KINDAI UNIVERSITY


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KISHIDA Kunihiro

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FacultyDepartment of Science and Technology on Food Safety / Graduate School of Biology-Oriented Science and Technology
PositionAssociate Professor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/847-kishida-kunihiro.html
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Last Updated :2020/09/02

Education and Career

Education

  •   1996 04  - 2000 03 , Osaka City University, Faculty of Human Life Science
  •   2000 04  - 2002 03 , Osaka City University, Graduate School of Human Life Science
  •   2002 04  - 2005 03 , Osaka City University, Graduate School of Human Life Science

Academic & Professional Experience

  •   2017 04 ,  - 現在, Faculty of Biology-Oriented Science and Technology, Department of Science and Technology on Food Safety, Kindai University
  •   2010 04 ,  - 2017 03 , Faculty of Biology-Oriented Science and Technology, Department of Science and Technology on Food Safety, Kindai University
  •   2015 08 ,  - 2016 08 , Visiting Researcher, Rutgers University, New Jersey Medical School
  •   2008 04 ,  - 2010 03 , University of Nagasaki
  •   2007 04 ,  - 2008 03 , Faculty of Nursing and Nutrition, Department of Nutrition, Siebold University of Nagasaki
  •   2006 04 ,  - 2007 03 , Faculty of Nursing and Nutrition, Department of Nutrition, Siebold University of Nagasaki
  •   2005 04 ,  - 2006 03 , Kyushu Nutrition Welfare University, Kyushu Nutrition Welfare University, Kyushu Nutrition Welfare University

Research Activities

Research Areas

  • Life sciences, Physiology
  • Humanities & social sciences, Home economics, lifestyle science

