KINDAI UNIVERSITY


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ISHIWATA Shunji

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FacultyDepartment of Pharmacy / Graduate School of Medicine
PositionAssociate Professor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/767-ishiwata-shunji.html
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Last Updated :2020/09/30

Research Activities

Research Areas

  • Life sciences, Clinical pharmacy

Published Papers

  • Investigation of the levels of Ifosfamide vaporized from powder and solution, ISHIWATA Shunji, Japanese Journal of Occupational Medicine and Traumatology, Japanese Journal of Occupational Medicine and Traumatology, 67, 95 - 99, 2019 , Refereed
  • Development of a specific control system for drug logistics in disaster-hit regions, Shunji Ishiwata, Manabu Kitakoji, Tomomi Inoue, Tohru Ohtori, Takeshi Kotake, Japanese Journal of Occupational Medicine and Traumatology, Japanese Journal of Occupational Medicine and Traumatology, 66, 156 - 163, 2018 , Refereed
  • Decomposition of anthracycline anticancer drugs by ozone gas as a decontaminant, ISHIWATA Shunji, Jouranl of Medical and Hyienic Use of Ozone, Jouranl of Medical and Hyienic Use of Ozone, 25, 90 - 97, 2018 , Refereed
  • Evaluation of BLS skills by a BLS training program for pharmacy students -activation of the EMS system, rescue breathing, and AED operation, Tomomi Inoue, Shuho Yaegashi, Ryuzo Kyukawa, Shunji Ishiwata, Hiroshi Nonogi, Takeshi Kotake, Journal of Clinical Simulation Research, Journal of Clinical Simulation Research, 7, 8 - 14, 2017 , Refereed
  • Improvement and limitation of chest compression skills by a BLS training program, Tomomi Inoue, Shuho Yaegashi, Ryuzo Kyukawa, Shunji Ishiwata, Hiroshi Nonogi, Takeshi Kotake, Journal of Clinical Simulation Research, Journal of Clinical Simulation Research, 7, 19 - 24, 2017 , Refereed
  • A Survey of Environmental Radioactive Cesium Due to the Accident of TEPCO Fukushima Daiichi NPP in Kawamata-machi, Fukushima-ken, YAMANISHI Hirokuni, HORIBATA Akira, SUZUKI Takahiro, FURUKAWA Michio, INAGAKI Masayo, WAKABAYASHI Genichiro, HOHARA Sin-ya, ITOH Tetsuo, TANAKA Naomichi, ISHIWATA Shunji, TAGA Atsushi, OGATA Fumihiko, Journal of Smart Processing, Journal of Smart Processing, 4(6), 268 - 274, 2015 , Refereed
  • 99%以上のセシウムを除去するゼオCa漆喰の開発および適用施設例, MORIMURA TSUYOSHI, TAGA ATSUSHI, ISHIWATA SHUNJI, OGATA FUMIHIKO, 機能材料, 機能材料, 32(12), 50 - 57, Nov. 05 2012
  • 核内核外輸送を介したRNA結合蛋白質Rnc1の制御機構, SATO RYOSUKE, MATSUMURA YASUHIRO, UMEDA NANAE, TANAKA AKITOMO, TAKADA MAKOTO, KITA AYAKO, ISHIWATA SHUNJI, TAGA ATSUSHI, SUGIURA REIKO, 生化学, 生化学, ROMBUNNO.3T10P-11, 2011
  • Molecular genetic approach to identify inhibitors of signal transduction pathways: Fission yeast as a model system for drug discovery, Shunji Ishiwata, Takayoshi Kuno, Hirofumi Takada, Atsushi Koike, Reiko Sugiura, Reiko Sugiura, Source Book of Models for Biomedical Research, Source Book of Models for Biomedical Research, 439 - 443, Dec. 01 2008
    Summary:The fission yeast Schizosaccharomyces pombe (S. pombe) has become a valuable model system to elucidate the mechanisms of basic cellular functions of higher eukaryotes, including cell cycle control, membrane trafficking, and signal transduction. Having the smallest genome size among eukaryotes and with its powerful genetics, this organism is also an excellent model system for drug discovery. In addition, many signaling molecules targeted by the drug or the homologues of disease-linked human genes are highly conserved. We have been studying the signal transduction pathway in fission yeast with special emphasis on calcineurin phosphatase and mitogen-activated protein kinase (MAPK) signal transduction pathways. Our molecular genetic approach, which utilizes a crosstalk between calcineurin and MAPK signaling, has identified several regulators of Pmk1 MAPK, which is a homologue of extracellular signal-regulated kinase (ERK) in mammals. As MAPK signal transduction pathways are one of the most attractive targets for cancer therapy, inhibitors that target this signaling appear to be promising drug candidates for the treatment of cancer. Here, we first give an overview of the use of yeast as a model system for drug discovery and then we introduce our molecular genetic strategy to identify regulators of MAPK signaling and the application of this approach to drug discovery. © 2008 Humana Press Inc.
  • RNA結合タンパク質Nrd1による細胞質分裂の制御機構, SATO RYOSUKE, MORITA TAKAHIRO, WATANABE SARA, TAKADA HIROFUMI, KITA AYAKO, ISHIWATA SHUNJI, KUNO TAKAYOSHI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 28P, Mar. 01 2008
  • アクチン結合タンパク質Cis3のMAPキナーゼ経路における働き, TAKAMURA AKIRA, ISHIWATA SHUNJI, KITA AYAKO, KUNO TAKAYOSHI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 27P, Mar. 01 2008
  • 分子遺伝学的手法によるPKCシグナル制御因子の同定と解析, DOI AKIRA, KITA AYAKO, AMAYA MISUZU, YOSHIMURA MIYA, YASUDA HITOKO, ISHIWATA SHUNJI, KUNO TAKAYOSHI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 27P, Mar. 01 2008
  • Cell integrityシグナルに関与するPmk1 MAPKの新たな標的分子の同定とその機能解析, TAKADA HIROFUMI, NISHIDA AIKO, KAI NAOSHI, ASAYAMA YUTA, SAKAI YOSHIHIRO, DOI AKIRA, MORIUCHI SAYAKO, KITA AYAKO, ISHIWATA SHUNJI, KUNO TAKAYOSHI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 27P, Mar. 01 2008
  • ストレス応答MAPKシグナルにおける内因性カルシニューリン抑制因子Cbp1の役割, NISHIDA AIKO, TAKADA HIROFUMI, KATSURA KOSAKU, KAI NAOSHI, KITA AYAKO, DOI AKIRA, ISHIWATA SHUNJI, KUNO TAKAYOSHI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 29P, Mar. 01 2008
  • 分裂酵母モデル生物を用いたクラスリンアダプター複合体と低分子量Gタンパク質Rho3の機能的関係, TAKESHIMA SAORI, TANAKA MARIKO, KITA AYAKO, TAKADA HIROFUMI, TONDA SHOKO, KITO SHOKO, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 131(3), 28P, Mar. 01 2008
  • 分裂酵母モデル生物を用いたPI(4)P5キナーゼを介する細胞内輸送経路の分子遺伝学的解析, KITA AYAKO, KAI NAOSHI, TAKADA HIROFUMI, FURUSONO HIROSHI, NISHIYAMA YUYA, IMAGAWA KIWAMU, KUNO TAKAYOSHI, DOI AKIRA, AMAYA MISUZU, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 129(2), 29P, Feb. 01 2007
  • ゲノム薬理学的アプローチによるMAPK経路の制御因子の同定:Geranyl geranyl transferaseは低分子量Gタンパク質Rhoを介してMAPKシグナルを制御する, MORIUCHI SAYAKO, ASAYAMA YUTA, KITA AYAKO, TAKADA HIROFUMI, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 129(2), 28P, Feb. 01 2007
  • 分裂酵母モデル系を用いたカルシニューリンの内因性インヒビターCbp1の細胞内シグナル伝達における働き, KATSURA KOSAKU, NISHIDA AIKO, TAKAMURA AKIRA, KITA AYAKO, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 129(2), 27P, Feb. 01 2007
  • 分裂酵母モデル生物を用いた免疫抑制薬感受性変異体の原因遺伝子its4+の細胞内輸送における働き, TAKADA HIROFUMI, YOROZUSE TAKAAKI, TONDA SHOKO, NISHIMURA MASAYUKI, KITA AYAKO, IMANAKA YUKAKO, TAKEUCHI MAI, KUNO TAKAYOSHI, ISHIWATARI SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 129(2), 28P, Feb. 01 2007
