 | 松井 一彰 （マツイ カズアキ）
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水銀耐性細菌を指標に、細菌の分散・分布や遺伝子水平伝播現象を研究しています。実験生態系を用いた生物多様性と生態系多機能性の解明を試みています。
研究者情報
学位
ホームページURL
J-Global ID
研究キーワード
- 下水の微生物 遺伝子水平伝播 薬剤耐性細菌 水銀耐性細菌 都市河川 マイクロコズム 重金属耐性 生物多様性
現在の研究分野（キーワード）
- 水銀耐性細菌を指標に、細菌の分散・分布や遺伝子水平伝播現象を研究しています。実験生態系を用いた生物多様性と生態系多機能性の解明を試みています。
研究分野
経歴
- 2019年 - 2020年 ウィスコンシン大学ミルウォーキー校School of Freshwater Science客員教授
- 2006年 - 2007年 総合地球環境学研究所・研究部・上級研究員
- 2003年 - 2006年 日本学術振興会特別研究員 (PD)(東北学院大学・工)
- 2002年 - 2003年 京都大学生態学研究センター 教務補佐員Center for Ecological Research
- 2000年 - 2002年 日本学術振興会特別研究員 (DC2)(京都大学)
- 1995年 - 1996年 イリノイ大学シカゴ校 微生物・免疫学科 研究員
- 1994年 - 1995年 オークランド大学 生物科学部 研究員
学歴
- - 2002年 京都大学 理学研究科 生物科学
- - 2001年 Department of Microbiology and Immunology, University of Illinois at Chicago
- - 1994年 愛媛大学 農学研究科
- - 1992年 愛媛大学 農学部 生物資源学科
所属学協会
研究活動情報
論文
- Kazuaki Matsui; Takeshi MikiPeerJ 11 e14684 - e14684 2023年01月 [査読有り]
When the wastewater volume exceeds the sewer pipe capacity during extreme rainfall events, untreated sewage discharges directly into rivers as combined sewer overflow (CSO). To compare the impacts of CSOs and stormwater on urban waterways, we assessed physicochemical water quality, the 16S rRNA gene-based bacterial community structure, and EcoPlate-based microbial functions during rainfall periods in an urban waterway before and after a stormwater storage pipe was commissioned. A temporal variation analysis showed that CSOs have significant impacts on microbial function and bacterial community structure, while their contributions to physicochemical parameters, bacterial abundance, and chlorophyll a were not confirmed. Heat map analysis showed that the impact of CSO on the waterway bacterial community structure was temporal and the bacterial community composition in CSO is distinct from that in sewers. Hierarchical clustering analysis revealed that the waterway physicochemical water qualities, bacterial community composition, and microbial community function were distinguishable from the upper reach of the river, rather than between CSO and stormwater. Changes in the relative abundance of tetracycline resistance (tet) genes—especially tet(M)—were observed after CSOs but did not coincide with changes in the microbial community composition, suggesting that the parameters affecting the microbial community composition and relative abundance of tet genes differ. After pipe implementation, however, stormwater did not contribute to the abundance of tet genes in the waterway. These results indicate that CSO-induced acute microbial disturbances in the urban waterway were alleviated by the implementation of a stormwater storage pipe and will support the efficiency of storage pipe operation for waterway management in urban areas. - 寺田 千里; 中村 誠宏; 朱 詩瑶; 井口 光; 高木 惇司; 松井 一彰; 柴田 英昭森林立地 64 2 83 - 90 森林立地学会 2022年12月 [査読有り]
炭素は微生物の増殖に関わる重要な元素であるため，炭素基質の利用に関わる機能（機能の多様性と多機能性）の違いは土壌微生物群集を特徴づける指標となる。土壌微生物群集の機能の多様性と多機能性を評価するために，群集レベルでの炭素基質利用機能の生理的プロファイリング法（CLPP：Community level physiological profiles）が用いられている。森林の土壌微生物群集の機能を測定するCLPP手法の一つとして，BIOLOGTM社が開発したエコプレートがある。本研究では，森林土壌を扱ったエコプレート実験を始めようと考えている様々な立場の方々（高校教員，大学生，一般市民，研究者）に，参考となる測定条件の情報を提供することを目的とした。参考となる土壌深度，希釈倍率，風乾期間，吸光度の測定期間を解明するために，和歌山県の典型的な照葉樹二次林の褐色森林土を使って異なる測定条件でエコプレート実験を行った。その結果，本調査地においては表層から10 cm程度の土壌層から土壌を採集して，風乾はせずに（やむを得ない場合，測定まで4 ℃程度の冷蔵庫で保管），抽出液は50倍，100倍，200倍の希釈倍率の間で，吸光度の測定は10～14日間かけて実施することが，土壌微生物群集の機能の多様性と多機能性を正確に評価できる妥当な条件であることを示した。 - M. Nakamura; C. Terada; K. Ito; K. Matsui; S. Niwa; M. Ishihara; T. Kenta; T. Yoshikawa; T. Kadoya; T. Hiura; H. Muraoka; K. Ishida; N. Agetsuma; R. Nakamura; H. Sakio; M. Takagi; A. S. Mori; M. K. Kimura; H. Kurokawa; T. Enoki; T. Seino; A. Takashima; H. Kobayashi; K. Matsumoto; K. Takahashi; R. Tateno; T. Yoshida; T. Nakaji; M. Maki; K. Kobayashi; K. Fukuzawa; K. Hoshizaki; K. Ohta; K. Kobayashi; M. Hasegawa; S. N. Suzuki; M. Sakimoto; Y. Kitagawa; A. Sakai; H. Kondo; T. Ichie; K. Kageyama; A. Hieno; S. Kato; T. Otani; Y. Utsumi; T. Kume; K. Homma; K. Kishimoto; K. Masaka; K. Watanabe; M. Toda; D. Nagamatsu; Y. Miyazaki; T. Yamashita; N. TokuchiEcological Research 37 3 432 - 445 2022年 [査読有り]
We present the largest freely available EcoPlate dataset for Japan, comprising data collected from a network of 33 natural forest sites (77 plots) in regions of East Asia ranging from cool temperate to subtropical. EcoPlate is a 96-well microplate that contains three repeated sets of 31 response wells with different sole carbon substrates. The utilization of each carbon substrate by the microbial community is quantified by the color density of the well during incubation. EcoPlate can provide a multifunctional index of a soil microbial community. Soil properties (water content, carbon [C] and nitrogen [N] contents, the C/N ratio, and pH) that are essential for interpreting the EcoPlate results were also measured. The network is part of the Forest and Grassland Survey of the Monitoring Sites 1000 Project established by the Ministry of the Environment. Using a standardized protocol, soil was sampled between September and December 2020. A preliminary principal component analysis was performed on the temporal integration of color density using 31 substrates. For the temporal integration, we calculated the cumulative amount of color development by integrating the color density development curve. PC1 contributed 55.6% and is thought to represent the magnitude of the overall absorbance of all substrates. The model of environmental factors including elevation and the model of soil properties, including water content and pH, were selected as the best-fit models for variation in PC1. EcoPlate data enable the meta-analysis of comparative studies among forest types and testing community ecology and ecosystem function hypotheses at broad spatial scales. The complete data set for this abstract published in the Data Paper section of the journal is available in electronic format in MetaCat in JaLTER at http://db.cger.nies.go.jp/JaLTER/metacat/metacat/ERDP-2022-01.1/jalter-en. - Disproportionate Changes in Composition and Molecular Size Spectra of Dissolved Organic Matter between Influent and Effluent from a Major Metropolitan Wastewater Treatment PlantHui Lin; Kazuaki Matsui; Ryan J Newton; Laodong GuoACS ES&T Water 2 216 - 225 2022年 [査読有り]
- Kazuaki Matsui; Takayuki Fumoto; Hiroshi KawakamiLimnology 20 131 - 136 2019年 [査読有り]
- Kazuaki Matsui; Ginro EndoApplied Microbiology and Biotechnology 102 7 3037 - 3048 2018年04月 [査読有り]
Mercury-resistant (HgR) bacteria occur in various bacterial species from a wide variety of environmental sources. Resistance is conferred by a set of operon genes termed the mer operon. Many HgR bacteria have been isolated from diverse environments and clinical samples, and it is recognized that mer operons are often localized on transposons. Previous research reports have suggested that HgR transposons participate in the horizontal gene transfer of mer operons among bacteria. This was confirmed by a study that found that mer operons were distributed worldwide in Bacilli with dissemination of TnMERI1-like transposons. In this mini review, possible strategies for transposon-mediated in situ molecular breeding (ISMoB) of HgR bacteria in their natural habitat are discussed. In ISMoB, the target microorganisms for breeding are indigenous bacteria that are not HgR but that are dominant and robust in their respective environments. Additionally, we propose a new concept of bioremediation technology for environmental mercury pollution by applying transposon-mediated ISMoB for environmental mercury pollution control. - Takeshi Miki; Taichi Yokokawa; Po-Ju Ke; I-Fang Hsieh; Chih-hao Hsieh; Tomonori Kume; Kinuyo Yoneya; Kazuaki MatsuiEcological Research 33 1 249 - 260 2018年01月 [査読有り]
