KINDAI UNIVERSITY


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AKITA Motomu

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FacultyDepartment of Biotechnological Science / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
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Commentator Guidehttps://www.kindai.ac.jp/meikan/881-akita-motomu.html
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Last Updated :2020/09/02

Education and Career

Education

  •  - 1983 , University of Tsukuba
  •  - 1983 , University of Tsukuba

Academic & Professional Experience

  •   2010 , professor, Dept Biotech. Sci., Kinki Univ.

Research Activities

Research Areas

  • Life sciences, Applied molecular and cellular biology
  • Environmental science/Agricultural science, Agricultural environmental and information engineering
  • Environmental science/Agricultural science, Agricultural environmental and information engineering
  • Life sciences, Applied biochemistry

Research Interests

  • Applied Plant Molecular Biology, Plant Tissue Culture, Applied Microbiochemistry and Applied Biochemistry

Published Papers

  • A simple method for making CO2 enriched micro-environment by a fluorinated (FEP) film and its application, Motomu Akita, Satoki Kurimoto, Yuusuke Kominami, Memoires of Institute of Advanced Technology, Kindai University, Memoires of Institute of Advanced Technology, Kindai University, (25), 11 - 16, Mar. 2020 , Refereed
  • Sunagoke Moss (Racomitrium japonicum) Used for Greening Roofs Is Severely Damaged by Sclerotium delphinii and Protected by a Putative Bacillus amyloliquefaciens Isolate., Tamura M, Tanabe M, Valkonen JPT, Akita M, Front. Microbiol., Front. Microbiol., 10, 372, Feb. 2019 , Refereed
  • A Study on Kiishimotsuke, an Indigenous Plant to Wakayama Prefecture : Adaptation to Serpentine Soil and Molecular Phylogenetic Comparison with Iwashimotsuke and Tosashimotsuke, 明渡 絵里朱, 平田 智子, 上井 和幸, 髙木 祐子, 水野 隆文, 水野 直治, 小林 真, 小池 孝良, 大和 勝幸, 秋田 求, 泉井 桂, Memoirs of Institute of Advanced Technology, Kindai University, Memoirs of Institute of Advanced Technology, Kindai University, (21), 33 - 48, May 2016 , Refereed
    Summary:バラ科シモツケ属のキイシモツケ(Spiraea nipponica Maxim. var. ogawae( Nakai) Yamanaka)は、蛇紋岩地帯にて自生する和歌山県固有の植物である。県北部に位置する龍門山の山頂付近の群落は県の天然記念物に指定されている。キイシモツケは、かつては新種とされSpiraea ogawai Nakai と命名され登録されていた。しかし近年は形態学的比較に基づいて、高知県に自生するトサシモツケ(Spiraea nipponica Maxim. var. tosaensis( Yatabe) Makino)と共に、近畿以北に自生するイワシモツケ(Spiraea nipponica Maxim.)の変種とされ、学名も上記のように改められている。本研究では、キイシモツケがトサシモツケおよびイワシモツケなどの近縁種とは顕著に異なるのかどうかを初めて分子系統分類学の立場から検討した。同時に、キイシモツケの蛇紋岩土壌との関わりについて若干の検討を行った。まず、3 種類それぞれに特徴的な葉の形態は、種子から同一の環境条件下で生育させても再現され、生来の形質であることがわかった。次に、龍門山の土壌の交換性金属イオンの元素組成を調べ、蛇紋岩土壌特有の組成をもつことが確かめられた。キイシモツケの植物体について、金属イオンの元素分析を行った結果、蛇紋岩土壌の元素の中で最も植物に対する毒性が強いとされるニッケル(Ni)が植物体内に取り込まれていることを認めた。さらにキイシモツケの種子は発芽時に3 者の中で最も強いNi 耐性を示し、若い苗では10 mmol L - 1 のNi 2 + に対しても耐性を示した。葉緑体DNA のtRNA のtrnL – trnF 領域および核ゲノムDNA の5S RNA 遺伝子とその両端のスペーサー配列を含むITS 領域の塩基配列を解析した。その結果、葉緑体のtRNA 領域(約1000 塩基対)は完全に一致し、ITS 領域(約640 塩基対)では3 種は互いに数塩基(1%以下)異なるのみであり、分子系統学的比較においてもキイシモツケはイワシモツケおよびトサシモツケと極めて近縁であることが証明された。したがって、キイシモツケは日本の近畿以北に広く分布するイワシモツケを起源とし、和歌山の限られた蛇紋岩地帯に適応して、地理的に隔絶して自生するにいたったエコタイプであると推測された。A plant called Kiishimotsuke( in Japanese) is indigenous to Wakayama prefecture in Japan. The plant vegetates only in the three narrow mountain areas consisting of serpentine soils. The largest colony of this plant in the area of Mt. Ryumon higher than 600 m above sea level had been designated as a prefectural natural treasure since May 1971. The plant was first discovered by Y. Ogawa, a botanist in Iwade city, and registered as a new species with a name of Spiraea ogawai Nakai in 1928. However, the validity of this registration was questioned later, because there were two kinds of plants, called Iwashimotsuke and Tosashimotsuke, which were morphologically similar to Kiishimotuke except for the shape of leaves. Iwashimotsuke is vegetating in northern area than Kinki district, and Tosashimotsuke indigenously in Kochi prefecture. Thus at present Iwashimotsuke is named as Spiraea nipponica Maxim, Tosashimotsuke as S. nipponica Maxim var. tosaensis( Yatabe) Makino, and Kiishimotsuke as S. nipponica Maxim var. ogawae (Nakai) Yamanaka. In view of recent progress of molecular phylogenetics, the conventional method was employed to examine whether Kiishimotsuke is a species intimately related to the other two plants or not. The nucleotide sequences were determined for the regions of trn L – trn F of chloroplast DNA and ITS (Internal Transcribed Spacer) of 5S RNA coding region of nuclear DNA. The results showed that the differences in the sequences were less than 1% among the three, demonstrating their close evolutionary relationships. Thus it is reasonable to conclude that Kiishimotsuke is a variety or ecotype of S. nipponica based not only on morphological comparison but also on molecular phylogenetic analysis. Unexpectedly significant difference in the sequence of the ITS for Iwashimotsuke( Spiraea nipponica Maxim) was found between the one published by Potter et al.( DQ897622) and ours( LC133174 and LC13375). The difference remains to be elucidated. Since Kiishimotsuke is endemic to serpentine soils in Wakayama, the interaction of the plant with serpentine soils was also studied. When these three plants were raised from seeds on non-serpentine cultivation soils, all of them grew normally and the differences in the shape of the leaves were retained, indicating the shapes of leaves were not affected by the kind of soil but determined inherently. Nikkel ion is known to be one of the most toxic metal ions in serpentine soils. When the effects of increasing concentrations of Ni 2 + on the sprouting of seeds were tested, Kiishimotsuke was most tolerant among others. In the aboveground parts of Kiishimotsuke plants grown on serpentine soils of Mt. Ryumon, accumulation of Ni 2 + was confirmed and its possible significance of the plant survival was discussed.
  • Search of the C4 photosynthesis establishment genes using the C3/C4 photosynthesis interconversion plant Eleocharis vivipara, HARADA Daijiro, SAKAMOTO Tomoaki, KURATA Tetsuya, YAMATO Katsuyuki, IZUI Katsura, AKITA Motomu, BSJ-Review, BSJ-Review, 7A, 35 - 41, 2016 , Refereed
  • Effect of Light Condition on Growth and Alkaloid Production in Japanese Plum-yew, TAKAYAMA SHINSAKU, UEMATSU TOSHIAKI, AKITA MOTOMU, 植物環境工学, 植物環境工学, 26(1), 25-29 (J-STAGE), 2014 , Refereed
  • In Vitro Cryopreservation of Moss Protonema (Racomitrium japonicum), AKITA MOTOMU, SEKI DAISUKE, MURASE HARUHIKO, 植物環境工学, 植物環境工学, 26(1), 30-34 (J-STAGE), 2014 , Refereed
  • Application of Gas Permeable Film for Liquid Static Culture of Moss (Physcomitrella patens), TANIGAKI YUSUKE, HAMANAKA MASATO, NISHINO YUSUKE, YAMATO YOSHIYUKI, AKITA MOTOMU, 植物環境工学, 植物環境工学, 26(3), 160-162 (J-STAGE), 2014 , Refereed
  • Application of gas permeable film for cultivation of aquatic plants, Daijiro Harada, Masato Hamanaka, Ryoichi Tsuga, Motomu Akita, 5th IFAC Conference on Bio-Robotics, 5th IFAC Conference on Bio-Robotics, 46(4), 240 - 243, Mar. 2013 , Refereed
    Summary:Completely closed bags made with a gas permeable film are available for culture of two aquatic plants, Eleocharis vivipara and Sphagnum nemoreum. E. vivipara, the C3-C4 photosynthesis interconvertible plant, was successfully grown in a sugar free liquid medium and developed C3 type culms even under relatively high intensity of illumination. Growth of S. nemoreum was clearly stimulated in the bag using a sugar containing medium. The film system is promising for further study of responses to environmental factors such as intensity and wavelength of illumination.
  • Eleocharis viviparaからの効率的RNA抽出法の検討, HARADA DAIJIRO, IZUI KATSURA, AKITA MOTOMU, Mem Fac Biol Oriented Sci Technol Kinki Univ, Mem Fac Biol Oriented Sci Technol Kinki Univ, (30), 49 - 59, Sep. 2012 , Refereed
  • Plant Biotechnology for Industrial Production, TAKAYAMA SHINSAKU, MERA NOBUAKI, AKITA MOTOMU, 植物環境工学, 植物環境工学, 24(4), 224-232 (J-STAGE), 2012 , Refereed
  • 植物工場生産の高効率化と高付加価値化のための生物学的およびシステム工学的アプローチ, AKITA MOTOMU, SUZUKI TAKAHIRO, Mem Fac Biol Oriented Sci Technol Kinki Univ, Mem Fac Biol Oriented Sci Technol Kinki Univ, 26(26), 23 - 37, Sep. 2010 , Refereed
  • Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes, Li-mei Chen, Hiroya Yurimoto, Kun-zhi Li, Izumi Orita, Motomu Akita, Nobuo Kato, Yasuyoshi Sakai, Katsura Izui, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(3), 627 - 635, Mar. 2010 , Refereed
    Summary:Formaldehyde (HCHO) is an air pollutant suspected of being carcinogenic and a cause of sick-house syndrome. Microorganisms called methylotrophs, which can utilize reduced C-1 compounds such as methane and methanol, fix and assimilate HCHO, whereas most plants are unable to assimilate HCHO directly. We found that a bacterial formaldehyde-fixing pathway (ribulose monophosphate pathway) can be integrated as a bypass to the Calvin-Benson cycle in transgenic Arabidopsis thaliana and tobacco by genetic engineering. These plants showed enhanced tolerance to HCHO and enhanced capacity to eliminate gaseous HCHO by fixing it as a sugar phosphate. Our results provide a novel strategy for phytoremediation of HCHO pollution, and also represent the first step toward the production of plants that can assimilate natural gas-derived C-1 compounds.
  • スキムミルクなどを助剤とする植物体からの酵素タンパク質の抽出法の開発―Eleocharis viviparaのPEPの抽出を例として―, NAGAMATSU HIROAKI, SAKAGAMI AYA, YAMAZAKI YASUHIRO, YOSHIMURA KAZUE, SEKII AI, AKITA MOTOMU, IZUI KATSURA, Mem Fac Biol Oriented Sci Technol Kinki Univ, Mem Fac Biol Oriented Sci Technol Kinki Univ, (25), 7 - 16, Mar. 2010 , Refereed
  • Quickly-released peroxidase of moss in defense against fungal invaders, Mikko T. Lehtonen, Motomu Akita, Nisse Kalkkinen, Elina Ahola-Iivarinen, Gunilla Ronnholm, Panu Somervuo, Mattias Thelander, Jari P. T. Valkonen, NEW PHYTOLOGIST, NEW PHYTOLOGIST, 183(2), 432 - 443, 2009 , Refereed
    Summary:P>Mosses (Bryophyta) are nonvascular plants that constitute a large part of the photosynthesizing biomass and carbon storage on Earth. Little is known about how this important portion of flora maintains its health status. This study assessed whether the moss, Physcomitrella patens, responds to treatment with chitosan, a fungal cell wall-derived compound inducing defense against fungal pathogens in vascular plants. Application of chitosan to liquid culture of P. patens caused a rapid increase in peroxidase activity in the medium. For identification of the peroxidase(s), matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF)/MS, other methods and the whole-genome sequence of P. patens were utilized. Peroxidase gene knock-out mutants were made and inoculated with fungi. The peroxidase activity resulted from a single secreted class III peroxidase (Prx34) which belonged to a P. patens specific phylogenetic cluster in analysis of the 45 putative class III peroxidases of P. patens and those of Arabidopsis and rice. Saprophytic and pathogenic fungi isolated from another moss killed the Prx34 knockout mutants but did not damage wild-type P. patens. The data point out the first specific host factor that is pivotal for pathogen defense in a nonvascular plant. Furthermore, results provide conclusive evidence that class III peroxidases in plants are needed in defense against hostile invasion by fungi. New Phytologist (2009) 183:432-443doi: 10.1111/j.1469-8137.2009.02864.x.
