KINDAI UNIVERSITY


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SUZUKI Takahiro

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FacultyDepartment of Biotechnological Science / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/146-suzuki-takahiro.html
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Last Updated :2020/09/30

Education and Career

Education

  •   1979 04  - 1983 03 , Nagoya Univ., Faculty of Agriculture
  •   1983 04  - 1988 03 , Nagoya University graduate school, Doctor course of Agriculture

Academic & Professional Experience

  •   2010 04 ,  - 現在, Professor, Faculty of Biology-Oriented Science & Technology, Kinki University
  •   2004 11 ,  - 2010 03 , manager, Foundation Laboratory, Nihon L'Oreal
  •   2000 12 ,  - 2004 10 , Chief Researcher of Cosmetics, mica division, Topy Industries
  •   2000 08 ,  - 2000 11 , Hitech ht Co., Ltd.
  •   1996 04 ,  - 2000 03 , Faculty of Industrial Science and Technology, Biological Science and Technology, Tokyo University of Science
  •   1992 09 ,  - 1993 09 , Guest researcher, Histochemisty dept., Royal Postgraduate Medical School
  •   1988 04 ,  - 1989 03 , Massachusetts Institute of Technology

Research Activities

Research Areas

  • Life sciences, Applied microbiology, methane fermentation
  • Environmental science/Agricultural science, Crop production science, biomass production
  • Nanotechnology/Materials, Energy chemistry, photosynthesis energy
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering, bioreactor system
  • Nanotechnology/Materials, Composite materials and interfaces, cosmetic powder technology
  • Nanotechnology/Materials, Nanomaterials, cosmetics
  • Environmental science/Agricultural science, Environmental materials/recycling technology, renewable energy
  • Environmental science/Agricultural science, Recycling systems and society, renewable energy

Research Interests

  • potato energy, perfusion fermentaion system, surface treatment, phototrophic bacteria, lactic acid fermentation, cyanobacteria, biodegradable polymer, ceramic memblane filter, hydroxy apatite, bioceramics, poly-β-hydroxybutylic acid, osteoblast cell, fibroblast cells, bioreactor, biomaterials, hydrogen fermentaion, tricalcium phosphate, nano risk, Instrumental analysis of cosmetics, synthetic mica, make-up cosmetic, powder engineering, UV defense, cosmetics, foundation, solar energy, cogeneration, biogas generation, reproduction energy, greenhouse gas, lack of oxygen, methane fermentation, multilayered cultivation, energy, biomass

