KINDAI UNIVERSITY


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MITANI Tasuku

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FacultyDepartment of Genetic Engineering / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
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Commentator Guidehttps://www.kindai.ac.jp/meikan/489-mitani-tasuku.html
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Last Updated :2020/11/25

Education and Career

Education

  •  - 1991 , Kyoto University, Graduate School of Agriculture
  •  - 1986 , Kyoto University, Faculty of Agriculture

Academic & Professional Experience

  •   2018 04 ,  - 現在, Professor, Faculty of Biology-Oriented Science and Technology, Kindai University
  •   2011 04 ,  - 2018 03 , Professor, Institute of Advanced Technology, Kindai University
  •   2002 11 ,  - 2014 02 , Director, Gene Control Corporation
  •   2007 04 ,  - 2011 03 , Associate Professor, Institute of Advanced Technology, Kinki University
  •   2005 04 ,  - 2007 03 , Associate Professor, Institute of Advanced Technology, Kinki University
  •   2002 04 ,  - 2005 03 , Institute of Advanced Technology, Kinki University
  •   1998 09 ,  - 1999 09 , Postdoctoral fellow, National Children's Medical Research Center
  •   1998 05 ,  - 1998 08 , Meiji Milk Product, Co. Ltd.
  •   1991 04 ,  - 1998 04 , Researcher, Institute of Health Science, Meiji Milk Product, Co. Ltd.

Research Activities

Research Interests

  • AGEs, embryonic development, fertilization, 3D-FISH, Embryonic stem cells, ABC transporter, Stem Cells, Nuclear Transfer

