaterial to repair the enamel. We have proposed a tooth restoration technique with intraoral laser ablation method. A thin layer of HAp was deposited on dentin surface in the atmosphere by ablation phenomenon using an Er:YAG (Erbium: Yttrium-Aluminum-Garnet) laser irradiated to the target of α-Tricalcium phosphate (α-TCP). Then, the deposited layer was hydrolyzed by dripping pure water on its surface in order to create HAp film. The adhesive strength between the HAp film and dentin was evaluated by quasi-static tensile tests. As a result of the mechanical evaluation, the adhesive strength was greater than approximately 3.8 MPa. It is clear that the HAp film created by the Er:YAG laser ablation method is attached tightly to the dentin.
  • Evaluation of dentin tubule sealing rate improved by attaching ultrathin amorphous calcium phosphate sheet, Nobuhiro Kato, Arata Isai, Ei Yamamoto, Hiroaki Nishikawa, Masanobu Kusunoki, Kazushi Yoshikawa, Kenzo Yasuo, Kazuyo Yamamoto, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 631, 258 - 261, 2015 , Refereed
    Summary:© (2015) Trans Tech Publications, Switzerland. Dentin hypersensitivity is induced by mechanical stimuli or heat stimuli applied via dentin tubules exposed by lost of dental enamel or gingival recession. Common treatments for dentin hypersensitivity are resin coating or laser irradiation. However, these treatments have some problems such as poor biocompatibility or insufficient durability. We have been developing a treatment that creates artificial tooth enamel by attaching flexible ultrathin calcium phosphate sheet having a crystal structure similar to that of tooth enamel to seal the dentin tubules. In this study, the quantitative evaluation of dentin tubule sealing rate improved by attaching ultrathin amorphous calcium phosphate (ACP) sheet on human dentin is presented. The obtained sealing-rate by APC sheet application was 70.9 ±4.8%.
  • A novel treatment for dentine cavities with intraoral laser ablation method using an Er:YAG laser, Ei Yamamoto, Nobuhiro Kato, Arata Isai, Hiroaki Nishikawa, Yoshiya Hashimoto, Kazushi Yoshikawa, Shigeki Hontsu, Key Engineering Materials, Key Engineering Materials, 631, 262 - 266, 2015 , Refereed
    Summary:© (2015) Trans Tech Publications, Switzerland. It has been well known that bone has piezoelectric properties and these properties have been considered to be caused by the shift of the center of symmetry of the positive and negative electrical charge due to the strain of the collagen fibers included in the bone. Thus, it has long been considered that there were no piezoelectric effects in the hexagonal hydroxyapatite(HAp) which has center of symmetry of crystal. However, in recent years, the piezoelectric property of artificially synthesized HAp was reported. In the authors' previous report, a new result which showed the piezoelectricity of the hydroxyapatite(HAp) films fabricated by the pulsed laser deposition(PLD) method was reported. In this study, the effect of poling treatment on piezoelectric constant of pulsed laser deposited HAp films was investigated.
  • Moving-mask lithography for 3D microstructure molding, Nobuhiro Kato, Takahisa Kai, Masakazu Hirano, Journal of Photopolymer Science and Technology, Journal of Photopolymer Science and Technology, 27(1), 85 - 89, Aug. 08 2014 , Refereed
    Summary:© 2014SPST. Backside lithography with a moving mask UV exposure technique is proposed. A novel moving-mask exposure apparatus was developed and evaluated both experimentally and through exposure simulations. The backside exposure using this technique was validated and is capable of producing tapered microstructures of thick photoresist for molding. The shape of the structure can be modified by the trajectory of the stage movement. It was confirmed that the shape of the processed structure could be successfully predicted using the proposed simulation method.
  • Moving-mask Lithography for 3D Microstructure Molding, Nobuhiro Kato, Takahisa Kai, Masakazu Hirano, JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 27(1), 85 - 89, 2014 , Refereed
    Summary:Backside lithography with a moving mask UV exposure technique is proposed. A novel moving-mask exposure apparatus was developed and evaluated both experimentally and through exposure simulations. The backside exposure using this technique was validated and is capable of producing tapered microstructures of thick photoresist for molding. The shape of the structure can be modified by the trajectory of the stage movement. It was confirmed that the shape of the processed structure could be successfully predicted using the proposed simulation method.
  • Adhesive strength between flexible hydroxyapatite sheet and tooth enamel, E. Yamamoto, N. Kato, H. Nishikawa, M. Kusunoki, T. Hayami, K. Yoshikawa, S. Hontsu, Key Engineering Materials, Key Engineering Materials, 529-530(1), 522 - 525, 2013 , Refereed
    Summary:Tooth enamel cannot be reconstructed once it is destroyed immoderately. Hydroxyapatite (HAp) thin sheet can potentially be used for a novel dental biomaterial to repair the enamel. Using a pulsed laser deposition (PLD) method, we have successfully created a flexible HAp sheet of less than a few micrometers in thickness. Due to its flexibility, the HAp sheet is tightly adhered on curved surfaces at the target site. In the present study, we newly developed double-layered sheets composed of HAp film coated with tricalcium phosphate (TCP) thin layer. The HAp/TCP sheet was adhered to the extracted human teeth using a calcium phosphate solution for 3 days. The adhesive strength between the HAp/TCP sheet and tooth enamel was evaluated by quasi-static tensile tests. Moreover, the interface structure between them was observed by a scanning electron microscopy. As a result of the mechanical evaluation, the adhesive strength was greater than approximately 2.5 MPa. The electron microscopic observation revealed that the sheet was partially fused with the enamel. These findings suggest the possibility that enamel defects are repaired using the HAp/TCP sheet for a short duration. © (2013) Trans Tech Publications, Switzerland.
  • Selection of high-potential embryos by culture in poly(dimethylsiloxane) microwells and time-lapse imaging, Shu Hashimoto, Nobuhiro Kato, Kazuhiro Saeki, Yoshiharu Morimoto, Fertility and Sterility, Fertility and Sterility, 97(2), 332 - 337, Feb. 2012 , Refereed
    Summary:Objective: To assess the developmental kinetics of human embryos and their ability to develop to morphologically normal blastocysts. Design: Experimental study on human embryos donated for research using a time-lapse imaging system based on individual embryo culture in poly(dimethylsiloxane) microwells and monitored using a microscope inside the incubator. Setting: Private fertility clinic. Patient(s): Surplus embryos donated by couples after undergoing fertility treatment. Intervention(s): None. Main Outcome Measure(s): Blastocyst score and times required from beginning to completion of the second and third mitotic divisions. Result(s): The time required for completion of the second division (the three- to four-cell stage) was shorter in embryos that developed to high-scoring blastocysts (0.7 hours, n = 17) than in those forming low-scoring blastocysts (3.7 hours, n = 24). Similarly, the mean time required to completion of the third division (five- to eight-cell stage) was also significantly shorter in embryos forming high-scoring blastocysts (5.7 hours) than among those forming low-scoring blastocysts (16.9 hours). Conclusion(s): Individual embryos with the potential to develop to high-scoring blastocysts could be selected at 2-3 days of culture using this system by examining the times required to complete the second and third mitotic divisions. © 2012 American Society for Reproductive Medicine, Published by Elsevier Inc.
  • Regeneration of tooth enamel by flexible hydroxyapatite sheet, S. Hontsu, N. Kato, E. Yamamoto, H. Nishikawa, M. Kusunoki, T. Hayami, K. Yoshikawa, Key Engineering Materials, Key Engineering Materials, 493-494, 615 - 619, 2012 , Refereed
    Summary:Before a tooth erupts, the ameloblasts are lost, which means that the tooth enamel does not regenerate itself after tooth eruption. In the present study, we attempt to regenerate the tooth enamel artificially using a flexible hydroxyapatite (HAp) sheet. First, a HAp film was deposited on a soluble substrate by pulsed laser deposition (PLD) using an ArF excimer laser. Next, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. The HAp sheet was adhered to the extracted human teeth using a calcium phosphate solution. The variation of the crystal structure of the HAp sheet with time was investigated by X-ray diffraction analysis. Furthermore, the variation in the mechanical characteristics with time between the HAp sheet and dental enamel were evaluated using tensile and scratch tests. The results suggest that the HAp sheet became fused to the tooth enamel within approximately one week. © (2012) Trans Tech Publications.
  • Effects of Volume of Poly-(dimethylsiloxane) Microwells and Number of Embryos in Wells on In-Vitro Early Bovine Development, Kazuhiro Saeki, Nobuhiro Kato, Daisaku Iwamoto, Junki Sho, Masao Kishi, Kazuya Matsumoto, Yoshihiko Hosoi, Akira Iritani, BIOLOGY OF REPRODUCTION, BIOLOGY OF REPRODUCTION, 85, Jul. 2011 , Refereed
  • Restoration and conservation of dental enamel using a flexible apatite sheet, S. Hontsu, K. Yoshikawa, N. Kato, Y. Kawakami, T. Hayami, H. Nishikawa, M. Kusunoki, K. Yamamoto, Journal of the Australian Ceramic Society, Journal of the Australian Ceramic Society, 47(1), 11 - 13, 2011 , Refereed
    Summary:A freestanding hydroxyapatite (HAp) sheet of 5 ∼ 10 μm thickness was successfully prepared by a novel method. After depositing the thin HAp film on a soluble substrate by pulsed laser deposition (PLD) technique, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. Thereafter, HAp sheets were directly attached to enamel and dentin surfaces of extracted human teeth. Artificial saliva was sprayed every third day, and the course was observed for 90 days. Observation of the enamel-HAp and dentin-HAp interface by scanning electron microscopy revealed that the HAp sheet had stuck to the enamel and dentin surface.
  • Restoration and Conservation of Dental Enamel using a Flexible Apatite Sheet, S. Hontsu, K. Yoshikawa, N. Kato, Y. Kawakami, T. Hayami, H. Nishikawa, M. Kusunoki, K. Yamamoto, JOURNAL OF THE AUSTRALIAN CERAMIC SOCIETY, JOURNAL OF THE AUSTRALIAN CERAMIC SOCIETY, 47(1), 11 - 13, 2011 , Refereed
    Summary:A freestanding hydroxyapatite (HAp) sheet of 5 similar to 10 mu m thickness was successfully prepared by a novel method. After depositing the thin HAp film on a soluble substrate by pulsed laser deposition (PLD) technique, the HAp film was collected as a freestanding sheet by dissolving the substrate using a solvent. Thereafter, HAp sheets were directly attached to enamel and dentin surfaces of extracted human teeth. Artificial saliva was sprayed every third day, and the course was observed for 90 days. Observation of the enamel-HAp and dentin-HAp interface by scanning electron microscopy revealed that the HAp sheet had stuck to the enamel and dentin surface.
  • Individually programmable cell stretching microwell arrays actuated by a Braille display, Yoko Kamotani, Tommaso Bersano-Begey, Nobuhiro Kato, Yi Chung Tung, Dongeun Huh, Jonathan W. Song, Shuichi Takayama, Biomaterials, Biomaterials, 29(17), 2646 - 2655, Jun. 2008 , Refereed
    Summary:Cell culture systems are often static and are therefore nonphysiological. In vivo, many cells are exposed to dynamic surroundings that stimulate cellular responses in a process known as mechanotransduction. To recreate this environment, stretchable cell culture substrate systems have been developed, however, these systems are limited by being macroscopic and low throughput. We have developed a device consisting of 24 miniature cell stretching chambers with flexible bottom membranes that are deformed using the computer-controlled, piezoelectrically actuated pins of a Braille display. We have also developed efficient image capture and analysis protocols to quantify morphological responses of the cells to applied strain. Human dermal microvascular endothelial cells (HDMECs) were found to show increasing degrees of alignment and elongation perpendicular to the radial strain in response to cyclic stretch at increasing frequencies of 0.2, 1, and 5 Hz, after 2, 4, and 12 h. Mouse myogenic C2C12 cells were also found to align in response to the stretch, while A549 human lung adenocarcinoma epithelial cells did not respond to stretch. © 2008 Elsevier Ltd. All rights reserved.
  • Cooperation between an enterprise and a school in mechanical design education by using 3D-CAD, Masao Fujii, Nobuhiro Kato, Takaki Shimizu, Kenichi Satomi, Shinya Taguchi, Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C, Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C, 73(1), 30 - 35, Jan. 2007 , Refereed
    Summary:3 D-CAD system has been used in practical product design to realize simultaneous engineering and also to improve productivity. Although 3 D-CAD is a widely used and highly effective tool in mechanical design, it also has its drawbacks : mastery of 3 D-CAD skills is rather complex and time-consuming. In an attempt to simplify the learning process and cut down on learning time, we have developed training programs for mechanical design such as a concept making, a family tree and a team design method, and e-Learning contents for the 3 D-CAD operation skills. The effectiveness of these training program and e-Learning contents has been confirmed by cooperative activity between an enterprise and a school in mechanical design education.
  • Improvement of polymer micro cantilevers for force controlled atomic force microscope, Nobuhiro Kato, Isao Kohno, Hisao Kikuta, Korea Research Institute of Standards and Science (KRISS) - Asia-Pacific Symposium on Measurement of Mass, Force and Torque, APMF 2005, Korea Research Institute of Standards and Science (KRISS) - Asia-Pacific Symposium on Measurement of Mass, Force and Torque, APMF 2005, 95 - 98, 2005 , Refereed
    Summary:We report on the improvement in the fabrication process of the polymer micro cantilevers for force controlled atomic force microscope. In the previous paper we reported on the preliminary results of fabrication of the micro cantilevers using SU-8, the photo resist for MEMS. The problem in conventional fabrication process are the probe tip mold failure, mask sticking and time consuming dissolving process of sacrifice layer. To overcome these difficulties we improved mask pattern for probe tip, cantilever and base. The mask pattern for probe tip is changed form square to circle. Using several sizes of circles we estimate the effect of under cut of anisotropic etching by TMAH. The mask sticking is eliminated by refined heat treatment after the soft baking process. To accelerate the sacrifice layer etching, the base and cantilever mask pattern is perforated. The sacrifice layer material is also changed. We find that the 0.6 μm thick gold on 30nm thick chromium is the appropriate material for sacrifice layer. Using this alternative process, we developed the fully plastic micro cantilevers with good probe reproducibility.
  • Polymer micro cantilevers for force controlled atomic force microscopy 3 - Improvement of fabrication process, N. Kato, C. S. Park, T. Matsumoto, H. Kikuta, K. Iwata, Proceedings of the SICE Annual Conference, Proceedings of the SICE Annual Conference, 1851 - 1854, 2004 , Refereed
    Summary:In this paper, we report on the improvements in fabrication processes of polymer micro cantilevers for force controlled atomic force microscopy. The improvement of photo mask patterns results in the enhancement of controllability of the pyramidal probe tip geometry and in rapid dissolving of sacrificial layer. It also contributes to process simplicity.
  • Fabrication of polymer cantilevers for force controlled atomic force microscope, Nobuhiro Kato, Choong Sik Park, Toshiro Matsumoto, Hisao Kikuta, Koichi Iwata, Proceedings of SPIE - The International Society for Optical Engineering, Proceedings of SPIE - The International Society for Optical Engineering, 5183, 71 - 78, 2003 , Refereed
