KINDAI UNIVERSITY


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AZUMA Yoshinao

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FacultyDepartment of Science and Technology on Food Safety / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
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Commentator Guidehttps://www.kindai.ac.jp/meikan/493-azuma-yoshinao.html
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Last Updated :2020/09/30

Education and Career

Academic & Professional Experience

  •   2017 04 ,  - 現在, Professor, Molecular Biochemistry Lab, Faculty of Biology-Oriented Science and Technology,, Kindai University
  •   2010 04 ,  - 2017 03 , Faculty of Biology-Oriented Science and Technology, Department of Science and Technology on Food Safety, Kindai University
  • Faculty of Agriculture, Kyoto University

Research Activities

Research Areas

  • Life sciences, Bacteriology
  • Life sciences, Evolutionary biology
  • Life sciences, Applied microbiology
  • Life sciences, Biomaterials
  • Life sciences, Biomedical engineering

Research Interests

  • 3-hydroxybutyrate, PHB, Acetobacter, Plasmodiophora, Clubroot disease, Halomonas, Acetic Acid Bacteria, Chlamydia

Published Papers

  • Identification of Chlamydia pneumoniae candidate genes that interact with human apoptotic factor caspase-9., Md. Abdul Aziz, Rie Ushirokita, Yoshinao Azuma, Journal of General and Applied Microbiology, Journal of General and Applied Microbiology, 64(5), 253 - 257, 2018 , Refereed
  • Informatical analysis of archaeal genomic DNA sequences: Gene composition of archaeal genomes., Azuma, Y, Ohfuku, Y, Kakinuma, J, Koike, H, Amano, N, Tateno, M, Suckow, JM, Kudo, N, Amano, N, Kakinuma, J, Suzuki, M, Microbial & Comparative Genomics, Microbial & Comparative Genomics, 3: 21-22, 1998 , Refereed
  • In vitro thermal adaptation of mesophilic Acetobacter pasteurianus NBRC 3283 generates thermotolerant strains with evolutionary trade-offs., Nami Matsumoto, Minenosuke Matsutani, Yoshinao Azuma, Naoya Kataoka, Toshiharu Yakushi, Kazunobu Matsushita, Bioscience, biotechnology, and biochemistry, Bioscience, biotechnology, and biochemistry, 84(4), 832 - 841, Apr. 2020 , Refereed
    Summary:Thermotolerant strains are critical for low-cost high temperature fermentation. In this study, we carried out the thermal adaptation of A. pasteurianus IFO 3283-32 under acetic acid fermentation conditions using an experimental evolution approach from 37ºC to 40ºC. The adapted strain exhibited an increased growth and acetic acid fermentation ability at high temperatures, however, with the trade-off response of the opposite phenotype at low temperatures. Genome analysis followed by PCR sequencing showed that the most adapted strain had 11 mutations, a single 64-kb large deletion, and a single plasmid loss. Comparative phenotypic analysis showed that at least the large deletion (containing many ribosomal RNAs and tRNAs genes) and a mutation of DNA polymerase (one of the 11 mutations) critically contributed to this thermotolerance. The relationship between the phenotypic changes and the gene mutations are discussed, comparing with another thermally adapted A. pasteurianus strains obtained previously.
  • Supplementation of pancreatic digestive enzymes alters the composition of intestinal microbiota in mice, Hiroki Nishiyama, Tomoyuki Nagai, Masatoshi Kudo, Yoshihisa Okazaki, Yoshinao Azuma, Tomohiro Watanabe, Susumu Goto, Hiroyuki Ogata, Toshiharu Sakurai, Biochemical and Biophysical Research Communications, Biochemical and Biophysical Research Communications, 495(1), 273 - 279, Jan. 2018 , Refereed
  • Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture, Tohru Hayakawa, Akira Sakakibara, Sho Ueda, Yoshinao Azuma, Toru Ide, So Takebe, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 87(87), 100 - 106, Aug. 2017 , Refereed
    Summary:Cry46Ab is a Cry toxin derived from Bacillus thuringiensis TK-E6. Cry46Ab is not significantly homologous to other mosquitocidal Cry or Cyt toxins and is classified as an aerolysin-type pore-forming toxin based on structural similarity. In this study, the potency of Cry46Ab was assessed for its potential application to mosquito control. A synthetic Cry46Ab gene, ciy46Ab-S1, was designed to produce recombinant Cry46Ab as a glutathione-S-transferase fusion in Escherichia coli. Recombinant Cry46Ab showed apparent toxicity to Culex pipiens larvae, with a 50% lethal dose of 1.02 mu g/ml. In an artificial lipid bilayer, Cry46Ab activated by trypsin caused typical current transitions between open and closed states, suggesting it functions as a pore-forming toxin similar to other Cry and Cyt toxins. The single-channel conductance was 103.3 +/- 4.1 pS in 150 mM KCl. Co-administration of recombinant Cry46Ab with other mosquitocidal Cry toxins, especially the combination of Cry4Aa and Cry46Ab, resulted in significant synergistic toxicity against C. pipiens larvae. Co-administration of multiple toxins exhibiting different modes of action is believed to prevent the onset of resistance in insects. Our data, taken in consideration with the differences in its structure, suggest that Cry46Ab could be useful in not only reducing resistance levels but also improving the insecticidal activity of Bt-based bio-insecticides. (C) 2017 Elsevier Ltd. All rights reserved.
  • Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations, Kazunobu Matsushita, Yoshinao Azuma, Tomoyuki Kosaka, Toshiharu Yakushi, Hisashi Hoshida, Rinji Akada, Mamoru Yamada, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(4), 655 - 668, Apr. 2016 , Refereed
  • Preparation and Characterizations of Dispersible Fluorinated Hydroxyapatite Nanoparticles with Weak Antibacterial Activity, Tsutomu Furuzono, Yoshinao Azuma, Yuichi Niigawa, Yasumichi Kogai, Yoshiki Sawa, ASAIO JOURNAL, ASAIO JOURNAL, 62(2), 197 - 202, Mar. 2016 , Refereed
    Summary:To develop a nanoscaled coating material for medical devices possessing weak antibacterial activity, dispersible and crystalline fluorinated hydroxyapatite (F-HAp) nanoparticles were prepared using antisintering agent to avoid calcination-induced sintering. The product was identical to fluorapatite, as determined by X-ray diffraction and Fourier transform infrared spectroscopy. The primary particles generally showed rod-shaped morphology with a length of 367 +/- 67 nm and a width of 223 +/- 21 nm measured by scanning electron microscopy (SEM). The dispersed average particle size (313 +/- 51 nm) in ethanol analyzed by dynamic light scattering was almost the same as that obtained from the SEM images. In the evaluation of solubility in acidic aqueous solution, F-HAp and original hydroxyapatite (HAp) nanoparticles started to dissolve at around pH 3.4 and 4.2, respectively. Thus, the stability of F-HAp in a living body increased compared with original HAp. The antibacterial activity of F-HAp nanoparticles was higher than that of fluoride in sodium fluoride alone or the original HAp nanoparticles. However, it was estimated that the effect of F-HAp was much lower compared with that of silver, one of the popular antibacterial materials. Thus, the dispersed F-HAp nanoparticles possessing weak antimicrobial activity can be useful without severe damage to the living tissue.
  • Newly Developed Biocompatible Material: Dispersible Titanium-Doped Hydroxyapatite Nanoparticles Suitable for Antibacterial Coating on Intravascular Catheters, Tsutomu Furuzono, Masatoshi Okazaki, Yoshinao Azuma, Mitsunobu Iwasaki, Yasumichi Kogai, Yoshiki Sawa, Contributions to Nephrology, Contributions to Nephrology, 189, 144 - 152, 2016 , Refereed
    Summary:Background: Thirteen patients with chlorhexidine-silver sulfadiazine-impregnated catheters have experienced serious anaphylactic shock in Japan. These adverse reactions highlight the lack of commercially available catheters impregnated with strong antibacterial chemical agents. A system should be developed that can control both biocompatibility and antibacterial activity. Summary: Hydroxyapatite (HAp) is biocompatible with bone and skin tissues. To provide antibacterial activity by using an external physical stimulus, titanium (Ti) ions were doped into the HAp structure. Highly dispersible, Ti-doped HAp (Ti-HAp) nanoparticles suitable as a coating material were developed. In 3 kinds of Ti-HAp [Ti/(Ca + Ti) = 0.05, 0.1, 0.2], the Ti content in the HAp was approximately 70% of that used in the Ti-HAp preparation, as determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES). ICP-AES and X-ray diffraction showed Ti ions were well substituted into the HAp lattice. The nanoparticles were almost uniformly coated on a polyethylene (PE) sheet in a near-monolayer with a surface coverage ratio > 95%. The antibacterial activity of the Ti-HAp nanoparticles containing 7.3% Ti ions and coating the sheet was evaluated by calculating the survival ratio of Pseudomonas aeruginosa on the coated sheet after ultraviolet (UV) irradiation. The Ti-HAp-coated sheet showed a 50% decrease in the number of P. aeruginosa compared with that on an uncoated control PE sheet after UV irradiation for 30 s. Key Messages: A system of biocompatibility and antibacterial activity with an on/off switch controlled by external UV stimulation was developed. The system is expected to be applicable in long-term implanted intravascular catheters.
  • Complete genome and gene expression analyses of Asaia bogorensis reveal unique responses to culture with mammalian cells as a potential opportunistic human pathogen, Mikihiko Kawai, Norie Higashiura, Kimie Hayasaki, Naruhei Okamoto, Akiko Takami, Hideki Hirakawa, Kazunobu Matsushita, Yoshinao Azuma, DNA RESEARCH, DNA RESEARCH, 22(5), 357 - 366, Oct. 2015 , Refereed
    Summary:Asaia bogorensis, a member of acetic acid bacteria (AAB), is an aerobic bacterium isolated from flowers and fruits, as well as an opportunistic pathogen that causes human peritonitis and bacteraemia. Here, we determined the complete genomic sequence of the As. bogorensis type strain NBRC 16594, and conducted comparative analyses of gene expression under different conditions of co-culture with mammalian cells and standard AAB culture. The genome of As. bogorensis contained 2,758 protein-coding genes within a circular chromosome of 3,198,265 bp. There were two complete operons encoding cytochrome bo(3)-type ubiquinol terminal oxidases: cyoABCD-1 and cyoABCD-2. The cyoABCD-1 operon was phylogenetically common to AAB genomes, whereas the cyoABCD-2 operon belonged to a lineage distinctive from the cyoABCD-1 operon. Interestingly, cyoABCD-1 was less expressed under co-culture conditions than under the AAB culture conditions, whereas the converse was true for cyoABCD-2. Asaia bogorensis shared pathogenesis-related genes with another pathogenic AAB, Granulibacter bethesdensis, including a gene coding pathogen-specific large bacterial adhesin and additional genes for the inhibition of oxidation and antibiotic resistance. Expression alteration of the respiratory chain and unique hypothetical genes may be key traits that enable the bacterium to survive under the co-culture conditions.
  • An epistatic effect of apaf-1 and caspase-9 on chlamydial infection, Mohd. Akhlakur Rahman, Mutsunori Shirai, Md. Abdul Aziz, Rie Ushirokita, Sayuri Kubota, Harumi Suzuki, Yoshinao Azuma, APOPTOSIS, APOPTOSIS, 20(10), 1271 - 1280, Oct. 2015 , Refereed
    Summary:Chlamydia is an obligate intracellular bacterial pathogen that replicates solely within a membrane-bound vacuole termed an inclusion. Chlamydia seems to perturb multiple cellular processes of the host, such as, rearrangement of the membrane trafficking system for its intracellular multiplication, and inhibition of host cell apoptosis for persistent infection. In an attempt to clarify host factor involvement in apoptosis regulation, we found that inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. These opposition contributions to the chlamydial infection were confirmed using caspase-9 (-/-) and apaf-1 (-/-) MEFs. Similar phenomena also appeared in the case of infection with Chlamydia trachomatis. Interestingly, caspase-9 in apaf-1 (-/-) MEFs was activated by chlamydial infection but during the infection caspase-3 was not activated. That is, caspase-9 was activated without support for multiplication and activation by Apaf-1, and the activated caspase-9 may be physically disconnected from the caspase cascade. This may be partially explained by the observation of caspase-9 accumulation within chlamydial inclusions. The sequestration of caspase-9 by chlamydia seems to result in apoptosis repression, which is crucial for the chlamydial development cycle. Because Apaf-1 shares domains with intracellular innate immune receptor NOD1, it may play a key role in the strategy to regulate chlamydial infection.