Published Papers

  • Records and distribution of the endangered Tokunoshima spiny rat Tokudaia tokunoshimensison Tokunoshima island, Japan, from 2005 to 2016, Takamichi Jogahara, Masataka Nakaya, Shigeru Ikemura, Chihiro Koshimoto, Shinsuke Sakamoto, Takuma Hashimoto, Tasuku MItani, Asato Kuroiwa, Fumio Yamada, Mammalian Science, Mammalian Science, 60(1), 105 - 116, Jan. 2020 , Refereed
  • Signs of biologucal activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging., Yamagata K, Nagai K, Miyamoto H, Anzai M, Kato H, Miyamoto K, Kurosaka S, Azuma R, Kolodeznikov II, Protopopov AV, Plotnikov VV, Kobayashi H, Kawahara-Miki R, Kono T, Uchida M, Shibata Y, Handa T, Kimura H, Hosoi Y, Mitani T, Matsumoto K, Iritani A, Scientific Reports, Scientific Reports, 9(1), 4050, Mar. 2019 , Refereed
  • Cryopreservation of Marchantia polymorpha spermatozoa., Togawa T, Adachi T, Harada D, Mitani T, Tanaka D, Ishizaki K, Kohchi T, Yamato KT, J. Plant Res., J. Plant Res., Jul. 2018 , Refereed
  • Ubiquitin-proteasome system modulates zygotic genome activation in early mouse embryos and influences full-term development., Higuchi C, Shimizu N, Shin SW, Morita K, Nagai K, Anzai M, Kato H, Mitani T, Yamagata K, Hosoi Y, Miyamoto K, Matsumoto K, J Reprod Dev., J Reprod Dev., 64(1), 65 - 74, Feb. 2018 , Refereed
  • Identification and characterization of the 5 '-flanking region of three mouse maternal genes (Histone H1oo, Nucleoplasmin 2, and Zygote arrest 1): Transcriptional activity in mouse oocytes, K. Tsunemoto, K. Matsumoto, M. Anzai, M. Hayakumo, T. Amano, T. Mitani, H. Kato, Y. Hosoi, K. Saeki, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 19(1), 257 - 258, 2007 , Refereed
  • In vitro culture of CD9- and α6-integrin-expressing cells enriched by magnetic cell sorting from cryptorchid adult and pup testes in mice., Mitani T, Ozaki Y, Tanaka Y, Takeuchi A, Saeki K, Kato H, Matsumoto K, Hosoi Y, Iritani A, Mem. Inst. Adv. Technol., Kinki Univ., Mem. Inst. Adv. Technol., Kinki Univ., 11, 23 - 34, Mar. 2006
  • In vitro culture of CD9-expressing cells enriched by magnetic cell sorting from testes of cryptorchid adult and pup in mice., Mitani T, Tanaka Y, Ozaki Y, Saeki K, Kato H, Matsumoto K, Hosoi Y, Iritani A, Reprod. Fertil. Develop., Reprod. Fertil. Develop., 18(1), 177 - 178, Jan. 2006 , Refereed
  • Analysis of global DNA methylation in bovine spermatozoa., Kato H, Kishimoto M, Mitani T, Matsumoto K, Saeki K, Hosoi Y, Iritani A, Reprod. Fertil. Develop., Reprod. Fertil. Develop., 18(1), 260 - 261, Jan. 2006 , Refereed
  • Effects of ethanol treatment after intracytoplasmic sperm injection (ICSI) on sperm aster formation and the microtubule organization of bovine oocytes., Fujinami N, Hosoi Y, Kato H, Mitani T, Matsumoto K, Saeki K, Hosoi Y, Iritani A, Reprod. Fertil. Develop., Reprod. Fertil. Develop., 17(1), 307, Jan. 2005 , Refereed
  • Methylation of the 5'-upstream region of the H19 gene in mouse somatic cell, gametes, wild type and androgenetic ES cells, Kato H, Murakami H, Kawasumi M, Kunieda T, Okuno M, Kishimoto M, Soma M, Iwai D, Anzai M, Mitani T, Matsumoto K, Saeki K, Hosoi Y, Iritani A, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 17(1-2), 261 - 262, 2005 , Refereed
  • Characterization of bovine early G1 cells and in vitro development of embryos reconstituted with the cells., Kasamatsu A, Saeki K, Tamari T, Shirouzu K, Taniguchi S, Mitani T, Aoyagi Y, Urakawa M, Ideta A, Matsumoto K, Hosoi Y, Iritani A, Reprod. Fertil. Develop., Reprod. Fertil. Develop., 17(1), 170, Jan. 2005 , Refereed
  • Analysis of DNA Methylation Pattern in Mouse Early Embryos by Bisulfite-Sequencing Method, Yuichi Unno, Miyuri Kawasumi, Kazuya Matsumoto, Tomoko Amano, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Hiromi Kato, Kazuya Matsumoto, Masayuki Anzai, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Journal of Mammalian Ova Research, Journal of Mammalian Ova Research, 22(4), 241 - 245, 2005
    Summary:Recently, with the acetylation of histone and the modification of chromatin structure, the methylation of cytosine residue within CpG dinucleotides in genomic DNA sequence attracts many researchers' attention as one of major epigenetic regulation systems of gene expression. There are several methods (immunofluorescence with 5-methylcytosine specific antibody and methylationsensitive restriction enzyme-PCR method, etc.) to analyze the methylation of cytosine residue. Bisulfitesequencing method, which is one of methods for analyzing the methylation of cytosine residue in genomic DNA sequence, has advantages of high sensitivity and analyzing the methylation of cytosine residue directly. In this note, the detailed procedure of bisulfite-sequencing method for mouse early Preimplantation embryos is described. © 2005, JAPANESE SOCIETY OF OVA RESEARCH. All rights reserved.
  • In vivo and in vitro differentiation of embryonic stem cells derived from parthenogenetic embryos in mice., Mitani T, Teramura T, Tada T, Hosoi Y, Iritani A, Reprod. Fertil. Develop., Reprod. Fertil. Develop., 16(1), 217, Jan. 2004 , Refereed
  • Rodent model of gene and cell transplantaton to fetal rat liver by in-utero manipulation for fetal therapy., Mitani T, Enosawa S, Takahashi N, Sakuragawa N, Suzuki S, Theriogenology, Theriogenology, 59(1), 537, Jan. 2003 , Refereed
  • Cell therapy by in-utero-manipulation, Terui K, Enosawa S, Mitani T, Onuma N, Suzuki S, Organ Biology, Organ Biology, 9(4), 333 - 342, Nov. 2002
  • Hepatocyte-like differentiation of amniotic epithelial cells and their possible application for regenerative medicine, Enosawa S, Mitani T, Suzuki S, Organ Biology, Organ Biology, 9(3), 265 - 274, Aug. 2002
  • Proliferation of alkaline phosphatase- positive cells under the culture conditions of primordial germ cells in rats., Mitani T, Takahashi N, Kawase E, Hashimoto K, Theriogenology, Theriogenology, 41(1), 258, Jan. 1994 , Refereed
  • Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes, Kohtaro Morita, Mikiko Tokoro, Yuki Hatanaka, Chika Higuchi, Haruka Ikegami, Kouhei Nagai, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Yoshitomo Taguchi, Kazuo Yamagata, Yoshihiko Hosoi, Kei Miyamoto, Kazuya Matsumoto, Journal of Reproduction and Development, Journal of Reproduction and Development, 64(2), 161 - 171, 2018 , Refereed
    Summary:Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
  • Visualization of the spatial arrangement of nuclear organization using three-dimensional fluorescence in situ hybridization in early mouse embryos: A new "EASI-FISH chamber glass" for mammalian embryos, Masataka Nakaya, Hideyuki Tanabe, Shingo Takamatsu, Misaki Hosokawa, Tasuku Mitani, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 63(2), 167 - 174, Apr. 2017 , Refereed
    Summary:The fertilized oocyte begins cleavage, leading to zygotic gene activation (ZGA), which re-activates the resting genome to acquire totipotency. In this process, genomic function is regulated by the dynamic structural conversion in the nucleus. Indeed, a considerable number of genes that are essential for embryonic development are located near the pericentromeric regions, wherein the heterochromatin is formed. These genes are repressed transcriptionally in somatic cells. Three-dimensional fluorescence in situ hybridization (3D-FISH) enables the visualization of the intranuclear spatial arrangement, such as gene loci, chromosomal domains, and chromosome territories (CTs). However, the 3D-FISH approach in mammalian embryos has been limited to certain repeated sequences because of its unfavorable properties. In this study, we developed an easy-to-use chamber device (EASI-FISH chamber) for 3D-FISH in early embryos, and visualized, for the first time, the spatial arrangements of pericentromeric regions, the ZGA-activated gene (Zscan4) loci, and CTs (chromosome 7), simultaneously during the early cleavage stage of mouse embryos by 3D-FISH. As a result, it was revealed that morphological changes of the pericentromeric regions and CTs, and relocation of the Zscan4 loci in CTs, occurred in the 1- to 4-cell stage embryos, which was different from those in somatic cells. This convenient and reproducible 3D-FISH technique for mammalian embryos represents a valuable tool that will provide insights into the nuclear dynamics of development.
  • Possible Role of ZPAC, Zygote-specific Proteasome Assembly Chaperone, During Spermatogenesis in the Mouse, Natsumi Shimizu, Kimihiro Ueno, Ena Kurita, Seung-Wook Shin, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Satoshi Kishigami, Hiromi Kato, Tasuku Mitani, Yoshihiko Hosoi, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 60(3), 179 - 186, Jun. 2014 , Refereed
    Summary:In the mammalian testis, the ubiquitin-proteasome system plays important roles in the process that promotes the formation of mature sperm. We recently identified zygote-specific proteasome assembly chaperone (ZPAC), which is specifically expressed in the mouse gonads and zygote. ZPAC mediates a unique proteasome assembly pathway in the zygote, but the expression profile and function of ZPAC in the testis is not fully understood. In this study, we investigated the possible role of ZPAC during mouse spermatogenesis. First, we analyzed the expression of ZPAC and 20S proteasome subunit alpha 4/PSMA7 in the adult mouse testis. ZPAC and alpha 4 were expressed in spermatogonia, spermatocytes, and round spermatids. In elongating spermatids, ZPAC was expressed until step 10, whereas expression of alpha 4 persisted until step 12. We then examined the expression profile of ZPAC and alpha 4 in a mouse model of experimental unilateral cryptorchidism. Consistent with appearance of morphologically impaired germ cells following cryptorchidism, the ZPAC protein level was significantly decreased at 4 days post induction of experimental cryptorchidism (D4) compared with the intact testis, although the amount of alpha 4 protein persisted at least until D10. Moreover, intense ZPAC staining was co-localized with staining of annexin V, an early indicator of apoptosis in mammalian cells, in germ cells of cryptorchid testis, but ZPAC was also expressed in germ cells showing no detectable expression of annexin V. These results suggest that ZPAC plays a role during spermatogenesis and raises the possibility that 20S proteasome mediated by ZPAC may be involved in the regulation of germ cell survival during spermatogenesis.
  • Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei, Takayuki Yamochi, Yuta Kida, Noriyoshi Oh, Sei Ohta, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Satoshi Kishigami, Tasuku Mitani, Kazuya Matsumoto, Kazuhiro Saeki, Makoto Takenoshita, Akira Iritani, Yoshihiko Hosoi, ZYGOTE, ZYGOTE, 21(4), 358 - 366, Nov. 2013 , Refereed
    Summary:Interspecies somatic cell nuclear transfer (ISCNT) has been proposed as a technique to produce cloned offspring of endangered species as well as to investigate nucleus-cytoplasm interactions in mammalian embryo. However, it is still not known which embryo culture medium is optimal for ISCNT embryos for the nuclear donor or the oocyte recipient. We assessed the effects of the culture medium on the developmental competence of the ISCNT embryos by introducing cynomolgus monkey (Macaca fascicularis) fibroblast nuclei into enucleated rabbit (Oryctolagus cuniculus) oocytes (monkey-rabbit embryo). The monkey-rabbit ISCNT embryos that were cultured in mCMRL-1066 developed to the blastocyst stage, although all monkey-rabbit ISCNT embryos cultured in M199 were arrested by the 4-cell stage. When monkey-rabbit ISCNT and rabbit-rabbit somatic cell nuclear transfer (SCNT) embryos were cultured in mCMRL-1066, the blastocyst cell numbers of the monkey-rabbit ISCNT embryos corresponded to the cell numbers of the control rabbit-rabbit SCNT embryos, which were produced from a rabbit fibroblast nucleus and an enucleated rabbit oocyte. In addition, the presence of mitochondria, which were introduced with monkey fibroblasts into rabbit recipient cytoplasm, was confirmed up to the blastocyst stage by polymerase chain reaction (PCR). This study demonstrated that: (1) rabbit oocytes can reprogramme cynomolgus monkey somatic cell nuclei, and support preimplantation development; (2) monkey-rabbit ISCNT embryos developed well in monkey culture medium at early embryonic developmental stages; (3) the cell number of monkey-rabbit ISCNT embryos is similar to that of rabbit-rabbit SCNT embryos; and (4) the mitochondrial fate of monkey-rabbit ISCNT embryos is heteroplasmic from the time just after injection to the blastocyst stage that has roots in both rabbit oocytes and monkey fibroblasts.
  • GSE Is a Maternal Factor Involved in Active DNA Demethylation in Zygotes, Yuki Hatanaka, Natsumi Shimizu, Satoshi Nishikawa, Mikiko Tokoro, Seung-Wook Shin, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Yoshihiko Hosoi, Satoshi Kishigami, Kazuya Matsumoto, PLOS ONE, PLOS ONE, 8(4), e60205, Apr. 2013 , Refereed
    Summary:After fertilization, the sperm and oocyte genomes undergo extensive epigenetic reprogramming to form a totipotent zygote. The dynamic epigenetic changes during early embryo development primarily involve DNA methylation and demethylation. We have previously identified Gse (gonad-specific expression gene) to be expressed specifically in germ cells and early embryos. Its encoded protein GSE is predominantly localized in the nuclei of cells from the zygote to blastocyst stages, suggesting possible roles in the epigenetic changes occurring during early embryo development. Here, we report the involvement of GSE in epigenetic reprogramming of the paternal genome during mouse zygote development. Preferential binding of GSE to the paternal chromatin was observed from pronuclear stage 2 (PN2) onward. A knockdown of GSE by antisense RNA in oocytes produced no apparent effect on the first and second cell cycles in preimplantation embryos, but caused a significant reduction in the loss of 5-methylcytosine (5 mC) and the accumulation of 5-hydroxymethylcytosine (5 hmC) in the paternal pronucleus. Furthermore, DNA methylation levels in CpG sites of LINE1 transposable elements, Lemd1, Nanog and the upstream regulatory region of the Oct4 (also known as Pou5f1) gene were clearly increased in GSE-knockdown zygotes at mid-pronuclear stages (PN3-4), but the imprinted H19-differential methylated region was not affected. Importantly, DNA immunoprecipitation of 5 mC and 5 hmC also indicates that knockdown of GSE in zygotes resulted in a significant reduction of the conversion of 5 mC to 5 hmC on LINE1. Therefore, our results suggest an important role of maternal GSE for mediating active DNA demethylation in the zygote.
  • EFFECT OF Dnmt1p mRNA KNOCK DOWN ON Dnmt1 PROTEIN TRANSLATION IN MOUSE TESTIS, H. Kato, R. Kitamura, H. Yamaguchi, Y. Numata, T. Kijima, M. Anzai, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 24(1), 202 - 202, 2012 , Refereed
  • In Vivo Application of an RNAi Strategy for the Selective Suppression of a Mutant Allele, Takayuki Kubodera, Hiromi Yamada, Masayuki Anzai, Shinga Ohira, Shigefumi Yokota, Yukihiko Hirai, Hideki Mochizuki, Takashi Shimada, Tasuku Mitani, Hidehiro Mizusawa, Takanori Yokota, HUMAN GENE THERAPY, HUMAN GENE THERAPY, 22(1), 27 - 34, Jan. 2011 , Refereed
    Summary:Gene therapy for dominantly inherited diseases with small interfering RNA (siRNA) requires mutant allele-specific suppression when genes in which mutation causes disease normally have an important role. We previously proposed a strategy for selective suppression of mutant alleles; both mutant and wild-type alleles are inhibited by most effective siRNA, and wild-type protein is restored using mRNA mutated to be resistant to the siRNA. Here, to prove the principle of this strategy in vivo, we applied it to our previously reported anti-copper/zinc superoxide dismutase (SOD1) short hairpin RNA (shRNA) transgenic (Tg) mice, in which the expression of the endogenous wild-type SOD1 gene was inhibited by more than 80%. These shRNA Tg mice showed hepatic lipid accumulation with mild liver dysfunction due to downregulation of endogenous wild-type SOD1. To rescue this side effect, we generated siRNA-resistant SOD1 Tg mice and crossed them with anti-SOD1 shRNA Tg mice, resulting in the disappearance of lipid accumulation in the liver. Furthermore, we also succeeded in mutant SOD1-specific gene suppression in the liver of SOD1(G93A) Tg mice, a model for amyotrophic lateral sclerosis, using intravenously administered viral vectors. Our method may prove useful for siRNA-based gene therapy for dominantly inherited diseases.
  • Competence of an artificial bent DNA as a transcriptional activator in mouse ES cells, Jun-ichi Tanase, Tasuku Mitani, Koji Udagawa, Jun-ichi Nishikawa, Takashi Ohyama, MOLECULAR BIOLOGY REPORTS, MOLECULAR BIOLOGY REPORTS, 38(1), 37 - 47, Jan. 2011 , Refereed
    Summary:Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. However, their potency in transgene activation in embryonic stem (ES) cells has not been examined. T20 is an artificial curved DNA of 180 bp that serves as a transcriptional activator. We investigated the effect of T20 on transcription in mouse ES cell lines or hepatocytes differentiated from them. We established 10 sets of cell lines each harboring a single copy of the reporter construct. Each set comprised a T20-harboring cell line and a T20-less control cell line. Analyses showed that in ES cells and in hepatocytes originating from these cells, T20 both activated and repressed transcription in a manner that was dependent on the locus of reporter. The present and previous studies strongly suggest that in cells that have a strict gene regulation system, transcriptional activation by T20 occurs only in a transcriptionally active locus in the genome.
  • Deposition of Acetylated Histones by RNAP II Promoter Clearance May Occur at Onset of Zygotic Gene Activation in Preimplantation Mouse Embryos, Mikiko Tokoro, Seung-Wook Shin, Satoshi Nishikawa, Hyang-Heun Lee, Yuki Hatanaka, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Masayuki Anzai, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56(6), 607 - 615, Dec. 2010 , Refereed
    Summary:We investigated the contribution of phosphorylated RNA polymerase II (RNAP II) and dynamic epigenetic changes to the onset of minor zygotic gene activation (ZGA) Using immunofluorescence staining, we observed that the nuclear Localization of RNAP IT was initiated by 6 hours post insemination (hpi), whereas RNAP II phosphorylated at serine residue 5 of the carboxyl-terminal domain (CTD) was localized by 9 hpi, and then RNAP II phosphorylated at serine residue 2 of the CTD was localized in the nucleus of embryos by 12 hpi In a transient gene expression assay using a plasmid reporter gene (p beta-actin/luciferase+/SV40) injected during 6-9 hpi into the male pronucleus, the luciferase+gene was actively transcribed and translated by 13 and 15 hpi, respectively, indicating that a transcriptionally silent state persisted for it least 4 hours after injection We found that the methylation status in the chicken beta-actin promoter region of the plasmid reporter gene may not be associated with the transcriptionally silent state before minor ZGA Exposure to trichostatin A did not induce premature expression of the silent reporter gene injected into 1-cell embryos containing histone deacetylase activity and did not affect the amount of luciferase produced per embryo Acetylated histone H3 lysine 9/14 and acetylated histone H4 lysine 12 and 16 were enriched preferentially in the injected reporter gene at least until 13 hpi, which coincided with the transcriptionally active state Taken together, these results suggest that deposition 01 selectively acetylated histones onto the chromatin of 1-cell embryos functions together with transcriptional elongation by RNAP II and that this sequential chromatin remodeling is involved in the molecular mechanism associated with the onset of minor ZGA in the preimplantation mouse embryo
  • Inhibition of the Ubiquitin-proteasome System Leads to Delay of the Onset of ZGA Gene Expression, Seung Wook Shin, Mikiko Tokoro, Satoshi Nishikawa, Hyang-Heun Lee, Yuki Hatanaka, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56(6), 655 - 663, Dec. 2010 , Refereed
    Summary:In mammalian oocytes, the ubiquitin-proteasome system (UPS) is suggested to play important roles in oocyte meiosis resumption, spindle assembly, polar body emission and pronuclear formation by regulating cyclin B1 degradation Ho Never, little is known about the direct relationship between zygotic gene activation (ZGA) and degradation of maternal proteins Here, we investigated the role of the UPS in the onset of ZGA in early mouse embryo First, we found degradation of cyclin B1 protein in fertilized oocytes at 1 hpi by western blot analysis and used the se oocytes throughout this study Subsequently, we determined optimal experimental conditions for transient inhibition of proteasomal activity by specific and reversible proteasomal inhibitor MG132 in the Cl phase of the first cell cycle Under the selected optimal conditions, we subjected transient MG132-treated embryos to reverse transcription (RT)-PCR analysis of expression of four ZGA genes, i e, the hsp70 1, MuERV-L, eif-1a and zscan4d genes As a result, we found that onset of expression of the four examined ZGA genes was delayed m both normally developed 2-cell embryos and arrested 1-cell embryos Our results indicate that proteasomal degradation of proteins by the UPS plays a pivotal role in the molecular mechanisms of ZGA in early mouse embryos
  • Expression analysis of circadian genes in oocytes and preimplantation embryos of cattle and rabbits, Tomoko Amano, Kaori Tokunaga, Reiko Kakegawa, Ayaka Yanagisawa, Atsushi Takemoto, Atsuhiro Tatemizo, Tatsuya Watanabe, Yuki Hatanaka, Akinori Matsushita, Masao Kishi, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, ANIMAL REPRODUCTION SCIENCE, ANIMAL REPRODUCTION SCIENCE, 121(3-4), 225 - 235, Sep. 2010 , Refereed
