- Phylogenetic relationships among accessions in Citrus and related genera based on the insertion polymorphism of the CIRE1 retrotransposon, Akira Horibata, Tsuneo Kato, Open Agriculture, Open Agriculture, 5(1), 243 - 251, Jun. 18 2020 , Refereed
Summary:
AbstractA total of 145 accessions of the genus Citrus and related genera, maintained in the Conservation Garden for Citrus Germplasm at the Experimental Farm of Kindai University, Yuasa, Wakayama, Japan, were examined for their phylogenetic relationships. The present classification was conducted using an inter-retrotransposon amplified polymorphism (IRAP) method based on the insertion polymorphism of a retrotransposon, CIRE1, identified in C. sinensis. The objective of this study was to evaluate the applicability of the IRAP method for citrus classification. The constructed dendrogram showed that the 145 accessions and two outgroup species were successfully classified into five major clades. A large number of C. sinensis accessions were divided into three traditional groups, navel orange, sweet orange, and blood orange, almost corresponding to the sub-clades in the dendrogram. Several other accessions belonging to the same species, and also many hybrid cultivars from crossbreeding, were localized into the respective sub-clades or near positions in the dendrogram. Several unclassified accessions could also be located in the dendrogram, suggesting novel relationships with other accessions. It was concluded that the IRAP method based on CIRE1 insertion polymorphism was suitable for the classification of citrus from a molecular point of view. - Relationship between the Spreading-Stub Phenotype of Rice and Negative Gravitropism of Stems at Tillering Stage, 62, 1 - 5, Nov. 2017 , Refereed
- The horticultural crops production by the plant factories using a translucent solar cell, and the improvement of marketability of the crops using supplementary monochromatic lighting technology., HORIBATA Akira, MATSUMOTO Toshiro, ISES EuroSun 2016 Conference Proceedings, ISES EuroSun 2016 Conference Proceedings, Aug. 2017 , Refereed
- Quantitative trait loci responsible for the difference in gamma-oryzanol content in brown rice between japonica-type and indica-type rice cultivars, Tsuneo Kato, Tetsuya Matsukawa, Akira Horibata, PLANT PRODUCTION SCIENCE, PLANT PRODUCTION SCIENCE, 20(4), 459 - 466, 2017 , Refereed
Summary:gamma-Oryzanol is a main oleophilic component in rice bran oil and has been well recognized as a good dietary supplement for human health, as well as having uses in industrial materials. japonica-type rice cultivars generally showed significantly higher contents of total gamma-oryzanol in brown rice compared with indica-type cultivars, although within-group variation was significant. The objective of this study was to explore quantitative trait loci (QTLs) responsible for the difference in the gamma-oryzanol content between japonica-type and indica-type rice cultivars, using recombinant inbred lines (RILs), backcross inbred lines (BILs), and corresponding chromosome segment substitution lines (CSSLs) derived from crosses between japonica-type and indica-type. Results from RILs and BILs showed that eight QTLs were detected with R-2 from.09 to.16. Nine candidate regions for QTL were also suggested from corresponding CSSLs. These QTLs from RILs and BILs and the candidate regions from CSSLs were not overlapped, although one QTLs was mapped near the boundaries of the respective candidate region. At four QTLs and three candidate regions, alleles or segments from japonica-type caused higher contents than those from indica-type. On the other hand, at the other four QTLs and six candidate regions, alleles or segments from indica-type caused higher contents than those from japonica-type, which is a reverse result to the parental differences. This result strongly suggested that alleles with increasing effects on.-oryzanol content could be accumulated not only from japonica-type but also from indica-type, leading to a potential for increase in gamma-oryzanol content in future breeding programs.
- Environmentally induced un-der-development of seeds in garden pea and its underlying factors, A. Horibata, T. Ito, M. Kotani, T. Kawanishi, H. Shinto, K. Tsuji, S. Kusu, T. Kato, Agricultural Sciences, Agricultural Sciences, 7(7), 439 - 446, Jul. 2016 , Refereed
- Suitable Light Wavelength, Light source and timing of Lighting for Floral Induction in Pea (Pisum saivum L.), 川西孝秀, 小谷真主, 堀端章, 松本比呂起, 楠茂樹, 小谷真主, 堀端章, 松本比呂起, 楠茂樹, Bulletin of the Wakayama Prefectural Experiment Stations of Agriculture, Forestry and Fisheries, Bulletin of the Wakayama Prefectural Experiment Stations of Agriculture, Forestry and Fisheries, 4(4), 41 - 54, Mar. 2016
- Alleles for good grain filling in rice extra-heavy panicle types and their distribution among rice cultivars, Kato, T, A. Horibata, Proceedings of 8th Asian Crop Science Association Conference, Proceedings of 8th Asian Crop Science Association Conference, 51 - 61, Dec. 2015
- 福島における農業復興支援のための太陽光分散利用セル式栽培法の実用化, 鈴木高広, 堀端章, 坂本勝, 古川道郎, 神田紀, 太陽/風力エネルギー講演論文集, 太陽/風力エネルギー講演論文集, 2015, 455‐458 - 458, Nov. 26 2015
- Studies on the transposition activity and utilization of rice transposon mPing, HORIBATA Akira, 1 - 145, Sep. 2015 , Refereed
- A Survey of Environmental Radioactive Cesium Due to the Accident of TEPCO Fukushima Daiichi NPP in Kawamata-machi, Fukushima-ken, YAMANISHI Hirokuni, HORIBATA Akira, SUZUKI Takahiro, FURUKAWA Michio, INAGAKI Masayo, WAKABAYASHI Genichiro, HOHARA Sin-ya, ITOH Tetsuo, TANAKA Naomichi, ISHIWATA Shunji, TAGA Atsushi, OGATA Fumihiko, Journal of Smart Processing, Journal of Smart Processing, 4(6), 268 - 274, 2015
- Dynamics of Starch Content and the Expression Profile of the Genes Involved in Starch Metabolism in Culms of Rice Recombinant Inbred Lines after Heading, Kubo Ryuichi, Horibata Akira, Kato Tsuneo, Journal of crop research, Journal of crop research, (59), 17 - 22, Jul. 2014 , Refereed
Summary:Photoassimilates are accumulated as starch at rice stems before heading, are converted to sucrose, and are translocated to panicles after heading. Efficient translocation of photoassimilates to panicles should be a key to improve poor grain filling of rice cultivars with numerous spikelets per panicle. On the other hand, rice cultivars accumulating photoassimilates at stems should be utilized in the development of forage cultivars as whole crop silage. In this study, we investigated the starch content of the culms on the first node below frag leaf after heading for three years (in 2009, 2010 and 2012), using 9 rice recombinant inbred lines (RILs) (derived from Nakateshinsenbon/Milyang 23) which showed characteristic dynamics of non-structural carbohydrate contents after heading. Among these RILs, we selected three RILs showing stable dynamics in starch contents in culms among different years, and examined the expression of genes involved in starch metabolism in this organ. Results showed that one RIL, which increased the starch content at later stages of grain filling, tended to increase the expression levels of genes for soluble starch synthase (SSIIIB) and sucrose synthase (RSUS1), both of which are involved in starch synthesis pathway. However, other two RILs, which did not show such re-accumulation of the starch content, also showed no increase in the expression levels of these two genes. These results suggested that the expression of these two genes would contribute to the re-accumulation of starch content at the first node culm, and could be available to develop forage rice cultivars.