  • Adaptive mutation related to cellulose producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288, Minenosuke Matsutani, Kohei Ito, Yoshinao Azuma, Hidetaka Ogino, Mutsunori Shirai, Toshiharu Yakushi, Kazunobu Matsushita, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(17), 7229 - 7240, Sep. 2015 , Refereed
    Summary:Gluconacetobacter xylinus (formerly Acetobacter xylinum and presently Komagataeibacter medellinensis) is known to produce cellulose as a stable pellicle. However, it is also well known to lose this ability very easily. We investigated the on and off mechanisms of cellulose producibility in two independent cellulose-producing strains, R1 and R2. Both these strains were isolated through a repetitive static culture of a non-cellulose-producing K. medellinensis NBRC 3288 parental strain. Two cellulose synthase operons, types I and II, of this strain are truncated by the frameshift mutation in the bcsBI gene and transposon insertion in the bcsCII gene, respectively. The draft genome sequencing of R1 and R2 strains revealed that in both strains the bcsBI gene was restored by deletion of a nucleotide in its C-rich region. This result suggests that the mutations in the bcsBI gene are responsible for the on and off mechanism of cellulose producibility. When we looked at the genomic DNA sequences of other Komagataeibacter species, several non-cellulose-producing strains were found to contain similar defects in the type I and/or type II cellulose synthase operons. Furthermore, the phylogenetic relationship among cellulose synthase genes conserved in other bacterial species was analyzed. We observed that the cellulose genes in the Komagataeibacter shared sequence similarities with the gamma-proteobacterial species but not with the alpha-proteobacteria and that the type I and type II operons could be diverged from a same ancestor in Komagataeibacter.
  • Synthesis and antibacterial evaluation of calcinated Ag-doped nano-hydroxyapatite with dispersibility, Tsutomu Furuzono, Mazumder Motaharul, Yasumichi Kogai, Yoshinao Azuma, Yoshiki Sawa, INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 38(5), 251 - 258, May 2015 , Refereed
    Summary:Purpose: Dispersible hydroxyapatite (HAp) nanoparticles are very useful for applying a monolayer to implantable medical devices using the nano-coating technique. To improve tolerance to infection on implanted medical devices, silver-doped HAp (Ag-HAp) nanoparticles with dispersiblity and crystallinity were synthesized, avoiding calcination-induced sintering, and evaluated for antibacterial activity. Methods: The Ca10-xAgx(PO4)(6)(OH)(2) with x = 0 and 0.2 were prepared by wet chemical processing at 100 degrees C. Before calcination at 700 degrees C for 2 h, two kinds of anti-sintering agents, namely a Ca(NO3)(2) (Ca salt) and a polyacrylic acid/Ca salt mixture (PAA-Ca), were used. Escherichia coli was used to evaluate the antibacterial activity of the nanopowder. Results: When PAA-Ca was used as an anti-sintering agent in calcination to prepare the dispersible nanoparticles, strong metallic Ag peaks were observed at 38.1 degrees and 44.3 degrees (2 theta) in the X-ray diffraction (XRD) profile. However, the Ag peak was barely observed when Ca salt was used alone as the anti-sintering agent. Thus, using Ca salt alone was more effective for preparation of dispersible Ag-HAp than PAA-Ca. The particle average size of Ag-HAp with 0.5 mol% of Ag content was found to be 325 +/- 70 nm when the formation of large particle-aggregations was prevented, as determined by dynamic light scattering instrument. The antibacterial activity of the Ag-HAp nanoparticles possessing 0.5 mol% against E. coli was greater than 90.0%. Conclusions: Dispersible and crystalline nano Ag-HAp can be obtained by using Ca salt alone as an anti-sintering agent. The nanoparticles showed antibacterial activity.
  • Draft genomic DNA sequence of the facultatively methylotrophic bacterium Acidomonas methanolica type strain MB58, Norie Higashiura, Hiromi Hadano, Hideki Hirakawa, Minenosuke Matsutani, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, FEMS MICROBIOLOGY LETTERS, FEMS MICROBIOLOGY LETTERS, 351(1), 9 - 13, Feb. 2014 , Refereed
    Summary:Acidomonas methanolica (former name: Acetobacter methanolicus) is a unique acetic acid bacterium capable of growing on methanol as a sole carbon source. We reported the draft genome sequencing of A.methanolica type strain MB58, showing that it contains 3270 protein-coding genes, including the genes involved in oxidation of methanol, such as mxaFJGIRSACKL and hxlAB, and oxidation of ethanol, such as adhAB and adhS.
  • Preparation of carboxylated Ag nanoparticles as a coating material for medical devices and control of antibacterial activity, Tsutomu Furuzono, Takashi Iwamoto, Yoshinao Azuma, Masahiro Okada, Yoshiki Sawa, JOURNAL OF ARTIFICIAL ORGANS, JOURNAL OF ARTIFICIAL ORGANS, 16(4), 451 - 457, Dec. 2013 , Refereed
    Summary:Carboxyl group-donated silver (Ag) nanoparticles for coating on medical devices were prepared by a two-phase reduction system in situ. AgNO3 was the Ag ion source, tetraoctylammonium bromide [N(C8H17)(4)Br] the phase-transfer agent, sodium tetrahydroborate (NaBH4) the reducing agent and 10-carboxy-1-decanthiol (C11H22O2S, CDT) the capping agent. The characterizations of the Ag nanoparticles were conducted by diffuse reflectance Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric differential thermal analysis (TG/DTA) and transmission electron microscope. With CDT capped on Ag nanoparticles, we found that the band around 3,100 cm(-1) was attributed to COO-H stretching vibration, two adsorptions at 2,928 and 2,856 cm(-1) to C-H symmetric/anti-symmetric stretching vibration, and at 1,718 cm(-1) to C=O stretching vibration in the FT-IR spectra. The organic components of the carboxylated Ag nanoparticles were 5.8-25.9 wt%, determined by TG/DTA. The particle sizes of the carboxylated Ag nanoparticles were well controlled by the addition of the capping agent, CDT, into the reaction system. The antimicrobial activity of the Ag nanoparticles covered with different contents of CDT against E. coli was evaluated. Smaller-size Ag nanoparticles showed higher antibacterial activity, which depended on a surface area that attached easily to a microorganism cell membrane.
  • Complete Genomic DNA Sequence of the East Asian Spotted Fever Disease Agent Rickettsia japonica, Minenosuke Matsutani, Motohiko Ogawa, Naohisa Takaoka, Nozomu Hanaoka, Hidehiro Toh, Atsushi Yamashita, Kenshiro Oshima, Hideki Hirakawa, Satoru Kuhara, Harumi Suzuki, Masahira Hattori, Toshio Kishimoto, Shuji Ando, Yoshinao Azuma, Mutsunori Shirai, PLOS ONE, PLOS ONE, 8(9), e71861, Sep. 2013 , Refereed
    Summary:Rickettsia japonica is an obligate intracellular alphaproteobacteria that causes tick-borne Japanese spotted fever, which has spread throughout East Asia. We determined the complete genomic DNA sequence of R. japonica type strain YH (VR-1363), which consists of 1,283,087 base pairs (bp) and 971 protein-coding genes. Comparison of the genomic DNA sequence of R. japonica with other rickettsiae in the public databases showed that 2 regions (4,323 and 216 bp) were conserved in a very narrow range of Rickettsia species, and the shorter one was inserted in, and disrupted, a preexisting open reading frame (ORF). While it is unknown how the DNA sequences were acquired in R. japonica genomes, it may be a useful signature for the diagnosis of Rickettsia species. Instead of the species-specific inserted DNA sequences, rickettsial genomes contain Rickettsia-specific palindromic elements (RPEs), which are also capable of locating in preexisting ORFs. Precise alignments of protein and DNA sequences involving RPEs showed that when a gene contains an inserted DNA sequence, each rickettsial ortholog carried an inserted DNA sequence at the same locus. The sequence, ATGAC, was shown to be highly frequent and thus characteristic in certain RPEs (RPE-4, RPE-6, and RPE-7). This finding implies that RPE-4, RPE-6, and RPE-7 were derived from a common inserted DNA sequence.
  • Complete Genome Sequence of NBRC 3288, a Unique Cellulose-Nonproducing Strain of Gluconacetobacter xylinus Isolated from Vinegar, Hidetaka Ogino, Yoshinao Azuma, Akira Hosoyama, Hidekazu Nakazawa, Minenosuke Matsutani, Akihiro Hasegawa, Ken-ichiro Otsuyama, Kazunobu Matsushita, Nobuyuki Fujita, Mutsunori Shirai, JOURNAL OF BACTERIOLOGY, JOURNAL OF BACTERIOLOGY, 193(24), 6997 - 6998, Dec. 2011 , Refereed
    Summary:Gluconacetobacter xylinus is involved in the industrial production of cellulose. We have determined the genome sequence of G. xylinus NBRC 3288, a cellulose-nonproducing strain. Comparative analysis of genomes of G. xylinus NBRC 3288 with those of the cellulose-producing strains clarified the genes important for cellulose production in Gluconacetobacter.
  • Diagnostic Assay for Rickettsia japonica, Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, Shuji Ando, EMERGING INFECTIOUS DISEASES, EMERGING INFECTIOUS DISEASES, 15(12), 1994 - 1997, Dec. 2009 , Refereed
    Summary:We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica-related strains.
  • Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus, Yoshinao Azuma, Akira Hosoyama, Minenosuke Matsutani, Naoko Furuya, Hiroshi Horikawa, Takeshi Harada, Hideki Hirakawa, Satoru Kuhara, Kazunobu Matsushita, Nobuyuki Fujita, Mutsunori Shirai, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 37(17), 5768 - 5783, Sep. 2009 , Refereed
    Summary:Acetobacter species have been used for brewing traditional vinegar and are known to have genetic instability. To clarify the mutability, Acetobacter pasteurianus NBRC 3283, which forms a multiphenotype cell complex, was subjected to genome DNA sequencing. The genome analysis revealed that there are more than 280 transposons and five genes with hyper-mutable tandem repeats as common features in the genome consisting of a 2.9-Mb chromosome and six plasmids. There were three single nucleotide mutations and five transposon insertions in 32 isolates from the cell complex. The A. pasteurianus hyper-mutability was applied for breeding a temperature-resistant strain grown at an unviable high-temperature (42 degrees C). The genomic DNA sequence of a heritable mutant showing temperature resistance was analyzed by mutation mapping, illustrating that a 92-kappa b deletion and three single nucleotide mutations occurred in the genome during the adaptation. Alpha-proteobacteria including A. pasteurianus consists of many intracellular symbionts and parasites, and their genomes show increased evolution rates and intensive genome reduction. However, A. pasteurianus is assumed to be a free-living bacterium, it may have the potentiality to evolve to fit in natural niches of seasonal fruits and flowers with other organisms, such as yeasts and lactic acid bacteria.
  • Rac GTPases are involved in development, survival and homeostasis of T cells, Yoshinori Sato, Hiroyo Oda, Michael S. Patrick, Yukari Baba, Ahmed A. Rus'd, Yoshinao Azuma, Takaya Abe, Mutsunori Shirai, Harumi Suzuki, IMMUNOLOGY LETTERS, IMMUNOLOGY LETTERS, 124(1), 27 - 34, May 2009 , Refereed
