KINDAI UNIVERSITY


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KANZAKI Shinya

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FacultyDepartment of Agricultural Science / Graduate School of Agriculture / Experimental Farms
PositionAssociate Professor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/155-kanzaki-shinya.html
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Last Updated :2020/09/09

Research Activities

Research Areas

  • Environmental science/Agricultural science, Horticulture

Research Interests

  • Pomology

Published Papers

  • Analysis of genetic diversity of lychee (Litchi chinensis Sonn.) and wild forest relatives in the Sapindaceae from Vietnam using microsatellites, Hoa Tran, Shinya Kanzaki, Ludwig Triest, Inaki Hormaza, Na Jong Kuk, Ray Ming, Jean Bousquet, Damase Khasa, Patrick Van Damme, Genetic Resources and Crop Evolution, Genetic Resources and Crop Evolution, 66, 1653 - 1669, Dec. 01 2019
    Summary:© 2019, Springer Nature B.V. We report on 14 microsatellites enriched in CT repeats obtained from a genomic library of lychee (Litchi chinensis Sonn.) cultivar “Hong Huay”. The polymorphisms revealed by these microsatellites were evaluated in a collection of 45 local Vietnamese lychee varieties and 4 Xerospermum noronhianum (Blume) Blume (Sapindaceae) collected from the wild. Samples were collected from local villages and forests in northern Vietnam. Genetic diversity parameters were estimated for the local Vietnamese varieties analyzed. The unweighted pair-group method of clustering using averages divided the lychee cultivars into three main groups: Cluster 1 (Group A) consisting of semi-natural lychees (“extremely early” lychee); Cluster 2 (Group B) consisting of cultivated cultivars (“intermediate” lychee); and Cluster 3 (Group C) representing X. noronhianum accessions. Using STRUCTURE, three subpopulations were also delimited among litchi accessions, including accessions with extremely early- and intermediate/late-maturing traits showing membership coefficients above 0.99 for Cluster 1 and Cluster 2, respectively. Accessions with early- and intermediate-maturing traits were identified as admixture forms with varying levels of membership shared between the two clusters, indicating their hybrid origin during litchi domestication. This is the first report on transferability of SSR markers developed from lychee (L. chinensis) to X. noronhianum. Results demonstrate the usefulness of microsatellites for identification, genetic diversity analysis and germplasm conservation in lychee and related Sapindaceae forest species.
  • Practical marker-assisted selection using two SCAR markers for fruit astringency type in crosses of 'Taiten' x PCNA cultivars in persimmon breeding, Nobuhito Mitani, Atsushi Kono, Masahiko Yamada, Akihiko Sato, Shozo Kobayashi, Yusuke Ban, Toshihito Ueno, Mikio Shiraishi, Shinya Kanzaki, Tomoyuki Tsujimoto, Keizo Yonemori, SCIENTIA HORTICULTURAE, SCIENTIA HORTICULTURAE, 170, 219 - 223, May 2014 , Refereed
    Summary:Pollination-constant, non-astringent (PCNA) type persimmons (Diospyros kaki Thunb.) steadily lose fruit astringency and are more desirable than other types of persimmons. The PCNA/non-PCNA trait is qualitatively controlled by AST/ast alleles at a single locus. Persimmons are hexaploid, and PCNA of Japanese origin and non-PCNA genotypes are denoted as aaaaaa and A----, respectively, if AST gene denotes A and ast gene denotes a. 'Taiten' is an F-1, non-PCNA persimmon offspring with genotype AAaaaa derived from PCNA x non-PCNA, and molecular markers linked AST alleles in 'Taiten' shows polymorphism as A(2) and A(3), so that the genotype of 'Taiten' can show A(2)A(3)aaaa using the alleles of molecular marker. Offspring from a backcross of 'Taiten' x 'Kanshu' (PCNA) were evaluated. The astringency type was analyzed phenotypically, by fruit sensory and brown specks tests, and genotypically by SCAR markers with PCR primer pairs (E4/A2r for detecting the SCAR marker A(2) and 7H9F/AST-R for both A(2) and A(3)). The ratio of PCNA offspring was 43/251 (17.1%), and there was discrepancy between the phenotypic evaluation and SCAR marker estimation in only 3 offspring. The ratio was not significantly different from the theoretical ratio of nulliplex in autohexaploid. The PCNA offspring segregation ratio was 36/151 (23.8%) for 'Fuyu' (PCNA) x 'Taiten' and 6/29 (20.7%) for 'Tenjingosho' (PCNA) x 'Taiten' (nearly the same ratio reported for 'Taiten' x 'Kanshu'). Marker-assisted selection using SCAR markers is thus effective and practical in persimmon breeding. (C) 2014 Elsevier B.V. All rights reserved.
  • Cultivar Identification and Analysis of Genetic Relationships of Japanese Maples Using Simple Sequence Repeat (SSR) Markers, Kanzaki Shinya, Inoue Kouichi, Utsunomiya Naoki, Yano Masayoshi, Hort. Res. (Japan), Hort. Res. (Japan), 12(1), 1 - 7, 2013
    Summary:In this study, a set of seven simple sequence repeat (SSR) markers were developed for Japanese maple, and the genetic diversity of 107 Japanese maple cultivars was estimated using these markers. A total of 87 alleles were detected, ranging from 4 to 25 per locus, with an average of 12.43 alleles per locus. Most cultivars showed unique SSR profiles, indicating that the markers were effective for cultivar identification, while some pairs of bud-sport cultivars and synonyms showed identical SSR profiles. Principal component analysis (PCA) indicated that 107 Japanese maple cultivars were divided into two groups: one consists of cultivars of Acer palmatum, and the other group includes cultivars of two varieties, A. amoenum var. amoenum and A. amoenum var. matsumurae. Analysis of molecular variance (AMOVA) revealed significant differentiation between the two species, while the genetic differentiation and distance between the two varieties of A. amoenum were very low, with FST = 0.026 and D = 0.057, respectively. Thus, the two varieties of A. amoenum are genetically indistinguishable from each other in domesticated cultivars, while most cultivars of A. palmatum have been developed separately from those of A. amoenum.
  • Nursery-grafting Method for Persimmon Propagation Using a Current Rootstock, Wakisaka Masaru, Sugimura Teruhiko, Ishimori Tomoya, Kanzaki Shinya, Hort. Res. (Japan), Hort. Res. (Japan), 8(4), 427 - 432, 2009
    Summary:We present a new nursery grafting method for persimmon (Diospyros kaki Thunb.) propagation. This study examined the effects of grafting time and leaves of rootstock on the graft-take ratio and growth of grafted plants. Between April and September, we were able to graft a scion of 'Fuyu' persimmon onto a current seedling of 'Hourenbou' rootstock, which was between 110 and 195 days old. Although grafting onto a current seedling without leaves yielded a low graft-take ratio, 80 to 100% of scions grafted onto rootstock with leaves were established successfully. Between rootstock with and without leaves, a significant difference was noted in the callus-forming ratios 20 days after grafting. A callus was observed in all nursery plants grafted onto rootstock with leaves, irrespective of the bud-break feasibility.
  • Evaluation of RFLP analysis for discriminating PCNA genotype in some persimmon cultivars, S. Kanzaki, K. Yonemori, A. Sato, M. Yamada, A. Sugiura, Journal of the Japanese Society for Horticultural Science, Journal of the Japanese Society for Horticultural Science, 69, 702 - 704, Dec. 01 2000
    Summary:The cultivation of the PCNA (pollination-constant and non-astringent)-type persimmon is advantageous because of its natural loss of astringency on the tree. We previously found RFLP markers linked to the dominant alleles controlling the PCNA/non-PCNA trait and showed that the PCNA genotype could be distinguished from non-PCNA genotype by these markers in the breeding populations. In this study, RFLP markers were investigated with 33 persimmon cultivars. All PCNA cultivars except for Chinese 'Luo Tian Tian Shi', had identical banding pattern, whereas the non-PCNA cultivars exhibited many polymorphic bands. Thus, we could distinguish PCNA cultivars of Japanese origin from non-PCNA cultivars by the RFLP analysis, although 'Luo Tian Tian Shi' was mistakenly classified as a non-PCNA type. These results suggest that this marker system could be applied to distinguish PCNA cultivars of Japanese origin from non-PCNA ones.
  • RFLP Analysis of an Amplified Region of cpDNA for Phylogeny of the Genus Diospyros, YONEMORI Keizo, PARFITT Dan e, KANZAKI Shinya, SUGIURA Akira, UTSUNOMIYA Naoki, SUBHADRABANDHU Suranant, Journal of the Japanese Society for Horticultural Science, Journal of the Japanese Society for Horticultural Science, 64(4), 771 - 777, Mar. 15 1996
    Summary:RFLP analysis of PCR products from a variable region of the chloroplast genome was conducted to determine their potential for use in phylogenic studies of the genus <I>Diospyros</I>. The target region of cpDNA was reliably amplified by the procedure of Arnold et al. (1991) from all 14 Diospyros species tested. When restriction fragment polymorphisms of amplified products were examined at the intraspecific level with 10 endonucleases (<I>Ase</I> I, <I>Bfa</I> I, <I>Bst</I> NI, <I>Dde</I> I, <I>Msp</I> I, <I>Nco</I> I, <I>Rsa</I> I, <I>Scr</I> FI, <I>Sty</I> I, and <I>Taq</I> I), 15 cultivars of persimmon (<I>D. kaki</I>) and 3 horticultural varieties of <I>D. lotus</I> were monomorphic within species. However, restriction fragment polymorphisms were observed at the interspecific level. The 14 <I>Diospyros</I> species tested could be divided into 6 groups by the digestion patterns with ScrF I, 5 groups with <I>Bst</I> NI or <I>Taq</I> I, 4 groups with <I>Bfa</I> I or <I>Sty</I> I, 3 groups with <I>Ase</I> I, <I>Dde</I> I, or <I>Nco</I> I, 2 groups with <I>Msp</I> I or <I>Rsa</I> I. These results clearly indicated that the present analysis method is extremely valuable for phylogenetic and evolutionary studies at the interspecific level of the genus <I>Diospyros</I>.
  • The R2R3-MYB transcription factor MiMYB1 regulates light dependent red coloration of ‘Irwin’ mango fruit skin, Shinya Kanzaki, Asuka Ichihi, Yuta Tanaka, Shiina Fujishige, Sota Koeda, Kosuke Shimizu, Scientia Horticulturae, Scientia Horticulturae, 272, 109567 - 109567, Oct. 2020 , Refereed
  • Ty-2 and Ty-3a Conferred Resistance Are Insufficient against Tomato Yellow Leaf Curl Kanchanaburi Virus from Southeast Asia in Single or Mixed Infections of Tomato, Sota Koeda, Ikuya Fujiwara, Yuki Oka, Elly Kesumawati, Sabaruddin Zakaria, Shinya Kanzaki, Plant Disease, Plant Disease, Jun. 09 2020 , Refereed
