KINDAI UNIVERSITY


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SHIRAISHI Kohei

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FacultyDepartment of Biotechnology and Chemistry / Graduate School of System Enginnering / Research Institute of Fundamental Technology for Next Generation
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/484-shiraishi-kouhei.html
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Last Updated :2020/09/30

Education and Career

Education

  •  - 1986 , Osaka City University, Graduate School of Engineering

Academic & Professional Experience

  •   2009 04 ,  - 現在, Research Institute of Fundamental Technology for Next Generation, Kindai University
  •   1994 01 ,  - 現在, Professor, Major in Systems Engineering, Graduate School of Systems Engineering
  •   1994 01 ,  - 現在, Kindai University
  •   1987 ,  - 現在, Faculty of Engineering, Department of Biotechnology and Chemistry , Kindai University
  •   1998 08 ,  - 1999 07 , Post Doctual Fellow, Organic Chemistry and Polymer Chemisitury Institute, Heinrich-Heine Dusseldorf University

Research Activities

Research Areas

  • Life sciences, Medical systems
  • Life sciences, Biomaterials
  • Nanotechnology/Materials, Structural and functional materials
  • Nanotechnology/Materials, Polymer chemistry

Research Interests

  • Functional material, Polymer synthesis, Biomaterial

Published Papers

  • Preparation of a UCST type of thermos-responsive sulfobetaine polymer modified poly(ethylene terephthalate)films by Ar plasma-post polymerization method and recovery of attached HeLa cells on the film by heating with laser irradiation, Risa Katayama, Musashi Ikeda, Kohei Shiraishi, Akikazu Matsumoto, Chie Kojima, Langmuir, Langmuir, 35(37), 12229 - 12235, Sep. 2019 , Refereed
  • Immobilization of Thermoresponsive Poly(N-isoporopylacrylamide) on Glass Substrate by Surface-Initiated Atom Transfer Radical Polymerization and Thermal Stimuli-Exfoliation of Human Immortal Mesenchymal Stem Cells, Tatsuki Nousou, Akinori Imajo, Kohei Shiraishi, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 72(6), 354 - 360, 2015 , Refereed
    Summary:Thermoresponsive polymer patterned-immobilized cell microarrays can be used as stem cell diagnosis and non-invasive recovery devices. In order to develop such an array, we grafted poly(N-isopropylacrylamide) (PNiPAAm) with controlled graft-PNiPAAm chain length and density on a glass substrate by the activator regenerated by electron transfer (ARGET)/atom transfer radical polymerization (ATRP) method. The grafting density was controlled by the concentration of 2-bromoisobutylbromide as a reagent on amino-functionalized glass. The grafted PNiPAAm chain length could also be controlled by the polymerization time. Cell attachment and thermal stimuli-exfoliation of immortal mesenchymal stem cells (hiMSCs) as a model stem cell was examined on such surfaces. The amount of hiMSCs attached decreases by increasing grafted PNiPAAm chain length. The thermal stimuli-exfoliation of attached hiMSC by cooling to 15 degrees C for 30 min exceeded 80%. The recovery of a hiMSC sheet by the thermal stimuli-exfoliation was observed on the PNiPAAm-g-glass with a PNiPAAm layer thickness of 19.3 nm.
  • Low Temperature Plasma Irradiation of a PNiPAAm-Grafted O-2 Enrichment Membrane and Its Effect on Cell Proliferation, Tatsuki Nousou, Akinori Imajo, Kohei Shiraishi, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 72(11), 667 - 672, 2015 , Refereed
    Summary:In order to use a poly(-phenyl-2-(trimethylsilyl)acetylene) [PTMSDPA] O-2 enrichment membrane as a cell culture substrate, it was irradiated with an Ar plasma and grafted with poly(N-isopropylacrylamide) [PNiPAAm]. The average amount of grafted PNiPAAm was 1.4 0.7 X 10-2 mg cm(-2), and the degree of graft PNiPAAm was 29 +/- 13. The contact angle (9) of water at 25 C after treatment decreased from 9 = 90 for the untreated PTMSDPA to theta= 48 degrees. HeLa cells attached and proliferated on the grafted polymer (g-PTMSDPA) at 37 degrees C, above the lower critical solution temperature of g-PTMSDPA. After the cultivation of HeLa cells, the thermal stimuli -exfoliation was also examined at 20 degrees C. The ratio of number of thermal stimuli -exfoliation cells to the total number of attached HeLa cells of 3.3 0.3 X 104 cells cm(-2) on g-PTMSDPA was about 28 0.9%, while little thermal stimuli -exfoliation was observed in the case of untreated PTMSDPA. The effect of 02 -supply on cell proliferation was also evaluated by supplying O-2 from the back side of the g-PTMSDPA film at 0.01 MPa, with a flow rate of 20 sccm, for 24h. The number of HeLa cells and SK-N-SH cells growth under O-2-supply were about 1.4 and 1.3 times higher than that without O-2-supply, respectively.
  • Preparation of UCST types of thermoresponsive sulfobetaine polymer modified poly(ethylene terephthalate) films by Ar plasma-post polymerization method, SHIRAISHI Kohei, Research Reports of the Faculty of Engineering, Kinki University, Research Reports of the Faculty of Engineering, Kinki University, 48, 19 - 24, Dec. 2014
  • Preparation of poly[(meth)acrylamide)] having L-lysine moiety and its effect on fibrinolytic activity, Kohei Shiraishi, Sinko Wakisaka, Junji Satozaki, Kazuo Sugiyama, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 69(1), 39 - 46, Jan. 2012
    Summary:In order to develop a novel biomaterial showing antithrombogenisity, we prepared zwitterionic poly [N-α-acrylamide-L-lysine] [poly (α-LysAA)] or poly[N-α-methacrylamide-L-lysine] [poly-(α- LysMA)] with a selective binding activity to fibrinolytic proteins [plasminogen (Plg), tissue-type plasminogen activator (t-PA)]. Surface Plasmon Resonance (SPR) measurements on poly(α-LysAA) immobilized sensor surfaces found that Plg and t-PA were strongly bound to poly(α-LysAA) for Plg with 3.89 × 10 -7 M -1 for t-PA with 1.55 × 10 -7 M -1 as a binding constant, respectively, while other serum proteins such as albumin, γ-globulin, and fibrinogen were weakly bound. The competitive adsorption of albumin and Plg on the immobilized polymer was also carried out. A specific interaction was found between poly(±-LysAA) and Plg. The maximam rate constant for the hydrolysis of chromogenic plasmin substrate (S-2251) by Plg/t-PA in the presence of poly(α-LysAA) was found to be V max = 20.0 × 10 -3 mM · min -1. The enhanced enzymatic reaction of Plg/t-PA and S-2251 was also observed in the presence of poly(α-LysAA) or poly(α-LysMA). © 2012, The Society of Polymer Science, Japan.
  • Blood Compatibility of Amphiphilic Poly(N-a-acrylamide-L-lysine-b-dimethylsioxane)Block Copolymers, SHIRAISHI Kohei, Journal of Biomaterials and Nanobiotechnology, Journal of Biomaterials and Nanobiotechnology, 2, 337 - 346, 2011
  • Development of aHigh Mechanical Strength Poly(L-lactic acid) Composite Reinfoced by Natural Rubber, 47(4), 131 - 137, 2010
  • Blood compatibility of self-assembiled poly (N-α-methacrylamide-L-lysine-b-dimethylsiloxane) copolymers, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 65(2), 150 - 156, Feb. 2008 , Refereed
    Summary:Block copolymers, poly (LysMA-b-DMS)-1-3 with different molecular weight, were prepared by the conversion of poly[N-α-methacrylamide-N-ε-t-butoxycarbonyl-L-lysin-t-butyl ester (BLMA)-b-dimethylsiloxane (DMS)] which were obtained from the radical polymerrization of BLMA initiated with an azo-initiator having poly-dimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly(LysMA-b-DMS) showed microphase-separated structures. The self-organization of poly(LysMA-b-DMS) was analyzed by fluorescence and 1H NMR measurements. The critical micelle concentrations (CMC) were = 500-2000 ing L-1. The micelle had diameters from 221 to 274 nm. The blood compatibility of poly (LysMA-b-DMS) was evaluated from the maximum velocity of hydrolysis (Vmax) for the enzymatic reaction of thrombin and a synthetic substrate S-2238 in the presence of poly(LysMA-b-DMS). Vmax was 1.82 × 10-2 - 1.95 × 10-2 mM min-1. The maximum rate of conversion of plasminogen to plasmin by tissue plasminogen activator (t-PA) in the presence of poly(LysMA-b-DMS) using S-2251 was faster than the case of absence of poly(LysMA-b-DMS).
  • Evaluation of surface characteristic of poly(ethylene terephthalate) film modified with thermoresponsible polymers using bacterial random peptide library method, Kohei Shiraishi, Tetsuyuki Shigesada, Kazuhiko Yamamoto, Kazuo Sugiyama, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 65(2), 132 - 139, Feb. 2008 , Refereed
    Summary:In order to develop a more precise evaluation method for the application as a cell scaffold, the surface of thermoresponsible poly (N-isopropylacrylamide) (PNiPAAm) grafted PET (g-PET) was characterized by a bacterial random peptide library method (FliTrx™: Invitrogen). The g-PET was prepared by an Ar plasma-post polymerization method. From the analysis of selective 12 amino acids sequences, we could observe that the surface of g-PET was hydrophilic at 25°C and hydrophobic surface at 37°C, respectively. In addition, the Kyte & Doolittle parameter of g-PET was - 67.6 on hydrophilic surface at 25°C and - 28.4 for the hydrophobic surface at 37°C. On the other hand, the Kyte & Doolittle parameter of the untreated PET was 34.3 at 25°C and 42.2 at 37°C, respectively. The Selection of amino acids sequences changed drastically on the g-PET by raising the panning temperature from 25°C to 37°C.
  • Molecular assembly and blood compatibility of poly[2-(methacryloyloxy)ethyl phosphorylcholine-b-dimethylsiloxane] block copolymers, Nobuyuki Tanigawa, Kobei Shiraishi, Kazuo Sugiyama, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 64(6), 373 - 379, Jun. 2007 , Refereed
    Summary:Block copolymers, poly (MPC-b-DMS)-I∼IV, were prepared by the radical polymerization of 2-(methacryloyloxy) ethyl phosphorylcholine (MPC) initiated with an azo-initiator having polydimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly (MPC-b-DMS) was found to form microphase- separated structures. The self-organization of poly(MPC-b-DMS) was analyzed by fluorescence and 1H NMR measurements. The critical micelle concentrations of poly (MPC-b-DMS) were 90-600 mg L-1. The blood compatibility of poly(MPC-b-DMS)-I∼IV was evaluated from the Michaelis constant (Km) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238. Km was found to be 0.10-0.16 mM.
  • Preparation of thermo-sensitive polymer-grafted poly (ethyleneterephthalate) films by Ar plasma irradation-post polymerization technique and thermal stimuli-exfoliation of HeLa cells on their films, Kohei Shiraishi, Megumi Ohdan, Kazuma Maeda, Kazuo Sugiyama, Katsuyuki Suzuki, Hiroshi Hosoya, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 63(9), 613 - 620, Sep. 2006 , Refereed
    Summary:In order to develop a novel cell cultured material capable of thermal stimulating cell detachment from its surface, we studied the biocompatible and thermosensitive copolymer consisting of N-(2-hydroxypropyl) methacrylamide (HPMA) and methyl methacrylate (MMA) [poly(HPMA-co-MMA): HPMA/MMA = 100/27.6 (molar ratio)]. With N-isopropylacylamide (NiPAAm) as a control of thermosensitive polymer, the copolymers were grafted onto poly (ethyleneterephthalate) (PET) by Ar plasma irradiation-post polymerization technique. From the contact angle (θ) measurements to water at 25°C, the θ values of poly (HPMA-co-MMA) grafted PET (g-PET-I: amount of grafted polymer = 0.125 mg cm-2) and poly (NiPAAm) grafted PET (g-PET-II: 0.109 mg cm-2) decreased from θ=80° (the original PET) to θ=52° and θ=50°, respectively. HeLa cells attached and proliferated on g-PET-I and g-PET-II at 37°C, above the lower critical solution temperature of poly (HPMA-co-MMA). After the cultivation of HeLa cells, the thermal stimuli-exfoliation was also examined at 4°C for 2 h. The ratios of the number of thermal stimuli-exfoliation cells to the total number of attached cells on g-PET-I or g-PET-II were 73% and 45%, respectively, while little thermal stimuli-exfoliation was observed in the case of untreated PET.