    Summary:We developed the fabrication process of all-plastic cantilever for force controlled atomic force microscope. The force controlled atomic force microscope can prevent damage during the scanning of the soft sample. However it has a drawback in its stability. To stabilize the system the cantilever is required to have small Q factor. The negative photo resist for MEMS (SU-8) has ideal properties, micromachinability and small Q factor, for this purpose. Here we demonstrate the cantilever consists of tip, lever and base with same polyimide. The mold of the probing tip is the pyramidal pit pattern on silicon wafer formed by direct leaser writing and anisotropic etching of silicon. Other elements of the cantilever are formed by contact lithography. The photo mask is also fabricated by direct laser writing. By arranging the dimensions of the cantilever, we can control its spring constant and resonant frequency. For the spring constant of 0.1N/m and the first resonant frequency of 2kHz, the typical dimensions of the cantilever is 1030 × 300 × 7 μm3. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • Imaging polarimetry with an micro-retarder array, H Kikuta, K Numata, H Arimitsu, K Iwata, N Kato, SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5, SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5, 2510 - 2511, 2002 , Refereed
    Summary:We developed a 92x70 micro retarder array for an imaging polarimetry. The micro retarder array consists of polyimide subwavelength-period gratings of 300nm period with different orientations. The phase retardance is caused by the form birefringence of the subwavelength structure. The gratings were fabricated with the direct-writing electron-beam lithography and the reactive ion etching. The phase retardance was 0.5pi for the 550nm light wavelength. We have observed a birefringence image of a tensed plastic film by using the imaging polarimetry system.
  • Polymer micro cantilevers for force controlled atomic force microscopy 2 - Fabrication of cantilevers, N Kato, CS Park, T Matsumoto, H Kikuta, K Iwata, SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5, SICE 2002: PROCEEDINGS OF THE 41ST SICE ANNUAL CONFERENCE, VOLS 1-5, 1033 - 1034, 2002 , Refereed
    Summary:The micro cantilever made of entirely plastic material has been developed. The probing tip, cantilever and base are made of same photo plastic. By arranging the geometry of the cantilever, its spring constant and resonant frequency is controlled. The low Q of the polyimide improves the stability of the force controlled atomic force microscope. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • Electrochemical nanolithography on amorphous WO3 thin films using scanning tunneling microscope in air, Nobuhiro Kato, Toshiro Matsumoto, Hisao Kikuta, Yasuhiro Nakamura, Koichi Iwata, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 40(11), 6589 - 6593, Nov. 2001 , Refereed
    Summary:Tungsten oxide (WO3) thin films have shown interesting properties as lithography resist materials. In this study, the scanning tunneling microscope (STM) was used in air for nanometer etching of α-WO3-x thin films, an n-type semiconductor. The current-voltage (I-V) curve was found to be affected by the water layer absorbed on the surface. For low voltage (< 3 V) with long duration (∼ 10s) or high voltage pulse (> 3 V) with short pulsewidth (∼ 200 ms), holes were formed on the film surface at high humidity (> 70%) by applying a pulse voltage. A threshold voltage existed for hole formation. Higher pulse voltage and negative polarity corresponded to larger modified size. All the structures formed in STM images were topographical in nature by comparison with the AFM images. The hole formation was reasonably attributed to electrochemistry and high dissolution of WO3 in high pH solutions, which was co-manifested by links between the holes and eroded materials on the surface. Alkaline solutions instead of deionized water were chosen to act in the tip-surface gap. Alkaline ions being expelled from the tip due to electric polarity demonstrated the ion movement and their effect on etching. Lines of nanometer width were fabricated.
  • Reduction in feedback bandwidth of the force-controlled atomic force microscope using a polyimide cantilever, N Kato, T Matsumoto, H Kikuta, Y Nakamura, K Iwata, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 40(11), 6594 - 6599, Nov. 2001
    Summary:In this paper we present a method of reducing the bandwidth required in the force-controlled atomic force microscope (AFM) for measuring the force curves. System stability strongly depends on the quality factor of vibration (Q) of the cantilever. As polyimide has a low Q. a cantilever fabricated from it reduces the bandwidth required in the feedback system. A polyimide cantilever with dimensions of 540 x 155 x 3.4 mum(3) is fabricated to evaluate its Q in vacuum. density and Young's modulus. The measured Q is 50, which is much smaller than those of conventional cantilevers used for AFM. Using the measured mechanical properties of the polyimide, the required frequency response of the feedback system is estimated. Typical force curve measurements are confirmed by some numerical simulations.
  • Holographic deformation analysis of the human femur, T. Matsumoto, A. Kojima, N. Kato, T. Watanabe, M. Tamiwa, M. Baba, Annual International Conference of the IEEE Engineering in Medicine and Biology-Proceedings, Annual International Conference of the IEEE Engineering in Medicine and Biology-Proceedings, 2, 1508 - 1510, 2001 , Refereed
    Summary:The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1) the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • Holographic deformation analysis of the human femur, T Matsumoto, A Kojima, T Watanabe, M Tamiwa, M Baba, PROCEEDINGS OF THE 23RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-4, PROCEEDINGS OF THE 23RD ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-4, 23, 1508 - 1510, 2001 , Refereed
    Summary:The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1)the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • System analysis of the force-feedback method for force curve measurements, Nobuhiro Kato, Hisao Kikuta, Takashi Nakano, Toshiro Matsumoto, Koichi Iwata, Review of Scientific Instruments, Review of Scientific Instruments, 70(5), 2402 - 2407, 1999 , Refereed
    Summary:The force-feedback method is a promising technique to measure accurate force curves in atomic force spectroscopy. In this article, we describe criteria of the stability for the force-feedback system containing a proportional amplifier with first-order lag. We derive the criteria from an equation of the cantilever movement with higher-order vibration modes under force interaction. The criteria predict that a proportional amplifier with a certain gain and a "very high" cutoff frequency is required to stabilize the feedback system when the critical force gradient exceeds the cantilever stiffness. To measure a force curve, including the steep part (-17 N/m) with a soft (0.2 N/m) and poor-damping (2 X 10-7 kg/s) cantilever, the amplifier for the force-feedback controller must have a frequency response wider than 16 MHz and gain larger than 16.8. Moreover, the feedback controller with only an integrator, which is popular in scanning tunneling microscopy and atomic force microscopy, does not contribute to reducing the discontinuity of the force curve. © 1999 American Institute of Physics.
  • Formation of Biocompatible Hydroxyapatite Thin Films deposited by Laser Ablation (共著), Bio ceramics (Proc. 12th Int. Sympo. on Ceramics in Medicine), Bio ceramics (Proc. 12th Int. Sympo. on Ceramics in Medicine), 12, 499 - 502, 1999
  • Resonance characteristics of micro cantilever in liquid, Y Hirai, R Mori, H Kikuta, N Kato, K Inoue, Y Tanaka, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS, 37(12B), 7064 - 7069, Dec. 1998
    Summary:The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also. the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results.
  • Formation of hydroxyapatite thin films on surface-modified polytetrafluoroethylene substrates, Shigeki Hontsu, Masaya Nakamori, Nobuhiro Kato, Hitoshi Tabata, Junya Ishii, Toshirou Matsumoto, Tomoji Kawai, Japanese Journal of Applied Physics, Part 2: Letters, Japanese Journal of Applied Physics, Part 2: Letters, 37(10 PART A), L1169 - L1171, Oct. 01 1998 , Refereed
    Summary:An ArF excimer laser deposition technique is used to fabricate hydroxyapatitc [Ca10(PO4)6(OH)2]-(HAp) thin films on surface-modified polytetrafluoroethylene (PTFE) substrates. The surface of PTFE is modified by a sodium-naphthalene complex in a glycol ether solvent. X-ray diffraction (XRD) studies indicate that as-deposited films prepared at substrate temperatures below the glass transition temperature of PTFE (327°C) are in an amorphous state. The crystallization of as-deposited films needed annealing for 10h at 310°C. The structure, surface morphology and bond strength of HAp films were evaluated using XRD, the atomic force microscopy (AFM) and the Instron testing machine. The tensile bond strength of HAp films on surface-modified PTFE was 6.0 MPa, which is one order of magnitude larger than that of films on non-surface-modified PTFE, demonstrating its potential for practical applications.
  • On the polymer thickener for viscosity-stabilized solar pond, Toshiro Matsumoto, Nobuhiro Kato, Masao Taga, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 37(9 A), 5088 - 5093, Sep. 1998 , Refereed
    Summary:The optimal physical properties of an ionized polyacrylamide solution, selected as the most suitable thickener for the insulating layer of a viscosity-stabilized solar pond, are described. Light transmittance, viscosity and viscosity degradation were obtained for a range of thicknesses from 1 cm to 15 cm (1-15 cm), weight concentrations of 0.1-0.5% (0.1-0.5 wt%) and temperatures of the polymer of 20-80°C. Onset of convection was also measured for a given thickness of the polymer solution. An exposure test was carried out to prevent degradation of the polymer using an antioxidant. It was confirmed that the viscosity was not decreased and, on the contrary, increased slightly after 100 days. It was shown experimentally that the critical Rayleigh number coincided with the theoretical values reported by Tien et al. [Int. J. Heat Mass Transf. 12 (1969) 1173]. Transmittances of the polymeric gel for the case of vessels greater than 5 cm in thicknesses were better than those of the SPR402 solution (0.5 wt%).
  • Resonance characteristics of micro cantilever in liquid, Y. Hirai, R. Mori, H. Kikuta, N. Kato, K. Inoue, Y. Tanaka, Digest of Papers - Microprocesses and Nanotechnology 1998: 1998 International Microprocesses and Nanotechnology Conference, Digest of Papers - Microprocesses and Nanotechnology 1998: 1998 International Microprocesses and Nanotechnology Conference, 1998-July, 89 - 90, 1998 , Refereed
  • Resonance characteristics of micro cantilever in liquid, Yoshihiko Hirai, Ryosuke Mori, Hisao Kikuta, Nobuhiro Kato, Koji Inoue, Yoshio Tanaka, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 37(12 B), 7064 - 7069, 1998 , Refereed
    Summary:The resonance characteristics of a micro cantilever are experimentally and theoretically studied. The micro cantilever is vibrated by thermal excitation using laser exposure and the frequency responses of the micro cantilever in air, acetone and water are experimentally evaluated based on an optical measurement technique. It is demonstrated that the resonance frequencies in water decrease to about one-tenth of the values in air. Also, the frequency responses are calculated using the string of beads model taking into consideration the drag force by viscous fluids. The calculation results show good agreement with the experimental results. © 1998 Publication Board, Japanese Journal of Applied Physics.
  • On the Polymer Thickener for Viscosity-Stabilized Solar Pond (共著), Toshiro Matsumoto, Nobuhiro Kato, Masao Taga, Jpn. J. Al. Phys., Jpn. J. Al. Phys., 37(9A), 5088 - 5093, 1998
  • Formation of Hydroxyapatite Thin Films on Surface-Modified Polytetrafluorothylene Substrates (共著), Jpn. J. Al. Phys., Jpn. J. Al. Phys., 37(1), 9A,1169-1171, 1998
  • Force-balancing microforce sensor with an optical-fiber interferometer, Nobuhiro Kato, Ippei Suzuki, Hisao Kikuta, Koichi Iwata, Review of Scientific Instruments, Review of Scientific Instruments, 68(6), 2475 - 2478, Jun. 1997 , Refereed
    Summary:A microforce sensor with a force feedback method for scanning force microscopy is presented. The force sensor is constructed by using an optical fiber and a microcantilever. The facet of the optical fiber is coated with a gold thin film 15 Å thick. This gold film acts not only as a partially reflected mirror but also as an electrode of the electrostatic actuator. The interaction force between a probe tip and a sample is balanced by the electrostatic force. The deflection of the cantilever is measured by an interferometer consisting of the facet of the optical fiber and the cantilever surface. We have made the force sensor and measured some force curves of a mica surface with a SiN probe tip. The force curves obtained by this sensor are quite different from the curves by a conventional measurement without a feedback system. The force resolution of the system is 10-10 N with a bandwidth of dc∼1 kHz. © 1997 American Institute of Physics.
  • Deformation measurement of lumber vertebra by using holographic interferometry (共著), Memoirs of THE SCHOOL OF BIO-ORIENTED SCI. & TECHNOL. OF KINKI UNIV., Memoirs of THE SCHOOL OF BIO-ORIENTED SCI. & TECHNOL. OF KINKI UNIV., (2), 50 - 58, 1997
  • Force-balancing force sensor with an optical lever, Nobuhiro Kato, Ippei Suzuki, Hisao Kikuta, Koichi Iwata, Review of Scientific Instruments, Review of Scientific Instruments, 66(12), 5532 - 5536, 1995 , Refereed
    Summary:Scanning force microscopes (SFMs) are sometimes used to obtain a force curve, which shows the force variation as a function of tip-sample distance. In the force curve measurement, if the spring constant of the force detecting lever is small, the measured force curve has discontinuity and is different from the true force curve. In this paper, we present a new type of force balancing force sensor built in SFM. This force sensor employs an optical lever for detecting the rotation of the lever and two electrostatic force actuators with transparent electrodes. This sensor has two operating modes; with and without feedback. In the feedback mode, the force detecting lever is balanced with the electrostatic force. This system has the effect of enlarging the effective spring constant of the whole sensor. In the nonfeedback mode, this sensor acts as an ordinary force sensor. By using this sensor in both modes we will show the effectiveness of the force feedback in force curve measurements. © 1995 American Institute of Physics.
  • Atomic force microscope using optical heterodyne detection incorporated in an optical microscope, Hisao Kikuta, Kosuke Nasu, Nobuhiro Kato, Koichi Iwata, Review of Scientific Instruments, Review of Scientific Instruments, 66(1), 87 - 90, 1995 , Refereed
    Summary:An atomic force microscope has been constructed which enables us to observe sample surfaces through a metallurgical optical microscope. The cantilever deflection is detected by a polarization common-path interferometer with optical heterodyne technique. The optical system of the interferometer is incorporated in the commercial optical microscope. The common-path interferometer is rather insensitive to the effects of environmental disturbance such as thermal drift and mechanical vibration. The sample surface and the cantilever are observed by a microscopic objective of ×20. We can adjust the interferometer easily by observing both the cantilever and laser beams. The detection system has a total rms noise of 0.35 Å in a frequency bandwidth of 0.5-200 Hz on a free Si3N4 cantilever of 0.1 N/m spring constant. © 1995 American Institute of Physics.

