KINDAI UNIVERSITY


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TAKEBE So

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FacultyDepartment of Science and Technology on Food Safety / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/329-takebe-sou.html
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Last Updated :2020/09/14

Education and Career

Education

  •  - 1989 , Kyoto University, Graduate School, Division of Agriculture
  •  - 1984 , Kyoto University, Faculty of Agriculture
  •   1980 04  - 1984 03 , Kyoto University, Faculty of Agriculture

Academic & Professional Experience

  •   1997 ,  - 2005 , Faculty of Biology-Oriented Science and Technology, Kindai University
  •   1989 ,  - 1990 , University of Tsukuba
  • Kyoto Women's University
  •   1997 ,  - 2005 , Associate Professor,
  •   2005 , - Professor,
  •   1990 ,  - 1997 , Lecturer, Dept. of Food Science,
  •   1989 ,  - 1990 , Technical Officer, University of Tsukuba
  • Kinki University
  • Dept. of Genetic Engineering,
  • Kinki University
  • Dept. of Genetic Engineering,

Research Activities

Research Areas

  • Environmental science/Agricultural science, Conservation science (plants)
  • Environmental science/Agricultural science, Conservation science (plants)
  • Life sciences, Applied biochemistry
  • Life sciences, Applied microbiology

Research Interests

  • insecticidal protein, pore-forming toxin, Protein Engineering, Regulation of Gene Expression

