KINDAI UNIVERSITY


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MORIYAMA Ryutaro

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FacultyDepartment of Life Science
PositionLecturer
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/851-moriyama-ryutarou.html
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Last Updated :2020/06/01

Education and Career

Education

  •  - 2003 , Nagoya University
  •  - 1998 , Nagoya University
  •  - 1996 , Meiji University, School of Agriculture

Academic & Professional Experience

  •   2011 04 ,  - 現在, Faculty of Science and Engineering, Kindai University
  •   2011 09 ,  - 2012 09 , Visiting scientist, Department of Obstetrics & Gynecology, School of Medicine,University of Washington
  •   2007 04 ,  - 2011 03 , Faculty of Science and Engineering, Kindai University
  •   2005 04 ,  - 2007 03 , Faculty of Science and Engineering, Kindai University

Research Activities

Research Areas

  • Life sciences, Animal production science
  • Life sciences, Animals: biochemistry, physiology, behavioral science

Research Interests

  • Animal Physiology and Reproduction

Published Papers

  • Colocalization of GPR120 and anterior pituitary hormone-producing cells in female Japanese Black cattle., Nakamura S, Noda K, Miwa M, Minabe S, Hagiwara T, Hirasawa A, Matsuyama S, Moriyama R, The Journal of reproduction and development, The Journal of reproduction and development, Dec. 2019 , Refereed
  • Short-term but not long-term high-fat diet induces an increase in gene expression of gonadotropic hormones and GPR120 in the male mouse pituitary gland., Deura C, Moriyama R, The Journal of reproduction and development, The Journal of reproduction and development, Dec. 2019 , Refereed
  • AMP-activated protein kinase activation reduces the transcriptional activity of the murine luteinizing hormone β-subunit gene., Moriyama R, Iwamoto K, Hagiwara T, Yoshida S, Kato T, Kato Y, The Journal of reproduction and development, The Journal of reproduction and development, Dec. 2019 , Refereed
  • Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone beta-subunit gene, Ryutaro Moriyama, Tsubasa Yamazaki, Takako Kato, Yukio Kato, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 62(2), 195 - 199, Apr. 2016 , Refereed
    Summary:Here, we assessed the effects of long-chain fatty acids (LCFAs) and the LCFA receptor agonist GW9508 on the transcription of the gonadotropin subunit genes Cga, Lhb and Fshb because LCFA receptor GPR120 was observed in mouse gonadotropes in our recent study. A transcription assay using L beta T2 cells demonstrated that LCFAs, oleic acid, alpha-linolenic acid, docosahexaenoic acid and palmitate, repressed the expression of Cga, Lhb, and Fshb at concentrations between 50 and 100 mu M. On the other hand, treatment with 10 mu M unsaturated LCFAs, oleic acid, alpha-linolenic acid and docosahexaenoic acid, repressed only Fshb expression, while the same dose of a saturated LCFA, palmitate, had no effect on the expression of gonadotropin subunit genes. Furthermore, GW9508 did not affect promoter activity. Next, we examined deletion mutants of the upstream region of Fshb and found that the upstream regulatory region (-2824 to -2343 bp) of Fshb was responsible for the notable repression by 10 mu M unsaturated LCFAs. Our results suggest that the upstream region of Fshb is susceptible to unsaturated LCFAs. In addition, unsaturated LCFAs play a role in repressing Fshb expression through the distal -2824 to -2343 bp region, which might be independent of the LCFA receptor GPR120 pathway.
  • Expression of the long-chain fatty acid receptor GPR120 in the gonadotropes of the mouse anterior pituitary gland., Moriyama R, Deura C, Imoto S, Nose K, Fukushima N, Histochemistry and cell biology, Histochemistry and cell biology, 143(1), 21 - 27, Jan. 2015 , Refereed
  • A lysophosphatidic acid receptor lacking the PDZ-binding domain is constitutively active and stimulates cell proliferation., Shano S, Hatanaka K, Ninose S, Moriyama R, Tsujiuchi T, Fukushima N, Biochimica et biophysica acta, Biochimica et biophysica acta, 1783(5), 748 - 759, May 2008 , Refereed
  • Lysophosphatidic acid stimulates astrocyte proliferation through LPA1., Shano S, Moriyama R, Chun J, Fukushima N, Neurochemistry international, Neurochemistry international, 52(1-2), 216 - 220, Jan. 2008 , Refereed
  • Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids., Chikaya Deura, Yusuke Kimura, Takumi Nonoyama, Ryutaro Moriyama, The Journal of reproduction and development, The Journal of reproduction and development, Feb. 28 2020 , Refereed
    Summary:GPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. First, the effects of palmitate on GPR120, gonadotropic hormone b-subunits, and GnRH-receptor expression in gonadotropes were investigated in vitro. We observed palmitate-induced an increase in Gpr120 mRNA expression and a decrease in Fshb expression in LβT2 cells. Furthermore, palmitate exposure caused the phosphorylation of ERK1/2 in LβT2 cells, but no significant changes were observed in the expression levels of Lhb and Gnrh-r mRNA and number of GPR120 immunoreactive cells. Next, diurnal variation in Gpr120 mRNA expression in the male mouse pituitary gland was investigated using ad libitum and night-time restricted feeding (active phase from 1900 to 0700 h) treatments. In ad libitum feeding group mice, Gpr120 mRNA expression at 1700 h was transiently higher than that measured at other times, and the peak blood non-esterified fatty acid (NEFA) levels were observed from 1300 to 1500 h. These results were not observed in night-time-restricted feeding group mice. These results suggest that GPR120 is activated by LCFAs to regulate FSH synthesis in the mouse gonadotropes.
  • Effects of ovarian hormones on GPR120 mRNA expression in mouse pituitary gonadotrophs, Ryutaro Moriyama, Kaho Ueda, Chikaya Deura, ENDOCRINE JOURNAL, ENDOCRINE JOURNAL, 64(11), 1055 - 1061, 2017 , Refereed
    Summary:GPR120 is a G-protein-coupled receptor that is activated by long-chain fatty acids. In our previous study, GPR120 expression was detected in gonadotrophs of the mouse anterior pituitary gland. It is well known that the function of anterior pituitary cells is largely under the influence of circulating sex steroids. Thus, in the present study, we investigated the modulatory roles of the ovarian hormones, estrogen (E2) and progesterone (P), on the expression levels of GPR120 mRNA in mouse pituitary glands. GPR120 mRNA expression levels in the pituitary gland were increased after ovariectomy or P treatment, and were decreased after the administration of E2. Simultaneous injection of E2 and P interfered with the action of E2 on GPR120 mRNA expression. The GnRH antagonist, Cetrotide, did not inhibit the increase in GPR120 expression in ovariectomized (OVX) animals. In addition, immunohistochemistry revealed that more than 95.4% of GPR120 immunoreactive cells colocalized with the luteinizing hormone beta (LH beta) in the anterior pituitary gland of intact, ovariectomized (OVX), estradiol-primed OVX (OVX+E2), or progesterone-primed OVX (OVX+P) animals. Furthermore, GPR120 mRNA expression levels were not significantly different in the pituitary gland of females throughout the ovarian cycle. It is suggested that low levels of P may mask the inhibitory effect of estradiol on the synthesis of GPR120 in the estrous stage in intact animals. These results demonstrate that ovarian hormones may directly regulate GPR120 expression in the reproductive cycle at the pituitary level.
  • Redundancy in Kiss1 Expression Safeguards Reproduction in the Mouse, Simina M. Popa, Ryutaro M. Moriyama, Claudia S. Caligioni, Jasmine J. Yang, Caroline M. Cho, Tessa L. Concepcion, Amy E. Oakley, In Hae Lee, Elisenda Sanz, Paul S. Amieux, Alain Caraty, Richard D. Palmiter, Victor M. Navarro, Yee-Ming Chan, Stephanie B. Seminara, Donald K. Clifton, Robert A. Steiner, ENDOCRINOLOGY, ENDOCRINOLOGY, 154(8), 2784 - 2794, Aug. 2013 , Refereed