  • カイコ細胞質多角体病ウイルス由来ポリヘドリン遺伝子のタバコBY‐2細胞における発現, AKITA MOTOMU, YAMAZAKI YASUHIRO, SAITO NAOYA, KURODA TOSHIAKI, Mem Sch Biol Orient Sci Technol Kinki Univ, Mem Sch Biol Orient Sci Technol Kinki Univ, 18(18), 9 - 14, Sep. 2006 , Refereed
    Summary:Polyhedrin of Bombyx mori cytoplasmic polyhedrosis virus is a protein that crystallizes and forms polyhedral particles in insect cells infected by the virus. We introduced the polyhedrin gene to Nicotiana tabacum BY-2 cells with the GUS-fused VP3 gene, encoding the viral protein that is encapsulated in the polyhedron, by an Agrobacterium mediated transformation. The expression vector was constructed to regulate these two genes under CaMV35S promoters. A NPTII expression cassette regulated by the nopaline synthase promoter was located on the same T-DNA region. Transformed BY-2 cells that proliferated well on solid medium were examined with a microscope but no polyhedra were distinguished in the cells even if the cells were GUS-stained. Since production of polyhedrin was confirmed by a western blotting analysis, it is necessary to investigate another suitable condition for crystallization of polyhedrin and encapsulation of VP3 into the polyhedra.
  • Mass propagation of cherry (Cerasus x yedoensis Matsum.) through shoot primordia, M. Akita, K. Negishi, A. Kitano, M. Iwasaki, R. Komae, Y. Ohta, T. Kuriu, T. Takii, PROCEEDINGS OF THE VTH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURE BREEDING, VOLS 1 AND 2, PROCEEDINGS OF THE VTH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURE BREEDING, VOLS 1 AND 2, 725(725), 579 - +, 2006 , Refereed
    Summary:A cherry (Cerasus x yedoensis Matsum.) was mass propagated using tissue culture techniques. Shoot primordia were efficiently induced on a solid MS medium containing 30 g L-1 sucrose, 0.5 mg L-1 BA, 0.1 mg L-1 IBA and 3 mg L-1 GA(3). The primordia were maintained and proliferated using the same medium or a liquid medium whose composition was the same with the solid medium except elimination of agar. The primordia were also successfully propagated in a bioreactor. Shoots were regenerated on a modified MS solid medium in which the concentration of major inorganic salts (KNO3, NH4NO3 and CaCl2) was reduced by half, and sucrose and hormone concentrations were the same as that of medium for the primordia. Root formation was clearly inhibited in this medium but it was stimulated by subculturing the plants on the medium without growth regulators. Plantlets were successfully acclimatized on 1:1 mixture of Kanuma-soil (a weathered pumice) and vermiculite. A culture scheme for efficient system for mass propagation of cherry is discussed.
  • 遺伝子組換え作物の安全性と倫理的考察, OTA YOSHIMOTO, AKITA MOTOMU, 近畿大学生物理工学部紀要, 近畿大学生物理工学部紀要, 15(15), 1 - 13, Mar. 2005 , Refereed
    Summary:Ancient Greek physician Hippocrates (460?-377? BC), the Father of Medicine, established an oath and asked the people who intend to be a physician to take the oath. In this Hippocratic oath starting as "I swear by Apollo Physician and Asclepius and Hygieia and Panaceia and all the gods and goddesses, making them my witnesses, that I will fulfil according to my ability and judgment this oath and this covenant : ", there is a sentence saying "...I will apply dietetic measures for the benefit of the sick according to my ability and judgment ; I will keep them from harm and injustice. ..." That is to say, life is respectable and the balance of benefit resulting from any actions to life must be over harm. The oath shows that, from those olden times, the importance of ethical consideration in relation to any actions of medicine affecting human life has been recognized by medical professionals. Today, scientists have made clear the subtle architecture of life in detail at the level of molecules, and have made it possible to manipulate genes of organism. They, as ancient Greek physician did, should consider seriously from ethical point of view about the balance between benefit and harm resulting from any actions applied to lives. This is particularly important for genetically modified (GM) crops, because crops are of fundamental importance for human life and their quality may influence directly human health. An ultimate benefit -good- resulting from GM crops is to ensure food for explosively increasing world population, and the confronting harm -bad- to this is uneasiness about safety as daily food and disturbance to natural environment. Even though scientists produced GM crops aiming at only the good, the final, decision to accept them will be made by the consumer. Thus, it is quite important to offer appropriate and understandable information about GM crop to the general public for making decision. In this respect, scientists who carry out the research at university have a responsibility to consider thoroughly on safety and ethical issue of GM crops and to educate students with appropriate manner. In this review, we discuss whether genetic modification of crops can be admitted ethically by trying to answer to public concern about the safety of transgenic crops as food and on environment.
  • Moss-Erwinia pathosystem reveals possible similarities in pathogenesis and pathogen defense in vascular and nonvascular plants, Robert A. Andersson, Motomu Akita, Minna Pirhonen, Elin Gammelgård, Jari P. T. Valkonen, Journal of General Plant Pathology, Journal of General Plant Pathology, 71(1), 23 - 28, Feb. 2005 , Refereed
    Summary:Vascular plants have various inducible resistance mechanisms as defense against pathogens. Mosses, small nonvascular plants (subkingdom Bryophyta), have been little studied in regard to their pathogens or modes of defense. Data here show that Erwinia carotovora, a bacterial plant pathogen that causes softrot in many dicotyledonous plants, can also cause soft rot symptoms in the moss Physcomitrella patens. Infection of moss by E. carotovora required pathogenicity factors similar to those required to infect vascular plants and, again as in vascular plants, salicylic acid (SA) induced moss to inhibit tissue maceration by Erwinia. These data reveal that SA-dependent defense pathways may have evolved before differentiation of vascular and nonvascular plants. © The Phytopathological Society of Japan and Springer-Verlag 2005.
  • Transformation of Leaf Lettuce (Lactuca sativa L.) by Infection of Seeds with Agrobacterium tumefaciens, AKITA MOTOMU, KAJIWARA HISASHI, OTA YOSHIMOTO, 植物工場学会誌, 植物工場学会誌, 16(4), 215 - 219, Dec. 2004 , Refereed
  • リーフレタス(Lactuca sativa L.)の形質転換系の確立, LUO S P, AKITA MOTOMU, OTA YOSHIMOTO, 近畿大学生物理工学部紀要, 近畿大学生物理工学部紀要, 12(12), 23 - 29, Sep. 2003 , Refereed
    Summary:An efficient method for transformation of leaf lettuce (Lactuca sativa L.) was developed. Leaf primodia-like tissue was established from the apical meristem under rotation culture using BA containing medium. Adventitious shoots with roots were efficiently induced from the tissue. When Agrobacterium tumefaciens harboring GUS gene was infected to the primodia-like tissue, shoots were generated on a selection medium and they clearly showed GUS activity.
  • 植物による異種タンパク質生産, OTA YOSHIMOTO, AKITA MOTOMU, 近畿大学生物理工学部紀要, 近畿大学生物理工学部紀要, 11(11), 1 - 23, Dec. 2002
    Summary:The utilization of transgenic plants for production of recombinant proteins and peptides of pharmaceutical interest has gained increasing attention over past decades. Plant expression systems have many potential advantages for producing these proteins. First, production cost is low. A large amount of raw material containing the target proteins are produced on an agricultural scale. Only sun light, water and fertilizer are required. Second, purification is easy or not necessary. The proteins can be expressed and accumulated in a particular compartment such as seeds or tubers. Edible plants may be utilized, in some cases, to deliver the protein directly. Third, plants are convinced to be safe hosts. Plant do not serve as hosts for human pathogens, and plant viruses are not pathogenic to humans.In this review, we discuss recent development in "molecular farming" for the production of biopharmaceuticals, edible vaccines, antibodies, and autoantigens for treatment of autoimmune diseases in transgenic plants.
  • A novel gene family in moss (Physcomitrella patens) shows sequence homology and a phylogenetic relationship with the TIR-NBS class of plant disease resistance genes, M Akita, JPT Valkonen, JOURNAL OF MOLECULAR EVOLUTION, JOURNAL OF MOLECULAR EVOLUTION, 55(5), 595 - 605, Nov. 2002 , Refereed
    Summary:Plant disease resistance (R) genes encode proteins in which several motifs of the nucleotide-binding region (NBS) are highly conserved. Using degenerate primers designed according to the kinase 1 (P-loop) and hydrophobic (HD) motifs of the R gene NBS domains, homologous sequences were cloned from moss (Physcomitrella patens; phylum Bryophyta) representing an ancient nonvascular plant. A novel gene family (PpC) with at least eight homologous members was found. Expression of five members was detected. The level of expression was dependent on the developmental stage of moss, being higher in the gametophyte tissue than in the protonema tissue. The PpCs contained the conserved motifs characteristic of the NBS regions of R genes, and a kinase domain was found upstream from the NBS region. Phylogenetic analysis using the deduced NBS amino acid sequences of the PpCs and the plant genes available in databanks indicated that the PpCs show the closest relationship with the TIR-NBS class of R genes. No significant similarity to plant genes other than R genes was observed. These findings shed novel light on the evolutionary history of the R gene families, suggesting that the NBS region characteristic of the TIR-NBS class of R-like genes evolved prior to the evolutionary differentiation of vascular and nonvascular plants.
  • A Simple Bioreactor System for Production of Storage Organs of Chinese Yam (Dioscorea opposita Thumb, Motomu Akita, Yoshimoto Ohta, Plant Biotechnology, Plant Biotechnology, 19(5), 353 - 356, 2002 , Refereed
    Summary:Storage organ of Dioscorea opposita was efficiently generated by using a drum type bioreactor. This reactor was not equipped with a system for mechanical aeration and explants were immersed intermittently into the medium on rotation. When explants precultured on a rotary shaker were transferred to the bioreactor, approx. 230 pieces of storable organs that could be bulbils or microtubers were generated after 5 weeks. These organs sprouted within a week and eventually grew into normal plants. © 2002, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.
  • A culture method to stimulate growth of root of sweet potato (Ipomoea batatas Lam)., YAMADA SHIHO, AKITA MOTOMU, OTA YOSHIMOTO, 近畿大学生物理工学研究所紀要, 近畿大学生物理工学研究所紀要, (6), 25 - 28, Jun. 2001 , Refereed
    Summary:Growth of root of sweet potato (Ipomoea batatas Lam) was significantly stimulated using a Ebb and Flow type culture system.
  • Production of Thymol by Shoot Culture of Thymus vulgaris., AKITA MOTOMU, OTA YOSHIMOTO, TAKAI GOKI, 植物工場学会誌, 植物工場学会誌, 12(1), 18 - 22, Mar. 2000 , Refereed
  • Estimation of quality of in vitro propagated corms of yam(Colocasia esculenta)., AKITA MOTOMU, OTA YOSHIMOTO, 近畿大学生物理工学研究所紀要, 近畿大学生物理工学研究所紀要, (2), 33 - 37, Mar. 1999 , Refereed