EcoPlate quantifies the ability of a microbial community to utilize 31 distinct carbon substrates, by monitoring color development of microplate wells during incubation. Well color patterns represent metabolic profiles. Previous studies typically used color patterns representing average values of three technical replicates on the final day of the incubation and did not consider substrate chemical diversity. However, color fluctuates during incubation and color varies between replicates, undermining statistical power to distinguish differences among samples in microbial functional composition and diversity. Therefore, we developed a protocol to improve statistical power with two approaches. First, we optimized data treatment for color development during incubation and technical replicates. Second, we incorporated chemical structural information for the 31 carbon substrates into the computation. Our framework implemented as the protocol in the R environment is able to compare the statistical power among different calculation methods. When we applied it to data from aquatic microcosm and forest soil systems, we observed substantial improvement in statistical power when we incorporated temporal patterns during incubation instead of using only endpoint data. Using maximum or minimum values of technical replicates also sometimes gave better results than averages. Incorporating chemical structural information based on fuzzy set theory could improve statistical power but only when relative color density information was considered it was not seen when the pattern was first binarized into the presence or absence of metabolic activity. Finally, we discuss research directions to improve these approaches and offer some practical considerations for applying our methods to other datasets. - 松井一彰; 横山雄一; 亀井訓平; 中口譲; 江口充; 谷口亮人; 竹原幸生; 麓隆行土木学会論文集G(環境) 73 3 134 - 142 公益社団法人 土木学会 2017年 [査読有り]
合流式下水道越流水（Combined Sewer Overflow: CSO）が都市河川に及ぼす影響と，大規模雨水貯留管（平成の太閤下水）の供用による改善効果の評価を目的に，降雨時の東横堀川（大阪市）にて細菌叢を指標とした水質調査を実施した．東堀橋地点では，4.0 mm/hを越える降雨時にCSOが原因と思われる懸濁態物質濃度の上昇と，それに伴う細菌叢の変化が確認された．しかしこのとき確認された河川中の細菌叢は，下水処理場で入手した処理前下水の細菌叢とは異なるものであった．雨水貯留管供用後も降雨時に河川水の電気伝導度が増加したが，それに伴った河川中の細菌叢の変化は確認されなかった．雨水貯留管の供用は，CSO流入を抑制することで，河川水質の改善や河川微生物群集攪乱の緩和に効果があると考えられた． - 松井一彰; 横川太一; 上田匡邦; 道越祐一; 水口亜樹; 松田裕之; 三木健日本生態学会誌 66 2 325 - 335 2016年 [査読有り]
- Kazuaki Matsui; Satoshi Yoshinami; Masaru Narita; Mei-Fang Chien; Le T. Phung; Simon Silver; Ginro EndoFEMS MICROBIOLOGY LETTERS 363 5 fnw013 2016年03月 [査読有り]
A total of 65 spore-forming mercury-resistant bacteria were isolated from natural environments worldwide in order to understand the acquisition of additional genes by and dissemination of mercury resistance transposons across related Bacilli genera by horizontal gene movement. PCR amplification using a single primer complementary to the inverted repeat sequence of TnMERI1-like transposons showed that 12 of 65 isolates had a transposon-like structure. There were four types of amplified fragments: Tn5084, Tn5085, Tn(d)MER3 (a newly identified deleted transposon-like fragment) and Tn6294 (a newly identified transposon). Tn(d)MER3 is a 3.5-kb sequence that carries a merRETPA operon with no merB or transposase genes. It is related to the mer operon of Bacillus licheniformis strain FA6-12 from Russia. DNA homology analysis shows that Tn6294 is an 8.5-kb sequence that is possibly derived from Tn(d)MER3 by integration of a TnMERI1-type transposase and resolvase genes and in addition the merR2 and merB1 genes. Bacteria harboring Tn6294 exhibited broad-spectrum mercury resistance to organomercurial compounds, although Tn6294 had only merB1 and did not have the merB2 and merB3 sequences for organomercurial lyases found in Tn5084 of B. cereus strain RC607. Strains with Tn6294 encode mercuric reductase (MerA) of less than 600 amino acids in length with a single N-terminal mercury-binding domain, whereas MerA encoded by strains MB1 and RC607 has two tandem domains. Thus, Tn(d)MER3 and Tn6294 are shorter prototypes for TnMERI1-like transposons. Identification of Tn6294 in Bacillus sp. from Taiwan and in Paenibacillus sp. from Antarctica indicates the wide horizontal dissemination of TnMERI1-like transposons across bacterial species and geographical barriers. - Takeshi Miki; Taichi Yokokawa; Kazuaki MatsuiPROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 281 1776 20132498 2014年02月 [査読有り]
Ecosystems have a limited buffering capacity of multiple ecosystem functions against biodiversity loss (i.e. low multifunctional redundancy). We developed a novel theoretical approach to evaluate multifunctional redundancy in a microbial community using the microbial genome database (MBGD) for comparative analysis. In order to fully implement functional information, we defined orthologue richness in a community, each of which is a functionally conservative evolutionary unit in genomes, as an index of community multifunctionality (MF). We constructed a graph of expected orthologue richness in a community (MF) as a function of species richness (SR), fit the power function to SR (i.e. MF = cSR(a)), and interpreted the higher exponent a as the lower multifunctional redundancy. Through a microcosm experiment, we confirmed that MF defined by orthologue richness could predict the actual multiple functions. We simulated random and non-random community assemblages using full genomic data of 478 prokaryotic species in the MBGD, and determined that the exponent in microbial communities ranged from 0.55 to 0.75. This exponent range provided a quantitative estimate that a 6.6-8.9% loss limit in SR occurred in a microbial community for an MF reduction no greater than 5%, suggesting a non-negligible initial loss effect of microbial diversity on MF. - Zen'ichiro Kawabata; Toshifumi Minamoto; Mie N. Honjo; Kimiko Uchii; Hiroki Yamanaka; Alata A. Suzuki; Yukihiro Kohmatsu; Kota Asano; Tomoaki Itayama; Tomoaki Ichijo; Koji Omori; Noboru Okuda; Masayuki Kakehashi; Masao Nasu; Kazuaki Matsui; Masatomi Matsuoka; Hainan Kong; Teruhiko Takahara; Deyi Wu; Ryuji YonekuraECOLOGICAL RESEARCH 26 6 1011 - 1016 2011年11月 [査読有り]
To predict outbreaks of infectious disease and to prevent epidemics, it is essential not only to conduct pathological studies but also to understand the interactions between the environment, pathogen, host and humans that cause and spread infectious diseases. Outbreaks of mass mortality in carp caused by Cyprinid herpesvirus 3 (CyHV-3), formerly known as koi herpesvirus (KHV), disease have occurred worldwide since the late 1990s. We proposed an environment-KHV-carp-human linkage as a conceptual model for "environmental diseases" and specify research subjects that might be necessary to construct and shape this linkage. - Transmission dynamics of an emerging infectious disease in wildlife through host reproductive cyclesKimiko Uchii; Arndt Telschow; Toshifumi Minamoto; Hiroki Yamanaka; Mie N. Honjo; Kazuaki Matsui; Zen'ichiro KawabataISME JOURNAL 5 2 244 - 251 2011年02月 [査読有り]
Emerging infectious diseases are major threats to wildlife populations. To enhance our understanding of the dynamics of these diseases, we investigated how host reproductive behavior and seasonal temperature variation drive transmission of infections among wild hosts, using the model system of cyprinid herpesvirus 3 (CyHV-3) disease in common carp. Our main findings were as follows: (1) a seroprevalence survey showed that CyHV-3 infection occurred mostly in adult hosts, (2) a quantitative assay for CyHV-3 in a host population demonstrated that CyHV-3 was most abundant in the spring when host reproduction occurred and water temperature increased simultaneously and (3) an analysis of the dynamics of CyHV-3 in water revealed that CyHV-3 concentration increased markedly in breeding habitats during host group mating. These results indicate that breeding habitats can become hot spots for transmission of infectious diseases if hosts aggregate for mating and the activation of pathogens occurs during the host breeding season. The ISME Journal (2011) 5, 244-251; doi:10.1038/ismej.2010.123; published online 26 August 2010 - Mei-Fang Chien; Masaru Narita; Kuo-Hsing Lin; Kazuaki Matsui; Chieh-Chen Huang; Ginro EndoJOURNAL OF BIOSCIENCE AND BIOENGINEERING 110 1 94 - 98 2010年07月 [査読有り]
