KINDAI UNIVERSITY


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HAYASAKA Haruko

Profile

FacultyDepartment of Life Science / Graduate School of Science and Engineering Research
PositionAssociate Professor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/1375-hayasaka-haruko.html
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Last Updated :2020/04/03

Education and Career

Academic & Professional Experience

  •   2009 ,  - 2013 , Osaka University

Research Activities

Research Areas

  • Life sciences, Cell biology
  • Life sciences, Immunology

Research Interests

  • GPCR, HEV, MAdCAM-1

Published Papers

  • Endogenous membrane receptors labeling by reactive cytokines/growth factors to chase their dynamics in live cells, Takaoka Y, Uchinomiya S, Kobayashi D, Endo M, Hayashi T, Fukuyama Y, Hayasaka H, Miyasaka M, Ueda T, Shimada I, Hamachi I, Chem, Chem, 4(6), 1451 - 1464, Jun. 2018 , Refereed
  • Regulation of CCR7-dependent cell migration through CCR7 homodimer formation, Daichi Kobayashi, Masataka Endo, Hirotaka Ochi, Hironobu Hojo, Masayuki Miyasaka, Haruko Hayasaka, SCIENTIFIC REPORTS, SCIENTIFIC REPORTS, 7(1), 8536, Aug. 2017 , Refereed
    Summary:The chemokine receptor CCR7 contributes to various physiological and pathological processes including T cell maturation, T cell migration from the blood into secondary lymphoid tissues, and tumor cell metastasis to lymph nodes. Although a previous study suggested that the efficacy of CCR7 ligand-dependent T cell migration correlates with CCR7 homo- and heterodimer formation, the exact extent of contribution of the CCR7 dimerization remains unclear. Here, by inducing or disrupting CCR7 dimers, we demonstrated a direct contribution of CCR7 homodimerization to CCR7-dependent cell migration and signaling. Induction of stable CCR7 homodimerization resulted in enhanced CCR7-dependent cell migration and CCL19 binding, whereas induction of CXCR4/CCR7 heterodimerization did not. In contrast, dissociation of CCR7 homodimerization by a novel CCR7-derived synthetic peptide attenuated CCR7-dependent cell migration, ligand-dependent CCR7 internalization, ligand-induced actin rearrangement, and Akt and Erk signaling in CCR7-expressing cells. Our study indicates that CCR7 homodimerization critically regulates CCR7 ligand- dependent cell migration and intracellular signaling in multiple cell types.
  • Lysophosphatidic acid receptors LPA4 and LPA6 differentially promote lymphocyte transmigration across high endothelial venules in lymph nodes., Hata E, Sasaki N, Takeda A, Tohya K, Umemoto E, Akahoshi N, Ishii S, Bando K, Abe T, Kano K, Aoki J, Hayasaka H, Miyasaka M, International immunology, International immunology, 28(6), 283 - 292, Jun. 2016 , Refereed
  • Fibroblastic reticular cell-derived lysophosphatidic acid regulates confined intranodal T-cell motility., Takeda A, Kobayashi D, Aoi K, Sasaki N, Sugiura Y, Igarashi H, Tohya K, Inoue A, Hata E, Akahoshi N, Hayasaka H, Kikuta J, Scandella E, Ludewig B, Ishii S, Aoki J, Suematsu M, Ishii M, Takeda K, Jalkanen S, Miyasaka M, Umemoto E, eLife, eLife, 5, e10561, Feb. 2016 , Refereed
  • Divergence of Vascular Specification in Visceral Lymphoid Organs-Genetic Determinants and Differentiation Checkpoints., Kellermayer Z, Hayasaka H, Kajtár B, Simon D, Robles EF, Martinez-Climent JA, Balogh P, International reviews of immunology, International reviews of immunology, 35(6), 489 - 502, Jul. 2015 , Refereed