Published Papers

  • Effects of Medium Chain Triglycerides Intake on Lipid Metabolism and Intestinal Disaccharidase Activities in Rats, Erika Suzuki, Moe Oshima, Masayuki Sonotsuka, Hiroyo Hayashitani, Seiji Ohtani, Kunihiro Kishida, YAKUGAKU ZASSHI, YAKUGAKU ZASSHI, 140(8), 1051 - 1061, Aug. 01 2020 , Refereed
  • Cell-Type–Specific, Ketohexokinase-Dependent Induction by Fructose of Lipogenic Gene Expression in Mouse Small Intestine, Arwa Al-Jawadi, Chirag R Patel, Reilly J Shiarella, Emmanuellie Romelus, Madelyn Auvinen, Joshua Guardia, Sarah C Pearce, Kunihiro Kishida, Shiyan Yu, Nan Gao, Ronaldo P Ferraris, The Journal of Nutrition, The Journal of Nutrition, 150(7), 1722 - 1730, Jul. 01 2020 , Refereed
    Summary:ABSTRACT Background High intakes of fructose are associated with metabolic diseases, including hypertriglyceridemia and intestinal tumor growth. Although small intestinal epithelia consist of many different cell types, express lipogenic genes, and convert dietary fructose to fatty acids, there is no information on the identity of the cell type(s) mediating this conversion and on the effects of fructose on lipogenic gene expression. Objectives We hypothesized that fructose regulates the intestinal expression of genes involved in lipid and apolipoprotein synthesis, that regulation depends on the fructose transporter solute carrier family 2 member a5 [Slc2a5 (glucose transporter 5)] and on ketohexokinase (Khk), and that regulation occurs only in enterocytes. Methods We compared lipogenic gene expression among different organs from wild-type adult male C57BL mice consuming a standard vivarium nonpurified diet. We then gavaged twice daily for 2.5 d fructose or glucose solutions (15%, 0.3 mL per mouse) into wild-type, Slc2a5-knockout (KO), and Khk-KO mice with free access to the nonpurified diet and determined expression of representative lipogenic genes. Finally, from mice fed the nonpurified diet, we made organoids highly enriched in enterocyte, goblet, Paneth, or stem cells and then incubated them overnight in 10 mM fructose or glucose. Results Most lipogenic genes were significantly expressed in the intestine relative to the kidney, liver, lung, and skeletal muscle. In vivo expression of Srebf1, Acaca, Fasn, Scd1, Dgat1, Gk, Apoa4, and Apob mRNA and of Scd1 protein increased (P < 0.05) by 3- to 20-fold in wild-type, but not in Slc2a5-KO and Khk-KO, mice gavaged with fructose. In vitro, Slc2a5- and Khk-dependent, fructose-induced increases, which ranged from 1.5- to 4-fold (P < 0.05), in mRNA concentrations of all these genes were observed only in organoids enriched in enterocytes. Conclusions Fructose specifically stimulates expression of mouse small intestinal genes for lipid and apolipoprotein synthesis. Secretory and stem cells seem incapable of transport- and metabolism-dependent lipogenesis, occurring only in absorptive enterocytes.
  • Urinary excretion rate and bioavailability of chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid in non-fasted rats maintained under physiological conditions, Kunihiro Kishida, Harumi Matsumoto, Heliyon, Heliyon, 5(10), e02708, Oct. 2019 , Refereed
  • d-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine., Kishida K, Martinez G, Iida T, Yamada T, Ferraris RP, Toyoda Y, Food chemistry, Food chemistry, 277, 604 - 608, Mar. 2019 , Refereed
  • Antimicrobial Activity of the Phenolic Compounds of Prunus mume against Enterobacteria., Mitani T, Ota K, Inaba N, Kishida K, Koyama HA, Biological & pharmaceutical bulletin, Biological & pharmaceutical bulletin, 41(2), 208 - 212, 2018 , Refereed
  • Characterization of Phenolics in the Flesh Tissue of ’Tsuyuakane' a Red Flesh Type of Japanese Apricot (Prunus mume Sieb. et Zucc.) and its Effect on Postprandial Blood Glucose Levels in Rats, HORINISHI Asako, YUKIMOTO Ryo, HIGASHIDE Kohei, KISHIDA Kunihiro, MORI Hajime, OZAKI Yoshihiko, Food Preservation Science, Food Preservation Science, 43(4), 153 - 162, Jul. 2017 , Refereed
  • 果実由来の機能性成分によるメタボリックシンドローム予防および改善に関する研究, 岸田邦博, 日本食品保蔵科学会誌, 日本食品保蔵科学会誌, 42(2), 79‐85, Mar. 31 2016
  • Effect of Citrus Limonoids on Hepatic Gene Expression Involved in Lipid Metabolism in Rats, KISHIDA KUNIHIRO, SUZUKI MASAYA, IHARA HAYATO, OZAKI YOSHIHIKO, 日本食品保蔵科学会誌, 日本食品保蔵科学会誌, 41(2), 51 - 58, Mar. 2015 , Refereed
  • Phenolic Extract from Japanese Apricot Fruit (Prunus Mume Sieb et Zucc.) Inhibits Disaccharidase Activity in the Small Intestine and Suppresses the Postprandial Elevation of Glucose Levels in Rats, KISHIDA KUNIHIRO, SUZUKI MASAYA, HEYA YUICHI, YAMAZAKI YUDAI, HORINISHI ASAKO, OZAKI YOSHIHIKO, Food preservation science, Food preservation science, 40(3), 119 - 125, May 2014 , Refereed
  • Bone Growth is Influenced by Fructose in Adolescent Male Mice Lacking Ketohexokinase (KHK)., Edek A J Williams, Veronique Douard, Keiichiro Sugimoto, Hiroshi Inui, Fabienne Devime, Xufei Zhang, Kunihiro Kishida, Ronaldo P Ferraris, J Christopher Fritton, Calcified tissue international, Calcified tissue international, 106(5), 541 - 552, May 2020 , Refereed
    Summary:Fructose is metabolized in the cytoplasm by the enzyme ketohexokinase (KHK), and excessive consumption may affect bone health. Previous work in calcium-restricted, growing mice demonstrated that fructose disrupted intestinal calcium transport. Thus, we hypothesized that the observed effects on bone were dependent on fructose metabolism and took advantage of a KHK knockout (KO) model to assess direct effects of high plasma fructose on the long bones of growing mice. Four groups (n = 12) of 4-week-old, male, C57Bl/6 background, congenic mice with intact KHK (wild-type, WT) or global knockout of both isoforms of KHK-A/C (KHK-KO), were fed 20% glucose (control diet) or fructose for 8 weeks. Dietary fructose increased by 40-fold plasma fructose in KHK-KO compared to the other three groups (p < 0.05). Obesity (no differences in epididymal fat or body weight) or altered insulin was not observed in either genotype. The femurs of KHK-KO mice with the highest levels of plasma fructose were shorter (2%). Surprisingly, despite the long-term blockade of KHK, fructose feeding resulted in greater bone mineral density, percent volume, and number of trabeculae as measured by µCT in the distal femur of KHK-KO. Moreover, higher plasma fructose concentrations correlated with greater trabecular bone volume, greater work-to-fracture in three-point bending of the femur mid-shaft, and greater plasma sclerostin. Since the metabolism of fructose is severely inhibited in the KHK-KO condition, our data suggest mechanism(s) that alter bone growth may be related to the plasma concentration of fructose.
  • Marked differences in tight junction composition and macromolecular permeability among different intestinal cell types, Sarah C. Pearce, Arwa Al-Jawadi, Kunihiro Kishida, Shiyan Yu, Madeleine Hu, Luke F. Fritzky, Karen L. Edelblum, Nan Gao, Ronaldo P. Ferraris, BMC Biology, BMC Biology, 16(1), 19, Feb. 01 2018 , Refereed
    Summary:Background: Mammalian small intestinal tight junctions (TJ) link epithelial cells to one another and function as a permselective barrier, strictly modulating the passage of ions and macromolecules through the pore and leak pathways, respectively, thereby preventing the absorption of harmful compounds and microbes while allowing regulated transport of nutrients and electrolytes. Small intestinal epithelial permeability is ascribed primarily to the properties of TJs between adjoining enterocytes (ENTs), because there is almost no information on TJ composition and the paracellular permeability of nonenterocyte cell types that constitute a small but significant fraction of the intestinal epithelia. Results: Here we directed murine intestinal crypts to form specialized organoids highly enriched in intestinal stem cells (ISCs), absorptive ENTs, secretory goblet cells, or Paneth cells. The morphological and morphometric characteristics of these cells in organoids were similar to those in vivo. The expression of certain TJ proteins varied with cell type: occludin and tricellulin levels were high in both ISCs and Paneth cells, while claudin-1, -2, and -7 expression was greatest in Paneth cells, ISCs, and ENTs, respectively. In contrast, the distribution of claudin-15, zonula occludens 1 (ZO-1), and E-cadherin was relatively homogeneous. E-cadherin and claudin-7 marked mainly the basolateral membrane, while claudin-2, ZO-1, and occludin resided in the apical membrane. Remarkably, organoids enriched in ENTs or goblet cells were over threefold more permeable to 4 and 10 kDa dextran compared to those containing stem and Paneth cells. The TJ-regulator larazotide prevented the approximately tenfold increases in dextran flux induced by the TJ-disrupter AT1002 into organoids of different cell types, indicating that this ZO toxin nonselectively increases permeability. Forced dedifferentiation of mature ENTs results in the reacquisition of ISC-like characteristics in TJ composition and dextran permeability, suggesting that the post-differentiation properties of TJs are not hardwired. Conclusions: Differentiation of adult intestinal stem cells into mature secretory and absorptive cell types causes marked, but potentially reversible, changes in TJ composition, resulting in enhanced macromolecular permeability of the TJ leak pathway between ENTs and between goblet cells. This work advances our understanding of how cell differentiation affects the paracellular pathway of epithelia.
  • Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells, Kunihiro Kishida, Sarah C. Pearce, Shiyan Yu, Nan Gao, Ronaldo P. Ferraris, AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 312(6), G592 - G605, Jun. 2017 , Refereed
    Summary:Nutrient sensing triggers responses by the gut-brain axis modulating hormone release, feeding behavior and metabolism that become dysregulated in metabolic syndrome and some cancers. Except for absorptive enterocytes and secretory enteroendocrine cells, the ability of many intestinal cell types to sense nutrients is still unknown; hence we hypothesized that progenitor stem cells (intestinal stem cells, ISC) possess nutrient sensing ability inherited by progenies during differentiation. We directed via modulators of Wnt and Notch signaling differentiation of precursor mouse intestinal crypts into specialized organoids each containing ISC, enterocyte, goblet, or Paneth cells at relative proportions much higher than in situ as determined by mRNA expression and immunocytochemistry of cell type biomarkers. We identified nutrient sensing cell type(s) by increased expression of fructolytic genes in response to a fructose challenge. Organoids comprised primarily of enterocytes, Paneth, or goblet, but not ISC, cells responded specifically to fructose without affecting nonfructolytic genes. Sensing was independent of Wnt and Notch modulators and of glucose concentrations in the medium but required fructose absorption and metabolism. More mature enterocyte- and gobletenriched organoids exhibited stronger fructose responses. Remarkably, enterocyte organoids, upon forced dedifferentiation to reacquire ISC characteristics, exhibited a markedly extended lifespan and retained fructose sensing ability, mimicking responses of some dedifferentiated cancer cells. Using an innovative approach, we discovered that nutrient sensing is likely repressed in progenitor ISCs then irreversibly derepressed during specification into sensing-competent absorptive or secretory lineages, the surprising capacity of Paneth and goblet cells to detect fructose, and the important role of differentiation in modulating nutrient sensing. NEW & NOTEWORTHY Small intestinal stem cells differentiate into several cell types transiently populating the villi. We used specialized organoid cultures each comprised of a single cell type to demonstrate that 1) differentiation seems required for nutrient sensing, 2) secretory goblet and Paneth cells along with enterocytes sense fructose, suggesting that sensing is acquired after differentiation is triggered but before divergence between absorptive and secretory lineages, and 3) forcibly dedifferentiated enterocytes exhibit fructose sensing and lifespan extension.
  • Safety and adherence of Umezu polyphenols in the Japanese plum (Prunus mume) in a 12-week double-blind randomized placebo-controlled pilot trial to evaluate antihypertensive effects, Shigeki Takemura, Kouichi Yoshimasu, Jin Fukumoto, Kanae Mure, Nobuhiro Nishio, Kunihiro Kishida, Fumiko Yano, Takahiko Mitani, Tatsuya Takeshita, Kazuhisa Miyashita, ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE, ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE, 19(6), 444 - 451, Nov. 2014 , Refereed
    Summary:Medications or lifestyle changes to prevent or improve hypertension often press considerable efforts on patients suffering from mild hypertension. Capsules including Umezu polyphenols (UP), polyphenols in Japanese plums, may help them to control their blood pressure (BP). The aim of this study is to evaluate the effectiveness of UP on BP and its safety. A total of 15 healthy workers without antihypertensive medication who had some concerns about their BP, preferably normal-high BP or hypertension level 1, were randomized in a double-blind manner into UP ingesting and placebo groups. Each subject was instructed to take four capsules daily for 12 weeks (daily UP dose, 800 mg for the UP ingesting group; and 0 mg for the placebo group). These subjects were followed for 12 weeks, and their BP both at home and at the examination site, as well as self-perceived quality-of-life outcomes and possible side effects, was monitored during that period. Group x time interactions on BP changes were examined. All of the 15 subjects completed the 12-week intervention trial. The BP changes did not significantly differ between the UP ingesting and placebo groups, neither at the examination site nor at home. But during the study period, no adverse effects were observed. No remarkable effect of UP on BP was observed. However, a higher dose of UP was confirmed safe and high in adherence in this 12-week randomized controlled trial. Its effect on BP and other outcomes shall be confirmed in a larger sample.
  • Phenolics profile of mume, Japanese apricot (Prunus mume Sieb. et Zucc.) fruit, Takahiko Mitani, Asako Horinishi, Kunihiro Kishida, Tomoaki Kawabata, Fumiko Yano, Hisa Mimura, Nobuya Inaba, Hisako Yamanishi, Takaaki Oe, Keiichi Negoro, Hajime Mori, Yasuhito Miyake, Asao Hosoda, Yoshie Tanaka, Megumi Mori, Yoshihiko Ozaki, Bioscience, Biotechnology and Biochemistry, Bioscience, Biotechnology and Biochemistry, 77(8), 1623 - 1627, 2013 , Refereed
    Summary:The fruit of mume, Japanese apricot (Prunus mume Sieb. et Zucc.), was evaluated for its phenolics content, high performance liquid chromatography (HPLC) pro- file and antioxidative activities. The phenolics content of mume fruit was relatively high, the flesh of fully matured fruit containing up to 1% of phenolics on a dry weight basis. Reflecting such a high content of phenolics, the ORAC (oxygen radical absorbance capacity) value for mume fruit flesh showed high values, ranging from 150 to 320 μmol/g Trolox equivalent, depending upon the stage of maturation. 5-O-Caffeoylqunic acid (chlorogenic acid), 3-O-caffeoylquinic acid and tetra-O-acylated sucrose-related compounds were isolated from the flesh of mume fruit, although many unknown peaks were also apparent in the HPLC chromatogram. An alkali hydrolysate comprised four main phenolic acids, caffeic acid, cis/trans-p-coumaric acid and ferulic acid. No flavonoids were observed in the analysis. These results suggest that the majority of phenolics in mume fruit were hydroxycinnamic acid derivatives.
  • Simplified extraction of tetracycline antibiotics from milk using a centrifugal ultrafiltration device, Kunihiro Kishida, FOOD CHEMISTRY, FOOD CHEMISTRY, 126(2), 687 - 690, May 2011 , Refereed
    Summary:A simplified method was developed for the simultaneous determination of oxytetracycline, tetracycline, chlortetracycline, and doxycycline in milk. Isolation of the target compounds was performed using an Ultrafree-MC/PL centrifugal ultrafiltration device without prior sample preparation. Analyses were carried out via high-performance liquid chromatography using a Discovery HS F5 column with a gradient mobile phase which consisted of 30 mM citric acid solution (pH 3, in water) and acetonitrile (60:40 -> 40:60, v/v). Recovery of the target compounds from spiked samples at four levels (0.05, 0.1, 0.2, and 0.5 mu g/mL) was higher than 87%, with relative standard deviations of less than 6%. Limits of quantification ranged from 0.01 to 0.04 mu g/mL. (C) 2010 Elsevier Ltd. All rights reserved.