    Summary:Rac GTPases consist of Rac1, 2 and 3, and each of them have redundant and differential functions. Rac1 is the most ubiquitously and abundantly expressed of the three and has been shown to work as a "molecular switch" in various signal transduction pathways. Although Rac1 and Rac2 are both activated by TCR ligation, little is known about the function of Rac GTPases in the development and activation of T cells. In order to investigate the precise function of Rac GTPases in T cells in vivo, we established dominant negative Rac1 transgenic (dnRac1-Tg) mice controlled by the human CD2 promoter. Total numbers of thymocytes of dnRac1-Tg mice were significantly decreased because of impaired transition from the CD4CD8 double negative stage to the CD4CD8 double positive (DP) stage. Although positive selection of CD4 single positive (SP) was not altered, positive selection of CD8-SP was slightly increased. On the contrary, the number of mature CD4-SP and CD8-SP cells in the spleen, mesenteric lymph nodes and peripheral blood was severely decreased in dnRac1-Tg mice. Proliferation of splenic CD4-SP cells upon TCR stimulation in vitro was unaltered, however, homeostatic proliferation of dnRac1-Tg splenic CD4-SP cells in lymphopenic mice was severely reduced. Finally, we found increased spontaneous apoptosis of DP thymocytes and mature T cells in dnRac1-Tg mice, possibly because of reduced phosphorylation of Akt with or without TCR stimulation. Collectively, the current results indicate that Rac GTPases are important in survival of DP thymocytes and mature T cells in vivo by regulating Akt activation. (c) 2009 Elsevier B.V. All rights reserved.
  • RhoH Plays Critical Roles in Fc epsilon RI-Dependent Signal Transduction in Mast Cells, Hiroyo Oda, Manabu Fujimoto, Michael S. Patrick, Dai Chida, Yoshinori Sato, Yoshinao Azuma, Hiroki Aoki, Takaya Abe, Harumi Suzuki, Mutsunori Shirai, JOURNAL OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, 182(2), 957 - 962, Jan. 2009 , Refereed
    Summary:RhoH is an atypical small G protein with defective GTPase activity that is specifically expressed in hematopoietic lineage cells. RhoH has been implicated in regulation of several physiological processes including hematopoiesis, integrin activation, and T cell differentiation and activation. In the present study, we investigated the role of RhoH in mast cells by generating RhoH knockout mice. Despite observing normal development of mast cells in vivo, passive systemic anaphylaxis and histamine release were impaired in these mice. We also observed defective degranulation and cytokine production upon Fc epsilon RI ligation in RhoH-deficient bone marrow-derived mast cells. Furthermore, Fc epsilon RI-dependent activation of Syk and phosphorylation of its downstream targets, including LAT, SLP76, PIC gamma 1, and PLC gamma 2 were impaired, however phosphorylation of the gamma-subunit of Fc epsilon RI remained intact. We also found RhoH-Syk association that was greatly enhanced by active Fyn. Our results indicate that RhoH regulates Fc epsilon RI signaling in mast cells by facilitating Syk activation, possibly as an adaptor molecule for Syk. The Journal of Immunology, 2009, 182: 957-962.
  • Genome-wide analysis of Chlamydophila pneumoniae gene expression at the late stage of infection, Koshiro Miura, Hidehiro Toh, Hideki Hirakawa, Manabu Sugii, Masayuki Murata, Kenta Nakai, Kosuke Tashiro, Satoru Kuhara, Yoshinao Azuma, Mutsunori Shirai, DNA RESEARCH, DNA RESEARCH, 15(2), 83 - 91, Apr. 2008 , Refereed
    Summary:Chlamydophila pneumoniae, an obligate intracellullar eubacterium, changes its form from a vegetative reticulate body into an infectious elementary body during the late stage of its infection cycle. Comprehension of the molecular events in the morphological change is important to understand the switching mechanism between acute and chronic infection, which is deemed to relate to the pathogenesis of atherosclerosis. Herein, we have attempted to screen genes expressed in the late stage with a genome-wide DNA microarray, resulting in nomination of 17 genes as the late-stage genes. Fourteen of the 17 genes and six other genes predicted as late-stage genes were confirmed to be up-regulated in the late stage with a quantitative reverse transcriptase-pollymerase chain reaction. These 20 late-stage genes were classified into two groups by clustering analysis: 'drastically induced' and 'moderately induced' genes. Out of eight drastically induced genes, four contain sigma(28) promoter-like sequences and the other four contain an upstream common sequence. It suggests that besides sigma(28) there are certain up-regulatory mechanisms at the late stage, which may be involved in the chlamydial morphological change and thus pathogenesis.
  • Molecular characterisation of 12 Chlamydophila felis polymorphic membrane protein genes, Ross Harley, Alan Herring, Kathy Egan, Pam Howard, Tim Gruffydd-Jones, Yoshinao Azuma, Mutsumori Shirai, Chris Helps, VETERINARY MICROBIOLOGY, VETERINARY MICROBIOLOGY, 124(3-4), 230 - 238, Oct. 2007 , Refereed
    Summary:A group of genes thought to encode members of the unique chlamydial polymorphic membrane protein (pmp) family were recently described in the Chlamydophila felis genome. This study aimed to commence characterisation of a subset of 12 of these putative pmp genes by developing and using gene-specific real-time (Q)PCR assays to confirm their presence in a wide range of C. felis field isolates and laboratory strains, and to look for pmp mRNA expression during in vitro infection. Sequencing of 525698 base pair regions of pmp genes 7,9-11, 13-20 for two laboratory strains of C.felis and alignment with the published Fe/C-56 sequence found only a single nucleotide polymorphism present in pmp9. Following the development of gene-specific (Q)PCR assays, analysis of genomic DNA extracted from 40 C. felis field isolates and 4 laboratory strains found that all 12 pmp genes were represented in all cases. Reverse transcription (RT)-QPCR analysis of RNA extracted from cell cultures at 24 and 48 h post inoculation with I of 5 different strains of C. felis detected transcripts for all 12 pmp genes at both time points. Analysis of the relative levels of pmp gene transcription suggested that down-regulation of the expression of multiple C. felis pmp genes occurs between 24 and 48 h post inoculation. This study provides the first evidence that 12 of the putative pmp C. felis genes are transcribed during in vitro infection, and shows that these genes are present in a large range of C felis field isolates and multiple passage laboratory-grown strains. (c) 2007 Elsevier B.V. All rights reserved.
  • Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4(+) CD8(+) immature thymocyte line, Hiroyo Oda, Harumi Suzuki, Kouhei Sakai, Seiji Kitahara, Michael S. Patrick, Yoshinao Azuma, Kazuro Sugi, Toshio Kitamura, Jonathan Kaye, Mutsunori Shirai, JOURNAL OF LEUKOCYTE BIOLOGY, JOURNAL OF LEUKOCYTE BIOLOGY, 81(2), 500 - 508, Feb. 2007 , Refereed
    Summary:Rac1, one of the Rho family small guanosine triphosphatases, has been shown to work as a "molecular switch" in various signal transduction pathways. To assess the function of Rac1 in the differentiation process of CD4 single-positive (CD4-SP) T cells from CD4CD8 double-positive (DP) cells, we used a DP cell line DPK, which can differentiate into CD4-SP cells upon TCR stimulation in vitro. DPK expressing dominant-negative (dn)Rac1 underwent massive apoptosis upon TCR stimulation and resulted in defective differentiation of CD4-SP cells. Conversely, overexpression of dnRac2 did not affect differentiation. TCR-dependent actin polymerization was inhibited, whereas early ERK activation was unaltered in duRac2-expressing DPK. We found that TCR-dependent induction of Bcl-2 was suppressed greatly in dnRac1-expressing DPK, and this suppression was independent of actin rearrangement. Furthermore, introduction of exogenous Bcl-2 inhibited TCR-dependent induction of apoptosis and restored CD4-SP generation in dnRac1-expressing DPK without restoring TCR-induced actin polymerization. Collectively, these data indicate that Rac1 is critical in differentiation of CD4-SP from the DP cell line by preventing TCR-induced apoptosis via Bcl-2 up-regulation.
  • Chlamydial SET domain protein functions as a histone met hyltransferase, Masayuki Murata, Yoshinao Azuma, Koshiro Miura, Mohd. Akhlakur Rahman, Minenosuke Matsutani, Masahiro Aoyama, Harumi Suzuki, Kazuro Sugi, Mutsunori Shirai, MICROBIOLOGY-SGM, MICROBIOLOGY-SGM, 153, 585 - 592, Feb. 2007 , Refereed
    Summary:SET domain genes have been identified in numbers of bacterial genomes based on similarity to SET domains of eukaryotic histone methyltransferases. Herein, a Chlamydophila pneumoniae SET domain gene was clarified to be coincidently expressed with hctA and hctB genes encoding chlamydial histone H1-like proteins, Hc1 and Hc2, respectively. The SET domain protein (cpnSET) is localized in chlamydial cells and interacts with Hc1 and Hc2 through the C-terminal SET domain. As expected from conservation of catalytic sites in cpnSET, it functions as a protein methyltransferase to murine histone H3 and Hc1. However, little is known about protein methylation in the molecular pathogenesis of chlamydial infection. cpnSET may play an important role in chlamydial cell maturation due to modification of chlamydial histone H1-like proteins.
  • Phosphoinositide 3-kinase in nitric oxide synthesis in macrophage - Critical dimerization of inducible nitric-oxide synthase, K Sakai, H Suzuki, H Oda, T Akaike, Y Azuma, T Murakami, K Sugi, T Ito, H Ichinose, S Koyasu, M Shirai, JOURNAL OF BIOLOGICAL CHEMISTRY, JOURNAL OF BIOLOGICAL CHEMISTRY, 281(26), 17736 - 17742, Jun. 2006 , Refereed
    Summary:Phosphoinositide 3-kinase (PI3K) has important functions in various biological systems, including immune response. Although the role of PI3K in signaling by antigen-specific receptors of the adaptive immune system has been extensively studied, less is known about the function of PI3K in innate immunity. In the present study, we demonstrate that macrophages deficient for PI3K (p85 alpha regulatory subunit) are impaired in nitric oxide ( NO) production upon lipopolysaccharide and interferon-gamma stimulation and thus vulnerable for intracellular bacterial infection such as Chlamydophila pneumoniae. Although expression of inducible nitric-oxide synthase (iNOS) is induced normally in PI3K-deficient macrophages, dimer formation of iNOS protein is significantly impaired. The amount of intracellular tetrahydrobiopterin, a critical stabilizing cofactor for iNOS dimerization, is decreased in the absence of PI3K. In addition, induction of GTP cyclohydrolase 1, a rate-limiting enzyme for biosynthesis of tetrahydrobiopterin, is greatly reduced. Our current results demonstrate a critical role of class IA type PI3K in the bactericidal activity of macrophages by regulating their NO production through GTP cyclohydrolase 1 induction.
  • Genome sequence of the cat pathogen, Chlamydophila felis, Yoshinao Azuma, Hideki Hirakawa, Atsushi Yamashita, Yan Cai, Mohd Akhlakur Rahman, Harumi Suzuki, Shigeki Mitaku, Hidehiro Toh, Susumu Goto, Tomoyuki Murakami, Kazuro Sugi, Hideo Hayashi, Hideto Fukushi, Masahira Hattori, Satoru Kuhara, Mutsunori Shirai, DNA RESEARCH, DNA RESEARCH, 13(1), 15 - 23, Feb. 2006 , Refereed
    Summary:Chlamydophila felis (Chlamydia psittaci feline pneumonitis agent) is a worldwide spread pathogen for pneumonia and conjunctivitis in cats. Herein, we determined the entire genomic DNA sequence of the Japanese C. felis strain Fe/C-56 to understand the mechanism of diseases caused by this pathogen. The C. felis genome is composed of a circular 1 166 239 bp chromosome encoding 1005 protein-coding genes and a 7552 bp circular plasmid. Comparison of C. felis gene contents with other Chlamydia species shows that 795 genes are common in the family Chlamydiaceae species and 47 genes are specific to C. felis. Phylogenetic analysis of the common genes reveals that most of the orthologue sets exhibit a similar divergent pattern but 14 C. felis genes accumulate more mutations, implicating that these genes may be involved in the evolutional adaptation to the C. felis-specific niche. Gene distribution and orthologue analyses reveal that two distinctive regions, i.e. the plasticity zone and frequently gene-translocated regions (FGRs), may play important but different roles for chlamydial genome evolution. The genomic DNA sequence of C. felis provides information for comprehension of diseases and elucidation of the chlamydial evolution.