  • Laser Sintering of Stainless Steel Using Resin Powder, Solid Freeform Fabrication Proceedings(CD-ROM), Solid Freeform Fabrication Proceedings(CD-ROM), May 2006
  • Characterization of thermo-responsive poly [N-(2-hydroxypropyl)methacrylamide - Dimethylsiloxane] block copolymers, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama, Zairyo/Journal of the Society of Materials Science, Japan, Zairyo/Journal of the Society of Materials Science, Japan, 55(4), 391 - 396, Apr. 2006 , Refereed
    Summary:Block copolymers, Poly (HPMA-b-DMS)-1-7, were obtained from polymerization of N-(2-hydroxypropyl) methacrylamide (HPMA) initiated with polydimethylsiloxane (PDMS) type of azo-initiator [4,4-azobis (polydimethylsiloxane)-4-cyanopentanoate (azo-PDMS)]. From XPS measurements of freeze dried samples, the hydrophilic PHPMA segment was found to migrate to the surface of Poly (HPMA-b-DMS) films in water. Poly (HPMA-b-DMS) showed lower contact angle (θ= 54°-100°) than PDMS itself (θ= 103°). It was also found that the contact angle of block copolymers was changed in response to change in temperature, i.e., θ= 64°-79° at 40°C and θ= 54°-69° at 20°C for Poly (HPMA-b-DMS). The amount of adsorption of albumin on the Poly (HPMA-b-DMS) film was depressed at 20°C compared to the case at 40°C. For the purpose of an application to a thermo-responsive drug delivery system, copolymer hydrogel Gel (HPMA-b-DMS) was also prepared by radical copolymerization of HPMA and N, N'-ethylenebisacrylamide initiated with azo-PDMS.
  • Analysis of pH response for amphoteric Poly(O-methacryloyl-L-serine) and interaction with serum protein by fluorescence spectroscopy, Kohei Shiraishi, Koji Miura, Go Asami, Masushi Kohta, Kazuo Sugiyama, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 60(1), 30 - 37, 2003 , Refereed
    Summary:The amphoteric polymer having L-serine residues in the side chain as a pH responsible hydrogel component was prepared as follows: L-serine with protected amino and carboxyl groups, N -tert-butoxycarbonyl-O -methacryloyl-L-serine diphenylmethyl ester (Boc-SerMA-Ph2) and 2-(dansyloxy) ethyl methacrylate (DnsEMA) were copolymerized by a radical mechanism with 2,2' -dimethyl-azobisisobutyrate in tetrahydrofuran at 60 °C for 20 h. Deprotection of the resulting polymer was carried out in 80% trifluoroacetic acid aqueous solution to give amphoteric poly(SerMA labeled with a dansyl group [poly(SerMA-co-DnsEMA)]. The fluorescence spectral changes of a dansyl-labeled poly(SerMA) solution at pH ranging from 1.2 to 12.0 revealed that poly(SerMA) shows pH response. The interaction between a serum protein such as bovine serum albumin (Alb), human γ-globulin (Glo), or bovine serum fibrinogen (Fib), and poly(SerMA-co-DnsEMA) was also examined by means of fluorescence spectroscopy in a phosphate buffered solution at pH 5.7 for Alb and Fib, pH 6.2 for Glo. The interaction between Alb and poly(SerMA-co-DnsEMA) was observed, whereas no definite interaction was confirmed for Glo and Fib.
  • Preparation and characterization of polyetherurethaneureas containing methyl- or fluoro substituted biphenyldiyl in hard segments, K Sugiyama, S Akita, Y Tomoi, K Hanaki, K Shiraishi, K Ueda, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (2), 139 - 146, Feb. 1997
    Summary:Polyetherurethaneureas (PEUUs) including methyl- or fluoro substituted biphenyldiyls(BP, nMBP, nFBP) in main chain were obtained from a typical two step addition polymerization of polytetrahydrofuran #1000(PTHF) to 4,4'-methylene bis(phenyl isocyanate) (MPI) in the presence of the substituted biphenyldiols, using ethylenediamine (EDA) as a chain extension reagent. Biphenyldiols used were 4,4'-biphenyldiol (BP), 3,3'-dimethyl-4,4'-biphenyldiol (2MBP), 3,3', 5,5'-tetramethyl-4,4'-biphenyldiol(4MBP), 3,3'-difluoro-4,4'-biphenyldiol (2MBP), 3,3', 5,5'-tetrafluoro-4,4'-biphenyldiol (4FBP), and 2,2', 3,3', 5,5', 6,6'-octafluoro-4,4'-biphenyldiol (8FBP). Polyaddtion with a molar ratio of 0.5 : 0.5 : 2 : 1 for the biphenyidiol : PTHF : MPI : EDA in the mixed solvent of DMSO and IBMK(1 : 1) gave the PEUUs such as PEUU-BP, PEUU-nMBP, PEUU-nFBP. Parent polyetherurethaneurea (PEUU) was also prepared with a molar ratio of 1 : 2 : 1 for PTHF : MPI : EDA. XPS spectra of the PEUUs indicated that the hydrophobic segments containing the substituted biphenyldiyl moieties are located on the surface of the PEUUs film in air. The measurements of contact angle to water confirmed that the introduction of methyl groups or fluorine atoms into biphenyl ring results in higher hydrophobicity of PEUUs film surface. The tensile modulus(E) showed the values of E=109.1 MPa and E=129.3 MPa for PEUU-4MBP and PEUU-4FBP, respectively. It was also found that PEUU-nMBP and PEUU-nFBP, adsorb both bovine serum albumin and human serum gamma-globulin with a single layer. In cell culture test, the PEUUs films showed the adhesiveness of mouse fibroblast(L-929). Because of their mechanical and biocompatible properties, PEUU-nMBP and PEUU-nFBP are expected to be useful materials as an artificial blood vessel.
  • Preparation and biocompatibility of polyetherurethaneureas with poly[2-(methacryloyloxy)ethyl phosphorylcholine-co-butyl methacrylate] side chain, Kazuo Sugiyama, Mitsuteru Okabe, Kohei Shiraishi, Yoshihito Kadoma, Kobunshi Ronbunshu, Kobunshi Ronbunshu, 53(8), 496 - 505, 1996
    Summary:Glycerol-terminated macromonomers Mac(MPC/BMA)-I∼III (Mn= 1.9×104, 7×103, 4×103) were obtained from radical copolymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) and butyl methacrylate (BMA) in the presence of thioglycerol with different concentrations. Polyaddition was conducted for the syntheses of a series of copolyetherurethaneureas [PEU-g-P(MPC/BMA)-I∼III] grafted on the copolymer of MPC and BMA with the molar ratio of 0.1 : 0.9 : 2 : 1 for Mac(MPC/BMA)-I∼III : polytetrahydrofuran (PTHF) : 4,4′-diphenylmethane diisocyanate (DPMDI) : ethylene diamine. Parent polyetherurethaneurea (PEU) and poly(butyl methacrylate) graft copolyetherurethaneurea (PEU-g-PBMA) were also prepared. XPS spectra indicated that the MPC unit is located on the surface of PEU-g-P(MPC/BMA)-I∼III. The measurements of equilibrium water content and contact angle to water confirmed that the higher molecular weight of side chains results in higher hydrophilicity of PEU-g-P(MPC/ BMA)-I∼III surface. The tensile modulus showed values of E=13.3∼38.4 MPa for PEU-g-P(MPC/BMA)-I∼III films. It was also found that PEU-g-P(MPC/BMA)-I∼III suppressed the adsorption of both bovine serum albumin and human serum r-globulin less than PEU and PEU-g-PBMA. In cell culture test, PEU-g-P(MPC/BMA)-I, III films showed no adhesiveness of mouse fibroblast (L-929). Because of their mechanical and biocompatible properties, PEU-g-P(MPC/BMA)-I∼III are expected to be useful as novel biomaterials.
  • Molecular Assembly of Glycolipid Analogs Having Polymerizable Group in Nonaqueous Media, Kohei Shiraishi, Tsutomu Nakamura, Kazuo Sugiyama, Nippon Kagaku Kaishi, Nippon Kagaku Kaishi, 1995(11), 925 - 928, 1995
    Summary:3-O-[(E)-3-(Hexadecyloxycarbonyl)acryloyl]-αpha -D-glucopyranose (gHDF) and 3-0-[(E)-3-(hexadecyloxycarbonyl)acryloyl] —1,2: 5,6-di-O-isopropylidene-αpha -D-glucofuranose (digHDF) were synthesized as a glycolipid analog having polymerizable group. gHDF and digHDF were polymerized in the presence of αpha ,αpha ′.-azobisisobutyronitrile to give polymers [poly (gHDF) and poly (digHDF)]. The molecular assembly of gHDF and digHDF was examined by the solubilization of sodium 8-anilino-l-naphthalenesulfonate (ANS) as a fluorescence probe. From the relationship between the concentration of glycolipid analogs and the fluorescence intensity, a sharp increase in fluorescence intensity for all of analogs was observed in chloroform at 10.0 x 10-3, 0.8 x 10-3, 2.5 x 10-3, and 1.5 x 10-3 mol-L-1 for gHDF, poly (gHDF), digHDF, and poly (digHDF), respectively. Therefore, glycolipid analogs obtained were found to form a molecular assembly in chloroform. © 1995, The Chemical Society of Japan. All rights reserved.
  • Synthesis and properties of terminal-terminal type of smectic liquid crystals, SHIRAISHI Kohei, Chemistry Express, Chemistry Express, 8(3), 189 - 192, 1993 , Refereed
  • Synthesis and Polymerization of 12-meth-acryloyloxydodecyl 2-(dimethyl-hexadecylammonio)ethylphosphate, SHIRAISHI Kohei, Chemistry Express, Chemistry Express, 7(4), 261 - 264, 1992 , Refereed
  • Electrooptical Effect of αpha;-OIefinic Smectic Liquid Crystals, Kazuo Sugiyama, Yoshifumi Morikawa, Kohei Shiraishi, Nippon Kagaku Kaishi, Nippon Kagaku Kaishi, 1991(3), 230 - 234, 1991 , Refereed
    Summary:Three kinds of αpha -olefinic liquid crystals, 4-[4-[(5)-2-methylbutoxycarbonyl]phenoxycar-bonyl]phenyl 10-undecenoate 1, 4-[4-[(S)-2-methylbutoxycarbonyl]phenyl]plienyl 10-undecenoate 2, and (S)-2-methylbutyl 4-[N-4-(10-undecenoyloxy)benzylideneamino]cinnamate 3 were synthesized as reactive monomers for ferroelectric liquid crystalline comb-like polysiloxa-nes. 1 shows a smectic A(SA) state, while 2 and 3 show both SA and chiral smectic C (Sc∗) state. From the electrooptical measurements, the response times for dual frequency drive in SA∗ state were found to be 30, 310, and 30 ms at 0.1 Hz: 2.12, 7, and 2. 67 s at 1 kHz for 1, 2 and 3, respectively. The response time for SSFLC in Sc∗ phase was also found to be 460 and 105 μ s for 2 and 3, respectively. © 1991, The Chemical Society of Japan. All rights reserved.
  • Synthesis and Properties of Oligomer Carrying 2-8-ButyI-4,6-dinitrophenyl Group in the Side Chain, Kazuo Sugiyama, Kohei Shiraishi, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, 1990(8), 876 - 879, 1990 , Refereed
    Summary:2-s-Butyl-4, 6-dinitrophenol (BDNP) was chemically modified to polymerizable amphiphile, [10-(2-s-butyl-4,6-dinitrophenoxycarbonyl)deeyl] [2-(methaeryloyloxy)ethyl]dimethylammo-nium bromide via 2-s-butyl-4,6-dinitrophenyl 11-bromoundecanoate 1. 2 was converted to a,oligomer 3 with ca. 6500 of molecular weight by a radical polymerization. Krafft point of 2 was found to be 32. 3°C. CMC values of 2 and 3 were found to be 2.7xl0-4 and 4.0xl0-4 mpl//, respectively by the measurements of conductivity of their aqueous solution. 1, 2, and 3 exhibit antibacterial activity against Staphylococcus. © 1990, The Chemical Society of Japan. All rights reserved.
  • Different antifouling effects of random and block copolymers comprising 2-methacryloyloxyethyl phosphorylcholine and dodecyl methacrylate, Chie Kojima, Risa Katayama, Thi Lien Nguyen, Yuto Oki, Ayako Tsujimoto, Shin-ichi Yusa, Kohei Shiraishi, Akikazu Matsumoto, European Polymer Journal, European Polymer Journal, 136, 109932 - 109932, Aug. 2020 , Refereed
  • Characterization of the Hydration Process of Phospholipid-Mimetic Polymers Using Air-Injection-Mediated Liquid Exclusion Methods, Risa Katayama, Nobuyuki Tanaka, Yusuke Takagi, Kohei Shiraishi, Yo Tanaka, Akikazu Matsumoto, Chie Kojima, Langmuir, Langmuir, 36(20), 5626 - 5632, May 26 2020 , Refereed
  • Comparison of the neurotoxicities between volatile organic compounds and fragrant organic compounds on human neuroblastoma SK-N-SH cells and primary cultured rat neurons, Yasue Yamada, Kohei Ohtani, Akinori Imajo, Hanae Izu, Hitomi Nakamura, Kohei Shiraishi, Toxicology Reports, Toxicology Reports, 2, 729 - 736, Jan. 01 2015 , Refereed