Published Papers

  • Characterization of the channel‑pores formed by Bacillus thuringiensis Cry46Ab toxin in planar lipid bilayers, Akira Sakakibara, So Takebe, Toru Ide, Tohru Hayakawa, Applied Entomology and Zoology, Applied Entomology and Zoology, 54, 389 - 398, Aug. 2019 , Refereed
  • Potency of the mosquitocidal Cry46Ab toxin produced using a 4AaCter-tag, which facilitates formation of protein inclusion bodies in Escherichia coli, Tomoaki Okazaki, Junya Ichinose, So Takebe, Toru Ide, Tohru Hayakawa, Applied Entomology and Zoology, Applied Entomology and Zoology, 53(1), 67 - 73, Feb. 01 2018 , Refereed
    Summary:A Cry46Ab toxin derived from Bacillus thuringiensis strain TK-E6 shows mosquitocidal activity against Culex pipiens pallens Coquillett (Diptera: Culicidae) larvae as well as preferential cytotoxicity against human cancer cells. In B. thuringiensis cells, Cry46Ab is produced and accumulates as a protein crystal that is processed into the active 29-kDa toxin upon solubilization in the alkaline environment of the insect midgut. The Cry46Ab protoxin is 30 kDa, and is therefore thought to require an accessory protein such as P20 and/or ORF2 for efficient crystal formation. In the present study, the potency of the 4AaCter-tag was investigated for the production of alkali-soluble inclusion bodies of recombinant Cry46Ab in Escherichia coli. The 4AaCter-tag is a polypeptide derived from the C-terminal region of the B. thuringiensis Cry4Aa toxin and facilitates the formation of alkali-soluble protein inclusion bodies in E. coli. Fusion with the 4AaCter-tag enhanced both Cry46Ab production and the formation of Cry46Ab inclusion bodies. In addition, upon optimization of protein expression procedures, the Cry46Ab–4AaCter inclusion bodies showed mosquitocidal activity and stability in aqueous environments comparable to Cry46Ab without the 4AaCter-tag. Our study suggests that use of the 4AaCter-tag is a straightforward approach for preparing formulations of smaller-sized Cry toxins such as Cry46Ab in E. coli.
  • A search for novel cry genes of Bacillus thuringiensis by degenerate PCR, Karatani, Y, Takebe, S, Mem. Faculty BOST Kinki Univ., Mem. Faculty BOST Kinki Univ., (24), 27 - 32, Sep. 2009 , Refereed
  • Improved technique for refining the crystal of Bacillus thuringiensis by NaBr gradient centrifugation, Takebe, S, Morinaga, S, Mizuhashi, A, Komano, T, Proceedings of 6th Pacific Rim Conference on BT, Proceedings of 6th Pacific Rim Conference on BT, 111 - 112, Mar. 2008
  • Characterization of novel cry genes isolated from a mosquitocidal Bacillus thuringiensis strain, Takebe, S, Matsumura, T, Mizuhashi, A, Yokoo, N, Komano, T, Yamagiwa, M, Abdullah, M.A.F, Dean, D.H, Biotechnology of Bacillus thuringiensis, Biotechnology of Bacillus thuringiensis, 5, 125 - 127, Aug. 2005
  • Construction of new Bt expression vector, pPcyt1A, Takebe, S, Yokoo, N, Mizuhashi, A, Matsumura, T, Komano, T, Mem. Inst. Ad. Tech., Kinki Univ., Mem. Inst. Ad. Tech., Kinki Univ., (10), 29 - 36, Mar. 2005
  • Degenerate PCR based approach for detection of novel cry genes, Takebe, S, Yamane, Y, Isumoto, Y, Komano, T, Mem. Inst. Ad. Tech., Kinki Univ., Mem. Inst. Ad. Tech., Kinki Univ., (7), 34 - 39, Jan. 2002
  • Degenerate PCR-based detection of novel cry genes in soil bacteria, Takebe, S, Isumoto, Y, Matsumura, T, Sugiura, K, Yamane, Y, Sen, K, Komano, T, Proceedings of 4th Pacific Rim Conference on BT, Proceedings of 4th Pacific Rim Conference on BT, 138 - 139, 2002