  • Characterization of the Recombinant UDP:flavonoid 3-O-galactosyltransferase from Mangifera indica ‘Irwin’ (MiUFGalT3) involved in Skin Coloring, Ayako Katayama-Ikegami, Zion Byun, Suzuka Okada, Masahiro Miyashita, Takane Katayama, Tomoaki Sakamoto, Asuka Ichihi, Kosuke Shimizu, Shinya Kanzaki, The Horticulture Journal, The Horticulture Journal, 2020 , Refereed
  • Multiple Non-pungent Capsicum chinense Accessions with a Loss of Function CaKR1 Allele Originating from South America, Sota Koeda, Ryutaro Nakano, Takaya Sawaki, Kosuke Sato, Yoshiyuki Tanaka, Shinya Kanzaki, The Horticulture Journal, The Horticulture Journal, 2020 , Refereed
  • Pepper yellow leaf curl Aceh virus: a novel bipartite begomovirus isolated from chili pepper, tomato, and tobacco plants in Indonesia., Elly Kesumawati, Shoko Okabe, Kanami Homma, Ikuya Fujiwara, Sabaruddin Zakaria, Shinya Kanzaki, Sota Koeda, Archives of virology, Archives of virology, 164(9), 2379 - 2383, Sep. 2019 , Refereed
    Summary:During 2017, leaf samples of chili pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants exhibiting yellowing and curling symptoms were collected from Aceh province, Indonesia. These samples were used to isolate and sequence viral genomic DNA. Six isolates with complete DNA-A and DNA-B sequences of begomovirus were obtained, all of which showed >99% sequence identity to the others. DNA-A sequences shared the highest nucleotide sequence identity (89.3%-89.7%) with monopartite pepper yellow leaf curl Indonesia virus 2 (PepYLCIV2) and the second-highest sequence identity (87.3%-87.4%) with bipartite pepper yellow leaf curl Indonesia virus (PepYLCIV). The DNA-B sequences shared the highest nucleotide sequence identity (95%-97.5%) with PepYLCIV. Results of recombination analysis indicated that the novel begomovirus was a recombinant. In accordance with the guidelines for begomovirus species demarcation, these isolates should be assigned to a new species, and we have proposed the name ''pepper yellow leaf curl Aceh virus'' (PepYLCAV) for this virus.
  • Isolation of UDP:flavonoid 3-O-glycosyltransferase (UFGT)-like Genes and Expression Analysis of Genes Associated with Anthocyanin Accumulation in Mango ‘Irwin’ skin, Shinya Kanzaki, Shiori Kamikawa, Asuka Ichihi, Yuta Tanaka, Kosuke Shimizu, Sota Koeda, Naoki Utsunomiya, The Horticulture Journal, The Horticulture Journal, 88(4), 435 - 443, 2019 , Refereed
    Summary:<p>The red coloration of the mango 'Irwin' skin is an important factor determining its value in the Japanese domestic luxury fruit market. In the present study, to investigate the molecular mechanism underlying anthocyanin biosynthesis of mango fruit skin, UFGT-like genes were isolated and the expression profile of anthocyanin-related genes was determined. Several UFGT-like genes were identified in transcriptome data of red 'Irwin' mango skin and two genes, <i>MiUFGT1</i> and <i>MiUFGT3</i>, were considered to be involved in mango skin coloration. Deduced amino acid sequences of these genes exhibited high similarity to other plant UFGTs and contained the conserved PSPG box common to the glycosyltransferase family. The presence of a glutamine and a histidine residue at the C-terminus end of the PSPG box in <i>MiUFGT1</i> and <i>MiUFGT3</i>, respectively, implied that <i>MiUFGT1</i> and <i>MiUFGT3</i> use glucose and galactose, respectively, as a sugar donor; however, the actual function and sugar donor preference of these enzymes remain to be elucidated. Expression analysis of anthocyanin-related genes during skin coloration suggested that <i>MiCHS</i> and <i>MiANS</i>, as well as <i>MiUFGT1</i> and <i>MiUFGT3</i>, play important roles in the anthocyanin biosynthesis of mango fruit skin and that the expression of these genes is regulated by the MYB transcription factor, as reported in other plant species.</p>
  • Inoculation of Capsicums with Pepper Yellow Leaf Curl Indonesia Virus by Combining Agroinoculation and Grafting, Sota Koeda, Kanami Homma, Yuri Tanaka, Daiki Onizaki, Elly Kesumawati, Sabaruddin Zakaria, Shinya Kanzaki, The Horticulture Journal, The Horticulture Journal, 87(3), 364 - 371, 2018 , Refereed
    Summary:© 2018 The Japanese Society for Horticultural Science (JSHS), All rights reserved. Indonesia is one of the world’s largest fresh pepper (Capsicum spp.) fruit-producing countries, and hot peppers are essential spices in Indonesian cuisine. During the last two decades, begomovirus, which is transmitted by the whitefly, Bemisia tabaci (Gennadius), and causes pepper yellow leaf curl disease, began to cause a huge economic loss by damaging pepper plants in Indonesia. In the present study, a highly efficient inoculation method was established for Pepper yellow leaf curl Indonesia virus (PepYLCIV), the most infectious bipartite begomovirus in pepper plants cultivated in North Sumatra, by combining agroinoculation and subsequent grafting. Partial tandem repeats of PepYLCIV DNA A and B were constructed and cloned into a binary pGreenII vector, and their infectivity was tested. Co-inoculation of Nicotiana benthamiana L. and Solanum lycopersicum L. ‘Momotaro’ with PepYLCIV DNA A and DNA B resulted in the production of typical begomoviral symptoms. Both the injection of the cotyledons with cultured agrobacteria and the inoculation of the hypocotyl with agrobacterial colonies induced viral symptoms in pepper No. 218 (C. annuum L.) seedlings in approximately 55–75%. When agroinoculated symptomatic No. 218 was grafted onto an uninfected ‘Takanotsume’ (C. annuum), all newly elongated shoots from the rootstock of ‘Takanotsume’ produced typical begomoviral symptoms. Agroinoculation combined with subsequent grafting provides a highly efficient method for introducing PepYLCIV into pepper plants.
  • Highly Efficient Agroinoculation Method for Tomato Plants with Tomato Yellow Leaf Curl Kanchanaburi Virus, Sota Koeda, Kanami Homma, Yuri Tanaka, Elly Kesumawati, Sabaruddin Zakaria, Shinya Kanzaki, HORTICULTURE JOURNAL, HORTICULTURE JOURNAL, 86(4), 479 - 486, 2017 , Refereed
    Summary:Tomato yellow leaf curl disease caused by begomoviruses is a serious threat to tomato (Solanum lycopersicum L.) production. If begomoviruses, transmitted by whitefly (Bemisia tabaci), infect tomato plants during early growth, production can be almost entirely lost. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus, is emerging as an important threat to solanaceous crop production in Southeast Asia. The lack of mechanical transmission of some begomoviruses is a major experimental constraint. In this study, an agroinoculation method using TYLCKaV in tomato plants was established. Partial tandem repeats of TYLCKaV DNA A and DNA B were constructed and cloned to a binary pGreenII vector, and their infectivity was tested. Co-inoculation of TYLCKaV DNA A and DNA B to Nicotiana benthamiana L. produced typical begomoviral symptoms, and both of the viral DNA components accumulated in the upper uninoculated leaves, suggesting systemic infection of TYLCKaV. Two agroinoculation methods were conducted on tomatoes. First, excised sections of tomato shoots were agroinoculated with a soaking procedure. Although two Agrobacterium tumefaciens strains were tested, approximately 40% of inoculated plants only showed viral symptoms for EHA105. Second, agrobacterium from a cultured petri dish was directly inoculated with a colony inoculation procedure. When EHA105 was used, approximately 92% of inoculated plants showed viral symptoms. Sequencing the recovered viral DNA from the upper uninoculated leaf clarified that TYLCKaV had successfully infected the tomato plants. The colony inoculation procedure is labor-saving, and viral symptoms develop in susceptible tomato plants within approximately a month from sowing the seeds. This method could contribute to simple and speedy evaluation of TYLCKaV resistance of tomato plants.
  • The relationship between aroma component compositions of several citrus oils and the physio-psychological effects on humans, Chiaki Hara, Kenichi Tomi, Kosuke Shimizu, Shinya Kanzaki, Yutaka Tsuchihashi, Takahiro Hayashi, CHEMICAL SENSES, CHEMICAL SENSES, 41(9), E263 - E264, Nov. 2016 , Refereed
  • The Origin and Cultivar Development of Japanese Persimmon (Diospyros kaki Thunb.), Shinya Kanzaki, JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI, 63(7), 328 - 330, 2016
    Summary:Persimmon (Diospyros kaki Thunb.) is a hexaploid (2n = 6x = 90) that originated in southern China through polyploidization from a diploid ancestor. The exact ancestor of persimmon has not been clarified yet though much research has been conducted to elucidate it. Recent studies showed that D. glandulosa and D. oleifera have a close relationship with the diploid ancestor. Many persimmon cultivars have been developed through the long history of cultivation. Based on the nature of loss of astringency of the fruit, persimmon cultivars are classified into four types. There are two non-astringent types, PVNA and PCNA, and the mechanism of astringency loss is different between them. Furthermore, Chinese PCNA cultivar (C-PCNA) was proven to have a different physiological nature from Japanese PCNA cultivars. Though the gene conferring the PCNA or C-PCNA trait has not been identified yet, the nature of astringency loss has been gradually elucidated at the molecular level.
  • Relationships among Asian persimmon cultivars, astringent and non-astringent types, Dan E. Parfitt, Keizo Yonemori, Chitose Honsho, Mitsunori Nozaka, Shinya Kanzaki, Akihiko Sato, Masahiko Yamada, TREE GENETICS & GENOMES, TREE GENETICS & GENOMES, 11(2), Apr. 2015 , Refereed
    Summary:A molecular marker analysis for Asian persimmon cultivar relationships and pollination status was conducted with 496 amplified fragment length polymorphism (AFLP) markers and 146 cultivars of Asian origin. Cultivars from China, Korea, and Japan were evaluated for marker composition and pollination status, which strongly influences fruit characteristics. Separation of Chinese, Korean, and Japanese cultivar groups and pollination type by neighbor-joining clustering, multidimensional scaling, and STRUCTURE was only weakly supported and not genetically significant. Significant differences for cultivar origin and pollination status were found for analysis of molecular variance (AMOVA), but most of the variation was among cultivars, not classification groups. All of the cultivar groups were genetically similar at the molecular level with most polymorphism due to individual cultivar differences.
