SHIMIZU Kosuke

    Experimental Farm Lecturer
Last Updated :2024/03/24

Researcher Information

Degree

  • Doctor of Agriculture(2021/09 Kindai University)

J-Global ID

Research Areas

  • Environmental science/Agricultural science / Horticulture

Academic & Professional Experience

  • 2022/04 - Today  Kindai UniversityExperimental Farms講師
  • 2007/04 - 2021/03  Kindai UniversityExperimental Farms助教
  • 2002/04 - 2007/03  Kindai UniversityExperimental Farms助手

Education

  • 2000/04 - 2002/03  Kindai University  農学部農学研究科農学専攻

Association Memberships

  • JAPAN SOCIETY OF ENERGY AND RESOURCES   JAPANESE SOCIETY FOR TROPICAL AGRICULTURE   THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE   

Published Papers

  • 志水恒介; 井田民男
    スマートプロセス学会誌 12 (1) 2186-702X 2023
  • Kosuke Shimizu; Tetsuya Matsukawa; Risa Kanematsu; Kimihisa Itoh; Shinya Kanzaki; Shigeru Shigeoka; Shin'ichiro Kajiyama
    Bioscience, Biotechnology, and Biochemistry Oxford University Press (OUP) 85 (8) 1789 - 1797 2021/07 [Refereed]
     
    ABSTRACT Headspace solid-phase microextraction combined with gas chromatography/mass spectrometry is one of the strongest tools for comprehensive analysis of volatile compounds and has been used to analyze aromatic components of mango and investigate its varietal characteristics. In this study, profiling of aroma compounds in 17 mango cultivars, grown in the same green house to exclude the effect of environmental factors, was conducted and the patterns were subjected to principal component analysis (PCA) to identify the relationship between the aroma components and cultivars. Fifty-nine different volatile constituents were detected from the blends of these 17 mango cultivars. The cultivars were divided into 4 clusters using PCA based on the volatile components determined in the study. Aiko was found to mainly contain δ-3-carene and showed a composition more similar to its pollen parent, Irwin, than to its seed parent, Chiin Hwang No. 1.
  • Shimizu Kosuke; Tatsumi Kentaro; Utsunomiya Naoki; Kanzaki Shinya; Itoh Kimihisa; Shigeoka Shigeru
    Horticultural Research (Japan) THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 20 (1) 87 - 94 1347-2658 2021/01 [Refereed]
     
    Mango ‘Aiko’ is a large-fruited and medium-late cultivar that has a stronger biennial-bearing nature than ‘Irwin’. In this study, we applied the intentional alternate bearing method, which has been practiced in citrus cultivation, to mango ‘Aiko’ and examined its applicability. Two treatments, alternate branch unit bearing and whole-tree annual bearing, were set up and compared with the control of the conventional method. Although there was no significant difference in the proportion of reproductive shoots between the control and two treatments, the proportion of reproductive shoots on ON-trees or ON-branches was consistently high. No significant difference was observed in yield between the control and treatments, while the mean fruit weight was significantly lower in both treatments compared with control fruits. The percentage of small fruits was higher in both treatments. With respect to work efficiency, it was shown that both alternate bearing methods could reduce the time required to prune a tree. On the other hand, the expression level of the MiFT gene was shown to be related to the biennial-bearing nature of ‘Aiko’, suggesting that it could be used to predict the number of flowers in the following season. Our results show that the intentional alternate bearing method for mango ‘Aiko’ is an effective method to control the fluctuation of yield and reduce labor while maintaining the same level of fruit quality and yield as the conventional method.
  • 志水恒介; 巽賢太郎; 宇都宮直樹; 神崎真哉; 伊藤仁久; 重岡成
    熱帯農業研究 Japanese Society for Tropical Agriculture 14 (1) 19 - 25 1882-8434 2021 [Refereed]
     
    Recently, mangoes are cultivated in various regions in Japan, but domestic cultivation still depends on one cultivar, ‘Irwin’. ‘Aiko’, a new cultivar bred in Kindai University, is characterized by larger fruits, less pulp fiber, later harvesting time than ‘Irwin’, and expected as an alternative cultivar to ‘Irwin’. As ‘Aiko’ has strong biennial-bearing tendency, it’s necessary to clarify flowering characteristics to establish a cultivation management suitable for ‘Aiko’. In this study, we investigated the effects of the previous year’s fruiting and the number of flushes on the proportion of reproductive shoot. Furthermore, the expression profile of MiFT gene was analyzed. The results showed that ‘Aiko’ has lower floral inducibility than ‘Irwin’, and that the previous year’s fruiting strongly influenced the proportion of reproductive shoot in ‘Aiko’. In both cultivars, the number of flushes was higher in unfruited branches than in fruited branches. The proportion of reproductive shoot in ‘Irwin’ was high regardless of the number of flushes, while that in ‘Aiko’ was higher for branches with more frequent shoot expansions. MiFT expression was hardly affected by the intensity of crop load and was higher in ‘Irwin’ than in ‘Aiko’. In ‘Aiko’, it was shown that it is important to secure a shoot that has flushed more than three times after pruning. Thus, the cultivation method like intentional biennial bearing method, in which productive and non-productive years are clearly distinguished, should be considered for ‘Aiko’.
  • Shinya Kanzaki; Asuka Ichihi; Yuta Tanaka; Shiina Fujishige; Sota Koeda; Kosuke Shimizu
    SCIENTIA HORTICULTURAE ELSEVIER 272 109567 - 109567 0304-4238 2020/10 [Refereed]
     