Books etc

  • Advanced Technologies of Cell Separation and Manipulation, Joint author,   2008 04

Conference Activities & Talks

  • Feasibility study to recognize cleavage of bovine early embryo based on observation image analysis by deep convolutional neural network, 高橋 鷹兵, 根本 充貴, 加藤 暢宏, 朴 忠植, 山東 悠介, 佐伯 和弘, システム制御情報学会研究発表講演会講演論文集,   2019 05 22
  • 3Dプリンタで作成したポリ乳酸製メッシュ状マイクロニードル基部の柔軟性測定, 千原なみえ, 坂本佳奈子, 森中杏菜, 植田留名, 萬屋沙織, 加藤暢宏, 精密工学会大会学術講演会講演論文集,   2018 08 20
  • メッシュ状マイクロニードルのための多波長逐次露光型移動マスク露光, 坂本佳奈子, 千原なみえ, 森中杏菜, 植田留名, 加藤暢宏, 精密工学会大会学術講演会講演論文集,   2018 08 20
  • Microneedles fabricated by heat imprinting for novel application, KATO Nobuhiro, Microneedles2018,   2018 05 29 , 招待有り
  • 脱灰・再石灰化環境内での極薄非晶質リン酸カルシウム人工エナメル質シートの安定性, 平井 瑞樹, 本津 茂樹, 山本 衛, 加藤 暢宏, 日本歯科理工学会誌,   2018 03
  • 熱インプリントによるマイクロニードルアレイの作成, 加藤暢宏, 千原なみえ, 坂本佳奈子, 森中杏菜, 精密工学会大会学術講演会講演論文集,   2018 03 01
    Summary:

    移動マスク露光法により得られたマイクロニードルのフォトレジスト型を原型に、シリコーンエラストマーを用いてレプリカ型を得た。このレプリカ型に対して生体適合性のある熱可塑性樹脂を熱インプリントすることでマイクロニードルアレイを得ることができた。熱インプリントに先立って熱可塑性樹脂をあらかじめ所望の形状に整形しておくことでマイクロニードルアレイの機械的特性を制御できることが示唆された。

  • 椎間板組織再生のための細胞低接着性コラーゲンゲルの開発, 森本康一, 國井沙織, 武岡由樹, 由留部崇, 加藤暢宏, 尾前薫, 黒田良祐, 日本バイオマテリアル学会大会予稿集(Web),   2018
  • 細胞低接着性コラーゲンによる神経細胞の活性化と組織再生機能の検証, 國井沙織, 兼清健志, 中野法彦, 加藤暢宏, 尾前薫, 井出千束, 森本康一, 日本バイオマテリアル学会大会予稿集(Web),   2018
  • 細胞低接着性コラーゲンを培養足場とした線維芽細胞スフェロイドの遺伝子発現量の変化, 森本康一, 國井沙織, 伊田寛之, 平岡陽介, 加藤暢宏, 日本バイオマテリアル学会大会予稿集(Web),   2017 11 13
  • Development of Ultra-Thin Opaque White Hydroxyapatite Sheet for Restoration of Enamel and Aesthetic Treatments of Teeth, Nobuhiro Kato, Yuki Ido, Ei Yamamoto, Shigeki Hontsu, Bioceramics 29,   2017 10
  • Adhesive Evaluation by Brushing Tests for Hydroxyapatite Films Fabricated on Dentins Using a Water Mist Assisted Er:YAG Laser Deposition Method, Ei Yamamoto, Nobuhiro Kato, Shigeki Hontsu, Bioceramics 29,   2017 10
  • Aesthetic effect of opaque white hydroxyapatite artificial enamel sheet, 平井瑞樹, 本津茂樹, 片山一郎, 加藤暢宏, 橋本典也, 吉川一志, 山本一世, 日本歯科理工学会誌,   2017 09 25
  • Photoresist Fabrication System for Microneedle Production using Syringe Dispenser Coating and Continuous Developing Circuit with Peristaltic Pump, KATO Nobuhiro, SAKAMOTO Kanako, CHIHARA Namie, MORINAKA Anna, 精密工学会学術講演会講演論文集,   2017 09 20
  • Fabrication of microneedles using sequential multi-wavelength UV exposure with moving-mask lithography, KATO Nobuhiro, MORI Shunta, Proceedings of JSPE Semestrial Meeting,   2017 03 13
    Summary:透明基板裏面からのネガ型厚膜フォトレジストへの移動マスク露光によってマイクロニードルの形成が可能である。従来は単一波長(λ=365nm)の紫外線のみを使用しており、形成できる形状に制約があった。本研究ではフォトレジストの吸光特性の測定結果に基づいて、通常は露光に使用されない複数波長の紫外線を使用することでニードル部分と土台部分の形状の自由度を飛躍的に向上させた。
  • Er:YAGレーザーデポジション法を用いたアパタイト膜形成におけるミストアシストの効果, 本津茂樹, 山本衛, 加藤暢宏, 保尾謙三, 吉川一志, 山本一世, 日本レーザー歯学会総会・学術大会プログラム・講演抄録集,   2016 12
  • Optimization of Humid Conditions Using an Ultrasonic Nebulizer for the Fabrication of Hydroxyapatite Film with the Er:YAG Laser Deposition Method, Ei Yamamoto, Nobuhiro Kato, Yuka Hatoko, Shigeki Hontsu, Bioceramics 28,   2016 11
  • Preparation and Application of a Potassium-Substituted Calcium Phosphate Sheet as a Dental Material for Treating Dentin Hypersensitivity, Kato, N, Y. Hatoko, E. Yamamoto, T. Furuzono, S. Hontsu, Bioceramics 28,   2016 11
  • 細胞低接着性コラーゲンが細胞増殖能に与える影響, 國井沙織, 加藤暢宏, 堀内喜高, 森本康一, 日本生化学会大会(Web),   2016 09
  • Arbitrary shape microneedle generation using moving-mask lithography, Nobuhiro Kato, Shunta Mori, Microneedles 2016,   2016 05 23
  • Evaluation of adherence properties between perforated ultrathin amorphous calcium phosphate sheet and dentin, 井戸雄基, 山本衛, 加藤暢宏, 保尾謙三, 吉川一志, 山本一世, 本津茂樹, 日本歯科理工学会誌,   2016 03 25
  • Preparation and evaluation of fluoridated hydroxyapatite sheets for dentin restoration, 小比賀優, 波床侑果, 加藤暢宏, 山本衛, 吉川一志, 山本一世, 本津茂樹, 日本歯科理工学会誌,   2016 03 25
  • Fabrication of apatite films using the mist-assisted Er:YAG laser deposition method, 本津茂樹, 波床侑果, 山本衛, 加藤暢宏, 吉川一志, 山本一世, 日本歯科理工学会誌,   2016 03 25
  • 開発したタイムラプスモニタリング装置によるウシ胚体外培養の観察, 藤木雄太, 加藤暢宏, 宮田静, 朴忠植, 山東悠介, 佐伯和弘, 日本はい移植学雑誌,   2016 01 29
  • Analytical and experimental study of passive regulator for micro channel, Kadota Takayuki, Katou Nobuhiro, Transactions of Japanese Society for Medical and Biological Engineering,   2016
    Summary:

    Flow rate of fluid in micro channel driven by gravity pump is proportional to the head difference. Regulator for long-term microfluidic cell culture system was needed. The conventional flow regulator systems enabling constant flow rate are not easy to miniaturize to fit on micro channels. In this study, simple passive regulator systems that can be applied to micro channel are presented. The result of FEM analysis predicted the effectiveness of deformable valve structure. The analyzed results were evaluated by experiment using microfluidic system with 4 types deformable passive valves.

  • Mechanical characteristic of biodegradable microneedles, Mori Shunta, Kai Takahisa, Susaki Yusuke, Yamamoto Ei, Kato Nobuhiro, Transactions of Japanese Society for Medical and Biological Engineering,   2016
    Summary:

    Microneedles (MNs) are transdermal drug delivery systems, making it possible to administer drugs into the dermis without pain. In this study, master male molds of MNs were obtained by moving-mask UV lithography. The MNs female molds were replicated by poly-dimethylsiloxane. Finally, MNs were obtained by casting chondroitin sulfate C sodium salt (CSC) solution to the female molds. The Young's modulus of CSC was evaluated to be 2-4 GPa by tensile test. The CSC was enough stiff to penetrate epidermis. The penetration tests were conducted to characterize various shapes of CSC-MNs.