  • An Association Analysis between Mitochondrial DNA A10398G Polymorphism and Temperament in Japanese Young Adults, Kunihiro Kishida, Mihoko Tominaga, Kiminori Matsubara, Masanori Taguchi, Masanori Noguchi, Noriaki Tsunawake, Yoshihiro Shidoji, PLOS ONE, PLOS ONE, 4(11), e7763, Nov. 2009 , Refereed
    Summary:The mitochondrial (mt) DNA C5178A and A10398G polymorphisms have been reported to be associated with mental disorders such as bipolar disorder. However, the effects of these polymorphisms on temperament in healthy people are poorly understood. Evaluating healthy subjects can have the advantage of providing new strategies for maintaining psychological health and preventing mental illness. We examined the association between mtDNA polymorphisms and temperament in Japanese students. There was no significant difference in examined temperament when analysed by genotypes, 5178-10398 haplotypes, or sex. The subgroup analysis based on sex indicated that there was an interactive effect of the mtDNA A10398G polymorphism and sex on anxiety and obsession. This finding is preliminary and cannot exclude the possibility of false-positive due to small sample size (144 subjects) and multiple statistical testing. Further studies involving a larger sample size or other ethnic groups are necessary to confirm that mtDNA A10398G polymorphism can be a genetic factor for temperament.
  • The Ala54Thr polymorphism in the fatty acid-binding protein 2 gene leads to higher food intake in Japanese women, K. Kishida, M. Aoyama, M. Masaki, Y. Shidoji, MOLECULAR PSYCHIATRY, MOLECULAR PSYCHIATRY, 14(5), 466 - 467, May 2009 , Refereed
  • Quantitation and confirmation of six sulphonamides in meat by liquid chromatography-mass spectrometry with photodiode array detection, Kunihiro Kishida, FOOD CONTROL, FOOD CONTROL, 18(4), 301 - 305, May 2007 , Refereed
    Summary:Multiresidue analysis of six sulphonamides (SAs) (sulphadiazine, sulphadimidine, sulphamonomethoxine, sulphamethoxazole, sulphadimethoxine, and sulphaquinoxaline) in meat (beef, pork, and chicken) using liquid chromatography (LC)-mass spectrometry (MS) with photodiode array (PDA) detection is presented. The sample preparation is carried out by normal-phase matrix solid-phase dispersion (MSPD) with an ethanol solution. The LC-MS determination is performed using a Mightysil RP-4 GP column and an isocratic mobile phase of 0.3% (v/v) acetic acid solution (pH 3.4, in water)-ethanol (83:17, v/v) with an atmospheric pressure chemical ionization (APCI) MS on positive-ion mode. Average recoveries spiked at 0.05-0.5 ppm for each drug were higher than 90% with relative standard deviations between 1% and 6%. In all the processes, no toxic solvents were used at all. (C) 2005 Elsevier Ltd. All rights reserved.
  • Restricted-access media liquid chromatography for determination of sulfamonomethoxine, sulfadimethoxine, and their N-4-acetyl metabolites in eggs, Kunihiro Kishida, FOOD CHEMISTRY, FOOD CHEMISTRY, 101(1), 281 - 285, 2007 , Refereed
    Summary:Simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their N-4-acetyl metabolites in eggs using restricted-access media high performance liquid chromatography (RAM-HPLC) with a photo-diode array detector is developed. The target compounds are extracted by a handheld ultrasonic homogenizer with saturated ammonium sulfate solution followed by centrifugation. The separation is performed by a Hisep shielded hydrophobic phase column, isocratic elution with 0.3% acetic acid solution (pH 2.9, in water)-ethanol (75:25, v/v). Average recoveries from samples spiked at 0.1-1.0 ppm for each drug were > 91% with relative standard deviations within 4%. The limits of quantitation were <= 0.08 ppm. No hazardous-chemicals were used in all the processes. (c) 2006 Elsevier Ltd. All rights reserved.
  • Determining Zeranol in bovine tissues under nontoxic conditions, N Furusawa, K Kishida, LC GC NORTH AMERICA, LC GC NORTH AMERICA, 82 - 85, Feb. 2006 , Refereed
    Summary:The authors have developed a method of determining zeranol residues in bovine tissues (muscle, kidney, liver, and fat) without using toxic chemicals, organic solvents, and reagents in sample preparation and reversed-phase high performance liquid chromatography (HPLC) separation. Isolation is achieved by homogenization using a handheld ultrasonic homogenizer, which is easy to use and portable, followed by solid-phase extraction with an anion exchanger. For determination and identification of zeranol, the HPLC method uses an isocratic mobile phase and a photodiode-array detector. The authors obtained average recoveries of zeranol > 84% with relative standard deviations around 3.0%-4.8% in spiked samples. The total analytical time and quantitation limit were < 40 min/sample and 0.04 mu g/g, respectively.
  • Determining zeranol in bovine tissues under nontoxic conditions, N Furusawa, K Kishida, LC GC NORTH AMERICA, LC GC NORTH AMERICA, 24(1), 58 - +, Jan. 2006
    Summary:The authors have developed a method of determining zeranol residues in bovine tissues (muscle, kidney, liver, and fat) without using toxic chemicals, organic solvents, and reagents in sample preparation and reversed-phase high performance liquid chromatography (HPLC) separation. Isolation is achieved by homogenization using a handheld ultrasonic homogenizer, which is easy to use and portable, followed by solid-phase extraction with an anion exchanger. For determination and identification of zeranol, the HPLC method uses an isocratic mobile phase and a photodiode-array detector. The authors obtained average recoveries of zeranol > 84% with relative standard deviations around 3.0%-4.8% in spiked samples. The total analytical time and quantitation limit were < 40 min/sample and 0.04 mu g/g, respectively.
  • A, green, low-cost method for determining sulphonamides in milk, N Furusawa, K Kishida, LC GC EUROPE, LC GC EUROPE, 18(11), 600 - +, Nov. 2005
    Summary:Presented is a sample preparation-high performance liquid chromatography-diode array detection method for the simultaneous determination of five sulphonamides in milk. The procedure, performed under 100% aqueous conditions, does not use organic solvents or reagents and is, therefore, harmless to both humans and the environment.
  • Simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their hydroxy/N-4-acetyl metabolites with gradient liquid chromatography in chicken plasma, tissues, and eggs, K Kishida, N Furusawa, TALANTA, TALANTA, 67(1), 54 - 58, Jul. 2005 , Refereed
    Summary:A simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their hydroxy/N-4-acetyl metabolites in chicken plasma, muscle, liver, and eggs using gradient high-performance liquid chromatography (HPLC) with a photo-diode array detector is developed. All the compounds are extracted by a handheld ultrasonic homogenizer with ethanol followed by centrifugation. The separation is performed by a reversed-phase C4 column with a gradient elution (ethanol: 1% (v/v) acetic acid, v/v; 10: 90 --> 20:80). Average recoveries from samples spiked at 0.1-1.0 mu g g(-1) or mu g ml(-1) for each drug were >90% with relative standard deviations within 4%. The limits of quantitation were <30 ng g(-1) or ng ml(-1). (C) 2005 Elsevier B.V. All rights reserved.
  • Liquid chromatographic determination of sulfamonomethoxine, sulfadimethoxine, and their N-4-acetyl metabolites in chicken plasma, K Kishida, K Nishinari, N Furusawa, CHROMATOGRAPHIA, CHROMATOGRAPHIA, 61(1-2), 81 - 84, Jan. 2005
    Summary:An "environmentally-friendly" method has been established for simultaneous determination of sulfamonomethoxine, sulfadimethoxine, and their N-4-acetyl metabolites in chicken plasma. The sample is prepared by mixing with 4 mol L-1 ammonium sulfate solution then centrifugation, and analysis is performed by high-performance liquid chromatography (HPLC) on a polyethylene glycol reversed-phase column with 0.001 mol L-1 sodium acetate solution as mobile phase and photodiode-array detection. Average recoveries from samples spiked with 0.1, 0.5, and 1.0 mug mL(-1) of each drug were >78% and relative standard deviations were within 4%. The practical quantitation limits were less than or equal to0.09 mug mL(-1). No organic solvents or hazardous reagents were used at any stage of the analysis.
  • Determining sulfamethazine in pork using HPLC with a 100% water mobile phase, N Furusawa, K Kishida, LC GC NORTH AMERICA, LC GC NORTH AMERICA, 22(11), 1092 - +, Nov. 2004