Published Papers

  • The Effects of Growth Mwdium on the Yield and Components of Hydroponic Sweetpotatoes, Masaru Sakamoto, Takahiro Suzuki, Mem.Fac.B.O.S.T.Kindai Univ., Mem.Fac.B.O.S.T.Kindai Univ., 43, 29 - 38, Mar. 2020 , Refereed
  • Solubility evaluation og high absorbent calcium food made from scallop chellby artificial gastric juice test, Takahiro SUZUKI, Masaru SAKAMOTO, Nobuhiko TABATA, Yoshinari OKINO, Mem Fac Biol Oriented Sci Technol Kindai Univ, Mem Fac Biol Oriented Sci Technol Kindai Univ, 43, 1 - 20, Mar. 2020 , Refereed
  • Further investigation of recurrence mechanism of Fukushima offshore huge earthquake caused by global warming and study on the methane fermentation conditions from sweetpotatoes, Takahiro Suzuki, Masaru Sakamoto, JSES coference, JSES coference, Oct. 18 2019
    Summary:Since the sea level started rising due to global warming,
    strong earthquakes have frequently occurred in the
    Tohoku region. Last year, we found a high correlation
    between deep snow levels in Niigata and the number of
    earthquakes in Tohoku. Further investigations were
    conducted to explore the triggering factors, and it was
    found that strong spring and summer storms often caused
    huge earthquakes. Similarly, even in the case of the M9
    earthquake that occurred off the coast of Fukushima in
    March 2011, a significant pressure drop was observed in
    the Tohoku region. These results suggest that
    monitoring of climate change, snowfall and sea level can
    be useful in predicting some huge earthquakes.
    The results also alert that strong earthquakes and
    tsunami damage are repeated in short cycles unless fossil
    fuels, the main cause of global warming, are abolished.
    Therefore, in order to increase the production efficiency
    of biofuels to replace fossil fuels, we studied the
    conditions for methane fermentation from sweetpotatoes.
  • Methyl Jasmonate and Salinity Increase Anthocyanin Accumulation in Radish Sprouts, Masaru Sakamoto, Takahiro Suzuki, Horticulturae, Horticulturae, 5(3), 62/1 - 13, Sep. 2019 , Refereed
  • Elucidation of the huge earthquake occurrence mechanism by global warming and creation of fossil fuel alternative market by mass production of sweetpotato-methane, Takahiro Suzuki, Journal of Japan Solar Energy Society, Journal of Japan Solar Energy Society, 45(4), 31 - 37, Jul. 2019
  • Effect of Pot Volume on the Growth of Sweetpotato Cultivated in the New Hydroponis System, Masaru Sakamoto, Takahiro Suzuki, Sustainable Agriculture Research, Sustainable Agriculture Research, 7(1), 137 - 145, Jan. 2018 , Refereed
  • Comparison of Two Harvesting Methods for the Continuous Production of Indigo Plant (Polygonum Tinctorium) Leaves in a Closed-Cultivation System, Sakamoto M, Kim DA, Imoto K, Kitai Y, Suzuki T, Sustainable Agriculture Research, Sustainable Agriculture Research, 62(2), 7 - 12, May 2017 , Refereed
  • Effect of Root-Zone Temperature on the Growth and Fruit Quality of Hydroponically Grown Strawberry Plants, Sakamoto Masaru, Mayuka Uenishi, Kengo Miyamoto, Takahiro Suzuki, Journal of Agricultural Science, Journal of Agricultural Science, 8(5), 122 - 131, May 2016 , Refereed
  • Effect of Root-Zone Temperature on Growth and Quality of Hydroponically Grown Red Leaf Lettuce (Lactuca sativa L. cv. Red Wave), Masaru Sakamoto, Takahiro Suzuki, American Journal of Plant Sciences, American Journal of Plant Sciences, 6(14), 2350 - 2360, Sep. 2015 , Refereed
  • Elevated Root-Zone Temperature Modulates Growth and Quality of Hydroponically Grown Carrots, Masaru Sakamoto, Takahiro Suzuki, Agricultural Sciences, Agricultural Sciences, 6(8), 749 - 757, Aug. 2015 , Refereed
  • Effects of plant defense elicitors on anthocyanin accumulation in red baby leaf lettuce, SAKAMOTO MASARU, SUZUKI TAKAHIRO, Mem Fac Biol Oriented Sci Technol Kinki Univ, Mem Fac Biol Oriented Sci Technol Kinki Univ, (35), 1 - 6, Mar. 2015 , Refereed
  • A Survey of Environmental Radioactive Cesium Due to the Accident of TEPCO Fukushima Daiichi NPP in Kawamata-machi, Fukushima-ken, YAMANISHI Hirokuni, HORIBATA Akira, SUZUKI Takahiro, FURUKAWA Michio, INAGAKI Masayo, WAKABAYASHI Genichiro, HOHARA Sin-ya, ITOH Tetsuo, TANAKA Naomichi, ISHIWATA Shunji, TAGA Atsushi, OGATA Fumihiko, Journal of Smart Processing, Journal of Smart Processing, 4(6), 268 - 274, 2015
  • Biological and Systems Engineered Approach for Highly Efficient and High-value-added Production in Plant Factory System, 秋田 求, 鈴木 高広, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要, (26), 23 - 37, Sep. 2010 , Refereed
  • Influence of surface-adhered nanoparticles and nanoporous structure on particle-particle interaction of silica, Toshiyuki Kani, Maki Tamonoki, Takahiro Suzuki, Mayumi Tsukada, Hidehiro Kamiya, POWDER TECHNOLOGY, POWDER TECHNOLOGY, 176(2-3), 99 - 107, Jul. 2007 , Refereed
    Summary:In cosmetic powders, there are several kinds of powder materials widely used such as mica and silica on which nanoparticles are adhered on surface of the particle. However, it is not widely known that nanoparticles are adhered on the surface of these particles. In order to analyze the effect of these nanoparticles and silica particle nanopores on silica-mica surface interaction, the nanoparticles were removed by ultrasonic cleaning in water. To discuss the effect of the nanoporous structure of silica, nonporous silica of 5 pin mean particle diameter with adhered nanoparticles was used. The microscopic adhesion force between each silica particle and the mica surface before and after removal of the surface-adhered nanoparticles was characterized using colloid probe atomic force microscopy (AFM). It was observed that this force between nonporous silica and the mica plate increased by the removal of nanoparticles. In contrast, the adhesion force of porous silica slightly decreased on cleaning, and was almost independent of humidity after removal of the nanoparticles. Since water condensation in the nanopores of porous silica was promoted by the removal of surface nanoparticles, the formation of a liquid bridge between silica and mica was enhanced; thus, the adhesion force did not increase with humidity. (c) 2007 Elsevier B.V. All rights reserved.
  • Water Purification, Recycle and Improvement Eco-system on Polluted Water Area Using Effective Organisms and Materials., YOKOGAWA YOSHIYUKI, NISHIZAWA KAORI, NAGATA FUKUE, KATO KATSUYA, KAMEYAMA TETSUYA, SUZUKI TAKAHIRO, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 49(4), 223 - 232, Feb. 2001 , Refereed
  • 生体細胞との融合化を目指した高機能性セラミックス創製技術に関する研究(その2), 西澤 かおり, 鈴木 高広, 長江 肇, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 48(3), 245 - 258, Mar. 2000 , Refereed
  • Production of poly-beta-hydroxybutyric acid by microorganisms accumulated from river water using a two-stage perfusion culture system, T Morimoto, F Tashiro, H Nagashima, K Nishizawa, F Nagata, Y Yokogawa, T Suzuki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 89(1), 97 - 99, Jan. 2000 , Refereed
    Summary:The perfusion culture system using a shaken ceramic membrane flask (SCMF) was employed to accumulate microorganisms separated from river water and to produce poly-beta-hydroxybutyric acid (PHB). Using a two-step culture method with a single SCMF, river microorganisms were cultured by separately feeding four representative carbon sources, n-propanol, lactic acid, methanol, and formic acid. After 140 h culture, the cell concentration and PHB content respectively reached 43 g/l and 35% when a propanol medium was fed. Using a two-stage perfusion culture with twin SCMFs, the seed cell mass was increased in the first SCMF and then supplied to the second flask for PHB production. As a consequence, the cellular PHB content rose to 51% in the second SCMF, while the cell concentration gradually increased to 25 g/l after 175 h perfusion culture. These results demonstrated the utility of the two-stage perfusion culture system for developing a cheap means of producing PHB coincident with wastewater treatment.
  • Strategy for Diversification of Industrial Application of Lactic Acid Microorganism: Reports of the Research Groups for Fermentation Engineering on Lactic Acid Microorganism in 1998., OHASHI Ryo, YAMAOTO Tomonori, SUZUKI Takahiro, Seibutsu-kogaku Kaishi, Seibutsu-kogaku Kaishi, 77(11), 457 - 459, Nov. 1999
  • Research on the Preparation of Highly Functional Ceramics Aiming at Fusing Together with Living Cells. Part II., NISHIZAWA KAORI, SUZUKI TAKAHIRO, NAGAE HAJIME, KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, NAGATA FUKUE, TORIYAMA MOTOHIRO, KAMEYAMA TETSUYA, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 48(3), 245 - 258, Mar. 1999 , Refereed
  • SCMリアクターを用いた連続乳酸生産(乳酸菌の総合的産業利用に関する研究 : 平成9年度乳酸菌工学研究部会報告), 鈴木 高広, 大橋 亮, 山本 朋納, 生物工学会誌 : seibutsu-kogaku kaishi, 生物工学会誌 : seibutsu-kogaku kaishi, 76(11), 464 - 465, Nov. 1998
  • Surface modification of bioceramics by silane coupling agent and their evaluation, K Nishizawa, T Suzuki, Y Kawamoto, M Toriyama, Y Yokogawa, F Nagata, Y Tai, T Kameyama, JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 106(7), 709 - 714, Jul. 1998 , Refereed
    Summary:The surface of p-tricalcium phosphate ceramics (P-TCP) was modified by exposing ceramic disks to a silane coupling agent for different periods of time. The number of organic groups introduced was determined as a function of exposure time. These results suggest that zeta potential, cell adhesion and growth, and adsorbed protein concentration can carefully be controlled by silane treatment.
  • High-level expression of the methanol-inducible beta-galactosidase gene by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask, R Ohashi, E Mochizuki, Y Kamoshita, T Suzuki, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 86(1), 44 - 48, 1998 , Refereed
    Summary:The perfusion culture technique using a shaken ceramic membrane flask (SCM flask) was applied to achieve high-level expression of recombinant gene. A recombinant methylotrophic yeast strain, Pichia pastoris, was cultured aerobically with head space ventilation on a reciprocal shaker using an SCM flask. High-level production of beta-galactosidase was attempted by increasing both the cell concentration and the intracellular content of beta-galactosidase. The productivity and yield of beta-galactosidase were compared between two-stage culture and growth-associated production methods, In the two-stage culture method, the cell concentration was first raised to 57 g/l at 121h by feeding glycerol and, thereafter, expression of beta-galactosidase was induced by feeding methanol. The beta-galactosidase activity in the culture broth quickly increased up to 96 kU/ml within 48 h of initiating the production phase, while the cell concentration continued to increase, reaching 106 g/l after 167 h culture. On the other hand, in the growth-associated production method, beta-galactosidase was produced from an early stage of the culture by the feeding of methanol. The beta-galactosidase activity reached 152 kU/ml at 168 h, while the cell concentration was depressed to 49.7 g/l. The results showed that the growth-associated production method with the feeding of methanol was highly effective for high-level expression of the methanol-inducible recombinant gene of Pichia pastoris. A beta-galactosidase productivity level 10 times higher than that obtained in an ordinary fed-batch culture using a shake flask was readily achieved by continuous replenishment of the culture supernatant.
  • SCMリアクターを用いた高濃度乳酸菌の灌流培養技術(乳酸菌の総合的産業利用に関する研究 : 平成8年度乳酸菌工学研究部会報告), 鴨下 祐也, 大橋 亮, 高野 雅子, 内藤 友芽, 中 留美, 倉金 恵三, 仲野 徹, 森本 敏明, 山本 朋納, 鈴木 高広, 生物工学会誌 : seibutsu-kogaku kaishi, 生物工学会誌 : seibutsu-kogaku kaishi, 75(5), 373 - 375, Sep. 1997
  • 河川等の生物学的浄化システム用リサイクルセラミックス (生活環境関連材料特集), 横川善之, 鈴木高広, 長江 肇, 河本ゆかり, 西澤かおり, 永田夫久江, 鳥山素弘, 亀山哲也, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 46(4), 239 - 248, Apr. 1997 , Refereed
  • Effective control of glutamic acid fermentation by feeding of molasses using a pulse feed reaction method, YUYA KAMOSHITA, MICHIMASA KISHIMOTO, RYO OHASHI, TAKAHIRO SUZUKI, Proceedings of the 2nd Asian Control Conference, Proceedings of the 2nd Asian Control Conference, 2, 108 - 111, 1997
  • Control of filtering performance for dense cell retention culture of microorganisms using a stirred ceramic membrane reactor, TAKAHIRO SUZUKI, YUYA KAMOSHITA, RYO OHASHI, Proceedings of the 2nd Asian Control Conference, Proceedings of the 2nd Asian Control Conference, 2, 887 - 890, 1997
  • Synthesis of Calcium-Strontium, Calcium-Magnesium, Magnesium-Strontium Apatite Through Mechanochemical Method., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, MUCALO M R, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 45(4), 161 - 166, Apr. 1996 , Refereed
  • Surface Analysis of Calcium Phosphate Ceramics Modified with Silane Coupling Reagents, 西沢 かおり, 鳥山 素弘, 鈴木 高広, Reports of the National Industrial Research Institute of Nagoya, Reports of the National Industrial Research Institute of Nagoya, 45(4), 175 - 184, Mar. 1996 , Refereed
  • Morphology Control of the Hydroxyapatite Crystals under Hydrothermal Condition in the Presence of Methanol., NAGATA FUKUE, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 45(2), 95 - 103, Feb. 1996 , Refereed
  • Development of high-density culture system. (Ministry of Education S), SUZUKI TAKAHIRO, 乳酸発酵トータルシステムの開発と知識ベース自律分散制御システムの構築 平成7年度 No.06555249, 乳酸発酵トータルシステムの開発と知識ベース自律分散制御システムの構築 平成7年度 No.06555249, 1(1),1-23, 1996
  • Studies on Development of Biological Purification Systems Using Non-Reused Local Materials for the Improvement of Water Environment., INAMORI YUHEI, MIZUOCHI MOTOYUKI, NISHIMURA OSAMU, KAMEYAMA TETSUYA, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMOTO YUKARI, NISHIZAWA KAORI, TORIYAMA MOTOHIRO, 環境保全研究成果集, 環境保全研究成果集, 1995(Pt 2), 95.1-95.15, 1996
  • Studies on Development of Biological Purification Systems Using Non-Reused Local Materials for the Improvement of Water Environment., INAMORI YUHEI, MIZUOCHI MOTOYUKI, NISHIMURA OSAMU, KAMEYAMA TETSUYA, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMOTO YUKARI, NISHIZAWA KAORI, TORIYAMA MOTOHIRO, 都市における環境保全計画手法の開発に関する総合研究 平成7年度 騒音・振動の防止及び評価に関する総合研究 地域密着型環境研究, 都市における環境保全計画手法の開発に関する総合研究 平成7年度 騒音・振動の防止及び評価に関する総合研究 地域密着型環境研究, 95.1-95.15, 1996
  • Preparation of calcium phosphate compound-chitin fiber composite material, Y. YOKOGAWA, J.P. REYES, M.R. MUCALO, M.TORIYAMA, T. SUZUKI, K. NISHIZAWA, F. NAGATA, T. KAMEYAMA, Bioceramics, Bioceramics, 9, 427 - 430, 1996 , Refereed
  • Effects of ethylamine on the crystal growth of hydroxyapatite, F. NAGATA, Y. YOKOGAWA, M. TORIYAMA, Y. KAWAMOTO, T. SUZUKI, K. NISHIZAWA, T. KAMEYAMA, Phosphorus Research Bulletin, Phosphorus Research Bulletin, 6, 209 - 212, 1996 , Refereed
  • HYDROTHERMAL SYNTHESIS OF HYDROXYAPATITE CRYSTALS IN THE PRESENCE OF METHANOL, F NAGATA, Y YOKOGAWA, M TORIYAMA, Y KAWAMOTO, T SUZUKI, K NISHIZAWA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 103(1), 70 - 73, Jan. 1995 , Refereed
    Summary:The effect of methanol on the morphology of hydroxyapatite (HAp) crystals has been studied under hydrothermal conditions. A precursor HAp slurry prepared from CaCO3 and CaHPO4.2H4O was synthesized hydrothermally in the presence of methanol. The products obtained from slurries containing varying amounts of methanol were found to be carbonate-containing HAp by X-ray diffraction and IR spectrometry. TEM showed that the morphology of the products was influenced by the amount of methanol added to the slurry. The products obtained from slurries without methanol were rod-like or granular crystals, about 20 to 100 nm in size. Addition of methonal to the slurries caused an increase in the ratio of plate-like crystals to rod-like crystals or granular crystals. When the weight of methanol added was equal to the weight of the slurry, only plate-like crystals 20 to 200 nm in size were obtained. X-ray diffraction patterns of oriented products indicated that the plane grown selectively was the a-plane of the plate-like crystals. The results by DTA suggested that the variation of the morphology can be attributed to the adsorption of methanol on the surface of the HAp nuclei inhibiting the crystal growth along a particular axis.
  • WETTABILITY OF CALCIUM-PHOSPHATE CERAMICS BY WATER, M TORIYAMA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, K NISHIZAWA, F NAGATA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 103(1), 46 - 49, Jan. 1995 , Refereed
    Summary:To evaluate the wettability of phosphate ceramics by water, contact angles of hydroxyapatite-tricalcium phosphate composite ceramics were measured by the sessile drop and adhesion tension methods. It was difficult to obtain an equilibrium contact angle by the sessile drop method. On the other hand, reproducible values were obtained by the adhesion tension method if the sample was ground to less than 0.6 mm in thickness. The contact angles of hydroxyapatite and beta-tricalcium phosphate ceramics were 30.7-degrees and 23.5-degrees, respectively. The contact angle of the composite ceramics obtained by mixing of hydroxyapatite with tricalcium phosphate increased suggesting that the hydrophilicity of the surface decreased. The wettability of the composite ceramics increased with increasing surface hydroxyl group density measured by X-ray photoelectron spectroscopy.
  • Studies on Development of Biological Purification Systems Using Non-Reused Local Materials for the Improvement of Water Environment., INAMORI YUHEI, TAKAGI HIROO, MIZUOCHI MOTOYUKI, NISHIMURA OSAMU, SUZUKI TAKAHIRO, YOKOKAWA YOSHIYUKI, KAWAMOTO YUKARI, NISHIZAWA KAORI, TORIYAMA MOTOHIRO, 環境保全研究成果集, 環境保全研究成果集, 1994(Pt 2), 92.1-92.12, 1995
  • Nitric oxide synthase and cyclooxygenase-2 in pseudo-synovial membranes of loosened total hip prostheses, M. Hukkanen, J. Batten, I.D.McCarthy, T. SUZUKI, A.K.Aune, S.P.F.Hughes, J .M. Polak, Bone, Bone, 17(6), 582 - 582, 1995
  • Thermal Induced Change in Hydroxyapatite Crystals, F.NAGATA, Y.YOKOGAWA, M.TORIYAMA, Y. KAWAMOTO, T. SUZUKI, K. NISHIZAWA, M.R. MUCALO, Fourth Euro Ceramics, Fourth Euro Ceramics, 8, 125 - 131, 1995
  • Hydroxyapatite Coating on Alumina Single Crystal Substrate by Dip-Coating Technique., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, MORISHITA FUMIYASU, MUCALO M R, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 44(1), 1 - 7, Jan. 1995 , Refereed
  • Effects of the Surface Wettability and Zeta Potential of Calcium Phosphate Bioceramics on the Adhesion and Growth of Anchorage-Dependent Animal Cells., NISHIZAWA KAORI, TORIYAMA MOTOHIRO, SUZUKI TAKAHIRO, KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, NAGATA FUKUE, MUCALO M R, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 44(1), 8 - 14, Jan. 1995 , Refereed
  • SURFACE MODIFICATION OF CALCIUM-PHOSPHATE CERAMICS WITH SILANE COUPLING REAGENTS, K NISHIZAWA, M TORIYAMA, T SUZUKI, Y KAWAMOTO, Y YOKOGAWA, F NAGATA, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, 1995(1), 63 - 67, Jan. 1995 , Refereed
    Summary:Surface of calcium phosphate ceramics was modified with silane coupling reagents using reflux method, The results of thermal analyses and infrared and mass spectra suggested that surface hydroxy groups oi the ceramics form covalent bonds with silane coupling reagent molecules. Regardless oi the reagent species, almost the same number of organic groups was introduced when the reaction conditions were the same. The zeta potential of the modified ceramics varied with the number of the introduced organic groups. This result suggests that the cell compatibility oi the ceramics can be changed by controlling the amount of the introduced organic groups.
  • A multistat control system for cell retention culture using a stirred ceramic membrane reactor, TAKAHIRO SUZUKI, TAKESHI SATO, YASUYUKI, KADOTANI, MINORU KOMINAMI, Proceedings of the First Asian Control Conference, Proceedings of the First Asian Control Conference, 3, 535 - 538, 1994
  • A continuous culture system using a stirred ceramic membrane bioreactor, TAKAHIRO SUZUKI, TAKESHI SATO, YASUYUKI, KADOTANI, MINORU KOMINAMI, Proceedings of the Fifth International Symposium on Process System Engineering, Proceedings of the Fifth International Symposium on Process System Engineering, 1, 677 - 681, 1994
  • Production of Fermented Feed for Cattle from Stale Bread., SUZUKI TAKAHIRO, SATO TAKESHI, KADOTANI YASUYUKI, KOMINAMI MINORU, SHINKE RYU, 名古屋工業技術研究所報告, 名古屋工業技術研究所報告, 42(12), p337 - 345, Dec. 1993 , Refereed
  • Bioactive Ceramics Coating. (IV). .BETA.-Tricalcium Phosphate Coating on Alumina Substrate by the Use of Anorthite as an Interlayer., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 42(4/5), 123 - 133, Apr. 1993 , Refereed
  • Bioactive Ceramics Coating. (V). .BETA.-Tricalcium Phosphate Coating on Titanium Substrate by the Use of Crystallized Glass., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 42(4/5), 134 - 140, Apr. 1993 , Refereed
  • Bioactive Ceramics Coating. (III). Apatite Coating on an Yttria Partially Stabilized Zirconia Plate in the Presence of Water Vapor., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 42(4/5), 117 - 122, Apr. 1993 , Refereed
  • Bioactive Ceramics Coating. (II). Coating of Zirconia with Tricalcium Phosphate., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 42(4/5), 109 - 116, Apr. 1993 , Refereed
  • Bioactive Ceramics Coating (I) Coating of .BETA.-Tricalcium Phosphate onZirconia Using Magnesium Metaphosphate., KAWAMOTO YUKARI, TORIYAMA MOTOHIRO, YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 42(2/3), 64 - 70, Feb. 1993 , Refereed
  • PREPARATION AND EVALUATION OF MULLITE CERAMICS CARRIER FOR IMMOBILIZATION OF ENZYME, M TORIYAMA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, K NISHIZAWA, H NAGAE, M KATO, T FUKUSHIMA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 100(11), 1376 - 1380, Nov. 1992 , Refereed
    Summary:Carrier functions of nine kinds of oxide ceramics for immobilization of enzymes have been examined. Mullite ceramics were the most suitable material for the immobilization of enzyme and stabilization of immobilized enzyme activity. Control of the pore structure and SiO2/Al2O3 ratio of mullite ceramics was carried out for improving the efficiency of carrier for immobilization of enzymes. Immobilized enzyme activity was highest for the stoichiometric mullite sintered at 1400-degrees-C, which was formed by adding liquid paraffin to the mixed SiO2/Al2O3 powder prepared from colloidal sol.
  • SYNTHESIS OF HYDROXYAPATITE BY AN OXIDATIVE DECOMPOSITION METHOD OF CALCIUM CHELATE, M TORIYAMA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, K NISHIZAWA, H NAGAE, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 100(7), 950 - 954, Jul. 1992 , Refereed
    Summary:Preparation of hydroxyapatite (Hap) was investigated by a homogeneous precipitation technique. The precipitates were obtained by mixing solutions of Ca (NO3)2, (NH4)2HPO4, EDTA-Na and H2O2 at 90-degrees-C. The products were identified as carbonate-containing hydroxyapatite by the X-ray diffraction and IR spectrometry. When pH value of the reacting solution was higher than 10, Ca/P molar ratio of the precipitate was 1.67. Synthesized Hap powders by this method had specific surface area of 5-35 m2/g, and the Hap particles showed clear hexagonal pyramidal ideomorphic shapes.