    Summary:Kisspeptin (Kiss1) signaling to GnRH neurons is widely acknowledged to be a prerequisite for puberty and reproduction. Animals lacking functional genes for either kisspeptin or its receptor exhibit low gonadotropin secretion and infertility. Paradoxically, a recent study reported that genetic ablation of nearly all Kiss1-expressing neurons (Kiss1 neurons) does not impair reproduction, arguing that neither Kiss1 neurons nor their products are essential for sexual maturation. We posited that only minute quantities of kisspeptin are sufficient to support reproduction. If this were the case, animals having dramatically reduced Kiss1 expression might retain fertility, testifying to the redundancy of Kiss1 neurons and their products. To test this hypothesis and to determine whether males and females differ in the required amount of kisspeptin needed for reproduction, we used a mouse (Kiss1-CreGFP) that has a severe reduction in Kiss1 expression. Mice that are heterozygous and homozygous for this allele (Kiss1(Cre/+) and Kiss1(Cre/Cre)) have similar to 50% and 95% reductions in Kiss1 transcript, respectively. We found that although male Kiss1(Cre/Cre) mice sire normal-sized litters, female Kiss1(Cre/Cre) mice exhibit significantly impaired fertility and ovulation. These observations suggest that males require only 5% of normal Kiss1 expression to be reproductively competent, whereas females require higher levels for reproductive success.
  • Lysophosphatidic acid induces neurite branch formation through LPA(3), Daisuke Furuta, Masayuki Yamane, Toshifumi Tsujiuchi, Ryutaro Moriyama, Nobuyuki Fukushima, MOLECULAR AND CELLULAR NEUROSCIENCE, MOLECULAR AND CELLULAR NEUROSCIENCE, 50(1), 21 - 34, May 2012 , Refereed
    Summary:Although neurite branching is crucial for neuronal network formation after birth, its underlying mechanisms remain unclear. Here, we demonstrate that lysophosphatidic acid (LPA) stimulates neurite branching through a novel signaling pathway. Treatment of neuronal cell lines with LPA resulted in neurite branch formation when LPA(3) receptor was introduced. The effects of LPA were blocked by inhibition of G(q) signaling. Furthermore, expression of inhibitory mutants of the small GTPase Rnd2/Rho7 or an Rnd2 effector rapostlin abolished LPA(3)-mediated neurite branching. The LPA(3) agonist 2(S)-OMPT or LPA also induced axonal branch formation in hippocampal neurons, which was blocked by G(q) and Rnd2 pathway inhibition or LPA(3) knockdown. These findings suggest that the novel signaling pathway involving LPA(3), G(q), and Rnd2 may play an important role in neuronal network formation. (c) 2012 Elsevier Inc. All rights reserved.
  • Post-translational modifications of tubulin in the nervous system, Nobuyuki Fukushima, Daisuke Furuta, Yuji Hidaka, Ryutaro Moriyama, Toshifumi Tsujiuchi, JOURNAL OF NEUROCHEMISTRY, JOURNAL OF NEUROCHEMISTRY, 109(3), 683 - 693, May 2009 , Refereed
    Summary:Many studies have shown that microtubules (MTs) interact with MT-associated proteins and motor proteins. These interactions are essential for the formation and maintenance of the polarized morphology of neurons and have been proposed to be regulated in part by highly diverse, unusual post-translational modifications (PTMs) of tubulin, including acetylation, tyrosination, detyrosination, Delta 2 modification, polyglutamylation, polyglycylation, palmitoylation, and phosphorylation. However, the precise mechanisms of PTM generation and the properties of modified MTs have been poorly understood until recently. Recent PTM research has uncovered the enzymes mediating tubulin PTMs and provided new insights into the regulation of MT-based functions. The identification of tubulin deacetylase and discovery of its specific inhibitors have paved the way to understand the roles of acetylated MTs in kinesin-mediated axonal transport and neurodegenerative diseases such as Huntington's disease. Studies with tubulin tyrosine ligase (TTL)-null mice have shown that tyrosinated MTs are essential in normal brain development. The discovery of TTL-like genes encoding polyglutamylase has led to the finding that polyglutamylated MTs which accumulate during brain development are involved in synapse vesicle transport or neurite outgrowth through interactions with motor proteins or MT-associated proteins, respectively. Here we review current exciting topics that are expected to advance MT research in the nervous system.
  • Lysophosphatidic acid stimulates neuronal differentiation of cortical neuroblasts through the LPA(1)-G(i/o) pathway, Nobuyuki Fukushima, Shinya Shano, Ryutaro Moriyama, Jerold Chun, NEUROCHEMISTRY INTERNATIONAL, NEUROCHEMISTRY INTERNATIONAL, 50(2), 302 - 307, Jan. 2007 , Refereed
    Summary:Lysophosphatidic acid (LPA) is an extracellular lipid mediator that regulates cortical development. Here we examined how LPA influences the cell fate of cortical neuroblasts using a neurosphere culture system. We generated neurospheres in the presence of basic fibroblast growth factor (bFGF). Treatment with LPA throughout the culture period significantly reduced the number of cells in the neurospheres. When dissociated single cells derived from neurospheres were induced to differentiate by adherence on coverslips, the proportion of MAP2-positive neurons was higher in LPA-treated neurospheres than in those treated with bFGF alone, and the proportion of myelin basic protein-positive oligodendrocytes was lower. Consistent with this finding, LPA raised the ratio of beta-tubulin type III-positive young neurons and reduced the ratio of CD140a-positive oligodendrocyte precursors in neurospheres. These effects of LPA were inhibited by pretreatment of neurospheres with pertussis toxin or an LPA(1)-preferring antagonist, Ki16425. Moreover, LPA-induced enhancement of neuronal differentiation was not observed in neurospheres derived from lpa(1)-null mice. These results suggest that LPA promotes the commitment of neuroblasts to the neural lineage through the LPA(1)-G(i/o) pathway. (c) 2006 Elsevier Ltd. All rights reserved.
  • In vitro increase in intracellular calcium concentrations induced by low or high extracellular glucose levels in ependymocytes and serotonergic neurons of the rat lower brainstem, R Moriyama, H Tsukamura, M Kinoshita, H Okazaki, Y Kato, KI Maeda, ENDOCRINOLOGY, ENDOCRINOLOGY, 145(5), 2507 - 2515, May 2004 , Refereed
    Summary:Pancreatic glucokinase (GK)-like immunoreactivities are located in ependymocytes and serotonergic neurons of the rat brain. The present study investigated in vitro changes in intracellular calcium concentrations ([Ca2+](i)) inresponse to low (2 mM) or high (20 mM) extracellular glucose concentrations in isolated cells from the wall of the central canal ( CC), raphe obscurus nucleus (ROb), ventromedial hypothalamus (VMH), and lateral hypothalamic area (LHA) in male rats. An increase in [Ca2+](i) was found in cells from the CC (21.1% or 9.8% of ependymocytes), ROb (10.9% or 14.5% of serotonergic neurons), VMH(7.8% and 25.2% of neurons), and LHA(20% or 15.7% of neurons), when extracellular glucose levels were changed from 10 to either 2 or 20 mM, respectively. Most of the ependymocytes and serotonergic neurons responding to the glucose changes were immunoreactive to the anti-GK in the CC (96.8% for low glucose and 100% for high glucose) and ROb (100% for low and high glucose). The [Ca2+](i) increase was blocked with calcium-free medium or L-type calcium channel blocker. Cells with an increase in [Ca2+](i) in response to low glucose did not respond to high glucose and vice versa. Inhibition of GK activity with acute alloxan treatment blocked low or high glucose-induced [Ca2+](i) increases in most GK-immunoreactive cells from the CC or ROb. The glucose- sensitive [Ca2+](i) increase in neurons of the VMH and LHA was also alloxan-sensitive, but no cells taken from the VMH and LHA were immunoreactive to the antibody used. The present study further indicates that ependymocytes of the CC and serotonergic neurons in the ROb are also sensitive to the changes in extracellular glucose in a GK-dependent manner, but that the subtype of GK in these cells could be different from that in the VMH and LHA.