  • Application of Marker-assisted Selection in Persimmon Breeding of PCNA Offspring Using SCAR Markers among the Population from the Cross between Non-PCNA 'Taigetsu' and PCNA 'Kanshu', Nobuhito Mitani, Atsushi Kono, Masahiko Yamada, Akihiko Sato, Shozo Kobayashi, Yusuke Ban, Toshihito Ueno, Mikio Shiraishi, Shinya Kanzaki, Tomoyuki Tsujimoto, Keizo Yonemori, HORTSCIENCE, HORTSCIENCE, 49(9), 1132 - 1135, Sep. 2014
    Summary:Persimmon (Diospyros kaki Thunb) is hexaploid, and the pollination-constant, non-astringent (PCNA)/non-PCNA trait of Japanese origin is qualitatively controlled by the AST/ast alleles at a single locus and the PCNA trait is recessive to the non-PCNA trait. To avoid inbreeding depression led by repeated crosses among PCNA genotypes, non-PCNA genotypes should be used as cross parents. The marker-assisted selection system has been developed for the selection of PCNA offspring in the progeny derived from the cross of non-PCNA 'Taigetsu' (non-PCNA 'Kurokuma' X PCNA 'Taishu') to PCNA 'Kanshu'. The primer pairs E8.5/E9r and 7H9F/AST-R were used for detecting the molecular markers A(1) and A(3), respectively, which link AST alleles. Complete agreement was found between the sequence-characterized amplified region (SCAR) marker genotype and fruit astringency phenotype of the 48 offspring. The result confirmed that the marker-assisted selection using those markers was highly practical. In a larger offspring population (522 offspring) from the same cross, offspring segregated into 100 with both markers, 162 with only A(1), 179 with A(3), and 81 with neither, and this segregation ratio was significantly different from 2:3:3:2, which is the segregation ratio of random chromosome assortment in autohexaploid. The percentage of offspring expected to be PCNA was 15.5% (81 of 522), which was slightly lower than 20%.
  • Isolation and expression analysis of FLOWERING LOCUS T-like and gibberellin metabolism genes in biennial-bearing mango trees, Masahiro Nakagawa, Chitose Honsho, Shinya Kanzaki, Kousuke Shimizu, Naoki Utsunomiya, SCIENTIA HORTICULTURAE, SCIENTIA HORTICULTURAE, 139, 108 - 117, May 2012
    Summary:Mango (Mangifera indica L.) is one of the most important evergreen fruit trees, but it has a high tendency of biennial bearing because of irregular flowering. In this study, a FLOWERING LOCUS T-like (FT-like) gene was isolated from mango (MiFT) and characterized. The deduced amino acid sequence of MiFT showed high identity of the gene to other plant FT-like genes, and further, MiFT expression increased only in the leaves under floral-inductive conditions. Comparison of heavy crop load (HC) and no crop load (NC) mango trees showed that MiFT expression strongly increased only in NC trees under cool temperature. In NC trees, almost all the apical buds formed panicles, whereas in HC trees, only a few panicles were produced in the next season of fruit set. Further. HC trees had lower starch content in the shoots than NC trees. Furthermore, application of 250-ppm gibberellin 3 (GA(3)) completely inhibited flowering and MiFT expression in both HC and NC trees. GA metabolism genes were also isolated from mango and their expression patterns were investigated. Gibberellin-3-oxidase (GA3-ox) controls the final step of biosynthesis of active GA, and its gene expression surged only in HC trees under cool temperature. In conclusion, MiFT is considered a key factor in mango flowering, and regulation of MiFT expression through GA metabolism was discussed. (C) 2012 Elsevier B.V. All rights reserved.
  • New Candidate for the Ancestor of Japanese Persimmon (Diospyros kaki Thunb.) Found in Yunnan Province of China, K. Yonemori, M. Yamada, Zhengrong Luo, S. Kanzaki, Yong Yang, Renzi Wang, I INTERNATIONAL SYMPOSIUM ON WILD RELATIVES OF SUBTROPICAL AND TEMPERATE FRUIT AND NUT CROPS, I INTERNATIONAL SYMPOSIUM ON WILD RELATIVES OF SUBTROPICAL AND TEMPERATE FRUIT AND NUT CROPS, 948, 121 - 128, 2012 , Refereed
    Summary:There are few reports for the origin of Japanese persimmon (Diospyros kaki) and no species is defined as ancestor for it. However, so far, D. glandulosa (syn. D. roxburghii) was reported as a candidate for the origin of persimmon by Ng (Malaysian Forester 41:43-50, 1978), and D. oleifera and D. lotus are also reported to have very close relationship to D. kaki in phylogenetic analyses using the sequences of matK gene of the chloroplast DNA and ITS region of the genomic DNA by our research (Tree Genetics & Genomes 4: 149-158, 2008). These three candidate species are all diploid (2n=2x=30) in contrast that almost all of D. kaki is hexaploid (2n=6x=90). Recently, our survey of Diospyros species in China allowed us to find a new possible candidate of D. kaki, called "ye-mao-shi" in Chinese, in addition to these three candidate species. This new candidate seemed to be much closer to D. kaki than the previous three candidates due to morphological similarities of shape and size of leaf, fruit, and calyx. It was just like D. kaki in morphology, except that the fruit skin is pubescent in contrast with glabrous skin for D. kaki. The leaf surface of this new candidate is also a little bit pubescent. We found this candidate at Xishuangbanna Tropical Botanic Garden in Yunnan Province. According to the survey of specimens at the herbariums of Xishuangbanna Tropical Botanic Garden, Kunming Botanical Garden, Wuhan Botanical Garden, and Beijing Botanical Garden in China, this candidate was confused in the classification and was categorized into both D. kaki var. sylvestris and D. kaki. In fact, there were two types classified into D. kaki var. sylvestris and D. kaki; one has pubescent fruit skin and the other has glabrous fruit skin. The candidate having pubescent fruit skin, called "ye-mao-shi", should be classified as different categorization from D. kaki or D. kaki var. sylvestris. Interestingly, according to our herbarium survey in China, almost all of this candidate was distributed preferentially in Yunnan Province of China. Now, we are trying to analyze this new candidate to validate the genetic relationship to D. kaki in phylogenetic trees. This candidate should be closer to D. kaki among the species we analyzed and we are now planning to analyze the phylogenetic position of this new candidate among other wild Diospyros species we collected.
  • Efficiency of Hybrid Formation by Open-pollination of Two Cultivars in a Closed Plastic House and the Effect of the Male Parent on Fruit Characteristics in Mango, Chitose Honsho, Marie Inada, Ken-ichi Yuji, Masahiro Tojiki, Shigefumi Kurogi, Shinya Kanzaki, Takuya Tetsumura, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 81(1), 27 - 34, Jan. 2012
    Summary:Although Japanese mango is produced under a fairly unique cultivation system and fetches high prices at market, further growth of the mango industry in Japan requires the development of new cultivars. However, since individual mango flowers are quite small, no more than 1 cm in diameter, a large number of skilled workers is necessary for artificial pollination, thus making it difficult to obtain a sufficient number of progenies for breeding. We therefore evaluated a methodology whereby progenies were obtained by open pollination and their male parent was subsequently determined by DNA markers. Two mango cultivars ('Irwin' and 'Beni-Keitt') were arranged in a plastic house and honeybees were released as a pollen vector for open pollination. Harvested fruits were characterized and their seeds were sown in a bed. The male parent of the germinated seedlings was then identified by five simple sequence repeat (SSR) markers. As a result of SSR genotyping, the male parents of 185 of 239 'Irwin' seedlings were revealed; 79 were obtained by self-pollination and 106 from out-crossing. For 'Beni-Keitt', the male parent of 14 of the 20 seedlings was determined with two self-pollinated and 12 out-crossed. Preferential out-crossing in 'Irwin' was revealed using the chi-square test, although the considerable number of self-pollinated fruit obtained shows that a sufficient number of fruit can be set in a single-planted orchard. The effect of the male parent on fruit characteristics was subsequently examined, revealing that in 'Irwin' the Brix value of self-pollinated fruits was significantly higher than that of cross-pollinated fruits. Certain color values were, however, lower in self-pollinated 'Irwin' fruits.
  • Development of Molecular Markers Linked to the Allele Associated with the Non-astringent Trait of the Chinese Persimmon (Diospyros kaki Thunb.), Ayako Ikegami, Sai Eguchi, Takashi Akagi, Akihiko Sato, Masahiko Yamada, Shinya Kanzaki, Akira Kitajima, Keizo Yonemori, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 80(2), 150 - 155, Apr. 2011
    Summary:Persimmon cultivars are classified into four types depending on the nature of astringency loss of the fruit, namely pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA), pollination variant astringent (PVA), and pollination constant astringent (PCA). Among these four types, PCNA is the most important cultivar for persimmon breeding due to the stable loss of natural astringency on the tree. The trait of natural astringency loss is recessive in Japanese PCNA cultivars, while that in the Chinese PCNA cultivar, 'Luo tian tian shi', is dominant and the latter locus, termed CPCNA, is different from that in Japanese cultivars. In order to develop a molecular marker for the selection of the CPCNA-type cultivar, we performed amplified fragment length polymorphism (AFLP) in combination with bulked segregant analysis for F-1 offspring derived from 'Luo tian tian shi', which was used as the maternal parent. A total of 384 primer combinations were tested, and three AFLP markers, namely EACT-MCCC-222 (RO1), EGGC-MCTC-309 (RO2), and EGCC-MCGA-105 (RO3), linked to the CPCNA dominant allele were obtained. Among these markers, EGGC/MCTC-309 (RO2) was converted into a sequence-characterized amplified region (SCAR) marker. PCR analysis using F-1 offspring (n = 264) revealed that the relevance ratio of the SCAR marker was 94%. The polymorphism of the RO2 marker, which was strongly linked to the CPCNA dominant allele, was detected in only two Chinese PCNA cultivars, namely 'Luo tian tian shi' and 'Tian bao gai', among the Chinese, Korean, and Japanese cultivars tested. These results indicate that the RO2 marker contributes to marker-assisted selection for breeding programs of new PCNA cultivars having the CPCNA trait.
  • SCAR Markers for Practical Application of Marker-assisted Selection in Persimmon (Diospyros kaki Thunb.) Breeding, Shinya Kanzaki, Takashi Akagi, Takuya Masuko, Miyuki Kimura, Masahiko Yamada, Akihiko Sato, Nobuhito Mitani, Naoki Ustunomiya, Keizo Yonemori, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 79(2), 150 - 155, Apr. 2010
