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KARUBE Zin’ichi


FacultyDepartment of Biotechnology and Chemistry
DegreePh.D. in Science
Commentator Guide
Last Updated :2020/09/30

Education and Career

Academic & Professional Experience

  •   2018 04 ,  - 現在, Faculty of Engineering, Department of Biotechnology and Chemistry , Kindai University
  •   2014 05 ,  - 2018 03 , Center for Water Environment Studies, Ibaraki University

Research Activities

Research Areas

  • Environmental science/Agricultural science, Environmental dynamics
  • Life sciences, Ecology and environmental science
  • Environmental science/Agricultural science, Environmental effects of chemicals
  • Environmental science/Agricultural science, Environmental effects of radiation

Published Papers

  • Repeatability and reproducibility of measurements of low dissolved radiocesium concentrations in freshwater using different pre-concentration methods, Momo Kurihara, Tetsuo Yasutaka, Tatsuo Aono, Nobuo Ashikawa, Hiroyuki Ebina, Takeshi Iijima, Kei Ishimaru, Ramon Kanai, Zin'ichi Karube, Yae Konnai, Tomijiro Kubota, Yuji Maehara, Takeshi Maeyama, Yusuke Okizawa, Hiroaki Ota, Shigeyoshi Otosaka, Aya Sakaguchi, Hisaya Tagomori, Keisuke Taniguchi, Masatoshi Tomita, Hirofumi Tsukada, Seiji Hayashi, Sangyoon Lee, Susumu Miyazu, Moono Shin, Takahiro Nakanishi, Tatsuhiro Nishikiori, Yuichi Onda, Takuro Shinano, Hideki Tsuji, JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 322(2), 477 - 485, Nov. 2019 , Refereed
    Summary:We assessed the repeatability and reproducibility of methods for determining low dissolved radiocesium concentrations in freshwater in Fukushima. Twenty-one laboratories pre-concentrated three of 10 L samples by five different pre-concentration methods (Prussian-blue-impregnated filter cartridges, coprecipitation with ammonium phosphomolybdate, evaporation, solid-phase extraction disks, and ion-exchange resin columns), and activity of radiocesium was measured. The z-scores for all of the Cs-137 results were within +/- 2, indicating that the methods were a good degree of precision. The relative standard deviations (RSDs) indicating the reproducibility among different laboratories were larger than the RSDs indicating the repeatability in each separate laboratory.
  • Novel method for low level Sr-90 activity detection in seawater by combining oxalate precipitation and chelating resin extraction, Hirofumi Tazoe, Hajime Obata, Masatoshi Tomita, Shinya Namura, Jun Nishioka, Takeyasu Yamagata, Zin'ichi Karube, Masatoshi Yamada, GEOCHEMICAL JOURNAL, GEOCHEMICAL JOURNAL, 51(2), 193 - 197, 2017 , Refereed
    Summary:Sr-90 released into seawater from nuclear bomb testing and nuclear facility accident sites requires long-term monitoring. However, measuring Sr-90 in seawater is still difficult and time-consuming. This study is aimed at improving the preconcentration method for successive purification using DGA Resin chromatography, which is applied to Sr-90 analysis in seawater. At natural pH, the oxalate coprecipitation technique effectively collected Sr (84% in Sr yield) from seawater without Mg or Na. For Sr-90 determination, Y-90 in secular equilibrium with Sr-90 was directly extracted using Fe coprecipitation and DGA Resin chromatography. The proposed method simplifies the analytical processes associated with the conventional method. Analytical results for surface seawater in the western North Pacific (0.81 +/- 0.06 Bq m(-3), n = 5) were consistent with those obtained using the conventional method (0.81 +/- 0.07 Bq m(-3), n = 5).
  • Radiostrontium monitoring of bivalves from the Pacific coast of eastern Japan, Zin'ichi Karube, Yoko Inuzuka, Atsushi Tanaka, Katsuaki Kurishima, Nobuharu Kihou, Yasuyuki Shibata, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 23(17), 17095 - 17104, Sep. 2016 , Refereed