  • Serotonin and melatonin, neurohormones for homeostasis, as novel inhibitors of infections by the intracellular parasite chlamydia, MA Rahman, Y Azuma, H Fukunaga, T Murakami, K Sugi, H Fukushi, K Miura, H Suzuki, M Shirai, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 56(5), 861 - 868, Nov. 2005 , Refereed
    Summary:Objectives: Chlamydiae are obligate intracellular bacteria, causing a variety of diseases, i.e. pneumonia, sexually transmitted disease, conjunctivitis and zoonosis. Tryptophan depletion by interferon-gamma (IFN-gamma) is the most important host defence system against chlamydial infection. Thus chlamydial tryptophan metabolism is thought to play key roles for IFN-gamma resistance, persistent infection and host/tissue tropisms. We tested tryptophan derivatives for activity against chlamydia-infected cells. Methods: Rates of chlamydial infection and sizes of the inclusions were evaluated by in vitro infection using three Chlamydiaceae species, Chlamydia trachomatis, Chlamydophila pneumoniae and Chlamydophila felis, which show significant divergence of tryptophan synthesis genes and different susceptibilities to IFN-gamma. Results: Melatonin and serotonin, which are recognized as neural hormones for maintenance of organism homeostasis, reduced chlamydial infection but not other bacterial growth tested here. Unlike IFN-gamma, melatonin limited infection of all three chlamydiae and the effects were not recovered by tryptophan supplementation. Melatonin treatment only of host cells could diminish infection and the infection reduction was neutralized by a pertussis toxin, an inhibitor of G proteins. Ligands of melatonin and serotonin receptors also hampered infection. Conclusions: Inhibition mechanisms of chlamydial infection by melatonin and serotonin appear to be different from those of IFN-gamma and involve specific G-protein-coupled receptors. Melatonin is deemed to inhibit early progression of the chlamydial development cycle, such as establishment of intracellular infection and/or conversion from elementary body to reticulate body. Utilization of melatonin, serotonin or their derivatives may be advantageous for harmless prevention of chlamydial infection.
  • Identification of genes in the complete genomic DNA sequence of a hyper-thermophilic archaeon, Pyrococcus sp. OT3, Y Ohfuku, H Koike, Y Azuma, T Kawashima, N Kudo, N Amano, J Kakinuma, JM Suckow, M Suzuki, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 74(5), 90 - 95, May 1998 , Refereed
    Summary:By using the algorithm for identifying transcription units-i.e. independently regulated ORFs and operons, and pseudo-genes, that was described in our earlier paper (Suckow et al., FEES Letters, in press), the 1,738,505 base sequence that covers the whole genome of Pyrococcus sp. OT3 has been analyzed. ORFs of the number, 1,819, and 51 RNA genes have been identified. For 42% of the identified ORFs significant homology has been found to the known protein genes of known function, and thus these were identified as protein genes, while for 31% homology has been found to the known ORFs of unknown function. For the rest 27% no significant homology has been found.
  • Phosphorylation of Srp1p, the yeast nuclear localization signal receptor, in vitro and in vivo, Y Azuma, K Takio, MM Tabb, L Vu, M Nomura, BIOCHIMIE, BIOCHIMIE, 79(5), 247 - 259, May 1997 , Refereed
    Summary:Srp1p, the protein encoded by SRP1 of the yeast Saccharomyces cerevisiae, is a yeast nuclear localization signal (NLS) receptor protein. We have previously reported isolation of a protein kinase from yeast extracts that phosphorylates Srp1p complexed with NLS peptides/proteins. From partial amino acid sequences of the four subunits of the purified kinase, we have now identified this protein kinase to be identical to yeast casein kinase II (CKII). It was previously thought that autophosphorylation of the 36 kDa subunit of the yeast enzyme was stimulated by the substrate, GST-Srp1p. However, with the use of a more refined system, no stimulation of autophosphorylation of the 36 kDa subunit of yeast CKII was observed. Biochemical and mutational analyses localized the in vitro phosphorylation site of Srp1p by CKII to serine 67. It was shown that, in the absence of NLS peptides/proteins, phosphorylation of the intact Srp1p protein is very weak, but deletion of the C-terminal end causes great stimulation of phosphorylation without NLS peptides/proteins. Thus, the CKII phosphorylation site is apparently masked in the intact protein structure by the presence of a C-terminal region, probably between amino acids 403 and 516. Binding of NLS peptides/proteins most likely causes a change in protein conformation, exposing the CKII phosphorylation site. Mutational alterations of serine 67, the CKII phosphorylation site, to valine (S67V) and aspartic acid (S67D) were not found to cause any significant deleterious effects on cell growth. Analysis of in vivo phosphorylation showed that at least 30% of the wild type Srp1p molecules are phosphorylated in growing cells, and that the phosphorylation is mostly at the serine 67 CKII site. The ability of Srp1p purified from E coli and treated with calf intestinal phosphatase to bind a SV40 T-antigen NLS peptide was compared with that of Srp1p which was almost fully phosphorylated by CKII. No significant difference was observed. It appears that NLS binding does not require any phosphorylation of Srp1p, either by CKII or by some other protein kinase.
  • ISOLATION OF THERMOLABILE MUTANT RNA-POLYMERASE-II FROM FISSION YEAST SCHIZOSACCHAROMYCES-POMBE WITH MUTATIONS IN THE SUBUNIT-3 GENE, Y AZUMA, K YASUI, M YAMAGISHI, A ISHIHAMA, JOURNAL OF BIOCHEMISTRY, JOURNAL OF BIOCHEMISTRY, 118(1), 216 - 220, Jul. 1995 , Refereed
    Summary:Subunit alpha of prokaryotic RNA polymerases plays key roles in protein-protein contacts for both subunit assembly and transcription activation, To gain an insight into the roles of subunit 3, the eukaryotic homologue of alpha, temperature-sensitive mutants of the fission yeast, Schizosaccharomyces pombe, have been isolated after transformation of the mutagenized rpb3 gene encoding mutant subunit 3 of RNA polymerase II, A total of 68 ts mutants were classified into two groups: mutants comprising one group ceased growing immediately after a temperature up-shift, while mutants comprising the other group exhibited delayed growth arrest at high temperatures, RNA polymerase II partially purified from Ts54, one of the group 2 mutants, was thermolabile in vitro, as measured by a non-specific transcription assay, This mutant carries double mutations in domain A of subunit 3, and thus can be used as a reference mutant of RNA polymerase II.
  • ISOLATION OF A YEAST PROTEIN-KINASE THAT IS ACTIVATED BY THE PROTEIN ENCODED BY SRP1 (SRP1P) AND PHOSPHORYLATES SRP1P COMPLEXED WITH NUCLEAR-LOCALIZATION SIGNAL PEPTIDES, Y AZUMA, MM TABB, L VU, M NOMURA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 92(11), 5159 - 5163, May 1995 , Refereed
    Summary:Srp1p, the protein encoded by SRP1 of Saccharomyces cerevisiae, is a nuclear-pore-associated protein. Its Xenopus homolog, importin, was recently shown to be an essential component required for nuclear localization signal (NLS)-dependent binding of karyophilic proteins to the nuclear envelope [Gorlich, D., Prehn, S., Laskey, R. A. & Hartman, E. (1994) Cell 79, 767-778]. We have discovered a protein kinase whose activity is stimulated by Srp1p (Srp1p fused to glutathione S-transferase and expressed in Escherichia coli) and is detected by phosphorylation of Srp1p and of a 36-kDa protein, a component of the protein kinase complex. The enzyme, called Srp1p kinase, is a protein-serine kinase and was found in extracts in two related complexes of approximate to 180 kDa and 220 kDa. The second complex, when purified, contained four protein components including the 36-kDa protein. We observed that, upon purification of the kinase, phosphorylation of Srp1p became very weak, while activation of phosphorylation of the 36-kDa protein by Srp1p remained unaltered. Significantly, NLS peptides and the nuclear proteins we have tested greatly stimulated phosphorylation of Srp1p, suggesting that Srp1p, complexed with karyophilic proteins carrying an NLS, is the in vivo substrate of this protein kinase.
  • MOLECULAR DISSECTION OF INFLUENZA-VIRUS NUCLEOPROTEIN - DELETION MAPPING OF THE RNA-BINDING DOMAIN, M KOBAYASHI, T TOYODA, DM ADYSHEV, Y AZUMA, A ISHIHAMA, JOURNAL OF VIROLOGY, JOURNAL OF VIROLOGY, 68(12), 8433 - 8436, Dec. 1994 , Refereed
    Summary:Influenza virus nucleoprotein (NP) is associated with the genome RNA, forming ribonucleoprotein cores. To identify the amino acid sequence involved in RNA binding, we performed Northwestern blot analysis with a set of N- and C-terminal deletion mutants of NP produced in Escherichia coli. The RNA binding region has been mapped between amino acid residues 91 and 188, a stretch of residues that contains a sequence that is not only highly conserved among NPs from A-, B-, and C-type influenza viruses but also similar to the RNA binding domain of a plant virus movement protein.
  • SUBUNITS OF THE SCHIZOSACCHAROMYCES-POMBE RNA POLYMERASE-II - ENZYME-PURIFICATION AND STRUCTURE OF THE SUBUNIT 3 GENE, Y AZUMA, M YAMAGISHI, A ISHIHAMA, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 21(16), 3749 - 3754, Aug. 1993 , Refereed
    Summary:To improve our understanding of the structure and function of eukaryotic RNA polymerase II, we purified the enzyme from the fission yeast Schizosaccharomyces pombe. The highly purified RNA polymerase II contained more than eleven polypeptides. The sizes of the largest, the second-, and the third-largest polypeptides as measured by SDS-polyacrylamide gel electrophoresis were about 210, 150, and 40 kilodaltons (kDa), respectively, and are similar to those of RPB1, 2, and 3 subunits of Saccharomyces cerevisiae RNA polymerase II. Using the degenerated primers designed after amino acid micro-sequencing of the 40 kDa third-largest polypeptide (subunit 3), we cloned the subunit 3 gene (rpb3) and determined its DNA sequence. Taken together with the sequence of parts of PCR-amplified cDNA, the predicted coding sequence of rpb3, interrupted by two introns, was found to encode a polypeptide of 297 amino acid residues in length with a molecular weight of 34 kDa. The S.pombe subunit 3 contains four structural domains conserved for the alpha-subunit family of RNA polymerase from both eukaryotes and prokaryotes. A putative leucine zipper motif was found to exist in the C-terminal proximal conserved region (domain D). Possible functions of the conserved domains are discussed.
  • EXPRESSION OF HUMAN P-GLYCOPROTEIN IN YEAST-CELLS - EFFECTS OF MEMBRANE COMPONENT STEROLS ON THE ACTIVITY OF P-GLYCOPROTEIN, T SAEKI, AM SHIMABUKU, Y AZUMA, Y SHIBANO, T KOMANO, K UEDA, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 55(7), 1859 - 1865, Jul. 1991 , Refereed
    Summary:A human MDR1 cDNA was introduced into yeast cells. Immunoblot analysis and indirect immunostaining showed that some of the P-glycoprotein produced was situated in its native orientation in the yeast plasma membrane. Drug-binding activities of the recombinant P-glycoproteins were markedly decreased compared to that of the authentic P-glycoprotein. To identify the bases of decreased binding we studied the effects of membrane component sterols on the azidopine binding and found that ergosterol, which is the main sterol in the yeast membrane, and calciferol, which is produced from ergosterol by UV irradiation, inhibited azidopine binding. These sterols in yeast membrane probably inhibit the function of human P-glycoprotein as a multidrug transporter in yeast cells, because expression of P-glycoprotein in yeast cells did not confer resistance to doxorubicin.
  • CLONING AND SEQUENCE DETERMINATION OF THE SCHIZOSACCHAROMYCES-POMBE RPB1 GENE ENCODING THE LARGEST SUBUNIT OF RNA POLYMERASE-II, Y AZUMA, M YAMAGISHI, R UESHIMA, A ISHIHAMA, NUCLEIC ACIDS RESEARCH, NUCLEIC ACIDS RESEARCH, 19(3), 461 - 468, Feb. 1991 , Refereed
    Summary:The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.

