KINDAI UNIVERSITY


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ANO Takashi

Profile

FacultyDepartment of Biotechnological Science / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/882-ano-takashi.html
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Last Updated :2020/09/30

Education and Career

Education

  •  - 1986 , Osaka University, Graduate School, Division of Engineering
  •  - 1981 , Osaka University, School of Engineering

Academic & Professional Experience

  •   2014 , Faculty of Biology-Oriented Science and Technology, Kindai University
  • Associate Professor, Tokyo Institute of Technology Chemical Resources Laboratory, Resources Recycling Process Laboratory, Resources Recycling Process Laboratoty Resources Recycling Process Laboratory

Research Activities

Research Areas

  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering
  • Life sciences, Applied biochemistry
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering
  • Environmental science/Agricultural science, Environmental policy and society
  • Environmental science/Agricultural science, Environmental impact assessment

Research Interests

  • microbial fuel cell, Environmental Microbiology, Recycling of Organic Materials, Biofertilizer, Biocontrol, Microbiology

Published Papers

  • Components of rice husk biochar in promoting the growth, sporulation and iturin A production of Bacillus sp. strain IA., Ebe S, Ohike T, Okanami M, Ano T, Zeitschrift fur Naturforschung. C, Journal of biosciences, Zeitschrift fur Naturforschung. C, Journal of biosciences, 74(7-8), 211 - 217, Jul. 2019 , Refereed
  • Promotion of lipopeptide antibiotic production by Bacillus sp. IA in the presence of rice husk biochar., Ebe S, Ohike T, Matsukawa T, Okanami M, Kajiyama S, Ano T, Journal of pesticide science, Journal of pesticide science, 44(1), 33 - 40, Feb. 2019 , Refereed
  • Biological Control Potential of Streptomyces sp. AR10 Producing Albocycline Isolated from Soil around Ant Nest, ANO TAKASHI, Journal of Agricultural Science, Journal of Agricultural Science, 10(3), 54 - 61, Feb. 2018 , Refereed
  • In vitro and in vivo assay for assessment of the biological control potential of Streptomyces sp. KT.,, ANO TAKASHI, Journal of Plant Studies, Journal of Plant Studies, 7(1), 10 - 18, Jan. 2018 , Refereed
  • Improved Performance of Soil Microbial Fuel Cell by Adding Earthworms, ANO TAKASHI, Journal of Renewable Energy and Environment, Journal of Renewable Energy and Environment, 4(2&3), 33 - 38., Dec. 2017 , Refereed
  • Biocontrol of Rhizoctonia solani K1 by Iturin A Producer Bacillus subtilis RB14 Seed Treatment in Tomato Plants, Zohora Umme Salma, Ano Takashi, Rahman Mohammad Shahedur, ADVANCES IN MICROBIOLOGY, ADVANCES IN MICROBIOLOGY, 6(6), 424 - 431, May 2016 , Refereed
  • Screening of biocontrol bacterial strains against fungal plant pathogens from endophytes., 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 84, Aug. 2012
  • Isolation of suppressive bacteria from fermented soybean, natto, as candidates for microbial pesticide., 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 46, Aug. 2012
  • Improvement of lipopeptide antibiotic iturin A production using novel peptone of fish protein medium., 岡南 政宏, 阿野 貴司, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 34, Aug. 2012
  • Screening of biocontrol bacterial strains against fungal plant pathogens from endophytes., 大池 達矢, 真国 紘平, 岡南 政宏, 阿野 貴司, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 84, Aug. 2012
  • Isolation of suppressive bacteria from fermented soybean, natto, as candidates for microbial pesticide., 村田大地, 澤野沙耶佳, 大池達矢, 岡南 政宏, 阿野 貴司, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 5th International Symposium on Environmental Economy and Technology, Extended Abstracts, 46, Aug. 2012
  • Production of iturin A through glass column reactor (GCR) from soybean curd residue (okara) by Bacillus subtilis RB14-CS under solid state fermentation (SSF)., 岡南 政宏, 阿野 貴司, Advances in Bioscience and Biotechnology, Advances in Bioscience and Biotechnology, 3, 143 - 148, Apr. 2012 , Refereed
  • A core sequence within a transcriptional activation domain of the aryl hydrocarbon receptor AhR, (28), 1 - 11, Sep. 2011
  • A core sequence within a transcriptional activation domain of the aryl hydrocarbon receptor AhR, 岡南 政宏, 藤谷 純也, 阿野 貴司, 近畿大学生物理工学部紀要, 近畿大学生物理工学部紀要, (28), 1 - 11, Sep. 2011
    Summary:マウスのダイオキシン受容体型転写因子AhRを酵母内で発現させて機能解析した結果、転写活性化を担う61アミノ酸残基からなる領域を見出した。さらに、この領域を介した転写活性化のコアクティベーターとしてPCAF複合体が関与していることを示唆する結果を示した。
  • Utilization of fish protein in submerged and biofilm fermentation of Bacillus subtilis for production of lipopeptide antibiotic iturin A, Proceedings of International Conference on Antimicrobial Research (ICAR2010), Proceedings of International Conference on Antimicrobial Research (ICAR2010), 295, Nov. 2010
  • Utilization of fish protein in submerged and biofilm fermentation of Bacillus subtilis for production of lipopeptide antibiotic iturin A, Umme Salma Zohora, Abdul Wahab Khan, Masahiro Okanami, Mohammad, Shahedur Rahman, 阿野 貴司, 岡南 政宏, Proceedings of International Conference on Antimicrobial Research (ICAR2010), Proceedings of International Conference on Antimicrobial Research (ICAR2010), 295, Nov. 2010
    Summary:微生物の培養に用いられるペプトンとして、動物タンパク質であるミルクに由来するものと植物タンパク質であるダイズに由来するものが知られている。第三のタンパク源として海洋性資源に着目し、魚に由来するタンパク質を用い微生物の培養を試みた。その結果、良好な生育が認められたばかりでなく、生産指標として用いた抗生物質生産能としても高いものが観察され有望な資源であることが示された。(英文)
  • Biosurfactant production by Bacillus subtilis using pentose carbohydrate., 4th International Symposium on Environmental Economy and Technology, Extended Abstracts, 4th International Symposium on Environmental Economy and Technology, Extended Abstracts, Jun. 2010
  • Utilization of pentose carbohydrate and production of iturin A by Bacillus subtilis, ISEET-2010 The 4th International Symposium on Environmental Economy and Technology Extended Abstract, ISEET-2010 The 4th International Symposium on Environmental Economy and Technology Extended Abstract, 58 - 60, Jun. 2010
  • Biosurfactant production by Bacillus subtilis using pentose carbohydrate., Abdul Wahab Kahn, 岡南 政宏, 阿野 貴司, Mohammad Shahedur Rahman Umme Salma Zohora, 4th International Symposium on Environmental Economy and Technology, Extended Abstracts, 4th International Symposium on Environmental Economy and Technology, Extended Abstracts, Jun. 2010
  • Utilization of pentose carbohydrate and production of iturin A by Bacillus subtilis, Abdul Wahab Khan, Mohammad, Shahedur Rahman, Umme Salma Zohora, 阿野 貴司, 岡南 政宏, ISEET-2010 The 4th International Symposium on Environmental Economy and Technology Extended Abstract, ISEET-2010 The 4th International Symposium on Environmental Economy and Technology Extended Abstract, 58 - 60, Jun. 2010
    Summary:微生物の発酵生産において六炭糖が一般的に用いられている。食糧と競合せず、大量に入手可能な未利用資源としてヘミセルロースを構成するキシロースのような五炭糖が考えられる。しかし、五炭糖を資可できる微生物は限られているため、枯草菌を用いた五炭糖の利用と物質生産を試みた。その結果、良好な生育と有用物質であるサーファクチンの生産が認められた。
  • A general consideration toward the sustainable society, (7), 33 - 35, Mar. 2009
  • A general consideration toward the sustainable society, 阿野 貴司, 埼玉工業大学先端科学研究所アニュアルレポート, 埼玉工業大学先端科学研究所アニュアルレポート, (7), 33 - 35, Mar. 2009
    Summary:多くの環境問題を抱える現代社会が、持続可能な社会に変換するために必要な条件について、まとめと考察を行った。先ず、資源の有限性を理解し深く認識することが、省資源のライフスタイルにつながることを指摘した。また、生態系やその一部としての人間社会の永続性は、森林の健全性によりもたらせること、特に豊かな土壌形成が食糧生産と文明の持続性の観点からも重要であることをまとめた。(英文)
  • Potential application of oligotrophic bacteria as biocontrol agents, Tatsuya Ohike, Tetsuya Matsukawa, Masahiro Okanami, Shin'ichiro Kajiyama, Takashi Ano, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 248, Aug. 2014 , Refereed
  • Isolation of antifungal bacteria from Japanese fermented soybeans, natto, Daichi Murata, Sayaka Sawano, Tatsuya Ohike, Masahiro Okanami, Takashi Ano, Journal of Environmental Sciences (China), Journal of Environmental Sciences (China), 25(1), S127 - S131, 2013 , Refereed
    Summary:An inhibitory effect of a traditional Japanese fermented food, natto, was found against plant pathogens such as Rhizoctonia solani and Fusarium oxysporum, and the bacteria which showed inhibition were isolated from the natto. Among isolated bacteria, BC-1 and GAc exhibited a strong antagonistic effect in vitro against plant pathogens on an agar medium. The supernatant of bacterial culture also showed strong activity against R. solani, which meant the antimicrobial substances were produced and secreted into the medium. Both of the bacteria were estimated as Bacillus amyloliquefaciens from a partial sequence of the 16s rRNA gene. High performance liquid chromatography analysis clearly showed the production of the lipopeptide antibiotic iturin A by BC-1 and GAc. © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Improvement of production of lipopeptide antibiotic iturin A using fish protein, Umme Salma Zohora, Mohammad Shahedur Rahman, Abdul Wahab Khan, Masahiro Okanami, Takashi Ano, Journal of Environmental Sciences (China), Journal of Environmental Sciences (China), 25(1), S2 - S7, 2013 , Refereed
    Summary:To enhance the production of lipopeptide antibiotic iturin A, nutrient contents of the culture mediums were investigated in both submerged and biofilm fermentations. As a carbon source maltose and as nitrogen source, fish protein was used. In submerged fermentation maltose uptake was found lower (12%) compared to biofilm fermentation (15%) that was associated with higher cellular growth in biofilm. However, requirement of nitrogen (fish protein) concentration was found similar in both submerged and biofilm fermentations. Production of iturin A in submerged fermentation with 12% maltose and 5% fish protein was 4450 mg/L, and in biofilm fermentation it was 5050 mg/L when 15% maltose and 5% fish protein was used. © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Screening of endophytic bacteria against fungal plant pathogens, Tatsuya Ohike, Kohei Makuni, Masahiro Okanami, Takashi Ano, Journal of Environmental Sciences (China), Journal of Environmental Sciences (China), 25(1), S122 - S126, 2013 , Refereed
    Summary:Bacterial endophytes were found from 6 plant leaves among 35 plant leaves screened. Two of the isolated bacteria showed antagonistic activity against fungal plant pathogens. An isolate named KL1 showed the clear inihibition against plant pathogens, Fusarium oxysporum and Rhizoctonia solani, on PDA as well as TSA plate. Supernatant of the bacterial culture also showed the clear inhibition against the fungal growth on the plate and the antibiotic substance was identified as iturin A by HPLC analysis. KL1 was identified as Bacillus sp. from the 16S rRNA gene analysis. Very thin hyphae of R. solani was miccroscopically observed when the fungus was co-cultivated with KL1. © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Production of surfactin using pentose carbohydrate by Bacillus subtilis, Abdul Wahab Khan, Mohammad Shahedur Rahman, Umme Salma Zohora, Masahiro Okanami, Takashi Ano, Journal of Environmental Sciences, Journal of Environmental Sciences, 23(SUPPL.), S63 - S65, Jun. 2011 , Refereed
    Summary:Interest in microbial surfactants has been steadily increasing in recent years due to their diversity, mass production possibility, selectivity, performance under extreme conditions and potential applications in environmental protection. In this study two pentose sugars (xylose and arabinose) were investigated for the submerged fermentation (SmF) of Bacillus subtilis in surfactant production medium for bio-surfactant surfactin production. An excellent vegetative growth of B. subtilis (× 10 10 CFU/mL) was observed for xylose and arabinose containing medium which were comparable to glucose supplemented medium. Low growth (× 10 8 CFU/mL) was found when medium was not supplemented with any of the sugars. Surfactin production in xylose, arabinose and glucose containing medium was 2700, 2600 and 2000 mg/L, respectively, whereas, medium without any sugar showed low surfactin (700 mg/L) production. These results clearly indicate the effect of pentose sugars on production of surfactin. Gradual depletion of the xylose and arabinose were confirmed by HPLC analysis during the growth phase of the strain that ultimately produced the surfactin. © 2011 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Study of Submerged and Biofilm Fermentation of Bacillus subtilis using Fish Protein for Production of Lipopeptide Antibiotic Iturin A, Umme Salma Zohora, Abdul Wahab Khan, Masahiro Okanami, Takashi Ano, Mohammad Shahedur Rahman, SCIENCE AND TECHNOLOGY AGAINST MICROBIAL PATHOGENS: RESEARCH, DEVELOPMENT AND EVALUATION, SCIENCE AND TECHNOLOGY AGAINST MICROBIAL PATHOGENS: RESEARCH, DEVELOPMENT AND EVALUATION, 190 - 195, 2011 , Refereed
    Summary:Iturin A is an environmentally safe biocontrol agent produced by Bacillus subtilis as a secondary metabolite. Generally iturin A is produced in conventional submerged fermentation. Recently, B. subtilis has received a huge interest for its nature to develop into biofilm as it shows significantly independent genetic and morphological development in biofilm compared to its planktonic culture. In this study it was attempted to compare the production of iturin A in submerged with that in biofilm fermentation using novel marine fish protein as a medium component. When fish protein was compared with commercially available peptones, it was observed that the microbial growth and iturin A productions were similar to those in the medium containing Polypepton S (originated from soybean) and higher than those in the medium containing Polypepton (originated from casein). Quicker cellular growth and secondary metabolite production was observed in submerged fermentation whereas slower but higher cellular growth and iturin A production was found in biofilm fermentation.
  • In Vitro Communities Derived from Oral and Gut Microbial Floras Inhibit the Growth of Bacteria of Foreign Origins, Xuesong He, Yan Tian, Lihong Guo, Takashi Ano, Renate Lux, David R. Zusman, Wenyuan Shi, MICROBIAL ECOLOGY, MICROBIAL ECOLOGY, 60(3), 665 - 676, Oct. 2010 , Refereed
    Summary:The gastrointestinal (GI) tract is home to trillions of microbes. Within the same GI tract, substantial differences in the bacterial species that inhabit the oral cavity and intestinal tract have been noted. While the influence of host environments and nutritional availability in shaping different microbial communities is widely accepted, we hypothesize that the existing microbial flora also plays a role in selecting the bacterial species that are being integrated into the community. In this study, we used cultivable microbial communities isolated from different parts of the GI tract of mice (oral cavity and intestines) as a model system to examine this hypothesis. Microbes from these two areas were harvested and cultured using the same nutritional conditions, which led to two distinct microbial communities, each with about 20 different species as revealed by PCR-based denaturing gradient gel electrophoresis analysis. In vitro community competition assays showed that the two microbial floras exhibited antagonistic interactions toward each other. More interestingly, all the original isolates tested and their closely related species displayed striking community preferences: They persisted when introduced into the bacterial community of the same origin, while their viable count declined more than three orders of magnitude after 4 days of coincubation with the microbial flora of foreign origin. These results suggest that an existing microbial community might impose a selective pressure on incoming foreign bacterial species independent of host selection. The observed inter-flora interactions could contribute to the protective effect of established microbial communities against the integration of foreign bacteria to maintain the stability of the existing communities.
  • Production characteristics of lipopeptide antibiotics in biofilm fermentation of Bacillus subtilis, Mohammad Shahedur RAHMAN, Takashi ANO, Journal of Environmental Sciences, Journal of Environmental Sciences, 21(1), S36 - S39, 2009 , Refereed
    Summary:In biofilm fermentation, from the very early moments, surfactin was produced along with the biofilm development in the lipopeptide antibiotic production medium by using Bacillus subtilis. However, almost no iturin A was produced in its first 24 hours of cultivation and the production of iturin A began much later. Volumes of the nutrient medium and available surface area of the biofilm reactors were found to be important with the relative production of these two antibiotics. Production of iturin A was increased from 12 mg to about 50 mg per reactor when the culture size was increased from 5 mL to 20 mL, as the depth of the medium was increased. The production level was saturated thereafter with larger volumes. On the other hand, surfactin production was remained similar, which was about 10 mg per reactor, from all the 5 mL to 80 mL of biofilm culture. Optimized temperature for iturin A and surfactin production was observed at 25 and 37°C, respectively. In the biofilm fermentation, production of surfactin was increased when the incubation temperature was increased within the temperature range of 25 to 37°C, on the other hand, iturin A production was gradually decreased with the increase of the incubation temperature. © 2009 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Application of malt residue in submerged fermentation of Bacillus subtilis, Abdul Wahab KHAN, Mohammad Shahedur RAHMAN, Takashi ANO, Journal of Environmental Sciences, Journal of Environmental Sciences, 21(1), S33 - S35, 2009 , Refereed
    Summary:Malt residue is a common waste or byproduct from beer industries after brewing and milling of malted barley. In this work, Bacillus subtilis RB14 was used to study the microbial growth and production of secondary metabolites like lipopeptide antibiotic iturin A in the malt residue for its effective recycling. B. subtilis RB14 could grow in submerged fermentation of malt residue and significant growth (109 CFU/mL) was observed without any supplementation. In submerged fermentation iturin A production using malt residue was about 170 mg/L, which was found to be higher than its production in No.3 (Polypepton, glucose, KH2PO4, MgSO4·7H2O) medium where production was about 120 mg/L. More than 600 mg/L of iturin A production was observed when malt residue was combinedly used with No.3 medium. This production was significantly higher than their summation of their individual production. However, the growth of B. subtilis in combined medium was found to be similar to that of the submerged fermentation in simple malt residue. Therefore, the remarkable enhancement in production of iturin A in supplemented malt residue was attributed to the nutrients supplied from No.3 medium. © 2009 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Biofilm formation and lipopeptide antibiotic iturin A production in different peptone media, Umme Salma ZOHORA, Mohammad Shahedur RAHMAN, Takashi ANO, Journal of Environmental Sciences, Journal of Environmental Sciences, 21(1), S24 - S27, 2009 , Refereed
    Summary:Biofilm fermentation is a newly developed promising technique in fermentation technology. In this study no.3 and no.3S media have been used for the lipopeptide antibiotic iturin A production by Bacillus subtilis RB14. The main component of no.3 and no.3S media is Polypepton and Polypepton S, respectively. B. subtilis RB14 produces thick stable biofilm and high amount of iturin A in no.3S medium. Whereas, impaired biofilm formation and lower iturin A production was observed in no.3 medium. From the analytical information it was observed that the amounts of metal ions, such as K+, Ca2+ and Mn2+, cysteine and cellulose are lower in Polypepton compared to the Polypepton S. To investigate their effect on biofilm formation and iturin A production cysteine, cellulose, K+, Ca2+ and Mn2+ were added respectively into the no.3 medium at similar amount that Polypepton S contains. It was observed that individual addition of K+, Ca2+, cysteine and cellulose had no effect on biofilm formation, cellular growth induction or iturin A production. However, when Mn2+ was supplemented in no.3 medium, biofilm development was restored with an improved production of iturin A. Finally, combined addition of investigated substances into the no.3 medium resulted with highly folded, thick biofilm with high cellular growth and iturin A production compared to the original no.3 medium. © 2009 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Solid state fermentation of lipopeptide antibiotic iturin A by using a novel solid state fermentation reactor system, Takashi ANO, Guang Yuan JIN, Shinji MIZUMOTO, RAHMAN Mohammad Shahedur, Kasumasa OKUNO, Makoto SHODA, Journal of Environmental Sciences, Journal of Environmental Sciences, 21(1), S162 - S165, 2009 , Refereed
    Summary:A new solid state fermentation reactor (SSFR) for solid substrate was used for the production of lipopeptide antibiotic iturin A using Bacillus subtilis RB14-CS. Solid state fermentation (SSF) is the technique of cultivation of microorganisms on solid and moist substrates in the absence of free water. SSF has shown much promise in the development of several bioprocesses and products because of their several advantages like absence of free water that allows simplified downstream processing and low cost. SSFR allows agitation of the SSF culture with improved temperature control and air supply. Interestingly, when okara, the widely available waste product from the tofu industries, was used as the solid substrate for the SSFR, no iturin A production was observed. However, without agitation, production of iturin A was observed in the SSFR but the production level remained low. The low production of iturin A was found to be due to the heat generation and excess temperature rise inside the reactor system during the fermentation process. Maintaining the temperature within a range of 25-30°C, production of iturin A was significantly improved in the SSFR. This was comparable to the laboratory scale production, and signifies the potential application of the SSFR for SSF. © 2009 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
  • Production of iturin A homologues under different culture conditions, Noriyasu IWASE, Mohammad Shahedur RAHMAN, Takashi ANO, Journal of Environmental Sciences, Journal of Environmental Sciences, 21(1), S28 - S32, 2009 , Refereed
    Summary:Iturin A is a cyclic lipopeptide antibiotic and eight different kinds of iturin A have been reported based on its alkyl side chains. As iturin A is a promising biocontrol agent, total production of iturin A was tried to enhance and comparative production of its homologues was investigated by using different nitrogen and carbon sources. When Polypepton S and defatted soybean meal were used, total production as well as the ratio of the iturin A homologues were similar. However, production of iturin A was relatively lower and also the ratio of the iturin A homologues was different when Polypepton was used, where A2 was decreased and A4 was increased. Production ratio of the iturin A homologues was similar for the carbon sources like maltose, mannitol, sucrose and starch but relative production of iturin A2 was much enhanced compared to A3 when lactose or galactose was used. Interestingly production ratio of A4 was increased and A2 and A3 were decreased when no additional carbon source was used, and similar tendency was observed in the homologue ratio with glucose and fructose. Production of iturin A homologue A6 was significantly increased whereas A2 and A3 were decreased when defatted rapeseed cake was used. Utilization of different amino acids did not show significant differences in their production of the iturin A homologues. Oxygen supply found to be the factor affecting the production of iturin A homologues when it was investigated in a varied culture volume size and shaking speed. A2 found to be increased with increased oxygen supply where the production of A3 was affected inversely. © 2009 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.