    Summary:The authors have developed a method for determining sulfamethazine residue in pork using 100% water in sample preparation and high performance liquid chromatography (HPLC) separation. Sample preparation comprises homogenization with 100% water by means of a handheld ultrasonic homogenizer followed by centrifugal ultrafiltration. HPLC is performed on a reversed-phase C1 column with a 100% water mobile phase and a photodiode-array detector. The average recoveries, total analytical time, and limit of quantitation were less than or equal to 81 % (relative standard deviations were < 5.8%), < 30 min/sample, and 0.09 mug/g, respectively.
  • Application of shielded column liquid chromatography for determination of sulfamonomethoxine, sulfadimethoxine, and their N-4-acetyl metabolites in milk, K Kishida, N Furusawa, JOURNAL OF CHROMATOGRAPHY A, JOURNAL OF CHROMATOGRAPHY A, 1028(1), 175 - 177, Feb. 2004 , Refereed
    Summary:A hazardous-chemical free method for simultaneous determination of sulfamonomethoxine (SMM), sulfadimethoxine (SDM), and their N-4-acetyl metabolites in raw milk using shielded column liquid chromatography is developed. The target analytes are extracted by mixing with ethanol-acetic acid (97:3, v/v) followed by centrifugation. The procedure uses a Hisep shielded hydrophobic phase (SUP) column, isocratic elution with 0.1% acetic acid solution (pH 3.1, in water)-ethanol (75:25, v/v), and a photo-diode array detector. Average recoveries from samples spiked at 25-500 ng/ml for each drug were >81% with relative standard deviations within 5%. The limits of quantitation were <25 ng/ml. (C) 2004 Elsevier B.V. All rights reserved.
  • Toxic/harmful solvents-free technique for HPLC determination of six sulfonamides in meat, K Kishida, N Furusawa, JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 26(17), 2931 - 2939, 2003
    Summary:A simultaneous HPLC determination of six sulfonamides (SAs) [sulfadiazine (SDA), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfadimethoxine (SDM), and sulfaquinoxaline (SQ)] in meat (chicken, beef, and pork) is presented. The sample preparation is carried out by normal-phase matrix solid-phase dispersion (MPSD) with an aqueous ethanol solution. The HPLC determination is performed using a Mightysil RP-4 GP column and an isocratic mobile phase of 2% (v/v) acetic acid solution (pH 2.7, in water)-ethanol (75: 25, v/v) with a photodiode array detector. Average recoveries spiked at 0.05-0.5 ppm for each drug are higher than 85% with standard deviations within 10%. In all the processes, no toxic/harmful solvents are used at all.
  • Transfer and distribution profiles of dietary sulphonamides in the tissues of the laying hen, N Furusawa, K Kishida, FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 19(4), 368 - 372, Apr. 2002 , Refereed
    Summary:The five sulphonamides, sulphadiazine (SDZ), sulphadimidine (SDD), sulphamethoxazole (SMX), sulphamonomethoxine (SMM) and sulphaquinoxaline (SQ), were fed to laying hens at a dietary concentration of 100 mg kg(-1), respectively. On the 7th day after the start of feeding, the drug concentrations in the plasma, muscle and the main tissues involved in egg formation, the liver, and ovary and oviducts (magnum and isthmus plus shell grand) were determined by high-performance liquid chromatography. Dietary sulphonamides were distributed throughout the above tissues. SQ was found at the highest concentration in all tissues, while the reverse was true for SDD. The ratio of SDD concentrations in the main tissues involved in egg formation to that in the plasma were greater than those for the other drugs.
  • Transfer and distribution profiles of dietary sulphonamides in the tissues of the laying hen, N. Furusawa, K. Kishida, Food Additives and Contaminants, Food Additives and Contaminants, 19(4), 368 - 372, 2002
    Summary:The five sulphonamides, sulphadiazine (SDZ), sulphadimidine (SDD), sulphamethoxazole (SMX), sulphamonomethoxine (SMM) and sulphaquinoxaline (SQ), were fed to laying hens at a dietary concentration of 100 mg kg-1, respectively. On the 7th day after the start of feeding, the drug concentrations in the plasma, muscle and the main tissues involved in egg formation, the liver, and ovary and oviducts (magnum and isthmus plus shell grand) were determined by high-performance liquid chromatography. Dietary sulphonamides were distributed throughout the above tissues. SQ was found at the highest concentration in all tissues, while the reverse was true for SDD. The ratio of SDD concentrations in the main tissues involved in egg formation to that in the plasma were greater than those for the other drugs.
  • High-performance liquid chromatographic procedure for routine residue monitoring of seven sulfonamides in milk, N Furusawa, K Kishida, FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 371(7), 1031 - 1033, Dec. 2001 , Refereed
    Summary:A simplified method for routine monitoring of 7 residual sulfonamides (SAs) (sulfadiazine (SDZ), sulfamerazine (SMR), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfadimethoxine (SDM), and sulfaquinoxaline (SQ)) in milk using high-performance liquid chromatography (HPLC) with a photodiode array detector is described. The spiked and blank samples were cleaned up by using an Ultrafree (R) -MC/PL centrifugal ultrafiltration unit. For determination/identification, a Mightysil (R) RP-4 GP column and a mobile phase of 25% (v/v) ethanol in water with a photodiode array detector were used. Average recoveries from milk samples spiked with 0.05. 0.1, 0.2, and 0.5 mug mL(-1) of each drug were > 82%. The inter- and intra-assay variabilities were 2.0-3.1%. The practical detection limits for 7 SAs were 0.005-0.02 mug mL(-1). The total time and amount of solvent required for the analysis of one sample were < 40 min and <6 mL of ethanol, respectively. No toxic solvents were used.
  • Matrix solid-phase dispersion extraction and high-performance liquid chromatographic determination of residual sulfonamides in chicken, K Kishida, N Furusawa, JOURNAL OF CHROMATOGRAPHY A, JOURNAL OF CHROMATOGRAPHY A, 937(1-2), 49 - 55, Dec. 2001
    Summary:Simultaneous determination of the six sulfonamides (SAs) sulfadiazine, sulfadimidine, sulfamonomethoxine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline in chicken using matrix solid-phase dispersion (MSPD) with neutral aluminium oxide as an MSPD sorbent and high-performance liquid chromatography (HPLC) is presented. In the present MSPD, six SAs could be isolated by only one step, elution with a 70% (v/v) aqueous ethanol solution, without the sorbent conditioning and the sorbent-tissue matrix washing. For the HPLC determination, a LiChrospher 100 RP-8 and a mixture of 1% acetic acid solution (pH 3.0, in water)-acetonitrile-N,N-dimethylformamide (78:22:5, v/v/v) as the mobile phase with a photodiode array detector were used. Average recoveries were greater than 87.6% with relative standard deviations between 0.5 and 8.6%. The total time and amount of solvent required for the analysis of one sample were <1.5 h and < 12 ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.
  • Increases in 1,5-anhydroglucitol levels in germinating amaranth seeds and in ripening banana, Y Konishi, K Hashima, K Kishida, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64(11), 2462 - 2465, Nov. 2000 , Refereed
    Summary:To examine whether 1,5-anhydroglucitol (AG) is derived from starch degradation in plant tissues, we colorimetrically measured AG contents of germinating amaranth seeds and ripening banana pulp. In both cases, as starch degradation proceeded, AG levels were significantly increased, but were 1,700-5,000 times lower than those of total soluble carbohydrates. alpha -1,4-Glucan lyase activity, which is measured by the 1,5-anhydrofructose (AF) liberated from non-reducing glucose residues of starch or glycogen, was too low to be detected in amaranth or banana by the 3,5-dinitrosali-cylic acid method. On the other hand, AF reductase, which reduces AF to AG, was detected in germinating amaranth seeds and banana pulp. Thus, the increases in AG levels are conceived to be derived from starch breakdown, although further investigation is needed to answer whether the starch degradation pathway via alpha -1,4-glucan lyase/AF reductase exists in plant tissues.