  • Microneedles Protoryping Using Moving-Mask Lithography, Nobuhiro Kato, Takahisa Kai, Shunta Mori, 12th International Conference on Ubiquitous Healthcare,   2015 12 02
  • Adhesion Properties of an Apatite Film Deposited on Dentine Using Er:YAG Laser Ablation Methods, Ei YAMAMOTO Nobuhiro, KATO Kazushi YOSHIKAWA Kenzo, YASUO Kazuyo, YAMAMOTO Shigeki HONTSU, Bioceramics 27,   2015 10 27
  • Overlaid Ultrathin Amorphous Calcium Phosphate Sheet Improves Dentinal Permeability Inhibition Rate, Nobuhiro Kato, Yuki Ido, Ei Yamamoto, Kenzo Yasuo, Kazushi Yoshikawa, Shigeki Hontsu, Bioceramics 27,   2015 10 27
  • B203 Migration property of fibroblast cultured on an elastin-coating substrate, NISHI Shingo, SUSAKI Yusuke, KAI Takahisa, MIYATA Shizuka, NISHIMOTO Masaya, TAKEMORI Kumiko, KATO Nobuhiro, ITO Hiroyuki, YAMAMOTO Ei, Proceedings of the ... JSME Conference on Frontiers in Bioengineering,   2015 10 01
  • Microneedles fabrication utilizing a moving-mask lithography, Tkahisa KAI, Shunta MORI, Nobuhiro KATO,   2015 09 25
  • Development of embryo monitoring system using time lapse cinematography, Shizuoka MIYATA, Choongsik PARK, Yusuke SANDO, Yuta FUJIKI, Kazuhiro SAEKI, Nobuhiro KATO,   2015 09 25
  • P-72 Preparation and evaluation of potassium-containing hydroxyapatite sheet for dentin hyperesthesia treatment, Hatoko Y, Yamamoto E, Kato N, Nishikawa H, Yoshikawa K, Furuzono T, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices,   2015 09 15
    Summary:In the therapy for hypersensitivity of tooth, it is known that potassium (K) compounds are often added in the toothpaste because it is believed that neurotransmission is blocked by potassium ions. Recently, we have been proposing the attachment therapy of a hydroxyapatite (HAp) sheet on dentin for hyperesthesia. It is thought that addition of K ions in HAp sheet is more effective for relieve pain of tooth hypersensitivity. In this study, we prepared K-HAp thin film and evaluated it. K-HAp sheet was prepared by using a process for preparing thin film. After that, we evaluated the interface between K-HAp sheet and dentin.
  • Er:YAGレーザーアブレーション法で象牙質上に形成したアパタイト膜の固着特性, 本津 茂樹, 山本 衛, 加藤 暢宏, 保尾 謙三, 吉川 一志, 山本 一世, 日本レーザー歯学会誌,   2015 08
  • 極薄非晶質リン酸カルシウムシート重ね貼りによる象牙細管の封鎖効果, HONTSU SHIGEKI, IDO YUKI, YAMAMOTO EI, KATO NOBUHIRO, YASUO KENZO, YOSHIKAWA KAZUSHI, YAMAMOTO KAZUYO, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web),   2015 06 01
  • Development of embryo monitoring system using time lapse cinematography, MIYATA SHIZUKA, PARK CHOONGSIK, SANDO YUSUKE, SAEKI KAZUHIRO, KATO NOBUHIRO, Transactions of Japanese Society for Medical and Biological Engineering,   2015 05 07
    Summary:A simple time-lapse cinematography device that provides useful insight for embryo quality scoring by series observation of embryos during culture was developed. The device consists of a microscope system, a PC controlled camera and an illumination system. All components are implemented in a waterproof enclosure to be operated inside a CO2 incubator. Operations for image capture including focus adjustment and illumination adjustment can be remotely performed using PC without removing embryos from the optimal gas and temperature conditions. Using this device, clear images including several embryos were captured and the cleavage timing of embryos can be exactly identified.
  • P-40 Evaluation on the effect of lap-strake perforated amorphous calcium phosphate sheets on the sealing of dentinal tubules, Ido Y, Isai A, Yamamoto E, Kato N, Nishikawa H, Yasuo K, Hashimoto Y, Yoshikawa K, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices,   2015 03 25
    Summary:We propose a new method to shield dentinal tubules by direct attaching an amorphous calcium phosphate (ACP) sheet on the dentin as a treatment of the hyperaesthesia. In this study, we fabricated the perforated ACP sheet in order not only to easily remove liquid on the dentinal surface but also to promote the remineralization at the interface between the sheet and dentin. The ACP sheet was prepared by the pulsed laser deposition technique and the laser process using a metal mask. The interface structure between the ACP sheet and dentin was observed by a SEM. In addition, using Pashle's method, we evaluated the inhibition effect of lap-strake perforated ACP sheets attached to the dentin on its permeability.
  • GnRH‐FSH処理が黒毛和種雌牛のOPUに及ぼす影響, TANIGUCHI SHUNJI, TAKADA HIROTATSU, TARUMOTO HIDEYUKI, KATO NOBUHIRO, SAEKI KAZUHIRO, 日本はい移植学雑誌,   2015 01 21
  • Formation of self degradable microneedle array using moving mask lithography, KAI TAKAHISA, MORI SHUNTA, KATO NOBUHIRO, Transactions of Japanese Society for Medical and Biological Engineering,   2015
    Summary:A moving-mask exposure apparatus that allows backside exposure through transparent substrate to form the mold of microneedle patch with thick photoresist was developed. By choosing moving trajectory, various shape microneedle can be produced from a single mask pattern. The photoresist microneedle pattern was replicated by poly-dimethylsiloxane (PDMS). The mixture of chondroitin sulfate C and sterilized water was poured over the PDMS mold. Finally, the cured chondroitin sulfate C microneedle patch was detached from the PDMS mold. From scanning microscope observation, successful shape transfer form the photoresist MN to the chondroitin sulfate C microneedle was confirmed.
  • Evaluation of Dentin Tubule Sealing Rate Improved by Attaching Ultrathin Amorphous Calcium Phosphate Sheet, Kato, Nobuhiro Isai, Arata Yamamoto, Ei Nishikawa, Hiroaki Kusunoki, Masanobu Yoshikawa, Kazushi Yasuo, Kenzo Yamamoto, Kazuyo Hontsu, Shigeki, Bioceramics 26,   2014 11 06
  • A Novel Treatment for Dentine Cavities with Intraoral Laser Ablation Method Using an Er:YAG Laser, Yamamoto, Ei Kato, Nobuhiro Isai, Arata Nishikawa, Hiroaki Hashimoto, Yoshiya Yoshikawa, Kazushi Hontsu, Shigeki, Bioceramics 26,   2014 11 06
  • マイクロニードル型作製のための移動マスク露光法, KAI TAKAHISA, HIRANO MASAKAZU, KATO NOBUHIRO, 日本機械学会マイクロ・ナノ工学シンポジウム講演論文集(CD-ROM),   2014 10 13
  • B112 Fibroblast culture using an elastin-coated dish, NISHI Shingo, SUSAKI Yusuke, MIYATA Shizuka, TAKEDA Ippei, TAKEMORI Kumiko, KATO Nobuhiro, ITO Hiroyuki, YAMAMOTO Ei, Proceedings of the ... JSME Conference on Frontiers in Bioengineering,   2014 10 02
  • Er:YAGレーザーアブレーション法によって形成されたアパタイト膜の象牙細管封鎖効果, HONTSU SHIGEKI, YAMAMOTO EI, KATO NOBUHIRO, NISHIKAWA HIROAKI, YASUO KENZO, YOSHIKAWA KAZUSHI, YAMAMOTO KAZUYO, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web),   2014 10 01
  • P-16 Preparation and evaluation of ultra thin potassium-doped hydroxyapatite sheet, Hatoko Y, Yamamoto E, Kato N, Nishikawa H, Yoshikawa K, Furuzono T, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices,   2014 09 20
    Summary:In toothpaste formulated for hypersensitivity, potassium compounds such as potassium nitrate and potassium bromide are often added because it is believed that neurotransmission is blocked by the function of potassium ions. Thus far, we have proposed attaching a hydroxyapatite sheet on dentin as a treatment for hyperesthesia. We think that it may be possible to block dental tubules and relieve pain by adding potassium to the sheet for sustained release. In this study, we prepared and evaluated a potassium-doped hydroxyapatite thin film, and applied the process to the preparation of a potassium-doped hydroxyapatite sheet.
  • P-15 Evaluation of the dentin permeability inhibition effect by lap-strake method of the amorphous calcium phosphate sheet, Isai A, Yamamoto E, Kato N, Nishikawa H, Yasuo K, Hashimoto Y, Yoshikawa K, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices,   2014 09 20
    Summary:We have fabricated a flexible hydroxyapatite (HAp) sheet using pulsed laser deposition and a sheet isolation technique. We aim to form an artificial enamel layer on the dentin by an amorphous calcium phosphate (ACP) sheet with solubility higher than compared that of the crystallized sheet. In this study, using Pashle's method, we evaluated of the dentin permeability inhibition effect by double layer method of the ACP sheet on dentin as a cure for dental hypersensitivity. The dentin permeability inhibition rate of the dentinal tubule by double layer method of the ACP sheet was 89.17±7.24%. The ACP sheet was yielded results better than of dental resin cement.
  • 多層非晶質リン酸カルシウムシート貼付法による象牙質透過抑制率効果の評価, 以西 新, 山本 衛, 加藤 暢宏, 西川 博昭, 保尾 謙三, 橋本 典也, 吉川 一志, 山本 一世, 本津 茂樹, 日本歯科理工学会誌,   2014 09
  • 極薄カリウム含有ハイドロキシアパタイトシートの作製と評価, 波床 侑果, 山本 衛, 加藤 暢宏, 西川 博昭, 吉川 一志, 古薗 勉, 山本 一世, 本津 茂樹, 日本歯科理工学会誌,   2014 09
  • パルスレーザー堆積法によるカリウム含有ハイドロキシアパタイト薄膜の作製, HATOKO YUKA, YAMAMOTO EI, KATO NOBUHIRO, NISHIKAWA HIROAKI, FURUZONO TSUTOMU, HONTSU SHIGEKI, 日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM),   2014 08 25
  • Moving-mask Lithography for 3D Microstructure Molding, Nobuhiro Kato, Takahisa Kai, Masakazu Hirano, ICPST-31,   2014 07 08
  • Er:YAGレーザー成膜法による歯質上へのアパタイト膜の直接形成, 本津 茂樹, 山本 衛, 加藤 暢宏, 西川 博昭, 吉川 一志, 山本 一世, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集,   2014 06
  • A-18 Examination of dentine restoration using the Er:YAG laser ablation method (Part1), Hontsu S, Yamamoto E, Kato N, Nishikawa H, Hashimoto Y, Yoshikawa K, Yamamoto K, The journal of the Japanese Society for Dental Materials and Devices,   2014 03 25
    Summary:A calcium phosphate film was deposited on the dentine in the air by applying Er:YAG laser to the laser ablation (PLD) method to try the restoration of the dentine. α-tricalcium phosphate (α-TCP) was used as a target for the film preparation, and cattle enamel and human dentin were used as a substrate. X-ray diffraction (XRD) technique was used for crystallinity evaluation of the deposited layer, and scanning electron microscope (SEM) was used for the evaluation of the substrate-deposited layer interface.
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養, 岩本 太作, 谷口 俊仁, 岸 昌生, 加藤 暢宏, 佐伯 和弘, 日本胚移植学雑誌,   2014 01
  • 極薄アパタイトシートを用いた象牙細管の封鎖効果についての検討(第2報), 本津 茂樹, 以西 新, 山本 衛, 加藤 暢宏, 西川 博昭, 吉川 一志, 山本 一世, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集,   2013 10
  • 極薄アパタイトシートを用いた象牙細管の封鎖効果についての検討, 以西 新, 山本 衛, 加藤 暢宏, 西川 博昭, 吉川 一志, 山本 一世, 本津 茂樹, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集,   2013 05
  • A-17 Examination of enamel repairing method using ultrathin apatite sheet (Part2), Hontsu S, Yoshikawa K, Isai A, Yamamoto E, Kato N, Nishikawa H, Kusunoki M, Hashimoto Y, Yamamoto K, The journal of the Japanese Society for Dental Materials and Devices,   2013 03 25
    Summary:We have successfully created a flexible hydroxyapatite (HAp) sheet of less than a few micrometers, and we have been trying to establish the all-HAp restoration technique for tooth enamel. In this study, we evaluated the re-calcification layer at the various sheets (amorphous HAp, HAp, amorphous HAp/amorphous calcium phosphate: ACP, HAp/ACP)-enamel interface by scanning electron microscope (SEM).
  • 穴あきアパタイトシートを用いたエナメル質の修復, 本津 茂樹, 以西 新, 山本 衛, 加藤 暢宏, 西川 博昭, 楠 正暢, 速水 尚, 吉川 一志, 日本バイオマテリアル学会大会予稿集,   2012 11
  • Adhesive Strength Between Flexible Hydroxyapatite Sheet and Tooth Enamel, E. Yamamoto, N. Kato, H. Nishikawa M, Kusunoki T, Hayami S. Hontsu, Bioceramics 24,   2012 10 21
  • 穴あき極薄アパタイトシートによるエナメル質の修復, 本津 茂樹, 以西 新, 山本 衛, 加藤 暢宏, 西川 博昭, 吉川 一志, 山本 一世, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集,   2012 10
  • B-28 Formation of the artificial enamel on dentin using the ultrathin apatite sheet, Hontsu S, Yoshikawa K, Isai A, Yamamoto E, Kato N, Nishikawa H, Kusunoki M, Hashimoto Y, Yamamoto K, The journal of the Japanese Society for Dental Materials and Devices,   2012 09 25
    Summary:We have fabricated a flexible hydroxyapatite (HAp) using a pulsed laser deposition (PLD) method and a film isolation technique. We adhered the HAp sheet to the dentine surface using a calcium phosphate aqueous in order to apply this sheet to hyperesthetic treatment. In the present study, the time dependence of the composition at the interfaces between the HAp/TCP sheet and the dentine was evaluated using X-ray diffraction measurement, and the adhesive strength between the HAp/TCP sheet and the dentine surface was evaluated by quasi-static tensile tests.
  • K11300 Education of Design from Scratch, Matsubara Atsushi, Kogiso Nozomu, Kato Nobuhiro, Yokokoji Yasuyoshi, Tutsui Shinsaku, Makino Osamu, Mechanical Engineering Congress, Japan,   2012 09 09
    Summary:An education method for mechanical design is discussed. In conventional design education, the prototype designs together with design process are given for students, which can be referred to as parameterized design. This is a good introduction for students to start the design class. However, the big picture of the design process could not be learned. For students to experience total design paradigm, a design seminar has been opened every summer in past decade. We discuss the pros and cons of the current seminar and present the perspective of next turn.
  • P-31 Examination of enamel repairing method using ultrathin apatite sheet(Part1), Hontsu S, Yoshikawa K, Matsuda T, Kato N, Nishikawa H, Kusunoki M, Hashimoto Y, Yamamoto K, The journal of the Japanese Society for Dental Materials and Devices,   2012 03 25
    Summary:We have succeeded to fabricate flexible hydroxyapatite (HAp) sheet for adhering directly onto tooth and bone surfaces using a pulsed laser deposition method and a film isolation technique. We have investigated a method to regenerate the tooth enamel by just attaching the flexible HAp sheet onto the tooth surface with artificial saliva. In this paper, we report the result of adhering the HAp sheet to the enamel assisted by an TCP over-deposition and calcium phosphate aqueous solution in order to reduce the period required to regenerate the enamel.
  • フレキシブル極薄フッ素化アパタイトシートの作製, 本津 茂樹, 丸山 敏朗, 加藤 暢宏, 西川 博昭, 楠 正暢, 速水 尚, 橋本 典也, 日本バイオマテリアル学会大会予稿集,   2011 11
  • シリコーン製マイクロウェルの容積と形状がウシ胚の初期発生に及ぼす影響, 庄 隼生, 岩本 太作, 加藤 暢宏, 河上 泰之, 谷口 俊仁, 岸 昌生, 細井 美彦, 松本 和也, 入谷 明, 佐伯 和弘, 日本胚移植学雑誌,   2011 01
  • The restoration of dental enamel using the flexible apatite sheet, 5th International Symposium on Apatites and Correlative Biomaterials,   2010 12 , 5th International Symposium on Apatites and Correlative Biomaterials
  • Adsorption of Type I Collagen onto Poly (dimethylsiloxane) Microfluidic Device and Time-lapse Observation of HL60 Cells under Continuous Perfusion., 森本 康一, 西山 裕之, 國井 沙織, 角田 昌明, 加藤 暢宏, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要,   2010 09 01
    Summary:灌流培養環境下でHL60細胞とI型コラーゲンとの相互作用を観察するため, poly (dimethylsiloxane)を用いて微小流路デバイス(幅700μm×高さ100μm×長さ20mm)を作製した. 矩形断面を有する微小流路内壁4面すべてを酸素プラズマ処理し, ニワトリI型コラーゲンとウシ胎児血清成分タンパク質を灌流して固定化した. 固定化されたタンパク質の吸着量をSDS-ポリアクリルアミドゲル電気泳動にて定量的に調べた結果, 血清タンパク質に対して酸素プラズマ処理の効果は認められなかったが, I型コラーゲンでは酸素プラズマ処理の効果が経時的に減少するに従い, その固定化量が増加した. 作製した微小流路デバイスを用いた0.2μL/minの灌流培養下で, HL60細胞はI型コラーゲンにのみローリングあるいは接着挙動を示した. 酸素プラズマ処理の効果はI型コラーゲンに対する細胞接着に大きく影響した. HL60細胞の接着挙動は固定化されたI型コラーゲン量に単純に比例せず, その構造変化を認識することが示唆された. (英文) We developed microfluidic device (W 700μm×H 100 gμm×L 20 mm) with poly (dimethylsiloxane) to observe adhesion of HL60 cells on type I collagen under continuous perfusion. The surface of poly (dimethylsiloxane) was treated by oxygen plasma and then coated by fetal calf serum proteins or chicken type I collagen. By using SDS-polyacrylamide gel electrophoresis, we showed that treatment of oxygen plasma had no effect on binding of serum proteins on the polymer surface. However, the amount of collagen on the surface increased by decreasing of the effect of plasma treatment. On the serum protein-coated polymer surface, HL60 cells fully flowed through under 0.2μL/min flow rate. On the other hand, HL60 cells bound to the collagen-coated polymer surface and subsequently adhered to the collagen-coated polymer surface. We suggest that the conformation of collagen would alter depending on the degree of oxygen plasma treatment of PDMS and HL60 cells would recognize the difference in collagen structure.本研究は近畿大学生物理工学部戦略的研究No.06-II-1, 2006およびNo.07-IV-3, 2007の助成を受けた,
  • シリコーン製マイクロウェルプレートと用いたウシ単一胚の個別培養, 岩本 太作, 加藤 暢宏, 橋本 周, 河上 泰之, 谷口 俊仁, 庄 隼生, 岸 昌生, 細井 美彦, 松本 和也, 森本 義晴, 入谷 明, 佐伯 和弘, The Journal of Reproduction and Development,   2010 08
  • Selection of high potential embryos based on time-lapse images obtained by a combination of an individual culture in poly-(dimethylsiloxane) micro-well and a microscope inside the incubator, HASHIMOTO Shu, SAEKI Kazuhiro, KATO Nobuhiro, NAKAOKA Yoshiharu, MORIMOTO Yoshiharu, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2010 04 01
  • ポリジメチルシロキサン製マイクロウェルの容積と胚数がウシ胚の初期発生に及ぼす影響, 岩本 太作, 加藤 暢宏, 谷口 俊仁, 松井 孝徳, 高橋 千明, 中野 達也, 庄 隼生, 岸 昌生, 細井 美彦, 松本 和也, 入谷 明, 佐伯 和弘, 日本畜産学会大会講演要旨集,   2010 03