Books etc

  • Science and Technology Against Microbial Pathogens, Joint author, World Scientific,   2011 09
  • Bacterial growth under strong magnetic field electricity and magnetism in biology and medicine, Kuwar Academic/Plenum Publishers,   1999
  • Recombinant Microbes for Industrial and Agricultural Applications (分担), Marcel Dekker, Inc,   1993

Conference Activities & Talks

  • The mechanisms for plant growth promotion of Pseudomonas fluorescens A-2 on Arabidopsis thaliana,   2013 03
  • Microbial fuel cell mediated by wine yeast., International Union of Microbial Societies 2011,   2011 09 , International Union of Microbial Societies 2011
  • Isolation of phorbol ester degrading microbes for Jatropha bio-diesel production., International Union of Microbial Societies 2011,   2011 09 , International Union of Microbial Societies 2011
  • Isolation and characterization of bacteria as a candidate for biological control., International Union of Microbial Societies 2011,   2011 09 , International Union of Microbial Societies 2011
  • Biosurfactant production by Bacillus subtilis using pentose carbohydrate, ISEET-2010 The 4th International Symposium on Environmental Economy and Technology,   2006 06 , ISEET-2010 The 4th International Symposium on Environmental Economy and Technology
  • Microbes and environment, The 2nd China-Japan-Korea joint symposium on environmental economy and technology,   2007
  • Application of Secondary Emission Electron Gun on Bacterial Inactivation, 3rd Asia-Pacific International Symposium on the Basic and Application of Plasma Technology,   2003
  • Application of secondary emission electron gun on bacterial inactivation, 3rd Asia-Pacific International Symposium on the Basic and Application of Plasma Technology (APSBAPT),   2003
  • Effect of high magnetic fields on GASP(Growth Advantage in Stationary Phase),   2000
  • High magnetic field enhances rpoS transcription of Escherichia coli in stationary phase.,   1999
  • Effect of storng magnetic fields on the bacterial growth in SBS.,   1997
  • Characteristics of the supernatant of E. coli cultivated under high magnetic field.,   1997
  • Effect of homogeneous and inhomogeneous high magnetic fields on bacterial growth measured under a newly developed superconducting magnet biosystem, Annual International Conference of the IEEE Engineering in Medicine and Biology,   1996