    Summary:We previously showed that circadian genes clock, bmal1, cry1, cry2, per1, and per2 are expressed and function as maternal mRNA regulating events in the oocytes and preimplantation embryos of mice. Recent evidence indicates however that either or both expression profiles of circadian genes in some tissues, and transcript sequences of circadian genes, differ to generate the physiological differences between diurnal and nocturnal species. We therefore investigated the expression profiles of circadian genes in oocytes and preimplantation embryos of species other than mice, namely cattle and rabbits, representing diurnal and nocturnal species, respectively, and determined the protein sequences of circadian genes in these species. Quantitative real-time PCR revealed that all circadian genes considered in this study were present in the oocytes and preimplantation embryos of both species, and the transcript amounts of clock, cry1 and per1 contained in oocytes were significantly higher than in preimplantation embryos of both species. The transcripts of clock, cry1, and per1 of cattle and rabbits were determined by primer walking, and functional domains in the estimated amino acid sequences were compared between cattle and rabbits and with those of humans and mice. The sequences of clock, cry1, and per1 in cattle and rabbits closely resembled those in mice (85-100% homologies), and no difference based on diurnality or nocturnality was observed. These findings suggest that circadian genes in the oocytes and preimplantation embryos of mammals fulfill the same functions across species as maternal mRNA. (C) 2010 Elsevier B.V. All rights reserved.
  • Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice, Yuta Onodera, Takeshi Teramura, Madoka Ozawa, Toshiyuki Takehara, Tasuku Mitani, Masayuki Anzai, Norimasa Sagawa, Chiaki Hamanishi, Yoshihiko Hosoi, Kanji Fukuda, THERIOGENOLOGY, THERIOGENOLOGY, 74(1), 135 - 145, Jul. 2010 , Refereed
    Summary:Recent studies have illustrated multiple differentiation potentials of embryonic stem cells (ESCs), derived from parthenogenetic embryos, to various kinds of cells (all three embryonic germ layers). However, differentiation diversity of the parthenogenetic ESCs (PgESCs) in vivo remains to be elucidated. In the present study, we established mouse PgESC-lines and observed their contribution diversity in vivo by producing chimeric mice using embryos possessing single nucleotide polymorphisms of mitochondrial DNA (mtDNA) as hosts. Based on southern blot analysis using specific probes to detect the SNPs on mtDNA, PgESC-derived mtDNA were contained in many organs such as brain, lung, and heart of the chimeric mouse. We concluded that PgESCs contributed to various internal organs in vivo, and that they were also stably maintained in adult animals. (C) 2010 Elsevier Inc. All rights reserved.
  • FIBROBLAST GROWTH FACTOR 4 PROMOTES THE DEVELOPMENT OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS IN MICE, T. Mitani, M. Morita, M. Anzai, Y. Nishiyama, K. Moriki, H. Kawamura, H. Kato, K. Saeki, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 22(1), 193 - 194, 2010 , Refereed
  • RECOVERY OF CELL NUCLEI FROM 15 000-YEAR-OLD MAMMOTH TISSUES AND INJECTION INTO MOUSE ENUCLEATED MATURED OOCYTES, H. Kato, M. Anzai, T. Mitani, M. Morita, Y. Nishiyama, A. Nakao, K. Kondo, P. A. Lazarev, T. Ohtani, Y. Shibata, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 22(1), 189 - 189, 2010 , Refereed
  • Recovery of cell nuclei from 15,000 years old mammoth tissues and its injection into mouse enucleated matured oocytes, Hiromi Kato, Masayuki Anzai, Tasuku Mitani, Masahiro Morita, Yui Nishiyama, Akemi Nakao, Kenji Kondo, Petr A. Lazarev, Tsuyoshi Ohtani, Yasuyuki Shibata, Akira Iritani, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 85(7), 240 - 247, Jul. 2009 , Refereed
    Summary:Here, we report the recovery of cell nuclei front 14,000-15,000 years old mammoth tissues and the injection of those nuclei into mouse enucleated matured oocytes by somatic cell nuclear transfer (SCNT). From both skin and muscle tissues, cell nucleus-like structures were successfully recovered. Those nuclei were then injected into enucleated oocytes and more than half of the oocytes were able to survive. Injected nuclei were not taken apart and remained its nuclear structure. Those oocytes did not show disappearance of nuclear membrane or premature chromosome condensation (PCC) at 1 hour after injection and did not form pronuclear-like structures at 7 hours after injection. As half of the oocytes injected with nuclei derived from frozen-thawed mouse bone marrow cells were able to form pronuclear-like structures, it might be possible to promote the cell cycle of nuclei from ancient animal tissues by suitable pre-treatment in SCNT. This is the first report of SCNT with nuclei derived from mammoth tissues.
  • Mouse Androgenetic Embryonic Stem Cells Differentiated to Multiple Cell Lineages in Three Embryonic Germ Layers In Vitro, Takeshi Teramura, Yuta Onodera, Hideki Murakami, Syunsuke Ito, Toshihiro Mihara, Toshiyuki Takehara, Hiromi Kato, Tasuku Mitani, Masayuki Anzai, Kazuya Matsumoto, Kazuhiro Saeki, Kanji Fukuda, Norimasa Sagawa, Yoshihiko Hosoi, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 55(3), 283 - 292, Jun. 2009 , Refereed
    Summary:The embryos of some rodents and primates can precede early development without the process of fertilization; however, they cease to develop after implantation because of restricted expressions of imprinting genes. Asexually developed embryos are classified into parthenote/gynogenote and androgenote by their genomic origins. Embryonic stem cells (ESCs) derived from asexual origins have also been reported. To date, ESCs derived from parthenogenetic embryos (PgESCs) have been established in some species, including humans, and the possibility to be alternative sources for autologous cell transplantation in regenerative medicine has been proposed. However, some developmental characteristics, which might be important for therapeutic applications, such as multiple differentiation capacity and transplantability of the ESCs of androgenetic origin (AgESCs) are uncertain. Here, we induced differentiation of mouse AgESCs and observed derivation of neural cells, cardiomyocytes and hepatocytes in vitro. Following differentiated embryoid body (EB) transplantation in various mouse strains including the strain of origin, we found that the EBs Could engraft in theoretically MHC-matched strains. Our results indicate that AgESCs possess at least two important characteristics, multiple differentiation properties in vitro and transplantability after differentiation, and suggest that they can also serve as a source of histocompatible, tissues for transplantation.
  • Abnormal DNA Methylation of the Oct-4 Enhancer Region in Cloned Mouse Embryos, Miyuri Kawasumi, Yuichi Unno, Toshiki Matsuoka, Megumi Nishiwaki, Masayuki Anzai, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Satoshi Kishigami, Kazuya Matsumoto, MOLECULAR REPRODUCTION AND DEVELOPMENT, MOLECULAR REPRODUCTION AND DEVELOPMENT, 76(4), 342 - 350, Apr. 2009 , Refereed
    Summary:Oct-4 is essential for normal embryonic development, and abnormal Oct-4 expression in cloned embryos contributes to cloning inefficiency. However, the causes of abnormal Oct-4 expression in cloned embryos are not well understood. As DNA methylation in regulatory regions is known to control transcriptional activity, we investigated the methylation status of three transcriptional regulatory regions of the Oct-4 gene in cloned mouse embryos-the distal enhancer (DE), the proximal enhancer (PE), and the promoter regions. We also investigated the level of Oct-4 gene expression in cloned embryos. Immunochemistry revealed that 85% of cloned blastocysts expressed Oct-4 in both trophectoderm and inner cell mass cells. DNA methylation analysis revealed that the PE region methylation was greater in cloned morulae than in normal morulae. However, the same region was less methylated in cloned blastocysts than in normal blastocysts. We found abnormal expression of de novo methyltransferase 3b in cloned blastocysts. These results indicate that cloned embryos have aberrant DNA methylation in the CpG sites of the PE region of Oct-4, and this may contribute directly to abnormal expression of this gene in cloned embryos.
  • Expression and Functional Analyses of Circadian Genes in Mouse Oocytes and Preimplantation Embryos: Cry1 Is Involved in the Meiotic Process Independently of Circadian Clock Regulation, Tomoko Amano, Akinori Matsushita, Yuki Hatanaka, Tatsuya Watanabe, Katsutaka Oishi, Norio Ishida, Masayuki Anzai, Tasuku Mitani, Hiromi Kato, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, BIOLOGY OF REPRODUCTION, BIOLOGY OF REPRODUCTION, 80(3), 473 - 483, Mar. 2009 , Refereed
    Summary:In mammals, circadian genes, Clock, Arntl (also known as Bmal1), Cry1, Cry2, Per1, Per2, and Per3, are rhythmically transcribed every 24 h in almost all organs and tissues to tick the circadian clock. However, their expression and function in oocytes and preimplantation embryos have not been investigated. In this study we found that the circadian clock may stop in mouse oocytes and preimplantation embryos. Real-time PCR analysis revealed the presence of transcripts of these genes in both oocytes and preimplantation embryos; however, their amounts did not oscillate every 24 h in one- to four-cell and blastocyst-stage embryos. Moreover, immunofluorescence analyses revealed that CLOCK, ARNTL, and CRY1 were localized similarly in the nuclei of germinal vesicle (GV) oocytes and one-cell- to four-cell-stage embryos. Because CRY1 is known to interact with the CLOCK-ARNTL complex to suppress transcription-promoting activity of the complex for genes such as Wee1, Cry2, Per1, Per2, and Per3 in cells having the ticking circadian clock, we hypothesized that if the circadian clock functions in GV oocytes and one-cell- to four-cell-stage embryos, CLOCK, ARNTL, and CRY1 might suppress the transcription of these genes in GV oocytes and one-cell- to 4-cell-stage embryos as well. As a result, knockdown of CRY1 in GV oocytes by RNA interference did not affect the transcription levels of Wee1, Cry2, Per1, Per2, and Per3, but it reduced maturation ability. Thus, it seems that circadian genes are not involved in circadian clock regulation in mouse oocytes and preimplantation embryos but are involved in physiologies, such as meiosis.
  • Silencing efficiency differs among tissues and endogenous microRNA pathway is preserved in short hairpin RNA transgenic mice, Hiroki Sasaguri, Tasuku Mitani, Masayuki Anzai, Takayuki Kubodera, Yuki Saito, Hiromi Yamada, Hidehiro Mizusawa, Takanori Yokota, FEBS LETTERS, FEBS LETTERS, 583(1), 213 - 218, Jan. 2009 , Refereed
    Summary:In short hairpin RNA (shRNA) transgenic mice, the tissue difference in gene silencing efficiency and oversaturation of microRNA (miRNA) pathway have not been well assessed. We studied these problems in our previously-reported anti-copper/zinc superoxide dismutase (SOD1) shRNA transgenic mice. Although there was a tissue difference (liver and skeletal muscle, >95%; central nervous system and lung, similar to 80%), the target gene silencing was systemic and our anti-SOD1 shRNA transgenic mice recapitulated the SOD1-null mice. Neither endogenous miRNAs nor their target gene levels were altered, indicating the preservation of endogenous miRNA pathways. We think that the shRNA transgenic mice can be utilized for gene analysis. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
  • Isolation and Culture of Rabbit Primordial Germ Cells, Ryo Kakegawa, Takeshi Teramura, Toshiyuki Takehara, Masayuki Anzai, Tasuku Mitani, Kazuya Matsumoto, Kazuhiro Saeki, Norimasa Sagawa, Kanji Fukuda, Yoshihiko Hosoi, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 54(5), 352 - 357, Oct. 2008 , Refereed
    Summary:Primordial germ cells (PGCs) are embryonic precursors of the gametes of adult animals and are considered stem cells of the germline. Since their proliferation in vitro correlates well with the schedule of developmental changes in vivo, they might be interesting research tools for genomic imprinting, germ-cell tumors and fertility. Furthermore, once primordial germ cells are separated and placed on a feeder layer with cytokines, they become Cultured pluripotent cell lines called embryonic germ (EG) cells. EG cells share several important characteristics with embryonic stem (ES) cells as they call also contribute to the germ line of chimeras. To investigate the characteristics of PGCs and establish rabbit EG (rEG) cells, we cultured rabbit PGCs (rPGCs) in vitro with various combinations of leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and forskolin oil inactivated mouse embryonic fibroblast (MEF) feeder layers. The present study found PGC proliferation in early cultures and induction of rEG-like colonies. These cells expressed pluripotent markers, such as alkaline phosphatase activity, OCT-4, Sox-2 and SSEA-1, in the undifferentiated state; however, the cells did not develop into a teratoma when injected into the kidney capsules of SCID mice, although the restricted differentiation potentials to neural cells were determined via embryoid body formation. From these characteristics and further characterization of the germ stem cell markers Vasa, SCP-1 and SCP-3, we suggested that these were hybrid cells with characteristics somewhere between PGC and EG cells.
  • Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone H1oo, Nucleoplasmin 2, and Zygote arrest 1) are required for oocyte-specific gene expression in the mouse, Kazunobu Tsunemoto, Masayuki Anzai, Toshiki Matsuoka, Mikiko Tokoro, Seung-Wook Shin, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Yoshihiko Hosoi, Kazuhiro Saeki, Akira Iritani, Kazuya Matsumoto, MOLECULAR REPRODUCTION AND DEVELOPMENT, MOLECULAR REPRODUCTION AND DEVELOPMENT, 75(7), 1104 - 1108, Jul. 2008 , Refereed
    Summary:We examined the promoter activities of three mouse maternal genes (H1oo, Npm2, and Zar1) in oocytes and pre-implantation embryos, and examined the promoters for cis-acting elements of 5'-flanking region to obtain the best promoter for inducing oocyte-specific gene expression. For the assay, we injected firefly luciferase gene constructs under the control of the promoters into the oocytes and embryos. Each promoter region showed transcriptional activity in oocytes, but not in fertilized embryos. Deletion analysis showed that a putative E-box region at position -72 of the H1oo promoter and at the -180 of the Npm2 promoter were required for basal transcriptional activity in oocytes. Moreover, a putative NBE motif (NOBOX DNA binding elements) (-1796) was shown to enhance basal transcriptional activity of the Npm2 promoter. Thus, the E-box and/or NBE may be key regulatory regions for the expression of the examined maternal genes (H1oo and Npm2) in growing mouse oocytes.
  • Identification of ZAG1, a novel protein expressed in mouse prei rn plantation, and its putative roles in zygotic genome activation, Toshiki Matsuoka, Manabu Sato, Mikiko Tokoro, Seung-Wook Shin, Atsuto Uenoyama, Kazunari Ito, Syuji Hitomi, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 54(3), 192 - 197, Jun. 2008 , Refereed
    Summary:We isolated a mouse cDNA, zag1 (zygotic gene activation-associated gene 1), that has an open reading frame of 1,728-bp encoding a protein of 66.2 kDa including both a bipartite nuclear targeting sequence and a P-loop motif containing nucleoside triphosphate hydrolase motifs. Northern blot analysis of mouse tissues showed that zag1 was widely expressed but was especially prominent in the ovary and testis. RT-PCR analysis of in vitro fertilized embryos showed that the abundance of zag1 transcripts in oocytes decreased after fertilization, and zag1 mRNA was detected at 15 h post insemination (hpi) in fertilized embryos indicating that the gene was expressed at the start of zygotic gene activation at the mouse 1-cell stage. The nuclear-localization of ZAG1 protein in mouse preimplantation embryos at 15 hpi was confirmed by both subcellular analysis of enhanced green fluorescent protein (EGFP)-tagged ZAG1 and immunocytochemical analysis with anti-ZAG1 antibody. Subsequently, using yeast two-hybrid screening, we identified U2 small nuclear ribonucleoprotein B (U2B"), which is associated with pre-mRNA splicing, as a putative interacting partner of ZAG1 protein. Furthermore, knockdown of zag1 expression by an antisense DNA plasmid induced arrest and/or delay of embryonic development in injected 1-cell embryos. These results suggest that ZAG1 may be closely associated with zygotic gene expression in mouse preimplantation embryos.
  • Production of cloned bovine embryos derived from amniotic cells of pregnant cows, S. Taniguchi, N. Hayashi, Y. Abe, D. Iwamoto, S. Kishigami, M. Kishi, H. Kato, T. Mitani, K. Matsumoto, Y. Hosoi, A. Iritani, Y. Nagao, K. Saeki, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 20(1), 110 - 110, 2008 , Refereed
  • Expression of transcription factors specific to the trophoblast lineage in mouse somatic nuclear transfer embryos, T. Mitani, M. Nishiwaki, M. Anzai, H. Kato, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 20(1), 103 - 104, 2008 , Refereed
  • Early development of reconstructed mouse embryos using bone marrow cells frozen without cryoprotectant, H. Kato, A. Nakao, M. Nishiwaki, M. Anzai, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 20(1), 100 - 100, 2008 , Refereed
  • Effects of trichostatin a on DNA methylation in cloned bovine embryos, D. Iwamoto, S. Kishigami, S. Taniguchi, Y. Abe, T. Matsui, A. Kasamatsu, A. Tatemizo, T. Mitani, H. Kato, K. Matsumoto, Y. Hosoi, T. Wakayama, A. Iritani, K. Saeki, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 20(1), 99 - 99, 2008 , Refereed
  • Abnormal distribution of chromosomes in the first division of nuclear transferred mouse embryos, Miyuri Kawasumi, Masayuki Anzai, Toshiyuki Takehara, Tasuku Mitani, Hiromi Kato, Kazuhiro Saeki, Akira Iritani, Kazuya Matsumoto, Yoshihiko Hosoi, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(3), 615 - 622, Jun. 2007 , Refereed
    Summary:The majority of somatic cell nuclear transferred (SCNT) embryos die before or after implantation. Many studies have focused on morphological remodeling of the donor nucleus and its associated cytoskeletal structures in the early events of nuclear transfer. However, little is known about the 2-cell stage of SCNT embryos after the first division. In this study, we compared the morphological status of chromosomal division during the 1-cell stage to the 2-cell stage in SCNT embryos with that in intracytoplasmic sperm injection (ICSI) embryos. The microtubules and cytoplasmic asters, which are related to chromatin segregation, disappeared at the pronuclear stage, although formation of the first mitotic spindle was normal in both the SCNT and ICSI embryos. However, nuclear fragmentation was observed in 30% of the 2-cell SCNT embryos and 12% of the 2-cell ICSI embryos. Nuclear fragmentation was present in both blastomeres of these embryos. No apoptotic DNA fragmentation was observed in TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) assays for either the SCNT or ICSI embryos. In both the SCNT and ICSI embryos, the distribution of chromosomes in the first mitotic spindle was disturbed during the process of division from the 1-cell stage to the 2-cell stage. These results suggest that loss of SCNT embryos just before or after implantation may be due to an abnormal chromosome distribution at the 2-cell stage.
  • Effects of trichostatin a on development of bovine somatic cell nuclear transfer embryos, D. Iwamoto, K. Saeki, S. Kishigami, A. Kasamatsu, A. Tatemizo, Y. Abe, S. Ikeda, S. Taniguchi, T. Mitani, H. Kato, K. Matsumoto, Y. Hosoi, T. Wakayama, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 19(1), 142 - 142, 2007 , Refereed
  • DNA methylation profiles of upstream elements of Oct-3/4 gene in in vitro fertilization (IVF) and somatic cell nuclear-transferred (SCNT) embryos, M. Kawasumi, Y. Unno, M. Nishiwaki, K. Matsumoto, M. Anzai, T. Amano, T. Mitani, H. Kato, K. Saeki, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 19(1), 143 - 143, 2007 , Refereed
  • Effect of aging on amounts of DNA methyltransferase mRNA in mouse spermatozoa, H. Kato, T. Koda, M. Kishimoto, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 19(1), 277 - 278, 2007 , Refereed
  • Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system, T. Mitani, T. Nagai, T. Masutani, H. Kato, K. Saeki, K. Matsumoto, Y. Hosoi, A. Iritani, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 19(1), 230 - 230, 2007 , Refereed
  • Primate embryonic stem cells proceed to early gametogenesis in vitro, Takeshi Teramura, Toshiyuki Takehara, Nobuyuki Kawata, Nahoko Fujinami, Tasuku Mitani, Makoto Takenoshita, Kazuya Matsumoto, Kazuhiro Saeki, Akira Iritani, Norimasa Sagawa, Yoshihiko Hosoi, Cloning and Stem Cells, Cloning and Stem Cells, 9(2), 144 - 156, 2007 , Refereed
    Summary:Embryonic stem cells (ESCs) of nonhuman primates are important for research into human gametogenesis because of similarities between the embryos and fetuses of nonhuman primates and those of humans. Recently, the formation of germ cells from mouse ESCs in vitro has been reported. In this study, we established cynomolgus monkey ES cell lines (cyESCs) and attempted to induce their differentiation into germ cells to obtain further information on the development of primate germ cells by observing the markers specific to germ cells. Three cyESCs were newly established and confirmed to be pluripotent. When the cells are induced to differentiate, the transcripts of Vasa and some meiotic markers were expressed. VASA protein accumulated in differentiated cell clumps and VASA-positive cells gathered in clumps as the number of differentiation days increased. In the later stages, VASA-positive clumps coexpressed OCT-4, suggesting that these cells might correspond to early gonocytes at the postmigration stage. Furthermore, meiosis-specific gene expression was also observed. These results demonstrate that cyESCs can differentiate to developing germ cells such as primordial germ cells (PGCs) or more developed gonocytes in our differentiation systems, and may be a suitable model for studying the mechanisms of primate germ cell development. © Mary Ann Liebert, Inc.