  • Cloning and sequencing of cDNA that encodes ostrich insulin-like growth factor-I, Komano, T, Takebe, S, Sakai, H, Mem. School BOST Kinki Univ., Mem. School BOST Kinki Univ., (6), 38 - 46, Feb. 2000 , Refereed
  • Cloning and Sequencing of cDNA that encodes Ostrich Growth Hormone, Komano, T, Takebe, S, Sakai, H, Mem. School BOST Kinki Univ., Mem. School BOST Kinki Univ., (5), 74 - 81, Oct. 1999 , Refereed
  • Oxidative stress response of acrAB promoter, Takebe, So, Mizuno, Tamaki, Yuki, Hitomi, Mem. School BOST Kinki Univ., Mem. School BOST Kinki Univ., (4), 73 - 79, Dec. 1998 , Refereed
  • Isolation of DNA fragments containing promoter function inducible by hydrogen peroxide, Tkebe, S, Aratake, M, Kuroda, N, Yamamoto, A, Yoshida, A, J. Food & Nutr. Inst. Kyoto Women's Univ., J. Food & Nutr. Inst. Kyoto Women's Univ., (51), 33 - 39, Dec. 1996 , Refereed
  • Effect of cAMP-CRP regulation on sodA promoter activity, Takebe, S, Azuma, M, Yoshida, M, Yoshida, A, J. Food & Nutr. Inst. Kyoto Women's Univ., J. Food & Nutr. Inst. Kyoto Women's Univ., (50), 37 - 42, Dec. 1995 , Refereed
  • Divergent promoter, existed in oxyR promoter region, is hydrogen peroxide-inducible, Yoshida, A, Takebe, S, J. Food & Nutr. Inst. Kyoto Women's Univ., J. Food & Nutr. Inst. Kyoto Women's Univ., (48), 22 - 28, Dec. 1993 , Refereed
  • DNA damage induced by active oxygens, Takebe, So, J. Food & Nutr. Inst. Kyoto Women's Univ., J. Food & Nutr. Inst. Kyoto Women's Univ., (45), 10 - 13, Dec. 1990 , Refereed
  • Induction of DNA damage and mutation by active oxygens, TAKEBE, So, Doctoral Thesis, Doctoral Thesis, 1 - 58, Jan. 1990
  • DNA damage caused by ascorbate in the presence of Cu2+ induces mutations, Kobayashi, Takebe), S, Yoshida, K, Ueda, K, Sakai, H, Komano, T, Nucleic Acids Symp. Ser. Articles, Nucleic Acids Symp. Ser. Articles, (19), 29 - 32, 1988
  • Cleavage of Stem-and- loop structure DNA by bleomycin : Reaction on the bacteriophage G4 Origin of complementary strand synthesis, Ueda, K, Kobayashi, Takebe), S, Sakai, H, Komano, T, The Journal of Biological Chemistry, The Journal of Biological Chemistry, 260(9), 5804 - 580, 1985 , Refereed
  • Site-specific DNA damage caused by lipid peroxidation products, Ueda, K, Kobayashi, Takebe) S, Morita, J, Komano, T, Biochimica et Biophysica Acta, Biochimica et Biophysica Acta, 824, 341 - 346, 1985 , Refereed
  • Bleomycin cleaves DNA depending on DNA primary, secondary, and tertiary structures, Ueda, K, Kobayashi, Takebe), S, Komano, T, Nucleic Acids Symp. Ser. Articles, Nucleic Acids Symp. Ser. Articles, (16), 197 - 200, 1985
  • Cry46Ab from Bacillus thuringiensis TK-E6 is a new mosquitocidal toxin with aerolysin-type architecture, Tohru Hayakawa, Akira Sakakibara, Sho Ueda, Yoshinao Azuma, Toru Ide, So Takebe, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 87, 100 - 106, Aug. 2017 , Refereed