Books etc

  • Genomics of Chlamydia, Joint author,   2003
  • Subunit Function of RNA Polymerase II from the Fission Yeast Schizosaccharomyces pombe: Subunit 2, 3 and 5, MIYAO T, YASUI K, KAWAGISHI M, AZUMA Y, YAMAGISHI M, ISHIHAMA A, Joint author,   1995

Conference Activities & Talks

  • Analysis of a Novel Plasmid of Halomonas sp. and Their Utilization, Ayaka Tsuji, Taku Nishimura, Yoshinao Azuma,   2019 07 08
  • Establishment of a callus line for stable proliferation and rooting regulation using Chinese cabbage.,   2018 03 17
  • Isolation of Halomonas Producing Pyruvate and 3-Hydroxybutyrate,   2018 03 16
  • 日和見病原菌Asaia bogorensisの環境適応遺伝子群, 河合 幹彦, 東裏 典枝, 早崎 君江, 平川 英樹, 武部 聡, 松下 一信, 東 慶直, 第9回日本ゲノム微生物学会年会,   2015 03 06 , ゲノム微生物学会
  • Genome and RNA-seq analyses reveal adaptive responses of opportunistic pathogen Asaia bogorensis, 河合 幹彦, 東 慶直, 2015年日本細菌学会,   2015 03 , 日本細菌学会
  • 酢酸菌の耐熱性育種株およびOmics解析, Yoshinao Azuma, 日本農芸化学会2015年度大会シンポジウム,   2015 03 , 招待有り, 日本農芸化学会
  • Thermo-tolerant modification of acetic acid bacteria by adaptation, genetic recombination and cell fusion, Yoshinao Azuma, International Symposium of Research Center for Thermotolerant Microbial Resources Memorial for moving form Faculty center to University Center “New Era for Microbiology facing to Global Climate Change,   2015 03 , 招待有り, 中高温微生物センター
  • 新規ストレス耐性化(耐熱化)微生物の作出方法, Yoshinao Azuma,   2015 01 13 , 新技術説明会
  • 酢酸菌Acetobacter pasteurianus NBRC3283由来の 耐熱性育種株と細胞融合株を用いた、合計1tの食酢発酵試験, Yoshinao Azuma, 山口大学中高温微生物研究センター発酵部門セミナー,   2014 12 22 , 招待有り, 山口大学中高温微生物研究センター発酵部門セミナー
  • 細胞融合および育種による耐熱性酢酸菌を用いた食酢醸造試験, 岡本 成平, 山本 美佳, 塩村 紗奈, 熊田 彩花, 西村 芽伊, 状家 佳苗, 武部 聡, 田村 章弘, 東 慶直, 酢酸菌研究会・乳酸菌学会合同研究集会,   2014 12 05 , 酢酸菌研究会
  • Genome and RNA-seq analyses of Asaia bogorensis reveal adaptive responses as an opportunistic pathogen, 河合 幹彦, 東裏 典枝, 早崎 君江, 平川 英樹, 武部 聡, 松下 一信, 東 慶直, 酢酸菌研究会・乳酸菌学会合同研究集会,   2014 12 05 , 酢酸菌研究会
  • 酢酸菌のセルロース合成能に関するゲノムワイド遺伝子発現解析, 新川 悠一, 松下 一信, 東 慶直, 酢酸菌研究会・乳酸菌学会合同研究集会,   2014 12 05 , 酢酸菌研究会
  • Unique stress-responsible genes of alphaproteobacterial Asaia bogorensis revealed by whole genome sequencing and RNA-seq, Mikihiko Kawai, Norie Higashiura, Kimie Hayasaki, Naruhei Okamoto, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 分子生物学会,   2014 10 25 , 分子生物学会
  • 抗感染性カテーテルを目指したフッ素ドープアパタイトナノ粒子の開発, 古薗 勉, 東 慶直, 澤 芳樹, H26日本セラミックス協会 第27回秋季シンポジウム,   2014 09 09 , 日本セラミックス協会
  • 銀ドープアパタイトナノ粒子の合成と融着防止剤の影響, 古薗 勉, マズンデル茂田春Mazumder Motaharul, 東 慶直, 澤 芳樹, H26日本セラミックス協会 第27回秋季シンポジウム,   2014 09 09 , 日本セラミックス協会
  • Gene organization of large plasmids of novel mosquitocidal Bacillus thuringiensis TK-E6, Mayu Noda, Naruhei Okamoto, Kimie Hayasaki, Yoshinao Azuma, So Takebe, 2014 International Congress on Invertebrate Pathology and Microbial Control & 47th Annual Meeting of the Society for Invertebrate Pathology,   2014 08
  • Experimental and bioinformatic analyses of stress tolerant acetic acid bacteria, Yoshinao Azuma, ALCA/JST presents Workshop on Advanced Low-Carbon Biotechnology,   2014 07 03 , 招待有り, Thermo-tolerant
  • 遺伝子導入による耐熱化, Yoshinao Azuma, ALCAサイトビジット,   2014 06 01 , ALCAサイトビジット
  • 肺炎クラミジアの封入体膜タンパク質 IncA2 による Apaf-1 非依 存性 caspase-9 の活性化, Yoshinao Azuma, 2014年日本細菌学会シンポジウム 「細菌による持続感染の成立と発症の分水嶺」,   2014 03 28 , 招待有り, 日本細菌学会
  • 「Acetobacter pasteurianus耐熱化育種株42℃の遺伝子発現解析」, Yoshinao Azuma, 山口大学中高温微生物研究センター発酵部門セミナー,   2014 03 07 , 招待有り, 山口大学中高温微生物研究センター発酵部門セミナー
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis, Hiromi Hadano, Naruhei Okamoto, Norie Higashiura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 第36回日本分子生物学会年会,   2013 12 05 , 分子生物学会
  • 新規静菌性デバイス開発を目指した銀ナノ粒子の合成と材料特性, 古薗 勉, 岩本 多加志, 東 慶直, 岡田 正弘, 澤 芳樹, 日本バイオマテリアル学会,   2013 11 25
  • Draft Genomic DNA Sequence of the Facultatively Methylotrophic Bacterium Acidomonas methanolica type strain MB58, Norie Higashiura, Hiromi Hadano, Hideki Hirakawa, Minenosuke Matsutani, Ryoji Mitsui, Sou Takebe, Kazunobu Matsushita, Yoshinao Azuma, 酢酸菌研究会 第5回研究集会,   2013 10 , 酢酸菌研究会
  • 酢酸菌のストレス耐性化育種とゲノム解析, Yoshinao Azuma, ALCAセミナー,   2013 07 04 , 招待有り, ALCAセミナー
  • Genome Analyses for High Glucose Tolerance of Acetic Acid Bacteria, Hiromi Hadano, Norie Higashiura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 113rd General Meeting: American society for Microbiology,   2013 05 , ASM
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Norie Higashiura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, ○Yoshinao AZUMA, 2013年度日本農芸化学会大会,   2013 03 27 , 日本農芸化学会
  • 酢酸菌のストレス応答に関するゲノム解析, Yoshinao Azuma, 第7回日本ゲノム微生物学会年会,   2013 03 08 , ゲノム微生物学会
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 第35回日本分子生物学会年会,   2012 12 14 , 分子生物学会
  • 酢酸菌の耐熱化,あれやこれや, 波多野 裕美, 山崎 万愛, 高見 晶子, 東裏 典枝, 武部 聡, 松下 一信, 東 慶直, 山口大学 第4回 発酵微生物部門セミナー,   2012 12 08 , 招待有り, 山口大学 第4回 発酵微生物部門セミナー
  • Genome analyses for hyper glucose tolerance of an acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis, Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 第4回酢酸菌研究会,   2012 11 10 , 酢酸菌研究会
  • 酢酸菌が有する耐ストレス性の利用に向けた酢酸菌科細菌のパン・ゲノム解析, Yoshinao Azuma, 第23回大学間学術交流会,   2012 10 13 , 研究交流会
  • Genome analysis for hyper glucose tolerance of Tanticharoenia sakaeratensis and Asaia bogorensis., 波多野 裕美, 東 慶直, 第23回大学間学術交流会,   2012 10 13 , 研究交流会
  • 近紫外線による院内感染の発生箇所の浄化, Yoshinao Azuma, 第1回ねごろバイオマテリアル研究会,   2012 08 15 , 第1回ねごろバイオマテリアル研究会
  • 耐熱性酢酸菌の比較ゲノム解析と発現解析, 東 慶直, 波多野 裕美, 高見 晶子, 長井 秀樹, 山崎 万愛, 木村 萌美, 松谷 峰之介, 松下 一信, ALCAバイオテクノロジー分科会,   2012 08 08 , ALCA発表会山口大学
  • 次世代シークエンサーを用いた酢酸菌の遺伝子発現解析, 東 慶直, 波多野 裕美, 高見 晶子, 長井 秀樹, 山崎 万愛, 木村 萌美, ALCA発表会山口大学,   2012 06 15 , ALCA発表会山口大学
  • Genome analysis for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 3rd International Meeting of Acetic Acid Bacteria,   2012 04 12 , 国際酢酸菌学会
  • 酢酸菌Tanticharoenia sakaeratensisの有する高濃度グルコース耐性に対するゲノム解析, 波多野 裕美, 平川 英樹, 松下 一信, 武部 聡, 東 慶直, 日本農芸化学会2012年度大会,   2012 03 24 , 日本農芸化学会
  • degenerate PCRを用いたBacillus thuringiensis由来の殺虫タンパク質遺伝子の検出と単離, 森村 夏花, 波多野 裕美, 久保 友梨佳, 小宮 浩史, 唐谷 ゆふ, 東 慶直, 武部 聡, 日本農芸化学会2012年度大会,   2012 03 23 , 日本農芸化学会
  • 微生物のゲノム解析ー病原性菌から有用細菌までー, Yoshinao Azuma, 第9回アグリバイオ・セミナー,   2012 03 12 , 招待有り, アグリバイオ・セミナー
  • 酢酸菌18菌種のゲノム解析と遺伝子発現解析, Yoshinao Azuma, 第1回ALCA発表会,   2012 02 10 , ALCA
  • 高温環境(および高ストレス環境)下における酢酸菌のロバスト化, Yoshinao Azuma, ALCA発表会山口大学,   2012 02 10 , ALCA発表会山口大学
  • Genome and Proteome analyses for hyper glucose tolerance of an acetic acid bacterium, Tanticharoenia sakaeratensis., 波多野 裕美, 武部 聡, 東 慶直, MBSJ 2011,   2011 12 14 , 分子生物学会
  • 産業有用微生物のストレス耐性化育種とそのゲノム情報解析, Yoshinao Azuma, わかやまテクノビジネスフェア,   2011 12 07 , 招待有り, わかやまテクノ
  • Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis, Yuta Sugimori, Yu Karatani, Hiromi Hadano, Yoshinao Azuma, So Takebe, The IUMS 2011 Sapporo Congress,   2011 09 07 , 国際微生物学会
  • Phenome analyses of the thermo-tolerant strain bred from Acetobacter pasteurianus, Yoshinao Azuma(BOST Kinki Uni, Naoko Furuya(Kyowa Hakko, The IUMS 2011 Sapporo Congress,   2011 09 06 , 国際微生物学会