    Summary:In early April 2011, radiostrontium was accidentally released from the Fukushima Daiichi Nuclear Power Plant to the Pacific coast of eastern Japan. We developed a simple procedure to analyze radiostrontium levels in marine mussels (Septifer virgatus) and seawater using crown ether (Sr Resin; Eichrom). Then, we used our method to describe the spatial and temporal distribution of radiostrontium in mussels and seawater on the Pacific coast of eastern Japan from 2011 to 2013 and for 2015. Activity of Sr-90 in mussels and seawater decreased with distance from the Fukushima Daiichi Nuclear Power Plant and between 2011 and 2013 tended to be higher in areas south of the Fukushima Daiichi Nuclear Power Plant than to the north of it. Activity in mussels and seawater also tended to decrease from 2011 to 2013 and by 2015 had reached levels experienced prior to the Fukushima accident. Our results suggest that radiostrontium discharged from the Fukushima Daiichi Nuclear Power Plant was dispersed by coastal currents in a southerly direction along the Pacific coast of eastern Japan from 2011 to 2013, following which its activity decreased to background levels by 2015.
  • Determination of strontium-90 from direct separation of yttrium-90 by solid phase extraction using DGA Resin for seawater monitoring, Hirofumi Tazoe, Hajime Obata, Takeyasu Yamagata, Zin'ichi Karube, Hisao Nagai, Masatoshi Yamada, TALANTA, TALANTA, 152, 219 - 227, May 2016 , Refereed
    Summary:It is important for public safety to monitor strontium-90 in aquatic environments in the vicinity of nuclear related facilities. Strontium-90 concentrations in seawater exceeding the background level have been observed in accidents of nuclear facilities. However, the analytical procedure for measuring strontium-90 in seawater is highly demanding. Here we show a simple and high throughput analytical technique for the determination of strontium-90 in seawater samples using a direct yttrium-90 separation. The DGA Resin is used to determine the abundance of strontium-90 by detecting yttrium-90 decay (beta-emission) in secular equilibrium. The DGA Resin can selectively collect yttrium-90 and remove naturally occurring radionuclides such as K-40, (210)pb, Bi-214, U-238, and Th-232 and anthropogenic radionuclides such as Ba-140 and La-140. Through a sample separation procedure, a high chemical yield of yttrium-90 was achieved at 95.5 +/- 2.3%. The result of IAEA-443 certified seawater analysis (107.7 +/- 3.4 mBq kg(-1)) was in good agreement with the certified value (110 +/- 5 mBq kg(-1)). By developed method, we can finish analyzing 8 samples per day after achieving secular equilibrium, which is a reasonably fast throughput in actual seawater monitoring. By processing 3 L of seawater sample and applying a counting time of 20 h, minimum detectable activity can be as low as 1.5 mBq kg(-1), which could be applied to monitoring for the contaminated marine environment. Reproducibility was found to be 3.4% according to 10 independent analyses of natural seawater samples from the vicinity of the Fukushima Daiichi Nuclear Power Plant in September 2013. (C) 2016 Elsevier B.V. All rights reserved.
  • Three decades of environmental specimen banking at the National Institute for Environmental Studies, Japan, Zin-ichi Karube, Atsushi Tanaka, Akinori Takeuchi, Yoshikatsu Takazawa, Mai Takagi, Ayako Kinoshita, Haruhiko Seyama, Yasuyuki Shibata, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 22(3), 1587 - 1596, Feb. 2015 , Refereed
    Summary:After two decades operation of the initial environmental specimen banking, a new program, Environmental Time Capsule Program, started in 2002 as a government-supported long-term program to construct a firm scientific basis for various environmental research studies. The program consists of long-term environmental specimen banking activity and specimen collection of endangered wildlife and is based on cryogenic sample preservation facility called Environmental Time Capsule building, which completed construction in 2004. After 9 years of extensive research, research focuses have been selected and the program was reorganized to the environmental sample collection part and endangered wildlife collection part in 2011. Due to huge environmental disaster caused by the Great East Japan earthquake and the tsunami as well as subsequent nuclear power plant accident at Fukushima, a new sampling and monitoring program started at affected areas in collaboration with the reorganized environmental sample collection and archiving program. Outlines of the quality assurance and quality control (QA/QC) activities in the program and future perspective under related international activities, particularly Stockholm Convention, are reported.