  • A rat model for the energetic regulation of gonadotropin secretion: role of the glucose-sensing mechanism in the brain, M Kinoshita, R Moriyama, H Tsukamura, KI Maeda, DOMESTIC ANIMAL ENDOCRINOLOGY, DOMESTIC ANIMAL ENDOCRINOLOGY, 25(1), 109 - 120, Jul. 2003
    Summary:Energy availability has been considered to regulate gonadal activity by modulating the release of gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) at various reproductive phases, such as lactation and puberty in domestic as well as wild animals. Experimental models with rats and sheep have demonstrated that fasting or glucoprivation suppresses pulsatile LH release. From those experiments, the information on energy deficiency is considered to be detected by specific central sensors and conveyed to the hypothalamus to regulate LH release as well as food intake. Noradrenergic neurons, originating in the medulla oblongata and projecting to the hypothalamic paraventricular nucleus (PVN), is reported to be one of the pathways mediating the response of LH release to energy deficiency. The other component is considered to be an energy-sensing mechanism in the brain. Glucose or other oxidizable fuels may function as a metabolic signal to regulate LH release. Previous studies suggest the presence of a glucose-sensing mechanism in the rat hindbrain. From our previous results in the rat, the ependymocytes lining the wall of the cerebroventricle could possibly serve as a glucose sensor to regulate GnRH/LH release. Greater understanding of the nature of the energy-sensing mechanism in the brain will contribute to the nutritional manipulation of reproductive performance in domestic animals in various conditions. (C) 2003 Elsevier Inc. All rights reserved.
  • Glucoprivation-induced Fos expression in the hypothalamus and medulla oblongata in female rats, R Moriyama, BAS Reyes, H Tsukamura, K Maeda, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(2), 151 - 157, Apr. 2003 , Refereed
    Summary:Glucoprivation induced by 2-deoxy-D-glucose (2DG) suppresses pulsatile luteinizing hormone (LH) secretion in female rats. The suppression is enhanced in the presence of estrogen. In the present study, 2DG-induced Fos expression was examined in the solitary tract nucleus (NTS), hypothalamic paraventricular nucleus (PVN), raphe obscurus nucleus (ROb) and raphe pallidus nucleus (RPa), which have been previously suggested to be involved in glucoprivation-induced suppression of LH secretion in female rats. Ovariectomized (OVX) or estrogen-primed ovariectomized (OVX+E-2) rats were injected intravenously with 2DG (400 mg/kg BW). The brain was removed 1 h after the injection. The number of Fos-like-immunoreactive (Fos-li) cells in the PVN and NTS was significantly increased in OVX+E-2 rats compared with control groups, but did not show a significant increase in the OVX group. Few Fos-li cells were observed in the ROb and RPa in all groups. All of the Fos-li cells in the PVN and NTS were neurons because they had immunoreactivities to microtubule-associated protein 2. Some Fos-li cells (8.3%) had tyrosine hydroxylase-like immunoreactivities in the NTS in 2DG-treated OVX+E-2 rats. These results suggest that neurons in the PVN and NTS are involved in the estrogen-dependent neural cascade mediating glucoprivic suppression of LH secretion in female rats.
  • Intracerebroventricular administration of melanin-concentrating hormone suppresses pulsatile luteinizing hormone release in the female rat, H Tsukamura, RC Thompson, S Tsukahara, S Ohkura, F Maekawa, R Moriyama, Y Niwa, DL Foster, KI Maeda, JOURNAL OF NEUROENDOCRINOLOGY, JOURNAL OF NEUROENDOCRINOLOGY, 12(6), 529 - 534, Jun. 2000
    Summary:Melanin-concentrating hormone (MCH) has been reported to be involved in the regulation of feeding behaviour in rats and mice. Because many neuropeptides that influence ingestive behaviour also regulate reproductive function, the present study was designed to determine if central administration of MCH changes pulsatile secretion of luteinizing hormone (LH) in the rats. Wistar-Imamichi strain female rats were ovariectomized and implanted with oestradiol to produce a moderate inhibitory feedback effect on LH release. The effects of i.c.v. injections of MCH on LH release were examined in freely moving animals. Blood samples were collected every 6 min for 3 h through an indwelling cannula. After 1 h of sampling, MCH (0.1, 1 or 10 mu g/animal) or vehicle (saline) was injected into the third cerebroventricle. Because MCH is also reported to affect the hypothalamo-pituitary-adrenal (HPA) axis, which in turn, can influence reproductive function, plasma corticosterone concentrations were determined in the same animals at 30-min intervals during the first and last hours and every 12 min during the second hour of the 3-h sampling period. When expressed as per cent changes, mean plasma LH concentrations after MCH administration were significantly tower in the animals injected with all doses of the peptide compared with vehicle-treated animals; LH pulse frequency was significantly lowered by 1 mu g of MCH. Per cent changes in mean plasma corticosterone levels were not significantly affected by MCH administration. These results in oestradiol-treated ovariectomized rats indicate that central MCH is capable of inhibiting pulsatile LH secretion. We have previously shown that 48-h fasting suppresses pulsatile LH release in the presence of oestrogen, Take together, these results raise the possibility that MCH could play a role in mediating the suppression of LH secretion during periods of reduced nutrition.
  • Peripheral or central administration of motilin suppresses LH release in female rats: A novel role for motilin, H Tsukamura, S Tsukahara, F Maekawa, R Moriyama, BAS Reyes, T Sakai, Y Niwa, DL Foster, KI Maeda, JOURNAL OF NEUROENDOCRINOLOGY, JOURNAL OF NEUROENDOCRINOLOGY, 12(5), 403 - 408, May 2000
    Summary:Motilin is secreted in a clear episodic pattern during fasting or during the interdigestive phase, but feeding promptly stops this secretory pattern, and plasma concentrations of motilin decrease. We have previously determined that fasting markedly suppresses pulsatile luteinizing hormone (LH) secretion in female rats in the presence of oestrogen. In the present study, we wished to learn if motilin may mediate the fasting-induced suppression of LH secretion by determining the effects of motilin administration on LH release and on food intake. Intravenous (i.v.) injection of motilin (37 nmol/rat) suppressed LH release and significantly decreased mean LH concentrations both in ovariectomized (OVX) and oestradiol-implanted ovariectomized (OVX + E-2) rats. Food intake was significantly increased by i.v. motilin injection in OVX rats, but not in OVX + E-2 rats. It is likely that motilin inhibits LH release via inhibition of the gonadotrophin-releasing hormone (GnRH)-releasing mechanism at the hypothalamic level, because motilin (3.7 nmol/rat) also suppressed LH secretion when centrally administered, and because LH release in i.v. motilin-treated rats increased in response to exogenous GnRH. These results suggest that motilin may be a peripheral signal for the suppression of LH secretion through central sensors.