  • Genome and proteome analyses for hyper glucose tolerance of an acetic acid bacterium,Tanticharoenia sakaeratensis, Hiromi Hadano, Yuta Sugimori, So Takebe, Yoshinao Azuma, The IUMS 2011 Sapporo Congress,   2011 09 06 , 国際微生物学会
  • ANALYSIS OF THERMAL ADAPTATION MECHANISM OF ACETOBACTER PASTEURIANUS SKU1108 USING NEXT GENERATION GENOME SEQUENCER, Minenosuke Matsutani, Natsaran Saichana, Mitsuteru Nishikura, Toshiharu Yakushi, Yoshinao Azuma, Kazunobu Matsushita, The IUMS 2011 Sapporo Congress,   2011 09 06 , 国際微生物学会
  • IDENTIFICATION OF GENES THAT CONTRIBUTE TO THE TEMPERATURE-RESISTANCE IN ACETIC ACID BACTERIUM BY WHOLE-GENOME ANALYSIS, Hidetaka Ogino, Yoshinao Azuma, Minenosuke Matsutani, Mutsunori Shirai, The IUMS 2011 Sapporo Congress,   2011 09 06 , 国際微生物学会
  • Tanticharoenia sakaeratensisの高濃度グルコース耐性の解析, 波多野 裕美, 森村 夏花, 東 慶直, 日本農芸化学会,   2011 03 , 日本農芸化学会
  • Acetobacter pasteurianus高温耐性育種株のphenome解析, 森村 夏花, 波多野 裕美, 東 慶直, 日本農芸化学会,   2011 03 , 日本農芸化学会
  • Genome-wide analysis of thermal adaptation mechanism of Acetobacater pasteurianus SKU1108 using mext generation genome sequencer, 松谷 峰之介, 西倉 慎顕, Natharan Saichana, 今田 智子, 秦野 智行, 薬師 寿治, 東 慶直, 白井 睦訓, 松下 一信, BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会),   2010 12 , 分子生物学会
  • ドラフトゲノム解析による耐熱性酢酸菌Acetobacter pasteurianus SKU1108株の耐熱化機構の解析, 松谷 峰之介, 西倉 慎顕, Natharan Saichana, 今田 智子, 秦野 智行, 薬師 寿治, 東 慶直, 白井 睦訓, 松下 一信, 第2回酢酸菌研究会,   2010 11 13 , 酢酸菌研究会
  • Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis, Yu Karatani, Yoshinao Azuma, So Takebe, 43rd Annual meeting of the society for invertebrate pathology,   2010 07 , invertebrate pathology
  • 酢酸菌のゲノム進化と高温発酵, Yoshinao Azuma, 2010日本農芸化学会,   2010 03 30 , 招待有り, 日本農芸化学会
  • Comparative genome analysis of intracellular bacteria, Yoshinao Azuma, the 10th Japan- Korea International Symposium on Microbiology,   2010 03 26 , 招待有り, Japan- Korea
  • 酢酸菌のゲノム易変異性, 東 慶直, 古谷 直子, 細山 哲, 松谷 峰之介, 平川 英樹, 松下 一信, 久原 哲, 藤田 信之, 白井 睦訓, 第4回日本ゲノム微生物学会年会,   2010 03 , ゲノム微生物学会
  • Bacillus thuringiensisがもつ殺虫タンパク質遺伝子のdegenerate PCRによる探索及び構造解析, 唐谷 ゆふ, 東 慶直, 武部 聡, 日本農芸化学会2010年度大会,   2010 03 , 日本農芸化学会
  • セミ・ドライ研究者による次世代シーケンサーの利用, Yoshinao Azuma, 次世代シーケンス解析セミナー,   2010 01 21 , 招待有り, 次世代シーケンス
  • 比較ゲノム解析に基づく酢酸菌のゲノム易変異性, 東 慶直, 細山 哲, 松谷 峰之介, 古谷 直子, 堀川 博司, 原田 健史, 平川 英樹, 久原 哲, 松下 一信, 藤田 信之, 白井 睦訓, 第32回日本分子生物学会年会,   2009 12 , 分子生物学会
  • 次世代シークエンサを用いた耐熱性酢酸菌Acetobacter pasteurianus SKU1108株のドラフトゲノム解析, 松谷 峰之介, 西倉 慎顕, 今田 智子, 秦野 智行, 藥師 寿治, 東 慶直, 白井 睦訓, 松下 一信, 第1回酢酸菌研究会,   2009 11 07 , 酢酸菌研究会
  • 酢酸菌Acetobacter pasteurianusの代謝マップを書いてみました, 東 慶直, 古谷 直子, 白井 睦訓, 第1回酢酸菌研究会,   2009 11 07 , 酢酸菌研究会
  • 日本紅班熱の病原菌リケチア・ジャポニカの比較ゲノム解析, 松谷 峰之介, 花岡 希, 東 慶直, 吉岡 里美, 小川 基彦, 岸本 寿男, 白井 睦訓, 第61回 日本細菌学会中国・四国支部会,   2009 10 , 日本細菌学会中国・四国支部会
  • 酢酸菌に観察される形質・ゲノムの易変異性の解析, 東 慶直, 松谷 峰之介, 古谷 直子, 白井 睦訓, 第61回日本細菌学会中国・四国支部会,   2009 10 , 日本細菌学会中国・四国支部会
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業」,   2009 09 01 , 農林水産省生研機構
  • Acetic acid bacteria analyses from genome to evolution and phenotype, Yoshinao Azuma, 2nd Satellite seminar in Asean Core Program,   2009 08 24 , 招待有り, Asean Core Program
  • 酢酸菌のゲノムとフェノタイプ, Yoshinao Azuma, 日本バイオインフォマティクス学会システムバイオロジー研究会,   2009 08 18 , 招待有り, 日本バイオインフォマティクス学会
  • 酢酸菌に観察されるゲノム易変異性の解析, 東 慶直, 松谷 峰之介, 古谷 直子, 白井 睦訓, 第82回日本細菌学会総会,   2009 03 13 , 日本細菌学会
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業」,   2009 02 11 , 農林水産省生研機構
  • 酢酸菌のゲノム易変異性の解析, Yoshinao Azuma, 第31回日本分子生物学会,   2008 12 09 , 分子生物学会
  • 「Acetobacter sp. NBRC 3283株のゲノム解析に係る共同研究」、「Gluconacetobacter xylinus NBRC 3288株のゲノム解析に係る共同研究」, Yoshinao Azuma, 第5回酢酸菌ゲノム解析共同研究報告会,   2008 11 06 , 酢酸菌ゲノム解析共同研究
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業」,   2008 11 05 , 農林水産省生研機構
  • Genome analysis of Actic acic bacteria., Yoshinao Azuma, 2nd International Conference on Acetic Acid Bacteria,   2008 11 , 招待有り, Acetic Acid Bacteria
  • 酢酸菌に観察される形質・ゲノムの易変異性の解析, 東 慶直, 松谷 峰之介, 古谷 直子, 白井 睦訓, 第61 回日本細菌学会中国・四国支部総会,   2008 10 19 , 日本細菌学会中国・四国支部会
  • Microbial genomes to its phenotypes and pathogenicities, Yoshinao Azuma, The 1st Young Scientist Seminar in The Asian Core Program,   2008 10 05 , 招待有り, Asean Core Program
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業」,   2008 08 24 , 農林水産省生研機構
  • 肺炎クラミジアによる宿主アポトーシス制御機構の解析, Yoshinao Azuma, 第17回日本アポトーシス研究会,   2008 08 , アポトーシス研究会
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業,   2008 07 29 , 農林水産省生研機構
  • シンポジウム「ゲノムからみた感染症」クラミジアの感染・病原性の解析, Yoshinao Azuma, 第82回日本感染症学会総会・学術講演会,   2008 04 , 招待有り, 日本感染症学会
  • 酢酸菌3種の比較ゲノム解析から見えてくる, Yoshinao Azuma, 2008年度日本農芸化学会総会,   2008 03 28 , 招待有り, 日本農芸化学会
  • 「Acetobacter sp. NBRC 3283株のゲノム解析に係る共同研究」、「Gluconacetobacter xylinus NBRC 3288株のゲノム解析に係る共同研究」, Yoshinao Azuma, 第4回酢酸菌ゲノム解析共同研究報告会,   2008 03 25 , 酢酸菌ゲノム解析共同研究
  • 耐熱性発酵微生物の比較ゲノム解析, Yoshinao Azuma, 農林水産省生研機構「新技術・新分野創出のための基礎研究推進事業」,   2008 03 04 , 農林水産省生研機構
  • 日本紅斑熱病原因菌リケッチア・ジャポニカのゲノム解析, 松谷 峰之介, 東 慶直, 吉岡 里美, 白井 睦訓, 第4回微生物研究推進体研究集会,   2007 12 20 , 微生物推進体
  • 酢酸菌Acetobacter pasteurianus2株の形質とゲノムの比較解析, 東 慶直, 古谷 直子, 松谷 峰之介, 松下 一信, 白井 睦訓, 第4回微生物研究推進体研究集会,   2007 12 20 , 微生物推進体
  • ゲノム解析による有用遺伝子探索, Yoshinao Azuma, 日本農芸化学会2007年中四国・西日本支部合同大会 シンポジウム「ゲノムからの旅立ち」,   2007 09 13 , 招待有り, 日本農芸化学会
  • Chlamydial infection modulates apoptosis regulation of host cells, Yoshinao Azuma、Mohd, A. Rahman, Mutsunori Shirai, 47th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC),,   2007 09 , Antimicrobial Agents and Chemotherapy
  • クラミジアのクロマチン制御メカニズムの解明, 白井 睦訓, 村田 正之, 東 慶直, 平成19年度中四国乳酸菌研究会 全日空ホテル,   2007 05 25 , 中四国乳酸菌研究会
  • Host Apoptosis Regulation by Chlamydia Infection, Yoshinao Azuma, Mohd A. Rahman, Mutsunori Shirai, American Society for Microbiology the 107th General Meeting,,   2007 05 , American Society for Microbiology
  • 「Acetobacter sp. NBRC 3283株のゲノム解析に係る共同研究」、「Gluconacetobacter xylinus NBRC 3288株のゲノム解析に係る共同研究」, Yoshinao Azuma, 第2回酢酸菌ゲノム解析共同研究報告会,   2007 04 23 , 酢酸菌ゲノム解析共同研究
  • 日本紅斑熱の病原菌Rickettsia japonicaの全ゲノム解析, 松谷 峰之介, 東 慶直, 小川 基彦, 山下 敦士, 岸本 義男, 服部 正平, 白井 睦訓, 第107回山口大学医学会学術講演会,   2007 02 17 , 山口大学医学会
  • Obstructed NO production from Chlamydophila pneumoniae infected Macrophage, Kouhei Sakai, Yoshinao Azuma, Seiji Kitahara, Mutsunori Shirai, 第36回免疫学会学術総会,   2006 12 11 , 免疫学会
  • 山口大学における微生物ゲノム解析の動向, Yoshinao Azuma, 第3回微生物研究推進体集会,   2006 10 18 , 微生物推進体
  • Apoptotic Protease Activating Factor-1(Apaf-1)-independent Caspase-9 activation by Chlamydophila pneumoniae, Mohd. Akhlakur Rahman, Yoshinao Azuma, Mutsunori Shirai, 第59回日本細菌学会中国・四国支部総会,   2006 10 , 日本細菌学会中国・四国支部会
  • 「Acetobacter sp. NBRC 3283株のゲノム解析に係る共同研究」、「Gluconacetobacter xylinus NBRC 3288株のゲノム解析に係る共同研究」, Yoshinao Azuma, 第1回酢酸菌ゲノム解析共同研究報告会 独立行政法人製品評価技術基盤機構,   2006 09 26 , 酢酸菌ゲノム解析共同研究
  • Genome sequence of Chlamydophila felis and its practical application, Yoshinao Azuma, Mohd. Akhlakur Rahman, H. Hirakawa, A. Yamashita, Y. Cai, S. Mitaku, H. Toh, S. Goto, H. Fukushi, M. Hattori, S. Kuhara, Mutsunori Shirai, The 4th Workshop of COST 855 on Pathogenesis and Diagnosis of Animal Chlamydioses,   2006 09 , 招待有り, COST 855
