KINDAI UNIVERSITY


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YAMATO Katsuyuki

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FacultyDepartment of Biotechnological Science / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/499-yamato-katsuyuki.html
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Last Updated :2020/09/30

Education and Career

Education

  •   1984 04  - 1988 03 , Kyoto University, Faculty of Agriculture
  •   1988 04  - 1993 03 , Kyoto University

Academic & Professional Experience

  •   2016 04 ,  - 現在, professor, Faculty of Biology-Oriented Science and Technology, Kindai University
  •   2010 04 ,  - 2016 03 , associate professor, Faculty of Biology-Oriented Science and Technology, Kinki University
  •   1999 04 ,  - 2010 03 , assistant professor, Graduate School of Biostudies, Kyoto University
  •   1997 08 ,  - 1999 03 , assistant professor, Graduate School of Agriculture, Kyoto University
  •   1997 06 ,  - 1997 08 , postdoc, Division of Biological Sciences, University of Missouri, Columbia
  •   1995 06 ,  - 1997 05 , postdoc, Division of Biological Sciences, University of Missouri, Columbia
  •   1993 06 ,  - 1995 05 , postdoc, Division of Biological Sciences, University of Missouri, Columbia
  •   1993 04 ,  - 1993 05 , postdoc, Graduate School of Agriculture, Kyoto University

Research Activities

Research Areas

  • Life sciences, Applied molecular and cellular biology
  • Life sciences, Genomics
  • Life sciences, Systems genomics
  • Life sciences, Genomics
  • Life sciences, Applied biochemistry
  • Life sciences, Plants: molecular biology and physiology
  • Life sciences, Applied molecular and cellular biology