  • 分裂酵母モデル系を用いたクラスリンアダプター複合体の機能解析, TONDA SHOKO, TAKADA HIROFUMI, TANAKA MARIKO, TAKESHIMA SAORI, KITA AYAKO, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 129(2), 28P, Feb. 01 2007
  • 分裂酵母モデル生物を用いたクラスリンアダプター複合体と低分子量G蛋白質Rho3の機能的関係, TANAKA MARIKO, TAKESHIMA SAORI, KITA AYAKO, TAKADA HIROFUMI, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 128(2), 19P, Aug. 01 2006
  • 分裂酵母モデル生物を用いたRNA結合タンパク質を介する細胞骨格系制御のメカニズムの解析, MORITA TAKAHIRO, SATO RYOSUKE, TAKADA HIROFUMI, DOI AKIRA, KITA AYAKO, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 128(2), 19P, Aug. 01 2006
  • ゲノム薬理学的手法によるMAPキナーゼ経路において機能する因子の同定と機能解析, ASAYAMA YUTA, MORIUCHI SAYAKO, KITA AYAKO, INIWA SHIORI, IMANAKA YUKAKO, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 128(2), 19P, Aug. 01 2006
  • 分裂酵母モデル生物を用いたAP‐1複合体の細胞内輸送における役割とたんぱく質リン酸化による制御機構の解析, TAKADA HIROFUMI, TONDA SHOKO, KITA AYAKO, KATSURA KOSAKU, TANAKA MARIKO, TAKEUCHI MAI, KUNO TAKAYOSHI, ISHIWATA SHUNJI, SUGIURA REIKO, 日本薬理学雑誌, 日本薬理学雑誌, 128(2), 19P, Aug. 01 2006
  • Attribute Differences in Knowledge and Consciousness of Dementia by Hierarchical Clustering Analysis of the Questionnaire with Dementia Workshop for Pharmacy Workers, Daiki Iida, Norio Sakamoto, Kazuya Murata, Noriaki Nagai, Manabu Kitakouji,Toru Otori, Toshihiko Ishizaka, Shunji Ishiwata, Tomomi Inoue, Takeshi Kotake, The Journal of Community Pharmacy and Pharmaceutical Sciences, The Journal of Community Pharmacy and Pharmaceutical Sciences, 2019 , Refereed
  • Pancreatic cancer stem cells: features and detection methods., Toshiyuki Ishiwata, Yoko Matsuda, Hisashi Yoshimura, Norihiko Sasaki, Shunji Ishiwata, Naoshi Ishikawa, Kaiyo Takubo, Tomio Arai, Junko Aida, Pathology oncology research : POR, Pathology oncology research : POR, 24(4), 797 - 805, Oct. 2018 , Refereed
    Summary:Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with a high incidence of distant metastasis and recurrence. Cancer stem cells (CSCs), which are pluripotent, self-renewable, and capable of forming tumors, contribute to PDAC initiation and metastasis and are responsible for resistance to chemotherapy and radiation. Three types of experimental methods are commonly used to identify CSCs: CSC-specific marker detection, a sphere-formation assay that reveals cell proliferation under non-adherent conditions, and detection of side-population (SP) cells that possess high intracellular-to-extracellular pump functions. Several CSC-specific markers have been reported in PDACs, including CD133, CD24, CD44, CXCR4, EpCAM, ABCG2, c-Met, ALDH-1, and nestin. There remains controversy regarding which markers are specific to PDAC CSCs and which are expressed alone or in combination in CSCs. Examining characteristics of isolated CSCs and discovering CSC-specific treatment options are important to improve the prognosis of PDAC cases. This review summarizes CSC-detection methods for PDAC, including CSC-marker detection, the sphere-formation assay, and detection of SP cells.
  • Fibroblast growth factor receptor-4 (FGFR-4) as a novel therapeutic target for pancreatic cancer, Toshiyuki Ishiwata, Hisashi Yoshimura, Yoko Matsuda, Shunji Ishiwata, CANCER RESEARCH, CANCER RESEARCH, 76, Jul. 2016 , Refereed
  • Application of ozone gas for decontamination of nucleoside anticancer drugs., Ayumi Tsukamoto, Shunji Ishiwata, Asami Kajimoto, Ryusuke Murata, Rika Kitano, Tomomi Inoue, Takeshi Kotake, Journal of pharmaceutical health care and sciences, Journal of pharmaceutical health care and sciences, 2(26), 26 - 26, 2016 , Refereed
    Summary:BACKGROUND: Exposure to anticancer drugs is hazardous and may lead to chromosomal abnormalities and spontaneous abortion in healthcare workers. Guidelines recommend surface decontamination and cleaning in order to minimize the occupational exposure to anticancer drugs, although no single process has been found to deactivate all currently available hazardous drugs. Ozone gas is oxidative and a decontaminant for bacteria; its characteristic as a gas has advantages in that it does not need to be wiped off or neutralized after use. METHODS: The nucleoside anticancer drugs, cytarabine and fluorouracil, were exposed to ozone gas on plates under controlled humidity. The levels of exposed ozone were evaluated using the concentration-time (CT) value, which is the mathematical product of ozone concentration and exposure time. The effects of exposure to ozone on levels of the anticancer drugs were determined by high-performance liquid chromatography (HPLC). RESULTS: The levels of cytarabine decreased with increasing CT value and were not detected beyond 40,000 CT. The decomposition levels of the anticancer drug by ozone were CT-dependent irrespective of the maximum concentration of ozone. Higher humidity in the range from 70 to 90 % accelerated the decomposition of cytarabine and fluorouracil, and neither of the drugs were detected at 90 % humidity after exposure to ozone gas. CONCLUSIONS: Ozone gas decomposed these nucleoside anticancer drugs. This is the first report of the applicability of ozone gas as a decontaminator for anticancer drugs.
  • Role of the Small GTPase Rho3 in Golgi/Endosome trafficking through functional interaction with adaptin in Fission Yeast., Ayako Kita, Cuifang Li, Yang Yu, Nanae Umeda, Akira Doi, Mitsuko Yasuda, Shunji Ishiwata, Atsushi Taga, Yoshitaka Horiuchi, Reiko Sugiura, PloS one, PloS one, 6(2), e16842, Feb. 03 2011 , Refereed
    Summary:BACKGROUND: We had previously identified the mutant allele of apm1(+) that encodes a homolog of the mammalian µ1A subunit of the clathrin-associated adaptor protein-1 (AP-1) complex, and we demonstrated the role of Apm1 in Golgi/endosome trafficking, secretion, and vacuole fusion in fission yeast. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we isolated rho3(+), which encodes a Rho-family small GTPase, an important regulator of exocystosis, as a multicopy-suppressor of the temperature-sensitive growth of the apm1-1 mutant cells. Overexpression of Rho3 suppressed the Cl(-) sensitivity and immunosuppressant sensitivity of the apm1-1 mutant cells. Overexpression of Rho3 also suppressed the fragmentation of vacuoles, and the accumulation of v-SNARE Syb1 in Golgi/endosomes and partially suppressed the defective secretion associated with apm1-deletion cells. Notably, electron microscopic observation of the rho3-deletion cells revealed the accumulation of abnormal Golgi-like structures, vacuole fragmentation, and accumulation of secretory vesicles; these phenotypes were very similar to those of the apm1-deletion cells. Furthermore, the rho3-deletion cells and apm1-deletion cells showed very similar phenotypic characteristics, including the sensitivity to the immunosuppressant FK506, the cell wall-damaging agent micafungin, Cl(-), and valproic acid. Green fluorescent protein (GFP)-Rho3 was localized at Golgi/endosomes as well as the plasma membrane and division site. Finally, Rho3 was shown to form a complex with Apm1 as well as with other subunits of the clathrin-associated AP-1 complex in a GTP- and effector domain-dependent manner. CONCLUSIONS/SIGNIFICANCE: Taken together, our findings reveal a novel role of Rho3 in the regulation of Golgi/endosome trafficking and suggest that clathrin-associated adaptor protein-1 and Rho3 co-ordinate in intracellular transport in fission yeast. To the best of our knowledge, this study provides the first evidence of a direct link between the small GTPase Rho and the clathrin-associated adaptor protein-1 in membrane trafficking.