    Summary:These are many volatile organic compounds (VOCs) that are synthesized, produced from petroleum or derived from natural compounds, mostly plants. Fragrant and volatile organic compounds from plants have been used as food additives, medicines and aromatherapy. Several clinical and pathological studies have shown that chronic abuse of VOCs, mainly toluene, causes several neuropsychiatric disorders. Little is known about the mechanisms of neurotoxicity of the solvents. n-Octanal, nonanal, and 2-ethyl-1-hexanol, which are used catalyzers or intermediates of chemical reactions, are released into the environment. Essential oils have the functions of self-defense, sterilization, and antibiosis in plants. When volatile organic compounds enter the body, there is the possibility that they will pass through the blood-brain barrier (BBB) and affect the central nervous system (CNS). However, the direct effects of volatile organic compounds on neural function and their toxicities are still unclear. We compared the toxicities of n-octanal, nonanal and 2-ethyl-1-hexanol with those of five naturally derived fragrant organic compounds (FOCs), linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and n-phenethyl alcohol. MTT assay of human neuroblastoma SK-N-SH cells showed that the IC< inf> 50< /inf> values of linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and phenethyl alcohol were 1.33, 2.3, > 5, > 5, and 2.39mM, respectively, and the IC< inf> 50< /inf> values of toluene, n-octanal, nonanal and 2-ethyl-1-hexanol were 850, 37.2, 8.31 and 15.1μM, respectively. FOCs showed lower toxicities than those of VOCs. These results indicate that FOCs are safer than other compounds.
  • Preparation of Poly[(meth)acrylamide)] Having L-Lysine Moiety and its Effect on Fibrinolytic Activity, Kohei Shiraishi, Sinko Wakisaka, Junji Satozaki, Kazuo Sugiyama, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 69(1), 39 - 46, Jan. 2012 , Refereed
    Summary:In order to develop a novel biomaterial showing antithrombogenisity, we prepared zwitterionic poly[N-alpha-acrylamide-L-lysine] [poly (alpha-LysAA)] or poly[N-alpha-methacrylamide-L-lysine] [poly-(alpha-LysMA)] with a selective binding activity to fibrinolytic proteins [plasminogen (Plg), tissue-type plasminogen activator (t-PA)]. Surface Plasmon Resonance (SPR) measurements on poly (alpha-LysAA) immobilized sensor surfaces found that Plg and t-PA were strongly bound to poly (alpha-LysAA) for Plg with 3.89 x 10(-7) M-1 for t-PA with 1.55 x 10(-7) M-1 as a binding constant, respectively, while other serum proteins such as albumin, gamma-globulin, and fibrinogen were weakly bound. The competitive adsorption of albumin and Plg on the immobilized polymer was also carried out. A specific interaction was found between poly (alpha-LysAA) and Plg. The maximam rate constant for the hydrolysis of chromogenic plasmin substrate (S-2251) by Plg/t-PA in the presence of poly (alpha-LysAA) was found to be V-max = 20.0 x 10(-3) mM.min(-1). The enhanced enzymatic reaction of Plg/t-PA and S-2251 was also observed in the presence of poly (alpha-LysAA) or poly (alpha-LysMA).
  • Grafting of Biocompatible Polymers on DLC Thin Films by Plasma Irradiation-Post Polymerization Technique for Application of Biomedical Devices and Cell Microarrays, Ryoji Hamawaki, Tatsuya Ishihara, Akihiro Tominaga, Kohei Shiraishi, Kazuo Sugiyama, Yuki Nitta, Tatsuyuki Nakatani, Keishi Okamoto, JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 24(4), 447 - 452, 2011
    Summary:Two-step surface modification of metal or glass substrates has been developed for the long-term reliability and safety of biomedical devices (stents, catheters, blood contacting parts,etc) and cell microarrays for cell diagnosis and cell recovery. First step, carboxylic group (COOH)s containing DLC (DLC/COOH) were coated on an aluminum surface by a plasma enhanced chemical vapor deposition (PECVD). Second step, an anti-thrombogenic, poly(N-alpha-(methacrylamide-L-lysine) (PLysMA) or poly[2-(methacryloyloxy)ethyl phosphorylcholine] (PMPC) was grafted on the DLC/COOH surface by plasma irradiation-post polymerization technique.Furthermore, to develop novel cell microarrays having regulatory-arranged glass spots and coated the surface with DLC/COOH around the spots for evaluation of cell function and selection of targeting cells by immobilization of single cells on the spots, the microarrays were also grafted with PMPC on the DLC/COOH by the plasma irradiation-post polymerization technique. Surface biocompatibility and characterization were examined by SPM, XPS, and adsorption of plasma proteins.
  • Enhancement of fibrinolytic activity in vascular endothelial cells by heterologous expression of adenine nucleotide translocase-1, Chikako Ishida, Shigeru Ueshima, Nobuo Nagai, Naoyuki Kawao, Kiyotaka Okada, Hou Yongzhong, Kohei Shiraishi, Osamu Matsuo, BLOOD COAGULATION & FIBRINOLYSIS, BLOOD COAGULATION & FIBRINOLYSIS, 21(3), 272 - 278, Apr. 2010
    Summary:The fibrinolytic activity of blood is regulated by expressing tissue-type plasminogen activator (t-PA) and its specific inhibitor, type-1 plasminogen activator inhibitor (PAI-1), from vascular endothelial cells. Since t-PA is a major plasminogen activator in blood, it is considered that the binding protein for t-PA, which exists on endothelial cell membrane, immobilizes t-PA on the surface of endothelial cells and enhances their antithrombotic property. Recently, we have found a new t-PA binding protein in endothelial cells. Its amino acid sequence has matched that of human adenine nucleotide translocase-1 (ANT1). The aims of this study are to confirm the binding of t-PA to ANT1, and to clarify the effect of ANT1 on fibrinolytic activity around endothelial cells. ANT1 is prepared from recombinant glutathione S-transferase (GST)-ANT1 fusion protein, and reveals t-PA binding activity in a ligand blot assay. In addition, ANT1 is exclusively expressed on endothelial cell membrane by using pDisplay vector. Interaction of t-PA with ANT1, which is expressed on the surface of endothelial cells, is confirmed by IAsys binding analysis and chromogenic assay. The heterologous expression of ANT1 on endothelial cell membrane enhances the t-PA binding ability of endothelial cells and the effect of ANT1 expression on fibrinolytic activity is demonstrated by increasing t-PA-catalyzed plasminogen activation. These results suggest that a novel t-PA-binding protein, ANT1, may concentrate t-PA on the surface of cells and enhance fibrinolytic properties around endothelial cells; therefore, ANT1 can be a powerful tool for regulating the plasminogen activation system in the vessel. Blood Coagul Fibrinolysis 21:272-278 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
  • Self-Organization of Amphiphilic Poly[2-(methacryloyloxy)ethyl phosphorylcholine] Carrying Tocopheryl Moieties as Terminal Groups, Nobuyuki Tanigawa, Kohei Shiraishi, Tadashi Abe, Kazuo Sugiyama, JOURNAL OF APPLIED POLYMER SCIENCE, JOURNAL OF APPLIED POLYMER SCIENCE, 113(2), 959 - 965, Jul. 2009 , Refereed
    Summary:Poly [2-(methlacryloyloxy)ethyl phosphorylcholine](PMPC) with one pendant tocopheryl moiety at the polymer terminus (PMPC-Toco) was prepared by the radical polymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) initiated with 4,4'-azobis[(3-tocopheryl)-4-cyanopentanoate] in the presence of 2-mercaptoethanol as a chain transfer reagent. The self-organization of PMPC-Toco was analyzed with fluorescence and (1)H-NMR measurements. The critical micelle concentrations of PMPC-Toco with [eta] = 0.25, 0.13, 0.1.0, and 0.05 dL g(-1) were found to be 200, 100, 100, and 90 mg L(-1), respectively. The blood compatibility of PMPC-Toco was evaluated from the Michaelis constant (K for the enzymatic reaction of thrombin and a synthetic substrate, S-2238, in the presence of PMPC-Toco. The K values were 0.21, 0.23, 0.36, and 0.21 for PMPC-Toco-1, 2, 3, and PMPC ([eta] = 0.56 dL g(-1)), respectively. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 959-965, 2009
  • Blood Compatibility of Self-Assembiled Poly (N-alpha-methacrylamide-L-lysine-b-dimethylsiloxane) Copolymers, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 65(2), 150 - 156, 2008 , Refereed
    Summary:Block copolymers, poly (LysMA-b-DMS)-1 similar to 3 with different molecular weight, were prepared by the conversion of poly[N-alpha-methacrylamide-N-epsilon-t-butoxycarbonyl-L-lysin-t-butyl ester (BLMA)-b-dimethylsiloxane (DMS)] which were obtained from the radical polymerrization of BLMA initiated with an azo-initiator having poly-dimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly (LysMA-b-DMS) showed microphase-separated structures. The self-organization of poly (LysMA-b-DMS) was analyzed by fluorescence and H-1 NMR measurements. The critical micelle concentrations (CMC) were = 500-2000 mg L-1. The micelle had diameters from 221 to 274 nm. The blood compatibility of poly (LysMA-b-DMS) was evaluated from the maximum velocity of hydrolysis (V-max) for the enzymatic reaction of thrombin and a synthetic substrate S-2238 in the presence of poly (LysMA-b-DMS). V-max was 1.82 x 10(-2)similar to 1.95 x 10(-2) mM min(-1). The maximum rate of conversion of plasminogen to plasmin by tissue plasminogen activator (t-PA) in the presence of poly (LysMA-b-DMS) using S-2251 was faster than the case of absence of poly (LysMA-b-DMS).
  • Evaluation of Surface Characteristic of Poly (ethylene terephthalate) Film Modified with Thermoresponsible Polymers Using Bacterial Random Peptide Library Method, Kohei Shiraishi, Tetsuyuki Shigesada, Kazuhiko Yamamoto, Kazuo Sugiyama, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 65(2), 132 - 139, 2008 , Refereed
    Summary:In order to develop a more precise evaluation method for the application as a cell scaffold, the surface of thermoresponsible poly (N-isopropylacrylamide) (PNiPAAm) grafted PET (g-PET) was characterized by a bacterial random peptide library method (FliTrx (TM): Invitrogen). The g-PET was prepared by an Ar plasma-post polymerization method. From the analysis of selective 12 amino acids sequences, we could observe that the surface of g-PET was hydrophilic at 25 degrees C and hydrophobic surface at 37 degrees C, respectively. In addition, the Kyte & Doolittle parameter of g-PET was -67.6 on hydrophilic surface at 25 degrees C and -28.4 for the hydrophobic surface at 37 degrees C. On the other hand, the Kyte & Doolittle parameter of the untreated PET was 34.3 at 25 degrees C and 42.2 at 37 degrees C, respectively. The Selection of amino acids sequences changed drastically on the g-PET by raising the panning temperature from 25 degrees C to 37 degrees C.
  • Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice, Sayuri Otaki, Shigeru Ueshima, Kohei Shiraishi, Kazuo Sugiyama, Suguru Hamada, Masatomo Yorimoto, Osamu Matsuo, CELL BIOLOGY INTERNATIONAL, CELL BIOLOGY INTERNATIONAL, 31(10), 1191 - 1197, Oct. 2007 , Refereed
    Summary:The technique of tissue engineering is developing for the restoration of lost tissues. This new technique requires cells that fabricate tissue. Mesenchymal stem cells in bone marrow have been used as the cell source for this technique; however, dental pulp cells have recently been shown to possess stem-cell-like properties. We earlier demonstrated that dental pulp cells proliferate and produce an extracellular matrix that subsequently becomes mineralized in vitro. We now report that such dental pulp cells (first to eighth passage) produced bone instead of dentin when those cells were implanted into subcutaneous sites in immunocompromised mice with HA/TCP powder as their carrier. This evidence shows that dental pulp cells are the common progenitors of odontoblasts and osteoblasts, or dental pulp cells are mesenchymal stem cells themselves. It is expected that dental pulp cells can be a useful candidate cell source for tissue engineering, and contain the potential of new therapeutic approaches for the restoration of damaged or diseased tissue. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
  • Cholesteryl moiety terminated amphiphilic polymethacrylates containing nucleic acid bases for drug delivery, Kohei Shiraishi, Minoru Sugiyama, Yoji Okamura, Kazuo Sugiyama, JOURNAL OF APPLIED POLYMER SCIENCE, JOURNAL OF APPLIED POLYMER SCIENCE, 103(5), 3064 - 3075, Mar. 2007 , Refereed