- Construction of greenhouse for growth promotion of a plant with supplemental LED light using photovoltaic power generated by the see-through solar cells, 松本 俊郎, 堀端 章, 伊藤 卓爾, 太陽/風力エネルギー講演論文集, 太陽/風力エネルギー講演論文集, 2013, 551 - 554, Nov. 2013
- Characterization of a Medicinal Perilla Native to Wakayama Prefecture, and Growth Regulation by Using Monochromatic Lighting, Horibata Akira, Yata Kiyoshi, Matsukawa Tetsuya, Journal of crop research, Journal of crop research, (58), 43 - 50, Jul. 2013 , Refereed
Summary:We found one line of Perilla herb with good smell, as a local genetic resource that may have been cultivated in the Kinokawa plain since ancient times. In this study, firstly, we revealed the morphological and aromatic characteristic of this line by comparison with that of four varieties of Perilla frutescens. Since the leaves of this plant were dark purplish-red color on both surfaces without crepe-like undulations, and this plant contained a notable amount of perillaldehyde and limonene, it was suggested that this line of perilla was one of the varieties belonging to the medicinal red perilla, P. frutescens var. crispa f. purpurea. Secondly, to grow up this resource as a local special product, we have begun to develop the cultivation method for year round production and open up new uses. Supplemental lighting by blue LEDs to the perilla plants which were grown under the natural daylight dreadfully inhibited the flower formation, and allowed the continuous differentiation of young leaves wealthy in perillaldehyde. This cultivation method is useful to inhibit flower formation during short-day period and to improve the functional component content in this perilla herb.
- The utilization of the split solar energy for the horticultural production : Effects of supplementary lighting on the flower formation and the content of functional aromatic substances of medicinal perilla (Perilla frutescens var. crispa f. purpurea), 堀端 章, 伊東 卓爾, 渡辺 俊明, 太陽/風力エネルギー講演論文集, 太陽/風力エネルギー講演論文集, 2012, 393 - 396, Nov. 08 2012
- A novel frameshift mutant allele, fzp-10, affecting the panicle architecture of rice, Tsuneo Kato, Akira Horibata, EUPHYTICA, EUPHYTICA, 184(1), 65 - 72, Mar. 2012 , Refereed
Summary:The rice FRIZZY PANICLE (FZP) locus on chromosome 7, in which an ERF and acidic domain are present, is concerned with the regulation of spikelet meristem identity and the determination of panicle architecture. Many fzp mutants drastically alter panicle morphology with higher-order rachis-branches developing successively instead of the development of floral organs in these mutants. A new mutant showing the same fzp phenotype was induced by gamma-ray irradiation of seeds of a rice cultivar "Gimbozu". Examination of this fzp-like mutant for its allelism to a known allele of fzp-1, nucleotide sequence, and panicle and agronomic characteristics clearly indicated that the allele of this fzp-like mutant is located in FZP. Because there is a previously identified allele called fzp-9, we designated this new allele as fzp-10. fzp-10 has a single nucleotide (cytosine) deletion between the ERF domain and the acidic domain, which results in a frameshift mutation and a premature stop codon, thereby altering the C-terminus of the encoded protein. The degree of higher-order branching in the panicles was significantly reduced in the fzp-10 mutant compared with that of fzp-1. Moreover, the fzp-10 mutant showed highly depressed culm and panicle lengths and panicle number, as well as delayed heading dates compared with its wild type and also with fzp-1. fzp-10 has several new characteristics, its altered nucleotide position and severity of phenotype alteration, and, therefore, could be a new gene resource to examine the function of FZP and the determination of rice panicle architecture.