    Summary:In previous studies, we have developed molecular markers linked to the AST locus that controls fruit astringency type in persimmon; however, these markers are not feasible for practical application to breeding programs since they are not fully effective for discriminating the pollination constant and non-astringent (PCNA) genotype from the non-PCNA genotype in a progeny derived from 'Kurokuma'. Here we developed new sequence characterized amplified region (SCAR) markers that enable easy and reliable selection of the PCNA genotype from breeding populations. Genomic regions adjacent to probe 5R, which showed polymorphic fragments between PCNA and non-PCNA genotypes in restriction fragment length polymorphism (RFLP) analysis, were isolated from the genomic libraries of 'Nishimura-wase', 'Jiro', and 'Kurokuma'-derived offspring. The isolated genomic regions were characterized and 3 insertion/deletion mutations were observed between ast- and AST-linked regions. Several primers were designed in the flanking region of Indel-3 and, in multiplex PCR, it was shown that using 2 forward primers, AST-F and PCNA-F and a reverse primer, 5R3R, is the most useful and reliable primer set. The AST-linked 220-bp fragment proved to be a common marker of 'Kurokuma'-, 'Nisimura-wase'- and 'Aizumishirazu'- derived progenies. This multiplex PCR is considered the most practical tool for marker-assisted selection (MAS) and can enhance and accelerate progress in persimmon breeding.
  • Quantitative Genotyping for the Astringency Locus in Hexaploid Persimmon Cultivars using Quantitative Real-time PCR, Takashi Akagi, Yumi Takeda, Keizo Yonemori, Ayako Ikegami, Atsushi Kono, Masahiko Yamada, Shinya Kanzaki, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 135(1), 59 - 66, Jan. 2010
    Summary:Persimmon (Diospyros kaki Thunb.) is generally hexaploid, and a single AST locus controls the pollination-constant non-astringency trait on each of six corresponding chromosomes. The pollination-constant non-astringent (PCNA) genotype is nulliplex and requires homozygous recessive alleles (ast) at the AST locus. There are several non-PCNA cultivars/selections that could be cross parents; however, the probability of yielding nulliplex offspring depends on the number of recessive alleles (ast). In genotyping for the AST locus in hexaploid persimmon, in contrast to the situation in diploid plants, we need to detect the AST/ast allele dosage; this cannot be detected by common codominant markers. In this study, we detected the allele dosage of M(ast), which is a marker allele strongly linked to the ast allele among cultivars, by quantitative real-time polymerase chain reaction (qPCR) using three reference sites, actin (DkAct), anthocyanin reductase (DkANR), and L5R, whose sequences are conserved in the genome of persimmon cultivars. Based on the allele dosage of the M(ast), AST/ast genotypes were estimated for 63 non-astringent cultivars/selections, of which only five cultivars/selections were estimated to be simplex or duplex. The quantitative genotyping method using qPCR may be generally effective for polyploid plants.
  • Quantitative real-time PCR to determine allele number for the astringency locus by analysis of a linked marker in Diospyros kaki Thunb, Takashi Akagi, Shinya Kanzaki, Mai Gao, Ryutaro Tao, Dan E. Parfitt, Keizo Yonemori, TREE GENETICS & GENOMES, TREE GENETICS & GENOMES, 5(3), 483 - 492, Jul. 2009
    Summary:Persimmon (Diospyros kaki Thunb.) is a polyploidy fruit tree species of economic importance to East Asia. Natural astringency loss is an important trait in persimmon breeding programs. Quantitative real-time PCR was used to determine the number of AST/ast alleles for fruit astringency in persimmon (D. kaki Thunb.). To this end, the cultivar Jiro was transformed with one or two copies of a gene encoding NADP-dependent sorbitol-6-phosphate dehydrogenase (S6PDH), which was used as a standard for measuring the allele number of a sequenced marker tightly linked to the recessive ast locus for nonastringency. Primers for markers linked to the AST or ast allele were then used to measure the AST to ast ratio directly in the progeny of a full-sib cross. From determination of the AST to ast ratio and the results of the S6PDH copy number, the number of AST and ast alleles at the AST/ast locus was estimated. This research supported the hypothesis that D. kaki is a hexaploid with six AST and/or ast alleles. In addition to the determination of the allelic status of the AST locus, the application of real-time PCR for confirmation of the ploidy level and allelic composition of target genes in autopolyploids or allopolyploids was demonstrated.
  • Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb.) into PCR-Based Markers, Shinya Kanzaki, Masahiko Yamada, Akihiko Sato, Nobuhito Mitani, Naoki Ustunomiya, Keizo Yonemori, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 78(1), 68 - 73, Jan. 2009 , Refereed
    Summary:Persimmon (Diospyros kaki Thunb.) cultivars are classified into 4 types depending on the relationship between astringency of the mature fruit and the effect of seeds on the loss of astringency, and only pollination-constant and non-astringent (PCNA)-type persimmons stably lose fruit astringency as a part of fruit development. This is a recessive trait, regarded to be controlled by a single locus, namely, the AST locus, which has a polysomic nature. Thus far, we have identified 2 restriction fragment length polymorphism (RFLP) markers, namely, A1 and A2, each of which is separately linked to a different AST allele, and proved that the RFLP markers were useful for selecting PCNA-type persimmons. This study was conducted to convert the RFLP markers into polymerase chain reaction (PCR)-based markers. We isolated and characterized genomic regions corresponding to each RFLP marker by inverse PCR. Two primer pairs, E4/E9r and E4/A2r, were designed to generate 2 sequence characterized amplified region (SCAR) markers, i.e., PCR-A1 and PCR-A2, respectively. The PCR-A1 and PCR-A2 markers cosegregated with the A] and A2 markers, respectively, in 'Nishimura-wase'-derived progenies. Although the primer pair E4/A2r did not produce the PCR-A2 marker in the FU-275, which is a progeny derived from 'Aizumishirazu', all non-PCNA-type offspring but no PCNA-type offspring showed the PCR-A1 marker using the primer pair E4/E9r. Thus, it was revealed that the SCAR markers were useful for selecting PCNA-type offspring in these progenies. On the other hand, disruption of the relationship between the markers and the AST locus was observed in the KU-325, derived from 'Kurokuma', indicating that the selection of PCNA-type offspring using PCR-based markers is not effective for progeny derived from 'Kurokuma'. Herein, we discuss the possibility of applying PCR-based markers to genetic studies.
  • Sequence analyses of the ITS regions and the matK gene for determining phylogenetic relationships of Diospyros kaki (persimmon) with other wild Diospyros (Ebenaceae) species, Keizo Yonemori, Chitose Honsho, Shinya Kanzaki, Hitofumi Ino, Ayako Ikegami, Akira Kitajima, Akira Sugiura, Dan E. Parfitt, TREE GENETICS & GENOMES, TREE GENETICS & GENOMES, 4(2), 149 - 158, Apr. 2008
    Summary:To elucidate the relationships among Diospyros kaki and species closely related in previous studies, the nuclear ribosomal internal transcribed spacer (ITS) DNA sequence and the chloroplast matK gene were sequenced and compared with those of nine Diospyros species from Thailand, four species from temperate regions, and one species of southern Africa, D. lycioides. Maximum parsimony, maximum likelihood, and neighbor joining analyses of the matK and ITS data sets revealed that D. kaki is closely related to two diploid species, D. oleifera and D. glandulosa. D. kaki, D. glandulosa, and D. oleifera were placed differently in the trees obtained from ITS and matK data sets, suggesting that hybridization and/or introgression may have occurred during the development of these species. D. kaki was not found to be closely related to D. ehretioides, a diploid species from Thailand. These results differed from a prior analysis of this genus performed with chloroplast DNA ( cpDNA) restriction site mutations in 3.2- and 2.1-kb amplified sequences. The results supported Ng's hypothesis that D. glandulosa and D. kaki may share a common ancestor. D. oleifera was also closely associated with D. kaki.
  • RFLP markers for the selection of pollination-constant and non-astringent (PCNA)-type persimmon and examination of the inheritance mode of the markers, Shinya Kanzaki, Akihiko Sato, Masahiko Yamada, Naoki Utsunomiya, Akira Kitajima, Ayako Ikegami, Keizo Yonemori, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 77(1), 28 - 32, Jan. 2008 , Refereed
    Summary:We investigated marker-assisted selection of the pollination constant and non-astringent (PCNA) trait and inheritance modes of the marker locus in persimmon (Diospyros kaki Thunb.) using restriction fragment length polymorphism (RFLP) analysis. In two backcross progenies, FU-170 and FU-275, 5 of 71 and 23 of 101 offspring, respectively, were judged as PCNA genotype by the marker phenotype. The astringency type of individual offspring was determined by measuring the size of tannin cells and soluble tannin content in mature fruit, thus confirming complete co-segregation of RFLP markers and the non-PCNA phenotype in 32 and 68 fruit-obtainable offspring from FU-170 and FU-275, respectively; therefore, RFLP markers can be used for PCNA genotype detection. Their segregation ratio deviated significantly from that of disomic inheritance. We also discuss the possibility of polysomic inheritance of RFLP markers.
  • Phylogenetic relationships of Mangifera species revealed by ITS sequences of nuclear ribosomal DNA and a possibility of their hybrid origin, K Yonemori, C Honsho, S Kanzaki, W Eiadthong, A Sugiura, PLANT SYSTEMATICS AND EVOLUTION, PLANT SYSTEMATICS AND EVOLUTION, 231(1-4), 59 - 75, 2002
    Summary:The phylogenetic relationships among 14 Mangifera L. species of Thailand were analyzed by comparing sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA). Parsimony and neighbor joining (NJ) analyses revealed that the common mango (M. indica L.) was closely related to M. laurina Bl., M. sylvatica Roxb., and Al. oblong folia Hook. f. Mangifera foetida Lour. and M. odorata Griff. were also related to M. indica in both parsimonious and NJ trees, although these two species are classified into a different subgenus (subgenus Limits) from the subgenus Mangifera to which M. indica belongs. ITS sequence analysis revealed that several species have nucleotide additivity (two different nucleotides at the same locus) at several sites in the ITS region. Also, M. indica had several polymorphisms among cultivars. This finding may suggest a possibility of hybrid origin of Mangifera species, although Mangifera species are all assumed to be diploid having chromosome number of 2n = 2x = 40.
