KINDAI UNIVERSITY


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Tatsuya Moriyama

Profile

FacultyDepartment of Applied Biological Chemistry / Graduate School of Agriculture / KINDAI Research Institute for Agricultural Technology and Innovation
PositionDean Assistant
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/1060-moriyama-tatsuya.html
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Last Updated :2019/10/19

Education and Career

Education

  •  - 1991 , Kyoto University, Graduate School, Division of Agriculture
  •  - 1987 , Kyoto University, Faculty of Agriculture

Academic & Professional Experience

  •   1996 03 ,  - 1997 02 , Visiting scholar, Stanford University

Research Activities

Research Areas

  • Biological science, Cell biology
  • Agricultural chemistry, Food science

Research Interests

  • food allergy, Molecular Food Function, Cell Biology

Published Papers

  • Soyasapogenols reduce cellular triglyceride levels in 3T3-L1 mouse adipocyte cells by accelerating triglyceride lipolysis., Iwamoto K, Kamo S, Takada Y, Ieda A, Yamashita T, Sato T, Zaima N, Moriyama T, Biochemistry and biophysics reports, 16, 44 - 49, Dec. 2018 , Refereed
  • The role of perivascular adipose tissue in the appearance of ectopic adipocytes in the abdominal aortic aneurysmal wall., Kugo H, Moriyama T, Zaima N, Adipocyte, 8(1), 229 - 239, Dec. 2019 , Refereed
  • Pathological Implication of Adipocytes in AAA Development and the Rupture., Kugo H, Tanaka H, Moriyama T, Zaima N, Annals of vascular diseases, 11(2), 159 - 168, Jun. 2018 , Refereed
  • Anti-osteoporotic effects of syringic acid and vanilic acid in the extracts of waste beds after mushroom cultivation., Tanaka T, Onuma H, Shigihara T, Kimura E, Fukuta Y, Shirasaka N, Moriyama T, Homma Y, Journal of bioscience and bioengineering, Jun. 2019 , Refereed
  • Using Turmeric Oil as a Solvent Improves the Distribution of Sesamin-Sesamolin in the Serum and Brain of Mice., Iwamoto K, Matsumura S, Yoshioka Y, Yamamoto A, Makino S, Moriyama T, Zaima N, Lipids, 54(5), 311 - 320, May 2019 , Refereed
  • Food-dependent exercise-induced anaphylaxis due to pickled Japanese apricot., Yamanaka M, Kato A, Moriyama T, Okazaki F, Momma K, Narita H, Allergology international : official journal of the Japanese Society of Allergology, Mar. 2019 , Refereed
  • Sesame Extract Attenuates the Degradation of Collagen and Elastin Fibers in the Vascular Walls of Nicotine-administered Mice., Kugo H, Miyamoto C, Sawaragi A, Hoshino K, Hamatani Y, Matsumura S, Yoshioka Y, Moriyama T, Zaima N, Journal of oleo science, 68(1), 79 - 85, 2019 , Refereed
  • Identification of the 7S and 11S globulins as percutaneously sensitizing soybean allergens as demonstrated through epidermal application of crude soybean extract, Murakami H, Ogawa T, Takafuta A, Yano E, Zaima N, Moriyama T, Bioscience, Biotechnology, and Biochemistry, 82(8), 1 - 9, Apr. 2018 , Refereed
  • Dietary DNA Attenuates the Degradation of Elastin Fibers in the Aortic Wall in Nicotine-Administrated Mice., Hashimoto K, Zaima N, Sekiguchi H, Kugo H, Miyamoto C, Hoshino K, Kawasaki N, Sutoh K, Usumi K, Moriyama T, Journal of nutritional science and vitaminology, 64(4), 271 - 276, 2018 , Refereed
  • Worm wounding increases levels of pollen-related food allergens in soybean (Glycine max), Kayo Hanafusa, Hiroki Murakami, Takuya Ueda, Erika Yano, Nobuhiro Zaima, Tatsuya Moriyama, Bioscience, Biotechnology, and Biochemistry, 82(7), 1 - 9, Apr. 2018 , Refereed
  • Effect of a High-sucrose Diet on Abdominal Aortic Aneurysm Development in a Hypoperfusion-induced Animal Model., Miyamoto C, Kugo H, Hashimoto K, Sawaragi A, Zaima N, Moriyama T, Journal of oleo science, 67(5), 589 - 597, Apr. 2018 , Refereed
  • Visualization of the distribution of anthocyanin species in mice eyeball by matrix-assisted laser desorption/ionization mass spectrometry imaging., Yamada S, Zaima N, Yoshimura Y, Inaba S, Fujimori T, Sogon T, Moriyama T, Rapid communications in mass spectrometry : RCM, 32(5), 380 - 384, Mar. 15 2018 , Refereed
  • The Effect of a High-Fat Diet on the Development of Abdominal Aortic Aneurysm in a Vascular Hypoperfusion-Induced Animal Model., Hashimoto K, Kugo H, Tanaka H, Iwamoto K, Miyamoto C, Urano T, Unno N, Hayamizu K, Zaima N, Moriyama T, Journal of vascular research, 55(2), 63 - 74, Jan. 2018 , Refereed
  • Adipocytes and abdominal aortic aneurysm: Putative potential role of adipocytes in the process of AAA development., Kugo H, Moriyama T, Zaima N, Current drug targets, 19(11), 1228 - 1232, Jan. 2018 , Refereed
  • Appearance of Adipocytes in Thoracic Aortic Aneurysm., Kugo H, Ikeda Y, Moriyama T, Zaima N, Journal of oleo science, 67(12), 1543 - 1549, 2018 , Refereed
  • Percutaneous Sensitization to Soybean Proteins Is Attenuated by Oral Tolerance., Murakami H, Ogawa T, Takafuta A, Yano E, Zaima N, Moriyama T, Journal of nutritional science and vitaminology, 64(6), 483 - 486, 2018 , Refereed
  • Group B Soyasaponin Aglycone Suppresses Body Weight Gain and Fat Levels in High Fat-Fed Mice., Kamo S, Takada Y, Yamashita T, Sato T, Yano E, Zaima N, Moriyama T, Journal of nutritional science and vitaminology, 64(3), 222 - 228, 2018 , Refereed
  • Antiosteoporotic activity of a syringic acid diet in ovariectomized mice, Tanaka T, Kawaguchi N, Zaima N, Moriyama T, Fukuta Y, Shirasaka N., Journal of Natural Medicines, 71(4), 1 - 10, Oct. 2017 , Refereed
  • フキノトウアレルギー 11例の検討, 矢口 順子, 角田 孝彦, 森山 達哉, 鈴木 民夫, 皮膚病診療, 39(10), 1040 - 1043, Oct. 2017
    Summary:2002年〜2014年にフキノトウアレルギー患者11例を対象に、検討した。診断はフキノトウ摂取後に蕁麻疹や口腔アレルギー症候群(OAS)などの症状を呈し、フキノトウによるプリックテスト(PT)陽性の場合とした。一部の患者では雄株・雌株それぞれの茎、小花の部位で分けテストを行った。PT判定は15分後、膨疹の大きさ「(最長径+その中点に垂直な径)/2)」を測定し、陽性コントロール(10mg/mlの二塩酸ヒスタミン水溶液)の2倍以上を4、同等以上を3、1/2以上を2+、1/2より小さく陰性コントロール(生理食塩水)より大きいものを1+、陰性コントロールと同等を-とし、3mm以上の膨疹で、2+以上を陽性とした。健常者2名で同様にPTを行い陰性を確認した。患者年齢は21〜58歳、平均39歳、男女比は男性3名、女性8名、全例で蕁麻疹を認め、OASが次いで多く、5例でアナフィラキシー症状を認めた。検討した11例において、臨床症状、花粉待機的IgE抗体、ウエスタンブロットで検出されたIgEバンドの分子量に関連性は見いだせなかった。
  • Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice, Kugo H, Zaima N, Onozato M, Miyamoto C, Hashimoto K, Yanagimoto K, Moriyama T., Food and Function, 8(8), 2829 - 2835, Aug. 01 2017 , Refereed
  • Pathological analysis of the ruptured vascular wall of hypoperfusion-induced abdominal aortic aneurysm animal model, Kugo H, Zaima N, Tanaka H, Hashimoto K, Miyamoto C, Sawaragi A, Urano T, Unno N, Moriyama T., Journal of Oleo Science, 66(5), 499 - 506, May 2017 , Refereed
  • Hyperglycemia Suppresses RANKL-Induced Osteoclast Differentiation through LXR beta Expression in RAW264.7 Cells, Tanaka Teruyoshi, Takei Yuichiro, Zaima Nobuhiro, Moriyama Tatsuya, Yamanouchi Dai, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 63(1), 28 - 34, Feb. 2017 , Refereed
  • Detection of pollinosis-related soybean allergen, Gly m 3 (Profilin) in the various soybean-processed foods., MORIYAMA Tatsuya, The Allergy in Practice, 37(1), 58 - 61, Jan. 2017 , Refereed
  • The effects of nicotine administration on the pathophysiology of rat aortic wall, Kugo H, Zaima N, Tanaka H, Urano T, Unno N, Moriyama T., Biotechnic and Histochemistry, 92(2), 141 - 148, 2017 , Refereed
  • Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice, Kugo H, Zaima N, Onozato M, Miyamoto C, Hashimoto K, Yanagimoto K, Moriyama T., Food and Function, 8(8), 2829 - 2835, 2017 , Refereed
  • Hyperglycemia suppresses RANKL-induced osteoclast differentiation through LXRβ expression in RAW264.7 cells, Tanaka T, Takei Y, Zaima N, Moriyama T, Yamanouchi D., Journal of Nutritional Science and Vitaminology, 63(1), 28 - 34, 2017 , Refereed
  • Puerarin suppresses macrophage activation via antioxidant mechanisms in a CaPO4-induced mouse model of aneurysm, Tanaka T, Moriyama T, Kawamura Y, Yamanouchi D., Journal of Nutritional Science and Vitaminology, 62(6), 425 - 431, Dec. 2016 , Refereed
  • Anti-hyperglycemic effect of a kudzu (Pueraria lobata) vine extract in ovariectomized mice, Tanaka T, Yokota Y, Tang H, Zaima N, Moriyama T, Kawamura Y., Journal of Nutritional Science and Vitaminology, 62(5), 349 - 349, Oct. 2016 , Refereed
  • Significant advancement of mass spectrometry imaging for food chemistry, Yoshimura Yukihiro, Goto-Inoue Naoko, Moriyama Tatsuya, Zaima Nobuhiro, FOOD CHEMISTRY, 210, 200 - 211, Nov. 01 2016 , Refereed
  • The anti-obesity effect of Taheebo (Tabebuia avellanedae Lorentz ex Griseb) extract in ovariectomized mice and the identification of a potential anti-obesity compound., Iwamoto K, Fukuda Y, Tokikura C, Noda M, Yamamoto A, Yamamoto M, Yamashita M, Zaima N, Iida A, Moriyama T, Biochemical and biophysical research communications, 478(3), 1136 - 1140, Sep. 2016 , Refereed
  • Trials of the quantification of soybean allergen, Gly m4 by competitive ELISA methods, MORIYAMA Tatsuya, The Allergy in Practice, 36(9), 64 - 68, Aug. 2016 , Refereed
  • Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm, Kugo H, Zaima N, Tanaka H, Mouri Y, Yanagimoto K, Hayamizu K, Hashimoto K, Sasaki T, Sano M, Yata T, Urano T, Setou M, Unno N, Moriyama T., Scientific Reports, 6, 2016 , Refereed
  • The preventive effect of fish oil on abdominal aortic aneurysm development, Hirona Kugo, Nobuhiro Zaima, Youhei Mouri, Hiroki Tanaka, Kenichi Yanagimoto, Tetsumei Urano, Naoki Unno, Tatsuya Moriyama, Bioscience, Biotechnology and Biochemistry, 80, 1186 - 1191, Jan. 01 2016
    Summary:� 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry. Abdominal aortic aneurysm (AAA) is a vascular disease involving gradual dilation of the abdominal aorta and high rupture-related mortality rates. AAA is histologically characterized by oxidative stress, chronic inflammation, and extracellular matrix degradation in the vascular wall. We previously demonstrated that aortic hypoperfusion could cause the vascular inflammation and AAA formation. However, the preventive method for hypoperfusion-induced AAA remains unknown. In this study, we evaluated the effect of fish oil on AAA development using a hypoperfusion-induced AAA animal model. Dilation of the abdominal aorta in the fish oil administration group was smaller than in the control group. Collagen destruction and oxidative stress were suppressed in the fish oil administration group than in the control group. These results suggested that fish oil could prevent the development of AAA induced by hypoperfusion.
  • Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium, Zaima N, Kinoshita S, Hieda N, Kugo H, Narisawa K, Yamamoto A, Yanagimoto K, Moriyama T., Biochemistry and Biophysics Reports, 7, 259 - 265, 2016 , Refereed
  • Inhibitory activities of essential oil obtained from turmeric and its constituents against β-secretase, Matsumura S, Murata K, Zaima N, Yoshioka Y, Morimoto M, Kugo H, Yamamoto A, Moriyama T, Matsuda H., Natural Product Communications, 11(12), 1785 - 1788, 2016 , Refereed
  • Immediate allergy due to raw garlic (Allium sativum L.)., Yagami A, Suzuki K, Sano A, Iwata Y, Arima M, Moriyama T, Matsunaga K, The Journal of dermatology, 42(10), 1026 - 1027, Oct. 2015 , Refereed
  • Variability analysis of pan-allergen (PR-5,PR-10, profilin) levels of plant-foods infected by fungi., MORIYAMA Tatsuya, 35(9), 876 - 879, Sep. 2015 , Refereed
  • Hypoperfusion of the Adventitial Vasa Vasorum Develops an Abdominal Aortic Aneurysm., Tanaka H, Zaima N, Sasaki T, Sano M, Yamamoto N, Saito T, Inuzuka K, Hayasaka T, Goto-Inoue N, Sugiura Y, Sato K, Kugo H, Moriyama T, Konno H, Setou M, Unno N, PloS one, 10(8), e0134386, 2015 , Refereed
  • Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall., Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Sano M, Saito T, Hayasaka T, Goto-Inoue N, Sato K, Kugo H, Moriyama T, Konno H, Setou M, Unno N, Journal of vascular research, 52(2), 127 - 135, 2015 , Refereed
  • Diversity of Food Allergy., Moriyama T, Journal of nutritional science and vitaminology, 61 Suppl, S106 - 8, 2015 , Refereed
  • Variability analysis of resistin involved in inflammation and allergy, MORIYAMA Tatsuya, The Allergy in Practice, 35(14), 58 - 61, 2015 , Refereed
  • Distribution of lysophosphatidylcholine in the endosperm of Oryza sativa rice., Zaima N, Yoshimura Y, Kawamura Y, Moriyama T, Rapid communications in mass spectrometry : RCM, 28(13), 1515 - 1520, Jul. 15 2014 , Refereed
  • Enhanced specificity for phosphatidylcholine analysis by positive ion mode matrix-assisted laser desorption/ionization imaging mass spectrometry., Zaima N, Yoshioka S, Sato Y, Shinano S, Ikeda Y, Moriyama T, Rapid communications in mass spectrometry : RCM, 28(13), 1453 - 1458, Jul. 15 2014 , Refereed
  • Identification of peamaclein as a marker allergen related to systemic reactions in peach allergy., Inomata N, Okazaki F, Moriyama T, Nomura Y, Yamaguchi Y, Honjoh T, Kawamura Y, Narita H, Aihara M, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 112(2), 175 - 177.e3, Feb. 2014 , Refereed
  • Molecular cloning and expression profiling of procollagen α1 (I) of cultured Pacific bluefin tuna, Teruyoshi Tanaka, Teruyoshi Tanaka, Kenji Takahashi, Kohsuke Adachi, Haruki Ohta, Yukihiro Yoshimura, Yasuo Agawa, Yoshifumi Sawada, Osamu Takaoka, Amal Kumar Biswas, Kenji Takii, Nobuhiro Zaima, Tatsuya Moriyama, Yukio Kawamura, Yukio Kawamura, Fisheries Science, 80, 603 - 612, Jan. 01 2014
    Summary:Type I collagen is widely distributed in most organs in teleosts. It plays a role not only in intercellular adhesion, but also in molecular signaling. In this study, Pacific bluefin tuna (PBT) procollagen α1 (I) cDNA was cloned and characterized. The nine fragments of a procollagen α1 (I) chain cDNA clone were prepared and spliced together to create the complete coding region. The resulting amino acid sequence was homologous with that of other teleosts. The mRNA expression profile of PBT procollagen α1 (I) in various tissues and the phylogenetic analysis with other vertebrate procollagen α1 (I) chains suggest that PBT procollagen α1 (I) could be a precursor form of the PBT type I collagen α1 chain. In addition, its level of expression in PBT larvae and early juveniles gradually increased with somatic growth. This increase was related to the standard length, wet body weight, and protein content of each individual fish. Therefore, the expression profile of procollagen α1 (I) may be a useful indicator for somatic growth in fish larvae and juveniles. © 2014 The Japanese Society of Fisheries Science.
  • Matrix-assisted laser desorption/ionization imaging mass spectrometry: new technology for vascular pathology., Zaima N, Goto-Inoue N, Moriyama T, Journal of vascular research, 51(2), 144 - 148, 2014 , Refereed
  • Ellagic acid improves hepatic steatosis and serum lipid composition through reduction of serum resistin levels and transcriptional activation of hepatic ppara in obese, diabetic KK-A(y) mice., Yoshimura Y, Nishii S, Zaima N, Moriyama T, Kawamura Y, Biochemical and biophysical research communications, 434(3), 486 - 491, May 2013 , Refereed