- Performance of greenhouse for growth promotion of a plant wth supplemental led light using photovoltaic power generated by the see-through solar cells, Toshiro Matsumoto, Akira Horibata, Takuji Itoh, Toshiaki Watanabe, Nobumasa Nitoh, 30th ISES Biennial Solar World Congress 2011, SWC 2011, 30th ISES Biennial Solar World Congress 2011, SWC 2011, 2, 1675 - 1679, Dec. 2011 , Refereed
- The utilization of the splitted solar energy for the horticultural production: effect of night break treatment by red LEDs on the flower formation of a day-neutral plant, garden pea (Pisum sativum), 堀端 章, 小谷 真主, 加藤 恒雄, Proceedings of JSES/JWEA Joint Conference, Proceedings of JSES/JWEA Joint Conference, 2011(0), 333 - 336, Sep. 21 2011
- 〈Original Papers〉The Effect of the Blue LED Supplemental-lighting on Growth and the Content of Functional Chemical Components of the Native Medicinal Perilla (Perilla frutescens), 'Kishu-zairai', 堀端 章, 松川 哲也, 矢田 清, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University, (28), 13 - 20, Sep. 2011 , Refereed
Summary:本研究は日本学術振興会科学研究費補助金22658077および近畿大学生物理工学部戦略的研究No.09-IV-1, 2010の助成を受けた.[要旨] 本研究は、機能性成分含有量の高い'紀州在来薬用紫蘇' の自然光併用型植物工場での高効率生産を目的とする。自然光下(ガラスハウス内)で栽培している'紀州在来薬用紫蘇'および'赤ちりめんしそ'に青色LEDの光を24時間連続照射し、草丈、主茎節数および機能性成分の一種であるペリルアルデヒドの含有量の変化を調査した。青色光補光による草丈の減少と主茎節数の増加が観察されたが、これらの変化は同時に観察された青色光補光による花成阻害に起因すると考えられた。一方、収穫された葉におけるペリルアルデヒド含有量は青色光照射によって有意に増加した。 [Abstract] Two cultivars of perilla, 'Kishu-zairai' (Perilla frutescens var. crispa f. purpurea, red s mooth 1 eat) and 'Aka-chirimen-shiso' (Perilla frutescens var. crispa f. crispa, red wrinkled leaf), were grown in a greenhouse and were lighted continuously by blue LEDs (460nm, approximately 10μmol/m^2/s). As a result, it was revealed that the blue supplemental-lighting reduced the plant height and increased the number of nodes on main stem in both kind of perilla. These two phenomena were thought to be associated with the inhibition of flower formation by the blue supplemental-lighting. While, the content of perillaldehyde (a kind of functional chemical component) in harvested leaves was increased by the blue supplemental-lighting.
- 青色LEDによる補光が’紀州在来薬用紫蘇’の生育および機能性成分含有量に及ぼす作用, 堀端章, 堀端章, 松川哲也, 矢田清, Mem Fac Biol Oriented Sci Technol Kinki Univ, Mem Fac Biol Oriented Sci Technol Kinki Univ, (28), 13-20 - 20, Sep. 2011 , Refereed
- The utilization of the splitted solar energy for the horticultural production: the effects of the monochromatic lighting on the productivity of pea, 堀端 章, 小谷 真主, 加藤 恒雄, Proceedings of JSES/JWEA Joint Conference, Proceedings of JSES/JWEA Joint Conference, 2010(0), 509 - 512, Nov. 04 2010
- 太陽光栽培条件下の赤色/青色光補光による花の経済性の向上, HORIBATA AKIRA, ITO TAKUJI, NITO NOBUMASA, WATANABE TOSHIAKI, MATSUMOTO TOSHIRO, 太陽/風力エネルギー講演論文集, 太陽/風力エネルギー講演論文集, 2009, 563 - 564, Nov. 05 2009
- The effect of complementary UV irradiation on the flower color of spray-type chrysanthemum cultivated under the solar light, HORIBATA Akira, ITOH Takuji, NITO Nobumasa, WATANABE Toshiaki, MATSUMOTO Toshiro, Proceedings of JSES/JWEA Joint Conference, Proceedings of JSES/JWEA Joint Conference, 2008(0), 103 - 106, Nov. 06 2008
- Formation of a new plant by means of supplemental irradiation consisted of See-through solar cells, MATSUMOTO Toshiro, WATANABE Toshiaki, ITOH Takuji, HORIBATA Akira, Proceedings of JSES/JWEA Joint Conference, Proceedings of JSES/JWEA Joint Conference, 2007(0), 109 - 111, Oct. 25 2007
- A Database System for High-Throughput Transposon Display Analyses of Rice, INOUE Etsuko, YOSHIHIRO Takuya, KAWAJI Hideya, HORIBATA Akira, NAKAGAWA Masaru, Journal of Japan Society of Information and Knowledge, Journal of Japan Society of Information and Knowledge, 16(1), 28 - 38, Jan. 2006 , Refereed
Summary:We developed a database system to support whole experiments which analyze correlation between genotypes and phenotypes by using transposons. We also evaluated the system by applying to our experiments with transposon mPing in rice. Our research group grow large-scale mutant series of rice by taking advantage of mPings, and study correlation between genotypes and phenotypes for predictions of gene functions. However, the analytical phase, in which we find mutation spots from waveform data, involves several problems from a viewpoint of labor amount, and it becomes one of bottlenecks in large-...
- The Effects of the Selective Bases on Discrimination in Transposon Display with Anchored PCR (APCR) in Rice (Oryza saliva L.), Matsui Kazuyuki, Horibata Akira, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 16(0), 7 - 12, Sep. 2005 , Refereed
Summary:We are currently performing a functional genomics of rice (Oryza sativa L.) via insertion mutagenesis induced by mPing, an active inherent transposon of rice. Transposon display is an excellent method for exhaustive detection of numerous polymorphisms at mPing insertion sites in whole rice genome. In many cases, however, too many copies of mPing were present originally in rice genome, which makes difficult to identify truly polymorphic signals from such background involving many non-polymorphic amplified signals. In this study, we verified whether one or two selective bases added to an adap...