  • Role of RNA-Binding Proteins in MAPK Signal Transduction Pathway., Reiko Sugiura, Ryosuke Satoh, Shunji Ishiwata, Nanae Umeda, Ayako Kita, Journal of signal transduction, Journal of signal transduction, 2011, 109746 - 109746, 2011 , Refereed
    Summary:Mitogen-activated protein kinases (MAPKs), which are found in all eukaryotes, are signal transducing enzymes playing a central role in diverse biological processes, such as cell proliferation, sexual differentiation, and apoptosis. The MAPK signaling pathway plays a key role in the regulation of gene expression through the phosphorylation of transcription factors. Recent studies have identified several RNA-binding proteins (RBPs) as regulators of MAPK signaling because these RBPs bind to the mRNAs encoding the components of the MAPK pathway and regulate the stability of their transcripts. Moreover, RBPs also serve as targets of MAPKs because MAPK phosphorylate and regulate the ability of RBPs to bind and stabilize target mRNAs, thus controlling various cellular functions. In this review, we present evidence for the significance of the MAPK signaling in the regulation of RBPs and their target mRNAs, which provides additional information about the regulatory mechanism underlying gene expression. We further present evidence for the clinical importance of the posttranscriptional regulation of mRNA stability and its implications for drug discovery.
  • Evaluation of brilliance and visibility of fluorescence and chemiluminescence solution for training of preparing injections., Shunji Ishiwata, Atsushi Taga, Hiroyuki Sano, Masataka Kobayashi, Jun Nomiyama, Shiro Harada, Ayako Kita, Mitsutaka Takada, Reiko Sugiura, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 131(9), 1361 - 7, 2011 , Refereed
    Summary:Personnel who prepare and administer chemotherapeutic agents have been reported to develop untoward effects. The use of appropriate techniques for preparing these agents is encouraged, and educational training systems that involve the use of a fluorescent or chemiluminescence reagent as placebos have been established to minimize potential exposure to these agents. However, the optimum conditions for the use and visibility of these placebos remain obscure. In this study, our results indicated that the fluorescence intensity of fluorescent reagent decreased when it was used at a concentration greater than 0.01%. Because drops created due to splashes and leaks are extremely small and easily evaporate, it is possible that the fluorescence resulting from such drops readily disappears despite using an anti-evaporation reagent. We also developed a method to evaluate the visibility of the small drop; using this method, we determined the distance at which the drop present on the pin could be seen by the observer. The distance at which the drop was clearly recognized as a pinpoint by using the fluorescence method was almost comparable to that for the chemiluminescence method. In the chemiluminescence method, the drop on the pin was faintly visible as a slightly bright area because of low background when observed at a certain distance that was much greater than that at which the drop was clearly visible; however, such an area was not observed in the fluorescence method. The results of our study will help in the selection of a training method depending on the situation.
  • In vitro assay of the interaction between Rnc1 protein and Pmp1 mRNA by affinity capillary electrophoresis with a carboxylated capillary., Atsushi Taga, Ryosuke Satoh, Shunji Ishiwata, Shuji Kodama, Atsushi Sato, Kentaro Suzuki, Reiko Sugiura, Journal of pharmaceutical and biomedical analysis, Journal of pharmaceutical and biomedical analysis, 53(5), 1332 - 7, Dec. 15 2010 , Refereed
    Summary:The interaction between Rnc1, an RNA interactive protein, and a Pmp1 mRNA was investigated by affinity capillary electrophoresis (ACE). Prior to the ACE experiments, the column performances of three capillaries (an untreated fused silica capillary, a polybrene-polyacrylic acid (PB-PAA) double layer coating capillary, and a carboxylated capillary with a covalent modification) were studied with model proteins including ribonuclease B (RNase B) and bovine serum albumin (BSA). Using an untreated fused silica and a PB-PAA double layer coating capillaries, both of the protein peaks were broad and tailing. However, using a carboxylated capillary, the protein peaks were sharp and symmetric, and migration times were repeatable (RSD<0.4%). Further, the proteins in human serum also gave sharp peaks and its repeatability was kept at a high level by pre-treatment of a capillary inner wall with 1M sodium chloride solution before each run. An Rnc1 protein was analyzed by ACE with background electrolytes containing various concentrations of Pmp1 sense mRNA using a carboxylated capillary. Increase in the concentration of the mRNA was found to delay the migration time of the protein. But the migration time of the protein was kept constant with increasing Pmp1 anti-sense mRNA instead of Pmp1 sense mRNA. A straight line (r=0.987) was obtained by plotting 1/(migration time shift) versus 1/(Pmp1 sense mRNA concentration) and the association constant of Rnc1 protein with Pmp1 sense mRNA could be estimated to be 4.15x10(6)M(-1). These results suggest that the association constants of proteins with mRNAs as ligands were easily determined by the proposed method.
  • The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast., Hirofumi Takada, Aiko Nishida, Mitsuhiro Domae, Ayako Kita, Yuki Yamano, Atsushi Uchida, Shunji Ishiwata, Yue Fang, Xin Zhou, Takashi Masuko, Mitsuhiro Kinoshita, Kazuaki Kakehi, Reiko Sugiura, Molecular biology of the cell, Molecular biology of the cell, 21(4), 674 - 85, Feb. 15 2010 , Refereed