  • Hypoallergenicity and immunological characterization of enzyme-treated royal jelly from Apis mellifera., Moriyama T, Yanagihara M, Yano E, Kimura G, Seishima M, Tani H, Kanno T, Nakamura-Hirota T, Hashimoto K, Tatefuji T, Ogawa T, Kawamura Y, Bioscience, biotechnology, and biochemistry, 77(4), 789 - 795, 2013 , Refereed
  • Effect of gamma irradiation on soybean allergen levels., Moriyama T, Yano E, Kitta K, Kawamoto S, Kawamura Y, Todoriki S, Bioscience, biotechnology, and biochemistry, 77(12), 2371 - 2377, 2013 , Refereed
  • Hypoallergenicity of various miso pastes manufactured in Japan., Moriyama T, Yano E, Suemori Y, Nakano K, Zaima N, Kawamura Y, Journal of nutritional science and vitaminology, 59(5), 462 - 469, 2013 , Refereed
  • A case of an allergic reaction due to Anisakis simplex possibly after the ingestion of squid - Successful detection of four A. simplex allergens, Ani s 1, Ani s 2, Ani s 12 and troponin C-like protein, Shigeruko Iijima, Tatsuya Moriyama, Hidetaka Ichikawa, Yukihiro Kobayashi, Kazuo Shiomi, Japanese Journal of Allergology, 61, 1104 - 1110, Oct. 09 2012
    Summary:A 62-year-old man ingested dressed salmon and its roe (ikura) and grilled mackerel and one hour later further ingested raw tuna and squid as an evening meal at a bar. Soon after the ingestion of raw seafood, he showed wheals, loss of consciousness and low blood pressure. Specific serum IgE to the nematode Anisakis simplex was positive but those to some seafoods were negative. Moreover, a skin prick test using the crude extract was positive for A. simplex but negative for the seafoods, which he ingested on the day of the above episode. When the A. simplex extract was analyzed by IgE-binding immunoblot analysis using the patient serum, two highly intense protein bands were recognized at 18 and 17kDa, one intense band at 35kDa and two weak bands at 28 and 26kDa. ELISA with 11 natural or recombinant A. simplex allergens (Ani s 1-6, 8, 9, 11 and 12 and troponin C-like protein) showed that the patient serum strongly reacted to Ani s 1 and Ani s 12 and weakly to Ani s 2 and troponin C-like protein. Based on these results, he was diagnosed as IgE-mediated A. simplex allergy due to four allergens (Ani s 1, Ani s 2, Ani s 12 and troponin C-like protein), possibly infested in the raw squid which he had ingested just before manifestation of allergic reactions. ©2012 Japanese Society of Allergology.
  • Hepatoprotective action of dietary bluefin tuna skin proteins on CCl4-intoxicated mice, Teruyoshi Tanaka, Kenji Takahashi, Naoki Iwamoto, Yasuo Agawa, Yoshifumi Sawada, Yukihiro Yoshimura, Nobuhiro Zaima, Tatsuya Moriyama, Yukio Kawamura, Fisheries Science, 78, 911 - 921, Jul. 01 2012
    Summary:We have shown that dietary bluefin tuna skin (TUS) protects against carbon tetrachloride (CCl 4 )-induced hepatic damage in mice. The CCl 4 -induced necrotic area was decreased in mice fed a TUS-containing diet. Consistent with the decreased necrotic area, dietary TUS markedly lowered the elevated serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and the thiobarbituric acid-reactive substance (TBARS) formation induced by CCl 4 injection. TUS diets also decreased phosphorylation of inhibitory kappa B-α and blocked the translocation of nuclear factor-kappa B to the nucleus. TUS is composed mainly (80.7 %) of type I collagen, and our results revealed that dietary tuna collagen peptides (TUCP) attenuated the increased hepatic necrotic area, serum AST and ALT activities, and liver TBARS levels induced by CCl 4 , similar to TUS, thus enabling us to attribute the hepatoprotective action of TUS in CCl 4 -intoxicated mice to tuna collagen. Therefore, TUS and TUCP may be potential food resources that are capable of alleviating hepatitis symptoms. © 2012 The Japanese Society of Fisheries Science.
  • Puerarin exerted anti-osteoporotic action independent of estrogen receptor-mediated pathway., Michihara S, Tanaka T, Uzawa Y, Moriyama T, Kawamura Y, Journal of nutritional science and vitaminology, 58(3), 202 - 209, Jun. 2012 , Refereed
  • Visualization of anthocyanin species in rabbiteye blueberry Vaccinium ashei by matrix-assisted laser desorption/ionization imaging mass spectrometry., Yoshimura Y, Enomoto H, Moriyama T, Kawamura Y, Setou M, Zaima N, Analytical and bioanalytical chemistry, 403(7), 1885 - 1895, Jun. 2012 , Refereed
  • Importance of Specific IgE to Gly m4, Gly m5, and Gly m6 and Subunits of Gly m5 and Gly m6 in Patients with Soybean Allergy, ADACHI Atsuko, MORIYAMA Tatsuya, SHIMIZU Hideki, HORIKAWA Tatsuya, TANAKA Akira, SJOLANDER Sigrid, Journal of environmental dermatology and cutaneous allergology, Journal of environmental dermatology and cutaneous allergology = / the Japanese Society for Dermatoallergology and Contact Dermatitis, 6(2), 60 - 66, Apr. 30 2012
  • Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry., Yoshimura Y, Zaima N, Moriyama T, Kawamura Y, PloS one, 7(2), Feb. 17 2012 , Refereed
  • Ellagic acid in pomegranate suppresses resistin secretion by a novel regulatory mechanism involving the degradation of intracellular resistin protein in adipocytes., Makino-Wakagi Y, Yoshimura Y, Uzawa Y, Zaima N, Moriyama T, Kawamura Y, Biochemical and biophysical research communications, 417(2), 880 - 885, Jan. 2012 , Refereed
  • Catechins and Caffeine Inhibit Fat Accumulation in Mice through the Improvement of Hepatic Lipid Metabolism., Sugiura C, Nishimatsu S, Moriyama T, Ozasa S, Kawada T, Sayama K, Journal of obesity, 2012, 520510, 2012 , Refereed
  • Kudzu (Pueraria lobata) vine ethanol extracts improve ovariectomy-induced bone loss in female mice., Tanaka T, Tang H, Yu F, Michihara S, Uzawa Y, Zaima N, Moriyama T, Kawamura Y, Journal of agricultural and food chemistry, 59(24), 13230 - 13237, Dec. 28 2011 , Refereed
  • Immunoblotting Analysis of nsLTP1 in Cereal Grains with Antiserum Raised against Recombinant Rice nsLTP1, Gang Hua Lang, Yukari Kagiya, Mayumi Ohnishi-Kameyama, Shinichi Kawamoto, Tatsuya Moriyama, Kazumi Kitta, Food Science and Technology Research, 17, 499 - 504, Dec. 01 2011
    Summary:Nonspecific lipid transfer protein 1 (nsLTP1) is widely distributed in plants and has been recognized as a widely cross-reacting plant pan-allergen. In the present study, a recombinant protein of rice nsLTP1 was prepared with an Escherichia coli expression system, and polyclonal antiserum was raised by immunizing rabbits with this recombinant nsLTP1. The antiserum specifically immunoreacted with rice nsLTP1 and homologous nsLTP1 proteins of other kinds of cereal grains. Moreover, the antiserum was applied to compare nsLTP1 content among rice cultivars and among rice fractions separated by grain-milling. Variation of nsLTP1 contents were observed among rice cultivars, and rice nsLTP1 was proved to be mainly located in the outer layer of rice grains, providing evidence for decreasing nsLTP1 content by grain-milling.
  • Major royal jelly protein 3 as a possible allergen in royal jelly-induced anaphylaxis., Mizutani Y, Shibuya Y, Takahashi T, Tsunoda T, Moriyama T, Seishima M, The Journal of dermatology, 38(11), 1079 - 1081, Nov. 2011 , Refereed
  • Importance of Specific IgE to Gly m4, Gly m3 in Patients with Soymilk Allergy, ADACHI Atsuko, MORIYAMA Tatsuya, HARADA Susumu, FUKUNAGA Atsushi, HORIKAWA Tatsuya, TANAKA Akira, SJORANDER Sigrid, Journal of environmental dermatology and cutaneous allergology, Journal of environmental dermatology and cutaneous allergology = / the Japanese Society for Dermatoallergology and Contact Dermatitis, 5(5), 431 - 438, Oct. 31 2011
  • Two cases of royal jelly allergy provoked the symptoms at the time of their first intake, Susumu Harada, Tatsuya Moriyama, Akira Tanaka, Japanese Journal of Allergology, 60, 708 - 713, Aug. 01 2011
    Summary:Two young women were suffered from several symptoms after the intake of royal jelly at their first time. According to the positive skin prick test reactions of raw royal jelly, royal jelly allergy was diagnosed. As the reasons why the symptoms appeared at the time of their first intake, we guessed the possibility that 1) they had been sensitized for royal jelly formerly, or 2) their symptoms were induced by the cross-reactivity between royal jelly and other allergens such as bee, honey and pollens. As to our cases, no related allergens were found in one case, but in another case co-existance of mugwort allergy was suspected from the results of both skin prick test and specific IgE titers. Originally royal jelly allergy has been regarded as class 1 allergic reaction developed by the sensitization of itself. But we speculated the possibility that there can also be cases of class 2 royal jelly allergy by the mechanism of cross-reaction with pollens. ©2011 Japanese Society of Allergology.
  • Activation of peroxisome proliferator-activated receptor-alfa (PPARalfa) suppresses postprandial lipidemia through fatty acid oxidation in enterocyte, KIMURA Rino, TAKAHASHI Nobuyuki, MUROTA Kaeko, YAMADA Yuko, NIIYA Saori, KANZAKI Noriyuki, MURAKAMI Yoko, MORIYAMA Tatsuya, GOTO Tsuyoshi, KAWADA Teruo, Biochem Biophys Res Commun, 410(1), 1 - 6, Jun. 24 2011 , Refereed
  • Immunological characterization of polyclonal antisera prepared against recombinant rice RAG2 and its application in detection of 14-16 kDa α-amylase/trypsin inhibitors from processed foods, Gang Hua Lang, Mika Ohba, Shinichi Kawamoto, Koichi Yoza, Tatsuya Moriyama, Kazumi Kitta, Food Science and Technology Research, 16, 599 - 606, Nov. 01 2010
    Summary:RAG2 is a member of the rice 14-16 kDa α-amylase/trypsin inhibitors, which are the main allergens in rice grains. Recombinant RAG2 protein was expressed in Escherichia coli, and polyclonal antisera were prepared against the protein. The polyclonal antisera specifically bound with 14-16 kDa a-amylase/ trypsin inhibitors from husked brown, red and black rice. No cross-reaction was observed between the polyclonal antisera and saline-soluble proteins from other tested cereal grains. By immunoblotting with the polyclonal antisera, 14-16 kDa α-amylase/trypsin inhibitors were found to be distributed throughout the endosperm of brown rice and their contents were estimated. Moreover, 14-16 kDa a-amylase/trypsin inhibitors were also detected in processed foods. Thus, the polyclonal antisera prepared against recombinant RAG2 protein could be used to compare the content of 14-16 kDa a-amylase/trypsin inhibitors in rice samples and probe the presence of the allergen in processed foods.
  • Food-dependent anaphylaxis with serum IgE immunoreactive to dairy products containing high-molecular-weight proteins., Abe S, Kabashima K, Moriyama T, Tokura Y, Journal of dermatological science, 57(2), 137 - 140, Feb. 2010 , Refereed
  • Effect of gamma-irradiation on the survival of Listeria monocytogenes and allergenicity of cherry tomatoes, Todoriki Setsuko, Bari Latiful, Kitta Kazumi, Ohba Mika, Ito Yasuhiro, Tsujimoto Yuka, Kanamori Norihito, Yano Erika, Moriyama Tatsuya, Kawamura Yukio, Kawamoto Shinichi, RADIATION PHYSICS AND CHEMISTRY, 78(7-8), 619 - 621, Jul. 2009 , Refereed
  • Anaphylaxis due to peach with negative ImmunoCAP result to peach allergens, including rPru p 1, rPru p 3 and rPru p 4: A report of two cases, Nobuko Maeda, Naoko Inomata, Akiko Morita, Mio Kirino, Tatsuya Moriyama, Zenro Ikezawa, Japanese Journal of Allergology, 58, 140 - 147, Apr. 24 2009
    Summary:We report two cases of anaphylactic reactions to peach with negative result of ImmunoCAP to peach. Case 1 is a 35-year-old man, who felt an itch in his oral cavity immediately after ingesting a whole fresh peach. He rapidly developed generalized urticaria, dyspnea, vomiting, and loss of consciousness. He recovered after treatment at a local hospital, thereafter he was referred to our hospital because ImmunoCAP conducted for screening allergens revealed a negative test result to peach and the cause of anaphylaxis remained unclear. He had a history of pollinosis. He reported that he previously felt an itch on his oral cavity after ingesting melon, watermelon, apple, and strawberry. Serum total IgE was 436IU/ml. CAP-RAST revealed negative results to peach, strawberry and kiwi. Skin prick tests (SPTs) with raw peach pulp, canned peach pulp, strawberry and kiwi were positive. Case 2 is a 30-year-old woman who felt an itch on her oral cavity accompanied by blepharedema, rhinorrhea, generalized urticaria, nausea, abdominal pain and diarrhea after eating peach. She had a history of pollinosis. She reported that she previously developed urticaria after ingesting an apple. Serum total IgE was 85IU/ ml. ImmunoCAP revealed negative results to peach and apple. SPTs with canned yellow peach, strawberry and apple were positive. Consequently, the two patients were diagnosed with anaphylaxis due to peach, and allergic symptoms have never recurred since they avoided ingesting peach. Furthermore, in two patients ImmunoCAP to rPru p 1, rPru p 3, and rPru p 4 were negative. However, in IgE-immunoblotting of peach, serum IgE antibodies of two patients were bound to approximately 10kDa proteins. Meanwhile, the cross-reactivity between Rosaceae fruits, such as peach, apple, apricot, and plum, has been reported. These results suggest that in patients, who are suspected of having peach anaphylaxis and show a negative ImmunoCAP result to peach, the additional testing, such as SPT with peach, should be performed for diagnosis. © 2009 Japanese Society of Allergology.
  • Effects of Insect Control Treatments and Damage Caused by Stored-product Insects on Rice Allergenic Proteins, OHBA Mika, MIYANOSHITA Akihiro, MORIYAMA Tatsuya, KAWAMOTO Shinichi, KITTA Kazumi, NIPPON SHOKUHIN KOGYO GAKKAISHI, 日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology, 56(4), 249 - 253, Apr. 15 2009
    Summary:The number of food allergy sufferers has been increasing and foods containing allergenic proteins have caused concern for many of the people especially in developed countries. Although rice, a staple food for Japanese people, rarely induces severe allergic symptoms such as anaphylactic shock, it has long been known to be a potential cause of atopic dermatitis. We investigated the effects of high-pressure carbon dioxide treatment, an alternative to methyl bromide fumigation for insect pest control, as well as both methyl bromide and phosphine fumigation, on rice allergenic proteins. In addition, brown rice samples were treated with the larvae of the stored-product insect Plodia interpunctella to determine the effects of insect damage on these proteins. We found that neither these insect control treatments nor damage caused by stored-product insect have any effect on allergenic proteins.
  • Changes in lipid metabolism by soy beta-conglycinin-derived peptides in HepG2 cells., Mochizuki Y, Maebuchi M, Kohno M, Hirotsuka M, Wadahama H, Moriyama T, Kawada T, Urade R, J Agric Food Chem., 57(4), 1473 - 1480, Feb. 2009 , Refereed
  • Soybean β-conglycinin as the main allergen in a patient with food-dependent exercise-induced anaphylaxis by tofu: Food processing alters pepsin resistance, A. Adachi, A. Adachi, T. Horikawa, H. Shimizu, Y. Sarayama, T. Ogawa, S. Sjolander, A. Tanaka, T. Moriyama, Clinical and Experimental Allergy, 39, 167 - 173, Jan. 01 2009