    Summary:継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University培養由来のサトイモ貯蔵器官(球茎)の苗質を評価する目的で、培養直後の球茎の乾燥耐性および球茎中に含まれる貯蔵物質(デンプン、レクチン)の含量を測定した。培養由来の球茎は、実験室内において速やかに萎れた。貯蔵物質の含量は市販の球茎よりも著しく低かった。これらの結果から、培養由来の球茎は貯蔵器官としての発達が不十分と判断された。培養条件によって貯蔵物質の含量は影響され、その含量は順化率に関係する可能性が示唆された。 (英文) Quality of in vitro propagated corms of yam (Colocasia esculenta cv. Ishikawawase) as the seed plants was estimated. In vitro derived corms were easily wilted when they were kept under the room condition. The contents of two kinds of reserve substances, starch and lectin, were significantly less than those in the field grown corms. These results indicate that development as the storage organ is insufficient in in vitro propagation of corms. Composition of the medium influenced on the content of these reserve substances which may affect on the efficiency of acclimatization of the corms.
  • Efficient selection of cells with high taxol content from heterogeneous Taxus cell suspensions by magnetic or fluorescent antibodies, M Kawamura, T Shigeoka, M Tahara, M Takami, H Ohashi, M Akita, Y Kobayashi, T Sakamoto, SEIBUTSU-KOGAKU KAISHI-JOURNAL OF THE SOCIETY FOR FERMENTATION AND BIOENGINEERING, SEIBUTSU-KOGAKU KAISHI-JOURNAL OF THE SOCIETY FOR FERMENTATION AND BIOENGINEERING, 76(1), 3 - 7, 1998 , Refereed
    Summary:Small cell clusters with high taxol contents were selected in the following manner. Cell suspensions derived from Taxus brevifolia and Taxus cuspidata were reacted with primary polyclonal antibodies of taxol, and then labeled by magnetic or fluorescent (FITC) secondary antibodies. Cell clusters bound to the secondary antibodies were picked up manually by a small magnet or through a fluorescence microscope. It was possible to seperate cell clusters having taxol contents of more than 1000 mu g/g DW from cell suspensions which had an average taxol content of 5 mu g/g DW pr less.
  • THE TYPES OF BIOREACTORS USED FOR SHOOTS AND EMBRYOS, S TAKAYAMA, M AKITA, PLANT CELL TISSUE AND ORGAN CULTURE, PLANT CELL TISSUE AND ORGAN CULTURE, 39(2), 147 - 156, Nov. 1994 , Refereed
    Summary:Plant regenerated organs such as shoots, bulbs, microtubers, corms, embryos, etc. have been successfully proliferated in the bioreactor. The use of a bioreactor leads to the development of technology suitable for large scale plant propagation. The basic construction and characteristics of various types of bioreactor systems are reviewed in relation to shoot and embryo cultures. A pilot scale 500 liter bioreactor system was applied to the production of large scale Stevia rebaudiana shoots.
  • Mass Propagation of Multiple Shoots Using a Large Bioreactor., AKITA MOTOMU, SHIGEOKA TAKEO, KOIZUMI YOKO, KAWAMURA MICHIO, 植物工場学会誌, 植物工場学会誌, 6(2), 113 - 121, Jun. 1994 , Refereed
  • Entrapment of Shoot Primordium into a Reticulate Form Matrix and Its Application for Shoot Mass Propagation., AKITA MOTOMU, KOIZUMI YOKO, KAWAMURA MICHIO, 植物工場学会誌, 植物工場学会誌, 6(2), 122 - 127, Jun. 1994 , Refereed
  • INDUCTION AND DEVELOPMENT OF POTATO-TUBERS IN A JAR FERMENTER, M AKITA, S TAKAYAMA, PLANT CELL TISSUE AND ORGAN CULTURE, PLANT CELL TISSUE AND ORGAN CULTURE, 36(2), 177 - 182, Feb. 1994 , Refereed
    Summary:Potato (Solanum tuberosum L.) tubers were mass propagated in a jar fermentor using a two-step method consisting of a shoot multiplication step (phase 1) followed by a tuber induction and development step (phase 2). Tuberization was observed within 2 weeks of phase 2 and the number of tubers did not increase after this culture period. In contrast, total tuber weight continuously increased for at least 10 weeks. Although the number of tubers and the total tuber weight clearly decreased under the lower temperature (17-degrees-C), this weight decrease was partially prevented by changing the temperature from 17-degrees-C to 25-degrees-C after 2 weeks of phase 2. This result indicates that tuber development can be controlled independently of induction. Lower temperatures influenced the localization and size distribution of tubers in the jar fermentor.
  • STIMULATION OF POTATO (SOLANUM-TUBEROSUM L) TUBERIZATION BY SEMICONTINUOUS LIQUID-MEDIUM SURFACE LEVEL CONTROL, M AKITA, S TAKAYAMA, PLANT CELL REPORTS, PLANT CELL REPORTS, 13(3-4), 184 - 187, Jan. 1994 , Refereed
    Summary:A procedure for the mass propagation of potato (Solanum tuberosum L.) tubers using a laboratory scale jar fermentor is described. Tuberization was clearly suppressed when the shoots were completely submerged in the liquid medium. Tubers were mainly formed at the medium surface. When shoots were cultured by the semi-continuous medium surface level control method, in which the medium surface level was raised or lowered throughout the culture period, tubers were induced and developed in every area in the jar fermentor. Tubers propagated by this method contained about 18 % (w/w) dry matter, slightly less than in field grown tubers, but most of the tubers weighing more than 0.2 g(FW) sprouted under room condition, without any acclimatization, during 3 month after the culture.
  • MASS PROPAGATION OF SHOOTS OF STEVIA-REBAUDIANA USING A LARGE-SCALE BIOREACTOR, M AKITA, T SHIGEOKA, Y KOIZUMI, M KAWAMURA, PLANT CELL REPORTS, PLANT CELL REPORTS, 13(3-4), 180 - 183, Jan. 1994 , Refereed
    Summary:A procedure for the mass propagation of multiple shoots of Stevia rebaudiana is described. Isolated shoot primordia were used as the inoculum to obtain clusters of shoot primordia. Such clusters were grown in a 500 liter bioreactor to obtain shoots. A total of 64.6 Kg of shoots were propagated from 460 g of the inoculated shoot primordia. These shoots were easily acclimatized in soil.
  • Resting period and the field performance of potato tubers propagated in a jar fermenter, AKITA Motomu, TAKAYAMA Shinsaku, 10, 255 - 259, Dec. 1993 , Refereed
  • Effects of medium surface level control on the mass propagation of potato tubers using a jar fermentor culture technnique, AKITA Motomu, TAKAYAMA Shinsaku, 10, 177 - 182, Dec. 1993 , Refereed
  • LARGE-SCALE PRODUCTION OF ANTHOCYANIN BY ARALIA-CORDATA CELL-SUSPENSION CULTURES, Y KOBAYASHI, M AKITA, K SAKAMOTO, HF LIU, T SHIGEOKA, T KOYANO, M KAWAMURA, T FURUYA, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40(2-3), 215 - 218, Nov. 1993 , Refereed
    Summary:The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradiation, was scaled up from flasks to a 10-1 glass jar fermenter, a 95-1 stainless steel jar fermenter, and finally a 500-1 pilot-scale jar fermenter. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0-17.2% (w/w) of the dried cells. In one of the operations of the 500-1 jar fermenter, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).
  • In situ measurement of the nitrification-denitrification activity in the rice rhizosphere., AKITA MOTOMU, YOSHIDA TOMIO, 日本土壌肥料学雑誌, 日本土壌肥料学雑誌, 58(4), 440 - 443, Aug. 1987 , Refereed
  • De novo short read assembly and functional annotation of eleocharis vivipara, a C3/C4 interconvertible sedge plant, Daijiro Harada, Katsuyuki T. Yamato, Katsura Izui, Motomu Akita, Environmental Control in Biology, Environmental Control in Biology, 56(2), 81 - 87, 2018 , Refereed
    Summary:Eleocharis vivipara is an amphibious sedge that displays C4 traits under terrestrial environments and C3 traits in submerged environments. This plant is thus potentially advantageous for screening genes indispensable to the development of C4 photosynthesis. In this study, we performed de novo transcriptome analysis of E. vivipara using its terrestrial- and submerged-type plants. By next-generation sequencing (NGS), approximately 90 and 89 million reads were yielded for the terrestrial and submerged types, respectively, and were assembled into 27,249 unigenes. Of these de novo consensus sequences, 94.5% showed similarities to database-registered sequences, and 69.4% were assigned with Gene Ontology terms. Our de novo assembled sequence data should provide a foundation for genetic analysis of the C4 photosynthetic system.
  • Physcomitrella patens Has Kinase-LRR R Gene Homologs and Interacting Proteins, Yusuke Tanigaki, Kenji Ito, Yoshiyuki Obuchi, Akiko Kosaka, Katsuyuki T. Yamato, Masahiro Okanami, Mikko T. Lehtonen, Jari P. T. Valkonen, Motomu Akita, PLOS ONE, PLOS ONE, 9(4), Apr. 2014 , Refereed
    Summary:Plant disease resistance gene (R gene)-like sequences were screened from the Physcomitrella patens genome. We found 603 kinase-like, 475 Nucleotide Binding Site (NBS)-like and 8594 Leucine Rich Repeat (LRR)-like sequences by homology searching using the respective domains of PpC24 (Accession No. BAD38895), which is a candidate kinase-NBS-LRR (kinase-NL) type R-like gene, as a reference. The positions of these domains in the genome were compared and 17 kinase-NLs were predicted. We also found four TIR-NBS-LRR (TIR-NL) sequences with homology to Arabidopsis TIR-NL (NM_001125847), but three out of the four TIR-NLs had tetratricopeptide repeats or a zinc finger domain in their predicted C-terminus. We also searched for kinase-LRR (KLR) type sequences by homology with rice OsXa21 and Arabidopsis thaliana FLS2. As a result, 16 KLRs with similarity to OsXa21 were found. In phylogenetic analysis of these 16 KLRs, PpKLR36, PpKLR39, PpKLR40, and PpKLR43 formed a cluster with OsXa21. These four PpKLRs had deduced transmembrane domain sequences and expression of all four was confirmed. We also found 14 homologs of rice OsXB3, which is known to interact with OsXa21 and is involved in signal transduction. Protein-protein interaction was observed between the four PpKLRs and at least two of the XB3 homologs in Y2H analysis.
  • Protein Secretome of Moss Plants (Physcomitrella patens) with Emphasis on Changes Induced by a Fungal Elicitor, Mikko T. Lehtonen, Yoshihiro Takikawa, Gunilla Ronnholm, Motomu Akita, Nisse Kalkkinen, Elina Ahola-Iivarinen, Panu Somervuo, Markku Varjosalo, Jari P. T. Valkonen, JOURNAL OF PROTEOME RESEARCH, JOURNAL OF PROTEOME RESEARCH, 13(2), 447 - 459, Feb. 2014 , Refereed
    Summary:Studies on extracellular proteins (ECPs) contribute to understanding of the multifunctional nature of apoplast. Unlike vascular plants (tracheophytes), little information about ECPs is available from nonvascular plants, such as mosses (bryophytes). In this study, moss plants (Physcomitrella patens) were grown in liquid culture and treated with chitosan, a water-soluble form of chitin that occurs in cell walls of fungi and insects and elicits pathogen defense in plants. ECPs released to the culture medium were compared between chitosan-treated and nontreated control cultures using quantitative mass spectrometry (Orbitrap) and 2-DELC-MS/MS. Over 400 secreted proteins were detected, of which 70% were homologous to ECPs reported in tracheophyte secretomes. Bioinformatics analyses using SignalP and SecretomeP predicted classical signal peptides for secretion (37%) or leaderless secretion (27%) for most ECPs of P. patens, but secretion of the remaining proteins (36%) could not be predicted using bioinformatics. Cultures treated with chitosan contained 72 proteins not found in untreated controls, whereas 27 proteins found in controls were not detected in chitosan-treated cultures. Pathogen defense-related proteins dominated in the secretome of P. patens, as reported in tracheophytes. These results advance knowledge on protein secretomes of plants by providing a comprehensive account of ECPs of a bryophyte.