  • APATITE COATING ON YTTRIA DOPED PARTIALLY-STABILIZED ZIRCONIA PLATE IN THE PRESENCE OF WATER-VAPOR, Y YOKOGAWA, M TORIYAMA, Y KAWAMOTO, T SUZUKI, S KAWAMURA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 100(4), 602 - 604, Apr. 1992 , Refereed
    Summary:A new method of apatite coating on yttria partially stabilized zirconia plate was studied. Firstly, calcium metaphosphate was heated and fused onto 3 mol% yttria partially stabilized zirconia plate to form an interlayer. Then a mixture of calcium metaphosphate and tetracalcium phosphate was applied on the zirconia plate, followed by heating in the presence of water vapor. Apatite and tricalcium phosphate formed on the surface of the zirconia plate by heating at 1100-degrees-C while calcium pyrophosphate and tricalcium phosphate but apatite formed above 1200-degrees-C. The products of the coated layer were discussed in relation to the reaction at the interface between zirconia plate and coated layer.
  • Preparation of hydroxyapatite-tricalcium phosphate composite coating and its biological characterization, M. TSUZUKI, K. KONDO, Y. MATSUNO, T. SUZUKI, S. KAWAMURA, Apatite, Apatite, 1(1), 171 - 174, 1992 , Refereed
  • Improved parameter estimation in fed-batch fermentation through reliability filtering, TAKAHIRO SUZUKI, JOHN J. PRIOR, CHARLES L. COONEY, Proceedings of International Symposium on Advanced Computing for Life-Science, Proceedings of International Symposium on Advanced Computing for Life-Science, 1, 284 - 286, 1992
  • BETA-TRICALCIUM PHOSPHATE COATING ON TITANIUM, M TORIYAMA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, K NISHIZAWA, H NAGAE, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 99(12), 1268 - 1270, Dec. 1991 , Refereed
    Summary:Bioactive beta-tricalcium phosphate (beta-TCP) coating onto titanium was studied. It is shown that the beta-TCP adhered to the surface of a titanium plate by the use of a frit, which was composed of CaO, TiO2, P2O5 and a few mol% Na2O. The frit as a coated layer resulted in the formation of beta-TCP and CaTi4 (PO4)6 phases by a thermal treatment at 800-degrees-C for 24 h.
  • Biocompatible ceramic porous body with thrombin solubility., SUZUKI TAKAHIRO, TORIYAMA MOTOHIRO, ファインセラミックス, ファインセラミックス, 12(3), 127 - 133, May 1991
  • TRICALIUM PHOSPHATE COATING ON ZIRCONIA BY USING CALCIUM METAPHOSPHATE AND TETRACALCIUM PHOSPHATE, Y YOKOGAWA, Y KAWAMOTO, M TORIYAMA, T SUZUKI, S KAWAMURA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 99(3), 211 - 214, Mar. 1991 , Refereed
    Summary:beta-tricalcium phosphate (beta-TCP) coating on yttria partially stabilized zirconia plate was studied. Firstly, calcium metaphosphate (CaP2O6) layer was coated on yttria partially stabilized zirconia as an interlayer by heating at 1000-degrees-C for 40 min. Then, a mixed slurry of calcium metaphosphate and tetracalcium phosphate (Ca4(PO4)2O) was brought into reaction on the zirconia plate by heating at 1200-degrees-C for 40 min. The coated layer was found to be beta-tricalcium phosphate with thickness of about 100-mu-m.
  • PREPARATION OF TRIMAGNESIUM PHOSPHATE BY MECHANOCHEMICAL REACTION, Y YOKOGAWA, Y KAWAMOTO, M TORIYAMA, T SUZUKI, S KAWAMURA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 99(2), 150 - 152, Feb. 1991 , Refereed
    Summary:Trimagnesium phosphate hydrate was synthesized by mechanochemical reaction of a slurry of magnesium hydrogen phosphate trihydrate and magnesium oxide with the molar ratio of 2 : 1 in a pot mill for a few hours. Mg3 (PO4)2.8H2O was obtained by using a slurry with more than 60 wt% water; Mg3 (PO4)2.22H2O, with 40 wt% water. The anhydrate of trimagnesium phosphate was obtained by heating Mg3 (PO4)2.8H2O or Mg3 (PO4)2.22H2O above 700-degrees-C.
  • COATING OF BETA-TRICALCIUM PHOSPHATE ON YTTRIA-PARTIALLY STABILIZED ZIRCONIA USING MAGNESIUM METAPHOSPHATE AS AN INTERLAYER, Y KAWAMOTO, Y YOKOGAWA, M TORIYAMA, S KAWAMURA, T SUZUKI, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 99(1), 19 - 22, Jan. 1991 , Refereed
    Summary:Yttria-partially stabilized zirconia (Y-PSZ) coated with beta-tricalcium phosphate (beta-TCP) was studied for developing high-strength biomaterial. Y-PSZ substrate was first coated with a slurry of magnesium metaphosphate used as an interlayer and heated at 1120-degrees to 1240-degrees-C. Then the product was coated with a slurry of beta-TCP and heated at 1100-degrees to 1220-degrees-C. The obtained coatings bonded well to the substrate. The beta-TCP phase was retained in the coating layer of all samples. A tetragonal-to-monoclinic destabilization of the Y-PSZ was observed in the reaction layer.
  • Bioaffinity test on ceramic materials for medical use by tissue culture method., SUZUKI TAKAHIRO, TORIYAMA MOTOHIRO, KAWAMURA SUKEZO, KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(11), p444 - 452, Nov. 1990 , Refereed
  • High strength .BETA.-tricalcium phosphate ceramics. II. Sintering behavior of .BETA.-tricalcium phosphate., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMURA SUKEZO, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(5), 224 - 228, May 1990 , Refereed
  • High strength .BETA.-tricalcium phosphate ceramics. III. Inhibitive effect of additives in phase transformation., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMURA SUKEZO, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(5), 229 - 234, May 1990 , Refereed
  • High strength .BETA.-tricalcium phosphate ceramics. IV. Effect of mixed addition of alumina and silica., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMURA SUKEZO, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(5), 235 - 240, May 1990 , Refereed
  • High strength .BETA.-tricalcium phosphate ceramics. V. Estimation of biocompatibility by a tissue culture method., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMURA SUKEZO, EBIHARA SHIGEYOSHI, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(5), 241 - 246, May 1990 , Refereed
  • High strength .BETA.-tricalcium phosphate ceramics. I. Mechanochemical synthesis of .BETA.-tricalcium phosphate., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, KAWAMURA SUKEZO, 名古屋工業技術試験所報告, 名古屋工業技術試験所報告, 39(5), 217 - 223, May 1990 , Refereed
  • ESTIMATION OF BIOCOMPATIBILITY OF HIGH-STRENGTH BETA-TRICALCIUM PHOSPHATE CERAMICS BY A TISSUE-CULTURE METHOD, M TORIYAMA, S KAWAMURA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, S EBIHARA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 98(4), 404 - 407, 1990 , Refereed
    Summary:Three kinds of high strength β-tricalcium phosphate (β-TCP) ceramics (β-TCP ceramic, β-TCP ceramic with solute magnesium content, and β-TCP-AIPO_4 composite ceramic) were prepared. Their biocompatibility was tested by a tissue culture method by use of a line cell derived from Chinese hamster (V 79). Initial cell attachment of β-TCP ceramics was smaller than that of polystyrene tissue culture dish, because the surface of β-TCP ceramics was too
  • BETA-TRICALCIUM PHOSPHATE COATING ON ALUMINA CERAMIC, M TORIYAMA, S KAWAMURA, Y KAWAMOTO, T SUZUKI, Y YOKOGAWA, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, NIPPON SERAMIKKUSU KYOKAI GAKUJUTSU RONBUNSHI-JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, 98(9), 1054 - 1057, 1990 , Refereed
    Summary:A bioactive β-tricalcium phosphate (p-TCP) coating onto alumina ceramic was studied. It is shown that the β-TCP adhered to the surface of an alumina ceramic by the use of anorthite, which was formed by a thermal treatment of a mixture ofβ-TCP, alumina and silica, as an intermediate layer.
  • Mass production of intracellular metabolite by fully automatic fed-batch culture of microorganism, TSUNEO YAMANE, TAKAHIRO SUZUKI, SHOICHI SHIMIZU, Bioproducts and Bioprocesses, Bioproducts and Bioprocesses, 331 - 336, 1989 , Refereed
  • Pseudomonas fluorescensの流加培養によるリパーゼの生産(リパーゼの生産研究), 山根 恒夫, 鈴木 高広, 虫賀 義尚, 石原 一人, 清水 祥一, 醗酵工学会誌 : hakkokogaku kaishi, 醗酵工学会誌 : hakkokogaku kaishi, 66(5), 413 - 416, Sep. 1988
  • CONTROL OF MOLECULAR-WEIGHT OF POLY-BETA-HYDROXYBUTYRIC ACID PRODUCED IN FET-BATCH CULTURE OF PROTOMONAS-EXTORQUENS, T SUZUKI, H DEGUCHI, T YAMANE, S SHIMIZU, K GEKKO, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 27(5-6), 487 - 491, Feb. 1988 , Refereed
  • MASS-PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID BY FED-BATCH CULTURE WITH CONTROLLED CARBON NITROGEN FEEDING, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 24(5), 370 - 374, Aug. 1986 , Refereed
  • KINETICS AND EFFECT OF NITROGEN-SOURCE FEEDING ON PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID BY FED-BATCH CULTURE, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 24(5), 366 - 369, Aug. 1986 , Refereed
  • MASS-PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID BY FULLY-AUTOMATIC FED-BATCH CULTURE OF METHYLOTROPH, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 23(5), 322 - 329, Feb. 1986 , Refereed
  • Effect of Partial Excision of Early Taproots on Growth and Components of Hydroponic Carrots, Masaru Sakamoto, Masanori Wada, Takahiro Suzuki, Horticulturae, Horticulturae, 6(1), 5 - 12, Jan. 2020 , Refereed
  • Influence of surface-adhered nanoparticles and nanoporous structure on bulk flowability of silica, Toshiyuki Kani, Takahiro Suzuki, Mayumi Tsukada, Hidehiro Kamiya, POWDER TECHNOLOGY, POWDER TECHNOLOGY, 176(2-3), 108 - 113, Jul. 2007 , Refereed
    Summary:Nanoporous spherical silica particles with nanoparticles adhered to their surfaces and mica powder are generally used in cosmetic powders. To analyze the effect of surface-adhered nanoparticles and nanopores on bulk flowability, nanoparticles adhered to silica surfaces were removed by ultrasonic cleaning using water. To investigate the influence of the nanoporous structure of silica, nonporous silica (with a mean particle diameter of 5 mu m) with adhered nanoparticles was used. The bulk flowabilities of the mixtures of mica and silica powder with and without surface-adhered nanoparticles were characterized by using a friction tester and texture analyzer. The coefficient of dynamic friction (CDF) decreased for both the mixed powders due to the removal of nanoparticles. On the other hand, the aggregation strength (AS) increased due to the cleaning of nanoparticles. (c) 2007 Elsevier B.V. All rights reserved.
  • INFLUENCE OF NANOPOROUS STRUCTURE ON SILANE COUPLING SURFACE MODIFICATION BEHAVIOR AND ADHESION PROPERTIES OF SPHERICAL SILICA PARTICLES, Toshiyuki Kani, Maki Tamonoki, Takahiro Suzuki, Mayumi Tsukada, Hidehiro Kamiya, CHARACTERIZATION AND CONTROL OF INTERFACES FOR HIGH QUALITY ADVANCED MATERIALS II, CHARACTERIZATION AND CONTROL OF INTERFACES FOR HIGH QUALITY ADVANCED MATERIALS II, 198, 237 - +, 2007 , Refereed
    Summary:In cosmetic powder, there are several kinds of powder materials widely used, such as mica and porous silica on which nanoparticles adhered on the surface of particle. However, ills not widely known that nanoparticles are adhered on the surface of particle. In order to analyze the effect of a nanoporous structure on the surface modification behavior by using a silane coupling agent and the surface interaction between silica and mica, four types of silane coupling agents with different molecular structures were used for the surface treatment of spherical silica with and without a nanoporous structure. The microscopic adhesion force between each surface-modified silica particle and mica surface was characterized by using colloid probe atomic force microscopy (AFM). The adhesion force between the nonporous silica particles and mica surface was increased by the surface modification using almost all silane coupling agents. In contrast, the adhesion force between nanoporous silica particles and mica was decreased by the surface modification. Since the amount of reacted silane coupling agent per unit surface area of nanoporous silica particles was much less than that of nonporous silica particles.
  • A shaking bioreactor equipped with twin ceramic membranes for acetic acid production using Acetobacter pasteurianus, J Horiuchi, M Narumi, K Tada, M Kobayashi, T Kanno, T Suzuki, BIOTECHNOLOGY LETTERS, BIOTECHNOLOGY LETTERS, 24(23), 1987 - 1991, Dec. 2002 , Refereed
    Summary:A shaking bioreactor system with twin internal ceramic membranes was developed for effective perfusion culture and applied to the continuous production of acetic acid using Acetobacter pasteurianus. The system makes it possible to carry out the back-washing of the membrane without stopping the continuous operation because one membrane can be washed by medium feed flow while another membrane provides filtration of the broth by the simple switching of the medium and the broth flow direction. The medium flow through the membrane could successfully wash the surface of the membrane thereby effectively maintaining the filtration ability. By using the system, continuous operation of more than 800 h was achieved and the maximum acetic acid productivity reached 13.4 g l(-1) h(-1) using air enriched with 40% O-2.
  • Growth and adhesion of osteoblast-like cells derived from neonatal rat calvaria on calcium phosphate ceramics, T Suzuki, M Hukkanen, R Ohashi, Y Yokogawa, K Nishizawa, F Nagata, L Buttery, J Polak, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 89(1), 18 - 26, Jan. 2000 , Refereed
    Summary:The effects of biocompatible ceramics on the growth and adhesion of osteoblast-rich rat calvarial cell cultures were investigated. Osteoblast-like cells and mouse fibroblast-like L-929 cells were cultured on composite sinters of hydroxyapatite (HAP) and beta-tricalcium phosphate (TCP) culture carriers, whose Ca/P molar ratios were adjusted to values of 1.50, 1.55, 1.60, 1.64 and 1.67. The growth rates of both. cell types were accelerated on the TCP-HAP ceramics as compared to those on polystyrene plastic (LUX) or bioinert zirconia ceramics. The population of osteoblast-like cells reached a density of 2.28 x 10(5) cells/cm(2) on 100% HAP (Ca/P ratio 1.67) at 9 d of culture, while the corresponding cell density was 1.66 x 10(5) cells/cm(2) on LUX and 1.26 x 10(5) cells/cm(2) on zirconia. Adhesion of the osteoblast-like cells on TCP-HAP ceramics was similarly increased as compared with that on LUX or zirconia ceramics. The adhesion of L-929 cells on TCP-HAP ceramics was found to be weaker than that on cultures on LUX or zirconia ceramics. The time-dependent variations in the alkaline phosphatase activity of the osteoblast-like cells showed that the osteoblastic phenotype was potentiated by culturing the cells in calcium-rich media. The surface analyses of the Ca/P ratio and the microstructure by XRD and FTIR suggest that the Ca-rich surface was newly formed by recombination on the surface layer in the culture medium containing fetal bovine serum. These results suggest that the surface of TCP-HAP ceramics, especially that of 100% HAP ceramics, are effective for accelerating growth and differentiation of osteoblast-like cells. This is most probably due to the chemical and physical instability and composition of 100% HAP, which promote the formation of a Ca-rich layer at the cell-material interface and provision of Ca ions to the osteoblast-like cells.
  • A mini-scale mass production and separation system for secretory heterologous proteins by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask, R Ohashi, E Mochizuki, T Suzuki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 87(5), 655 - 660, May 1999 , Refereed
    Summary:The perfusion culture technique using a shaken ceramic membrane flask (SCM flask) was applied to the production of a secretory heterologous protein. A recombinant methylotrophic yeast strain, Pichia pastoris, was cultured aerobically on a reciprocal shaker using an SCM flask. High-level production of human serum albumin (HSA) was attempted by increasing both the cell concentration and the expression level of the recombinant gene. In the two-stage culture method, the cell concentration was first raised to 17 g/l by feeding glycerol, after which the expression of HSA was induced by feeding methanol. However, the concentration of HSA in the effluent filtrate was as low as 0.15 g/l, while the cell concentration continued to increase. In contrast, HSA was effectively produced by feeding methanol from an early stage of the culture. In this case, the HSA concentration reached 0.24 and 0.46 g/l, respectively, using the growth-associated production method without and with aeration into the head space of the SCM flask. The results showed that supplying sufficient oxygen together with the growth-associated induction method are effective for obtaining high-level expression of the methanol-inducible recombinant gene of P. pastoris. An HSA concentration in the filtrate of 1.5 g/l was finally achieved when the cell concentration was increased to 53 g/l by supplying oxygen-enriched gas to the SCM flask. The yield and productivity of HSA reached 2.6-fold and 10-fold those obtained in an ordinary fed-batch Culture using a shake flask, and these levels were readily achieved by continuous replenishment of the culture supernatant. The achievements made in this study should contribute to the development of a handy bioreactor system for mini-scale mass production of target proteins with separation at high purity.
  • Continuous production of lactic acid from molasses by perfusion culture of Lactococcus lactis using a stirred ceramic membrane reactor, R Ohashi, T Yamamoto, T Suzuki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 87(5), 647 - 654, May 1999 , Refereed
    Summary:A perfusion culture system was used for continuous production of lactic acid by retaining cells at a high density of Lactococcus lactis in a stirred ceramic membrane reactor (SCMR). After the cell concentration increased to 248 g/l, half of the culture broth volume was replaced with the fermentation medium. Subsequently, a substrate solution containing glucose (run 1) or molasses (run 2) was continuously supplied to the cells retained in the SCMR. Simultaneously, the culture supernatant was extracted using a ceramic filter with a pore size of 0.2 mu m. The dilution rate was initially set at 0.4 h(-1) and gradually decreased to 0.2 h(-1) due to reduction in the permeability of the filter. The concentration of glucose in the substrate solution was adjusted to 60 g/l for the transition and the first period until 240 h, 90 g/l for the second period from 240 h to 440 h, and 70 g/l for the third period from 440 h to 643 h. The average concentration of lactic acid in the filtrate reached 46 g/l in the first period, 43 g/l in the second period, and 33 g/l for the third period. The productivity obtained for the first period reached 15.8 g.l(-1).h(-1), twice as much as that achieved in repeated batch fermentations. Based on the results obtained in run 1, the substrate solution containing 120 g/l of molasses was continuously supplied for 240 h in run 2. The concentration and productivity of lactic acid reached 40 g/l and 10.6 g.l(-1).h(-1), respectively, by continuously replenishing the culture medium at a dilution rate of 0.26 h(-1). These results demonstrated that the filtration capacity of the SCMR was sufficient for a continuous and rapid replenishment of molasses solution from the dense cell culture and, therefore, the perfusion culture system is considered to provide a low-cost process for continuous production of lactic acid from cheap resources.
  • Initial anchoring and proliferation of fibroblast L-929 cells on unstable surface of calcium phosphate ceramics, T Suzuki, R Ohashi, Y Yokogawa, K Nishizawa, F Nagata, Y Kawamoto, T Kameyama, M Toriyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 87(3), 320 - 327, Mar. 1999 , Refereed
    Summary:Calcium phosphate ceramics constructed from beta-tricalcium phosphate (TCP) and hydroxyapatite (HAP) have been successfully used as implant materials. However, there is a possibility that these materials are responsible for an unwanted inflammatory response during wound healing. Since TCP is soluble in the body, the instability of this material may contribute to this inflammatory response. Using composite ceramics of TCP and HAP that possessed Ca/P molar ratios of 1.50, 1.55, 1.60, 1.64, and 1.67, the effect of surface instability on fibroblast L-929 cells was investigated. The time-dependent variation of the initial anchoring ratio, cell density, and cell viability were measured. In general, the cells were severely damaged and ruptured on the highly soluble thin surface layer of the TCP-HAP ceramics. The initial anchoring ratio for TCP-HAP ceramics was as high as that for the polystyrene dish (Lux, control). However, viability at 6 h decreased to less than 50% of the initial cell density on ceramics with a Ca/P molar ratio of 1.64 (20% TCP-80% MAP), while 85% of the cells were viable on Lux. The viability on 100% TCP, whose surface is the most highly soluble among the TCP-HAP ceramics used in this study, was reduced to 20%. Morphological observation showed that the anchored cells were ruptured when grown in culture medium on the 100% TCP. Although the high solubility of the thin surface layer on the TCP-HAP ceramics of the carrier was found to be the dominant factor in the decreasing cell viability, the initial viability was enhanced by the stabilization of the surface of the TCP-HAP ceramics by pre-incubating the scaffolds in a culture medium containing 10% fetal bovine serum for 3 d.
  • Further biocompatibility testing of silica-chitosan complex membrane in the production of tissue plasminogen activator by epithelial and fibroblast cells, Takahiro Suzuki, Yasuyuki Mizushima, Tomohiro Umeda, Ryo Ohashi, Journal of Bioscience and Bioengineering, Journal of Bioscience and Bioengineering, 88(2), 194 - 199, 1999 , Refereed
    Summary:The effects of the physicochemical characteristics of a silica-chitosan complex membrane (SiCM) on the expression of tissue plasminogen activator (tPA) by contacting cells were investigated with the aim of improving the biocompatibility of the novel implant biomaterial. Expression of tPA is considered to be effective in wound healing by preventing thrombus formation, which causes inflammatory responses and rejection of implant materials. Inducing the epithelial cells surrounding implant materials to secrete tPA, which serves as an early signaling system to proliferate cells underlying connective tissues, would be further effective in accelerating wound healing. An epithelial 293 cell line derived from human embryonic kidney and a fibroblast IMR-90 cell line from human lung possessing the ability to secrete tPA were cultured on SiCMs, whose composition was stepwise controlled by adjusting the mixing ratio between silica and chitosan to give silica contents of 20, 33, 43, and 50wt%. Both strains showed strong adhesion on chitosan (0%-SiCM) and 50%-SiCM. The cell proliferation rates were also accelerated in a manner that was dependent on the increase in the adhesion strength of the cells cultured on the SiCMs. Furthermore, the tPA activity in the culture medium increased in accordance with the cell density, while the cellular specific activity of IMR-90 cells to secrete tPA was synergistically enhanced by strong adhesion and a high cell density on the surface of chitosan and 50%-SiCM. Analysis of the physicochemical effects of the SiCMs revealed that the cells were dominantly affected by the surface hydrophobicity rather than by the zeta potential, as well as by the mixing ratio between chitosan and silica. The wet contact angles of 50%-SiCM and chitosan, which were 68°and 65°, respectively, were found to be suitable for adhesion and growth of both the epithelial 293 cells and fibroblast IMR- 90 cells. A hydrophobic surface at 65°-68°was also effective for the production of tPA by IMR-90 cells, whereas the tPA activity of 293 cells reached its highest level on the SiCM with a wet contact angle of 63°. These results suggest that a suitable adhesion strength is a significant factor in the expression of tPA by cells contacting an implant biomaterial.