Organomercury lyase (MerB) is a key enzyme in bacterial detoxification and bioremediation of organomercurials. However, the merB gene is often considered as an ancillary component of the mer operon because there is zero to three merB genes in different mer operons identified so far. In this study, organomercurials' removal abilities of native mercury-resistant bacteria that have one or multiple merB genes were examined. Each heterogeneous merB genes from these bacteria was further cloned into Escherichia coli to investigate the substrate specificity of each MerB enzyme. The merB1 gene from Bacillus megaterium MB1 conferred the highest volatilization ability to methylmercury chloride, ethylmercury chloride, thimerosal and p-chloromercuribenzoate, while the merB3 from B. megaterium MB1 conferred the fastest mercury volatilization activity to p-chloromercuribenzoate. The substrate specificities among these MerB enzymes show the necessity for selecting the appropriate bacteria strains or MerB enzymes to apply them in bioremediation engineering for cleaning up specific organomercurial contaminations. (C) 2010, The Society for Biotechnology, Japan. All rights reserved. - Mie N. Honjo; Toshifumi Minamoto; Kazuaki Matsui; Kimiko Uchii; Hiroki Yamanaka; Alata A. Suzuki; Yukihiro Kohmatsu; Takaji Iida; Zen'ichiro KawabataAPPLIED AND ENVIRONMENTAL MICROBIOLOGY 76 1 161 - 168 2010年01月 [査読有り]
Cyprinid herpesvirus 3 (CyHV-3), a lethal DNA virus that spreads in natural lakes and rivers, infects common carp and koi. We established a quantification method for CyHV-3 that includes a viral concentration method and quantitative PCR combined with an external standard virus. Viral concentration methods were compared using the cation-coated filter and ultrafiltration methods. The recovery of virus-like particles was similar for the two methods (cation-coated filter method, 44% +/- 19%, n = 3; ultrafiltration method, 50% +/- 3%, n = 3); however, the former method was faster and more suitable for routine determinations. The recovery of seeded CyHV-3 based on the cation-coated filter method varied by more than 3 orders of magnitude among the water samples. The recovery yield of CyHV-3 was significantly correlated with that of the seeded lambda phage, and the average ratio of lambda to the CyHV-3 recovery yield was 1.4, indicating that lambda is useful as an external standard virus for determining the recovery yield of CyHV-3. Therefore, to quantify CyHV-3 in environmental water, a known amount of lambda was added as an external standard virus to each water sample. Using this method, CyHV-3 DNA was detected in 6 of the 10 (60%) types of environmental water tested; the highest concentration of CyHV-3 DNA was 2 x 10(5) copies liter(-1). The lowest recovery limit of CyHV-3 DNA was 60 copies liter(-1). This method is practical for monitoring CyHV-3 abundance in environmental water. - K. Uchii; K. Matsui; T. Iida; Z. KawabataJOURNAL OF FISH DISEASES 32 10 857 - 864 2009年10月 [査読有り]
Cyprinid herpesvirus 3 (CyHV-3), which causes a lethal disease in common carp, Cyprinus carpio L., and koi, C. carpio koi, first occurred in Lake Biwa, Japan in 2004. To elucidate distribution of CyHV-3 in a wild common carp population, we conducted a PCR survey of CyHV-3 among such fish in Lake Biwa in 2006. Only 6% (1/18) of the common carp smaller than 300 mm were positive with PCR, whereas 31% (18/58) of fish larger than 300 mm were positive. To evaluate their past exposure to CyHV-3 infection based on the presence of antibodies, we also measured the levels of serum anti-CyHV-3 antibodies in the carp, using an enzyme-linked immunosorbent assay. None (0/26) of the fish smaller than 300 mm was positive for the antibodies, whereas 54% (33/61) of fish larger than 300 mm were positive. Of the antibody-positive individuals, 44% (14/32) were also positive by PCR strongly suggesting that wild common carp that survived infection become CyHV-3 carriers. Five individuals were positive by PCR but negative for antibodies indicating that their infection with CyHV-3 had occurred recently. These results suggest that transmission of CyHV-3 from carriers to naive common carp is still occurring in Lake Biwa. - Ryuji Yonekura; Hiroki Yamanaka; Atushi Ushimaru; Kazuaki Matsui; Kimiko Uchii; Atsushi MaruyamaBIOLOGICAL INVASIONS 11 6 1347 - 1355 2009年06月 [査読有り]
Asymmetry in the competition abilities between invasive and native consumers can potentially influence the colonization success by invasive species. We tested whether a subsidy of allochthonous prey enhanced an asymmetric competition between invasive bluegill (Lepomis macrochirus) and two native cyprinid fish, that is, stone moroko (Pseudorasbora parva) and tamoroko (Gnathopogon elongatus elongatus). A field experiment was conducted using enclosures wherein the strength of interspecific competition and the presence/absence of allochthonous prey were manipulated. The experiment revealed that allochthonous prey alleviated the limitation of fish growths caused by a severe competition for aquatic prey resources. However, the importance of allochthonous prey differed considerably between invasive bluegill and the two native cyprinids. Individual bluegills grew faster when the allochthonous prey was supplied, whereas no difference in growth was observed in the two cyprinids whether or not allochthonous prey was supplied. Interestingly, the importance of allochthonous prey on the total amount of bluegill growth varied depending on the numerical abundance of native cyprinid competitors, and this importance increased when the native cyprinids were abundant. These findings indicated that allochthonous prey provides an asymmetric growth benefit to invasive bluegills over the two native cyprinids by alleviating asymmetrically the competition strength in a Japanese pond, especially under the conditions of severe interspecific resource competition and a limitation in the utilization of in situ prey resources. - Kazuaki Matsui; Mie Honjo; Yukihiro Kohmatsu; Kimiko Uchii; Ryuji Yonekura; Zen'ichiro KawabataFRESHWATER BIOLOGY 53 6 1262 - 1272 2008年06月 [査読有り]
1. Koi herpesvirus (KHV) disease, a lethal disease in carp and koi, has spread rapidly since the late 1990s. Only a few studies have focused on the ecology of KHV in natural freshwater environments, although characterization and the diagnosis of KHV disease have been intensively studied. This is mainly due to the lack of reliable detection methods for KHV in natural environments. 2. Three different approaches have been used to detect KHV in fish samples: (i) isolation and identification of the virus using cultured cell lines; (ii) detection of viral DNA with target DNA amplification techniques; and (iii) detection of anti-KHV antibodies using an enzyme-linked immunosorbent assay (ELISA). Among these methods, DNA amplification techniques have become most popular because of their sensitivity and simplicity. 3. In contrast to KHV detection in fish samples, detection of KHV from water and sediment samples has not been reported. This is the result of a lack of reliable KHV concentration methods for water and sediment samples, although methodology from public health and marine microbiology may be applicable to determine KHV concentration in such samples. 4. Few reports have evaluated the environmental factors (e.g. temperature, UV irradiation) that affect the fate of KHV in water bodies. Mass mortality of wild carp caused by KHV infection would result in the removal of a large omnivorous fish from freshwater ecosystems and may thus alter community structure and ecosystem functioning. - Kimiko Uchii; Noboru Okuda; Ryuji Yonekura; Zin'ichi Karube; Kazuaki Matsui; Zen'ichiro KawabataLIMNOLOGY 8 1 59 - 63 2007年04月 [査読有り]