- High Efficient Use of Solar Cell Power for Supplemental Fluorescent Blue Light in Green House, Watanabe Toshiaki, Matsumoto Toshiroh, Itoh Takuji, Horibata Akira, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 16(0), 53 - 57, Sep. 2005 , Refereed
Summary:A mixture of artificial and natural light is indispensable for producing crops. This study develops a low-cost system for the short-time irradiation of plants in addition to natural daylight. Blue light (400-500nm) is employed by a highly autonomous production system. Using the proposed method, the total consumed energy is reduced by about 26%. The assistant light is supplied in the early morning to avoid energy generation conflicts.
- A Design of Shape Memory Alloy Microgripper and Characteristic Evaluation of the Prototype, Mizutani Katsumi, Omasa Mitsushi, Horibata Akira, Tokugawa Yusuke, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 16(0), 67 - 76, Sep. 2005 , Refereed
Summary:This study describes a prototype TiNi shape memory alloy microgripper which could be used in an assembly process of a micro factory. The microgripper consists of two units, displacement direction-conversion unit and unit for the displacement drive, integrated with same material for miniaturization. Assuming to grip a pin of the about 0.5mm diameter, measuring the characteristics of the alloy on deformation and transformation, designing a microgripper using FEM, trial manufacturing and the characteristic evaluation of the gripper were carried out. As the results, the following were obtained:...
- Effect of the growth stage for cold-acclimation treatment on inducing cold-acclimation tolerance of rice（ Oryza sativa L..） seedling, 前田 哲, 堀端 章, Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 10,13-18(10), 13 - 17, Mar. 2005 , Refereed
Summary:筆者らは、水稲の幼苗期耐冷性を、幼苗が急激に強度の低温に曝されたときに即座に発現する常発型耐冷性と、幼苗が徐々に強い低温にさらされていく過程でその発現が誘導される順化型耐冷性の2 つの側面に分けて考えている。本報では、この順化型耐冷性が、これを誘導する低温順化処理を行う幼苗のステージによって影響をうけるか否かを調査した。その結果、催芽種子の播種後3日から12日の幼苗に低温順化処理を行った場合、順化型耐冷性の発現が安定的に認められることが示された。
- Effect of the Short-time Irradiation of Blue Light in the Dawn on the Growth of Pea Plants (Pisum sativum L.), Horibata Akira, Itoh Takuji, Watanabe Toshiaki, Matsumoto Toshiroh, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, Memoirs of the School of Biology-Oriented Science and Technology of Kinki University, 14,17-22(0), 17 - 22, Sep. 2004 , Refereed
Summary:To construct the technical foundation of the new cropping system using short-time irradiation of blue light in addition to the natural daylight, we designed the irradiation equipment that consisted of blue fluorescence light and translucent solar cell. The additional irradiation of blue light to plantlets of pea (Pisum sativum L. cultivar Kisyu-Usui) for a half hour before daylight influenced the growth of the pea plants. The plant height, number of nodes on the main stem and fresh weight of the pea plants were significantly increased with the increase of relative intensity of irradiated bl...
- The effect of slender-glume gene slg on the methylation of genomic DNA., 堀端 章, Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 0(9), 27 - 33, Feb. 2004 , Refereed
Summary:イネ細粒遺伝子slg の内部に見出されたトランスポゾンmPing の転移活性は、slg 座の遺伝子型によって制御されているように見えた。本研究では、その原因を明らかにするため、slg がゲノム全体のシトシンのメチル化に及ぼす影響を調査した。その結果、slg は、ゲノム全体のシトシンのメチル化にも、また、CGメチラーゼおよびCNG メチラーゼの活性にも影響しないことが明らかになった。
- Genetic and morphogenetic analysis of two mutants for panicle neck formation of rice (Oryza sativa L.), Horibata Akira, Memoirs of The School of Biology-Oriented Science and Technology of Kinki University, Memoirs of The School of Biology-Oriented Science and Technology of Kinki University, 12(0), 15 - 21, Sep. 2003 , Refereed
Summary:The gene concerned with the formation of panicle neck of the rice plant is the gene which works at the conversion from the vegetative growth stage to the reproductive growth stage, therefore, the general and molecular genetic analysis of such genes is very important for breeding of rice (Oryza sativa L.). In this study, first of all, genetic analysis was done about two different types of mutant for knob formation on panicle neck, which are induced from a rice variety Gimbozu by gamma irradiation. As the result, it was clarified that this trait in both cases was controlled by single recessive gene. Next, the morphological observation of the knob on panicle neck was carried out using scanning electron microscopy. The result showed that this knob would be derived from hypertrophy of the palea leaf base and the panicle neck knot to support developing palea leaves. The gene related to this trait may regulate the timing of the growth termination of palea leaves.