  • ポリジメチルシロキサン製マイクロウェルの容積がウシ胚の初期発生に及ぼす影響, 岩本 太作, 加藤 暢宏, 谷口 俊仁, 松井 孝徳, 高橋 千明, 中野 達也, 庄 隼生, 岸 昌生, 細井 美彦, 松本 和也, 入谷 明, 佐伯 和弘, 日本胚移植学雑誌,   2010 01
  • 無孔性PDMS製マイクロウェルプレートによるヒト体外受精卵の培養, 加藤 暢宏, 橋本 周, 森本 義晴, 佐伯 和弘, 日本バイオマテリアル学会大会予稿集,   2009 11
  • 減圧下で作製したPolydimethylsiloxane micro-wellを用いたヒト胚のtime-lapse photography, 橋本 周, 森本 義晴, 加藤 暢宏, 佐伯 和弘, 日本生殖医学会雑誌,   2009 10
  • 3C2-1 Development of a communication aid device using a touch panel for a disabled person with athetosis type cerebral paralysis, Kato Nobuhiro, Taguchi Satoshi, Joe Yoshitaka, Suzuki Eisaku, The JSME Symposium on Welfare Engineering,   2009 09 23
    Summary:A novel low-cost talking communication aid device utilizing touch panel for a disabled person with both dysphonia and athetosis type cerebral paralysis is developed.
  • PDMS製マイクロウェルプレートを用いたウシ胚の初期発生に及ぼすウェルの容積の影響, 岩本 太作, 谷口 俊仁, 松井 孝徳, 高橋 千明, 中野 達也, 庄 隼生, 岸 昌生, 加藤 暢宏, 細井 美彦, 松本 和也, 入谷 明, 佐伯 和弘, 日本畜産学会大会講演要旨集,   2009 09
  • Developmental competence of human embryos cultured individually in poly-(dimethylsiloxane) micro-well cured under reduced pressure, HASHIMOTO Shu, AKAMATSU Yoshie, IWAMOTO Daisaku, TANIGUCHI Toshihito, KATO Nobuhiro, SAEKI Kazuhiro, MORIMOTO Yoshiharu, Journal of mammalian ova research = 日本哺乳動物卵子学会誌,   2009 04 01
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ胚の体外培養の検討, 岩本 太作, 松井 孝徳, 中野 達也, 高橋 千明, 谷口 俊仁, 加藤 暢宏, 佐伯 和弘, 日本胚移植学雑誌,   2009 01
  • Poly-(Dimethylsiloxane)(PDMS) Micro Well Plates Cured Under Low Perssure for Bovine Embryo Culture, Biomedical Engineering Society,   2008 11 , Biomedical Engineering Society
  • 219 Cooperation between an Enterprise and a School in Mechanical Design Education by using 3D-CAD, FUJII Masao, KATO Nobuhiro, NAKAGAWA Hideo, SHIMIZU Takaki, SATOMI Kenichi, TAGUCHI Shinya, 関西支部講演会講演論文集,   2008 03 14
  • Rapid Prototyping of Microchannel for Braille Display Microfluidics using Printed Circuit Boards, Biomediacal Engineering Society 2007 Anuual Fall Meeting,   2007 09 , Biomediacal Engineering Society 2007 Anuual Fall Meeting
  • Collaborative development of e-Learning solution in Pro/E operation training with industry and university, PTC user world event 2005 JAPAN,   2005 11 , PTC user world event 2005 JAPAN
  • Development of e-Learning contents in 3D-CAD operation training with factory-university collaboration,   2005 09
  • Development of e-Learning contents in 3D-CAD operation training with factory-university collaboration,   2005 07
  • 212 Development of an e-Learning solution in 3D CAD operation for design class, KATO Nobuhiro, FUJII Masao, TAGUCHI Shinya, SHIMIZU Takaki, 関西支部講演会講演論文集,   2005 03 18
    Summary:This paper describes the development of an e-Learning solution in 3D CAD operation training. The e-Learning contents have following features. In operation, the contents indicate the instruction window and 3D CAD (Pro/ENGINEER[○!R]) window in same display simultaneously. The motif of the exercise models is familiar to class and enhances the motives for the exercise. The contents of the e-Learning are based on SCORM (Shareable Content Object Reference Model) standard and able to supervise the progress of the class individually. The effectiveness of the contents is confirmed by achievement check in design class exercises.
  • Development of e-Learning contents for 3D CAD operation,   2005 03
  • Polymer micro cantilevers for force controlled atomic force microscopy 3 - Improvement of fabrication process, N. Kato, C. S. Park, T. Matsumoto, H. Kikuta, K. Iwata, Proceedings of the SICE Annual Conference,   2004 12 01
    Summary:In this paper, we report on the improvements in fabrication processes of polymer micro cantilevers for force controlled atomic force microscopy. The improvement of photo mask patterns results in the enhancement of controllability of the pyramidal probe tip geometry and in rapid dissolving of sacrificial layer. It also contributes to process simplicity.
  • 223 Analysis of the complex system with greenhouse-heating and power generation by temperature difference using floating collector type solar pond, SUZUKI Takayuki, OCHI Toshiaki, KATO Nobuhiro, WATANABE Toshiaki, MATSUMOTO Toshiro, 関西支部講演会講演論文集,   2004 03 17
    Summary:Performance of complex system included power generation by temperature difference and greenhouse heating using floating collector type solar pond with two-layer was investigated for simulation models. It was also compared with salt-gradient solar pond. In thermal collector part, thermal storage layer and soil, some equations of heat transfer were drawn to establish optimum conditions for design, respectively. They were given by analysis of complex heat transfer using simulation models. They were concluded as being the following: (1) Flow rate of the water circulation on the collectors was 50(kg/(m^2・h)). (2) Minimum incident solar radiation for starting of pumping was 500(W/m^2). (3) Depth of thermal storage layer is 3〜5(m). (4) Thickness of the insulation was more than 10(cm), (5) Surface area of pond is more than 100(m^2). Although the maximum water temperature in thermal storage layer was lower than salt-gradient solar pond, annual temperature hardly changed. Consequently, this system has advantage of use during winter was shown for this system. In addition, it was clearly presented that it is possible to use also for power generation and greenhouse heating.
  • Fabrication of polymer cantilevers for force controlled atomic force microscope, Nobuhiro Kato, Choong Sik Park, Toshiro Matsumoto, Hisao Kikuta, Koichi Iwata, Proceedings of SPIE - The International Society for Optical Engineering,   2003 12 01
    Summary:We developed the fabrication process of all-plastic cantilever for force controlled atomic force microscope. The force controlled atomic force microscope can prevent damage during the scanning of the soft sample. However it has a drawback in its stability. To stabilize the system the cantilever is required to have small Q factor. The negative photo resist for MEMS (SU-8) has ideal properties, micromachinability and small Q factor, for this purpose. Here we demonstrate the cantilever consists of tip, lever and base with same polyimide. The mold of the probing tip is the pyramidal pit pattern on silicon wafer formed by direct leaser writing and anisotropic etching of silicon. Other elements of the cantilever are formed by contact lithography. The photo mask is also fabricated by direct laser writing. By arranging the dimensions of the cantilever, we can control its spring constant and resonant frequency. For the spring constant of 0.1N/m and the first resonant frequency of 2kHz, the typical dimensions of the cantilever is 1030 × 300 × 7 μm3. The fabrication error in cantilever geometry was 1.5%. The estimated resonant frequency has a good agreement with the designed value.
  • Polymer cantilevers for force controlled Atomic Force Microscope Desing and Fabrication,   2003 11
  • Fabrication of polymer cantilevers for force-controlled atomic force microscope,   2003 08
  • Education in Mechanical Design at Kinki University A case in department of Biology Oriented Science and Technology, Kato Nobuhiro, Fujii Masao, Proceedings of JSPE Semestrial Meeting,   2003 03
    Summary:学部3年生を対象に3次元CAD(Pro/Engineer ptc社)を用いたグループワークを中心とした設計製図教育を行った。CAD操作の手戻りを極力さける為に、CADそのもののスキル教育よりもグループワークによる紙上での検討に重点を置いた。その結果、学生間の意志疎通の円滑化、コミュニケーション能力の向上が見られ、協調作業による3次元モデル構築の際にその効果が発揮された。本手法による教育効果について報告する。
  • Development of diffractive beam shaping element for high power CO2 laser, 電気加工学会全国大会講演論文集,   2002 11 07
  • Education in product design in Kinki University in case of faculty of biology oriented science and technology,   2002 11
  • Cooperation between an enterprise and a school in mechanical design education by using 3D-CAD, FUJII Masao, KATO Nobuhiro, NISHIHAMA Megumi, SHIMIZU Takaki, 公開研究会・講演会技術と社会の関連を巡って : 技術史から経営戦略まで : 講演論文集,   2002 10 18
    Summary:The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. In the product design at multimedia age, it is important to promote the creative and intelligent design operation and also to construct the educational curriculum by using 3DCAD.
  • Microcantilever with High Damping Coefficent for Force Curve Messurement 2 Fabrication of Integrated Polyimide Cantilever,   2002 10
  • Stress Analysis of Cornea after Laser Ablation by FEM, IWABU Akinori, MATSUMOTO Toshiro, KATO Nobuhiro, UOZATO Hiroshi, 年次大会講演論文集 : JSME annual meeting,   2002 09 20
    Summary:Glasses and contact lenses are popular solutions for many individuals with refractive disorders. Although many techniques have been proposed and tested by the laser eye surgery, photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK) are the most widely practiced by using excimer laser. A difficult problem for all refractive surgical procedures is to predict the final refraction because the intraocular pressure (IOP) applies inside the eyes. In this paper, mechanical analysis of cornea was carried out by using finite element method (FEM) and the optical performance of these models was calculated by ray tracng.
  • Education of product design at multimedia age, FUJII Masao, KATO Nobuhiro, The proceedings of the JSME annual meeting,   2002 09
    Summary:The rapid progress of semiconductor technology promotes the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer a CAD software and so on. This paper describes the progress of mechanical design technology and the importance of the education by using 3dimensional computer aided design (3DCAD) system at multimedia age.
  • Imaging polarimetry with an micro-retarder array,   2002 08
  • Polymer Micro Cantilevers for Force Controlled Atomic Force Microscopy 2 Fabrication of Cantilevers,   2002 08
  • Cooperation between an enterprise and a school in mechanical design education by using 3D・CAD,   2002 07
  • Development of micro cantilevers made of photosensitive polyimide,   2002 01
  • Stress Analysis of Cornea after Laser Ablation by FEM, IWABU Akinori, MATSUMOTO Toshiro, KATO Nobuhiro, UOZATO Hiroshi, The proceedings of the JSME annual meeting,   2002
    Summary:Glasses and contact lenses are popular solutions for many individuals with refractive disorders. Although many techniques have been proposed and tested by the laser eye surgery, photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK) are the most widely practiced by using excimer laser. A difficult problem for all refractive surgical procedures is to predict the final refraction because the intraocular pressure (IOP) applies inside the eyes. In this paper, mechanical analysis of cornea was carried out by using finite element method (FEM) and the optical performance of these models was calculated by ray tracng.
  • Holographic deformation analysis of the human femur, T. Matsumoto, A. Kojima, N. Kato, T. Watanabe, M. Tamiwa, M. Baba, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings,   2001 12 01
    Summary:The deformations of the neck and head of the human dried femur were measured and analyzed with loads applied at the femoral head. Particular attention was paid to measurements of bones with osteoporosis. We used double exposures or real-time holographic interferometry to measure precisely. The distal part of each femur was embedded in super hard plaster, and the load was applied at the femoral head. In order to better simulate the situation of a femur under physiological muscles, we used the 3D finite element method (FEM) for our analyses. After modeling the femur, which consisted of meshing as eight noded elements for each section of diaphysis, we used the 3D FEM to calculate stress and strain. We obtained the following results : (1) the deformations increased with the progress of osteoporosis;(2) femur with osteoporosis showed obvious rotational components in the femoral shaft and (3) a compressive stress distribution could be found at the medial side of the diaphyseal region by using the FEM.
  • 311 Stress Analysis of Temporomandibular Joint applied Masticatory Force by Finite Element Method, MIYAMURA Koji, MATSUMOTO Toshiro, KATO Nobuhiro, MIYAWAKI Shoichi, KAWAKAMI Tetsuji, BABA Masato, OKAJIMA Kaoru, The Computational Mechanics Conference,   2001 11 27
  • Beam shaping for laser surface modification Study of the hologram on a curved surface, 電気加工学会全国大会講演論文集,   2001 11 08
  • Stress Analysis of Temporomandibular Joint applied Masticatory Force by Finite Element Method,   2001 11
  • CO2 laser beam shaping by using hologram on a curved surface 2 Design and evaluation,   2001 09
  • Displacement analysis of cornea after laser ablation by FEM, IWABU Akinori, MATSUMOTO Toshiro, MATSUOKA Toshihiro, UOZATO Hiroshi, KATO Nobuhiro, UEDA Yukio, 生体・生理工学シンポジウム論文集,   2001 08 29
  • Polymer micro cantilevers for force controlled atomic force microscopy,   2001 07
  • Corneal Deformation Analysis with Finite Element Method in Laser Refractive Surgery,   2001 04
  • Displacement analysis of cornea after Laser ablation by FEM, MATSUOKA Toshihiro, MATSUMOTO Toshiro, KATO Nobuhiro, UEDA Yukio, UOZATO Hiroshi, バイオエンジニアリング講演会講演論文集,   2001 01 15
  • Displacement analysis of cornea after laser ablation by,   2001 01
  • Holographic Investigation on the Deformation of the facial bone,   2000 12
  • Mechanical consideration of Temporomandibular joint by finite element stress analysis, FUJIWARA Syosuke, MATSUMOTO Toshiro, KATO Nobuhiro, UEDA Yukio, KAWAKAMI Tetsuji, BABA Masato, MIYAWAKI Shoichi, OKAJIMA Kaoru, The ... JSME Bioengineering Conference and Seminar,   2000 10 13
  • Mechanical consideration of Temporomandibular joint by finite element stress analysis, FUJIWARA Syosuke, MATSUMOTO Toshiro, KATO Nobuhiro, UEDA Yukio, KAWAKAMI Tetsuji, BABA Masato, MIYAWAKI Shoichi, OKAJIMA Kaoru, Proceedings of the JSME Bioengineering Conference and Seminar,   2000
  • 走査型近視野原子間力顕微鏡によるウシ精子の表面形状の観察, 佐伯 和弘, 加藤 暢宏, 細井 美彦, 松本 和也, 加藤 博己, 入谷 明, 日本胚移植学雑誌,   1999 01
  • On the Polymer Thickener for Viscosity-Stabilized Solar Pond, MATSUMOTO Toshiro, KATO Nobuhiro, TAGA Masao, 太陽/風力エネルギー講演論文集 = Proceedings of JSES/JWEA Joint Conference,   1998 12 05
  • A New Method for Strain Measurement by Using Multiple Color Filtering, Matsumoto Toshiro, Nagae Sadahiko, Kato Nobuhiro, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University,   1998 06
    Summary:A semi-quantitative measurement of the strain distribution by two-dimensional color dispaly is presented. The strain components are converted into color band intensity distributions in the image of the specimen. The method is based on a optical technique with the aid of the spatial filtering theory. To show a usefulness of the method, it was carried out to measure the strain distribution of a plane plate with a round hole.
  • Improvement of polymer micro cantilevers for force controlled atomic force microscope, APMF2005 Proceedings,   2005
  • Development of an e-Learning solution in 3D-CAD operation for design class, Proceedings of The 1st International Conference on Desing Engineering and Science,   2005
  • Polymer micro cantilevers for force controlled atomic force microscopy 3 --- Improvement of Fabrication Process(共著), Proceedings of SICE Annual Conference 2004 in Sapporo,   2004
  • Polymer cantilevers for force contreolled atomic force microscope - desing and fabrication -(共著), Proceedings of APMF'2003,   2003
  • Fabrication of polymer cantilevers for force controlled atomic force microscope(共著), Proceedings of SPIE,   2003
  • Imaging polarimetery with an micro-retarder array(共著), Proceedings of SICE Annual Conference 2002,   2002
  • Polymer micro cantilevers for force controlled atomic force microscopy 2 - Fabrication of cantilevers -(共著), Proceedings of SICE Annual Conference 2002 in Osaka,   2002
  • Holographic investigation on the deformation of human femur due to the load(共著), Proc. of the 12th Conf. of the European Soc. of Biomechanics,   2000
  • POLYMER MICRO CANTILEVERS FOR FORCE CONTROL ATOMIC FORCE MICROSCOPY(共著), Proceedings of the 5th Asia-Pacific Symposium on Measurement of Force, Mass and Torque,   2000
  • Deformation of cornea after ex cimer laser due to FEM(共著), Invest. Opthalmol.,   1999
  • Relation between the loads applied to the facial bone and fracture patterns by means of holographic interferometry(共著), Abstracts of the 3rd World Congress of Biomechanics,   1998
  • Mechanical analysis of cornea by means of finite element method(共著), Fifth International Conference on Optics Within Life Sciences (OWLS V),   1998
  • DYNAMIC MODEL ON FORCE FEEDBACK SYSTEM FOR NANO-NEWTON FORCE MEASUREMENT(共著), PROCEEDINGS of IMEKOTC3/APMF'98,   1998
  • Force feedback sensors for AFM (共著), Proceedings of Measurements of Force and Mass,   1996
  • Surface force detection using a force balancing method with an optical displacement sersor (共著), Proceedings of the XIII IMEKO world congress,   1994