Trophic polymorphism was recently reported in introduced bluegill (Lepomis macrochirus) in Lake Biwa, Japan, where three morphs are specialized in benthic invertebrates (benthivorous type), submerged aquatic plants (herbivorous type), and zooplankton (planktivorous type). We evaluated the long-term effects of food resource utilization by these trophic morphs using stable isotope ratios, delta N-15 and delta C-13. A significant difference in delta N-15 was found between the benthivorous and planktivorous types. The planktivorous type had the higher delta N-15 value, which corresponded with the value expected from its prey, zooplankton. The lower delta N-15 value of the benthivorous type would be derived from the lower delta N-15 values of benthic prey organisms compared to zooplankton. These results support previous findings that the benthivorous and planktivorous types have different food resource utilization. In contrast, the delta N-15 and delta C-13 values of the herbivorous type were distinctly different from the expected values, indicating that this type vas unlikely to utilize aquatic plants substantially, contradicting the results of the dietary analysis. - 松井一彰; 成田勝; 遠藤銀朗日本生態学会誌 57 3 390 - 397 一般社団法人 日本生態学会 2007年 [査読有り]
水銀は生物に対して高い毒性作用を示す有害重金属である。しかし水銀耐性遺伝子を持つ細菌は、水銀を様々な形態に変換させる事によってその毒性作用を回避する事ができる。このような細菌による水銀の形態変換は、水銀の地球規模での循環にも深く関与していると考えられているが、地球上における水銀耐性細菌および水銀耐性遺伝子の分布・分散様式についてはほとんどわかっていない。近年、砂塵に付着し左細菌は、数千キロメートルという距離を超えて移動・拡散している事が明らかになってきた。また原核生物である細菌は、個体間で遺伝子を授受する「遺伝子の水平伝播」によって同種内における遺伝子の多様さを産み出していると考えられている。本稿では、地球規模で細菌細胞および遺伝子が移動・拡散している事を追跡するための指標として、Bacillus属細菌がもつ水銀耐性遺伝子を指標にした研究例を紹介する。これまでに発見されているBacillus属細菌がもつ水銀耐性遺伝子の多くは、TnMERI1型の水銀耐性トランスポゾンの構造を取っている物が多い。そこでこのTnMERI1型トランスポゾンとその外側にみられるDR (Direct repeat)配列を基に、細菌細胞と水銀耐性遺伝子の分散について検証したところ、(1)水銀耐性遺伝子が細菌種間で水平伝播されており、遺伝子の分散が起こっている事、(2)同一の遺伝子を持つ同属同種の細菌細胞が地球規模で分布している事がわかってきた。今後は目に見える生物を対象に研究されてきた生態学分野の知見も取り入れ、微生物世界の分布・分散についての理解を深めていく必要があると思われる。 - 松井一彰; 遊佐清孝; 菅原宏幸; 成田勝; 遠藤銀朗水環境学会誌 30 2 77 - 81 Japan Society on Water Environment 2007年 [査読有り]
A whole-cell bacterial biosensor was developed for detecting low concentrations of organomercurials in the environment. The sensor plasmid for bioavailable Hg2+ (pHYRnLux) was constructed by fusing the mercury inducible promoter and its regulatory gene, merR, from Pseudomonas strain K-62 with the reporter genes luxAB from Vibrio harveyi. Another plasmid carrying the organomercurial lyase gene, merB3, from Bacillus megaterium MB1 was also constructed and transformed into Escherichia coli DH5 α with pHYRnLux. The resulting bacterial biosensor carrying these two plasmids responded to a various organomercurials including methylmercury chloride (MMC), ethylmercury chloride (EMC), phenylmercury acetate (PMA) and thimerosal. Under a 10-minute exposure condition, the lowest detectable concentrations of MMC and PMA were 3nM and 30nM, respectively. However, increasing the exposure time to 60 minutes allowed the detection of 300pM MMC and 3nM PMA. Thus, the constructed bacterial biosensor allows the detection of a various organomercurials from the picomolar level to the nanomolar level within 60 minutes and may be useful for monitoring bioavailable organomercurials in environmental samples. - Mie Honjo; Kazuaki Matsui; Nobuyoshi Ishii; Masami Nakanishi; Zen'ichiro KawabataFUNDAMENTAL AND APPLIED LIMNOLOGY 168 1 105 - 112 2007年 [査読有り]
We investigated the vertical distribution and dynamics of viral abundance in the euphotic epilimnion and aphotic hypolimnion during periods of stratification in Lake Biwa, Japan. The abundance of virus-like particles (VLPs) was lower and more constant in the hypolimnion than in the epilimnion. Simple correlation analysis demonstrated that during periods of stratification, VLP abundance in the hypolimnion was positively correlated with chlorophyll-a concentration, the bacterial abundance of the >0.8-mu m fraction, and water temperature. On the other hand, VLP abundance in the epilimnion was also positively correlated with chlorophyll-a concentration and the bacterial abundance of the >0.8-mu m fraction, but was negatively correlated with the bacterial abundance of the 0.2-0.8-mu m fraction and was not correlated with water temperature. We observed no correlation between VLP abundance and total bacterial abundance in either the epilimnion or the hypolimnion. Multiple regression analysis indicated that VLP abundance was explained by chlorophyll-a concentration in both the epilimnion and the hypolimnion. Aphotic environment and abundance of phytoplankton and bacteria in the hypolimnion, suggesting that main host of viruses is bacteria rather than phytoplankton. However our results suggest that VLP abundance in the hypolimnion related to the other factors as well as bacterial abundance. - 三木健; 松井一彰; 横川太一; 西田貴明; 小林由紀; 内井喜美子日本生態学会誌 57 3 424 - 431 一般社団法人 日本生態学会 2007年 [査読有り]
本特集では、微生物群集の構造やその群集が持つ機能の、時空間的不均一性に注目した話題を提供してきた。さまざまな空間スケールでの微生物群集の空間分布と物質循環の関係についての4つの話題と、共生微生物と宿主間の柔軟で可塑的な相互作用に関する2つの話題について、それぞれ整理する。それを受けて、自由生活微生物と環境の関係と、共生微生物と宿主の関係という一見関連性の低い事象を統一的に理解するために「マイクロビアル・プール」という概念を提案する。地球上のすべての微生物およびその遺伝子の集合体として定義されるマイクロビアル・プールが、生態系の中でどのような役割を担っているのかを、注目する時間および空間スケールに応じて分類する。最後に、マイクロビアル・プールがいかに地球上の生物多様性に影響を与えうるかを論じたい。 - K Uchii; K Matsui; R Yonekura; K Tani; T Kenzaka; M Nasu; Z KawabataMICROBIAL ECOLOGY 51 3 277 - 284 2006年04月 [査読有り]
Bluegill (Lepomis macrochirus) in Lake Biwa, Japan, feed on benthic invertebrates (benthivorous type), aquatic plants (herbivorous type), and zooplankton (planktivorous type). To evaluate the effect of food on intestinal bacterial microbiota, we characterized and compared the intestinal microbiota of these three types of bluegill in terms of community-level physiological profile (CLPP) and genetic structure. The CLPP was analyzed using Biolog MicroPlates (Biolog, Inc., Hayward, CA, USA), and multivariate analysis of variance revealed that the CLPP of intestinal microbiota differed significantly between any pairs of the three types of bluegill. The genetic profiles were analyzed by temperature gradient gel electrophoresis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments, and multidimensional scaling indicated the existence of specific intestinal bacterial structures for both the benthivorous and the planktivorous types. These results suggest that the host's feeding habit can be one factor controlling the intestinal microbiota of fish in the natural environment. - M Honjo; K Matsui; M Ueki; R Nakamura; JA Fuhrman; Z KawabataJOURNAL OF PLANKTON RESEARCH 28 4 407 - 412 2006年04月 [査読有り]
We demonstrated the existence of the diversity of virus-like agents killing Microcystis aeruginosa in a shallow hyper-eutrophic pond in Japan, but without specific virus isolation, once in October and twice in December 2001. The pond water was treated by chloroform, filtered throughout GF-75 filter (0.3 mu m) and then incubated with axenic M. aeruginosa for 7 days. The cell density of M. aeruginosa decreased 10-fold in the course of the incubation. Results suggested that the active agent for cell death was virus-like, based upon its size fraction (from 30 kDa to 0.3 mu m), sensitivity on heat-treatment, and evidence of protein protection of DNA during our extraction procedure. From our results of pulsed field gel electrophoresis (PFGE) analysis, two or four different-sized DNA bands appeared from the culture lysate in each experiment. In total, 10 different-sized bands (10-90 kb) were observed from the three sample dates, suggesting that multiple virus-like agents killing M. aeruginosa existed in this pond. To our knowledge, this is the first report of the existence of virus-like agents with different genomic size that kill M. aeruginosa. - 遠藤銀朗; 松井一彰; 成田勝環境バイオテクノロジー学会誌 61 1 27 - 32 環境バイオテクノロジー学会 2006年 [査読有り]