Conference Activities & Talks

  • Molecular mechanism of LPA3-mediated axonal branching formation in cultured hippocampal neurons,   2010 12
  • Lysophosphatidic acid receptor 3 activation induces axonal branch formation in cultured hippocampal neurons,   2010 09
  • Effect of fasting on GPR120 mRNA expression levels in the goldfish pituitary.,   2010 09
  • Long-chain fatty acids regulate GnRH receptor mRNA expression level in the gonadotrope of the mouse anterior pituitary gland., 43rd Annual Meeting of the Society for the Study of Reproduction,   2010 08 , 43rd Annual Meeting of the Society for the Study of Reproduction
  • Palmitate regulates GnRH receptor mRNA expression level in the gonadotrope of the mouse anterior pituitary gland., 14th International Congress of Endocrinology,   2010 03 , 14th International Congress of Endocrinology
  • 2-deoxy-D-glucose (2DG) induced glucoprivation suppressed gonadotropin-releasing hormone receptor (GnRH-R) and follicular stimulating hormone β subunit (FSHβ) mRNA expression levels in the LβT2 gonadotroph cells., 14th International Congress of Endocrinology,   2010 03 , 14th International Congress of Endocrinology
  • Lysophosphatidic acid receptor 3 is involved in neurite branching formation in hippocampal neurons,   2009 10
  • LPA induces neurite shape changes through LPA3, PLM2009,   2009 05 , PLM2009
  • Palmitate regulates GnRH receptor mRNA expression level in the gonadotrope of the mouse pituitary gland.,   2009 05
  • LPA1 mRNA expression level in mouse pituitary gonadotropes is enhanced by ovariectomy., 4th International Conference on Phospholipase A2 and Lipid Mediators,   2009 05 , 4th International Conference on Phospholipase A2 and Lipid Mediators
  • Palmitate regulates GnRH receptor mRNA expression in the gonadotrope of the mouse anterior pituitary,   2008 07
  • Fasting induced increase in long-chain fatty acid receptor GPR120 expression in the gonadotrope of the mouse anterior pituitary., 37th Annual Meeting of Society for Neuroscience,   2007 11 , 37th Annual Meeting of Society for Neuroscience
  • Palmitate-induced increase in long-chain fatty acid receptor GPR120 mRNA level in LβT2 pituitary gonadotrope cells.,   2007 09
  • Fasting induced Long-chain fatty acid receptor GPR120 expression increase in the anterior pituitary of mouse.,   2006 07