  • Analysis of the genetic relationships among pollination-constant and non-astringent (PCNA) cultivars of persimmon (Diospyros kaki Thunb.) from Japan and China using amplified fragment length polymorphism (AFLP), S Kanzaki, K Yonemori, A Sato, M Yamada, A Sugiura, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 69(6), 665 - 670, Nov. 2000
    Summary:The genetic relationships among 19 pollination- constant and non- astringent (PCNA) cultivars and 14 non-PCNA cultivars of persimmon (Diospyros kaki Thunb.), including one Chinese PCNA cultivar 'Luo Tian Tian Shi', were analyzed by comparing 138 AFLP markers. A phylogenetic tree constructed based on the similarity indices of these AFLP markers indicated a close relationship among Japanese PCNA cultivars, but a more distant relationship with the Chinese PCNA cultivar 'Luo Tian Tian Shi'. The close relationship between PCNA cultivars native to Gifu prefecture was distinct, indicating that these cultivars developed from crosses among restricted sources in this region. The cultivars 'Gosho', 'Hana-gosho', 'Oo-gosho', and 'Yamato- gosho' showed a close relationship with some non-PCNA cultivars.
  • Amplified fragment length polymorphism analysis for studying genetic relationships among Mangifera species in Thailand, W Eiadthong, K Yonemori, S Kanzaki, A Sugiura, N Utsunomiya, S Subhadrabandhu, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 125(2), 160 - 164, Mar. 2000
    Summary:The phylogenetic relationships among 14 Mangifera L. species including three economically important species, i.e., common mango (M, indica L,), horse mango (M. foetida Lour.) and kwini (M. odorata Griff,), were analyzed by comparing 217 amplified fragment length polymorphism (AFLP) markers. The unweighted pair grouping method using arithmetic averages (UPGMA) and neighbor-joining (NJ) method were used and two outgroup tare, cashew nut (Anacardium occidentale L,) and gandaria (Bouea macrophylla Griff.), were added to both analyses. The common mango was closely related to banana mango (M. sylvatica Roxb.), M. laurina Bl., and M. oblongifolia Hook.f. Intraspecific variation among seven cultivars of common mango was much smaller than interspecific variation and these cultivars were classified into one M, indica group using both methods. Mangifera macrocarpa Bl., M. foetida, and M. odorata were also related to M. indica in both UPGMA and NJ trees, although these three species are classified into a different subgenus (subgenus Limus) from the subgenus Mangifera to which M. indica belongs. Also, in both UPGMA and NJ trees, M. gedebe Mig. and M. griffithii Hk.f. were placed in distant positions among the Mangifera species tested, indicating these two species are related distantly to M. indica. The AFLP technique was confirmed to be useful for phylogenetic analysis.
  • Phylogenetic relationships of the common durian (Durio zibethinus Murray) to other edible fruited Durio spp. by RFLP analysis of an amplified region of cpDNA, S Kanzaki, K Yonemori, A Sugiura, S Subhadrabandhu, JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 73(3), 317 - 321, May 1998 , Refereed
    Summary:Phylogenetic relationships among seven species of the genus Durio, of which four produce edible fruits including the common durian (D. zibethinus), were investigated from RFLP analysis of PCR products from a variable region of cpDNA. The target region of cpDNA was reliably amplified from all Durio species tested, using total DNAs extracted by a modified CTAB method of Doyle and Doyle (1987) followed by an additional purification step. When restriction fragment polymorphisms of amplified products were examined with 19 restriction endonucleases (AseI, BamHI, BfaI, DraI, EcoRI, EcoRV, HindIII, KpnI, MspI, RsaI, ScaI, ScrFI, SalI, SmaI, SspI, StyI, TaqI, XbaI, XhoI), RFLPs were observed with 14 endonucleases. However, since all Durio species tested had length mutation on this amplified region of cpDNA, the most parts of diversities from 14 endonucleases were caused by the length mutations among species. Considering length mutation among species, a total of seven site mutations were detected as well as 12 length mutations, based on D. zibethinus. When unordered parsimony analysis was conducted using these data, the cultivated durian (D. zibethinus) did not show any close relationships to other three species producing edible fruits. D. zibethimus formed a cluster with wild species, D. griffthii, and other three species made two clusters with D. lowianus or D. mansoni. Our results indicated an independent evolutionary process among four edible species of the genus Durio.
  • Phylogenetic relationships of the common durian (Durio zibethinus Murray) to other edible fruited Durio spp. by RFLP analysis of an amplified region of cpDNA, Shinya Kanzaki, Keizo Yonemori, Akira Sugiura, Suranant Subhadrabandhu, Journal of Horticultural Science and Biotechnology, Journal of Horticultural Science and Biotechnology, 73(3), 317 - 321, 1998
    Summary:Phylogenetic relationships among seven species of the genus Durio, of which four produce edible fruits including the common durian (D. zibethinus), were investigated from RFLP analysis of PCR products from a variable region of cpDNA. The target region of cpDNA was reliably amplified from all Durio species tested, using total DNAs extracted by a modified CTAB method of Doyle and Doyle (1987) followed by an additional purification step. When restriction fragment polymorphisms of amplified products were examined with 19 restriction endonucleases (AseI, BamHI, BraI DraI, EcoRI, EcoRV, HindIII, KpnI, MspI, RsaI, ScaI, ScrFI, SalI, SmaI, SspI, StyI, TaqI, XbaI, XhoI), RFLPs were observed with 14 endonucleases. However, since all Durio species tested had length mutation on this amplified region of cpDNA, the most parts of diversities from 14 endonucleases were caused by the length mutations among species. Considering length mutation among species, a total of seven site mutations were detected as well as 12 length mutations, based on D. zibethinus. When unordered parsimony analysis was conducted using these data, the cultivated durian (D. zibethinus) did not show any close relationships to other three species producing edible fruits. D. zibethinus formed a cluster with wild species, D. griffithii, and other three species made two clusters with D. lowianus or D. mansoni. Our results indicated an independent evolutionary process among four edible species of the genus Durio.
  • Diospyros species in Thailand: Their distribution, fruit morphology and uses, Naoki Utsunomiya, Suranant Subhadrabandhu, Keizo Yonemori, Masayoshi Oshida, Shinya Kanzaki, Fumiaki Nakatsubo, Akira Sugiura, Economic Botany, Economic Botany, 52(4), 343 - 351, 1998
    Summary:Diospyros species distributed widely in Thailand were classified into four ecotypes, according to their habitat constantly humid area, alternately dry and wet area, mountainous cool area and all area. Some of them inhabit near dwelling areas or in the paddy field in the village. The young fruit is covered with dense pubescence in most species. The size, shape, and color of mature fruit greatly vary greatly with the species. In most species, the mature fruit has a soft pulp and hard skin. The fruit of six species has been used for dying. Four species produce edible fruits, with color and flavor favorable for breeding of edible Diospyros species. The fruit of some species contains some chemicals useful as fish poisoning or of medicines, although the active components have not yet been identified. The edible fruit contained many tannin cells, but the fruit used as fish poisoning and medicines had only a few.
  • Phylogenetic relationships between the jackfruit, the breadfruit and nine other Artocarpus spp. from RFLP analysis of an amplified region of cpDNA, S Kanzaki, K Yonemori, A Sugiura, S Subhadrabandhu, SCIENTIA HORTICULTURAE, SCIENTIA HORTICULTURAE, 70(1), 57 - 66, Jun. 1997
    Summary:The phylogenetic relationships among eleven Artocarpus species, including two economically important species, the jackfruit (A. heterophyllus Lamk) and the breadfruit (A. altilis Fosberg), were estimated by comparing 30 restriction-site mutations in an amplified region of chloroplast DNA (cpDNA). Neighbor-joining and parsimony analyses were performed on the data to identify the relationships. The jackfruit and chempedak (A. integer Merr.) were monomorphic and therefore indistinguishable for all restriction sites. This confirmed the close relationship between these two species. The breadfruit and A. elasticus formed a monophyletic group with strong support from both neighbor-joining and parsimony analyses, indicating the possibility that the breadfruit was derived from A, elasticus or its close relatives. In general, our results were in agreement with the conventional classifications in Artocarpus species except for the position of A. chaplnsha. (C) 1997 Elsevier Science B.V.
  • RFLP analysis of an amplified region of cpDNA for phylogeny of the genus Diospyros, K Yonemori, DE Parfitt, S Kanzaki, A Sugiura, N Utsunomiya, S Subhadrabandhu, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 64(4), 771 - 777, Mar. 1996 , Refereed
    Summary:RFLP analysis of PCR products from a variable region of the chloroplast genome was conducted to determine their potential for use in phylogenic studies of the genus Diospyros. The target region of cpDNA. was reliably amplified by the procedure of Arnold et al. (1991) from all 14 Diospyros species tested. When restriction fragment polymorphisms of amplified products were examined at the intraspecific level with 10 endonucleases (Ase I, Bfa I, Bst NI, Dde I, Msp I, Nco I, Rsa I, Scr FI, Sty I, and Taq I), 15 cultivars of persimmon (D. kaki) and 3 horticultural varieties of D. lotus were monomorphic within species. However, restriction fragment polymorphisms were observed at the interspecific level. The 14 Diospyros species tested could be divided into 6 groups by the digestion patterns with Scrf I, 5 groups with Bst NI or Taq I, 4 groups with Bfa I or Sty I, 3 groups with Ase I, Dde I, or Nco I, 2 groups with Msp I or Rsa I. These results clearly indicated that the present analysis method is extremely valuable for phylogenetic and evolutionary studies at the interspecific level of the genus Diospyros.