    Summary:Poly[9-(2-methacryloyloxyethyl)adenine] and poly[1-(2-methacryloyloxyethyl)thymine] with one pendant cholesteryl moiety at the polymer end (PMEA-Chol, PMET-Chol) and with two pendant cholesteryl moieties at both polymer ends as terminal groups (PMEA-2Chol, PMET-2Chol) were prepared by radical polymerization of 9-(2-methacryloyl-oxyethyl)adenine (MEA) and 1-(2-methacryloyloxyethyl)thymine (MET) initiated with 4,4'-azobis[(3-cholesteryl)4-cyanopentanoate] in the presence of 2-mercaptoethanol or thiocholesterol as chain transfer reagents, respectively. The copolymers [PNiPAAm-co-PMEA-nChol (n = 1,2)] composed of N-isopropylacrylamide (NiPAAm) and MEA were also prepared in a similar manner. The self-organization of these polymers and copolymers was confirmed by a fluorescence measurement, and then their critical concentrations of micelle formation (CMC) were determined. The mixture of PMEA-2Chol and cholesterol as a lipophilic drug model formed a lamella type of complex with an interplaner spacing of d = 35.3 angstrom. The hypochromism based on the formation of a 1 : I interaction of adenine and thymine moieties was found to appear in the mixed aqueous solution of PMEA-Chol and PMET-Chol. Complementary interactions were also confirmed in the system of PMET-2Chol and adenosine as well as PMEA-2Chol and uridine. Cis-dichlorodiammine platinum(II) (CDDP) was bound to PNiPAAm-co-PMEA-Chol through the adenine moiety by ligand substitution atoms of CDDP. The amount of CDDP loaded on the copolymer was found to be 0.143 g g(-1). (c) 2006 Wiley Periodicals, Inc.
  • Molecular assembly and blood compatibility of poly[2-(methacryloyloxy)ethyl phosphorylcholine-b-dimethylsiloxane] block copolymers, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 64(6), 373 - 379, 2007 , Refereed
    Summary:Block copolymers, poly (MPC-b-DMS)-I-IV, were prepared by the radical polymerization of 2-(methacryloyloxy) ethyl phosphorylcholine (MPC) initiated with an azo-initiator having polydimethylsiloxane chains (azo-PDMS) in the presence of 2-mercaptoethanol as a chain-transfer agent. From scanning probe microscope (SPM) measurements, poly(MPC-b-DMS) was found to form microphase-separated structures. The self-organization of poly(MPC-b-DMS) was analyzed by fluorescence and H-1 NMR measurements. The critical micelle concentrations of poly (MPC-b-DMS) were 90-600 mg L-1. The blood compatibility of poly (MPC-b-DMS) -I-IV was evaluated from the Michaelis constant (K-m) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238. K-m was found to be 0.10-0.16 mM.
  • Preparation of thermo-sensitive polymer-grafted poly(ethyleneterephthalate) films by Ar plasma irradiation-post polymerization technique and thermal stimuli-exfoliation of HeLa cells on their films, Kohei Shiraishi, Megumi Ohdan, Kazuma Maeda, Kazuo Sugiyama, Katsuyuki Suzuki, Hiroshi Hosoya, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 63(9), 613 - 620, 2006 , Refereed
    Summary:In order to develop a novel cell cultured material capable of thermal stimulating cell detachment from its surface, we studied the biocompatible and thermosensitive copolymer consisting of N-(2-hydroxypropyl)methacrylamide(HPMA) and methyl methacrylate(MMA) [poly(HPMA-co-MMA): HPMA/MMA = 100/27.6 (molar ratio)]. With N-isopropylacylamide (NiPAAm) as a control of thermosensitive polymer, the copolymers were grafted onto poly (ethyleneterephthalate) (PET) by Ar Plasma irradiation-post polymerization technique. From the contact angle (theta) measurements to water at 25 degrees C, the theta values of poly (HPMA-co-MMA) grafted PET (g-PET-1: amount of grafted polymer = 0.125 mg cm(-2)) and poly (NiPAAm) grafted PET (g-PET-II: 0.109 mg cm(-2)) decreased from theta = 80 degrees (the original PET) to theta = 52 degrees and theta = 50 degrees, respectively. HeLa cells attached and proliferated on g-PET-I and g-PET-II at 37 degrees C, above the lower critical solution temperature of poly (HPMA-co-MMA). After the cultivation of HeLa cells, the thermal stimuli-exfoliation was also examined at 4 degrees C for 2 h. The ratios of the number of thermal stimuli-exfoliation cells to the total number of attached cells on g-PET-I or g-PET-II were 73% and 45%, respectively, while little thermal stimuli-exfoliation was observed in the case of untreated PET.
  • Preparation of zwitterionic polymethacrylamide modified with L-lysine and its effect on fibrinolytic activity, K Shiraishi, M Kohta, K Sugiyama, CHEMISTRY LETTERS, CHEMISTRY LETTERS, 33(6), 646 - 647, Jun. 2004 , Refereed
    Summary:Zwitterionic polymethacrylamides, poly(N-alpha-methacrylamide-L-lysine) [poIy(alpha-LysMA)] and poly(N-epsilon-methacrylamide-L-lysine) [poly(epsilon-LysMA)] were prepared to examine their bioactivity by an evaluation of fibrinolytic activity and a binding assay using resonant mirror biosensor (IAsys). Poly(alpha-LysMA) enhanced the fibrinolytic activity by plasminogen/tissue-type plasminogen activator while no enhancement was observed in the case of poly(epsilon-LysMA). A strong interaction between Pig to poly(alpha-LysMA) was also observed by IAsys when compared with poly(epsilon-LysMA).
  • Characterization and application of polypropylene films modified with stimuli-sensitive copolymers with an Ar-plasma postpolymerization technique, K Sugiyama, K Shiraishi, K Sono, JOURNAL OF APPLIED POLYMER SCIENCE, JOURNAL OF APPLIED POLYMER SCIENCE, 90(1), 143 - 148, Oct. 2003 , Refereed
    Summary:Surface-modified polypropylene (PP) films with thermally and photochemically sensitive copolymers consisting of N-(2-hydroxypropyl)methacrylamide (HPMA) and 4-(4-methoxvphenylazo)phenyl methacrylate (MPAP), poly(HPMA-co-MPAP)-g-PP (abbreviated g-PP) film, were prepared by graft copolymerization with an Ar-plasma postpolymerization technique. The surfaces of the g-PP films were characterized by means of X-ray photoelectron spectroscopy; the percentage grafting of poly(HPMA-co-MPAP) with a number-average molecular weight of 3.28 x 10(4) was 7.12%, and the molar ratio of HPMA-MPAH in the copolymer was 0.75:0.25. The stimuli-sensitive adsorption of albumin and polystyrene microspheres on the g-PP film was also measured. (C) 2003 Wiley Periodicals, Inc.
  • Assembly of amphiphilic poly[2-(methacryloyloxy)ethyl phosphorylcholine] with cholesteryl moieties as terminal groups, K Sugiyama, K Shiraishi, T Matsumoto, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 41(13), 1992 - 2000, Jul. 2003 , Refereed
    Summary:Poly[2-(methacryloyloxy)ethyl phosphoryleholine]s (PMPCs) with one pendant cholesteryl moiety at the polymer end (PMPC-Chol-I and PMPC-Chol-II) and two pendant cholesteryl moieties at both polymer ends as terminal groups (PMPC-2Chol-I and PMPC-2Chol-II) were prepared by the radical polymerization of 2-(methacryloyloxy)ethyl phosphorylcholine initiated with 4,4'-azobis[(3-cholesteryl)-4-cyanopentanoate] in the presence of 2-mercaptoethanol or thiocholesterol as chain-transfer reagents, respectively. The self-organization of PMPC-Chol and PMPC-2Chol was analyzed with fluorescence and H-1 NMR measurements. The critical micelle concentrations of PMPC-Chol-I with a degree of polymerization (P-n) of 91 and of PMPC-2Chol-I with a P-n value of 165 were 250 and 27 mg L-1, respectively. The blood compatibility of PMPC-2Chol was evaluated from the Michaelis constant (K-m) for the enzymatic reaction of thrombin and a synthetic substrate, S-2238, in the presence of PMPC-2Chol. K-m was 0.07, 0.05, and 0.56 for PMPC-2Chol-I with P-n = 165, PMPC-2Chol-II with P-n = 38, and PMPC (an intrinsic viscosity of 0.54 dL g(-1)) initiated with 2,2'-azobisisobutyronnitrile in the absence of chain transfer agent, respectively. A mixture of PMPC-2Chol-II and cholesterol as a drug. model formed a lamellar type of complex with an interplanar spacing of d = -35.2 Angstrom. (C) 2003 Wiley Periodicals, Inc.
  • Analysis of pH response for amphoteric poly(O-methacryloyl-L-serine) and interaction with serum protein by fluorescence spectroscopy, K Shiraishi, K Miura, G Asami, M Kohta, K Sugiyama, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 60(1), 30 - 37, 2003 , Refereed
    Summary:The amphoteric polymer having L-serine residues in the side chain as a pH responsible hydrogel component was prepared as follows: L-serine with protected amino and carboxyl groups, N-tert-butoxycarbonyl-O-methacryloyl-L-serine diphenylmethyl ester (Boc-SerMA-Ph-2) and 2-(dansyloxy) ethyl methacrylate (DnsEMA) were copolymerized by a radical mechanism with 2,2'-dimethyl-azobisisobutyrate in tetrahydrofuran at 60 degreesC for 20 h. Deprotection of the resulting polymer was carried out in 80% trifluoroacetic acid aqueous solution to give amphoteric poly(SerMA) labeled with a dansyl group [poly(SerMA-co-DnsEMA)]. The fluorescence spectral changes of a dansyl-labeled poly(SerMA) solution at pH ranging from 1.2 to 12.0 revealed that poly(SerMA) shows pH response. The interaction between a serum protein such as bovine serum albumin (Alb), human gamma-globulin (Glo), or bovine serum fibrinogen (Fib), and poly(SerMA-co-DnsEMA) was also examined by means of fluorescence spectroscopy in a phosphate buffered solution at pH 5.7 for Alb and Fib, pH 6.2 for Glo. The interaction between Alb and poly(SerMA-co-DnsEMA) was observed, whereas no definite interaction was confirmed for Glo and Fib.
  • Preparation and application of chiral recognizable thermosensitive polymers and hydrogels consisting of N-methacryloyl-s-phenylalanine methyl ester, K Sugiyama, S Rikimaru, Y Okada, K Shiraishi, JOURNAL OF APPLIED POLYMER SCIENCE, JOURNAL OF APPLIED POLYMER SCIENCE, 82(1), 228 - 236, Oct. 2001 , Refereed
    Summary:Amphiphilic random copolymers, poly(R-HPMA-co-S-PAM) and poly(H-PMA-co-S-PAM), were prepared by radical copolymerization of N-methacryloyl-(S) phenylalanine methyl ester (S-PAM) and N- [(R)-2-hydroxypropyl] methacrylamide (R-HPMA) or N-(2-hydroxypropyl)methacrylamide (HPMA) with various molar ratios of R-HPMA (or HPMA) (m) to S-PAM (n). Either aqueous solution of poly(R-HPMA-co-S-PAM) with the molar ratio of m : n = 0.81 : 0.19 or poly(HPMA-co-S-PAM) with the molar ratio of m : n = 0.79 : 0.21 exhibited the lower critical solution temperature (LCST) at 16 degreesC. The LCST in the presence of (S)-(-)-phenylalanine (S-Phe) shifted from 16 to 20 degreesC and 18 degreesC for poly(R-HPMA- co-S-PAM) and poly(HPMA-co-S-PAM), respectively, whereas the LCST did not shift in the presence of(R-( +)-phenylalanine (R-Phe). Thermosensitive Gel(R-HPMA-co-S-PAM) and Gel(HPMA-co-S-PAM) were also prepared from radical copolymerization of S-PAM and R-HPMA or HPMA in the presence of N,N ' -ethylenebisacrylamide (EBAAm) as a crosslinker. When the gels shrunk at 40 degreesC, the release of dansyl-(R)-phenylalanine (Dans-R-Phe) from the gel in which loaded Dans-R-Phe occurred was more easily done than that of Dans-S-Phe from the gel that loaded Dans-S-Phe. Thus, these thermosensitive copolymers and gels were found to exhibit chiral recognition for phenylalanine derivatives. (C) 2001 John Wiley & Sons, Inc.
  • Biocompatible block copolymers composed of polydimethylsiloxane and poly[(2-methacryloyloxy)ethyl phosphorylcholine] segments, K Sugiyama, K Shiraishi, K Okada, O Matsuo, POLYMER JOURNAL, POLYMER JOURNAL, 31(10), 883 - 886, 1999 , Refereed
  • Preparation of poly(methyl methacrylate) microspheres modified with amino acid moieties, K Shiraishi, T Ohnishi, K Sugiyama, MACROMOLECULAR CHEMISTRY AND PHYSICS, MACROMOLECULAR CHEMISTRY AND PHYSICS, 199(9), 2023 - 2028, Sep. 1998 , Refereed
    Summary:Poly(methyl methacrylate) microspheres [poly(SerMA-co-MMA), poly(AlaMAm-co-MMA), poly(AlaEMA-co-MMA, and poly(AlaMAm/AlaEMA-co-MMA)] modified with O-methacryloyl-L-serine (SerMA), N-methacryloyl-L-alanine (AlaMAm) and L-alanine 2-methacryloyloxyethyl ester (AlaEMA) were prepared by the emulsifier-free emulsion copolymerization of methyl methacrylate (MMA) with SerMA, AlaMAm and AlaEMA, respectively, initiated with 2,2'-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (ABIP). The series of polymer microspheres showed unimodal distribution of the particle size (290-800 nm) in water. From X-ray photoelectron spectroscopy it was concluded that the amino acid moieties are located on the surface of the particles in all cases. Poly(SerMA-co-MMA) showed the most effective suppression of adsorption of proteins such as albumin (Alb), gamma-globulin (Glo) and fibrinogen (Fib) among the examined poly(methylmethacrylate) microspheres.