  • 個別固定遺伝子検出法を用いた肺炎診断の試み, 福永 肇, 東 慶直, 白井 睦訓, 第106回山口大学医学会学術講演会,   2006 07 15 , 山口大学医学会
  • Regulation of Host Cell Apoptosis by Chlamydophila pneumonia, Yoshinao Azuma, Mohd. Akhlakur Rahman, Manabu Sugii, Mutsunori Shirai, The 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,,   2006 06 , 分子生物学会
  • クラミジアのゲノム解析を基盤とする宿主との相互作用解析, Yoshinao Azuma, 第3回分子医科学研究会,   2006 03 31 , 分子医科学研究会
  • 細胞内寄生細菌の排除における宿主マクロファージ中のP13キナーゼの役割, 鈴木 春巳, 酒井 幸平, 小田 浩代, 三浦 公志郎, 東 慶直, 白井 睦訓, 第2回山口大学研究推進体「微生物の機能解析および機能開発」研究集会,   2005 12 23 , 微生物推進体
  • 計算科学的手法を用いたたんぱく質相互作用解析, 松谷 峰之介, 村田 正之, 東 慶直, 白井 睦訓, 第2回山口大学研究推進体「微生物の機能解析および機能開発」研究集会,   2005 12 23 , 微生物推進体
  • クラミジア SET タンパク質の機能解析, 村田 正之, 三浦 公志郎, 東 慶直, 白井 睦訓, 第2回山口大学研究推進体「微生物の機能解析および機能開発」研究集会,   2005 12 23 , 微生物推進体
  • The New Function of Serotonin and Melatonin:Specific Biocide for Chlamydiae in Vitro, Mohd. Akhlakur Rahman, Yoshinao Azuma, Mutsunori Shirai, 第2回山口大学研究推進体「微生物の機能解析および機能開発」研究集会,   2005 12 23 , 微生物推進体
  • 肺炎クラミジアの封入体膜タンパク質IncA2と宿主アポトーシスの関係,   2005 12 23
  • LED の生み出す紫色光による殺菌モデル系の構築と殺菌効果, 東 慶直, 木本 光明, 吉岡 里美, 前田 初芽, 白井 睦訓, 小橋 克哉, 田口 常正, 小野 紀之, 知的クラスター創成事業「LED医療部会」研究会講演,   2005 09 05 , 招待有り, 知的クラスター
  • DNAマイクロアレイを用いた肺炎クラミジアの遺伝子発現の解析とそのゲノム上の偏在, 三浦 公志郎, 藤 英博, 東 慶直, 平川 英樹, 田代 康介, 久原 哲, 白井 睦訓, 第7回ワークショップ「微生物ゲノム研究のフロンティア」,   2005 03 , ゲノム微生物学会
  • クラミジアの感染排除における一酸化窒素合成を介したPI3キナーゼの役割, 酒井 幸平, 鈴木 春巳, 小田 浩代, 東 慶直, 三浦 公志郎, 白井 睦訓, 第1回微生物推進体研究集会,   2004 12 21 , 微生物推進体
  • クラミジアSETとヒストン様タンパク質Hc1, Hc2の相互作用解析, 村田 正之, 三浦 公志郎, 東 慶直, 白井 睦訓, 第1回微生物推進体研究集会,   2004 12 21 , 微生物推進体
  • 肺炎クラミジアの真核生物型遺伝子の宿主細胞内機能, 高岡 直央, 東 慶直, 杉井 学, 白井 睦訓, 第1回微生物推進体研究集会,   2004 12 21 , 微生物推進体
  • 偏性細胞内寄生細菌クラミジアのゲノム情報科学的解析, 東 慶直, 白井 睦訓, 第1回微生物推進体研究集会,   2004 12 21 , 微生物推進体
  • 動脈硬化部に高率に感染する肺炎クラミジア, Yoshinao Azuma, 第19回「大学と科学」公開シンポジウム『細菌はなぜ病気を起こすか−ゲノムの特徴,   2004 11 , 招待有り, 未来開拓
  • クラミジアのゲノム解析と利用, Yoshinao Azuma, タンパク質3000講演会,   2004 10 27 , 招待有り, タンパク3000
  • 肺炎クラミジアの遺伝子発現解析とクロマチン構造変化, 三浦 公志郎, 村田 正之, 東 慶直, 鈴木 春巳, 白井 睦訓, 第57回日本細菌学会中国・四国支部総会,   2004 10 , 日本細菌学会中国・四国支部会
  • Comparison of Gene Expression Profile between Distinct Host Cells infected with Distinct Chlamydiae, Koshiro Miura, Yoshinao Azuma, Mutsunori Shirai, TIGR'S 16th International Genome Sequence and Analysis Conference (GSAC XVI),   2004 09 , TIGR
  • The New Function of Serotonin and Melatonin:Specific biocide for Chlamydiae in vitro, Rahman Mohd Akhlakur, 東 慶直, 白井 睦訓, 第102回山口大学医学会学術講演並びに総会,   2004 07 17 , 山口大学医学会
  • クラミジアのゲノム解析と利用, Yoshinao Azuma, タンパク3000プロジェクト「発生・分化とDNAの複製・修復」班会議,   2004 07 , タンパク3000
  • Immune Gene Expression Analysis of Infected Host Cells Using DNA Microarray, Mutsunori Shirai, Yoshinao Azuma, Naoyoshi Takaoka, Harumi Suzuki, Koshiro Miura, 12th International Congress of Immunology,   2004 07 , Immunology
  • ゲノム創薬としてのパトリオットミサイル的抗生剤の開発 〜国民的細菌感染症の原因であるクラミジアの撲滅を目指して〜, Yoshinao Azuma, 山口大学ベンチャービジネス・ラボラトリー発表会,   2004 03 19 , 山口大学VBL
  • クラミジアのゲノム解析から生物起源と病原性, Yoshinao Azuma, 第21回日本クラミジア研究会、第10回リケッチア研究会,   2003 11 , クラミジア研究会
  • Chlamydophila felis菌ワクチン・診断用抗原エピトープの全ゲノム網羅的解析による検出, 白井 睦訓, 東 慶直, ベンチャー・ビジネス・ラボラトリー平成15年度 発表会,   2003 10 30 , 山口大学VBL
  • クラミジア感染時のマクロファージにおけるP13キナーゼの役割, 酒井 幸平, 鈴木 春巳, 東 慶直, 三浦 公志郎, 白井 睦訓, 第100回山口大学医学会学術講演並びに総会,   2003 07 19 , 山口大学医学会
  • クラミジアの感染および増殖におけるPI3キナーゼの機能, 酒井 幸平, 鈴木 春巳, 東 慶直, 三浦 公志郎, 白井 睦訓, 第76回日本細菌学会総会,   2003 04 , 日本細菌学会
  • 肺炎クラミジアの細胞内増殖に関わるincA2遺伝子の解析, Yoshinao Azuma, 第99回山口大学医学会,   2003 02 15 , 山口大学医学会
  • 肺炎クラミジアSetタンパク質の局在と発現解析, 村田 正之, 三浦 公志郎, 東 慶直, 白井 睦訓, 第99回山口大学医学会,   2003 02 15 , 山口大学医学会
  • 1. Chlamydia felis Genome DNA analysis. 2. Host-parasite interaction, Chlamydia pneumoniae. 3.Structure analysis of Chlamydia pneumoniae, 東 慶直, 白井 睦訓, 未来開拓 「病原性微生物のゲノム解析」班会議,   2003 02 , 未来開拓
  • クラミジア、フェリスの全ゲノムDNA配列の決定と解析, 東 慶直, 平川 英樹, 山下 敦士, 籐 英博, 蔡燕, 福士 秀人, 服部 正平, 久原 哲, 白井 睦訓, 微生物ゲノムワークショップ,   2003 , 招待有り, ゲノム微生物学会
  • Genome DNA Sequence of Low Pathogenicity Chlamydia of Humans: Chlamydophila felis Fe.Pn1(Chlamydia psittaci feline pneumonitis agent), Yoshinao Azuma, H. Hirakawa, A. Yamashita, H. Toh, C. Yan, H. Fukushi, M. Hattori, S. Kuhara, M. Shirai, 3rd ASM and TIGR Conference on Microbial Genomes,   2003 01 , American Society for Microbiology
  • 細胞内寄生細菌、肺炎クラミジアの真核生物様遺伝子の機能解析, 東 慶直, 田島 歓子, 木本 光明, 高岡 直央, 藤 英博, 白井 睦訓, 第25回日本分子生物学会年会,   2002 12 , 分子生物学会
  • DNAマイクロアレイとRT-PCRによるChlamydia pneumoniaeの遺伝子発現解析, 三浦 公志郎, 杉井 学, 村田 正之, 東 慶直, 白井 睦訓, 第25回日本分子生物学会年会,   2002 12 , 分子生物学会
  • Chlamydia felis genome DNA sequencing progress Chlamydia pneumoniae post-genome analysis < two-hybrid analysis, 東 慶直, 白井 睦訓, 未来開拓「病原性微生物のゲノム解析」班会議,   2002 03 , 未来開拓
  • クラミジア感染機構の比較ゲノム解析, 東 慶直, 藤 英博, 三浦 公志郎, 白井 睦訓, 先端技術によるゲノム創薬シンポジウム,   2002 03 01 , 招待有り, ゲノム創薬
  • 肺炎クラミジア感染細胞における宿主ー病原体間の遺伝子相互作用の解析, 東 慶直, 青山 雅博, 田島 歓子, 藤 英博, 白井 睦訓, 山口大学医学会学術講演会,   2002 02 16 , 山口大学医学会
  • クラミジアの全ゲノム情報に基づいた配列相同性のない病原性遺伝子ファミリーの解析, 藤 英博, 三浦 公志郎, 杉井 学, 東 慶直, 白井 睦訓, 第24回日本分子生物学会年会,   2001 12 10 , 分子生物学会
  • ネコクラミジアのゲノム解析について, 白井 睦訓, 東 慶直, 未来開拓「病原性微生物のゲノム解析」班会議,   2001 12 04 , 未来開拓
  • ネコクラミジアのゲノム解析について, 白井 睦訓, 東 慶直, 未来開拓「病原性微生物のゲノム解析」班会議,   2001 10 05 , 未来開拓
  • Chlamydia pneumoniae & Chlamydia felis postgenome projects, 白井 睦訓, 東 慶直, 青山 雅博, 藤 英博, 三浦 公志郎, 村田 正之, 未来開拓「病原性微生物のゲノム解析」班会議,   2001 05 11 , 未来開拓
  • Informatical analysis of archaeal genomic DNA sequences., Yoshinao Azuma, Yuko Ohfuku, Hideaki Koike, Naoki Amano, Jun Kakinuma, Masaru Tateno, Joerg M. Suckow, Masashi Suzuki, Third Annual Conference on Microbial Genomes (The Institute for Genomic Research),   1999 01 , American Society for Microbiology
  • Towards understanding of the transcription network of archaebacteria, Yoshinao Azuma, Michiko Ihara, Masahiro Yamagishi, Naoki Amano, Kazuhiko Yamasaki, Tomoko Yamasaki, Keiichirou Hiratsu, Masashi Suzuki, The 6th inernatinal Symposium on Structural Biology and Genomics.,   1999 , 招待有り, Structural Biology and Genomics
  • 古細菌における遺伝子特異的な転写制御機構の研究,   1998 12
  • Model Gene Proposal for archaea Transciption Regulation Study., Yoshinao Azuma, Masahiro Yamagishi, Masashi Suzuki, The Fifth Asian Conference on Transcription(ACT-V),   1998 02 , Asian Conference on Transcription
  • Molecular recognition in the archaeal transcrption regulation., Yoshinao Azuma, Masahiro Yamagishi, Masashi Suzuki, The 5th International Symposium on Bioscience and Human-Technology,   1998 , 招待有り, Structural Biology and Genomics
  • Phosphorylation of the yeast NLS-receptor protein, Srp1p, by casin kinase II., Yoshinao Azuma, Michelle Tabb, Loan Vu, Koji Takio, Masayasu Nomura, Functional Organization of the Nucleus -Keystone symposium,   1997 02 , Keystone symposium
  • Structural analysis of the Schizosaccharomyces pombe rpb1 gene encodingthe largest subunit of RNA polymerase II., Yoshinao Azuma, M.Yamagishi, R.Ueshima, A.Ishihama, The Third Asian Conference on Transcription(ACT-III),   1992 06 , Asian Conference on Transcription