    Summary:Background: Food-dependent exercise-induced anaphylaxis (FDEIA) due to soybeans is a rare disorder. The allergen responsible for FDEIA due to soybeans has not yet been determined. Objective: We characterized the clinical features of a patient with FDEIA due to tofu, who was well tolerant to drinking soy milk. We then sought to identify the responsible soybean allergen(s) in that patient. We further studied whether different stabilities of the allergen(s) to pepsin digestion between two soybean products are related to their clinical allergenicity. Methods: Skin prick tests and provocation tests using soybean products were performed to detect the responsible food and other factors that induced the allergic symptoms. Specific IgE to various soybean allergens were examined by ImmunoCAP®, ELISA and protein microarray assays. Immunoblotting for soybeans and soybean products using the patient's serum was also performed. Soybean products were serially digested by pepsin to disclose the stability of the allergens. Results: Provocation with ingestion of tofu and exercise induced the allergic symptoms, while ingestion of soy milk and exercis e did not. Immunoblot analysis, ELISA and protein microarray assay revealed that β-conglycinin mainly reacts with IgE antibodies in the patient's serum. By immunoblot analysis, β-conglycinin in soy milk completely disappeared after pepsin digestion within 20 min, whereas β-conglycinin in tofu was almost intact after more than 120 min of pepsin digestion. Conclusion: We identified β-conglycinin as the causative allergen in a patient with FDEIA induced by tofu. The difference in resistance to pepsin digestion between tofu and soy milk suggests that the presence of undigested allergens in the digestive tract is a prerequisite for the development of FDEIA. © 2008 The Authors.
  • Reliable enzyme-linked immunosorbent assay for the determination of soybean proteins in processed foods., Morishita N, Kamiya K, Matsumoto T, Sakai S, Teshima R, Urisu A, Moriyama T, Ogawa T, Akiyama H, Morimatsu F, Journal of agricultural and food chemistry, 56(16), 6818 - 6824, Aug. 27 2008 , Refereed
  • Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP., Hosokawa N, Wada I, Nagasawa K, Moriyama T, Okawa K, Nagata K, The Journal of biological chemistry, 283(30), 20914 - 20924, Jul. 25 2008 , Refereed
  • Two cases of apiaceae spice allergy, Susumu Harada, Akiko Matsunaga, Rieko Miyachi, Taro Masaki, Tatsuya Moriyama, Japanese Journal of Allergology, 56, 1515 - 1521, Dec. 01 2007
    Summary:Background: Many cases of spice allergy have been reported especially from Scandinavian countries, but in contrast there are few reports in Japan. This time we experienced two cases of apiaceae spice allergy and practiced some kinds of examinations. We report here these two cases with the consideration concerning mechanizm of spice allergy. Methods and Subjects: We practiced 1) specific IgE of pollens and foods, 2) prick tests of spices and apiaceae vesitables, 3) immunoblot of spices, against two cases suspected spice allergy from their clinical courses. Clinically Case 1 32 y.o. male had been no history of pollinosis, in contrast Case 2 46 y.o. female had been suffered from pollinosis during spring and autumn seasons. Results: In Case 1 the scores of specific IgE of pollens were almost negative and immunoblot examination of spices revealed positive reaction at the site of 10∼12kDa and 60kDa. In Case 2 the scores of specific IgE of pollens were positive in many species and immunoblot examination of spices reacted positively at the site of 14kDa and 60kDa. Both of them showed positive reactions against many kinds of apiaceae spices in prick tests, so we diagnosed them as apiaceae spice allergy. Conclusion: According to these results we suspected Case 1 as class 1 allergy induced by the sensitization of spices themselvs and Case 2 as class 2 allergy caused by the cross reactions with pollinosis. So there may be some different mechanizms in the occurrence of spice allergy. In the future the occurrence of spice allergy will be supposed to increase and it will be necessary for us to pay much more attention to spice allergy even in Japan.
  • Detection of Food Allergens Using Near-infrared Fluorescent Probes, Moriyama Tatsuya, Mitsuyama Hideyoshi, Yano Erika, Ohba Mika, Kitta Kazumi, Kawamoto Shin-Ichi, Akiyama Hiroshi, Urisu Atsuo, Takahashi Koji, Hajika Makita, Ogawa Tadashi, Kawamura Yukio, JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 54(11), 468 - 476, Nov. 2007 , Refereed
  • Specific detection of soybean residues in processed foods by the polymerase chain reaction., Yamakawa H, Akiyama H, Endo Y, Miyatake K, Sakata K, Sakai S, Moriyama T, Urisu A, Maitani T, Bioscience, biotechnology, and biochemistry, 71(1), 269 - 272, Jan. 2007 , Refereed
  • Changes in hepatic gene expression associated with the hypocholesterolaemic activity of royal jelly, Kamakura M, Moriyama T, Sakaki T, J. Pharm. Pharmacol., 58(12), 1683 - 1689, Dec. 2006 , Refereed
  • Sodium cromoglycate inhibits absorption of the major soybean allergen, Gly m Bd 30K, in mice and human intestinal Caco-2 cells., Weangsripanaval T, Murota K, Murakami Y, Kominami M, Kusudo T, Moriyama T, Ogawa T, Kawada T, The Journal of nutrition, 136(11), 2874 - 2880, Nov. 2006 , Refereed
  • Reduction of allergenic proteins by the effect of the ripening inhibitor (rin) mutant gene in an F1 hybrid of the rin mutant tomato., Kitagawa M, Moriyama T, Ito H, Ozasa S, Adachi A, Yasuda J, Ookura T, Inakuma T, Kasumi T, Ishiguro Y, Ito Y, Bioscience, biotechnology, and biochemistry, 70(5), 1227 - 1233, May 2006 , Refereed
  • Detection of low molecular weight allergens resolved on two-dimensional electrophoresis with acid-urea polyacrylamide gel, Kitta K, Ohnishi-Kameyama M, Moriyama T, Ogawa T, Kawamoto S, Anal. Biochemistry,, 351(2), 290 - 297, Apr. 2006 , Refereed
  • Phytol directly activates peroxisome proliferator-activated receptor alpha (PPARalpha) and regulates gene expression involved in lipid metabolism in PPARalpha-expressing HepG2 hepatocytes., Goto T, Takahashi N, Kato S, Egawa K, Ebisu S, Moriyama T, Fushiki T, Kawada T, Biochemical and biophysical research communications, 337(2), 440 - 445, Nov. 2005 , Refereed
  • Dietary fat and an exogenous emulsifier increase the gastrointestinal absorption of a major soybean allergen, Gly m Bd 30K, in mice., Weangsripanaval T, Moriyama T, Kageura T, Ogawa T, Kawada T, The Journal of nutrition, 135(7), 1738 - 1744, Jul. 2005 , Refereed
  • Influence of royal jelly on mouse hepatic gene expression and safety assessment with a DNA microarray., Kamakura M, Maebuchi M, Ozasa S, Komori M, Ogawa T, Sakaki T, Moriyama T, Journal of nutritional science and vitaminology, 51(3), 148 - 155, Jun. 2005 , Refereed
  • [The structures and functions of peroxisome proliferator-activated receptors (PPARs)]., Takahashi N, Goto T, Kusudo T, Moriyama T, Kawada T, Nihon rinsho. Japanese journal of clinical medicine, 63(4), 557 - 564, Apr. 2005 , Refereed
  • A Novel Enzyme-Linked Immunosorbent Assay for Quantification of Soybean beta-Conglycinin, a Major Soybean Storage Protein, in Soybean and Soybean Food Products, Moriyama T, Machidori M, Ozasa S, Maebuchi M, Urade R, Takahashi K, Ogawa T, Maruyama N, Journal of nutritional science and vitaminology, 51(1), 34 - 39, Feb. 2005 , Refereed
  • ER-60 domains responsible for interaction with calnexin and calreticulin., Urade R, Okudo H, Kato H, Moriyama T, Arakaki Y, Biochemistry, 43(27), 8858 - 8868, Jul. 2004 , Refereed
  • Soybean beta-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of beta-oxidation, downregulation of fatty acid synthase, and inhibition of triglyceride absorption., Moriyama T, Kishimoto K, Nagai K, Urade R, Ogawa T, Utsumi S, Maruyama N, Maebuchi M, Bioscience, biotechnology, and biochemistry, 68(2), 352 - 359, Feb. 2004 , Refereed
  • Molecular and structural analysis of electrophoretic variants of soybean seed storage proteins., Maruyama N, Fukuda T, Saka S, Inui N, Kotoh J, Miyagawa M, Hayashi M, Sawada M, Moriyama T, Utsumi S, Phytochemistry, 64(3), 701 - 708, Oct. 2003 , Refereed
  • Low resistin levels in adipose tissues and serum in high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin., Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T, Archives of biochemistry and biophysics, 416(2), 164 - 170, Aug. 2003 , Refereed
  • Identification of suberization-associated anionic peroxidase as a possible allergenic protein from tomato., Weangsripanaval T, Nomura N, Moriyama T, Ohta N, Ogawa T, Bioscience, biotechnology, and biochemistry, 67(6), 1299 - 1304, Jun. 2003 , Refereed
  • Functions of soy phosphatidylcholine in dough and bread supplemented with soy protein, Reiko Urade, S. Okamoto, T. Yagi, T. Moriyama, T. Ogawa, M. Kito, Journal of Food Science, 68, 1276 - 1282, Jan. 01 2003
    Summary:The loaf volume decreased on the addition of soybean protein isolate (SPI) to bread. When phosphatidylcholine was added to the ingredients, including SPI, the adverse effect of SPI on the loaf volume could be counteracted. The addition of phosphatidylcholine showed little effect on the rheological properties of dough. It was shown by confocal microscopy that phosphatidylcholine was associated with gluten in the dough. The function of phosphatidylcholine could not be replaced by phosphatidylethanolamine, phospatidic acid, or lysophospatidylcholine. Phosphatidylcholine showed different effects depending on the phosphatidylcholine molecular species.
  • Transglutaminase activity of human ER-60., Okudo H, Kito M, Moriyama T, Ogawa T, Urade R, Bioscience, biotechnology, and biochemistry, 66(6), 1423 - 1426, Jun. 2002 , Refereed
  • Identification of cyclophilin as an IgE-binding protein from carrots, Chizuko Fujita, Tatsuya Moriyama, Tadashi Ogawa, Tadashi Ogawa, International Archives of Allergy and Immunology, 125, 44 - 50, Jun. 18 2001
    Summary:Background: Plant food allergies have been associated with pollenosis, although most of the causative allergens are as yet undefined. It is important to elucidate the properties of plant food allergens in order to minimize a patient's risks in food selection. The purpose of the present study was to examine and characterize the IgE-binding proteins in carrots as possible allergens by using patients' sera. Method: IgE-binding proteins in carrot extract were screened by an immunoblot technique using sera of patients with atopic dermatitis (selected based upon a case history of food allergies). An allergenic protein was purified from carrot extract by chromatographic procedures. The N-terminal amino acid sequence of allergenic protein was determined and subjected to a computer homology search. Cross-reactivity between carrot and birch allergens was examined by immunoblotting. Results and Conclusion: A unique, approximately 20-kDa allergenic protein that reacted with about 14% of patients' sera was isolated and characterized. The N-terminal amino acid sequence of the purified protein was found to be homologous with those of plant cyclophilins. This allergen exhibited a peptidyl-prolyl cis-transisomerase activity, which was inhibited by the conjugation of cyclosporin A. These properties of the allergenic protein isolated from carrot identified it as a cyclophilin, a possible plant food allergen. No cross-reactivity between this 20-kDa carrot allergen and Bet v 7, a birch pollen cylcophilin, was observed. Copyright © 2001 S. Karger AG, Basel.
  • 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Is Sterol-Dependently Cleaved by Cathepsin L-type Cysteine Protease in the Isolated Endoplasmic Reticulum., MORIYAMA T, WADA M, URADE R, KITO M, KATUNUMA N, OGAWA T, SIMONI R D, Archives of Biochemistry and Biophysics, 386(2), 205 - 212, Feb. 15 2001
    Summary:We have recently shown that 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, an endoplasmic reticulum (ER) membrane protein, is degraded in ER membranes prepared from sterol pretreated cells and that such degradation is catalyzed by a cysteine protease within the reductase membrane domain. The use of various protease inhibitors suggested that degradation of HMG-CoA reductase in vitro is catalyzed by a cathepsin L-type cysteine protease. Purified ER contains E-64-sensitive cathepsin L activity whose inhibitor sensitivity was well matched to that of HMG-CoA reductase degradation in vitro. CLIK-148 (cathepsin L inhibitor) inhibited degradation of HMG-CoA reductase in vitro. Purified cathepsin L also efficiently cleaved HMG-CoA reductase in isolated ER preparations. To determine whether a cathepsin L-type cysteine protease is involved in sterol-regulated degradation of HMG-CoA reductase in vivo, we examined the effect of E-64d, a membrane-permeable cysteine protease inhibitor, in living cells. While lactacystin, a proteasome-specific inhibitor, inhibited sterol-dependent degradation of HMG-CoA reductase, E-64d failed to do so. In contrast, degradation of HMG-CoA reductase in sonicated cells was inhibited by E-64d, CLIK-148, and leupeptin but not by lactacystin. Our results indicate that HMG-CoA reductase is degraded by the proteasome under normal conditions in living cells and that it is cleaved by cathepsin L leaked from lysosomes during preparation of the ER, thus clarifying the apparently paradoxical in vivo and in vitro results. Cathepsin L-dependent proteolysis was observed to occur preferentially in sterol-pretreated cells, suggesting that sterol treatment results in conformational changes in HMG-CoA reducta se that make it more susceptible to such cleavage. © 2001 Academic Press.
  • Nondestructive Quantification of Neutral Lipids by Thin-Layer Chromatography and Laser-Flurescent Scanning : Suitable Methods for “Lipidome" Analysis., KISHIMOTO K, URADE R, OGAWA T, MORIYAMA T, Biochemical and Biophysical Research Communications, 281(3), 657 - 662, Jan. 01 2001
    Summary:A simple method for separation and quantification of neutral lipids was developed using thin-layer chromatography (TLC) and high-performance fluorescent scanning. Neutral lipid classes were separated using the double-developing TLC method and detected by rhodamine 6G and a laser-excited fluorescent scanner. The amount of lipids applied correlated with scanned intensity volume in a dose-dependent manner. The mass of each neutral lipid band was determined by comparing band intensities of unknown samples to dilution curves of authentic standards. After scanning the dye-sprayed TLC, acyl chain species of triglyceride (TG) extracted from TLC could be determined by gas chromatography. Using this method, we quantified the amounts of TG in mouse liver and found that the measured total mass of TG correlated with that obtained by enzymatic methods. Our method should provide the basic technique for "lipidome" analysis, designed to determine and compare total lipid classes and mass present in biological samples. © 2001 Academic Press.