    Summary:The highly conserved fission yeast Pmk1 MAPK pathway plays a key role in cell integrity by regulating Atf1, which belongs to the ATF/cAMP-responsive element-binding (CREB) protein family. We identified and characterized ecm33(+), which encodes a glycosyl-phosphatidylinositol (GPI)-anchored cell surface protein as a transcriptional target of Pmk1 and Atf1. We demonstrated that the gene expression of Ecm33 is regulated by two transcription factors Atf1 and a MADS-box-type transcription factor Mbx1. We identified a putative ATF/CREB-binding site and an RLM1-binding site in the ecm33(+) promoter region and monitored the transcriptional activity of Atf1 or Mbx1 in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of the CRE and six tandem repeats of the Rlm1-binding sequence, respectively. These reporter genes reflect the activation of the Pmk1 pathway by various stimuli, thereby enabling the real-time monitoring of the Pmk1 cell integrity pathway. Notably, the Deltaecm33 cells displayed hyperactivation of the Pmk1 signaling together with hypersensitivity to Ca(2+) and an abnormal morphology, which were almost abolished by simultaneous deletion of the components of the Rho2/Pck2/Pmk1 pathway. Our results suggest that Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling.
  • Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast., Ryosuke Satoh, Takahiro Morita, Hirofumi Takada, Ayako Kita, Shunji Ishiwata, Akira Doi, Kanako Hagihara, Atsushi Taga, Yasuhiro Matsumura, Hideki Tohda, Reiko Sugiura, Molecular biology of the cell, Molecular biology of the cell, 20(9), 2473 - 85, May 2009 , Refereed
    Summary:Myosin II is an essential component of the actomyosin contractile ring and plays a crucial role in cytokinesis by generating the forces necessary for contraction of the actomyosin ring. Cdc4 is an essential myosin II light chain in fission yeast and is required for cytokinesis. In various eukaryotes, the phosphorylation of myosin is well documented as a primary means of activating myosin II, but little is known about the regulatory mechanisms of Cdc4. Here, we isolated Nrd1, an RNA-binding protein with RNA-recognition motifs, as a multicopy suppressor of cdc4 mutants. Notably, we demonstrated that Nrd1 binds and stabilizes Cdc4 mRNA, thereby suppressing the cytokinesis defects of the cdc4 mutants. Importantly, Pmk1 mitogen-activated protein kinase (MAPK) directly phosphorylates Nrd1, thereby negatively regulating the binding activity of Nrd1 to Cdc4 mRNA. Consistently, the inactivation of Pmk1 MAPK signaling, as well as Nrd1 overexpression, stabilized the Cdc4 mRNA level, thereby suppressing the cytokinesis defects associated with the cdc4 mutants. In addition, we demonstrated the cell cycle-dependent regulation of Pmk1/Nrd1 signaling. Together, our results indicate that Nrd1 plays a role in the regulation of Cdc4 mRNA stability; moreover, our study is the first to demonstrate the posttranscriptional regulation of myosin expression by MAPK signaling.
  • Immunoprecipitation and MALDI-MS identification of lithocholic acid-tagged proteins in liver of bile duct-ligated rats., Shigeo Ikegawa, Tetsushi Yamamoto, Hiromi Ito, Shunji Ishiwata, Toshihiro Sakai, Kuniko Mitamura, Masako Maeda, Journal of lipid research, Journal of lipid research, 49(11), 2463 - 73, Nov. 2008 , Refereed
    Summary:Formation of covalently bound protein adducts with lithocholic acid (LCA) might explain LCA's known carcinogenic properties and hepatotoxicity. We performed studies aimed at isolating and identifying hepatic proteins tagged with LCA, presumably via the epsilon-amino group of lysine residues. Antibodies recognizing the 3alpha-hydroxy-5beta-steroid moiety of LCA were generated by immunizing rabbits with immunogens in which the carboxyl group of LCA was coupled to BSA via a 6-aminohexanoic acid and/or succinic acid spacer. The resulting antibodies reacted with N-alpha-(t-butoxycarbonyl)-l-lysine-epsilon-LCA, the amidated and nonamidated forms of LCA, as well as synthetically prepared LCA adducts with ovalbumin and lysozyme. Proteins tagged with LCA in the liver of bile duct-ligated rats were isolated by immunoprecipitation using these antibodies. Proteins were isolated by two-dimensional electrophoresis, and their structure was identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry and computer-assisted programs. Proteins labeled with LCA were Rab-3, Rab-12, Rab-16, and M-Ras. Rab proteins are Ras-like small GTP binding proteins that regulate vesicle trafficking pathways. The covalent binding of the Rab proteins with LCA may influence vesicular transport or binding of vesicles to their cognate membrane and may contribute to LCA-induced liver toxicity.
  • Production and characterization of a monoclonal antibody to capture proteins tagged with lithocholic acid., Shigeo Ikegawa, Tetsushi Yamamoto, Takahiro Miyashita, Rika Okihara, Shunji Ishiwata, Toshihiro Sakai, Rung-Hwa Chong, Masako Maeda, Alan F Hofmann, Kuniko Mitamura, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 24(11), 1475 - 80, 2008 , Refereed
    Summary:Reactive metabolic-modified proteins have been proposed to play an important role in the mechanism(s) of the hepatotoxicity and colon cancer of lithocholic acid (LCA). To identify cellular proteins chemically modified with LCA, we have generated a monoclonal antibody that recognizes the 3alpha-hydroxy-5beta-steroid moiety of LCA. The spleen cells from a BALB/c mouse, which was immunized with an immunogen in which the side chain of LCA was coupled to bovine serum albumin (BSA) via a succinic acid spacer, was fused with SP2/0 myeloma cells to generate antibody-secreting hybridoma clones. The resulting monoclonal antibody (gamma2b, kappa) was specific to LCA-N(alpha)-BOC-lysine as well as the amidated and nonamidated forms of LCA. The immunoblot enabled the detection of LCA residues anchored on BSA and lysozyme. The antibody will be useful for monitoring the generation, localization, and capture of proteins tagged with LCA, which may be the cause of LCA-induced toxicity.
  • Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast., Hirofumi Takada, Masayuki Nishimura, Yuta Asayama, Yoshiaki Mannse, Shunji Ishiwata, Ayako Kita, Akira Doi, Aiko Nishida, Naoyuki Kai, Sayako Moriuchi, Hideki Tohda, Yuko Giga-Hama, Takayoshi Kuno, Reiko Sugiura, Molecular biology of the cell, Molecular biology of the cell, 18(12), 4794 - 802, Dec. 2007 , Refereed