Misc

  • 減菌可能な一槽式微生物燃料電池の作製, 福本悠地, 雪本寛, 江邉正平, 大池達矢, 岡南政宏, 岡南政宏, 阿野貴司, 阿野貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University, 42, 45‐51,   2018 10 31 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802256011346474
  • PGPBがもつ植物生長促進作用と植物病原菌感染防除効果の評価, 澤田祥子, 丸山大地, 吉田悠生, 吉水芳織, 西山紋加, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 植物の生長調節, 53, Supplement, 107,   2018 10 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802214407828758
  • 土壌微生物燃料電池における発電と植物生長, 雪本寛, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 植物の生長調節, 53, Supplement, 106,   2018 10 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802243690532411
  • 籾殻燻炭における植物生長メカニズムの解明, 久野龍之介, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 植物の生長調節, 53, Supplement, 105,   2018 10 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802275242047474
  • 微生物発電に伴う有用物質生産の試み, 福本悠地, 雪本寛, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 70th, 153,   2018 08 07 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802223628641450
  • 放線菌由来揮発性有機化合物が植物病原菌に与える影響, 前田みのり, 大池達矢, 岡南政宏, 松川哲也, 梶山慎一郎, 阿野貴司, 日本生物工学会大会講演要旨集, 70th, 165,   2018 08 07 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802274262418205
  • 燻炭がBacillus属細菌IA株の生育を促進するメカニズムの解明 3, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 70th, 152,   2018 08 07 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802276573669603
  • ミミズが土壌微生物燃料電池に及ぼす効果, 雪本寛, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 70th, 153,   2018 08 07 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802286909071477
  • 塩素系薬剤によるリステリア損傷菌のThin agar layer法とFlow cytometry法による検出比較, 井上あやの, 阿野貴司, 山脇伸行, 白木琢磨, 泉秀実, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University, 41, 13‐23,   2018 02 28 , http://jglobal.jst.go.jp/public/201802221764808079
  • Stenotrophomonas maltophiliaエステラーゼの大量発現と精製および機能向上の試み, 鳥居誉, 田中彩, 諸見直樹, 田端菜央, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本分子生物学会年会プログラム・要旨集(Web), 41st, ROMBUNNO.3P‐0042 (WEB ONLY),   2018 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802274925257357
  • Pseudomonas fluorescens A‐2株によるシロイヌナズナの生長促進作用メカニズムの解析, 奥野圭祐, 中岡知規, 澤田祥子, 立川雄樹, 小森大輔, 吉水芳織, 大浦麻里, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 植物の生長調節, 52, Supplement, 80,   2017 10 06 , http://jglobal.jst.go.jp/public/201702236020762333
  • 植物生長促進細菌が植物の生長に与える影響, 中岡知規, 澤田祥子, 西山紋加, 小林誠治, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 植物の生長調節, 52, Supplement, 79,   2017 10 06 , http://jglobal.jst.go.jp/public/201702237338692656
  • 植物生長促進細菌の機能とその利用, 中岡知規, 澤田祥子, 西山紋加, 小林誠治, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本農芸化学会中四国支部講演会講演要旨集(Web), 49th, 82 (WEB ONLY),   2017 09 21 , http://jglobal.jst.go.jp/public/201702255099275086
  • Stenotrophomonas maltophiliaエステラーゼの大量発現と精製の試み, 鳥居誉, 田端菜央, 奥野圭祐, 阿野貴司, 岡南政宏, 阿野貴司, 岡南政宏, 日本生物工学会大会講演要旨集, 69th, 120,   2017 08 08 , http://jglobal.jst.go.jp/public/201702226699639785
  • 高塩濃度条件下で生育可能な微生物による電力生産, 雪本寛, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 69th, 302,   2017 08 08 , http://jglobal.jst.go.jp/public/201702227274129469
  • 新規の植物生長促進細菌の単離と評価, 澤田祥子, 中岡知規, 西山紋加, 吉水芳織, 黒田雄亮, 阿野貴司, 岡南政宏, 阿野貴司, 岡南政宏, 日本生物工学会大会講演要旨集, 69th, 306,   2017 08 08 , http://jglobal.jst.go.jp/public/201702230386902773
  • 植物生長促進細菌の単離と微生物農薬としての利用, 中岡知規, 西山紋加, 小林誠治, 阿野貴司, 岡南政宏, 阿野貴司, 岡南政宏, 日本生物工学会大会講演要旨集, 69th, 306,   2017 08 08 , http://jglobal.jst.go.jp/public/201702257925389986
  • 放線菌由来生理活性物質が植物および植物病原菌に与える影響, 前田みのり, 大池達矢, 岡南政宏, 松川哲也, 梶山慎一郎, 阿野貴司, 日本生物工学会大会講演要旨集, 69th, 321,   2017 08 08 , http://jglobal.jst.go.jp/public/201702278589648865
  • 燻炭抽出液によるBacillus amyloliquefaciens IA株のiturinA生産促進, 久野龍之介, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 69th, 310,   2017 08 08 , http://jglobal.jst.go.jp/public/201702279677358386
  • 植物生長促進細菌の単離と養液栽培における利用, 小林誠治, 中岡知規, 宮澤知愛, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.3C25a05 (WEB ONLY),   2017 03 05 , http://jglobal.jst.go.jp/public/201702222802885324
  • 燻炭により生育が促進する微生物の探索と抗真菌活性の比較, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.2J28a06 (WEB ONLY),   2017 03 05 , http://jglobal.jst.go.jp/public/201702255580591751
  • 植物生長促進作用に関与するPseudomonas fluorescens遺伝子の単離, 中岡知規, 澤田祥子, 大浦麻里, 田中雄矢, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.4C18a05 (WEB ONLY),   2017 03 05 , http://jglobal.jst.go.jp/public/201702282981544880
  • ミミズが土壌成分と植物生長に与える効果の研究, SU Tingxuan, 江邉正平, 大池達矢, 岡南政宏, 岡南政宏, 阿野貴司, 阿野貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 39, 27‐38,   2017 02 28 , http://jglobal.jst.go.jp/public/201702245158142368
  • 〈Original Papers〉Studies on the effect of earthwoms on soil characteristics and plant growth, 蘇 霆軒, 江邉 正平, 大池 達矢, 岡南 政宏, 阿野 貴司, 近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University, 39, 27, 38,   2017 02 28 , http://ci.nii.ac.jp/naid/120006028233
    Summary:[要旨] ミミズは土壌を摂食行動により団粒化し、さらに落ち葉などの植物リターを腸内で分解し土壌ヘ肥料成分を供給することで植物生長を促進すると考えられている。また、ミミズは多くの微生物と共生していることが知られており、ミミズ共生微生物も植物の生長に重要な役割を担っていると考えられている。本研究では、ミミズおよびミミズ共生微生物による植物生長促進効果の解明を目的として実験を行った。まず、近畿大学生物理工学部構内からミミズを採取し、土壌でのミミズ飼育試験およびミミズ飼育土壌を用いた植物栽培試験を行い、ミミズの種類と存在量が植物に与える影響を調査した。その結果、近畿大学構内に生息する4種類のミミズを同定し、植物栽培試験により一部のミミズが植物の生長を促進することが認められた。採取されたミミズの中でもへンイセイミミズは、飼育数が多いほど、植物生長を促進した。またミミズを2週間飼育した土壌の成分を分析したところ、飼育土壊における硝酸態イオン濃度の増加が認められ、ミミズは硝化能力を持つ微生物と共生していることが示唆された。そこで、ミミズ腸内において硝化に関わる微生物の存在を明らかにするため、ヘンイセイミミズから糞を回収し硝化菌検出用培地で培養したところ、アンモニウムイオンからの亜硝酸イオンと硝酸イオンの生成が認められた。[Abstract] Earthworms eat both plant litter and soil, and help plant growth by changing soil structure and increasing the nutrients of the soil. They also have a symbiotic relationship with various microorganisms, which led us to hypothesize if the microorganisms found in the earthworms play an important role in plant growth. In this study, we investigated the influence of earthworm type and quantity on plant growth using cultivated soils of the earthworms collected from the Kindai University campus (Faculty of Biology-Oriented Science and Technology in Wakayama). As a result, we identified 4 earthworm species, some of which showed growth promoting effect on plants. In additon, as the amount of earthworms Pheretima heteropoda increased, the growth promotion effect of earthworm-cultivating soil increased. Nitrate was increased in soils that cultivated earthworms for 2 weeks, suggesting that some of the microorganisms symbiotic in the earthworm may potentially have the ability of nitrification. To elucidate if there is nitrifying bacteria within the earthworms, we incubated the cast of earthworms. P.heteropoda in the medium for detecting nitrifying bacteria. As a result, we confirmed nitrite and nitrate production activity from ammonia in the earthworm cast.
  • 植物生長促進細菌によるシロイヌナズナの生長促進作用メカニズムの解析, 中岡知規, 澤田祥子, 大浦麻里, 田中雄也, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本生物工学会大会講演要旨集, 68th, 223,   2016 08 25 , http://jglobal.jst.go.jp/public/201602215931251373
  • アルボサイクリン生産放線菌を用いた微生物農薬の開発, 大池達矢, 前田みのり, 松川哲也, 松川哲也, 岡南政宏, 岡南政宏, 梶山慎一郎, 梶山慎一郎, 阿野貴司, 阿野貴司, 日本生物工学会大会講演要旨集, 68th, 243,   2016 08 25 , http://jglobal.jst.go.jp/public/201602229232187172
  • エステラーゼを生産する環境細菌の単離, 奥野圭祐, 橋本明里, 鳥居誉, 前田真美, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本生物工学会大会講演要旨集, 68th, 227,   2016 08 25 , http://jglobal.jst.go.jp/public/201602233139199223
  • Stenotrophomonas maltophiliaエステラーゼの遺伝子クローニングと大量発現, 鳥居誉, 奥野圭祐, 朝倉紗也栞, 大浦麻里, 阿野貴司, 阿野貴司, 岡南政宏, 岡南政宏, 日本生物工学会大会講演要旨集, 68th, 285,   2016 08 25 , http://jglobal.jst.go.jp/public/201602236457136016
  • Streptomyces sp.KT株の微生物農薬としての可能性, 前田みのり, 大池達矢, 岡南政宏, 岡南政宏, 松川哲也, 松川哲也, 梶山慎一郎, 梶山慎一郎, 阿野貴司, 阿野貴司, 日本生物工学会大会講演要旨集, 68th, 243,   2016 08 25 , http://jglobal.jst.go.jp/public/201602290677061238
  • 燻炭がBacillus属細菌IA株の生育を促進するメカニズムの解明, 江邉正平, 大池達矢, 岡南政宏, 阿野貴司, 日本生物工学会大会講演要旨集, 68th, 243,   2016 08 25 , http://jglobal.jst.go.jp/public/201602291460009338
  • Screening of beneficial microorganisms from feces of captive animals in Osaka Tennoji Zoo, 江邉 正平, 芳野 美咲, 米田 奈央, 蘇 霆軒, 鷲尾 尚輝, 大池 達矢, 岡南 政宏, 阿野 貴司, 高見 一利, 宮下 実, 近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University, 37, 31, 38,   2016 05 31 , http://ci.nii.ac.jp/naid/120005823364
    Summary:本研究では有用微生物の探索を目的とし、様々な動物を飼育している動物園に着目した。天王寺動物園において生態や食性が異なる4種の飼育動物、アジアゾウ、キタジマキーウィ、コアラ、コウベモグラの糞を採取、スクリーニングを試みたところ、それぞれの動物の糞サンプルからマンガンを還元する微生物、炭酸固定・窒素固定を行う微生物、植物生長促進を行う微生物、植物病原菌に対して抑制能を示す放線菌、難分解性の多糖を分解する微生物の存在が認められた。このことから飼育動物の糞は有用微生物の単離源として有効であることを示した。The aim of this study is the isolation of beneficial microorganisms from the animal feces. Since the zoo is keeping a variety of animal species, the fecal samples were collected at the zoo from the following four animals which have different ecological feateures: Asian elephant(Elephas maximus), North Island brown kiwi (Apteryx mantelli), koala(Phascolarctos cinereus) and Japanese western mole (Mogera wogura). Isolation of beneficial microorganisms from the animal feces and activity tests have been performed by using agar media. As a result, all animal feces have shown the presence of beneficial microorganisms such as manganese reducing microorganisms, carbon-fixing microorganisms, nitrogen-fixing microorganisms, plant growth promoting microorganisms, actinomycetes that have antifungal activity against plant pathogenic fungi, and persistent polysaccharide degrading microorganisms. This study revealed that feces of captive animals have usefulness for screening of beneficial microorganisms.
  • 環境細菌が産生するエステラーゼの特性と遺伝子の解析, 朝倉 紗也栞, 前田 真美, 柳井 祐佳里, 阿野 貴司, 岡南 政宏, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2P0527], [2P0527],   2015 12
  • 環境細菌が産生するエステラーゼの特性と遺伝子の解析, 朝倉 紗也栞, 前田 真美, 柳井 祐佳里, 阿野 貴司, 岡南 政宏, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2P0527], [2P0527],   2015 12
  • 3P-144 An attempt at generating electricity during pyrrolnitrin biosynthesis, Taniguchi Yuki, Ebe Shohei, Ohike Tatsuya, Okanami Masahiro, Ano Takashi, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010009559
  • 3P-126 Growth enhancement of bacteria in the presence of biochar and their antibiotic production, Ebe Shohei, Ohike Tatsuya, Okanami Masahiro, Ano Takashi, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010009541
  • 2P-155 Isolation of antifungal active substance from actinomycetes, Uetsuki Yoshihiro, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010009242
  • 1P-071 Properties of an esterase produced by Stenotrophomonas maltophilia 4A, Okanami Masahiro, Yanai Yukari, Ooura Mari, Asakura Sayaka, Maeda Mami, Ano Takashi, 日本生物工学会大会講演要旨集, 67,   2015 09 25 , http://ci.nii.ac.jp/naid/110010008851
  • 発電を伴う有用物質生産の試み, TANIGUCHI YUKI, EBE SHOHEI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 67th, 306, 306,   2015 09 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502203658326420
  • Stenotrophomonas maltophilia 4Aが産生するエステラーゼの特性, OKANAMI MASAHIRO, OKANAMI MASAHIRO, YANAI YUKARI, OURA MARI, ASAKURA SAYAKA, MAEDA MAMI, ANO TAKASHI, ANO TAKASHI, 日本生物工学会大会講演要旨集, 67th, 106,   2015 09 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502204132130250
  • 放線菌由来抗菌活性物質の探索, UETSUKI YOSHIHIRO, OIKE TATSUYA, MATSUKAWA TETSUYA, OKANAMI MASAHIRO, KAJIYAMA SHIN'ICHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 67th, 213, 213,   2015 09 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502209849337129
  • 燻炭添加により増殖促進を示す細菌の単離と抗生物質生産, EBE SHOHEI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 67th, 302, 302,   2015 09 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502211824895571
  • 数種果実の微生物汚染源としての農業用水と農薬溶液の影響, KITADA KOSUKE, KOMOTO KEIKO, ANO TAKASHI, IZUMI HIDEMI, Mem Fac Biol Oriented Sci Technol Kinki Univ, 34, 34, 27, 33,   2014 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402235710614406
    Summary:[要旨] 和歌山県産の果実(カキ、ウンシュウミカン、ウメ)の各圃場を対象に, 農業用水, 農薬溶液および収穫果実の微生物汚染度を測定し, これらの栽培環境接触物が微生物汚染源として青果物に及ぼす影響を調査した. 農業用水に使用した水源の微生物汚染度は, 河川水が谷の湧水より1log程度高かった. さらに, 農業用水に農薬を溶解することで, 微生物数が最大で2log程度増加し, 微生物汚染度の増大が確認された. 農薬散布から3日後に収穫されたカキ果実では, 農業用水および農薬溶液から土壌由来あるいは植物病原性の7菌種が移行したと推定され, 農薬散布から8日後のウンシュウミカン果実(2菌種)および31日後のウメ果実(1菌種)よりも多いことが確認された. いずれのサンプルからも食中毒原因菌(verotoxin-producing Escherichia coli, Salmonella, Listeria monocytogenes)は検出されず, 農業用水および農薬溶液を介した微生物的食品安全危害はないことが示された. [Abstract] Microflora of field environments (agricultural water and pesticide solutions diluted with agricultural water) and several fruits (persimmons, satsuma mandarin, and Japanese apricots) were investigated to determine the potential of the field environment as a preharvest source of microbial contamination for the fruits. With agricultural water, the microbial counts in river water were 1 log higher than those counts in mountain spring water. When pesticide products were dissolved in agricultural water, the microbial counts increased by 2 logs. Seven species of soilborne or phytopathogenic bacteria andmolds detected on persimmon fruit harvested 3 days after pesticide application were also found in the agricultural water and pesticide solution, suggesting that the field environment could be a source of preharvest contamination. In comparison there were fewer microbial species found on satsuma mandarin fruit (2 species) and Japanese apricot fruit (1 species) harvested 8days and 31 days after pesticide application, respectively, that appeared to be transferred from the agricultural water and pesticide solutions. Since foodborne pathogens such as Salmonella, verotoxin-producing Escherichia coli, and Listeria monocytogenes were not detected in any of the fruit and environmental samples, it is assumed that there are no microbialfood safety hazards resulting from cross-contamination with the environment.
  • 酵母を用いた微生物燃料電池における発電力向上の試み, EBE SHOHEI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, Mem Fac Biol Oriented Sci Technol Kinki Univ, 34, 34, 15, 26,   2014 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402239314889449
    Summary:[要旨] 微生物燃料電池とは, 微生物が有機物を分解する過程で生じる電子を電気エネルギーとして回収する装置である. 微生物燃料電池は, 有機物から直接電気エネルギーを取り出すことが出来ることから, 廃水処理と組み合わせた利用が考えられている. 本研究では, モデル微生物であり, 数多くの食品にも利用されている酵母を用いた微生物燃料電池の開発を行った. そして酵母を用いた微生物燃料電池の発電効率を上げるため2つのアプローチを試みた. それらは酵母と乳酸菌を共培養した際に形成される複合バイオフィルムと微生物燃料電池を組み合わせたものと、燻炭による代謝促進と微生物燃料電池を組み合わせたものである. 本研究により, 強い複合バイオフィルム形成を行う酵母と乳酸菌の組み合わせを特定できた. この組み合わせを微生物燃料電池に組み込むことで, 酵母, 乳酸菌それぞれ単独で発電を行ったものよりも高い発電力が得られた. また, 酵母培養液に燻炭を添加することにより代謝が促進されることが示され, 燻炭を酵母微生物燃料電池へ組み込むことで高い発電力を得ることができた. [Abstract] Microbial fuel cells (MFCs) are devices that can use microorganisms as biocatalysts to directly convert chemical energy to electricity. Combining with wastewater treatment process with MFCs makes the electricity generation possible from organic waste. In this study, we used yeast and two approaches were attempted to enhance the performances of MFCs. One approach was a mixed-species biofilm in coculture of yeasts and lactic acid bacteria, and the other was addition of biocharwhich enhances microbial metabolic activity. We found the combination of yeast and lactic acid bacterium, which forms a strong mixed-species biofilm, produced more electricity than the monoculture of the microbe. Fermentation of yeasts was stimulated by the presence of biochar and the yeast-based MFCs with biochar produced more electricity than those without biochar.
  • Potential of Agricultural Water and Pesticide Solution as Preharvest Sources of Microbial Contamination for Several Fruits, 北田 康祐, 河本 敬子, 阿野 貴司, 泉 秀実, 近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 34, 34, 27, 33,   2014 09 01 , http://ci.nii.ac.jp/naid/120005735161
    Summary:[要旨] 和歌山県産の果実(カキ、ウンシュウミカン、ウメ)の各圃場を対象に, 農業用水, 農薬溶液および収穫果実の微生物汚染度を測定し, これらの栽培環境接触物が微生物汚染源として青果物に及ぼす影響を調査した. 農業用水に使用した水源の微生物汚染度は, 河川水が谷の湧水より1log程度高かった. さらに, 農業用水に農薬を溶解することで, 微生物数が最大で2log程度増加し, 微生物汚染度の増大が確認された. 農薬散布から3日後に収穫されたカキ果実では, 農業用水および農薬溶液から土壌由来あるいは植物病原性の7菌種が移行したと推定され, 農薬散布から8日後のウンシュウミカン果実(2菌種)および31日後のウメ果実(1菌種)よりも多いことが確認された. いずれのサンプルからも食中毒原因菌(verotoxin-producing Escherichia coli, Salmonella, Listeria monocytogenes)は検出されず, 農業用水および農薬溶液を介した微生物的食品安全危害はないことが示された. [Abstract] Microflora of field environments (agricultural water and pesticide solutions diluted with agricultural water) and several fruits (persimmons, satsuma mandarin, and Japanese apricots) were investigated to determine the potential of the field environment as a preharvest source of microbial contamination for the fruits. With agricultural water, the microbial counts in river water were 1 log higher than those counts in mountain spring water. When pesticide products were dissolved in agricultural water, the microbial counts increased by 2 logs. Seven species of soilborne or phytopathogenic bacteria andmolds detected on persimmon fruit harvested 3 days after pesticide application were also found in the agricultural water and pesticide solution, suggesting that the field environment could be a source of preharvest contamination. In comparison there were fewer microbial species found on satsuma mandarin fruit (2 species) and Japanese apricot fruit (1 species) harvested 8days and 31 days after pesticide application, respectively, that appeared to be transferred from the agricultural water and pesticide solutions. Since foodborne pathogens such as Salmonella, verotoxin-producing Escherichia coli, and Listeria monocytogenes were not detected in any of the fruit and environmental samples, it is assumed that there are no microbialfood safety hazards resulting from cross-contamination with the environment.
  • Construction and improvement of yeast-based microbial fuel cells, 江邉 正平, 大池 達矢, 岡南 政宏, 阿野 貴司, 近畿大学生物理工学部紀要 = Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 34, 34, 15, 26,   2014 09 01 , http://ci.nii.ac.jp/naid/120005735160
    Summary:[要旨] 微生物燃料電池とは, 微生物が有機物を分解する過程で生じる電子を電気エネルギーとして回収する装置である. 微生物燃料電池は, 有機物から直接電気エネルギーを取り出すことが出来ることから, 廃水処理と組み合わせた利用が考えられている. 本研究では, モデル微生物であり, 数多くの食品にも利用されている酵母を用いた微生物燃料電池の開発を行った. そして酵母を用いた微生物燃料電池の発電効率を上げるため2つのアプローチを試みた. それらは酵母と乳酸菌を共培養した際に形成される複合バイオフィルムと微生物燃料電池を組み合わせたものと、燻炭による代謝促進と微生物燃料電池を組み合わせたものである. 本研究により, 強い複合バイオフィルム形成を行う酵母と乳酸菌の組み合わせを特定できた. この組み合わせを微生物燃料電池に組み込むことで, 酵母, 乳酸菌それぞれ単独で発電を行ったものよりも高い発電力が得られた. また, 酵母培養液に燻炭を添加することにより代謝が促進されることが示され, 燻炭を酵母微生物燃料電池へ組み込むことで高い発電力を得ることができた. [Abstract] Microbial fuel cells (MFCs) are devices that can use microorganisms as biocatalysts to directly convert chemical energy to electricity. Combining with wastewater treatment process with MFCs makes the electricity generation possible from organic waste. In this study, we used yeast and two approaches were attempted to enhance the performances of MFCs. One approach was a mixed-species biofilm in coculture of yeasts and lactic acid bacteria, and the other was addition of biocharwhich enhances microbial metabolic activity. We found the combination of yeast and lactic acid bacterium, which forms a strong mixed-species biofilm, produced more electricity than the monoculture of the microbe. Fermentation of yeasts was stimulated by the presence of biochar and the yeast-based MFCs with biochar produced more electricity than those without biochar.