- Induction of cold tolerance by cold treatment to the seedling in a japonica rice and retaining term of the induced tolerance., 前田 哲, 堀端 章, Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 8,15-20(8), 15 - 20, Nov. 2002 , Refereed
Summary:水稲の幼苗期耐冷性には、幼苗が急激に強度の低温に遭遇すると即座に発現する常発型耐冷性と幼苗が徐々に強い低温にさらされていく過程で発現が誘導される順化型耐冷性の2 種が存在すると考えている。本報では、このうち順化型耐冷性に着目し、品種銀坊主の幼苗に休止期間を挟む2 回の低温処理、低温順化処理と耐冷性評価処理を行い、耐冷性の発現における低温順化処理の効果と誘導された順化型耐冷性の保持期間について検討した。その結果、5℃の低温順化処理によって順化型耐冷性が誘導されて耐冷性評価処理の障害を軽減すること、また、その誘導された順化型耐冷性は少なくとも8日間保持されることが明らかになった。
- Variation of cold tolerance at seedling stage in the F3 progeny from a cross between japonica rice varieties, Gimbozu*Hayayuki., KAKIKUBO YOSHIHIRO, HORIBATA AKIRA, 近畿大学先端技術総合研究所紀要, 近畿大学先端技術総合研究所紀要, (8), 21 - 26, Nov. 2002 , Refereed
- Mutations induced by the mutator on the fatty acid composition of 8 lipid molecular classes extracted from rice（Oryza sativa L.）leaves., 堀端 章, 山縣 弘忠, Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 7,18-28(7), 18 - 28, Jan. 2002 , Refereed
Summary:水稲品種銀坊主種子のγ 線照射後代に見いだされた突然変異誘発遺伝子mutator を効率良く育種に利用するには，mutator によって誘発される変異のスペクトラムを明らかにする必要がある．本研究では，この観点から，葉から抽出した脂質の脂肪酸組成に関する変異の検出を試みた．種子に含まれる全脂質の脂肪酸組成に変異が認められたmutator 由来の突然変異固定型系統12系統について，葉から抽出した全脂質を８脂質分子種に分画した上で，各脂質分子種における脂肪酸組成を調査したところ，いずれの分子種についても，脂肪酸含有比率が原品種銀坊主と明瞭に異なる系統が認められ，mutator によって脂肪酸組成に関する変異が誘発されることが示された．
- Reversion Mutability of a Slender-glume Gene slg and its Influence on the Activity of a Mutagenic Factor in Rice, Horibata Akira, Yamagata Hirotada, Breeding research, Breeding research, 2(3), 125 - 132, Sep. 2000 , Refereed
Summary:A mutagenic factor, mutator, that was detected in the progeny from a γ-ray irradiated M_1 seed of a japonica rice variety Gimbozu, generated novel mutations at many different loci, when activated by the reversion of a mutable slender-glume gene slg, which was induced by γrays in the above identical M_1 seed. For the effective utilization of the mutator in plant breeding, the reversion mutability of slg was analyzed for its genetic behavior and relationship with the mutator activity. First, changes in the reverse mutation frequency of slg(RMF)were traced among several generations, using 39 slender-glume lines which originated from a single slender-glume mutant but were more or less different from each other in background genotype. The results revealed that the mutability of slg was essentially an inherited character, but that it often increased or decreased. Secondly, eleven of the 39 slender-glume lines were each estimated for the mutator activity by examining the frequency of mutator-induced heading-date mutations that appeared in the porgenies of non-slender-glume plants from the slender-glume lines, using 424 sublines derived from the 11 lines. As a result, it was found that the mutability of slg did not influence the mutagenic activity of the mutator.
- Mutations induced on lipid metabolic pathway by the mutator in rice (Oryza sativa L..), 堀端 章, 前田 哲, 山縣 弘忠, Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University, Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University, 4(4), 29 - 38, Mar. 2000 , Refereed
Summary:継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University水稲品種銀坊主種子のγ線照射後代に見いだされた突然変異誘発造伝子mutatorは、上記γ線照射で誘発された1細粒遺伝子slgの正常粒遺伝子(＋)への復帰突然変異に伴って活性化し、形態的あるいは生理的形質に種々の変異を誘発する。しかし、植物体構成物質の成分についてはまだ突然変異誘発の有無が明らかにされていない。そこで、本研究では、生体膜を形成する重要な成分であり、すでに一部の代謝反応系が解明されている脂質に着目し、mutatorによって誘発された形態的あるいは生理的形質に関する突然変異固定型48系統を供試して、脂質代謝経路におけるmutator誘発突然変異の有無を探索した。まず、供試全系統について全脂質の脂肪酸組成を調査したところ、組成脂肪酸の含有比率が正規分布から有意に外れる9系統が見いだされた。ついで、これら9系統のうち解析に十分な量の種子が得られた6系統および上記9系統についで大きな変異を示した8系統計14系統について、種子中に多く含まれる3脂質分子種フォスファチジルセリン、フォスファチジルエタノールアミンおよびフォスファチジルコリンの脂肪酸組成を調査したところ、いずれの分子種についても、脂肪酸含有比率が原品種銀坊主と有意に異なる系統が相当数認められた、このことから,mutatorは植物体構成物質の成分に関する変異も誘発すると考えられた。 （英文） The mutator that was found out first in the M_3 progeny derived from a γ-ray irradiated M_1 seed of a japonica rice variety Gimbozu, causes novel mutations in many different loci for morphological and/or physiological traits, when activated by the reversion of a mutable slender-glume gene slg., which was induced by γ rays in the above identical M_1 seed. However, it has not yet been confirmed if the mutator also mutate the structure material of plants. Lipid is one of the most important components of biomembrane, and has partly been elucidated for metabolic pathway. In view of this situation, the mutations that might have been induced by the mutator on lipid metabolic pathway was screened through the analysis of the fatty acid composition of lipid contained in seeds, using 48 mutant lines with some mutator-induced morphological and/or physiological mutations. First, the 48 lines were analyzed for their respective fatty acid composition in total lipid, and nine of these lines each proved to be significantly apart from the population mean in at least one of the percent contents of 5 sorts of fatty acid. Then, 14 lines the percent contents of which largely deviated from the population mean, inclusive of 6 from the above 9 lines, were selected and analyzed for fatty acid composition of 3 lipid molecular classes, phosphatidylserine, phosphatidylethanolamine and phosphatidylcholine. As a result, it was observed in all the classes that a considerable number of lines were significantly different from the original variety in the percent content of fatty acid. This may strongly suggest that the mutator induces mutations also in the components of structure material of plants.