Works

  • Improvement of 2D Leaser Direct Writting System.

Misc

  • Feasibility study to recognize cleavage of bovine early embryo based on observation image analysis by deep convolutional neural network, 63, 608, 611,   2019 05 22 , http://id.ndl.go.jp/bib/029719664
  • 椎間板組織再生のための細胞低接着性コラーゲンゲルの開発, 森本康一, 國井沙織, 武岡由樹, 由留部崇, 加藤暢宏, 尾前薫, 黒田良祐, 日本バイオマテリアル学会大会予稿集(Web), 40回, 216, 216,   2018 11 , http://jglobal.jst.go.jp/public/201802287535119865
  • 細胞低接着性コラーゲンによる神経細胞の活性化と組織再生機能の検証, 國井沙織, 兼清健志, 中野法彦, 加藤暢宏, 尾前薫, 井出千束, 森本康一, 日本バイオマテリアル学会大会予稿集(Web), 40回, 367, 367,   2018 11 , http://jglobal.jst.go.jp/public/201802276656763415
  • Fabrication of microneedles using sequential multi-wavelength exposure with moving-mask lithography, Kato Nobuhiro, Mori Shunta, Proceedings of JSPE Semestrial Meeting, 2017, 0, 333, 334,   2017 , 10.11522/pscjspe.2017S.0_333
  • Mechanical characteristic of biodegradable microneedles, Mori Shunta, Kai Takahisa, Susaki Yusuke, Yamamoto Ei, Kato Nobuhiro, Transactions of Japanese Society for Medical and Biological Engineering, 54, 27, S253, S253,   2016 , 10.11239/jsmbe.54Annual.S253
    Summary:

    Microneedles (MNs) are transdermal drug delivery systems, making it possible to administer drugs into the dermis without pain. In this study, master male molds of MNs were obtained by moving-mask UV lithography. The MNs female molds were replicated by poly-dimethylsiloxane. Finally, MNs were obtained by casting chondroitin sulfate C sodium salt (CSC) solution to the female molds. The Young's modulus of CSC was evaluated to be 2-4 GPa by tensile test. The CSC was enough stiff to penetrate epidermis. The penetration tests were conducted to characterize various shapes of CSC-MNs.

  • Analytical and experimental study of passive regulator for micro channel, Kadota Takayuki, Katou Nobuhiro, Transactions of Japanese Society for Medical and Biological Engineering, 54, 27, S255, S255,   2016 , 10.11239/jsmbe.54Annual.S255
    Summary:

    Flow rate of fluid in micro channel driven by gravity pump is proportional to the head difference. Regulator for long-term microfluidic cell culture system was needed. The conventional flow regulator systems enabling constant flow rate are not easy to miniaturize to fit on micro channels. In this study, simple passive regulator systems that can be applied to micro channel are presented. The result of FEM analysis predicted the effectiveness of deformable valve structure. The analyzed results were evaluated by experiment using microfluidic system with 4 types deformable passive valves.