  • The HIV-1 Gp120/CXCR4 axis promotes CCR7 ligand-dependent CD4 T cell migration: CCR7 homo- and CCR7/CXCR4 hetero-oligomer formation as a possible mechanism for up-regulation of functional CCR7., Hayasaka H, Kobayashi D, Yoshimura H, Nakayama EE, Shioda T, Miyasaka M, PloS one, PloS one, 10(2), e0117454, 2015 , Refereed
  • Constitutive lymphocyte transmigration across the basal lamina of high endothelial venules is regulated by the autotaxin/lysophosphatidic acid axis., Bai Z, Cai L, Umemoto E, Takeda A, Tohya K, Komai Y, Veeraveedu PT, Hata E, Sugiura Y, Kubo A, Suematsu M, Hayasaka H, Okudaira S, Aoki J, Tanaka T, Albers HM, Ovaa H, Miyasaka M, Journal of immunology (Baltimore, Md. : 1950), Journal of immunology (Baltimore, Md. : 1950), 190(5), 2036 - 2048, Mar. 2013 , Refereed
  • Dynamic changes in endothelial cell adhesion molecule nepmucin/CD300LG expression under physiological and pathological conditions., Umemoto E, Takeda A, Jin S, Luo Z, Nakahogi N, Hayasaka H, Lee CM, Tanaka T, Miyasaka M, PloS one, PloS one, 8(12), e83681, 2013 , Refereed
  • Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling., Umemoto E, Otani K, Ikeno T, Verjan Garcia N, Hayasaka H, Bai Z, Jang MH, Tanaka T, Nagasawa T, Ueda K, Miyasaka M, Journal of immunology (Baltimore, Md. : 1950), Journal of immunology (Baltimore, Md. : 1950), 189(1), 191 - 199, Jul. 2012 , Refereed
  • [Regulation of immune cell and cancer cell trafficking by multiple chemokines]., Hayasaka H, Okada M, Bai Z, Kuroda Y, Yoshida J, Miyasaka M, Seikagaku. The Journal of Japanese Biochemical Society, Seikagaku. The Journal of Japanese Biochemical Society, 83(10), 930 - 937, Oct. 2011 , Refereed
  • Novel regulators of lymphocyte trafficking across high endothelial venules., Umemoto E, Hayasaka H, Bai Z, Cai L, Yonekura S, Peng X, Takeda A, Tohya K, Miyasaka M, Critical reviews in immunology, Critical reviews in immunology, 31(2), 147 - 169, 2011 , Refereed
  • CXC chemokine ligand 12 promotes CCR7-dependent naive T cell trafficking to lymph nodes and Peyer's patches., Bai Z, Hayasaka H, Kobayashi M, Li W, Guo Z, Jang MH, Kondo A, Choi BI, Iwakura Y, Miyasaka M, Journal of immunology (Baltimore, Md. : 1950), Journal of immunology (Baltimore, Md. : 1950), 182(3), 1287 - 1295, Feb. 2009 , Refereed
  • FRNK expression promotes smooth muscle cell maturation during vascular development and after vascular injury., Sayers RL, Sundberg-Smith LJ, Rojas M, Hayasaka H, Parsons JT, Mack CP, Taylor JM, Arteriosclerosis, thrombosis, and vascular biology, Arteriosclerosis, thrombosis, and vascular biology, 28(12), 2115 - 2122, Dec. 2008 , Refereed
  • Disruption of FRNK expression by gene targeting of the intronic promoter within the focal adhesion kinase gene., Hayasaka H, Martin KH, Hershey ED, Parsons JT, Journal of cellular biochemistry, Journal of cellular biochemistry, 102(4), 947 - 954, Nov. 2007 , Refereed
  • Binding of lymphoid chemokines to collagen IV that accumulates in the basal lamina of high endothelial venules: its implications in lymphocyte trafficking., Yang BG, Tanaka T, Jang MH, Bai Z, Hayasaka H, Miyasaka M, Journal of immunology (Baltimore, Md. : 1950), Journal of immunology (Baltimore, Md. : 1950), 179(7), 4376 - 4382, Oct. 2007 , Refereed