Research Interests

  • EST, RDA, Plant Molecular Biology

Published Papers

  • GEMMA CUP-ASSOCIATED MYB1, an Ortholog of Axillary Meristem Regulators, Is Essential in Vegetative Reproduction in Marchantia polymorpha., Yasui Y, Tsukamoto S, Sugaya T, Nishihama R, Wang Q, Kato H, Yamato KT, Fukaki H, Mimura T, Kubo H, Theres K, Kohchi T, Ishizaki K, Current Biology, Current Biology, 29(23), 3987 - 3995, Dec. 2019 , Refereed
  • A cis-acting bidirectional transcription switch controls sexual dimorphism in the liverwort., Hisanaga T, Okahashi K, Yamaoka S, Kajiwara T, Nishihama R, Shimamura M, Yamato KT, Bowman JL, Kohchi T, Nakajima K, The EMBO journal, The EMBO journal, 38(6), e100240, Jan. 2019 , Refereed
  • Cryopreservation of Marchantia polymorpha spermatozoa, Togawa T, Adachi T, Harada D, Mitani T, Tanaka D, Ishizaki K, Kohchi T, Yamato KT, Journal of Plant Research, Journal of Plant Research, 131(6), 1047 - 1054, Nov. 2018 , Refereed
    Summary:The liverwort <i>Marchantia polymorpha</i> has become one of the model organisms, since it has less genetic redundancy, sexual and asexual modes of reproduction and a range of genomic and molecular genetic resources. Cryopreservation of fertile spermatozoa eliminates time, space and labor for growing and maintaining male plants in reproductive phase, and also provides an optional way to backup lines. Here we report a protocol to cryopreserve spermatozoa of <i>M. polymorpha</i> in liquid nitrogen. A cryoprotective solution containing sucrose, glycerol and egg yolk and controlled cooling and warming processes led to successful recovery of motile <i>M. polymorpha</i> spermatozoa after the cryogenic process. The survival rate and average motility of spermatozoa after cryopreservation were maintained at 71 and 54% of those before cryopreservation, respectively. Cryopreserved spermatozoa were capable of fertilization to form normal spores. The technique presented here confers more versatility to experiments using <i>M. polymorpha</i> and could be applied to preservation of plant spermatozoa in general.
  • Loss of CG methylation in Marchantia polymorpha causes disorganization of cell division and reveals unique DNA methylation regulatory mechanisms of non-CG methylation., Ikeda Y, Nishihama R, Yamaoka S, Arteaga-Vazquez MA, Aguilar-Cruz A, Grimanelli D, Pogorelcnik R, Martienssen RA, Yamato KT, Kohchi T, Hirayama T, Mathieu O, Plant & Cell Physiology, Plant & Cell Physiology, 59(12), 2421 - 2431, Sep. 2018 , Refereed
  • Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha., Mano S, Nishihama R, Ishida S, Hikino K, Kondo M, Nishimura M, Yamato KT, Kohchi T, Nakagawa T, PLoS One, PLoS One, 13(10), e0204964, 2018 , Refereed
  • A Study on Kiishimotsuke, an Indigenous Plant to Wakayama Prefecture: Adaptation to Serpentine Soil and Molecular Phylogenetic Comparison with Iwashimotsuke and Tosashimotsuke, 明渡絵里朱, 平田智子, 上井和幸, 高木祐子, 水野隆文, 水野直治, 小林真, 小池孝良, 大和勝幸, 大和勝幸, 秋田求, 秋田求, 泉井桂, 泉井桂, 近畿大学先端技術総合研究所紀要, 近畿大学先端技術総合研究所紀要, (21), 33‐48, May 2016 , Refereed
  • Search of the C4 photosynthesis establishment genes using the C3/C4 photosynthesis interconversion plant Eleocharis vivipara, HARADA Daijiro, SAKAMOTO Tomoaki, KURATA Tetsuya, YAMATO Katsuyuki, IZUI Katsura, AKITA Motomu, BSJ-Review, BSJ-Review, 7A, 35 - 41, 2016 , Refereed
  • Cryopreservation of gemmae from the liverwort, Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato, Cryobiology, Cryobiology, 71(3), 560, 2015 , Refereed
  • The Liverwort Marchantia polymorpha L. as a novel model organism for reproduction research, Yamato Katsuyuki T, Journal of crop research, Journal of crop research, (59), 1 - 10, Jul. 2014
    Summary:The liverwort Marchantia polymorpha L. is one of extant species of the first land plants that appeared about 450 Myr ago. The genomes of plastid, mitochondria and Y chromosome in M. polymorpha were the first to be published among all plant species, making significant contributions to plant biology. M. polymorpha has now become a fascinating model organism for plant biology, because of its crucial position in the evolution of land plants and molecular genetic tools that have been recently developed. M. polymorpha is dioecious, and its complete haploid set of chromosomes (approximately 280 Mb) consists of eight autosomes and a single sex chromosome: an X chromosome for a female (n=8+X) and a Y chromosome for a male (n=8+Y). For sexual reproduction, male plants of M. polymorpha produce biflagellated spermatozoa, which swim in the water toward female plants to fertilize eggs. How spermatozoa detect, approach, and unite with the eggs at the molecular level is still a major issue in biology. A number of organisms, mostly animals and some algae, have been intensively studied to address these questions. Only a few of them, however, receive the advantage of molecular genetic approaches, such as genomic resources, genetics, transformation and gene targeting. M. polymorpha is one such organism with a variety of molecular genetic tools. The potential of M. polymorpha as a model organism for reproduction research will be discussed.
  • Plastid transformation of sporelings and suspension-cultured cells from the liverwort Marchantia polymorpha L., Chiyoda S, Yamato KT, Kohchi T, Methods in Molecular Biology, Methods in Molecular Biology, 1132, 439 - 447, 2014 , Refereed
  • Preview of the Marchantia genome, BSJ Review, BSJ Review, 3, 71 - 83, Jul. 2012
  • The basics of cultivating the liverwort Marchantia polymorpha L., YAMATO Katsuyuki, ISHIZAKI Kimitsune, KOHCHI Takayuki, Low Temperature Science, Low Temperature Science, 67, 23 - 29, Mar. 2009
  • MpFAE3, a beta-Ketoacyl-CoA synthase gene in the liverwort Marchantia polymorpha L., is preferentially involved in elongation of palmitic acid to stearic acid, M Kajikawa, KT Yamato, H Kanamaru, E Sakuradani, S Shimizu, H Fukuzawa, Y Sakai, K Ohyama, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67(8), 1667 - 1674, Aug. 2003 , Refereed
    Summary:Fatty acid chain elongation is a crucial step in the biosynthesis of long chain fatty acids. An essential reaction in the elongation process is condensation of malonyl-CoA with acyl-CoA, which is catalyzed by beta-ketoacyl-CoA synthase (KCS) in plants. We have isolated and characterized the MpFAE3 gene, one of the KCS gene family in the liverwort Marchantia polymorpha. Transgenic M. polymorpha plants overexpressing MpFAE3 accumulate fatty acids 18:0, 20:0, and 22:0. In these plants, the amount of 16:0 is reduced to 50% of wild type. In a heterologous assay, transgenic methylotrophic yeast expressing the MpFAE3 gene accumulates fatty acid 18:0 and generates several longer fatty acids which are not detectable in the control, accompanied by a decrease of 16:0. These observations indicate that the MpFAE3 protein is preferentially involved in the elongation of 16:0 to 18:0 and also in the subsequent steps of 18:0 to 20:0 and 20:0 to 22:0 in M. polymorpha.
  • Regulation of the Poly(A) Status of Mitochondrial mRNA by Poly(A)-Specific Ribonuclease Is Conserved among Land Plants., Mai Kanazawa, Yoko Ikeda, Ryuichi Nishihama, Shohei Yamaoka, Nam-Hee Lee, Katsuyuki T Yamato, Takayuki Kohchi, Takashi Hirayama, Plant & cell physiology, Plant & cell physiology, 61(3), 470 - 480, Mar. 01 2020 , Refereed
    Summary:Regulation of the stability and the quality of mitochondrial RNA is essential for the maintenance of mitochondrial and cellular functions in eukaryotes. We have previously reported that the eukaryotic poly(A)-specific ribonuclease (PARN) and the prokaryotic poly(A) polymerase encoded by AHG2 and AGS1, respectively, coordinately regulate the poly(A) status and the stability of mitochondrial mRNA in Arabidopsis. Mitochondrial function of PARN has not been reported in any other eukaryotes. To know how much this PARN-based mitochondrial mRNA regulation is conserved among plants, we studied the AHG2 and AGS1 counterparts of the liverwort, Marchantia polymorpha, a member of basal land plant lineage. We found that M. polymorpha has one ortholog each for AHG2 and AGS1, named MpAHG2 and MpAGS1, respectively. Their Citrine-fused proteins were detected in mitochondria of the liverwort. Molecular genetic analysis showed that MpAHG2 is essential and functionally interacts with MpAGS1 as observed in Arabidopsis. A recombinant MpAHG2 protein had a deadenylase activity in vitro. Overexpression of MpAGS1 and the reduced expression of MpAHG2 caused an accumulation of polyadenylated Mpcox1 mRNA. Furthermore, MpAHG2 suppressed Arabidopsis ahg2-1 mutant phenotype. These results suggest that the PARN-based mitochondrial mRNA regulatory system is conserved in land plants.
  • Chromatin Organization in Early Land Plants Reveals an Ancestral Association between H3K27me3, Transposons, and Constitutive Heterochromatin., Sean A Montgomery, Yasuhiro Tanizawa, Bence Galik, Nan Wang, Tasuku Ito, Takako Mochizuki, Svetlana Akimcheva, John L Bowman, Valérie Cognat, Laurence Maréchal-Drouard, Heinz Ekker, Syuan-Fei Hong, Takayuki Kohchi, Shih-Shun Lin, Li-Yu Daisy Liu, Yasukazu Nakamura, Lia R Valeeva, Eugene V Shakirov, Dorothy E Shippen, Wei-Lun Wei, Masaru Yagura, Shohei Yamaoka, Katsuyuki T Yamato, Chang Liu, Frédéric Berger, Current biology : CB, Current biology : CB, 30(4), 573 - 588, Feb. 24 2020 , Refereed
    Summary:Genome packaging by nucleosomes is a hallmark of eukaryotes. Histones and the pathways that deposit, remove, and read histone modifications are deeply conserved. Yet, we lack information regarding chromatin landscapes in extant representatives of ancestors of the main groups of eukaryotes, and our knowledge of the evolution of chromatin-related processes is limited. We used the bryophyte Marchantia polymorpha, which diverged from vascular plants circa 400 mya, to obtain a whole chromosome genome assembly and explore the chromatin landscape and three-dimensional genome organization in an early diverging land plant lineage. Based on genomic profiles of ten chromatin marks, we conclude that the relationship between active marks and gene expression is conserved across land plants. In contrast, we observed distinctive features of transposons and other repetitive sequences in Marchantia compared with flowering plants. Silenced transposons and repeats did not accumulate around centromeres. Although a large fraction of constitutive heterochromatin was marked by H3K9 methylation as in flowering plants, a significant proportion of transposons were marked by H3K27me3, which is otherwise dedicated to the transcriptional repression of protein-coding genes in flowering plants. Chromatin compartmentalization analyses of Hi-C data revealed that repressed B compartments were densely decorated with H3K27me3 but not H3K9 or DNA methylation as reported in flowering plants. We conclude that, in early plants, H3K27me3 played an essential role in heterochromatin function, suggesting an ancestral role of this mark in transposon silencing.
  • The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha., Takuma Hiwatashi, Honzhen Goh, Yukiko Yasui, Li Quan Koh, Hideyuki Takami, Masataka Kajikawa, Hiroyuki Kirita, Takehiko Kanazawa, Naoki Minamino, Taisuke Togawa, Mayuko Sato, Mayumi Wakazaki, Katsushi Yamaguchi, Shuji Shigenobu, Hidehiro Fukaki, Tetsuro Mimura, Kiminori Toyooka, Shinichiro Sawa, Katsuyuki T Yamato, Takashi Ueda, Daisuke Urano, Takayuki Kohchi, Kimitsune Ishizaki, Current biology : CB, Current biology : CB, 29(20), 3525 - 3531, Oct. 21 2019 , Refereed
    Summary:Many plants can reproduce vegetatively, producing clonal progeny from vegetative cells; however, little is known about the molecular mechanisms underlying this process. Liverwort (Marchantia polymorpha), a basal land plant, propagates asexually via gemmae, which are clonal plantlets formed in gemma cups on the dorsal side of the vegetative thallus [1]. The initial stage of gemma development involves elongation and asymmetric divisions of a specific type of epidermal cell, called a gemma initial, which forms on the floor of the gemma cup [2, 3]. To investigate the regulatory mechanism underlying gemma development, we focused on two allelic mutants in which no gemma initial formed; these mutants were named karappo, meaning "empty." We used whole-genome sequencing of both mutants and molecular genetic analysis to identify the causal gene, KARAPPO (KAR), which encodes a ROP guanine nucleotide exchange factor (RopGEF) carrying a plant-specific ROP nucleotide exchanger (PRONE) catalytic domain. In vitro GEF assays showed that the full-length KAR protein and the PRONE domain have significant GEF activity toward MpROP, the only ROP GTPase in M. polymorpha. Moreover, genetic complementation experiments showed a significant role for the N- and C-terminal variable regions in gemma development. Our investigation demonstrates an essential role for KAR/RopGEF in the initiation of plantlet development from a differentiated cell, which may involve cell-polarity formation and subsequent asymmetric cell division via activation of ROP signaling, implying a similar developmental mechanism in vegetative reproduction of various land plants.
  • Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants, Asuka Higo, Tomokazu Kawashima, Michael Borg, Mingmin Zhao, Irene López-Vidriero, Hidetoshi Sakayama, Sean A. Montgomery, Hiroyuki Sekimoto, Dieter Hackenberg, Masaki Shimamura, Tomoaki Nishiyama, Keiko Sakakibara, Yuki Tomita, Taisuke Togawa, Kan Kunimoto, Akihisa Osakabe, Yutaka Suzuki, Katsuyuki T. Yamato, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, José M. Franco-Zorrilla, David Twell, Frédéric Berger, Takashi Araki, Nature Communications, Nature Communications, 9(1), 5283, Dec. 2018 , Refereed
  • Generative Cell Specification Requires Transcription Factors Evolutionarily Conserved in Land Plants., Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, Keisuke Inoue, Misaki Saito, Keitaro Okahashi, Haonan Bao, Hiroyuki Nishida, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T Yamato, Takayuki Kohchi, Current biology : CB, Current biology : CB, 28(3), 479 - 486, Feb. 05 2018 , Refereed
    Summary:Land plants differentiate germ cells in the haploid gametophyte. In flowering plants, a generative cell is specified as a precursor that subsequently divides into two sperm cells in the developing male gametophyte, pollen. Generative cell specification requires cell-cycle control and microtubule-dependent nuclear relocation (reviewed in [1-3]). However, the generative cell fate determinant and its evolutionary origin are still unknown. In bryophytes, gametophytes produce eggs and sperm in multicellular reproductive organs called archegonia and antheridia, respectively, or collectively called gametangia. Given the monophyletic origin of land plants [4-6], evolutionarily conserved mechanisms may play key roles in these diverse reproductive processes. Here, we showed that a single member of the subfamily VIIIa of basic helix-loop-helix (bHLH) transcription factors in the liverwort Marchantia polymorpha primarily accumulated in the initial cells and controlled their development into gametangia. We then demonstrated that an Arabidopsis thaliana VIIIa bHLH transiently accumulated in the smaller daughter cell after an asymmetric division of the meiosis-derived microspore and was required for generative cell specification redundantly with its paralog. Furthermore, these A. thaliana VIIIa bHLHs were functionally replaceable by the M. polymorpha VIIIa bHLH. These findings suggest the VIIIa bHLH proteins as core regulators for reproductive development, including germ cell differentiation, since an early stage of land plant evolution.
  • Identification of a hexenal reductase that modulates the composition of green leaf volatiles, Toshiyuki Tanaka, Ayana Ikeda, Kaori Shiojiri, Rika Ozawa, Kazumi Shiki, Naoko Nagai-Kunihiro, Kenya Fujita, Koichi Sugimoto, Katsuyuki T. Yamato, Hideo Dohra, Toshiyuki Ohnishi, Takao Koeduka, Kenji Matsui, Plant Physiology, Plant Physiology, 178(2), 552 - 564, 2018 , Refereed
    Summary:, Copyright © 2018 American Society of Plant Biologists. All rights reserved. Green leaf volatiles (GLVs), including six-carbon (C6) aldehydes, alcohols, and esters, are formed when plant tissues are damaged. GLVs play roles in direct plant defense at wound sites, indirect plant defense via the attraction of herbivore predators, and plant-plant communication. GLV components provoke distinctive responses in their target recipients; therefore, the control of GLV composition is important for plants to appropriately manage stress responses. The reduction of C6-aldehydes into C6-alcohols is a key step in the control of GLV composition and also is important to avoid a toxic buildup of C6-aldehydes. However, the molecular mechanisms behind C6-aldehyde reduction remain poorly understood. In this study, we purified an Arabidopsis (Arabidopsis thaliana) NADPH-dependent cinnamaldehyde and hexenal reductase encoded by At4g37980, named here CINNAMALDEHYDE AND HEXENAL REDUCTASE (CHR). CHR T-DNA knockout mutant plants displayed a normal growth phenotype; however, we observed significant suppression of C6-alcohol production following partial mechanical wounding or herbivore infestation. Our data also showed that the parasitic wasp Cotesia vestalis was more attracted to GLVs emitted from herbivore-infested wild-type plants compared with GLVs emitted from chr plants, which corresponded with reduced C6-alcohol levels in the mutant. Moreover, chr plants were more susceptible to exogenous high-dose exposure to (Z)-3-hexenal, as indicated by their markedly lowered photosystem II activity. Our study shows that reductases play significant roles in changing GLV composition and, thus, are important in avoiding toxicity from volatile carbonyls and in the attraction of herbivore predators.
  • De novo short read assembly and functional annotation of eleocharis vivipara, a C3/C4 interconvertible sedge plant, Daijiro Harada, Katsuyuki T. Yamato, Katsura Izui, Motomu Akita, Environmental Control in Biology, Environmental Control in Biology, 56(2), 81 - 87, 2018 , Refereed
    Summary:Eleocharis vivipara is an amphibious sedge that displays C4 traits under terrestrial environments and C3 traits in submerged environments. This plant is thus potentially advantageous for screening genes indispensable to the development of C4 photosynthesis. In this study, we performed de novo transcriptome analysis of E. vivipara using its terrestrial- and submerged-type plants. By next-generation sequencing (NGS), approximately 90 and 89 million reads were yielded for the terrestrial and submerged types, respectively, and were assembled into 27,249 unigenes. Of these de novo consensus sequences, 94.5% showed similarities to database-registered sequences, and 69.4% were assigned with Gene Ontology terms. Our de novo assembled sequence data should provide a foundation for genetic analysis of the C4 photosynthetic system.
  • Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome., John L Bowman, Takayuki Kohchi, Katsuyuki T Yamato, Jerry Jenkins, Shengqiang Shu, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama, Yasukazu Nakamura, Frédéric Berger, Catherine Adam, Shiori Sugamata Aki, Felix Althoff, Takashi Araki, Mario A Arteaga-Vazquez, Sureshkumar Balasubrmanian, Kerrie Barry, Diane Bauer, Christian R Boehm, Liam Briginshaw, Juan Caballero-Perez, Bruno Catarino, Feng Chen, Shota Chiyoda, Mansi Chovatia, Kevin M Davies, Mihails Delmans, Taku Demura, Tom Dierschke, Liam Dolan, Ana E Dorantes-Acosta, D Magnus Eklund, Stevie N Florent, Eduardo Flores-Sandoval, Asao Fujiyama, Hideya Fukuzawa, Bence Galik, Daniel Grimanelli, Jane Grimwood, Ueli Grossniklaus, Takahiro Hamada, Jim Haseloff, Alexander J Hetherington, Asuka Higo, Yuki Hirakawa, Hope N Hundley, Yoko Ikeda, Keisuke Inoue, Shin-Ichiro Inoue, Sakiko Ishida, Qidong Jia, Mitsuru Kakita, Takehiko Kanazawa, Yosuke Kawai, Tomokazu Kawashima, Megan Kennedy, Keita Kinose, Toshinori Kinoshita, Yuji Kohara, Eri Koide, Kenji Komatsu, Sarah Kopischke, Minoru Kubo, Junko Kyozuka, Ulf Lagercrantz, Shih-Shun Lin, Erika Lindquist, Anna M Lipzen, Chia-Wei Lu, Efraín De Luna, Robert A Martienssen, Naoki Minamino, Masaharu Mizutani, Miya Mizutani, Nobuyoshi Mochizuki, Isabel Monte, Rebecca Mosher, Hideki Nagasaki, Hirofumi Nakagami, Satoshi Naramoto, Kazuhiko Nishitani, Misato Ohtani, Takashi Okamoto, Masaki Okumura, Jeremy Phillips, Bernardo Pollak, Anke Reinders, Moritz Rövekamp, Ryosuke Sano, Shinichiro Sawa, Marc W Schmid, Makoto Shirakawa, Roberto Solano, Alexander Spunde, Noriyuki Suetsugu, Sumio Sugano, Akifumi Sugiyama, Rui Sun, Yutaka Suzuki, Mizuki Takenaka, Daisuke Takezawa, Hirokazu Tomogane, Masayuki Tsuzuki, Takashi Ueda, Masaaki Umeda, John M Ward, Yuichiro Watanabe, Kazufumi Yazaki, Ryusuke Yokoyama, Yoshihiro Yoshitake, Izumi Yotsui, Sabine Zachgo, Jeremy Schmutz, Cell, Cell, 171(2), 287 - 304, Oct. 05 2017 , Refereed
    Summary:The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP.
  • "Fusion" in fertilization: interdisciplinary collaboration among plant and animal scientists, Katsuyuki T. Yamato, Kazuyuki Kuchitsu, JOURNAL OF PLANT RESEARCH, JOURNAL OF PLANT RESEARCH, 130(3), 419 - 421, May 2017
  • Dynamic reorganization of the endomembrane system during spermatogenesis in Marchantia polymorpha, Naoki Minamino, Takehiko Kanazawa, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda, JOURNAL OF PLANT RESEARCH, JOURNAL OF PLANT RESEARCH, 130(3), 433 - 441, May 2017 , Refereed
    Summary:The processes involved in sexual reproduction have been diversified during plant evolution. Whereas charales, bryophytes, pteridophytes, and some gymnosperms utilize motile sperm as male gametes, in other gymnosperms and angiosperms the immotile sperm cells are delivered to the egg cells through elongated pollen tubes. During formation of the motile sperms, cells undergo a dynamic morphological transformation including drastic changes in shape and the generation of locomotor architecture. The molecular mechanism involved in this process remains mostly unknown. Membrane trafficking fulfills the exchange of various proteins and lipids among single membrane-bound organelles in eukaryotic cells, contributing to various biological functions. RAB GTPases and SNARE proteins are evolutionarily conserved key machineries of membrane trafficking mechanisms, which regulate tethering and fusion of the transport vesicles to target membranes. Our observation of fluorescently tagged plasma membrane-resident SNARE proteins demonstrated that these proteins relocalize to spherical structures during the late stages in spermiogenesis. Similar changes in subcellular localization were also observed for other fluorescently tagged SNARE proteins and a RAB GTPase, which acts on other organelles including the Golgi apparatus and endosomes. Notably, a vacuolar SNARE, MpVAMP71, was localized on the membrane of the spherical structures. Electron microscopic analysis revealed that there are many degradation-related structures such as multi-vesicular bodies, autophagosomes, and autophagic bodies containing organelles. Our results indicate that the cell-autonomous degradation pathway plays a crucial role in the removal of membrane components and the cytoplasm during spermiogenesis of Marchantia polymorpha. This process differs substantially from mammalian spermatogenesis in which phagocytic removal of excess cytoplasm involves neighboring cells.
  • n-Hexanal and (Z)-3-hexenal are generated from arachidonic acid and linolenic acid by a lipoxygenase in Marchantia polymorpha L., Moataz M. Tawfik, Katsuyuki T. Yamato, Takayuki Kohchi, Takao Koeduka, Kenji Matsui, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(6), 1148 - 1155, 2017 , Refereed
    Summary:Most terrestrial plants form green leaf volatiles (GLVs), which are mainly composed of six-carbon (C6) compounds. In our effort to study the distribution of the ability of lipoxygenase (LOX) to form GLVs, we found that a liverwort, Marchantia polymorpha, formed n-hexanal and (Z)-3-hexenal. Some LOXs execute a secondary reaction to form short chain volatiles. One of the LOXs from M. polymorpha (MpLOX7) oxygenized arachidonic and -linolenic acids at almost equivalent efficiency and formed C6-aldehydes during its catalysis; these are likely formed from hydroperoxides of arachidonic and -linolenic acids, with a cleavage of the bond between carbon at the base of the hydroperoxy group and carbon of double bond, which is energetically unfavorable. These lines of evidence suggest that one of the LOXs in liverwort employs an unprecedented reaction to form C6 aldehydes as by-products of its reaction with fatty acid substrates.
  • Transcriptome and proteome analyses provide insight into laticifer's defense of Euphorbia tirucalli against pests, Sakihito Kitajima, Kenji Miura, Wataru Aoki, Katsuyuki T. Yamato, Toki Taira, Ryuta Murakami, Shunsuke Aburaya, PLANT PHYSIOLOGY AND BIOCHEMISTRY, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 108, 434 - 446, Nov. 2016 , Refereed