  • Involvement of a Class III Peroxidase and the Mitochondrial Protein TSPO in Oxidative Burst Upon Treatment of Moss Plants with a Fungal Elicitor, Mikko T. Lehtonen, Motomu Akita, Wolfgang Frank, Ralf Reski, Jari P. T. Valkonen, MOLECULAR PLANT-MICROBE INTERACTIONS, MOLECULAR PLANT-MICROBE INTERACTIONS, 25(3), 363 - 371, Mar. 2012 , Refereed
    Summary:Production of apoplastic reactive oxygen species (ROS), or oxidative burst, is among the first responses of plants upon recognition of microorganisms. It requires peroxidase or NADPH oxidase (NOX) activity and factors maintaining cellular redox homeostasis. Here, PpTSPO1 involved in mitochondrial tetrapyrrole transport and abiotic (salt) stress tolerance was tested for its role in biotic stress in Physcomitrella patens, a nonvascular plant (moss). The fungal elicitor chitin caused an immediate oxidative burst in wild-type P. patens but not in the previously described delta Prx34 mutants lacking the chitin-responsive secreted class III peroxidase (Prx34). Oxidative burst in P. patens was associated with induction of the oxidative stress-related genes AOX, LOX7, and NOX, and also PpTSPO1. The available delta PpTSPO1 knockout mutants overexpressed AOX and LOX7 constitutively, produced 2.6-fold more ROS than wild-type P. patens, and exhibited increased sensitivity to a fungal necrotrophic pathogen and a saprophyte. These results indicate that Prx34, which is pivotal for antifungal resistance, catalyzes ROS production in P. patens, while PpTSPO1 controls redox homeostasis. The capacity of TSPO to bind harmful free heme and porphyrins and scavenge them through autophagy, as shown in Arabidopsis under abiotic stress, seems important to maintenance of the homeostasis required for efficient pathogen defense.
  • Practical Application of Methanol-Mediated Mutualistic Symbiosis between Methylobacterium Species and a Roof Greening Moss, Racomitrium, Akio Tani, Yuichiro Takai, Ikko Suzukawa, Motomu Akita, Haruhiko Murase, Kazuhide Kimbara, PLOS ONE, PLOS ONE, 7(3), e33800, Mar. 2012 , Refereed
    Summary:Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.
  • Fungi infecting cultivated moss can also cause diseases in crop plants, Mikko T. Lehtonen, Eeva M. Marttinen, Motomu Akita, Jari P. T. Valkonen, ANNALS OF APPLIED BIOLOGY, ANNALS OF APPLIED BIOLOGY, 160(3), 298 - 307, 2012 , Refereed
    Summary:Bryophytes (mosses) are non-vascular plants inhabited by a large number of fungal species, but whether mosses can act as reservoirs of fungal pathogens of crop plants has gained little attention. A few moss species including the Sunagoke moss (Racomitrium japonicum; family Grimmiaceae) are found to have modern economical applications in uses such as greening of urban environments. In a previous study, we identified fungi causing symptoms of varying severity in the commercially grown Sunagoke moss. The aim of this study was to test whether the same fungal isolates are pathogenic to vascular plants. An isolate of Fusarium avenaceum lethal to the Sunagoke moss caused root and crown rot in barley (Hordeum vulgare) and reduced germination of tomato (Solanum lycopersicum) and carrot (Daucus carota) grown in the infested soil. An isolate of Cladosporium oxysporum causing mild symptoms in moss reduced growth and caused reddening and premature death of carrot seedlings. On the other hand, isolates of Alternaria alternata and Fusarium oxysporum lethal to the Sunagoke moss caused no detectable symptoms in any tested vascular plant, suggesting specialisation of these isolates to moss. Chloroplast repositioning was observed in the neighbouring cells towards the initially infected cell following infection with F. avenaceum and A. alternata in Physcomitrella patens (family Funariaceae), a model moss used to study microscopic symptoms. Infection of P. patens with a non-virulent Apiospora montagnei isolate induced formation of papillae in the moss cells, indicating activation of host defence as described in vascular plants. Results suggest that mosses and vascular plants may be linked by a common microbial interface constituted by pathogenic fungi. The findings have epidemiological implications that have gained little previous attention.
  • Infection of the Sunagoke moss panels with fungal pathogens hampers sustainable greening in urban environments, Motomu Akita, Mikko T. Lehtonen, Hilkka Koponen, Eeva M. Marttinen, Jari P. T. Valkonen, SCIENCE OF THE TOTAL ENVIRONMENT, SCIENCE OF THE TOTAL ENVIRONMENT, 409(17), 3166 - 3173, Aug. 2011 , Refereed
    Summary:Drought and heat tolerance of the Sunagoke moss (Racomitrium japonicum) and the low thermal conductivity of the dry moss tissue offer novel greening and insulation possibilities of roofs and walls to mitigate the heat island phenomenon in urban environments. However, damage may appear in the moss panels under humid conditions in Japan. In this study we characterized fungi associated with the damaged areas of the Sunagoke moss panels. Fungi were identified by morphology and internal transcribed spacer (ITS) sequence analysis and tested for pathogenicity on R. japonicum (Grimmiaceae) and an unrelated moss species (Physcomitrella patens; Funariaceae) under controlled conditions. Alternaria alternata, Fusarium avenaceum and Fusarium oxysporum caused severe necrosis and death, whereas Cladosporium oxysporum and Epicoccum nigrum caused milder discoloration or chlorosis in both moss species. The fungi pathogenic on moss were closely related to fungal pathogens described from cultivated vascular plants. Ammonium increased severity of fungal diseases in moss. This study demonstrated that fungi can cause economically significant diseases in cultivated moss and hamper commercial use of the moss panels unless appropriate control methods are developed. Use of a single moss clone to cover large surfaces and the air pollutants such as ammonium may increase the risk for fungal disease problems. (C) 2011 Elsevier B.V. All rights reserved.
  • Culturable bacteria in hydroponic cultures of moss Racomitrium japonicum and their potential as biofertilizers for moss production, Akio Tani, Motomu Akita, Haruhiko Murase, Kazuhide Kimbara, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(1), 32 - 39, Jul. 2011 , Refereed
    Summary:The use of Racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. However, its utilization is hampered by slow growth rate. Here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. Most of the isolates belonged to Pseudomonas, Rhodococcus, and Duganella species. The isolates were biochemically characterized according to their type of interaction with plants, i.e., production of auxin, siderophores, or hydrogen cyanate, growth in the absence of an added nitrogen source, calcium phosphate solubilization, utilization of sugars, polymers, or aliphatic compounds, and antifungal activity. The isolates were applied to sterile protonemata and non-sterile adult gametophytes of R japonicum to evaluate their effect on plant growth. Furthermore, we isolated fungi that inhibited moss growth. Our results suggest that the microbial community structure in hydroponic cultures is important to stabilize moss production and the isolates that promote moss growth have potential to be utilized as biofertilizers for moss production. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
  • Practical aspects of bioreactor application in mass propagation of plants, S. Takayama, M. Akita, Liquid Culture Systems for in vitro Plant Propagation, Liquid Culture Systems for in vitro Plant Propagation, 61 - 78, 2005 , Refereed
    Summary:Bioreactors are an efficient tool for the production of plant propagules but, at present, their application is commercialized in only a few tissue culture companies. The present article reviews practical aspects of the use of bioreactors in the mass propagation of plants in relation to the responses of plant propagules in liquid medium, the characteristics of bioreactor culture techniques in plant propagation and discusses case studies of the use of bioreactors for several plant species including Fragaria ananassa, Lilium species, Hippeastrum hybridum, Gladiolus grandiflorus, Spathiphyllum, Colocasia esculenta and Solanum tuberosum. The establishment of plantlets from bioreactors and future prospects are also described.
  • The effect of Tiron, a water soluble radical scavenger, on growth, morphology and alkaloid content of adventitious roots in Atropa belladonna, M Akita, Y Ohta, PLANT CELL REPORTS, PLANT CELL REPORTS, 19(7), 705 - 709, Jun. 2000 , Refereed
    Summary:The effect of Tiron (disodium 1,2-dihydroxybenzene-3,5-disulfonate) on the growth, morphology and alkaloid content of adventitious roots in Atropa belladonna was investigated. High concentrations of Tiron had an inhibitory effect on growth of the root. The appearance of cultured roots was significantly changed from rough roots accompanied with callus-like tissue in control cultures to fine roots without callus formation. Alkaloid content was drastically increased by the addition of 1 mM Tiron to the medium. The influence of NAA, which has an inhibitory effect on alkaloid production, was partially restored by Tiron treatment, indicating that this radical scavenger may affect the production of alkaloids through modulation of the mode of action of auxin. Glutathione content of the root was not influenced by Tiron.
  • A simple method for mass propagation of potato (Solanum tuberosum L.) using a bioreactor without forced aeration, M Akita, Y Ohta, PLANT CELL REPORTS, PLANT CELL REPORTS, 18(3-4), 284 - 287, Dec. 1998 , Refereed
    Summary:We have developed a simple system for mass propagation of plant organs using a bioreactor without forced aeration. In this system, explants were cultured in bottles equipped with an air-permeable membrane on the cap and these bottles were slowly rotated on a bottle roller. Microtubers of potato were induced using a two-step culture method. In the first step, potato plantlets were cultured under static conditions. After shoot proliferation, the culture medium was replaced with a medium containing a higher concentration of sucrose and the bottles were rotated at 1 rpm. The number of tubers was clearly increased in this system compared to the culture without rotation. The results indicated that our system can be applied for mass propagation of potato tubers at low cost.
  • Newly developed apparatus for inoculating plant organs into large-scale fermentor, M Kawamura, T Shigeoka, M Akita, Y Kobayashi, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 82(6), 618 - 619, 1996 , Refereed
    Summary:We developed an apparatus for inoculation of a large number of plantlets or plant parts such as shoots or roots into a large-scale fermenter at one time. Ab opa belladonna adventitious roots (2.4 kg FW) were inoculated into a 500 l fermenter under sterile conditions, and 35 kg FW of the roots were obtained after 60-d cultivation. There were no differences in proliferation rate and scopolamine content between a 10 l fermenter culture used as control and the 500 l fermenter culture. The success of large-scale root culture was considered to be dependent on the use of the new inoculation apparatus.