Conference Activities & Talks

  • In vivo optical imaging analysis of UCP-1 gene expression in brown adipose tissues of mice, captured aroma constituents of Sansho, a Japanese pepper, Kunihiro Kishida, 74th Annual Meeting of Japan Society of Nutrition and Food Science,   2020 05
  • Digestibility and prebiotic effects by resistant starch derived from potato starch in bolo-like baked products, Kunihiro Kishida, 74th Annual Meeting of Japan Society of Nutrition and Food Science,   2020 05
  • Plasma Rare Sugar Levels and the Effect on Plasma Glucose Levels in GLUT5-induced Rats Gavaged with Rare Sugar D-Sorbose, D-Tagatose, or D-Allose, Kunihiro Kishida Kazushi Yoshikawa Tadao Taguchi Ryo Tamaoki Tetsuo Iida Takako Yamada Yukiyasu Toyoda, EXPERIMENTAL BIOLOGY 2020,   2020 04
  • Heterogeneous distribution of ion transporters in enteroids enriched in different intestinal epithelial cell types, Sarah C. Pearce Kunihiro Kishida Arwa Al-Jawadi Reilly J. Shiarella Madelyn J. Auvinen Juan A. Flores Shiyan Yu Nan A. Gao Ronaldo P. Ferraris, EXPERIMENTAL BIOLOGY 2020,   2020 04
  • Zanthoxylum piperitum activates thermogenic gene expression and beige adipogenesis in white adipose tissues of mice, Hayato Ihara Ken Takeshima Akio Kadowaki Nobuki Gato Kunihiro Kishida Takashi Akamizu, EXPERIMENTAL BIOLOGY 2020,   2020 04
  • D-Sorbose, D-tagatose but not D-allose are absorbed via GLUT5 in the small intestine, Kunihiro Kishida, Kazushi Yoshikawa, Syoma Minami, Tadao Taguchi, Ryo Tamaoki, Tetsuo Iida, Takako Yamada, Yukiyasu Toyoda, Rare Sugar Congress2019,   2019 12
  • Comprehensive analysis of the effects of high-fat diet on protein abundance in the mouse liver using SWATH acquisition method, Kunihiro Kishida,   2019 07
  • Metabolism-dependent Induction by Fructose of Lipogenic Gene Expression Occurs in Mouse Enterocytes And Not in Other Cell Types, Arwa P Al-Jawadi, Chirag R. Patel, Reilly J Shiarella, Emmanuellie Romelus, Madelyn Auvinen, Sarah C Pearce, Kunihiro Kishida, Shiyan Yu, Nan Gao, Ronaldo P Ferraris, Experimental Biology 2019,   2019 04 01
  • Absorption of rare sugars in the small intestine, Kunihiro Kishida, 9th Federation of the Asian and Oceanian Physiological Societies Congress (FAOPS2019),   2019 03 29 , 招待有り
  • Comprehensive quantification of liver proteins in the high-fat induced-obese mouse using a SWATH acquisition method-Toward elucidation of molecular mechanism of functional food substances-, OOTA S, YAMAWAKI S, NISHIBATA T, AWAJI T, OOSEDO Y, SAKAUE A, KISHIDA K, NAGAI K, 質量分析総合討論会講演要旨集,   2018 05 01
  • D‐プシコースは消化管のGLUT5を介して吸収される, 岸田邦博, 飯田哲郎, 山田貴子, RONALDO Ferraris, 豊田行康, 日本栄養・食糧学会大会講演要旨集,   2018 04 27
  • 地域住民コホートにおける多価不飽和脂肪酸と血清中可溶性LOX‐1濃度との関連, 上杉弥優, 牟礼佳苗, 岸田邦博, 島袋美絵, 服部園美, 宮井信行, 内海みよ子, 竹下達也, 有田幹雄, 日本衛生学雑誌(Web),   2018 03
  • 白色脂肪細胞のベージュ化を伴うサンショウの抗肥満効果の解析, 井原勇人, 竹島健, 門脇昭夫, 山下雅礼, 大西沙代, 岸田邦博, 我藤伸樹, 赤水尚史, 機能性食品と薬理栄養,   2017 12 01
  • 和歌山県産サンショウの抗肥満効果の解析, 井原勇人, 竹島健, 門脇昭夫, 大西紗与, 岸田邦博, 我藤伸樹, 赤水尚史, 日本栄養・食糧学会大会講演要旨集,   2017 04 28
  • Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells,   2017 04
  • 白色脂肪細胞のベージュ化を伴うサンショウの抗肥満効果の解析, 井原勇人, 竹島健, 門脇昭夫, 山下雅礼, 大西紗与, 岸田邦博, 我藤伸樹, 赤水尚史, 日本生化学会大会(Web),   2017
  • 薬物治療を受けていない地域在住高血圧患者における梅酢ポリフェノールの降圧作用, TAKEMURA SHIGEKI, YOSHIMASU KOICHI, TSUNO KANAMI, KURODA MOTOSHI, KISHIDA KUNIHIRO, YANO FUMIKO, MITANI TAKAHIKO, ARITA MIKIO, MIYASHITA KAZUHISA, 和歌山医学,   2015 09 30
  • 薬物治療を受けていない高血圧患者における梅酢ポリフェノールの降圧作用―12週間の予備試験―, TAKEMURA SHIGEKI, YOSHIMASU KOICHI, FUKUMOTO HITOSHI, KITANO NAOMI, NISHIO NOBUHIRO, MURE KANAE, KISHIDA KUNIHIRO, YANO FUMIKO, MITANI TAKAHIKO, TAKESHITA TATSUYA, ARITA MIKIO, MIYASHITA KAZUHISA, 和歌山医学,   2015 09 30
  • 「露茜」を原料とする梅酒の色調改善に関する研究 III~酸化防止剤添加による色素安定性の向上について~, OHARA MINA, YUKIMOTO RYO, HORINISHI ASAKO, KISHIDA KUNIHIRO, OZAKI YOSHIHIKO, SHOJI TOSHIHIKO, 日本食品保蔵科学会大会講演要旨集,   2015 06 27
  • 果実由来の機能性成分によるメタボリックシンドローム予防および改善に関する研究, KISHIDA KUNIHIRO, 日本食品保蔵科学会大会講演要旨集,   2015 06 27
  • 「ウメ赤肉品種‘露茜’のフェノール性化合物の組成とα‐グルコシダーゼに対する阻害作用」, HORINISHI ASAKO, FUJIOKA YUUMI, HIGASHIDE KOHEI, TANI YOSHIKI, KISHIDA KUNIHIRO, OZAKI YOSHIHIKO, 日本食品保蔵科学会大会講演要旨集,   2015 06 27
  • ウメ‘露茜’果肉のフェノール性化合物の解析, HORINISHI ASAKO, YUKIMOTO RYO, TANI YOSHIKI, MORI HAJIME, KISHIDA KUNIHIRO, OZAKI YOSHIHIKO, 日本農芸化学会大会講演要旨集(Web),   2015 03 05
  • The effects of dietary cinnamic acid and p-coumaric acid on glucose and lipid metabolism in normal rats fed high-sucrose diet, Kunihiro Kishida, Ken Takeshima, Yoshihiko Ozaki, Hayato Ihara, Experimental Biology 2015,   2015 03
  • 「露茜」を原料とする梅酒の色調改善に関する研究 II~追熟果実を原料とした梅酒の色調について~, 雪本亮, 小原三奈, 堀西朝子, 岸田邦博, 庄司俊彦, 尾崎嘉彦, 日本食品保蔵科学会大会講演要旨集,   2014 06 28
  • 梅酢由来ポリフェノール調製物(UP)の構成化合物の解明, 堀西朝子, 葛巻里紗, 部屋雄一, 多中良栄, 三宅靖仁, 高垣昌史, 森一, 岸田邦博, 尾崎嘉彦, 日本農芸化学会大会講演要旨集(Web),   2014 03 05
  • 薬物治療を受けていない高血圧患者における梅酢ポリフェノールの降圧作用―8週までの経過報告―, 竹村重輝, 福元仁, 吉益光一, 北野尚美, 西尾信宏, 牟礼佳苗, 岸田邦博, 矢野史子, 三谷隆彦, 竹下達也, 有田幹雄, 宮下和久, 和歌山医学,   2013 06 30
  • 梅酢ポリフェノールの食後血糖値上昇抑制作用, 岸田邦博, 部屋雄一, 山崎祐大, 堀西朝子, 尾崎嘉彦, 日本食品保蔵科学会大会講演要旨集,   2013 06 15
  • 梅酢ポリフェノールの機能性とバイオアベイラビリティ, 岸田邦博, 日本栄養・食糧学会大会講演要旨集,   2013 04 , 招待有り
  • 梅酢ポリフェノールのヒトにおける降圧効果等に関する研究, 竹村重輝, 福元仁, 吉益光一, 服部園美, 北野尚美, 西尾信宏, 牟礼佳苗, 岸田邦博, 矢野史子, 三谷隆彦, 竹下達也, 有田幹雄, 宮下和久, 和歌山医学,   2012 12 31