  • Ligand-induced structural changes of the CD44 hyaluronan-binding domain revealed by NMR., Takeda M, Ogino S, Umemoto R, Sakakura M, Kajiwara M, Sugahara KN, Hayasaka H, Miyasaka M, Terasawa H, Shimada I, The Journal of biological chemistry, The Journal of biological chemistry, 281(52), 40089 - 40095, Dec. 2006 , Refereed
  • FRNK, the autonomously expressed C-terminal region of focal adhesion kinase, is uniquely regulated in vascular smooth muscle: analysis of expression in transgenic mice., Hayasaka H, Simon K, Hershey ED, Masumoto KH, Parsons JT, Journal of cellular biochemistry, Journal of cellular biochemistry, 95(6), 1248 - 1263, Aug. 2005 , Refereed
  • Chemokines in tumor progression and metastasis., Tanaka T, Bai Z, Srinoulprasert Y, Yang BG, Hayasaka H, Miyasaka M, Cancer science, Cancer science, 96(6), 317 - 322, Jun. 2005 , Refereed
  • Interaction of neuropeptide Y and Hsp90 through a novel peptide binding region., Ishiwatari-Hayasaka H, Maruya M, Sreedhar AS, Nemoto TK, Csermely P, Yahara I, Biochemistry, Biochemistry, 42(44), 12972 - 12980, Nov. 2003 , Refereed
  • Requirements for signal delivery through CD44: analysis using CD44-Fas chimeric proteins., Ishiwatari-Hayasaka H, Fujimoto T, Osawa T, Hirama T, Toyama-Sorimachi N, Miyasaka M, Journal of immunology (Baltimore, Md. : 1950), Journal of immunology (Baltimore, Md. : 1950), 163(3), 1258 - 1264, Aug. 1999 , Refereed
  • Interactions of Hsp90 with histones and related peptides., Schnaider T, Oikarinen J, Ishiwatari-Hayasaka H, Yahara I, Csermely P, Life sciences, Life sciences, 65(22), 2417 - 2426, 1999 , Refereed
  • Suppression of tumor growth by the 3' untranslated region of mel-18 in 3Y1 cells transformed by the E6 and E7 genes of human papillomavirus type 18., Ishiwatari H, Nakanishi K, Kondoh G, Hayasaka N, Li Q, Yamashita A, Inoue H, Hakura A, Cancer letters, Cancer letters, 117(1), 57 - 65, Jul. 1997 , Refereed
  • Induction of cell death by chimeric L-selectin-Fas receptors., Ishiwatari-Hayasaka H, Kawashima H, Osawa T, Nagata S, Miyasaka M, International immunology, International immunology, 9(4), 627 - 635, Apr. 1997 , Refereed
  • Degradation of p53 only is not sufficient for the growth stimulatory effect of human papillomavirus 16 E6 oncoprotein in human embryonic fibroblasts., Ishiwatari H, Hayasaka N, Inoue H, Yutsudo M, Hakura A, Journal of medical virology, Journal of medical virology, 44(3), 243 - 249, Nov. 1994 , Refereed
  • Correlation between tumorigenicity and expression levels or splicing patterns of transcripts of the human papillomavirus type 16 E6 gene., Inoue T, Kyo S, Kiyono T, Ishibashi M, Ishiwatari H, Hwang YI, Yutsudo M, Hakura A, Japanese journal of cancer research : Gann, Japanese journal of cancer research : Gann, 85(4), 357 - 363, Apr. 1994 , Refereed
  • Isolation of flat revertants from human papillomavirus type 18 E6E7 transformed 3Y1 cells by transfection with a rat embryo fibroblast cDNA expression library., Nakanishi K, Yong-Il H, Ishiwatari H, Takami Y, Hayasaka N, Yutsudo M, Nojima H, Hakura A, Cell structure and function, Cell structure and function, 18(6), 457 - 465, Dec. 1993 , Refereed
  • Acidic regions of cytochrome c1 are essential for ubiquinol-cytochrome c reductase activity in yeast cells lacking the acidic QCR6 protein., Nakai M, Endo T, Hase T, Tanaka Y, Trumpower BL, Ishiwatari H, Asada A, Bogaki M, Matsubara H, Journal of biochemistry, Journal of biochemistry, 114(6), 919 - 925, Dec. 1993 , Refereed