  • 2P-143 Construction and improvement of microbial fuel cells using alkaliphilic bacteria, Ebe Shohei, Ohike Tatsuya, Okanami Masahiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906527
  • 3P-101 Evaluation of Trichoderma atroviride HNT-01 as biocontrol agent, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Yamamoto Shigeki, Hirose Yoichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906783
  • 3P-100 Development of biocontrol agents from actinomycetes, Uetsuki Yoshihiro, Ohike Tatsuya, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906782
  • 3P-099 Approaches to control plant pathogens using bacteria isolated from earthworms, Washio Naoki, Ohike Tatsuya, Okanami Masahiro, Ano Takashi, 日本生物工学会大会講演要旨集, 66,   2014 08 05 , http://ci.nii.ac.jp/naid/110009906781
  • 放線菌を用いた微生物農薬の開発, UETSUKI YOSHIHIRO, OIKE TATSUYA, MATSUKAWA TETSUYA, OKANAMI MASAHIRO, KAJIYAMA SHIN'ICHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 66th, 219,   2014 08 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402242826402429
  • 十和田石粉末から単離したTrichoderma atroviride HNT‐01の微生物農薬としての評価, OIKE TATSUYA, MATSUKAWA TETSUYA, OKANAMI MASAHIRO, KAJIYAMA SHIN'ICHIRO, YAMAMOTO SHIGEKI, HIROSE YOICHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 66th, 220,   2014 08 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402269255755561
  • ミミズより単離した細菌を用いた植物病原菌抑制の試み, WASHIO NAOKI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 66th, 219,   2014 08 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402277487722506
  • アルカリ性条件下における微生物燃料電池の発電力向上の試み, EBE SHOHEI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, 日本生物工学会大会講演要旨集, 66th, 142,   2014 08 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402282174331976
  • Jatropha curcas種子採油残渣を用いた微生物農薬の培養, ISHIDA YUKO, OIKE TATSUYA, MATSUKAWA TETSUYA, OKANAMI MASAHIRO, KAJIYAMA SHIN'ICHIRO, ANO TAKASHI, 日本農芸化学会大会講演要旨集(Web), 2014, 2A14P15 (WEB ONLY),   2014 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402235221867865
  • 低栄養性細菌を用いた微生物農薬候補株の探索, OIKE TATSUYA, MATSUKAWA TETSUYA, OKANAMI MASAHIRO, KAJIYAMA SHIN'ICHIRO, ANO TAKASHI, 日本農芸化学会大会講演要旨集(Web), 2014, 2A14P14 (WEB ONLY),   2014 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402236415833097
  • アルカリ性条件下における微生物燃料電池の構築, EBE SHOHEI, OIKE TATSUYA, OKANAMI MASAHIRO, ANO TAKASHI, 日本農芸化学会大会講演要旨集(Web), 2014, 4A01A11 (WEB ONLY),   2014 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402274931648653
  • 植物と植物生長促進細菌の相互作用に関わる分子メカニズムの解析, OURA MARI, IMAI AKITO, ITO YUYA, OI CHIHIRO, MUKAI KAZUMA, TSUDA KOSUKE, HAZAMA MIKI, ANO TAKASHI, OKANAMI MASAHIRO, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 1P-0477 (WEB ONLY),   2014 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502285218077157
  • 2P-072 Properties of lipases produced by a biodegradable plastic-degrading bacterium, Okanami Masahiro, Kitahara Yasuhisa, Chida Hikari, Ano Takashi, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737738
  • 1P-214 Growth properties of sweet potato in the triangle shelf multi-layer cultivation method and its methane fermentation condition, Suzuki Takahiro, Sakamoto Masaru, Ano Takashi, Shintani Noboru, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737594
  • 1P-206 Production of antifungal lipopeptide iturin A by glycerol as BDF by-product, Ohike Tatsuya, Hashigami Kohei, Ishida Yuko, Matsukawa Tetsuya, Okanami Masahiro, Kajiyama Shin'ichiro, Ano Takashi, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737586
  • 1P-174 Electricity Generation in Microbial Fuel Cells with Co-Cultivation of Lactic Acid Bacteria and Yeasts, Ebe Shouhei, Matsuoka Tomonori, Ohike Tatsuya, Okanami Masahiro, Ano Takashi, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737554
  • 〈Original Papers〉Suppressive activity of fermented soybeans, natto, against phytopathogens, 村田 大地, 澤野 沙耶佳, 大池 達矢, 岡南 政宏, 阿野 貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学生物理工学部紀要, 31, 31, 47, 57,   2013 03 01 , http://ci.nii.ac.jp/naid/120005735148
    Summary:[要旨] 近年、自然界に生息している微生物の拮抗作用に着目した微生物農薬が注目を集めている。微生物農薬への期待が高まる中、用いる微生物の安全性にも留意すべきであると考え、スクリーニング源として、食品である納豆に着目した。市販されている12社、31種の納豆製品について、植物病原菌であるFusarium oxysporumとRhizoctonia solani対する抑制能を調べた。その結果、抑制能力の大小は認められるものの、R. solaniに対しては30種が抑制を示し、F. oxysporumについては28種の納豆が抑制能を示した。そこで、納豆から抑制菌を単離し、その抑制能が抗菌物質の生産性にあることを見出した。特に、強い抑制能を示す菌は、耐熱性の抗菌物質を生産しており、16S rRNA遺伝子配列の解析結果から、Bacillus amyloliquefaciensであることが推定された。長年にわたり食用として消費されている納豆から単離された微生物であるため、人体に対する安全性は非常に高いと考えられる。このため、従来にない安全な微生物農薬候補株であると考えられ、食品由来の微生物農薬の開発が期待される。 [Abstract] Inhibitory activity of a traditional Japanese fermented food, natto, was found against two plant pathogens, Rhizoctonia solani and Fusarium oxysporum. Bacteria which showed inhibition were isolated from 31 products of natto from 12 companies. Two isolated bacteria showed stronger antagonistic effect in vitro against plant pathogens on PDA and TSA media. The supernatant of the bacterial culture also showed strong activity against R. solani, which meant the antimicrobial substances were produced and secreted into the medium. The bacteria, which were indicated as Bacillus amyloliquefaciens from a partial sequence of 16s rRNA gene, did not show the production of y -PGA but showed the production of siderophore.
  • 〈Original Papers〉Suppressive activity of fermented soybeans, natto, against phytopathogens, 村田 大地, 澤野 沙耶佳, 大池 達矢, 岡南 政宏, 阿野 貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 31, 47, 57,   2013 03 , http://ci.nii.ac.jp/naid/120005260078
    Summary:[要旨] 近年、自然界に生息している微生物の拮抗作用に着目した微生物農薬が注目を集めている。微生物農薬への期待が高まる中、用いる微生物の安全性にも留意すべきであると考え、スクリーニング源として、食品である納豆に着目した。市販されている12社、31種の納豆製品について、植物病原菌であるFusarium oxysporumとRhizoctonia solani対する抑制能を調べた。その結果、抑制能力の大小は認められるものの、R. solaniに対しては30種が抑制を示し、F. oxysporumについては28種の納豆が抑制能を示した。そこで、納豆から抑制菌を単離し、その抑制能が抗菌物質の生産性にあることを見出した。特に、強い抑制能を示す菌は、耐熱性の抗菌物質を生産しており、16S rRNA遺伝子配列の解析結果から、Bacillus amyloliquefaciensであることが推定された。長年にわたり食用として消費されている納豆から単離された微生物であるため、人体に対する安全性は非常に高いと考えられる。このため、従来にない安全な微生物農薬候補株であると考えられ、食品由来の微生物農薬の開発が期待される。 [Abstract] Inhibitory activity of a traditional Japanese fermented food, natto, was found against two plant pathogens, Rhizoctonia solani and Fusarium oxysporum. Bacteria which showed inhibition were isolated from 31 products of natto from 12 companies. Two isolated bacteria showed stronger antagonistic effect in vitro against plant pathogens on PDA and TSA media. The supernatant of the bacterial culture also showed strong activity against R. solani, which meant the antimicrobial substances were produced and secreted into the medium. The bacteria, which were indicated as Bacillus amyloliquefaciens from a partial sequence of 16s rRNA gene, did not show the production of y -PGA but showed the production of siderophore.
  • 〈Original Papers〉A core sequence within a transcriptional activation domain of the aryl hydrocarbon receptor AhR, 岡南 政宏, 藤谷 純也, 阿野 貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 28, 1, 11,   2011 09 01 , http://ci.nii.ac.jp/naid/120005735128
    Summary:[要旨] AhR(arylhydrocarbon receptor)は、bHLHPASファミリーに属する受容体型転写因子である。AhRのC末側のおよそ350アミノ酸残基からなる領域は、転写活性化能を担っていることが知られている。我々は、マウスAhRのC末側領域(a.a. 449-805)を酵母転写因子GAL4のDNA結合ドメインとの融合タンパク質として酵母内で発現させると、この融合タンパク質は、GAL4結合配列をもつレポーター遺伝子の転写を強く誘導することを見出した。AhRのC末側領域の転写活性化領域を詳しく解析した結果、61アミノ酸残基からなる転写活性化に必要なコア領域を同定することに成功した。酵母内でもAhRの転写活性化領域が機能することから、酵母からマウスやヒトにも広く存在する普遍的なコファクターが、AhRの転写活性化に関与していると考えられた。そこで、普遍的コファクターであるSAGA/PCAF複合体の構成因子であるSpt3を欠失させた結果、同定した61アミノ酸残基による転写活性化能は著しく失われた。これらのことから、AhRは、in vivoにおいてPCAF複合体を介して転写を活性化していることが示唆された。 [Abstract] Aryl hydrocarbon receptor (AhR) is a ligand-activated, bHLH-PAS-type anscription factor that regulates genes involved in xenobiotic metabolism, cellular proliferation and differentiation. The C-terminal region of mouse AhR (a.a. 449-805) has been shown to have a trans-activating potency to transcription. We found that this region, when expressed as a protein fused to the DNA-binding domain of the yeast transcription factor GAL4 (a.a. 1-147) in yeast cells, strongly activated transcription of a reporter gene driven by GAL4-binding sequences in a ligand-independent manner. Various portions of AhR were investigated for their trans-activating potencies, and subsequently we identified a 61-amino acid-stretch capable of activating transcription. The 61-amino acid-region failed to activate transcription in yeast which lacks Spt3 of the SAGA complex, a possible counterpart of the PCAF complex widely conserved among higher eukaryotic cells. These results suggest that AhR should activate transcription via the PCAF complex in vivo.本研究は近畿大学生物理工学部戦略的研究No.05-II-3, 2006-2008およびNo.08-IV-17, 2009の助成を受けた. Fujitani, Jun-ya
  • 〈Original Papers〉A core sequence within a transcriptional activation domain of the aryl hydrocarbon receptor AhR, 岡南 政宏, 藤谷 純也, 阿野 貴司, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 28, 1, 11,   2011 09 , http://ci.nii.ac.jp/naid/120003460254
    Summary:本研究は近畿大学生物理工学部戦略的研究No.05-II-3, 2006-2008およびNo.08-IV-17, 2009の助成を受けた. Fujitani, Jun-ya[要旨] AhR(arylhydrocarbon receptor)は、bHLHPASファミリーに属する受容体型転写因子である。AhRのC末側のおよそ350アミノ酸残基からなる領域は、転写活性化能を担っていることが知られている。我々は、マウスAhRのC末側領域(a.a. 449-805)を酵母転写因子GAL4のDNA結合ドメインとの融合タンパク質として酵母内で発現させると、この融合タンパク質は、GAL4結合配列をもつレポーター遺伝子の転写を強く誘導することを見出した。AhRのC末側領域の転写活性化領域を詳しく解析した結果、61アミノ酸残基からなる転写活性化に必要なコア領域を同定することに成功した。酵母内でもAhRの転写活性化領域が機能することから、酵母からマウスやヒトにも広く存在する普遍的なコファクターが、AhRの転写活性化に関与していると考えられた。そこで、普遍的コファクターであるSAGA/PCAF複合体の構成因子であるSpt3を欠失させた結果、同定した61アミノ酸残基による転写活性化能は著しく失われた。これらのことから、AhRは、in vivoにおいてPCAF複合体を介して転写を活性化していることが示唆された。 [Abstract] Aryl hydrocarbon receptor (AhR) is a ligand-activated, bHLH-PAS-type anscription factor that regulates genes involved in xenobiotic metabolism, cellular proliferation and differentiation. The C-terminal region of mouse AhR (a.a. 449-805) has been shown to have a trans-activating potency to transcription. We found that this region, when expressed as a protein fused to the DNA-binding domain of the yeast transcription factor GAL4 (a.a. 1-147) in yeast cells, strongly activated transcription of a reporter gene driven by GAL4-binding sequences in a ligand-independent manner. Various portions of AhR were investigated for their trans-activating potencies, and subsequently we identified a 61-amino acid-stretch capable of activating transcription. The 61-amino acid-region failed to activate transcription in yeast which lacks Spt3 of the SAGA complex, a possible counterpart of the PCAF complex widely conserved among higher eukaryotic cells. These results suggest that AhR should activate transcription via the PCAF complex in vivo.
  • Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima., 大池 達矢, 松川 哲也, 岡南 政宏, 阿野 貴司, 梶山 慎一郎, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, 27, 29, 37,   2011 03 01 , http://ci.nii.ac.jp/naid/120005735121
    Summary:[要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60%の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60%) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
  • Distributional Analysis of Phorbol Esters in J. curcas and J. integerrima., 大池 達矢, 松川 哲也, 岡南 政宏, 阿野 貴司, 梶山 慎一郎, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, 27, 29, 37,   2011 03 , http://ci.nii.ac.jp/naid/120003002881
    Summary:[要旨] トウダイグサ科の落葉低木であるJatropha curcasは、種子の仁に40-60%の中性脂質を含んでおり、乾燥地域や貧栄養土壌などの荒廃地でも生育可能であるため、現在、東南アジア諸国で使用され、バイオディーゼル燃料(BDF)となる資源として注目されている。しかし、J. curcasの種子中からは、発がんプロモーション活性物質であるフォルボールエステル類が検出されており、BDF生産者の健康や環境への影響が懸念されている。その一方で、Jatrophaは、観賞用植物としても親しまれており、街路樹に利用されるなど現在でも多くの種が流通している。しかしながら、観賞用のJatrophaは、BDF用のJatrophaに比べると商業的な価値が低いため、種子や各器官に関する基礎的な知見が乏しい。そこで本研究では、Jatropha integerrimaにおけるフォルボールエステル類の分布ををJatropha curcasを元に、種子油の脂肪酸組成分析を行なった。その結果、Jatropha integerrimaの種子および葉においてフォルボールエステル類の存在が見出され、種子油に含まれる脂肪酸の組成は、両植物では大きく異なることが分かった。本研究の結果は、観賞用Jatrophaにおけるフォルボールエステル類のリスク評価と、BDF植物の優良品種開発に寄与すると期待される。 [Abstract]Jatropha curcas, a member of Euphorbiaceae family, has recently been attracted the considerable attention as a potential source of bio-diesel due to the high adaptbility to arid and oligotrophic soil and oil concern (40-60%) in seed kernel. The seed, however, contains toxic compounds including phorbol esters, well known as a tumor promoting agent, and thus influence of these compounds on environment and people engaged in the production should be concerned. In contrast, I integerrima, widely distributed for ornamental use, has not been focused, and little is know about phorbol esters contained in this species. In this study, we performed phorbol esters analysis and comparison of fatty acid composition between J. curcas and I integerrima. Phorbol esters were detected in J. integerrima seeds and leaves as well as J. curcas, while profiles of fatty acids were different among these species.
  • Experimental investigation of effect of low-energy pulsed atmospheric electron beam on bacterial cells, Hamid Ghomi, Hamid Ghomi, Mohammad, Shahedur Rahman, Priya R. Chalise, Yasushi Hayashi, Masato Watanabe, Akitoshi Okino, Takashi Ano, Makoto Shoda, Eiki Hotta, Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, 44, 12, 8698, 8701,   2005 12 08 , 10.1143/JJAP.44.8698, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=31544442455&origin=inward
    Summary:In this paper, the effect of a pulsed low-energy electron beam on bacteria has been explored. The experiment has been carried out using an electron beam with a pulse duration of 5 μs and an acceleration voltage of 80 kV. It is shown that homogeneous irradiation with an electron beam decontaminates surfaces. Completely Sterilization is achieved at a low concentration of bacteria. With increasing concentration of bacteria or for a wet target, the survivability of a microorganism increases. A spectroscopic experiment and also scanning electron microscopy (SEM) images show that the low-energy electron beam does not break the outer structure of a bacterial cell and that bacteria inactivation occurs through internal chemical or genetic changes. © 2005 The Japan Society of Applied Physics.
  • 3E09-5 Induced germination improved the production of iturin A by Bacillus subtilis RB14 :, RAHMAN Mohammad Shahedur, ANO Takashi, SHODA Makoto, 日本生物工学会大会講演要旨集, 17,   2005 , http://ci.nii.ac.jp/naid/110007321892
  • APPLICATION OF SECONDARY EMISSION ELECTRON GUN ON BACTERIAL INACTIVATION, CHALISE Priya R., RAHMAN Mohammad S., GHOMI Hamid, WATANABE Masato, OKINO Akitoshi, ANO Takashi, SHODA Makoto, HOTTA Eiki, 2003, 72, 13, 16,   2003 12 15 , http://ci.nii.ac.jp/naid/10018979875
  • 801 Suppressive effect of Bacillus subtilis culture broth containing high concentration of Iturin A on damping-off of tomato caused by Rhizoctonia solani, Mizumoto Shinji, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 14,   2002 09 25 , http://ci.nii.ac.jp/naid/110007320198
  • Ammonium removal characteristics by immobilized Alcaligenes faecalis No.4, Wako Ryohei, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 13,   2001 08 24 , http://ci.nii.ac.jp/naid/110002922759
  • Effect of high magnetic field on growth of Escherichia coli-Dependence of NaCl concentration, Horiuchi Shin-ichiro, Isizaki Yoshimasa, Okuno Kazumasa, Ano Takasi, Shoda Makoto, 日本生物工学会大会講演要旨集, 13,   2001 08 24 , http://ci.nii.ac.jp/naid/110002923146
  • Expression of chitinase in Bacillus subtilis and its application to biocontrol, Kuraya Ayumi, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 13,   2001 08 24 , http://ci.nii.ac.jp/naid/110002923277
  • Heterotrophic nitrification and denitrification by Alcaligenes faecalis No.4, Wako Ryohei, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 12,   2000 07 10 , http://ci.nii.ac.jp/naid/110002922157
  • Conditions to suppress death rate of Escherichia coli under high magnetic field., Horiuchi Shin-ichiro, Ishizaki Yoshimasa, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 12,   2000 07 10 , http://ci.nii.ac.jp/naid/110002922472
  • Physiological change in death-suppressed Escherichia coli under high magmetic field., Ishizaki Yoshimasa, Horiuchi Shin-ichiro, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 12,   2000 07 10 , http://ci.nii.ac.jp/naid/110002922473
  • High magnetic field enhances rpoS transcription of Escherichia coli in stationary phase., Tsuchiya Koji, Okuno Kazumasa, Ano Takashi, Tanak Kan, Takahashi Hideo, Shoda Makoto, 日本生物工学会大会講演要旨集, 11,   1999 08 16 , http://ci.nii.ac.jp/naid/110002921104
  • Effect of High Magnetic Field on GASP (Growth Advantage in Stationary Phase), Fujinami Ryo, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 11,   1999 08 16 , http://ci.nii.ac.jp/naid/110002921105
  • Control of Tomato Damping-off Caused by Rhizoctonia solani by the Heterotrophic Nitrifler Alcaligenes faecafis No.4., Honda Naohiro, Hirai Mituyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 11, 2, 153, 162,   1999 08 16 , 10.3186/jjphytopath.65.153, http://ci.nii.ac.jp/naid/110002921294
  • Integrated effect of Bacillus subtilis RB14-C and flutolanil on suppression of damping-off of tomato caused by Rhizoctonia solani, Kondo Maki, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 11,   1999 08 16 , http://ci.nii.ac.jp/naid/110002921295
  • Control of Tomato Damping-off Caused by Rhizoctonia solani by the Heterotrophic Nitrifier Alcaligenes faecalis and Its Product, Hydroxylamine, H0NDA Naohiro, HIRAI Mitsuyo, ANO Takashi, SH0DA Makoto, Annals of the Phytopathological Society of Japan, 65, 2, 153, 162,   1999 04 25 , 10.3186/jjphytopath.65.153, http://ci.nii.ac.jp/naid/110002733121
    Summary:Alcaligenes faecalis No. 4 (No. 4), which showed a significant suppressive effect on the growth of 13kinds of plant pathogens in vitro, produced hydroxylamine, nitrite and nitrate in liquid cultivation. This indicates that No. 4 is a heterotrophic nitrifier. As hydroxylamine solution formed a clear growth inhibitory zone on a plate inoculated with Rhizoctonia solani K-l in vitro, the aforementioned suppressive effect was considered to be due to the hydroxylamine produced by No. 4 during heterotrophic nitrification. When culture broth, cell suspension and supernatant of No. 4 were applied to sterilized soil containing R. solani, the damping-off of tomato seedlings caused by R. solani was suppressed significantly. However, in non-sterilized soil, damping-off was suppressed only by the culture broth and the cell suspension treatments, presumably because hydroxylamine in the supernatant was decomposed in soil. Transposon mutagenesis was applied to No. 4 to produce a non-hydroxylamine-producing mutant, No. 4-1, which showed no suppressive effect on the growth of R. solani either in vitro or in vivo. These data indicate that the hydroxylamine produced by No. 4 is involved in the suppression of damping-off caused by R. solani.
  • Control of Tomato Damping-off Caused by Rhizoctonia solani by the Heterotrophic Nitrifier Alcaligenes faecalis and Its Product, Hydroxylamine., HONDA Naohiro, HIRAI Mitsuyo, ANO Takashi, SHODA Makoto, Jpn. J. Phytopathol., 65, 2, 153, 162,   1999 , 10.3186/jjphytopath.65.153, http://ci.nii.ac.jp/naid/130003914408