Conference Activities & Talks

  • 異なる非辛味化因子を有するトウガラシ(Capsicum chinense)品種における果実の揮発性成分分析, 蓮真海, 尾崎早也佳, 大野公輝, 木下万智子, 富研一, 田中義行, 神崎真哉, 神崎真哉, 小枝壮太, 小枝壮太, 園芸学研究 別冊,   2018 09 22
  • 新たに見出した組換え型PepYLCIVの感染性クローンを用いたベンサミアナタバコへの接種, 岡部祥子, KESUMAWATI Elly, ZAKARIA Sabaruddin, 神崎真哉, 小枝壮太, 園芸学研究 別冊,   2018 09 22
  • Ty‐3a抵抗性トマト品種におけるTYLCKaVの増殖およびウイルスゲノムのメチル化, 藤原郁也, KESUMAWATI Elly, ZAKARIA Sabaruddin, 神崎真哉, 神崎真哉, 小枝壮太, 小枝壮太, 園芸学研究 別冊,   2018 09 22
  • マンゴー‘愛紅’における交互結実栽培の有効性, 清水恒介, 巽賢太郎, 神崎真哉, 宇都宮直樹, 園芸学研究 別冊,   2018 09 22
  • マンゴーの花成誘導因子MiFTの発現量および発現時期が花芽形成に及ぼす影響, 巽賢太郎, 小枝壮太, 志水恒介, 神崎真哉, 園芸学研究 別冊,   2018 09 22
  • マンゴーの果皮着色に関与する転写制御因子の機能解析, 一樋明日香, 小枝壮太, 志水恒介, 神崎真哉, 園芸学研究 別冊,   2018 09 22
  • マンゴー品種間における果実着色機構の差異, 神崎真哉, 田中祐太, 一樋明日香, 山本雄介, 小枝壮太, 志水恒介, 園芸学研究 別冊,   2018 03 24
  • インドネシアの園芸作物における黄化葉巻病に関連するベゴモウイルスの同定, KESUMAWATI Elly, 岡部祥子, 本間鹿波, 藤原郁也, ZAKARIA Sabaruddin, 神崎真哉, 小枝壮太, 園芸学研究 別冊,   2018 03 24
  • Ty‐3a抵抗性トマト品種におけるTYLCKaVおよびPepYLCIVの増殖, 藤原郁也, KESUMAWATI Elly, ZAKARIA Sabaruddin, 神崎真哉, 小枝壮太, 園芸学研究 別冊,   2018 03 24
  • 収穫時期の異なるマンゴー品種間における花成及び結実特性の違い, 巽賢太郎, 志水恒介, 宇都宮直樹, 神崎真哉, 熱帯農業研究,   2017 10 21
  • ベトナム北部山間農村に分布するカキ属植物の探索, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, SATO AKIHIKO, KITAJIMA AKIRA, HOA TRAN THI, YONEMORI KEIZO, 園芸学研究 別冊,   2015 03 28
  • マンゴー果皮におけるMYB転写因子の単離と発現解析, HYODO YOSHIHIRO, KANZAKI SHIN'YA, SHIMIZU KOSUKE, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2014 09 27
  • カンキツ類果実における香気成分の同定と品種間差異, KAMATANI MASAAKI, TOMI KEN'ICHI, KANZAKI SHIN'YA, SHIMIZU KOSUKE, FUMURO MASAHIKO, HAYASHI TAKAHIRO, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2014 03 29
  • マンゴーの果実および葉に含まれる香気成分の同定と品種間差異, OKAZAKI TOSHIKAZU, KANZAKI SHIN'YA, TOMI KEN'ICHI, HAYASHI TAKAHIRO, SHIMIZU KOSUKE, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2013 09 20
  • マンゴーの萌芽時における花成関連遺伝子の発現解析, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, HONSHO CHITOSE, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2011 09 24
  • マンゴー果実における香気成分の品種間差異, TSUJI TOSHIHIKO, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 熱帯農業研究,   2011 03 28
  • 晩生品種‘Sensation’を用いたマンゴーの抑制栽培技術の検討, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 熱帯農業研究,   2011 03 28
  • カキの甘渋性識別SCARマーカーを利用した完全甘ガキの選抜, MITANI NORIHITO, KONO ATSUSHI, YAMADA MASAHIKO, KANZAKI SHIN'YA, SATO AKIHIKO, KOBAYASHI SHOZO, BAN YUSUKE, UENO TOSHIHITO, SHIRAISHI MIKIO, AKAGI TSUYOSHI, TSUJIMOTO TOMOYUKI, YONEMORI KEIZO, 園芸学研究 別冊,   2011 03 20
  • マンゴー‘Irwin’の花成関連遺伝子の発現に及ぼす温度の影響, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, HONSHO CHITOSE, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2011 03 20
  • マンゴーの花成誘導期間中におけるジベレリン代謝関連遺伝子の発現解析, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 熱帯農業研究,   2010 10 09
  • カキの遺伝学的解析におけるマメガキの有用性, TSUJIMOTO TAKAYUKI, AKAGI TAKEJI, KANZAKI SHIN'YA, YONEMORI KEIZO, 園芸学研究 別冊,   2010 09 19
  • マンゴーの果肉崩壊症に及ぼす嫌気呼吸活性の影響, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 熱帯農業研究,   2010 03 27
  • 光質と窒素施肥量がハーブの香気ケモタイプに及ぼす影響, HAYASHI TAKAHIRO, NAKAMURA MINAKO, YOSHIOKA YUMI, NAKAGAWA MASAHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, SASAOKA MOTONOBU, ICHINOKI MUNEJI, YOSHIMI TAKANORI, SHIMADA TOMOHIRO, TOMI KEN'ICHI, MATSUMURA YASUKI, 園芸学研究 別冊,   2010 03 21
  • Effect of sugar concentrations, temperature and light on the anthocyanin formation in the flesh of blood orange, UTSUNOMIYA NAOKI, SAKAMOTO AKI, KANZAKI SHIN'YA, 園芸学研究 別冊,   2009 09 26
  • イロハカエデのSSRマーカーの開発, KANZAKI SHIN'YA, INOUE KOICHI, UTSUNOMIYA NAOKI, YANO MASAYOSHI, 園芸学研究 別冊,   2009 09 26
  • 1‐MCPおよびプロピオン酸カルシウム処理がマンゴー‘愛紅’果実の軟化に及ぼす影響, NAKAGAWA MASAHIRO, SHIMIZU KOSUKE, KANZAKI SHIN'YA, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, 熱帯農業研究,   2009 03 27
  • マンゴー‘アーウィン’および‘愛紅’の取り木発根向上のための処理方法, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, SASAKI KATSUAKI, SHIMIZU KOSUKE, KANZAKI SHIN'YA, 園芸学研究 別冊,   2009 03 19
  • カキの落葉時葉色の品種・系統間差異と紅葉生産に適した品種・系統の選抜, YAMADA MASAHIKO, KOGA YOSUKE, HIGA KOJI, YOKOYAMA TAKAMITSU, KOBAYASHI SHOZO, KONO ATSUSHI, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 園芸学研究 別冊,   2009 03 19
  • マンゴー‘アーウィン’,‘愛紅’および台湾在来種の取り木発根に及ぼすIBAとNAAの影響, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, SASAKI KATSUAKI, SHIMIZU KOSUKE, KANZAKI SHIN'YA, 園芸学研究 別冊,   2008 09 27
  • ドラゴンフルーツにおける葉状茎の生長に及ぼす植物生長調節物質の影響, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, SASAKI KATSUAKI, SHIMIZU KOSUKE, KANZAKI SHIN'YA, 園芸学研究 別冊,   2008 03 28
  • Effect of temperature on the anthocyanin formation in blood orange fruit after harvesting, HASHIMOTO H, SAKAMOTO A, KANZAKI S, UTSUNOMIYA N, FUMURO M, SHIMIZU K, 園芸学研究. 別冊, 園芸学会大会研究発表要旨,   2007 09 29
  • Effect of temperature on the development of flower and vegetative buds in Japanese apricot 'Nankho', TAKAMATSU Y, OTSUKA M, KANZAKI S, UTSUNOMIYA N, 園芸学研究. 別冊, 園芸学会大会研究発表要旨,   2007 09 29
  • Phylogenetic analysis of Japanese pepper distributed in the Kinki district, KANZAKI S, HARADA T, NAKAJIMA A, SAKO Y, UTSUNOMIYA N, 園芸学研究. 別冊, 園芸学会大会研究発表要旨,   2007 03 24
  • Segregation of fruit astringency type and selection of PCNA offspring using RFLP marker in the BC_1 progeny derived from 'Kurokuma' persimmon, KANZAKI S, KIMURA M, YAMADA M, MITANI N, AKAGI T, UTSUNOMIYA N, YONEMORI K, 園芸学研究. 別冊, 園芸学会大会研究発表要旨,   2007 03 24
  • Effect of Grafting Season and Presence of Leaves in Stock on Growth of Grafted Nursery Plant of Persimmon, WAKISAKA M, SUGIMURA T, KANZAKI S, 園芸学研究. 別冊, 園芸学会大会研究発表要旨,   2007 03 24
  • ドラゴンフルーツにおける果実形質間の相関関係およびGA3とCPPUが果実生長に及ぼす影響, FUMURO MASAHIKO, UTSUNOMIYA NAOKI, SASAKI KATSUAKI, SHIMIZU KOSUKE, KANZAKI SHIN'YA, 園芸学研究 別冊,   2007 03 24
  • カキ‘黒熊’由来のBC1集団における果実脱渋性の分離とRFLPマーカーによる完全甘ガキの選抜, KANZAKI SHIN'YA, KIMURA MIYUKI, YAMADA MASAHIKO, MITANI NOBUHITO, AKAGI TSUYOSHI, UTSUNOMIYA NAOKI, YONEMORI KEIZO, 園芸学研究 別冊,   2007 03 24
  • マンゴー‘Irwin’果実への低温処理が果実着色に及ぼす影響, YOSHIDA MARIKO, KANZAKI SHIN'YA, SHIMIZU KOSUKE, FUMURO MASAHIKO, SASAKI KATSUAKI, UTSUNOMIYA NAOKI, 熱帯農業,   2007
  • Possibility for identification of AST-locus in D. kaki by chromosome walking of D. lotus, AKAGI T, MASUKO T, KANZAKI S, MITANI N, YAMADA M, YONEMORI K, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2006 09 23
  • マイクロサテライトマーカーによる完全甘ガキ品種の識別, KANZAKI SHIN'YA, MAEDA YASUNORI, YAMADA MASAHIKO, MITANI NORIHITO, IWAMOTO KAZUHIKO, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2006 03 29
  • RFLPマーカーによるカキの甘渋性の識別, KANZAKI SHIN'YA, YONEMORI KEIZO, SUGIURA AKIRA, SATO AKIHIKO, YAMADA MASAHIKO, 果樹研究成果情報,   2006 02 01
  • 33. Effect of light and temperature on the fruit coloration of purple passionfruit, Utsunomiya N, Ide Y, Yamada K, Kanzaki S, Japanese journal of tropical agriculture,   2005 10 15
  • ムラサキクダモノトケイソウ果実の着色に及ぼす光と温度の影響, UTSUNOMIYA NAOKI, IDE YUKIKO, YAMADA TAKAKO, KAMIZAKI SHIN'YA, 熱帯農業,   2005 10 15
  • Effect of self and cross pollination on the fruit set and fruit growth in mango, UTSUNOMIYA N, NAKA S, KANZAKI S, SASAKI K, SHIMIZU K, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2005 10 01
  • AFLP法によるサンショウ(Zanthoxylum piperitum)の系統解析, KANZAKI SHIN'YA, MUTO KENTA, NAKAJIMA AKIHIRO, SAKO YOSHINAGA, SASAKI KATSUAKI, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2005 04 03
  • ウメ‘南高’の花芽の発達と葉芽の萌芽に及ぼす温度の影響, TAKAMATSU YOSHIHIRO, OTSUKA MAYO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2005 04 03
  • マンゴー‘アーウィン’果実における遮光処理がアントシアニン形成に及ぼす影響, UTSUNOMIYA NAOKI, OKAMOTO SATOSHI, NAKA SEN'ICHIRO, KANZAKI SHIN'YA, SASAKI KATSUAKI, SHIMIZU KOSUKE, 園芸学会雑誌 別冊,   2005 04 03
  • 36. Polymorphism analysis by AFLP method among seedlings from some passion fruit species, Kanzaki S, Asakura J, Utsunomiya N, Japanese journal of tropical agriculture,   2005 03 29
  • 35. Development of molecular makers for cultivar identification in lychee, Kanzaki S, Takamori S, Utsunomiya N, Japanese journal of tropical agriculture,   2005 03 29
  • Effect of temperature on the development of flower buds and budbreak of vegetative buds in Japanese apricot 'Nankho', TAKAMATSU Y, OTSUKA M, KANZAKI S, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2005 03 20
  • Effect of shade treatments on the anthocyanin occurrence in 'Arwin' mango fruit, UTSUNOMIYA N, OKAMOTO S, NAKA S, KANZAKI S, SASAKI K, SHIMIZU K, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2005 03 20