    Summary:Cry46Ab is a Cry toxin derived from Bacillus thuringiensis TK-E6. Cry46Ab is not significantly homologous to other mosquitocidal Cry or Cyt toxins and is classified as an aerolysin-type pore-forming toxin based on structural similarity. In this study, the potency of Cry46Ab was assessed for its potential application to mosquito control. A synthetic Cry46Ab gene, ciy46Ab-S1, was designed to produce recombinant Cry46Ab as a glutathione-S-transferase fusion in Escherichia coli. Recombinant Cry46Ab showed apparent toxicity to Culex pipiens larvae, with a 50% lethal dose of 1.02 mu g/ml. In an artificial lipid bilayer, Cry46Ab activated by trypsin caused typical current transitions between open and closed states, suggesting it functions as a pore-forming toxin similar to other Cry and Cyt toxins. The single-channel conductance was 103.3 +/- 4.1 pS in 150 mM KCl. Co-administration of recombinant Cry46Ab with other mosquitocidal Cry toxins, especially the combination of Cry4Aa and Cry46Ab, resulted in significant synergistic toxicity against C. pipiens larvae. Co-administration of multiple toxins exhibiting different modes of action is believed to prevent the onset of resistance in insects. Our data, taken in consideration with the differences in its structure, suggest that Cry46Ab could be useful in not only reducing resistance levels but also improving the insecticidal activity of Bt-based bio-insecticides. (C) 2017 Elsevier Ltd. All rights reserved.
  • Draft genomic DNA sequence of the facultatively methylotrophic bacterium Acidomonas methanolica type strain MB58, Norie Higashiura, Hiromi Hadano, Hideki Hirakawa, Minenosuke Matsutani, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, FEMS MICROBIOLOGY LETTERS, FEMS MICROBIOLOGY LETTERS, 351(1), 9 - 13, Feb. 2014 , Refereed
    Summary:Acidomonas methanolica (former name: Acetobacter methanolicus) is a unique acetic acid bacterium capable of growing on methanol as a sole carbon source. We reported the draft genome sequencing of A.methanolica type strain MB58, showing that it contains 3270 protein-coding genes, including the genes involved in oxidation of methanol, such as mxaFJGIRSACKL and hxlAB, and oxidation of ethanol, such as adhAB and adhS.
  • Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis, Tohru Hayakawa, Rie Kanagawa, Yosuke Kotani, Mayumi Kimura, Masashi Yamagiwa, Yoshiharu Yamane, So Takebe, Hiroshi Sakai, CURRENT MICROBIOLOGY, CURRENT MICROBIOLOGY, 55(4), 278 - 283, Oct. 2007 , Refereed
    Summary:A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC50 values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.
  • Transcription of the insecticidal crystal protein genes of Bacillus thuringiensis, Tohru Komano, So Takabe, Hiroshi Sakai, Biotechnology Annual Review, Biotechnology Annual Review, 5, 131 - 154, 2000 , Refereed
    Summary:Production of a large amount of insecticidal crystal proteins encoded on large plasmids is largely dependent upon the mother cell, Bacillus thuringiensis (B. thuringiensis, also Bt), specific transcription systems attributable to sporulation. In the middle stages of sporulation, cry4A is most actively transcribed from the promoter cry4A-P1. The proximal transcriptional start point of cry4A, which is under the control of the promoter P1, is used in Bacillus subtilis (B. subtilis) in the middle stage of sporulation. The nucleotide sequence that determines the cry4A-P1 promoter is homologous to the consensus sequence for the promoter of σE-specific genes in B. subtilis, and to those promoters of the insecticidal protein genes that are efficiently transcribed in vitro with the RNA polymerase Eσ35 isolated from B. thuringiensis. The σ factor σ35 of B. thuringiensis is highly homologous and functionally equivalent to σE of B. subtilis. These results suggest that the cry4A transcription from P1 is under the control of σE in B. subtilis, and under the control of σ35 in B. thuringiensis. ©2000 Elsevier Science B.V. All rights reserved.