    Summary:The cytoplasm of laticifers, which are plant cells specialized for rubber production and defense against microbes and herbivores, is a latex. Although laticifers share common functions, the protein constituents of latexes are highly variable among plant species and even among organs. In this study, transcriptomic and proteomic analyses of Euphorbia tirucalli's (Euphorbiaceae) latex were conducted to determine the molecular basis of the laticifer's functions in this plant. The hybrid de novo assembly of Illumina mRNA-seq and expressed sequence tags obtained by Sanger's sequencing revealed 26,447 unigenes. A unigene similar to Arabidopsis embryo-specific protein 3 (AT5G62200), which is a PLAT domain-containing protein, and rubber elongation factor showed the highest expression levels. The proteome analysis, studied by liquid chromatography-mass spectrometry with the de novo assembled unigenes as the database, revealed 161 proteins in the latex, 107 of which were not detected in the stem. A gene ontology analysis indicated that the laticifer's proteome was enriched with proteins related to proteolysis, phosphatase, defense against various environmental stresses and lipid metabolisms. D-mannose-binding lectin, ricin (which lacked the N-terminal conserved ribosome-inactivating protein domain), chitinase and peroxidase were highly accumulated, as confirmed by two-dimensional polyacrylamide gel electrophoresis. Thus, the lectins and chitinase may be the major defensive proteins against pests, and the other defense-related proteins and transcripts detected in latex may work in coordination with them. Highly expressing unigenes with unknown functions are candidate novel defense- or rubber production related genes. (C) 2016 Elsevier Masson SAS. All rights reserved.
  • An Evolutionarily Conserved Plant RKD Factor Controls Germ Cell Differentiation, Satoshi Koi, Tetsuya Hisanaga, Katsutoshi Sato, Masaki Shimamura, Katsuyuki T. Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Keiji Nakajima, CURRENT BIOLOGY, CURRENT BIOLOGY, 26(13), 1775 - 1781, Jul. 2016 , Refereed
    Summary:In contrast to animals, in which the germ cell lineage is established during embryogenesis, plant germ cells are generated in reproductive organs via reprogramming of somatic cells. The factors that control germ cell differentiation and reprogramming in plants are poorly understood. Members of the RKD subfamily of plant-specific RWP-RK transcription factors have been implicated in egg cell formation in Arabidopsis based on their expression patterns and ability to cause an egg-like transcriptome upon ectopic expression [1]; however, genetic evidence of their involvement is lacking, due to possible genetic redundancy, haploid lethality, and the technical difficulty of analyzing egg cell differentiation in angiosperms. Here we analyzed the factors that govern germ cell formation in the liverwort Marchantia polymorpha. This recently revived model bryophyte has several characteristics that make it ideal for studies of germ cell formation, such as low levels of genetic redundancy, readily accessible germ cells, and the ability to propagate asexually via gemma formation [2, 3]. Our analyses revealed that MpRKD, a single RWP-RK factor closely related to angiosperm RKDs, is preferentially expressed in developing eggs and sperm precursors in M. polymorpha. Targeted disruption of MpRKD had no effect on the gross morphology of the vegetative and reproductive organs but led to striking defects in egg and sperm cell differentiation, demonstrating that MpRKD is an essential regulator of germ cell differentiation. Together with previous findings [1, 4-6], our results suggest that RKD factors are evolutionarily conserved regulators of germ cell differentiation in land plants.
  • Abscisic acid-induced gene expression in the liverwort Marchantia polymorpha is mediated by evolutionarily conserved promoter elements, Totan K. Ghosh, Midori Kaneko, Khaleda Akter, Shuhei Murai, Kenji Komatsu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Daisuke Takezawa, PHYSIOLOGIA PLANTARUM, PHYSIOLOGIA PLANTARUM, 156(4), 407 - 420, Apr. 2016 , Refereed
    Summary:Abscisic acid (ABA) is a phytohormone widely distributed among members of the land plant lineage (Embryophyta), regulating dormancy, stomata closure and tolerance to environmental stresses. In angiosperms (Magnoliophyta), ABA-induced gene expression is mediated by promoter elements such as the G-box-like ACGT-core motifs recognized by bZIP transcription factors. In contrast, the mode of regulation by ABA of gene expression in liverworts (Marchantiophyta), representing one of the earliest diverging land plant groups, has not been elucidated. In this study, we used promoters of the liverwort Marchantia polymorpha dehydrin and the wheat Em genes fused to the -glucuronidase (GUS) reporter gene to investigate ABA-induced gene expression in liverworts. Transient assays of cultured cells of Marchantia indicated that ACGT-core motifs proximal to the transcription initiation site play a role in the ABA-induced gene expression. The RY sequence recognized by B3 transcriptional regulators was also shown to be responsible for the ABA-induced gene expression. In transgenic Marchantia plants, ABA treatment elicited an increase in GUS expression in young gemmalings, which was abolished by simultaneous disruption of the ACGT-core and RY elements. ABA-induced GUS expression was less obvious in mature thalli than in young gemmalings, associated with reductions in sensitivity to exogenous ABA during gametophyte growth. In contrast, lunularic acid, which had been suggested to function as an ABA-like substance, had no effect on GUS expression. The results demonstrate the presence of ABA-specific response mechanisms mediated by conserved cis-regulatory elements in liverworts, implying that the mechanisms had been acquired in the common ancestors of embryophytes.
  • Transcriptional Framework of Male Gametogenesis in the Liverwort Marchantia polymorpha L., Asuka Higo, Masaki Niwa, Katsuyuki T. Yamato, Lixy Yamada, Hitoshi Sawada, Tomoaki Sakamoto, Tetsuya Kurata, Makoto Shirakawa, Motomu Endo, Shuji Shigenobu, Katsushi Yamaguchi, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Takashi Araki, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 57(2), 325 - 338, Feb. 2016 , Refereed
    Summary:In land plants, there are two types of male gametes: one is a non-motile sperm cell which is delivered to the egg cell by a pollen tube, and the other is a motile sperm cell with flagella. The molecular mechanism underlying the sexual reproduction with the egg and pollen-delivered sperm cell is well understood from studies using model plants such as Arabidopsis and rice. On the other hand, the sexual reproduction with motile sperm has remained poorly characterized, due to the lack of suitable models. Marchantia polymorpha L. is a model basal land plant with sexual reproduction involving an egg cell and bi-flagellated motile sperm. To understand the differentiation process of plant motile sperm, we analyzed the gene expression profile of developing antheridia of M. polymorpha. We performed RNA-sequencing experiments and compared transcript profiles of the male sexual organ (antheridiophore and antheridium contained therein), female sexual organ (archegoniophore) and a vegetative organ (thallus). Transcriptome analysis showed that the antheridium expresses nearly half of the protein-coding genes predicted in the genome, but it also has unique features. The antheridium transcriptome shares some common features with male gamete transcriptomes of angiosperms and animals, and homologs of genes involved in male gamete formation and function in angiosperms and animals were identified. In addition, we showed that some of them had distinct expression patterns in the spermatogenous tissue of developing antheridia. This study provides a transcriptional framework on which to study the molecular mechanism of plant motile sperm development in M. polymorpha as a model.
  • Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses, Pin-Chun Lin, Chia-Wei Lu, Bing-Nan Shen, Guan-Zong Lee, John L. Bowman, Mario A. Arteaga-Vazquez, Li-Yu Daisy Liu, Syuan-Fei Hong, Chu-Fang Lo, Gong-Min Su, Takayuki Kohchi, Kimitsune Ishizaki, Sabine Zachgo, Felix Althoff, Mizuki Takenaka, Katsuyuki T. Yamato, Shih-Shun Lin, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 57(2), 339 - 358, Feb. 2016 , Refereed
    Summary:Bryophytes (liverworts, hornworts and mosses) comprise the three earliest diverging lineages of land plants (embryophytes). Marchantia polymorpha, a complex thalloid Marchantiopsida liverwort that has been developed into a model genetic system, occupies a key phylogenetic position. Therefore, M. polymorpha is useful in studies aiming to elucidate the evolution of gene regulation mechanisms in plants. In this study, we used computational, transcriptomic, small RNA and degradome analyses to characterize microRNA (miRNA)-mediated pathways of gene regulation in M. polymorpha. The data have been integrated into the open access ContigViews-miRNA platform for further reference. In addition to core components of the miRNA pathway, 129 unique miRNA sequences, 11 of which could be classified into seven miRNA families that are conserved in embryophytes (miR166a, miR390, miR529c, miR171-3p, miR408a, miR160 and miR319a), were identified. A combination of computational and degradome analyses allowed us to identify and experimentally validate 249 targets. In some cases, the target genes are orthologous to those of other embryophytes, but in other cases, the conserved miRNAs target either paralogs or members of different gene families. In addition, the newly discovered Mpo-miR11707.1 and Mpo-miR11707.2 are generated from a common precursor and target MpARGONAUTE1 (LW1759). Two other newly discovered miRNAs, Mpo-miR11687.1 and Mpo-miR11681.1, target the MADS-box transcription factors MpMADS1 and MpMADS2, respectively. Interestingly, one of the pentatricopeptide repeat (PPR) gene family members, MpPPR_66 (LW9825), the protein products of which are generally involved in various steps of RNA metabolism, has a long stem-loop transcript that can generate Mpo-miR11692.1 to autoregulate MpPPR_66 (LW9825) mRNA. This study provides a foundation for further investigations of the RNA-mediated silencing mechanism in M. polymorpha as well as of the evolution of this gene silencing pathway in embryophytes.
  • The Naming of Names: Guidelines for Gene Nomenclature in Marchantia., John L Bowman, Takashi Araki, Mario A Arteaga-Vazquez, Frederic Berger, Liam Dolan, Jim Haseloff, Kimitsune Ishizaki, Junko Kyozuka, Shih-Shun Lin, Hideki Nagasaki, Hirofumi Nakagami, Keiji Nakajima, Yasukazu Nakamura, Kyoko Ohashi-Ito, Shinichiro Sawa, Masaki Shimamura, Roberto Solano, Hirokazu Tsukaya, Takashi Ueda, Yuichiro Watanabe, Katsuyuki T Yamato, Sabine Zachgo, Takayuki Kohchi, Plant & cell physiology, Plant & cell physiology, 57(2), 257 - 61, Feb. 2016 , Refereed
    Summary:While Marchantia polymorpha has been utilized as a model system to investigate fundamental biological questions for over almost two centuries, there is renewed interest in M. polymorpha as a model genetic organism in the genomics era. Here we outline community guidelines for M. polymorpha gene and transgene nomenclature, and we anticipate that these guidelines will promote consistency and reduce both redundancy and confusion in the scientific literature.
  • Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L., Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 57(2), 300 - 306, Feb. 2016 , Refereed
    Summary:The liverwort Marchantia polymorpha L. is one of the key model plants in evo-devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown M. polymorpha gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at -80A degrees C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a -80A degrees C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for M. polymorpha should increase their availability in the research community.
  • SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery, Takehiko Kanazawa, Atsuko Era, Naoki Minamino, Yu Shikano, Masaru Fujimoto, Tomohiro Uemura, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Tomoaki Nishiyama, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 57(2), 307 - 324, Feb. 2016 , Refereed
    Summary:The membrane trafficking pathway has been diversified in a specific way for each eukaryotic lineage, probably to fulfill specific functions in the organisms. In green plants, comparative genomics has supported the possibility that terrestrialization and/or multicellularization could be associated with the elaboration and diversification of membrane trafficking pathways, which have been accomplished by an expansion of the numbers of genes required for machinery components of membrane trafficking, including soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. However, information regarding membrane trafficking pathways in basal land plant lineages remains limited. In the present study, we conducted extensive analyses of SNARE molecules, which mediate membrane fusion between target membranes and transport vesicles or donor organelles, in the liverwort, Marchantia polymorpha. The M. polymorpha genome contained at least 34 genes for 36 SNARE proteins, comprising fundamental sets of SNARE proteins that are shared among land plant lineages with low degrees of redundancy. We examined the subcellular distribution of a major portion of these SNARE proteins by expressing Citrine-tagged SNARE proteins in M. polymorpha, and the results showed that some of the SNARE proteins were targeted to different compartments from their orthologous products in Arabidopsis thaliana. For example, MpSYP12B was localized to the surface of the oil body, which is a unique organelle in liverworts. Furthermore, we identified three VAMP72 members with distinctive structural characteristics, whose N-terminal extensions contain consensus sequences for N-myristoylation. These results suggest that M. polymorpha has acquired unique membrane trafficking pathways associated with newly acquired machinery components during evolution.
  • Molecular Genetic Tools and Techniques for Marchantia polymorpha Research, Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 57(2), 262 - 270, Feb. 2016 , Refereed
    Summary:Liverworts occupy a basal position in the evolution of land plants, and are a key group to address a wide variety of questions in plant biology. Marchantia polymorpha is a common, easily cultivated, dioecious liverwort species, and is emerging as an experimental model organism. The haploid gametophytic generation dominates the diploid sporophytic generation in its life cycle. Genetically homogeneous lines in the gametophyte generation can be established easily and propagated through asexual reproduction, which aids genetic and biochemical experiments. Owing to its dioecy, male and female sexual organs are formed in separate individuals, which enables crossing in a fully controlled manner. Reproductive growth can be induced at the desired times under laboratory conditions, which helps genetic analysis. The developmental process from a single-celled spore to a multicellular body can be observed directly in detail. As a model organism, molecular techniques for M. polymorpha are well developed; for example, simple and efficient protocols of Agrobacterium-mediated transformation have been established. Based on them, various strategies for molecular genetics, such as introduction of reporter constructs, overexpression, gene silencing and targeted gene modification, are available. Herein, we describe the technologies and resources for reverse and forward genetics in M. polymorpha, which offer an excellent experimental platform to study the evolution and diversity of regulatory systems in land plants.
  • Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha), Hirotomo Kihara, Maya Tanaka, Katsuyuki T. Yamato, Akira Horibata, Atsushi Yamada, Sayaka Kita, Kimitsune Ishizaki, Masataka Kajikawa, Hideya Fukuzawa, Takayuki Kohchi, Yoshihiko Akakabe, Kenji Matsui, PHYTOCHEMISTRY, PHYTOCHEMISTRY, 107, 42 - 49, Nov. 2014 , Refereed
    Summary:Eight-carbon (C8) volatiles, such as 1-octen-3-ol, octan-3-one, and octan-3-ol, are ubiquitously found among fungi and bryophytes. In this study, it was found that the thalli of the common liverwort Marchantia polymorpha, a model plant species, emitted high amounts of C8 volatiles mainly consisting of (R)-1-octen-3-ol and octan-3-one upon mechanical wounding. The induction of emission took place within 40 min. In intact thalli, 1-octen-3-yl acetate was the predominant C8 volatile while tissue disruption resulted in conversion of the acetate to 1-octen-3-ol. This conversion was carried out by an esterase showing stereospecificity to (R)-1-octen-3-yl acetate. From the transgenic line of M. polymorpha (des6(KO)) lacking arachidonic acid and eicosapentaenoic acid, formation of C8 volatiles was only minimally observed, which indicated that arachidonic and/or eicosapentaenoic acids were essential to form C8 volatiles in M. polymorpha. When des6(KO) thalli were exposed to the vapor of 1-octen-3-ol, they absorbed the alcohol and converted it into 1-octen-3-yl acetate and octan-3-one. Therefore, this implied that 1-octen-3-ol was the primary C8 product formed from arachidonic acid, and further metabolism involving acetylation and oxidoreduction occurred to diversify the C8 products. Octan-3-one was only minimally formed from completely disrupted thalli, while it was formed as the most abundant product in partially disrupted thalli. Therefore, it is assumed that the remaining intact tissues were involved in the conversion of 1-octen-3-ol to octan-3-one in the partially disrupted thalli. The conversion was partly promoted by addition of NAD(P)H into the completely disrupted tissues, suggesting an NAD(P)H-dependent oxidoreductase was involved in the conversion. (C) 2014 Elsevier Ltd. All rights reserved.
  • Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha, Aino Komatsu, Mika Terai, Kimitsune Ishizaki, Noriyuki Suetsugu, Hidenori Tsuboi, Ryuichi Nishihama, Katsuyuki T. Yamato, Masamitsu Wada, Takayuki Kohchi, PLANT PHYSIOLOGY, PLANT PHYSIOLOGY, 166(1), 411 - U598, Sep. 2014 , Refereed
    Summary:Blue-light-induced chloroplast photorelocation movement is observed in most land plants. Chloroplasts move toward weak-light-irradiated areas to efficiently absorb light (the accumulation response) and escape from strong-light-irradiated areas to avoid photodamage (the avoidance response). The plant-specific kinase phototropin (phot) is the blue-light receptor for chloroplast movements. Although the molecular mechanisms for chloroplast photorelocation movement have been analyzed, the overall aspects of signal transduction common to land plants are still unknown. Here, we show that the liverwort Marchantia polymorpha exhibits the accumulation and avoidance responses exclusively induced by blue light as well as specific chloroplast positioning in the dark. Moreover, in silico and Southern-blot analyses revealed that the M. polymorpha genome encodes a single PHOT gene, MpPHOT, and its knockout line displayed none of the chloroplast photorelocation movements, indicating that the sole MpPHOT gene mediates all types of movement. Mpphot was localized on the plasma membrane and exhibited blue-light-dependent autophosphorylation both in vitro and in vivo. Heterologous expression of MpPHOT rescued the defects in chloroplast movement of phot mutants in the fern Adiantum capillus-veneris and the seed plant Arabidopsis (Arabidopsis thaliana). These results indicate that Mpphot possesses evolutionarily conserved regulatory activities for chloroplast photorelocation movement. M. polymorpha offers a simple and versatile platform for analyzing the fundamental processes of phototropin-mediated chloroplast photorelocation movement common to land plants.
  • Physcomitrella patens Has Kinase-LRR R Gene Homologs and Interacting Proteins, Yusuke Tanigaki, Kenji Ito, Yoshiyuki Obuchi, Akiko Kosaka, Katsuyuki T. Yamato, Masahiro Okanami, Mikko T. Lehtonen, Jari P. T. Valkonen, Motomu Akita, PLOS ONE, PLOS ONE, 9(4), e95118, Apr. 2014 , Refereed
    Summary:Plant disease resistance gene (R gene)-like sequences were screened from the Physcomitrella patens genome. We found 603 kinase-like, 475 Nucleotide Binding Site (NBS)-like and 8594 Leucine Rich Repeat (LRR)-like sequences by homology searching using the respective domains of PpC24 (Accession No. BAD38895), which is a candidate kinase-NBS-LRR (kinase-NL) type R-like gene, as a reference. The positions of these domains in the genome were compared and 17 kinase-NLs were predicted. We also found four TIR-NBS-LRR (TIR-NL) sequences with homology to Arabidopsis TIR-NL (NM_001125847), but three out of the four TIR-NLs had tetratricopeptide repeats or a zinc finger domain in their predicted C-terminus. We also searched for kinase-LRR (KLR) type sequences by homology with rice OsXa21 and Arabidopsis thaliana FLS2. As a result, 16 KLRs with similarity to OsXa21 were found. In phylogenetic analysis of these 16 KLRs, PpKLR36, PpKLR39, PpKLR40, and PpKLR43 formed a cluster with OsXa21. These four PpKLRs had deduced transmembrane domain sequences and expression of all four was confirmed. We also found 14 homologs of rice OsXB3, which is known to interact with OsXa21 and is involved in signal transduction. Protein-protein interaction was observed between the four PpKLRs and at least two of the XB3 homologs in Y2H analysis.
  • Co-option of a photoperiodic growth-phase transition system during land plant evolution, Akane Kubota, Shogo Kita, Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi, NATURE COMMUNICATIONS, NATURE COMMUNICATIONS, 5, 3668, Apr. 2014 , Refereed
    Summary:Photoperiodic control of the phase transition from vegetative to reproductive growth is critical for land plants. The GIGANTEA (GI) and FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1) protein complex controls this process in angiosperms. However, little is known about how plants evolved this regulatory system. Here, we report that orthologues of GI and FKF1 are present in a basal plant, the liverwort Marchantia polymorpha, and describe the molecular interaction between their products. Knockout of either the GI or FKF1 orthologue completely abolishes the long-day-dependent growth-phase transition in M. polymorpha. Overexpression of either gene promotes growth-phase transition, even under short-day conditions. Introduction of the GI orthologue partially rescues the late-flowering phenotype of the Arabidopsis thaliana gi mutant. Our findings suggest that plants had already acquired the GI-FKF1 system to regulate growth-phase transition when they colonized land, and that this system was co-opted from gametophyte to sporophyte generation during evolution.
  • Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha, Kimitsune Ishizaki, Maiko Nonomura, Hirotaka Kato, Katsuyuki T. Yamato, Takayuki Kohchi, JOURNAL OF PLANT RESEARCH, JOURNAL OF PLANT RESEARCH, 125(5), 643 - 651, Sep. 2012 , Refereed
    Summary:The phytohormone auxin plays a pivotal role in various developmental aspects in land plants. However, little is known of the auxin response and distribution in non-vascular plants. In this study, we made transgenic plants of the liverwort Marchantia polymorpha which express the uidA (GUS) reporter gene under control of the soybean auxin-inducible promoter, ProGH3, and used it to indirectly monitor auxin-mediated transcriptional activation in planta. Transgenic plants carrying ProGH3:GUS showed GUS activity in an auxin-dependent manner. Histochemical GUS staining was observed at the bottom of gemma cups in the process of vegetative propagation. Significant GUS activity was also detected around the gametophyte-sporophyte junction as well as the developing sporophyte after fertilization. These results suggest that the activity of auxin is crucial in both gametophyte and sporophyte development in M. polymorpha, and that the mechanism for auxin-mediated transcriptional activation had already been established when plants emerged on the terrestrial environment.
  • Comparative study of gene expression and major proteins' function of laticifers in lignified and unlignified organs of mulberry, Sakihito Kitajima, Toki Taira, Kenji Oda, Katsuyuki T. Yamato, Yoshihiro Inukai, Yusuke Hori, PLANTA, PLANTA, 235(3), 589 - 601, Mar. 2012 , Refereed
    Summary:A laticifer is a cell involved in plant defense against biotic stresses such as herbivores and microorganisms; however, its gene expression is poorly understood. We compared protein accumulation and transcriptomes among laticifers of lignified and unlignified organs of mulberry (Morus alba), which has a non-articulated, branched type of laticifer. LA-a (equivalent to MLX56) and its homolog LA-b (insecticidal chitinase-like proteins containing two chitin-binding domains) were major proteins in laticifers of unlignified organs, and another protein (LA-c) was a major protein in laticifers of lignified organs. Purification, cDNA cloning, and bioassay of LA-c revealed that LA-c was an acidic class I chitinase having antifungal but not insecticidal activity. Comparative mRNA-Seq analysis using a GS-FLX revealed transcripts of other possible defense-related proteins. Jacalin-like lectin, galacturonase-inhibitor, and pathogenesis-related proteins were also abundant; however, the relative amounts differed among laticifers of lignified and unlignified organs. The results suggest a discontinuous laticifer network in planta and adaptation to different potential enemies among these organs.
  • ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus, Naoko Minamisawa, Mayuko Sato, Kiu-Hyung Cho, Hanako Ueno, Katsuaki Takechi, Masataka Kajikawa, Katsuyuki T. Yamato, Kanji Ohyama, Kiminori Toyooka, Gyung-Tae Kim, Gorou Horiguchi, Hiroyoshi Takano, Takashi Ueda, Hirokazu Tsukaya, PLANT JOURNAL, PLANT JOURNAL, 68(5), 788 - 799, Dec. 2011 , Refereed