  • Characterization of polydimethylsiloxane block copolymers containing hydrophilic polymethacrylate segments, K Sugiyama, N Tanigawa, K Shiraishi, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (8), 551 - 557, Aug. 1998 , Refereed
    Summary:A series of block copolymers, i.e., PHEMA-b-PDMS, PDMM-b-PDMS and PMDID-b-PDMS, were obtained from polymerization of 2-hydroxyethyl methacrylate (HEMA), 2,2-dimethyl-1,3-dioxolan-4-ylmethyl methacrylate (DMM) and 5-(5-hydroxymethyl-2,2-dimethyl-1,3-dioxolan-4-yl)-2,2-dimethyl-1,3-dioxolan-4-ylmethyl methacrylate (MDID) initiated with polydimethylsiloxane type of ate-initiator (VPS). Conversion of PDMM segment in PDMM-b-PDMS and PMDID segment in PMDID-b-PDMS into poly(2,3-dihydroxypropyl methacrylate) (PDHPMA) and poly(2,3,4,5,6-pentahydroxyhexyl methacrylate) (PMDul) segments by hydrolysis with a dilute hydrochloric acid resulted in PDHPMA-b-PDMS and PMDul-b-PDMS, respectively. From XPS measurements of freeze dried PHEMA-b-PDMS, PDHPMA-b-PDMS and PMDul-b-PDMS samples, the hydrophilic segment was found to migrate to the surface of copolymer films in water. The contact angle of water of copolymer films decreased with increasing the amount of the hydrophilic segment. The copolymer with a hydrophilic surface suppressed the adsorption of albumin (Alb) and gamma-globulin (Glo) as well as platelets, less than the polydimethylsiloxane as control.
  • Grafting of vinyl monomers on the surface of a poly(ethylene terephthalate) film using Ar plasma-post polymerization technique to increase biocompatibility, Kazuo Sugiyama, Kazuya Kato, Masakazu Kido, Kohei Shiraishi, Koji Ohga, Kiyotaka Okada, Osamu Matsuo, Macromolecular Chemistry and Physics, Macromolecular Chemistry and Physics, 199(6), 1201 - 1208, 1998 , Refereed
    Summary:Surface modified poly(ethylene terephthalate) (PET) films with 2-(methacryloyloxy)ethylphosphorylcholine (MPC) and 2-(glucosyloxy)ethyl methacrylate (GEMA) moieties, PMPC-g-PET and PGEMA-g-PET, were prepared by graft copolymerization using an Ar plasma-post polymerization technique. The degrees of polymerization of the grafts PMPC and PGEMA were P̄n ≈ 30 and P̄n ≈ 40, respectively. The contact angle of the modified PET film decreased from θ = 68° (the original PET film) to θ = 26° for PMPC-g-PET and to θ = 43° for PGEMA-g-PET. The modified PET films adsorb less serum proteins than the original PET film. Egg yolk lecithin did not adsorb on PGEMA-g-PET but adsorbed on PMPC-g-PET. PMPC-g-PET showed activity for the inhibition of fibrin formation and no adhesion of mouse fibroblasts (L-929).
  • Introduction of tetramethyldisiloxane moiety into polyetherurethaneurea, K Sugiyama, K Isobe, K Shiraishi, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (11), 816 - 820, Nov. 1997 , Refereed
    Summary:Polyetherurethaneureas (PEUU-Si) including the tetramethyldisiloxane moiety in main chain were obtained from a typical two step polyaddition of polytetrahydrofuran (PTHF) TO 4,4'-methlenebis(phenyliscyanate) (MPI) in the presence of 1,3-bis(3-hydroxypropyl)-1,1,3,3-tetramethyldisiloxane (TMDS), using ethylenediamine (EDA) as a chain extension reagent. Polyaddition with a molar ratio of 1:2:1 for PTHF+TMDS:MPI:EDA gave the PEUU-Si in the mixed solvent of dimethyl sulfoxide and isobutyl methyl ketone, where the molar ratio of TMDS was changed from 0.2 to 1.0. From the XPS spectra and the contact angle measurements, it was found that the TMDS moiety was located on the surface of the PEUU-Si film in air and hydrophobicity was increased with increasing the molar ratio of the TMDS moiety. It was also found that PEUU-Si adsorbed bovine serum albumin. The tensile modulus of PEUU-Si showed E=66-120 MPa.
  • Adsorption of protein on the surface of thermosensitive poly(methyl methacrylate) microspheres modified with the N-(2-hydroxypropyl)methacrylamide and 2-(methacryloyloxy)ethyl phosphorylcholine moieties, K Sugiyama, S Mitsuno, K Shiraishi, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 35(16), 3349 - 3357, Nov. 1997 , Refereed
    Summary:A series of microspheres composed of methyl methacrylate (MMA) and N(2-hydroxypropyl) methacrylamide (HPMA), and/or 2-(methacryloyloxy)ethyl phosphorylcholine (MPC), i.e., binary copolymer microspheres [poly(HPMA-co-MMA)(KPS) and poly(HPMA-co-MMA)(ABIP)] and ternary ones [poly(HPMA/MPC-co-MMA)(KPS) and poly(HPMA/MPC-co-MMA)(ABIP)], were prepared by emulsifier-free emulsion copolymerization using potassium peroxodisulfate (KPS) or 2,2'-azobis [2-(imidazolin-2-yl)propane]dihydrochloride (ABIP) as initiators. The decrease in zeta-potential of the polymer microspheres is caused by the addition of the HPMA and/or MPC moieties. Equilibrium water content of poly(HPMA-co-MMA)(ABIP) showed a remarkable swelling change with a change in response to temperature: the hydrated conformation at 28 degrees C and the dehydrated one at above 40 degrees C. The adsorption of protein on the polymer microspheres also changed in response to change in temperature. The ternary polymer microspheres effectively suppressed the adsorption both of Alb and Glo, less than binary ones. A series of polymer microspheres are expected to apply as a novel drug carrier with both thermosensitive and nonthrombogenic functions. (C) 1997 John Wiley & Sons, Inc.
  • Surface modified poly(methyl methacrylate) microspheres with the O-methacryloyl-L-serine moiety, K Shiraishi, T Ohnishi, K Sugiyama, K Okada, O Matsuo, CHEMISTRY LETTERS, CHEMISTRY LETTERS, (9), 863 - 864, 1997 , Refereed
    Summary:The poly(methyl methacrylate) microspheres [poly(SerMA-co-MMA)], modified their surface with novel zwitterionic O-methacryrloyl-L-serine (SerMA) were prepared bl! emulsifier-free emulsion copolymerization of SerMA and MMA. Poly(SerMA-co-MMA) suppressed the adsorption of serum protein and the activation of platelet.
  • Thermosensitive copolymers of N-(2-hydroxypropyl)methacrylamide and alkyl methacrylates, K Sugiyama, S Mitsuno, Y Yasufuku, K Shiraishi, CHEMISTRY LETTERS, CHEMISTRY LETTERS, (3), 219 - 220, 1997
    Summary:The aqueous solution of copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and alkyl methacrylate (RMA, alkyl :methyl or butyl) with molar ratio of RMA more than 0.08 was found to exhibit the LCST though no phase transition occurred for the case of the homopolymer of HPMA.
  • Preparation and biocompatibility of polyetherurethaneureas with poly[2-(methacryloyloxy)ethyl phosphorylcholine-co-butyl methacrylate] side chain, K Sugiyama, M Okabe, K Shiraishi, Y Kadoma, KOBUNSHI RONBUNSHU, KOBUNSHI RONBUNSHU, 53(8), 496 - 505, 1996
    Summary:Glycerol-terminated macromonomers Mac(MPC/BMA)-I similar to III (M(n)=1.9X10(4), 7X10(3), 4X10(3)) were obtained from radical copolymerization of 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) and butyl methacrylate (BMA) in the presence of thioglycerol with different concentrations. Polyaddition was conducted for the syntheses of a series of copolyetherurethaneureas [PEU-g-P(MPC/BMA)-I similar to III] grafted on the copolymer of MPC and BMA with the molar ratio of 0.1:0.9:2:1 for Mac(MPC/BMA)-I similar to III:polytetrahydrofuran (PTHF):4,4'-diphenylmethane diisocyanate (DPMDI):ethylene diamine. Parent polyetherurethaneurea (PEU) and poly(butyl methacrylate) graft copolyetherurethaneurea (PEU-g-PBMA) were also prepared. XPS spectra indicated that the MPC unit is located on the surface of PEU-g-P(MPC/BMA)-I similar to III. The measurements of equilibrium water content and contact angle to water confirmed that the higher molecular weight of side chains results in higher hydrophilicity of PEU-g-P(MPC/BMA)-I similar to III surface. The tensile modulus showed values of E=13.3 similar to 38.4 MPa for PEU-g-P(MPC/BMA)-I similar to III films. It was also found that PEU-g-P(MPC/BMA)-I similar to III suppressed the adsorption of both bovine serum albumin and human serum gamma-globulin less than PEU and PEU-g-PBMA. In cell culture test, PEU-g-P(MPC/BMA)-I, III films showed no adhesiveness of mouse fibroblast (L-929). Because of their mechanical and biocompatible properties, PEU-g-P(MPC/BMA)-I similar to III are expected to be useful as novel biomaterials.
  • MOLECULAR ASSEMBLY OF GLYCOLIPID ANALOGS HAVING POLYMERIZABLE GROUP IN NONAQUEOUS MEDIA, K SHIRAISHI, T NAKAMURA, K SUGIYAMA, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, 1995(11), 925 - 928, Nov. 1995
    Summary:3-O-[(E)-3-(Hexadecyloxycarbonyl)acryloyl]-alpha-D-glucopyranose (gHDF) and 3-O-[(E)-3-(hexadecyloxycarbonyl) acryloyl]-1,2:5,6-di-O-isopropylidene-alpha-D-glucofurano (digHDF) were synthesized as a glycolipid analog having polymerizable group. gHDF and digHDF were polymerized in the presence of alpha,alpha'-azobisisobutyronitrile to give polymers [poly(gHDF) and poly(digHDF)]. The molecular assembly of gHDF and digHDF was examined by the solubilization of sodium 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescence probe. From the relationship between the concentration of glycolipid analogs and the fluorescence intensity, a sharp increase in fluorescence intensity for all of analogs was observed in chloroform at 10.0 x 10(-3), 0.8 x 10(-3), 2.5 x 10(-3), and 1.5 x 10(-3) mol L-l for gHDF, poly(gHDF), digHDF, and poly(digHDF), respectively. Therefore, glycolipid analogs obtained were found to form a molecular assembly in chloroform.
  • Preparation and characterization of succinic acid polyesters having bisphenol moieties as hard cores, K Sugiyama, S Akita, K Shiraishi, FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS, FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS, 137 - 138, 1995 , Refereed
  • Preparation and characterization of surface modified polymer microspheres with C- and N-terminal amino acid moieties, K Shiraishi, T Onishi, K Sugiyama, FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS, FIRST EAST-ASIAN POLYMER CONFERENCE (EAPC-1) - PREPRINTS, 135 - 136, 1995 , Refereed
  • RADICAL POLYMERIZATION AND COPOLYMERIZATION REACTIVITIES OF FUMARATES BEARING DIFFERENT ALKYL ESTER GROUPS, T OTSU, K SHIRAISHI, A MATSUMOTO, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 31(10), 2523 - 2529, Sep. 1993 , Refereed
    Summary:Radical polymerization of fumarates bearing different alkyl ester groups (DRF) on the same molecules was investigated. In bulk polymerization of DRF at 60-degrees-C initiated with 2,2'-azobis (isobutyronitrile), it was confirmed that the polymerization reactivity depended on the structures of both alkyl ester groups. The introduction of bulky alkyl groups increased the polymerization rate and molecular weight of the polymer because of retardation of bimolecular termination rates. The effect of the ester substituents on the termination was examined by electron spin resonance spectroscopy. The copolymerization reactivities of DRF with styrene were also investigated. (C) 1993 John Wiley & Sons, Inc.
  • MONOMER-ISOMERIZATION RADICAL POLYMERIZATION OF DI-TERT-BUTYL MALEATE AND PREPARATION OF POLY(FUMARIC ACID) VIA PYROLYSIS, T OTSU, K SHIRAISHI, A MATSUMOTO, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 31(4), 885 - 890, Mar. 1993 , Refereed
    Summary:Di-tert-butyl maleate (DtBM) did not polymerize with 2,2'-azobis (isobutyronitrile) as a radical initiator, but DtBM easily homopolymerized via a monomer-isomerization radical polymerization mechanism to give a high molecular weight polymer when morpholine was added into the polymerization system as an isomerization catalyst. The feature of the monomer-isomerization polymerization-of DtBM was investigated in detail. The polymer obtained was confirmed to consist of a poly (tert-butoxycarbonylmethylene) structure similar to that from di-tert-butyl fumarate. Subsequent pyrolysis of the resulting polymer at 180-degrees-C is a useful route to synthesis of a high molecular weight poly(fumaric acid).
  • Thin fims plasma polymerized from propylene oxide and carbon dioxide mixture., Polym. J., Polym. J., 23(10), 1993