- An early leaf-senescence mutant induced by tissue culture in rice (Oryza sativa L.), Horibata Akira, Maruyama Seigou, Yamagata Hirotada, Memoirs of The School of Biology-Oriented Science and Technology of Kinki University, Memoirs of The School of Biology-Oriented Science and Technology of Kinki University, 6(6), 23 - 30, Feb. 2000 , Refereed
Summary:The genetics of leaf senescence, one of the most important yield-determinant characters in cereals, has been a great concern in plant breeding. From this point of view, an early leaf-senescence (ELS) mutant induced by tissue culture of a rice variety, Nipponbare, and detected first in the next generation (R_1) of a regenerated plant (R_o) was analyzed for the gene concerning the relevant mutation. First, the R_2 progeny lines derived from the normal-type plants which belonged to the same R_1 line as the ELS mutant did were examined for their genetic segregation with early leaf-senescence. The result revealed that ELS was controlled by a single recessive gene. Secondly, using 10 normal-type plants and 15 mutants of R_3 eneration, chlorophyll content in several leaves per plant was measured every 3 days for 2 months around heading time. The result suggested that the gene responsible for ELS might advance the time of beginning of senescence in lower leaves (8-12 th leaves), while retard the progress of senescence in each leaf.
- Relationship of Cold Tolerance between Young Seedling and Cultured Tissue in Rice (Oryza sativa L.)., HORIBATA AKIRA, HAYASHI KOICHI, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, 44(44), 17 - 20, Mar. 31 1999
- Aptitude for Direct Sowing as Inferred from the Responses to Low Temperature of Seed and Seedling in Rice (Oryza sativa L.)., HORIBATA AKIRA, MAEDA SATORU, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, 44(44), 53 - 56, Mar. 1999 , Refereed
- Relationship of the Mutagenic Activity of the Mutator with the Mutability of Slender-glume Gene slg in Rice., HORIBATA AKIRA, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, 43,16-21(43), 16 - 21, Mar. 1998 , Refereed
- A Mutable Gene for Seed Sterility that Occurred in a Rice Line, IM52., HORIBATA AKIRA, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, (41), 33 - 35, Mar. 1996 , Refereed
- Adaptability to Tissue Culture of the Slender-glume Rice Lines Carrying a Mutator., HORIBATA AKIRA, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, (40), 41 - 46, Mar. 1995
- Method for early developmental stage detection of non-slender-glume plants induced by the mutator of rice., OKUMOTO YUTAKA, HORIBATA AKIRA, SATO AYAKO, TANISAKA TAKATOSHI, YAMAGATA HIROTADA, 近畿作物・育種研究, 近畿作物・育種研究, 35,75-78(35), 75 - 78, Mar. 1990
- LKF, the locus regulating large grains in the rice cultivar ‘Fusayoshi’, is identical to the loci encoding a serine/threonine protein phosphatase with Kelch motif, Tsuneo Kato, Akira Horibata, Euphytica, Euphytica, 214(3), 60, Mar. 01 2018 , Refereed
Summary:The LKF locus, which regulates grain size in the rice cultivar ‘Fusayoshi’ showing large grain, has been mapped to the proximal part of the long arm of chromosome 3. An incomplete dominant allele, Lkf, caused large grain size of Fusayoshi. The structure and function of this locus, however, have not yet been determined. In a similar position to LKF on chromosome 3, two loci, Os03g0407400 (GS3) and LOC_Os03g44500, have been already reported as loci also regulating rice grain size. The objective of the present study was to determine the nucleotide sequences of both Os03g0407400 and LOC_Os03g44500 for different alleles at the LKF locus. Results showed that only one known single nucleotide polymorphism (SNP) in exon 10 of LOC_Os03g44500 was detected between a large-grain allele (Lkf) and a small-grain allele at the LKF locus, whereas no polymorphisms in Os03g0407400. This SNP, visualized using a dCAPS marker, clearly demonstrated nearly complete co-segregation with grain length in an F2 population segregating the Lkf at LKF. Other large-grain mutant lines with large-grain alleles at the LKF locus, which originated from another cultivar ‘Gimbozu’, also showed the same SNP in exon 10 of LOC_Os03g44500. It was concluded from these results that LKF is identical to LOC_Os03g44500, and the detected SNP in exon 10, at least, which is included in Kelch-like repeat motif, could be essential for expression of the large-grain phenotype.
- Distorted genetic segregation of the transposon mPing at the long arm of chromosome 12 in rice, Akira Horibata, Yoshihiro Kakikubo, Tsuneo Kato, BREEDING SCIENCE, BREEDING SCIENCE, 65(4), 340 - 344, Sep. 2015 , Refereed
Summary:A class II transposable element, mPing exists in the rice genome ubiquitously and can transpose even in ordinary cultivation conditions. A copy of mPing was identified at the long arm of chromosome 12. In reciprocal backcrossed F(1)s between a heterozygote and a homozygote without mPing, the male gametes with this mPing from heterozygotes were transmitted to the next generation at a lower frequency than those without mPing, resulting in distorted genetic segregation in self-fertilized progenies, as well as in F(1)s after backcrossing. Pollens with mPing tended to germinate on stigma less than those without mPing. These results, however, could not explain the lower transmission of male gametes with mPing. In addition, no excision of mPing was observed in a homozygote. Thus, it was suggested that male gametes with mPing were eliminated partly from pollination to fertilization by negative competition against male gametes without mPing. Less formation of microspores with mPing in meiosis could also be a cause for the distorted segregation, although this could not be examined. At least two ORFs, whose functions have not been identified, are located near this mPing. It is plausible that either of these ORFs or both are necessary for the normal functioning of male gametes.
- Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha), Hirotomo Kihara, Maya Tanaka, Katsuyuki T. Yamato, Akira Horibata, Atsushi Yamada, Sayaka Kita, Kimitsune Ishizaki, Masataka Kajikawa, Hideya Fukuzawa, Takayuki Kohchi, Yoshihiko Akakabe, Kenji Matsui, PHYTOCHEMISTRY, PHYTOCHEMISTRY, 107, 42 - 49, Nov. 2014 , Refereed
Summary:Eight-carbon (C8) volatiles, such as 1-octen-3-ol, octan-3-one, and octan-3-ol, are ubiquitously found among fungi and bryophytes. In this study, it was found that the thalli of the common liverwort Marchantia polymorpha, a model plant species, emitted high amounts of C8 volatiles mainly consisting of (R)-1-octen-3-ol and octan-3-one upon mechanical wounding. The induction of emission took place within 40 min. In intact thalli, 1-octen-3-yl acetate was the predominant C8 volatile while tissue disruption resulted in conversion of the acetate to 1-octen-3-ol. This conversion was carried out by an esterase showing stereospecificity to (R)-1-octen-3-yl acetate. From the transgenic line of M. polymorpha (des6(KO)) lacking arachidonic acid and eicosapentaenoic acid, formation of C8 volatiles was only minimally observed, which indicated that arachidonic and/or eicosapentaenoic acids were essential to form C8 volatiles in M. polymorpha. When des6(KO) thalli were exposed to the vapor of 1-octen-3-ol, they absorbed the alcohol and converted it into 1-octen-3-yl acetate and octan-3-one. Therefore, this implied that 1-octen-3-ol was the primary C8 product formed from arachidonic acid, and further metabolism involving acetylation and oxidoreduction occurred to diversify the C8 products. Octan-3-one was only minimally formed from completely disrupted thalli, while it was formed as the most abundant product in partially disrupted thalli. Therefore, it is assumed that the remaining intact tissues were involved in the conversion of 1-octen-3-ol to octan-3-one in the partially disrupted thalli. The conversion was partly promoted by addition of NAD(P)H into the completely disrupted tissues, suggesting an NAD(P)H-dependent oxidoreductase was involved in the conversion. (C) 2014 Elsevier Ltd. All rights reserved.
- A Novel Cropping Method for Production of High Functioning Crops by Utilizing On-site Solar Energy, Akira Horibata, Tetsuya Matsukawa, Takuji Itoh, Toshiaki Watanabe, Toshiroh Matsumoto, 2013 ISES SOLAR WORLD CONGRESS, 2013 ISES SOLAR WORLD CONGRESS, 57, 1502 - 1507, 2014 , Refereed
Summary:The utilization efficiency of solar energy through photosynthesis by crops is only a few percent at most. Therefore, most solar energy in the field (on-site) remains unused. Plants are, however, able to use certain types of monochromatic light as an environmental signal, and they exhibit various physiological responses to even small amounts of the light. In particular, the significant induction of secondary metabolite production by blue light is well documented. Here, we propose a novel cropping method where unused solar energy is captured on-site by solar cells and converted to monochromatic light by using LEDs. The crops are then irradiated to stimulate enhanced production of secondary metabolites, thus improving crop function without the need for an external energy source. In this study, the effect of blue light on the production of functional secondary metabolites was evaluated in perilla (Perilla frutescens var. crispa f. purpurea), a traditional medicinal crop in Japan. Perilla leaves contain several functional substances such as perillaldehyde, rosmarinic acid, and anthocyanins, which have notable stomachic, antipyretic, detoxification, sedation, and diuretic efficacy. Plants were grown under natural daylight supplemented with blue light irradiation (460 nm, approx. 10 mu mol/m(2)/s, 24 h,) for 108 days before harvest. Control plants were grown without supplemental lighting. The content ratio of perillaldehyde in plants grown with blue light was 60% higher at harvest than in the controls. Moreover, in the vegetative growth stage, the perillaldehyde content increased significantly after only three days of blue light irradiation. These findings suggest that the present approach will benefit agricultural practices in developing countries through more efficient usage of on-site solar energy. (C) 2014 The Authors. Published by Elsevier Ltd.
- Loss-of-function of a ubiquitin-related modifier promotes the mobilization of the active MITE mPing, Takuji Tsukiyama, Shota Teramoto, Kanako Yasuda, Akira Horibata, Nanako Mori, Yutaka Okumoto, Masayoshi Teraishi, Hiroki Saito, Takatoshi Tanisaka, GENES & GENETIC SYSTEMS, GENES & GENETIC SYSTEMS, 88(6), 358 - 358, Dec. 2013 , Refereed
- Loss-of-function of a ubiquitin-related modifier promotes the mobilization of the active MITE mPing, Takuji Tsukiyama, Shota Teramoto, Kanako Yasuda, Akira Horibata, Nanako Mori, Yutaka Okumoto, Masayoshi Teraishi, Hiroki Saito, Akiko Onishi, Kanako Tamura, Takatoshi Tanisaka, Molecular Plant, Molecular Plant, 6(3), 790 - 801, 2013 , Refereed
Summary:Miniature inverted-repeat transposable elements (MITEs) are widespread in both prokaryotic and eukaryotic genomes, where their copy numbers can attain several thousands. Little is known, however, about the genetic factor(s) affecting their transpositions. Here, we show that disruption of a gene encoding ubiquitin-like protein markedly enhances the transposition activity of a MITE mPing in intact rice plants without any exogenous stresses. We found that the transposition activity of mPing is far higher in the lines harboring a non-functional allele at the Rurm1 (Rice ubiquitin-related modifier-1) locus than in the wild-type line. Although the alteration of cytosine methylation pattern triggers the activation of transposable elements under exogenous stress conditions, the methylation degrees in the whole genome, the mPing-body region, and the mPing-flanking regions of the non-functional Rurm1 line were unchanged. This study provides experimental evidence for one of the models of genome shock theory that genetic accidents within cells enhance the transposition activities of transposable elements.© 2012 The Author.