  • Accumulation and degradation in the endoplasmic reticulum of a truncated ER-60 devoid of C-terminal amino acid residues., Reiko Urade, Masahiko Kusunose, Tatsuya Moriyama, Takahiko Higasa, Makoto Kito, Journal of Biochemistry, 127, 211 - 220, Mar. 18 2000
    Summary:The accumulation and degradation in the endoplasmic reticulum (ER) of a truncated ER-60 protease, from which the C-terminal 89 amino acid residues have been deleted (K 417 ochre), was examined. K 417 ochre overexpressed in COS-1 cells is not secreted into the medium, but accumulates as insoluble aggregates in non-ionic detergent without degradation in unusual clump membrane structures. K 417 ochre, stably expressed, forms soluble aggregates in non-ionic detergent and is distributed in the reticular structures of ER. Under these conditions, K 417 ochre is not secreted into the medium but is degraded with a half-life time of more than 8 h. Since K 417 ochre/C all S, in which all the Cys residues of K 417 ochre are replaced by Ser, also forms aggregates, an inter-disulfide bond appears unnecessary for aggregation. In both types of aggregates, Ig heavy chain binding protein, calnexin, glucose regulated protein 94, calreticulin, ERp72, and protein disulfide isomerase are scarcely found. Since degradation of the stably expressed K 417 ochre was not inhibited by lactacystin, leupeptin, NH 4 Cl, or cytocharasin B, but was inhibited by N-acetyl-leucyl-leucyl-norleucinal, the self-aggregated abnormal protein in the lumen of ER is assumed to be degraded by an unknown protease system other than proteasome, lysosome or autophagy.
  • Catalytic cysteine residues of ER-60 protease, OKUDO H, URADE R, MORIYAMA T, KITO M, FEBS Letter, 465(2/3), 145 - 147, Jan. 14 2000
    Summary:ER-60 protease contains two CGHC motifs that appear to include an active site cysteine residue(s). Its proteolytic activity was lost with a double mutation of the C-terminal cysteines of the two motifs to alanine, but not with a single mutation of the C-terminal cysteine of either of the motifs to alanine. This suggests that these C-terminal cysteines independently constitute the catalytic active site. A mutation of both histidine residues in the two CGHC motifs to serine did not abolish the proteolytic activity, suggesting these histidine residues in the CGHC motifs do not constitute the catalytic dyad of ER-60 protease. Copyright (C) 2000 Federation of European Biochemical Societies.
  • Autodegradation of Protein Disulfide Isomerase., Reiko URADE, Ayako YASUNISHI, Hirokazu OKUDO, Tatsuya MORIYAMA, Makoto KITO, Bioscience, Biotechnology and Biochemistry, 63(3), 610 - 613, Jan. 01 1999
    Summary:Protein disulfide isomerase (PDI) and its degradation products were found in HepG2, COS-1, and CHO-K1 cells. Whether or not the products were formed through autodegradation of PDI was examined, since PDI contains the CGHC motif, which is the active center of proteolytic activity in ER-60 protease. Commercial bovine PDI was autodegraded to produce a trimmed PDI. In addition, human recombinant PDI also had autodegradation activity. Mutant recombinant PDIs with CGHC motifs of which cysteine residues were replaced with serine or alanine residues were prepared. However, they were not autodegraded, suggesting the cysteine residues of motifs are necessary for autodegradation. © 1999, Taylor & Francis Group, LLC. All rights reserved.
  • Purification and characterization of diacylglycerol lipase from human platelets, Tatsuya Moriyama, Reiko Urade, Makoto Kito, Journal of Biochemistry, 125, 1077 - 1085, Jan. 01 1999
    Summary:Diacylglycerol lipase (DGL) was solubilized from human platelet microsomes with heptyl-β-D-thioglucoside, and purified to homogeneity on SDS-PAGE using a combination of chromatographic and electrophoretic methods. The molecular mass of the purified DGL was estimated to be 33 kDa. Its apparent pI was pH 6.0, as determined by Immobiline isoelectrofocusing. The enzymatic activity of the partially purified DGL was investigated in the presence of a variety of inhibitors and reagents, as well as its pH and calcium dependence. Thiol reagents such as p-chloromercurubenzoic acid (pCMB), N-ethylmaleimide (NEM), and HgCl 2 , inhibited the activity, while dithiothreitol (DTT) and reduced glutathione (GSH) enhanced it. In addition, the enzymatic activity was inhibited by two serine blockers, phenylmethylsulfonyl fluoride (PMSF) and diisopropyl fluorophosphate (DFP), and by a histidine modifying reagent, p-bromophenacyl bromide (pBPB). These results suggest that cysteine, serine and histidine residues are required for the enzymatic activity of DGL. DGL was optimally active in the pH range of 7-8 and its activity did not change significantly in the presence of various calcium concentrations, even in the presence of 2 mM EGTA. This indicates that DGL can hydrolyze substrates with a basaI cytosolic free Ca 2+ level in the physiological pH range. A DGL inhibitor, RHC-80267, inhibited DGL activity in a dose-dependent manner with an IC 50 (the concentration required for 50% inhibition) of about 5 μM. Unexpectedly, several phospholipase A 2 (PLA 2 ) inhibitors were potent inhibitors of DGL activity (IC 50 , < 5 μM), suggesting that the catalytic mechanisms of DGL and PLA 2 may be similar. Finally, we show that DGL activity was inhibited by 2-monoacylglycerols (2-MGs), the reaction products of this enzyme. Among the three 2-MGs tested (2-arachidonoyl glyceroI, 2-stearoyl glycerol, and 2-oleoyl glycerol), 2-arachidonoyl glycerol was the most potent inhibitor.
  • Degradation of HMG-CoA Reductase in Vitro. Cleavage in the membrane domain by a membrane-bound cysteine protease, MORIYAMA T, SATHER S K, MCGEE T P, SIMONI R D, Journal of Biological Chemistry, 273(34), 22037 - 22043, Aug. 21 1998
    Summary:We have recently shown that the endoplasmic reticulum (ER) membrane protein, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, is cleaved in isolated membrane fractions enriched for endoplasmic reticulum. Importantly, the cleavage rate is accelerated when the membranes are prepared from cells that have been pretreated with mevalonate or sterols, physiological regulators of the degradation process in vivo (Mc-Gee, T. P., Cheng, H. H., Kumagai, H., Omura, S., and Simoni, R. D. (1996) J. Biol. Chem. 271, 25630-25638). In the current study, we further characterize this in vitro cleavage of HMG-CoA reductase. E64, a specific inhibitor of cysteine- proteases, inhibits HMG-CoA reductase cleavage in vitro. In contrast, lactacystin, an inhibitor of the proteasome, inhibits HMG-CoA reductase degradation in vivo but does not inhibit the in vitro cleavage. Purified ER fractions contain lactacystin-sensitive and E64-insensitive proteasome activity as measured by succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin hydrolysis. We removed the proteasome from purified ER fractions by solubilization with heptylthioglucoside and observed that the detergent extracted, proteasome-depleted membrane fractions retain regulated cleavage of HMG-CoA reductase. This indicates that ER-associated proteasome is not involved in degradation of HMG-CoA reductase in vitro. In order to determine the site(s) of proteolysis of HMG-CoA reductase in vitro, four antisera were prepared against peptide sequences representing various domains of HMG-CoA reductase and used for detection of proteolytic intermediates. The sizes and antibody reactivity of the intermediates suggest that HMG-CoA reductase is cleaved in the in vitro degradation system near the span 8 membrane region, which links the N-terminal membrane domain to the C-terminal catalytic domain of the protein. We conclude that HMG-CoA reductase can be cleaved in the membrane-span 8 region by a cysteine protease(s) tightly associated with ER membranes.
  • Functions of Characteristic Cys-Gly-His-Cys(CGHC)and Gln-Glu-Asp-Leu(QEDL)Motifs of Microsomal ER-60 Protease, Reiko Urade, Tsuneyuki Oda, Hiroshi Ito, Tatsuya Moriyama, Shigeru Utsumi, Makoto Kito, Journal of Biochemistry, 122, 834 - 842, Jan. 01 1997
    Summary:The human ER-60 protease cDNA was expressed in Escherichia coli BL21 (DE3) cells using the pET-20b (+) T7 promoter. The recombinant ER-60 protease was obtained in a water-soluble form and purified through four sequential chromatographies. The ER-60 protease contains two CGHC motifs. When an alanine residue was substituted for the N-terminal cysteine residue in both motifs, the protease activity was not lost. However, when the C-terminal cysteine residue in both motifs was replaced by a serine residue, the cysteine protease activity, which was inhibited by p-chloromercuribenzoic acid (pCMB) but not by diisopropyl fluorophosphate (DFP), changed to serine protease activity, which was inhibited by DFP but not by pCMB. These results indicate that the C-terminal cysteine resldue(s) of the CGHC motifs may constitute the active site(s) of ER-60 protease. The ER-60 protease has a C-terminal QEDL sequence, which was proved to serve as an ER-retention signal by deletion of the QEDL sequence. However, because QEDL could not serve as the ER-retention signal for protein disulfide isomerase or ERp72, it is suggested that amino acid residue(s) of ER-60 protease, other than the QEDL sequence itself, is complimentarily responsible for the ER retention of this protein.
  • The mechanism of arachidonic acid release in collagen-activated human platelets, Tatsuya Moriyama, Kazuteru Wada, Manabu Oki, Manabu Oki, Takao Matsuura, Takao Matsuura, Makoto Kito, Bioscience, Biotechnology and Biochemistry, 58, 93 - 98, Jan. 01 1994
    Summary:The mechanism of arachidonic acid (AA) release in collagen-activated human platelets was studied. An arachidonic acid metabolite, thromboxane B 2 (TXB 2 ), was formed in parallel with the formation of phosphatidic acid (PA) without formation of lysophosphatidic acid (lysoPA) or lysophosphatidylinositol (lysoPI) in the absence of extracellular Ca 2+ , suggesting that AA was released from PI via a Pi-specific phospholipase C (PI-PLC)/diacylglycerol (DG) lipase/monoacylglycerol (MG) lipase pathway under the cytosolic low Ca 2+ concentrations. Moreover, solubilized DG lipase and MG lipase could hydrolyze the substrates at basal cytosolic free Ca 2+ concentrations. Subsequently, the relationship of cytosolic free Ca 2+ concentrations and formation of AA metabolites was analyzed using Ca 2+ ionophore, A23187. Collagen was able to induce a release of small amounts of AA under basal cytosolic Ca 2+ conditions. However, a release of large amounts of AA was induced by phospholipase A 2 activated by both collagen-receptor occupancy and elevated Ca 2+ levels. A TXA 2 mimetic agonist, STA 2 induced all the responses except for AA release. From these results, the mechanism of AA release and signal transduction in collagen-activated human platelets is discussed. © 1994 Taylor & Francis Group LLC.
  • Changes in Plasma Lipid Levels in Young Healthy Volunteers by Adding an Extruder-cooked Soy Protein to Conventional Meals., Makoto Kito, Tatsuya Moriyama, Yuuko Kimura, Hirofumi Kambara, Bioscience, Biotechnology and Biochemistry, 57(2), 354 - 355, Jan. 01 1993
  • Protein Degradation by the Phosphoinositide-specific Phospholipase C-α Family from Rat Liver Endoplasmic Reticulum, R. Urade, M. Nasu, T. Moriyama, K. Wada, M. Kito, Journal of Biological Chemistry, 267(21), 15152 - 15159, Jan. 01 1992
    Summary:A 60-kDa protein homologous to phosphoinositide-specific phospholipase C- α was purified to apparent homogeneity on sodium dodecyl sulfate- polyacrylamide gel electrophoresis from the rough endoplasmic reticulum of rat liver through three sequential chromatographies on DEAE Toyopearl 650, AF-heparin Toyopearl 650M, and TSK gel G3000SW. The purified protein was monomeric, with an M(r) of 60,000. Eight types of protein were further separated from the 60-kDa protein and named ER60A-ER60H according to the order of their elution from a TSK gel DEAE-5PW column. They were essentially identical in terms of immunochemical properties and the NH 2 -terminal amino acid sequence. The partial amino acid sequence of ER60F showed homology to that of phosphoinositide-specific phospholipase C-α. ER60A-ER60H showed no phosphoinositide-specific phospholipase C activity. However, ER60A-ER60H catalyzed cleavage of themselves and the endoplasmic reticulum proteins protein disulfide-isomerase and calreticulin. Proteolytic degradation was inhibited by p-chloromercuribenzoate. These results indicate that ER60A-ER60H comprise a group of endoplasmic reticulum resident proteins and show thiol group-related proteolytic activity.
  • Purification of Polymeric Phospholipase Cs from Human Platelets, Tatsuya Moriyama, Hiroshi Narita, Manabu Oki, Takao Matsuura, Makoto Kito, Journal of Biochemistry, 108(3), 414 - 419, Sep. 01 1990
    Summary:Two types of cytosolic phospholipase C specific for phosphoinositides were purified from human platelets. The molecular masses of the purified enzymes were 440 and 290 kDa. These enzymes were concluded to be respectively a trimer and a dimer of homologous 146 kDa polypeptides. The 146 kDa polypeptide may be an immunologically novel isozyme among the 140-150 kDa PLC isozymes. Both enzymes hydrolyzed phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate in a Ca 2+ -dependent manner. © 1990 Copyright, 1990 by the Journal of Biochemistry.
  • Dihydropyridine-Sensitive and Insensitive Ca2+ Channels in Human Platelets, Tatsuya Moriyama, Hitoshi Takamura, Hiroshi Narita, Ken ichi Tanaka, Takao Matsuura, Makoto Kito, Journal of Biochemistry, 104(6), 875 - 877, Dec. 01 1988
    Summary:The Ca 2+ channel blocker, nifedipine, a dihydropyridine derivative, inhibited the Ca 2+ influx and release from internal stores caused by collagen or a low concentration of the thromboxane A 2 (TXA 2 analogue, 9,11-epithio-11,12-methano-TXA 2 (STA 2 (10 nM), but did not inhibit those caused by thrombin or a high concentration of STA 2 (100 nM). These results indicate the presence of two distinct, dihydropyridine-sensitive and insensitive, Ca 2+ channels dependent on the concentrations and classes of agonists in human platelets. © 1988 COPYRIGHT 1988 BY The Journal of Biochemistry.
  • Elevation of cytosolic free Ca2+is directly evoked by thromboxane A2in human platelets during activation with collagen, Tatsuya Moriyama, Hitoshi Takamura, Hiroshi Narita, Ken ichi Tanaka, Takao Matsuura, Makoto Kito, Journal of Biochemistry, 103, 901 - 902, Jun. 01 1988
    Summary:The thromboxane A 2 antagonist, ONO-3708, completely inhibited the increase in cytosolic free Ca 2+ in human platelets during activation with collagen. Half-maximal Ca 2+ release and influx required about 3 and 4 nM STA 2 , a stable thromboxane A 2 mimetic, respectively. However, half maximal activation of phospholipase C required about 18 nM STA 2 . This suggests that thromboxane A 2 directly causes Ca 2+ mobilization without further activation of phospholipase C during activation of human platelets with collagen. © 1988 BY The Journal of Biochemistry.