  • PREPARATION AND PROPERTIES OF POLYURETHANES CONTAINING MESOGENIC UNITS, K SUGIYAMA, K SHIRAISHI, K KATO, POLYMER JOURNAL, POLYMER JOURNAL, 25(1), 103 - 108, 1993
  • SYNTHESIS OF POLY(METHYL METHACRYLATE) MICROSPHERES BEARING PHOSPHATIDYLCHOLINE ANALOGOUS AND AZOBENZENE MOIETIES, K SUGIYAMA, K SHIRAISHI, K OHGA, H SHIRAHAMA, H TAMAI, K KIKUKAWA, H YASUDA, POLYMER JOURNAL, POLYMER JOURNAL, 25(5), 521 - 527, 1993
    Summary:Microsphere, Poly(MAHP-co-MMA), was prepared by the emulsifier free emulsion copolymerization of 2-[2-(methacryloyloxy)ethyldimethylammonio]ethyl-6-[4-(4-methoxyphenylazo)phenoxy]hexyloxyphosphate (MAHP) and methyl methacrylate (MMA). The diameter of poly (MAHP-co-MMA) estimated from TEM varied from 234 to 402 nm. depending on the mol-% of MAHP to MMA in feed from f = 0 to f = 0.5. From XPS measurements of poly (MAHP-co-MMA) the polar head groups of MAHP were found to be located on the surface of particles. The adsorption of bovine serum albumin (BSA) onto poly(methyl methacrylate) [poly(MMA)] and poly(MAHP-co-MMA) microspheres were examined at pH 5.6. The introduction of phosphatidylcholine analog into poly(MMA) results in a drastic decrease in BSA adsorption. A controlled decrease in BSA adsorption was also observed in poly(MAHP-co-MMA) with cis-azobenzene moieties caused by UV irradiation.
  • TERMINAL-TERMINAL TYPES OF LIQUID-CRYSTALS .3. THERMAL AND ELECTROOPTICAL BEHAVIOR OF LIQUID-CRYSTALLINE BIS[URETHANE]S, K SUGIYAMA, K KATO, K SHIRAISHI, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 65(8), 2259 - 2263, Aug. 1992
    Summary:Novel terminal-terminal types of liquid crystalline bis[urethane]s 3, prepared from (S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 2 and various diisocyanates, were studied by differential scanning calorimetry (DSC) and polarized optical microscopy (POM). Bis[urethane]s 3c obtained from 1,3-diisocyanatobenzene and 3d from 2,4-diisocyanatotoluene exhibit a smectic state, while bis[urethane]s 3a obtained from 1,6-diisocyanatohexane, 3b from bis(4-isocyanatophenyl)methane and 3e from 2,6-diisocyanatotoluene show no mesophases. It was found from IR spectroscopic results that inter- and intramolecular hydrogen bondings due to N-H and C=O of urethane bonds play an important role in the formation of mesophase. The dual-frequency drive, based on light scattering driven by the application of an a.c. current at different frequencies, was studied in the smectic A state of 3c and 3d: tau(r) at 0.1 Hz are 0.84 and 0.25 s, tau(r) at 1 kHz are 9.75 and 8.95 s for 3c and 3d, respectively.
  • EFFECT OF THE SUBSTITUENTS ON RADICAL COPOLYMERIZATION OF DIALKYL FUMARATES WITH SOME VINYL MONOMERS, T OTSU, A MATSUMOTO, K SHIRAISHI, N AMAYA, Y KOINUMA, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 30(8), 1559 - 1565, Jul. 1992
    Summary:Radical copolymerization of dialkyl fumarates (DRF) with various vinyl monomers was carried out in benzene at 60-degrees-C. The monomer reactivity ratios, r1 and r2, were determined from the comonomer-copolymer composition curves. The relative reactivity of DRFs with various ester substituents toward a polystyryl radical was revealed to depend on both steric and polar effects of the ester groups. It has also been clarified that alpha-substituents of the polymer radical have a significant role in addition of DRF, from the comparison of the monomer reactivity ratios determined in copolymerizations with monosubstituted and 1,1-disubstituted ethylenes. The absolute cross-propagation rate constants were also evaluated and discussed.
  • SYNTHESIS OF HIGH-MOLECULAR-WEIGHT POLY(DIALKYL FUMARATE)S BEARING NORMAL-ALKYL SIDE-CHAINS FROM POLY(DI-TERT-BUTYL FUMARATE) VIA OLEFIN ELIMINATION AND REESTERIFICATION IN A ONE-POT, T OTSU, M YOSHIOKA, A MATSUMOTO, K SHIRAISHI, POLYMER BULLETIN, POLYMER BULLETIN, 26(2), 159 - 164, Jul. 1991
    Summary:High molecular weight poly(dialkyl fumarate)s bearing n-alkyl esters such as an octadecyl group were prepared efficiently from poly(di-tert-butyl fumarate) by the one-pot synthesis, i.e. both elimination of isobutene from the tert-butyl ester and the subsequent esterification were performed in a corresponding alcohol at 170-180-degrees-C in a one-pot without isolating poly(fumaric acid). The resulting polymers were characterized by IR and NMR spectroscopies and GPC. The polymers with various contents of an octadecyl group in the side chain were also obtained similarly from the copolymer of di-tert-butyl fumarate with diisopropyl fumarate.
  • THERMAL-PROPERTIES OF LIQUID-CRYSTALLINE POLYITACONATES WITH PAIRED MESOGENS, K SUGIYAMA, K SHIRAISHI, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 64(5), 1715 - 1717, May 1991
    Summary:2-Methylenesuccinates (Itaconates) (2) having two 4-methoxyazobenzene moieties as paired mesogenic groups were prepared. The monomer 2 was radically homo-polymerized and copolymerized with non-mesogenic monomer, dioctadecyl itaconate (DOI). Paired mesogenic homopolyitaconate exhibits a nematic phase and the copolymers of the monomer 2 with DOI also show a mesomorphic phase up to 25 mol% of DOI content. The enhancement of mesophase formation by paired mesogens is discussed.
  • THERMAL AND ELECTROOPTICAL PROPERTIES OF FERROELECTRIC LIQUID-CRYSTALS HAVING OH GROUPS, K SUGIYAMA, K KATO, K SHIRAISHI, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 64(5), 1652 - 1657, May 1991
    Summary:Four kinds of ferroelectric liquid crystals (FLCs) possessing OH groups, ((S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 4a, (S)-2-methylbutyl 4-[4-(11-hydroxyundecyloxy)2-hydroxybenzylideneamino]cinnamate 4b, (S)-2-hydroxypropyl 4-[4-(11-hydroxyundecyloxy)benzylideneamino]cinnamate 4c, and (S)-2-hydroxypropyl 4-[4-(11-hydroxyundecyloxy)-2-hydroxybenzylideneamino]cinnamate 4d) were prepared in order to understand how the introduction of OH groups in FLCs affect the phase behavior and electrooptical effects. Alpha,omega-Diols 4c and 4d show a higher transition temperature of the smectic A (S(A)) phase than do omega-hydroxyl compounds 4a and 4b. Comparing 4b and 4d both of which have an OH group at the omicron-position of the benzylidene group with 4a and 4c, the former samples show a higher S(A)-isotropization (I) transition temperature than do the latter ones. It is suggested from IR spectroscopic results that inter- and intramolecular hydrogen bonding contribute to the stabilization of the S(A) phase. The order of the helical pitch in the chiral smectic C (S(c)*) phase was found to be 4b > 4a > 4d > 4c within 1.8-6.6-mu-m. From an electrooptical effect due to a deformation of the helical structure in the S(c) phase, the rise time of the surface director (tau-sr) and the rise time of the bulk director (tau-br) were measured to be as follows: tau-sr are 850, 1500, 30, and 70-mu-s, and tau-br are 12, 100, 0.17, and 0.35 ms for 4a, 4b, 4c, and 4d, respectively. It was found that the intramolecular hydrogen bonding results in a slower optical response time, whereas intermolecular hydrogen bonding results in a faster one.
  • ELECTROOPTICAL EFFECT OF ALPHA-OLEFINIC SMECTIC LIQUID-CRYSTALS, K SUGIYAMA, Y MORIKAWA, K SHIRAISHI, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (3), 230 - 234, Mar. 1991 , Refereed
    Summary:Three kinds of alpha-olefinic liquid crystals, 4-[4-[(S)-2-methylbutoxycarbonyl]phenoxycarbonyl]phenyl 10-undecenoate 1, 4-[4-[(S)-2-methylbutoxycarbonyl)]phenyl] phenyl 10-undecenoate 2, and (S)-2-methylbutyl 4-[N-4-(10-undecenoyloxy)benzylideneamino] cinnamate 3 were synthesized as reactive monomers for ferroelectric liquid crystalline comb-like polysiloxanes. 1 shows a smectic A(S(A)) state, while 2 and 3 show both S(A) and chiral smectic C (S(C)(*)) state. From the electrooptical measurements, the response times for dual frequency drive in S(A) state were found to be 30, 310, and 30 ms at 0.1 Hz : 2. 12, 7, and 2.67 s at 1 kHz for 1, 2 and 3, respectively. The response time for SSFLC in S(C)(*) phase was also found to be 460 and 105-mu-s for 2 and 3, respectively.
  • THIN-FILMS PLASMA POLYMERIZED FROM PROPYLENE-OXIDE AND CARBON-DIOXIDE MIXTURE, K SUGIYAMA, K SHIRAISHI, T IHARA, M KIBOKU, POLYMER JOURNAL, POLYMER JOURNAL, 23(10), 1287 - 1290, 1991 , Refereed
  • DETERMINATION OF ABSOLUTE RATE CONSTANTS FOR RADICAL POLYMERIZATION OF DIISOPROPYL FUMARATE BASED ON A QUANTITATIVE SCAVENGE OF PROPAGATING RADICAL, B YAMADA, E YOSHIKAWA, K SHIRAISHI, H MIURA, T OTSU, POLYMER, POLYMER, 32(10), 1892 - 1896, 1991
    Summary:During polymerization of diisopropyl fumarate (DiPF), the propagating poly(DiPF) radical was scavenged quantitatively by a stable free radical, because of its long-lived nature and high steady-state concentration. By addition of a known amount of 1,3,5-triphenylverdazyl to the polymerization mixture, the concentration of poly(DiPF) radical was determined to be 10(-5)-10(-4) mol dm-3, which allowed the evaluation of the propagation rate constant (k(p)) from the overall rate of polymerization: k(p) = 0.31 +/- 0.07 dm3 mol-1 S-1 at 30-degrees-C. The lifetime of the polymer radical was too long for a rotating sector experiment. The rate constant for bimolecular termination of the radical (k(t)) was calculated from the decay of poly(DiPF) radical followed by the scavenger method: k(t) = 0.84 dm3 mol-1 S-1. These rate constants, which are exceptionally small, are accounted for by the steric factor of the ester alkyl group accumulated along the polymer chain and extremely slow diffusion of the polymer radical. Because the termination rate determined is too slow to interpret the molecular weight of the polymer formed, primary radical termination is to be considered as an additional termination process in the actual polymerization.
  • LIQUID-CRYSTALLINE POLYMERS FROM FUMARATES CONTAINING MESOGENIC METHOXYPHENYLAZOPHENOXY GROUPS, K SHIRAISHI, K SUGIYAMA, CHEMISTRY LETTERS, CHEMISTRY LETTERS, (9), 1697 - 1700, Sep. 1990
  • SYNTHESIS AND PROPERTIES OF OLIGOMER CARRYING 2-SIGMA-BUTYL-4,6-DINITROPHENYL GROUP IN THE SIDE-CHAIN, K SUGIYAMA, K SHIRAISHI, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (8), 876 - 879, Aug. 1990 , Refereed
  • SYNTHESIS AND ELECTROOPTICAL PROPERTIES OF TERMINAL-TERMINAL TYPE OF FERROELECTRIC LIQUID-CRYSTAL, K SHIRAISHI, K KATO, K SUGIYAMA, CHEMISTRY LETTERS, CHEMISTRY LETTERS, (6), 971 - 974, Jun. 1990 , Refereed
  • SYNTHESIS AND PROPERTIES OF MACROMONOMERS CARRYING PHOSPHATIDYLCHOLINE ANALOGOUS AND AZOBENZENE MOIETIES, K SUGIYAMA, K SHIRAISHI, MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, 190(10), 2381 - 2390, Oct. 1989 , Refereed
  • PREPARATION AND PROPERTIES OF POLYMERIZABLE FERROELECTRIC LIQUID-CRYSTALLINE ACRYLATE AND METHACRYLATE DERIVATIVES, K SHIRAISHI, K SUGIYAMA, MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, 190(9), 2235 - 2243, Sep. 1989 , Refereed
  • EFFECT OF ALKYL GROUP ON THE LIQUID-CRYSTALLINE BEHAVIOR OF FUMARIC DERIVATIVES, K SHIRAISHI, A SAKAMOTO, K SUGIYAMA, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (6), 946 - 951, Jun. 1988 , Refereed
  • NEMATIC FUMARIC ESTER DERIVATIVES AND THEIR ELECTRO-OPTICAL PROPERTIES, K SHIRAISHI, K SUGIYAMA, A SAKAMOTO, T OTSU, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 61(3), 783 - 786, Mar. 1988 , Refereed
  • POLYMERIC DERIVATIVES OF CHLORAMPHENICOL AND THEIR ANTIBACTERIAL PROPERTIES, K SHIRAISHI, K SUGIYAMA, JOURNAL OF MACROMOLECULAR SCIENCE-CHEMISTRY, JOURNAL OF MACROMOLECULAR SCIENCE-CHEMISTRY, A25(8), 1015 - 1025, 1988 , Refereed
  • PREPARATION OF LIQUID-CRYSTALLINE FUMARIC ESTER DERIVATIVES AND THEIR THERMAL AND ELECTROOPTICAL PROPERTIES, K SUGIYAMA, A SAKAMOTO, Y MURATA, K SHIRAISHI, T OTSU, NIPPON KAGAKU KAISHI, NIPPON KAGAKU KAISHI, (6), 1064 - 1070, Jun. 1987 , Refereed
  • POLYMERS FROM 1,2-DISUBSTITUTED ETHYLENIC MONOMERS .11. RADICAL HIGH POLYMERIZATION OF DI-TERT-BUTYL FUMARATE AND NOVEL SYNTHESIS OF HIGH MOLECULAR-WEIGHT POLY(FUMARIC ACID) FROM ITS POLYMER, T OTSU, T YASUHARA, K SHIRAISHI, S MORI, POLYMER BULLETIN, POLYMER BULLETIN, 12(5), 449 - 456, 1984 , Refereed