    Summary:CtBP/BARS is a unique protein family in having quite diversified cellular functions, intercellular localizations, and developmental roles. ANGUSTIFOLIA (AN) is the sole homolog of CtBP/BARS from Arabidopsis thaliana, although it has plant AN-specific motifs and a long C-terminus. Previous studies suggested that AN would function in the nucleus as a transcriptional co-repressor, as CtBPs function in animals; however, precise verification has been lacking. In this paper, we isolated a homologous gene (MAN) of AN from liverwort, Marchantia polymorpha. Transformation of the Arabidopsis an-1 mutant with 35S-driven MAN completely complemented the an-1 phenotype, although it lacks the putative nuclear localization signal (NLS) that exists in AN proteins isolated from other plant species. We constructed several plasmids for expressing modified ANs with amino acid substitutions in known motifs. The results clearly indicated that modified AN with mutations in the putative NLS-like domain could complement the an-1 phenotype. Therefore, we re-examined localization of AN using several techniques. Our results demonstrated that AN localizes on punctuate structures around the Golgi, partially overlapping with a trans-Golgi network resident, which highlighted an unexpected link between leaf development and membrane trafficking. We should reconsider the roles and evolutionary traits of AN based on these findings.
  • Characterization of the Lipid Accumulation in a New Microalgal Species, Pseudochoricystis ellipsoidea (Trebouxiophyceae), Akira Satoh, Misako Kato, Katsuyuki Yamato, Mizuki Ishibashi, Hiroshi Sekiguchi, Norihide Kurano, Shigetoh Miyachi, Nihon Enerugi Gakkaishi/Journal of the Japan Institute of Energy, Nihon Enerugi Gakkaishi/Journal of the Japan Institute of Energy, 89(9), 909 - 913, Sep. 2010 , Refereed
    Summary:The use of fast-growing oleaginous algal strains is indispensable to achieve low-cost production system for microalgal biodiesel. We therefore previously isolated a new algal species within new genus " Pseudochoricystisas" (invalid name, class Trebouxiophyceae), "Pseudochoricystis eHipsoidea" (MBIC11204), which possesses oil vesicles stainable with a selective fluorescent dye for intracellular lipid droplets, Nile Red (Sekiguchi et al., 12th Annu. Meet. Jpn. Microbiol. Cult. Coll. 2005). In the present study we attempted to investigate growth and lipid accumulation of this new isolate. The maximum growth rate of P. ellipsoidea was 3.46 g dry weight 1-1 day-1. Nile Red fluorescence reached maximum intensity within 5-10 days after transferring P. ellipsoidea cells to nitrogen starvation conditions in the light, but not in the dark. Total lipid content made up 32% of normal-grown (+N) and 26% of nitrogen-starved (-N, 8 days) dry weight algal cells, and the hydrocarbon fraction was more than 10 times higher in -N cells. Fatty acid composition changes and an increase in triglycerides to 82% of total lipid were also observed with nitrogen starvation. These results suggest that P. ellipsoidea is a fast-growing oleaginous algal strain in which hydrocarbons and triglycerides can be produced photoautotrophically up to 30% of the dried biomass.
  • Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha, Ken Tougane, Kenji Komatsu, Salma Begum Bhyan, Yoichi Sakata, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Daisuke Takezawa, PLANT PHYSIOLOGY, PLANT PHYSIOLOGY, 152(3), 1529 - 1543, Mar. 2010 , Refereed
    Summary:Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the beta-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.
  • Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha, Atsuko Era, Motoki Tominaga, Kazuo Ebine, Chie Awai, Chieko Saito, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 50(6), 1041 - 1048, Jun. 2009 , Refereed
    Summary:Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a LifeactVenus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used LifeactVenus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages.
  • Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L., Hidenobu Uchida, Hirofumi Yamashita, Masataka Kajikawa, Kiyoshi Ohyama, Osamu Nakayachi, Ryuji Sugiyama, Katsuyuki T. Yamato, Toshiya Muranaka, Hideya Fukuzawa, Miho Takemura, Kanji Ohyama, PLANTA, PLANTA, 229(6), 1243 - 1252, May 2009 , Refereed
    Summary:Euphorbia tirucalli L., which is also known as a petroleum plant, produces a large amount of phytosterols and triterpenes. During their biosynthesis, squalene synthase converts two molecules of the hydrophilic substrate farnesyl diphosphate into a hydrophobic product, squalene. An E. tirucalli cDNA clone of a putative squalene synthase gene (EtSS) was isolated by RT-PCR followed by 5'- and 3'-RACE. The restriction fragment polymorphisms revealed by Southern blot analysis suggest that EtSS is a single copy gene. The glycine at the 287th residue from the N-terminal end of domain C has replaced alanine, which is conserved among all the other SS sequences deposited in the Genbank database. The N-terminal 380 residues of the hydrophilic sequence was expressed as a peptide-tagged protein in E. coli, and the resultant bacterial crude extract was incubated with farnesyl diphosphate and NADPH. GC-MS analysis showed that squalene was detected in the in vitro reaction mixture. E. tirucalli transgenic callus lines, in which EtSS was overexpressed, accumulated increased amounts of phytosterols as compared with that of wild type callus. RT-PCR analysis of wild type E. tirucalli plants revealed that the EtSS transcript accumulated in almost equal amounts in the stems and the leaves with a stalk, while a lower amount was detected in the roots. In situ hybridization analysis revealed that prominent antisense-probe signal was detected in the cambia within bundle sheathes. These results indicate that EtSS functions prominently in cambia, which are located adjacent to conductive tubes, and that this gene plays important roles in phytosterol accumulation in petroleum plants.
  • Gene content, organization and molecular evolution of plant organellar genomes and sex chromosomes - Insights from the case of the liverwort Marchantia polymorpha, Kanji Ohyama, Miho Takemura, Kenji Oda, Hideya Fukuzawa, Takayuki Kohchi, Sigeki Nakayama, Kimitsune Ishizaki, Masaki Fujisawa, Katsuyuki Yamato, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 85(3), 108 - 124, Mar. 2009 , Refereed
    Summary:The complete nucleotide sequence of chloroplast DNA (121;025 base pairs, bp) from a liverwort, Marchantia polymorpha, has made clear the entire gene organization of the chloroplast genome. Quite a few genes encoding components of photosynthesis and protein synthesis machinery have been identified by comparative computer analysis. We also determined the complete nucleotide sequence of the liverwort mitochondrial DNA and deduced 96 possible genes in the sequence of 186,608 bp. The complete chloroplast geuome encodes twenty introns (19 group II and I group I) in 18 different, genes. One of the chloroplast group II introns separates a ribosomal protein gene in a trans-position. The mitochondrial genome contains thirty-two introns (25 group II and 7 group I) in the coding regions of 17 genes. From the evolutionary point of view, we describe the origin of organellar introns and give evidence for vertical and horizontal intron transfers and their intragenomic propagation. Furthermore, we describe the gene organization of the Y chromosome in the dioecious liverwort M. polymorpha, the first detailed view of a Y chromosome in a haploid organism. On the 10 megabase (Mb) Y chromosome, 64 genes are identified, 14 of which are detected only in the male genome. These 14 genes are expressed in reproductive organs but not in vegetative thalli, suggesting their participation in male reproductive functions. These findings indicate that the Y and X chromosomes share the same ancestral autosome and support the prediction that in a haploid organism essential genes on sex chromosomes are more likely to persist than in a diploid organism.
  • Direct transformation of the liverwort Marchantia polymorpha L. by particle bombardment using immature thalli developing from spores, Shota Chiyoda, Kimitsune Ishizaki, Hideo Kataoka, Katsuyuki T. Yamato, Takayuki Kohchi, PLANT CELL REPORTS, PLANT CELL REPORTS, 27(9), 1467 - 1473, Sep. 2008 , Refereed
    Summary:The liverwort, Marchantia polymorpha L., belongs to a group of basal land plants and is an emerging model for plant biology. We established a procedure to prepare sporangia of M. polymorpha under laboratory conditions by promoting its transition to reproductive development by far-red light irradiation. Here we report an improved direct transformation system of M. polymorpha using immature thalli developing from spores. Hygromycin-resistant transformants were obtained on selective media by transformation with a plasmid carrying the hygromycin-phosphotransferase gene (hpt) conferring hygromycin resistance in 4 weeks. The aminoglycoside-3 ''-adenyltransferase gene (aadA) conferring spectinomycin resistance was also successfully used as an additional selectable marker for nuclear transformation of M. polymorpha. The availability of the aadA gene in addition to the hpt gene should make M. polymorpha a versatile host for genetic manipulation. DNA gel-blot analyses indicated that transformed thalli carried a variable number of copies of the transgene integrated into the genome. Although the previous system using thalli grown from gemmae required a two-step selection in liquid and solid media for 8 weeks, the system reported here using thalli developing from spores allows generation of transformants in half the time by direct selection on solid media, facilitating genetic analyses in this model plant.
  • Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology, Kimitsune Ishizaki, Shota Chiyoda, Katsuyuki T. Yamato, Takayuki Kohchi, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 49(7), 1084 - 1091, Jul. 2008 , Refereed
    Summary:Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that contains a reporter, the beta-glucuronidase (GUS) gene with an intron, and a selection marker, the hygromycin phosphotransferase (hpt) gene. In this system, individual gemmae, which arise asexually from single initial cells, were analyzed as isogenic transformants. GUS activity staining showed that all hygromycin-resistant plants examined expressed the GUS transgene in planta. DNA analyses verified random integration of 1-5 copies of the intact T-DNA between the right and the left borders into the M. polymorpha genome. The efficient and rapid Agrobacterium-mediated transformation of M. polymorpha should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.
  • Production of arachidonic and eicosapentaenoic acids in plants using bryophyte fatty acid Delta 6-Desaturase, Delta 6-Elongase, and Delta 5-Desaturase genes, Masataka Kajikawa, Keisuke Matsui, Misa Ochiai, Yoshikazu Tanaka, Yoichi Kita, Masao Ishimoto, Yoshito Kohzu, Shin-Ichiro Shoji, Katsuyuki T. Yamato, Kanji Ohyama, Hideya Fukuzawa, Takayuki Kohchi, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72(2), 435 - 444, Feb. 2008 , Refereed
    Summary:The liverwort Marchantia polymorpha L. synthesizes arachidonic (ARA) and eicosapentaenoic acids (EPA) from linoleic and alpha-linolenic acids respectively by a series of reactions catalyzed by Delta 6-desaturase, Delta 6-elongase, and Delta 5-desaturase. Overexpression of the M. polymorpha genes encoding these enzymes in transgenic M. polymorpha plants resulted in 3- and 2-fold accumulation of ARA and EPA respectively, as compared to those in the wild type. When these three genes were introduced and co-expressed in tobacco plants, in which long-chain polyunsaturated fatty acids (LCPU-FAs) are not native cellular components, ARA and EPA represented up to 15.5% and 4.9% respectively of the total fatty acid in the leaves. Similarly in soybean, C20-LCPUFAs represented up to 19.5% of the total fatty acids in the seeds. These results suggest that M. polymorpha can provide genes crucial to the production of C20-LCPUFAs in transgenic plants.
  • Gene organization of the liverwort Y chromosome reveals distinct sex chromosome evolution in a haploid system, Katsuyuki T. Yamato, Kimitsune Ishizaki, Masaki Fujisawa, Sachiko Okada, Shigeki Nakayama, Mariko Fujishita, Hiroki Bando, Kohei Yodoya, Kiwako Hayashi, Tomoyuki Bando, Akiko Hasumi, Tomohisa Nishio, Ryoko Sakata, Masayuki Yamamoto, Arata Yamaki, Masataka Kajikawa, Takashi Yamano, Taku Nishide, Seung-Hyuk Choi, Yuu Shimizu-Ueda, Tsutomu Hanajiri, Megumi Sakaida, Kaoru Kono, Mizuki Takenaka, Shohei Yamaoka, Chiaki Kuriyama, Yoshito Kohzu, Hiroyuki Nishida, Axel Brennicke, Tadasu Shin-i, Yuji Kohara, Takayuki Kohchi, Hideya Fukuzawa, Kanji Ohyama, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(15), 6472 - 6477, Apr. 2007 , Refereed
    Summary:Y chromosomes are different from other chromosomes because of a lack of recombination. Until now, complete sequence information of Y chromosomes has been available only for some primates, although considerable information is available for other organisms, e.g., several species of Drosophila. Here, we report the gene organization of the Y chromosome in the dioecious liverwort Marchantia polymorpha and provide a detailed view of a Y chromosome in a haploid organism. On the 10-Mb Y chromosome, 64 genes are identified, 14 of which are detected only in the male genome and are expressed in reproductive organs but not in vegetative thalli, suggesting their participation in male reproductive functions. Another 40 genes on the Y chromosome are expressed in thalli and male sexual organs. At least six of these genes have diverged X-linked counterparts that are in turn expressed in thalli and sexual organs in female plants, suggesting that these X-and Y-linked genes have essential cellular functions. These findings indicate that the Y and X chromosomes share the same ancestral autosome and support the prediction that in a haploid organism essential genes on sex chromosomes are more likely to persist than in a diploid organism.
  • Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells, Shota Chiyoda, Philip J. Linley, Katsuyuki T. Yamato, Hideya Fukuzawa, Akiho Yokota, Takayuki Kohchi, TRANSGENIC RESEARCH, TRANSGENIC RESEARCH, 16(1), 41 - 49, Feb. 2007 , Refereed
    Summary:We have established a simple and efficient plastid transformation system for liverwort, Marchantia polymorpha L., suspension-culture cells, which are homogenous, chloroplast-rich and rapidly growing. Plasmid pCS31 was constructed to integrate an aadA expression cassette for spectinomycin-resistance into the trnI-trnA intergenic region of the liverwort plastid DNA by homologous recombination. Liverwort suspension-culture cells were bombarded with pCS31-coated gold projectiles and selected on a medium containing spectinomycin. Plastid transformants were reproducibly isolated from the obtained spectinomycin-resistant calli. Selection on a sucrose-free medium greatly improved the efficiency of selection of plastid transformants. Homoplasmic plastid transformant lines were established by successive subculturing for 14 weeks or longer on the spectinomycin-containing medium. The plastid transformation system of liverwort suspension-culture cells should facilitate the investigation of the fundamental genetic systems of plastid DNA, such as replication.
  • A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by omega 13 desaturation in methylotrophic yeast and tobacco, M Kajikawa, KT Yamato, Y Kohzu, S Shoji, K Matsui, Y Tanaka, Y Sakai, H Fukuzawa, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 47(1), 64 - 73, Jan. 2006 , Refereed
    Summary:Pinolenic acid (PA; 18:3 Delta(5,9,12)) and coniferonic acid (CA; 18:4 Delta(5,9,12,15)) are Delta(5)-unsaturated bis-methylene-interrupted fatty acids (Delta(5)-UBIFAs) commonly found in pine seed oil. They are assumed to be synthesized from linoleic acid (LA; 18:2 Delta(9,12)) and alpha-linolenic acid (ALA; 18:3 Delta(9,12,15)), respectively, by Delta(5)-desaturation. A unicellular green microalga Chlamydomonas reinhardtii also accumulates PA and CA in a betain lipid. The expressed sequence tag (EST) resource of C. reinhardtii led to the isolation of a cDNA clone that encoded a putative fatty acid desaturase named as CrDES containing a cytochrome b5 domain at the N-terminus. When the coding sequence was expressed heterologously in the methylotrophic yeast Pichia pastoris, PA and CA were newly detected and comparable amounts of LA and ALA were reduced, demonstrating that CrDES has Delta(5)-desaturase activity for both LA and ALA. CrDES expressed in the yeast showed Delta(5)-desaturase activity on 18:1 Delta(9) but not 18: 1 Delta(11). Unexpectedly, CrDES also showed Delta(5)-desaturase activity on 20:2 Delta(11,14) and 20:3 Delta(11,14,17) to produce 20:3 Delta(7,11,14) and 20:4 Delta(7,11,14,17), respectively. Since both the Delta(5) bond in C18 and the Delta(7) bond in C20 fatty acids are 'omega 13' double bonds, these results indicate that CrDES has omega 13 desaturase activity for omega 9 unsaturated C18/C20 fatty acids, in contrast to the previously reported front-end desaturases. In order to evaluate the activity of CrDES in higher plants, transgenic tobacco plants expressing CrDES were created. PA and CA accumulated in the leaves of transgenic plants. The highest combined yield of PA and CA was 44.7% of total fatty acids, suggesting that PA and CA can be produced in higher plants on a large scale.
  • Isolation and functional characterization of fatty acid Delta 5-elongase gene from the liverwort Marchantia polymorpha L., M Kajikawa, KT Yamato, Y Sakai, H Fukuzawa, K Ohyama, T Kohchi, FEBS LETTERS, FEBS LETTERS, 580(1), 149 - 154, Jan. 2006 , Refereed
    Summary:Bryophyte Marchantia polymorpha L. produces C22 very-long-chain polyunsaturated fatty acid (VLCPUFA). Thus far, no enzyme that mediates elongation of C20 VLCPUFAs has been identified in land plants. Here, we report the isolation and characterization of the gene MpELO2, which encodes an ELO-like fatty acid elongase in M. polymorpha. Heterologous expression in yeast demonstrated that MpELO2 encodes Delta 5-elongase, which mediates elongation of arachidonic (20:4) and eicosapentaenoic acids (20:5). Phylogenetic and gene structural analysis indicated that the MpELO2 gene is closely related to bryophyte Delta 6-elongase genes for C18 fatty acid elongation and diverged from them by local gene duplication. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
  • Cloning and characterization of a cDNA encoding beta-amyrin synthase from petroleum plant Euphorbia tirucalli L., M Kajikawa, KT Yamato, H Fukuzawa, Y Sakai, H Uchida, K Ohyama, PHYTOCHEMISTRY, PHYTOCHEMISTRY, 66(15), 1759 - 1766, Aug. 2005 , Refereed
    Summary:Euphorbia tirucalli L., known as the petroleum plant, produces a large amount of triterpenes, such as beta-amyrin. Degenerate RTPCR based on the sequences conserved among known beta-amyrin synthases led to cloning of a putative triterpene synthase cDNA, EtAS, from leaves of E tirucalli. The deduced amino acid sequence of the EtAS cDNA showed the highest identity of 82% to the Panax ginseng P-amyrin synthase. Heterologous expression of the EtAS ORF in the methylotrophic yeast, Pichia pastoris, resulted in production of beta-amyrin, revealing that the EtAS cDNA codes for a P-amyrin synthase. This is the first report of a gene involved in the triterpene synthetic pathway from Euphorbiaceae plants. (c) 2005 Elsevier Ltd. All rights reserved.
  • Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii., Kenji Miura, Takashi Yamano, Satoshi Yoshioka, Tsutomu Kohinata, Yoshihiro Inoue, Fumiya Taniguchi, Erika Asamizu, Yasukazu Nakamura, Satoshi Tabata, Katsuyuki T Yamato, Kanji Ohyama, Hideya Fukuzawa, Plant physiology, Plant physiology, 135(3), 1595 - 607, Jul. 2004 , Refereed
    Summary:Photosynthetic acclimation to CO2-limiting stress is associated with control of genetic and physiological responses through a signal transduction pathway, followed by integrated monitoring of the environmental changes. Although several CO2-responsive genes have been previously isolated, genome-wide analysis has not been applied to the isolation of CO2-responsive genes that may function as part of a carbon-concentrating mechanism (CCM) in photosynthetic eukaryotes. By comparing expression profiles of cells grown under CO2-rich conditions with those of cells grown under CO2-limiting conditions using a cDNA membrane array containing 10,368 expressed sequence tags, 51 low-CO2 inducible genes and 32 genes repressed by low CO2 whose mRNA levels were changed more than 2.5-fold in Chlamydomonas reinhardtii Dangeard were detected. The fact that the induction of almost all low-CO2 inducible genes was impaired in the ccm1 mutant suggests that CCM1 is a master regulator of CCM through putative low-CO2 signal transduction pathways. Among low-CO2 inducible genes, two novel genes, LciA and LciB, were identified, which may be involved in inorganic carbon transport. Possible functions of low-CO2 inducible and/or CCM1-regulated genes are discussed in relation to the CCM.
  • Isolation and characterization of Delta(6)-desaturase, an ELO-like enzyme and Delta(5)-desaturase from the liverwort Marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris, M Kajikawa, KT Yamato, Y Kohzu, M Nojiri, E Sakuradani, S Shimizu, Y Sakai, H Fukuzawa, K Ohyama, PLANT MOLECULAR BIOLOGY, PLANT MOLECULAR BIOLOGY, 54(3), 335 - 352, Feb. 2004 , Refereed
    Summary:The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and alpha-linolenic acids, respectively, by a series of reactions catalyzed by Delta(6)-desaturase, an ELO-like enzyme involved in Delta(6) elongation and Delta(5)-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6, MpELO1 and MpDES5, coding for the respective enzymes from M. polymorpha. Coexpression of the MpDES6, MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Interestingly, D6 desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other lower-plant Delta(6)-desaturases are known to have a strong preference for glycerolipids.
  • Expressed sequence tags from callus of Euphorbia tirucalli: A resource for genes involved in triterpenoid and sterol biosynthesis, Masataka Kajikawa, Katsuyuki T. Yamato, Yoshito Kohzu, Ryoko Sakata, Hideya Fukuzawa, Hidenobu Uchida, Kanji Ohyama, Plant Biotechnology, Plant Biotechnology, 21(5), 349 - 353, 2004 , Refereed
    Summary:We report generation of 9,301 expressed sequence tags (ESTs) derived from callus cells of Euphorbia tirucalli in search of candidate genes involved in the triterpenoid and sterol biosyntheses. After assembling 4,342 redundant ESTs into 1,252 clusters, a total of 6,211 non-redundant sequences were obtained. Database search revealed that 4,449 out of the 6,211 sequences shared significant similarities to known nucleotide or amino acid sequences, while the remaining 1,762 showed no significant matches and appear to represent novel genes in E. tirucalli. The annotations assigned to the hit database entries suggest that 48 of the unique sequences are involved in triterpenoid and sterol biosyntheses. Although functions of genes tagged by the 48 sequences are yet to be determined, the EST resource described here should contribute to identification of genes participating in the triterpenoid and sterol biosyntheses in E. tirucalli.
  • Plant regeneration from internode explants of Euphorbia tirucalli, Hidenobu Uchida, Osamu Nakayachi, Motoyasu Otani, Masataka Kajikawa, Yoshihito Kohzu, Katsuyuki T. Yamato, Hideya Fukuzawa, Takiko Shimada, Kanji Ohyama, Plant Biotechnology, Plant Biotechnology, 21(5), 397 - 399, 2004 , Refereed
    Summary:Euphorbia tirucalli is a potential source of commercially important chemicals such as sterols. Here we report the first successful plant regeneration from internode explants of E. tirucalli. Adventitious buds were efficiently induced on LS medium supplemented with 0.02 mg l-1 thidiazuron. On average of four experiments, 17.3 adventitious buds were induced from 12 explants on this medium. The adventitious buds grew into shoots during subsequent cultures on a hormone-free LS medium. For rooting treatment, we cultured these shoots on the LS medium containing 0.02 mg l-1 naphthalenacetic acid, followed by on the half-strength LS medium without vitamins, and were successful to obtain whole plantlets.
  • A mutant with constitutive sexual organ development in Marchantia polymorpha L., S Yamaoka, M Takenaka, T Hanajiri, Y Shimizu-Ueda, H Nishida, KT Yamato, H Fukuzawa, K Ohyama, SEXUAL PLANT REPRODUCTION, SEXUAL PLANT REPRODUCTION, 16(5), 253 - 257, Jan. 2004 , Refereed
    Summary:In lower land plants, genes controlling the transition from vegetative growth to sexual reproduction have not yet been identified. In the dioecious liverwort Marchantia polymorpha, the transition to sexual reproduction accompanied by the formation of sexual organs on the gametophytic thallus is initiated under long-day conditions. By particle bombardment-mediated mutagenesis, we generated a mutant of M. polymorpha that constitutively forms sexual organs. This mutant is fully fertile, showing that the mutation does not affect formation of male or female sexual organs per se. Genetic analysis reveals that this phenotype is caused by mutation of a single autosomal locus, suggesting that this mutation defines or controls a gene regulating the transition to sexual reproduction in M. polymorpha.
  • Functional analysis of a beta-ketoacyl-CoA synthase gene, MpFAE2, by gene silencing in the liverwort Marchantia polymorpha L., M Kajikawa, S Yamaoka, KT Yamato, H Kanamaru, E Sakuradani, S Shimizu, H Fukuzawa, K Ohyama, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 67(3), 605 - 612, Mar. 2003 , Refereed