- K Matsui; M Narita; H Ishii; G EndoFEMS MICROBIOLOGY LETTERS 253 2 309 - 314 2005年12月 [査読有り]
As an initial step to understand the mobile nature of class II mercury resistance transposon TnMERI1, the effect of the recA gene on translocation of mini-TnMERI1 was evaluated. A higher transposition frequency in the LE392 strain (2.4 +/- 1.2 x 10(-5)) than in the recA-deficient DH1 strain (1.2 +/- 0.8 x 10(-6)) indicated participation of the recA gene in mini-TnMERI1 transposition. Introduction of the recA gene into the DH1 strain complemented the transposition frequency at the same level as in LE392 and confirmed participation of the recA gene in transposition. However, treatment of cells by stress agents, including irradiation of up to 3000 J m(-2) UV doses, did not alter the transposition frequency and suggested independence of RecA from the SOS stress response. Further analysis of transconjugants indicated participation of RecA in the resolution of the cointegrate structure of the transposon. These results suggested that RecA is a constitutive cellular factor that increases translocation of mini-TnMERI1 and may participate in dissemination of TnMERI1-like transposons. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. - M Ueki; K Matsui; KS Choi; Z KawabataFEMS MICROBIOLOGY ECOLOGY 51 1 1 - 8 2004年12月 [査読有り]
Conjugal plasmid transfer from Escherichia coli S17-1 (pBHR1) to Pseudomonas stutzeri was investigated in the presence of a cyanophyta Microcystis aeruginosa. The plasmid transfer frequency increased with higher densities or ill. aeruginosa. The extracellular metabolic products (EMPs) from M. aeruginosa were found to enhance the plasmid transfer between bacteria. Furthermore. the plasmid transfer frequency in medium containing EMI's was significantly higher than that in culture medium with or without glucose. These results suggest that M. aeruginosa enhances conjugal plasmid transfer between bacteria through its EMPS, and that identity of the carbon Source is ail important factor affecting conjugal plasmid transfer in aquatic environments. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. - N Ishii; K Matsui; S Fuma; H Takeda; Z KawabataFEMS MICROBIOLOGY LETTERS 240 2 151 - 154 2004年11月 [査読有り]
We studied the transforming ability of the extracellular plasmid DNA released from a genetically engineered Escherichia coli pEGFP and the culturing conditions for the release of transforming DNA. The transforming ability was evaluated by transformation of competent cells with filtrates of E. coli pEGFP cultures. The number of transformants increased with time when E. coli pEGFP cells grew exponentially in rich medium, but not in stationary phase or when inoculated in freshwater. These results suggested that crude extracellular plasmid DNA had transforming ability and this transforming DNA was mainly released by actively growing bacteria. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. - K Matsui; N Ishii; M Honjo; Z KawabataAQUATIC MICROBIAL ECOLOGY 36 1 99 - 105 2004年06月 [査読有り]
A rapid fluorometric assay using the fluorescent dye SYBR Green I was established to determine the concentration of dissolved nucleic acids in fresh water. The sensitivity of SYBR Green I to double stranded DNA (dsDNA) (lambdaHind III digest) was as low as 50 pg, 200 times more sensitive than Hoechst 33258. SYBR Green I bound to dsDNA emitted 10 times stronger fluorescence than when bound to single stranded DNA or RNA, indicating its selectivity for dsDNA measurement. The dissolved DNA (dDNA) concentration in fresh water determined using SYBR Green I was almost the same as that obtained using Hoechst 33258. This suggests that the dDNA measured by SYBR Green I is comparable to that determined by Hoechst 33258 in previous studies. To reduce preparation time, the dDNA in lake water filtrate was precipitated by ethanol and purified using a centrifugal filter device. The overall preparation process takes only a few hours and requires only 10 ml of water. The process described here may, therefore, facilitate 'same day' measurement of dDNA dynamics in freshwater environments. - M Narita; K Matsui; CC Huang; Z Kawabata; G EndoFEMS MICROBIOLOGY ECOLOGY 48 1 47 - 55 2004年04月 [査読有り]
Fifty-six mercury-resistant (Hg-R) Bacillus strains were isolated from natural environments at various sites of the world. Southern hybridisation and polymerase chain reaction (PCR) analysis showed that 21 of the 56 isolates have closely related or identical mer operons to that of Bacillus megaterium MB1. These 21 isolates displayed a broad-spectrum mercury resistance and volatilised Hg-0. PCR amplification with a single primer and restriction fragment length polymorphism analysis showed that these 21 isolates had TnMEII-like class II transposons. These transposons can be classified into Tn5084, Tn5085, or TnMER11. From these results, at least three types of class II mercury resistance transposons exist in Hg-R Bacillus and these transposons may contribute the worldwide distribution and horizontal dissemination of the mer operons among Bacillus strains in natural environments. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. - K Matsui; N Ishii; Z KawabataFEMS MICROBIOLOGY ECOLOGY 45 3 211 - 218 2003年08月 [査読有り]
The involvement of microbial interactions in natural transformation of bacteria was evaluated using an aquatic model system. For this purpose, the naturally transformable Bacillus subtilis was used as the model bacterium which was co-cultivated with the protist Tetrahymena thermophila (a consumer) and/or the photosynthetic alga Euglena gracilis (a producer). Co-cultivation with as few as 10(2) individuals ml(-1) of T. thermophila lowered the number of transformants to less than the detectable level (< 1 X 10(0) ml(-1)), while co-cultivation with E. gracilis did not. Metabolites from co-cultures of T. thermophila and B. subtilis also decreased the number of transformants to less than the detectable level, while metabolites from co-culture of T. thermophila and B. subtilis with E. gracilis did not. Thus, the introduction of transformation inhibitory factor(s) by the grazing of T. thermophila and the attenuation of this inhibitory factor(s) by E. gracilis is indicated. These observations suggest that biological components do affect the natural transformation of B. subtilis. The study described is the first to suggest that ecological interactions are responsible not only for the carbon and energy cycles, but also for the processes governing horizontal transfer of genes, in microbial ecosystems. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. - K Matsui; N Ishii; Z KawabataAPPLIED AND ENVIRONMENTAL MICROBIOLOGY 69 4 2399 - 2404 2003年04月 [査読有り]
We studied the effects of cocultivation with either Euglena gracilis (Euglenophyta), Microeystis aeruginosa (Cyanophyta), Chlamydomonas neglecta (Chlorophyta), or Carteria inversa (Chlorophyta) on the production of extracellular plasmid DNA by Escherichia coli LE392(pKZ105). Dot,blot hybridization analysis showed a significant release of plasmid DNA by cocultivation with all the algae tested. Further analysis by electrotransformation confirmed the release of transformable plasmid DNA by cocultivation with either E. gracilis, M. aeruginosa, or C. inversa. These results suggest algal involvement in bacterial horizontal gene transfer by stimulating the release of transformable DNA into aquatic environments. - K Matsui; MS Jun; M Ueki; Z KawabataECOLOGICAL RESEARCH 16 5 905 - 912 2001年12月 [査読有り]