  • 45. Flower visiting insects and harmful insects on mango grown in plastichouse, Horikawa Y, Sakuratani Y, Kandori J, kanzaki S, Utsunomiya N, Simizu K, Sasaki K, Japanese journal of tropical agriculture,   2004 10 15
  • Effect of temperatures on flower bud development and flowering during different growth stages in 'Nanko' Japanese apricot, TAKAMATSU Y, KUBOTA I, CYUJYO A, MATSUI N, KANZAKI S, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2004 09 25
  • Effects of shading on the coloration of 'Irwin' mango fruit, Okamoto S, Takamatsu Y, Kanzaki S, Shimizu K, Sasaki . K, Utsunomiya N, Japanese journal of tropical agriculture,   2003 09 25
  • In vitroで得られたマンゴスチン幼植物体の生長に及ぼす諸要因の影響と接ぎ木の可能性, UEDA AKIO, TAKAMATSU YOSHIHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 熱帯農業,   2003 09 25
  • Bud dormancy and flower bud development as affected by high temperature in Japanese apricot 'Nanko', TAKAMATSU Y, YAMADA S, YAMAMOTO H, KANZAKI S, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2003 09 20
  • 果樹育種のためのDNAマーカー開発に向けた研究戦略と将来展望 3. 完全甘ガキ識別マーカーの開発とその育種への利用, KANZAKI SHIN'YA, 園芸学会雑誌 別冊,   2003 09 20
  • Verification of the marker-assisted selection system for PCNA phenotype of Japanese persimmon, KANZAKI S, SATO A, YAMADA M, MASUKO T, UTSUOMIYA N, YONEMORI K, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2003 04 04
  • Characterization of persimmon cultivars using SSR markers, WAKISAKA S, KANZAKI S, TAKAMATSU Y, UTSUNOMIYA N, J. Japan. Soc. Hort. Sci.,   2003 04 04
  • Effects of the inflorescence thinning on the yield and profit in ' Irwin' mango trees grown under plastic house condition, Shimizu K, Sasaki K, Takamatsu Y, Kanzaki S, Utsunomiya N, Japanese journal of tropical agriculture,   2003 03 27
  • Plant regeneration from internode tissue in passionfruit species, Ueki K, Takamatsu Y, Kanzaki S, Utsunomiya N, Japanese journal of tropical agriculture,   2003 03 27
  • ウメ‘南高’の休眠現象と花芽の発達に及ぼす低温の影響, TAKAMATSU YOSHIHIRO, ABE NOBUHISA, OYAMA HIROSHI, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2002 10 13
  • Characterization and segregation analysis of SSR markers in persimmon, WAKISAKA S, KANZAKI S, TAKAMATSU Y, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2002 10 13
  • Possibility of plant regeneration in vitro in Passiflore species, UEKI KANJI, TAKAMATSU YOSHIHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 熱帯農業,   2002 09 28
  • 46. Possibility of plant regeneration in vitro in Passiflora species, Ueki K, Takamatsu Y, Kanzaki S, Utsunomiya N, Japanese journal of tropical agriculture,   2002 09 28
  • 41. Effects of heading back pruning during winter season on panicle formation and fruit growth in the 'Irwin' mango trees grown under plastic house condition, Shimizu K, Sasaki K, Takamatsu Y, Kanzaki S, Utsunomiya N, Japanese journal of tropical agriculture,   2002 09 28
  • 40. Effect of pollen parents on fruit set and embryo development in mango, Sawada T, Takamatsu Y, Kanzaki K, Shimizu K, Sasaki K, Utsunomiya N, Japanese journal of tropical agriculture,   2002 09 28
  • ハウス栽培マンゴーにおける冬季のせん定処理が花穂形成と果実生長に及ぼす影響, SHIMIZU KOSUKE, SASAKI KATSUAKI, TAKAMATSU YOSHIHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 熱帯農業,   2002 09 28
  • 花粉親の違いがマンゴーの着果及びはい発育に及ぼす影響, SAWADA TOSHIHARU, TAKAMATSU YOSHIHIRO, KANZAKI SHIN'YA, SHIMIZU KOSUKE, SASAKI KATSUAKI, UTSUNOMIYA NAOKI, 熱帯農業,   2002 09 28
  • Effect of daylength on the development of leaf and flower buds in Japanese apricot, TAKAMATSU YOSHIHIRO, ABE NOBUHISA, OYAMA TAKUSHI, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2002 04 03
  • Isolation of SSR markers in persimmon, WAKISAKA S, KANZAKI S, TAKAMATSU Y, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2002 04 03
  • Non-astringent type persimmon in Luotian district of Hubei province in China, SATO A, KANZAKI S, YONEMORI K, IKEGAMI A, YAMADA M, LUO Z, WANG R, YANG Y, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2002 04 03
  • In vitroでのマンゴスチン幼植物体からの個体再生, UTSUNOMIYA NAOKI, CHINEN YOKO, KANZAKI SHIN'YA, TAKAMATSU YOSHIHIRO, 熱帯農業,   2002 03 27
  • 熱帯果樹における花器の形態的特色, UTSUNOMIYA NAOKI, KANZAKI SHIN'YA, INOUE HIROAKI, SUBHADRABANDHU S, SUGIURA AKIRA, 熱帯農業,   2001 10
  • AFLP法によるマンゴスチンの多様性解析の有効性, WAKISAKA SATOSHI, TAKAMATSU YOSHIHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, 熱帯農業,   2001 10
  • Possibility of inter specific hybrids with crossing in Passifloras, especially in purple passionfruit, UTSUNOMIYA NAOKI, KANZAKI SHIN'YA, TAKAMATSU YOSHIHIRO, 園芸学会雑誌 別冊,   2001 09 23
  • PCRを利用した完全甘ガキ個体の簡易判別法の可能性, NOSAKA MITSUNORI, KAMINZA SHIN'YA, YONEMORI KEIZO, SATO AKIHIKO, YAMADA MASAHIKO, SUGIURA AKIRA, 園芸学会雑誌 別冊,   2001 09 23
  • 走査型電子顕微鏡による4種の核果類の花芽および葉芽の観察, TAKAMATSU YOSHIHIRO, KUTSUKAKE YOKO, KANZAKI SHIN'YA, SASAKI KATSUAKI, TAKEBAYASHI TERUO, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2001 09 23
  • PCR method for selecting pollination-constant and non-astringent (PCNA) type persimmon, NOSAKA M, KANZAKI S, YONEMORI K, SATO A, YAMADA M, SUGIURA A, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2001 09 01
  • Scanning Electron Microscopy of flower and leaf bud development of four stone fruits species, TAKAMATSU Y, KUTSUKAKE Y, KANZAKI S, SASAKI K, TAKEBAYASHI T, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2001 09 01
  • マンゴー‘金こう’由来の実生の特性, KANZAKI SHIN'YA, KOKUDO KAZUTAKA, IMAMURA RUI, SASAKI KATSUAKI, UTSUNOMIYA NAOKI, 園芸学会雑誌 別冊,   2001 04 04
  • Analysis of the Genetic Relationships among Pollination-constant and Non-astringent (PCNA) Cultivars of Persimmon (Diospyros kaki Thunb.) from Japan and China using Amplified Fragment Length Polymorphism (AFLP), Kanzaki Shinya, Yonemori Keizo, Sato Akihiko, Yamada Masahiko, Sugiura Akira, Journal of the Japanese Society for Horticultural Science,   2000 11 15
    Summary:The genetic relationships among 19 pollination-constant and non-astringent (PCNA) cultivars and 14 non-PCNA cultivars of persimmon (Diospyros kaki Thunb.), including one Chinese PCNA cultivar 'Luo Tian Tian Shi', were analyzed by comparing 138 AFLP markers. A phylogenetic tree constructed based on the similarity indices of these AFLP markers indicated a close relationship among Japanese PCNA cultivars, but a more distant relationship with the Chinese PCNA cultivar 'Luo Tian Tian Shi'. The close relationship between PCNA cultivars native to Gifu prefecture was distinct, indicating that these cultivars developed from crosses among restricted sources in this region. The cultivars 'Gosho', 'Hana-gosho', 'Oo-gosho', and 'Yamato-gosho' showed a close relationship with some non-PCNA cultivars.
  • 「アーウィン」マンゴー樹における冬季の切り戻しせん定処理が開花・結実に及ぼす影響, SASAKI KATSUAKI, SHIMIZU KOSUKE, TAKAMATSU YOSHIHIRO, KANZAKI KEN'YA, UTSUNOMIYA NAOKI, 熱帯農業,   2000 10
  • Phylogenetic analysis of the genus Mangifera based on DNA sequences of ITS region, HONSHO C, KANZAKI S, YONEMORI K, EIADTHONG W, SUGIURA A, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 09 26
  • In vitro shoot formation from young fruit of fig, SENTOU A, KANZAKI S, TAKAMATSU Y, UTUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 09 26
  • Genetic relationships of persimmon (Diospyros kaki Thunb.) cultivars revealed by AFLP analysis, NOSAKA M, KANZAKI S, YONEMORI K, SATO A, YAMADA M, SUGIURA A, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 09 26
  • Inheritance of the trait for natural astringency-loss in the progeny of 'Luo Tian Tian Shi', IKEGAMI A, YONEMORI K, SATO A, YAMADA M, KANZAKI S, SUGIURA A, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 09 26
  • The effect of sludge compost as nitrogen fertilizer on the fruit growth and quality in potted grown strawberry, TAJIMA A, KANZAKI S, TAKAMATSU Y, UTSUNOMIYA N, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 09 26
  • Effectiveness of RFLP markers for selecting PCNA type in cultivars and progenies, KANZAKI S, YONEMORI K, SATO A, YAMADA M, SUGIURA A, 園芸学会雑誌. 別冊, 園芸学会大会研究発表,   2000 03 26
  • Preliminary analysis of phylogenetic relationships among Mangifera spp. in Thailand by a PCR-RFLP method using a special region of cpDNA., EIADTHONG W, KANZAKI SHIN'YA, YONEMORI KEIZO, SUGIURA AKIRA, UTSUNOMIYA NAOKI, NAKATSUBO FUMIAKI, SUBHADRABANDHU S, 園芸学会雑誌 別冊,   1997 03
  • Phylogenetic studies on Artocarpus spp. by RFLP of an amplified region of cpDNA., KANZAKI SHIN'YA, YONEMORI KEIZO, SUGIURA AKIRA, SUBHADRABANDHU S, 園芸学会雑誌 別冊,   1996 03