  • FUNCTIONAL EXPRESSION OF HUMAN P-GLYCOPROTEIN IN SCHIZOSACCHAROMYCES-POMBE, K UEDA, AM SHIMABUKU, H KONISHI, Y FUJII, S TAKEBE, K NISHI, M YOSHIDA, T BEPPU, T KOMANO, FEBS LETTERS, FEBS LETTERS, 330(3), 279 - 282, Sep. 1993 , Refereed
    Summary:Human MDR1 cDNA was introduced into the human cultured cells KB-3-1 and Schizosaccharomyces pombe pmd1 null mutant KN3. The drug sensitivity of KB-G2 and KN3/pgp, expressing human P-glycoprotein, was examined. KB-G2 was resistant to the peptide antibiotics valinomycin and gramicidin D as well as having a typical multidrug resistance (MDR) phenotype. KN3/pgp was resistant to valinomycin and actinomycin D, but not to adriamycin. The ATP-hydrolysis-deficient mutant did not confer KN3 resistance to these antibiotics. Human P-glycoprotein expressed in S. pombe seemed to lack N-glycosylation. The N-glycosylation-deficient mutant, however, conferred a typical MDR phenotype on KB-3-1. These results suggest that human P-glycoprotein functions as an efflux pump of valinomycin and actinomycin D in the membrane of S. pombe.
  • MUTATIONAL ANALYSIS OF THE SPECIFIC PRIMING SIGNAL ESSENTIAL FOR DNA-REPLICATION OF THE BROAD-HOST-RANGE PLASMID RSF1010, Y HONDA, T AKIOKA, S TAKEBE, K TANAKA, D MIAO, A HIGASHI, T NAKAMURA, Y TAGUCHI, H SAKAI, T KOMANO, M BAGDASARIAN, FEBS LETTERS, FEBS LETTERS, 324(1), 67 - 70, Jun. 1993 , Refereed
    Summary:To analyze the RSF1010-specific priming mechanism, a library of randomly mutagenized ssiA sequences was constructed by chemical synthesis using mixed nucleotide phosphoramidites. Synthetic ssiA sequences with the single base-substitutions were assayed for the SSI activity in E. coli JM109 expressing RepB' primase. It was demonstrated that the activity of ssiA was damaged markedly by single base-substitutions within the possible stem-loop structure and its 3'-flanking region. It is conceivable that these domains are critical in recognition and primer synthesis by RepB' primase.
  • ALTERNATIVE SECONDARY STRUCTURES IN THE PHAGE-G4 ORIGIN OF THE COMPLEMENTARY-DNA STRAND SYNTHESIS - EFFECTS OF NACL CONCENTRATION ON THE BLEOMYCIN DNA INTERACTION, K UEDA, H HIASA, S TAKEBE, H SAKAI, T KOMANO, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 56(3), 394 - 398, Mar. 1992 , Refereed
    Summary:The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA strand synthesis.
  • THE EFFECTS OF METAL-IONS ON THE DNA DAMAGE INDUCED BY HYDROGEN-PEROXIDE, S KOBAYASHI, K UEDA, T KOMANO, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 54(1), 69 - 76, Jan. 1990 , Refereed
  • DNA Damage induced by free radicals generated from hydroperoxides, Medical, Biochemical and Chemical Aspects of Free Radicals, Medical, Biochemical and Chemical Aspects of Free Radicals, 2, 1989
  • DNA DAMAGE INDUCED BY ASCORBATE IN THE PRESENCE OF CU-2+, S KOBAYASHI, K UEDA, J MORITA, H SAKAI, T KOMANO, BIOCHIMICA ET BIOPHYSICA ACTA, BIOCHIMICA ET BIOPHYSICA ACTA, 949(1), 143 - 147, Jan. 1988 , Refereed
  • SEQUENCE-SPECIFIC MODIFICATION OF DNA BY 6-HYDROXYBENZO[A]PYRENE, J MORITA, K UEDA, S KOBAYASHI, T KOMANO, BIOCHIMICA ET BIOPHYSICA ACTA, BIOCHIMICA ET BIOPHYSICA ACTA, 868(1), 87 - 90, Oct. 1986 , Refereed