  • A dense cell culture system for aerobic microorganisms using a shaken ceramic membrane flask with surface aeration, Y Kamoshita, R Ohashi, T Suzuki, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 85(2), 218 - 222, 1998 , Refereed
    Summary:Aeration of the head space in a shaken ceramic membrane flask (SCM flask) capped with a cotton plug was found to be essential for increasing cell concentration and viability of aerobic microorganisms. Ventilation through the cotton plug cap was insufficient for satisfying the oxygen demand of aerobically growing tells in the SCM flask. Dissolved oxygen concentration (DO) in the culture supernatant of Escherichia coli in batch culture using the SCM flask dropped to nearly 0 ppm when the cell concentration reached 1 g/l, while CO2 concentration in the head space of the SCM flask increased quickly to nearly 20% owing to accumulation of the evolved CO2. In contrast, the oxygen transfer coefficient in a SCM flask shaken at 230 rpm was as high as 220 h(-1), compared to that in a stirred type jar fermenter. Consequently, aeration of the head space of the SCM flask was found to be sufficient for supplying oxygen by vigorous waves created in the culture broth due to the reciprocal shaking action. Using the SCM flask, E. coli cell mass reached 84 g/l in 40 h with aeration of the head space with oxygen-enriched gas. The SCM flask with aeration to the head space enabled an increase in the concentration and productivity of viable cells to be readily achieved by continuously replenishing the culture supernatant with oxygen in conjunction with the removal of the evolved CO2.
  • Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis, Y Kamoshita, R Ohashi, T Suzuki, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 85(4), 422 - 427, 1998 , Refereed
    Summary:A ceramic filter was fitted in a stirred ceramic membrane reactor (SCMR) for both extraction of culture supernatant and feeding of distilled water in reverse flow. The dependence of filtration performance on the cell concentration was decreased by about 20% by regularly cleaning the filter using a membrane cleaning system. The improved permeability effected an increase of both the growth rate and viability of Lactococcus lactis by increasing the dilution rate of the culture supernatant. Using the improved SCMR system, a cell concentration of 178 g/l and viability of 98% were obtained after 198 h of culture, while it took 238 h to obtain a cell concentration of 141 g/l and 94% viability without the use of the membrane cleaning system. The perfusion culture system was applied to the rapid batch fermentation of lactic acid by retaining cells at a high density in the SCMR. When the cell concentration reached 80 g/l, the culture supernatant was extracted and replaced with the fermentation medium, Batch fermentation using the retained cells was repeated six times. The concentration of lactic acid increased to more than 30 g/l within 2 h in each fermentation, while 1.2 h was necessary for replacing the culture supernatant to repeat the batch fermentation. The production rate of lactic acid was increased in proportion to the cell concentration, and a high fermentation activity of the retained cells was maintained via the repeated batch fermentation. These results demonstrate that the improved permeability of the SCMR with use of a membrane cleaning system effected a rapid increase in the concentration and viability of cells, and accordingly, the increased production rate of lactic acid in proportion to the concentration of viable cells.
  • Continuous production and separation of ethanol without effluence of wastewater using a distiller integrated SCM-reactor system, R Ohashi, Y Kamoshita, M Kishimoto, T Suzuki, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 86(2), 220 - 225, 1998 , Refereed
    Summary:Continuous ethanol production without effluence of wastewater was carried oat using a closed circulation system which integrated a cell retention culture system and a distillation system to separate ethanol. The stirred ceramic membrane reactor (SCMR), a jar fermenter fitted with asymmetric porous alumina ceramic membrane rods, was used for retaining high density of cells and extraction of the culture supernatant that was continuously sent to the distiller to evaporate ethanol. After the distillation process, the residual solation of the culture supernatant was returned to the SCMR via a heat exchanger. Two types of strains of Saccharomyces cerevisiae; a flocculating IR2 strain and a sake-brewing K901 strain, were cultured using the integrated fermentation system for continuous production of ethanol, When the ethanol concentration reached 60 g/l, the culture supernatant was extracted by filtration from the SCMR and sent to the distiller. During the repeated ethanol fermentation and recycling of the medium, cell concentration of K901 strain was increased to 236 g/l and the productivity of ethanol reached 13.1 g.l(-1).h(-1), which was higher than the productivity of 12.5 g.l(-1).h(-1) achieved by immobilized cells on calcium alginate beads (CAB). In addition, the productivity was further increased to 14.5 g.l(-1).h(-1) by immobilized K901 cells on CAB retained in the SCMR. In contrast, the flocculating IR2 strain was found to accumulate into crusts of cells on the surface of the ceramic filter and thereby the productivity of ethanol considerably decreased because of the reduced filtration performance. These results showed that the long-lasting and high filtration performance was essential for the increase in the productivity of ethanol using the integrated system that consisted of the SCMR and the distiller.
  • Simple and economized technique for a dense cell culture of microorganisms, R Ohashi, T Suzuki, COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998, COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998, 31(8), 185 - 188, 1998 , Refereed
    Summary:Feed rate of multiple substrates containing glucose, polypeptone and yeast extract was readily optimized for a high density cell culture using a multiple perfusion culture system. Three independent perfusion cultures of Lactococcus lactis were carried out simultaneously using shaken ceramic membrane flasks. Stepwise control of substrate feed rate was found to be the most effective in enhancing the cell growth and in reducing both the dilution rate and the total volume of the substrate solution. This optimized control strategy was applied to a scale-up culture system that employed a stirred ceramic membrane reactor. Copyright (C) 1998 IFAC.
  • Growth of calcium phosphate on phosphorylated chitin fibres, Y. Yokogawa, J. Paz Reyes, M. R. Mucalo, M. Toriyama, Y. Kawamoto, T. Suzuki, K. Nishizawa, F. Nagata, T. Kamayama, Journal of Materials Science: Materials in Medicine, Journal of Materials Science: Materials in Medicine, 8(7), 407 - 412, Jul. 1997 , Refereed
    Summary:Calcium phosphate growth on chitin phosphorylated fibres was studied using scanning electron microscopy and energy dispersive X-ray analysis (SEM, EDX), micro-Fourier transform infrared spectroscopy (FTIR), and solid state magic angle spinning nuclear magnetic resonance (MAS NMR) techniques. The C6 chemical shift positions of 13C MAS NMR in the chitin fibres phosphorylated using urea and H3PO4 are obvious indicating that phosphorylation takes place not in the C1 but in the C6 region. Micro-FTIR and 31P MAS NMR suggested that ammonium hydrogen phosphate formed during the phosphorylation procedure. Chitin fibres phosphorylated using urea and H3PO4 and then soaked in saturated Ca(OH)2 solution at ambient temperature, which lead to the formation of thin coatings formed by partial hydrolysis of the PO4 functionalities, were found to stimulate the growth of a calcium phosphate coating on their surfaces after soaking in 1.5 x SBF solution for as little as one day. The thin layer after Ca(OH)2 treatment functioned as a nucleation layer for further calcium phosphate deposition after soaking in 1.5 x SBF solution. EDX-measured Ca:P ratios of the coatings of Ca(OH)2-treated phosphorylated chitin in 1.5 x SBF solution suggested that calcium-deficient apatite was formed.
  • Surface instability of calcium phosphate ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells, T Suzuki, T Yamamoto, M Toriyama, K Nishizawa, Y Yokogawa, MR Mucalo, Y Kawamoto, F Nagata, T Kameyama, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 34(4), 507 - 517, Mar. 1997 , Refereed
    Summary:The surface of biocompatible ceramics made of synthesized hydroxyapatite (HAP) and p-tricalcium phosphate (TCP) was found to be extremely active in tissue culture medium. Using mixed ceramics of HAP and TCP which had been prepared with different Ca/P molar ratios adjusted in stepwise fashion to values of 1.50, 1.55, 1.60, 1.64 and 1.67, the characteristics of the surface were investigated. The time-dependent variation of zeta potential of the TCP-HAP ceramics immersed in distilled water and in culture medium with and without addition of fetal bovine serum showed that the surface was unstable with significant changes in the charge being observed. Dry TCP powder had a zeta potential of -19 mV, which shifted to -7 mV after soaking in water and to -26 mV in culture medium. In contrast, HAP had a zeta potential of -11 mV in a dry state, -9 mV in water and -29 mV in culture medium. Concentrations of calcium and phosphate dissolved in distilled water showed the solubility was higher for TCP than for HAP. In comparison, it was found that dissolved calcium and phosphate in the medium were removed from the solution by deposition on immersed TCP-HAP ceramics. These results suggested that the stability of the surface was closely related to both reactions of association and dissociation of calcium and phosphate in tissue culture medium. The zeta potential analysis also suggested that Ca-deficient HAP, which has a similar crystal structure to HAP with a Ca/P ratio less than 1.67, was generated by degradation and reforming of the surface layer. The most stable structure which was the most suitable for adhesion of L-929 cells was obtained by the mixture of 20% TCP and 80% HAP ceramics. In conclusion, the stability of the surface structure was considered to be the dominant factor for the enhancement of the adhesiveness of cells on TCP-HAP ceramics. (C) 1997 John Wiley & Sons, Inc.
  • Characteristics of silica-chitosan complex membrane and their relationships to the characteristics of growth and adhesiveness of L-929 cells cultured on the biomembrane, T Suzuki, Y Mizushima, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(2), 128 - 132, 1997 , Refereed
    Summary:The relationships between the chemical and physical characteristics of a silica-chitosan complex membrane (SiCM) and the characteristics of growth and adhesion of fibroblast-like L-929 cells cultured on the surface of SiCM were investigated for development of a novel implant biomaterial for use as artificial skin. Silicon ethoxide was used to cross-link the chitosan complex membrane to enhance its oxygen permeability. The composition of the SiCM was stepwise controlled by adjustment of the mixing ratio between silica and chitosan, for silica contents of 20, 33, 43, and 50 wt%. The zeta potential of the SiCM varied negatively according to the silica content, from +20 mV for chitosan membrane (0%-SiCM) to -0.6 mV for 43%-SiCM. Similarly, the wet contact angle decreased from 68 degrees to 41 degrees according to the increase in the silica content up to 43%. However, significant increases in the wet contact angle and the zeta potential occurred upon further increase of the silica content to 50%. The adhesion and growth characteristics of L-929 cells on SiCM were found to be related to the surface properties of the SiCMs. The cell proliferation rate was higher on the 0, 20 and 50%-SiCMs than on a typical polystyrene culture dish. The strongest cell adhesion was obtained on 50%-SiCM among the complex membranes. Thus, the surface wet contact angle and the zeta potential of 50%-SiCM, which is highly permeable to oxygen, were found to be suitable for L-929 cell adhesion and growth, and therefore to promote tissue culture on the SiCM in vitro.
  • A dense cell culture system for microorganisms using a shake flask incorporating a porous ceramic filter, T Suzuki, Y Kamoshita, R Ohashi, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(2), 133 - 137, 1997 , Refereed
    Summary:A novel reactor design incorporating a porous ceramic tubular membrane fitted inside a shake flask was developed for the dense cell culture of microorganisms on an ordinary shaker. The tubular membrane was effective in extracting the culture broth. The filtering performance was found to be enhanced by fitting the filter at the shoulder of the flask. Membrane fouling was prevented for long periods by waves created in the culture broth due to the shaking action, which constantly washed the membrane surface. Using this shake flask system, the concentration of Saccharomyces cerevisiae cell mass reached 235 g/l at 220 h with the feeding of fresh medium and extraction of the supernatant. The long-lasting and high permeability performance of the ceramic membrane enabled increases in the concentration and productivity of viable cells to be readily achieved by continuously refreshing the culture supernatant.
  • Effect of surface instability of calcium phosphate ceramics on growth and adhesion of osteoblast-like cells derived from neonatal rat calvaria, T Suzuki, M Hukkanen, LDK Buttery, JM Polak, Y Yokogawa, T Nishizawa, F Nagata, Y Kawamoto, T Kameyama, M Toriyama, BIOCERAMICS, VOL 10, BIOCERAMICS, VOL 10, 10, 105 - 108, 1997 , Refereed
    Summary:Using composite sinters of hydroxyapatite (HAP) and beta-tricalcium phosphate (TCP) as culture carriers, whose Ca/P molar ratios were adjusted in stepwise fashion to values of 1.50, 1.55, 1.60, 1.64 and 1.67, osteoblast-like cells derived from neonatal rat calvaria and a mouse fibroblast L-929 cell line were cultured and cell growth rates, adhesion and metabolism were investigated. Growth rates of both cells were accelerated on the TCP-HAP ceramics compared to zirconia ceramics. Adhesiveness of osteoblast-like cells on TCP-HAP ceramics was also enhanced more than on zirconia alone. Measurement of alkaline phosphatase activity showed that the cellular activity was enhanced by culturing osteoblast-like cells with high concentrations of calcium. These results suggest that the surface of TCP-HAP ceramics, especially 100% HAP ceramics, is effective to accelerate growth and differentiation of osteoblast-like cells.
  • Ethanol production in an immobilized cell reactor coupled with the recycling of effluent from the bottom of a distillation column, M Kishimoto, Y Nitta, Y Kamoshita, T Suzuki, K Suga, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(5), 449 - 454, 1997 , Refereed
    Summary:An ethanol fermentation system consisting of an immobilized cell reactor and a distillation column was constructed for recycling the effluent from the bottom of a distillation column. The effluent contained medium components and Ca ion, which were needed to stabilize the activity level of immobilized cells. We studied the durability of the ethanol production system in which only glucose aqueous solution was added and ethanol was harvested from the top of the distillation column without waste during the cultivation. We confirmed that the ethanol production could continue for 500 h, and that the experimental ethanol production yield was equivalent to 92% of the theoretical yield. It was assumed that the major portion of the glucose was consumed for ethanol production in this system, while the other components were used primarily for the growth and maintenance of the cells. The cost of the medium and the wastewater treatment for ethanol production by the proposed system is much lower than that of the conventional ethanol production process, because the amount of the medium components, except glucose, added during the process was much less than in the conventional alcohol production process. Furthermore, the amount of wastewater was around 6% of that produced in the conventional process. A preliminary automatic control sytem for the maintenance of the ethanol production was also constructed in order to avoid manual operation in nighttime. The glucose feed rate and the time interval of the distillation were kept constant at night. The computer controlled the on-off operation of the feeding pump in order to avoid excess feeding, which caused the liquid volumes in the bioreactor and the bottom vessel of distillation system to be above the critical volume levels. From the culture experiments, we confirmed that our control system would be effective for the reliable operation of an ethanol fermentation system and would eliminate manual operation in nighttime.
  • Characteristics of silica-chitosan complex membrane and their relationships to the characteristics of growth and adhesiveness of L-929 cells cultured on the biomembrane, T Suzuki, Y Mizushima, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(2), 128 - 132, 1997 , Refereed
    Summary:The relationships between the chemical and physical characteristics of a silica-chitosan complex membrane (SiCM) and the characteristics of growth and adhesion of fibroblast-like L-929 cells cultured on the surface of SiCM were investigated for development of a novel implant biomaterial for use as artificial skin. Silicon ethoxide was used to cross-link the chitosan complex membrane to enhance its oxygen permeability. The composition of the SiCM was stepwise controlled by adjustment of the mixing ratio between silica and chitosan, for silica contents of 20, 33, 43, and 50 wt%. The zeta potential of the SiCM varied negatively according to the silica content, from +20 mV for chitosan membrane (0%-SiCM) to -0.6 mV for 43%-SiCM. Similarly, the wet contact angle decreased from 68 degrees to 41 degrees according to the increase in the silica content up to 43%. However, significant increases in the wet contact angle and the zeta potential occurred upon further increase of the silica content to 50%. The adhesion and growth characteristics of L-929 cells on SiCM were found to be related to the surface properties of the SiCMs. The cell proliferation rate was higher on the 0, 20 and 50%-SiCMs than on a typical polystyrene culture dish. The strongest cell adhesion was obtained on 50%-SiCM among the complex membranes. Thus, the surface wet contact angle and the zeta potential of 50%-SiCM, which is highly permeable to oxygen, were found to be suitable for L-929 cell adhesion and growth, and therefore to promote tissue culture on the SiCM in vitro.
  • Regional changes in angiotensin II receptor density after experimental myocardial infarction, DC Lefroy, J Wharton, T Crake, GA Knock, RAD Rutherford, T Suzuki, K Morgan, JM Polak, PA PooleWilson, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 28(2), 429 - 440, Feb. 1996 , Refereed
    Summary:The plasma and cardiac renin-angiotensin systems may be activated after myocardial infarction. The myocardium may therefore be exposed to increased concentrations of angiotension II, which may contribute to myocardial injury. The purpose of this study was to identify the potential sites of action of angiotensin II in the infarcted heart. Myocardial infarction was induced in rats by left coronary artery ligation, and the hearts were removed for study after 18 h, 7 days, or 8 months. The regional ventricular angiotensin II receptor density was assessed by [I-125](Sar(1),Ile(8))angiotensin II binding and quantitative autoradiography. The [I-125](Sar(1),Ile(8))angiotensin II binding was unchanged at 18 h, but was increased at 7 days in the infarcted region of the left ventricle (73.2 +/- 3.2 amol/mm(2), mean +/- S.E.M.) compared with the non-infarcted region (1.6 +/- 0.2 amol/mm(2), P<0.0001) and with the left ventricular myocardium of sham-operated control animals (1.3 +/- 0.1 amol/mm(2), P<0.0001). The increased [I-125](Sar(1),Ile(8))angiotensin II binding density was still present, but diminished, at 8 months after coronary ligation (49.0 +/- 5.7 amol/mm(2), P<0.0001 nu control, P=0.0058 nu 7-day infarcts). The increased binding of [I-125](Sar(1),Ile(8))angiotensin II was antagonised by losartan, an AT(1) receptor antagonist, but not by an AT(2) receptor antagonist. Microautoradiography of [I-125](Sar(1),Ile(8)) angiotensin II, and assessment of collagen deposition using picrosirius staining and immunostaining demonstrated that the regional increase in AT(1) receptor density in the infarcted region of myocardium was associated with fibroblast infiltration and collagen deposition. The infarct scar and the cardiac fibroblasts within it express high levels of angiotension II receptors and therefore represent potential targets for the actions of angiotensin II after myocardial infarction. 1996 Academic Press Limited
  • Time-dependent variation of the surface structure of bioceramics in tissue culture medium and the effect on adhesiveness of cells, T Suzuki, K Nishizawa, Y Yokogawa, F Nagata, Y Kawamoto, T Kameyama, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 81(3), 226 - 232, 1996 , Refereed
    Summary:Biocompatible ceramics made of beta-tricalcium phosphate (TCP) were found to be actively changing their surface characteristics in tissue culture medium. The time-dependent variation of the zeta potential of TCP ceramics immersed in distilled water and in culture medium with and without addition of fetal bovine serum showed that the surface was unstable with significant changes in the charge being measured. Dry TCP had a zeta potential of -9.3 mV, which shifted to -1.8 mV after soaking in water and to -27.6 mV in culture medium with serum. To clarify the effect of the time-dependent variation of the surface structure on growth and adhesion of anchorage-dependent animal cells, the zeta potential of ceramics in dry state was regulated from -11.5 mV to + 27.2 mV by means of silane coupling modification. After soaking in distilled water for six days, zeta potential of the modified TCPs shifted to between +7.5 mV and -7.6 mV, while they were between -9.9 mV and -23.7 mV in culture medium with serum, Concentrations of calcium and phosphate dissolved in distilled water and in culture medium showed the solubility of the ceramics was considerably high and depended on the pH of the surface layer. The suitable surface property for adhesion of L-929 cells was obtained by the most stable ceramics in the culture condition. In conclusion, the solubility of the thin surface layer of the carrier was considered to be the dominant factor in decreasing the adhesiveness of cells on TCP ceramics.