  • Increase of disease duration of amyotrophic lateral sclerosis in a mouse model by transgenic small interfering RNA, Takanori Yokota, Hiroki Sasaguri, Yuki Saito, Hiromi Yamada, Toshinori Unno, Yuki Yamamoto, Takayuki Kubodera, Masayuki Anzai, Tasuku Mitani, Hidehiro Mizusawa, Archives of Neurology, Archives of Neurology, 64(1), 145 - 146, Jan. 2007 , Refereed
  • Application of laser-assisted zona drilling to in vitro fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse, Masayuki Anzai, Megumi Nishiwak, Miho Yanagi, Tatsuyuki Nakashima, Takehito Kaneko, Yoshitomo Taguchi, Mikiko Tokoro, Seung-Wook Shin, Tasuku Mitani, Hiromi Kato, Kazuya Matsumoto, Naomi Nakagata, Akira Iritani, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(5), 601 - 606, Oct. 2006 , Refereed
    Summary:Development of assisted reproductive technologies is necessary to obtain fertilized oocytes in a subfertile transgenic mouse strain. Here, we showed the application of laser-assisted drilling of the zona pellucida to in vitro fertilization of cryopreserved mouse oocytes with sperm from subfertile transgenic mice (C57BL/6N-Tg(UCP/FAD2)U8 strain). After cryopreservation by vitrification, the recovery and survival rates of the zona-drilled mouse oocytes were 97% (97/100) and 94% (91/97), respectively. In vitro fertilization of the cryopreserved zona-drilled mouse oocytes with sperm from the subfertile transgenic mice was greatly facilitated (60%, 55/91) compared to that of the cryopreserved zona-intact mouse oocytes (11%, 81/768). In vitro fertilized embryos that developed to the 2-cell stage were again cryopreserved by vitrification, and after warming they were transferred into recipient females. Subsequently, six viable offspring were delivered, and all were confirmed to be transgenic mice. These results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm.
  • Inhibition of Oct4 expression in mouse preimplantation embryos using morpholino antisense oligonucleotides, Tsuji, I, T Mitani, A Mitsuhashi, Y Watanabe, Y Hosoi, H Hoshiai, TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 208(4), 333 - 342, Apr. 2006 , Refereed
    Summary:Morpholino oligonucleotides (MO) can induce gene silencing by binding to a target mRNA and inhibiting its translation, and this technique has been especially successful in studies of embryonic development in various vertebrates. But in mice MO-induced downregulation of target genes has not been widely reported. In this study, we examined whether MO delivery using ethoxylated polyethylenimine (EPEI) delivery reagent is useful for silencing gene expression in the mouse preimplantation embryo, by targeting endogenous gene Oct4. To optimize the conditions for MO delivery, we examined the MO concentration, the EPEI concentration, the treatment time, and the number of MO treatments. The MO treatment was performed at the 2-cell, the morula, the blastocyst, and the hatched blastocyst stage. We first determined the optimal conditions for MO delivery into the nucleus using fluorescein isothiocianate (FITC)-labeled MO, and demonstrated that treatment with a combination of 20 mu M MO and 0.56 mu M EPEI for 3 hrs produced effective MO delivery. MO-induced downregulation of Oct4 was then examined. Two-step MO treatment at the 2-cell and blastocyst stages successfully suppressed Oct4 expression. This MO treatment resulted in marked reduction of Oct4 protein at the blastocyst stage. After cultivation of blastocysts for further 4 days, derivatives of embryos either differentiated to trophoblastic cells or showed developmental arrest at the blastocyst. This phenocopy is similar to Oct4-deficient embryos. Overall, our results indicate that MO delivery with EPEI is an effective tool for analyzing gene function in mouse preimplantation embryos.
  • Relation of spatial gene expression patterns in bovine embryos reconstructed with somatic cells to blastocyst development, Saeki K, Tamari T, Kasamatsu A, Iwamoto D, Kameyama S, Tatemizo A, Mitani T, Kato H, Hosoi Y, Matsumoto K, Taniguchi S, Ideta A, Urakawa M, Aoyagi Y, Iritani A, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 18(1-2), 142 - 143, 2006 , Refereed
  • Transgenic small interfering RNA halts amyotrophic lateral sclerosis in a mouse model, Y Saito, T Yokota, T Mitani, K Ito, M Anzai, M Miyagishi, K Taira, H Mizusawa, JOURNAL OF BIOLOGICAL CHEMISTRY, JOURNAL OF BIOLOGICAL CHEMISTRY, 280(52), 42826 - 42830, Dec. 2005 , Refereed
    Summary:Many autosomal dominant diseases such as familial amyotrophic lateral sclerosis (ALS) with copper/zinc superoxide dismutase (SOD1) mutation may be induced by missense point mutations that result in the production of proteins with toxic properties. Reduction in the encoding of proteins from such mutated genes can therefore be expected to improve the disease phenotype. The duplex of 21-nucleotide RNA, known as small interfering RNA (siRNA), has recently emerged as a powerful gene silencing tool. We made transgenic (Tg) mice with modified siRNA, which had multiple mismatch alternations within the sense strand, to prevent the "shutdown phenomenon" of transgenic siRNA. Consequently, the in vivo knockdown effect of siRNA on SOD1 expression did not diminish over four generations. When we crossed these anti-SOD1 siRNA Tg mice with SOD1(G93A) Tg mice, a model for ALS, siRNA prevented the development of disease by inhibiting mutant G93A SOD1 production in the central nervous system. Our findings clearly proved the principle that siRNA-mediated gene silencing can stop the development of familial ALS with SOD1 mutation.
  • Development of autoimmunity against transcriptionally unrepressed target antigen in the thymus of aire-deficient mice, N Kuroda, T Mitani, N Takeda, N Ishimaru, R Arakaki, Y Hayashi, Y Bando, K Izumi, T Takahashi, T Nomura, S Sakaguchi, T Ueno, Y Takahama, D Uchida, SJ Sun, F Kajiura, Y Mouri, HW Han, A Matsushima, G Yamada, M Matsumoto, JOURNAL OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, 174(4), 1862 - 1870, Feb. 2005 , Refereed
    Summary:Autoimmune regulator (AIRE) gene mutation is responsible for the development of organ-specific autoimmune disease with monogenic autosomal recessive inheritance. Although Aire has been considered to regulate the elimination of autoreactive T cells through transcriptional control of tissue-specific Ags in thymic epithelial cells, other mechanisms of AIRE-dependent tolerance remain to be investigated. We have established Aire-deficient mice and examined the mechanisms underlying the breakdown of self-tolerance. The production and/or function of immunoregulatory T cells were retained in the Aire-deficient mice. The mice developed Sjogren's syndrome-like pathologic changes in the exocrine organs, and this was associated with autoimmunity against a ubiquitous protein, a-fodrin. Remarkably, transcriptional expression of alpha-fodrin was retained in the Aire-deficient thymus. These results suggest that Aire regulates the survival of autoreactive T cells beyond transcriptional control of self-protein expression in the thymus, at least against this ubiquitous protein. Rather, Aire may regulate the processing and/or presentation of self-proteins so that the maturing T cells can recognize the self-Ags in a form capable of efficiently triggering autoreactive T cells. With the use of inbred Aire-deficient mouse strains, we also demonstrate the presence of some additional factor(s) that determine the target-organ specificity of the autoimmune disease caused by Aire deficiency.
  • Cytological analysis of hepatic gene expression and immunological response of MHC antigens in mouse amniotic epithelial cells, Mitani T, Nagai T, Suzuki D, Ukida Y, Kato H, Matsumoto K, Saeki K, Hosoi Y, Iritani A, REPRODUCTION FERTILITY AND DEVELOPMENT, REPRODUCTION FERTILITY AND DEVELOPMENT, 17(1-2), 208 - 208, 2005 , Refereed
  • Nucling recruits Apaf-1/pro-caspase-9 complex for the induction of stress-induced apoptosis, T Sakai, L Liu, XC Teng, R Mukai-Sakai, H Shimada, R Kaji, T Mitani, M Matsumoto, K Toida, K Ishimura, Y Shishido, TW Mak, K Fukui, JOURNAL OF BIOLOGICAL CHEMISTRY, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(39), 41131 - 41140, Sep. 2004 , Refereed
    Summary:Nucling is a novel protein isolated from murine embryonal carcinoma cells with an up-regulated expression during cardiac muscle differentiation. We show here that Nucling was up-regulated by proapoptotic stimuli and important for the induction of apoptosis after cytotoxic stress. We further demonstrated that overexpressed Nucling was able to induce apoptosis. In Nucling-deficient cells, the expression levels of Apaf-1 and cytochrome c, which are the major components of an apoptosis-promoting complex named apoptosome, were both down-regulated under cellular stress. A deficiency of Nucling also conferred resistance to apoptotic stress on the cell. After UV irradiation, Nucling was shown to reside in an Apaf-1/pro-caspase-9 complex, suggesting that Nucling might be a key molecule for the formation and maintenance of this complex. Nucling induced translocation of Apaf-1 to the nucleus, thereby distributing the Nucling/Apaf-1/pro-caspase-9 complex to the nuclear fraction. These findings suggest that Nucling recruits and transports the apoptosome complex during stress-induced apoptosis.
  • K-ATP channel knockout mice crossbred with transgenic mice expressing a dominant-negative form of human insulin receptor have glucose intolerance but not diabetes, Y Kanezaki, T Obata, R Matsushima, A Minami, T Yuasa, K Kishi, Y Bando, H Uehara, K Izumi, T Mitani, M Matsumoto, Y Takeshita, Y Nakaya, T Matsumoto, Y Ebina, ENDOCRINE JOURNAL, ENDOCRINE JOURNAL, 51(2), 133 - 144, Apr. 2004 , Refereed
    Summary:Impaired insulin secretion and insulin resistance are thought to be two major causes of type 2 diabetes mellitus. There are two kinds of diabetic model mice: one is a K-ATP channel knockout (Kir6.2KO) mouse which is defective in glucose-induced insulin secretion, and the other is a transgenic mouse expressing the tyrosine kinase-deficient (dominant-negative form of) human insulin receptor (hIR(KM)TG), and which has insulin resistance in muscle and fat. However, all of these mice have no evidence of overt diabetes. To determine if the double mutant Kir6.2KO/hIR(KM)TG mice would have diabetes, we generated mutant mice by crossbreeding, which would show both impaired glucose-induced insulin secretion and insulin resistance in muscle and fat. We report here that: 1) blood glucose levels of randomly fed and 6 h fasted double mutant (Kir6.2KO/hIR(KM)TG) mice were comparable with those of wild type mice; 2) in intraperitoneal glucose tolerance test (ipGTT), Kir6.2KO/hIR(KM)TG mice had an impaired glucose tolerance; and 3) during ipGTT, insulin secretion was not induced in either Kir6.2KO/hIR(KM)TG or Kir6.2KO mice, while the hIR(KM)TG mice showed a more prolonged insulin secretion than did wild type mice; 4) hyperinsulinemic euglycemic clamp test revealed that Kir6.2KO, Kir6.2KO/hIR(KM)TG and hIR(KM)TG mice, showed decreased whole-body glucose disposal compared with wild type mice; 5) Kir6.2KO, but not Kir6.2KO/hIR(KM)TG mice had some obesity and hyperleptinemia compared with wild type mice. Thus, the defects in glucose-induced insulin secretion (Kir6.2KO) and an insulin resistance in muscle and fat (hIR(KM)TG) were not sufficient to lead to overt diabetes.
  • NF-kappa B-inducing kinase establishes self-tolerance in a thymic stroma-dependent manner, F Kajiura, S Sun, T Nomura, K Izumi, T Ueno, Y Bando, N Kuroda, HW Han, Y Li, A Matsushima, Y Takahama, S Sakaguchi, T Mitani, M Matsumoto, JOURNAL OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, 172(4), 2067 - 2075, Feb. 2004 , Refereed
    Summary:Physical contact between thymocytes and the thymic stroma is essential for T cell maturation and shapes the T cell repertoire in the periphery. Stromal elements that control these processes still remain elusive. We used a mouse strain with mutant NF-kappaB inducing kinase (NIK) to examine the mechanisms underlying the breakdown of self-tolerance. This NIK-mutant strain manifests autoimmunity and disorganized thymic structure with abnormal expression of Rel proteins in the stroma. Production of immunoregulatory T cells that control autoreactive T cells was impaired in NIK-mutant mice. The autoimmune disease seen in NIK-mutant mice was reproduced in athymic nude mice by grafting embryonic thymus from NIK-mutant mice, and this was rescued by supply of exogenous immunoregulatory T cells. Impaired production of immunoregulatory T cells by thymic stroma without normal NIK was associated with altered expression of peripheral tissue-restricted Ags, suggesting an essential role of NIK in the thymic microenvironment in the establishment of central tolerance.
  • Essential role of NF-kappa B-inducing kinase in T cell activation through the TCR/CD3 pathway, M Matsumoto, T Yamada, SK Yoshinaga, T Boone, T Horan, S Fujita, Y Li, T Mitani, JOURNAL OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, 169(3), 1151 - 1158, Aug. 2002 , Refereed
    Summary:NF-kappaB-inducing kinase (NIK) is involved in lymphoid organogenesis in mice through lymphotoxin-beta receptor signaling. To clarify the roles of NIK in T cell activation through TCR/CD3 and costimulation pathways, we have studied the function of T cells from aly mice, a strain with mutant NIK. NIK mutant T cells showed impaired proliferation and IL-2 production in response to anti-CD3 stimulation, and these effects were caused by impaired NF-kappaB activity in both mature and immature T cells; the impaired NF-kappaB activity in mature T cells was also associated with the failure of maintenance of activated NF-kappaB. In contrast, responses to costimulatory signals were largely retained in aly mice, suggesting that NIK is not uniquely coupled to the costimulatory pathways. When NIK mutant T cells were stimulated in the presence of a protein kinase C (PKC) inhibitor, proliferative responses were abrogated more severely than in control mice, suggesting that both NIK and PKC control T cell activation in a cooperative manner. We also demonstrated that NIK and PKC are involved in distinct NF-kappaB activation pathways downstream of TCR/CD3. These results suggest critical roles for NIK in setting the threshold for T cell activation, and partly account for the immunodeficiency in aly mice.
  • Transplantation of amniotic epithelial cells into fetal rat liver by in utero manipulation, N Takahashi, S Enosawa, T Mitani, H Lu, S Suzuki, H Amemiya, T Amano, N Sakuragawa, CELL TRANSPLANTATION, CELL TRANSPLANTATION, 11(5), 443 - 449, 2002 , Refereed
    Summary:It has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procurement. But transplantation of a sufficient number of cells to adult tissue needs large-scale cell supply and may lead to vascular embolism. We attempted transplantation of amniotic epithelial cells into fetal liver, because 1) the fetal liver is at the proliferative stage, 2) the number of cells required is small, and 3) the fetal stage is advantageous for the induction of immunological tolerance. Amniotic epithelial cells from day 18.5-20.5 fetuses were transfected with adenoviral AdlacZ and harvested to inject into fetal rat liver of the syngeneic strain (day 18.5-20.5). The efficacy of cell transplantation into the liver increased in the order: intraplacental < intraumbilical vein < intrahepatic route. LacZ-transfected amniotic cells (1-8 x 10(5) cells), hepatocytes (5 x 10(5) cells), or AdlacZ vector solution (1.7 x 10(7) pfu) were injected through the uterine membrane into the liver. Transplanted cells formed a cellular mass and survived for up to 14 days after birth, whereas lacZ-transfected cells were rapidly decreased after the injection of AdlacZ vector or rat hepatocytes as a gene carrier so that the use of amniotic epithelial cells as a gene carrier will result in long-term expression of exogenous genes in the liver.
  • Cytological examination of rat amniotic epithelial cells and cell transplantation to the liver, T Nakajima, S Enosawa, T Mitani, XK Li, S Suzuki, H Amemiya, O Koiwai, N Sakuragawa, CELL TRANSPLANTATION, CELL TRANSPLANTATION, 10(4-5), 423 - 427, 2001 , Refereed
    Summary:It is hoped that amniotic epithelial cells can be useful in cell-mediated gene therapy. We report here an experimental cell transplantation model of amniotic cells in rats. There is an anatomical difference between human and rodent embryos. We established a method to isolate amniotic cells that are equivalent to human amniotic epithelial cells. An amniotic membrane distinct from the yolk sac was carefully collected and teased in saline containing deoxyribonuclease and hyaluronidase, followed by collagenase digestion. The cell yield was approximately 10(6) cells per pregnant female (10(5) cells per fetus), roughly in proportion to the age of fetus used, and 60% of the isolated cells were attached to the dish under culture conditions. Telomerase activity was higher in the cells isolated from fetuses in the middle stage (day 13.5 to 15.5) than in the late stage (day 17.5 to 21.5). Adherent cells exhibited two to three times more cell division, resulting in a ninefold increase in the number of cells. Immunohistochemical analysis revealed that approximately half of the adherent cells were albumin positive and formed clusters. The senescent cells survived for 2 months without apparent morphological changes. The adherent cells were able to be stored in liquid nitrogen and had a viability of 70% when thawed. Gene transduction with adenovirus vector was highly effective for rat amniotic cells, Transplantation of lacZ transfected amniotic cells into syngeneic rat liver resulted in the integration of the transplanted cells in the liver structure and the cells survived for at least 30 days.
  • Abnormal immune function of hemopoietic cells from alymphoplasia (aly) mice, a natural strain with mutant NF-kappa B-inducing kinase, T Yamada, T Mitani, K Yorita, D Uchida, A Matsushima, K Iwamasa, S Fujita, M Matsumoto, JOURNAL OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, 165(2), 804 - 812, Jul. 2000 , Refereed
    Summary:Alymphoplasia (aly) mice, a natural strain with a mutant NF-kappa B-inducing kinase (NIK) gene, manifest a unique phenotype; they lack lymph nodes and Peyer's patches, have a disturbed spleen architecture, and exhibit defects in both Ab and cellular immune responses. Although a stromal defect caused by impaired lymphotoxin-beta receptor signaling accounts for their abnormal lymphoid organogenesis, the exact mechanisms underlying the development of immunodeficiency in aly mice are poorly understood. We therefore investigated the contribution of hemopoietic cells with the aly NIK mutation to the development of immunodeficiency. Transfer of aly/aly bone marrow cells into aly/+ mice resulted in poorly developed B cell follicles and lack of support for the development of germinal centers and isotype switching, indicating that the hemopoietic cells of aly mice contain an autonomous defect. However, follicular dendritic cell clusters were maintained in the spleens of these bone marrow chimeras, suggesting that the lack of follicular dendritic cell clusters in aly mice is probably due to the stromal defect, The aly mice larked marginal zone B cells in their spleens, and aly/aly B cells showed an impaired proliferative response after in vitro stimulation. IL-2 production by activated T cells was also impaired. By contrast, the dendritic cells of aly mice exhibited grossly normal development and function. Supporting the concept of an autonomous cell defect, Rel protein expression was altered in aly/aly spleens. Thus, the aly NIK mutation affects hemopoietic cell function in an intrinsic fashion and, together with the stromal defect, may contribute to the development of immunodeficiency in aly mice.
  • DEVELOPMENTAL ABILITY OF ENUCLEATED BOVINE OOCYTES MATURED INVITRO AFTER FUSION WITH SINGLE BLASTOMERES OF 8-CELL EMBRYOS MATURED AND FERTILIZED INVITRO, T MITANI, K UTSUMI, A IRITANI, MOLECULAR REPRODUCTION AND DEVELOPMENT, MOLECULAR REPRODUCTION AND DEVELOPMENT, 34(3), 314 - 322, Mar. 1993 , Refereed
    Summary:Single blastomeres from eight-cell stage bovine embryos matured and fertilized in vitro were electrically fused with enucleated oocytes matured in vitro. In experiment-1, The percentage of these reconstituted embryos developed to the two- to eight-cell stage 48 hr after electrofusion was increased when both the eight-cell embryos and the enucleated oocytes were derived from oocytes cultured with granulosa cells (14% vs. 38%). In experiment 2, the relationship between activation of oocytes and developmental ability of reconstituted embryos was examined. Although both ethanol and electrical stimulation efficiently induced parthenogenetic activation of oocytes matured in vitro for 26-28 hr (ethanol, 89%; electrical stimulation, 73%), the ratio of the second polar body extrusion differed (80% vs. 22%). Ethanol-treated enucleated oocytes, however, were not significantly different from the early-cleavage of the reconstituted embryos 48 hr after electrofusion (nontreated, 38%; treated, 43%). In experiment 3, reconstituted embryos at the two- to eight-cell stage 48 hr after the electrofusion were cocultured with granulosa cells for 6-7 days. Of 69 embryos, one developed to a morula and three developed to blastocysts.