Works

  • Food factors regulating human lipid metabolism

Misc

  • 大豆アレルゲンに関する最近の知見, 福住綾乃, 松尾綾人, 矢野えりか, 財満信宏, 森山達哉, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 529, 586‐589,   2019 07 20 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902269616942071
  • 鶏卵との交叉反応性を認めなかったウズラの卵によるアレルギーの1例, 原田晋, 森山達哉, 上塚弘, 皮膚科の臨床, 61, 8, 1257‐1262,   2019 07 01 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902218564461191
  • 果実等の農作物に含まれる食品成分の健康機能性とアレルゲン性, 森山達哉, 和歌山医学, 70, 2, 92,   2019 06 30 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902254385970388
  • 循環不全が生じた腹部大動脈瘤壁に観察される脂肪細胞の異常出現とその出現機構, 財満信宏, 財満信宏, 久後裕菜, 森山達哉, 森山達哉, 脂質生化学研究, 61, 214,   2019 06 20 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902274331765957
  • マウスモデル系による牛乳タンパク質の経皮感作抗原の探索, 日高翔太, 高宮隆樹, 小川昂志, 高蓋秋穂, 矢野えりか, 財満信宏, 財満信宏, 森山達哉, 森山達哉, 月刊アレルギーの臨床, 527, 401‐404,   2019 05 20 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902234592086393
  • 農業関連喘息の原因物質と考えられる新規野菜抗原の同定, 関谷怜奈, 永野達也, 森山達哉, 波多野直哉, 梅澤佳乃子, 桂田直子, 山本正嗣, 中田恭介, 上領博, 小林和幸, 日高翔太, 矢野えりか, 篠原正和, 小谷義一, 福永淳, 西村善博, アレルギー, 68, 4/5, 499,   2019 05 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902289067320332
  • アレルゲン・抗原 農業関連喘息の原因物質と考えられる新規野菜抗原の同定, 関谷 怜奈, 永野 達也, 森山 達哉, 波多野 直哉, 梅澤 佳乃子, 桂田 直子, 山本 正嗣, 中田 恭介, 上領 博, 小林 和幸, 日高 翔太, 矢野 えりか, 篠原 正和, 小谷 義一, 福永 淳, 西村 善博, アレルギー, 68, 4-5, 499, 499,   2019 05
  • 成人における食物アレルギーの多様性:花粉症と経皮感作の関与, 日高翔太, 高葢秋穂, 小川昂志, 矢野えりか, 財満信宏, 財満信宏, 森山達哉, 森山達哉, 地域ケアリング, 21, 4, 89‐92,   2019 04 12 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902284616761393
  • 福祉の現場から 成人における食物アレルギーの多様性 花粉症と経皮感作の関与, 日高 翔太, 高葢 秋穂, 小川 昂志, 矢野 えりか, 財満 信宏, 森山 達哉, 地域ケアリング, 21, 4, 89, 92,   2019 04
    Summary:近年、乳幼児だけでなく、成人での食物アレルギーも増加している。その要因として、食物アレルギーの引き金となる、IgE抗体を産生させる感作経路が多様化していることが関与している。このような成人における食物アレルギーの発症は、介護と食の関連における新たな課題となる。そこで本稿では、成人における食物アレルギーの感作経路の多様化の原因として、花粉症に関連する食物アレルギーおよび、近年注目されている経皮感作について紹介する。(著者抄録)
  • 低栄養状態がマウス由来前駆脂肪細胞の脂肪蓄積とMMPs発現に及ぼす影響, 岩本和子, 吸原慧紀, 財満信宏, 財満信宏, 森山達哉, 森山達哉, 日本農芸化学会大会講演要旨集(Web), 2019, ROMBUNNO.4B1a08 (WEB ONLY),   2019 03 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902232729589734
  • 脂肪細胞機能を調節しうる農作物由来食品成分に関する研究, 家田彩菜, 高田祐一, 岩本和子, 財満信宏, 財満信宏, 森山達哉, 森山達哉, アグリバイオ, 3, 1, 47‐51,   2019 01 20 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902228088602698
  • 蕁麻疹・痒疹 大豆プロテイン飲料摂取後にアナフィラキシー症状を発症したクラス2大豆アレルギーの男性例, 原田晋, 森山達哉, 田中裕, 皮膚科の臨床, 60, 13, 1969‐1974,   2018 12 01 , 10.18888/hi.0000001108, https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802217528004531
  • 研究者の最新動向 生活習慣病を予防改善しうる食品成分に関する研究, 家田 彩菜, 高田 祐一, 岩本 和子, 財満 信宏, 森山 達哉, Precision Medicine, 1, 2, 215, 218,   2018 11
    Summary:食品に含まれる様々な成分には、生理機能性を有するものがある。エラグ酸は、ザクロ、ラズベリー、イチゴなどの様々な植物に含まれる天然のポリフェノールの1つである。また、大豆サポニンは大豆に含まれるトリテルペン配糖体である。私たちは、これらのエラグ酸や大豆サポニンが脂質代謝改善やアディポサイトカイン分泌の調節などによってメタボリック症候群を改善しうる可能性を明らかにした。これらの食品成分は、機能性食品素材として応用できる可能性がある。(著者抄録)
  • マツタケによるアナフィラキシーの1例, 爲定 由佳理, 李 和純, 西谷 奈生, 森山 達哉, 皮膚の科学, 17, 5, 284, 284,   2018 10
  • 他のキノコ類との交叉反応を認めなかったマツタケによるアナフィラキシーの1例, 原田 晋, 森山 達哉, 中村 順子, 皮膚科の臨床, 60, 7, 1085, 1089,   2018 06 , 10.18888/hi.0000000823
    Summary:<文献概要>23歳,女性。マツタケを含む食品摂取中にアナフィラキシー症状を発現した。プリックテストではマツタケ傘・マツタケ柄・加熱マツタケ傘・加熱マツタケ柄のすべてで(3+)陽性であり,マツタケ以外のキノコ類ではいずれも陰性であった。以上より,マツタケアレルギーと診断した。既報告では,加熱処理によって抗原性が減弱しうるとの報告や他のキノコ類でもアレルギー症状を呈する場合があるとの報告もあるが,自験例では (1) 加熱によるマツタケの抗原性の減弱は認められず,(2) 他のキノコ類との交叉反応性は有していないと考えた。またマツタケアレルギーの原因抗原に関して,immunoblotの結果からは25kDa付近のバンドが疑われた。
  • ニンジンとタマネギによるアナフィラキシーの1例, 加納 宏行, 服部 有希, 周 円, 森山 達哉, 清島 真理子, アレルギー, 67, 4-5, 645, 645,   2018 05
  • ネギによる食物依存性運動誘発アナフィラキシーの1例, 高松 法子, 平松 功太郎, 松本 桂, 鈴木 麻生, 伊藤 亜希子, 蒲原 毅, 森山 達哉, アレルギー, 67, 4-5, 648, 648,   2018 05
  • 鶏卵との交差反応性を認めなかったウズラの卵によるアレルギーの1例, 原田 晋, 森山 達哉, 上塚 弘, アレルギー, 67, 4-5, 666, 666,   2018 05
  • Gly m4が主要アレルゲンと考えられた,ソイプロテイン飲料初回摂取後のアナフィラキシーの1例, 浜岡 大, 大塚 晴彦, 山野 希, 井上 友介, 足立 厚子, 森山 達哉, 日本皮膚科学会雑誌, 128, 5, 1185, 1185,   2018 05
  • 「アレルギーの臨床」に寄せる 経皮感作とアレルゲン, 小川 昂志, 高葢 秋穂, 村上 浩規, 岩本 和子, 矢野 えりか, 財満 信宏, 森山 達哉, アレルギーの臨床, 38, 5, 472, 475,   2018 05
    Summary:食物アレルゲンの新たな感作経路として、皮膚を介した経皮感作が注目されている。現在明らかにされている経皮感作のメカニズムとしては、ランゲルハンス細胞や上皮細胞由来のサイトカインであるTSLPやIL-33などが主に作用して引き起こされることが明らかになってきた。経皮感作の関与が疑われる症例報告も含め、経皮感作に関する研究は増加している。そこで本稿では、総説として経皮感作の研究例やメカニズムについて簡単にまとめた。また、どのような抗原が経皮感作されるかという点についても、いくつかの報告を紹介する。(著者抄録)
  • 「花粉‐食物アレルギー症候群の病態と治療」に寄せる 各種食用豆類における花粉症関連食物アレルゲンの検出, 松尾綾人, 徳舛直樹, 矢野えりか, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 38, 511, 264‐267, 267,   2018 03 20 , http://jglobal.jst.go.jp/public/201802256491624346
    Summary:花粉症に関連する大豆アレルギー(大豆によるクラス2食物アレルギー)の主な原因抗原としては、Gly m4の他にGly m3が同定されている。このGly m4、Gly m3によって引き起こされる大豆のクラス2食物アレルギー患者が、大豆以外の豆類の摂取で同様の症状を交差反応によって発症するかどうかは不明である。そこで今回は、大腸菌にて発現させた完全長Gly m4、Gly m3に対して作製した抗体を用いて、各種食用豆類中にこれらの類似抗原が存在するかどうか、ウエスタンブロッティングによって検討した。(著者抄録)
  • ゴマ由来セサミン・セサモリンの水中油型乳剤の粒子径が脳内移行に及ぼす影響, 松村 晋一, 岩本 和子, 久後 裕菜, 橋本 佳祐, 宮本 智絵, 財満 信宏, 森山 達哉, 吉岡 百合, 日本薬学会年会要旨集, 138年会, 2, 211, 211,   2018 03
  • 「アレルギーの臨床」に寄せる―905―マウスモデル系を用いた卵白の経皮感作, 小川昂志, 高葢秋穂, 村上浩規, 岩本和子, 矢野えりか, 財満信宏, 森山達哉, 月刊アレルギーの臨床, 508, 1389‐1393,   2017 12 30 , http://jglobal.jst.go.jp/public/201702231579587052
  • 【食物アレルギー】 食物アレルギーの現状と多様性, 村上 浩規, 財満 信宏, 森山 達哉, 栄養, 2, 4, 203, 213,   2017 12
    Summary:食物アレルギー(FA)の定義、症状の多様性、感作源や感作経路の多様性などを中心に、下記について概説した。1)FAの定義と臨床型分類・多様性、2)発症機序によるFAの分類、3)花粉症に関連するFA(大豆アレルギーを例に)、4)食品が関係する特殊なFA、5)健康食品やサプリメントによるFAの可能性、6)年齢別で見たFA原因食品、7)経皮感作、8)経皮感作の機序、9)経口免疫寛容、10)経口免疫寛容の機序、11)FAの診断と対策、について述べた。
  • 「アレルギーの臨床」に寄せる マウスモデル系を用いた卵白の経皮感作, 小川 昂志, 高葢 秋穂, 村上 浩規, 岩本 和子, 矢野 えりか, 財満 信宏, 森山 達哉, アレルギーの臨床, 37, 14, 1389, 1393,   2017 12
    Summary:経皮的に抗原が侵入し、アレルギーを引き起こす「経皮感作」が近年注目されている。鶏卵の卵白に含まれるOVA(オボアルブミン)を用いた経皮感作実験はいくつか報告されているが、卵白中のOVA以外のタンパク質が経皮感作されるかどうかは不明である。そこで本研究では、卵白をマウス皮膚に塗布し、どのようなタンパク質が経皮感作しうるか、また、共存する界面活性剤の濃度によって感作の度合いが変化するかどうか検討した。その結果、塗布サンプルのSDS濃度が0.5%よりも5%の方がより早くIgE抗体が産生され、OVAと思われる抗原を含め複数のタンパク質が経皮感作しうることが明らかになった。(著者抄録)
  • 【食物アレルギー】 食物アレルギーの現状と多様性, 村上 浩規, 財満 信宏, 森山 達哉, 栄養, 2, 4, 203, 213,   2017 12
    Summary:食物アレルギー(FA)の定義、症状の多様性、感作源や感作経路の多様性などを中心に、下記について概説した。1)FAの定義と臨床型分類・多様性、2)発症機序によるFAの分類、3)花粉症に関連するFA(大豆アレルギーを例に)、4)食品が関係する特殊なFA、5)健康食品やサプリメントによるFAの可能性、6)年齢別で見たFA原因食品、7)経皮感作、8)経皮感作の機序、9)経口免疫寛容、10)経口免疫寛容の機序、11)FAの診断と対策、について述べた。
  • ピーナッツアレルギー,クルミアレルギーとの交差反応の可能性も疑われたゴマによるアナフィラキシーの1例, 原田晋, 森山達哉, 太田國隆, Journal of Environmental Dermatology and Cutaneous Allergology, 11, 5, 464, 464,   2017 11 06 , http://jglobal.jst.go.jp/public/201702288989634692
  • 大豆プロテイン飲料摂取後にアナフィラキシー症状を発症したクラス2大豆アレルギーの男性例, 原田晋, 森山達哉, 田中裕, Journal of Environmental Dermatology and Cutaneous Allergology, 11, 5, 464, 464,   2017 11 06 , http://jglobal.jst.go.jp/public/201702267572933812
  • ニンジンとタマネギによるアナフィラキシーの1例, 服部有希, 周円, 加納宏行, 森山達哉, 清島真理子, Journal of Environmental Dermatology and Cutaneous Allergology, 11, 5, 465, 465,   2017 11 06 , http://jglobal.jst.go.jp/public/201702298095236562
  • 乳酸発酵と酵素消化によるアレルゲン低減化大豆食品の開発と評価, 植田啄也, 矢野えりか, 立垣愛郎, 財満信宏, 森山達哉, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 56th, 29,   2017 11 01 , http://jglobal.jst.go.jp/public/201702287933875135
  • 花粉症関連大豆アレルゲンGly m4のELISA定量系の構築と検証, 花房佳世, 村上浩規, 岩本和子, 矢野えりか, 財満信宏, 森山達哉, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 56th, 29,   2017 11 01 , http://jglobal.jst.go.jp/public/201702258975112492
  • マウスモデル系を用いた卵白中の経皮感作抗原の同定と反応性比較, 小川昂志, 高葢秋穂, 村上浩規, 岩本和子, 矢野えりか, 財満信宏, 森山達哉, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 56th, 50,   2017 11 01 , http://jglobal.jst.go.jp/public/201702256257049047
  • 高シュークロース食の摂取が腹部大動脈瘤の進展・破裂に及ぼす影響の病理学的評価, 宮本智絵, 橋本佳祐, 久後裕菜, 財満信宏, 森山達哉, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 56th, 49,   2017 11 01 , http://jglobal.jst.go.jp/public/201702248981200109
  • 魚油摂取がニコチン投与マウスの血管壁に及ぼす影響の評価, 星野健斗, 久後裕菜, 小野里恵, 宮本智絵, 橋本佳祐, 柳本賢一, 財満信宏, 森山達哉, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 56th, 42,   2017 11 01 , http://jglobal.jst.go.jp/public/201702244716416846
  • CASE REPORTS : Anaphylaxis Caused by the Oral Intake of Curcuma aromatica, 原田 晋, 森山 達哉, 谷口 雅輝, 森岡 芳雄, 皮膚科の臨床, 59, 8, 1283, 1288,   2017 07 , http://ci.nii.ac.jp/naid/40021263626
  • 春ウコンの経口摂取により発症したアナフィラキシーの1例, 原田 晋, 森山 達哉, 谷口 雅輝, 森岡 芳雄, 皮膚科の臨床, 59, 8, 1283, 1288,   2017 07 , 10.18888/hi.0000000123
    Summary:64歳女性。健康食品として春ウコン粉末を初めて服用した直後より、アナフィラキシー症状が出現した。近医でステロイド剤点滴および内服による加療後、原因検索目的で当科へ紹介となった。臨床経過およびプリックテストの結果から、本症例を春ウコンによるアレルギーと診断した。反面、ターメリック、クルクミン、秋ウコンの健康ドリンクのプリックテストは陰性であったことから、秋ウコンに対する感作は生じていないと考えられた。また、花粉類に対するアレルギーの既往もなく、花粉類、果物類、サバの特異的IgEおよび果物類のプリックテストはすべて陰性であったため、これらの因子に対する感作は否定的と考えられた。
  • 腹部大動脈瘤の破裂機構の解明と破裂予防法の検討, 財満 信宏, 久後 裕菜, 田中 宏樹, 柳本 賢一, 佐々木 健, 浦野 哲盟, 海野 直樹, 森山 達哉, 脂質生化学研究, 59, 43, 43,   2017 06
  • 腹部大動脈瘤の破裂機構の解明と破裂予防法の検討, 財満 信宏, 久後 裕菜, 田中 宏樹, 柳本 賢一, 佐々木 健, 浦野 哲盟, 海野 直樹, 森山 達哉, 脂質生化学研究, 59, 43, 43,   2017 06
  • ヤーコンによるアナフィラキシーの1例, 伊藤 路子, 藤本 和久, 新井 悠江, 佐伯 秀久, 森山 達哉, アレルギー, 66, 4-5, 681, 681,   2017 05
  • 食物アレルギー アナフィラキシー、交差反応 他のキノコ類との交差反応を認めなかったマツタケによるアナフィラキシーの1例, 原田 晋, 森山 達哉, 中村 順子, アレルギー, 66, 4-5, 597, 597,   2017 05
  • Peanut Allergy Sensitized to Both Class 1 Allergen and Class 2 Allergen, 原田 晋, 森山 達哉, 皮膚科の臨床, 59, 2, 203, 207,   2017 02 , 10.18888/J01266.2017215726, http://ci.nii.ac.jp/naid/40021082164
  • CASE REPORTS : Anaphylaxis Caused by the Ingestion of Fig with Indetermined Cross-reactive Antigen, 原田 晋, 森山 達哉, 谷口 万紀子, 皮膚科の臨床, 58, 7, 1147, 1151,   2016 06 , 10.18888/J01266.2016305037, http://ci.nii.ac.jp/naid/40020879929
  • Mass Spectrometry Imaging for Visualization of Lipids, 久後 裕菜, 山本 彩実, 森山 達哉, 財満 信宏, オレオサイエンス, 16, 5, 225, 231,   2016 05 , http://ci.nii.ac.jp/naid/40020838118
  • Applications of MALDI-IMS for Food Science, KUGO Hirona, YAMAMOTO Ayami, MORIYAMA Tatsuya, ZAIMA Nobuhiro, Journal of the Mass Spectrometry Society of Japan, 64, 1, 11, 15,   2016 , 10.5702/massspec.S16-03, http://ci.nii.ac.jp/naid/130005125541
  • アントシアニンの体内動態解析, 財満 信宏, 稲葉 栞里, 藤森 隆道, 荘厳 哲哉, 小林 沙織, 森山 達哉, JSBMS Letters, 40, Suppl., 98, 98,   2015 08
  • アントシアニンの体内動態解析, 財満 信宏, 稲葉 栞里, 藤森 隆道, 荘厳 哲哉, 小林 沙織, 森山 達哉, JSBMS Letters, 40, Suppl., 98, 98,   2015 08
  • Three Cases of Oral Allergy Syndrome Due to Walnut : Detection of IgE-Binding Walnut Proteins, 飯島 茂子, 角田 孝彦, 森山 達哉, Journal of environmental dermatology and cutaneous allergology, 8, 2, 95, 102,   2014 04 , http://ci.nii.ac.jp/naid/40020095827
  • The allergen risk of various food materials, 森山 達哉, 食品の包装 = Packaging of foodstuff, 46, 1, 32, 38,   2014 , http://ci.nii.ac.jp/naid/40020192842
  • CASE REPORTS : Immediate-type Allergy Due to a Variety of Vegetables after Percutaneous Occupational Sensitization of Henna, 舟木 聡子, 加藤 敦子, 森山 達哉, 皮膚科の臨床, 55, 11, 1401, 1404,   2013 11 , http://ci.nii.ac.jp/naid/40019873093
  • Pollen-food Allergy Syndrome Causing Anaphylactic Symptoms after the Intake of Beansprout, 原田 晋, 森山 達哉, 有津 崇, 皮膚科の臨床, 55, 8, 1013, 1017,   2013 08 , http://ci.nii.ac.jp/naid/40019786108
  • Evaluation of Hypoallergenicity and Health-Promoting Effect of Miso, 森山 達哉, 中央味噌研究所研究報告, 34, 75, 80,   2013 03 , http://ci.nii.ac.jp/naid/40019727895
  • Study on physiological function of soyasaponins, 森山 達哉, 杉山産業化学研究所年報, 64, 82,   2013 , http://ci.nii.ac.jp/naid/40020258781
  • A CASE OF AN ALLERGIC REACTION DUE TO ANISAKIS SIMPLEX POSSIBLY AFTER THE INGESTION OF SQUID : SUCCESSFUL DETECTION OF FOUR A. SIMPLEX ALLERGENS, Ani s 1, Ani s 2, Ani s 12 AND TROPONIN C-LIKE PROTEIN, Iijima Shigeruko, Moriyama Tatsuya, Ichikawa Hidetaka, Kobayashi Yukihiro, Shiomi Kazuo, Japanese Journal of Allergology, 61, 8, 1104, 1110,   2012 08 30 , 10.15036/arerugi.61.1104, http://ci.nii.ac.jp/naid/110009495642
    Summary:A 62-year-old man ingested dressed salmon and its roe (ikura) and grilled mackerel and one hour later further ingested raw tuna and squid as an evening meal at a bar. Soon after the ingestion of raw seafood, he showed wheals, loss of consciousness and low blood pressure. Specific serum IgE to the nematode Anisakis simplex was positive but those to some seafoods were negative. Moreover, a skin prick test using the crude extract was positive for A. simplex but negative for the seafoods, which he ingested on the day of the above episode. When the A. simplex extract was analyzed by IgE-binding immunoblot analysis using the patient serum, two highly intense protein bands were recognized at 18 and 17kDa, one intense band at 35 kDa and two weak bands at 28 and 26 kDa. ELISA with 11 natural or recombinant A. simplex allergens (Ani s 1-6, 8, 9, 11 and 12 and troponin C-like protein) showed that the patient serum strongly reacted to Ani s 1 and Ani s 12 and weakly to Ani s 2 and troponin C-like protein. Based on these results, he was diagnosed as IgE-mediated A. simplex allergy due to four allergens (Ani s 1, Ani s 2, Ani s 12 and troponin C-like protein), possibly infested in the raw squid which he had ingested just before manifestation of allergic reactions.
  • Evaluation of Hypoallergenicity and Health-Promoting Effect of Miso, 森山 達哉, 中央味噌研究所研究報告, 33, 197, 201,   2012 03 , http://ci.nii.ac.jp/naid/40019238665
  • Novel allergy risk to recent food stuff, 森山 達哉, 日本食品新素材研究会誌 = Journal of Japanese Council for Advanced Food Ingredients Research, 15, 2, 51, 57,   2012 , http://ci.nii.ac.jp/naid/40020324787
  • A Case of Anaphylaxis due to Soy Milk, Caused by the Combination of Class 1 and Class 2 Food Allergy, IIJIMA Shigeruko, MORIYAMA Tatsuya, Journal of environmental dermatology and cutaneous allergology, 5, 5, 439, 449,   2011 10 31 , http://ci.nii.ac.jp/naid/10030440872