    Summary:We have isolated a beta-ketoacyl CoA synthase (KCS) gene, MpFAE2, from a liverwort, Marchantia polymorpha, and identified its substrate specificity using the technique of dsRNA-mediated gene silencing and overexpression. KCS catalyzes an essential reaction in the fatty acid elongation process, i.e., condensation of malonyl-CoA with acyl-CoA. By introducing a construct with a hairpin structure containing a partial MpFAE2 gene, the level of the MpFAE2 gene expression was suppressed constitutively. The transgenic plants showed a specific accumulation of fatty acid 18:0. In contrast, in transgenic M. polymorpha plants overexpressing the MpFAE2 gene, fatty acid 22:0 is accumulated. These results indicate that the MpFAE2 gene product catalyzes the elongation steps of 18:0 to 20:0 and possibly also of 20:0 to 22:0.
  • Evolution of ribosomal DNA unit on the X chromosome independent of autosomal units in the liverwort Marchantia polymorpha, M Fujisawa, S Nakayama, T Nishio, M Fujishita, K Hayashi, K Ishizaki, M Kajikawa, KT Yamato, H Fukuzawa, K Ohyama, CHROMOSOME RESEARCH, CHROMOSOME RESEARCH, 11(7), 695 - 703, 2003 , Refereed
    Summary:In the haploid dioecious liverwort, Marchantia polymorpha, the X chromosome, but not the Y, carries a cluster of ribosomal RNA genes (rDNAs). Here we show that sequences of 5S, 17S, 5.8S and 26S rDNAs are highly conserved(> 99% identity) between the X chromosomal and autosomal rDNA repeat units, but the intergenic spacer sequences differ considerably. The most prominent difference is the presence of a 615-bp DNA fragment in the intergenic spacer, X615, which has accumulated predominantly in the rDNA cluster of the X chromosome. These observations suggest that the rDNA repeat unit on the X chromosome evolved independently of that on autosomes, incorporating sex chromosome-specific sequences.
  • Multicopy genes uniquely amplified in the Y chromosome-specific repeats of the liverwort Marchantia polymorpha, K Ishizaki, Y Shimizu-Ueda, S Okada, M Yamamoto, M Fujisawa, KT Yamato, H Fukuzawa, K Ohyama, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 30(21), 4675 - 4681, Nov. 2002 , Refereed
    Summary:Sex of the liverwort Marchantia polymorpha is determined by the sex chromosomes Y and X, in male and female plant, respectively. Approximately half of the Y chromosome is made up of unique repeat sequences. Here, we report that part of the Y chromosome, represented by a 90-kb insert of a genomic clone pMM2D3, contains five putative genes in addition to the ORF162 gene, which is present also within the Y chromosome-specific repeat region. One of the five putative genes shows similarity to a male gamete-specific protein of lily and is expressed predominantly in male sex organs, suggesting that this gene has a male reproductive function. Furthermore, Southern blot analysis revealed that these five putative genes are amplified on the Y chromosome, but they also probably have homologs on the X chromosome and/or autosomes. These observations suggest that the Y chromosome evolved by co-amplifying protein-coding genes with unique repeat sequences.
  • Isolation of X and Y chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis, M Fujisawa, K Hayashi, T Nishio, T Bando, S Okada, KT Yamato, H Fukuzawa, K Ohyama, GENETICS, GENETICS, 159(3), 981 - 985, Nov. 2001 , Refereed
    Summary:The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the Successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.
  • The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene, S Okada, T Sone, M Fujisawa, S Nakayama, M Takenaka, K Ishizaki, K Kono, Y Shimizu-Ueda, T Hanajiri, KT Yamato, H Fukuzawa, A Brennicke, K Ohyama, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 98(16), 9454 - 9459, Jul. 2001 , Refereed
    Summary:The haploid liverwort Marchantia polymorpha has heteromorphic sex chromosomes, an X chromosome in the female and a Y chromosome in the male. We here report on the repetitive structure of the liverwort Y chromosome through the analysis of male-specific P1-derived artificial chromosome (PAC) clones, pMM4G7 and pMM23-130F12. Several chromosome-specific sequence elements of approximate to 70 to 400 nt are combined into larger arrangements, which in turn are assembled into extensive Y chromosome-specific stretches. These repeat sequences contribute 2-3 Mb to the Y chromosome based on the observations of three different approaches: fluorescence in situ hybridization, dot blot hybridization, and the frequency of clones containing the repeat sequences in the genomic library. A novel Y chromosome-specific gene family was found embedded among these repeat sequences. This gene family encodes a putative protein with a RING finger motif and is expressed specifically in male sexual organs. To our knowledge, there have been no other reports for an active Y chromosome-specific gene in plants. The chromosome-specific repeat sequences possibly contribute to determining the identity of the Y chromosome in M. polymorpha as well as to maintaining genes required for male functions, as in mammals such as human.
  • Construction of male and female PAC genomic libraries suitable for identification of Y-chromosome-specific clones from the liverwort, Marchantia polymorpha, S Okada, M Fujisawa, T Sone, S Nakayama, R Nishiyama, M Takenaka, S Yamaoka, M Sakaida, K Kono, M Takahama, KT Yamato, H Fukuzawa, A Brennicke, K Ohyama, PLANT JOURNAL, PLANT JOURNAL, 24(3), 421 - 428, Nov. 2000
    Summary:Unlike higher plants, the dioecious liverwort, Marchantia polymorpha, has uniquely small sex chromosomes, with X chromosomes present only in female gametophytes and Y chromosomes only in male gametophytes. We have constructed respective genomic libraries for male and female plantlets using a P1-derived artificial chromosome (pCYPAC2). With an average insert size of approximately 90 kb, each PAC library is estimated to cover the entire genome with a probability of more than 99.9%. Male-specific PAC clones were screened for by differential hybridization using male and female genomic DNAs as separate probes. Seventy male-specific PAC clones were identified. The male specificity of one of the clones, pMM4G7, was verified by Southern hybridization and PCR analysis. This clone was indeed located on the Y chromosome as verified by fluorescence in situ hybridization (FISH). This result shows that the Y chromosome contains unique sequences that are not present either on the X chromosome or any of the autosomes. Thus, the respective male and female libraries for M. polymorpha offer an opportunity to identify key genes involved in the process of sex differentiation and this unique system of sex determination.
  • Comparison of expressed sequence tags from male and female sexual organs of Marchantia polymorpha, R Nishiyama, KT Yamato, K Miura, M Sakaida, S Okada, K Kono, M Takahama, T Sone, M Takenaka, H Fukuzawa, K Ohyama, DNA RESEARCH, DNA RESEARCH, 7(3), 165 - 174, Jun. 2000
    Summary:A total of 935 expressed sequence tags (ESTs) from male immature sexual organ were determined, of which 600 ESTs were assembled into 110 non-redundant groups, resulting in 445 unique EST sequences. Of these, 244 sequences shared significant similarities to known nucleotide or amino acid sequences in other organisms. The remaining 201 unique sequences showed no significant matches and thus are likely to be novel transcripts. ESTs from male and female immature sexual. organs of a liverwort, Marchantia polymorpha, were compared to characterize gene expression patterns during sex differentiation. Ninety-nine male ESTs turned out to be common genes found also in the female library. Interestingly, one of the ESTs found only in male shows a significant similarity to the transformer-2 gene involved in sex determination in Drosophila. In female, several unique lectin ESTs were found that are not present in the male library.
  • Direct transformation and plant regeneration of the haploid liverwort Marchantia polymorpha L., M Takenaka, S Yamaoka, T Hanajiri, Y Shimizu-Ueda, KT Yamato, H Fukuzawa, K Ohyama, TRANSGENIC RESEARCH, TRANSGENIC RESEARCH, 9(3), 179 - 185, Jun. 2000
    Summary:Thalli of the haploid liverwort Marchantia polymorpha were successfully used for direct particle bombardment with plasmid pMT, which carries a hygromycin phosphotransferase gene (hpt) controlled by the CaMV 35S promoter and the NOS polyadenylation region. Hygromycin-resistant cell masses arose from the thallus surface and developed directly into hygromycin-resistant thalli. Southern blot analyses indicated that these thalli carried at least 1-4 copies of the hpt gene, which were stably transmitted to their asexual thallus progenies via gemma propagation for three generations. This transformation and direct plant regeneration protocol is expected to be a valuable tool for the molecular analysis of this lower land plant.
  • Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants, T Sone, M Fujisawa, M Takenaka, S Nakagawa, S Yamaoka, M Sakaida, R Nishiyama, KT Yamato, N Ohmido, K Fukui, H Fukuzawa, K Ohyama, PLANT MOLECULAR BIOLOGY, PLANT MOLECULAR BIOLOGY, 41(5), 679 - 685, Nov. 1999 , Refereed
    Summary:The 5S ribosomal RNA genes (5S rDNA) are located independently from the 45S rDNA repeats containing 18S, 5.8S and 26S ribosomal RNA genes in higher eukaryotes. Southern blot and fluorescence in situ hybridization analyses demonstrated that the 5S rDNAs are encoded in the 45S rDNA repeat unit of a liverwort, Marchantia polymorpha, in contrast to higher plants. Sequencing analyses revealed that a single-repeat unit of the M. polymorpha nuclear rDNA, which is 16 103 bp in length, contained a 5S rDNA downstream of 18S, 5.8S and 26S rDNA. To our knowledge, this is the first report on co-localization of the 5S and 45S rDNAs in the rDNA repeat of land plants. Furthermore, we detected a 5S rDNA in the rDNA repeat of a moss, Funaria hygrometrica, by a homology search in a database. These findings suggest that there has been structural re-organization of the rDNAs after divergence of the bryophytes from the other plant species in the course of evolution.
  • Heteroplasmy and homoplasmy for maize mitochondrial mutants: A rare homoplasmic nad4 deletion mutant plant, KT Yamato, KJ Newton, JOURNAL OF HEREDITY, JOURNAL OF HEREDITY, 90(3), 369 - 373, May 1999
    Summary:The nonchromosomal stripe (NCS) mutants of maize are a set of mitochondrial deletion mutants. In aerobic organisms, deletions of essential mitochondrial genes are lethal. Therefore, most plants carrying deletions of part of essential mitochondrial genes survive only heteroplasmically; that is, the plants contain mixtures of the deleted and normal mitochondrial genomes. The severity of the mutant phenotype depends on which mitochondrial gene is altered, which also influences the extent of homoplasmic tissues on the plants. NCS2 plants carry a partial deletion of the nad4 gene that codes for a subunit of complex I. Unlike animals, plants have additional enzymes that can partially compensate for the loss of complex I function. Kernels that are homoplasmic for the nad4 deletion mutation usually abort, although we report that homoplasmic mutant cultures can be initiated and maintained from immature embryos of such kernels. We also report the first example of a rare homoplasmic mutant NCS2 plant. The plant is very short, pale green, with a reduced number of narrow leaves. It is apparently male and female sterile, The possibility that the kernel that gave rise to this plant had a heteroplasmic endosperm is discussed.
  • Expressed sequence tags from immature female sexual organ of a liverwort, Marchantia polymorpha, Jun-Ichi Nagai, Katsuyuki T. Yamato, Megumi Sakaida, Hiroshi Yoda, Hideya Fukuzawa, Kanji Ohyama, DNA Research, DNA Research, 6(1), 1 - 11, 1999
    Summary:A total of 970 expressed sequence tag (EST) clones were generated from immature female sexual organ of a liverwort, Marchantia polymorpha. The 376 ESTs resulted in 123 redundant groups, thus the total number of unique sequences in the EST set was 717. Database search by BLAST algorithm showed that 302 of the unique sequences shared significant similarities to known nucleotide or amino acid sequences. Six unique sequences showed significant similarities to genes that are involved in flower development and sexual reproduction, such as cynarase, fimbriata-associated protein and S-receptor kinase genes. The remaining unique 415 sequences have no significant similarity with any database-registered genes or proteins. The redundant 123 ESTs implied the presence of gene families and abundant transcripts of unknown identity. Analyses of the coding sequences of 61 unique sequences, which contained no ambiguous bases in the predicted coding regions, highly homologous to known sequences at the amino acid level with a similarity score greater than 400, and with stop codons at similar positions as their possible orthologues, indicated the presence of biased codon usage and higher GC content within the coding sequences (50.4%) than that within 3′ flanking sequences (41.9%).
  • EVIDENCE FOR A NOVEL MITOCHONDRIAL PROMOTER PRECEDING THE COX2 GENE OF PERENNIAL TEOSINTES, KJ NEWTON, B WINBERG, K YAMATO, S LUPOLD, DB STERN, EMBO JOURNAL, EMBO JOURNAL, 14(3), 585 - 593, Feb. 1995
    Summary:We have characterized two promoters of the cytochrome oxidase subunit 2 (cox2) gene in Zea perennis mitochondria present in maize lines. Initiation at a site 907 bases upstream of the start codon results in the major similar to 1900 nt cox2 transcript. A sequence just upstream of this site conforms to the consensus described for maize mitochondrial promoters and its transcription is correctly initiated in a maize mitochondrial in vitro transcription extract. A second transcription initiation site (-347) is used only when the dominant allele of a nuclear gene, Mct, is present and its use results in an additional, shorter major transcript, Sequences flanking the Mct-dependent transcription initiation site, which we have termed the conditional promoter of cox2 (cpc), do not fit the maize mitochondrial promoter consensus and do not function in the maize in vitro transcription extract. The cpc region does not hybridize with mitochondrial, chloroplast or nuclear DNAs from most maize or teosinte lines, However, the cpc sequence is found in the same position upstream of the cox2 gene in Zea diploperennis mtDNA and it has striking similarity to the previously reported 'ORF of unknown origin' fused to the ATPase subunit 6 gene in maize CMS-C mitochondria, cpc appears to represent a new type of mitochondrial promoter, Further analysis of both conditional and constitutive promoters should help us to better understand the control of transcription in plant mitochondria.
  • OCCURRENCE AND TRANSCRIPTION OF GENES FOR NAD1, NAD3, NAD4L, AND NAD6, CODING FOR NADH DEHYDROGENASE SUBUNIT-1, SUBUNIT-3, SUBUNIT-4L, AND SUBUNIT-6, IN LIVERWORT MITOCHONDRIA, K YAMATO, N NOZATO, K ODA, E OHTA, M TAKEMURA, K AKASHI, K OHYAMA, CURRENT GENETICS, CURRENT GENETICS, 23(5-6), 526 - 531, May 1993
    Summary:The genes encoding subunits 1, 3, 4L, and 6 of NADH dehydrogenase (nad1, nad3, nad4L, nad6) in the mitochondrial genome of a liverwort, Marchantia polymorpha, were characterized by comparing homologies of the amino-acid sequences of the subunits with those of other organisms. The nad3 and nad4L genes are split by single and double group 11 introns, respectively. The 5'-half portion of the nad6 gene was repeated at an identity of 89% to form a reading frame consisting of 100 amino-acid residues. The Northern hybridization analysis showed that all four genes are transcribed in the liverwort mitochondria.
  • COTRANSCRIPTIONAL EXPRESSION OF MITOCHONDRIAL GENES FOR SUBUNITS OF NADH DEHYDROGENASE, NAD5, NAD4, NAD2, IN MARCHANTIA-POLYMORPHA, N NOZATO, K ODA, K YAMATO, E OHTA, M TAKEMURA, K AKASHI, H FUKUZAWA, K OHYAMA, MOLECULAR & GENERAL GENETICS, MOLECULAR & GENERAL GENETICS, 237(3), 343 - 350, Mar. 1993
    Summary:Three genes for the subunits of the NADH dehydrogenase (nad5, nad4, and nad2) are tandemly clustered on the liverwort mitochondrial genome. Their gene products showed high levels of amino acid sequence identity with the corresponding subunits from higher plant mitochondria (82.8-84.4%), and significant levels of identity with those from liverwort chloroplast (32.0-33.5%), Podospora anserina mitochondria (21.4-45.9%), and human mitochondria (18.4-27.9%). In addition, these three subunits from liverwort mitochondria have conserved amino acid residues in their central regions. The gene nad5 is interrupted by a 672 bp group I intron, while genes nad4 and nad2 are interrupted by group II introns of 899 bp and 1418 bp, respectively. Northern blot analysis using exon-intron specific probes indicated that these three genes are transcribed as a single precursor mRNA of 9.6 kb in length and are processed into mature mRNA molecules in liverwort mitochondria. Several regions of this nad gene cluster are repeated in the liverwort mitochondrial genome.
  • GROUP-I INTRONS IN THE LIVERWORT MITOCHONDRIAL GENOME - THE GENE CODING FOR SUBUNIT-1 OF CYTOCHROME-OXIDASE SHARES 5 INTRON POSITIONS WITH ITS FUNGAL COUNTERPARTS, E OHTA, K ODA, K YAMATO, Y NAKAMURA, M TAKEMURA, N NOZATO, K AKASHI, K OHYAMA, F MICHEL, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 21(5), 1297 - 1305, Mar. 1993 , Refereed
    Summary:The complete nucleotide sequence of the mitochondrial DNA (mtDNA) from a liverwort, Marchantia polymorpha, contains thirty-two introns. Twenty-five of these introns possess the characteristic secondary structures and consensus sequences of group II introns. The remaining seven are group I introns, six of which happen to interrupt the gene coding for subunit 1 of cytochrome oxidase (cox1). Interestingly, the insertion sites of one group II and four group I introns in the cox1 gene coincide with those of the respective fungal mitochondrial introns. Moreover, comparison of the four group I introns with their fungal counterparts shows that group I introns inserted at identical genomic sites in different organisms are indeed related to one another, in terms of the peptide sequences generated from the complete or fragmental ORFs encoded by these introns. At the same time, the liverwort introns turned out to be more divergent from their fungal cognates than the latter are from one another. We therefore conclude that vertical transmission from a common ancestor organism is the simplest explanation for the presence of cognate introns in liverwort and fungal mitochondrial genomes.
  • TRANSFER-RNA GENES IN THE MITOCHONDRIAL GENOME FROM A LIVERWORT, MARCHANTIA-POLYMORPHA - THE ABSENCE OF CHLOROPLAST-LIKE TRANSFER-RNAS, K ODA, K YAMATO, E OHTA, Y NAKAMURA, M TAKEMURA, N NOZATO, K AKASHI, K OHYAMA, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 20(14), 3773 - 3777, Jul. 1992
    Summary:Twenty-nine genes for 27 species of tRNAs were deduced from the complete nucleotide sequence of the mitochondrial genome from a liverwort, Marchantia Polymorpha. One to three species of tRNA genes corresponded to each of 20 amino acids including three species for leucine and arginine, two species for serine and glycine, and one for the rest of the amino acids. Interestingly, all tRNA genes were located in the semicircle of the liverwort mitochondrial genome except for the trnY and trnR genes. The region containing these tRNA genes was originally duplicated, and two trnR genes have diverged from each other. On the other hand, trnY and trnfM are present as two identical copies. The G:U and U:N wobbling between the first nucleotide of the anticodon and the third nucleotide of the codon permit the 27 tRNA identified species to translate almost all codons. However, at least two additional tRNA genes, trnI-GAU for AUY codon and trnT-UGU for ACR codon, are required to read all codons used in the liverwort mitochondrial genome. All of the identified tRNA genes are 'native' in liverwort mitochondria, not 'chloroplast-like' tRNAs as are found in the mitochondria of higher plants. This result implies that the tRNA gene transfer from chloroplast to mitochondrial genome in higher plants has occurred after the divergence from bryophytes.
  • GENE CLUSTERS FOR RIBOSOMAL-PROTEINS IN THE MITOCHONDRIAL GENOME OF A LIVERWORT, MARCHANTIA-POLYMORPHA, M TAKEMURA, K ODA, K YAMATO, E OHTA, Y NAKAMURA, N NOZATO, K AKASHI, K OHYAMA, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 20(12), 3199 - 3205, Jun. 1992
    Summary:We detected 16 genes for ribosomal proteins in the complete sequence of the mitochondrial DNA from a liverwort, Marchantia polymorpha. The genes formed two major clusters, rps12-rps7 and rps10-rp12-rps19-rps3-rpl16-rp15-rps14-rps8-rp16-rps13-rps11-rps1, very similar in organization to Escherichia coli ribosomal protein operons (str and S10-spc-alpha operons, respectively). In contrast, rps2 and rps4 genes were located separately in the liverwort mitochondrial genome (the latter was part of the alpha-operon in E. coli). Furthermore, several ribosomal proteins encoded by the liverwort mitochondrial genome differed substantially in size from their counterparts in E. coli and liverwort chloroplast.
  • Complete Nucleotide Sequence of the Mitochondrial DNA from a Liverwort, Marchantia polymorpha, Kenji Oda, Katsuyuki Yamato, Eiji Ohta, Yasukazu Nakamura, Miho Takemura, Naoko Nozato, Kinya Akashi, Takeshi Kanegae, Yutaka Ogura, Takayuki Kohchi, Kanji Ohyama, PLANT MOLECULAR BIOLOGY REPORTER, PLANT MOLECULAR BIOLOGY REPORTER, 10(2), 105 - 163, May 1992
    Summary:Libraries of cosmid and plasmid clones covering the entire region of mtDNA from the liverwort Marchantia polymorpha were constructed. These clones were used for the determination of the complete nucleotide sequence of the liverwort mtDNA totally 186,608 bp (GenBank no. M68929) and including genes for 3 species of ribosomal RNAs, 29 genes for 27 species of transfer RNAs, and 30 genes for functionally known proteins ( 16 ribosomal proteins, 3 subunits of cytochrome coxidase, apocytochrome b protein, 3 subunits of H*-ATPase, and 7 subunits of NADH ubiquinone oxidoreductase). The genome also contains 32 unidentified open reading frames. Thus the complete nucleotide sequences from both chloroplast and mitochondrial genomes have been determined in the same organism. Plasmid clones are available upon the request.
  • CLONING AND NUCLEOTIDE-SEQUENCE OF A FRXC-ORF469 GENE-CLUSTER OF SYNECHOCYSTIS PCC6803 - CONSERVATION WITH LIVERWORT CHLOROPLAST FRXC-ORF465 AND NIF OPERON, Y OGURA, M TAKEMURA, K ODA, K YAMATO, E OHTA, H FUKUZAWA, K OHYAMA, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 56(5), 788 - 793, May 1992 , Refereed
    Summary:A gene, frxC, which is unique to the chloroplast genome of the liverwort Marchantia polymorpha, has sequence similarity to nifH, the product of which is an iron protein of a nitrogenase. Although frxC is expressed to produce a protein in liverwort chloroplasts, its function is not known. Using a probe of liverwort chloroplast DNA, a 10.1-kb region containing a gene cluster consisting of open reading frames (ORF278-frxC-ORF469-ORF248) was isolated from the cyanobacterium Synechocystis PCC6803. In this region, frxC and ORF469 showed sequence similarities to liverwort chloroplast frxC (83%) and immediately downstream ORF465 (74%), respectively. Synechocystis frxC showed 31% amino acid sequence identity with nifH1 from Clostridium pasteurianum. Additionally, Synechocystis ORF469 showed a sequence similarity (19% identity) to C. pasteurianum nifK product, which is the beta-subunit of a molybdenum-iron protein of a nitrogenase complex. Conservation of the gene arrangement between liverwort and Synechocystis suggests that the liverwort chloroplast frxC-ORF465 cluster may have evolved from an ancestor common to Synechocystis, and that these two genes may have been transferred to the nuclear genome in tobacco and rice during evolution.
  • MITOCHONDRIAL GENOME STRUCTURE OF RICE SUSPENSION-CULTURE FROM CYTOPLASMIC MALE-STERILE LINE (A-58CMS) - REAPPRAISAL OF THE MASTER CIRCLE, K YAMATO, Y OGURA, T KANEGAE, Y YAMADA, K OHYAMA, THEORETICAL AND APPLIED GENETICS, THEORETICAL AND APPLIED GENETICS, 83(3), 279 - 288, Jan. 1992 , Refereed
    Summary:The mitochondrial DNA (mtDNA) from the cultured cells of a cytoplasmic male-sterile line (A-58CMS) of rice (Oryza sativa) was cloned and its physical map was constructed. There was structural alteration on the mitochondrial genome during the cell culture. Detailed restriction analysis of cosmid clones having mtDNA fragments suggested either that the master genome has a 100-kb duplication (the genome size becomes 450 kb) or that a master circle is not present in the genome (the net structural complexity becomes 350 kb). The physical map of plant mitochondrial genomes thus far reported is illustrated in a single circle, namely a master circle. However, no circular DNA molecule corresponding to a master circle has yet been proved. In the present report, representation of plant mitochondrial genomes and a possibility for mitochondrial genome without a master circle are discussed.
  • GENE ORGANIZATION DEDUCED FROM THE COMPLETE SEQUENCE OF LIVERWORT MARCHANTIA-POLYMORPHA MITOCHONDRIAL-DNA - A PRIMITIVE FORM OF PLANT MITOCHONDRIAL GENOME, K ODA, K YAMATO, E OHTA, Y NAKAMURA, M TAKEMURA, N NOZATO, K AKASHI, T KANEGAE, Y OGURA, T KOHCHI, K OHYAMA, JOURNAL OF MOLECULAR BIOLOGY, JOURNAL OF MOLECULAR BIOLOGY, 223(1), 1 - 7, Jan. 1992 , Refereed
  • EVOLUTION OF ORGANELLAR GENOMES, K OHYAMA, Y OGURA, K ODA, K YAMATO, E OHTA, Y NAKAMURA, M TAKEMURA, N NOZATO, K AKASHI, T KANEGAE, Y YAMADA, EVOLUTION OF LIFE, EVOLUTION OF LIFE, 187 - 198, 1991 , Refereed
  • Gene organization and expression of chloroplast genome from a liverwort, Marchantia polymorpha., Ohyama, K, Kohchi, T, Ogura, Y, Oda, K, Yamato, K, Sano, T, Yamada, Y, Bot. Mag. Tokyo, Bot. Mag. Tokyo, 2, 145 - 158, 1990 , Refereed