Misc

  • 不飽和脂肪酸によるマウス精子の運動活性化メカニズムについて, 森山 隆太郎, 佐藤 弘章, 山本 悠人, 楳田 千明, 山本 悠愛, 若狭 郁美, 萩原 央記, 和田 哲幸, The Journal of Reproduction and Development, 65, Suppl., j107, j107,   2019 09
  • 長鎖脂肪酸への曝露がウシおよびブタ精子の運動性に与える影響, 森山隆太郎, 平井直明, 野田航平, 山口直哉, 三輪雅史, 中村翔, 中村翔, 松山秀一, 松山秀一, Journal of Reproduction and Development, 64, Suppl Japanese Issue, j100, j100,   2018 09 05 , http://jglobal.jst.go.jp/public/201802246919139482
  • ウシ下垂体前葉におけるGPR120の局在, 中村翔, 中村翔, 野田航平, 野田航平, 三輪雅史, 美辺詩織, 松山秀一, 松山秀一, 森山隆太郎, 日本下垂体研究会学術集会プログラム・講演要旨集, 33rd, 48,   2018 , http://jglobal.jst.go.jp/public/201802215772779987
  • 妊娠成立によるマウス母体H‐P‐A軸のストレス応答性低下について, 森山隆太郎, 吉川万莉乃, 木村祐輔, 高野恭男, 向井久保崇裕, 松本彬伸, 日比野良祐, 日本下垂体研究会学術集会プログラム・講演要旨集, 32nd, 55,   2017 , http://jglobal.jst.go.jp/public/201702279152497228
  • マウス下垂体のゴナドトロフに局在する長鎖脂肪酸受容体GPR120発現におけるエストロジェンとプロジェステロンの役割, 上田 佳穂, 十河 由紀, 森山 隆太郎, The Journal of Reproduction and Development, 62, Suppl., j111, j111,   2016 09
  • マウス下垂体のゴナドトロフに局在する長鎖脂肪酸受容体GPR120発現におけるエストロジェンとプロジェステロンの役割, 上田佳穂, 十河由紀, 森山隆太郎, Journal of Reproduction and Development, 62, Suppl Japanese Issue, j111, j111,   2016 09 , http://jglobal.jst.go.jp/public/201702217065779799
  • ゴナドトロフにおけるリゾホスファチジン酸の役割検討, 森山隆太郎, 田近弘樹, J Reprod Dev, 61, Suppl Japanese Issue, J123, j123,   2015 09 05 , http://jglobal.jst.go.jp/public/201502213272557334
    Summary:【目的】リゾホスファチジン酸 (LPA)は,発生における細胞増殖促進や細胞形態変化などの生理活性を有するリゾリン脂質メディエーターである。LPA受容体はGタンパク質共役型で,6種類のサブタイプ (LPA1~LPA6)が知られている。また,成熟したほ乳類の血漿中にもLPAは存在するが,その生理的役割はあまり解明されていない。これまでの研究から,成熟マウス下垂体のα-subunit含有細胞に<i>Lpa1 </i>mRNAが発現していることを見出している。本研究では,性腺刺激ホルモン産生細胞 (ゴナドトロフ)におけるLPAの役割を検討する目的で実験を行った。 【方法】実験にはゴナドトロフ株化細胞LbT2を用いた。LPA刺激に細胞が反応するか検討するため,細胞内カルシウム濃度 ([Ca<sup>2+</sup>]i)を測定した。次に,ゴナドトロピン発現への関与を検討するため, LPA刺激によるα-subunitおよびゴナドトロピンLHβ, FSHβsubunit mRNA発現量を定量した。 【結果】細胞内カルシウム濃度を測定した結果, LPA 刺激によりLβT2の [Ca<sup>2+</sup>]iが上昇した。この上昇は細胞外カルシウム除去により阻害された。また, LPA刺激によりα-subunit mRNA発現量がコントロール群に比べて低下した。以上より, LPAは細胞外カルシウムを流入させる経路を介してゴナドトロフ株化細胞LβT2を活性化させることが明らかとなった。また, ゴナドトロピン発現に関与することが示唆された。
  • AMPK経路を介したLHβ鎖発現抑制, 森山隆太郎, 山崎翼, 中井愛, 加藤たか子, 加藤幸雄, 日本下垂体研究会学術集会プログラム・講演要旨集, 30th, 43,   2015 , http://jglobal.jst.go.jp/public/201502205386826634
  • グルコース利用性の低下が下垂体の性腺刺激ホルモン遺伝子の転写調節に与える影響, 森山隆太郎, 山崎翼, 中井愛, 加藤たか子, 加藤幸雄, J Reprod Dev, 60, Suppl Japanese Issue, J117, j117,   2014 08 10 , http://jglobal.jst.go.jp/public/201402237632885433
  • 性腺刺激ホルモン発現における長鎖脂肪酸の役割, 森山隆太郎, 山崎翼, 加藤たか子, 加藤幸雄, 日本下垂体研究会学術集会プログラム・講演要旨集, 29th, 41,   2014 , http://jglobal.jst.go.jp/public/201402293065321164
  • 長鎖脂肪酸への暴露がマウス精子の運動性に与える影響, 森山隆太郎, 若狭郁美, 中川雅子, J Reprod Dev, 59, Suppl Japanese Issue, J123, j123,   2013 08 20 , 10.14882/jrds.106.0.P-42.0, http://jglobal.jst.go.jp/public/201302267579915189
  • マウス下垂体のα‐subunit含有細胞に局在するリゾホスファチジン酸受容体1mRNAの発現量は去勢により増加する, 森山隆太郎, 松本絵美, 原玲奈, 宮里公子, 十河由紀, 福嶋伸之, 日本下垂体研究会学術集会プログラム・講演要旨集, 28th, 57,   2013 , http://jglobal.jst.go.jp/public/201302244884897670
  • 培養海馬神経細胞におけるLPA3を介する軸索分岐形成の分子メカニズム(Molecular mechanism of LPA3-Mediated axonal branching formation in cultured hippocampal neurons), 古田 大祐, 山根 昌之, 森山 隆太郎, 藤田 典久, 辻内 俊文, 福嶋 伸之, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 83回・33回, 2P, 0682,   2010 12
  • 絶食時のキンギョ脳下垂体におけるGPR120mRNA発現量について, 森山隆太郎, 宮前友紀子, 森井祐一郎, 加川尚, J Reprod Dev, 56, Suppl Japanese Issue, J110,   2010 08 20 , http://jglobal.jst.go.jp/public/201002286536595876
  • LPA3受容体活性化は海馬神経細胞の軸索分岐形成を引き起こす(Lysophosphatidic acid receptor 3 activation induces axonal branch formation in cultured hippocampal neurons), 福嶋 伸之, 古田 大祐, 山根 昌之, 辻内 俊文, 森山 隆太郎, 神経化学, 49, 2-3, 540, 540,   2010 08
  • クモ毒NPTX‐594のCa2+透過性グルタミン酸受容体に対する阻害効果について, 和田哲幸, 西丸貴弘, 和田祥子, 渡辺瑞貴, 宮本朋桂, 中尾真理子, 阪本大和, 船上仁範, 森山隆太郎, 若宮建昭, 西澤幹雄, 伊藤誠二, 吉田繁, 市田成志, 日本薬学会年会要旨集, 130th, 3, 146,   2010 03 05 , http://jglobal.jst.go.jp/public/201002256231291820