Conference Activities & Talks

  • Functional analysis of the subcapsular sinus floor that allows leukocyte migration from the sinus to the lymph node parenchyma, SASAKI NAOKO, HATA ERINA, UMEMOTO EIJI, HAYASAKA HARUKO, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2014 11 18
  • The role of LPA4/6 receptors in lymphocyte trafficking across high endothelial venules of lymph nodes, HATA ERINA, SASAKI NAOKO, TAKEDA AKIRA, UMEMOTO EIJI, HAYASAKA HARUKO, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2014 11 18
  • The role of LPA receptors on endothelial cells in lymphocyte trafficking across high endothelial venules of lymph nodes, HATA ERINA, SASAKI NAOKO, TAKEDA AKIRA, UMEMOTO EIJI, HAYASAKA HARUKO, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2013 11 18
  • CXCR4 igands promote CCR7-dependent CD4 T cell migration-possible involvement of CCR7 oligomerization, HAYASAKA HARUKO, KOBAYASHI DAICHI, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2013 11 18
  • Migration behavior of naive T cells and activated T cells via afferent lymphatics, HATA ERINA, UMEMOTO EIJI, HAYASAKA HARUKO, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2012 11 12
  • In vivo inhibition of the autotaxin/LPA axis results in inhibition of lymphocyte transmigration across high endothelial venules of lymph nodes, HATA ERINA, BAI ZHONGBIN, CAI LINJUN, UMEMOTO EIJI, HAYASAKA HARUKO, MIYASAKA MASAYUKI, 日本免疫学会総会・学術集会記録,   2011 11 07

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(新学術領域研究(研究領域提案型)), Integrative understanding of biological processes mediated by transient macromolecul ar complexes; New technology for visualizing physiologically metastable states, The role of the management team is to promote and coordinate each project of the Grant-in-Aid for Scientific Research on Innovative Area "Katoteki-Fukugoutai" (transient molecular complex). Specifically, we held the all-member meeting once a year, and the management team meeting on a regular basis. Efforts have been made to spend research funds efficiently by organizing the joint purchase of the reagents, and to promote collaboration between the members. We organized a symposium, and hosted workshops in various domestic conferences. We support the tutorial course for young investigators. We issued newsletters on a regular basis, and wrapped up an annual report at the end of the year.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(新学術領域研究(研究領域提案型)), Analysis of the conformational transitions and functional modifications in membrane proteins, In various cancer cells, the expression of adhesion molecules and chemokine receptor shows a positive correlation with the metastatic potential, suggesting a possible involvement of those molecules in cancer metastasis. CD44 plays a role in cell adhesion by binding to its ligand hyaluronate. Chemokines are critical regulators of cell migration in the context of effective and appropriate immune responses, inflammation, angiogenesis and tumor progression. In this research project, we found that the transition of CD44 molecular structure by ligand binding is possibly involved in the CD44-mediated tumor progression. We also found that the dynamics of chemokine receptor localization on the plasma membrane are important for their function in cell migration.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Understanding of the mechanism underlying the tissue-specific differentiation of high endothelial venule endothelial cells, The high endothelial venules (HEVs) are blood vessels specifically found in lymph nodes and Peyer ’ s patches. Although HEVs express specific chemokines/adhesion molecules which mediate lymphocyte t rafficking across the high-walled endothelial cells (HEV -ECs), the mechanism regulating HEVs’ development and maintenance of the unique properties remain unclear. We performed microarray and real -time quantitative PCR analyses of HEV -ECs and non-HEV -ECs inneonatal mice mesenteric LNs, and identified five transcription factors which are over fifty times more abundantly expressed in developing HEV -ECs than in non-HEV -ECs. By immunohistochemical analysis, we found that one of them showed a restricted expression pattern in the nucleus of ECs of a substantial proportion of blood vessels oflymph nodes from E17.5 to the date of birth, which corresponds temporally to HEV -EC development. The gene knockout mice of this transcription factor showed reduced expression of several HEV -associated genes, implying the functionalcontribution of this gene to HEV -EC differentiation.