  • 梅酢ポリフェノールの降圧作用に関する研究(第2報), 山崎晋平, 和木田直希, 部屋雄一, 岸田邦博, 岸岡史郎, 堀西朝子, 山西妃早子, 矢野史子, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2012 04 27
  • 梅酢ポリフェノールの降圧作用に関する研究(第3報), 部屋雄一, 和木田直希, 山崎晋平, 田中あゆみ, 岸田邦博, 岸岡史郎, 堀西朝子, 山西妃早子, 矢野史子, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2012 04 27
  • ウメ(Prunus mume S.)の抗疲労作用に関する研究, 矢野史子, 荒木章吾, 藤田勝也, 白木琢磨, 岸田邦博, 高田善浩, 青柳正信, 高橋斉, 山西妃早子, 福西伸一, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2012 04 27
  • 梅酢ポリフェノールの生体内動態に関する研究(第2報), 田中あゆみ, 岸田邦博, 堀西朝子, 矢野史子, 山西妃早子, 赤木友裕, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2012 04 27
  • SHRの血圧に及ぼす梅酢ポリフェノールの作用(第3報), 和木田直希, 部屋雄一, 岸田邦博, 岸岡史郎, 山崎晋平, 川端伴顕, 矢野史子, 堀西朝子, 山西妃早子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノール画分の化学的性質(第6報), 高垣昌史, 山西妃早子, 野村英作, 岸田邦博, 堀西朝子, 多中良栄, 森めぐみ, 前田育克, 赤木知裕, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅果実中の核の化学的性質について, 川端伴顕, 堀西朝子, 多中良栄, 森めぐみ, 前田育克, 高垣昌史, 山西妃早子, 赤木知裕, 岸田邦博, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノール画分の化学的性質(第5報), 岸田邦博, 田中あゆみ, 川端伴顕, 野村英作, 堀西朝子, 山西妃早子, 高垣昌史, 多中良栄, 森めぐみ, 前田育克, 赤木智裕, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノールの抗菌作用に関する研究, 中林良彦, 太田佳菜, 川端伴顕, 岸田邦博, 堀西朝子, 石井営次, 池本重明, 稲葉伸也, 山西妃早子, 赤木知祐, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノール画分の化学的性質(第4報), 堀西朝子, 川端伴顕, 岸田邦博, 多中良栄, 森めぐみ, 前田育克, 山西妃早子, 赤木知裕, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • SHRの血圧に及ぼす梅酢ポリフェノールの作用(第2報), 山崎晋平, 岸田邦博, 岸岡史郎, 堀西朝子, 矢野史子, 山西妃早子, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノールの消化管吸収に関する研究, 田中あゆみ, 岸田邦博, 川端伴顕, 矢野史子, 堀西朝子, 赤木友裕, 三谷隆彦, 日本農芸化学会大会講演要旨集(Web),   2012 03 05
  • 梅酢ポリフェノールの調製と品質評価, 石原理恵, 則藤真理子, 山西妃早子, 赤木知裕, 多中良栄, 森めぐみ, 堀西朝子, 岸田邦博, 三谷隆彦, 日本薬学会年会要旨集,   2012 03 05
  • ウメ(Prunus mume S.)の果実中のヒドロキシ桂皮酸誘導体について, 多中良栄, 森めぐみ, 小畑俊嗣, 堀西朝子, 岸田邦博, 川端伴顕, 山西妃早子, 赤木知裕, 矢野史子, 三谷隆彦, 和歌山医学,   2011 12 31
  • 梅酢ポリフェノールの生体内動態に関する研究(第1報), 田中あゆみ, 岸田邦博, 堀西朝子, 矢野史子, 山西妃早子, 赤木知裕, 三谷隆彦, 和歌山医学,   2011 12 31
  • 梅酢ポリフェノールの工業的製造法に関する検討, 川端伴顕, 堀西朝子, 岸田邦博, 矢野史子, 赤木知裕, 山西妃早子, 木村昭雄, 稲葉伸也, 綾野茂, 大江孝明, 根来圭一, 三谷隆彦, 和歌山医学,   2011 12 31
  • ウメ(Prunus mume S.)の果実中の抗酸化成分に関する研究(第6報), 堀西朝子, 多中良栄, 森めぐみ, 小畑俊嗣, 岸田邦博, 川端伴顕, 山西妃早子, 赤木知裕, 矢野史子, 三谷隆彦, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集,   2011 10 01
  • 梅酢ポリフェノールの工業的製造法に関する検討, 川端伴顕, 堀西朝子, 岸田邦博, 矢野史子, 赤木知裕, 山西妃早子, 木村昭雄, 稲葉伸也, 綾野茂, 大江孝明, 根来圭一, 三谷隆彦, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集,   2011 10 01
  • 梅酢ポリフェノールの生体内動態に関する研究(第1報), 田中あゆみ, 岸田邦博, 堀西朝子, 矢野史子, 山西妃早子, 赤木知裕, 三谷隆彦, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集,   2011 10 01
  • 梅酢ポリフェノールの工業的製造法に関する検討, 赤木知裕, 山西妃早子, 堀西朝子, 木村昭雄, 稲葉伸也, 綾野茂, 大江孝明, 根来圭一, 川端伴顕, 岸田邦博, 矢野史子, 三谷隆彦, 日本食品科学工学会大会講演集,   2011 09 09
  • マウスの強制遊泳に及ぼす梅酢ポリフェノールの効果, 矢野史子, 志賀勇介, 土田辰典, 岸田邦博, 白木琢磨, 山西妃早子, 赤木知裕, 高田善浩, 青柳正信, 高橋斉, 福西伸一, 三谷隆彦, 日本畜産学会大会講演要旨,   2011 08 26
  • テキストマイニングを用いた若年世代の食事内容分析, 吉田知未, 岸田邦博, 鈴木明子, 松原主典, 冨永美穂子, 日本家政学会大会研究発表要旨集,   2011 05 20
  • ウメ(Prunus mume S.)の果実中の抗酸化成分に関する研究(第4報), 堀西朝子, 川端伴顕, 木村好伸, 多中良栄, 森めぐみ, 小畑俊嗣, 山西妃早子, 赤木知裕, 岸田邦博, 矢野史子, 尾崎嘉彦, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2011 04 25
  • ウメ(Prunus mume S.)の果実中の抗酸化成分に関する研究(第5報), 山西妃早子, 堀西朝子, 川端伴顕, 多中良栄, 森めぐみ, 小畑俊嗣, 赤木知裕, 岸田邦博, 矢野史子, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2011 04 25
  • 梅酢ポリフェノールの降圧作用に関する研究, 山崎晋平, 矢野史子, 岸田邦博, 岸岡史郎, 堀西朝子, 赤木知裕, 山西妃早子, 三谷隆彦, 日本栄養・食糧学会大会講演要旨集,   2011 04 25
  • 梅酢ポリフェノール画分の化学的性質(第2報), 川端伴顕, 堀西朝子, 木村好伸, 森めぐみ, 多中良栄, 小畑俊嗣, 山西妃早子, 赤木知裕, 岸田邦博, 尾崎嘉彦, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 梅酢ポリフェノール画分の化学的性質(第3報), 木村好伸, 堀西朝子, 川端伴顕, 多中良栄, 森めぐみ, 小畑俊嗣, 山西妃早子, 赤木知裕, 岸田邦博, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集,   2011 03 05
  • SHRの血圧に及ぼす梅酢ポリフェノールの作用, 山崎晋平, 島村将理, 矢野史子, 岸田邦博, 岸岡史郎, 堀西朝子, 赤木知裕, 山西妃早子, 三谷隆彦, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 梅酢ポリフェノールのマウスにおける血中動態, 岸田邦博, 田中あゆみ, 林佳世子, 堀西朝子, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集,   2011 03 05
  • 梅酢ポリフェノールの大量製造と安定性の検討, 堀西朝子, 赤木知裕, 木村昭雄, 川端伴顕, 木村好伸, 田口あや, 山西妃早子, 稲葉伸也, 大江孝明, 根来圭一, 岸田邦博, 矢野史子, 三谷隆彦, 日本農芸化学会大会講演要旨集,   2011 03 05
  • マウスにおけるクロロゲン酸およびフェルラ酸の経口投与後の血中動態, 岸田邦博, 田中あゆみ, 林佳世子, 矢野史子, 三谷隆彦, 和歌山医学,   2010 12 31
  • 若年世代の写真撮影された食事内容分析による食物摂取状況と身体組成,食生活を含めた生活習慣との関連, 吉田知未, 岸田邦博, 鈴木明子, 松原主典, 冨永美穂子, 日本家政学会大会研究発表要旨集,   2010 05 20
  • ピアフードサポートシステム構築に向けた大学生への食生活支援の試み, 冨永美穂子, 上田成一, 武藤慶子, 岸田邦博, 大曲勝久, 正木基文, 長崎県立大学看護栄養学部紀要,   2010 03
  • アマランサス粉置換スポンジケーキの性状と嗜好性, 永留史佳, 岸田邦博, 冨永美穂子, 日本調理科学会大会研究発表要旨集,   2010
  • ミトコンドリア遺伝子多型と性別による精神的健康度への交互作用, 岸田邦博, 冨永美穂子, 松原主典, 鈴木明子, 田口雅徳, 野口正憲, 綱分憲明, 四童子好廣, 日本人類遺伝学会大会プログラム・抄録集,   2009
  • 脂肪酸結合タンパク質2遺伝子多型が栄養素摂取量に与える影響, 岸田邦博, 青山みさ子, 冨永美穂子, 正木基文, 四童子好廣, 日本人類遺伝学会大会プログラム・抄録集,   2008
  • ビーズ式細胞破壊装置を用いた養殖魚からのテトラサイクリン系抗生物質の分析, 岸田邦博, 上田成一, 日本食品衛生学会学術講演会講演要旨集,   2007 10 01
  • 鶏卵に残留するサルファ剤及びアセチル代謝物の環境に優しい分析法, 岸田邦博, 日本食品化学学会総会・学術大会講演要旨集,   2006 06 15
  • 畜産食品中における残留サルファ剤および主要代謝物の同時分析法, 岸田邦博, 古沢直人, 日本食品衛生学会学術講演会講演要旨集,   2004 11 11
  • 血しょう中sulfamonomethoxine,sulfadimethoxineおよびhydroxy/N4‐acetyl代謝物の同時分析法, 岸田邦博, 古沢直人, 日本食品化学学会総会・学術大会講演要旨集,   2004 06 17
  • 有機溶媒フリーの血しょう中sulfamonomethoxine,sulfadimethoxineおよびN4‐acetyl代謝物の同時分析法, 岸田邦博, 古沢直人, 日本食品衛生学会学術講演会講演要旨集,   2004 04 20
  • 牛乳中に残留するsulfamonomethoxine,sulfadimethoxineおよびN4‐acetyl代謝物の同時HPLC簡易分析法, 岸田邦博, 古沢直人, 日本食品衛生学会学術講演会講演要旨集,   2003 04 20