Books etc

  • Evolution of organellar genomes, Ohyama, K, Ogura, Y, Oda, K, Yamato, K, Ohta, E, Nakamura, Y, Takemura, M, Nozato, N, Akashi, K, Kanegae, T, Yamada, Y, Joint author, Springer-Verlag,   1991 01

Conference Activities & Talks

  • Antheridium-specific voltage-gated ion channel is involved in sperm chemotaxis and male fertility in Marchantia polymorpha L., Taisuke Togawa, Noriyuki Suetsugu, Takayuki Kohchi, Katsuyuki T. Yamato, 12th International Symposium Exploring the Global Sustainability,   2019 08 06 , 招待有り
  • Identification of a putative sperm chemoattractant in the liverwort Marchantia polymorpha L., Yumiko Yamasaki, Miho Takemura, Tetsuya Matsukawa, Katsuyuki T. Yamato, Shin’ichiro Kajiyama, 12th International Symposium Exploring the Global Sustainability,   2019 08 06 , 招待有り
  • Molecular dissection of sperm chemotaxis in the liverwort Marchantia polymorpha L., Taisuke Togawa, Yumiko Yamasaki, Miho Takemura, Tetsuya Matsukawa, Noriyuki Suetsugu, Takayuki Kohchi, Shin’ichiro Kajiyama, Katsuyuki T. Yamato, 12th International Symposium Exploring the Global Sustainability,   2019 08 06 , 招待有り
  • Purification and structure estimation of a putative sperm chemoattractant in the liverwort Marchantia polymorpha, Yumiko YAMASAKI, Miho TAKEMURA, Tetsuya MATSUKAWA, Katsuyuki YAMATO, Shinichiro KAJIYAMA,   2019 03 27
  • Voltage-gated Ion Channel of SPERm1 is involved for chemotaxis and reproduction of spermatozoa in liverwort, Taisuke TOGAWA, Noriyuki SUETSUGU, Takayuki KOHCHI, Katsuyuki YAMATO,   2019 03 26
  • Germ cell differentiation requires the bHLH transcription factors BONOBOs evolutionarily conserved in land plants, Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, Keisuke Inoue, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, EMBO Workshop: New shores in land plant evolution,   2018 06 23
  • DNA-free genome editing by microinjection in Marchantia polymorpha, Shuichiro Aso, Takashi Takeuchi, Kiminari Yajima, Yuto Seki, Yoshihiro Takikawa, Shigeo S. Sugano, Aino Komatsu, Shohei Yamaoka, Ryuichi Nishihama, Takayuki Kohchi, Katsuyuki T.Yamato, EMBO Workshop: New shores in land plant evolution,   2018 06 21
  • Cryopreservation of Marchantia polymorpha spermatozoa, Taisuke Togawa, Tohru Adachi, Daijiro Harada, Tasuku Mitani, Daisuke Tanaka, Kimitsune Ishizaki, Takayuki Kohchi, Katsuyuki T. Yamato, EMBO Workshop: New shores in land plant evolution,   2018 06 21
  • Development of Marchantia Gene Nomenclature Database, Yasuhiro Tanizawa, Takako Mochizuki, Shohei Yamaoka, Ryuichi Nishihama, Takehiko Kanazawa, Takashi Ueda, Katsuyuki T. Yamato, Takayuki Kohchi, Yasukazu Nakamura, EMBO Workshop: New shores in land plant evolution,   2018 06 21
  • MarpolBase: Construction of the Marchantia polymorpha Genome Database, Takako Mochizuki, Yasuhiro Tanizawa, Shohei Yamaoka, Ryuichi Nishihama, Takehiko Kanazawa, Takashi Ueda, Katsuyuki T. Yamato, Takayuki Kohchi, Yasukazu Nakamura, EMBO Workshop: New shores in land plant evolution,   2018 06 21
  • CRISPR/Cas9を用いたJatropha curcas L.のゲノム編集の試み, 藤波友樹, 児玉岳, 十川太輔, 松川哲也, 大和勝幸, 梶山慎一郎, 日本農芸化学会大会講演要旨集(Web),   2018 03 05
  • ゼニゴケ・ゲノムにおける植物ホルモン生合成・シグナル伝達系遺伝子, 山岡尚平, 西浜竜一, 石崎公庸, 大和勝幸, 河内孝之, 日本農芸化学会大会講演要旨集(Web),   2018 03 05
  • みどりの香り組成を制御する酵素,ヘキセナール還元酵素の同定とその機能の解明, 田中俊之, 池田彩菜, 志岐和美, 国広(長井)直子, 大和勝幸, 道羅英夫, 大西利幸, 肥塚崇男, 松井健二, 日本農芸化学会大会講演要旨集(Web),   2018 03 05
  • 生物画像解析ソフトImageJによるゼニゴケ精子の運動解析, 十川太輔, 原田大士朗, 大和勝幸, 日本植物生理学会年会(Web),   2018
  • 転写因子BONOBOは陸上植物の生殖系列細胞の分化に必要である, 山岡尚平, 西浜竜一, 吉竹良洋, 石田咲子, 井上佳祐, 齊藤美咲, 岡橋啓太郎, BAO Haonan, 西田浩之, 山口勝司, 重信秀治, 石崎公庸, 大和勝幸, 河内孝之, 日本植物生理学会年会(Web),   2018
  • Marpolbase: Construction of the Marchantia polymorpha genome database, Takako Mochizuki, Yasuhiro Tanizawa, Hideki Nagasaki, Shohei Yamaoka, Ryuichi Nishihama, Takehiko Kanazawa, Takashi Ueda, Katsuyuki T. Yamato, Takayuki Kohchi, Yasukazu Nakamura, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • BONOBOs are evolutionarily conserved transcription factors required for germ cell fate determination in land plants, Shohei Yamaoka, Ryuichi Nishihama, Yoshihiro Yoshitake, Sakiko Ishida, Keisuke Inoue, Misaki Saito, Keitaro Okahashi, Haonan Bao, Hiroyuki Nishida, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • Quantitative computational image analysis of spermatozoa motility of Marchantia polymorpha L., Taisuke Togawa, Daijiro Harada, Daisuke Tanaka, Katsuyuki T. Yamato, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • Development of the genome-wide gRNA design program which extracts gRNAs with reduced off-targets in Marchantia polymorpha, Masato Tai, Kotaro Ishino, Katsuyuki T. Yamato, Ryuichi Nishihama, Takayuki Kohchi, Yoichiro Fukao, Shigeo S. Sugano, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • Development of two Gateway binary vector series for the assembly of three DNA fragments and promoter analysis in Marchantia polymorpha, Shoji Mano, Ryuichi Nishihama, Sakiko Ishida, Kazumi Hikino, Maki Kondo, Katsuyuki T. Yamato, Takayuki Kohchi, Tsuyoshi Nakagawa, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • The role of DNA methylation in Marchantia polymorpha, Yoko Ikeda, Ryuichi Nishihama, Shohei Yamaoka, Mario A, Arteaga-Vazquez, Daniel Grimanelli, Robert A. Martienssen, Katsuyuki T. Yamato, Takayuki Kohchi, Takashi Hirayama, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16
  • Cryopreservation of Marchantia polymorpha, YAMATO Katsuyuki T, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16 , 招待有り
  • Sex chromosomes - the unique and sexy in the Marchantia genome, YAMATO Katsuyuki T, The 65th NIBB Conference: Marchantia Workshop 2017,   2017 12 16 , 招待有り
  • 超低温保存がゼニゴケ精子運動能に与える影響の定量化, 十川太輔, 原田大士朗, 田中大介, 田中大介, 大和勝幸, 日本農芸化学会中四国支部講演会講演要旨集(Web),   2017 09 21
  • ゼニゴケ小胞体ストレスセンサーIRE1の機能解析, 竹田翔, 十川太輔, 西浜竜一, 三柴啓一郎, 大和勝幸, 河内孝之, 岩田雄二, 小泉望, 日本農芸化学会中四国支部講演会講演要旨集(Web),   2017 09 21
  • 生物画像解析ソフトImageJによるゼニゴケ精子の運動解析, 十川太輔, 原田大士朗, 大和勝幸, 日本植物学会大会研究発表記録,   2017 09 01
  • ゼニゴケのPARNはミトコンドリアmRNAポリA鎖長を制御する, 金澤まい, 池田陽子, 西浜竜一, 山岡尚平, 大和勝幸, 河内孝之, 平山隆志, 平山隆志, 日本RNA学会年会要旨集,   2017 07 19
  • Analyses of the membrane trafficking system during spermatogenesis in Marchantia polymorpha, Naoki Minamino, Takehiko Kanazawa, Takuya Norizuki, Ryuichi Nishihama, Katsuyuki T. Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Akihiko Nakano, Takashi Ueda,   2017 03 18
  • MpAHG2 and MpAGS1, regulators for poly(A) status of mitochondrial mRNA in Marchantia polymorpha, Mai Kanazawa, Yoko Ikeda, Ryuichi Nishihama, Shohei Yamaoka, Katsuyuki T. Yamato, Takayuki Kohchi, Takashi Hirayama,   2017 03 18
  • トランスクリプトームとプロテオーム解析によるミドリサンゴの乳液の防御機能の研究, 北島佐紀人, 三浦謙治, 青木航, 青木航, 大和勝幸, 平良東紀, 村上隆太, 油屋駿介, 日本農芸化学会関西支部講演会講演要旨集,   2016 09 15
  • 植物の雄性配偶子形成に関する転写因子の進化:苔類ゼニゴケを用いた研究によりわかってきたこと, 肥後あすか, 河島友和, 石崎公庸, 大和勝幸, 河内孝之, BERGER Frederic, 荒木崇, 日本植物学会大会研究発表記録,   2016 09 01
  • トランスクリプトーム解析による苔類ゼニゴケの造精器および精子の発生に機能する因子の探索, 肥後あすか, 大和勝幸, 遠藤求, 河内孝之, 荒木崇, 日本植物学会大会研究発表記録,   2016 09 01
  • ゼニゴケの栄養繁殖器官におけるトランスクリプトーム解析, 塚本成幸, 大和勝幸, 山口勝司, 重信秀治, 深城英弘, 三村徹郎, 河内孝之, 石崎公庸, 日本植物学会大会研究発表記録,   2016 09 01
  • ゼニゴケX染色体に存在するREPRESSOR OF SILENCING 1ホモログMpROS1Xの機能解析, 十川太輔, 原田大士朗, 池田陽子, 塚本成幸, 石崎公庸, 丹羽優喜, 荒木崇, 山口勝司, 重信秀治, 河内孝之, 大和勝幸, 日本植物学会大会研究発表記録,   2016 09 01
  • The transcription factor BONOBO plays a central role in transition from vegetative to reproductive growth in the liverwort Marchantia polymorpha, Yamaoka et, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Critical role of the R2R3-MYB gene GEMMA CUP-ASSOCIATED MYB1 for vegetative propagation in the liverwort Marchantia polymorpha L., Tsukamoto e, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • RNA processing in plastids and mitochondria with a limited number of pentatricopeptide repeat (PPR) proteins in Marchantia polymorpha, Takenaka et, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Membrane trafficking system plays important roles in sperm function of Marchantia polymorpha, Minamino et, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Cell type-specific reorientation of a trafficking pathway led to acquisition of new organelles during land plant evolution, Kanazawa et, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Analysis of the genes for plant spermiogenesis using a liverwort Marchantia polymorpha, Asuka Higo, Katsuyuki Yamato, Tomokazu Kawashima, Kimitsune Ishizaki, Takayuki Kohchi, Frederic Berger, Takashi Araki, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Genome and Genomics in Marchantia polymorpha, Takayuki Kohchi, Katsuyuki Yamato, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama, John Bowman, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • Characterization of MpROS1X, an X-chromosomal homolog of REPRESSOR OF SILENCING 1, in the liverwort Marchantia polymorpha, TOGAWA Taisuke, HARADA Daijiro, TSUKAMOTO Shigeyuki, ISHIZAKI Kimitsune, NIWA Masaki, ARAKI Takashi, YAMAGUCHI Katsushi, SHIGENOBU Shuji, KOHCHI Takayuki, YAMATO Katsuyuki T, 日本植物生理学会年会要旨集,   2016 03 11
  • Day length- and light quality-dependent expression of BONOBO, a master regulatory gene for growth-phase transition in the liverwort Marchantia polymorpha, YAMAOKA Shohei, INOUE Keisuke, TOMOGANE Hirokazu, NISHIHAMA Ryuichi, YAMAGUCHI Katsushi, SHIGENOBU Shuji, ISHIZAKI Kimitsune, YAMATO Katsuyuki T, KOHCHI Takayuki, 日本植物生理学会年会要旨集,   2016 03 11
  • Functional characterization of GCAM1, an R2R3-MYB essential for the development of gemma cup in the liverwort Marchantia polymorpha L., TSUKAMOTO Shigeyuki, SUGAYA Tomomi, YAMATO Katsuyuki T, NISHIHAMA Ryuichi, KUBO Hiroyoshi, FUKAKI Hidehiro, MIMURA Tetsuro, KOHCHI Takayuki, ISHIZAKI Kimitsune, 日本植物生理学会年会要旨集,   2016 03 11
  • Functional diversification of SYP1 members in Marchantia polymorpha, KANAZAWA Takehiko, ERA Atsuko, ERA Atsuko, MINAMINO Naoki, MORINAKA Hatsune, NORIZUKI Takuya, FUJIMOTO Masaru, UEMURA Tomohiro, NISHIHAMA Ryuichi, YAMATO Katsuyuki T, ISHIZAKI Kimitsune, NISHIYAMA Tomoaki, KOHCHI Takayuki, NAKANO Akihiko, NAKANO Akihiko, UEDA Takashi, UEDA Takashi, 日本植物生理学会年会要旨集,   2016 03 11
  • 陸上植物固有のR‐SNAREに存在する挿入配列の機能とその起源, 藤本優, 海老根一生, 石崎公庸, 大和勝幸, 深尾陽一朗, 植村知博, 丸山桃子, 井坂奈々子, 堤伸浩, 河内孝之, 中野明彦, 中野明彦, 上田貴志, 上田貴志, 日本分子生物学会年会プログラム・要旨集(Web),   2016
  • Membrane traffic in spermatogenesis of Marchantia polymorpha, Minamino Naoki, Kanazawa Takehiko, Era Atsuko, Nishihama Ryuichi, Yamato Katsuyuki, Ishizaki Kimitsune, Kohchi Takayuki, Nakano Akihiko, Ueda Takashi, the 79th Annual Meeting of the Botanical Society of Japan,   2015 09
  • Toward understanding the molecular mechanism of spermatogenesis in plants using a liverwort Marchantia polymorpha as a model, Higo Asuka, Ishizaki Kimitsune, Yamato Katsuyuki, Kohchi Takayuki, Araki Takashi, the 79th Annual Meeting of the Botanical Society of Japan,   2015 09
  • The Sexy Genome and Cool Cryopreservation of Marchantia polymorpha, YAMATO Katsuyuki, German-Japanese Joint Seminar 2015,   2015 09 , 招待有り
  • ゼニゴケ精子超低温保存法の開発, TOGAWA TAISUKE, ADACHI TOORU, TANAKA DAISUKE, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, YAMATO KATSUYUKI, 日本植物学会大会研究発表記録,   2015 09 01
  • ゼニゴケ初期胚発生を制御する因子の探索, NIWA YUKI, SAKAI YUKI, ISHIDA TAKASHI, NISHIHAMA RYUICHI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, SAWA SHIN'ICHIRO, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2015 09 01
  • ゼニゴケの杯状体形成を制御するGCAM1の機能解析, TSUKAMOTO SHIGEYUKI, SUGAYA TOMOMI, YAMATO KATSUYUKI, NISHIHAMA RYUICHI, KUBO HIROYOSHI, FUKAKI HIDEHIRO, MIMURA TETSURO, KOUCHI TAKAYUKI, ISHIZAKI KIMITSUNE, 日本植物学会大会研究発表記録,   2015 09 01
  • ゼニゴケのSNARE分子から観る膜融合装置の保存性と多様性, KANAZAWA TAKEHIKO, ERA ATSUKO, ERA ATSUKO, MINAMINO NAOKI, KANO YU, FUJIMOTO MASARU, UEMURA TOMOHIRO, NISHIHAMA RYUICHI, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, NISHIYAMA TOMOAKI, KOUCHI TAKAYUKI, NAKANO AKIHIKO, NAKANO AKIHIKO, UEDA TAKASHI, UEDA TAKASHI, 日本植物学会大会研究発表記録,   2015 09 01
  • 転写因子BONOBOは苔類ゼニゴケの有性生殖器官形成を制御する, YAMAOKA SHOHEI, TOMOGANE HIROKAZU, NISHIHAMA RYUICHI, YAMAGUCHI KATSUSHI, SHIGENOBU SHUJI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2015 03 09
  • R2R3‐MYB型転写因子GCAM1は苔類ゼニゴケの杯状体形成を制御する, TSUKAMOTO SHIGEYUKI, SUGAYA TOMOMI, YAMATO KATSUYUKI, NISHIHAMA RYUICHI, KUBO HIROYOSHI, SHICHIJO CHIZUKO, FUKAKI HIDEHIRO, MIMURA TETSURO, KOUCHI TAKAYUKI, ISHIZAKI KIMITSUNE, 日本植物生理学会年会要旨集,   2015 03 09
  • ゼニゴケゲノムアノテーションデータベースの構築, NAGASAKI HIDEKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKAMURA YASUKAZU, 日本植物生理学会年会要旨集,   2015 03 09
  • 苔類ゼニゴケの雄性配偶子形成過程を制御する分子機構の解析, HIGO ASUKA, NIWA YUKI, YAMATO KATSUYUKI, SAKAMOTO TOMOAKI, KURATA TETSUYA, SHIRAKAWA MAKOTO, SHIGENOBU SHUJI, ISHIZAKI KIMITSUNE, NISHIHAMA RYUICHI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2015 03 09
  • 基部陸上植物ゼニゴケにおけるRAB GTPaseの網羅的解析, MINAMINO NAOKI, KANAZAWA TAKEHIKO, ERA ATSUKO, NISHIHAMA RYUICHI, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, NISHIYAMA TOMOAKI, KOUCHI TAKAYUKI, NAKANO AKIHIKO, UEDA TAKASHI, 日本植物生理学会年会要旨集,   2015 03 09
  • ゼニゴケ精子凍結保存法の開発, SOGAWA TAISUKE, ADACHI TOORU, TANAKA DAISUKE, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, YAMATO KATSUYUKI, 日本植物生理学会年会要旨集,   2015 03 09
  • ゼニゴケアラキドン酸由来炭素数8揮発性化合物の代謝経路, KIHARA HIROTOMO, TANAKA MAYA, YAMATO KATSUYUKI, YAMADA SHOJI, KITA SAYAKA, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, AKAKABE YOSHIHIKO, KOEZUKA TAKAO, MATSUI KENJI, 日本農芸化学会中四国支部講演会講演要旨集(Web),   2015 01 24
  • GEMMA CUP-ASSOCIATED MYB1 is essential for the development of gemmacup in the liverwort Marchantia polymorpha L., S. Tsukamoto, T. Sugaya, K.T. Yamato, K. Yamaguchi, S. Shigenobu, H. Kubo, H. Fukaki, T. Mimura, T. Kohchi, K. Ishizaki, Marchantia Workshop 2014,   2014 12
  • Sperm cryopreservation in Marchantia polymorpha L., T. Togawa, T. Adachi, D. Tanaka, K. Ishizaki, T. Kohchi, K. T. Yamato, Marchantia Workshop 2014,   2014 12
  • PPR proteins in Marchantia polymorpha - key factors of RNA processing in plant organelles, M. Takenaka, K. T. Yamato, Y. Matsuda, M. Shirakawa, S. Yamaoka, K. Ishizaki, T. Kohchi, Marchantia Workshop 2014,   2014 12
  • Phenotypic analyses of MpLFY knockout plants, M. Niwa, Y. Sakai, A. Higo, M. Endo, A. Yamaguchi, K. Ishizaki, K. T. Yamato, R. Nishihama, T. Kohchi, T. Araki, Marchantia Workshop 2014,   2014 12
  • Transformation of Marchantia polymorpha using the bar gene as a selection selection marker, T. Nishimura, T. Nakagawa, K. T. Yamato, Marchantia Workshop 2014,   2014 12
  • Phototropin encoded by a single-copy gene mediates chloroplast photorelocation movements in Marchantia polymorpha L., A. Komatsu, M. Terai, K. Ishizaki, N. Suetsugu, H. Tsuboi, R. Nishihama, K. T. Yamato, M. Wada, T. Kohchi, Marchantia Workshop 2014,   2014 12
  • Toward understanding of the molecular mechanism regulating the formation of the male gametophyte, A. Higo, M. Niwa, T. Sakamoto, K. Ishizaki, K. Yamato, M. Shirakawa, M. Endo, T. Kurata, R. Nishihama, T. Kohch, T. Araki, Marchantia Workshop 2014,   2014 12
  • The Marchantia genome: the final progress report., YAMATO Katsuyuki, Marchantia Workshop 2014,   2014 12 , 招待有り
  • Cryopreservation of Marchantia polymorpha., YAMATO Katsuyuki, Marchantia Workshop 2014,   2014 12 , 招待有り
  • C3/C4光合成相互転換植物Eleocharis viviparaを用いたC4光合成成立遺伝子の探索, HARADA DAIJIRO, SAKAMOTO TOMOAKI, KURATA TETSUYA, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物学会大会研究発表記録,   2014 09 01
  • ゼニゴゲの接合子におけるMpLFYの機能解析, NIWA YUKI, SAKAI YUKI, HIGO ASUKA, ENDO MOTOMU, YAMAGUCHI REIKO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, NISHIHAMA RYUICHI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2014 09 01
  • ゼニゴケの栄養繁殖器官で発現するR2R3型MYB遺伝子の単離と機能解析, TSUKAMOTO SHIGEYUKI, SUGAYA TOMOMI, YAMATO KATSUYUKI, YAMAGUCHI KATSUSHI, SHIGENOBU SHUJI, NISHIHAMA RYUICHI, SHICHIJO CHIZUKO, KUBO HIROYOSHI, KOUCHI TAKAYUKI, FUKAKI HIDEHIRO, MIMURA TETSURO, ISHIZAKI KIMITSUNE, 日本植物学会大会研究発表記録,   2014 09 01
  • ゼニゴケ無性芽の-80°Cフリーザーでの長期保存法の開発, TANAKA DAISUKE, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, 日本植物学会大会研究発表記録,   2014 09 01
  • 苔類ゼニゴケの造精器および精子の発生過程に関与する遺伝子発現プログラムを制御する機構の解析, HIGO ASUKA, NIWA YUKI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, NISHIHAMA RYUICHI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2014 09 01
  • ゼニゴケ炭素数8揮発性化合物の生合成経路の解明, KIHARA HIROTOMO, TANAKA MAYA, YAMATO KATSUYUKI, YAMADA SHOJI, KITA SAYAKA, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, AKAKABE YOSHIHIKO, KOEZUKA TAKAO, MATSUI KENJI, 日本農芸化学会中四国支部講演会講演要旨集,   2014 05 31
  • ゼニゴケ研究地平への投射:オーキシン信号伝達を例に, KOUCHI TAKAYUKI, KATO HIROKI, NISHIHAMA RYUICHI, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, 日本植物生理学会年会要旨集,   2014 03 11
  • 新興モデル生物ゼニゴケ無性芽の超低温保存法の開発, TANAKA DAISUKE, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2014 03 11
  • ゼニゴケMpLFYノックアウト株の表現型解析, NIWA YUKI, SAKAI YUKI, HIGO ASUKA, ENDO MOTOMU, YAMAGUCHI REIKO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, NISHIHAMA RYUICHI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2014 03 11
  • 次世代シーケンサーによるC3/C4光合成型Eleocharis viviparaのDe novoトランスクリプトーム解析, HARADA DAISHIRO, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2014 03 11
  • ゼニゴケゲノムアノテーションデータベースの構築, NAGASAKI HIDEKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKAMURA YASUKAZU, 日本分子生物学会年会プログラム・要旨集(Web),   2014
  • ゼニゴケ無性芽のガラス化法による超低温保存, TANAKA DAISUKE, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, 低温生物工学会セミナー及び年会講演要旨集,   2014
  • Sex in Marchantia polymorpha., YAMATO Katsuyuki, Marchantia Workshop 2013,   2013 12 , 招待有り
  • 基部陸上植物ゼニゴケにおける概日時計を介した成長相制御機構, KUBOTA AKANE, KITA SHOGO, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI YOSHIYUKI, OYAMA TOKITAKA, NISHIHAMA RYUICHI, KOUCHI TAKAYUKI, 時間生物学,   2013 10 30
  • ゼニゴケ炭素数8揮発性化合物の生合成経路の解明, KIHARA HIROTOMO, TANAKA MAYA, YAMATO KATSUYUKI, YAMADA ATSUSHI, KITA SAYAKA, ISHIZAKI KIMITSUNE, KOUCHI TAKAYUKI, AKAKABE YOSHIHIKO, MATSUI KENJI, 香料・テルペンおよび精油化学に関する討論会講演要旨集,   2013 10 05
  • C3,C4光合成型相互転換植物Eleocharis viviparaの成長点におけるトランスクリプトーム解析, HARADA DAISHIRO, SAKAMOTO TOMOAKI, KURATA TETSUYA, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2013 08 20
  • 陸上植物固有のR‐SNAREに存在する挿入配列の起源とその機能, FUJIMOTO MASARU, EBINE KAZUO, MARUYAMA MOMOKO, ISAKA NANAKO, UEMURA TOMOHIRO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKANO AKIHIKO, UEDA TAKASHI, 日本植物生理学会年会要旨集,   2013 03 14
  • 次世代シーケンサーによるC3/C4光合成型Eleocharis viviparaトランスクリプトームの比較, HARADA DAISHIRO, HIRATA ITSUKI, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2013 03 14
  • 基部陸上植物ゼニゴケにおける概日時計の分子ネットワーク, KUBOTA AKANE, KITA SHOGO, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI YOSHIYUKI, OYAMA TOKITAKA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2013 03 14
  • 原核生物型PEBPファミリータンパク質CORのシロイヌナズナとゼニゴケにおける機能解析, MORIHANA SAYURI, TAKEMOTO SATORU, TSUJII YUKA, ARITE TOMOTSUGU, TAKEMURA MIHO, SAKAI YUKI, YAMAGUCHI REIKO, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2013 03 14
  • ゼニゴケゲノム解析とゲノムアノテーションデータベースの構築, NAGASAKI HIDEKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKAMURA YASUKAZU, 日本分子生物学会年会プログラム・要旨集(Web),   2013
  • Function of ANGUSTIFOLIA, a plant homolog of CtBP/BARS, in bryophytes, N. Minamisawa, Y. Hashida, K. Takechi, M. Kajikawa, K.T. Yamato, T. Ueda, H. Tsukaya, H. Takano, Marchantia Workshop 2012,   2012 11
  • Molecular analysis of circadian clock and growth-phase transition in M. polymorpha, A. Kubota, S. Kita, T. Muranaka, K. Ishizaki, K. T. Yamato, T. Oyama, S. Aoki, T. Kohchi, Marchantia Workshop 2012,   2012 11
  • Functional analysis of a Y chromosomal gene with a cytochrome b5 domain in Marchantia polymorpha L., T. Hasegawa, K. Ishizaki, T. Kohchi, K. T. Yamato, Marchantia Workshop 2012,   2012 11
  • Transcriptomic and proteomic analyses on spermatozoa of Marchantia polymorpha., S. Tsukamoto, S. Hirao, L. Yamada, H. Sawada, K. Ishizaki, T. Kohchi, K.T. Yamato, Marchantia Workshop 2012,   2012 11
  • Sexual reproduction in Marchantia polymorpha - sex chromosomes and sperm chemotaxis., YAMATO Katsuyuki, Marchantia Workshop 2012,   2012 11
  • Sperm Chemotaxis in the Liverwort Marchantia polymorpha L., YAMATO Katsuyuki, International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. Joint Meeting of the 2nd Allo-Authentication Meeting and the 5th Egg-Coat Meeting,   2012 11 , 招待有り
  • 和歌山県天然記念物キイシモツケの分子系統分類学的および生理形態的性格付け:イワシモツケおよびトサシモツケとの比較, AKEDO ERIKA, HIRATA TOMOKO, KAMII KAZUYUKI, TAKAGI YUKO, MIZUNO TAKAFUMI, KOBAYASHI MAKOTO, KOIKE TAKAYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本植物学会大会研究発表記録,   2012 09 14