Books etc

  • Practical aspects of bioreactor application in mass propagation (jointly worked), Liquid Culture Systems for in vitro Plant Propagation, Springer Verlag,   2005
  • Bioengineering aspects of bioreactor application in plant propagation (jointly worked), Plant Tissue Culture Engineering. (Focus on Biotechnology, Vol. 6), Springer Verlag,   2005
  • Bioreactor culture of plant organs, The Encyclopedia of Cell Technology,   2000

Conference Activities & Talks

  • Characterization of knockout mutant of KNLR1, a NBS-LRR gene of Physcomitrella patens, KURIMOTO Satoki, ISHIKURA Tomohiro, Mikko T. Lehtonen,Jari, P. T. Valkonen, AKITA Motomu, The 37th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2019 09 07
  • Production of Physcomitrella patens peroxidase(Prx34)by Pichia pastoris, TSURUKAWA Michiko, AKITA Motomu, NAKAYAMA Syohei, VALKONEN, P.T. Jari, The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2018 08 26
  • Study for establishment of transformation system of Eleocharis vivipara , a C3/C4 interconvertible sedge plant, FUKUNAGA Tatsuya, HARADA Daijiro, YAMAGUCHI Kazunari, AKITA Motomu, The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2018 08 26
  • Study for interaction between moss and moss associated bacteria, AKITA Motomu, The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2018 08 26
  • Pathogenicity of Sclerotium sp. to Physcomitrella patens, TANABE Minatsu, AKITA Motomu, The 36th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2018 08 26
  • Production of Physcomitrella patens peroxidase (Prx34) by Pichia pastoris, TSURUKAWA Michiko, AKITA Motomu, NAKAYAMA Syohei, VALKONEN Jari P.T, The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2017 08 29 , The Japanese Society of Plant Cell and Molecular Biology
  • Sclerotium delphinii is a harmful fungus to Racomitrium japonicum, TANABE Minatsu, MAKO Tamura, AKITA Motomu, The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2017 08 29 , The Japanese Society of Plant Cell and Molecular Biology
  • Physcomitrella patens activates defense responses against the pathogen Xanthomonas oryzae, SAKURAI Haruna, TAMURA Mako, AKITA Motomu, The 35th Annual Meeting of the Japanese Society of Plant Cell and Molecular Biology,   2017 08 29 , The Japanese Society of Plant Cell and Molecular Biology
  • Stimulation of bud induction of Racomitrium japonicum by CO2 enrichment and red light illumination, Motomu Akita, Miyuki Tachibana, Moss2016,   2016 09 02
  • Sclerotium as a harmful pathogen of sunagoke moss (Racomitrium japonicum) and its biological control using Bacillus, Mako Tamura, Minatsu Tanabe, Jari P. T. Valkonen, Motomu Akita, Moss2016,   2016 09 02
  • Improvement of water use efficiency (WUE) and drought tolerance in tobacco by genetic engineering: Further analyses by metabolomics profiling and δ13C measurement under stress, Takahide Nishimura, Hiroki Kitaoka, Yuko Takagi, Yasumune Nakayama, Yudai Denpo, Eiichiro Fukusaki, Ichiro Tayasu, Motomu Akita, Kathura Izui,   2015 03
  • C3/C4光合成相互転換植物Eleocharis viviparaを用いたC4光合成成立遺伝子の探索, HARADA DAIJIRO, SAKAMOTO TOMOAKI, KURATA TETSUYA, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物学会大会研究発表記録,   2014 09 01 , 招待有り
  • C4光合成のCO2代謝酵素及び輸送系の導入によるC3植物のC4化への試み―4種類の遺伝子を導入した組換え体トマトの作出―, NISHIMURA TAKAHIDE, HAYASHI KOKI, AKEDO ERISHU, TAKAGI YUKO, NAKAGAWA TSUYOSHI, TANIGUCHI MITSUTAKA, MATSUKURA CHIAKI, EZURA HIROSHI, TAYASU ICHIRO, AKITA MOTOMU, IZUI KATSURA, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2014 07 30
  • ‘C4化’タバコにおける水利用効率および乾燥ストレス耐性の向上:δ13Cの測定およびメタボローム解析とアミノ酸分析による性格付け, IZUI KATSURA, NISHIMURA TAKAHIDE, TAKAGI YUKO, AKEDO ERISHU, AKITA MOTOMU, ASHIDA HIROKI, YOKOTA AKIHO, TAYASU ICHIRO, NAKAYAMA YASUMUNE, DEMPO YUDAI, FUKUSAKI EIICHIRO, HIRANO HIROTO, MIWA TETSUYA, 日本植物生理学会年会要旨集,   2014 03 11
  • 次世代シーケンサーによるC3/C4光合成型Eleocharis viviparaのDe novoトランスクリプトーム解析, HARADA DAISHIRO, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2014 03 11
  • Findings of plant disease resistance gene (R-gene) homologues in moss (Physcomitrella patens), AKITA Motomu, MOLECULAR BIOLOGY AND BIOTIC INTERACTIONS OF MOSSES,   2013 10 31 , 招待有り
  • ヒヨス毛状根の生育とアルカロイドの代謝特性, TAKAYAMA SHINSAKU, MERA NOBUAKI, AKITA MOTOMU, 日本生物環境工学会大会講演要旨,   2013 09
  • イヌガヤの生育環境とアルカロイドの代謝特性, TAKAYAMA SHINSAKU, UEMATSU MASAAKI, KAWAMOTO HIROKI, FUJITA YU, AKITA MOTOMU, 日本生物環境工学会大会講演要旨,   2013 09
  • 酸素供給条件がヒヨス毛状根の培養特性に及ぼす影響, TAKAYAMA SHINSAKU, MERA NOBUAKI, AKITA MOTOMU, 日本生物環境工学会大会講演要旨,   2013 09
  • C3,C4光合成型相互転換植物Eleocharis viviparaの成長点におけるトランスクリプトーム解析, HARADA DAISHIRO, SAKAMOTO TOMOAKI, KURATA TETSUYA, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2013 09
  • Comparision of transcriptome between C3/C4 types of photosynthesis on Eleocharis vivipara by the next-generation sequencer, Daijiro Harada, Tomoaki Sakamoto, Tetsuya Kurata, Katsuyuki Yamato, Katsura Izui, Motomu Akita, The 16th International Congress on Photosynthesis,   2013 08
  • Physcomitrella patens has OsXa21 type membrane receptor homologs, Yusuke Tanigaki, Kenji Ito, Mikko T Lehtonen, Akiko Kosaka, Katsuyuki Yamato, Jari, P. T. Valkonen, Motomu Akita, The 16th Annual Moss International Conference,   2013 06
  • Characterization of the chitosan-responsive peroxidase Prx34 of Physcomitrella patens, Kenji Ito, Yusuke Tanigaki, Mikko T. Lehtonen, Jari P. T. Valkonen, Motomu Akita,   2013 06
  • Isolation of antagonistic bacterium against fungi from Sunagoke moss (Racomitrium japonicum), Yoshihiro Morita, Yusuke Tanigaki, Kenji Ito, Wataru Kobori, Akio Tani, Mikko T Lehtonen, Eeva Marttinen, Jari, P.T. Valkonen, Ikko Suzukawa, Haruhiko Murase, Motomu Akita, The 16th Annual Moss International Conference,   2013 06
  • Application of Gas Permeable Film for Cultivation of Aquatic Plant, Daijiro Harada, Masato, Hamanaka, Ryoichi Tsuga, Motomu Akita, 2013 IFAC Bio-Robotics Conference,   2013 03 27
  • 次世代シーケンサーによるC3/C4光合成型Eleocharis viviparaトランスクリプトームの比較, HARADA DAISHIRO, HIRATA ITSUKI, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2013 03 14
  • 光合成における13C/12Cの同位体分別の測定による“C4化”植物の性格づけ, IZUI KATSURA, AKEDO ERISHU, ASHIDA HIROKI, HASHIZUME KEISUKE, YOKOTA AKIHO, AKITA MOTOMU, TAYASU ICHIRO, 日本植物生理学会年会要旨集,   2013 03 14
  • C4ミニサイクルのC3植物(トマト)への導入による光合成能の増強を目指して, HAYASHI YOSHITAKA, AKEDO ERISHU, HASHIZUME KEISUKE, TAKAGI YUKO, NAKAGAWA TSUYOSHI, TANIGUCHI MITSUTAKA, MATSUKURA CHIAKI, EZURA HIROSHI, AKITA MOTOMU, IZUI KATSURA, 日本植物生理学会年会要旨集,   2013 03 14
  • Transcriptome analysis of C3-C4 photosynthesis interconversion plant Eleocharis vivipara using the next-generation sequencers, Daijiro Harada, Itsuki Hirata, Akane Sato, Katsuyuki Yamato, Katsura Izui, Motomu Akita, The 10th International Congress on Plant Molecular Biology,   2012 10
  • 和歌山県天然記念物キイシモツケの分子系統分類学的および生理形態的性格付け:イワシモツケおよびトサシモツケとの比較, AKEDO ERIKA, HIRATA TOMOKO, KAMII KAZUYUKI, TAKAGI YUKO, MIZUNO TAKAFUMI, KOBAYASHI MAKOTO, KOIKE TAKAYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本植物学会大会研究発表記録,   2012 09 14
  • ホスホエノールピルビン酸カルボキシキナーゼを高発現させたAtropa belladonnaの特性, MISAKI SHOGO, KAWAGUCHI YOSHIMI, TADA TOMOHIRO, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2012 08 03
  • C4ミニサイクルのC3植物(トマト)への導入による光合成能の増強を目指して, IZUI KATSURA, AKEDO ERISHU, TAKAGI YUKO, HASHIZUME KEISUKE, HAYASHI YOSHITAKA, NAGAMATSU HIROAKI, NAKAGAWA TSUYOSHI, TANIGUCHI MITSUTAKA, MATSUKURA CHIAKI, EZURA HIROSHI, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2012 08 03
  • Arabidopsis thaliana培養細胞によるC1資化植物作出モデル系の構築―ホルムアルデヒド固定のための遺伝子コンストラクト検討―, KUBO SHIGERU, AKEDO ERISHU, NAKAGAWA TSUYOSHI, ISHIHARA TAKESHI, KONISHI MINEKO, YANAGISAWA SHUICHI, KASAOKA MASAMI, MAKITA YUKO, TOYODA TETSURO, YURIMOTO HIROYA, SAKAI YASUYOSHI, AKITA MOTOMU, IZUI KATSURA, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2012 08 03
  • 次世代シーケンサーによるC3‐C4光合成相互転換植物Eleocharis viviparaのトランスクリプトーム解析, HARADA DAISHIRO, HIRATA ITSUKI, SATO AKANE, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2012 08 03
  • C3‐C4光合成相互転換植物Eleocharis viviparaのin vitroにおける光合成型の切換え, HARADA DAISHIRO, MIZOBATA NATSUKI, YOKOYAMA KANAKO, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2012 03 09