Changes of bacterioplankton diversity in take water were followed in triplicate, continuous-flow experimental tanks. Most probable numbers (MPN) were obtained for 95 different carbon sources using BIOLOG plates and were used to characterize bacterioptankton diversity. During 70 days of incubation, MPN declined for 15 of the 95 substrates while three of 95 appeared to be newly used, indicating functional succession in the bacterioplankton. Total bacterial cell abundance was constant from day 7 to day 70 of the incubation period. The succession of species composition of phyto- and zooplankton was also observed and suggested some involvement by phyto- and zooplankton species in the changes of bacterioplankton diversity. Thus, BIOLOG-based MPN assays is a simple but sensitive method for characterizing the changes in the bacterioplankton carbon utilization profile and is also useful for tracing the functional succession of bacterioplankton diversity within a community. - K Matsui; M Honjo; Z KawabataAQUATIC MICROBIAL ECOLOGY 26 1 95 - 102 2001年10月 [査読有り]
The fate of the exogenous plasmid DNA, pEGFP, was traced in lake waters collected from the epilimnion and the hypolimnion during a period of marked thermal stratification. Both gel electrophoresis analysis and dot-blot hybridization analysis demonstrated that the seeded pEGFP was completely degraded in epilimnion water within 170 h of incubation, while no pEGFP degradation was observed in hypolimnion water, Removal of microorganisms from the lake waters of the epilimnion and hypolimnion attenuated the degradation of pEGFP but did not stop it. Addition of EDTA to the lake water samples resulted in complete termination of the pEGFP degradation and indicated the involvement of a dissolved enzyme for DNA degradation, A measurable release of phosphate during pEGFP degradation indicated the existence of DNA-mediated phosphate regeneration in epilimnion water. In contrast, no DNA degradation in hypolimnion water suggested the possibility that it is less nutritionally important in the hypolimnion, Transformability of the pEGFP was not altered in hypolimnion water during the incubation, which suggested stability of genetic information that gradually decreased in epilimnion water. Dissolved DNA (dDNA) is commonly observed in aquatic environments and is thought to be an important nutritional source for microorganisms. However, our results suggest that dDNA in the hypolimnion environment may not be so important as a nutritional source for microbes, but might be more important as a genetic pool for further natural transformation. - K Matsui; N Ishii; Z KawabataBULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 66 2 139 - 145 2001年02月 [査読有り]
- K Matsui; S Kono; A Saeki; N Ishii; MG Min; Z KawabataHYDROBIOLOGIA 435 1-3 109 - 116 2000年09月 [査読有り]
Mechanisms for coexistence among micro-organisms were studied by using a species-defined microcosm, consisting of the bacterium Escherichia coli, the ciliate Tetrahymena thermophila and the alga Euglena gracilis. These organisms were chosen as representative of ecological functional groups i.e. decomposer, consumer and producer, respectively. Direct and indirect interactions among these organisms were evaluated by comparisons of their population dynamics in culture with different combinations of the three species. There was an E. coli cell density dependent predator-prey interaction between T. thermophila and E. coli which was only established when there were more than 10(6) cells ml(-1) of E. coli. Indirect interactions were evaluated from the cultivation of each organism in media containing metabolites of the others. Metabolites from each population strongly accelerated the growth of their own populations and those of the others except for the self-toxicity effect of E. coli metabolites. These observations suggested that not only the cell-cell contact of direct interactions, but also metabolite-mediated indirect interactions supported the maintenance of the populations of each micro-organism and their coexistence. In natural ecosystems, there are many interactions and it is difficult to evaluate all those regulating community dynamics. The gnotobiotic microcosm used in this study was shown to be suitable for examining the specific, species-species microbial interactions. - A Gupta; K Matsui; JF Lo; S SilverNATURE MEDICINE 5 2 183 - 188 1999年02月 [査読有り]
Here we report the genetic and proposed molecular basis for silver resistance in pathogenic microorganisms. The silver resistance determinant from a hospital burn ward Salmonella plasmid contains nine open reading frames, arranged in three measured and divergently transcribed RNAs. The resistance determinant encodes a periplasmic silver-specific binding protein (SilE) plus apparently two parallel efflux pumps: one, a P-type ATPase (SilP); the other, a membrane potential-dependent three-polypeptide cation/proton antiporter (SilCBA). The sil determinant is governed by a two-component membrane sensor and transcriptional responder comprising silS and silR, which are co-transcribed. The availability of the sil silver-resistance determinant will be the basis for mechanistic molecular and biochemical studies as well as molecular epidemiology of silver resistance in clinical settings in which silver is used as a biocide. - Silver, S; A. Gupta; K. Matsui; J. F. LoMetal-Based Drugs 6 315 - 320 1999年 [査読有り]
- Zen'ichiro Kawabata; Man-Gi Min; Kazuaki Matsui; Nobuyoshi IshiiInternational Journal of Environmental Studies 55 1-2 77 - 85 1998年 [査読有り]
In order to clarify some of the factors affecting the survival of a genetically engineered Escherichia coli (g-E. coli) bearing a plasmid in a paddy field microcosm and its parent E. coli (p-E. coli), we focused on protozoa and the metabolites of indigenous bacteria. It was found that both g-E. coli and p-E. coli decreased remarkably in a microcosm with protozoa but not so in that without protozoa. The populations of both g-E. coli and p-E. coli decreased much more in the metabolites of indigenous bacteria than in those of their respective metabolites. Therefore, the metabolites of indigenous bacteria as well as protozoa are factors affecting the decreases of both g-E. coli and p-E. coli in a paddy field microcosm. - Man-Gi Min; Zen'Ichiro Kawabata; Kazuaki Matsui; Nobuyoshi IshiInternational Journal of Environmental Studies 52 1-2 75 - 88 1997年 [査読有り]
- Kawabata, Z; M-G. Min; K. Matsui; N. IshiiJapanese Journal of Water Treatment Biology 32 1 61 - 66 Japanese Society of Water Treatment Biology 1996年 [査読有り]
In order to count Escherichia coli both sensitive and resistant to ampicillin in a paddy field community, the quantity of Colilert, the time for incubation, and concentration of ampicillin were studied. Colilert was dissolved with sterilized distilled water and dispensed into a 1.5ml centrifuging tube and the MPN method under 30- to 70-hours incubation was adopted for enumeration of E. coli. Commercial Colilert could be reused 20 times. Colilert containing 0.5mg/l of ampicillin was applicable for counting E. coli resistant to ampicillin. - Z KAWABATA; K MATSUI; K OKAZAKI; M NASU; N NAKANO; T SUGAIJOURNAL OF PROTOZOOLOGY RESEARCH 5 1 23 - 26 1995年01月 [査読有り]
A species-defined microcosm, consisting of bacteria Escherichia coli DH5a, protozoa Tetrahymena thermophila B and algae Euglena gracilis Z, was synthesized in which all species co-existed for over 130 days. This microcosm was developed as an experimental system to study gene-population interaction in a community.