  • Diversity of Soybean Allergy and Evaluation of Hypoallergenicity of Miso, MORIYAMA Tatsuya, Journal of the Brewing Society of Japan, 106, 10, 645, 655,   2011 10 15 , 10.6013/jbrewsocjapan.106.645, http://ci.nii.ac.jp/naid/10029837536
    Summary: 近年,花粉症,アトピー性皮膚炎等のアレルギー症や食物アレルギーが増加し,その治療法のために原因究明の研究が精力的に行われている。中でも大豆アレルギー等の食物アレルギーについて抗原蛋白質が同定されるなど研究が進んでいる。近年の研究によってアレルギー発症機構からクラス1,クラス2の食物アレルギーが存在することがわかってきた。味噌,醤油,納豆等のわが国の伝統発酵食品と大豆アレルギーとの関連性が注目されているが,アレルゲン蛋白質の消長に関する研究が進んでいる。本解説では,食物アレルギー研究をご専門とする著者に,最新の研究成果の一端を紹介していただくとともに食物アレルギーの多様性と味噌のアレルゲン蛋白質の低減化について分かり易く解説していただいた。
  • TWO CASES OF ROYAL JELLY ALLERGY PROVOKED THE SYMPTOMS AT THE TIME OF THEIR FIRST INTAKE, Harada Susumu, Moriyama Tatsuya, Tanaka Akira, Japanese Journal of Allergology, 60, 6, 708, 713,   2011 06 30 , 10.15036/arerugi.60.708, http://ci.nii.ac.jp/naid/110008673093
    Summary:Two young women were suffered from several symptoms after the intake of royal jelly at their first time. According to the positive skin prick test reactions of raw royal jelly, royal jelly allergy was diagnosed. As the reasons why the symptoms appeared at the time of their first intake, we guessed the possibility that 1) they had been sensitized for royal jelly formerly, or 2) their symptoms were induced by the cross-reactivity between royal jelly and other allergens such as bee, honey and pollens. As to our cases, no related allergens were found in one case, but in another case co-existance of mugwort allergy was suspected from the results of both skin prick test and specific IgE titers. Originally royal jelly allergy has been regarded as class 1 allergic reaction developed by the sensitization of itself. But we speculated the possibility that there can also be cases of class 2 royal jelly allergy by the mechanism of cross-reaction with pollens.
  • Evaluation of hypoallergenicity and health-promoting effect of miso, 森山 達哉, Report of Central Miso Research Institute., 32, 59, 63,   2011 03 , http://ci.nii.ac.jp/naid/40018808113
  • Case reports: Lychee and pistachio allergy with suspected cross-reactivity with timothy, 原田 晋, 三好 麻里, 森山 達哉, 皮膚科の臨床, 53, 3, 421, 425,   2011 03 , http://ci.nii.ac.jp/naid/40018749624
  • A case whose symptom was provoked only by the intake of Oboro-Tofu or Yuba, 原田 晋, 森山 達哉, 田中 昭, 皮膚科の臨床, 52, 8, 1109, 1113,   2010 08 , http://ci.nii.ac.jp/naid/40017258364
  • Recent situation of food allergen in Japan and measures for patients: progress in research on soybean allergy, 小川 正, 森山 達哉, Journal of Urban Living and Health Association, 54, 2, 93, 105,   2010 , 10.11468/seikatsueisei.54.93, http://ci.nii.ac.jp/naid/40017118468
  • Recent Situation of Food Allergy in Japan and Measures for Patients, OGAWA Tadashi, MORIYAMA Tatsuya, SEIKATSU EISEI (Journal of Urban Living and Health Association), 54, 2, 93, 105,   2010 , 10.11468/seikatsueisei.54.93, http://ci.nii.ac.jp/naid/130004449000
    Summary:The increase in allergy patients in the developed countries, including Japan, has become a serious social problem. Of the many foods that elicit adverse reactions, soybean and soybean products, at the same time as representing an important protein source, are known as one of the major allergenic foodstuffs for the Japanese. However, no effective cure for allergic patients has been established. The strict elimination of the offending foods from the diet is therefore generally adopted as a prophylactic method in the treatment of food allergies, but may, if nutritional-fundamental foods are withdrawn for extended periods, lead to malnutrition in young patients. Accordingly, there is an urgent demand for food scientists to identify the proteins responsible for the allergic manifestation following ingestion of the foodstuff and to reduce allergenicity to enable the healthy use of soybean products. The present paper reviews recent information on the major allergens in soybean and the development of hypoallergenic soybean products. Further information on soybean allergens, sensitization to soybean allergens, persistence and symptoms of soybean allergy, and diagnostic features are available in a database at the Internet Symposium on Food Allergens: http://www.food-allergens.de.
  • Effects of Kale on Alcohol Metabolism : Clinical Test and Gene Expression Study, ISHII Yuri, MANJI Aiko, HAYAMIZU Kohsuke, OHNO Tomohiro, TSUKAMOTO Shojiro, ISOBE Eiji, FURUSE Mitsuhiro, MORIYAMA Tatsuya, Journal of Japanese Society of Clinical Nutrition, 30, 4, 321, 329,   2009 11 15 , http://ci.nii.ac.jp/naid/10026439259
  • ANAPHYLAXIS DUE TO PEACH WITH NEGATIVE ImmunoCAP RESULT TO PEACH ALLERGENS, INCLUDING rPru p 1, rPru p 3, AND rPru p 4 : A REPORT OF TWO CASES, Maeda Nobuko, Inomata Naoko, Morita Akiko, Kirino Mio, Moriyama Tatsuya, Ikezawa Zenro, Japanese Journal of Allergology, 58, 2, 140, 147,   2009 02 28 , 10.15036/arerugi.58.140, http://ci.nii.ac.jp/naid/110007161209
    Summary:We report two cases of anaphylactic reactions to peach with negative result of ImmunoCAP to peach. Case 1 is a 35-year-old man, who felt an itch in his oral cavity immediately after ingesting a whole fresh peach. He rapidly developed generalized urticaria, dyspnea, vomiting, and loss of consciousness. He recovered after treatment at a local hospital, thereafter he was referred to our hospital because ImmunoCAP conducted for screening allergens revealed a negative test result to peach and the cause of anaphylaxis remained unclear. He had a history of pollinosis. He reported that he previously felt an itch on his oral cavity after ingesting melon, watermelon, apple, and strawberry. Serum total IgE was 436IU/ml. CAP-RAST revealed negative results to peach, strawberry and kiwi. Skin prick tests (SPTs) with raw peach pulp, canned peach pulp, strawberry and kiwi were positive. Case 2 is a 30-year-old woman who felt an itch on her oral cavity accompanied by blepharedema, rhinorrhea, generalized urticaria, nausea, abdominal pain and diarrhea after eating peach. She had a history of pollinosis. She reported that she previously developed urticaria after ingesting an apple. Serum total IgE was 85IU/ml. ImmunoCAP revealed negative results to peach and apple. SPTs with canned yellow peach, strawberry and apple were positive. Consequently, the two patients were diagnosed with anaphylaxis due to peach, and allergic symptoms have never recurred since they avoided ingesting peach. Furthermore, in two patients ImmunoCAP to rPru p 1, rPru p 3, and rPru p 4 were negative. However, in IgE-immunoblotting of peach, serum IgE antibodies of two patients were bound to approximately 10kDa proteins. Meanwhile, the cross-reactivity between Rosaceae fruits, such as peach, apple, apricot, and plum, has been reported. These results suggest that in patients, who are suspected of having peach anaphylaxis and show a negative ImmunoCAP result to peach, the additional testing, such as SPT with peach, should be performed for diagnosis.
  • The Causative Antigen of Peach Allergy : Comparison Between the Patients with Anaphylaxis to Peach and Oral Allergy Syndrome to Many Fruits Including Peach, FUKUMOTO Takeshi, ADACHI Atsuko, UENO Mitsuhiko, NISITANI Nao, FUJIWARA Norihiro, SASAKI Yoshihito, USUKI Akiko, KUSAKABE Keigo, TANAKA Akira, MORIYAMA Tatsuya, Journal of environmental dermatology and cutaneous allergology, 3, 1, 23, 31,   2009 01 31 , http://ci.nii.ac.jp/naid/10024942898
  • C201 Increase of pathogenesis-related allergens in plants made by organic culture and prevention of its increase by pesticide treatment, Moriyama T., Nakajima K., Ohba M., Kitta K., Kawamoto S., Kominami Y., Kawada T., Ogawa T., Matsunaka K., Kawamura Y., 講演要旨集, 33, 33,   2008 03 11 , http://ci.nii.ac.jp/naid/110006858204
  • Two Cases of Immediate Hypersensitivity to Royal Jelly Examined by Immunoblotting with Their Sera, TAKAHASHI Toshiya, OKUNO Kousei, TSUNODA Takahiko, MORIYAMA Tatsuya, Japanese journal of dermatology, 118, 1, 43, 46,   2008 01 20 , 10.14924/dermatol.118.43, http://ci.nii.ac.jp/naid/10024430744
  • Two Cases of Immediate Hypersensitivity to Royal Jelly Examined by Immunoblotting with Their Sera, Takahashi Toshiya, Okuno Kousei, Tsunoda Takahiko, Moriyama Tatsuya, The Japanese Journal of Dermatology, 118, 1, 43, 46,   2008 , 10.14924/dermatol.118.43, http://ci.nii.ac.jp/naid/130004708555
    Summary:Royal jelly (RJ) is contained in health supplements, health food products, and cosmetics. We reported two cases of immediate hypersensitivity to RJ. In the skin prick test, a positive reaction was observed in response to two commercial RJ extracts in both cases. In immunoblotting, we detected bands at MW 47 kDa, 55 kDa, and 65–70 kDa. Among these proteins, no component of MW 65–70 kDa has previously been reported. From these findings, we suspect that this component may be a new and important allergen to RJ. However, this has not yet been proven by the skin prick test or the oral provocation test for the ethical problem.
  • TWO CASES OF APIACEAE SPICE ALLERGY, Harada Susumu, Matsunaga Akiko, Miyachi Rieko, Masaki Taro, Moriyama Tatsuya, Japanese Journal of Allergology, 56, 12, 1515, 1521,   2007 12 30 , 10.15036/arerugi.56.1515, http://ci.nii.ac.jp/naid/110006547232
    Summary:Background: Many cases of spice allergy have been reported especially from Scandinavian countries, but in contrast there are few reports in Japan. This time we experienced two cases of apiaceae spice allergy and practiced some kinds of examinations. We report here these two cases with the consideration concerning mechanizm of spice allergy. Methods and Subjects: We practiced 1) specific IgE of pollens and foods, 2) prick tests of spices and apiaceae vesitables, 3) immunoblot of spices, against two cases suspected spice allergy from their clinical courses. Clinically Case 1 32y.o. male had been no history of pollinosis, in contrast Case 2 46y.o. female had been suffered from pollinosis during spring and autumn seasons. Results: In Case 1 the scores of specific IgE of pollens were almost negative and immunoblot examination of spices revealed positive reaction at the site of 10〜12kDa and 60kDa. In Case 2 the scores of specific IgE of pollens were positive in many species and immunoblot examination of spices reacted positively at the site of 14kDa and 60kDa. Both of them showed positive reactions against many kinds of apiaceae spices in prick tests, so we diagnosed them as apiaceae spice allergy. Conclusion: According to these results we suspected Case 1 as class 1 allergy induced by the sensitization of spices themselvs and Case 2 as class 2 allergy caused by the cross reactions with pollinosis. So there may be some different mechanizms in the occurrence of spice allergy. In the future the occurrence of spice allergy will be supposed to increase and it will be necessary for us to pay much more attention to spice allergy even in Japan.
  • Oral Allergy Syndrome to Soybean Concomitant with Birch Pollen Allergy : Clinical Investigation and Analysis of Cross-Reactive Antigen, FUKUDA Kyoko, OCHIAI Toyoko, AIKAWA Miwa, TOGO Kana, TOMITA Yasuyuki, MORIYAMA Tatsuya, Journal of environmental dermatology and cutaneous allergology, 1, 2, 124, 130,   2007 07 31 , http://ci.nii.ac.jp/naid/10024293333
  • Three Cases of Soy Milk Allergy, HARADA Susumu, NAKAMURA Akiko, MATSUNAGA Akiko, IIJIMA Maya, YOSHIZAKI Yoshitsugu, SAITOH Kenji, ADACHI Atsuko, MORIYAMA Tatsuya, Journal of environmental dermatology and cutaneous allergology, 1, 1, 31, 38,   2007 04 30 , http://ci.nii.ac.jp/naid/10024293132
  • Studies on identification and reduction of class-2 food allergens in popular crops, 森山 達哉, Annual report, 2007, 43, 47,   2007 , http://ci.nii.ac.jp/naid/40016792110
  • Oral Allergy Syndrome with Anaphylaxis Due to Lychee and Various Kinds of Apiaceae Spices, ADACHI Atsuko, MORIYAMA Tatsuya, SHIMOURA Shinichi, SASAKI Yoshihito, SHIMIZU Hideki, IGUCHI Kayo, FUKUNAGA Atsushi, HORIKAWA Tatsuya, 日本皮膚科学会雑誌, 116, 13, 2212, 2217,   2006 12 15 , http://ci.nii.ac.jp/naid/10027062189
  • Two Cases of Oral Allergy Syndrome to "Fukinoto", KAGATANI Saori, TSUNODA Takahiko, MORIYAMA Tatsuya, 日本皮膚科学会雑誌 = THE JAPANESE JOURNAL OF DERMATOLOGY, 116, 3, 331, 334,   2006 03 20 , 10.14924/dermatol.116.331, http://ci.nii.ac.jp/naid/10018200759
  • Two Cases of Oral Allergy Syndrome to "Fukinoto", Kagatani Saori, Tsunoda Takahiko, Moriyama Tatsuya, The Japanese Journal of Dermatology, 116, 3, 331, 334,   2006 , 10.14924/dermatol.116.331, http://ci.nii.ac.jp/naid/130004708433
    Summary:"Fukinoto" is a wild vegetable and often eaten with all of its pollen as "tempura-style". We reported two cases of oral allergy syndrome to "Fukinoto". In the skin prick test, a positive reaction was observed for the pollen of "Fukinoto" in both cases, and, in Case 2, the reaction was also positive for heated pollen. In immunoblotting, we detected bands at 15 kDa and 20 kDa, possibly corresponding to pollen allergens. From these findings, we suspect that the pollen in "Fukinoto" contains an allergen and that the patients were sensitized directly through the oral mucosa.
  • Reduction of allergenic proteins in ripe tomato by the heterologous effect of the rin mutant gene, ITO Y., KITAGAWA M., MORIYAMA T., ITO H., ADACHI A., YASUDA J., INAKUMA T., ISHIGURO Y., KASUMI T., 園芸学会雑誌. 別冊, 園芸学会大会研究発表, 74, 2,   2005 10 01 , http://ci.nii.ac.jp/naid/10019589353