    Summary:In fission yeast, knockout of the calcineurin gene resulted in hypersensitivity to Cl(-), and the overexpression of pmp1(+) encoding a dual-specificity phosphatase for Pmk1 mitogen-activated protein kinase (MAPK) or the knockout of the components of the Pmk1 pathway complemented the Cl(-) hypersensitivity of calcineurin deletion. Here, we showed that the overexpression of ptc1(+) and ptc3(+), both encoding type 2C protein phosphatase (PP2C), previously known to inactivate the Wis1-Spc1-Atf1 stress-activated MAPK signaling pathway, suppressed the Cl(-) hypersensitivity of calcineurin deletion. We also demonstrated that the mRNA levels of these two PP2Cs and pyp2(+), another negative regulator of Spc1, are dependent on Pmk1. Notably, the deletion of Atf1, but not that of Spc1, displayed hypersensitivity to the cell wall-damaging agents and also suppressed the Cl(-) hypersensitivity of calcineurin deletion, both of which are characteristic phenotypes shared by the mutation of the components of the Pmk1 MAPK pathway. Moreover, micafungin treatment induced Pmk1 hyperactivation that resulted in Atf1 hyperphosphorylation. Together, our results suggest that PP2C is involved in a negative feedback loop of the Pmk1 signaling, and results also demonstrate that Atf1 is a key component of the cell integrity signaling downstream of Pmk1 MAPK.
  • Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases., Seiichi Shinji, Zenya Naito, Shunji Ishiwata, Toshiyuki Ishiwata, Noritake Tanaka, Kiyonori Furukawa, Hideyuki Suzuki, Tomoko Seya, Akihisa Matsuda, Miwako Katsuta, Takashi Tajiri, Oncology reports, Oncology reports, 15(3), 539 - 43, Mar. 2006 , Refereed
    Summary:Ubiquitin-specific protease 14, also known as the 60 kDa subunit of tRNA-guanine transglycosylase (USP14/TGT60 kD), belongs to the ubiquitin-specific processing protease (UBP) family. USP14/TGT60 kD expression in leukemic and colorectal cancer cell lines, and the suppression of such an expression after the induction of cell differentiation have been reported. In the present study, we attempted to clarify whether USP14/TGT60 kD overexpression affects the clinicopathological features of colorectal cancer. Immunohistochemically, USP14/TGT60 kD was absent or weakly localized in the cytoplasm of normal colorectal epithelial cells. In 18 of 99 (18.2%) colorectal cancer patients, USP14/TGT60 kD was strongly detected in the cytoplasm of cancer cells. USP14/TGT60 kD expression correlated with pathological stage (P=0.03), and lymph node (P=0.03) and liver (P=0.03) metastases. Furthermore, the percentage of patients strongly positive for USP14/TGT60 kD expression increased with pathological stage. The overall survival rate was worse in patients with a high USP14/TGT60 kD expression level than in those with a low USP14/TGT60 kD expression level. Our results suggest that USP14/TGT60 kD also controls the fate of proteins that regulate tumor invasion and metastasis.
  • Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer., Shunji Ishiwata, Yasuko Ozawa, Jun Katayama, Shuko Kaneko, Hiroki Shindo, Yoshihisa Tomioka, Toshiyuki Ishiwata, Goro Asano, Shigeo Ikegawa, Michinao Mizugaki, Cancer letters, Cancer letters, 212(1), 113 - 9, Aug. 20 2004 , Refereed
    Summary:tRNA-guanine transglycosylase (TGT) is an enzyme which synthesizes a modified nucleoside, queuosine, by exchanging the base moiety of guanosine for queuine in tRNA. We have reported that the expression level of the 60-kDa subunit of TGT (TGT60kD) is elevated in leukemic cells, however, there is no other report on the expression of TGT60kD in cancer cells. The expression levels of the TGT60kD protein are elevated in four of the five colon cancer cell lines and 83% of colon cancer tissues compared with normal tissues. The expression levels of the TGT60kD protein decreased in two colon cancer cell lines, after cell differentiation was induced. A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis.
  • Enantioselective immunorecognition of protein modification with optically active ibuprofen using polyclonal antibody., Hiromi Ito, Shunji Ishiwata, Takeshi Kosaka, Rika Nakashima, Harunori Takeshita, Sakiko Negoro, Masako Maeda, Shigeo Ikegawa, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 806(1), 11 - 7, Jun. 25 2004 , Refereed
    Summary:Formation of covalently bound protein adducts with 2-arylpropionic acids (2-APAs) has been proposed as a possible explanation for hypersensitivity and toxic responses to chiral carboxylic acid drugs. To identify the cellular proteins chemically modified with optically active (S)-ibuprofen, we generate polyclonal antibodies by immunizing rabbits with immunogen coupled to bovine serum albumin (BSA) via the spacer of 4-aminobutyric acid. The resulting antibodies largely cross-reacted with N-alpha-(t-butoxycarbonyl)--(S)-ibuprofenyl lysine as well as with the conjuguated (S)-ibuprofen with glycine and taurine and unconjugated (S)-ibuprofen, enabling enantioselective detection of (S)-ibuprofen residues anchored on ovalbumin molecules, introduced by the reaction of the ibuprofen p-nitrophenyl ester. Furthermore, immunoblotting with an antibody allows the enantioselective detection of (S)-ibuprofen-introduced glutathione-S-transferase (GST). These results indicate that the developed method will be useful for monitoring the generation and localization of protein covalently bound with (S)-ibuprofen, which may be the cause of ibuprofen-induced toxicity.
  • Effect of morpholino antisense oligonucleotide against lumican mRNA in human embryonic kidney (HEK) 293 cells., Toshiyuki Ishiwata, Takenori Fujii, Shunji Ishiwata, Shigeo Ikegawa, Zenya Naito, Pathology international, Pathology international, 54(2), 77 - 81, Feb. 2004 , Refereed
    Summary:Lumican is a member of the small-leucine-rich proteoglycan (SLRP) family and is overexpressed during wound healing of the cornea, in ischemic and reperfused heart, and in several cancer tissues. Lumican is considered to regulate the collagen fibril diameter and interfibrillar spacing. However, the effect of lumican on cell growth has not been adequately examined. In the present study, we attempted to clarify whether lumican contributes to human embryonic kidney (HEK) 293 cell growth, using the morpholino antisense oligonucleotide (m-anti oligo) against lumican mRNA. M-anti oligo is a novel oligonucleotide and exhibits a higher antisense activity, higher water solubility, and greater resistance to nucleases in target cells than phosphorothioate types of oligonucleotide. After delivery of m-anti oligo against lumican mRNA, the fluorescein 5-isothiocyanate (FITC) conjugated oligonucleotides were observed in the cytoplasm and nucleus of HEK 293 cells at 24 h by confocal laser microscopy. M-anti oligo for lumican mRNA strongly inhibited the synthesis of lumican protein in the HEK 293 cells, and the HEK cell growth rate was higher than those in the control groups. These findings may indicate that lumican protein has an inhibitory effect on HEK 293 cell growth in vitro.