Misc

  • 酢酸菌Komagataeibacter medellinensis NBRC 3288株のセルロース非生産株から生産株への復帰機構の解明, 松谷峰之介, 伊藤光平, 東慶直, 荻野英賢, 白井睦訓, 薬師寿治, 松下一信, 日本ゲノム微生物学会年会要旨集, 9th, 75,   2015 , http://jglobal.jst.go.jp/public/201602209341554091
  • 紅斑熱の媒介者であるRickettsia japonicaの全ゲノムシークエンス解析(Whole Genome Sequencing of the Spotted Fever Disease Agent Rickettsia japonica), 白井 睦訓, 小川 基彦, 花岡 希, 大津山 賢一郎, 岸本 寿男, 安藤 秀二, Matsutani Minenosuke, Takaoka Naohisa, Toh Hidehiro, Yamashita Atsushi, Oshima Kenshiro, Hirakawa Hideki, Kuhara Satoru, Suzuki Harumi, Hattori Masahira, Azuma Yoshinao, 日本化学療法学会雑誌, 62, Suppl.A, 330, 330,   2014 05
  • 酢酸菌Acetobacter pasteurianus IFO3283株の適応育種とその比較ゲノム解析による「耐熱化」遺伝子の解析, 松本奈実, 松谷峰之介, 東慶直, 薬師寿治, 松下一信, 日本分子生物学会年会プログラム・要旨集(Web), 35th, 2P-0015 (WEB ONLY),   2012 , http://jglobal.jst.go.jp/public/201302224239745687
  • 酢酸菌のゲノム易変異性, 東慶直, 古谷直子, 細山哲, 松谷峰之介, 平川英樹, 松下一信, 久原哲, 藤田信之, 白井睦訓, 日本ゲノム微生物学会年会要旨集, 4th, 40,   2010 03 04 , http://jglobal.jst.go.jp/public/201002230723591803
  • 日本紅斑熱病原因菌Rickettsia japonicaのゲノム解析(Complete genome DNA sequence of the east Asian spotted fever disease agent, Rickettsia japonica), 東 慶直, 松谷 峰之介, 花岡 希, 小川 基彦, 岸本 寿男, 安藤 秀二, 白井 睦訓, 日本細菌学雑誌, 65, 1, 100, 100,   2010 02
  • テロの可能性のある病原体等の早期検知・迅速診断法の開発とその評価法の確立に関わる研究 8.リケッチア・コクシエラ・クラミジアの迅速診断法の開発, 安藤秀二, 花岡希, 松谷峰之介, 坂田明子, 岸本寿男, 東慶直, 白井睦訓, テロの可能性のある病原体等の早期検知・迅速診断法の開発とその評価法の確立に関わる研究 平成21年度 総括・分担研究報告書, 65, 73,   2010 , http://jglobal.jst.go.jp/public/201002288869364378
  • 酢酸菌に観察されるゲノム易変異性の解析, 東 慶直, 松谷 峰之介, 古谷 直子, 白井 睦訓, 日本細菌学雑誌, 64, 1, 219, 219,   2009 02
  • 酢酸菌のゲノム易変異性の解析, 東 慶直, 松谷 峰之介, 古谷 直子, 細山 哲, 平川 英樹, 久原 哲, 藤田 信之, 白井 睦訓, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 81回・31回, 1T2, 2,   2008 11
  • 酢酸菌3種の比較ゲノム解析から見えてくる酢酸菌像, 東慶直, 細山哲, 松谷峰之介, 古谷直子, 高本秀司, 薮崎純子, 五斗進, 金久實, 加賀孝之, 久原哲, 松下一信, 藤田信之, 白井睦訓, 日本農芸化学会大会講演要旨集, 2008, SHI17,   2008 03 05 , http://jglobal.jst.go.jp/public/200902221542783590