  • Seasonal and site-specific variability in terrigenous particulate organic carbon concentration in near-shore waters of Lake Biwa, Japan, Yoichiro Sakai, Zin'ichi Karube, Tomohiro Takeyama, Ayato Kohzu, Chikage Yoshimizu, Toshi Nagata, Ichiro Tayasu, Noboru Okuda, LIMNOLOGY, LIMNOLOGY, 14(2), 167 - 177, Apr. 2013 , Refereed
    Summary:Identifying sources of particulate organic matter (POM) is important for clarifying fundamental mechanisms by which lake food webs are sustained. We determined carbon and nitrogen stable isotope ratios of POM in near-shore waters of Lake Biwa, a large, meso-eutrophic lake in Japan, to estimate relative contributions of terrigenous particulate organic carbon (T-POC), plankton-derived POC (P-POC) and epilithon-derived POC (E-POC) to POC in near-shore waters. Samples were collected during different months (November, February, May and July) at 29 sites located near the mouth of tributary rivers with different discharge and catchment land use. The data revealed that POC mainly consisted of P-POC and T-POC, with relative contributions varying widely over season and among locations. E-POC generally contributed little to the near-shore POC. Path analyses revealed that the concentration of riverine POC whose isotopic signatures were similar to those of rice straws increased with a larger %paddy field area in the catchment of tributary rivers, which subsequently enhanced T-POC inputs to near-shore waters through riverine transportation. Furthermore, our results suggested that T-POC contribution was influenced, with a time lag, by wave-driven turbulence and shore topography, which appear to affect sedimentation and resuspension of T-POC.
  • Sulfur stable isotope signature identifies the source of reduced sulfur in benthic communities in macrophyte zones of Lake Biwa, Japan, Zin'ichi Karube, Naoki Okada, Ichiro Tayasu, LIMNOLOGY, LIMNOLOGY, 13(3), 269 - 280, Dec. 2012 , Refereed
    Summary:Carbon, nitrogen and sulfur stable isotope ratios (delta C-13, delta N-15 and delta S-34) were measured from water column sulfate, sediments, particulate organic matter (POM), macrophytes, periphyton, macroinvertebrates and fish, sampled from the littoral, open water and macrophyte zones of Lake Biwa. In most of the littoral zones, the delta C-13 and delta N-15 values of organisms indicated that POM and periphyton support the consumers. However, in the dysoxic interior macrophyte (IM) zone, the delta C-13 values of Sinotaia quadrata histrica, Propsilocerus akamusi and Anodonta woodiana were lower than that of all resources. The delta N-15 values of S. quadrata histrica were lower than those of P. akamusi and A. woodiana. delta C-13 and delta N-15 values thus failed to distinguish the foods of these consumers. The delta S-34 values of sediment and S. quadrata histrica were lower than those of water column sulfate, suggesting that this consumer incorporated reduced sulfur derived from sulfate reduction in the sediment by ingesting detritus. In contrast, the delta S-34 values of P. akamusi and A. woodiana were higher than that of S. quadrata histrica, suggesting that they incorporated sulfur derived from water column sulfate by ingesting POM. Consequently, delta C-13, delta N-15 and delta S-34 signatures provide complementary estimates of foods for consumers in this freshwater lake.
  • Physical structure of habitat network differently affects migration patterns of native and invasive fishes in Lake Biwa and its tributary lagoons: stable isotope approach, Jun-ya Shibata, Zin'ichi Karube, Mamiko Oishi, Mana Yamaguchi, Yukiko Goda, Noboru Okuda, POPULATION ECOLOGY, POPULATION ECOLOGY, 53(1), 143 - 153, Jan. 2011 , Refereed
    Summary:A good understanding of how migratory animals use their habitat network is expected to provide important insights for the prediction of population dynamics at both local and regional scales. We focused on how the physical structure of a habitat network could affect fish migration between Lake Biwa and its tributary lagoons. Although the lagoons provide suitable breeding and nursery grounds for native fishes, it is a matter of concern that they can also be a hotbed of invasive exotic fishes. Here, we assessed the migration patterns of native crucian carps (Carassius spp.) and exotic largemouth bass (Micropterus salmoides) and bluegill sunfish (Lepomis macrochirus) using their carbon and nitrogen stable isotopes as migration tracers. As there were marked differences in the carbon isotope ratios of basal food webs between the main lake and its tributary lagoons, stable isotopic signatures of individual fishes collected from each lagoon enabled us to judge whether they were residents of the lagoon or recent immigrants from the main lake. The analysis revealed that native and invasive fishes showed different migration patterns across a variety of lagoons. Exotic fishes frequently immigrated from the main lake to the lagoon as the distance of the channel connecting these two habitats was short. For native crucian carps, in contrast, their migrations were unaffected by the channel distance but were promoted by narrow channels. Physical barriers of weirs and dense vegetation within the channel obstructed their migrations. Such ecological information on migration behavior will be vital to plan designs for habitat restoration to conserve native fishes.
  • Carbon and nitrogen stable isotope ratios of macroinvertebrates in the littoral zone of Lake Biwa as indicators of anthropogenic activities in the watershed, Zin'ichi Karube, Yoichiro Sakai, Tomohiro Takeyama, Noboru Okuda, Ayato Kohzu, Chikage Yoshimizu, Toshi Nagata, Ichiro Tayasu, ECOLOGICAL RESEARCH, ECOLOGICAL RESEARCH, 25(4), 847 - 855, Jul. 2010 , Refereed
    Summary:Carbon and nitrogen stable isotope ratios (delta(13)C and delta(15)N) of macroinvertebrates inhabiting littoral zones of lakes can serve as useful indicators of material loading from the watershed. We collected snails (Semisulcospira spp.) and bivalves (Unio douglasiae biwae Kobelt) from 29 littoral sites in Lake Biwa near the mouths of river tributaries with various human population density (HPD) and land-use patterns. The delta(13)C and delta(15)N signatures were determined for three potential food sources: particulate organic matter in the pelagic zone (PPOM), riverine particulate organic matter from tributaries (RPOM) and epilithic organic matter in the littoral zone (EOM). The stable isotope mixing model revealed that snails relied mainly on EOM, and bivalves on PPOM and RPOM. Multiple regression analysis showed that intersite variation in delta(15)N for snails was best explained by HPD, while variation in delta(15)N of EOM and nitrate was explained to a lesser extent by HPD. Comparison with isotope signatures of their food sources and riverine nutrients revealed that snails assimilated anthropogenic nitrogen from wastewater in the watershed. Our results also showed that the delta(13)C value of bivalves was marginally related to the fraction of paddy fields in the watersheds. In conclusion, the isotope signatures of macroinvertebrates inhabiting the littoral zone can be useful indicators of anthropogenic impacts from the watershed.
  • Trophic polymorphism in bluegill sunfish (Lepomis macrochirus) introduced into Lake Biwa: Evidence from stable isotope analysis, Kimiko Uchii, Noboru Okuda, Ryuji Yonekura, Zin-Ichi Karube, Kazuaki Matsui, Zen-Ichiro Kawabata, Limnology, Limnology, 8(1), 59 - 63, Apr. 2007 , Refereed
    Summary:Trophic polymorphism was recently reported in introduced bluegill (Lepomis macrochirus) in Lake Biwa, Japan, where three morphs are specialized in benthic invertebrates (benthivorous type), submerged aquatic plants (herbivorous type), and zooplankton (planktivorous type). We evaluated the long-term effects of food resource utilization by these trophic morphs using stable isotope ratios, δ15N and δ13C. A significant difference in δ15N was found between the benthivorous and planktivorous types. The planktivorous type had the higher δ15N value, which corresponded with the value expected from its prey, zooplankton. The lower δ15N value of the benthivorous type would be derived from the lower δ15N values of benthic prey organisms compared to zooplankton. These results support previous findings that the benthivorous and planktivorous types have different food resource utilization. In contrast, the δ15N and δ13C values of the herbivorous type were distinctly different from the expected values, indicating that this type was unlikely to utilize aquatic plants substantially, contradicting the results of the dietary analysis. © The Japanese Society of Limnology 2007.