    Summary:Alcaligenes faecalis No.4 (No.4), which showed a significant suppressive effect on the growth of 13 kinds of plant pathogens in vitro, produced hydroxylamine, nitrite and nitrate in liquid cultivation. This indicates that No.4 is a heterotrophic nitrifier. As hydroxylamine solution formed a clear growth inhibitory zone on a plate inoculated with Rhizoctonia solani K-1 in vitro, the aforementioned suppressive effect was considered to be due to the hydroxylamine produced by No.4 during heterotrophic nitrification. When culture broth, cell suspension and supernatant of No.4 were applied to sterilized soil containing R. solani, the damping-off of tomato seedlings caused by R. solani was suppressed significantly. However, in non-sterilized soil, damping-off was suppressed only by the culture broth and the cell suspension treatments, presumably because hydroxylamine in the supernatant was decomposed in soil. Transposon mutagenesis was applied to No.4 to produce a non-hydroxylamine-producing mutant, No.4-1, which showed no suppressive effect on the growth of R. solani either in vitro or in vivo. These data indicate that the hydroxylamine produced by No.4 is involved in the suppression of damping-off caused by R. solani.
  • Effect of pleiotropic regulatory gene degQ on plipastatin production by Bacillus subtilis YB8., Tsuge Kenji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 10,   1998 08 31 , http://ci.nii.ac.jp/naid/110002921800
  • (50) Biological Control of Rhizoctonia solani Damping-off of Tomato with Alcaligenes faecalis No.4, Honda N, Hirai M, Ano T, Shoda M, Annals of the Phytopathological Society of Japan, 64, 4, 339, 340,   1998 08 25 , http://ci.nii.ac.jp/naid/110002732456
  • Characteristics of the supernatant of E.coli cultivated under high magnetic field, Ezawa Kota, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 9,   1997 09 05 , http://ci.nii.ac.jp/naid/110002924577
  • Analysis of a gene cluster responsible for plipastatin biosynthesis, Tsuge Kenji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 9,   1997 09 05 , http://ci.nii.ac.jp/naid/110002924300
  • Analysis of a gene cluster responsible for iturin A production in Bacillus subtilis RB14, Nishiguchi Aki, Tsuge Kenji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 9,   1997 09 05 , http://ci.nii.ac.jp/naid/110002924301
  • Biocontrol of Rhizoctonia solani damping-off of Tomato with A.faecalis No.4, Honda Naohiro, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 9,   1997 09 05 , http://ci.nii.ac.jp/naid/110002924743
  • Effect of homogeneous and inhomogeneous high magnetic fields on bacterial growth measured under a newly developed superconducting magnet biosystem, Makoto Shoda, Kazuhiro Nakamura, Kazumasa Okuno, Takashi Ano, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, 5, 1867, 1868,   1996 12 01 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0030314135&origin=inward
    Summary:A new superconducting magnet biosystem (SBS) was developed which can provide 0.5 to 7 tesla(T) magnetic fields. The aerobic growth of a bacterium, Bacillus subtilis MI113 was investigated under homogeneous 7 T and inhomogeneous 5.2-6.1 T magnetic fields in SBS. In the stationary phase, the cell number under magnetic field was about 2 times higher than that of a control, indicating that the high magnetic field inhibits the death rate of bacterial cells. A genetically transformed B. subtilis MI113(pC112) produced higher concentration of a lipopeptide antibiotic, surfactin under high magnetic field, reflecting higher cell number under high magnetic field compared with that in geomagnetic field.
  • Analysis of a Bacillus subtilis gene responsible for the regulation of surfactin production, Ohta Osamu, Tsuge Kenji, Huang Cheih-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 8,   1996 09 05 , http://ci.nii.ac.jp/naid/110002925046
  • Biocontrol by Bacillus subtilis RB14-C, against a phytopathogen of tomato., Kurosu Kazuhiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 8,   1996 09 05 , http://ci.nii.ac.jp/naid/110002925319
  • Carboxyl-terminal residues of genes homologous to Ipa involved in the production of lipopeptidic secondary metabolities., Huang Chieh-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 8,   1996 09 05 , http://ci.nii.ac.jp/naid/110002925328
  • Isolation and characterization of a new gene which enhances surfactin production in Bacillus subtilis RB14., Ishiguro Yutaka, Huang Chieh-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 8,   1996 09 05 , http://ci.nii.ac.jp/naid/110002925329
  • Effect of magnetic fields on the logarithmic growth pahse of E. coli, Tsuchiya Koji, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 7,   1995 09 15 , http://ci.nii.ac.jp/naid/110002947909
  • A study of electric field homogeneity of CHEF (C__-ontour-clamped H__-omogeneous E__-lectric F__-ield) electrophoresis apparatus., Tsuge Kenji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 7,   1995 09 15 , http://ci.nii.ac.jp/naid/110002947911
  • Multicopy effect of sfp^0 in the production of lipopeptide antibiotics of Bacillus subtilis., Huang Cheih-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 7,   1995 09 15 , http://ci.nii.ac.jp/naid/110002947952
  • Isolation and characterization of a mutant Bacillus subtilis which showed suppressibility at 15℃., Kosaka Takafumi, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 7,   1995 09 15 , http://ci.nii.ac.jp/naid/110002948006
  • Suppression of plant pathogens by A-faecalis No.4, Honda Naohiro, Hirai Mitsuyo, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 7,   1995 09 15 , http://ci.nii.ac.jp/naid/110002948007
  • Survival of Bacillus subtilis NB22 and its transformant in soil, Yukinori Tokuda, Takashi Ano, Makoto Shoda, Applied Soil Ecology, 2, 85, 94,   1995 01 01 , 10.1016/0929-1393(94)00042-6, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0001808751&origin=inward
    Summary:Bacillus subtilis NB22 is an anti fungal-antibiotic (iturin) producer that expresses broad suppressibility of phytopathogenic fungi and bacteriaand is considered as a potential biological pesticide. The survival of B. subtilis NB22-1, which is a spontaneous streptomycin-resistant mutant of NB22 and its transformants with plasmid DNA, was investigated in soil, The number of vegetative cells of the host strain NB22-1 declined initially in soil at 15°C, but the population was stabilized at the level of spores which were formed during incubation in soil. Neither nutritional amendment nor the raising of temperature were effective for the higher induction of spore formation in soil, However, simultaneous treatment of increasing the temperature and nutritional amendment was effective for the rapid induction of spores in the soil, which led to stabilization of the population at the level of 107 spores g-1 dry soil of the initially added cell number. Vegetative cells of the transformants NB22-1 (pC 194) and NB22-1(pUB1 10) also sporulated efficiently in soil under these conditions and were stable for 50 days, but died out within 10 days without such treatment. The stability of plasmid pUB110 was high (around 90%), but pC194 was quickly cured and its stability declined to about 50%. The stability of the two plasmids was similar to that in liquid culture using sporulation medium. When spores of NB22-1 or its transformants were introduced into the soil, they persisted at high level and showed no loss in their viability until the end of the experiment at 50 days. © 1995.
  • Detection of plasmid transfer from Escherichia coli to Baciilus subtilis by a shuttle vector, Ano Takashi, Huang Chieh-Chen, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923368
  • Production of biosurfactant surfactin with dried Okara in solid state fermentation, Nakayama Satoshi, Ohno Akihiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923540
  • Instability of pC194, a vector plasmid of Bacillus subtilis during sporulation., Asaka Orie, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923647
  • Cloning of a gene responsible for an antibiotic production from Bacillus subtilis YB8., Tsuge Kenji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923648
  • Effect of high magnetic field to the culture of Bacillus subtilis, Okuno Kazumasa, Nakamura Kazuhiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923760
  • Effect of high magnetic field on the growth of E. coli., Tsuchiya Koji, Okuno Kazumasa, Nakamura Kazuhiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 6,   1994 10 10 , http://ci.nii.ac.jp/naid/110002923761
  • Bacterial Cultivation in high magnetic fields by 7-tesla superconducting magnet, TAKASHI ANO, TEION KOGAKU, 29, 12, 646, 652,   1994 , 10.2221/jcsj.29.646, http://ci.nii.ac.jp/naid/130003672909
    Summary:A new superconducting magnet system for bacterial cultivation was developed. The superconducting magnet has a horizontal room-temperature bore with a diameter of 160mm, and provides a homogeneous magnetic field of 7T±0.5% for a 200-mm-long by 100-mm-diameter region. This homogeneous field region contains an incubator, where bacteria are cultivated aerobically at 10 to 70°C±0.1°C while being shaken. The culture exposed to the high magnetic field is compared with a control culture incubated at below geomagnetic field strength. Cultivation of Escherichia coli was carried out both in homogeneous and in inhomogeneous fields, and 1.4-3.6 times the number of viable cells of the control culture was observed in a stationary phase.
  • Basic analysis of Bacillus subtilis NB22 and its application to biological control., M. Shoda, T. Ano, Bioprocess technology, 19, 641, 664,   1994 01 01 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0028310575&origin=inward
  • Nucleotide Sequence and Characteristics of a Gene. lpa-14 Responsible for the Biosynthesis of the Lipopeptide Antibiotics Iturin A and Surfactin from B. sobtills RB14, Huang Chieh-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 5,   1993 11 10 , http://ci.nii.ac.jp/naid/110002947369
  • Production of surfactin by solid state fermentation with a recombinant B. subtilis MI113(pC112), Ohno Akihiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 5,   1993 11 10 , http://ci.nii.ac.jp/naid/110002947418
  • Stability of Bacillus subtills NB22-1 and its transformants in soil and in liquid culture., Tokuda Yukinori, Sekiguchi Yoshihito, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 5,   1993 11 10 , http://ci.nii.ac.jp/naid/110002947545
  • Characteristics of Bacillus subtilis RB14 which produces antifungal antibiotic iturin, Asaka Orie, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 4,   1992 10 10 , http://ci.nii.ac.jp/naid/110002923844
  • Tetracycline induced gene amplification on the chromosome of Bacillus subtilis 168, Huang Chieh-Chen, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 4,   1992 10 10 , http://ci.nii.ac.jp/naid/110002923846
  • Molecular cloning of a gene responsible for the biosynthesis of the lipopeptide antibiotics iturin and surfactin., Hiraoka Hideji, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 4, 5, 323, 326,   1992 10 10 , 10.1016/0922-338X(92)90068-6, http://ci.nii.ac.jp/naid/110002923888
    Summary:A gene responsible for the production of iturin and surfactin was cloned and analyzed in the original wild strain Bacillus subtilis RB14, a coproducer of surfactin and iturin, as well as in B. subtilis MI113, a derivative of strain B. subtilis 168. The cloned gene lpa (lipopeptide antibiotic production) permitted only surfactin production in strain MI113, but when it was destroyed in RB14, the defective strain simultaneously lost the ability to produce both surfactin and iturin. The ability was restored by the introduction of the intact lpa gene into the strain. © 1992.
  • Production of Antifungal Peptide Antibiotics Iturin by Bacillus subtilis NB22 in Solid State Fermentation, Ohno Akihiro, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 4,   1992 10 10 , http://ci.nii.ac.jp/naid/110002924049
  • Stability of transformed Bacillus subtilis NB22 with plasmids in solid medium and in soil., TOKUDA YUKINORI, ANO TAKASHI, SHODA MAKOTO, 日本生物工学会大会講演要旨集, 4,   1992 10 10 , http://ci.nii.ac.jp/naid/110002924099
  • Response of Escherichia coli to Electric Pulse Imposition, Tsuchiya Koji, Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本生物工学会大会講演要旨集, 4,   1992 10 10 , http://ci.nii.ac.jp/naid/110002924229
  • Ultra-rapid Transformation of Escherichia coil by an Alkali Cation, ANO Takashi, SHODA Makoto, Bioscience, biotechnology, and biochemistry, 56, 9, 1505,   1992 09 23 , 10.1271/bbb.56.1505, http://ci.nii.ac.jp/naid/110002691839
  • Cloning of a Gene Responsible For The Specific Production of An Antifungal Antibiotic Iturin With n-C,6-β-Amino Acid Residue, Yuko Matsuno, Takashi Ano, Makoto Shoda, Journal of General and Applied Microbiology, 38, 5, 505, 509,   1992 , 10.2323/jgam.38.505, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027069461&origin=inward
  • Suppressive Effect In Vitro of Magnesium L-Ascorbyl-2-phosphate (APM), an Ascorbic Acid Derivative, on the Impairment of DNA by Glucose-6-phosphate(Food & Nutrition), ANO Takashi, OHNO Akihiro, SHODA Makoto, Agricultural and biological chemistry, 55, 12, 2967, 2970,   1991 12 23 , 10.1271/bbb1961.55.2967, http://ci.nii.ac.jp/naid/110006325317
    Summary:We attempted to find compounds that suppress the DNA impairment caused by D-glucose-6-phosphate (Glc-6-P) by observing the loss of ability of pBR322 to transform Escherichia coli, and found that APM (magnesium L-ascorbyl-2-phosphate) suppressed the loss of transformability to less than 1/100 of that in the absence of APM. When 2'-deoxyguanosine 5'-monophosphate (dGMP) was incubated in the dark with Glc-6-P and APM, changes in their absorbance patterns were observed, indicating possible suppression by APM of the interactions between dGMP and Glc-6-P. APM weakly suppressed the Maillard reaction.
  • Stability of transformed Bacillus subtillis NB22 in liquid medium and in soil., TOKUDA YUKINORI, ANO TAKASHI, SHODA MAKOTO, 日本醗酵工学会大会講演要旨集, 3,   1991 10 10 , http://ci.nii.ac.jp/naid/110002898574
  • Effect of amino acids on growth rate of E__-. coil under high magnetic field., Okuno Kazumasa, Ano Takashi, Shoda Makoto, 日本醗酵工学会大会講演要旨集, 3,   1991 10 10 , http://ci.nii.ac.jp/naid/110002898735
  • Ultra-rapid transformation of E. coil by alkali cation, Ano Takashi, Shoda Makoto, 日本醗酵工学会大会講演要旨集, 3,   1991 10 10 , http://ci.nii.ac.jp/naid/110002898736
  • High-efficiency transformation of ___- ___- NB22 by electroporation., Matsuno Yuko, Ano Takashi, Shoda Makoto, 日本醗酵工学会大会講演要旨集, 2,   1990 10 10 , http://ci.nii.ac.jp/naid/110002904216
  • Rapid transformation of B___-. ___- by alkali cation treatment., Hiraoka Hideji, Ano Takashi, Shoda Makoto, 日本醗酵工学会大会講演要旨集, 2,   1990 10 10 , http://ci.nii.ac.jp/naid/110002904217
  • Plasmid transformation in Bacillus subtilis NB22, an antifungal-antibiotic iturin producer, Yuko Matsuno, Hideji Hiraoka, Takashi Ano, Makoto Shoda, FEMS Microbiology Letters, 67, 227, 229,   1990 01 15 , 10.1016/0378-1097(90)90200-A, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025096989&origin=inward
    Summary:A transformation system with plasmids was developed for Bacillus subtilis NB22, an antibiotic iturin producing strain. Treatment of B. subtilis NB22 with 4 M KCl was effective for the induction of competence, followed by uptake of plasmid DNA in the presence of polyethylene glycol. The efficiency of transformation of this bacterium with pC194 and pUB110 was 4.1 × 103 and 1.5 × 103 transformants per μg DNA, respectively and the transformation frequency was 3.3 × 10-3 and 7.2 × 10-4, transformants per viable cell, respectively. This method was much faster and three orders of magnitude more efficient in transformation efficiency than protoplast transformation methods. © 1990.
  • Host-Vector System in Iturin-Producing Bacteria, Bacillus subtilis NB22, Matsuno Yuko, Ano Takashi, Shoda Makoto, 日本醗酵工学会大会講演要旨集, 0,   1989 10 11 , http://ci.nii.ac.jp/naid/110002898353
  • DNA sequence studies on replication origin regions of pUB110 and pTB913, Ano Takashi, Imanaka Tadayuki, Aiba Shuichi, 日本醗酵工学会大会講演要旨集, 60,   1985 10 15 , http://ci.nii.ac.jp/naid/110002901761
  • De novo synthesis of poly dAT by the cell extract of a thermophile Bacillus stearothermophilus carrying a plasmid pTB913, Ano Takashi, Imanaka Tadayuki, Aiba Shuichi, 日本醗酵工学会大会講演要旨集, 59,   1984 11 18 , http://ci.nii.ac.jp/naid/110002901478
  • Two replication determinants of an antibiotic-resistance plasmid pTB19, from a thermophilic bacillus, T. Imanaka, T. Ano, M. Fujii, S. Aiba, Journal of General Microbiology, 130, 1399, 1408,   1984 01 01 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021266143&origin=inward
  • Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168, Mohammad Shahedur Rahman, Takashi Ano, Makoto Shoda, JOURNAL OF BIOTECHNOLOGY, 127, 3, 503, 507,   2007 01 , 10.1016/j.jbiotec.2006.07.013, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34447643075&origin=inward
    Summary:Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd 16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C. (c) 2006 Elsevier B.V. All rights reserved.
  • Second stage production of iturin A by induced germination of Bacillus subtilis RB14, Mohammad Shahedur Rahman, Takashi Ano, Makoto Shoda, JOURNAL OF BIOTECHNOLOGY, 125, 4, 513, 515,   2006 10 , 10.1016/j.jbiotec.2006.03.016, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33748324608&origin=inward
    Summary:Bacillus subtilis RB14, a dual producer of lipopeptide antibiotics iturin A and surfactin undergoes sporulation in the submerged fermentation and the production of these secondary metabolites becomes halted. In this study, production of lipopeptide antibiotics was investigated by induced germination of the spores by heat-activation and nutrient supplementation. The induced spores became metabolically active vegetative state and produced lipopeptide antibiotic iturin A that added up the total production at the end of the fermentation. However, additional production of surfactin was not observed. This second time iturin A production by the germinated cells from the spores was defined as second stage production. (c) 2006 Elsevier B.V. All rights reserved.
  • Inactivation of cells and spores of Bacillus subtilis using low energy pulsed electron beam, Mohammad Shahedur Rahman, Hamid Ghomi, Priya Raj Chalise, Yasushi Hayashi, Masato Watanabe, Akitoshi Okino, Takashi Ano, Makoto Shoda, Eiki Hotta, JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 45, 33-36, L881, L883,   2006 09 , 10.1143/JJAP.45.L881, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33748776606&origin=inward