- Non-random distribution of the alleles for good grain filling at OsAGPS2 and OsSUT1 among a wide range of rice (Oryza sativa L.) cultivars, Tsuneo Kato, Akira Horibata, BREEDING SCIENCE, BREEDING SCIENCE, 61(2), 217 - 220, Jun. 2011 , Refereed
Summary:Rice (Oryza sativa L.) genes AS2-2 and SUT1-2, which are alleles at OsAGPS2 (AS2) on chromosome 8 and OsSUT1 (SUT1) on chromosome 3, respectively, were suggested to improve grain filling in extra-heavy panicle rice cultivars. This study examined the distribution of these alleles among 315 rice cultivars. A discriminant function based on phenol reaction of hulls, apiculus hair length and KClO3 sensitivity in seedlings was used to classify cultivars into japonica (192 cultivars) and indica (123 cultivars). Genotyping for the AS2 and SUT1 alleles were performed with In/Del and SSR markers, respectively. In japonica cultivars, the frequencies of AS2-2 and SUT1-2 were very low (3.6% and 6.3%, respectively), whereas they were more common in indica cultivars (56.1% and 74.0%, respectively). In addition, in indica cultivars the genotypes of AS2-2 SUT1-2, as well as AS2-1 SUT1-1, were more frequent than expected from independent segregation of these alleles. It was suggested that artificial and/or natural selection contributed to this non-random distribution of alleles at AS2 and SUT1. The alleles for good grain filling should, thus, be explored in indica cultivars to improve the grain filling of extra-heavy panicle types of rice in future breeding programs.
- Effects of the Alleles at OsAGPS2 and OsSUT1 on the Grain Filling in Extra-Heavy Panicle Type of Rice, Tsuneo Kato, Ayano Taniguchi, Akira Horibata, CROP SCIENCE, CROP SCIENCE, 50(6), 2448 - 2456, Nov. 2010 , Refereed
Summary:Rice (Oryza sativa L.) cultivars of extra-heavy panicle type (EHPT), which have numerous spikelets in a panicle, often fail to realize their high-yield potential due to poor grain filling in general, while some EHPTs showed relatively better grain filling. This study first surveyed nucleotide polymorphisms in rice genes possibly related to grain filling, that is, genes for ADPglucose pyrophosphorylase (AGPase) subunits, OsAGPS2 and OsAGPL2, and a sucrose transporter gene, OsSUT1, to clarify the causes underlying such genetic variation. Many types of polymorphisms were detected in all three genes, but none of them affected amino acid sequences. OsAGPS2 and OsSUT1 had an identical series of polymorphisms in two EHPT cultivars, Milyang 23 and Nanjing 11, both of which have a high degree of grain filling, while another series for these genes was detected in other EHPTs showing a low grain filling. The former polymorphisms could constitute alleles for good grain filling in OsAGPS2 and OsSUT1, designated as AS2-2 and SUT1-2, respectively. Second, new molecular markers based on the polymorphisms for OsAGPS2 and OsSUT1 were developed. Finally, three EHPT cultivars with AS2-2 SUT1-2 genotype showed a higher degree of grain filling and higher activity of AGPase than three other EHPTs with another genotype at these loci. These results suggest that these AS2-2 and SUT1-2 alleles can contribute to the improvement of poor grain filling of EHPTs assisted with the developed markers.
- A transposon, Ping, is integrated into intron 4 of the DROOPING LEAF gene of rice, weakly reducing its expression and causing a mild drooping leaf phenotype, Yoshihiro Ohmori, Mafumi Abiko, Akira Horibata, Hiro-Yuki Hirano, PLANT AND CELL PHYSIOLOGY, PLANT AND CELL PHYSIOLOGY, 49(8), 1176 - 1184, Aug. 2008 , Refereed
Summary:The YABBY gene DROOPING LEAF (DL) regulates midrib formation in the leaves and carpel specification in the flowers of rice (Oryza sativa L). We found a new dl allele (dl-5) that caused a mild phenotype in the descendants of a mutable line, IM294. In plants homozygous for this allele, midrib structures were formed but their sizes were reduced. Molecular analysis revealed that a transposon, Ping, was inserted in the fourth intron of DL. Together with mPing and Pong, Ping is a member of a transposon family that was first identified as a group of active transposable elements in rice. Our finding of the Ping insertion in the DL gene is a first indication that Ping is active in planta, and that it can be transposed and integrated in a new locus. Ping seems to be still active because it was excised from intron 4 of DL at a relatively high frequency in rice calli. Real-time PCR analysis and in situ hybridization indicated that DL transcript levels were reduced in dl-5 without alterations in the spatial expression pattern of the DL gene. The reduction of DL expression may be due to inefficient splicing of the large intron caused by Ping insertion. By comparing the expression levels of DL and leaf phenotypes in the dl mutants with different severities, we confirmed our previous hypothesis that DL promotes cell proliferation in the central region of leaf primordia, and that this cell proliferation is critical for midrib formation in the mature leaves.
- Mobilization of a transposon in the rice genome, T Nakazaki, Y Okumoto, A Horibata, S Yamahira, M Teraishi, H Nishida, H Inoue, T Tanisaka, NATURE, NATURE, 421(6919), 170 - 172, Jan. 2003 , Refereed
Summary:Rice (Oryza sativa L.) is an important crop worldwide and, with the availability of the draft sequence(1,2), a useful model for analysing the genome structure of grasses(3),(4). To practice efficient rice breeding through genetic engineering techniques, it is important to identify the economically important genes in this crop. The use of mobile transposons as gene tags in intact plants is a powerful tool for functional analysis because transposon insertions often inactivate genes(5). Here we identify an active rice transposon named miniature Ping (mPing) through analysis of the mutability of a slender mutation of the glume(6)-the seed structure that encloses and determines the shape of the grain. The mPing transposon is inserted in the slender glume (slg) mutant allele but not in the wild-type allele. Search of the O. sativa variety Nipponbare genome identified 34 sequences with high nucleotide similarity to mPing, indicating that mPing constitutes a family of transposon elements. Excision of mPing from slg plants results in reversion to a wild-type phenotype. The mobility of the transposon mPing in intact rice plants represents a useful alternative tool for the functional analysis of rice genes.