Books etc

  • The 2nd International School of advanced plasma technology, Villa Monastero, Varenna, Italy, Biological characterization of nano carbon coating or/and biocompatible polymer grafting commodity materials for implantable medical devices by low temperature pl, Joint author,   2004 10

Conference Activities & Talks

  • Preparation of Randomcopolymers with poly(acrylamide) having L-Lysine Moiety and 2-(Methacryloyloxy)ethyl Phosphorylcholine and its Effect on Fibrinoltic Activity, Yuto OKI, Kohei SHIRAISHI,   2019 12 08
  • Preparation of Star-shaped L-lysine Acrylamide Polymer and Its Influence on Fibrinolytic Enzyme Reaction., Yuki TAKEBE, Kohei SHIRAISHI, Yuto OKI,   2019 12 08
  • Evaluation of wettability and swelling behaviors of zwitterionic polymers using non-contact wettability evaluation system, Risa Katayama, Chie Kojima, Nobuyuki Tanaka, Yo Tanaa, Kohei Shiraishi, Akikazu, Matsumoto,   2019 11
  • Preparation of poly[(meth)acrylamide]having L-lysine Moiety and its Effect on Fibrinolytic Activity, SHIRAISHI Kohei, ICBZM2017,   2017 10
  • Adhesion Properties of MPC Copolymer to Substrates and Cells, Chie kojima, Musashi Ikeda, Akikazu Matsumoto, Kenji Kajiyama, Tohru Sakoda, Kohei SHIRAISHI, IUMRS-ICAM2017,   2017 08
  • Evaluation of Adhesive Properties and Cell Adhesion of MPC Polymer-Coated Substrates, Musashi Ikeda, Chie kojima, kenji Kajiyama, Tohru Sakoda, Kohei Shiraishi, Akikazu Matsumoto, ICBS,   2016 11 28
  • Preparation Of A UCST Type Of Thermoresponsive Sulfobetaine Polymer Modified Poly(ethylene terephthalate)Films By Ar Plasma post Polymerization Method, Akinori IMAJO, Taiji ITO, SHIRAISHI Kohei, The 10th Asian-Europeean International Conference on Plasma Surface Engineering 2015,   2015 09
  • Preparation of UCST types of thermoresponsive polymer immobilized on a glass substrate by SI-RAFT polymerization method and thermal stimuli-exfoliation of hiMSC,   2015 05
  • Preparation of cell microarrays immobilized with protein binding sites and PEG segments on the glass spots and cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells, Akinori Imajo, Yuka Kanzaki, Kohei Shiraishi,   2015 05
  • Preparation of cell microarray immobilized with various thermosensitive polymers on the glass spots and evaluation of cell exfoliation by thermal stimulation on their substrates, Tatsuki Nousou, Akinori Imajo, Yuka Kanzaki, Yasue Yamada, Kohei Shiraishi, Hirofumi Kawazumi,   2014 09
  • Highly efficient cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells by using cell microarrays immobilized with PEG on the glass spots, Akinori Imajo, Yuka Kanzaki, Yasue Yamada, Kohei Shiraishi,   2014 09
  • Preparation of PNiPAAm immobilized polyacetylene films by plasma irradiation-post polymerization and cell attachment, proliferation, and thermal-stimuli exfoliation on their films, Tatsuki Nousou, Yasue Yamada, Kohei Shiraishi,   2014 05
  • Preparation of PNiPAAm with various chain lengths immobilized glass spots on a microarray by SI-ATRP method and cell attachment and thermal stimuli-exfoliation of cells on the microarray, Akinori Imajo, Tatsuki Nousou, Yuka Kanzaki, Yasue Yamada, Kohei Shiraishi, Tatsuyuki Nakatani,   2014 05
  • Preparation of PNiPAAm with various polymer chain lengths immobilized on a glass substrate by SI-ATRP method and cell attachment and thermal stimuli-exfoliation of mesenchymal stem cell on the substrate, Akinori Imajo, Manato Asai, Yasue Yamada, Kohei Shiraishi, Tatsuyuki Nakatani,   2013 09
  • Preparation of UCST type of poly(acrylamide-co-acrylonitrile) immobilized on a glass substrate by SI-ATRP method and heat-cell exfoliation on the substrate, Taiji Ito, Nousou Tatuki, Yasue Yamada, Kohei Shiraishi, Hirofumi Kawazumi, Tatsuyuki Nakatani,   2013 09
  • Improvement of Physicochemical Properties of Natural Rubber Reinforced with Mechanical nanofibrillated cellulose, Hirotaka Mita, Toru Yano, Kohei Shiraishi, Takashi Endo,   2013 09
  • Preparation of surface-modified substrates with UCST type of thermosensitive polymers and evaluation of cell exfoliation by thermal stimulation on their substrates, Taiji Ito, Tatsuki Nousou, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Hirofumi Kawazumi, Tatsuyuki Nakatani,   2013 05
  • Highly efficient cell fusion of microcells with human artificial chromosomes(HACs) as gene-delivery vectors and U937 cells by using cell microarrays, Akinori Imajo, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani,   2013 05
  • Preparation of surface modified substrates with UCST or LCST types of thermosensitive polymer and evaluation of cell exfoliation on their surfaces by thermal stimulation or laser light irradiation, Manato Asai, Taiji Ito, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Hirofumi Kawazumi, Tatsuyuki Nakatani,   2012 09
  • Cell fusion of microcells with Human Artificial Chromosome(HAC) and white blood cells(U937) on microarray spots grafted PMPC with cell specific antibodies, Akinori Imajo, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani,   2012 09
  • Preparation of surface-modified substrates with UCST or LCST types of thermosensitive polymer and evaluation of cell attachment and exfoliation on their substrates, Taiji Ito, Manato Asai, Tatsuya Ishihara, Yasue Yamada, Kohei Shiraishi, Sugiyama Kazuo, Hirofumi Kawazumi, Tatsuyuki Nakatani,   2012 05
  • Preparation of proteinA/G carrying poly(MPC) spotted microarray and evaluation of cell-specific attachment on the substrate, Akinori Imajo, Akihiro Tominaga, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Hirofumi Kawazumi, Tatsuyuki Nakatani,   2012 05
  • Preparation of Fluorinated Polyurethanes Having an Antifouling Ability, Tatsuya Kawakami, Kohei Siraisi, Kazuo Sugiyama, Naokatu Hisanaga,   2011 09
  • Preparation of thermoresponsive polymer-immobilized microarrays for cytoscreening and cell recovery, Akinori Imajo, Kazuo Sugiyama, Kohei Shiraishi, Yasue Yamada, Hirofumi Kawazumi, Tatsuyuki Nakatani, Keishi Okamoto, Yuki Nitta,   2011 09
  • Preparation of surface-modified substrates with anti-body carrying poly(MPC) and evaluation of cell specific attachment on their surfaces, Akihiro Tominaga, Akinori Imajo, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama,   2011 09
  • Development of system cell diagnosis and removal of unnecessary cells by using visible laser light irradiating to cell microarray, Tatyuya Ishihara, Ryota Kobayashi, Manato Asai, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiya, Hirofumi Kawazumi, Tatsuyuki Nakatani, Keishi Okamoto, Yuki Nitta,   2011 09
  • Preparation and characterization of anti-freezing paint by using a polyion complex, Ryohei Shimoji, Kohei Shiraishi, Kazuo Sugiyama,   2011 05
  • Preparation of cell microarray modified with poly(MPC) and/or thermosensitive polymer for cell diagnosis and acquisition of targeting cell by using visible light laser, Tatsuya Ishihara, Ryota Kobayashi, Manato Asai, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Hirofumi Kawazumi, Tatsuyuki Nakatani, Keishi Okamoto,   2011 05
  • Preparation of substrates surface-modified with anti-body binding poly(MPC) and evaluation of cell specific attachment on their surfaces, Akihiro Tominaga, Akinori Imajo, Keita Yamasaki, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama,   2011 05
  • Preparation of poly (L-lactic acid) / natural rubber composites reinforced with epoxidized natural rubber and polycarbodiimide for improvement of the thermal and impact mechanical properties, Shusaku Sagara, Dai Harada, Nozomu Tchiyoda, Keiko Sakaguchi, Toru Yano, Kohei Shiraishi, Kazuo Sugiyama,   2010 09
  • Preparation of MPC polymer having antibody binding sites for the isolation of targeting cells using a single cell-microarray and immobilization of its polymer on various substrates, Ryota Kobayashi, Akihiro Tominaga, Tatsuya Ishihara, Akinori Imajo, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2010 09
  • Preparation of thermosensitive surface with PNiPAAm and PMPC for fabrication of a cell microarray and thermal-stimuli detachment of cells on its surface, Ryota Kobayashi, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2010 05
  • Development of novel anti-freezing paint by using polyioncomplex, Ryohei Shimoji, Yoshiaki Yanagioka, Kohei Shiraishi, Kazuo Sugiyama,   2010 05
  • Effect of the addition of poly(carbodiimide) as a hydrolysis retarder on thermal and mechanical properties of poly (L-lactic acid) composite consisting of natural rubber and epoxidized natural rubber, Dai Harada, Shusaku Sagara, Toru Yano, Keiko Sakaguchi, Kunihiko Hashimoto, Kohei Shiraishi, Kazuo Sugiyama,   2010 05
  • Preparation of poly (L-lactic acid) composites reinforced with micro-phase separated natural rubber containing epoxidized natural rubber for improvement of their impact mechanical properties, Shusaku Sagara, Dai Harada, Toru Yano, Keiko Sakaguchi, Kohei Shiraishi, Kazuo Sugiyama,   2009 09
  • Preparation of cell microarray with single-cell attachment and detachment from thermo-responsible polymer surface, Ryota Kobayashi, Yasunori Yone, Tatsuyuki Nakatani, Keishi Okamoto, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama,   2009 09
  • Surface modification with biocompatible polymers onto functional group containing carbon thin film coating substrate by Ar plasma treatment post/graft polymerization technique or condensation with COOH or NH2 terminated polymer and characterization of the, Keita Yamasaki, Ryoji Hamawaki, Tatsuyuki Nakatani, Keishi Okamoto, Yuki Nitta, Kohei Shiraishi, Kazuo Sugiyama,   2009 09
  • Preparation of natural rubber / poly (L-lactic acid) composite reinforced with epoxidized natural rubber for improvement of thermal and mechanical properties of poly(L-lactic acid), Shusaku Sagara, Dai Harada, Toru Yano, Keiko Sakaguchi, Kohei Shiraishi, Kazuo Sugiyama,   2009 05
  • Preparation of cell microarrays with both thermosensitive poly(HPMA-co-MMA) as a cell attachment domain and PMPC as a cell non-attachment domain, and behavior of cell attachment or thermal-stimuli detachment on its surface, Yasunori Yone, Ryota Kobayashi, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2009 05
  • Surface modification with biocompatible polymers onto nitrogen containing carbon thin film coated aluminum substrate by Ar plasma treatment post/graft polymerization technique or condensation with carboxylic acid terminated polymer and characterization, Ryouji Hamawaki, Keita Yamasaki, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto, Yuki Nitta,   2009 05
  • Improvement of mechanical and thermal properties of poly (L-lactic acid) by compounding epoxidized natural rubber, Shusaku Sagara, Ken Ishibashi, Toru Yano, Keiko Sakaguchi, Kohei Shiraishi, Kazuo Sugiyama,   2008 09
  • Preparation of New Hydrogel Polyurethane Films based on Hyper Branched Polymer and their Application to Anti-Fouling Paint., Akihito Yokoyama, Kohei Shiraisi, Kazuo Sugiyama, Koji Ohga,   2008 09
  • Development of biocompatible hydrogel polymer with UV absorber and their characterization, Kota Yamasaki, Miho Hirano, Kohei Shiraishi, Kazuo Sugiyama,   2008 09
  • Preparation, cell attachment and thermal exfoliation on the surface of cell array patterned with thermosensitive poly(N-2-hydroxypropyl- methacrylamide- co-methyl methacrylate) and PMPC, Yasunori Yone, Yumi Kanakura, Takuji Yamamoto, Tatsuyuki Nakatani, Keishi Okamoto, Yasue Yamada, Kohei Shiraishi, Kazuo Sugiyama,   2008 09
  • Chemical modification of thin carbon coating metallic surfaces with functional groups and blood compatibility, Ryouji Hamawaki, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2008 09
  • Chemical modification of thin carbon coating metallic surfaces with functional groups and their surface characterization, Ryouji Hamawaki, Kouhei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2008 05
  • Cell attachment on cell array modified with thermosensitive polymer and thermal stimuli-cell exfoliation on the surface, Yasunori Yone, Yumi Kanakura, Takuji Yamamoto, Tatsuyuki Nakatani, Keishi Okamoto, Kohei Shiraishi, Kazuo Sugiyama,   2008 05
  • Improvement of mechanical and thermal properties of poly (L-lactic acid) by compounding natural rubber and their compatibilizer, Shusaku Sagara, Ken Ishibashi, Keiko Sakaguchi, Toru Yano, Kohei Shiraishi, Kazuo Sugiyama,   2008 05
  • Characterization of surface-modified thermoresponsible polymer on PET film by the analysis of peptides bound to their surfaces using bacterial random peptide library method, Yusuke Kojitani, Tetsuyuki Shigesada, Kohei Shiraishi, Kazuo Sugiyama, Kazuhiko Yamamoto,   2007 09
  • Immobilization of hydrophilic polymer on thin carbon coating surface with different ratio of sp2/sp3 carbon hybridization by plasma irradiation-post polymerization technique, Ryouji Hamawaki, Tatsuyuki Nakatani, Keishi Okamoto, Kohei Shiraishi, Kazuo Sugiyama,   2007 09
  • Preparation of cell microarray surface-modified with thermoresponsible polymer and polyethyleneglycol for preventing cell attachment, Yumi Kanakura, Tatsuyuki Nakatani, Keishi Okamoto, Kohei Shiraishi, Kazuo Sugiyama, Yasunori Yone,   2007 09
  • Preparation of block copolymer consisting of poly(D-lactic acid) segment having hydrophilic groups as a crystallizer and propyleneglycol / succinic acid copolymer segment as a compatibilizer and improvement of mechanical and thermal properties of, Ken Ishibashi, Yukio Kouzai, Syuusaku Sagara, Kohei Shiraishi, Kazuo Sugiyama, Tooru Yano,   2007 09
  • Evaluation of surface properties of PET film modified with thermoresponsible poly(N-2-hydroxypropylmethacrylamide-co-alkyl methacrylate) copolymer using bacterial random peptide library method, Yusuke Kojitani, Tetsuyuki Shigesada, Tatsuya Iguchi, Kazuhiko Yamamoto, Kohei Shiraishi, Kazuo Sugiyama,   2007 05
  • Preparation of cell array surface-modified with thermoresponsible poly(N-2-hydroxypropyl, Yumi Kanakura, Yasunori Yone, Tatsuyuki Nakatani, Keishi Okamoto, Kohei Shiraishi, Kazuo Sugiyama,   2007 05
  • Immobilization of hydrophilic polymer on thin carbon coating surface by plasma treatment-post polymerization technique, Maki Nishioka, Ryouzi Hamawaki, Shingo Wakisaka, Kohei Shiraishi, Kazuo Sugiyama, Tatsuyuki Nakatani, Keishi Okamoto,   2007 05
  • Molecular assembly and blood compatibility of amphiphilic block copolymers, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama,   2007 05
  • Preparation of poly (D-lactic acid) introduced with hydrophilic moieties as a novel crystallizer for improvement of heat and impact properties of poly (L-lactic acid), Ken Ishibashi, Yukio Kouzai, Tooru Yano, Kohei Shiraishi, Kazuo Sugiyama,   2007 05
  • Preparation of block copolymer with branched poly(D-lactic acid) as crystallizing segment and improvement of mechanical and thermal properties of poly(L-lactic acid), yukio kouzai, ken isibasi, tooru yano, kohei siraisi, kazuo sugiyama,   2006 09
  • Preparation of vinylcopolymer with zwitterionic L-Lysine as fibrinolytic factor binding sites and phophatidylcholine moieties and inner coating in a narrow tube by a nonthermal plasma flow at atmospheric pressure, Shingo Wakisaka, Tatsuyuki Nakatani, Keishi Okamoto, Kohei Shiraishi, Kazuo Sugiyama,   2006 09
  • Biological characterization of polymer material surface by bacterial random peptide library method, Tetsuyuki Shigesada, Yusuke Kojitani, Kazuhiko Yamamoto, Kohei Shiraishi, Kazuo Sugiyama,   2006 09
  • Preparation of block copolymers composed of hydrophilic and hydrophobic segment using novel polydimethylsiloxane type polymer azo-initiators, Nobuyuki Tanigawa, Kohei Shiraishi, Kazuo Sugiyama,   2006 09
  • Development of moldable porous hydroxyapatite by using hybrid polymer microsphere, takayuki imoto, yuka senda, kohei shiraishi, kazuo sugiyama,   2006 09
  • Preparation of novel block copolymer with poly(L-lactic acid) crystallizing segment and improvement of mechanical and thermal properties of poly(L-lactic acid), yukio kouzai, tooru yano, kohei shiraishi, kazuo sugiyama,   2006 05
  • Preparation of porous hydroxyapatite by using surface modified polymethylmethacrylate microsphere with phosphoric acid moiety, takayuki imoto, yuka senda, kohei shiraishi, kazuo sugiyama,   2006 05
  • Preparation of copolymer with zwitterionic L-Lysine as plasminogen binding sites and phophatidyl choline moieties and its effect on fibrinolytic activity, shingo wakisaka, Kohei Shiraishi, kazuo sugiyama,   2006 05
  • Biological characterization of thermoresponsible polymer grafted PET surface by bacterial random peptide library method, Tetsuyuki Shigesada, Yusuke Kojitani, Kazuhiko Yamamoto, Kohei Shiraishi, Kazuo Sugiyama,   2006 05
  • Evaluation of adsorption of serum proteins onto films immobilized with poly(meth)acrylamide having zwitterionic L-lysine moiety by using antibody against their proteins and that of their antithrombogenicity, Satozaki Jyunji, Yamamoto Kazuhiko, Shiraishi Kohei, Sugiyama Kazuo,   2005 09
  • Biological characterization of thermoresponsible polymer grafted PET by bacterial random peptide library method(FliTrx), Shigesada Tetsuyuki, Yamamoto Kazuhiko, Shiraishi Kohei, Sugiyama Kazuo,   2005 09
  • Proliferation and thermal exfoliation of HeLa cells on thermoresponsible poly(2-hydroxypropylmethacrylamide-co-MMA) grafted film surfacce with cell attachment peptide RGDS, Maeda Kazuma, Shiraishi Kohei, Sugiyama Kazuo,   2005 09
  • Preparation of poly[(meth)acrylamide] containing L-lysine moiety and its effect of fibrinolytic activity, Wakisaka Shingo, Oga Koji, Shiraishi Kohei, Sugiyama Kazuo,   2005 09
  • Graft polymerization of methacrylamide modified with L-lysine moieties and evaluation of antithrombogenic properties on various materials, The 8th SPSJ International Polymer Conference(IPC2005),   2005 07 , The 8th SPSJ International Polymer Conference(IPC2005)
  • Preparation of poly(N-isopropylacrylamide)/n-butylmethacrylate IPN gel consisting of amphoteric D- or L- serine and characterization of its gel, ando akira, Nishioka Maki, Shiraishi Kohei, Sugiyama kazuo,   2005 05
  • Biological Characterization of thermoresponsible poly(2-hydroxypropylmethacrylamide) grafted PET by bacterial random peptide library method(FliTrxTM), sigesada tetsuyuki, nakayama syuji, Yamamoto Kazuhiko, Shiraishi Kohei, Sugiyama Kazuo,   2005 05
  • Effect of HeLa cell attachment and proliferation on cell attachment peptide / biocompatible polymer immobilized film, Maeda Kazuma, Hirano Miho, Mishima Kanako, Shiraishi Kohei, Sugiyama Kazuo,   2005 05
  • Evaluation of the adsorption of fibrinolytic proteins on poly(Nalpha-methacrylamide-L-lysine) immobilized polymer film by using antibody against the proteins and antithrombogenicity of its film, Satozaki Jyunji, Wakisaka Shingo, Yamamoto Kazuhiko, Shiraishi Kohei, Sugiyama Kazuo,   2005 05
  • Preparation of novel poly(Na-acrylamide-L-lysine) and interaction between its polymer and fibrinolytic proteins, Yanase Shogo, Wakisaka Shingo, Shiraishi Kohei, Sugiyama Kazuo,   2005 05
  • Preparation of thermo- and lightsensitive poly(2-hydroxypropylmethacrylamide) grafted PET film as a material for cell culture-dish, ODAN MEGUMI, TUJIMOTO MASASI, MAEDA KAZUMA, SIRAISI KOUHEI, SUGIYAMA KAZUO,   2004 09
  • Preparation of polymethacrylamide having L-lysine residue in the side chain with fibrinolytic activity and evaluation of the biocompatibility, Kohta Masushi, Satozaki Jyunji, Shiraishi Kohei, Sugiyama Kazuo,   2004 09
  • Preparation of poly(O-methacryloyl-L-serine-co-methacrylic acid) having anticoagulant activity and evaluation of the biocompatibility, Asami Gou, Satozaki Jyunji, Shiraishi Kohei, Sugiyama Kazuo,   2004 09
  • Preparation of poly[O-methacryloyl-L-Serine] by anionic polymerization and characterization of its polymer, SATOZAKI Jyunji, ASAMI Gou, SHIRAISHI Kohei, SUGIYAMA Kazuo,   2004 05
  • Effect of pH and adding salt on lower critical solution temperature of copolymer consisting N-isopropylacrylamide and O-methacryloyl-L-serine, HARUHARA Yoshihiro, WAKISAKA Shingo, SHIRAISHI Kohei, SUGIYAMA Kazuo,   2004 05
  • Proliferation and exfoliation of HeLa cells on thermo and light sensitive poly(N-2- hydroxypropylmethacrylamide) grafted PET, MAEDA Kazuma, OHDAN Megumi, TUJIMOTO Masashi, SHIRAISHI Kohei, SUGIYAMA Kazuo,   2004 05
  • Preparation of pH responsible gel membrane containing amphoteric amino acid and effect of adding L-amino acid on the membrane potentials, ANDO Akira, SHIRAISHI Kohei, SUGIYAMA Kazuo,   2004 05
  • Interaction of polymethacrylamides having L-lysine residue in the side chain with serum protein and evaluation of their fibrinolytic activity, KOHTA Masushi, SATOZAKI Jyunji, SHIRAISHI Kohei, SUGIYAMA Kazuo,   2004 05
  • Effect of structure of alkyl group on the phase transition of aqueous poly (N isopropylacrylamide co alkylacrylamide) solution,   2001 05