    Summary:In this work, we. present inactivation kinetics of cells and spores of Gram-positive bacteria, Bacillus subtilis following treatment with a high-power and low-energy pulsed electron beam. We observed a high-degree of bacterial inactivation in both the vegetative cells and spores, when they were subjected to the electron beam of energies 60 to 90keV at the beam current density of 28 mA/cm(2). Interestingly, the survival characteristics showed almost a similar response in both the cases as they are plotted against the energy of the electron beam. We believe that our experimental result is one of the first step towards utilizing this technique a a potential tool for inactivation of spores, which is very much demanding in food industries and biomedical field.
  • Experimental investigation of effect of low-energy pulsed atmospheric electron beam on bacterial cells, H Ghomi, MS Rahman, PR Chalise, Y Hayashi, M Watanabe, A Okino, T Ano, M Shoda, E Hotta, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 44, 12, 8698, 8701,   2005 12 , http://ci.nii.ac.jp/naid/10016959679
    Summary:In this paper, the effect of a pulsed low-energy electron beam on bacteria has been explored. The experiment has been carried out using an electron beam with a pulse duration of 5 mu s and an acceleration voltage of 80 kV. It is shown that homogeneous irradiation with an electron beam decontaminates surfaces. Completely Sterilization is achieved at a low concentration of bacteria. With increasing concentration of bacteria or for a wet target, the survivability of a microorganism increases. A spectroscopic experiment and also scanning electron microscopy (SEM) images show that the low-energy electron beam does not break the outer structure of a bacterial cell and that bacteria inactivation occurs through internal chemical or genetic changes.
  • Horizontal transfer of iturin A operon, itu, to Bacillus subtilis 168 and conversion into an iturin A producer, K Tsuge, S Inoue, T Ano, M Itaya, M Shoda, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 49, 11, 4641, 4648,   2005 11 , 10.1128/AAC.49.11.4641-4648.2005, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=27644512734&origin=inward
    Summary:Iturin A and its derivatives are lipopeptide antibiotics produced by Bacillus subtilis and several closely related bacteria. Three iturin group operons (i.e., iturin A, mycosubtilin, and bacillomycin D) of those antibiotic-producing strains have been cloned and sequenced thus far, strongly implying the horizontal transfer of these operons. To examine the nature of such horizontal transfer in terms of antibiotic production, a 42-kb region of the B. subtilis RB14 genome, which contains a complete 38-kb iturin A operon, was transferred via competent cell transformation to the genome of a non-iturin A producer, B. subtilis 168, using a method based on double-crossover homologous recombination with two short landing pad sequences (LPSs) in the genome. The recombinant was positively selected by confirming the elimination of the cI repressor gene, which was localized between the two LPSs and substituted by the transferred segment. The iturin A operon-transferred strain 168 was then converted into an iturin A producer by the introduction of an sfp gene, which encodes 4'-phosphopantetheinyl transferase and is mutated in strain 168. By inserting the pleiotropic regulator degQ, the productivity of iturin A increased sevenfold and was restored to about half that of the donor strain RB14, without the transfer of additional genes, such as regulatory or self-resistance genes.
  • Bacterial inactivation using low-energy pulsed-electron beam, PR Chalise, MS Rahman, H Ghomi, Y Hayashi, M Watanabe, A Okino, T Ano, M Shoda, E Hotta, IEEE TRANSACTIONS ON PLASMA SCIENCE, 32, 4, 1532, 1539,   2004 08 , 10.1109/TPS.2004.830967, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=7244255754&origin=inward
    Summary:In this paper, we report experimental results aimed at the quantitative description of bacterial inactivation using a low-energy (<100 W) pulsed-electron beam. The initial step was to demonstrate the feasibility of a secondary emission electron gun (SEEG) on the area of sterilization/decontamination, which is quantitatively related to the survival characteristics of bacteria. The survival characteristic of most common type of bacterium Escherichia coli JM 109 (E. coli) was studied in an atmospheric pressure decontamination chamber under the increased gun volt age as well as varied pulsed-electron-beam parameters such as current density, pulse width, and repetition rate. A complete inactivation of E. coli was achieved by a single-electron-beam pulse at an accelerating gun voltage of 85 kV in time duration of 5 mus or by five electron beam pulses of the same time duration at a voltage of 77 kV. Several inherent advantages including an efficient bacterial inactivation have been provided as a basis for utilization of SEEG (or low - energy electron beam in general) as a decontamination tool in various biological and medical applications.
  • High-power electron beam source for NOx treatment and bacterial inactivation, Priya Raj Chalise Yashushi Hayashi Masato Watanabe Akitoshi, Okino Mohammad, S. Rahman, Takashi Ano, Makoto, Shoda Eiki Hotta, 4th International Symposium on Non Thermal Plasma Technology for Pollution Control and Sustainable Energy Development,   2004
  • Change in broth culture is associated with significant suppression of Escherichia coli death under high magnetic field, Shin-ichiro Horiuchi, Yoshimasa Ishizaki, Kazumasa Okuno, Takashi Ano, Makoto Shoda, Bioelectrochemistry, 57, 2, 139, 144,   2002 , 10.1016/S1567-5394(02)00111-1, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036021047&origin=inward
    Summary:When Escherichia coli B was cultivated under an inhomogeneous magnetic field of 5.2-6.1 T, a significant 100,000-fold suppression of cell death was observed [Bioelectrochemistry 53 (2001) 149]. The limited magnetic field exposure for 12 h after logarithmic growth phase was sufficient to observe similar suppressive effects on cell death [Bioelectrochemistry 54 (2001) 101]. These results suggest some possible changes in either the medium or the cells during the magnetic field exposure. When the cell-free filtrate of the broth cultured under the magnetic field for 10 h and the cells of E. coli cultivated under the geomagnetic field for 30 h were mixed, and the mixture was subsequently cultivated under the geomagnetic field, the number of cells observed in the filtrate exposed to the high magnetic field was 20,000 times higher than that in the filtrate exposed to the geomagnetic field. When the cells cultivated under the magnetic field for 10 h and the cell-free filtrate of the broth culture exposed to the geomagnetic field were mixed, only a 50-fold difference in the number of cell between under the magnetic field and under the geomagnetic field was observed. This suggests that the filtrate of the broth culture exposed to the magnetic field is primarily responsible for the cell death suppression. It was also revealed that the small difference in pH of the filtrates of the broth culture between under the magnetic field and under the geomagnetic field was critical for the cell death suppression. Copyright © 2002 Elsevier Science B.V.
  • Twelve hours exposure to inhomogeneous high magnetic field after logarithmic growth phase is sufficient for drastic suppression of Escherichia coli death, Y Ishizaki, S Horiuchi, K Okuno, T Ano, M Shoda, BIOELECTROCHEMISTRY, 54, 2, 101, 105,   2001 11 , 10.1016/S1567-5394(01)00108-6, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034768347&origin=inward
    Summary:When Escherichia coli B was aerobically grown at 43 C in a medium whose concentration was one-fourth that of the Luria-Bertani (LB) medium supplemented with 1.5 g/l of glutamic acid, drastic cell death was observed after the end of the logarithmic growth phase. However, when the same experiment was conducted under inhomogeneous 5.2-6.1 T magnetic field, cell death was extremely suppressed and the ratio of viable cell number under high magnetic field to that under geomagnetic field reached as much as 100,000. When the magnetic field exposure was restricted to 12 h after the logarithmic growth phase, a similar high degree of suppressive effect on the death was observed. The findings that the amount of sigma S protein encoded by the rpoS gene under the high magnetic field was larger than that under the geomagnetic field, and that the magnetic field effect disappeared when the rpoS gene-deficient strain was cultivated under the high magnetic field, suggest the interaction of magnetic field with a stationary phase specific gene. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Drastic high magnetic field effect on suppression of Escherichia coli death, S Horiuchi, Y Ishizaki, K Okuno, T Ano, M Shoda, BIOELECTROCHEMISTRY, 53, 2, 149, 153,   2001 03
    Summary:When Escherichia coli B was aerobically grown in a medium containing one-fourth the concentration of the LB medium supplemented with glutamic acid at 43 degreesC under an inhomogeneous 5.2-6.1 T magnetic field, the number of cells in the stationary phase under the high magnetic field was 100,000 times higher than that under a geomagnetic field. The finding that the amount of sigma S factor encoded by the rpoS gene under the high magnetic field was larger than that under the control geomagnetic field indicated that the activity of the rpoS gene was affected by the high magnetic field. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Drastic high magnetic field effect on suppression of Escherichia coli death, S Horiuchi, Y Ishizaki, K Okuno, T Ano, M Shoda, BIOELECTROCHEMISTRY, 53, 2, 149, 153,   2001 03
    Summary:When Escherichia coli B was aerobically grown in a medium containing one-fourth the concentration of the LB medium supplemented with glutamic acid at 43 degreesC under an inhomogeneous 5.2-6.1 T magnetic field, the number of cells in the stationary phase under the high magnetic field was 100,000 times higher than that under a geomagnetic field. The finding that the amount of sigma S factor encoded by the rpoS gene under the high magnetic field was larger than that under the control geomagnetic field indicated that the activity of the rpoS gene was affected by the high magnetic field. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Disappearance of growth advantage in stationary phase (GASP) phenomenon under a high magnetic field, Kazumasa Okuno, Ryo Fujinami, Takashi Ano, Makoto Shoda, Bioelectrochemistry, 53, 2, 165, 169,   2001 , 10.1016/S0302-4598(00)00122-7, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035083197&origin=inward
    Summary:The phenomenon called growth advantage in stationary phase (GASP) originally discovered by Kolter et al. was confirmed using two bacterial strains, Escherichia coli ZK126Nalr and ZK126Smr, under the geomagnetic field. However, when the same experiment was conducted in an inhomogeneous high magnetic field of 5.2-6.1 T, almost no death of ZK126Smr was observed and the GASP phenomenon disappeared. When the same experiment was conducted in a homogeneous magnetic field of 7 T, the GASP was significantly delayed due to the much slower death rate of ZK126Smr than that in the geomagnetic field. These data are consistent with our previous finding that a high magnetic field reduces the death rate of bacteria and enhances their survivability in a stationary phase. Copyright © 2001 Elsevier Science B.V.
  • Drastic high magnetic field effect on suppression of Escherichia coli death, Shin-Ichiro Horiuchi, Yoshimasa Ishizaki, Kazumasa Okuno, Takashi Ano, Makoto Shoda, Bioelectrochemistry, 53, 2, 149, 153,   2001 , 10.1016/S0302-4598(00)00114-8, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035078194&origin=inward
    Summary:When Escherichia coli B was aerobically grown in a medium containing one-fourth the concentration of the LB medium supplemented with glutamic acid at 43°C under an inhomogeneous 5.2-6.1 T magnetic field, the number of cells in the stationary phase under the high magnetic field was 100,000 times higher than that under a geomagnetic field. The finding that the amount of sigma S factor encoded by the rpoS gene under the high magnetic field was larger than that under the control geomagnetic field indicated that the activity of the rpoS gene was affected by the high magnetic field. Copyright © 2001 Elsevier Science B.V.
  • The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production, K Tsuge, T Ano, M Hirai, Y Nakamura, M Shoda, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 43, 9, 2183, 2192,   1999 09
    Summary:Bacillus subtilis YB8 produces the Lipopeptide antibiotic plipastatin, B. subtilis MI113, which is a derivative of strain 168, was converted into a new plipastatin producer, strain 406, by competence transformation with the chromosomal DNA of YB8, Transposon mini-Tn10 insertional mutagenesis was applied to strain 406, which revealed that Ipa-8 (sfp) (encoding 4'-phosphopantetheinyl transferase) and the pps operon (located between 167 and 171 degrees) are essential for plipastatin production. The pps operon was previously suggested to encode putative peptide synthetases (A. Tognoni, E. Franchi, C, Magistrelli, E. Colombo, P. Cosmina, and G. Grandi, Microbiology 141:645-648, 1995) and was thought to be the fengycin operon (V, Tosato, A. M. Albertini, M, Zotti, S, Sonda, and C, V, Bruschi, Microbiology 143:3443-3450, 1997). We claim that the pps operon is the pli operon, encoding plipastatin synthetase. By using a new high-performance liquid chromatography system, we revealed that strain 168 expressing only lpa-8 can also produce plipastatin, although the yield is very low. However, the introduction of the pleiotropic regulator degQ of strain YB8 into strain 168 expressing ipa-g resulted in a 10-fold increase in the production of plipastatin.
  • The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production, K Tsuge, T Ano, M Hirai, Y Nakamura, M Shoda, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 43, 9, 2183, 2192,   1999 09 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032874789&origin=inward
    Summary:Bacillus subtilis YB8 produces the Lipopeptide antibiotic plipastatin, B. subtilis MI113, which is a derivative of strain 168, was converted into a new plipastatin producer, strain 406, by competence transformation with the chromosomal DNA of YB8, Transposon mini-Tn10 insertional mutagenesis was applied to strain 406, which revealed that Ipa-8 (sfp) (encoding 4'-phosphopantetheinyl transferase) and the pps operon (located between 167 and 171 degrees) are essential for plipastatin production. The pps operon was previously suggested to encode putative peptide synthetases (A. Tognoni, E. Franchi, C, Magistrelli, E. Colombo, P. Cosmina, and G. Grandi, Microbiology 141:645-648, 1995) and was thought to be the fengycin operon (V, Tosato, A. M. Albertini, M, Zotti, S, Sonda, and C, V, Bruschi, Microbiology 143:3443-3450, 1997). We claim that the pps operon is the pli operon, encoding plipastatin synthetase. By using a new high-performance liquid chromatography system, we revealed that strain 168 expressing only lpa-8 can also produce plipastatin, although the yield is very low. However, the introduction of the pleiotropic regulator degQ of strain YB8 into strain 168 expressing ipa-g resulted in a 10-fold increase in the production of plipastatin.
  • High magnetic field enhances stationary phase-specific transcription activity of Escherichia coli, K Tsuchiya, K Okuno, T Ano, K Tanaka, H Takahashi, M Shoda, BIOELECTROCHEMISTRY AND BIOENERGETICS, 48, 2, 383, 387,   1999 05 , 10.1016/S0302-4598(99)00023-9, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032953449&origin=inward
    Summary:When Escherichia coli B was aerobically grown at 37 degrees C under inhomogeneous 5.2-6.1 Tesla (T) magnetic fields in the superconducting magnet biosystem (SBS), the cell number in the stationary phase after the growth had leveled off, was about 3 times higher than that under a geomagnetic field. When the E. coli defective in the rpoS gene which encodes a sigma factor, sigma(s) of RNA polymerase and is specifically expressed in the stationary phase was cultivated at 37 degrees C in SBS, such enhancement of cell survival was significantly reduced. The E. coli cells carrying rpoS-lacZ fusion gene or other rpoS dependent genes fused with lacZ were grown, significant increase in the activity of beta-galactosidase was observed in the stationary phase under high magnetic field. These data suggest that enhancement of the transcription activity in stationary phase is involved in the higher survival of the cells under magnetic field. (C) 1999 Elsevier Science S.A. All rights reserved.
  • The genes degQ, pps, and lpa-8(sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production., Kenji TSUGE, Takashi ANO Mitsuyo, HIRAI Yoshiyuki, NAKAMURA Makoto SHODA, Antimicrob. Agents Chemother., 43, 9, 2183, 2192,   1999
  • Control of tomato damping-off caused by Rhizoctonia solani by the heterotrophic nitrifier Alcaligenes faecalis and its product,hydroxylamine., Naohiro HONDA Mitsuyo HIRAI Takashi ANO Makoto SHODA, Ann. Phytopathol. Soc. Jpn., 65, 2, 153, 162,   1999 , 10.3186/jjphytopath.65.153
  • lpa-14, a gene, involved in the production of lipopeptide antibiotics, regulates the production of a sideropbore, 2,3-dihydroxybenzoylglycine, in Bacillus subtilis RB14, CC Huang, ZM Liao, M Hirai, T Ano, M Shoda, JOURNAL OF FERMENTATION AND BIOENGINEERING, 86, 6, 605, 607,   1998 , 10.1016/S0922-338X(99)80015-4, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=13044295990&origin=inward
    Summary:A gene designated lpa-14, which was cloned from Bacillus subtilis RB14 and was associated with the production of lipopeptide antibiotics, was found to be involved in the production of an iron-chelating siderophore, 2,3-dihydroxybenzoylglycine (2,3-DHBG). Although strain R Delta 1, an lpa-14 defective mutant of RB14, showed no growth in iron-deficient medium in contrast to the marked growth of RB14 in the same medium, the addition of chemically synthesized 2,3-DHBG at approximately 100 ppm caused the growth of R Delta 1 to be resumed.
  • Antifungal effect of a heterotrophic nitrifier Alcaligenes faecalis, Naohiro Honda, Mitsuyo Hirai, Takashi Ano, Makoto Shoda, Biotechnology Letters, 20, 7, 703, 705,   1998 , 10.1023/A:1005382810088, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031840796&origin=inward
    Summary:Alcaligenes faecalis suppressed the growth of 11 strains of fungal plant pathogens in vitro. When it was cultivated on a synthetic medium containing (NH4)2SO4 as the sole nitrogen source, NH2OH, NO2- and NO3- were produced, indicating that heterotrophic nitrification was occurring. The suppressive effect of A. faecalis on plant pathogens was due to its NH2OH produced.