Books etc

  • Application for visualizing chromosome territories and genes within the mouse embryonic cell nucleus by 3D-FISH technique., TANABE Hideyuki, NAKAYA Masataka, MITANI Tasuku, Joint author,   2017 06
  • Methods in Molecular Biology 48, Animal Cell Electroporation and Electrofusion Protocols, Iritani A, Mitani T, Contributor, Nuclear transfer in bovine embryos., Humana Press,   1995 , 089603304X

Conference Activities & Talks

  • Differentiation of arginyltransferase knockout pluripotent stem cells., Minobe K, Kurosaka S, Leu NA, Kashina AS, Mitani T, American Society for Cell Biology Annual Meeting (ASCB2018),,   2018 12 08
  • Involvement of P-TEFb component, cyclin T1 and T2, in the phosphorylation of RNAPII-Ser2 and de novo RNA synthesis in the fertilized egg., Takamatsu S, Hosokawa M, Nakano T, Yamamichi A, Shirouzu S, Kurosaka S, Mitani T, The 41st Annual Meeting of the Molecular Bioloby Society of Japan,   2018 11 28
  • Methylglyoxal, a precursor of advanced glycation end products (AGEs), induces deterioration in oocyte quality in mice., Nakano T, Onishi K, Takamatsu S, Hosokawa M, Kurosaka S, Nakaoka Y, Morimoto Y, Mitani T, The 41st Annual Meeting of the Molecular Biology Society of Japan,   2018 11 28
  • Potential of arginyltransferase 1 (ATE1) knockout pluripotent stem cells to differentiate into cardiomyocytes., Minobe K, Kurosaka S, Mitani T, The 41st Annual Meeting of the Molecular Biology Society of Japan,   2018 11 28
  • Visualization of the chromosomal organization using 3D-FISH in fertilized eggs., Mitani T, The 10th Japan-Korea ART Conference,   2018 08 25 , 招待有り
  • Induction of ABC transporter/Bcrp1 on oxidative stress in mouse ES cells, Hosokawa M, Takamatsu S, Minobe K, Kurosaka S, Taguchi Y, Mitani T, The 40th Annual Meeting of the Molecular Biology Society of Japan,   2017 12 06
  • Visualization of nuclear spatial organization in early mouse embryos; A new “EASI-FISH chamber glass” for use by 3D-FISH technique., Takamatsu S, Nakaya M, Tanabe H, Hosokawa M, Kurosaka S, Mitani T, The 40th Annual Meeting of the Molecular Biology Society of Japan,   2017 12 06
  • Visualization of the spatial arrangement of chromosomal organization using three-dimensional in situ hybridization in early mouse embryos., Mitani T, The 1st International ART Symposium “Current and Future ART”,   2017 10 13 , 招待有り
  • Kinetics of CDK/Cyclin complexes and phosphorylated RNA polymerase II in mouse oocytes and fertilized embryos., Takamatsu S, Nakaya M, Hosokawa M, Shirouzu S, Kurosaka S, Mitani T, The 4th World Congress of Reproductive Biology (WCRB2017),   2017 09 27
  • A new “EASI-FISH chamber glass” for use by 3D-FISH technique: Visualization of nuclear spatial organization in early mouse embryos., Nakaya M, Tanabe H, Takamatsu S, Hosokawa M, Mitani T, 4D-Nucleome Conference,   2017 05 14
  • Dynamics of cyclins and phosphorylation of RNA polymerase II in mouse preimplantation embryos, Nakaya M, Takamatsu S, Hosokawa M, Tanabe H, Mitani T, The 39th Annual Meeting of the Molecular Biology Society of Japan,   2016 11 30
  • Histone H2A.Z: A Janus-faced role in the embryonic development?, Mitani T, Nakaya M, The 8th Japan-Korea ART Conference,   2016 09 03 , 招待有り
  • Dynamic expression of PRMT5 and histone H3 symmetrically dimethylated at arginine 8 in mouse embryos during the 1-cell stage, Tsukaguchi T, Morita K, Higuchi C, Uchibori S, Mitani T, Hosoi Y, Miyamoto K, Matsumoto K, International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming,   2016 03 10
  • Dynamic exprerssion of PRMT5 and histone H3 symmetrically dimethylated at arginine 8 in mouse embryos during the 1-cell stage., Tsukaguchi T, Morita K, Higuchi C, Uchibori S, Mitani T, Hosoi Y, Miyamoto K, Matsumoto K, International Symposium on Epigenetic Dynamics and Regulation in Germ Cells,   2016 02 17
  • Dynamic changes of nucleosome conformation in the mouse somatic cell nuclear transfer embryo by the treatment of HDAC inhibitors., Nakaya M, Nakamae S, Kanba T, Anzai M, Kishigami S, Hosoi Y, Harata M, Mitani T, The International Symposium on Chromatin Structure, Dynamics, and Function,   2015 08 23
  • Dynamic changes of histone chaperones, Tip60 and ANP32E, histone H3 modification and histone H2A.Z withdrawal in the mouse somatic cell nuclear transfer embryos by the treatment of HDAC inhibitors., Nakaya M, Nakamae S, Kanba T, Anzai M, Kishigami S, Hosoi Y, Harata M, Mitani T, 48th Annual Meeting of Society for the Study of Reproduction,   2015 06 19
  • HDAC Inhibitors modulate subcellular localization of histone H2A.Z, acetylated H3 and the histone chaperones, Tip60 and ANP32E, in the mouse somatic cell nuclear transfer embryos., Nakaya M, Kanba T, Nakamae S, Anzai M, Kishigami S, Hosoi Y, Harata M, Mitani T, IFFS/JSRM International Meeting 2015,   2015 04 26
  • The study of density estimation and habitation condition of Tokudaia spp, in Ryukyu Islands, Japan., Jogahara T, Koshimoto C, Sakamoto S, Mitani T, Nakaya M, Kuroiwa A, Yamada F, 14th Rodens et Spatium -International Conference on Rodents Biology-,   2014 08
  • Dynamics of histone H2A variants in the mouse somatic nuclear transfer embryos by the treatment of HDAC inhibitors, Nakaya M, Azuma R, Anzai M, Kishigami S, Hosoi Y, Harata M, Mitani T, 47th Annual Meeting of the Japanese Society of Developmental Biologists, Co-sponsor: Asia-Pacific Developmental Biology Network,   2014 05 27
  • Expression of GSE proteins in primordial germ cells., Morita K, Hatanaka Y, Shimizu N, Nishikawa S, Nishihara T, Kato R, Takemoto A, Higuchi C, Amano T, Kishigami S, Kato H, Mitani T, Hosoi Y, Matsumoto K,   2012 12 12
  • GSE is a maternal factor for DNA demethylation in early embryos., Hatanaka Y, Shimizu N, Morita K, Nishikawa S, Nishihara T, Kato R, Takemoto A, Higuchi C, Amano T, Kishigami S, Anzai M, Kato H, Mitani T, Hosoi Y, Matsumoto K,   2012 12 12
  • Chromatin remodeling that take advantage of histone H2A.Z of mouse embryonic stem cells., Emoto T, Kawaguchi S, Nakaya M, Hirano D, Kishigami S, Hosoi Y, Harata M, Mitani T,   2012 12 12
  • The effect of trichostatin A on the dynamics of histone H2A variants in mouse somatic cell nuclear transfer embryos., Nakaya M, Emoto T, Kawaguchi S, Takatsu H, Anzai M, Hosoi Y, Harata M, Mitani T,   2012 12 12
  • Transcriptional regulation of Bcrp1 mRNA isoform A in mouse ES cells., Hirano D, Honda M, Emoto T, Kawaguchi S, Nakaya M, Fujiwara Y, Yuno M, Taguchi Y, Hosoi Y, Mitani T,   2012 12 12
  • Looping out model of tissue specific gene loci with hepatocyte differentiation process of mouse embryonic stem cells., Kawaguchi S, Emoto T, Hirano D, Nakaya M, Hosoi Y, Tanabe H, Mitani T,   2012 12 12
  • Modulating the Distribution of Histone Variants in the Mouse Somatic Cell Nuclear Transfer Embryos, Mitani T, 4th Congress of Asia Pacific Initiative on Reproduction (ASPIRE2012),   2012 09 01 , 招待有り
  • Transcriptional regulation of Bcrp1 mRNA splice-variants in mouse embryonic stem cells., Hirano D, Kawaguchi S, Honda M, Taguchi Y, Hosoi Y, Mitani T,   2011 12 13
  • Looping out of gene loci of hepatocyte specific gene Tdo2 in chromosome territories under the in vitro differentiation of mouse embryonic stem cells., Kawaguchi S, Emoto T, Hosoi Y, Tanabe H, Mitani T,   2011 12 13
  • Reconstitution of seminiferous tubes by xenoectopic transplantation of bovine testicular cells into the subcutis of immunodeficient mice., Mitani T, Moriki K, Kita S, Taniguchi S, Anzai M, Saeki K, Hosoi Y, Iritani A, 11th International Symposium on Spermatology,   2010 06 24
  • Fibroblast growth factor 4 promotes the development of somatic cell nuclear transfer embryos in mice., Mitani T, Morita M, Anzai M, Nishiyama Y, Moriki K, Kawamura H, Kato H, Saeki K, Hosoi Y, Iritani A, 36th Annual Conference of the International Embryo Transfer Society,   2010 01 09
  • The effect of Dnmt1p mRNA knockdown on Dnmt1 protein translation in mouse testis., Kato K, Kitamura R, Yamaguchi H, Numata Y, Kijima T, Anzai M, Mitani T, Matsumoto K, Saeki K, Hosoi Y, Iritani A, 38th Annual Conference of the International Embryo Transfer Society,   2010 01 07
  • Differentiation of mouse ES cells to hepatocytes and its application., Mitani T, Recent Advance of Embryonic and Somatic Stem Cells in Biomedical Science Workshop,   2008 07 28 , 招待有り
  • Viability and DNA fragmentation of mouse frozen bone marrow cells without cryoprotectant and its availability for nuclear donor in somatic nuclear transfer., Kato H, Nakao A, Nishiwaki M, Anzai M, Mitani T, Iritani A, 16th International Congress of Animal Reproduction,   2008 07 13
  • Expression of the genes implicated in the development of the trophoblast lineage in mouse somatic cell nuclear transfer embryos., Mitani T, Nishiwaki M, Morita M, Moriki K, Nishiyama Y, Kato H, Anzai M, Iritani A, 16th International Congress of Animal Reproduction,   2008 07 13
  • Expression of Bcrp1 mRNA isoforms during in vitro differentiation of mouse embryonic stem cells., Kawamura H, Amimoto N, Moriki K, Morita M, Anzai M, Kato H, Hosoi Y, Iritani A, Mitani T, 41st Annual Meeting for the Japanese Society of Developmental Biologists (jointly sponsored by the International Society of Developmental Biologists),   2008 05 28
  • Expression of stem and germ cell markers in juvenile bovine testis and its primary culture., Moriki K, Tazuhara Y, Taniguchi S, Kawamura H, Anzai M, Kato H, Saeki K, Iritani A, Mitani T, 41st Annual Meeting for the Japanese Society of Developmental Biologists (jointly sponsored by the International Society of Developmental Biologists),   2008 05 28
  • Expression of the genes implicated in the embryonic development during peri-implantation period in mouse somatic cell nuclear transfer embryos., Morita M, Nishiwaki M, Moriki K, Kawamura H, Anzai M, Kato H, Iritani A, Hosoi Y, Mitani T, 41st Annual Meeting for the Japanese Society of Developmental Biologists (jointly sponsored by the International Society of Developmental Biologists),   2008 05 28
  • siRNA transgenic mice as a tool for studying neurological diseases, Sasaguri H, Yokota T, Saito Y, Anzai M, Mitani T, Mizusawa H, 34th Annual Meeting of Society for Neuroscience,   2007 11 07
  • In vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells using monolayer-culture system., Mitani T, Nagai T, Masutani T, Kato H, Matsumoto K, Saeki K, Hosoi Y, Iritani A, 3rd Annual Conference of Asian Reproductive Biotechnology Society,   2006 11 29
  • Expression of maternal gene promoter driven expression vectors in mouse oocytes and fertilized eggs., Tsunemoto K, Matsumoto K, Hayakumo M, Endo N, Anzai M, Amano T, Mitani T, Kato H, Hosoi Y, Saeki K, Iritani A, 20th IUBMB Int’l. Congr. Biochem. Mol. Biol. and 11th FAOBMB Congr.,   2006 06 18
  • Production of C57BL/6 embryonic stem cell-derived mice using methods of ICR tetraploid aggregation and 4-cell or blastocyst injection., Kawata N, Yamochi T, Anzai M, Mitani T, Saeki K, Matsumoto K, Hosoi Y, Iritani A, The 17th Annual and International Meeting of the Japanese Association for Animal Cell Technology,   2004 10
  • Toxicological studies employing the ES cell lines derived from C57BL/6 mice., Kasai S, Yamochi T, Mitani T, Saeki K, Matsumoto K, Hosoi Y, Iritani A, The 17th Annual and International Meeting of the Japanese Association for Animal Cell Technology,   2004 10
  • Kinetics of destabilized luc+ gene expression in mouse preimplantation embryos., Matsumoto K, Hitomi S, Ikeda M, Tsuzaki M, Anzai M, Hosoi Y, Saeki K, Mitani T, Kato H, Iritani A, 4th Conference of the Pacific Rim Society for Fertility and sterility,   2004 03 08
  • Establishment of ES cell lines from diploid androgenetic embryo for producing germline chimera., Kato H, Murakami H, Kawasumi M, Kunieda T, Anzai M, Mitani T, Matsumoto K, Saeki K, Hosoi Y, Iritani A, 4th Conference of the Pacific Rim Society for Fertility and sterility,   2004 03 08
  • Effects of cell cycle control methods for donor somatic cells on gene expression and in vitro development of bovine reconstituted embryos., Saeki K, Kasamatsu A, Tamari T, Shirouzu K, Taniguchi S, Mitani T, Matsumoto K, Hosoi Y, Iritani A, 4th Conference of the Pacific Rim Society for Fertility and sterility,   2004 03 08
  • Gene and cell transplantation to the rat placenta by in-utero manipulation., Mitani T, Enosawa S, 4th Conference of the Pacific Rim Society for Fertility and sterility,   2004 03 08
  • Transplantation of amniotic epithelial cells to fetal rat liver by in-utero manipulation with an aim of fetal therapy., Enosawa S, Lu H, Takahashi N, Mitani T, Suzuki S, Amemiya H, Amano T, Sakuragawa N, China-Japan Medical Conference 2002,   2002 11
  • A novel cell population which shows hepatocyte-like differentiation markers in human amniotic epitherial cells. –A possible gene carrier for cell therapy-., Enosawa S, Sakuragawa N, Mitani T, Li X-K, Tamura A, Suzuki S, Amemiya H, The Cell Transplant Society, Fourth International Congress,   1999 03
  • Transcriptional activity and morphological changes of somatic cell nuclei transferred into germinal vesicle stage oocytes., Okumura I, Minobe K, Mitani T, Kurosaka S, 2019 ASCB | EMBO Meeting,   2019 12 10