  • 葉緑体においてPEPカルボキシラーゼとPEPカルボキシキナーゼを過剰発現させることによるタバコ(C3植物)の高CO2条件下での水利用効率(WUE)の向上, IZUI KATSURA, ASHIDA HIROKI, HASHIZUME KEISUKE, ARIKAWA YOSHIHIRO, HAMAGUCHI YUKO, YOKOMASA TAKEYOSHI, YOKOTA AKIHO, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2012 03 09
  • キトサン処理時にヒメツリガネゴケ(Physcomitrella patens)で観察されるROS生成, ITO KENJI, TANIGAKI YUSUKE, LEHTONEN MIKKO, VALKONEN JARI, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2012 03 09
  • シロイヌナズナのホルムアルデヒドストレス応答と活性酸素種(ROS)の関与に関する解析, KUBO SHIGERU, SAKAKIBARA HITOSHI, KURUSU TAKAMITSU, KUCHITSU KAZUYUKI, YURIMOTO HIROYA, SAKAI YASUYOSHI, AKITA MOTOMU, YAMATO KATSUYUKI, IZUI KATSURA, 日本植物生理学会年会要旨集,   2012 03 09
  • C3‐C4光合成の相互転換植物Eleocharis viviparaの先端分裂組織からの培養系の誘導とin vitroにおけるC3型とC4型の切り換え, HARADA DAISHIRO, MIZOBATA NATSUKI, YOKOYAMA KANAKO, YOSHIMURA KAZUE, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • イネへのホルムアルデヒドの同化代謝系(リブロースモノリン酸経路)の導入, SUZUKI SHIORI, AKEDO ERISHU, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • ヒメツリガネゴケ(Physcomitrella patens)に見出された病害抵抗性遺伝子(R遺伝子)に関する研究, TANIGAKI YUSUKE, ITO KENJI, KOSAKA AKIKO, LEHTONEN MIKKO, THELANDER MATTIAS, YAMATO KATSUYUKI, AKITA MOTOMU, VALKONEN JARI P. T, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • ヒメツリガネゴケ(Physcomitrella patens)に見出された病害抵抗性遺伝子(R遺伝子)に関する研究, TANIGAKI YUSUKE, KOSAKA AKIKO, ITO KENJI, LEHTONEN MIKKO, THELANDER MATTIAS, KITSUKAWA MAI, AKITA MOTOMU, VALKONEN JARI, 日本植物生理学会年会要旨集,   2011 03 11
  • リブロースモノリン酸経路(RuMP)の2種類の酵素の融合体をコードする合成遺伝子を導入したイネの作成と解析, SUZUKI SHIORI, AKEDO ERISHU, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本農芸化学会大会講演要旨集,   2011 03 05
  • シロイヌナズナを用いた植物のホルムアルデヒド応答のトランスクリプトーム解析, KUBO SHIGERU, CHIN REIBAI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, KATO NOBUO, SAKAI YASUYOSHI, AKITA MOTOMU, IZUI KATSURA, 日本農芸化学会大会講演要旨集,   2011 03 05
  • スナゴケ(Racomitrium japonicum)から単離される微生物とそのスナゴケの生育に対する影響, AKITA MOTOMU, KOBORI KO, TANIGAKI YUSUKE, UDO HIROFUMI, MORITA YOSHIHIRO, TANI AKIO, SUZUKAWA KAZUYUKI, LEHTONEN MIKKO, VALKONEN JARI, MURASE HARUHIKO, 日本農芸化学会大会講演要旨集,   2011 03 05
  • C3/C4光合成の相互転換植物,カヤツリグサ科のEleocharis viviparaのcDNAライブラリーの作成とC4型PEPCのゲノムDNA塩基配列の決定, HARADA DAIJIRO, KONDO CHIE, YONEHARA RYO, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 生化学,   2011
  • イネとタバコへのホルムアルデヒド固定能の付与:C1微生物のリブロースモノリン酸経路(RuMP)の2つの酵素の葉緑体における高発現にむけて, AKEDO ERIKA, SUZUKI SHIORI, FUKUI TAKATO, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 生化学,   2011
  • Characterization of a plant disease resistance gene homolog (PpC24) of Physcomitrella patens, MOSS 2010, The 13th annual international conference,   2010 07 , MOSS 2010, The 13th annual international conference
  • Transcriptome Analysis of Formaldehyde Response in Wild-Type and Formaldehyde-Tolerant Transformant of Arabidopsis, International Conference on Arabidopsis Research 2010,   2010 06 , International Conference on Arabidopsis Research 2010
  • エゾスナゴケ(Racomitrium japonicum)無菌培養系の特性, KOBORI KO, SEKI DAISUKE, AKITA MOTOMU, MURASE HARUHIKO, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2009 07 29
  • スナゴケ(Racomitrium japonicum)に対する病原性を有するカビの単離, AKITA MOTOMU, KOBORI WATARU, MURASE HARUHIKO, LEHTONEN MIKKO, VALKONEN JARI, 日本農芸化学会大会講演要旨集,   2009 03 05
  • チオレドキシンを包埋した多角体を用いたタンパク質分離法の開発, TANIGAKI YUSUKE, MOTOHASHI KEN, HISABORI TOORU, IKEDA KEIKO, MORI HAJIME, IZUI KATSURA, AKITA MOTOMU, 生化学,   2008 12
  • 分子レベルから植物の応答を調べる(コケの病害応答を例にして), AKITA MOTOMU, LEHTONEN MIKKO, VALKONEN JARI P.T, 日本生物環境工学会大会講演要旨,   2008 09 07
  • Racomitrium canescens as a material for greening and physiological study, ○Motomu Akita, Haruhiko Murase, Akio Tani, Mikko Lehtonen, Jari PT Valkonen, MOSS 2008, The annual international symposium for moss experimental research 2008,   2008 08 18 , MOSS 2008, The annual international symposium for moss experimental research 2008
  • Physcomitrella responds to chitosan with release of a specific peroxidas, M.T. LEHTONEN, M. AKITA, N. KALKKINEN, E. AHOLA-IIVARINEN, G. RONNHOLM, P. SOMERVUO, M. THELANDER, J.P.T. VALKONEN, MOSS 2008, The annual international symposium for moss experimental research 2008,   2008 08 16
  • Physcomitrella responds to chitosan with release of a specific peroxidase, M.T. LEHTONEN, M. AKITA, N. KALKKINEN, E. AHOLA-IIVARINEN, G. RONNHOLM, M. THELANDER, J.P.T. VALKONEN, MOSS 2008, The annual international symposium for moss experimental research 2008,   2008 08 , MOSS 2008, The annual international symposium for moss experimental research 2008
  • スナゴケの生育を促進する微生物, TANI AKIO, AKITA MOTOMU, MURASE HARUHIKO, KANEHARA KAZUHIDE, 日本生物工学会大会講演要旨集,   2008 07 11
  • Molecular basis of microbial one-carbon metabolism. Plant-growth promotion by methylobacteria, Akio Tani, Motomu Akita, Kosuke Kawaguchi, Hiroya Yurimoto, Yasuyoshi Sakai, Haruhiko Murase, Kazuhide Kimbara, Gordon Conference,   2008 07 , Gordon Conference
  • C3,C4光合成転換植物(Eleocharis vivipara)のC4型ホスホエノールピルビン酸カルボキシラーゼ(PEPC)の抽出法の開発, SAKAGAMI AYA, YAMASAKI YASUCHIKA, YOSHIMURA KAZUE, AKITA MOTOMU, IZUI KATSURA, 日本植物生理学会年会要旨集,   2008 03 15
  • スナゴケのプロトプラスト培養法の開発, TAKAGI KAZUE, AKITA MOTOMU, MURASE OSAHIKO, 日本生物環境工学会大会講演要旨,   2007 06 25
  • 培養槽による胞子嚢からのシダの大量増殖, KATO KIYOTAKA, OKADA MIZUHO, AKITA MOTOMU, IZUI KATSURA, 日本生物環境工学会大会講演要旨,   2007 06 25
  • カイコ細胞質多角体病ウイルスの多角体を利用したタンパク質分離法の開発:チオレドキシンをベイトとして, KURODA TOSHIAKI, SAITO NAOYA, IKEDA KEIKO, MOTOHASHI TAKESHI, HISAHORI TOORU, IZUI KEI, MORI HAJIME, AKITA MOTOMU, 日本農芸化学会大会講演要旨集,   2006 03 05
  • ペチュニアの組織培養における通気と光照射条件が酸化ストレスとその防御におよぼす影響, TAKAYAMA SHINSAKU, IKEMOTO MARIKO, AKITA MOTOMU, OKI HISASHI, 農業環境工学関連学会合同大会講演要旨集(CD-ROM),   2006
  • ペチュニアの組織培養における通気と光照射条件が培養容器内のガス環境におよぼす影響, TAKAYAMA SHINSAKU, IKEMOTO MARIKO, TANI AKIRA, AKITA MOTOMU, OKI HISASHI, 農業環境工学関連学会合同大会講演要旨集(CD-ROM),   2006
  • ペチュニアの組織培養における通気と光照射条件が生育に及ぼす影響, TAKAYAMA SHINSAKU, IKEMOTO MARIKO, AKITA MOTOMU, OKI HISASHI, 農業環境工学関連学会合同大会講演要旨集(CD-ROM),   2006
  • レタスの種子感染による遺伝子組換え法, AKITA MOTOMU, 農業環境工学関連7学会合同大会講演要旨集,   2005 09 12
  • Usefulness of in vitro culture of moss,   2004 11 , 招待有り
  • ヒメツリガネゴケ(Physcomitrella patens)相同組換え系を利用した物質生産技術に関する研究, KOMAE RUMI, AKITA MOTOMU, OTA YOSHIMOTO, 日本農芸化学会大会講演要旨集,   2004 03 05
  • 植物のDNAやRNAを扱う意義と基本的方法, AKITA MOTOMU, 日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨,   2003 09 08
  • 各種前駆体投与がベラドンナ根のアルカロイド含量に及ぼす影響, WATANABE TAKASHI, AKITA MOTOMU, OTA YOSHIMOTO, 日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨,   2002 08 06
  • ヤマノイモの貯蔵組織の簡易大量培養法, AKITA MOTOMU, OTA YOSHIMOTO, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2002 07 29
  • ヒメツリガネゴケ(Physcomitrella patens)を利用した有用代謝物生産に関する研究 (第1報) 遺伝子組換え条件の検討, KOMAE RUMI, AKITA MOTOMU, OTA HARUCHIKA, VALKONEN J P T, 日本農芸化学会大会講演要旨集,   2002 03 05
  • カイコ細胞質多角体病ウイルスを利用した有用タンパク質生産に関する研究, HOSHINO MAKI, ICHIHOUSHI MOTOYA, AKITA MOTOMU, OTA HARUCHIKA, IKEDA KEIKO, MORI HAJIME, 日本農芸化学会大会講演要旨集,   2002 03 05
  • Development of culture techniques for mass propagation of corms of Colocasia esculenta, AKITA Motomu, OHTA Yoshimoto, Twelfth symposium of the International society for tropical root crops,   2000 09
  • Research on the production of taro (Colocasia antiquorum) seedling by tissue culture., AKITA MOTOMU, OTA YOSHIMOTO, 日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨,   1998 07
  • タイム(Thymus vulgaris)のシュート培養によるThymolの生産, AKITA MOTOMU, OTA YOSHIMOTO, TAKAI TAKASHIGE, 日本植物工場学会大会学術講演要旨集,   1998
  • Effect of reducing agents on alkaloid production in Atropa belladonna., AKITA MOTOMU, OGURA KAZUKI, NAGAO TOMOAKI, OTA YOSHIMOTO, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   1997 07
  • Development of a simple culture apparatus for mass culturing a plant storage tissue., AKITA MOTOMU, HATA MITSUNORI, KURIHARA MASAHIKO, OTA YOSHIMOTO, 日本農業気象学会全国大会日本生物環境調節学会大会合同大会講演要旨,   1997 06
  • Effect of tiron on growth and production of alkaloids in adventitious root culture of Atropa belladonna., AKITA MOTOMU, OTA YOSHIMOTO, 植物細胞分子生物シンポジウム講演要旨集,   1996 07
  • Mass culture of a plant organ using large-scale culture tank., AKITA MOTOMU, SHITA Rep,   1994 01 , 招待有り
  • 多孔質担体への植物組織の捕捉とその応用に関する研究, 秋田求, 小泉蓉子, 川村道生, 日本植物工場学会大会学術講演要旨集,   1993
  • Growth and alkaloid production of the entrapped roots of Atropa belladonna in a reticulated form matrix., 秋田求, 高山真策, 田中秀夫, 植物組織培養学会大会,シンポジウム講演要旨集,   1991 07
  • Mass propagation of potato tubers using tissue culture techniques., AKITA MOTOMU, TAKAYAMA SHINSAKU, 日本植物組織培養学会植物組織培養シンポジウム講演要旨集,   1988