  • 活魚測定法を用いた渓流魚の給餌飼育における放射性セシウムの取り込み・排出のモニタリングと魚体内のセシウム低減化法に関する研究, 鈴木貴大, 中里亮治, 上田仁, 苅部甚一, 日本水環境学会年会講演集, 52nd, 443,   2018 03 09 ,
  • 避難指示区域内の山地河川に生息する渓流魚の放射性セシウム濃度の推移について, 中里亮治, 鈴木貴大, 上田仁, 川上拓磨, PARK Soeun, 上野山諒一, 櫛井優志, 苅部甚一, 鈴木仁根, 加藤健一, 日本水環境学会年会講演集, 52nd, 442,   2018 03 09 ,
  • 山地河川における福島第一原子力発電所事故由来の放射性ストロンチウムの分布調査, 苅部甚一, 鈴木貴大, 川上拓磨, PARK Soeun, 上田仁, 中里亮治, 鈴木仁根, 加藤健一, 田副博文, 田中敦, 日本水環境学会年会講演集, 52nd, 439,   2018 03 09 ,
  • 避難指示区域内の渓流域における福島第一原子力発電所事故由来の放射性ストロンチウムの分布, 苅部甚一, 鈴木貴大, 川上拓磨, PARK Soeun, 上田仁, 中里亮治, 鈴木仁根, 加藤健一, 田副博文, 田中敦, 日本水環境学会年会講演集, 51st, 269,   2017 03 09 ,
  • 渓流生態系における放射性セシウムの移行メカニズムに関する研究(その1):渓流魚ならびに水生昆虫の放射性Cs濃度について, 鈴木貴大, 川上拓磨, PARK Soeun, 上田仁, 苅部甚一, 中里亮治, 鈴木仁根, 加藤健一, 日本水環境学会年会講演集, 51st, 266,   2017 03 09 ,
  • 渓流生態系における放射性セシウムの移行メカニズムに関する研究(その2):渓流魚の標識放流およびインサイト実験について, 中里亮治, 鈴木貴大, 川上拓磨, PARK Soeun, 上田仁, 苅部甚一, 鈴木仁根, 加藤健一, 日本水環境学会年会講演集, 51st, 267,   2017 03 09 ,
  • 環境DNAで外来種アメリカナマズを追う, 本郷真理, 垣見直希, 芝田直樹, 加納光樹, 苅部甚一, 平山拓弥, 高尾大貴, 山中裕樹, 日本生態学会大会講演要旨(Web), 64th, ROMBUNNO.P1‐O‐413 (WEB ONLY),   2017 ,
  • DGAレジンを用いた環境中放射性ストロンチウムの分析, 田副博文, 山形武靖, 苅部甚一, 山田正俊, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.P‐141,   2016 06 06 ,
  • 福島第一原子力発電所事故に由来する放射性ストロンチウムの東日本太平洋沿岸におけるモニタリング―事故から4年後の状況―, 苅部甚一, 苅部甚一, 犬塚洋子, 田中敦, 栗島克明, 木方展治, 柴田康行, 環境化学討論会要旨集(CD-ROM), 25th, ROMBUNNO.1E‐05,   2016 06 06 ,
  • 内湾生態系における放射性核種の挙動と影響評価に関する研究 平成24~26年度, 堀口敏宏, 苅部甚一, 牧秀明, 荒巻能史, 児玉圭太, 国立環境研究所研究プロジェクト報告 SR(Web), 111, WEB ONLY,   2016 01 29 ,
  • The Distribution of Radioactive Strontium in Coastal Area of Fukushima Prefecture, Japan, 苅部 甚一, 化學工業, 64, 7, 512, 515,   2013 07 ,
  • Horizontal and vertical distributions of radiocesium in the sediment of Lake Kasumigaura, Tanaka Atsushi, Karube Zin'ichi, Kohzu Ayato, Imai Akio, Hayashi Seiji, Abstracts of Annual Meeting of the Geochemical Society of Japan, 60, 0,   2013 , 10.14862/geochemproc.,
  • Simplification of radioactive strontium analysis method, Karube Zin'ichi, Tanaka Atsushi, Shibata Yasuyuki, Abstracts of Annual Meeting of the Geochemical Society of Japan, 60, 0,   2013 , 10.14862/geochemproc.,
    Summary:福島第一原子力発電所事故に由来する放射性ストロンチウムの環境汚染実態の解明はセシウムに比べて遅れている.その原因として,分析法の難しさもあると思われる.そこで本研究では,以前から行われている放射性ストロンチウム分析法(文部科学省 2003)を元に,新たにクラウンエーテル処理等を用いることで,その分析法の簡略化を検討した.その結果,検討した手法を放射性ストロンチウム分析に組み入れることで,従来法に比べて使用する試薬の種類と量,処理工程の数を削減できる可能性があることが分かった.今後は,放射性核種分離の観点からの評価が必要である.
  • Analysis of radioactive strontium dynamics in Fukushima coastal area, Karube Zin'ichi, Tanaka Atsushi, Kurishima Katsuaki, Kihou Nobuharu, Shibata Yasuyuki, Abstracts of Annual Meeting of the Geochemical Society of Japan, 59, 0,   2012 , 10.14862/geochemproc.,
    Summary:福島県沿岸の放射性ストロンチウムの分布と挙動を明らかにするために、相馬市(原発から北に37km)、南相馬市(同30km)およびいわき市(原発から南に50km)沿岸で調査を行った。二枚貝の放射性ストロンチウム(Sr-90)分析の結果、相馬市沿岸:0.03 ±0.01Bq/kg、南相馬市沿岸:0.04 ±0.01Bq/kg、いわき市沿岸:0.06±0.01Bq/kgであり、同時に分析した放射性セシウムの結果と同様に原発南側で放射能が高くなる傾向が示唆された。