Books etc

  • Sourcebook of Models for Biomedical Research, Humana Press Inc., Molecular Genetic Approach to Identify Inhibitors of Signal Transduction Pathways., Joint author,   2008

Conference Activities & Talks

  • Spatial regulatory mechanism of the PI4,5P2 signaling pathway by membrane trafficking system,   2011 06
  • MAP kinase signaling regulates stress granule formation via the RNA-binding protein Nrd1,   2011 06
  • The MADS-box transcription factor Mbx1 controls Ca2+ homeostasis via the regulation of dom1+,   2011 06
  • MAP kinase signaling-dependent regulation of stress granule formation mediated by the RNA-binding protein Nrd1 in fission yeast,   2011 06
  • MADS-Box Transcription Factor Mbx1 Is Involved in the Regulation of Calcineurin Signaling through Ca2+-Homeostasis Modulation, 1st International Symposium on Carcinogenic Spiral & 9th International Conference on Protein Phospha,   2011 02 , 1st International Symposium on Carcinogenic Spiral & 9th International Conference on Protein Phospha
  • The Actin Binding Protein Cis3/PSTPIP Is Involved in the Regulation of the Pmk1 MAPK Signaling Pathway and Cytokinesis, 1st International Symposium on Carcinogenic Spiral & 9th International Conference on Protein Phospha,   2011 02 , 1st International Symposium on Carcinogenic Spiral & 9th International Conference on Protein Phospha
  • MAP Kinase Signaling Dependent Regulation of Cell Fate Mediated by the RNA-binding protein Nrd1 in Fission yeast, The 19th CDB Meeting RNA Sciences in Cell and Developmental Biology,   2010 05 , The 19th CDB Meeting RNA Sciences in Cell and Developmental Biology
  • Functional Relationship between MAP kinase Signaling and Pumilio in Fission Yeast, The 19th CDB Meeting RNA Sciences in Cell and Developmental Biology,   2010 05 , The 19th CDB Meeting RNA Sciences in Cell and Developmental Biology
  • MAPK SIGNALING-DEPENDENT REGULATION OF CELL FATE MEDIATED BY THE RNA-BINDING PROTEIN Nrd1, The 5th International Fission Yeast Meeting (Pombe 2009),   2009 10 , The 5th International Fission Yeast Meeting (Pombe 2009)
  • A NOVEL CELL SURFACE PROTEIN TPA1 ACTS AS A NEGATIVE REGULATOR OF THE PMK1 MAPK SIGNALING, The 5th International Fission Yeast Meeting (Pombe 2009),   2009 10 , The 5th International Fission Yeast Meeting (Pombe 2009)
  • The Implementation of the Model Core Curriculum toward Hospital Pharmacy Practice Experience,   2009 03
  • The regulatory mechanism of cell-fate switching by MAPK signaling and the RNA-binding protein Nrd1, 8th International Conference on Protein Phosphatases,   2008 11 , 8th International Conference on Protein Phosphatases
  • The Actin Binding Protein Cis3/PSTPIP Plays a Role in Activation of MAPK Signaling Pathways, 8th International Conference on Protein Phosphatases,   2008 11 , 8th International Conference on Protein Phosphatases
  • Cross-talk between Calcineurin Signaling and the MADS-box Transcription Factor Mbx1 in the Ca2+ Homeostasis in Fission Yeast, 8th International Conference on Protein Phosphatases,   2008 11 , 8th International Conference on Protein Phosphatases
  • The Atf1 Transcription Factor Is a Target of the Mitogen-activated Protein Kinase Pmk1 and Regulates the Expression of the tpa1+ Gene, which Encodes a Novel Cell Surface Protein in Fission Yeast, 8th International Conference on Protein Phosphatases,   2008 11 , 8th International Conference on Protein Phosphatases
  • Cbp1, an Endogenous Inhibitor of Calcineurin Signaling, Acts as a Negative Regulator of Atf1, a Downstream Transcription Factor of the Stress-activated MAP Kinase Pathway, 8th International Conference on Protein Phosphatases,   2008 11 , 8th International Conference on Protein Phosphatases
  • Observation of Interaction between RNA and GFP-Protein by Affinity Capillary Electrophoresis, 29 th International Symposium on the ?Separation of Proteins, Peptides and Polynucleotides,   2008 09 , 29 th International Symposium on the ?Separation of Proteins, Peptides and Polynucleotides
  • Study of RNA-protein interaction in MAP kinase signaling by affinity capillary electrophoresis with a carboxylated capillary, 28 th International Symposium on the ?Separation of Proteins, Peptides and Polynucleotides,   2008 09 , 28 th International Symposium on the ?Separation of Proteins, Peptides and Polynucleotides
  • The Atf1 Transcription Factor acts as a critical signaling node that links cell integrity signal and stress responses mediated by two distinct MAPK pathways., The 21st Naito Conference-Nuclear Dynamics and RNA [I],   2008 06 , The 21st Naito Conference-Nuclear Dynamics and RNA [I]
  • The Implementation of the Model Core Curriculum toward Pharmacy Practice Experience for 2-4 weeks,   2008 03
  • Development of a novel type capillary for protein analysis by capillary electrophoresis, EUROanalysis XIV,   2007 09 , EUROanalysis XIV
  • Functional interaction between Pumilio family and cell integrity signaling in fission yeast.,   2007 07
  • Apm1, theμ1 subunit of the Clathrin-Associated Adaptor Protein-1 Complex, Shares an essential function with Calcineurin in fission yeast.,   2007 07
  • The identical and phenotypic characterization of an endogenous calcineurin inhibitor Cbp1 in fission yeast.,   2007 07
  • A Survey on the Students’ Attitudes toward Pharmacy Practice Experience in Kinki University,   2007 03