  • Hydroxyapatite coating on alumina ceramics by an oxidative decomposition method of EDTA-calcium chelate, M Toriyama, Y Kawamoto, T Suzuki, Y Yokogawa, K Nishizawa, F Nagata, MR Mucalo, JOURNAL OF MATERIALS SCIENCE LETTERS, JOURNAL OF MATERIALS SCIENCE LETTERS, 15(2), 179 - 181, Jan. 1996 , Refereed
  • Preparation of calcium strontium apatite through mechanochemical method, Y Yokogawa, M Toriyama, Y Kawamoto, T Suzuki, K Nishizawa, F Nagata, MR Mucalo, CHEMISTRY LETTERS, CHEMISTRY LETTERS, 1996(1), 91 - 92, 1996 , Refereed
    Summary:The formation of a calcium-strontium apatite solid solution precursor via mechanochemical method was described. The precursor is formed by a process of ball-milling of a slurry consisting of a mixture of the starting materials; CaHPO4.2H(2)O, CaCO3, SrHPO4 and Sr(OH)(4).8H(2)O in a zirconia pot mill for 24 h in the presence of water at room temperature over the entire Ca/(Ca+Sr) compositional range. Calcium-strontium apatite precursor obtained from bail-milling began to change to calcium-strontium apatite upon heating at temperature over 500 degrees C.
  • Growth and adhesion of osteoblasts derived from neonatal rat calvaria on calcium phosphate ceramics, M Hukkanen, T Suzuki, T Yamamoto, LDK Buttery, FJ Hughes, JM Polak, JOURNAL OF PATHOLOGY, JOURNAL OF PATHOLOGY, 179, A4 - A4, 1996
  • A dense cell culture system for microorganisms using a stirred ceramic membrane reactor incorporating asymmetric porous ceramic filters, T Suzuki, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 82(3), 264 - 271, 1996 , Refereed
    Summary:A stirred ceramic membrane reactor (SCMR) incorporating asymmetric porous ceramic tubes was found to be effective for maintaining high filtrate flux over a long period of filtering culture. Alumina ceramic fillers with mean pore diameters of 0.5 mu m at the surface and 25 mu m in the inner rough phase were fitted inside a jar-fermentor, Filtering performance was enhanced by stirring the culture broth at a higher rate, Membrane fouling was also prevented by stirring, Using this bioreactor system, a dense cell culture of Lactococcus lactis, which is damaged by metabolites produced in the culture supernatant, was attempted by the filtering culture method with the aid of an automatic feeding and altering system. Preliminary tests on the medium composition in batch cultures showed that the cell growth was severely impaired by excessive consumption of substrates such as glucose, Polypepton, and yeast extract, Even in a fed-filtering culture, the cell concentration was as low as 38 g/l with 56% viability after a 220-h cultivation period with the feeding of a substrate solution containing 12 g/l glucose and 4 g/l yeast extract powder. A high cell concentration of 141 g/l with 94% viability after 238-h cultivation was achieved by fed-filtering massive amounts of a diluted substrate solution containing 6 g/l glucose and 2 g/l yeast extract. Both the yield and productivity of cells were also increased by controlling the feeding of the diluted fresh medium and filtering the supernatant of the dense cell culture. The filtrate volume reached sixty-four times that of the original working volume in the fermentor. The average dilution rate was 0.3 h(-1) through the filtering culture, These results showed that the long-lasting and high permeability capacity of the SCMR system fitted with an asymmetric membrane enabled facilitated increases in the concentration and productivity of the cells by continuously removing inhibitory by-products at a high dilution rate.
  • FURTHER-STUDIES OF CALCIUM-PHOSPHATE GROWTH ON PHOSPHORYLATED COTTON FIBERS, MR MUCALO, Y YOKOGAWA, T SUZUKI, Y KAWAMOTO, F NAGATA, K NISHIZAWA, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 6(11), 658 - 669, Nov. 1995 , Refereed
    Summary:Further studies using scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX), micro-fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and solid state magic angle spinning nuclear magnetic resonance (MAS NMR) techniques of calcium phosphate growth on Ca(OH)a-treated urea/H3PO3- and urea/H3P4-modified cotton fibres are reported. In the case of the Ca(OH)(2)-treated urea/H3PO3-modified fibres which have been reported in an earlier paper, further experiments subjecting the urea/H3PO3-modified cotton to alternative soaking treatment procedures to Ca(OH)(2) as well as different calcium phosphate growth media such as the alkaline phosphatase-catalysed hydrolysis of disodium p-nitrophenyl phosphate to free phosphate have reaffirmed the importance of the Ca(OH)(2) treatment step for the stimulus and growth of calcium phosphate growth on the fibres. Studies on cotton phosphorylated by a slightly different method using urea/H3PO4 instead of urea/H3PO3 show that a phosphorylated cotton with similar properties to the urea/H3PO3-modified fibres can be produced. Soaking of these fibres in saturated Ca(OH)(2) solution leads to cotton coated with thin layers of calcium phosphate formed by partial hydrolysis of the PO4 functionalities in the phosphorylated cotton which are believed to act as nucleation layers for further calcium phosphate deposition when the fibres are subsequently soaked in 1.5 x SBF solution. SEM/EDX studies of the calcium phosphate coatings formed on the Ca(OH)(2)-treated urea-H3PO4 fibres as a function of soaking time in 1.5 x SBF show that coatings deposit and become noticeably thick after approximately 9 days. XPS studies indicated the presence of carbonate species in the calcium phosphate coating deposited. In common with the calcium phosphate coated Ca(OH)(2)-treated urea/H3PO3-modified fibres studied earlier, the average EDX-measured Ca:P ratios of the coatings formed on the Ca(OH)(2)-treated urea/H3PO4 fibres are similar to 1.60 and give very similar micro-FTIR spectra with evidence of carbonate which suggests that amorphous calcium deficient apatite has deposited.
  • GROWTH OF CALCIUM-PHOSPHATE ON SURFACE-MODIFIED COTTON, MR MUCALO, Y YOKOGAWA, M TORIYAMA, T SUZUKI, Y KAWAMOTO, F NAGATA, K NISHIZAWA, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 6(10), 597 - 605, Oct. 1995 , Refereed
    Summary:A study of the growth of amorphous calcium phosphate on surface-modified cotton fibres by a combination of scanning electron microscopy/electron diffraction X-ray analysis, micro-FTIR and X-ray photoelectron spectroscopy is reported. Cotton fibres phosphorylated by the urea/phosphorous acid method and then soaked in saturated Ca(OH)(2) for approximately one week were found to stimulate the growth of a calcium phosphate coating on their surfaces after soaking in 1.5 x SBF for as little as 1 day. Ca(OH)(2) soaking of the fibres is found to produce highly crystalline clusters lodged in the fibres which were confirmed by micro-FTIR to be calcium phosphite monohydrate (CaHPO3 . H2O). In contrast, phosphorylated fibres not subjected to the Ca(OH)(2) treatment did not exhibit calcium phosphate growth upon immersion in 1.5 x SBF solution. Soaking of the Ca(OH)(2)-treated fibres with time in the 1.5 x SBF solution produced progressively thicker layers of calcium phosphate growth upon immersion in 1.5 x SBF solution. Soaking of the Ca(OH)(2)-treated fibres with time in the 1.5 x SBF solution produced progressively thicker layers of calcium phosphate on the fibres as confirmed by scanning electron microscopy and X-ray photoelectron spectroscopy. In general, calcium phosphate coatings formed over a 1-5 day period soaking in 1.5 x SBF solution appeared to consist of agglomerations of a large number of small spherical particles, while coatings formed after 17 days of soaking were distinctly chunky, thick and non-uniform in appearance. Micro-FTR indicated that CaHPO3 . H2O clusters were still present in cotton samples even after 4 days of soaking, while after 17 days, only the infrared spectrum typical of calcium phosphate was observed. EDX-measured Ca:P ratios of the coatings, although variable, suggested amorphous calcium phosphate. The mechanism of formation of the coating is believed to involve dissolution of the CaHPO3.H2O clusters upon introduction of the Ca(OH)(2)-treated phosphorylated cotton into the 1.5 x SBF solution which elevates the Ca2+ ion concentration in the vicinity of the fibres so stimulating calcium phosphate formation. It is postulated that phosphite groups chemically bound to the cotton fibre surface or a calcium phosphite coating on the fibres act as nucleation sites for calcium phosphate growth in 1.5 x SBF solution.
  • GROWTH OF CALCIUM-PHOSPHATE ON ION-EXCHANGE RESINS PRE-SATURATED WITH CALCIUM OR HYDROGENOPHOSPHATE IONS - AN SEM/EDX AND XPS STUDY, MR MUCALO, M TORIYAMA, Y YOKOGAWA, T SUZUKI, Y KAWAMOTO, F NAGATA, K NISHIZAWA, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 6(7), 409 - 419, Jul. 1995 , Refereed
    Summary:Calcium phosphate formed on the surfaces of ion-exchange resins pre-saturated with either Ca-2 + or HPO42- ions has been studied using a combination of scanning electron microscopy (SEM)/energy dispersive X-ray (EDX) analysis and X-ray photoelectron spectroscopy (XPS). Calcium phosphate was formed at a temperature of 36.5 degrees C via two methods. On Ca2+ or HPO42- -saturated resins, 1.5 x SBF (simulated body fluid) solution was used while on Ca2+ -saturated resins only, a novel biomimetic growth medium using the alkaline phosphatase-catalysed hydrolysis reaction of disodium p-nitrophenylphosphate as a source of inorganic phosphate was employed. SEM micrographs showed that the use of 1.5 x SBF growth medium solution led to extensive coverage of the resins with calcium phosphate. In contrast, calcium phosphate coatings formed via the alkaline phosphatase-catalysed reaction were of a more variable quality whose morphology could be influenced by adding albumin and collagen to the growth medium. Average Ca:P ratios determined by EDX for coatings formed from the 1.5 x SBF growth medium were in the range 1.62-1.74 suggesting that hydroxyapatite had formed. In contrast, Ca:P ratios for the calcium phosphate compounds formed on resins from the alkaline phosphatase reaction were lower at 1.50 suggesting that calcium-deficient hydroxyapatite had formed which was confirmed by inductively coupled plasma (ICP) analysis and X-ray diffraction of isolated amorphous and crystallized powder samples, respectively. Evidence from X-ray photoelectron studies supports a mechanism of formation of the coatings which involves diffusion of the ion out of the interior of the resin to create a high local concentration at the surface thus stimulating precipitation of the coating material on the resin beads.
  • AT(1) RECEPTOR CHARACTERISTICS OF ANGIOTENSIN ANALOG BINDING IN HUMAN SYNOVIUM, DA WALSH, T SUZUKI, GA KNOCK, DR BLAKE, JM POLAK, J WHARTON, BRITISH JOURNAL OF PHARMACOLOGY, BRITISH JOURNAL OF PHARMACOLOGY, 112(2), 435 - 442, Jun. 1994 , Refereed
    Summary:1 Angiotensin II (AII) reduces blood flow, modulates vascular remodelling and is a growth factor. Human inflammatory arthritides are characterized by synovial hypoperfusion, hypoxia and proliferation. We aimed to localize and characterize receptors for AII in human synovium. 2 We used quantitative in vitro receptor autoradiography with [I-125]-(Sar(1), Ile(8))AII and [I-125]-AII on human synovium from patients with chondromalacia patellae, osteoarthritis and rheumatoid arthritis. 3 [I-125]-(Sar(1), Ile(8))AII and [I-125]-AII bound to similar sites on synovial blood vessels, lining cells and stroma. Binding to microvessels (<100 mu m diameter) was more dense than to arteriolar media, and vascular binding was more dense than that to lining cells and stroma. 4 Microvessels and arterioles which displayed angiotensin converting enzyme-like immunoreactivity also displayed specific binding of [I-125]-(Sar(1), Ile(8))AII. 5 Specific binding of [I-125]-(Sar(1), Ile(8))AII to each structure was completely inhibited by 10 mu M dithiothreitol and was inhibited by unlabelled ligands with the rank order of potency (Sar(1), Ile(8))AII > AII > losartan = SKF108566 much greater than PD123319 indicating an AT(1) subclass of angiotensin receptor. 6 GTP gamma S (1 mu M) abolished specific binding of [I-125]-AII and abolished the high affinity component of the binding inhibition curve for AII against [I-125]-(Sar(1), Ile(8))AII, indicating G protein coupling. 7 The distribution of [I-125]-(Sar(1), Ile(8))AII binding sites was similar in all disease groups and no significant differences in binding densities, affinities or specificities were observed between disease groups. 8 Locally generated AII may act on synovial AT(1) receptors to modulate synovial perfusion and growth. Specific AT(1) receptor antagonists should help elucidate the role of angiotensins in human arthritis.
  • HYDROTHERMAL SYNTHESIS OF PLATE-LIKE HYDROXYAPATITE CRYSTALS, F NAGATA, Y YOKOGAWA, M TORIYAMA, Y KAWAMOTO, T SUZUKI, K NISHIZAWA, H NAGAE, ADVANCED MATERIALS '93, II - A & B, ADVANCED MATERIALS '93, II - A & B, 15(A & B), 11 - 14, 1994 , Refereed
  • A dense cell retention culture system using stirred ceramic membrane reactor, Takahiro Suzuki, Takeshi Sato, Minoru Kominami, Biotechnology and Bioengineering, Biotechnology and Bioengineering, 44(10), 1186 - 1192, 1994 , Refereed
    Summary:A novel reactor design incorporating porous ceramic tubes into a stirred jar fermentor was developed. The stirred ceramic membrane reactor has two ceramic tubular membrane units inside the vessel and maintains high filtration flux by alternating use for filtering and recovering from clogging. Each filter unit was linked for both extraction of culture broth and gas sparging. High permeability was maintained for long periods by applying the periodical control between filtering and air sparging during the stirred retention culture of Saccharomyces cerevisiae. The ceramic filter aeration system increased the kLa to about five times that of ordinary gas sparing. Using the automatic feeding and filtering system, cell mass concentration reached 207 g/L in a short time, while it was 64 g/L in a fed‐batch culture. More than 99% of the growing cells were retained in the fermentor by the filtering culture. Both yield and productivity of cells were also increased by controlling the feeding of fresh medium and filtering the supernatant of the dense cells culture. © 1994 John Wiley & Sons, Inc. Copyright © 1994 John Wiley & Sons, Inc.
  • EFFECTS OF THE SURFACE WETTABILITY AND ZETA-POTENTIAL OF BIOCERAMICS ON THE ADHESIVENESS OF ANCHORAGE-DEPENDENT ANIMAL-CELLS, K NISHIZAWA, M TORIYAMA, T SUZUKI, Y KAWAMOTO, Y YOKOGAWA, H NAGAE, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 75(6), 435 - 437, 1993 , Refereed
    Summary:Using calcium phosphate ceramics that have high biocompatibility with the living body, the effects of the surface characteristics of the bioceramics on cell adhesiveness were investigated. In the case of carriers with contact angles from 35-degrees to 60-degrees, the cell adhesiveness increased according to the increase in the wettability. Measurement of the zeta potentials of HAP-TCP sinters showed that these bioceramics had negative potentials from -2 mV to -6 mV. Electrochemical analysis suggested thal the initial cell anchoring ratio (R(ia)) and adhesive strength (F(a.enz)) were affected by the surface ionic condition of the ceramic material. To clarify the effects of the surface potential of the ceramics on cell adhesiveness, the ceramic surface was modified chemically by means of various silane coupling reagents. The surface potential was regulated from -20 mV to +24 mV. Using these ceramics, the affinity and adhesiveness of the cells to the ceramics were found to be dominantly regulated by the surface potential. A negative potential was effective in increasing the adhesiveness, even though living cells have negative charges.
  • THE ADHESIVENESS AND GROWTH OF ANCHORAGE-DEPENDENT ANIMAL-CELLS ON BIOCOMPATIBLE CERAMIC CULTURE CARRIERS, T SUZUKI, M TORIYAMA, Y KAWAMOTO, Y YOKOGAWA, S KAWAMURA, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 72(6), 450 - 456, 1991 , Refereed
    Summary:The growth of anchorage-dependent animal cells was significantly affected by the adhesiveness on carrier materials. The relationship between the cell division rate and adhesiveness was quantitatively studied by use of ceramic carriers whose chemical compositions were regulated stepwise. An apparatus using a spinner flask was designed to estimate numerically the adhesive strength of cells to ceramic carriers. Using this apparatus, the adhesive strength of cells to ceramic sinters made by mixtures of synthesized hydroxyapatite (HAP) and tricalcium phosphate (TCP), a polystyrene dish (LUX), a glass dish, and a ZrO2 ceramic plate were measured. The adhesiveness was comparably analyzed by trypsination method. Both measurements showed that the adhesiveness of L929 cells to calcium-phosphate sinters was considerably weaker than to LUX. Kinetic analyses of cell division and the adhesiveness to various carriers showed that a certain degree of weak adhesiveness was apparently advantageous for accelerating cell division. Another noteworthy and compatible correlation was obtained by the numerical analysis of growth phase dependent changes of these physiological parameters. The relationship between the cell division rate and adhesiveness based on the quantitative analysis was applicable for all the ceramic carriers used in this study. Measurement of the contact angle of the ceramic carrier with water suggested that the hydrophobicity of the carrier material was the dominant factor in determining the adhesiveness at the cell-carrier interface. The calcium-phosphate ceramic sinters had contact angle values from 42-degrees to 56-degrees. On the other hand, LUX, glass and ZrO2 carriers had more hydrophobic surfaces as the values were around 65-degrees. The most favored carrier for cell division had weak hydrophobicity with an angle of 51-degrees.
  • APPLICATION OF A MICROPOROUS GLASS-CERAMICS WITH A SKELETON OF CATI4(PO4)6 TO CARRIERS FOR IMMOBILIZATION OF ENZYMES, T SUZUKI, M TORIYAMA, H HOSONO, Y ABE, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 72(5), 384 - 391, 1991 , Refereed
    Summary:A porous ceramics developed by controlled crystallization of glass in the CaO-TiO2-P2O5 system (CTP) and subsequent acid leaching was found very durable in both acid and alkaline solutions because of the high TiO2 content. The characteristics of CTP as an immobilizing carrier for various enzymes were investigated. Invertase, beta-galactosidase, and alkalophilic proteinase were each immobilized to the porous ceramics, and the stabilities of the derivatives were observed in various pH conditions. The conventional inorganic carrier for enzymes, controlled pore glass (CPG), was used as a control test sample. The quantitative yield of beta-galactosidase initially attached to CTP was about four times smaller compared to CPG. The stabilities of CTP derivatives in solutions of pH 4.6 and pH 9 were remarkably enhanced, however. Even in a neutral pH solution, the retained hydrolytic activity of beta-galactosidase on CPG was rapidly lost at 37-degrees-C, while that of the CTP derivative was maintained for a long period. Since CPG is composed of 96% SiO2, its derivatives dissolve into various pH solutions more rapidly than CTP. Our results showed that the new microporous ceramics is a more suitable material than the conventional silica glass carriers to serve as an efficient and stable enzyme reactor for long term operations.
  • DEVELOPMENT OF A CULTURE CARRIER FOR ANCHORAGE-DEPENDENT ANIMAL-CELLS USING CALCIUM-PHOSPHATE CERAMIC SINTERS, T SUZUKI, M TORIYAMA, Y KAWAMOTO, Y YOKOGAWA, S KAWAMURA, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 70(3), 164 - 168, 1990 , Refereed
  • PHENOMENOLOGICAL BACKGROUND AND SOME PRELIMINARY TRIALS OF AUTOMATED SUBSTRATE SUPPLY IN PH-STAT MODAL FED-BATCH CULTURE USING A SETPOINT OF HIGH LIMIT, T SUZUKI, T YAMANE, S SHIMIZU, JOURNAL OF FERMENTATION AND BIOENGINEERING, JOURNAL OF FERMENTATION AND BIOENGINEERING, 69(5), 292 - 297, 1990 , Refereed
  • EFFECTIVE PRODUCTION OF PSEUDOMONAS-FLUORESCENS LIPASE BY SEMI-BATCH CULTURE WITH TURBIDITY-DEPENDENT AUTOMATIC FEEDING OF BOTH OLIVE OIL AND IRON-ION, K ISHIHARA, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 31(1), 45 - 48, Jul. 1989 , Refereed
  • EFFECT OF AMMONIUM FEEDING ON PRODUCTION OF THIOSTREPTON BY FED-BATCH CULTURE, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 28(2), 188 - 192, Apr. 1988 , Refereed
  • EFFECT AND CONTROL OF GLUCOSE FEEDING ON BACITRACIN PRODUCTION BY FED-BATCH CULTURE OF BACILLUS-LICHENIFORMIS, T SUZUKI, T YAMANE, S SHIMIZU, JOURNAL OF FERMENTATION TECHNOLOGY, JOURNAL OF FERMENTATION TECHNOLOGY, 66(1), 85 - 91, Feb. 1988 , Refereed
  • MASS-PRODUCTION OF LIPASE BY FED-BATCH CULTURE OF PSEUDOMONAS-FLUORESCENS, T SUZUKI, Y MUSHIGA, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 27(5-6), 417 - 422, Feb. 1988 , Refereed
  • MASS-PRODUCTION OF THIOSTREPTON BY FED-BATCH CULTURE OF STREPTOMYCES-LAURENTII WITH PH-STAT MODAL FEEDING OF MULTI-SUBSTRATE, T SUZUKI, T YAMANE, S SHIMIZU, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 25(6), 526 - 531, Mar. 1987 , Refereed
  • CONTROL OF CARBON SOURCE SUPPLY AND DISSOLVED-OXYGEN BY USE OF CARBON-DIOXIDE CONCENTRATION OF EXHAUST-GAS IN FED-BATCH CULTURE, T SUZUKI, T YAMANE, S SHIMIZU, JOURNAL OF FERMENTATION TECHNOLOGY, JOURNAL OF FERMENTATION TECHNOLOGY, 64(4), 317 - 326, 1986 , Refereed
  • DETECTION AND AUTOMATIC-CONTROL OF AMMONIUM ION CONCENTRATION IN MICROBIAL CULTURE WITH AN AMMONIUM ION-SELECTIVE ELECTRODE, T SUZUKI, T YASUDA, T YAMANE, S SHIMIZU, JOURNAL OF FERMENTATION TECHNOLOGY, JOURNAL OF FERMENTATION TECHNOLOGY, 64(1), 63 - 70, 1986 , Refereed
  • AUTOMATIC SUPPLEMENTATION OF MINERALS IN FED-BATCH CULTURE TO HIGH CELL MASS CONCENTRATION, T SUZUKI, H MORI, T YAMANE, S SHIMIZU, BIOTECHNOLOGY AND BIOENGINEERING, BIOTECHNOLOGY AND BIOENGINEERING, 27(2), 192 - 201, 1985 , Refereed
  • PROTEIN-PRODUCTION BY LYSIS WITH GLYCINE OF A FACULTATIVE METHYLOTROPH, PROTAMINOBACTER-RUBER, CULTIVATED IN A FED-BATCH SYSTEM, K SATO, K MARUYAMA, H MORI, T SUZUKI, S SHIMIZU, JOURNAL OF FERMENTATION TECHNOLOGY, JOURNAL OF FERMENTATION TECHNOLOGY, 62(3), 301 - 303, 1984 , Refereed
    Summary:A Protaminobacter ruber strain with a hard cell wall was cultivated to high cell concentration in a fed-batch system with antifoaming agent and lyzed by the addition of glycine in the logarithmic growth phase. By this procedure, a large amount of protein was accumulated in the medium.