  • 開発したタイムラプスモニタリング装置によるウシ胚体外培養の観察, 藤木雄太, 加藤暢宏, 宮田静, 朴忠植, 山東悠介, 佐伯和弘, 日本はい移植学雑誌, 38, 1, 43, 43,   2016 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201602216525391140
  • P-40 Evaluation on the effect of lap-strake perforated amorphous calcium phosphate sheets on the sealing of dentinal tubules, Ido Y, Isai A, Yamamoto E, Kato N, Nishikawa H, Yasuo K, Hashimoto Y, Yoshikawa K, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices, 34, 2, 111, 111,   2015 03
    Summary:We propose a new method to shield dentinal tubules by direct attaching an amorphous calcium phosphate (ACP) sheet on the dentin as a treatment of the hyperaesthesia. In this study, we fabricated the perforated ACP sheet in order not only to easily remove liquid on the dentinal surface but also to promote the remineralization at the interface between the sheet and dentin. The ACP sheet was prepared by the pulsed laser deposition technique and the laser process using a metal mask. The interface structure between the ACP sheet and dentin was observed by a SEM. In addition, using Pashle's method, we evaluated the inhibition effect of lap-strake perforated ACP sheets attached to the dentin on its permeability.
  • Formation of self degradable microneedle array using moving mask lithography, KAI TAKAHISA, MORI SHUNTA, KATO NOBUHIRO, Transactions of Japanese Society for Medical and Biological Engineering, 53, 0, S186_01, S186_01,   2015 , 10.11239/jsmbe.53.S186_01
    Summary:A moving-mask exposure apparatus that allows backside exposure through transparent substrate to form the mold of microneedle patch with thick photoresist was developed. By choosing moving trajectory, various shape microneedle can be produced from a single mask pattern. The photoresist microneedle pattern was replicated by poly-dimethylsiloxane (PDMS). The mixture of chondroitin sulfate C and sterilized water was poured over the PDMS mold. Finally, the cured chondroitin sulfate C microneedle patch was detached from the PDMS mold. From scanning microscope observation, successful shape transfer form the photoresist MN to the chondroitin sulfate C microneedle was confirmed.
  • GnRH‐FSH処理が黒毛和種雌牛のOPUに及ぼす影響, TANIGUCHI SHUNJI, TAKADA HIROTATSU, TARUMOTO HIDEYUKI, KATO NOBUHIRO, SAEKI KAZUHIRO, 日本はい移植学雑誌, 37, 1, 38, 38,   2015 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502256790927234
  • Development of embryo monitoring system using time lapse cinematography, MIYATA SHIZUKA, PARK CHOONGSIK, SANDO YUSUKE, SAEKI KAZUHIRO, KATO NOBUHIRO, Transactions of Japanese Society for Medical and Biological Engineering, 53, 0, S228_03, S228_03,   2015 , 10.11239/jsmbe.53.S228_03, http://ci.nii.ac.jp/naid/130005163989
    Summary:A simple time-lapse cinematography device that provides useful insight for embryo quality scoring by series observation of embryos during culture was developed. The device consists of a microscope system, a PC controlled camera and an illumination system. All components are implemented in a waterproof enclosure to be operated inside a CO2 incubator. Operations for image capture including focus adjustment and illumination adjustment can be remotely performed using PC without removing embryos from the optimal gas and temperature conditions. Using this device, clear images including several embryos were captured and the cleavage timing of embryos can be exactly identified.
  • B203 Migration property of fibroblast cultured on an elastin-coating substrate, NISHI Shingo, SUSAKI Yusuke, KAI Takahisa, MIYATA Shizuka, NISHIMOTO Masaya, TAKEMORI Kumiko, KATO Nobuhiro, ITO Hiroyuki, YAMAMOTO Ei, The Proceedings of the JSME Conference on Frontiers in Bioengineering, 2015, 0, 107, 108,   2015 , 10.1299/jsmebiofro.2015.26.107, http://ci.nii.ac.jp/naid/110010051941
  • P-15 Evaluation of the dentin permeability inhibition effect by lap-strake method of the amorphous calcium phosphate sheet, Isai A, Yamamoto E, Kato N, Nishikawa H, Yasuo K, Hashimoto Y, Yoshikawa K, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices, 33, 5, 401, 401,   2014 09
    Summary:We have fabricated a flexible hydroxyapatite (HAp) sheet using pulsed laser deposition and a sheet isolation technique. We aim to form an artificial enamel layer on the dentin by an amorphous calcium phosphate (ACP) sheet with solubility higher than compared that of the crystallized sheet. In this study, using Pashle's method, we evaluated of the dentin permeability inhibition effect by double layer method of the ACP sheet on dentin as a cure for dental hypersensitivity. The dentin permeability inhibition rate of the dentinal tubule by double layer method of the ACP sheet was 89.17±7.24%. The ACP sheet was yielded results better than of dental resin cement.
  • P-16 Preparation and evaluation of ultra thin potassium-doped hydroxyapatite sheet, Hatoko Y, Yamamoto E, Kato N, Nishikawa H, Yoshikawa K, Furuzono T, Yamamoto K, Hontsu S, The journal of the Japanese Society for Dental Materials and Devices, 33, 5, 402, 402,   2014 09
    Summary:In toothpaste formulated for hypersensitivity, potassium compounds such as potassium nitrate and potassium bromide are often added because it is believed that neurotransmission is blocked by the function of potassium ions. Thus far, we have proposed attaching a hydroxyapatite sheet on dentin as a treatment for hyperesthesia. We think that it may be possible to block dental tubules and relieve pain by adding potassium to the sheet for sustained release. In this study, we prepared and evaluated a potassium-doped hydroxyapatite thin film, and applied the process to the preparation of a potassium-doped hydroxyapatite sheet.
  • A-18 Examination of dentine restoration using the Er:YAG laser ablation method (Part1), Hontsu S, Yamamoto E, Kato N, Nishikawa H, Hashimoto Y, Yoshikawa K, Yamamoto K, The journal of the Japanese Society for Dental Materials and Devices, 33, 2, 128, 128,   2014 03
    Summary:A calcium phosphate film was deposited on the dentine in the air by applying Er:YAG laser to the laser ablation (PLD) method to try the restoration of the dentine. α-tricalcium phosphate (α-TCP) was used as a target for the film preparation, and cattle enamel and human dentin were used as a substrate. X-ray diffraction (XRD) technique was used for crystallinity evaluation of the deposited layer, and scanning electron microscope (SEM) was used for the evaluation of the substrate-deposited layer interface.
  • 21pm3-PM003 Microneedle pattern formation by using moving-mask lithography, Kai Takahisa, Hirano Masakazu, Kato Nobuhiro, The Proceedings of the Symposium on Micro-Nano Science and Technology, 2014, 0, _21pm3, PM0-_21pm3-PM0,   2014 , 10.1299/jsmemnm.2014.6._21pm3-PM0_3
    Summary:Microneedle patch is a minimally invasive drug delivery system for dermal vaccination. The shape of the needle is restricted by manufacturing method. Here, we propose a moving-mask UV lithography with backside exposure to form the mold of microneedle patch. In this method, the photo mask with circle opening was fixed, and resist-coated glass substrate was moved in circular motion during exposure. Altering diameter of circular trajectory of motion can produce arbitrary UV light intensity distribution which results in the corn shape of negative tone photoresist. We obtained the microneedle measured 100μm basal diameter and 450μm tall with sharp tips tapering to a 10μm radius of curvature.
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養, 岩本 太作, 谷口 俊仁, 岸 昌生, 加藤 暢宏, 佐伯 和弘, 日本胚移植学雑誌, 36, 1, 54, 54,   2014 01
  • B112 Fibroblast culture using an elastin-coated dish, NISHI Shingo, SUSAKI Yusuke, MIYATA Shizuka, TAKEDA Ippei, TAKEMORI Kumiko, KATO Nobuhiro, ITO Hiroyuki, YAMAMOTO Ei, The Proceedings of the JSME Conference on Frontiers in Bioengineering, 2014, 0, 51, 52,   2014 , 10.1299/jsmebiofro.2014.25.51, http://ci.nii.ac.jp/naid/110009964986
  • Application of Ultrathin Amorphous Calcium Phosphate Sheet in the Curative Treatment of Dentin Hypersensitivity in vitro, S. Hontsu, A. Isai, E. Yamamoto, N. Kato, K. Yoshikawa, H. Nishikawa, M. Kusunoki, K. Yamamoto, 13th Asian BioCeramics Symposium,   2013 12
  • J112012 Generation of 2.5 dimensional microstructure by moving mask UV exposure, KATO Nobuhiro, SHARMA Markun, MAESAKA Keisuke, HIRANO Masakazu, The Proceedings of Mechanical Engineering Congress, Japan, 2013, 0, _J112012, 1-_J112012-3,   2013 , 10.1299/jsmemecj.2013._J112012-1
    Summary:A novel moving mask exposure apparatus for 2.5 dimensional patterning of thick photo resist has been developed. In general mask aligner, the UV light intensity that passes through photo mask shows exactly same shape of mask pattern. By in plane moving PZT-stage which carrying photoresist coated substrate, the UV intensity distribution via photo mask has been modified from simple binary pattern to Gaussian like pattern. The stage movement can be controlled by applying signal to stage that enables to generate arbitrary UV intensity distribution. Using the exposure apparatus and backside exposure technique we created photoresist molds for micro well array that have smoothly tilted sidewall. These photo resist molds were replicated by Poly-(dimethylpolysiloxane) to make final product with good shape transferability.
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ卵子の成熟培養, IWAMOTO DAISAKU, TANIGUCHI SHUNJI, KISHI MASAO, KATO NOBUHIRO, SAEKI KAZUHIRO, 東日本家畜受精卵移植技術研究会大会資料, 29th, 65,   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302284283206567
  • Formation of the artificial enamel on dentin using the ultrathin apatite sheet, N. Kato, K. Yoshikawa, A. Isai, E. Yamamoto, H. Nishikawa, M. Kusunoki, Y. Hashimoto, K. Yamamoto, S. Hontsu, The 12th Asian BioCeramics Symposium,   2012 11
  • Development of a perforated hydroxyapatite sheet and its application to the repair of tooth enamel, E. Yamamoto, A. Isai, N. Kato, H. Nishikawa, M. Kusunoki, T. Hayami, K. Yoshikawa, K. Yamamoto, S. Hontsu, The 12th Asian BioCeramics Symposium,   2012 11
  • ムービングマスク露光法によるグレースケールリソグラフィ, KATO NOBUHIRO, MISHINA TAKAHIRO, SAEKI KAZUHIRO, 化学とマイクロ・ナノシステム研究会講演要旨集, 25th, 67,   2012 05 17 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202267821675492
  • Examination of enamel regeneration method using the hydroxyapatite/α-TCP composite sheet, Hontsu Shigeki, Yoshikawa Kazushi, Matsuda Taiyo, Kusunoki Masanobu, Maruyama Toshiro, Yamamoto Ei, Kato Nobuhiro, Nishikawa Hiroaki, Preprints of Annual Meeting of The Ceramic Society of Japan
    Preprints of Fall Meeting of The Ceramic Society of Japan, 2012, 0, 706, 706,   2012 , 10.14853/pcersj.2012S.0.706.0
    Summary:We have succeeded to regenerate tooth enamel by using flexible hydroxyapatite (HAp) sheets. In this study, we tried enamel regeneration by composite sheet which deposited a α-TCP thin film on the HAp sheet. The HAp/α-TCP composite sheets are stick onto polished surface of extracted teeth with calcium phosphate solution. The time dependence of the composition at the interfaces between the composite sheet and the teeth surfaces was evaluated by an X-ray diffraction (XRD) method. After one day past, the XRD pattern completely matched with that of teeth enamel. Also, the adherence between composite sheet and teeth enamel was evaluated by tensile test.
  • K11300 Education of Design from Scratch, Matsubara Atsushi, Kogiso Nozomu, Kato Nobuhiro, Yokokoji Yasuyoshi, Tutsui Shinsaku, Makino Osamu, The Proceedings of Mechanical Engineering Congress, Japan, 2012, 0, _K11300, 1_-_K11300-4_,   2012 , 10.1299/jsmemecj.2012._K11300-1_
    Summary:An education method for mechanical design is discussed. In conventional design education, the prototype designs together with design process are given for students, which can be referred to as parameterized design. This is a good introduction for students to start the design class. However, the big picture of the design process could not be learned. For students to experience total design paradigm, a design seminar has been opened every summer in past decade. We discuss the pros and cons of the current seminar and present the perspective of next turn.
  • Regeneration of enamel by a self-organization process of apatite sheet, Hontsu Shigeki, Yamamoto Kazuyo, Kato Nobuhiro, Yamamoto Ei, Maruyama Toshiro, Matsuda Taiyo, Nisikawa Hiroaki, Kusunoki Masanobu, Hayami Takashi, Yoshikawa Kazushi, Preprints of Annual Meeting of The Ceramic Society of Japan
    Preprints of Fall Meeting of The Ceramic Society of Japan, 2011, 0, 231, 231,   2011 , 10.14853/pcersj.2011F.0.231.0
    Summary:We have attempted to regenerate tooth enamel artificially by using flexible hydroxyapatite (HAp) sheets. The sheets are affixed onto polished surface of extracted teeth with calcium phosphate solution. The time-dependent variation of the composition at the interfaces between the HAp sheets and the teeth surfaces was evaluated by an X-ray diffraction (XRD) method. After one week past, the XRD pattern completely matched with that of teeth enamel. The adherence between HAp sheet and teeth enamel was evaluated by tensile test using a stainless rod with a diameter of 3 mm glued onto the HAp sheet. The test validated that the adherence between HAp sheet and tooth enamel is much stronger than 3.4 MPa in affixing post-21 days.
  • シリコーン製マイクロウェルの容積と形状がウシ胚の初期発生に及ぼす影響, SHO HAYAO, IWAMOTO DAISAKU, KATO NOBUHIRO, KAWAKAMI YASUYUKI, TANIGUCHI SHUNJI, KISHI MASAO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 33, 1, 46, 46,   2011 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102204903690989
  • シリコーン製マイクロウェルプレートと用いたウシ単一胚の個別培養, IWAMOTO DAISAKU, KATO NOBUHIRO, HASHIMOTO SHU, KAWAKAMI YASUYUKI, TANIGUCHI SHUNJI, SHO HAYAO, KISHI MASAO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MORIMOTO YOSHIHARU, IRITANI AKIRA, SAEKI KAZUHIRO, J Reprod Dev, 56, Suppl., j107, j107,   2010 08 , 10.14882/jrds.103.0.97.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002228276349370
  • 超音波加工によるマイクロウェルプレートの作成と応用, KATO NOBUHIRO, SAEKI DAISUKE, KONO SHINJI, IWAMOTO DAISAKU, SAEKI KAZUHIRO, 化学とマイクロ・ナノシステム研究会講演要旨集, 21st, 89,   2010 06 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002255807507649
  • Selection of high potential embryos based on time-lapse images obtained by a combination of an individual culture in poly-(dimethylsiloxane) micro-well and a microscope inside the incubator, HASHIMOTO Shu, SAEKI Kazuhiro, KATO Nobuhiro, NAKAOKA Yoshiharu, MORIMOTO Yoshiharu, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 27, 2, S27, S27,   2010 04 , http://ci.nii.ac.jp/naid/10026350702
  • ポリジメチルシロキサン製マイクロウェルの容積と胚数がウシ胚の初期発生に及ぼす影響, IWAMOTO DAISAKU, KATO NOBUHIRO, TANIGUCHI SHUNJI, MATSUI TAKANORI, TAKAHASHI CHIAKI, NAKANO TATSUYA, SHO HAYAO, KISHI MASAO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, IRITANI AKIRA, SAEKI KAZUHIRO, 日本畜産学会大会講演要旨, 112回, 88, 88,   2010 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002234121233928
  • ポリジメチルシロキサン製マイクロウェルを用いて作製したウシ体外受精胚の移植による受胎, TANIGUCHI SHUNJI, FUKUHARA JUNKO, IWAMOTO DAISAKU, KATO NOBUHIRO, SAEKI KAZUHIRO, 日本獣医師会三学会年次大会講演要旨集, 2009, 107,   2010 01 07 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502297153728544
  • ポリジメチルシロキサン製マイクロウェルの容積がウシ胚の初期発生に及ぼす影響, IWAMOTO DAISAKU, KATO NOBUHIRO, TANIGUCHI SHUNJI, MATSUI TAKANORI, TAKAHASHI CHIAKI, NAKANO TATSUYA, SHO HAYAO, KISHI MASAO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 32, 1, 52, 52,   2010 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002265202426162
  • 減圧下で形成したPDMS製マイクロウェルプレートによるウシ胚の体外培養, KATO NOBUHIRO, IWAMOTO DAISAKU, TANIGUCHI SHUNJI, SAEKI KAZUHIRO, 化学とマイクロ・ナノシステム研究会講演要旨集, 20th, 120,   2009 11 07 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002206006564712
  • 無孔性PDMS製マイクロウェルプレートによるヒト体外受精卵の培養, 加藤 暢宏, 橋本 周, 森本 義晴, 佐伯 和弘, 日本バイオマテリアル学会大会予稿集, 31回, 200, 200,   2009 11
  • 減圧下で作製したPolydimethylsiloxane micro‐wellを用いたヒト胚のtime‐lapse photography, HASHIMOTO MAKOTO, MORIMOTO YOSHIHARU, KATO NOBUHIRO, SAEKI KAZUHIRO, 日本生殖医学会雑誌, 54, 4, 311, 311,   2009 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902278173829000
  • PDMS製マイクロウェルプレートを用いたウシ胚の初期発生に及ぼすウェルの容積の影響, IWAMOTO DAISAKU, TANIGUCHI SHUNJI, MATSUI TAKANORI, TAKAHASHI CHIAKI, NAKANO TATSUYA, SHO HAYAO, KISHI MASAO, KATO NOBUHIRO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, IRITANI AKIRA, SAEKI KAZUHIRO, 日本畜産学会大会講演要旨, 111回, 80, 80,   2009 09 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902267399858079
  • Developmental competence of human embryos cultured individually in poly-(dimethylsiloxane) micro-well cured under reduced pressure, HASHIMOTO Shu, AKAMATSU Yoshie, IWAMOTO Daisaku, TANIGUCHI Toshihito, KATO Nobuhiro, SAEKI Kazuhiro, MORIMOTO Yoshiharu, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 26, 2, S25,   2009 04 01 , http://ci.nii.ac.jp/naid/10024964901
  • ポリジメチルシロキサン製マイクロウェルを用いたウシ胚の体外培養の検討, IWAMOTO DAISAKU, MATSUI TAKANORI, NAKANO TATSUYA, TAKAHASHI CHIAKI, TANIGUCHI SHUNJI, KATO NOBUHIRO, SAEKI KAZUHIRO, 日本はい移植学雑誌, 31, 1, 55, 55,   2009 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902274090702879
  • 3C2-1 Development of a communication aid device using a touch panel for a disabled person with athetosis type cerebral paralysis, Kato Nobuhiro, Taguchi Satoshi, Joe Yoshitaka, Suzuki Eisaku, The Proceedings of the JSME Symposium on Welfare Engineering, 2009, 0, 213, 214,   2009 , 10.1299/jsmewes.2009.213
    Summary:A novel low-cost talking communication aid device utilizing touch panel for a disabled person with both dysphonia and athetosis type cerebral paralysis is developed.
  • 219 Cooperation between an Enterprise and a School in Mechanical Design Education by using 3D-CAD, FUJII Masao, KATO Nobuhiro, NAKAGAWA Hideo, SHIMIZU Takaki, SATOMI Kenichi, TAGUCHI Shinya, The Proceedings of Conference of Kansai Branch, 2008, 0, _2, 27_,   2008 , 10.1299/jsmekansai.2008.83._2-27_
  • Pro/ENGINEER for biginners,   2005 05
  • 212 Development of an e-Learning solution in 3D CAD operation for design class, KATO Nobuhiro, FUJII Masao, TAGUCHI Shinya, SHIMIZU Takaki, The Proceedings of Conference of Kansai Branch, 2005, 0, _2, 27_-_2-28_,   2005 , 10.1299/jsmekansai.2005.80._2-27_, http://ci.nii.ac.jp/naid/110006187336
    Summary:This paper describes the development of an e-Learning solution in 3D CAD operation training. The e-Learning contents have following features. In operation, the contents indicate the instruction window and 3D CAD (Pro/ENGINEER[○!R]) window in same display simultaneously. The motif of the exercise models is familiar to class and enhances the motives for the exercise. The contents of the e-Learning are based on SCORM (Shareable Content Object Reference Model) standard and able to supervise the progress of the class individually. The effectiveness of the contents is confirmed by achievement check in design class exercises.
  • ABC for Pro/ENGINEER,   2004 11
  • 223 Analysis of the complex system with greenhouse-heating and power generation by temperature difference using floating collector type solar pond, SUZUKI Takayuki, OCHI Toshiaki, KATO Nobuhiro, WATANABE Toshiaki, MATSUMOTO Toshiro, The Proceedings of Conference of Kansai Branch, 2004, 0, _2, 47_-_2-48_,   2004 , 10.1299/jsmekansai.2004.79._2-47_
    Summary:Performance of complex system included power generation by temperature difference and greenhouse heating using floating collector type solar pond with two-layer was investigated for simulation models. It was also compared with salt-gradient solar pond. In thermal collector part, thermal storage layer and soil, some equations of heat transfer were drawn to establish optimum conditions for design, respectively. They were given by analysis of complex heat transfer using simulation models. They were concluded as being the following: (1) Flow rate of the water circulation on the collectors was 50(kg/(m^2・h)). (2) Minimum incident solar radiation for starting of pumping was 500(W/m^2). (3) Depth of thermal storage layer is 3〜5(m). (4) Thickness of the insulation was more than 10(cm), (5) Surface area of pond is more than 100(m^2). Although the maximum water temperature in thermal storage layer was lower than salt-gradient solar pond, annual temperature hardly changed. Consequently, this system has advantage of use during winter was shown for this system. In addition, it was clearly presented that it is possible to use also for power generation and greenhouse heating.
  • Education in Mechanical Design at Kinki University - A Case in Department of Biology Oriented Science and Technology, Kato Nobuhiro, Fujii Masao, Proceedings of JSPE Semestrial Meeting, 2003, 0, 665, 665,   2003 , 10.11522/pscjspe.2003S.0.665.0
  • Improvement of 2D laser beam pattern generator (Application to 3D suface drawing), 170, 173,   2002 03
  • CO2 Laser beam shaping with a multilevel CGH, Proceedings of JSPE Semestrial Meeting, 2002, 0, 515, 515,   2002 , 10.11522/pscjspe.2002A.0.515.0
  • Cooperation between an enterprise and a school in mechanical design education by using 3D-CAD, FUJII Masao, KATO Nobuhiro, NISHIHAMA Megumi, SHIMIZU Takaki, The Proceedings of the Tecnology and Society Conference, 2002, 0, 11, 14,   2002 , 10.1299/jsmetsd.2002.11
    Summary:The rapid progress of semiconductor technology has been promoting the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer, a CAD software and so on. In the product design at multimedia age, it is important to promote the creative and intelligent design operation and also to construct the educational curriculum by using 3DCAD.
  • Stress Analysis of Cornea after Laser Ablation by FEM, IWABU Akinori, MATSUMOTO Toshiro, KATO Nobuhiro, UOZATO Hiroshi, The proceedings of the JSME annual meeting, 2002, 0, 155, 156,   2002 , 10.1299/jsmemecjo.2002.1.0_155
    Summary:Glasses and contact lenses are popular solutions for many individuals with refractive disorders. Although many techniques have been proposed and tested by the laser eye surgery, photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK) are the most widely practiced by using excimer laser. A difficult problem for all refractive surgical procedures is to predict the final refraction because the intraocular pressure (IOP) applies inside the eyes. In this paper, mechanical analysis of cornea was carried out by using finite element method (FEM) and the optical performance of these models was calculated by ray tracng.
  • Education of product design at multimedia age, FUJII Masao, KATO Nobuhiro, The proceedings of the JSME annual meeting, 2002, 0, 391, 392,   2002 , 10.1299/jsmemecjo.2002.1.0_391
    Summary:The rapid progress of semiconductor technology promotes the digitalization of a product, and also the digitalization of infrastructure of product design by using a computer a CAD software and so on. This paper describes the progress of mechanical design technology and the importance of the education by using 3dimensional computer aided design (3DCAD) system at multimedia age.
  • Displacement analysis of cornea after Laser ablation by FEM, IWABU Akinori, MATSUMOTO Toshiro, MATSUOKA Toshihiro, UOZATO Hiroshi, KATO Nobuhiro, UEDA Yukio, 16, 103, 106,   2001 08 29
  • Displacement analysis of cornea after Laser ablation by FEM, MATSUOKA Toshihiro, MATSUMOTO Toshiro, KATO Nobuhiro, UEDA Yukio, UOZATO Hiroshi, The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME, 2001, 0, 48, 49,   2001 , 10.1299/jsmebio.2001.13.48
  • 311 Stress Analysis of Temporomandibular Joint applied Masticatory Force by Finite Element Method, MIYAMURA Koji, MATSUMOTO Toshiro, KATO Nobuhiro, MIYAWAKI Shoichi, KAWAKAMI Tetsuji, BABA Masato, OKAJIMA Kaoru, The Proceedings of The Computational Mechanics Conference, 2001, 0, 283, 284,   2001 , 10.1299/jsmecmd.2001.14.283
  • Mechanical consideration of Temporomandibular joint by finite element stress analysis, FUJIWARA Syosuke, MATSUMOTO Toshiro, KATO Nobuhiro, UEDA Yukio, KAWAKAMI Tetsuji, BABA Masato, MIYAWAKI Shoichi, OKAJIMA Kaoru, Proceedings of the JSME Bioengineering Conference and Seminar, 2000, 0, 157, 158,   2000 , 10.1299/jsmebs.2000.11.0_157
  • Control of Surface Recombination of Si Wafers by an External Electrode, ICHIMURA Masaya, HIRANO Masashi, KATO Naoki, ARAI Eisuke, TAKAMATSU Hiroyuki, SUMIE Shingo, Japanese Journal of Applied Physics, 38, 3, 292, 294,   1999 03 01 , 10.1143/jjap.38.L292, http://id.ndl.go.jp/bib/4711069
    Summary:A transparent electrode was placed close to a Si wafer, and a negative or positive voltage up to 2 kV was applied to the electrode with respect to the wafer. The wafer was irradiated through the electrode with a pulsed laser, and the decay of the excess carriers was monitored by the microwave reflectance photoconductivity decay (μ -PCD) method. The carrier lifetime of p- and n-type as-polished wafers was increased by a factor of 3 by application of a 2 kV negative voltage to the electrode. This showed that the surface recombination velocity was controlled by the external electrode. A different tendency was observed for the oxidized wafers, and these results were explained by considering the surface charge and the surface state.
  • On the Polymer Thickener for Viscosity-Stabilized Solar Pond, MATSUMOTO Toshiro, KATO Nobuhiro, TAGA Masao, 1998, 353, 356,   1998 12 05
  • Oxygen Plasma Treatment of Rubber Surface by the Atmospheric Pressure Cold Plasma Torch, LEE Bong-ju, KUSANO Yukihiro, KATO Nobuko, NAITO Kazuo, HORIUCHI Takao, KOINUMA Hideomi, Japanese Journal of Applied Physics, 36, 5, 2888, 2891,   1997 05 01 , 10.1143/JJAP.36.2888, http://id.ndl.go.jp/bib/4219761
    Summary:A new application of the atmospheric cold plasma torch has been investigated. Namely, the surface treatment of an air-exposed vulcanized rubber compound. The effect of plasma treatment was evaluated by the bondability of the treated rubber compound with another rubber compound using a polyurethane adhesive. The adhesion property was improved by treatment of the rubber compound with plasma containing oxygen radicals. Physical and chemical changes of the rubber surface as a result of the plasma treatment were analyzed by field emission scanning electron microscopy (FE-SEM) and fourier transform infrared attenuated total reflection (FTIR-ATR).
  • Fabrication of Fluoridated Hydroxyapatite Thin Film and Sheet, S. Hontsu, N. Kato, H. Nishikawa, M. Kusunoki, T. Hayami, Y. Hashimoto, The 11th Asian BioCeramics Symposium in conjunction with the 22nd Symposium on Apatite, 1, 2
  • Selection of high potential embryos through culture in poly-(dimethylsiloxane) micro-wells and time-lapse imaging, S. Hashimoto, N. Kato, K. Saeki, Y. Morimoto, HUMAN REPRODUCTION, 25, I191, I191,   2010 06