Works

  • Joint Research on molecular biology of moss

Misc

  • Arabidopsis thaliana培養細胞によるC1資化植物作出モデル系の構築―ホルムアルデヒド固定のための遺伝子コンストラクト検討―, 久保森, 明渡絵里朱, 中川強, 石原健志, 小西美稲子, 柳澤修一, 笠岡昌美, 蒔田由布子, 豊田哲郎, 由里本博也, 阪井康能, 秋田求, 泉井桂, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 30th, 170,   2012 08 03 , http://jglobal.jst.go.jp/public/201302222465441701
  • シロイヌナズナのホルムアルデヒドストレス応答と活性酸素種(ROS)の関与に関する解析, 久保森, 榊原均, 来須孝光, 朽津和幸, 由理本博也, 阪井康能, 秋田求, 大和勝幸, 泉井桂, 日本植物生理学会年会要旨集, 53rd, 192,   2012 03 09 , http://jglobal.jst.go.jp/public/201202232539389233
  • イネへのホルムアルデヒドの同化代謝系(リブロースモノリン酸経路)の導入, 鈴木詩織, 明渡絵里朱, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 29th, 148,   2011 09 06 , http://jglobal.jst.go.jp/public/201102254893180968
  • シロイヌナズナを用いた植物のホルムアルデヒド応答のトランスクリプトーム解析, 久保森, 陳麗梅, 榊原均, 由里本博也, 加藤暢夫, 阪井康能, 秋田求, 泉井桂, 日本農芸化学会大会講演要旨集, 2011, 8,   2011 03 05 , http://jglobal.jst.go.jp/public/201102246431290633
  • リブロースモノリン酸経路(RuMP)の2種類の酵素の融合体をコードする合成遺伝子を導入したイネの作成と解析, 鈴木詩織, 明渡絵里朱, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 日本農芸化学会大会講演要旨集, 2011, 9,   2011 03 05 , http://jglobal.jst.go.jp/public/201102281041215630
  • イネとタバコへのホルムアルデヒド固定能の付与:C1微生物のリブロースモノリン酸経路(RuMP)の2つの酵素の葉緑体における高発現にむけて, 明渡絵里朱, 鈴木詩織, 福井崇人, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 生化学, ROMBUNNO.2P-0223,   2011 , http://jglobal.jst.go.jp/public/201202286720615620
  • Production of new-type genetic resources by gene manipulation and the technology development for its application. Search of the interaction between plant genetic resources and organisms of different species, and research on utilization of plant genetic re, OTA YOSHIMOTO, SOTOMURA BEN'ICHIRO, YUKINAGA HISAJIRO, WATANABE KAZUO, AKITA MOTOMU, HORIBATA AKIRA, 遺伝子操作による新型遺伝資源の生産とその応用に関する技術開発 平成8-12年度 近畿大学生物理工学研究所研究成果報告書, 389(1),389-689,   2001 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902171535044393
  • Plant Propagation through Jar Fermentor and Tank Culture Techniques., TAKAYAMA SHINSAKU, AKITA MOTOMU, 月刊組織培養, 20, 11, 413, 416,   1994 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902145116334739
  • Hormonal conditions in tissus culture of the potato., AKITA MOTOMU, TAKAYAMA SHINSAKU, ポテトサイエンス, 9, 3/4, 97, 98,   1989 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902014624306697
  • Mass propagation of cherry (Cerasus x yedoensis Matsum.) through shoot primordia, M. Akita, K. Negishi, A. Kitano, M. Iwasaki, R. Komae, Y. Ohta, T. Kuriu, T. Takii, PROCEEDINGS OF THE VTH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURE BREEDING, VOLS 1 AND 2, 40, 725, 579, +,   2006
    Summary:A cherry (Cerasus x yedoensis Matsum.) was mass propagated using tissue culture techniques. Shoot primordia were efficiently induced on a solid MS medium containing 30 g L-1 sucrose, 0.5 mg L-1 BA, 0.1 mg L-1 IBA and 3 mg L-1 GA(3). The primordia were maintained and proliferated using the same medium or a liquid medium whose composition was the same with the solid medium except elimination of agar. The primordia were also successfully propagated in a bioreactor. Shoots were regenerated on a modified MS solid medium in which the concentration of major inorganic salts (KNO3, NH4NO3 and CaCl2) was reduced by half, and sucrose and hormone concentrations were the same as that of medium for the primordia. Root formation was clearly inhibited in this medium but it was stimulated by subculturing the plants on the medium without growth regulators. Plantlets were successfully acclimatized on 1:1 mixture of Kanuma-soil (a weathered pumice) and vermiculite. A culture scheme for efficient system for mass propagation of cherry is discussed.
  • Moss-Erwinia pathosystem reveals possible similarities in pathogenesis and pathogen defense in vascular and nonvascular plants, Robert A. Andersson, Motomu Akita, Minna Pirhonen, Elin Gammelgård, Jari P. T. Valkonen, Journal of General Plant Pathology, 71, 1, 23, 28,   2005 02 , 10.1007/s10327-004-0154-3
    Summary:Vascular plants have various inducible resistance mechanisms as defense against pathogens. Mosses, small nonvascular plants (subkingdom Bryophyta), have been little studied in regard to their pathogens or modes of defense. Data here show that Erwinia carotovora, a bacterial plant pathogen that causes softrot in many dicotyledonous plants, can also cause soft rot symptoms in the moss Physcomitrella patens. Infection of moss by E. carotovora required pathogenicity factors similar to those required to infect vascular plants and, again as in vascular plants, salicylic acid (SA) induced moss to inhibit tissue maceration by Erwinia. These data reveal that SA-dependent defense pathways may have evolved before differentiation of vascular and nonvascular plants. © The Phytopathological Society of Japan and Springer-Verlag 2005.
  • Moss as a model system for studies on plant-pathogen interactions.(jointly worked), International Society for Molecular Plant-Microbe Interactions, 4, 244-247,   2004
  • Moss as a model system for studies on plant-pathogen interactions (jointly worked), Proceedings of the Ⅺ International Conference on Molecular Plant-microbe Interactions,   2003
  • A novel gene family in moss (Physcomitrella patens) shows sequence homology and a phylogenetic relationship with the TIR-NBS class of plant disease resistance genes, M Akita, JPT Valkonen, JOURNAL OF MOLECULAR EVOLUTION, 55, 5, 595, 605,   2002 11 , 10.1007/s002369-002-2355-8
    Summary:Plant disease resistance (R) genes encode proteins in which several motifs of the nucleotide-binding region (NBS) are highly conserved. Using degenerate primers designed according to the kinase 1 (P-loop) and hydrophobic (HD) motifs of the R gene NBS domains, homologous sequences were cloned from moss (Physcomitrella patens; phylum Bryophyta) representing an ancient nonvascular plant. A novel gene family (PpC) with at least eight homologous members was found. Expression of five members was detected. The level of expression was dependent on the developmental stage of moss, being higher in the gametophyte tissue than in the protonema tissue. The PpCs contained the conserved motifs characteristic of the NBS regions of R genes, and a kinase domain was found upstream from the NBS region. Phylogenetic analysis using the deduced NBS amino acid sequences of the PpCs and the plant genes available in databanks indicated that the PpCs show the closest relationship with the TIR-NBS class of R genes. No significant similarity to plant genes other than R genes was observed. These findings shed novel light on the evolutionary history of the R gene families, suggesting that the NBS region characteristic of the TIR-NBS class of R-like genes evolved prior to the evolutionary differentiation of vascular and nonvascular plants.
  • Development of culture techniques for mass propagation of Colocasia esculenta (jointly worked), Potential of root crops for food and industrial resources, 412-415,   2002
  • A Simple Bioreactor System for Production of Storage Organs of Chinese Yam (Dioscorea opposita thumb.)(jointly worked), Plant Biotechnology, 19(5), 353-356,   2002 , 10.5511/plantbiotechnology.19.353
  • A novel gene family in moss(Physcomitrella patens)shares high sequence homology with TIR-NBS class of plant disease resistance genes (jointly worked), Moss 2001 International Meeting on Moss Biology,   2001
  • Conditions for efficient protoplast release in moss(Physcomitrella patens)(jointly worked), Memoirs of the school of Biology-Oriented Science and Technology of Kinki University, 8, 49, 55,   2001
  • A simple method for mass propagation of potato (Solanum tuberosum L.) using a bioreactor without forced aeration, M Akita, Y Ohta, PLANT CELL REPORTS, 18, 3-4, 284, 287,   1998 12 , 10.1007/s002990050572
    Summary:We have developed a simple system for mass propagation of plant organs using a bioreactor without forced aeration. In this system, explants were cultured in bottles equipped with an air-permeable membrane on the cap and these bottles were slowly rotated on a bottle roller. Microtubers of potato were induced using a two-step culture method. In the first step, potato plantlets were cultured under static conditions. After shoot proliferation, the culture medium was replaced with a medium containing a higher concentration of sucrose and the bottles were rotated at 1 rpm. The number of tubers was clearly increased in this system compared to the culture without rotation. The results indicated that our system can be applied for mass propagation of potato tubers at low cost.
  • Generation of transgenic taro(Colocasia esculenta Schott) : I. Callus formation from etiolated-stem segments in taro cv. 'Ishikawawase. '(jointly worked), Breeding Science(suppl. ), 48, suppl 2, 274,   1998
  • Bioreactor techniques for large-scale culture of plant propagules (jointly worked), Advances in Horticultural science, 12, 2, 93, 100,   1998
  • Development of a bioreactor specialized for culture of differentiated tissue and organ of plants and its application for secondary metabolite production (jointly worked), Mem. School B. O. S. T. Kinki University, 1, 30, 39,   1997
  • Development of a system for mass propagation of Colocasia esculenta in large scale without forced aeration (jointly worked), Acta Horticulturae, 440, 554, 559,   1996
  • Bioreactor advances for the large-scale production of propagules.(jointly worked), COST 822,Workshop on somatic Embryogenesis,Artificial Seeds and Bioreactors., 2,   1996