Books etc

  • The cutting-edge mechanisms of skin aging and the latest development of effective materials for improvement or prevention of skin aging, Contributor,   2020 06 30 , 9784781315126
  • Nanotechnology for Producing Novel Cosmetics in Japan, Takahiro Suzuki, Contributor, Composite Powder, CMC,   2009 05 , 9784781301136

Conference Activities & Talks

  • Instrument evaluation method of physical properties related to feeling of use of cosmetics, Takahiro Suzuki, COSME Tech 2020 Tokyo,   2020 01 22
  • Powder tehcnology for make-up cosmetics, Takahiro Suzuki,   2019 10 25 , 招待有り
  • 肌水分率計の応答解析と餅面塗布試験法による化粧品の保湿効果の評価, 鈴木高広, 阪井博充, 坂本勝, SCCJ研究討論会講演要旨集,   2019 07 18
  • Analysis of the mechanism of huge earthquake occurrence due to crustal pumping motion caused by global warming and analysis of photosynthesis efficiency of sweetpotato by solar sharing farming method, 鈴木高広, 坂本勝, 太陽/風力エネルギー講演論文集,   2018 11 07
  • Current status and issues of development of potato energy aiming to realize RE 100 society, 鈴木高広, 坂本勝, 太陽/風力エネルギー講演論文集,   2018 11 07
  • 8th Seminar for the laest technology and new material R & D trend of makeup cosmetics, Takahiro Suzuki,   2018 06 08 , 招待有り, COSIN CO. LTD., Seoul, Korea
  • 4P-I132 Cheap way to produce sweetpotatoes to supply all calorie-consumption of the whole nation, at the 420,000ha-abandoned farmlands only, Takahiro Suzuki, Masaru Sakamoto, Wataru Morioka,   2017 09 14
  • 3-9-1 Developments of mass production method of sweetpotatoes and methane fermentation, SUZUKI Takahiro, SAKAMOTO Masaru, Proceedings of the Annual Conference of The Japan Institute of Energy,   2017 08 02
    Summary:

    Mass production of sweetpotatoes by a cheap cultivation method was achieved with small pots by optimizing the elongation space of the roots and the hygroscopic condition of the soil. In Okinawa we planted vine of sweetpotato in October, and harvested in April. The amount of sweetpotatoes cultivated in the winter months was reached to 7.3 kg/m2, which was three times the national average to grow in the summer. The result showed that annual production of 20kg/m2 sweetpotatoes is feasible by further optimizing the growth condition. For the purpose to use sweetpotatoes as fuel, technological issues of the methane fermentation and the gas power generation system were investigated.