    The skin color of mature mango (Mangifera indica L.) fruit varies from green to red depending on cultivars. Red coloration of mango fruit skin is due to anthocyanin accumulation and is known to be induced by light exposure as in some other fruit crops. Recently, several investigations on the genes involved in anthocyanin biosynthesis in mango fruit skin have been conducted, to understand the molecular mechanism underlying red coloration of the mango fruit. In the present study, we investigated the characteristics of four R2R3-MYB transcription factors (TF) including MiMYB1, which is assumed to be the MYB TF regulating anthocyanin biosynthesis in mangoes. The deduced amino acid sequence of MiMYB1 showed a high degree of similarity with the anthocyanin-related R2R3-MYB TFs from other plant species and contained two conserved motifs defining the anthocyanin-related MYB TFs. Expression analysis in the mango fruit skin under different light conditions showed that the expression of MiMYB1 increased as the intensity of light exposure increased, parallel to anthocyanin accumulation and the expression of some structural genes of the anthocyanin biosynthetic pathway. In addition, a transient expression assay of tobacco leaves showed that co-infiltration of MiMYB1 and MibHLH2, which is a bHLH TF of mango, induced anthocyanin accumulation in tobacco leaves. These results suggest that MiMYB1 acts as the key regulator for anthocyanin biosynthesis in mango fruit skin, and that light-dependent red coloration of mango fruit skin is regulated by MiMYB1 in transcript levels.
  • Kimihisa Itoh; Tetsuya Matsukawa; Mamoru Okamoto; Kanasa Minami; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka
    Journal of Plant Studies Canadian Center of Science and Education 9 (2) 39 - 39 1927-0461 2020/06 [Refereed]
     
    In this study, we aimed to identify the utility of pruned mango (Mangifera indica ‘Irwin’) leaves as a resource for ingredients with antioxidant activity. Firstly, we examined the antioxidant activity of extracts obtained from the pericarps, flesh, flowers, barks, seeds, young dark reddish brown leaves (YDL-ext), young yellow leaves (YYL-ext), and pruned old dark green leaves (OML-ext) obtained from ‘Irwin’ mango. Among them, methanolic extract of flower and OML-ext showed the most potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity. The flesh extract showed weak DPPH radical scavenging activity, but did not show SOD-like activity. Secondly, we investigated the relationship between the maturation of leaves and their antioxidant activity by considering the contents of their two active polyphenolic components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1) and mangiferin (2), in addition to chlorophyll (3) and anthocyanins represented by cyanidin-3-O-glucoside (4). The DPPH radical scavenging activity of YDL-ext, YYL-ext and OML-ext were mainly attributable to 1, 2 and 3, whereas their SOD-like activity was partly attributable to 2. The DPPH radical scavenging and SOD-like activities of YDL-ext and YYL-ext were attributable to 1 and 2. These activities were also due to anthocyanins whose content is highest in YDL-ext. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for preparing cosmetics and/or supplemental ingredients with health-enhancing properties, antioxidant activity and inhibitory effect on AGEs formation and pancreatic lipase.
  • Kimihisa Itoh; Tetsuya Matsukawa; Kanasa Minami; Mamoru Okamoto; Norimichi Tomohiro; Kosuke Shimizu; Shin’ichiro Kajiyama; Yuichi Endo; Hideaki Matsuda; Shigeru Shigeoka
    Journal of Plant Studies Canadian Center of Science and Education 9 (2) 33 - 33 1927-0461 2020/05 [Refereed]
     
    As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation.
  • Ayako Katayama-Ikegami; Zion Byun; Suzuka Okada; Masahiro Miyashita; Takane Katayama; Tomoaki Sakamoto; Asuka Ichihi; Kosuke Shimizu; Shinya Kanzaki
    HORTICULTURE JOURNAL JAPAN SOC HORTICULTURAL SCI 89 (5) 516 - 524 2189-0102 2020 [Refereed]
     