  • クモ毒NPTX-594のCa2+透過性グルタミン酸受容体に対する阻害効果について, 和田 哲幸, 西丸 貴弘, 和田 祥子, 渡辺 瑞貴, 宮本 朋佳, 中尾 真理子, 阪本 大和, 船上 仁範, 森山 隆太郎, 若宮 建昭, 西澤 幹雄, 伊藤 誠二, 吉田 繁, 市田 成志, 日本薬学会年会要旨集, 130年会, 3, 146, 146,   2010 03
  • Effect of fasting on GPR120 mRNA expression levels in the goldfish pituitary, MORIYAMA Ryutaro, MIYAMAE Yukiko, MORII Yuichiro, KAGAWA Nao, The Journal of Reproduction and Development Supplement, 103, 0, 104, 104,   2010 , http://ci.nii.ac.jp/naid/130007022391
    Summary:【目的】G-protein-coupled receptor 120 (GPR120) は血中遊離脂肪酸をリガンドとするGタンパク共役型受容体である。これまでに当研究室では、マウス下垂体の性腺刺激ホルモン産生細胞において、血中遊離脂肪酸濃度が上昇する絶食時にGPR120 mRNA発現量が増加することを明らかにしている。本研究では、マウスと同様に硬骨魚類であるキンギョ脳下垂体のGPR120も絶食時に発現量が増加すると仮説提唱し実験を行った。<BR>【方法】実験には1年齢の成熟和金を用いた。動物を正常給餌群と絶食群に分け、明暗と水温をコントロールした水槽で飼育した。10日間飼育した後、脳下垂体より抽出したRNAを用いてreal-time PCR法で<I>GPR120</I> mRNA発現量を定量した。また、絶食の効果を調べるために、飼育期間前後のキンギョの体重と血糖値・血中非エステル型脂肪酸 (NEFA) 濃度を測定した。<BR>【結果および考察】RT-PCR法およびサイクルシークエンス法によりキンギョ脳下垂体に<I>GPR120</I> mRNAの存在が明らかとなった。脳下垂体の<I>GPR120</I> mRNA発現量を定量した結果、絶食群の雄において正常給餌群に比べて統計的有意に<I>GPR120</I> mRNA発現量が増加した。一方、雌では統計的有意な増加は観察できなかった。また、血糖値と血中NEFA濃度を測定した結果、10日間の絶食による有意な濃度変化は観察できなかった。雄において<I>GPR120</I> mRNA発現量が統計的有意に増加したことから、マウスと同様に硬骨魚類であるキンギョにおいても脳下垂体のGPR120は、絶食時に何らかの生理的役割を持つと示唆された。ただし、絶食により血中NEFA濃度が増加しなかったことから、キンギョでは10日は絶食期間として不十分であり、そのため、雌で<I>GPR120</I> mRNA発現量の増加が観察できなかった可能性がある。現在、絶食期間を延ばした実験を行っている。
  • 培養海馬ニューロンにおいてリゾホスファチジン酸受容体3は神経突起分枝と関係がある(Lysophosphatidic acid receptor 3 is involved in neurite branching formation in cultured hippocampal neurons), 古田 大祐, 山根 昌之, 森山 隆太郎, 辻内 俊文, 福嶋 伸之, 日本生化学会大会プログラム・講演要旨集, 82回, 4P, 415,   2009 09
  • 高脂肪食給餌が性腺刺激ホルモン産生細胞の長鎖脂肪酸受容体Gpr120と性腺刺激ホルモンmRNA発現に与える影響, 出浦慎哉, 福嶋伸之, 森山隆太郎, J Reprod Dev, 55, Supplement, J130, j130,   2009 08 25 , 10.14882/jrds.102.0.1050.0, http://jglobal.jst.go.jp/public/200902234809881965
    Summary:【目的】マウス下垂体の性腺刺激ホルモン産生細胞(ゴナドトロフ)には長鎖脂肪酸受容体G protein-coupled receptor 120 (GPR120)が局在している。これまでに我々は、血中遊離脂肪酸が上昇する絶食時にマウス下垂体の<I>Gpr120</I> mRNA発現量が増加すること、パルミチン酸曝露によりゴナドトロフ株化細胞L&beta;T2で<I>Gpr120</I> mRNA発現量が増加し、<I>GnRH receptor</I> (<I>GnRH-R</I>) mRNA発現量が減少することを報告している(第101回 日本繁殖生物学会)。本研究では、高脂肪食給餌が下垂体の<I>Gpr120</I>、<I>GnRH-R</I>、<I>LH&beta;</I>、<I>FSH&beta;</I> mRNA発現量に与える影響を検討した。【方法】実験には12週齢のICR雄マウスを用いた。飼料に含まれる脂質:炭水化物:タンパク質のエネルギー比率は、10:70:20(コントロール食)と60:20: 20(高脂肪食)だった。1ヶ月間、自由摂食下で飼育した後、下垂体の<I>Gpr120</I>、<I>GnRH-R</I>、<I>LH&beta;</I>、<I>FSH&beta;</I> mRNA発現量をReal-time PCR法で測定した。【結果】1ヶ月間の給餌により高脂肪食群とコントロール群の体重はそれぞれ52.2 &plusmn; 3.4 gと42.2 &plusmn; 0.8 gになった。高脂肪食群ではコントロール群に比べて下垂体の<I>Gpr120</I>、<I>GnRH-R</I>、<I>FSH&beta;</I> mRNA発現量が統計的有意に増加した。また、<I>LH&beta;</I> mRNA発現量もコントロール群に比べて増加する傾向があった。以上の結果から、血中遊離脂肪酸が増加する生理的条件下では、ゴナドトロフの<I>Gpr120</I> mRNA発現量が増加すること、高脂肪食給餌が性腺刺激ホルモン、特にFSHの合成を促進することが示唆された。現在、高脂肪食給餌がマウスの血中LHとFSHのパルス状分泌に影響を与えるか検討している。
  • グルコースの利用阻害は下垂体の性腺刺激ホルモン産生細胞でGnRHレセプターおよびFSHβmRNA発現量を抑制する, 森山隆太郎, 中井愛, 福嶋伸之, J Reprod Dev, 55, Supplement, J129, j129,   2009 08 25 , 10.14882/jrds.102.0.1049.0, http://jglobal.jst.go.jp/public/200902253135496470
  • glucose availability suppresses GnRH receptor and FSHbeta mRNA expression in the gonadotrophs of the mouse pituitary gland, MORIYAMA Ryutaro, NAKAI Ai, FUKUSHIMA Nobuyuki, The Journal of Reproduction and Development Supplement, 102, 0, 1049, 1049,   2009 , http://ci.nii.ac.jp/naid/130007022586