  • 次世代シーケンサーによるC3‐C4光合成相互転換植物Eleocharis viviparaのトランスクリプトーム解析, HARADA DAISHIRO, HIRATA ITSUKI, SATO AKANE, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2012 08 03
  • C3‐C4光合成相互転換植物Eleocharis viviparaのin vitroにおける光合成型の切換え, HARADA DAISHIRO, MIZOBATA NATSUKI, YOKOYAMA KANAKO, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物生理学会年会要旨集,   2012 03 09
  • 遺伝子機能を自在に研究できるモデルへ~ゲノム・突然変異体・形質転換~, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2012 03 09
  • 苔類ゼニゴケを用いた基部陸上植物の概日時計因子の解析, KUBOTA AKANE, KITA SHOGO, KUBOTA SAAYA, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI YOSHIYUKI, OYAMA TOKITAKA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2012 03 09
  • 花芽形成のマスター制御因子LEAFYの祖先的機能の探索, SAKAI YUKI, ITO MISA, KAWAMOTO ASAMI, MIYASHITA YUI, UYAMA KAZUKI, TSUJII YUKA, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2012 03 09
  • シロイヌナズナのホルムアルデヒドストレス応答と活性酸素種(ROS)の関与に関する解析, KUBO SHIGERU, SAKAKIBARA HITOSHI, KURUSU TAKAMITSU, KUCHITSU KAZUYUKI, YURIMOTO HIROYA, SAKAI YASUYOSHI, AKITA MOTOMU, YAMATO KATSUYUKI, IZUI KATSURA, 日本植物生理学会年会要旨集,   2012 03 09
  • DDBJパイプラインによるゼニゴケゲノム解析とゲノムアノテーションデータベースの構築, NAGASAKI HIDEKI, FUJISAWA TAKATOMO, MOCHIZUKI TAKAKO, SARUHASHI SATOSHI, KAMINUMA ERI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKAMURA YASUKAZU, 日本分子生物学会年会プログラム・要旨集(Web),   2012
  • 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの直接制御標的の探索, KAWAMOTO ASAMI, SAKAI YUKI, UYAMA KAZUKI, TSUJII YUKA, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2011 09 10
  • 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析, SAKAI YUKI, MIYASHITA YUI, UYAMA KAZUKI, TSUJII YUKA, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2011 09 10
  • “基部陸上植物”の光応答戦略―フィトクロムを介した光形態形成の分子機構―, ISHIZAKI KIMITSUNE, INOUE KEISUKE, HOSAKA MASASHI, KATAOKA HIDEO, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物学会大会研究発表記録,   2011 09 10
  • C3‐C4光合成の相互転換植物Eleocharis viviparaの先端分裂組織からの培養系の誘導とin vitroにおけるC3型とC4型の切り換え, HARADA DAISHIRO, MIZOBATA NATSUKI, YOKOYAMA KANAKO, YOSHIMURA KAZUE, YAMATO KATSUYUKI, IZUI KATSURA, AKITA MOTOMU, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • イネへのホルムアルデヒドの同化代謝系(リブロースモノリン酸経路)の導入, SUZUKI SHIORI, AKEDO ERISHU, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • ヒメツリガネゴケ(Physcomitrella patens)に見出された病害抵抗性遺伝子(R遺伝子)に関する研究, TANIGAKI YUSUKE, ITO KENJI, KOSAKA AKIKO, LEHTONEN MIKKO, THELANDER MATTIAS, YAMATO KATSUYUKI, AKITA MOTOMU, VALKONEN JARI P. T, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集,   2011 09 06
  • The Genome of Marchantia polymorpha., YAMATO Katsuyuki, XVIII International Botanical Congress, Melbourne, Australia,   2011 07 , 招待有り
  • 苔類ゼニゴケにおけるアブシジン酸応答性遺伝子発現機構の解析, KANEKO MIDORI, KOMATSU KENJI, AKTER KHALEDA, SAKATA YOICHI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, TAKEZAWA DAISUKE, 日本植物生理学会年会要旨集,   2011 03 11
  • γ線照射胞子より単離したゼニゴケオーキシン耐性株の解析, NONOMURA MAIKO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • ゼニゴケVAMP72スプライシングバリアントの機能解析, FUJIMOTO MASARU, MARUYAMA MOMOKO, EBINE KAZUO, ISAKA NANAKO, UEMURA TOMOHIRO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKANO AKIHIKO, UEDA TAKASHI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケにおけるMpIAAを介したオーキシン信号伝達の機能解析, KATO HIROKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケにおける計時機構と生長相制御の解析, KUBOTA AKANE, KUBOTA SAAYA, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI YOSHIYUKI, OYAMA TOKITAKA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケにおける赤色光受容体フィトクロムを介した生長制御, INOUE KEISUKE, ISHIZAKI KIMITSUNE, HOSAKA MASASHI, KATAOKA HIDEO, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備:T‐DNAタギング法による順遺伝学的解析手法の確立, MASUDA AKIHIDE, ISHIZAKI KIMITSUNE, SAIDA YUKA, MIZUTANI MIYA, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケの青色光受容体フォトトロピンの単離と青色光応答の解析, KOMATSU AINO, TSUBOI HIDENORI, SUETSUGU NORIYUKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, WADA MASAMITSU, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2011 03 11
  • 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析, SAKAI YUKI, MIYASHITA YUI, KAWAMOTO ASAMI, UYAMA KAZUKI, TSUJII YUKA, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2011 03 11
  • 半数体植物ゼニゴケにおける順遺伝学的解析手法の確立, ISHIZAKI KIMITSUNE, MASUDA AKIHIDE, SAIDA YUKA, MIZUTANI MIYA, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2011 03 05
  • リブロースモノリン酸経路(RuMP)の2種類の酵素の融合体をコードする合成遺伝子を導入したイネの作成と解析, SUZUKI SHIORI, AKEDO ERISHU, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 苔類ゼニゴケの青色光応答と青色光受容体フォトトロピンの解析, KOMATSU AINO, TSUBOI HIDENORI, SUETSUGU NORIYUKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, WADA MASAMITSU, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 苔類ゼニゴケにおける計時機構と生長相制御の解析, KUBOTA AKANE, KUBOTA SAYA, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI YOSHIYUKI, OYAMA TOKITAKA, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 苔類ゼニゴケの光受容体フィトクロムを介した細胞分裂制御, INOUE KEISUKE, ISHIZAKI KIMITSUNE, HOSAKA MASASHI, KATAOKA HIDEO, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2011 03 05
  • C3/C4光合成の相互転換植物,カヤツリグサ科のEleocharis viviparaのcDNAライブラリーの作成とC4型PEPCのゲノムDNA塩基配列の決定, HARADA DAIJIRO, KONDO CHIE, YONEHARA RYO, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 生化学,   2011
  • イネとタバコへのホルムアルデヒド固定能の付与:C1微生物のリブロースモノリン酸経路(RuMP)の2つの酵素の葉緑体における高発現にむけて, AKEDO ERIKA, SUZUKI SHIORI, FUKUI TAKATO, NAKAGAWA TSUYOSHI, SAKAKIBARA HITOSHI, YURIMOTO HIROYA, SAKAI YASUYOSHI, YAMATO KATSUYUKI, AKITA MOTOMU, IZUI KATSURA, 生化学,   2011
  • T‐DNAタギング法により単離された苔類ゼニゴケ気室形成異常株の解析, MIZUTANI MIYA, MASUDA AKIHIDE, SAIDA YUKA, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2011
  • 基部陸上植物ゼニゴケにおける概日時計関連因子の比較ゲノム解析, KUBOTA AKANE, KITA SHOGO, KUBOTA SAYA, MURANAKA TOMOAKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, AOKI SETSUYUKI, OYAMA TOKITAKA, KOUCHI TAKAYUKI, 生化学,   2011
  • 苔類ゼニゴケにおける赤色光依存的な細胞分裂制御, INOUE KEISUKE, ISHIZAKI KIMITSUNE, HOSAKA MASASHI, KATAOKA HIDEO, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2011
  • 苔類ゼニゴケにおける青色光依存的な葉緑体光定位運動と青色光受容体フォトトロピン, KOMATSU AINO, ASHIHARA YUKIKO, TSUBOI HIDENORI, SUETSUGU NORIYUKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, WADA MASAMITSU, KOUCHI TAKAYUKI, 生化学,   2011
  • γ線照射胞子から単離した苔類ゼニゴケオーキシン低感受性変異株の解析, ISHIZAKI KIMITSUNE, NONOMURA MAIKO, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2011
  • 苔類ゼニゴケにおけるアブシジン酸応答性遺伝子発現の解析, KANEKO MIDORI, KOMATSU KENJI, SAKATA YOICHI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, TAKEZAWA DAISUKE, 日本植物学会大会研究発表記録,   2010 09 08
  • 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析, SAKAI YUKI, MIYASHITA YUI, UYAMA KAZUKI, TSUJII YUKA, DAIMON YASUFUMI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2010 09 08
  • 陸上植物に固有の転写因子LFY, ARAKI TAKASHI, SAKAI YUKI, TSUJII YUKA, UYAMA KAZUKI, MIYASHITA YUI, KAWAMOTO ASAMI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物学会大会研究発表記録,   2010 09 08
  • モデル実験生物ゼニゴケのゲノム情報およびリソースの現状, YAMATO KATSUYUKI, 日本植物学会大会研究発表記録,   2010 09 08
  • 苔類ゼニゴケにおけるPEBP family遺伝子の機能解析, TAKEMOTO SATORU, TSUJII YUKA, ARITE TOMOTSUGU, TAKEMURA MIHO, SAKAI YUKI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2010 09 08
  • ゼニゴケ―アロ認証研究におけるモデル生物としての可能性, YAMATO KATSUYUKI, 日本動物学会大会予稿集,   2010 08 20
  • ゼニゴケFLO/LFY相同遺伝子の機能解析, UYAMA KAZUKI, MIYASHITA YUI, TSUJII YUKA, DAIMON YASUFUMI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2010 03 12
  • 苔類ゼニゴケを用いたフィトクロムを介する細胞応答の調節機構の解析, HOSAKA MASASHI, ISHIZAKI KIMITSUNE, INOUE KEISUKE, KATAOKA HIDEO, YAMATO KATSUYUKI, MATSUNAGA SACHIHIRO, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2010 03 12
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備V:核ゲノム情報解析とT‐DNAタグライン, MASUDA AKIHIDE, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2010 03 12
  • 基部陸上植物ゼニゴケにおけるオーキシン生理応答の観察と可視化, NONOMURA MAIKO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2010 03 12
  • 種子植物特異的なR‐SNAREの進化細胞生物学的解析, FUJIMOTO MASARU, EBINE KAZUO, ISAKA NANAKO, UEMURA TOMOHIRO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, NAKANO AKIHIKO, UEDA TAKASHI, 日本植物生理学会年会要旨集,   2010 03 12
  • 新規ゼニゴケ核ゲノム形質転換選抜用マーカーの開発, UEDA MINORU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, SHIKAUCHI TOSHIHARU, NISHIMURA YOSHIKI, 日本植物生理学会年会要旨集,   2010 03 12
  • 緑藻クラミドモナスのストレス応答をゲノムで見る:次世代シーケンサーを用いたゲノム発現データベースの構築, FUKUZAWA HIDEYA, KUBO KATSUAKI, YAMATO KATSUYUKI, SUZUKI YUTAKA, SUGANO SUMIO, ITO TAKEHIKO, TANIGUCHI TAKEAKI, KUROKI YOKO, TOYODA ATSUSHI, KOHARA YUJI, FUJIYAMA ASAO, 日本植物生理学会年会要旨集,   2010 03 12
  • 苔類ゼニゴケにおける光屈性と青色光受容体フォトトロピン遺伝子の解析, KOMATSU AINO, KUBOTA AKANE, SUETSUGU NORIYUKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, WADA MASAMITSU, KOUCHI TAKAYUKI, 生化学,   2009 09 25
  • 苔類ゼニゴケのフィトクロムを介する光応答, HOSAKA MASASHI, KATAOKA HIDEO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2009 09 25
  • 苔類ゼニゴケにおけるオーキシン信号伝達因子の同定, KATO HIROTAKA, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2009 09 25
  • 苔類ゼニゴケにおけるオーキシン応答の観察と可視化, NONOMURA MAIKO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 生化学,   2009 09 25
  • ゼニゴケFLO/LFY相同遺伝子の機能解析, UYAMA KAZUKI, TSUJII YUKA, MIYASHITA YUI, DAIMON YASUFUMI, ENDO MOTOMU, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2009 09 17
  • 苔類ゼニゴケにおけるABI1様プロテインホスファターゼの機能解析, TOGANE MASARU, KOMATSU KENJI, BHYAN SALMA BEGUM, SAKATA YOICHI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, TAKEZAWA DAISUKE, 日本植物学会大会研究発表記録,   2009 09 17
  • コケ植物におけるオルガネラ動態の解析, ERA ATSUKO, EBINE KAZUO, TOMINAGA MOTOKI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, HIGAKI TAKUMI, KUTSUNA NATSUMARO, HASEZAWA SEIICHIRO, NAKANO AKIHIKO, KOUCHI TAKAYUKI, UEDA TAKASHI, 日本植物学会大会研究発表記録,   2009 09 17
  • 微細藻類の遺伝子資源をゲノムから探る:緑藻クラミドモナスのゲノムデータベースの構築と有用遺伝子の探索, FUKUZAWA HIDEYA, KUBO TAKEAKI, YAMATO KATSUYUKI, FUJIYAMA ASAO, TOYODA ATSUSHI, KUROKI YOKO, ITO TAKEHIKO, TANIGUCHI TAKEAKI, SUZUKI YUTAKA, SUGANO SUMIO, KOHARA YUJI, マリンバイオテクノロジー学会大会講演要旨集,   2009 05 30
  • ゼニゴケにおけるアクチン繊維のユニークな動き, ERA ATSUKO, EBINE KAZUO, ISHIZAKI KIMITSUNE, TOMINAGA MOTOKI, SAITO CHIEKO, YAMATO KATSUYUKI, NAKANO AKIHIKO, KOUCHI TAKAYUKI, UEDA TAKASHI, 日本植物生理学会年会要旨集,   2009 03 16
  • ゼニゴケFLO/LFY相同遺伝子の単離と解析, TSUJII YUKA, UYAMA KAZUKI, OKADO SEIJI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, ARAKI TAKASHI, 日本植物生理学会年会要旨集,   2009 03 16
  • 緑藻クラミドモナスの完全長cDNA配列解析とゲノム構造解析, KUBO KAZUAKI, YAMATO KATSUYUKI, YAMANO TAKASHI, SUZUKI YUTAKA, SUGANO SUMIO, FUJIYAMA ASAO, KOHARA YUJI, ITO TAKEHIKO, FUKUZAWA HIDEYA, 日本植物生理学会年会要旨集,   2009 03 16
  • 苔類ゼニゴケのフィトクロムを介する光応答, HOSAKA MASASHI, KATAOKA HIDEO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2009 03 16
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備 IV〈GatewayバイナリーベクターとT‐DNAタグライン〉, ISHIZAKI KIMITSUNE, YUKAWA YOSHIYASU, MASUDA AKIHIDE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2009 03 16
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備 III〈EST情報の蓄積と遺伝地図の作製〉, TOMOGANE HIROKAZU, YAMATO KATSUYUKI, CHIYODA MASAHIRO, ISHIZAKI KIMITSUNE, SUZUKI YUTAKA, SUGANO SUMIO, SHIN'I TADASU, KOHARA YUJI, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2009 03 16
  • 苔類ゼニゴケにおける青色光応答の解析, KUBOTA AKANE, KOMATSU AINO, KATAOKA HIDEO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2009 03 16
  • プロテインホスファターゼ2Cによる苔類アブシジン酸応答の制御, TOGANE KEN, KOMATSU KENJI, SAKATA YOICHI, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, TAKEZAWA DAISUKE, 日本植物生理学会年会要旨集,   2009 03 16
  • プラスチドDNAはrRNAオペロンの下流領域に複製フォーク障壁をもつ, CHIYODA SHOTA, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2009 03 16
  • ゼニゴケFLO/LFY相同遺伝子の単離と解析, TSUJII YUKA, DAIMON YASUSHI, ISHIZAKI KIMIYASU, YAMATO KATSUYUKI, KAWACHI TAKAYUKI, ARAKI TAKASHI, 日本植物学会大会研究発表記録,   2008 09 25
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備 I〈交配法の確立と遺伝地図の作製〉, TOMOGANE HIROKAZU, YAMATO KATSUYUKI, CHIYODA MASAHIRO, KATAOKA HIDEO, ISHIZAKI KIMITSUNE, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2008 03 15
  • 苔類ゼニゴケをモデルとしたフィトクロムシグナル伝達の解析, KATAOKA HIDEO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2008 03 15
  • 苔類ゼニゴケにおけるプラスチドDNAの複製と遺伝様式の解析, CHIYODA MASAHIRO, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2008 03 15
  • 苔類ゼニゴケにおける分子遺伝学の基盤整備 II〈簡便かつ高効率なアグロバクテリウムによる形質転換〉, ISHIZAKI KIMITSUNE, CHIYODA MASAHIRO, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2008 03 15
  • 可変領域および動物型DNAメチルトランスフェラーゼドメインをもつゼニゴケLTR‐レトロトランスポゾン様転移因子DREの構造解析, YAMATO KATSUYUKI, YAMAKI ARATA, TSUCHIMOTO SUGURU, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2008 03 05
  • 苔類ゼニゴケクリプトクロム遺伝子の単離と構造解析, KUBOTA AKANE, KATAOKA HIDEO, ISHIZAKI KIMIYASU, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2008 03 05
  • ゼニゴケ染色体の全構造に基づく性染色体の進化様式, OYAMA KANJI, YAMATO KATSUYUKI, ISHIZAKI KIMITSUNE, FUJISAWA MASAKI, OKADA SHOKO, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, BANDO HIROKI, YODOYA KOHEI, HAYASHI KIWAKO, BANDO MICHIYUKI, HASUMI AKIKO, NISHIO TOMOHISA, SAKATA RYOKO, YAMAMOTO MASAYUKI, YAMAKI SHIN, NISHIIDE TAKU, CHOI SEUNG-HYUK, ARAI OSAMU, OHARA YUJI, KAWAUCHI TAKAYUKI, FUKUZAWA HIDEYA, 日本植物学会大会研究発表記録,   2007 09 06
  • 緑藻クラミドモナスのゲノムから見た光合成と生殖機能, FUKUZAWA HIDEYA, YAMANO TAKASHI, SATAKE TOMOYA, FUJITA AKIMITSU, ONISHI NORIKAZU, KOHINATA TSUTOMU, YAMAHARA YOSUKE, TSUJIKAWA TOMOKI, YAMATO KATSUYUKI, 日本植物学会大会研究発表記録,   2007 09 06
  • ゼニゴケゲノムにはDNAメチルトランスフェラーゼドメインをもつ新規転移因子が存在する, DAIWA KATSUYUKI, YAMAKI ARATA, TSUCHIMOTO SUGURU, FUKUZAWA HIDEYA, KAWAUCHI TAKAYUKI, 日本植物生理学会年会要旨集,   2007 03 15
  • 緑藻クラミドモナスのゲノム構造解析と完全長cDNA配列解析, SATAKE TOMOYA, YAMANO TAKASHI, TOYODA ATSUSHI, NARITA TAKANORI, SHIN'I TADASU, KUROKI YOKO, SUZUKI YUTAKA, ITO TAKEHIKO, SUGANO SUMIO, KOHARA YUJI, FUJIYAMA ASAO, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, 生化学,   2007
  • ゼニゴケゲノムにはDNAメチルトランスフェラーゼドメインをコードするLTR‐レトロトランスポゾン様の転移因子が存在する, YAMATO KATSUYUKI, HAMAKI ARATA, TSUCHIMOTO SUGURU, FUKUZAWA HIDEYA, KOUCHI TAKAYUKI, 生化学,   2007
  • 緑藻クラミドモナスの脂肪酸不飽和化酵素を用いた高度不飽和脂肪酸ピノレン酸のタバコにおける生産, KAJIKAWA MASATAKA, MATSUI KEISUKE, YAMATO KATSUYUKI, TANAKA YOSHIKAZU, FUKUZAWA HIDEYA, 日本農芸化学会大会講演要旨集,   2006 03 05
  • ゼニゴケゲノムにはDNAメチルトランスフェラーゼドメインをもつ新規転移因子が存在する, YAMATO KATSUYUKI, YAMAKI SHIN, FUKUZAWA HIDEYA, KAWACHI TAKAYUKI, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2006 03 05
  • ゼニゴケのアラキドン酸およびエイコサペンタエン酸に対する鎖長延長酵素遺伝子の単離と機能解析, KAJIKAWA MASATAKA, YAMATO MASAYUKI, SAKAI YASUTAKA, OYAMA KANJI, FUKUZAWA SHUYA, KAWAUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2006 03 05
  • 比較ゲノム解析に向けた緑藻クラミドモナスのゲノム情報の確立, YAMANO TAKASHI, SATAKE TOMOYA, KOZU YOSHITO, SUZUKI YUZURU, SUGANO SUMIO, FUJIYAMA ASAO, YAMATO KATSUYUKI, KAWACHI TAKAYUKI, FUKUZAWA HIDEYA, 日本植物生理学会年会要旨集,   2006 03
  • 苔類ゼニゴケにおけるフィトクロムと光形態形成の解析, KATAOKA HIDEO, MURAMOTO TAKUYA, YAMATO KATSUYUKI, KAWACHI TAKAYUKI, 日本植物生理学会年会要旨集,   2006 03
  • ゼニゴケ培養細胞を用いた高効率プラスチド形質転換系, CHIYODA MASAHIRO, LINLEY PHILIP J, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, YOKOTA AKIO, KAWACHI TAKAYUKI, 日本農芸化学会関西支部講演会講演要旨集,   2006
  • 苔類ゼニゴケフィトクロムの遺伝子同定と大腸菌再構成系を用いた分光学的解析, KATAOKA HIDEO, MUKAIGAWA YOSHIKO, KOMAI NOBUHIRO, YAMATO KATSUYUKI, KAWACHI TAKAYUKI, 日本農芸化学会関西支部講演会講演要旨集,   2006
  • ゼニゴケY染色体の構造と進化, YAMAKI ARATA, YAMATO KATSUYUKI, YODOYA KOHEI, BANDO HIROKI, KAJIKAWA MASATAKA, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, SHIN'I TADASU, KOHARA YUJI, KOUCHI TAKAYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本分子生物学会年会講演要旨集,   2005 11 25
  • ゼニゴケY染色体の構造, YAMATO KATSUYUKI, YAMAKI SHIN, YODOYA KOHEI, BANDO HIROKI, KAJIKAWA MASATAKA, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, ARAI OSAMU, OBARA YUJI, KAWAUCHI TAKAYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2005 03 05
  • ゼニゴケ由来酵素遺伝子の導入による高度不飽和脂肪酸生産タバコの創出, KAJIKAWA MASATAKA, MATSUI KEISUKE, TANAKA YOSHIKAZU, YAMATO KATSUYUKI, OYAMA KANJI, FUKUZAWA HIDEYA, KAWAUCHI TAKAYUKI, 日本農芸化学会大会講演要旨集,   2005 03 05
  • ゼニゴケY染色体のドラフト塩基配列, OYAMA KANJI, YAMAKI ARATA, YAMOTO KATSUYUKI, YODOYA KOHEI, BANDO HIROKI, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, SHIN'I TADASU, KOHARA YUJI, 日本分子生物学会年会プログラム・講演要旨集,   2004 11 25
  • 緑藻クラミドモナスのピノレン酸生合成を担うω13不飽和化酵素遺伝子の単離と機能解析, KAJIKAWA MASATAKA, YAMATO KATSUYUKI, KOZU YOSHITO, SHOJI SHIN'ICHIRO, SAKAI YASUYOSHI, FUKUZAWA HIDEYA, 日本分子生物学会年会プログラム・講演要旨集,   2004 11 25
  • 生殖器官を恒常的に形成するゼニゴケ変異体の単離と解析, YAMAOKA SHOHEI, TAKENAKA MIZUKI, HANAJIRI TSUTOMU, SHIMIZU(UEDA) YU, NISHIDA HIROYUKI, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本植物生理学会年会要旨集,   2004 03 20
  • ゼニゴケY染色体の構造解析:ドラフト塩基配列の取得と解析, OYAMA KANJI, YAMATO KATSUYUKI, YODOYA KOHEI, BANDO HIROKI, YAMAKI SHIN, ISHIZAKI KIMIYASU, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, ARAI OSAMU, 日本農芸化学会大会講演要旨集,   2004 03 05
  • ゼニゴケY染色体の構造解析:Y染色体DNAマーカーrsm40を含む530kbの遺伝子領域の推定, YAMAKI SHIN, YODOYA KOHEI, BANDO HIROKI, YAMATO KATSUYUKI, FUKUZAWA HIDETOSHI, ARAI OSAMU, KOHARA YUJI, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2004 03 05
  • ゼニゴケ高度不飽和脂肪酸生合成経路のメタノール資化性酵母Pichia pastorisでの再構成, KAJIKAWA MASATAKA, YAMATO KATSUYUKI, KOZU YOSHITO, SHOJI SHIN'ICHIRO, NOJIRI MASUTOSHI, SAKURAYA HIDEHARU, SHIMIZU MASASHI, SAKAI YASUNORI, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2004 03 05
  • 高等植物の葉の極性伸長制御遺伝子ANGUSTIFOLIAのゼニゴケ(Marchantia polymorpha)における相同遺伝子MpANの単離と解析, UENO HANAKO, KIM G-T, TSUKATANI YUICHI, KAJIKAWA MASATAKA, YAMATO KATSUYUKI, OYAMA KANJI, ONO KANJI, TAKANO HIROYOSHI, 日本植物生理学会年会要旨集,   2002 03 20
  • クラミドモナスcDNAマクロアレイを用いたCO2シグナル伝達因子CCM1標的遺伝子の検索, MIURA KENJI, KOHINATA TSUTOMU, YOSHIOKA SATOSHI, ASAMISU ERIKA, NAKAMURA YASUICHI, TABATA TETSUYUKI, YAMATO KATSUYUKI, OYAMA KANJI, FUKUZAWA HIDEYA, 日本植物生理学会年会要旨集,   2002 03 20
  • ゼニゴケの性染色体のゲノム解析 XI X色染体特異的クローンpMF28‐62B12にコードされる遺伝子, ISHIZAKI KIMITSUNE, SAKATA RYOKO, FUJISAWA MASAKI, HAYASHI KIWAKO, NISHIO TOMOHISA, BANDO HIROKI, YODOYA KOHEI, YAMATO KATSUYUKI, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2002 03 05
  • ゼニゴケ性染色体のゲノム解析 X X染色体特異的rDNAクラスターの構造解析, FUJISAWA MASAKI, NISHIO TOMOHISA, NAKAYAMA SHIGEKI, FUJISHITA MARIKO, HAYASHI KIWAKO, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2002 03 05
  • クラミドモナスcDNAマクロアレイを用いたCO2濃度変化に応答する遺伝子の網羅的解析, FUKUZAWA HIDEYA, MIURA KENJI, KOHINATA TSUTOMU, NOOKA SATOSHI, ASAMIZU ERIKA, NAKAMURA YASUKAZU, TABATA HIROYUKI, YAMATO KATSUYUKI, OYAMA KANJI, 日本農芸化学会大会講演要旨集,   2002 03 05
  • 植物進化における受容体型プロテインキナーゼ遺伝子族の多様化, SASAKI TSUYOSHI, KATO KAZUTAKA, IWABE NAOYUKI, YAMATO KATSUYUKI, OYAMA KANJI, MIYATA TAKASHI, 日本遺伝学会大会プログラム・予稿集,   2001 08 22
  • ゼニゴケの性の分化・決定機構に関する研究 IV RDA法を用いたXおよびY染色体の構造解析, FUJISAWA MASAKI, NAKAYAMA SHIGEKI, HAYASHI KIWAKO, FUJISHITA MARIKO, OKADA SHOKO, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本農芸化学会誌,   2001 03 05
  • ゼニゴケの性の分化・決定機構に関する研究 V PACクローンpMM2D3にコードされる遺伝子群, ISHIZAKI KIMIYASU, UEDA MOMEN, OKADA SHOKO, YASUDA TOMOHARU, BANDO MICHIYUKI, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本農芸化学会誌,   2001 03 05
  • ゼニゴケY染色体由来PACクローンpMM2D3にコードされる遺伝子群, ISHIZAKI KIMITSUNE, YAMATO KATSUYUKI, OKADA SACHIKO, FUJISAWA MASAKI, UEDA YU, BANDO TOMOYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本分子生物学会年会プログラム・講演要旨集,   2000 11 25
  • ゼニゴケのRLK(Receptor Like protein Kinase)遺伝子族に属する遺伝子の単離と解析, SASAKI GO, IWABE NAOYUKI, YAMATO KATSUYUKI, OYAMA KANJI, MIYATA TAKASHI, 日本分子生物学会年会プログラム・講演要旨集,   1999 11 22
  • パーティクルガンを用いたゼニゴケ葉状体の形質転換系の確立, TAKENAKA MIZUKI, YAMAOKA SHOHEI, HANAJIRI TSUTOMU, SHIMIZU YU, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本分子生物学会年会プログラム・講演要旨集,   1999 11 22
  • Genome analysis of a liverwort, Marchantia polymorpha -Expressed sequence tags from immature female sex organ, YAMATO Katsuyuki T, NAGAI Jun-ichi, SAKAIDA Megumi, FUKUZAWA Hideya, OHYAMA Kanji, 日本分子生物学会年会プログラム・講演要旨集,   1998 12 01
  • In contrast to the higher land plants, the 55 rDNA and the major rDNA of the lower land plant, liverwort Marchantia polymorpha are encoded in the same repeating units, SONE Takefumi, FUJISAWA Masaki, TAKENAKA Mizuki, NAKAGAWA Saiko, YAMATO Katsuyuki T, FUKUZAWA Hideya, OHYAMA Kanji, 日本分子生物学会年会プログラム・講演要旨集,   1998 12 01
  • ゼニゴケゲノム解析 未熟雌性生殖器官由来ESTの解析, YAMATO KATSUYUKI, NAGAI JUN'ICHI, SAKAIDA MEGUMI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本分子生物学会年会プログラム・講演要旨集,   1998 11
  • 高等陸上植物と異なり下等陸上植物の苔類ゼニゴケ(Marchantia polymorpha)では,5S rDNAと18S‐5.8S‐26S rDNAが同一繰り返し配列内に存在する, SONE TAKEFUMI, FUJISAWA MASAKI, TAKENAKA MIZUKI, NAKAGAWA SAIKO, YAMATO KATSUYUKI, FUKUZAWA HIDEYA, OYAMA KANJI, 日本分子生物学会年会プログラム・講演要旨集,   1998 11
  • Ribosomal protein genes of rice mitochondria are not organized as gene clusters., 石川繭子, 門脇光一, 大和勝幸, 大山莞爾, 中園幹生, 平井篤志, 内藤忠雄, 原田久也, Jpn J Breed,   1993
  • Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants, Plant Molecular Biology,   1999