Books etc

  • Frontiers of Reactive Oxygen Species in Biology and Medicine, Takebe, S, Yoshida, A, Saeki, T, Sakai, H, Komano, T, Contributor, Effects of metal ions on the phage inactivation reaction caused by active oxygens, Elsevier Science Publishers B.V., Amsterdam,   1994
  • Medical, Biological and Chemical Aspects of Free Radicals, vol 2, Kobayashi, Takebe), S, Ueda, K, Morita, J, Komano, T, Contributor, DNA damage induced by free radicals generated from hydroperoxide, Elsevier Scince Publishers B.V., Amsterdam,   1989

Conference Activities & Talks

  • Microbial protein toxins that are toxic to apple snails and mosquito larvae, Nakagawa, Nao, Nishikaku, Saki, Azuma, Yoshinao, Hayakawa, Tohru, TAKEBE, So, 52nd Annual Meeting of the Society for Invertebrate Pathology,   2019 07 31
  • Mosquitocidal activity of parasporin type 33-kDa Cry protein from Bacillus thuringiensis isolated in Japan, Sho Ueda, Ayako Negoro, Masatoshi Ota, Ami Kurokawa, Jun Makimoto, Yoshinao Azuma, Tohru Hayakawa, So TAKEBE, 15th International Congress of Bacteriology and Applied Microbiology,   2017 07 17
  • IDENTIFICATION OF A NEW HALOMONAS GROUP FROM WASTE LIQUIDS OF UMEBOSHI (JAPANESE PICKLED PLUM) FACTORY, Ayaka Tsuji, Tsubasa Kiji, Hitomi Handa, Kaho Okada, So Takebe, Yoshinao Azuma, IUMS2017,   2017 07
  • ESTABLISHMENT OF IN VITRO INFECTION BY PLASMODIOPHORA BRASSICAE, CAUSING CLUBROOT OF BRASSICA RAPA, Kaho Okada, Kimie Hayasaki, Ayaka Kushitani, Ayaka Tsuji, So Takebe, Yoshinao Azuma, IUMS2017,   2017 07
  • Mosquitocidal activity of non-3-domain Cry type 33-kDa protein from Bacillus thuringiensis isolated in Japan, Sho Ueda, Ayako Negoro, Masatoshi Ota, Ami Kurokawa, Jun Makimoto, Yoshinao Azuma, Tohru Hayakawa, So Takebe, 49th Annual Meeting of the Society for Invertebrate Pathology,   2016 07
  • Omics analyses for Hyper Glucose-Tolerance using Acetic Acid Bacteria, TAKEBE So, American Institute of Chemical Engineers (AIChE),   2016 06
  • Molecular Engagements for Hyper Glucose-Tolerance of Acetic Acid Bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis, TAKEBE So, The American Society for Microbiology (ASM),   2016 06
  • Gene organization of cry genes on large plasmids of novel mosquitocidal Bacillus thuringensis TK-E6, Sho UEDA, Kanae TOMITA, Kaori INAMORI, Mayu NODA, Yoshinao AZUMA, So TAKEBE,   2016 03 30
  • Complete genome and RNA-seq analyses of Asaia bogorensis reveal unique response to culture with mammalian cells, 2nd International Symposium Matryoshka-type Evolution of Eukaryotic Cells,   2015 09
  • Genome and RNA-seq analyses of Asaia bogorensis reveal adaptive responses as an opportunistic pathogen,   2014 12 05
  • Unique stress-responsible genes of alphaproteobacterial Asaia bogorensis revealed by whole genome sequencing and RNA-seq, Mikihiko Kawai, Norie Higashiura, Kimie Hayasaki, Naruhei Okamoto, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma,   2014 11 25
  • Gene organization of large plasmids of novel mosquitocidal Bacillus thuringiensis TK-E6, Mayu Noda, Naruhei Okamoto, Kimie Hayasaki, Yoshinao Azuma, So Takebe, 2014 International Congress on Invertebrate Pathology and Microbial Control & 47th Annual Meeting of the Society for Invertebrate Pathology,   2014 08 , SIP
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis,   2013 12 05
  • Draft Genomic DNA Sequence of the Facultatively Methylotrophic Bacterium Acidomonas methanolica type strain MB58, Norie Higashiura, Hiromi Hadano, Hideki Hirakawa, Minenosuke Matsutani, Ryoji Mitsui, Sou Takebe, Kazunobu Matsushita, Yoshinao Azuma,   2013 10
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Norie Higashiura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma,   2013 03 27
  • Genome analyses for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma,   2012 12 14
  • Genome analyses for hyper glucose tolerance of an acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis, Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma,   2012 11 10
  • Genome analysis for hyper glucose tolerance of acetic acid bacteria, Tanticharoenia sakaeratensis and Asaia bogorensis., Hiromi Hadano, Kasumi Omura, Hideki Hirakawa, So Takebe, Kazunobu Matsushita, Yoshinao Azuma, 3rd International Meeting of Acetic Acid Bacteria,   2012 04 12
  • Genome and Proteome analyses for hyper glucose tolerance of an acetic acid bacterium, Tanticharoenia sakaeratensis, Hiromi Hadano, So Takebe, Yoshinao Azuma, MBSJ 2011,   2011 12 14
  • Genome and proteome analyses for hyper glucose tolerance of an acetic acid bacterium, Tanticharoenia sakaeratensis, Hiromi Hadano, Yuta Sugimori, So Takebe, Yoshinao Azuma, The IUMS 2011 Sapporo Congress,   2011 09 07
  • Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis, Yuta Sugimori, Yu Karatani, Hiromi Hadano, Yoshinao Azuma, So Takebe, The IUMS 2011 Sapporo Congress,   2011 09 07
  • Degenerate PCR based search for genes encoding insecticidal proteins of Bacillus thuringiensis, Yu Karatani, Yoshinao Azuma, So Takebe, 43rd Annual meeting of the society for invertebrate pathology,   2010 07 , 招待有り
  • A novel cytotoxic protein MM29kD from Bacillus thuringiensis, 20th IUBMB International Congress of Biochemistry and Molecular Biology,   2006 06 , 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Characterization of novel cry genes from mosquitocidal Bacillus thuringiensis strain TK-E6, 20th IUBMB International Congress of Biochemistry and Molecular Biology,   2006 06 , 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Improved technique for refining the crystal of Bacillus thuringiensis by NaBr gradient centrifugation, 6th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis,   2005 11 , 6th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis
  • Mutational analysis of the C-terminal half of a novel cytotoxic protein MM29kD,   2004 12
  • Cytotoxicity against mammalian cells of a novel crystal protein from Bacillus thuringiensis,   2004 12
  • Characterization of Novel cry Genes Isolated from Mosquitocidal Bacillus thuringiensis Strain,   2003 11
  • Research on Mosquitocidal Toxins from Bacillus thuringiensis,   2003 09
  • Degenerate PCR based detection of novel cry genes in the soil bacteria,   2001 11

Works

  • Isolation and characterization of novel Ñ∞DBBacillus thuringiensis(/)-Ñ≤ DB strains carring insecticidal genesˇ

Research Grants & Projects

  • Analysis of Bacillus thuringiensis insecticidal protein genes‹
  • Isolation and characterization of genes inducible by active oxygens