  • Effect of high magnetic field on the growth of Bacillus subtilis measured in a newly developed superconducting magnet biosystem, K Nakamura, K Okuno, T Ano, M Shoda, BIOELECTROCHEMISTRY AND BIOENERGETICS, 43, 1, 123, 128,   1997 06 , 10.1016/S0302-4598(96)05163-X, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031171535&origin=inward
    Summary:A new superconducting magnet biosystem (SBS) has been developed, which can provide a magnetic field of 0.5-7 T, where biological reactions can be conducted under temperature-controlled conditions. The aerobic growth of a bacterium, Bacillus subtilis MI113, was investigated under homogeneous (7 T) and inhomogeneous (5.2-6.1 T) magnetic fields in a shaken culture. In the stationary phase, the cell number in an inhomogeneous magnetic field was about twofold higher than that of the reference, indicating that the magnitude of the decrease in the cell number was reduced by the high magnetic field. The slower decline in the cell number in a magnetic field was due to the slower death rate of the vegetative cells. The inhibition of spore formation from vegetative cells was also observed in a magnetic field, which was reflected by the reduced activity of aikaline phosphatase. Genetically transformed B. subtilis MI113 (pC112) produced a higher concentration of a lipopeptide antibiotic, surfactin, in the stationary phase in an inhomogeneous magnetic field due to the higher cell number of the transformant in the magnetic field. (C) 1997 Elsevier Science S.A.
  • Use of soybean curd residue, okara, for the solid state substrate in the production of a lipopeptide antibiotic, iturin A, by Bacillus subtilis NB22, A Ohno, T Ano, M Shoda, PROCESS BIOCHEMISTRY, 31, 8, 801, 806,   1996 11 , 10.1016/S0032-9592(96)00034-9, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0030295093&origin=inward
    Summary:The possibility of the utilization of soybean curd residue, okara, for the production of a lipopeptide antibiotic, iturin A, in solid state fermentation (SSF) by Bacillus subtilis NB22 was investigated. Okara is a by-product of the tofu manufacturing process, now treated as an industrial waste and disposed of mostly by incineration. Dehydrated okara, with improved transportability and preservability, could be used as effectively as the fresh, intact okara for SSF by B. subtilis NB22 for the production of iturin A. Copyright (C) 1996 Elsevier Science Ltd.
  • Persistence of Bacillus subtilis RB14 and its derivative strains in soil with respect to the lpa-14 gene, O Asaka, T Ano, M Shoda, JOURNAL OF FERMENTATION AND BIOENGINEERING, 81, 1, 1, 6,   1996 , 10.1016/0922-338X(96)83110-2, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0030050664&origin=inward
    Summary:Bacillus subtilis RB14 produces the antibiotics iturin A and surfactin, and expresses broad suppressibility against several phytopathogenic microorganisms. The gene lpa-14, cloned from RB14, is responsible for the production of both antibiotics. In this study, the contribution of lpa-14 to the persistence of strain RB14 or its derivative strains in soil was investigated. Strain RB14-C is a spontaneous streptomycin-resistant mutant of RB14. When its survival in soil was examined at 30 degrees C, immediately after the inoculation of vegetative cells, rapid sporulation occurred until the cell population was stabilized as spores at a level of over 10(7) cfu/g-dry soil. At the same temperature, both R Delta 1, a mutant in which lpa-14 was deleted, and R Delta 1(pC115), a transformant in which antibiotic productivity was restored by transforming Rbl with a plasmid carrying lpa-14, showed similar soil population dynamics to those of RB14-C. At 15 degrees C, although the rate of decline in the total cell number and the sporulation rate of RB14-C were significantly reduced, the numbers of cells in the soil was finally stabilized at the same level as at 30 degrees C. However, R Delta 1 cells were stabilized at a much lower level at 15 degrees C, mainly due to a higher death rate of vegetative cells and a slower sporulation rate. This was especially significant in non-sterile soil, presumably due to a reduced ability to compete with indigenous soil microflora caused by the loss of iturin A and surfactin production. In the case of R Delta 1(pC115), the survival rate of vegetative cells and sporulation ability at 15 degrees C were not completely restored to the levels of those of RB14-C. The results showed that lpa-14 was associated not only with the production of lipopeptides but also with survival of vegetative cells and the decline of the sporulation rate at 15 degrees C.
  • Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surfactin in Bacillus subtilis YB8, Kenji Tsuge, Takashi Ano, Makoto Shoda, Archives of Microbiology, 165, 4, 243, 251,   1996 , 10.1007/s002030050322, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029886333&origin=inward
    Summary:Bacillus subtilis YB8 was found to produce the lipopeptide antibiotics surfactin and plipastatin B1. A gene, lpa-8, required for the production of both lipopeptides was cloned from strain YB8. When this gene was inactivated in strain YB8, neither surfactin nor plipastatin B1 was produced. However, the defective strain transformed with an intact lpa-8 gene had restored ability to produce both peptides. Nucleotide sequence analysis of the region essential for the production of the peptides revealed the presence of a large open reading frame. The deduced amino acid sequence of lpa-8 (224 amino acid residues) showed sequence similarity to that of sfp (from surfactin-producing B. subtilis), lpa-14 (from iturin A- and surfactin-producing B. subtilis), psf-1 (from surfactin-producing Bacillus pumilus), gsp (from gramicidin-S-producing Bacillus brevis), and entD (from siderophore-enterobactin-producing Escherichia coli), which are able to complement a defect in the sfp, gene and promote production of the lipopeptide antibiotic surfactin. The sequence similarity among these proteins and the product similarity of cyclic peptides suggests that they might be involved in the biosynthesis or secretion of the peptides.
  • Effect of homogeneous and inhomogeneous high magnetic fields on the growth of Escherichia coli, K Tsuchiya, K Nakamura, K Okuno, T Ano, M Shoda, JOURNAL OF FERMENTATION AND BIOENGINEERING, 81, 4, 343, 346,   1996 , 10.1016/0922-338X(96)80588-5, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029933711&origin=inward
    Summary:The growth of Escherichia coli B was investigated under homogeneous 7 Tesla (T) magnetic field, and inhomogeneous 5.2 similar to 6.1 T and 3.2 similar to 6.7 T magnetic fields which are produced by a newly constructed superconducting magnet biosystem (SBS). These high magnetic fields adversely affected on the growth of the bacterium in the early logarithmic growth phase. However, in the stationary phase, the cell number under a high magnetic field was about 2 similar to 3 times higher than that of a control, indicating that the magnitude of the decrease in the cell number was reduced by the high magnetic field. The effect of the inhomogeneous magnetic field was much stronger than that of the homogeneous one. The high magnetic field was found to affect the cells of the bacterium differently, depending on the growth phases.
  • Characterization of Bacillus subtilis YB8, coproducer of lipopeptides surfactin and plipastatin B1, K Tsuge, T Ano, M Shoda, JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 41, 6, 541, 545,   1995 12 , 10.2323/jgam.41.541, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029585402&origin=inward
  • PRODUCTION OF A LIPOPEPTIDE ANTIBIOTIC, SURFACTIN, BY RECOMBINANT BACILLUS-SUBTILIS IN SOLID-STATE FERMENTATION, A OHNO, T ANO, M SHODA, BIOTECHNOLOGY AND BIOENGINEERING, 47, 2, 209, 214,   1995 07 , 10.1002/bit.260470212, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029329111&origin=inward
    Summary:Production of a lipopeptide antibiotic, surfactin, in solid state fermentation (SSF) on soybean curd residue, Okara, as a solid substrate was carried out using Bacillus subtilis MI113 with a recombinant plasmid pC112, which contains Ipa-14, a gene related to surfactin production cloned at our laboratory from a wild-type surfactin producer, B. subtilis RB14. The optimal moisture content and temperature for the production of surfactin were 82% and 37 degrees C, respectively. The amount of surfactin produced by MI113 (pC112) was as high as 2.0 g/kg wet weight, which was eight times as high as that of the original B. subtilis RB14 at the optimal temperature for surfactin production, 37 degrees C. Although the stability of the plasmid showed a similar pattern in both SSF and submerged fermentation (SMF), production of surfactin in SSF was 4-5 times more efficient than in SMF. (C) 1995 John Wiley and Sons, Inc.
  • SURVIVAL OF BACILLUS-SUBTILIS NB22 AND ITS TRANSFORMANT IN SOIL, Y TOKUDA, T ANO, M SHODA, APPLIED SOIL ECOLOGY, 2, 2, 85, 94,   1995 06
    Summary:Bacillus subtilis NB22 is an antifungal-antibiotic (iturin) producer that expresses broad suppressibility of phytopathogenic fungi and bacteria and is considered as a potential biological pesticide. The survival of B. subtilis NB22-1, which is a spontaneous streptomycin-resistant mutant of NB22 and its transformants with plasmid DNA, was investigated in soil. The number of vegetative cells of the host strain NB22-1 declined initially in soil at 15 degrees C, but the population was stabilized at the level of spores which were formed during incubation in soil. Neither nutritional amendment nor the raising of temperature were effective for the higher induction of spore formation in soil. However, simultaneous treatment of increasing the temperature and nutritional amendment was effective for the rapid induction of spores in the soil, which led to stabilization of the population at the level of 10(7) spores g(-1) dry soil of the initially added cell number. Vegetative cells of the transformants NB22-1(pC194) and NB22-1(pUB110) also sporulated efficiently in soil under these conditions and were stable for 50 days, but died out within 10 days without such treatment. The stability of plasmid pUB110 was high (around 90%), but pC 194 was quickly cured and its stability declined to about 50%. The stability of the two plasmids was similar to that in liquid culture using sporulation medium. When spores of NB22-1 or its transformants were introduced into the soil, they persisted at high level and showed no loss in their viability until the end of the experiment at 50 days.
  • EFFECT OF TEMPERATURE ON PRODUCTION OF LIPOPEPTIDE ANTIBIOTICS, ITURIN A AND SURFACTIN BY A DUAL PRODUCER, BACILLUS-SUBTILIS RB14, IN SOLID-STATE FERMENTATION, A OHNO, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 80, 5, 517, 519,   1995 , 10.1016/0922-338X(96)80930-5, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029589696&origin=inward
    Summary:Temperature dependency of the production of iturin A and surfactin by a dual producer, Bacillus subtilis RB14, in the solid-state fermentation of okara was investigated. The optimal temperature for burin A was 25 degrees C, while that for surfactin was 37 degrees C, in spite of their dependency on a common gene, lpa-14. When the effect of temperature on the relative ratios of the amount of the five homologues of iturin A to the total iturin A produced by RB14 was investigated, only the ratios of homologues having beta-amino acid residues with normal aliphatic chains were affected and the ratio of the homologue with longer normal (C-16-) chain increased as the temperature was increased.
  • NEW 7-T SUPERCONDUCTING MAGNET SYSTEM FOR BACTERIAL CULTIVATION, M OKUDA, K SAITO, T KAMIKADO, S ITO, K MATSUMOTO, K OKUNO, K TSUCHIYA, T ANO, M SHODA, CRYOGENICS, 35, 1, 41, 47,   1995 01 , 10.1016/0011-2275(95)90424-E, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0002536881&origin=inward
    Summary:A new superconducting magnet system for bacterial cultivation has been developed. The superconducting magnet has a horizontal room temperature bore with a diameter of 160 mm, and provides a homogeneous magnetic field of 7 T +/- 0.5% for a 200 mm long x 100 mm diameter region. This homogeneous field region contains an incubator, where bacteria are cultivated aerobically at 10-70-degrees-C +/- 0.1-degrees-C while being shaken. The culture exposed to the high magnetic field is compared with a control culture incubated at below geomagnetic field strength. Cultivation of Escherichia coli was carried out both in homogeneous and in inhomogeneous fields, and 1.4-3.6 times the number of viable cells of the control culture was observed in a stationary phase.
  • A RAPID AND SIMPLE METHOD FOR PLASMID COPY NUMBER COMPARISON, O ASAKA, T ANO, M SHODA, BIOTECHNOLOGY TECHNIQUES, 8, 12, 865, 868,   1994 12 , 10.1007/BF02447729, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0028251499&origin=inward
    Summary:A rapid, simple, and sensitive method for plasmid copy number comparison was developed. The extracted plasmids from the same; amount of cells were subjected to agarose gel electrophoresis and the gels photographed. The photographs were processed by a Macintosh image analyser;to enumerate the densities of plasmid bands. As a size reference, lambda -DNA digested with a restriction enzyme was used. The densities divided by size of plasmids (base pair) would represent relative values of their copy numbers.
  • A NEW CULTIVATION SYSTEM OPERATED UNDER A SUPER HIGH MAGNETIC-FIELD, K OKUNO, K TSUCHIYA, T ANO, M SHODA, M OKUDA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 77, 4, 453, 456,   1994 , 10.1016/0922-338X(94)90026-4, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0028332544&origin=inward
    Summary:A new cultivation system which operates under a super high magnetic field in the range of 0.5 to 7 Tesla was developed. In this reactor system, the effects of a high magnetic field on various biological reactions could be investigated not only under a homogeneous high magnetic field, but also under a gradient magnetic held. The maximum magnetic field gradient is 23 Tesla/m. Since the superconductive magnet was designed so as to significantly reduce the evaporation rates of liquid helium and nitrogen, operation is virtually maintenance-free compared with that in conventional superconductive magnets. The temperature of the reactor is controllable within the range of 10 to 70 degrees C (deviations of +/- 0.1 degrees C). When Escherichia coil B cells were grown at 37 degrees C, total cell number during the stationary phase was 70% higher under homogeneous 7 Tesla conditions than in the geomagnetic field. A three fold larger cell number was detected in a gradient magnetic fields between 5 and 6 Tesla.
  • ISOLATION OF ELECTRIC PULSE-INDUCED ESCHERICHIA-COLI GENES BY OPERON FUSION TECHNIQUES, K TSUCHIYA, W MIYAGI, T ANO, M SHODA, BIOTECHNOLOGY TECHNIQUES, 7, 10, 719, 722,   1993 10 , 10.1007/BF00152619, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34250076470&origin=inward
    Summary:A search of electric-pulse induced genes in Escherichia coli was carried out by operon fusion techniques with a hybrid bacteriophage Mu, which creates transcriptional fusions of the structural gene of beta-galactosidase to the host. Among two hundred transductants tested, nine colonies showed higher expression of more than twofold when they were treated with electric pulses. An electric-pulse stimulated transductant was not stimulated by UV irradiation, which is known to induce an SOS response. Conversely, strain PQ37, which has an operon fusion in one of the SOS genes, did not respond to an electric pulse treatment. A possibility of the presence of the electric-pulse stimulated genes, which were not induced by DNA damages, was suggested.
  • EFFECT OF TEMPERATURE-CHANGE AND AERATION ON THE PRODUCTION OF THE ANTIFUNGAL PEPTIDE ANTIBIOTIC ITURIN BY BACILLUS-SUBTILIS NB22 IN LIQUID CULTIVATION, A OHNO, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75, 6, 463, 465,   1993 , 10.1016/0922-338X(93)90098-S, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027248853&origin=inward
    Summary:The effects of temperature and air supply on the production of the antifungal lipopeptide antibiotic, iturin by Bacillus subtilis NB22 in a 5-d course of liquid cultivation were investigated on a flask scale. Changing the temperature from 30-degrees-C to 25, 37, 42, or 48-degrees-C for one day during the 5-d cultivation reduced the amount of iturin producted in comparison with cultivation at a constant 30-degrees-C. The temperature on the first day was notably critical. When the air supply was varied by changing the pings of the shaking flasks, the air supply was also critical on the first day. When cells spent the first day with an inappropriate temperature or air supply, the decrease in iturin production could not be compensated for during the subsequent 4-d cultivation at an adequate temperature and air supply. Although iturin production was significant on the second and third days when the growth of the bacterium had leveled off, the initial conditions severely affected the yield of the product.
  • Plasmid Instability in Bacillus subtilis during Sporulation, Orie Asaka, Yukinori Tokuda, Takashi Ano, Makoto Shoda, Bioscience, Biotechnology and Biochemistry, 57, 2, 336, 337,   1993 , 10.1271/bbb.57.336, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=9044220920&origin=inward
  • PRODUCTION OF THE ANTIFUNGAL PEPTIDE ANTIBIOTIC, ITURIN BY BACILLUS-SUBTILIS NB22 IN SOLID-STATE FERMENTATION, A OHNO, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75, 1, 23, 27,   1993 , 10.1016/0922-338X(93)90172-5, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027474288&origin=inward
    Summary:Production of iturin, an antifungal peptide effective at suppressing phytopathogens, by Bacillus subtilis NB22 was investigated in solid state fermentation (SSF) using soy bean curd residue (okara). In scale-up from 15 g to 3 kg, aeration, temperature, and moisture content were controlling factors for the efficient production of iturin. It was found that solid state fermentation was 6-8 times more efficient with respect to iturin productivity than submerged fermentation on the basis of unit wet weight. Higher productivity in selective production of specific components of iturin which are stronger inhibitors of plant pathogens was also confirmed in SSF.
  • CHARACTERISTICS OF PLASMID STABILITY IN BACILLUS-SUBTILIS NB22, AN ANTIFUNGAL-ANTIBIOTIC ITURIN PRODUCER, Y TOKUDA, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75, 4, 319, 321,   1993 , 10.1016/0922-338X(93)90159-6, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027308794&origin=inward