Misc

  • カキの甘渋性識別SCARマーカーを利用した完全甘ガキの選抜, 三谷宣仁, 河野淳, 山田昌彦, 神崎真哉, 佐藤明彦, 小林省藏, 伴雄介, 上野俊人, 白石美樹夫, 赤木剛士, 辻本誠幸, 米森敬三, 園芸学研究 別冊, 10, 1, 39,   2011 03 20 , http://jglobal.jst.go.jp/public/201102263324962698
  • 光質と窒素施肥量がハーブの香気ケモタイプに及ぼす影響, HAYASHI TAKAHIRO, NAKAMURA MINAKO, YOSHIOKA YUMI, NAKAGAWA MASAHIRO, KANZAKI SHIN'YA, UTSUNOMIYA NAOKI, SASAOKA MOTONOBU, ICHINOKI MUNEJI, YOSHIMI TAKANORI, SHIMADA TOMOHIRO, TOMI KEN'ICHI, MATSUMURA YASUKI, 園芸学研究 別冊, 9, 1, 212,   2010 03 21 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002271847594809
  • Phylogeny and cultivar development of Diospyros kaki: A survey based on molecular analyses, K. Yonemori, S. Kanzaki, C. Honsho, T. Akagi, D. E. Parfitt, Advances in Horticultural Science, 22, 261, 268,   2008 12 09 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149106746&origin=inward
    Summary:The relationships of Diospyros kaki to 14 Diospyros species, including D. glandulosa which was assumed as a progenitor of D. kaki by Ng (Malaysian Forester 41: 43-50), were studied by a combined data set of the sequences for ITS region and matK gene. Maximum parsimony, maximum likelihood, and neighbor joining analyses revealed that D. oleifera, a species native to China, was much closer to D. kaki than D. glandulosa. However, both D. oleifera and D. glandulosa were more closely related to D. kaki, compared with other species analyzed, indicating that these two diploid species might be involved in the origin of D. kaki, most of which is hexaploid. In addition, as for D. kaki, 102 Japanese, 20 Korean, and 24 Chinese cultivars were studied for their genetic relationships by comparing 496 AFLP markers to investigate cultivar development of D. kaki in Asia. An unrooted cladogram constructed by neighbor joining analysis indicated independent cultivar development in China, Korea, and Japan. Furthermore, pollination constant non-astringent (PCNA) type cultivars of Japanese origin were clearly separated from non-PCNA type cultivars in the cladogram, also indicating independent development of PCNA cultivars in Japan. Cultivar development of D. kaki in Asia is discussed further in the work, with focus on the astringency trait.
  • Flower visitors and pests of mango in the hothouse, 38, 19, 30,   2005 03
  • Production and conservation of Tomato yellow leaf curl virus-free parthenocarpic tomato plants using tissue culture, Sota Koeda, Rihito Takisawa, Tomoyuki Nabeshima, Yuri Tanaka, Akira Kitajima, Shotaro Matsumoto, Yuki Matsumoto, Shinya Kanzaki, 8th IGS & 6th ssDNA CVW,   2016 11
  • Development of EST-SSR Markers Derived from Diospyros kaki, T. Tsujimoto, T. Akagi, K. Yonemori, S. Kanzaki, V INTERNATIONAL SYMPOSIUM ON PERSIMMON, 996, 133, 137,   2013 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880441457&origin=inward
    Summary:For genetic analysis of persimmon (Diospyros kaki) and its relatives, we investigated the development of SSR markers with expression sequence tags (ESTs). Of 9475 EST data from the NCBI database which had isolated from D. kaki, 746 sequences contained SSRs which had more than four (tetranucleotide) or five (dinucleotide and trinucleotide) repeats. Tested on two species, D. kaki and D. lotus (a diploid relative of D. kaki), of primer pairs based on 65 SSR-containing EST sequences, 52 primer pairs resulted in proper PCR products. Some of these candidate EST-SSR markers showed polymorphism among cultivars and seedlings of D. kaki and D. lotus. Among tested seven clones of D. lotus, 32 of 52 candidate EST-SSR markers showed polymorphism. For molecular phylogenetic analysis, linkage mapping and whole genome mapping of Diospyros species, EST-SSR markers appear to be as useful as genomic SSR markers. In addition, development of EST-SSR markers seems to be easy and cost-effective when compared with genomic SSR markers.
  • Construction of a Reliable PCR Marker for Selecting Pollination Constant and Non-Astringent (PCNA) Type Offspring among Breeding Population of Persimmon (Diospyros kaki Thunb.), K. Yonemori, T. Akagi, S. Kanzaki, I INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF FRUIT SPECIES: BIOTECHFRUIT2008, 839, 625, 629,   2009 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=75649102794&origin=inward
    Summary:Pollination constant and non-astringent (PCNA) type persimmons, which lose their astringency naturally on the tree, are the most desirable for fresh fruit consumption. The trait of natural astringency-loss in PCNA type is recessive and controlled by a single locus, known as AST locus. Because PCNA type cultivars are of recent origin and exist only in a small number of cultivars, inbreeding depression resulting from repeated crosses among very few cultivars and/or selections is a major challenge facing the current breeding of new PCNA type cultivars. One solution to overcome this inbreeding depression is to include non-PCNA cultivars in the breeding project to extend the genetic pool by utilizing the wide diversity found in non-PCNA cultivars. However, to date, F-1 populations resulting from crosses between PCNA and non-PCNA type cultivars/selections have yielded only non-PCNA offspring. To obtain PCNA offspring, F-1 progeny needs to be backcrossed to PCNA type cultivars/selections. As persimmon is hexaploid, the obtainable rate of PCNA offspring in a backcross population is only 10-15%. Although we identified AFLP and RFLP markers tightly linked to the trait of natural astringency-loss in persimmon, these markers could not distinguish some non-PCNA progenies in other breeding populations. Previously, we demonstrated that RFLP markers derived from fosmid clones of D. lotus, diploid relatives of D. kaki, could be used to distinguish between PCNA and non-PCNA types of D. kaki, and established the possibility of extending this approach to PCR based markers. In this study, we describe the development of PCR-based markers derived from fosmid clones of D. kaki and D. lotus, and confirm their effectiveness across diverse progenies to select PCNA type.
  • Existence of several pollination constant non-astringent type persimmons in China, K Yonemori, A Ikegami, A Kitajima, A Sato, M Yamada, ZR Luo, Y Yang, RZ Wang, S Kanzaki, Proceedings of the Third International Symposium on Persimmon, 685, 685, 77, 83,   2005 , 10.17660/ActaHortic.2005.685.7, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149115285&origin=inward
    Summary:Pollination constant non-astringent (PCNA) type persimmons are unique in that they originate in Japan in recent era. The development of the PCNA type cultivars is not so advanced, since only six PCNA type cultivars were reported among more than 1,000 cultivars by a nationwide survey in Japan in 1912. The PCNA type cultivars are known to have very narrow genetic variability, and it causes a serious problem for breeding of new cultivars because of inbreeding depression in breeding population. On the other hand, in 1982, a new PCNA type cultivar, 'Luotian tianshi', was reported to be found in Luotian county in Hubei province. This PCNA type cultivar has a different genetic background from Japanese PCNA cultivars, since a cross between 'Luotian tianshi' and Japanese PCNA 'Taishu' yielded astringent type offspring in addition to PCNA type offspring. This means that the Chinese PCNA cultivar may provide a new strategy for persimmon breeding in the future. In addition to 'Luotian tianshi', it is believed that several non-astringent type persimmons exist in the same area of Luotian county in Hubei province where 'Luotian tianshi' was found. In this study, we have collected the fruits from several cultivars assumed to be a non-astringent type in Luotian county and determined whether or not those are true non-astringent cultivars of PCNA type by measuring tannin cell sizes of the fruits, which is a good criterion for distinguishing a PCNA type from a non-PCNA type. As a consequence, we could demonstrate that several PCNA type persimmons exist in Luotian county. These cultivars should be an important addition to persimmon germplasm and should have a potential role for breeding in the future.
  • Unique features of tannin cells in fruit of pollination constant non-astringent persimmons, K Yonemori, A Ikegami, S Kanzaki, A Sugiura, PROCEEDINGS OF THE 2ND INTERNATIONAL PERSIMMON SYMPOSIUM, 601, 601, 31, 35,   2003 , 10.17660/ActaHortic.2003.601.3, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=28844456013&origin=inward
    Summary:Among the four types of persimmon, the fruit of pollination constant nonastringent (PCNA) cultivars has a different development pattern of tannin cells in the flesh than the other three (PVNA, PNA, and PCA) types. The development of tannin cells in PCNA types seems to cease at early stages of fruit growth, so that tannin concentration in the flesh gradually decreases with fruit development by dilution. In order to clarify this difference between PCNA and non-PCNA types, the tannin cell size of 42 cultivars was investigated at fruit maturity in this study. Tannin cells were collected after EDTA maceration of the flesh, and the areas and both major and minor axes of 100 tannin cells were measured in each cultivar using computer imagery. Among the 42 cultivars examined, tannin cell sizes of all 15 PCNA cultivars were much smaller than all 27 non-PCNA cultivars. Even considering the density of tannin cells in the flesh, tannin cells of PCNA fruit occupied much smaller volumes (less than one-fifth on average) per weight of flesh than in non-PCNA cultivars. These results clearly show that the dilution of tannins is the main cause of natural astringency-loss in PCNA persimmons.
  • Molecular marker for selecting pollination-constant non-astringent (PCNA) type persimmon at the juvenile stage, K Yonemori, A Sugiura, A Sato, M Yamada, S Kanzaki, GENETICS AND BREEDING OF TREE FRUITS AND NUTS, 622, 622, 189, 203,   2003 , 10.17660/ActaHortic.2003.622.16, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052772185&origin=inward
    Summary:Persimmon (Diospyros kaki Thunb.) is classified into four types depending on the nature of astringency-loss and the change in the flesh color. Among them, the pollination constant and non-astringent (PCNA) type is the most desirable for fruit consumption. Since natural astringency loss in PCNA type persimmon is qualitatively inherited and recessive to the non-PCNA trait, an investigation to find dominant markers for the trait was carried out for efficient selection of PCNA type plants in breeding populations. Amplified fragment length polymorphism (AFLP) technique was applied for this purpose using bulked segregant analysis (BSA). A total of 128 primer combinations were tested and one AFLP marker was found to be linked to the dominant allele controlling the trait for astringency. This marker, EACC/MCTA-400, was absent in all of the PCNA-type plants tested, but present in about half of the non-PCNA type plants tested. Furthermore, RFLP analysis using this marker as a probe enabled the detection of the other dominant allele, and all PCNA-type plants could be distinguished from non-PCNA type plants. The effectiveness of this RFLP markers was confirmed by the same analysis to different breeding progenies and to existing cultivars. Currently, we are trying to establish an effective system for the selection of PCNA plants from breeding populations by applying this RFLP analysis.
  • Chemotaxonomic studies on some Diospyros species by flavonoid compositions in leaves., S Kanzaki, A Sugiura, K Yonemori, N Utsunomiya, S Subhadrabandhu, FIRST INTERNATIONAL PERSIMMON SYMPOSIUM, 436, 436, 93, 99,   1997 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=13744260059&origin=inward
    Summary:Flavonoid compositions in the leaves of 24 Diospyros species were surveyed by HPLC for chemotaxonomic purpose. The species analyzed include D.areolata, D.castanea, D.buxifolia D.confertiflora, D.decandra, D.discolor , D.ehretioides, D. D.glandulosa, D.gracilis, D.malabarica var. siamensis, D.mollis, D.montana, D. pyrrhocarpa, D.rhodocalyx, D.sandwicensis, D.sumatrana, D.toposia, D.variegata, and D.wallichii, all for tropical and subtropical origin, and D.lotus, D.oleifera, D.kaki, D. rhombifolia and D.virginiana, for temperate origin. Among these species, D.areolata, D. castanea, D.confertiflora, L.gracilis, D.malabarica var. siamensis, D.pyrrhocarpa, D. toposia and D.variegata showed no peak for flavonoids by HPLC, while the others had 1 to. 10 peaks. Species native to temperate regions had more peaks than those native to tropical and subtropical regions. When cluster analysis by UPGM was done based on dissimilarities of these peaks, temperate species showed close relationship to each other, except for D.rhombifolia. D.glandulosa, which is supposed to be closely related to D. kaki by its similarity in morphology, could not show a close relationship to D.kaki in the present study.

Awards & Honors

  •   2011 03 , Excellent paper, SCAR markers for practical application of marker-assisted selection in persimmon (Diospyros kaki) breeding.

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Characterization of intervarietal crossing and establishment of base for breeding in mango., To develop an practical method for determining the male parent of mango seedlings, high-polymorphic SSR markers were selected and multiplex PCR method was developed. Progenies obtained from open pollinated 'Irwin' trees and 'Beni-Keitt' trees in a plastic house were used for determining male parents. The results showed that cross-pollinated seedlings were major in both cultivars. In addition, Myb transcription factor associated with anthocyanin biosynthesis were isolated from mango and its structure was analyzed.
  • Cooperative Research, Breeding and Genetics for Some Tropical Fruits
  • Cooperative Research, Survey of specific markers for PCNA nature of Japanese Persimmon