    Summary:【目的】栄養は性腺機能を制御する因子の1つである。本研究ではマウス下垂体の性腺刺激ホルモン産生細胞 (ゴナドトロフ) が性腺機能を制御するエネルギーセンサーとして血糖値を感知すると仮説提唱して実験を行った。本研究の目的は、グルコースの拮抗利用阻害剤である2-deoxy-D-glucose (2DG) によりゴナドトロフの性腺刺激ホルモン放出ホルモン受容体 (GnRH-R) や性腺刺激ホルモン mRNA発現量が低下するか<I>in vitro</I>で検討することにある。【方法】 実験には8週齢のICR雄マウスとゴナドトロフ株化細胞L&beta;T2を用いた。下垂体とL&beta;T2でエネルギーセンシングに重要なAMP活性化プロテインキナーゼ (AMPK)、グルコキナーゼ (GK)、グルコース輸送担体2 (GLUT2)、GLUT4等のmRNAが発現しているかRT-PCR法で測定した。また、L&beta;T2を5mMの2DG に24時間暴露した後、GnRH-R、黄体形成ホルモン&beta;鎖 (LH&beta;)、卵胞刺激ホルモン&beta;鎖 (FSH&beta;) mRNA発現量の変化をreal-time PCR法で測定した。さらに、エネルギーセンサーとして知られるAMPKのリン酸化をwestern blot法で観察した。【結果】下垂体とL&beta;T2にはAMPK、GK、GLUT2、GLUT4等のmRNAが発現していた。また、2DG曝露によりL&beta;T2でAMPKのリン酸化が誘起され、GnRH-RとFSH&beta; mRNA発現量が低下した。しかし、AMPK活性剤であるAICARを投与した実験では、GnRH-RとFSH&beta; mRNA発現量の低下は観察できなかった。以上より、マウス下垂体にはグルコースを含めたエネルギー基質の感知メカニズムが存在すること、また、グルコース利用性の低下はゴナドトロフでGnRH-RとFSH&beta; mRNA発現量を低下させるが、そのメカニズムにAMPKのリン酸化は関与しないことが示唆された。
  • Palmitic acid regulates GnRH-receptor mRNA expression level in the mouse gonadotrope, Deura Chikaya, Hukusima Nobuyuki, Moriyama Ryutaro, The Journal of Reproduction and Development Supplement, 101, 0, 501, 501,   2008 , http://ci.nii.ac.jp/naid/130007022735
    Summary:【目的】長鎖脂肪酸はエネルギー基質であると同時に,シグナル分子としても機能する。これまでにマウス下垂体の性腺刺激ホルモン産生細胞 (ゴナドトロフ) に長鎖脂肪酸受容体G protein-coupled receptor 120 (GPR120) が発現することを明らかとしている。本研究の目的はゴナドトロフにおいて長鎖脂肪酸がGPR120を介して性腺機能を制御するか否か検討することにある。【方法】8週齢のICR雄マウス (点灯6時30分;消灯18時30分),マウス下垂体の初代培養細胞, ゴナドトロフ株化細胞L&beta;T2を用いた。明期にのみ餌を与える時間制限給餌を10日間行い,下垂体の<I>GPR120</I>および<I>GnRH-receptor</I> (<I>GnRH-R</I>) mRNA発現量の日内変動を測定した。ゴナドトロフに対する長鎖脂肪酸の作用を調べるため,下垂体の初代培養細胞とL&beta;T2細胞を用いて,パルミチン酸暴露による<I>GPR120</I>および<I>GnRH-R</I> mRNA発現量の変化を測定した。また,L&beta;T2細胞がパルミチン酸暴露によって受容体を介した細胞活性を起こすか,MAPキナーゼのリン酸化を指標として検討した。【結果】<I>GPR120</I>および<I>GnRH-R</I> mRNA発現量の日内変動を観察した結果, 正常給餌群では明期後半に<I>GPR120</I> mRNA 発現量の増加と,<I>GnRH-R</I> mRNA発現量の減少を観察した。一方,時間制限給餌群では正常給餌群で観察した<I>GPR120</I> mRNA 発現量の増加と<I>GnRH-R</I> mRNA発現量の減少は観察できなかった。パルミチン酸に暴露した下垂体の初代培養細胞とL&beta;T2細胞ではコントロール群に比べて<I>GPR120</I> mRNA 発現量が増加し,<I>GnRH-R</I> mRNA 発現量が減少した。さらに,L&beta;T2細胞においてパルミチン酸暴露後10分をピークとしたERKのリン酸化を検出したが,p38とJNKのリン酸化は検出できなかった。以上より,ゴナドトロフにおいて長鎖脂肪酸は何らかの受容体を介して<I>GnRH-R</I> mRNA発現量を制御することが示唆された。
  • グルコース利用阻害による視床下部室傍核と延髄でのFosタンパクの発現(Glucoprivation-Induced Fos Expression in the Hypothalamus and Medulla Oblongata in Female Rats), 森山 隆太郎, Reyes Beverly A.S, 束村 博子, 前多 敬一郎, The Journal of Reproduction and Development, 49, 2, 151, 157,   2003 04
  • 高グルコース及び低グルコース刺激に反応するラット延髄の膵臓型GK含有細胞, 森山 隆太郎, 束村 博子, 木下 美香, 吉田 恭子, 前多 敬一郎, The Journal of Reproduction and Development, 47, Suppl., a33, a33,   2001 12
  • Melanin-concentrating hormone(MCH)の脳室内投与はパルス状黄体形成ホルモン(LH)分泌を抑制する, 束村 博子, 塚原 伸治, 大蔵 聡, 前川 文彦, 森山 隆太郎, 前多 敬一郎, The Journal of Reproduction and Development, 45, Suppl., a36, a36,   1999 12
  • Expression of the glucose transporter in medulla oblongata area postrema related to the blood sugar percepting mechanism., 塚原伸治, THOMPSON R C, 束村博子, 森山隆太郎, FOSTER D L, 前多敬一郎, 日本畜産学会大会講演要旨, 94th, 107,   1998 03 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902138089433405
  • 血糖利用阻害に反応する延髄最後野及び孤束核の細胞, 森山 隆太郎, 日本畜産学会大会講演要旨集, 94回, 107, 107,   1998 03 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902122846176241
  • エストロジェンの摂食行動修飾作用のメカニズム, 束村博子, 前川文彦, 塚原伸治, 森山隆太郎, 前多敬一郎, 日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨, 23rd, 20,   1998 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902102293058112
  • Emission of LHRH from organotypic culture of hypothalamus tissue of rat newborn infant., 黒田朝生, 塚原伸治, 玉谷典華, 森山隆太郎, 束村博子, 前多敬一郎, 日本畜産学会大会講演要旨, 92nd, 133,   1997 03 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902120979336184
  • Hypoglycemia sensitive cell existing in brain., 森山隆太郎, 塚原伸治, 玉谷典華, 黒田朝生, 大宮恭子, 束村博子, 前多敬一郎, 日本畜産学会大会講演要旨, 92nd, 136,   1997 03 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902126563003249
  • LHRH secretion from the primary cell culture system of rat hypothalamic cells., 塚原伸治, 玉谷典華, 黒田朝生, 森山隆太郎, 束村博子, 前多敬一郎, 日本繁殖生物学会講演要旨, 89th, 104,   1996 10 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902193771277130