Misc

  • Metabolic changes caused by the interaction between fructose and fatty acids, Kunihiro Kishida, Medical Science Digest, 46, 3, 34, 35,   2020 03
  • SWATH質量分析法による高脂肪食誘導肥満マウスの肝臓の定量プロテオミクス, 太田汐海, 西端智也, 山脇瑳也花, 淡路智貴, 大世渡勇紀, 阪上綾花, 岸田邦博, 永井宏平, 永井宏平, Memoirs of the Faculty of Biology-Oriented Science and Technology of Kindai University, 91回, 42, [3P, 247],   2018 09 , http://jglobal.jst.go.jp/public/201802223056474152
  • 「露茜」を原料とする梅酒の色調改善に関する研究 III~酸化防止剤添加による色素安定性の向上について~, 小原三奈, 雪本亮, 堀西朝子, 岸田邦博, 尾崎嘉彦, 庄司俊彦, 日本食品保蔵科学会大会講演要旨集, 64th, 15,   2015 06 27 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201502218432792620
  • p-クマル酸の機能性と吸収性に関するin vivo評価, KISHIDA KUNIHIRO, 食品加工技術, 35, 1, 1, 6,   2015 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502212074726026
  • 「露茜」を原料とする梅酒の色調改善に関する研究 II~追熟果実を原料とした梅酒の色調について~, 雪本亮, 小原三奈, 堀西朝子, 岸田邦博, 庄司俊彦, 尾崎嘉彦, 日本食品保蔵科学会大会講演要旨集, 63rd, 48,   2014 06 28 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201402298253673592
  • 食物摂取頻度調査票および写真撮影された食事調査法による栄養素等ならびに食品群別摂取量算出値の比較, 冨永美穂子, 高木千穂, 徳永真紀子, 岸田邦博, 長崎県立大学看護栄養学部紀要, 12, 19, 29,   2014 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402243982195855
  • 梅果汁成分による抗疲労効果(第2報), 佐藤夏海, 高橋斉, 坂井田和裕, 高田善浩, 矢野史子, 荒木章吾, 藤田勝也, 白木琢磨, 志賀勇介, 岸田邦博, 山西妃早子, 福西伸一, 三谷隆彦, 果汁協会報, 656, 1, 8,   2013 04 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302221354968470
  • 梅酢ポリフェノール抽出物の安全性の検討, 志賀勇介, 土田辰典, 原雄大, 岸田邦博, 前田正信, 宮下和久, 藤原真紀, 山西妃早子, 矢野史子, 三谷隆彦, Mem Fac Biol Oriented Sci Technol Kinki Univ, 28, 31, 40,   2011 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102289548586305
  • Attempt of Dietary Education to University Students towards Construction of Peer Food Support System, TOMINAGA Mihoko, UEDA Seiichi, MUTO Keiko, KISHIDA Kunihiro, OMAGARI Katsuhisa, MASAKI Motofumi,   2010 03 , http://hdl.handle.net/10561/657
  • Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells, Kunihiro Kishida, Sarah Pearce, Shiyan Yu, Nan Gao, Ronaldo Ferraris, FASEB JOURNAL, 31,   2017 04
  • The Life and Death of Differentiated and Dedifferentiated Intestinal Absorptive and Secretory Cells, Sarah C. Pearce, Kunihiro Kishida, Shiyan Yu, Nan Gao, Ronaldo P. Ferraris, FASEB JOURNAL, 31,   2017 04
  • The Effects of Dietary Cinnamic Acid and p-coumaric Acid on Glucose and Lipid Metabolism in Normal Rats Fed a High-Sucrose Diet, Kunihiro Kishida, Ken Takeshima, Yoshihiko Ozaki, Hayato Ihara, FASEB JOURNAL, 29,   2015 04

Research Grants & Projects

  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Research for stability of solubilyzing polyphenols with beta-1,3-glucan, The solubility improvement of hydroxycinnamic acids was investigated with beta-1,3-1,6-glucan from Aureobasidium pullulans. As a model compound, p-coumaric acid was evaluated for solubility with the beta glucan and their intestinal absorption. In addition, improvement of productivity also achieved through the optimization of cultivation conditions and mutation of the original strain K-1. The solubility of p-coumaric acid and caffeic acid were increased 1.5 and 3 times higher, respectively, by addition of beta glucan, which was renatured by the alkaline treatment. Moreover, its productivity was so enhanced and a unique albino mutant was obtained. On the other hand, the urinary excretion of p-coumaric acid did not change significantly using renatured beta glucan as a dispersant after single oral administration to rats. This suggests that beta glucan did not inhibit the absorption of p-coumaric acid. Detaild effect through the above p-coumaric acid solution will be continued.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Identification and functional analysis of food ingredients derived from regional goodies by in vivo imaging technique, 1. Examined the effects of hydroxycinnamic acids on gene expression of adipokines in 3T3-L1 adipocytes. . Among them, caffeic acid significantly reduced resistin, which causes insulin resistance state, gene expression dose dependently. In addition, caffeic acid also reduced resistin-reporter gene expression in stable adipocytes. After transplantation of these stable cells into inguinal fat pads of nude mice, administration of caffeic acid also reduced the reporter-gene expression in vivo. 2. We have examined effects of an another compound (patent pending) derived from regional goodies on high fat diet (HFD)-induced weight gain. C57BL/6 mice fed an HFD with this compound (3% w/w) significantly leaded to a reduced weight gain only after 1-week period compared with HFD-fed mice, and leaded to an induction of UCP-1 gene expression in inguinal fat pads.
  • Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Effects of polymorphisms on genes associated with lifestyle related disease on eating behaviors and postprandial physiological parameters, Association among common SNPs, eating behaviors, physiological parameters was investigated. The Ala54Thr polymorphism in the FABP2 gene led to higher intake of major nutrients, and there was an interactive effect of the mtDNA A10398G polymorphism and sex on anxiety and obsession in Japanese students. The interaction was observed between VEGF receptor-2 gene polymorphisms(rs1870377) and dietary patterns(meat, rice and noodles diet) on HbA1c and relative cholesterol levels(TC/HDLC) in Chinese Malaysian adults.