Misc

  • ゼニゴケ(Marchantia polymorpha)の精子誘引物質の探索, 山崎由美子, 竹村美保, 松川哲也, 大和勝幸, 梶山慎一郎, 日本農芸化学会大会講演要旨集(Web), 2019, ROMBUNNO.4D5a10 (WEB ONLY),   2019 03 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902255879211422
  • Genome analysis for the liverwort Marchantia polymorpha and the diatom Nitzschia sp. NIES4239, MOCHIZUKI Takako, TANIZAWA Yasuhiro, YAMAOKA Shohei, NISHIHAMA Ryuichi, KANAZAWA Takehiko, MONTGOMERY Sean A, LIU Chang, GALIK Bence, BERGER Frederic, UEDA Takashi, YAMATO Katsuyuki T, KOHCHI Takayuki, TANIFUJI Goro, KAMIKAWA Ryoma, NAKAMURA Yasukazu, 日本植物生理学会年会(Web), 60th, 896 (WEB ONLY),   2019 , http://jglobal.jst.go.jp/public/201902246465090712
  • 苔類ゼニゴケにおけるX染色体上の性決定遺伝子の探索, 岩崎美雪, 山岡尚平, 梶原智明, 宮崎基, 末次憲之, 吉竹良洋, 西浜竜一, 大和勝幸, 河内孝之, 日本植物生理学会年会(Web), 60th, 465 (WEB ONLY),   2019 , http://jglobal.jst.go.jp/public/201902291148485936
  • ゼニゴケゲノムに対するoff targetが少ないgRNAの網羅的な自動設計と遺伝子破壊技術の構築, 田井雅人, 石野江太郎, 大和勝幸, 西浜竜一, 河内孝之, 深尾陽一朗, 菅野茂夫, 菅野茂夫, 日本植物学会大会研究発表記録, 82nd, 206,   2018 09 01 , http://jglobal.jst.go.jp/public/201802220237313075
  • ゼニゴケにおけるDNAメチル化制御, 池田陽子, 西浜竜一, 山岡尚平, ARTEAGA‐VAZQUEZ Mario. A, GRIMANELLI Daniel, MARTIENSSEN Robert A, POGORELCNIK Romain, MATHIEU Olivier, 大和勝幸, 河内孝之, 平山隆志, 日本植物学会大会研究発表記録, 82nd, 199,   2018 09 01 , http://jglobal.jst.go.jp/public/201802253353412712
  • 陸上植物の生殖細胞分化に必要な転写因子BONOBOの同定と標的遺伝子の探索, 山岡尚平, 西浜竜一, 吉竹良洋, 石田咲子, 井上佳祐, 齋藤美咲, 岡橋啓太郎, 包昊南, 西田浩之, 山口勝司, 重信秀治, 石崎公庸, 大和勝幸, 河内孝之, 日本植物学会大会研究発表記録, 82nd, 152,   2018 09 01 , http://jglobal.jst.go.jp/public/201802276371481189
  • CRISPR/Cas9を用いたJatropha curcas L.のゲノム編集の試み, 藤波友樹, 児玉岳, 十川太輔, 松川哲也, 大和勝幸, 梶山慎一郎, 日本農芸化学会大会講演要旨集(Web), 2018, ROMBUNNO.3A28a13 (WEB ONLY),   2018 03 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802229878554746
  • The transcription factor BONOBO controls sexual organ development in the basal land plant Marchantia polymorpha, Shohei Yamaoka, Keisuke Inoue, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, 50th Annual Meeting of the Japanese Society of Developmental Biologists,   2017 05
  • ゼニゴケX染色体に存在するREPRESSOR OF SILENCING 1ホモログMpROS1Xの機能解析, 十川太輔, 原田大士朗, 池田陽子, 塚本成幸, 石崎公庸, 丹羽優喜, 荒木崇, 山口勝司, 重信秀治, 河内孝之, 大和勝幸, 日本植物学会大会研究発表記録, 80th, 231,   2016 09 01 , http://jglobal.jst.go.jp/public/201602217217302097
  • The transcription factor BONOBO plays a central role in transition from vegetative to reproductive growth in the liverwort Marchantia polymorpha, Shohei Yamaoka, Keisuke Inoue, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, EMBO Workshop New model systems for early land plant evolution,   2016 06
  • 苔類ゼニゴケの成長相制御因子BONOBOの日長・光質による発現制御, 山岡尚平, 井上佳祐, 友金寛和, 西浜竜一, 山口勝司, 重信秀治, 石崎公庸, 大和勝幸, 河内孝之, 第57回日本植物生理学会年会,   2016 03
  • The transcription factor BONOBO regulates sexual organ development in the liverwort Marchantia polymorpha., Shohei Yamaoka, Hirokazu Tomogane, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi, The 2nd International Symposium on Plant Environmental Sensing,   2015 03
  • The transcription factor BONOBO appears to regulate gametangiophore formation in Marchantia polymorpha., Shohei Yamaoka, Kimitsune Ishizaki, Katsuyuki T. Yamato, Ryuichi Nishihama, Takayuki Kohchi, Marchantia Workshop 2014,   2014 12 , 招待有り
  • 原核生物型PEBPファミリータンパク質CORのシロイヌナズナとゼニゴケにおける機能解析, 森花小百合, 竹本覚, 辻井由香, 有手友嗣, 竹村美保, 酒井友希, 山口礼子, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物生理学会年会要旨集, 54th, 131,   2013 03 14 , http://jglobal.jst.go.jp/public/201302248860148435
  • シロイヌナズナのホルムアルデヒドストレス応答と活性酸素種(ROS)の関与に関する解析, 久保森, 榊原均, 来須孝光, 朽津和幸, 由理本博也, 阪井康能, 秋田求, 大和勝幸, 泉井桂, 日本植物生理学会年会要旨集, 53rd, 192,   2012 03 09 , http://jglobal.jst.go.jp/public/201202232539389233
  • イネへのホルムアルデヒドの同化代謝系(リブロースモノリン酸経路)の導入, 鈴木詩織, 明渡絵里朱, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 29th, 148,   2011 09 06 , http://jglobal.jst.go.jp/public/201102254893180968
  • リブロースモノリン酸経路(RuMP)の2種類の酵素の融合体をコードする合成遺伝子を導入したイネの作成と解析, 鈴木詩織, 明渡絵里朱, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 日本農芸化学会大会講演要旨集, 2011, 9,   2011 03 05 , http://jglobal.jst.go.jp/public/201102281041215630
  • イネとタバコへのホルムアルデヒド固定能の付与:C1微生物のリブロースモノリン酸経路(RuMP)の2つの酵素の葉緑体における高発現にむけて, 明渡絵里朱, 鈴木詩織, 福井崇人, 中川強, 榊原均, 由里本博也, 阪井康能, 大和勝幸, 秋田求, 泉井桂, 生化学, ROMBUNNO.2P-0223,   2011 , http://jglobal.jst.go.jp/public/201202286720615620
  • 苔類ゼニゴケにおけるPEBP family遺伝子の機能解析, 竹本覚, 辻井由香, 有手友嗣, 竹村美保, 酒井友希, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物学会大会研究発表記録, 74th, 175,   2010 09 08 , http://jglobal.jst.go.jp/public/201002222103652591
  • 苔類ゼニゴケにおけるLEAFY相同遺伝子MpLFYの機能解析, 酒井友希, 宮下結衣, 宇山和樹, 辻井由香, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物学会大会研究発表記録, 74th, 175,   2010 09 08 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002289451990783
  • ゼニゴケFLO/LFY相同遺伝子の機能解析, 宇山和樹, 宮下結衣, 辻井由香, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 福澤秀哉, 河内孝之, 荒木崇, 日本植物生理学会年会要旨集, 51st, 216,   2010 03 12 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002275018824495
  • ゼニゴケFLO/LFY相同遺伝子の機能解析, 宇山和樹, 辻井由香, 宮下結衣, 大門靖史, 遠藤求, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物学会大会研究発表記録, 73rd, 99,   2009 09 17 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902234436459974
  • ゼニゴケFLO/LFY相同遺伝子の単離と解析, 辻井由香, 宇山和樹, 大門靖史, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物生理学会年会要旨集, 50th, 237,   2009 03 16 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902294511641182
  • The liverwort Marchantia polymorpha as an emerging model plant, Katsuyuki T. Yamato, Kimitsune Ishizaki, Aino Komatsu, Akane Kubota, Memorial Symposium for the 25th International Prize for Biology Celebrating Dr. Winslow R. Briggs,   2009
  • ゼニゴケFLO/LFY相同遺伝子の単離と解析, 辻井由香, 大門靖史, 石崎公庸, 大和勝幸, 河内孝之, 荒木崇, 日本植物学会大会研究発表記録, 72nd, 157,   2008 09 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902206605706273
  • 緑藻クラミドモナスの脂肪酸不飽和化酵素を用いた高度不飽和脂肪酸ピノレン酸のタバコにおける生産, 梶川昌孝, 松井啓祐, 大和勝幸, 田中良和, 福澤秀哉, 日本農芸化学会大会講演要旨集, 2006, 187,   2006 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902247085552705
  • ゼニゴケのアラキドン酸およびエイコサペンタエン酸に対する鎖長延長酵素遺伝子の単離と機能解析, 梶川昌孝, 大和勝幸, 阪井康能, 大山莞爾, 福澤秀哉, 河内孝之, 日本農芸化学会大会講演要旨集, 2006, 33,   2006 03 05 , http://jglobal.jst.go.jp/public/200902227612572344
  • ゼニゴケY染色体の構造と進化, 八巻新, 大和勝幸, 淀谷幸平, 坂東弘樹, 梶川昌孝, 中山繁樹, 藤下まり子, 新井理, 小原雄治, 河内孝之, 福沢秀哉, 大山莞爾, 日本分子生物学会年会講演要旨集, 28th, 159,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902276267059141
  • ゼニゴケ由来酵素遺伝子の導入による高度不飽和脂肪酸生産タバコの創出, 梶川昌孝, 松井啓祐, 田中良和, 大和勝幸, 大山莞爾, 福沢秀哉, 河内孝之, 日本農芸化学会大会講演要旨集, 2005, 205,   2005 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902235061783155
  • 緑藻クラミドモナスのピノレン酸生合成を担うω13不飽和化酵素遺伝子の単離と機能解析, 梶川昌孝, 大和勝幸, 甲津嘉人, 庄司信一郎, 阪井康能, 福沢秀哉, 日本分子生物学会年会プログラム・講演要旨集, 27th, 850,   2004 11 25 , http://jglobal.jst.go.jp/public/200902229099353260
  • 生殖器官を恒常的に形成するゼニゴケ変異体の単離と解析, 山岡尚平, 竹中瑞樹, 葉名尻勤, 清水(上田)木綿, 西田浩之, 大和勝幸, 福沢秀哉, 大山莞爾, 日本植物生理学会年会要旨集, 45th, 146,   2004 03 20 , 10.14841/jspp.2004.0.183.0, http://jglobal.jst.go.jp/public/200902278032954871
  • ゼニゴケ高度不飽和脂肪酸生合成経路のメタノール資化性酵母Pichia pastorisでの再構成, 梶川昌孝, 大和勝幸, 甲津嘉人, 庄司信一郎, 野尻増俊, 桜谷英治, 清水昌, 阪井康能, 大山莞爾, 日本農芸化学会大会講演要旨集, 2004, 304,   2004 03 05 , http://jglobal.jst.go.jp/public/200902213816918435
  • 高等植物の葉の極性伸長制御遺伝子ANGUSTIFOLIAのゼニゴケ(Marchantia polymorpha)における相同遺伝子MpANの単離と解析, 上野華子, KIM G‐T, 塚谷裕一, 梶川昌孝, 大和勝幸, 大山莞爾, 小野莞爾, 高野博嘉, 日本植物生理学会年会要旨集, 42nd, 184,   2002 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902177723337665
  • パーティクルガンを用いたゼニゴケ葉状体の形質転換系の確立, 竹中瑞樹, 山岡尚平, 葉名尻勤, 清水木綿, 大和勝幸, 福沢秀哉, 大山莞爾, 日本分子生物学会年会プログラム・講演要旨集, 22nd, 761,   1999 11 22 , http://jglobal.jst.go.jp/public/200902151506509530
  • Development of a novel selectable marker for liverwort (Marchantia polymorpha)., Ueda M, Ishizaki K, Yamato K. T, Kohchi T, Shikanai T, Nishimura Y, Marchantia workshop 2010, March 11-12, 2010, Kyoto, Japan,   2010 , Refereed
  • Novel transposable element in the liverwort Marchantia polymorpha L. contains DNA methyltransferase domain, Katsuyuki Yamato, Arata Yamaki, Suguru Tsuchimoto, Hideya Fukuzawa, Takayuki Kohchi, PLANT AND CELL PHYSIOLOGY, 48, S255, S255,   2007
  • Distinct differentiation of sex chromosomes in liverwort (Marchantia polymorpha L.), Shigeki Nakayama, Katsuyuki T. Yamato, Mariko Fujishita, Kanji Ohyama, CHROMOSOME RESEARCH, 15, 78, 78,   2007
  • Functional and photomorphogenic analysis of phytochrome in Marchantia polymorpha, H Kataoka, T Muramoto, KT Yamato, T Kohchi, PLANT AND CELL PHYSIOLOGY, 47, S204, S204,   2006
  • Establishment of genome resource of Chlamydomonas reinhardtii toward comparative genomics of photosynthetic organisms, T Yamano, T Satake, Y Kohzu, Y Suzuki, S Sugano, A Fujiyama, K Yamato, T Kohchi, H Fukuzawa, PLANT AND CELL PHYSIOLOGY, 47, S245, S245,   2006
  • A mutant with constitutive sexual organ development in Marchantia polymorpha L., S Yamaoka, M Takenaka, T Hanajiri, Y Shimizu-Ueda, H Nishida, K Yamato, H Fukuzawa, K Ohyama, PLANT AND CELL PHYSIOLOGY, 45, S73, S73,   2004
  • Gene organization of the Y chromosome of the liverwort, Marchantia polymorpha, K Oyama, KT Yamato, K Ishizaki, S Okada, M Fujisawa, S Nakayama, M Fujishita, T Bando, A Hasumi, R Sakata, T Nishio, K Yodoya, H Bando, PLANT AND CELL PHYSIOLOGY, 44, S29, S29,   2003
  • Relationship between CO2-limiting stress and high-light stress in a green alga, Chlamydomonas reinhardtii deduced from cDNA array analyses, K Miura, T Yamano, T Kohinata, S Yoshioka, E Shimada, J Minagawa, T Seguchi, E Asamizu, Y Nakamura, S Tabata, K Yamato, K Ohyama, H Fukuzawa, PLANT AND CELL PHYSIOLOGY, 44, S180, S180,   2003
  • Isolation and characterization of MpAN gene, which is homologous to Arabidopsis ANGUSTIFOLIA, from liverwort (Marchantia polymorpha), H Ueno, GT Kim, H Tsukaya, M Kajikawa, K Yamato, K Ohyama, K Ono, H Takano, PLANT AND CELL PHYSIOLOGY, 43, S138, S138,   2002
  • Identification of genes transcriptionally regulated by the Ccm1 gene by using Chamydomonas CDNA macroarray, K Miura, T Kohinata, S Yoshioka, E Asamizu, Y Nakamura, S Tabata, K Yamato, K Ohyama, H Fukuzawa, PLANT AND CELL PHYSIOLOGY, 43, S204, S204,   2002

Awards & Honors

  •   2013 09 , the Botanical Society of Japan, Best Paper Award 2013, Visualization of auxin-mediated transcriptional activation using a common auxin-responsive reporter system in the liverwort Marchantia polymorpha.

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Evolution of sex chromosomes in haploid genome, Among the genes identified in the Y chromosomal region under investigation, eight appears to have essential functions and thus are expected to have their homologs on the X chromosome. In fact, the Y-chromosomal M547D3.1 gene has its X-chromosomal partner M547D3.1F. Additional 39 X-chromosomal counterparts were obtained from female genomic data provided from JGI.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Functional analysis on sex-chromosome genes in haploid organism, On the Y chromosome of the liverwort, Marchantia polymorpha L., 64 genes are identified, 14 of which are detected only in the male genome. These 14 genes are expressed in reproductive organs but not in vegetative thalli, suggesting their participation in male reproductive functions. The aim of this project is to prove this hypothesis.For all but one of the 14 genes, cDNA sequences were determined by RACE, and their promoter regions were predicted by comparing the cDNA sequences and genomic sequences. To facilitate transgenic analyses, we have developed a rapid Agrobacterium-mediated transformation system for M. polymorpha using immature thalli developed from spores (Ishizaki, et. al., submitted). First, the M350E4.4 gene, which encodes F-box protein related to Arabidopsis UFO, was selected as a model case. To examine its expression pattern, a construct which carries the predicted promoter region of M350E4.4 and the β -glucuronidase gene (GUS) was introduced to immature thalli. No GUS activity was detected in transgenic thalli, which is consistent with our previous result of RT-PCR. Expression of 3135054.4 in sexual organ is under investigation. Four types of constructs were tested to examine the function of M350E4.4: (1) ectopic expression by CaMV35S promoter, (2) silencing by expressing dsRNA, (3) overexpression of F-box domain, and (4) overexpression of the coding sequence without F-box domain. Thalli of transgenic plants examined thus far were morphologically indistinguishable from wild-type. Morphology of sexual organ of transgenic plants are being investigated.
  • Structure of sex chromosomes in liverwort.