Misc

  • クラウド上に構築した災害時医薬品管理システムとその評価, 北小路 学, 石渡 俊二, 村瀬 惇, 井上 知美, 大島 徹, 松野 純男, 小竹 武, 日本薬学会年会要旨集, 138年会, 4, 181, 181,   2018 03
  • 薬局薬剤師のコミュニケーション関連業務と性格分析の相関性について, 村田 彩純, 梶本 青午, 井上 知美, 石渡 俊二, 小竹 武, 土居 弘明, 堀越 勝博, 重森 裕之, 日本薬学会年会要旨集, 138年会, 4, 226, 226,   2018 03
  • 薬剤師を対象とした二次救命処置講習会の評価, 井上 知美, 小島 理恵, 岡田 ひとみ, 有元 秀樹, 窪田 愛恵, 平出 敦, 西田 升三, 石渡 俊二, 小竹 武, 日本薬学会年会要旨集, 138年会, 4, 226, 226,   2018 03
  • 各種温度下におけるシクロホスファミドの最大ガス化量, 遠藤晋吾, 遠藤晋吾, 石渡俊二, 山本理恵, 北野里佳, 藤本美弥, 山本哲志, 多賀淳, 升永早紀, 益子高, 福田由之, 安井友佳子, 石坂敏彦, 井上知美, 小竹武, 日本薬学会年会要旨集(CD-ROM), 138th, ROMBUNNO.28PA‐am347S,   2018 , http://jglobal.jst.go.jp/public/201802281009171344
  • Investigation of simulated patient’s attitudes toward physical assessment task, 大鳥徹, 井上知美, 細見光一, 石渡俊二, 藤本麻依, 北小路学, 小竹武, 薬学教育(Web), 2, ROMBUNNO.2017‐017(J‐STAGE),   2018 , 10.24489/jjphe.2017-017, https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201902240389987163
  • Investigation of simulated patient's attitudes toward physical assessment task, Otori Toru, Inoue Tomomi, Hosomi Koichi, Ishiwata Shunji, Fujimoto Mai, Kitakoji Manabu, Kotake Takeshi, Japanese Journal of Pharmaceutical Education, 2, 0,   2018 , http://ci.nii.ac.jp/naid/130006387039
    Summary:<p>Since the passing of the (guidelines aimed to augment the role of the pharmacists in 2010) pharmacists are required to conduct patient vital checks. As a result, the Pharmaceutical Education Model Core Curriculum require physical assessment (PA) competencies of students. In order to carry out physical assessment task, volunteer subjects need to be recruited and trained to perform the role of simulated patients (SPs). However, to date, there are few investigations into SP attitudes toward PA. The purpose of this study is to conduct a questionnaire to investigate SP attitudes towards PA, and their understanding of the current state of the home medical care system. Responses from the questionnaire show volunteer SPs had little knowledge about PA and the home medical care system. During vital check examinations, SPs were open to having their blood measured but were averse to auscultation. This bias was stronger in woman than men. In terms of future considerations, the questionnaire results suggest volunteer SPs need to be instructed on the benefits of having comprehensive vital check assessments as well as understand the meaning of the role they are asked to play in order to better authenticate the patient/pharmacist relationship. This is particularly important as the performance of vital checks have expanded to become a part of home medical care practices.</p>
  • 被災地で医薬品を効率的に活用するためのクラウドネットワーク, 石渡 俊二, 北小路 学, 井上 知美, 大鳥 徹, 小竹 武, 日本職業・災害医学会会誌, 65, 臨増, 別254, 別254,   2017 11
  • 心疾患患者における薬剤師の今後の役割と課題~フィジカルアセスメント~, 井上知美, 大鳥徹, 細見光一, 石渡俊二, 西田升三, 小竹武, 日本心臓病学会学術集会(Web), 65th, ROMBUNNO.CS4‐2 (WEB ONLY),   2017 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702224726139292
  • Survey on Customer Satisfaction for Evaluation and Improvement of Physical Assessment Practical Training Seminar for Pharmacists, 大鳥徹, 井上知美, 細見光一, 中川博之, 高島敬子, 近藤尚美, 高田亜美, 伊藤栄次, 中山隆志, 和田哲幸, 石渡俊二, 前川智弘, 船上仁範, 中村真也, 窪田愛恵, 平出敦, 松山賢治, 西田升三, 社会薬学, 35, 2, 94‐101, 101,   2016 12 10 , 10.14925/jjsp.35.2_94, http://jglobal.jst.go.jp/public/201702220578908674
    Summary:<p>In the areas of home medical care and self-medication, the role of the pharmacist is growing, partly as a result of Japan's aging society and the need to reduce medical costs. In response, the Kinki University Faculty of Pharmacy implemented a physical assessment practical training seminar in order to improve the physical assessment skills of practicing pharmacists. A series of questionnaires were conducted among pharmacists to investigate their perceptions of physical assessment practical training seminars. The results of the questionnaires were analyzed using Customer Satisfaction (CS) analysis and text mining. Based on a 5-point scale (1-low∼5-high), questionnaires revealed satisfaction for physical assessment practical training seminars was 4.6±0.6 (Ave.±S.D.). CS analysis revealed that the items "lectures" and "case seminars" had the highest level of satisfaction. However, items showing low levels of satisfaction were "auscultation of respiratory sounds" and "SBAR (Situation, Background, Assessment, Recommendation)." Results of text mining suggested a relationship between "physical assessment" and "difficult". Analysis of the questionnaires showed a high level satisfaction with physical assessment practical training seminars, notably physical assessment practice methods. However, CS analysis and text mining indicate the finer techniques of physical assessment were difficult to acquire.</p>
  • CS分析(Customer Satisfaction analysis)による薬剤師のためのフィジカルアセスメント講習会の評価と改善, 大鳥 徹, 井上 知美, 細見 光一, 中川 博之, 高島 敬子, 近藤 尚美, 高田 亜美, 伊藤 栄次, 中山 隆志, 和田 哲幸, 石渡 俊二, 前川 智弘, 船上 仁範, 中村 真也, 窪田 愛恵, 平出 敦, 松山 賢治, 西田 升三, 社会薬学, 35, 2, 94, 101,   2016 12 , 10.14925/jjsp.35.2_94
    Summary:フィジカルアセスメント講習会に参加した143名を対象にアンケートを実施した。CS分析(Customer Satisfaction analysis)とテキストマイニングにより検討した。男性が39%、女性が61%と女性が多かった。勤務先は保険調剤薬局が38%、病院が62%となっており、病院勤務者が多かった。改善度は、数値が大きいほど改善の優先順位は高くなるが、改善度の値が大きい順に五つ項目を上げると、「心電図の読み方」「呼吸音の聴診」「SBAR」「時間は適当でしたか」「心音の聴診」であった。CSグラフにおいて、優先的に改善する必要がある項目がプロットされる「要改善項目」(第4象限)として抽出されてきたのは、「呼吸音の聴診」、「SBAR」であった。「心音・腸音の聴診」、「心電図の読み方」、「血圧測定」は評価が低く、総合評価への影響度も低い項目である「改善検討項目」として第3象限にプロットされた。「講義内容」、「症例に興味が持てたか」、「フィジカルアセスメントモデルの機能」、「時間は適当でしたか」は評点が高く、総合評価への影響度も高い項目である「重要維持項目」として第1象限にプロットされた。
  • 介護施設入所者における薬物療法の関連性指標の抽出, 坂本 紀夫, 新谷 怜莉, 細川 かおる, 伴 信人, 新井 邦彦, 萩原 成彦, 井上 知美, 石渡 俊二, 小竹 武, 池田 清美, 日本薬剤師会学術大会講演要旨集, 49回, P, 238,   2016 10
  • トラムセット配合錠上市前後の疼痛管理薬物療法における処方比較による薬学的管理情報の抽出, 北野 里佳, 細川 かおる, 新谷 怜莉, 伴 信人, 坂本 紀夫, 新井 邦彦, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 49回, P, 250,   2016 10
  • 睡眠導入薬処方の薬物治療における年齢・性別での比較検討, 藤本 美弥, 中島 礼子, 新谷 怜莉, 細川 かおる, 伴 信人, 坂本 紀夫, 新井 邦彦, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 49回, P, 251,   2016 10
  • 抗凝血薬(ワルファリン、ダビガトラン)の使用動向と併用薬の解析, 村田 彩純, 伴 信人, 新谷 怜莉, 細川 かおる, 中島 礼子, 坂本 紀夫, 新井 邦彦, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 49回, P, 254,   2016 10