    Red mangos (Mangifera indica L.) accumulate appreciable amounts of cyanidin-based anthocyanins in the skin, and previous studies showed that these anthocyanins contain galactose as a sugar moiety. To date, two UDP:flavonoid 3-O-glycosyltransferase (UFGT)-like genes named MiUFGT1 and MiUFGT3 (MiUFGalT3) have been isolated from mango `Irwin' peel as anthocyanin-related UFGT genes, but the function of the proteins of the genes have not yet been elucidated. In this study, we characterized recombinant MiUFGT1 and MiUFGalT3 expressed in Escherichia coli. In the presence of quercetin as an acceptor, rMiUFGT1 showed marginal glucosylation activity, while rMiUFGalT3 exhibited significant galactosylation activity 20-fold higher than its glucosylation activity. Specificity analysis using purified MiUFGalT3 found that rMiUFGalT3 almost equally accepts anthocyanidins and flavonols. The anthocyanins extracted from the `Irwin' skins were cyanidin 3-O-galactoside and 7-O-methylcyanidin 3-O-galactoside by instrumental analyses, which is consistent with previous results obtained for other red mango cultivars. The results suggest that MiUFGalT3 is responsible for the red coloration of `Irwin' mango fruit skins.
  • Shinya Kanzaki; Shiori Kamikawa; Asuka Ichihi; Yuta Tanaka; Kosuke Shimizu; Sota Koeda; Naoki Utsunomiya
    HORTICULTURE JOURNAL JAPAN SOC HORTICULTURAL SCI 88 (4) 435 - 443 2189-0102 2019 [Refereed]
     
    The red coloration of the mango 'Irwin' skin is an important factor determining its value in the Japanese domestic luxury fruit market. In the present study, to investigate the molecular mechanism underlying anthocyanin biosynthesis of mango fruit skin, UFGT-like genes were isolated and the expression profile of anthocyanin-related genes was determined. Several UFGT-like genes were identified in transcriptome data of red 'Irwin' mango skin and two genes, MiUFGT1 and MiUFGT3, were considered to be involved in mango skin coloration. Deduced amino acid sequences of these genes exhibited high similarity to other plant UFGTs and contained the conserved PSPG box common to the glycosyltransferase family. The presence of a glutamine and a histidine residue at the C-terminus end of the PSPG box in MiUFGT1 and MiUFGT3, respectively, implied that MiUFGT1 and MiUFGT3 use glucose and galactose, respectively, as a sugar donor; however, the actual function and sugar donor preference of these enzymes remain to be elucidated. Expression analysis of anthocyanin-related genes during skin coloration suggested that MiCHS and MiANS, as well as MiUFGT1 and MiUFGT3, play important roles in the anthocyanin biosynthesis of mango fruit skin and that the expression of these genes is regulated by the MYB transcription factor, as reported in other plant species.
  • Kimihisa Itoh; Kazuya Murata; Nao Sakaguchi; Kohei Akai; Tomoka Yamaji; Kohsuke Shimizu; Kaoru Isaki; Tetsuya Matsukawa; Shinichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki Matsuda
    Journal of Plant Studies Canadian Center of Science and Education 6 (2) 102 - 102 1927-0461 2017/08 [Refereed]
     
    The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.
  • Kimihisa Itoh; Kazuya Murata; Yuta Nakagaki; Ayaka Shimizu; Yusuke Takata; Kohsuke Shimizu; Tetsuya Matsukawa; Shin'ichiro Kajiyama; Masahiko Fumuro; Morio Iijima; Hideaki Matsuda
    Journal of Plant Studies 5 (2) 72 - 78 2016/06 [Refereed]
  • Masahiro Nakagawa; Chitose Honsho; Shinya Kanzaki; Kousuke Shimizu; Naoki Utsunomiya
    SCIENTIA HORTICULTURAE ELSEVIER 139 108 - 117 0304-4238 2012/05 [Refereed]
     
    Mango (Mangifera indica L.) is one of the most important evergreen fruit trees, but it has a high tendency of biennial bearing because of irregular flowering. In this study, a FLOWERING LOCUS T-like (FT-like) gene was isolated from mango (MiFT) and characterized. The deduced amino acid sequence of MiFT showed high identity of the gene to other plant FT-like genes, and further, MiFT expression increased only in the leaves under floral-inductive conditions. Comparison of heavy crop load (HC) and no crop load (NC) mango trees showed that MiFT expression strongly increased only in NC trees under cool temperature. In NC trees, almost all the apical buds formed panicles, whereas in HC trees, only a few panicles were produced in the next season of fruit set. Further. HC trees had lower starch content in the shoots than NC trees. Furthermore, application of 250-ppm gibberellin 3 (GA(3)) completely inhibited flowering and MiFT expression in both HC and NC trees. GA metabolism genes were also isolated from mango and their expression patterns were investigated. Gibberellin-3-oxidase (GA3-ox) controls the final step of biosynthesis of active GA, and its gene expression surged only in HC trees under cool temperature. In conclusion, MiFT is considered a key factor in mango flowering, and regulation of MiFT expression through GA metabolism was discussed. (C) 2012 Elsevier B.V. All rights reserved.

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