書籍
- 遺伝情報の動態：微生物の遺伝子水平伝播 (In 日本生態学会 編, シリーズ現代の生態学 第9巻「淡水生態学のフロンティア」)(担当:分担執筆範囲:)共立出版 2012年 ISBN: 9784320057371
- 微生物生態学入門, 日本微生物生態学会 教育研究部会 編薯日科技連出版 2004年 ISBN: 4817190671
- 水中を移動する遺伝子—遺伝情報の多様化に果たす細菌間の遺伝子伝播 (In 大串 隆之 編、生物多様性学のすすめ)丸善出版 2003年 ISBN: 4621072188
講演・口頭発表等
- 「微生物群集機能を評価するためのエコプレートの統計解析講座」 [通常講演]三木健; 横川太一; 松井 一彰ワークショップ@京都大学生態学研究センター 2017年11月 公開講演，セミナー，チュートリアル，講習，講義等
MISC
- Takeshi Miki; Masahiro Nakamura; Hiroko Kurokawa; Kazuaki Matsui Ecological Research 37 (4) 450 -454 2022年07月
- Takeshi Miki; Masahiro Nakamura; Kazuaki Matsui; Hazime Mizoguchi; Emi Tamaki Ecological Research 35 (4) 575 -578 2020年07月
- 松井一彰; 横川太一 日本生態学会誌 66 (2) 287 -289 2016年
- 三木健; 三木健; 松井一彰; 横川太一 日本微生物生態学会大会(Web) 30th 2015年
- 松井一彰; 三木健; 横川太一 日本土壌微生物学会講演要旨集 2014 2014年
- 松井 一彰; 吉浪 賢史; 成田 勝; 遠藤 銀朗 日本微生物生態学会講演要旨集 (28) 183 -183 2012年
- 吉浪 賢史; 成田 勝; 遠藤 銀朗; 松井 一彰 日本微生物生態学会講演要旨集 (26) 191 -191 2010年
- 松井 一彰; 本庄 三恵; 川端 善一郎; 内井 喜美子 日本微生物生態学会講演要旨集 (24) 92 -92 2008年
- 成田 勝; 簡 梅芳; 松井 一彰; 黄 介辰; 遠藤 銀朗 日本微生物生態学会講演要旨集 (24) 119 -119 2008年
- 内井喜美子; 米倉竜次; 松井一彰; 奥田昇; 川端善一郎 日本生態学会大会講演要旨集 54th 112 2007年03月
- 内井喜美子; 奥田昇; 米倉竜次; 松井一彰; 川端善一郎 日本生態学会大会講演要旨集 53rd 362 2006年03月
- 松井 一彰; 成田 勝; 川端 善一郎; 遠藤 銀朗 日本微生物生態学会講演要旨集 (22) 124 -124 2006年
- Trophic polymorphism in Lake Biwa bluegill, an introduced fish species in Japan: stable isotopic evidenceUchii, K; Okuda, N; Karube, Z; Yonekura, R; Matsui, K; Kawabata, Z The American Society of Limnology and Oceanography Summer Meeting 2006年
- 川端 善一郎; 松井 一彰; 石井 伸昌; 植木 昌也 日本微生物生態学会講演要旨集 (21) 298 -298 2005年
- 松井 一彰; 成田 勝; 遠藤 銀朗 日本微生物生態学会講演要旨集 (21) 177 -177 2005年
- 遠藤 銀朗; 成田 勝; 松井 一彰 日本生物工学会大会講演要旨集 17 84 -84 2005年
- 水俣湾底泥から分離されたBacillus属細菌由来のヒ素耐性トランスポゾンおよびそのarsオペロンの特徴に関する研究佐藤充則; 黄介辰; 成田勝; 松井一彰; 遠藤銀朗 東北学院大学環境防災工学研究所紀要 16 3 -8 2005年
- 松井 一彰; 成田 勝; 黄 介辰; 遠藤 銀朗 日本微生物生態学会講演要旨集 (20) 171 -171 2004年
- 内井 喜美子; 松井 一彰; 米倉 竜次; 谷 佳津治; 見坂 武彦; 那須 正夫; 川端 善一郎 日本微生物生態学会講演要旨集 (20) 140 -140 2004年
- 成田 勝; 松井 一彰; 黄 介辰; 遠藤 銀朗 日本微生物生態学会講演要旨集 (20) 68 -68 2004年
- 遠藤 銀朗; 佐藤 充則; 成田 勝; 松井 一彰 日本生物工学会大会講演要旨集 16 167 -167 2004年
- 成田 勝; 松井 一彰; 石井 秀学; 黄 介辰; 遠藤 銀郎 日本微生物生態学会講演要旨集 (19) 53 -53 2003年
- 松井 一彰; 石井 伸昌; 川端 善一郎 日本微生物生態学会講演要旨集 (19) 15 -15 2003年
- 石井 伸昌; 武田 洋; 松井 一彰; 府馬 正一; 宮本 霧子; 柳澤 啓; 川端 善一郎 日本微生物生態学会講演要旨集 (18) 22 -22 2002年
- 松井 一彰; 本庄 三恵; 川端 善一郎 日本微生物生態学会講演要旨集 (17) 43 -43 2001年
- 石井 伸昌; 松井 一彰; 武田 洋; 府馬 正一; 宮本 霧子; 柳澤 啓; 川端 善一郎 日本微生物生態学会講演要旨集 (16) 17 -17 2000年
共同研究・競争的資金等の研究課題
- 日本学術振興会：科学研究費助成事業研究期間 : 2022年04月 -2025年03月代表者 : 小林 由紀; 渡邉 健太; 度会 雅久; 松井 一彰
- 日本学術振興会：科学研究費助成事業 基盤研究(B)研究期間 : 2020年04月 -2023年03月代表者 : 松井 一彰1年目となる本年は、研究計画書にて提示した3つの研究ステップのうち、ステップ1「下水管内での微生物動態調査法の確立」と、ステップ2「微生物群集と薬剤耐性細菌の経時的な調査」を中心に研究を進めた。実施した内容は以下のとおりである。①異なるタイムスケールでの下水採水が実現できるよう、担当の自治体と運営会社の協力を得ながら、採水場所の確保と連絡体制や諸手続を含めた採水環境を整えた。②設備備品として申請していた自動採水器の運転調整を現場にておこない、年変動と日変動を追跡するための採水作業を開始した。③下水からDNAを回収する方法についての検討をおこない、確立した方法をマニュアル化することで、実験者間での差がでないように配慮した。マニュアル化した方法については米国の採水でも利用している。④公表されている学術論文を元にして指標となる薬剤耐性遺伝子の選別し、不純物混入が予想される下水サンプルを用いても、論文報告通りの遺伝子検出感度が得られることを検証した。当初予定していた薬剤耐性遺伝子のうち、期待していた感度が得られなかったターゲットについては、一旦対象リストから外す予定である。⑤細菌以外の真核微生物動態を追跡するために、共同研究者と次世代シーケンサーを用いた群集構造解析に着手した。⑥米国の研究協力者とともに、米国ウィスコンシン州における下水の経時的な採水を実施した。日本で確立した下水の採水および分析マニュアルを用いることで、米国での結果と日本での結果の比較検討が出来る予定である。