  • 当薬局におけるビスホスホネート製剤の使用動向および併用薬解析による薬学的管理因子の抽出, 中島 礼子, 坂本 紀夫, 伴 信人, 細川 かおる, 森川 友紀子, 新谷 怜莉, 新井 邦彦, 西浦 広将, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 48回, 296, 296,   2015 11
  • 当薬局における非定型抗精神病薬の併用薬解析における比較検証, 伴 信人, 坂本 紀夫, 中島 礼子, 細田 康子, 南埜 裕貴子, 細川 かおる, 新井 邦彦, 植野 春奈, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 48回, 300, 300,   2015 11
  • 認知症研修会アンケートのクラスター分析による階層的解析について, 樋口 紗織, 坂本 紀夫, 新谷 怜莉, 中島 礼子, 清水 俊希, 井上 知美, 石渡 俊二, 小竹 武, 島田 由佳, 神原 明夫, 田齊 秀章, 日本薬剤師会学術大会講演要旨集, 48回, 315, 315,   2015 11
  • 介護施設入所者における血圧・臨床検査値と薬物療法の関連性因子について, 坂本 紀夫, 荒木 有希子, 上田 愛子, 八木 亮平, 上田 恭子, 八重樫 柊穂, 冨田 ゆり絵, 井上 知美, 石渡 俊二, 小竹 武, 池田 清美, 日本薬剤師会学術大会講演要旨集, 48回, 342, 342,   2015 11
  • 環境負荷の小さな化学物質を用いた土壌中放射性セシウムの溶出の検討, 石渡 俊二, 多賀 淳, 緒方 文彦, 北小路 学, 山西 弘城, 稲垣 昌代, 伊藤 哲夫, 古川 道郎, 日本保健物理学会研究発表会講演要旨集, 48回, 114, 114,   2015 07
  • 災害時医薬品供給のためのストック確保に関する検討, 北小路 学, 加藤 寛, 石渡 俊二, 櫻田 ゆかり, 鎌塚 正人, 栗栖 勝, 安藤 和佳子, 井上 知美, 松山 賢治, 小竹 武, 日本薬学会年会要旨集, 135年会, 4, 156, 156,   2015 03
  • 薬学的管理におけるシタグリプチンとビルダグリプチンの併用薬の比較解析, 下浦 達明, 渡邉 幸子, 礒脇 雄一, 遠藤 晋吾, 井上 知美, 石渡 俊二, 小竹 武, 日本薬学会年会要旨集, 135年会, 4, 189, 189,   2015 03
  • クラウド上に構築した災害時支援医薬品供給管理システム, 北小路 学, 加藤 寛, 石渡 俊二, 安藤 和佳子, 安達 秀樹, 鵜山 泰詳, 櫻田 ゆかり, 井上 知美, 松山 賢治, 小竹 武, 日本集団災害医学会誌, 19, 3, 494, 494,   2014 12
  • 当薬局における抗精神病薬の時系列使用変遷の解析, 伴 信人, 前田 栄子, 中島 礼子, 細川 かおる, 坂本 紀夫, 新井 邦彦, 岡本 卓也, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 47回, 305, 305,   2014 10
  • 当薬局におけるピロリ除菌薬の使用状況と除菌効果の解析について, 辰巳 和代, 細川 かおる, 中島 礼子, 伴 信人, 坂本 紀夫, 新井 邦彦, 成田 雄亮, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 47回, 306, 306,   2014 10
  • 当薬局における抗アレルギー薬の使用変遷と季節性ニーズの解析, 中島 礼子, 森川 友紀子, 八木 亮平, 細川 かおる, 伴 信人, 坂本 紀夫, 新井 邦彦, 波夛 孝次朗, 井上 知美, 石渡 俊二, 小竹 武, 日本薬剤師会学術大会講演要旨集, 47回, 306, 306,   2014 10
  • 保険薬局医療従事者の認知症研修会の有用性について, 坂本 紀夫, 新井 邦彦, 清水 俊希, 井上 知美, 石渡 俊二, 小竹 武, 島田 由佳, 神原 明夫, 長谷川 肇, 田齋 秀章, 増田 修也, 日本薬剤師会学術大会講演要旨集, 47回, 367, 367,   2014 10
  • 福島第1原子力発電所事故に起因する土壌中放射性セシウムの分布,溶出,吸着に関する研究, YAMANISHI HIROKUNI, INAGAKI MASAYO, WAKABAYASHI GEN'ICHIRO, HOHARA SHIN'YA, ITO TETSUO, ISHIWATA SHUNJI, TAGA ATSUSHI, OGATA FUMIHIKO, FURUKAWA MICHIO, 日本保健物理学会研究発表会講演要旨集, 46th, 136,   2013 06 24 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302290314729695
  • セシウムを99%以上取り除く「ゼオCa漆喰」, TAGA ATSUSHI, ISHIWATA SHUNJI, OGATA FUMIHIKO, SATO YOICHI, MORIMURA TSUYOSHI, 日本薬学会年会要旨集(CD-ROM), 133rd, ROMBUNNO.29PME-095,   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302242463952025
  • 近畿大学薬学部における模擬患者養成の取り組み, 大島徹, 木村健, 安原智久, 北小路学, 船上仁範, 石渡俊二, 谷野公俊, 村上悦子, 八軒浩子, 高田充隆, 日本薬学会年会要旨集, 130th, 4, 334,   2010 03 05 , http://jglobal.jst.go.jp/public/201002255396122538
  • 分子遺伝学的手法を用いた新規MAPキナーゼ阻害薬の探索, 萬瀬貴昭, 高田宏文, 朝山雄太, 森田貴大, 安原智久, 村岡修, 喜多綾子, 石渡俊二, 杉浦麗子, 日本薬学会年会要旨集, 128th, 2, 49,   2008 03 05 , http://jglobal.jst.go.jp/public/200902273384877387
  • 分子遺伝学的手法を用いた新規MAPキナーゼスクリーニング法の確立・応用, 萬瀬貴昭, 高田宏文, 朝山雄太, 村岡修, 喜多綾子, 石渡俊二, 杉浦麗子, 日本薬学会年会要旨集, 127th, 2, 95, 95,   2007 03 05 , http://jglobal.jst.go.jp/public/200902293531159593
  • 保険薬局および病院実務実習に対する学生の意識調査, 北小路学, 石渡俊二, 大床真美子, 船上仁範, 八軒浩子, 多賀淳, 八木秀樹, 和田哲幸, 田邊元三, 市田成志, 西田升三, 社会薬学, 25, 2, 34,   2007 02 28 , http://jglobal.jst.go.jp/public/200902262219415130
  • ラット肝細胞内リトコール酸修飾蛋白質の捕捉と構造解析, 山本哲志, 伊藤裕美, 石渡俊二, 前田昌子, 後藤順一, 池川繁男, 日本薬学会年会要旨集, 124th, 3, 32,   2004 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902235698699956
  • 肝疾患の病態プロテオミクスに用いる分子標的機能性素子の創製, 池川繁男, 山本哲志, 伊藤裕美, 石渡俊二, 真野成康, 後藤順一, 前田昌子, バイオメディカル分析科学シンポジウム講演要旨集, 16th, 86, 87,   2003 08 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902278563717939
  • リトコール酸‐蛋白付加体の検出に用いるモノクローナル抗体の調製, 伊藤裕美, 大西恵, 山本哲志, 石渡俊二, 前田昌子, 真野成康, 後藤順一, 池川繁男, 日本薬学会年会要旨集, 123rd, 3, 55,   2003 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902260685364526
  • Enhanced expression of lumican inhibited the attachment and growth of human embryonic kidney 293 cells., Toshiyuki Ishiwata, Tetsushi Yamamoto, Kiyoko Kawahara, Yoko Kawamoto, Yoko Matsuda, Shunji Ishiwata, Zenya Naito, Experimental and molecular pathology, 88, 3, 363, 70,   2010 06 , Refereed, 10.1016/j.yexmp.2010.01.010
    Summary:Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression was reported in various kinds of tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in pancreatic cancer correlated with an advanced stage and retroperitoneal and duodenal invasion. In this study, we clarified whether the enhanced expression of lumican contributes to cellular attachment, growth, colony formation, migration and invasion. HEK 293 cell, stably transfected with lumican cDNA synthesized and secreted a 50 kDa lumican protein at high levels in culture medium. The cells showed a polygonal appearance with long projections and the degree of adhesion of the cells to fibronectin was lower than that of empty vector transfected control cells (mock cells). In contrast, the degree of adhesion of the cells to type I collagen was not different from that of mock cells. The expression levels of alpha5 integrin, the major integrin subunit for fibronectin, were lower in lumican-transfected HEK cells than in mock cells. Furthermore, lumican-transfected HEK cells showed reduced growth rates in vitro and did not form colonies in soft agar. Phosphorylation of AKT, extracellular signal-regulated kinase (ERK) 1/2 and mammalian target of rapamycin (mTOR) decreased in the lumican-transfected HEK cells. Cell migration and invasion were not altered in lumican-transfected HEK cells and mock cells. These findings indicate that the 50kDa lumican protein plays important roles in the inhibition of HEK cell attachment and growth, and it might inhibit the activation of integrin pathways.

Patents

  • The trainig kit and methods for anti-tumor cancer injection mixing, PCT/JP2010/66716
  • Real time assay method for MAP kinase by monitoring of mRNA and exploiting new anti-cancer drug, 特願2010-162093
  • Trainig kit and methods for anti-tumor cancer injection mixing, 特願2009-249404
  • Analysis of interaction between nucleic acid and protein by affinity capillary electrophoresis, 特願2009-207005