Awards & Honors

  •   2019 11 , The First Asian Pacific Division of the World Federation for Laser Dentistry Conference 2019, Award of Excellence Poster, Occulusion of dental tubule with amorphous calcium phosphate sheet prepared by KrF pulsed laser deposition
  •   2005 , JSME Educational Award

Research Grants & Projects

  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Development of automated system of in-vitro production of bovine embryos, We produced PDMS micro-wells connected with narrow canals. Bovine oocytes were introduced into the wells and medium was changed through the canals, but oocytes were trapped into the canals. Therefore, we developed simple bovine IVP systems. Bovine oocytes were introduced into test tubes filled with maturation medium. The oocytes were matured then only medium was changed by small pipets with IVF medium. The oocytes were fertilized, then medium was then changed with development medium. The IVF oocytes were cultured for 168 hours.The blastocyst rates were the same with the control method. We also examine the cleavage timing of bovine IVF oocytes with small TLC device which we originally produced. We can predict blastocyst development by examining the cleavage timing.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Force controlled atomic force microscopy for biological specimen., Feasibility of polyimide cantilever for force feedback method is studied. The stability of the force feedback system is mainly improved by small Q factor and the low resonant frequency of the cantilever. The polyimide cantilever is fabricated and investigated its mechanical feature, especially Q factor in a vacuum. Form these values the required bandwidth of the force feedback system is estimated. The polyimide cantilever reduces the frequency requirement to 1/20, compared with the SiN cantilever with same feature except for internal damping. The system stability is confirmed by numerical simulations. The bandwidth shortage results in large oscillation of the force curve. To increase biological compatibility of Ti-6Al-4V, thin film of hydroxyapatite (HAp) was deposited on the polished substrate(0.1〜3.0μmR_) by ArF excimer laser in an atmosphere of O_2+H_2O.The gas pressure was kept at 13.3Pa (1×10^<-1>Torr) during the deposition. The substrate temperature was varied torn 500℃ to 560℃. Crystal stractures of HAp film were evaluated by X-ray diffraction (XRD). At temperatures less than 500℃ the films resulted in amorphous. At temperatures greater than 560℃ the films had mixture phase of crystallized HAp and tricalcium phosphate (TCP). Surface morphology of HAp film was observed by atomic-force microscope (AFM). Mechanical properties of film were measured by the scratch test and the peeling off test by using tensile test machine. The following results were obtained : (1) The crystallized HAp was obtained at about 530℃, (2) In the scratch test, HAp film was clearly removed at the load of 18N, (3) In the peeling off test, the adhesive strength of HAp film on the substratewere about 2.3, 5.4 and 10MPa for the surface roughness 0.1, 1.0 and 3.0μmRmax, respectively and (4) Biocompatibility of HAp film was evaluated by in-vitro. Unfortunately, to investigate biological specimen the stability of the force control atomic force microscopy system is not sufficient. Farther improvement of the force control system is required.
  • Study on Force-balancing Type Atomic Force Microscope