  • Development of a system for mass propagation of Colocasia esculenta in large scale without forced aeration(jointly worked), Book of Abstracts International symposium on Plant Production in Closed Ecosystems -Automation,Celture,and Environment-, 125,   1996
  • THE TYPES OF BIOREACTORS USED FOR SHOOTS AND EMBRYOS, S TAKAYAMA, M AKITA, PLANT CELL TISSUE AND ORGAN CULTURE, 39, 2, 147, 156,   1994 11
    Summary:Plant regenerated organs such as shoots, bulbs, microtubers, corms, embryos, etc. have been successfully proliferated in the bioreactor. The use of a bioreactor leads to the development of technology suitable for large scale plant propagation. The basic construction and characteristics of various types of bioreactor systems are reviewed in relation to shoot and embryo cultures. A pilot scale 500 liter bioreactor system was applied to the production of large scale Stevia rebaudiana shoots.
  • Induction and development of potato tubers in a jar fermentor, Motomu Akita, Shinsaku Takayama, Plant Cell, Tissue and Organ Culture, 36, 2, 177, 182,   1994 02 , 10.1007/BF00037717
    Summary:Potato (Solanum tuberosum L.) tubers were mass propagated in a jar fermentor using a two-step method consisting of a shoot multiplication step (phase 1) followed by a tuber induction and development step (phase 2). Tuberization was observed within 2 weeks of phase 2 and the number of tubers did not increase after this culture period. In contrast, total tuber weight continuously increased for at least 10 weeks. Although the number of tubers and the total tuber weight clearly decreased under the lower temperature (17°C), this weight decrease was partially prevented by changing the temperature from 17°C to 25°C after 2 weeks of phase 2. This result indicates that tuber development can be controlled independently of induction. Lower temperatures influenced the localization and size distribution of tubers in the jar fermentor. © 1994 Kluwer Academic Publishers.
  • MASS PROPAGATION OF SHOOTS OF STEVIA-REBAUDIANA USING A LARGE-SCALE BIOREACTOR, M AKITA, T SHIGEOKA, Y KOIZUMI, M KAWAMURA, PLANT CELL REPORTS, 13, 3-4, 180, 183,   1994 01
    Summary:A procedure for the mass propagation of multiple shoots of Stevia rebaudiana is described. Isolated shoot primordia were used as the inoculum to obtain clusters of shoot primordia. Such clusters were grown in a 500 liter bioreactor to obtain shoots. A total of 64.6 Kg of shoots were propagated from 460 g of the inoculated shoot primordia. These shoots were easily acclimatized in soil.
  • Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinuous liquid medium surface level control, Motomu Akita, Shinsaku Takayama, Plant Cell Reports, 13, 3-4, 184, 187,   1994 01 , 10.1007/BF00239889
    Summary:A procedure for the mass propagation of potato (Solanum tuberosum L.) tubers using a laboratory scale jar fermentor is described. Tuberization was clearly suppressed when the shoots were completely submerged in the liquid medium. Tubers were mainly formed at the medium surface. When shoots were cultured by the semi-continuous medium surface level control method, in which the medium surface level was raised or lowered throughout the culture period, tubers were induced and developed in every area in the jar fermentor. Tubers propagated by this method contained about 18 % (w/w) dry matter, slightly less than in field grown tubers, but most of the tubers weighing more than 0.2 g(FW) sprouted under room condition, without any acclimatization, during 3 month after the culture. © 1994 Springer-Verlag.
  • LARGE-SCALE PRODUCTION OF ANTHOCYANIN BY ARALIA-CORDATA CELL-SUSPENSION CULTURES, Y KOBAYASHI, M AKITA, K SAKAMOTO, HF LIU, T SHIGEOKA, T KOYANO, M KAWAMURA, T FURUYA, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 40, 2-3, 215, 218,   1993 11
    Summary:The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradiation, was scaled up from flasks to a 10-1 glass jar fermenter, a 95-1 stainless steel jar fermenter, and finally a 500-1 pilot-scale jar fermenter. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0-17.2% (w/w) of the dried cells. In one of the operations of the 500-1 jar fermenter, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).
  • Resting period and the field performance of potato tubers propagated in a jar fermenter.(jointly worked), Motomu AKITA, Shinsaku TAKAYAMA, Plant Tissue Culture Letters., 10, 3, 255, 259,   1993 , 10.5511/plantbiotechnology1984.10.255
  • Effects of medium surface level control on the mass propagation of potato tubers using a jar fermentor culture technique.(jointly worked), Motomu AKITA, Shinsaku TAKAYAMA, Plant Tissue Culture Letters, 10, 3, 242, 248,   1993 , 10.5511/plantbiotechnology1984.10.242
  • Development of bioreactor specialized for differentiated plant tissue and organ cultures and its application for secondary metabolite production(jointly worked), International Symposium on Application of Biotechnological Methods and Recent Accomplishments of Economic Value in Asia,   1989
  • Mass propagation of potato tubers using jar fermentor techniques, (jointly worked), Acta Horticulturae, 230,55-61,   1988

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(B)), Production of plants for phytoremediation by metabolic engineering: Enhancement of an ability to remove environmental formaldehyde and methanol, Previously we succeeded in conferring an ability of absorbing and assimilating formaldehyde to plants by genetic engineering. In order to put this invention to practical use, (1) the improvements were made on the gene constructs to be expressed, (2) the molecular mechanism of toxicity of formaldehyde was partly elucidated, and (3) the applicability of this invention to a horticultural plant and a monocot plant, rice, was also demonstrated. As a step toward development of plants with an ability to assimilate methane, further introduction of the gene for methanol oxidase was also initiated.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(B)), Creation of plants that efficiently absorb and remove formaldehyde in air by genetic engineering, and its application to ornamental foliage plants, The two enzymes which are involved in the formaldehyde (HCHO) fixation in a methanol utilizing bacterium were expressed both in Arabidopsis (At) and tobacco. The obtained transformants showed an augmented capacity of absorbing HCHO and an enhanced tolerance to HCHO. Genome-wide analysis of the genes whose expression were affected in response to HCHO, gave clues to the mechanisms of toxicity of and tolerance to HCHO. The plasmids for the efficient expression of these enzyme genes in plants were constructed, and trials were made to obtain transgenic ornamental plants which will be useful for phytoremediation of HCHO-polluted indoor air.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(A)), Development of a simplified system for mass production of plant storage organs using tissue culture techniques., We attempted to develop a system for commercial production of storage organs of plants using a simplified bioreactor.Taro (Colocasia esculanta) and potato (Solanum tuberosum) were used as model plants in this study. Taro could be grown under a statical and completely submerged condition in the liquid medium. This indicates that taro can be cultured under the condition that the oxygen is supplied only by diffusion from the medium surface and forced aeration is not always necessary. Because forced aeration is thought to be one of the most complicated operations for large scale culture, elimination of the forced aeration is great advantage in simplifying the system. We designed a rotary drum type bioreactor in which the explants were cultured without forced aeration. Oxygen was supplied passively through a silicone spongy plug. Plants were entangled and fixed on a matrix inside of the bioreactor. By filling the bioreactor with a suitable volume of the liquid medium, plants were intermittently immersed into the medium by rotation. Corms of taro were successfully propagated in this system. Potato microtubers could also be mass propagated efficiently in this system.Cultured corms of taro were considered not to be mature because the content of storage protein and starch was significantly low. Taro corms could not be stored long term after finishing culture and their acclimatization just after the culture was easy, whereas root formation was not vigorous. The root formation was stimulated by culturing the corms in pure water or antioxidant solutions at the latest week of the culture and this increased the ratio of survived plants during cultivation was promoted.Our results indicate that the simplified culture system is applicable for mass propagation of corms of taro and microtubers of potato. Possible system for commercial production of the storage organs was discussed.
  • The Other Research Programs, Study on tissue culture techniques for production of plant secondary metabolites.
  • The Other Research Programs, study on control mechanism on plant secondary metabolism.
  • The Other Research Programs, Study on mass propagation methods by plant tissue culture techniques.
  • Research on techniques for production of plant metabolites
  • Research on bioreactor techniques for mass propagation of seedlings.