Research Grants & Projects

  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Effect of fatty acid quality on sperm motility
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), The regulatory mechanism of sperm motility using long-chain fatty acids as signal molecules, In the present study, activation of GPR120 induced increase in sperm motility via PI3K/Akt and p38 MAPK pathway. This evidence suggests that extracellular LCFAs act as signal molecles that regulate sperm motility through FFAR4.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), The mechanism for the hypoglycaemia induced suppression of gonadal function in the pituitary gland, In the present study, we observed the restriction of glucose availability induced the transcriptional suppression of luteinizing hormone beta subunit (LHbeta) and follicle-stimulating hormone beta subunit (FHSbeta) genes via AMP-activated protein kinase (AMPK) signaling pathway in the gonadotropic LbetaT2 cell line. The result suggests a possibility that gonadotrophs of the pituitary gland participate in nutritional disorders inducing reproductive dysfunction in many mammalian species from cattle to humans.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Molecular mechanisms for LPA_3-mediated neurite branch formation, Although neurite branching is crucial for neuronal network formation after birth, its underlying mechanisms remain unclear. Here, we demonstrate that lysophosphatidic acid(LPA) stimulates neurite branching through a novel signaling pathway. Treatment of neuronal cell lines with LPA resulted in neurite branch formation when LPA_3 receptor was introduced. The effects of LPA were blocked by inhibition of G_q signaling. Furthermore, expression of inhibitory mutants of the small GTPase Rnd2/Rho7 or an Rnd2 effector rapostlin abolished LPA_3-mediated neurite branching. The LPA_3 agonist 2(S)-OMPT or LPA also induced axonal branch formation in hippocampal neurons, which was blocked by G_q and Rnd2 pathway inhibition or LPA_3 knockdown. These findings suggest that the novel signaling pathway involving LPA_3, G_q, and Rnd2 may play an important role in neuronal network formation.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Gonadal function control mechanism in the pituitary gland through long-chain fatty acids as signal molecules., Present study showed that long-chain fatty acids such as palmitate may directly activate long-chain fatty acid receptor, GPR120, and down-regulate the synthesis of gonadotropin-releasing hormone receptors on gonadotrophs of the mouse pituitary gland as signal molecules. Moreover, it became clear that GPR120 expression levels were controlled by estrogen on the pituitary gland.