- 日本学術振興会：科学研究費助成事業 基盤研究(B)研究期間 : 2019年04月 -2022年03月代表者 : 三木 健; 鏡味 麻衣子; 松井 一彰植物プランクトンとツボカビの相互作用について個体間差異について、培養実験・タイムラプス撮影と数理モデリングを組み合わせて以下の３つを明らかにした。１）宿主細胞当たりの多重寄生数の個体間差異は宿主細胞とツボカビ遊走子との非ランダムな相互作用（すなわち局所的な密度依存性相互作用）から生じる。２）宿主細胞上の既存の寄生がさらなる寄生を促進し、宿主細胞あたりの新規遊走子生産数が寄生数に応じて増加する。３）これらの個体間差異が、平衡状態における寄生率の双安定性を生じさせ、過渡的状態における急激な感染流行拡大を引き起こす。
- 日本学術振興会：科学研究費助成事業 基盤研究(C)研究期間 : 2019年04月 -2022年03月代表者 : 小林 由紀; 松井 一彰近年，薬剤耐性菌の蔓延とともに，多剤耐性化した菌の出現も報告されており，世界的にも極めて深刻な問題となっている．院内環境やヒト体内において薬剤耐性菌に対する研究報告はされているものの，自然環境中における多剤耐性菌の動態についての知見は乏しい． そこで，本研究では真締川流域を調査地とし，グラム陰性桿菌を対象とした水環境中に存在する耐性菌を検出，相同性検索を目的とした．本研究では，マッコンキー寒天培地に発育し得るグラム陰性桿菌に主眼を置き検索した結果，多剤耐性菌（Pseudomonas属，Serratia属，Ochrobactrum属）や，Klebsiella属のESBL産生菌が検出された．
- 日本学術振興会：科学研究費助成事業 基盤研究(C)研究期間 : 2016年04月 -2019年03月代表者 : 松井 一彰; Silver Simon; 遠藤 銀朗微生物の空間分布については経験や観察を基にした仮説が存在するが、その検証は進めてこられなかった。本研究では、仮説の域を超えなかった細菌の地理的分布について、データを基にした検証を試みた。世界各地の土壌中よりバチラス属水銀耐性細菌を分離して水銀耐性トランスポゾンを解析したところ、同一のトランスポゾンを持つ同属の細菌が異なる地点間で見つかった。また同一のトランスポゾンが、別の属の細菌より見つかった事から、水銀耐性トランスポゾンがバチラス属細菌間で水平伝播されていることも示唆された。さらに水銀を含む培地での土壌培養によって、各地点の水銀耐性細菌が全て検出可能な量まで増加することを示した。
- 大阪湾沿岸を対象とした薬剤耐性細菌の分布と大阪市内河川からの影響に関する研究大阪湾圏域の海域環境再生・創造に関する研究助成制度研究期間 : 2018年07月 -2019年03月代表者 : 松井一彰
- 下水越流を通じて都市河川に流入する薬剤耐性遺伝子の動態解明河川財団研究助成金研究期間 : 2017年04月 -2018年03月代表者 : 松井一彰
- 雨水および越流下水が東横堀川・道頓堀川の微生物相に及ぼす影響の解析下水道振興基金研究助成金研究期間 : 2013年04月 -2016年03月代表者 : 松井 一彰
- 都市河川における微生物の多様性と水質浄化機構の解明 ー大阪道頓堀川を例としてーニッセイ財団研究助成研究期間 : 2012年04月 -2013年03月代表者 : 松井 一彰
- 日本学術振興会：科学研究費助成事業 基盤研究(C)研究期間 : 2008年 -2010年代表者 : 松井 一彰; 遠藤 銀朗遺伝子の水平伝播現象が細菌の進化や遺伝情報獲得に関与していることは古くから知られている。バクテリアの遺伝子水平伝播はプラスミドやトランスポゾンのような「可動性遺伝因子」を介しておこなわれるが、その伝達頻度や遺伝子の地理的な分布については不明な点も多い。 本研究では、「TnMERI1」型の水銀耐性トランスポゾンを指標として、トランスポゾンが世界各地の異なる細菌間で広く共有されていることを明らかにした。またトランスポゾンの定量法を検討し、土壌におけるトランスポゾンの不均一な分布を定量的に示した。これらの結果より、野外における遺伝子の水平伝播現象を具体的に示すことができた。
- 淡水域におけるコイヘルペスウィルスの生残に関わる環境因子の解析日本学術振興会科学研究費補助金若手研究（スタートアップ）研究期間 : 2006年09月 -2008年03月代表者 : 松井 一彰
- 日本学術振興会：科学研究費助成事業 若手研究(スタートアップ)研究期間 : 2006年 -2007年代表者 : 松井 一彰環境中におけるコイヘルペスウィルスの動態と環境因子の関係を明らかにするために、昨年に引き続き以下の順序で研究を進めた。 1.世界各地でのコイヘルペスウィルスの発生および検出に関する報告を経時的に整理し、コイヘルペスウィルス病の歴史とその発生に関連があると思われる環境因子の抽出を試みた。この作業を通じて環境因子とコイヘルペスウィルスの関連性については、ほとんど関連づけた考察がされていなかった事が明らかになった。 2.次に環境中よりコイヘルペスウィルスを回収する方法の検討を行った。環境中に存在するコイヘルペスウイルスを回収する方法については全く報告が無い事を受け、他の病原性ウィルスの環境水からの検出方法を整理し、その応用を検討した。そして上記1の項目と合わせてとりまとめたものをイギリスのFreshwater Biology誌に報告した(現在印刷中オンライン版:doi:10.1111/j.1365-2427.2007.01874.x)。 3.野外環境より約80個体の野生ゴイを採取し、2で検討したコイヘルペスウイルス検出方法を利用して、野生ゴイからのコイヘルペスウイルス検出と,抗体価を利用した野生コイのコイヘルペスウイルス感染履歴を照らし合わせて評価し、野外における大発生から2年以上経った現在でも、野生コイの約半数がコイヘルペスウィルスを保持している事を明らかにした(現在投稿準備中)。 これらの結果から、コイヘルペスウィルスは今も野生コイの体内に広く生残しており、今後コイヘルペスウィルスへの感染経験が無く、抗体を持たないコイが増えてくるにつれて、再びコイヘルペス症が大発生する可能性がある事が考えられた。
- 日本学術振興会：科学研究費助成事業 特別研究員奨励費研究期間 : 2003年 -2005年代表者 : 松井 一彰細菌が持つ環境浄化に有効な遺伝子を、最近が本質的にもつ遺伝形質伝播機構を利用して汚染環境中の土着細菌群集に伝播させる「新規環境浄化法」開発のための基礎的知見の提供を目的に研究をおこない、以下のような知見を得ることができた。 1.Bacillus属細菌がもつ有機水銀分解能をコードしている可動性遺伝子(トランスポゾン:TnMERI1)を対象に、TnMERI1の転移能力の検証をおこなった。その結果TnMERI1が実際に転移能力を有しており、伝達性プラスミドを介して細菌間で伝播されることを実験的に再現できた。またTnMERI1の転移は、紫外線照射やヒートショック等による細菌へのストレス付加に影響されにくい現象であることも分かった。これらの知見は論文としてまとめ、FEMS Microbiology Letters誌に発表した。 2.上記TnMERI1様トランスポゾンを持つBacillus属細菌を、世界各地の土壌より発見する事ができた。さらに詳細な菌株の同定やトランスポゾン近辺の塩基配列解析をおこなった結果、TnMERI1様トランスポゾンを持つ同一菌株が広く世界中に拡散されている事がわかった。また自然環境中の細菌間では、TnMERI1様トランスポゾンが水平伝播され、拡散していることもわかった。以上の結果から、TnMERI1様トランスポゾンの水平伝播は世界中で起きている普遍的な現象であり、TnMERI1を有機水銀汚染環境の土着細菌に伝播させて、細菌による有機水銀汚染の浄化を促進させる事は、安全かつ有効な浄化方法であると考えられる。これらの知見は現在論文としてとりまとめており、成果の一部は2006年度の国際学会(国際微生物生態学会)にて発表予定である。