  • Development of the semi-hydroponic system for mass production technologies of the sweetpotato, 鈴木 高広, 坂本 勝, 森岡 航, 石橋 俊介, 太陽/風力エネルギー講演論文集 = Proceedings of JSES/JWEA Joint Conference,   2016 11 24
  • Effect of root zone irrigation system on mass production of sweetpotatoes, SUZUKI Takahiro, SAKAMOTO Masaru, Proceedings of the Annual Conference of The Japan Institute of Energy,   2016 08 10
    Summary:

    The innovation is required to production of a large amount of biomass by cultivation of domestic photosynthetic plant and to replace fossil fuels. The author has found that the best crop is sweetpotato cultivated in multilayer system. So far, the 4-layers cultivation method using a triangular shelf showed that it is possible to sweetpotato production of about 20kg/m2. This is eight times the amount of domestic average yield (2.5kg/m2). However, the soil hygroscopic level decreased quickly and the production of the sweetpotatoes suppressed significantly, were found by the multi-layer cultivation system. Therefore we investigated the influence of the basic irrigation state to produce sweetpotatoes. As the result the yield of sweetpotatoes cultivated with root zone irrigation system increased to 179% of the amount of the past. In addition, the root zone irrigation system was effective to increase the weight of the mass production of sweetpotatoes by increasing the density of the vine seeding per area.

  • A practical application of dispersion use of sunlight in planter cultivation method for agricultural reconstruction assistance in Fukushima, Takahiro Suzuki, Akira Horibatam, Masaru Sakamoto, Michio Furukawa, Osamu Kanda, 太陽/風力エネルギー講演論文集,   2015 11 27
  • 太陽光分散型サツマイモ多層栽培システムにおける段階的収穫操作の最適化, SUZUKI TAKAHIRO, SAKAMOTO MASARU, 日本生物環境工学会大会講演要旨,   2015 09 09
  • 3-8-4 Optimization of sunshine rates in each layer of the multilayer cultivation system of sweetpotato, SUZUKI Takahiro, SAKAMOTO Masaru, Proceedings of the Annual Conference of The Japan Institute of Energy,   2015 08 03
    Summary:Innovation is required to alternative production of fossil fuels by domestic photosynthetic organisms. The author has found that the best crop is sweetpotato cultivated in multilayer system. So far, the 4-layers cultivation method using a triangular shelf showed that it is possible to sweetpotato production of about 20kg/m^2. This is eight times the amount of domestic average yield (2.5kg/m^2). To commercialize this cultivation method, the sunshine conditions with the development of low-cost production system was studied. As a result, a three-layers cultivation method which produced sweetpotatoes of 20kg/m^2 (about 150MJ/m^2 in the cultivation period 8 months, the conversion rate of about 3.5% of the sunlight) that can be realized has been found. These results showed that production of 15 million TJ renewable energy every year is possible, if cultivated sweetpotatoes at 10 million ha, 26% of the national land. Thus, the alternative production of total fossil fuels by domestic crop is feasible.
  • Mass production of the sweetpotato with a multilayered cultivation system, Takahiro Suzuki, 6th Japan-China-Korea Sweetpotato workshop,   2014 11 30 , 農研機構
  • 燃料用サツマイモの大量栽培技術の開発, SUZUKI TAKAHIRO, 太陽/風力エネルギー講演論文集,   2014 11 13
  • 1P-214 Growth properties of sweet potato in the triangle shelf multi-layer cultivation method and its methane fermentation condition, Suzuki Takahiro, Sakamoto Masaru, Ano Takashi, Shintani Noboru, 日本生物工学会大会講演要旨集,   2013 08
  • 三角棚を用いたサツマイモの日照多層栽培条件の検討, SUZUKI TAKAHIRO, SAKAMOTO MASARU, 日本生物環境工学会大会講演要旨集,   2013
  • 芋の遊休地多層連続大量生産による国内全エネルギー代替構想, SUZUKI TAKAHIRO, 日本生物環境工学会大会講演要旨,   2011 09
  • 2Ba01 Feasible and economical mass production system for fuel crops to replace fossil fuels of Japan, Suzuki Takahiro, 日本生物工学会第63回大会講演要旨集,   2011 08
  • 化粧品の顔料粉体とナノ粒子の肌面分散特性と使用感の解析, SUZUKI TAKAHIRO, UMEDA TOMOHIRO, 粉体工学会研究発表会講演論文集,   2010 11
  • 2P-2067 Management model and designing of plant bioreactors systems for high performance of quality and safety of agricultural products, Suzuki Takahiro, Horibata Akira, Umeda Tomohiro, Itao Kiyoshi, 日本生物工学会平成22年度大会講演要旨集,   2010 09
  • 作物生育特性と市場性に基づく植物工場収益性の予測と省エネ型光源を用いたプロセス制御, SUZUKI TAKAHIRO, HORIBATA AKIRA, UMEDA TOMOHIRO, ITAO KIYOSHI, 日本生物環境工学会大会講演要旨,   2010 09
  • 原料微粒子の表面設計による化粧品機能の評価と制御, NOMURA MUTSUKO, IIJIMA TOSHIYUKI, KAMIYA HIDEHIRO, IKKAI FUMIO, SUZUKI TAKAHIRO, 粉体工学会研究発表会講演論文集,   2008 10
  • シリカ/マイカ表面間付着力に及ぼすナノ粒子付着及び表面処理の影響, KAMITANI HIDEHIRO, TAMENGI MAKI, TSUKADA MAYUMI, KAJI TOSHIYUKI, SUZUKI TAKAHIRO, 化学工学会秋季大会研究発表講演要旨集(CD-ROM),   2007 08
  • シリカ微粒子/マイカ間付着力に及ぼす分子及びナノレベル表面構造制御の影響, TAMONOGI MAKI, TSUKADA MAYUMI, KAMIYA HIDEHIRO, KANI TOSHIYUKI, SUZUKI TAKAHIRO, 粉体工学会研究発表会講演論文集,   2005
  • Continuous production of lactic acid from molasses., Kasahara Norihide, Ueno Shigeaki, Ohashi Ryo, Suzuki Takahiro, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • High concentration production of PHB from waste water of lactic acid fermentation, Morimoto Toshiaki, Kasahara Norihide, Ohashi Ryo, Nagashima Hideyuki, Tashiro Fumio, Suzuki Takahiro, Yokogawa Yoshiyuki, Kameyama Tetsuya, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • Production of recombinant antibody fragment by E. coli using perfusion culture system., Ohashi Ryo, Chiba Joe, Suzuki Takahiro, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • Development ofa rapid and comtinuous fermentation system for production of lactic acid by improving the perfusion culture system., Ohashi Ryo, Kasahara Norihide, Sakaniwa Yukio, Suzuki Takahiro, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • Surface analyses of surface modified calcium phosphate ceramics during culturing anchorage- dependent animal cells., Nishizawa Kaori, Suzuki Takahiro, Yokogawa Yoshiyuki, Toriyama Motohiro, Nagata Fukue, Hozumi Atsushi, Kameyama Tetsuya, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • Continuous production of hydrogen by photosynthetic bacterium using perfusion culture system., Gunji Wataru, Ohashi Ryou, Suzuki Takahiro, 日本生物工学会平成11年度大会講演要旨集,   1999 08
  • 5-7. Continuous hydrogen production by perfusion culture of Clostridium paraputrificum using a stirred ceramic membrane reactor, Hashimoto Junichi, Ogawa Akinori, Ohashi Ryo, Evvyernie Dwierra, Suzuki Takahiro, Omiya Kunio, 日本エネルギー学会大会講演要旨集,   1999 07
  • 光触媒抗菌製品の抗菌力試験法について, SUZUKI TAKAHIRO, YAMADA ZEN'ICHI, MAEKAWA MASAAKI, YAMAGUCHI TAICHI, NAGAI TAKASHI, TAKAYAMA MASAHIKO, 日本防菌防ばい学会年次大会要旨集,   1999 05
  • The effects which a surface characterization of biomaterials has on NOS., UMEDA TOMOHIRO, OHASHI RYO, SUZUKI TAKAHIRO, TSUCHIYA TOSHIO, JERAMMY B, HUKKANEN M, POLAK J M, UMEDA T, 整形外科セラミック・インプラント研究会プログラム・抄録集,   1998 12
  • Porous Materials as Carriers for Immobilizing a Microorganism Prepared by Using Volcanic Ash and Oyster Shell., YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, KAWAMOTO YUKARI, NAGAE HAJIME, KAMEYAMA TETSUYA, 日本セラミックス協会秋季シンポジウム講演予稿集,   1998 10
  • Preparation of chitosan-silica, -Hap composites and biocompatibility in tissue culture test., Umeda Tomohiro, Ohashi Ryo, Suzuki Takahiro, Tsuchiya Toshio, 日本生物工学会平成10年度大会講演要旨集,   1998 08
  • Continuous and rapid production of hydrogen by perfusion culture of Cl. paraputrificum using a SCM flask., Suzuki Takahiro, Ohashi Ryo, Evvyernie Dwierra, Ohmiya Kunio, 日本生物工学会平成10年度大会要旨集,   1998 08
  • High-density culture of insect cell by perfusion culture using a SCM-reactor., Ohashi Ryo, Suzuki Takahiro, 日本生物工学会平成10年度大会講演要旨集,   1998 08
  • Cell lysis during the initial anchoring on calciumphoshate bone material., Suzuki Takahiro, Nishizawa Kaori, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Recycled-ceramics for water purification prepared by using volcanic ash and oyster-shell., Yokogawa Y, Nagae H, Suzuki T, Toriyama M, Kawamoto Y, Nishizawa K, Nagata F, Kameyama T, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Rapid and dense screening technique of PHA producing microorganisms using a SCM-flask, mass production by Paracoccus sp., and its application for biomaterial., Morimoto Toshiaki, Ohashi Ryo, Kamoshita Yuya, Kameyama Tetsuya, Kawamoto Yukari, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Improved antimicrobial activity test for photocatalytic reaction., Yamamoto Tomonori, Nakano Toru, Ohashi Ryo, Kamoshita Yuya, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • High concentration cell culture of Escherichia coli using a SCM flask with ventilation system., Kamoshita Yuya, Ohashi Ryo, Kamoshita Miwa, Kuragane Keizo, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • High concentration and High-Level Expression of Recombinant Escherichia coli using a SCM Flask., Kamoshita Yuya, Ohashi Ryo, Soga Takatoshi, Kamoshita Miwa, Chiba Joe, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Continuous production of lactic acid using a stirrerd ceramic membrane flask., Takano Masako, Naitou Yume, Ohasi Ryo, Kamoshita Yuya, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Continuous production of lactic acid using a stirrerd ceramic membrane reactor., Naito Yume, Takano Masako, Ohasi Ryo, Kamoshita Yuya, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • High-Level Expression of Recombinant Protein by Methanol-Inducible Transformed Pichia pastoris Using a SCM Flask System., Ohashi Ryo, Kamoshita Yuya, Mochizuki Eiko, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • High-Level Expression of Recombinant Protein by Methanol-Inducible Transformed Pichia paastoris Using a SCM Reactor System., Ohashi Ryo, Kamoshita Yuya, Mochizuki Eiko, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • 262 Induction of osteogenesis by osteoblast-like cells using a porous hydroxyapatite mateix : Effect of external stimulation, Espinosa F. F, Okada K, Kawamoto Y, Nishizawa K, Kameyama T, Suzuki T, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Effects of apatite matrix on physiological activities of rat-primary hepatpcyte., Naka Rumi, Ohashi Ryo, Okada Kohji, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Effects of surface structure of porous ceramics on osteogenesis by osteoblast-like cells in tissue culture medium., Nakano Tohru, Ohashi Ryo, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Characterization of silica-chitosan complex membrane as skin in tissue culture test., Kuragane Keizo, Ohashi Ryo, Mizushima Yasuyuki, Suzuki Takahiro, 日本生物工学会平成9年度大会講演要旨集,   1997 09
  • Preparation of lactic-acid/apatite composites and the biocompatibility in tissue culture test., Umeda Tomohiro, Ohasi Ryo, Suzuki Takahiro, Tuchiya Toshio, 日本生物工学会平成9年度大会要旨集,   1997 09
  • Growth and adhesion of osteoblast-like cells derived from neonatal rat calvaria on calcium phosphatase ceramics, M. Hukkanen, T. Suzuki, T. Yamamoto, L.D.K. Buttery, F.J. Hughes, J.M. Polak, Proceedings of the 43rd Annual Meeting of the Orthopaedic Research Society, San Francisco, California,   1997 02
  • Development of high-density bacteria body filtering culture flask integrated with ceramic film., MURAKAMI YOSHIHISA, KAMOSHITA YUYA, OHASHI RYO, SUZUKI TAKAHIRO, 日本農芸化学会関東支部受賞記念講演およびシンポジウム講演要旨集,   1996 11
  • Calcium Phosphate Formation on Phosphorylated Chitin Fibers., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, KAMEYAMA TETSUYA, REYES J, 日本セラミックス協会秋季シンポジウム講演予稿集,   1996 10
  • Surface Modification of Bioceramics by Chemical Coupling Reaction., NISHIZAWA KAORI, YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NAGATA FUKUE, KAWAMOTO YUKARI, TORIYAMA MOTOHIRO, KAMEYAMA TETSUYA, 日本セラミックス協会秋季シンポジウム講演予稿集,   1996 10
  • Porous Materials as Carriers for Immobilizing a Microorganism Prepared by Using Volcanic Ash from Fugen Mountain in Nagasaki Prefecture., YOKOGAWA YOSHIYUKI, SUZUKI TAKAHIRO, NAGAE HAJIME, KAWAMOTO YUKARI, NISHIZAWA KAORI, NAGATA FUKUE, TORIYAMA MOTOHIRO, KAMEYAMA TETSUYA, 日本セラミックス協会秋季シンポジウム講演予稿集,   1996 10
  • Waste water treatment using porous sinters composed of waste ceramics and sludge., Suzuki Takahiro, Toriyama M, Yokogawa Y, Kawamoto Y, Nagata F, Nishizawa K, Kameyama T, Nagae H, Kato K, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • Effect of surface structure of TCP-HAP composite ceramics on growth of osteoblast-like cells., Suzuki Takahiro, Ito Harumi, Yokogawa Y, Kawamoto Y, Nishizawa K, Nagata F, Kameyama T, Toriyama M, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • Molecular designing of bioceramics by chemical surface modification, Nishizawa Kaori, Toriyama Motohiro, Yokogawa Yoshiyuki, Nagata Fukue, Kawamoto Yukari, Kameyama Tetsuya, Suzuki Takahiro, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • Improvement of FDH production by high concentration culture of Paraccocus sp., Kamoshita Yuya, Satoh Tamao, Ohkubo Atuko, Ohashi Ryo, Taguchi Hayao, Suzuki Takahiro, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • Continuous Ethanol Production by Retained Cells Using a SCM-Reactor Connected with a Repeated Broth-Recycle System, Kamoshita Yuya, Ohashi Ryo, Suzuki Takahiro, Kishimoto Michimasa, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • The challenge for development of ultra-high density microbial cell culture system., Suzuki Takahiro, Kamoshita Yuya, Ohashi Ryo, 日本生物工学会平成8年度大会講演要旨集,   1996 09
  • Phosphorylation as a mthod for the induction of calcium phosphate growth, 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西澤かおり, 永田夫久江, 亀山哲也, Michael Roger Mucalo, Jocelyn Paz Reye, Second International Symposium on Inorganic Phosphate Materials '96, Nagoya,   1996 09
  • Growth and Adhesion of Osteoblasts Derived from Neonatal Rat Calvaria on Calcium Phosphate Ceramics, M Hukkanen, T Suzuki, T Yamamoto, LDK Buttery, FJ Hughes, JM Polak, Summer Meeting of Pathological Society of Great Britain and Ireland,   1996 07
  • Nitric oxide synthesa and cycloogygenase-2 in pseudosynovial membrane of loosened total hip prostheses, MVJ Hukkanen, J.Batten, ID McCarthy, T Suzuki, AK Aune, SPF Hughes, JM Polak, Sixth Workshop on Cells and Cytokines in Bone and Cartilage,   1996 06
  • Porous Materials as Carriers for Immobilizing a Microorganism Prepared by Using Volcanic Ash from Fugen Mountain in Nagasaki Prefecture., YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGATA FUKUE, NAGAE HAJIME, 日本セラミックス協会年会講演予稿集,   1996 04
  • A Preliminary Study of Hydroxyapatite Formation Mechanism via Hydrothermal Processing., NAGATA FUKUE, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, KAMEYAMA TETSUYA, 日本セラミックス協会年会講演予稿集,   1996 04
  • Nitric oxide synthesa and cycloogygenase-2 in pseudosynovial membrane of loosened total hip prostheses and osteoarthritic synovium, MVJ Hukkanen, J.Batten, ID McCarthy, T Suzuki, AK Aune, SPF Hughes, JM Polak, 172nd British Pathological Society,   1996 01
  • Stimulation of hydroxyapatite growth on cotton fibres by phosphorylation of cellulose, Y. YOKOGAWA, M.R. MUCALO, M. TORIYAMA, Y. KAWAMOTO, T. SUZUKI, K. NISHIZAWA, F. NAGATA, Proceedings of International Workshop on Advanced Ceramics '96,   1996
  • Development of biological water treatment system using recycled ceramic materials., Muraoka Yukiko, Suzuki Takahiro, 日本生物工学会平成7年度大会講演要旨集,   1995 09
  • Formation of calcium phosphate on surface modified polymer., YOKOGAWA YOSHIYUKI, NAGATA FUKUE, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, 日本セラミックス協会年会講演予稿集,   1995 03
  • Wettability of Calcium Phosphate Ceramics by Water., TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, NISHIZAWA KAORI, NAGATA FUKUE, 日本セラミックス協会年会講演予稿集,   1995 03
  • Growth of Calcium Phosphate on Surface Modified Cotton., MUCALO M R, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, NISHIZAWA KAORI, NAGATA FUKUE, SUZUKI TAKAHIRO, 日本化学会講演予稿集,   1995 03
  • Studies on Development of Biological Purification Systems Using Non-Reused Local Materials for the Improvement of Water Environment., INAMORI YUHEI, TAKAGI HIROO, MIZUOCHI MOTOYUKI, NISHIMURA OSAMU, SUZUKI TAKAHIRO, YOKOKAWA YOSHIYUKI, KAWAMOTO YUKARI, NISHIZAWA KAORI, TORIYAMA MOTOHIRO, 都市における環境保全計画手法の開発に関する総合研究 平成6年度 騒音・振動の防止及び評価に関する総合研究 地域密着型環境研究,   1995
  • Morphology of Hydroxyapatite Crystals Synthesized in the Presence of Organic Solution., NAGATA FUKUE, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, 日本セラミックス協会秋季シンポジウム講演予稿集,   1994 10
  • Estimation and rescue control of physiological crisis of cells in pH-stat culture rising a SCM reactor., Suzuki Takahiro, Kawamoto Yukari, Nishizawa Kaori, Sato Takeshi, Ohmiya Kunio, 日本生物工学会平成6年度大会講演要旨集,   1994 10
  • Continuous treatment of waste water using immobilized Effective Microorganisms (EM) on porous ceramics., Nagatomi Hisashi, Suzuki Takahiro, Nagae Hajime, Kawamoto Yukari, Nishizawa Kaori, Kato Katsuya, Nagata Fukue, Toriyama Motohiro, 日本生物工学会平成6年度大会講演要旨集,   1994 10
  • Preparation and biocompatibility of chitosan-silica complex membrane., MIZUSHIMA YASUYUKI, SUZUKI TAKAHIRO, TORIYAMA MOTOHIRO, 日本セラミックス協会年会講演予稿集,   1994 04
  • Effect of CO32- Content on the Shape of Hydroxyapatite., NAGATA FUKUE, YOKOGAWA YOSHIYUKI, SANAMI TAKESHI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NOGAMI MASAYUKI, 日本セラミックス協会年会講演予稿集,   1994 04
  • Control of stirred ceramics membrane reactor by multistat method., Sato Takeshi, Kadotani Yasuyuki, Suzuki Takahiro, 日本生物工学会平成5年度大会講演要旨集,   1993 11
  • アルコール共存下における水酸アパタイト微結晶の水熱合成, 永田夫久江, 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 長江肇, 日本化学会講演予稿集,   1993 09
  • バイオセラミックスの表面改質と細胞の付着に対する担体表面の濡れ性とゼータ電位の影響, 西沢かおり, 鳥山素弘, 鈴木高広, 河本ゆかり, 横川善之, 永田夫久江, 長江肇, 日本化学会講演予稿集,   1993 09
  • Hydrothemal synthesis of ultrafine plate-like hydroxyapatite crystals in the presence of organic solvent., 永田夫久江, 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 長江肇, 日本セラミックス協会年会講演予稿集,   1993 03
  • Apatite coating on saphire substrate., 横川善之, 森下史康, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 永田夫久江, 長江肇, 野上正行, 日本セラミックス協会年会講演予稿集,   1993 03
  • Hydroxyapatite formation from solution on polymer., 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 永田夫久江, 長江肇, 日本セラミックス協会年会講演予稿集,   1993 03
  • Synthesis of hydroxyapatite in hydrothermal alcoholic solutions., 永田夫久江, 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 長江肇, セラミックス基礎科学討論会講演要旨集,   1993 01
  • Characterization and biocompatibility of calcium phosphate ceramics in serum medium for tissue culture., Yamamoto Takeyuki, Suzuki Takahiro, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Application of porous glass-ceramics to column chromatography of proteins., Imai K, Yumioka Y, Hosono H, Abe Y, Suzuki T, Toriyama M, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Surface modification of bioceramics and effects of surface hydrophobicity and surface potential for cell attachment and growth., Nishizawa K, Toriyama M, Suzuki T, Kawamoto Y, Yokogawa Y, Nagae H, Kamaguchi F, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Application of porous inorganic materials to continuous recycling culture supernatant of animal cells., Sakakibara N, Imai K, Hosono H, Abe Y, Suzuki T, Toriyama M, Nishizawa K, Yokogawa Y, Nagae H, Kawamoto Y, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Synthesis of porous ceramic carrier for immobilization of enzyme., Toriyama Motohiro, Kawamoto Yukari, Yokogawa Yoshiyuki, Nagae Hajime, Nishizawa Kaori, Suzuki Takahiro, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Control of fed-batch culture of anaerobe for chitinase production., Sugimoto Naomi, Karita Shuichi, Sakka Kazuo, Ohmiya Kunio, Shimada Kyo, Suzuki Takahiro, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Development and control of stirred ceramic membrane reactor system., Sato Takeshi, Suzuki Takahiro, 日本生物工学会平成4年度大会講演要旨集,   1992 10
  • Preparation of Porous Apatite Ceramics., YOKOGAWA YOSHIYUKI, MURAKAMI HIRONOBU, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, NAGAE HAJIME, NOGAMI MASAYUKI, 日本セラミックス協会年会講演予稿集,   1992 05
  • キレート酸化分解法による水酸アパタイトの合成, 鳥山素弘, 河本ゆかり, 鈴木高広, 横川善之, 西沢かおり, 長江肇, 日本化学会講演予稿集,   1992 03
  • Preparation of Sr-orthophosphate by Mechanochemical Reaction., 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 長江肇, セラミックス基礎科学討論会講演要旨集,   1992 01
  • Synthesis of tetracalcium phosphate., 横川善之, 鳥山素弘, 河本ゆかり, 鈴木高広, 西沢かおり, 長江肇, 無機リン化学討論会講演予稿集,   1992
  • Synthesis of Calcium Phosphate Compounds by an Oxidative Decomposition Method of Calcium Chelate., 鳥山素弘, 河本ゆかり, 鈴木高広, 横川善之, 西沢かおり, 長江肇, 無機リン化学討論会講演予稿集,   1992
  • Effects of ζ-ion potential and surface polarity of bioceramic materials for cell adhesion and growth., Nishizawa K, Suzuki T, Kawamoto Y, Toriyama M, Yokogawa Y, Nagae H, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Adsorption of serum protein to bioceramics, and its effects on cell growth., Kawamoto Y, Nishizawa K, Suzuki T, Toriyama M, Yokogawa Y, Nagae H, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Bioaffinity test on ceramic materials in application for animal cell-reactor., Suzuki T, Toriyama M, Kawamoto Y, Nishizawa K, Yokogawa Y, Nagae H, Tsuzuki M, Kondo K, Matsuo Y, Irie H, Hakamatsuka Y, Yamamoto T, Mizuno H, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Bioindustrial application of waste bread, Sato T, Kominami M, Shinke R, Konohana T, Suzuki T, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Study on forcast and control for fermentation process., Suzuki Takahiro, Prior John, O'Connor Gregory, Cooney Charles L, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Development of Stirred Ceramic Membrane Reactor (SCMR) for bioprocesses., Suzuki T, Sato T, 日本醗酵工学会平成3年度大会講演要旨集,   1991 10
  • Phase Relation in the .BETA.-TCP-Al2O3-SiO2 System., YOKOGAWA YOSHIYUKI, KOJIMA IKUNORI, KAWAMOTO YUKARI, TORIYAMA MOTOHIRO, SUZUKI TAKAHIRO, KAWAMURA SUKEZO, NOGAMI MASAYUKI, 日本セラミックス協会年会講演予稿集,   1991 05
  • .BETA.-TCP Coating on Alumina Ceramics., KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMURA SUKEZO, SUZUKI TAKAHIRO, 日本セラミックス協会年会講演予稿集,   1991 05
  • Characterization of CaTi_4(PO_4)_6 Porous Glass-Ceramics as Carrier for Immobilization of Enzymes., Maenami H, Hosono H, Abe Y, Suzuki T, Toriyama M, 日本醗酵工学会平成3年度大会講演要旨集,   1991
  • Characterization and improvement of biocompatible ceramic carrier for production of plasminogen activator by transformed human kidney cell., Yamamoto Takeyuki, Suzuki T, 日本醗酵工学会平成3年度大会講演要旨集,   1991
  • Development of a Porous Crystal Glass Carrier for Immobilization of Enzymes., Naruse T, Maenami H, Hosono H, Abe Y, Suzuki T, Toriyama M, Suzuki H, 日本醗酵工学会平成2年度大会講演要旨集,   1990 10
  • Study on the adhesion and growth of cells on the ceramic carriers., Suzuki T, Toriyama M, Kawamura S, Kawamoto Y, Yokogawa Y, 日本醗酵工学会平成2年度大会講演要旨集,   1990 10
  • バイオアクティブセラミックス担体上の付着依存性細胞培養特性, 鈴木高広, 鳥山素弘, 川村資三, 河本ゆかり, 横川善之, 化学工学会秋季大会研究発表講演要旨集,   1990 09
  • Synthesis of trimagnesium phosphate by mechanochemical reaction., 横川善之, 河本ゆかり, 鳥山素弘, 鈴木高広, 川村資三, 人工鉱物討論会講演要旨集,   1990 09
  • Where shall bionics research go?, SORA TATSUO, MIYAKE ATSUSHI, NISHIHARA KAZUE, YAMANE SHIGERU, FUKUI IKUO, SAKAGUCHI TAKAYUKI, SUZUKI TAKAHIRO, HAYASHI KAZUKO, UKEGAWA KOJI, 生体材料,   1990 06
  • Behavior of yttrium ion in Y-PSZ coated with .BETA.-TCP., KAWAMOTO YUKARI, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMURA SUKEZO, SUZUKI TAKAHIRO, 日本セラミックス協会年会講演予稿集,   1990 05
  • .BETA.-TCP coating on Y-PSZ by using calcium metaphosphate and tetracalcium phosphate., YOKOGAWA YOSHIYUKI, KAWAMOTO YUKARI, TORIYAMA MOTOHIRO, KAWAMURA SUKEZO, SUZUKI TAKAHIRO, 日本セラミックス協会年会講演予稿集,   1990 05
  • Development of tissus culture ceramics carrier using calcium phosphate materials., SUZUKI TAKAHIRO, TORIYAMA MOTOHIRO, KAWAMURA SUKEZO, KAWAMOTO YUKARI, YOKOKAWA YOSHIYUKI, 化学工学会年会研究発表講演要旨集,   1990 03
  • Forecast and expert control for fermentation process., Suzuki Takahiro, O'Connor, Gregory M, Cooney Charles L, 日本醗酵工学会平成元年度大会講演要旨集,   1989 10
  • Effective Lipase Production by Turbidity-dependent Automatic Fed-batch Culture., Ishihara Kazuto, Suzuki Takahiro, Yamane Tsuneo, Shimizu Shoichi, 日本醗酵工学会昭和63年度大会講演要旨集,   1988 11
  • Compound substrate fed-batch cultivation according to pH-stat method., SUZUKI TAKAHIRO, YAMANE TSUNEO, SHIMIZU SHOICHI, 化学工学協会年会研究発表講演要旨集,   1988 03
  • 流加培養法によるリパーゼ生産の効率化(生物化学工学-固定化微生物, 培養工学-), 石原 一人, 山根 恒夫, 鈴木 高広, 清水 祥一, 日本農藝化學會誌,   1988 03
  • 流加培養法による抗生物質バシトラシン生産の効率化(II)(生物化学工学-固定化微生物, 培養工学-), 鈴木 高広, 山根 恒夫, 清水 祥一, 日本農藝化學會誌,   1988 03
  • 微生物の制御培養(細胞培養工学をめぐる最近の諸問題), 清水 祥一, 山根 恒夫, 鈴木 高広, 日本農藝化學會誌,   1988 03
  • Mass production of lipase by fed-batch culture of Pseudomos sp., Yamane Tsuneo, Suzuki Takahiro, Mushiga Yoshinao, Ishihara Kazuto, Shimizu Shoichi, 日本醗酵工学会大会講演要旨集,   1987 11 02
  • Control of molecular weight of poly-β-hydroxybutyric acid produced in fed-batch culture of Protomonas extorquens., Suzuki Takahiro, Deguchi Hiroyuki, Yamane Tsuneo, Shimizu Shoichi, Gekko Kunihiko, 日本醗酵工学会大会講演要旨集,   1987 11 02
  • Mass production of thiostrepton by fed-batch culture of Streptomyces Laurentii with pH-stat modal feeding of multi-substrate., Suzuki Takahiro, Yamane Tsuneo, Shimizu Shoichi, 日本醗酵工学会昭和61年度大会講演要旨集,   1986 11
  • Detection and automatic control of ammonium ion concentration in microbial culture with ammonium ion selective electrode., Suzuki Takahiro, Yasuda Takashi, Yamane Tsuneo, Shimizu Shoichi, 日本醗酵工学会昭和60年度大会講演要旨集,   1985 10
  • Production of poly-β-hydroxybutyrate from methanol by microorganism, Suzuki Takahiro, Yamane Tsuneo, Shimizu Shoichi, 日本醗酵工学会昭和59年度大会講演要旨集,   1984 11
  • 247 微生物の高菌体濃度培養における金属イオン濃度の制御, 鈴木 高広, 森 博徳, 山根 恒夫, 清水 祥一, 日本醗酵工学会昭和58年度大会講演要旨集,   1983 11