  • Food Composition Database for Safety Assessment of Genetically Modified Crops as Foods and Feeds, Kitta Kazumi, Ebihara Mitsuko, Hino Akihiro, Isshiki Kenji, Iizuka Tayoshi, Yoshikawa Reiji, Saito Kazuo, Ushio Fusao, Ogino Shuzo, Iguchi Masao, Shimamura Yasuhiro, Kanaya Kenichiro, Ogawa Tadashi, Moriyama Tatsuya, Kawamoto Shinichi, Japanese journal of food chemistry, 12, 1, 1, 9,   2005 04 29 , 10.18891/jjfcs.12.1_1, http://ci.nii.ac.jp/naid/110007367305
    Summary:Genetically modified (GM) crops have been increasingly entering into the market in recent years. The most common genetic modifications in crops confer an herbicide or an insect resistance to the plant, and some genetically modified plants anticipated with altered nutrient composition, functionality, and medicinal properties. In Japan, 58 GM crops have already been authorized and considered marketable. However, many concerns about incoming GM crops have been expressed by consumers, and the concerns are mainly focused on the safety assessment of GM crops intended for human consumption. While the most important factor taken into account in the safety assessment is the primary effect derived from newly introduced protein, possible unintended effects attributed to the insertion of defined DNA sequences have to be carefully examined in parallel. However, food is complex mixtures of compounds characterized by wide variation in composition and nutritional values. The food constituents are significantly affected by cultivars and environmental factors and thus very difficult to detect any potential adverse effects. A comparative approach focusing on the determination of differences between the GM food and its conventional counterpart will elicit potential safety issues and is considered the most appropriate strategy for the safety assessment of GM foods. For the efficient safety assessment of GM foods, accumulation of food composition data with wide variation under usual cultivation conditions and standardized validated methodologies in conventional foods is indispensable. To achieve this, we developed an internet-accessible food compound database comprising macro-, micro-nutrients, antinutrients, endogenous toxicants, and physiologically active substances of staple crops, such as rice and soybean.
  • Inter-laboratory Evaluation Studies for Establishment of Notified ELISA Methods for Allergic Substances (Peanuts), AKIYAMA Hiroshi, NAKAMURA Kenjin, HARIKAI Naoki, WATANABE Hiroko, IIJIMA Ken, YAMAKAWA Hirohito, MIZUGUCHI Yamato, YOSHIKAWA Reiji, YAMAMOTO Miho, SATO Hidetaka, WATAI Masatoshi, ARAKAWA Fumihiro, OGASAWARA Takeshi, NISHIHARA Rikuka, KATO Hisashi, YAMAUCHI Atsushi, TAKAHATA Yoshihisa, MORIMATSU Fumiki, MAMEGOSHI Shinichi, MURAOKA Shiroo, HONJOH Tsutomu, WATANABE Takahiro, SAKATA Kozue, IMAMURA Tomoaki, TOYODA Masatake, MATSUDA Rieko, MAITANI Tamio, Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 45, 6, 325, 331,   2004 12 25 , 10.3358/shokueishi.45.325, http://ci.nii.ac.jp/naid/10015435787
    Summary:Inter-laboratory evaluation studies were conducted for ELISA methods for allergic substances (peanuts). Extracts of biscuit, sauce, chocolate and butter spiked with peanut standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Peanut Protein ELISA Kit (Peanut kit) and a FASTKITTM Peanut ELISA kit (Peanut ELISA kit) were mostly below 10%. Mean recoveries of the peanut standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of peanut standard protein in four food extracts were in the ranges of 15.2-49.7% and 3.0-28.3% for the Peanut kit and the Peanut ELISA kit, respectively. Reproducibility relative standard deviations of peanut standard protein in four food extracts were 23.5-44.4%, 9.6-28.4% for the Peanut kit and the Peanut ELISA kit, respectively. The detection limits of both ELISA methods were 2-2.5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of peanut protein levels in extracts of biscuit, sauce, chocolate and butter.
  • Inter-laboratory Evaluation Studies for Establishment of Notified ELISA Methods for Allergic Substances (Buckwheat), AKIYAMA Hiroshi, NAKAMURA Kenjin, HARIKAI Naoki, WATANABE Hiroko, IIJIMA Ken, YAMAKAWA Hirohito, MIZUGUCHI Yamato, YOSHIKAWA Reiji, YAMAMOTO Miho, SATO Hidetaka, WATAI Masatoshi, ARAKAWA Fumihiro, OGASAWARA Takeshi, NISHIHARA Rikuka, KATO Hisashi, YAMAUCHI Atsushi, TAKAHATA Yoshihisa, MORIMATSU Fumiki, MAMEGOSHI Shinichi, MURAOKA Shiroo, HONJOH Tsutomu, WATANABE Takahiro, SAKATA Kozue, IMAMURA Tomoaki, TOYODA Masatake, MATSUDA Rieko, MAITANI Tamio, Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 45, 6, 313, 318,   2004 12 25 , 10.3358/shokueishi.45.313, http://ci.nii.ac.jp/naid/10015435757
    Summary:Inter-laboratory evaluation studies were conducted for the ELISA methods for allergic substances (buckwheat). Extracts of snack, bun and udon spiked with buckwheat standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate at 10 laboratories. Coefficients of variation (CVs) of the ELISA methods using a Buckwheat Protein ELISA Kit (Buckwheat kit) and a FASTKITTM Buckwheat ELISA kit (Buckwheat ELISA kit) were mostly below 10%. Mean recoveries of the buckwheat standard protein from the food extracts were over 40% in the two ELISA methods. Repeatability relative standard deviations of buckwheat standard protein in three food extracts were in the ranges of 6.8-78.5% and 5.0-33.9% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. Reproducibility relative standard deviations of buckwheat standard protein in three food extracts were 11.9-69.5% and 16.5-34.1% for the Buckwheat kit and the Buckwheat ELISA kit, respectively. The detection limits of both ELISA methods were 1 ng/mL in sample solutions. These results suggest that the notified ELISA methods are reliable and reproducible for the inspection of buckwheat protein levels in extracts of snack, bun and udon.
  • Inter-laboratory Evaluation Studies for Development of Notified ELISA Methods for Allergic Substances (Wheat), AKIYAMA Hiroshi, ISUZUGAWA Kazuto, HARIKAI Naoki, WATANABE Hiroko, IIJIMA Ken, YAMAKAWA Hirohito, MIZUGUCHI Yamato, YOSHIKAWA Reiji, YAMAMOTO Miho, SATO Hidetaka, WATAI Masatoshi, ARAKAWA Fumihiro, OGASAWARA Takeshi, NISHIHARA Rikuka, KATO Hisashi, YAMAUCHI Atsushi, TAKAHATA Yoshihisa, MORIMATSU Fumiki, MAMEGOSHI Shinichi, MURAOKA Shiroo, HONJOH Tsutomu, WATANABE Takahiro, SAKATA Kozue, IMAMURA Tomoaki, TOYODA Masatake, MATSUDA Rieko, MAITANI Tamio, Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 45, 3, 128, 134,   2004 06 25 , 10.3358/shokueishi.45.128, http://ci.nii.ac.jp/naid/10013655554
    Summary:Extracts of sausage, sauce, pasta sauce, fish paste and cereal spiked with wheat standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of both ELISA methods using a Wheat Protein ELISA Kit (Gliadin kit) and a FASTKITTM Wheat ELISA Kit (Wheat ELISA kit) were mostly below 10%. Mean recoveries of the wheat standard protein from the food extracts were over 40% in the two ELISA methods except those from cereal extract determined using the Wheat ELISA kit. Repeatability relative standard deviations of wheat standard protein in the five food extracts were in the ranges of 16-26.9% and 3.7-36.2% for the Gliadin kit and the Wheat ELISA kit, respectively. Reproducibility relative standard deviations of wheat standard protein in the five food extracts were 21.6-38.5%, 29.7-53.8% for the Gliadin kit and the Wheat ELISA kit, respectively. The recoveries of wheat standard protein from the cereal extract were improved by the increasing the amount of antibody coated on the plate in the Wheat ELISA kit. The detection limits of both ELISA methods were 1 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of wheat protein levels in extracts of sausage, sauce, pasta sauce, fish paste and cereal.
  • Inter-laboratory Evaluation Studies for Development of Notified ELISA Methods for Allergic Substances (Milk), AKIYAMA Hiroshi, ISUZUGAWA Kazuto, HARIKAI Naoki, WATANABE Hiroko, IIJIMA Ken, YAMAKAWA Hirohito, MIZUGUCHI Yamato, YOSHIKAWA Reiji, YAMAMOTO Miho, SATO Hidetaka, WATAI Masatoshi, ARAKAWA Fumihiro, OGASAWARA Takeshi, NISHIHARA Rikuka, KATO Hisashi, YAMAUCHI Atsushi, TAKAHATA Yoshihisa, MORIMATSU Fumiki, MAMEGOSHI Shinichi, MURAOKA Shiroo, HONJOH Tsutomu, WATANABE Takahiro, SAKATA Kozue, IMAMURA Tomoaki, TOYODA Masatake, MATSUDA Rieko, MAITANI Tamio, Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 45, 3, 120, 127,   2004 06 25 , 10.3358/shokueishi.45.120, http://ci.nii.ac.jp/naid/10013655537
    Summary:Extracts of sausage, sauce, cookie, cereal and pasta sauce spiked with milk standard protein at a level of 5-20 ng/mL as sample solutions were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of the three ELISA methods using a Milk Protein Casein ELISA Kit (Casein kit), a Milk Protein β-Lactoglobulin ELISA Kit (β-Lactoglobulin kit) and a FASTKITTM Milk ELISA Kit (Milk ELISA kit) were mostly below 10%. Mean recoveries of the milk standard protein from the food extracts were over 40% in the three ELISA methods with a few excertions. The recoveries of milk standard protein from the sauce extract in Casein kit were improved by adjusting the extract to neutrality before the Casein kit assay. The recoveries of milk standard protein from cookie, cereal and pasta sauce were improved by the increasing the amount of antibody coated in the Milk ELISA kit.
    The detection limits of all the ELISA methods were 1 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of milk protein levels in extracts of sausage, sauce, cookie, cereal and pasta sauce.
  • Fluorescence Detection of Lipids and Its Application, MORIYAMA Tatsuya, Oleoscience, 4, 4, 157, 166,   2004 04 01 , 10.5650/oleoscience.4.157, http://ci.nii.ac.jp/naid/10012704759
  • Fluorescence Detection of Lipids and Its Application, MORIYAMA Tatsuya, Journal of Oil Chemists' Society, Japan, 4, 4, 157, 166,146,   2004 , 10.5650/oleoscience.4.157, http://ci.nii.ac.jp/naid/130004650755
    Summary:Recently various fluorescent reagents have been developed for detecting or probing nucleotides, proteins, and other molecules. Highly sensitive detection devices have also been developed. One of the most popular devices is fluorescent scanner or fluorescent imager. These analyzers are useful for detection and quantification of lipids stained with various fluorescent dyes and fluorescent lipids. Neutral lipids or phospholipids were separated on TLC and stained with fluorescent dyes. Stained lipids could be detected and quantified by a laser-fluorescent scanner. In addition, various kinds of fluorescent lipids have been developed and used for various experiments. Thus, fluorescence detection of lipids is useful and applicable to various lipid studies in post-genome era.
  • Inter-laboratory Evaluation Studies of Notified ELISA Methods for Allergic Substances (Egg), AKIYAMA Hiroshi, ISUZUGAWA Kazuto, HARIKAI Naoki, WATANABE Hiroko, IIJIMA Ken, YAMAKAWA Hirohito, MIZUGUCHI Yamato, YOSHIKAWA Reiji, YAMAMOTO Miho, SATO Hidetaka, WATAI Masatoshi, ARAKAWA Fumihiro, OGASAWARA Takeshi, NISHIHARA Rikuka, KATO Hisashi, YAMAUCHI Atsushi, TAKAHATA Yoshihisa, MORIMATSU Fumiki, MAMEGOSHI Shinichi, MURAOKA Shiroo, HONJOH Tsutomu, WATANABE Takahiro, WAKUI Chiseko, IMAMURA Tomoaki, TOYODA Masatake, MAITANI Tamio, Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 44, 5, 213, 219,   2003 10 25 , 10.3358/shokueishi.44.213, http://ci.nii.ac.jp/naid/10031169287
    Summary:Inter-laboratory evaluation studies were conducted for the notified ELISA methods for allergic substances (Egg). Standard extracts of egg spiked in extracts of sausage, sauce, cookie, bread and cereal at a level of 5-20 ng/mL as the sample solution were analyzed in replicate in 10 laboratories. Coefficients of variation (CVs) of all three ELISA methods using an Egg Protein ovalbumin ELISA Kit (ovalbumin kit), an Egg Protein ovomucoid ELISA Kit (ovomucoid kit) and a FASTKITTM Egg ELISA kit (Egg ELISA kit) were mostly less than 10%. Mean recoveries of the standard extract of egg were over 40% in the three ELISA methods. Repeatability relative standard deviations of egg standard solution in five food extracts were in the ranges of 18.7-25.5%, 18.6-41.8%, 21.3-43.3% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. Reproducibility relative standard deviations of egg standard solution in five food extracts were 16.8-35.1%, 19.6-35.8%, 24.7-51.1% for the ovalbumin kit, the ovomucoid kit and the Egg ELISA kit, respectively. The detection limits of all the ELISA methods were 4-5 ng/mL in sample solutions. These results suggested that the notified ELISA methods are reliable and reproducible for the inspection of egg protein levels in extracts of sausage, sauce, cookie, bread and cereal.
  • Signal Transduction by Phosphoinositide-Specific Phospholipase C, MORIYAMA Tatsuya, KITO Makoto, KAGAKU TO SEIBUTSU, 30, 11, 714, 722,   1992 , 10.1271/kagakutoseibutsu1962.30.714, http://ci.nii.ac.jp/naid/130003633507
  • Purification and Characterization of Diaylglycerol Lipase from Human Platelets., Journal of Biochemistry, 125, 6, 1077,   1999
  • The mechanism of Arachidonic Acid Release in Collagen-activated Human Platelets., Bioscience, Biotechnology and Biochemistry, 58, 1, 93, 98,   1994 , 10.1271/bbb.58.93
  • Elevation of Cytosolic Free Ca2+ is Directly Evoked by Thromboxane A2 in Human Platelets during Activation with Collagen, Journal of Biochemistry, 103, 6,   1988