Misc

  • Development of hybrid materials based on plant-derived poly(lL-actic acid) and natural rubber for industrial uses, SHIRAISHI Kohei, GSCN News Letter, 33, 3,   2009
  • Recent some approaches for development of new soft materials based on mimicing functionalized biomolecules,   2001 01
  • Preparation of moleculary imprinted polymer with transition state analogue and effect of polymer structure on enzyme-mimics catalytic activity, Research reports of the School of Engineering Kinki University, 34, 9, 15,   2000
  • TERMINAL-TERMINAL TYPES OF LIQUID-CRYSTALS .1. SYNTHESIS AND ELECTROOPTICAL PROPERTIES OF A FERROELECTRIC LIQUID-CRYSTAL, K SHIRAISHI, K KATO, K SUGIYAMA, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 63, 6, 1848, 1850,   1990 06
  • LIQUID-CRYSTALLINE POLYMERS PREPARED BY POLYADDITION OF DITHIOLS TO MESOGENIC BISACRYLATES OR BISMETHACRYLATES, K SUGIYAMA, K SHIRAISHI, JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 28, 7, 1983, 1988,   1990 06
  • MONOMER-ISOMERIZATION RADICAL POLYMERIZATION OF DI-TERT-BUTYL MALEATE TO HIGH MOLECULAR-WEIGHT POLY(DI-TERT-BUTYL FUMARATE), T OTSU, K SHIRAISHI, MACROMOLECULES, 18, 9, 1795, 1796,   1985

Research Grants & Projects

  • Preparation of Biocompatible polymer
  • Preparation and properties of novel side-chain liquid crystalline polymers
  • Preparation and properties of functionalized polymer microspheres

Educational Activities

Teaching Experience

  • Advanced Lecture on Biomaterial Chemistry, Graduate School of Systems Engineering, Kinki University
  • Polymer Chemistry, Faculty of Engineering, Kinki University

社会貢献活動情報

Social Contribution

  • Development of polymer biomaterials for practical application of pluripotent stem cell, Lecturer,   2014 08 21
  • Preparation of novel cell operatiing system for regenerative medicine, Lecturer,   2013 01 23