Misc

  • 粉体化粧料の材料設計から加工プロセス開発まで 第4回 保湿剤が肌の水分蒸散を加速するメカニズムの解析と粉体化粧品によるバリア機能, 鈴木高広, 化学装置, 61, 11, 154‐157,   2019 11 01 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902279647244328
  • 感性に訴える化粧品テクスチャーの設計 官能評価と物性値を結びつけた化粧品粉体の客観的な使用感評価, 鈴木高広, 隔月刊Cosmetic Stage, 14, 1, 28‐36,   2019 10 25 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902218805978104
  • 地球環境とバイオリアクター~基礎から応用まで〈第48回〉最近の大地震における地殻ポンピング運動と低気圧の影響解析に基づく今後の発生予測と対策, 鈴木高広, 化学装置, 61, 9, 72‐75,   2019 09 01 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902247270501494
  • 医薬・化粧品製造で活躍する造粒・成形・乾燥技術 化粧品の最近の市場動向と技術傾向, 鈴木高広, 化学装置, 61, 7, 17‐25,   2019 07 01 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902265044585553
  • 粉体化粧料の材料設計から加工プロセス開発まで 第3回 切り餅を用いた化粧品の保湿効果試験法, 鈴木高広, 化学装置, 61, 6, 71‐75,   2019 06 01 , http://jglobal.jst.go.jp/public/201902286654350790
  • 地球環境とバイオリアクター~基礎から応用まで~〈第47回〉地殻ポンピング運動による大地震発生予測(地殻ポンピング運動が誘発する福島沖を震源とする巨大地震の発生予測モデル), 鈴木高広, 化学装置, 61, 5, 61‐65,   2019 05 01 , http://jglobal.jst.go.jp/public/201902234383448325
  • 粉体化粧料の材料設計から加工プロセス開発まで 第2回 肌水分率計の応答特性とファンデーション塗膜の接触角の影響, 鈴木高広, 化学装置, 61, 4, 64‐68,   2019 04 01 , http://jglobal.jst.go.jp/public/201902220497503402
  • 地球環境とバイオリアクター~基礎から応用まで~〈第46回〉地球温暖化が誘発した東北地方太平洋沖M9地震(後編)北海道胆振東部地震における地殻ポンピング運動と台風21号による起動作用, 鈴木高広, 化学装置, 61, 3, 67‐71,   2019 03 01 , http://jglobal.jst.go.jp/public/201902265946786478
  • 粉体化粧料の材料設計から加工プロセス開発まで―1 第1回 ブルーライトによる眼精疲労と肌の老化促進作用, 鈴木高広, 化学装置, 61, 1, 72‐76,   2019 01 01 , http://jglobal.jst.go.jp/public/201902257311037586
  • 地球環境とバイオリアクター~基礎から応用まで~〈第45回〉地球温暖化が誘発した東北地方太平洋沖M9地震(中編)北海道胆振東部地震における地殻ポンピング運動と台風21号による起動作用, 鈴木高広, 化学装置, 60, 12, 62‐67,   2018 12 01 , http://jglobal.jst.go.jp/public/201802260728267519
  • Current status and issues of development of potato energy aiming to realize RE 100 society, 鈴木高広, 坂本勝, 太陽/風力エネルギー講演論文集, 2018 (CD-ROM), ROMBUNNO.87,   2018 11 07 , http://jglobal.jst.go.jp/public/201802230111052084
  • Analysis of the mechanism of huge earthquake occurrence due to crustal pumping motion caused by global warming and analysis of photosynthesis efficiency of sweetpotato by solar sharing farming method, 鈴木高広, 坂本勝, 太陽/風力エネルギー講演論文集, 2018 (CD-ROM), ROMBUNNO.73,   2018 11 07 , http://jglobal.jst.go.jp/public/201802239833276905
  • 地球環境とバイオリアクター~基礎から応用まで~〈第44回〉地球温暖化が誘発した東北地方太平洋沖M9地震(前編)海面上昇と積雪量増大により規模を拡大する地殻ポンピング運動と巨大地震, 鈴木高広, 化学装置, 60, 10, 71‐77,   2018 10 01 , http://jglobal.jst.go.jp/public/201802217616613368
  • 地球環境とバイオリアクター~基礎から応用まで~〈第43回〉イモ燃料の大量生産技術開発―6 ソーラーシェアリング・空中栽培ハイブリッドシステムの開発, 鈴木高広, 化学装置, 60, 9, 64‐69,   2018 09 01 , http://jglobal.jst.go.jp/public/201802272228945498
  • 化粧品の最近の市場動向と技術傾向, 鈴木高広, 化学装置, 60, 7, 70, 78,   2018 07 01 , 招待有り, http://jglobal.jst.go.jp/public/201802221671295853
  • 地球環境とバイオリアクター~基礎から応用まで~〈第42回〉地球温暖化を加速する新興国の経済成長, 鈴木高広, 化学装置, 60, 6, 69‐73,   2018 06 , 招待有り, http://jglobal.jst.go.jp/public/201802221601324327
  • 地球環境とバイオリアクター~基礎から応用まで~〈第41回〉太陽光発電設備の過剰供給による温暖化加速問題と風力発電の現状, 鈴木高広, 化学装置, 60, 5, 75‐79,   2018 05 , 招待有り, http://jglobal.jst.go.jp/public/201802271933135378
  • 地球環境とバイオリアクター~基礎から応用まで~〈第40回〉再生可能エネルギーとスマートエネルギーの技術動向, 鈴木高広, 化学装置, 60, 4, 71‐76,   2018 04 , 招待有り, http://jglobal.jst.go.jp/public/201802280761806370
  • 地球環境とバイオリアクター~基礎から応用まで~〈第39回〉イモ燃料の大量生産技術開発―5;各種作物のバイオマス生産性と光合成効率の比較, 鈴木高広, 化学装置, 60, 3, 68‐71,   2018 03 , 招待有り, http://jglobal.jst.go.jp/public/201802227655109374
  • 化粧品の触り心地・つけ心地の制御と評価 化粧品における心地・触り心地の制御・改良方法, 鈴木高広, 隔月刊Cosmetic Stage, 12, 3, 35‐43,   2018 02 , 招待有り, http://jglobal.jst.go.jp/public/201802231707660753
  • Development of a composite powder processing process to prevent ultraviolet rays and near infrared rays and improve the feeling of use, 鈴木 高広, Fragrance journal : Research & development for cosmetics, toiletries & allied industries = フレグランスジャーナル : 香粧品科学研究開発専門誌, 45, 10, 18, 24,   2017 10 , http://ci.nii.ac.jp/naid/40021364600
  • 地球環境とバイオリアクター~基礎から応用まで~〈第32回〉燃料作物農業の格安量産技術が高める食料自給率, 鈴木高広, 化学装置, 59, 8, 65‐69, 69,   2017 08 , 招待有り, http://jglobal.jst.go.jp/public/201702271700359104
  • 地球環境とバイオリアクター~基礎から応用まで~〈第31回〉食料自給率と燃料作物経済, 鈴木高広, 化学装置, 59, 6, 75, 79,   2017 06 , 招待有り, http://jglobal.jst.go.jp/public/201702246811804619
  • 地球環境とバイオリアクター~基礎から応用まで~〈第30回〉再生可能エネルギーの技術動向とエネルギーフロー解析に基づく省エネ余力, 鈴木高広, 化学装置, 59, 5, 74, 78,   2017 05 , 招待有り, http://jglobal.jst.go.jp/public/201702213653723452
  • 地球環境とバイオリアクター~基礎から応用まで《第29回》地球温暖化が大地震や火山噴火を増大する日本列島風動説, 鈴木高広, 化学装置, 59, 4, 61, 66,   2017 04 , 招待有り, http://jglobal.jst.go.jp/public/201702268580157412
  • 地球環境とバイオリアクター~基礎から応用まで~《第28回》地球温暖化により急増する大地震と火山噴火, 鈴木高広, 化学装置, 59, 3, 58, 62,   2017 03 , 招待有り, http://jglobal.jst.go.jp/public/201702233966247407
  • 地球環境とバイオリアクター~基礎から応用まで~〈第27回〉自然メタン発生の増大と小型メタンガスファームシステムによる抑止, 鈴木高広, 化学装置, 59, 2, 59, 64,   2017 02 , 招待有り, http://jglobal.jst.go.jp/public/201702238720239662
  • 地球環境とバイオリアクター~基礎から応用まで~〈第26回〉温室効果ガスの温暖化係数と加速する地球温暖化スパイラル, 鈴木高広, 化学装置, 59, 1, 77, 81,   2017 01 , 招待有り, http://jglobal.jst.go.jp/public/201702249157541443
  • 地球環境とバイオリアクター~基礎から応用まで~〈第25回〉ペットボトルメタン発酵槽を用いたイモメタンガス製造プロセス, 鈴木高広, 化学装置, 58, 12, 67, 73,   2016 12 , 招待有り, http://jglobal.jst.go.jp/public/201602231253111352
  • 地球環境とバイオリアクター~基礎から応用まで~〈第24回〉バイオマスゴミのリサイクル研究とバイオマスエネルギー研究の区別, 鈴木高広, 化学装置, 58, 11, 123, 128,   2016 11 , 招待有り, http://jglobal.jst.go.jp/public/201602272128722297
  • 地球環境とバイオリアクター~基礎から応用まで~〈第23回〉日本と世界の森林資源の動向と木質バイオマス発電の電力調達価格, 鈴木高広, 化学装置, 58, 10, 101, 106,   2016 10 , 招待有り, http://jglobal.jst.go.jp/public/201602220487642750
  • 地球環境とバイオリアクター~基礎から応用まで~〈第22回〉木質バイオマス発電が地球温暖化を加速する, 鈴木高広, 化学装置, 58, 9, 59, 64,   2016 09 , 招待有り, http://jglobal.jst.go.jp/public/201602221214394088
  • 地球環境とバイオリアクター~基礎から応用まで~〈第21回〉国内の燃料作物農業創出への課題, 鈴木高広, 化学装置, 58, 8, 68, 72,   2016 08 , 招待有り, http://jglobal.jst.go.jp/public/201602259903022804
  • 地球環境とバイオリアクター~基礎から応用まで~〈第20回〉ドイツと日本におけるバイオガス発電の現状, 鈴木高広, 化学装置, 58, 6, 70, 74,   2016 06 , 招待有り, http://jglobal.jst.go.jp/public/201602283382740409
  • 地球環境とバイオリアクター~基礎から応用まで~〈第18回〉ソーラー発電設備のEPT値の疑問点と温暖化影響の解消年数, 鈴木高広, 化学装置, 58, 4, 67, 71,   2016 04 , 招待有り, http://jglobal.jst.go.jp/public/201602200141397989
  • 地球環境とバイオリアクター~基礎から応用まで~〈第16回〉世界の再生可能エネルギーの導入による化石燃料代替効果の現状, 鈴木高広, 化学装置, 58, 2, 68, 72,   2016 02 , 招待有り, http://jglobal.jst.go.jp/public/201602220972177951
  • 地球環境とバイオリアクター~基礎から応用まで~〈第15回〉再生可能エネルギーの導入による化石燃料代替効果の現状, 鈴木高広, 化学装置, 58, 1, 62, 66,   2016 01 , 招待有り, http://jglobal.jst.go.jp/public/201602210215025138
  • 地球環境とバイオリアクター~基礎から応用まで~《第14回》化石燃料消費に伴う鍋蓋効果ガスの増大, 鈴木高広, 化学装置, 57, 12, 68, 72,   2015 12 , 招待有り, http://jglobal.jst.go.jp/public/201502214317311451
  • 地球環境とバイオリアクター~基礎から応用まで~〈第13回〉日本のバイオマスエネルギー基本計画とエネルギー学会の動向, 鈴木高広, 化学装置, 57, 10, 79, 83,   2015 10 , 招待有り, http://ci.nii.ac.jp/naid/40020601335
  • サンケア化粧品の抗紫外線防止効果の付与と使用感の両立 UVファンデーションにおける使用感と紫外線防止効果との両立, 鈴木高広, Cosmetic Stage, 9, 7, 40‐47,   2015 10 , 招待有り, http://jglobal.jst.go.jp/public/201602200370461666
  • 地球環境とバイオリアクター~基礎から応用まで~〈第12回〉森林バイオマスエネルギー開発の真実, 鈴木高広, 化学装置, 57, 9, 68, 71,   2015 09 , 招待有り, http://ci.nii.ac.jp/naid/40020571539
  • Development of composite UV-screening flake powder and the improvement of use sense of make-up cosmetics, 鈴木 高広, Fragrance Journal フレグランスジャーナル, 43, 6, 53, 62,   2015 06 , 招待有り, http://ci.nii.ac.jp/naid/40020512072
  • 地球環境とバイオリアクター~基礎から応用まで~〈第10回〉フォトバイオリアクターと光合成ビジネス, SUZUKI TAKAHIRO, 化学装置, 57, 6, 65, 68,   2015 06 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502290034369230
  • A practical application of dispersion use of sunlight in planter cultivation method for agricultural reconstruction assistance in Fukushima, 鈴木 高広, 堀端 章, 坂本 勝, 太陽/風力エネルギー講演論文集, 455, 458,   2015 , http://ci.nii.ac.jp/naid/40020678849
  • 1P-214 Growth properties of sweet potato in the triangle shelf multi-layer cultivation method and its methane fermentation condition, Suzuki Takahiro, Sakamoto Masaru, Ano Takashi, Shintani Noboru, 日本生物工学会大会講演要旨集, 65,   2013 08 25 , http://ci.nii.ac.jp/naid/110009737594
  • 医薬品・化粧品製造における化学技術・粉体技術の役割 化粧品の最近の市場動向と技術傾向, 鈴木高広, 化学装置, 55, 7, 49, 55,   2013 07 , 招待有り, http://jglobal.jst.go.jp/public/201302277048406617
  • 日本を救う芋エネルギー―新たなエネルギービジネスの展開戦略―, SUZUKI TAKAHIRO, 土地改良, 51, 2, 2, 14,   2013 04 , 招待有り, http://ci.nii.ac.jp/naid/40019677529
  • 湿式パウダー製剤の成型のコツ, 鈴木高広, Cosmetic Stage, 7, 2, 39, 47,   2012 12 , 招待有り, http://ci.nii.ac.jp/naid/40019539436
  • パウダーファンデーション化粧品の低硬度化と紫外線遮蔽度の改良技術, 野村享平, 鈴木高広, 粉体工学会研究発表会講演論文集, 2012, 103, 104,   2012 11 , http://jglobal.jst.go.jp/public/201302282567689662
  • Visualization of Influence of Pulverization Process of Cosmetic Powders on Feeling on Use, SUZUKI Takahiro, UMEDA Tomohiro, 粉体工学会誌, 49, 3, 221, 225,   2012 03 , Refereed, 10.4164/sptj.49.221, http://ci.nii.ac.jp/naid/10030479344
  • Simple Evaluation Method for Long-Lasting of Foundation, Cosmetic stage, 6, 2, 16, 28,   2011 12 , 招待有り, http://ci.nii.ac.jp/naid/40019202653
  • Pulverization process of powder raw materials and visualization of its influence in use sense of cosmetics on skin, 鈴木高広, Cosmetic stage, 6, 1, 37, 46,   2011 10 , 招待有り, http://ci.nii.ac.jp/naid/40019043338
  • 化粧品用粉体原料の肌面使用感に対する粉砕の影響と可視化, SUZUKI TAKAHIRO, UMEDA TOMOHIRO, 粉体工学会夏期シンポジウム講演論文集, 47th, 15, 16,   2011 08 08 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102238412726622
  • 化粧品の嗜好性分析 扁桃体機能と系統的脱感作療法の化粧品への応用に関する考察, YOSHIDA TAKAYOSHI, UMEDA TOMOHIRO, SUZUKI TAKAHIRO, Cosmetic Stage, 5, 8, 6, 10,   2011 08 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102238736843451
  • 化粧品の嗜好性分析 快適感とストレスの心電図遠隔計測システム, UMEDA TOMOHIRO, YOSHIDA TAKAYOSHI, SUZUKI TAKAHIRO, Cosmetic Stage, 5, 8, 11, 18,   2011 08 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102232514652790
  • 化粧品の嗜好性分析 化粧品の技術情報データベースの構築と無意識に最適商品を手にするアンビエント技術, SUZUKI TAKAHIRO, YOSHIDA TAKAYOSHI, UMEDA TOMOHIRO, Cosmetic Stage, 5, 8, 19, 30,   2011 08 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102219416973832
  • Observation of nano particles of cosmetics of the market applied on skin, SUZUKI TAKAHIRO, UMEDA TOMOHIRO, Cosmetic stage, 5, 2, 45, 55,   2010 12 , 招待有り, http://ci.nii.ac.jp/naid/40017446581
  • 粉体ナノテクノロジー 第6章 ナノ粒子・ナノ物質の安全な利用 6.6 化粧品におけるナノ粒子剤の役割と安全性に関する現状, SUZUKI TAKAHIRO, 粉体工学会誌, 47, 12, 830, 838,   2010 12 , Refereed, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102282951222637
  • 香粧品の使用感に関する研究開発の最前線 化粧品の嗜好性の解析技術と市場のトレンド操作のための知識工学的アプローチ, SUZUKI TAKAHIRO, Cosmetic Stage, 4, 8, 9, 15,   2010 08 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002231993303580
  • 香粧品の使用感に関する研究開発の最前線 低真空走査型電子顕微鏡を用いたパウダー化粧品の使用感物性の可視化と湿式充填品の解析, SUZUKI TAKAHIRO, UMEDA TOMOHIRO, Cosmetic Stage, 4, 8, 16, 24,   2010 08 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002273596734730
  • 【ファンデーション】剤型ごとの処方設計の最新トレンド 強度と感触のバランスを考慮したパウダーファンデーションの処方設計, SUZUKI TAKAHIRO, Cosmetic Stage, 4, 4, 24, 28,   2010 02 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002294103451635
  • Application of sensorial evaluation data and instrumental measurements in development of powder cosmetic products, SUZUKI TAKAHIRO, Fragrance Journal, 37, 12, 49, 57,   2009 12 , 招待有り, http://ci.nii.ac.jp/naid/40016935386
  • 業種別に見る粉体技術の活用 化粧品分野における粉砕技術, SUZUKI TAKAHIRO, 化学装置, 51, 5, 33, 42,   2009 05 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902207214712534
  • Sensorial and in vitro evaluation methods for development of powder products, Cosmetic stage, 3, 1, 1, 6,   2008 10 , 招待有り, http://ci.nii.ac.jp/naid/40016314197
  • Flake particle technology in make-up product, SUZUKI TAKAHIRO, Cosmetic stage, 2, 4, 55, 62,   2008 04 , 招待有り, http://ci.nii.ac.jp/naid/40016069209
  • Development of aeropowder foundation., DUMOUSSEAUX CHRISTOPHE, KAWAMOTO MAKOTO, KANI TOSHIYUKI, GOTO TATSUNARI, SUZUKI TAKAHIRO, Fragrance Journal, 34, 6, 40, 48,   2006 06 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902275982426333
  • Application of optical properties for make-up function. Improvement of transparency and luster of synthetic mica powder for cosmetics., SUZUKI TAKAHIRO, Fragrance Journal, 30, 4, 45, 52,   2002 04 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902152329033240
  • Continuous production of lactic acid from molasses., Kasahara Norihide, Ueno Shigeaki, Ohashi Ryo, Suzuki Takahiro, 日本生物工学会大会講演要旨集, 11, 274, 274,   1999 , http://dl.ndl.go.jp/info:ndljp/pid/10523904
  • Dense cell culture technology of microorganisms using a shake flask, SUZUKI Takahiro, 55, 9, 33, 34,   1997 09 , 招待有り, http://ci.nii.ac.jp/naid/10007295523
  • 振盪フラスコで乾燥菌体濃度200g/lを達成する灌流培養技術の開発, 鈴木 高広, 鴨下 祐也, 大橋 亮, 化学工学, 61, 8, 610, 611,   1997 08 , 招待有り, http://ci.nii.ac.jp/naid/10003809717
  • Research on surface functional ceramics for bio-functional control. ( Agency of Industrial Science and Technology S ), TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, YOKOGAWA YOSHIYUKI, NISHIZAWA KAORI, NAGATA FUKUE, KAMEYAMA TETSUYA, 通商産業省工業技術院特別研究報告集, 1996, 35, 38,   1996 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902110675464849
  • Morphology of Hydroxyapatite Crystals Synthesized in the Presence of Organic Solution. (3)., NAGATA FUKUE, YOKOGAWA YOSHIYUKI, TORIYAMA MOTOHIRO, KAWAMOTO YUKARI, SUZUKI TAKAHIRO, NISHIZAWA KAORI, 日本セラミックス協会年会講演予稿集, 1995, 114,   1995 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902158985312600
  • " Raise " cells on ceramics.About bioreactor research., SUZUKI TAKAHIRO, バウンダリー, 7, 3, 55, 59,   1991 03 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902048833203961
  • バイオリアクター材料としての機能性セラミックス, 鈴木高広, 醗酵工学会誌, 68, 5, 431,   1990 09 , 招待有り, http://ci.nii.ac.jp/naid/110002778485
  • Production of poly-beta-hydroxybutyric acid from methanol by microorganisms., SHIMIZU SHOICHI, SUZUKI TAKAHIRO, 発酵と工業, 45, 11, 1080, 1087,   1987 11 , 招待有り, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902027876065204
  • Optical gaps of alkali borate and alkali fluoroborate glasses, T Suzuki, M Hirano, H Hosono, JOURNAL OF APPLIED PHYSICS, 91, 7, 4149, 4153,   2002 04 , 10.1063/1.1456946
    Summary:The optical gaps of B2O3 alkali borate, and alkali fluoroborate glasses were determined by using blown films. The gap of B2O3 glass was 8.0 eV and the value decreased monotonically with decreasing the B2O3 content in both fluoroborate and borate glasses. When compared at a constant B2O3 content, the gap is in the order Li>Na>K and fluoroborate>borate. Ab initio molecular orbital calculation on the model clusters of the glasses revealed that the lowest excitations in B2O3 and alkali (fluoro) borate glasses are the transitions from nonbonding 2p orbitals of oxygen atoms in boroxol rings to vacant nonbonding 2p orbitals of boron atoms on "loose" BO3 triangles and from nonbonding 2p orbitals of oxygen atoms bonded with borons in tetrahedral coordination to the vacant ns orbitals of neighboring alkali ions, respectively. The vacant ns orbitals of the alkali ions neighboring oxygen atoms attached to tetrahedral borons primarily contribute to the lowest unoccupied molecular orbital (LUMO) levels. On the other hand, fluorine indirectly contributes to lowering the highest occupied molecular orbital (HOMO) levels via inductive effect (polarization of B-F sigma-bond). It was therefore concluded that the effect of alkali addition on lowering LUMO levels is larger than that of fluorine addition on lowering the HOMO level. (C) 2002 American Institute of Physics.
  • PROTEIN-PRODUCTION BY LYSIS WITH GLYCINE OF A FACULTATIVE METHYLOTROPH, PROTAMINOBACTER-RUBER, CULTIVATED IN A FED-BATCH SYSTEM, K SATO, K MARUYAMA, H MORI, T SUZUKI, S SHIMIZU, HAKKOKOGAKU KAISHI-JOURNAL OF THE SOCIETY OF FERMENTATION TECHNOLOGY, 62, 3, 301, 303,   1984