Misc

  • 初期胚特異的レトロトランスポゾンMERVLの活性化機構の解析, 奥野智美, 樋口智香, 神谷拓磨, 山本真理, 越智浩介, 西野亜理紗, 井橋俊哉, 辻本佳加理, 松橋珠子, 安齋政幸, 黒坂哲, 三谷匡, 山縣一夫, 細井美彦, 松本和也, 宮本圭, Journal of Reproduction and Development, 64, Suppl Japanese Issue, j125, 70-P-70,   2018 09 05 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802285393389434
    Summary:<p>【目的】哺乳類ゲノムの約半分は,トランスポゾン由来の配列で構成されている。特に,Murine Endogenous RetroVirus-L(MERVL)と呼ばれるレトロトランスポゾンは,マウス2細胞期胚にかけてその発現が一過的に上昇する独特な発現様式を示し,2細胞期胚特異的に発現する遺伝子群の転写活性を制御している。また,マウスES細胞においてもMERVLを発現する細胞群が存在する。しかし,MERVLの活性化機構の全容は未だ明らかにされていない。そこで本研究では,マウスES細胞を用いて,MERVLの遺伝子発現調節領域(LTR領域)の活性化が誘導される条件を検討した。【方法】マウスES細胞E14Tg2a株を用い,ヒストン脱アセチル化酵素阻害剤であるTrichostatin A(TSA)及びクロマチンのメチル化状態を変化させるVitamin C(VC)を培地に添加した。まず,10 nM TSA添加区,10 ng/µl VC添加区,10 nM TSA及び10 ng/µl VC添加区において,RT-qPCRを用いて2細胞期胚で強発現する遺伝子の転写量を比較した。さらに,2C::td Tomatoベクターをゲノム上に組み込み,MERVL-LTR領域の活性化を可視化できるES細胞を用いて,各培養条件下におけるMERVL-LTR領域活性化細胞数の割合を調べた。【結果】7日間TSAを添加した培養条件下において,2細胞期胚特異的遺伝子群の転写量が有意に上昇していた。また,TSA処理継続時間に比例してMERVL-LTR領域活性化細胞数の割合が約5%まで増加することがわかった。以上の結果より,ES細胞においてMERVL-LTR領域を活性化する培養条件を示した。また,TSA処理によるMERVL活性化促進の結果より,ゲノムワイドなクロマチン弛緩がMERVL-LTR領域の活性化につながる可能性が示唆された。現在,クロマチン脱凝縮を誘導する因子の過剰発現がMERVL活性化を促すか検討を進めている。</p>
  • レトロトランスポゾンMERVLの活性化機構の解析, 奥野智美, 山口壮輝, 樋口智香, 神谷拓磨, 山本真理, 越智浩介, 西野亜理紗, 井橋俊哉, 辻本佳加理, 坂本裕子, 松橋珠子, 安齋政幸, 黒坂哲, 三谷匡, 山縣一夫, 細井美彦, 松本和也, 宮本圭, 日本分子生物学会年会プログラム・要旨集(Web), 41st, ROMBUNNO.2P‐0413 (WEB ONLY),   2018 , https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201802273880748976
  • Application for visualizing chromosome territories and genes within the mouse embryonic cell nucleus by 3D-FISH technique, Tanabe H, Nakaya M, Mitani T, Seitai no Kagaku, 68, 3, 237, 242,   2017 06 , 招待有り, 10.11477/mf.2425200619
  • マウスGV期由来卵子を用いた体外成熟後の卵子母性制御機構に関する基礎検討, SAKITA MEGUMI, KAMEI MIKU, NAKAGAWA TAKAO, NISHIMURA MANAMI, NAKAIE MASATAKA, KOBAYASHI SHINTARO, HIGASHI RIKA, MITANI TASUKU, KATO HIROMI, KISHI MASAO, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 日本実験動物学会総会講演要旨集, 61st, 245,   2014 05 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402283057517607
  • 体外成熟培地へのアミノ酸誘導体添加がマウス未成熟卵子由来初期胚の発生に与える効果, KAMEI MIKU, SAKITA MEGUMI, NAKAGAWA TAKAO, NAKAIE MASATAKA, NISHIMURA MANAMI, HIGASHI RIKA, KOBAYASHI SHINTARO, MITANI TASUKU, KATO HIROMI, KISHI MASAO, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 日本実験動物学会総会講演要旨集, 61st, 246,   2014 05 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402287287945670
  • マウス始原生殖細胞におけるGSEタンパク質の発現解析, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIHARA TAKUSHI, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, UCHIHORI SHO, AMANO TOMOKO, NAGAI KOHEI, KISHIGAMI SATOSHI, KATO HIROKI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, J Reprod Dev, 59, Suppl Japanese Issue, J114,   2013 08 20 , 10.14882/jrds.106.0.P-26.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302273473633087
  • 近交系マウス由来未成熟卵子を用いた生殖補助技術により作出した各卵子母性制御機構に関する検討, ANZAI MASAYUKI, NISHIMURA MANAMI, AZUMA RIKA, NAKAIE MASATAKA, KAMEI MIKU, SAKITA MEGUMI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 1P-0669 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402206788995589
  • マウスES細胞においてヒストンH2A.Zはヒストン脱アセチル化酵素阻害剤により選択的に除去される, KEIMOTO TETSUYA, NAKAIE MASATAKA, KATO HIROMI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, HARADA MASAHIKO, MITANI TASUKU, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 1P-0328 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402295249672230
  • マウス始原生殖細胞で発現するGSEタンパク質の能動的DNA脱メチル化への関与, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIHARA TAKUSHI, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, UCHIBORI SHO, AMANO TOMOKO, NAGAI KOHEI, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 2P-0252 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402296885395420
  • マウスGSEの始原生殖細胞における発現プロファイルは,性差がある, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIKAWA SATOSHI, NISHIHARA TAKUJI, KATO RIE, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, AMANO TOMOKO, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, 日本分子生物学会年会プログラム・要旨集(Web), 35th, 1P-0126 (WEB ONLY),   2012 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302220460117423
  • Recent research on Tokudaia, Mammalian Science, 51, 1, 154, 158,   2011
  • 卵子活性化に伴うチューブリンのアセチル化の解析, 松原 圭吾, Lee Ah Reum, 鎌田 悠, 孫谷 匡輝, 奥山 紀之, 三谷 匡, 加藤 博己, 安齋 政幸, 佐伯 和弘, 松本 和也, 入谷 明, 岸上 哲士, 細井 美彦, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 83回・33回, 1P, 0889,   2010 12
  • 卵子および一細胞期胚におけるタンパク質のアセチル化に関する解析, 松原 圭吾, Lee Ah Reum, 鎌田 悠, 孫谷 匡輝, 三谷 匡, 加藤 博己, 安齋 正幸, 佐伯 和弘, 松本 和也, 入谷 明, 岸上 哲士, 細井 美彦, The Journal of Reproduction and Development, 56, Suppl., j64, j64,   2010 08 , 10.14882/jrds.103.0.12.0
  • マウス初期胚における発生制御タンパク質の解析, 野老 美紀子, 川澄 みゆり, 永井 宏平, 池上 春香, 申 承旭, 西川 慧, 畑中 勇輝, 西原 卓志, 天野 朋子, 三谷 匡, 加藤 博巳, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 56, Suppl., j120, j120,   2010 08 , 10.14882/jrds.103.0.124.0
  • Influence of insemination concentration when in vitro fertilization after zona drilled by laser with C57BL/6 in vitro maturation oocytes, NISHIMURA Manami, NISHIYAMA Yui, YANAGI Miho, KAWABE Toshiaki, MITANI Tasuku, HOSOI Yoshihiko, ANZAI Masayuki, 27, 2,   2010 04 , http://ci.nii.ac.jp/naid/10026350802
  • マウス胚性幹細胞におけるABCトランスポーター・Bcrp1mRNAアイソフォームAの転写制御領域の解析, HIRANO DAIKI, KAWAMURA HIROKO, NISHIMURA YUI, SAEKI KEITA, ANZAI MASAYUKI, KATO HIROMI, TAGUCHI YOSHITOMO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, ROMBUNNO.4P-0843,   2010 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002274122783214
  • マウス初期胚におけるDD2‐2遺伝子は2OSプロテアソームの形成に関与している, SHIN SHOKYOKU, TOKORO MIKIKO, NISHIKAWA KEI, RI KOKIN, HATANAKA YUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 日本畜産学会大会講演要旨, 111th, 78,   2009 09 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223084547469
  • マウスの着床前胚におけるユビキチンプロテアソーム経路による母体蛋白質変性の解析(Analysis of degraded maternal proteins by ubiquitin-proteasome pathway in mouse preimplantation embryo), 李 香欣, 申 承旭, 野老 美紀子, 西川 慧, 畑中 勇輝, 天野 朋子, 岸上 哲士, 安齋 政幸, 三谷 匡, 加藤 博己, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, 日本畜産学会大会講演要旨集, 111回, 78, 78,   2009 09
  • マウス初期胚におけるDD2-2遺伝子は20Sプロテアソームの形成に関与している, 申 承旭, 野老 美紀子, 西川 慧, 李 香欣, 畑中 勇輝, 天野 朋子, 三谷 匡, 加藤 博己, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, 日本畜産学会大会講演要旨集, 111回, 78, 78,   2009 09
  • プロテオミクスによるマウス着床前期胚の発生関連タンパク質の解析, 野老 美紀子, 川澄 みゆり, 永井 宏平, 池上 春香, 申 承旭, 西川 慧, 李 香欣, 畑中 勇輝, 天野 朋子, 三谷 匡, 加藤 博己, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, 日本畜産学会大会講演要旨集, 111回, 78, 78,   2009 09
  • FGF4がマウス体細胞核移植胚の発生に与える効果, MORITA MASAHIRO, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, MITANI TAKUMI, IRIYA AKIRA, J Reprod Dev, 55, Supplement, J84,   2009 08 25 , 10.14882/jrds.102.0.134.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902229379782638
  • マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響, KAWAMURA HIROKO, KAWAI TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROKI, HOSOI YOSHIHIKO, MITANI TADASHI, IRIYA AKIRA, J Reprod Dev, 55, Supplement, J79,   2009 08 25 , 10.14882/jrds.102.0.123.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902291185683946
  • マウス初期胚におけるDD2-2遺伝子は20Sプロテアソームの形成に関与している, 申 承旭, 野老 美紀子, 西川 慧, 李 香欣, 畑中 勇輝, 天野 朋子, 三谷 匡, 加藤 博己, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 善彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 55, Suppl., j74, j74,   2009 08
  • Sr2+が誘導するマウス卵子活性化へのカルシウムキレート剤の利用, 辻本 賀子, 岸上 哲士, 竹原 俊幸, 天野 朋子, 安齋 政幸, 加藤 博巳, 三谷 匡, 松本 和也, 佐伯 和弘, 入谷 明, 細井 美彦, The Journal of Reproduction and Development, 55, Suppl., j84, j84,   2009 08 , 10.14882/jrds.102.0.133.0
  • 若齢ウシ精巣細胞を用いたヌードマウス皮下への異種異所移植による精子形成誘導の試み, 森木 甲子郎, 喜多 章多, 藤本 佑希, 谷口 俊仁, 安齋 政幸, 加藤 博己, 松本 和也, 佐伯 和弘, 細井 美彦, 三谷 匡, 入谷 明, The Journal of Reproduction and Development, 55, Suppl., j96, j96,   2009 08 , 10.14882/jrds.102.0.220.0
  • 胎子及び幼若マウスにおける生殖腺特異的発現遺伝子GSEの発現解析, 畑中 勇輝, 佐藤 学, 野老 美紀子, 申 承旭, 西川 慧, 李 香欣, 天野 朋子, 三谷 匡, 加藤 博己, 岸上 哲士, 細井 美彦, 佐伯 和弘, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 55, Suppl., j120, j120,   2009 08 , 10.14882/jrds.102.0.1030.0
  • プロテオミクスを用いたマウス初期胚における発生関連タンパク質の解析, 野老 美紀子, 川澄 みゆり, 永井 宏平, 池上 春香, 申 承旭, 西川 慧, 李 香欣, 畑中 勇輝, 天野 朋子, 三谷 匡, 加藤 博己, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 55, Suppl., j142, j142,   2009 08 , 10.14882/jrds.102.0.1075.0
  • マウス初期胚でのユビキチン-プロテアソーム系による母性タンパク質の分解に関する研究, 李 香欣, 申 承旭, 野老 美紀子, 西川 慧, 畑中 勇輝, 天野 朋子, 岸上 哲士, 安齋 政幸, 三谷 匡, 加藤 博己, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 55, Suppl., j143, j143,   2009 08
  • Proteome analysis of mouse preimplantation embryos, TOKORO Mikiko, KAWASUMI Miyuri, NAGAI Kohei, IKEGAMI Haruka, SATOH Manabu, SHIN Seung-Wook, NISHIKAWA Satoshi, HATANAKA Yuki, AMANO Tomoko, MITANI Tasuku, KATO Hiromi, ANZAI Masayuki, KISHIGAMI Satoshi, SAEKI Kazuhiro, HOSOI Yoshihiko, IRITANI Akira, MATSUMOTO Kazuya, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 26, 2,   2009 04 01 , http://ci.nii.ac.jp/naid/10024965056
  • ウシ栄養膜細胞によるリクローン胚の初期発生および胚移植, TANIGUCHI SHUNJI, FUKUHARA JUNKO, KISHI MASAO, IWAMOTO DAISAKU, MATSUI TAKANORI, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 31, 1, 58, 58,   2009 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902299849608913
  • マウスES細胞の分化過程における未分化維持機構においてABCトランスポーターBcrp1の影響, KAWAMURA HIROKO, KAWAI TOMOKO, MORIKI KOSHIRO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 191,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002212646226678
  • マウスES細胞の分化過程におけるArpファミリーならびにクロマチンリモデリング複合体構成因子の動態, MORIKI KOSHIRO, NISHIYAMA YUI, KAWAMURA HIROKO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, HARATA MASAHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 190,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002222527720821
  • FGF4はマウス体細胞核移植胚の初期発生を促進する, MORITA MASAHIRO, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 179,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002238117619833
  • トランスジェニックマウスを用いた母性遺伝子プロモーター解析, 畑中 勇輝, 中野 彰大, 常本 和伸, 安齋 政幸, 佐藤 学, 野老 美紀子, 申 承旭, 渡辺 達也, 清水 なつみ, 天野 朋子, 三谷 匡, 加藤 博己, 岸上 哲士, 細井 美彦, 佐伯 和弘, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 54, Suppl., j75, j75,   2008 08 , 10.14882/jrds.101.0.213.0
  • マウス初期胚でDD2-2遺伝子は20Sプロテアソームの形成に関与している, 申 承旭, 野老 美紀子, 佐藤 学, 西川 慧, 天野 朋子, 岸上 哲士, 安齋 政幸, 三谷 匡, 加藤 博己, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 54, Suppl., j76, j76,   2008 08
  • マウス着床前期胚における大規模プロテオーム解析, 野老 美紀子, 川澄 みゆり, 永井 宏平, 池上 春香, 佐藤 学, 申 承旭, 西川 慧, 清水 なつみ, 天野 朋子, 三谷 匡, 加藤 博己, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 54, Suppl., j95, j95,   2008 08 , 10.14882/jrds.101.0.507.0
  • Promoter activity of three maternal-effect genes in transgenic mice, NAKANO Akihiro, TSUNEMOTO Kazunobu, ANZAI Masayuki, SATO Manabu, TOKORO Mikiko, SHIN Seungwook, HATANAKA Yuki, AMANO Tomoko, MITANI Tasuku, KATO Hiromi, HOSOI Yoshihiko, SAEKI Kazuhiro, IRITANI Akira, MATSUMOTO Kazuya, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 25, 2, S94, S94,   2008 04 01 , http://ci.nii.ac.jp/naid/10021218252
  • In vitroにおける単為発生/雄性発生胚由来ES細胞からの三胚葉由来機能細胞の獲得, ONODERA YUTA, TERAMURA TAKESHI, TAKEHARA TOSHIYUKI, FUKUNAGA NAOTO, ITO SHUNSUKE, MURAKAMI HIDEKI, ANZAI MASAYUKI, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, KISHIGAMI TETSUJI, IRITANI AKIRA, SAGAWA NORIMASA, HOSOI YOSHIHIKO, 再生医療, 7, 242,   2008 02 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902221602095710
  • 雌性単為発生胚と雄性単為発生胚由来ES細胞から誘導したインスリン産生細胞の機能性の検討, TAKEUCHI HIROKI, TERAMURA TAKESHI, KISHIGAMI TETSUJI, ANZAI MASAYUKI, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, SAGAWA NORIMASA, HOSOI YOSHIHIKO, 再生医療, 7, 241,   2008 02 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902242585690223
  • 培養ウシ栄養膜細胞の効率的な単離および核移植, TANIGUCHI TOSHIHITO, IWAMOTO TAISAKU, MATSUI TAKANORI, ABE YUKI, KISHIGAMI SATOSHI, KISHI MASAO, KATO HIROKI, MITANI TADASHI, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 30, 1, 57, 57,   2008 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902250660987191
  • マウス着床前期胚における大規模プロテオーム解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SATO MANABU, SHIN SEUNG-WOOK, NISHIKAWA SATOSHI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 4P-0894,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223409224866
  • 若齢ウシ雄性生殖細胞の同定ならびにヌードマウス皮下移植による精子形成誘導の試み, MORIKI KOSHIRO, KITA SHOTA, TANIGUCHI SHUNJI, ANZAI MASAYUKI, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 3P-0886,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902227490032416
  • トリコスタチンA処理によるマウス体細胞核移植胚の着床初期における胚発生に関わる遺伝子発現の解析, MORITA MASAHIRO, NISHIWAKI MEGUMI, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 4P-0895,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902237521150191
  • マウス初期胚で性腺特異的遺伝子DD2‐2が20Sプロテアソームの形成に及ぼす影響, SHIN SEUNG-WOOK, TOKORO MIKIKO, SATO MANABU, NISHIKAWA SATOSHI, AMANO TOMOKO, KISHIGAMI SATOSHI, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 4P-0892,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902285509934742