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Analysis of changes of pollenosis-related class-2 allergen levels in major agricultural products., Tatsuya MORIYAMA, The major pan-allergens, Betv1 homolog, profilin,and thaumatin-like protein, were cloned and expressed in bacteria. Obtained recombinant allergen proteins were used for antibody production. Produced antibodies were used for the analysis of changes of these pollenosis-related class 2 allergen levels in major agricultural products. These allergen levels were drastically changed for the variation of species, methods of culture, processing methods, cooking methods. In addition, causative allergens were determined in the various agricultual products.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Studies on the physiological role of resistin secreted from adipocytes and the screening of food factors affecting the resistin secretion, Tatsuya MORIYAMA, The lifestyle disease increases, and it has become the serious social problem recently. Improving “Insulin resistance" has a significant roles for prevention and the improvement of the lifestyle disease. Some hormone or mediator molecules have been discovered as a molecule related to this insulin resistance.The molecule called “resistin" secreted from adipocyte was reported as a responsibility molecule that caused the insulin resistance several years ago. Then, we tried to find the food ingredient that influences the physiological effect of this resistin and the expression and the amount of the circulation of resistin in this research. Moreover, adiponectin was also examined. We succeeded to produce the specific antibodies for resistin. Then, the sandwich ELISA system was constructed by using the specific antibody of resistinNext, it was clarified that the medium to differentiate 3T3-L1 cell that was the cultured adipocyte enough was examined, and we found that the resistin protein was secreted from this cell to the medium actually by using the sandwich ELISA system. The secretion of adiponectin was similarly confirmed. Then, it was found that the secretion of resistin was inhibited by the teaflavones etc. as a result of examining whether the food ingredient influenced the secretion of these important adipocytokines and became clear. In addition, revitalization that adjusted the secretion of these adiponectin was seen in some food ingredients. It can be called the clarification of the food ingredient that can adjust the secretion of resistin an important finding.
  • Study on the Food Factors regulating lipid metabolism.
  • Physiological regulation by food factors
  • Study on the food allergy and allergen
  • Study on the lipid metabolism in cells