    Summary:The stability of plasmids pC194 and pUB110 was investigated in an antifungal antibiotic iturin producer, Bacillus subtilis NB22. Although plasmid pC194 was maintained stably over a hundred generations in five succesive cultivations in iturin-production no. 3 medium, significant curing was observed when the cultivation was prolonged for 10 d in the same medium. When the transformant of pC194 was cultivated in Schaeffer's sporulation medium, drastic curing took place in accordance with the occurrence of sporulation, even in the presence of an antibiotic for selective pressure. In the case of pUB110, in sharp contrast to the result with pC194, high stability was observed in the sporulation medium, but significant curing was observed during prolonged incubation in no. 3 medium.
  • EFFECT OF SUPER HIGH MAGNETIC-FIELD ON THE GROWTH OF ESCHERICHIA-COLI UNDER VARIOUS MEDIUM COMPOSITIONS AND TEMPERATURES, K OKUNO, K TUCHIYA, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75, 2, 103, 106,   1993 , 10.1016/0922-338X(93)90218-W, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027419720&origin=inward
    Summary:When two strains of Escherichia coli were grown under a super high magnetic field (11.7 Tesla) in complex medium, the growth was stimulated in comparison with that in the geomagnetic field. When the bacteria were grown in synthetic medium, the growth rates were reduced significantly. As a result of the addition of casamino acids to the synthetic medium, the growth was shifted from a reduced state to a stimulated one, suggesting that certain amino acids are responsible for the phenomenon. Twenty amino acids were thus added individually to minimal medium; some of these amino acids shifted the reduced growth state to accelerated or normal growth. The critical concentration of glutamic acid for the growth shift was determined to be 0.010. 1 mg/l. When the bacteria were grown at temperatures lower than the optimal temperature for growth, the 11.7 T magnetic field enhanced the growth rate irrespective of media used, while at higher temperatures reduced growth became significant in accordance with the increase in temperature. A potential use of the high magnetic field as a control factor in biological system is suggested.
  • SURVIVAL OF BACILLUS-SUBTILIS NB22, AN ANTIFUNGAL-ANTIBIOTIC ITURIN PRODUCER, AND ITS TRANSFORMANT IN SOIL-SYSTEMS, Y TOKUDA, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75, 2, 107, 111,   1993 , 10.1016/0922-338X(93)90219-X, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027418230&origin=inward
    Summary:Bacillus subtilis NB22 is an antifungal-antibiotic iturin producer that expresses broad suppressibility against phytopathogenic microorganisms. The survival of B. subtilis NB22-1, which is a spontaneous streptomycin-resistance mutant of NB22 was investigated in four different soils. After a gradual decline, the bacterial viable cell number stabilized at a level of 10(4)-10(5) colony forming units/g-dry soil irrespective of soil differences. A similar decline and stabilization pattern was observed in the case of the transformant of B. subtilis NB22-1 with the plasmid pC194 in nonsterile soil. The transformant reached a much higher stabilized level in sterile soil than in nonsterile soil. However, significant loss of the plasmid was observed in both the soil systems after 10 to 20 d incubation. Plasmid pC194 was stable over a hundred generations in the strain when cultivated in a liquid complex medium, but unstable in minimal medium, indicating that the plasmid stability in soil does not necessarily reflect that in liquid culture.
  • NUCLEOTIDE-SEQUENCE AND CHARACTERISTICS OF THE GENE, LPA-14, RESPONSIBLE FOR BIOSYNTHESIS OF THE LIPOPEPTIDE ANTIBIOTICS ITURIN-A AND SURFACTIN FROM BACILLUS-SUBTILIS RB14, CC HUANG, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 76, 6, 445, 450,   1993 , 10.1016/0922-338X(93)90238-4, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027771578&origin=inward
    Summary:Bacillus subtilis RB14 is a coproducer of the lipopeptide antibiotics iturin A and surfactin. Iturin A and surfactin have a similar structure consisting of a seven-amino-acid cyclic peptide, linked by either hydroxy or ester peptide linkage, respectively, to the fatty acid part. A 10-kb fragment responsible for the production of the lipopeptide antibiotics iturin A and surfactin in B. subtilis RB14 was minimized and the nucleotide sequence of the region essential for the synthesis of the lipopeptides was determined. A large open reading frame consisting of 224 amino acid residues was found. The gene, designated as lpa-14 (lipopeptide antibiotic production of RB14), showed high homology with sfp and psf-1 (regulators for surfactin production in B. subtilis and Bacillus pumilus, respectively) and an unknown open reading frame, orfX, in the upstream region of the peptide antibiotic gramicidin S biosynthesis operon of Bacillus brevis. The biosynthesis of surfactin and of iturin A was shown to be coregulated by the same gene, lpa-14. It is suggested that a common regulation system might exist among these genes for the production of peptide antibiotics in Bacillus species.
  • PRODUCTION OF A LIPOPEPTIDE ANTIBIOTIC SURFACTIN WITH RECOMBINANT BACILLUS-SUBTILIS, A OHNO, T ANO, M SHODA, BIOTECHNOLOGY LETTERS, 14, 12, 1165, 1168,   1992 12 , 10.1007/BF01027022, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027052446&origin=inward
    Summary:Production of a lipopeptide antibiotic surfactin was carried out using a recombinant Bacillus subtilis. Surfactin yield of the recombinant strain was about one and half times as much as that of Bacillus subtilis RB14, the strain in which the surfactin gene was originated. This system is especially noteworthy because a recombinant strain surpassed the original strain in the production of a bacterial antibiotic as a secondary metabolite of the bacterium.
  • CHARACTERIZATION OF BACILLUS-SUBTILIS RB14, COPRODUCER OF PEPTIDE ANTIBIOTICS ITURIN-A AND SURFACTIN, H HIRAOKA, O ASAKA, T ANO, M SHODA, JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 38, 6, 635, 640,   1992 12 , 10.2323/jgam.38.635, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026984355&origin=inward
  • EFFECT OF SUPER HIGH MAGNETIC-FIELD ON LYSOGENIC LAMBDA-PHAGE WITH A TEMPERATURE SENSITIVE REPRESSOR, K OKUNO, T ANO, M SHODA, BIOTECHNOLOGY LETTERS, 14, 9, 765, 768,   1992 09 , 10.1007/BF01029135, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026745049&origin=inward
    Summary:Escherichia coli where bacteriophage lambda was lysogenic was grown under the super high magnetic field (11.7 Tesla) and the effect of the field on the transition from lysogenic to lytic process of the phage was investigated. The occurrence of phage particles due to induction of phage was stimulated under 11.7 T in comparison with that in geomagnetic field by raising temperature from 30 to 45-degrees-C. Especially at 35-degrees-C, the phage titer was tenfold larger. No significant effect of the field on the phage particle per se was observed. A potential application of high magnetic strength as a controlling factor of in vivo switching was implied.
  • PRODUCTION OF ANTIFUNGAL ANTIBIOTIC, ITURIN IN A SOLID-STATE FERMENTATION BY BACILLUS-SUBTILIS NB22 USING WHEAT BRAN AS A SUBSTRATE, A OHNO, T ANO, M SHODA, BIOTECHNOLOGY LETTERS, 14, 9, 817, 822,   1992 09 , 10.1007/BF01029145, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026782409&origin=inward
    Summary:Production of a family of lipopeptide antibiotic, iturin by B. subtilis NB22, in solid state fermentation (SSF) of wheat bran (WB) was investigated. The amount of iturin produced per unit weight of wet substrate was 5-6 times more than that in the submerged fermentation (SMF). SSF enabled to produce a homologue of iturin with strong antibiotic activity in a larger fraction compared with the SMF.
  • HIGH-EFFICIENCY TRANSFORMATION OF BACILLUS-SUBTILIS NB22, AN ANTIFUNGAL ANTIBIOTIC ITURIN PRODUCER, BY ELECTROPORATION, Y MATSUNO, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 73, 4, 261, 264,   1992 , 10.1016/0922-338X(92)90179-X, http://ci.nii.ac.jp/naid/110002681360
    Summary:High-voltage electroporation was used to transform Bacillus subtilis NB22, an antifungal antibiotic producer, reaching the efficiency of 10(7) transformants/mu-g plasmid DNA. Transformation frequency was dependent on the composition of the electroporation solution, the electrical field strength and the cell concentration. Addition of polyethylene glycol (PEG) and mannitol in the transformation solution was critical for a high efficiency of transformation.
  • RAPID TRANSFORMATION OF BACILLUS-SUBTILIS USING KCL-TREATMENT, H HIRAOKA, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 74, 4, 241, 243,   1992 , 10.1016/0922-338X(92)90119-F, http://ci.nii.ac.jp/naid/110002681468
    Summary:An optimal simple and quick method for transforming Bacillus subtilis by plasmid DNA was developed. Cells exposed to 410 mM KCl solution were transformed with plasmid DNA in the presence of 35% polyethylene glycol. All steps before the gene expression stage were completed within an hour, and several thousand transformants were obtained from one mug of plasmid DNA. The activation of autolysin(s) of the cells is believed to be involved in the mechanisms of this transformation.
  • MOLECULAR-CLONING OF A GENE RESPONSIBLE FOR THE BIOSYNTHESIS OF THE LIPOPEPTIDE ANTIBIOTICS ITURIN AND SURFACTIN, H HIRAOKA, T ANO, M SHODA, JOURNAL OF FERMENTATION AND BIOENGINEERING, 74, 5, 323, 326,   1992 , 10.1016/0922-338X(92)90068-6, http://ci.nii.ac.jp/naid/110002681485
    Summary:A gene responsible for the production of iturin and surfactin was cloned and analyzed in the original wild strain Bacillus subtilis RB14, a coproducer of surfactin and iturin, as well as in B. subtilis MI113, a derivative of strain B. subtilis 168. The cloned gene lpa (lipopeptide antibiotic production) permitted only surfactin production in strain MI113, but when it was destroyed in RB14, the defective strain simultaneously lost the ability to produce both surfactin and iturin. The ability was restored by the introduction of the intact lpa gene into the strain.
  • Transformation of Bacillus subtilis, antifungal-antibiotic iturin producers with isolated antibiotic resistance plasmids, Y. Matsuno, T. Hitomi, T. Ano, M. Shoda, Journal of General and Applied Microbiology, 38, 1, 13, 21,   1992 , 10.2323/jgam.38.13, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026668310&origin=inward
    Summary:In order to obtain appropriate vectors to transform several strains of <i>Bacillus subtilis</i>, antifungal iturin producers, plasmids have been isolated as covalently closed circular deoxyribonucleic acid from antibiotic-resistant bacteria inhabited in composts. Eight plasmids coding antibiotic resistance transformed both competent cells of a derivative of <i>B. subtilis</i> Marburg 168 and protoplasts of <i>B. subtilis</i> NB22, an antifungal-antibiotic iturin producer, in the presence of polyethylene glycol. However, transformation efficiency was not high enough as a cloning host-vector system. To improve transformation efficiency, KCl-treatment method and electroporation method were applied to four iturin producers, and electroporation method was most effective for transformation with newly-isolated plasmids with great efficiency.
  • EFFECT OF SUPER HIGH MAGNETIC-FIELD ON THE GROWTH OF ESCHERICHIA-COLI, K OKUNO, T ANO, M SHODA, BIOTECHNOLOGY LETTERS, 13, 10, 745, 750,   1991 10 , 10.1007/BF01088180, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0026047214&origin=inward
    Summary:Auxotrophic mutants of Escherichia coli were grown under the super high magnetic field (11.7 Tesla) and the effect of the field both on the growth and mutation frequency of the bacteria was investigated. When the bacteria were cultivated in complex media, the growth was stimulated under 11.7 T in comparison with that in geomagnetic field. When the bacteria were grown in synthetic media, the growth rates were reduced significantly. Neither mutagenic nor lethal effects of the magnetic field on the bacteria was observed. A potential application of high magnetic strength as a controlling factor of the bacterial growth was implied.
  • Suppressive effect in vitro of magnesium L-ascorbyl-2-phosphate (APM), an ascorbic acid derivative, on the impairment of DNA by glucose-6-phosphate, TAKASHI ANO, Agricultural and Biological Chemistry, 55, 12,   1991
  • Effect of super high magnetic field on the growth of Escherichia coli, TAKASHI ANO, Biotechnology Letters, 13, 10,   1991
  • Suppressive effect in vitro of magnesium L-ascorbyl-2-phosphate (APM), an ascorbic acid derivative, on the impairment of DNA by glucose-6-phosphate, Takashi Ano, Akihiro Ohno, Makoto Shoda, Agricultural and Biological Chemistry, 55, 12, 2967, 2970,   1991 , 10.1080/00021369.1991.10859831, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84954868807&origin=inward
    Summary:We attempted to find compounds that suppress the DNA impairment caused by D-glucose-6-phosphate (Glc-6-P) by observing the loss of ability of pBR322 to transform Escherichia coli, and found that APM (magnesium L-ascorbyl-2-phosphate) suppressed the loss of transformability to less than 1/100 of that in the absence of APM. Wiien 2′-deoxyguanosine 5′-monophosphate (dGMP) was incubated in the dark with Glc-6-P and APM, changes in their absorbance patterns were observed, indicating possible suppression by APM of the interactions between dGMP and Glc-6-P. APM weakly suppressed the Maillard reaction. © 1991 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry.
  • TRANSFORMATION OF BACILLUS-SUBTILIS WITH THE TREATMENT BY ALKALI CATIONS, T ANO, A KOBAYASHI, M SHODA, BIOTECHNOLOGY LETTERS, 12, 2, 99, 104,   1990 02 , 10.1007/BF01022423, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025217712&origin=inward
    Summary:Exposing Bacillussubtilis cultures to high concentrations of alkali cations, especially K+, allows efficient transformation by plasmids. The method allows transformation with unfractionated plasmid DNA, monomeric plasmid DNA as well as linear plasmid DNA. B.subtilis strains, not amenable to natural transformation, were also transformed by the present method. © 1990 Kluwer Academic Publishers.
  • Plasmid transformation in Bacillus subtilis NB22, an antifungal-antibiotic iturin producer, TAKASHI ANO, FEMS Microbiology Letters, 67, 1,   1990
  • COMPLETE NUCLEOTIDE-SEQUENCES OF BACILLUS PLASMIDS PUB110DB, PRBH1 AND ITS COPY MUTANTS, RE MULLER, T ANO, T IMANAKA, S AIBA, MOLECULAR & GENERAL GENETICS, 202, 1, 169, 171,   1986 , 10.1007/BF00330534, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0022588798&origin=inward
    Summary:The deletion plasmids, pRBH1 (1.5 MDa, kanamycin resistance, Kmr) and pUB110dB (1.5 MDa, Kmr), were obtained from pTB913 (2.9 MDa, Kmr, isolated from a thermophilic bacillus) and pUB110 (3.0 MDa, Kmr, from Staphylococcus aureus), respectively. All the nucleotide sequences of these deletion plasmids were determined. Replication origin regions of pRBH1 and pUB110dB contained, respectively, 63 base-pair inverted repeat and a large open reading frame, encoding RepB protein (235 amino acid residues). The nucleotide sequences were identical to each other except for one base in the center of the inverted repeat. Two copy number mutant plasmids, pRBHC3 and pRBHC7, were obtained from pRBH1. The mutation points were located at different positions in the RepB protein coding region (Gly to Asp for pRBHC3 and Gly to Glu for pRBHC7). RepB protein was shown to be involved in the copy number control of these plasmids. © 1986 Springer-Verlag.
  • THE COPY NUMBER OF BACILLUS PLASMID PRBH1 IS NEGATIVELY CONTROLLED BY REPB PROTEIN, T ANO, T IMANAKA, S AIBA, MOLECULAR & GENERAL GENETICS, 202, 3, 416, 420,   1986 , 10.1007/BF00333271, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0022569966&origin=inward
    Summary:The replication control mechanism of Bacillus plasmid pRBH1 was analysed; pRBH1 contains four promoters, P1 to P4, and a large inverted repeat (63 base pairs) upstream of the protein (RepB) coding sequence. The stem and loop structure is surrounded by two promoters, P1 and P3, with different directions of transcription. One base substitution in the loop structure caused a change in copy number. Since the P1 promoter is located upstream of the replication origin of pRBH1, the transcript from the P1 promoter might serve as the primer of DNA replication. In vivo transcription from the P1 promoter was repressed by a trans-acting plasmid gene product. Since the RepB protein is involved in copy number control and RepB contains the consensus amino acid sequence of DNA binding proteins, RepB was thought to be the repressor. It was concluded from these data that the inverted repeat is involved in the control of copy number of the plasmid pRBH1. The RepB protein also contains two regions highly homologous with the Rom protein encoded on Escherichia coli plasmid ColE1. The possible mechanism for the copy number control of the plasmid via RepB protein and/or RNAs is discussed. © 1986 Springer-Verlag.
  • DENOVO SYNTHESIS OF POLY(DAT) BY THE CELL EXTRACT OF A THERMOPHILE BACILLUS-STEAROTHERMOPHILUS CARRYING A PLASMID PTB913, T ANO, T IMANAKA, S AIBA, FEMS MICROBIOLOGY LETTERS, 26, 2, 131, 134,   1985 , 10.1111/j.1574-6968.1985.tb01579.x, http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021942093&origin=inward
    Summary:In the absence of added template and primer, DNA synthesis activity which required dATP, dTTP, magnesium ion, and ATP was detected in the cell extracts prepared from a thermophile Bacillus stearothermophilus carrying a plasmid pTB913, but not from the strain without plasmid. Polymer synthesis was detectable only after a lag period and then proceeded at an exponential rate. The DNA synthesized in vitro was the alternating copolymer of dAMP and dTMP, poly(dAT). This reaction was very similar to the de novo DNA synthesis by DNA polymerase I of Escherichia coli, Bacillus subtilis, and Micrococcus luteus, except for the requirement of ATP and thermostability. © 1985.

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Effective utilization of C5 sugars through the use of biofilm reactor, The main components of plant biomass are carbohydrates, a mixture of cellulose, and hemicellulose. When cellulose and hemi-cellulose are hydrolyzed, their constituent sugars, glucose(a six carbon sugar, hence "C6") and xylose(a five carbon sugar, hence,"C5"), are produced respectively. As a method of metabolizing the C5 sugars is required to utilize the biomass effectively, Bacillus subtilis was used to metabolize the C5 sugar. Production of a useful antibiotic as a model compound was employed, and the higher productivity was observed, and the usefulness of C5 sugars were shown.
  • Study on the Effect of Super-High Magnetic Field on the Biological Systems
  • Study on the Development of a new Biological Control Agent