  • マウスES細胞分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式およびRNAiによるアイソフォームAノックダウンES細胞株樹立の試み, KAWAMURA HIROKO, AMIMOTO NAOKI, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 1P-1076,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902288909820001
  • 過排卵処理したマウス卵巣におけるプロテオーム解析, 松本 和也, 池上 春香, 永井 宏平, 園 陽平, 野老 美紀子, 申 承旭, 松岡 俊樹, 三谷 匡, 加藤 博己, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 日本胚移植学雑誌, 30, 1, 55, 55,   2008 01
  • マウス排卵卵子における網羅的タンパク質発現(プロテオーム)解析, 野老 美紀子, 川澄 みゆり, 永井 宏平, 池上 春香, 申 承旭, 松岡 俊樹, 佐藤 学, 天野 朋子, 三谷 匡, 加藤 博巳, 安齋 政幸, 岸上 哲士, 佐伯 和弘, 細井 美彦, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 53, Suppl., j137, j137,   2007 09 , 10.14882/jrds.100.0.12047.0
  • Rhophilin-2遺伝子はマウス受精卵における第1分裂の細胞質分裂に関与している, 松岡 俊樹, 野老 美紀子, 申 承旭, 天野 朋子, 三谷 匡, 加藤 博己, 安斎 政幸, 岸上 哲士, 細井 美彦, 佐伯 和弘, 入谷 明, 松本 和也, The Journal of Reproduction and Development, 53, Suppl., j138, j138,   2007 09 , 10.14882/jrds.100.0.12048.0
  • マウス体細胞核移植胚における転写因子Cdx2とOct3/4の発現(Expression of transcription factor Cdx2 and Oct3/4 in mouse somatic cell nuclear transfer embryos), 西脇 恵, 三谷 匡, 安齋 政幸, 加藤 博己, 松本 和也, 佐伯 和弘, 細井 美彦, 入谷 明, 日本発生生物学会・日本細胞生物学会合同大会要旨集, 40回・59回, 81, 81,   2007 05
  • マウス受精卵における分裂機構へのrhophilin‐2 遺伝子の関与, MATSUOKA TOSHIKI, TOKORO MIKIKO, SHIN SERNG-WOOK, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 1P-0457,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902212124018136
  • トランスジェニックマウス(Tgマウス)由来卵子及び着床前胚における母性遺伝子のプロモーター活性, ENDO NORIMASA, TSUNEMOTO KAZUNOBU, ANZAI MASAYUKI, MATSUOKA TOSHIKI, TOKORO MIKIKO, SHIN SEUNGWOOK, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 2P-1309,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902252743397607
  • マウスMII期卵母細胞における網羅的タンパク質発現(プロテオーム)解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SHIN SEUNG-WOOK, MATSUOKA TOSHIKI, SATO MANABU, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 2P-1307,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902268338533254
  • マウス体細胞核移植胚における栄養外胚葉分化に関する転写因子の発現, NISHIWAKI MEGUMI, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 1P-0991,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902297357245626
  • Sperm chromatin structure assay法によるウシ精子DNA断片化の解析, 甲田 俊夫, 岸本 学, 能登 健次, 三谷 匡, 松本 和也, 佐伯 和弘, 細井 美彦, 加藤 博己, 入谷 明, 日本胚移植学雑誌, 29, 1, 62, 62,   2007 01
  • 単為発生胚由来ES細胞からの機能細胞分化誘導, 小野寺 勇太, 寺村 岳士, 掛川 亮, 武内 大輝, 三谷 匡, 松本 和也, 佐伯 和弘, 佐川 典正, 細井 美彦, 入谷 明, 日本生殖医学会雑誌, 51, 4, 294, 294,   2006 10
  • マウス体細胞核移植胚および体外受精胚におけるOct-3/4遺伝子転写調節領域のメチル化, 川澄 みゆり, 海野 祐一, 西脇 恵, 松本 和也, 安齋 政幸, 三谷 匡, 加藤 博己, 天野 朋子, 佐伯 和弘, 細井 美彦, 入谷 明, The Journal of Reproduction and Development, 52, Suppl., j75, j75,   2006 08 , 10.14882/jrds.99.0.62.0
  • 母性発現遺伝子(Histone H1oo(H1oo),Nucleoplasmin2(Npm2),Zygote arrest1(Zar1))のプロモーター領域の解析, 常本 和伸, 松本 和也, 安斎 政幸, 天野 朋子, 三谷 匡, 加藤 博己, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 52, Suppl., j88, j88,   2006 08 , 10.14882/jrds.99.0.87.0
  • 初期G1期細胞によるウシ再構築はいの遺伝子発現状態と初期発生との関係, IWAMOTO TASAKU, SAEKI KAZUHIRO, KASAMATSU REI, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, KATO HIROKI, TANIGUCHI TOSHIHITO, DETA ATSUSHI, URAKAWA MAMI, AOYAGI TAKAHITO, IRIYA AKIRA, 日本畜産学会大会講演要旨, 106th, 84,   2006 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902227977066855
  • ウシゲノム中に存在するレトロトランスポゾンのLTRに関する研究, 岸本 学, 加藤 博己, 三谷 匡, 松本 和也, 佐伯 和弘, 細井 美彦, 入谷 明, 日本畜産学会大会講演要旨集, 106回, 68, 68,   2006 03
  • Localization of the autoimmune regulator (aire) protein in the gonads and embryonic stem cells in mice., 増田 淳大, 三谷 匡, 加藤 博己, 松本 和也, 佐伯 和弘, 細井 美彦, 入谷 明, Memoirs of Institute of Advanced Technology, Kinki University, 11, 15, 21,   2006 03 , http://ci.nii.ac.jp/naid/120005736408
    Summary:近年、多腺性自己免疫性内分泌不全症Ⅰ型 (APECED) の原因遺伝子 aire のノックアウトマウス解析において、卵胞が欠損することが報告された。このことから、生殖巣においてAIRE が何らかの働きをしている可能性が考えられる。しかしながら、AIRE の発現は非常に微量であり、胸腺や脾臓以外の発現部位に関する報告は一致していない。そこで、本研究では、免疫蛍光抗体染色により、生殖巣及び胸腺におけるAIRE 発現及びその局在を組織学的に検討した。その結果、生後1週齢のマウス胸腺及び生後8週齢のマウス卵胞からAIRE タンパク質が検出された。これらの組織においては、AIRE タンパク質が細胞核内でNuclear dots(核内において形成される点状構造)を形成していることが確認され、マウス卵巣においてAIRE が何らかの働きをしていることが示唆された。なお、マウス胚盤胞期胚の内部細胞塊においてAIRE発現がみられたという報告があることから、ES 細胞におけるAIRE 発現の有無についても検討したところ、AIRE タンパクが検出され、細胞核内において形成されるNuclear dots も観察された。このことから、ES細胞においてもAIRE が何らかの機能を果たしている可能性があると考えられる。
  • ウシ栄養膜細胞由来核移植胚作製のための体外受精胚のバイオプシー手法の検討, TANIGUCHI TOSHIHITO, KAMEYAMA SHINJI, YAMAMOTO MASAKO, HAYASHI NOBORU, TACHIMIZO ATSUHIRO, KASAMATSU REI, IWAMOTO TAISAKU, SAEKI KAZUHIRO, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本はい移植学雑誌, 29, 1, 61,   2006 01 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902260329113063
  • ウシ体外受精胚盤胞期胚由来栄養膜細胞を用いた核移植, 亀山 信二, 佐伯 和弘, 林 登, 笠松 礼, 立溝 篤宏, 岩本 太作, 加藤 博己, 三谷 匡, 小林 直彦, 坂口 慎一, 松本 和也, 細井 美彦, 大谷 健, 入谷 明, 日本胚移植学雑誌, 28, 1, 56, 56,   2006 01
  • ホウレンソウ由来Δ12脂肪酸不飽和化酵素遺伝子導入マウスにおけるプロテオーム解析, SHINKAI YUSUKE, MATSUMOTO KAZUYA, AMANO TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, SUZUKI IWANE, MURATA NORIO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 540,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902213559909810
  • マウス初期はい特異的発現ベクターの開発, TSUNEMOTO KAZUNOBU, MATSUMOTO KAZUYA, ANZAI MASAYUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902216149803763
  • マウス初期はいにおけるrhophilin‐2遺伝子の機能解析, MATSUOKA TOSHIKI, HAYAKUMO MASANORI, SONO YOHEI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902246956838577
  • マウス体細胞核移植はいにおけるOct‐4遺伝子転写調節領域のCpGメチル化, UNNO YUICHI, KAWASUMI MIYURI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902268610686626
  • G0期および初期G1期の異なる細胞周期のドナー細胞によるウシ再構築はいの遺伝子発現状態と初期発生との関係, KASAMATSU AYA, SAEKI KAZUHIRO, IWAMOTO DAISAKU, KAMEYAMA SHINJI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, TANIGUCHI SHUNJI, IDETA ATSUSHI, URAKAWA MASAMI, AOYAGI YOSHITO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902258290637987
  • RNA interference as a tool for producing knockdown mice., Mitani T, Yokota T, J. Mamm. Ova Res., 22, 3, 139, 151,   2005 10 , 招待有り, 10.1274/jmor.22.139
  • マウス尾部細胞,ES細胞,IVFはい及びNTはいにおけるOct‐3/4遺伝子転写調節領域のCpGメチル化, UMINO YUICHI, KAWASUMI MIYURI, MATSUMOTO KAZUYA, ANZAI MASAYUKI, AMANO TOMOKO, MITANI MASASHI, KATO HIROKI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, J Reprod Dev, 51, Supplement, J65,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902259427986069
  • ルシフェラーゼ遺伝子導入細胞によるウシ再構築はいの割球における遺伝子発現とその後の初期発生との関係, IWAMOTO TAISAKU, SAEKI KAZUHIRO, KASAMATSU REI, TAMARI TOMOHIRO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, KATO HIROKI, TANIGUCHI TOSHIHITO, IRITANI AKIRA, 日本畜産学会大会講演要旨, 105th, 74,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223942369086
  • ドナー細胞の細胞周期の違いがウシ再構築はいの遺伝子発現状態と初期発生に及ぼす影響, KASAMATSU REI, SAEKI KAZUHIRO, IWAMOTO TAISAKU, KAMEYAMA SHINJI, TAMASATO TOMOHIRO, TACHIMIZO ATSUHIRO, MITANI MASASHI, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, TANIGUCHI TOSHIHITO, IDETA ATSUSHI, URAKAWA MAMI, AOYAGI NORIHITO, IRIYA AKIRA, J Reprod Dev, 51, Supplement, J64,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902291966463623
  • マウス初期胚で発現するrhophilin-2遺伝子の機能解析に関する研究, 松岡 俊樹, 園 洋平, 松本 和也, 天野 朋子, 安斎 政幸, 三谷 匡, 加藤 博己, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 51, Suppl., j57, j57,   2005 08
  • マウス尾部細胞,ES細胞,IVF胚及びNT胚におけるOct-3/4遺伝子転写調節領域のCpGメチル化, 海野 佑一, 川澄 みゆり, 松本 和也, 安斎 政幸, 天野 朋子, 三谷 匡, 加藤 博己, 佐伯 和弘, 細井 美彦, 入谷 明, The Journal of Reproduction and Development, 51, Suppl., j65, j65,   2005 08
  • マウス羊膜上皮細胞における肝特異的遺伝子発現ならびに主要組織適合性抗原の免疫学的応答に関する細胞生物学的解析, 永井 匡, 三谷 匡, 鈴木 大介, 有喜多 康夫, 松本 和也, 佐伯 和弘, 細井 美彦, 入谷 明, 日本発生生物学会大会講演要旨集, 38回, 236, 236,   2005 05
  • ルシフェラーゼ遺伝子導入細胞によるウシ再構築はいでの遺伝子発現とその後の初期発生との関係, TAMARI TOMOHIRO, SAEKI KAZUHIRO, KASAMATSU REI, SHIROZU KOICHIRO, TERAI DAISUKE, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, TANIGUCHI SHUNJI, 日本畜産学会大会講演要旨, 104th, 137,   2005 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902279894360158
  • カニクイザル-ウサギ異種間核移植胚の特性解析, 矢持 隆之, 川田 延幸, 大田 聖, 竹之下 誠, 細井 美彦, 加藤 博己, 三谷 匡, 松本 和也, 佐伯 和弘, 入谷 明, 日本胚移植学雑誌, 27, 1, 45, 45,   2005 01
  • Basic knowledge "Genetically modified mouse (Transgenic mouse, Knockout mouse)", MITANI Tasuku, Nikkei Biotechnology & Business, 2005, 01, 96, 100,   2004 12 , 招待有り
  • 植物由来Δ12脂肪酸不飽和化酵素遺伝子導入マウスにおける網羅的遺伝子発現解析, SHINKAI YUSUKE, MATSUMOTO KAZUYA, AMANO TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SUZUKI IWANE, MURATA NORIO, 日本分子生物学会年会プログラム・講演要旨集, 27th, 1009,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902205325166641
  • マウス初期はいにおけるはい性遺伝子発現機構へのDNAメチル化及びヒストンアセチル化の関与, YAMAMOTO YUMI, MATSUMOTO KAZUYA, AMANO TOMOKO, KURIHARA TAKASHI, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, 日本分子生物学会年会プログラム・講演要旨集, 27th, 714,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902279039552902
  • マウス体細胞核移植はいにおける微小管の変化, KAWASUMI MIYURI, ANZAI MASAYUKI, KUNIEDA TAKANORI, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 27th, 537,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902292351200998
  • マウス初期はいにおける時計遺伝子群の発現解析, MATSUSHITA SATONORI, AMANO TOMOKO, MATSUMOTO KAZUYA, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, J Reprod Dev, 50, Supplement, J57,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902214844920620
  • マウス初期はいのはい性遺伝子発現機構におけるDNAメチル化及びヒストンアセチル化の関与, YAMAMOTO YUMI, MATSUMOTO KAZUYA, AMANO TOMOKO, KURIHARA TAKASHI, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, J Reprod Dev, 50, Supplement, J59,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902283953789853
  • 初期G1期のウシ初代繊維芽細胞における遺伝子発現および核の活性状態と核移植後の発生の検討, KASAMATSU REI, SAEKI KAZUHIRO, TAMASATO TOMOHIRO, MITANI MASASHI, HOSOI YOSHIHIKO, TANIGUCHI TOSHIHITO, IDE ATSUSHI, URAKAWA MAMI, AOYAGI NORITO, J Reprod Dev, 50, Supplement, J63, j63,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902246203300175
  • マウス初期胚における時計遺伝子群の発現解析, 松下 聡紀, 天野 朋子, 松本 和也, 安齋 政幸, 三谷 匡, 加藤 博己, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 50, Suppl., j57, j57,   2004 08
  • マウス初期胚で発現するrhophilin-2遺伝子と相互作用する因子の探索, 松岡 俊樹, 松本 和也, 天野 朋子, 安齋 政幸, 三谷 匡, 加藤 博己, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 50, Suppl., j58, j58,   2004 08
  • マウス初期胚の胚性遺伝子発現機構におけるDNAメチル化及びヒストンアセチル化の関与, 山本 由美, 松本 和也, 天野 朋子, 栗原 隆, 安齋 政幸, 三谷 匡, 加藤 博己, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 50, Suppl., j59, j59,   2004 08
  • 植物由来脂肪酸不飽和化酵素を発現させたトランスジェニックマウスにおける遺伝子挿入部位の解析, 新海 雄介, 湯浅 一之, 桐本 真治, 松本 和也, 天野 朋子, 安斎 政幸, 三谷 匡, 加藤 博己, 田口 善智, 細井 美彦, 佐伯 和弘, 入谷 明, The Journal of Reproduction and Development, 50, Suppl., j94, j94,   2004 08
  • ルシフェラーゼ遺伝子を導入したウシ線維芽細胞を用いたクローンはいにおける遺伝子発現がその後の初期発生に及ぼす影響, TAMASATO TOMOHIRO, KASAMATSU REI, SHIROMIZU KOICHIRO, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, TANIGUCHI SHUNJI, IRIYA AKIRA, 日本畜産学会大会講演要旨, 103rd, 113,   2004 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902247553299362
  • ウシ体細胞クローン胚における機能的遺伝子の転写調節領域のCpGメチル化, 栗原 隆, 松本 和也, 富永 敬一郎, 安齋 政幸, 三谷 匡, 加藤 博己, 佐伯 和弘, 細井 美彦, 入谷 明, 日本畜産学会大会講演要旨集, 103回, 112, 112,   2004 03
  • DD2-2, a Gonad-Specific Gene, Is Involved in the Formation of 20S Proteasome in Early Pre-Implantation Embryo, Seung-Wook Shin, Mikiko Tokoro, Satoshi Nishikawa, Yuki Hatanaka, Tomoko Amano, Satoshi Kishigami, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Intam, Kazuya Matsumoto, BIOLOGY OF REPRODUCTION, 125, 125,   2009
  • Oocyte Activation in Mice Using Strontium with Calcium-Selective Chelators, Yoshiko Tsujimoto, Satoshi Kishigami, Toshiyuki Takehara, Masayuki Anzai, Tasuku Mitani, Hiromi Kato, Tomoko Amano, Kazuya Matsumoto, Kazuhiro Saeki, Akira Iritani, Yoshihiko Hosoi, BIOLOGY OF REPRODUCTION, 191, 191,   2009
  • Viability and DNA fragmentation of mouse frozen bone marrow cells without cryoprotectant and its availability for nuclear donor in somatic cell nuclear transfer, H. Kato, A. Nakao, M. Nishiwaki, M. Anzai, T. Mitani, A. Iritani, REPRODUCTION IN DOMESTIC ANIMALS, 43, 191, 192,   2008 07
  • Expression of the genes implicated in the development of the trophoblast lineage in mouse somatic cell nuclear transfer embryos, T. Mitani, M. Nishiwaki, M. Morita, K. Moriki, Y. Nishiyama, H. Kato, M. Anzai, A. Iritani, REPRODUCTION IN DOMESTIC ANIMALS, 43, 194, 194,   2008 07
  • Transgenic SiRNA halts ALS in a mouse model, Taknori Yokota, Hiroki Sasaguri, Hidehiro Mizusawa, Tasuku Mitani, NEUROLOGY, 68, 12, A403, A403,   2007 03
  • Increase of disease duration of amyotrophic lateral sclerosis in a mouse model by transgenic small interfering RNA, Takanori Yokota, Hiroki Sasaguri, Yuki Saito, Hiromi Yamada, Toshinori Unno, Yuki Yamamoto, Takayuki Kubodera, Masayuki Anzai, Tasuku Mitani, Hidehiro Mizusawa, ARCHIVES OF NEUROLOGY, 64, 1, 145, 146,   2007 01
  • Bovine somatic cell nuclear transfer embryos: Effects of the gene expression ability and DNA methylation on their development to the blastocyst stage., Aya Kasamatsu, Kazuhiro Saeki, Daisaku Iwamoto, Shunji Taniguchi, Tasuku Mitani, Hiromi Kato, Yoshito Aoyagi, Manami Urakawa, Atsushi Ideta, Kazuya Matsumoto, Yoshihiko Hosoi, Akira Iritani, BIOLOGY OF REPRODUCTION, 111, 112,   2006

Awards & Honors

  • The Society for Reproduction and Development, JRD Outstanding Paper Award 2017, Visualization of the spatial arrangement of nuclear organization using three-dimensional in situ hybridization in early mouse embryos: A new "EASI-FISH chamber glass" for mammalian embryos.

Research Grants & Projects

  • SEI Group CSR Foundation, Support for academic and research activities, Dynamics of chromosome territories and gene loci in the nucleus of nuclear transfer eggs in mice.
  • JST, JST Comprehensive Support Programs for Creation of Regional Innovation, Isolation and Culture of Embryonic Pluripotential Stem Cells and Production of Their Cloned Animals
  • Grant-in-Aid for Scientific Research, Isolation of pluripotential somatic stem cells for production of somatic clone mice.
  • Isolation and Culture of Pluripotential Stem Cells

Educational Activities

Teaching Experience

  • Basic Life and Science of Animals and Human Beings, Graduate School of Biology-oriented Science and Technology, Kindai University
  • Advanced Stem Cell Engineering, Graduate School of Biology-oriented Science and Technology, Kindai University
  • Introduction of Life Science, Department of Biology-oriented Science and Technology, Kindai University
  • Animal Physiology, Department of Biology-oriented Science and Technology, Kindai University
  • Stem Cell Engineering for Regenerative Medicine, Department of Biology-oriented Science and Technology, Kindai University
  • Outline of Immunology, Department of Biology-oriented Science and Technology, Kindai University
  • Animal Reproduction, Department of Biology-oriented Science and Technology, Kindai University