FUKUDA Takashi

Department of FisheriesProfessor

Last Updated :2024/07/20

■Researcher comments

List of press-related appearances

1

■Researcher basic information

Degree

  • Ph.D(2006/03 Gifu University)

Research Field

  • Life sciences / Pharmaceuticals - chemistry and drug development

■Research activity information

Award

  • 2016/10 日本薬学会生薬天然物部会 A research award
     Yellow pigment inhibitors from microorganisms 
    受賞者: Fukuda Takashi

Paper

  • Ryota Okamura; Katsuki Kikuchi; Akito Taniguchi; Kenichiro Nagai; Reiko Seki; Satoshi Ohte; Taichi Ohshiro; Masashi Ando; Teruyoshi Tanaka; Takashi Fukuda
    The Journal of antibiotics 2024/05 
    Seriniquinone was isolated as a melanoma-selective anti-cancer agent from a culture broth of the marine-derived bacterium Serinicoccus marinus CNJ927 in 2014. It targets the unique small protein, dermcidin, which affects the drug resistance of cancer cells. Due to its significant activity against cancer cells, particularly melanoma, and its unique target, seriniquinone has been developed as a new pharmacophore. However, it has the disadvantage of poor solubility in drug discovery research, which needs to be resolved. A new seriniquinone glycoside (1) was synthesized by the biological transformation of seriniquinone using the deep sea-derived bacterium Bacillus licheniformis KDM612. Compound 1 exhibited selective anti-cancer activity against melanoma, similar to seriniquinone, and was 50-fold more soluble in DMSO than seriniquinone.
  • Kenichiro Nagai; Keisuke Kobayashi; Ryosuke Miyake; Yukino Sato; Reiko Seki; Takashi Fukuda; Akiho Yagi; Ryuji Uchida; Taichi Ohshiro; Hiroshi Tomoda
    The Journal of antibiotics 2024/01 [Refereed]
     
    Nectriatide 1a, a naturally occurring cyclic tetrapeptide, has been reported to a potentiator of amphotericin B (AmB) activity. In order to elucidate its structure-activity relationships, we synthesized nectriatide derivatives with different amino acids in solution-phase synthesis and evaluated AmB-potentiating activity against Candida albicans. Among them, C-and N-terminal protected linear peptides were found to show the most potent AmB-potentiating activity.
  • Moeka Uemura; Keisuke Kobayashi; Noriko Sato; Kenichiro Nagai; Reiko Seki; Michiya Kamio; Takashi Fukuda; Taishi Tsubouchi; Hiroshi Tomoda; Taichi Ohshiro; Takeshi Kobayashi; Takeshi Terahara
    The Journal of Antibiotics Springer Science and Business Media LLC 0021-8820 2023/09 [Refereed]
  • Masashi Ando; Wen Jye Mok; Yuji Maeda; Ryoji Miki; Takashi Fukuda; Yasuyuki Tsukamasa
    Food Science & Nutrition Wiley 10 (9) 3024 - 3033 2048-7177 2022/09 [Refereed]
  • Miyuki Konya; Shiho Arima; Daiki Lee; Masaki Ohtawa; Kenta Shimoyama; Takashi Fukuda; Ryuji Uchida; Hiroshi Tomoda; Noriyuki Yamaotsu; Nobutada Tanaka; Tohru Nagamitsu
    Chemical and Pharmaceutical Bulletin Pharmaceutical Society of Japan 70 (4) 261 - 268 0009-2363 2022/04 [Refereed]
     
    Habiterpenol is a G2 checkpoint inhibitor isolated from the culture broth of Phytohabitans sp. 3787_5. Here, we report the synthesis of new habiterpenol analogs through the total synthesis process of habiterpenol and evaluating the analogs for G2 checkpoint inhibitory activity. We investigated two different synthetic approaches for total synthesis, with intramolecular conjugate addition and Ti(III)-mediated radical cyclization as key reactions. Although the former was unsuccessful, the latter reaction facilitated stereoselective total synthesis and determination of the absolute configuration of habiterpenol. The extension of these chemistries to a structure-activity relationship (SAR) study gave new habiterpenol analogs, which could not be derived from natural habiterpenol and only be synthesized by applying the total synthesis. Therefore, this study provides important insights into SAR studies of habiterpenol.
  • Kohei Ishida; Teruki Tanaka; Kenichiro Nagai; Yoshimasa Furuichi; Takeshi Terahara; Masashi Ando; Yasuyuki Tsukamasa; Takashi Fukuda
    The Journal of Antibiotics Springer Science and Business Media LLC 75 (1) 9 - 15 0021-8820 2022/01 [Refereed]
  • SHUHEI NISHIGUCHI; TAKASHI FUKUDA; MASASHI ANDO; YASUYUKI TSUKAMASA
    NIPPON SUISAN GAKKAISHI Japanese Society of Fisheries Science 86 (6) 494 - 501 0021-5392 2020/11 [Refereed]
  • Takashi Fukuda; Kenichiro Nagai; Akihiko Kanamoto; Hiroshi Tomoda
    The Journal of Antibiotics Springer Science and Business Media LLC 73 (8) 548 - 553 0021-8820 2020/08 [Refereed]
  • Miyuki Konya; Kenta Shimoyama; Shiho Arima; Takashi Fukuda; Ryuji Uchida; Hiroshi Tomoda; Tohru Nagamitsu
    Organic Letters American Chemical Society (ACS) 22 (13) 5131 - 5134 1523-7060 2020/07 [Refereed]
  • Masashi Ando; Takahiro Yamada; Yoichiro Okinaga; Etsuko Taguchi; You Sugimoto; Akiko Takeuchi; Tomohiro Itoh; Takashi Fukuda; Yasuyuki Tsukamasa
    Food Chemistry Elsevier BV 303 125351 - 125351 0308-8146 2020/01 [Refereed]
  • Fukuda T; Nagai K; Yagi A; Kobayashi K; Uchida R; Yasuhara T; Tomoda H
    Journal of Natural Products 82 (10) 2673 - 2681 2019/09 [Refereed]
     
    A new compound, designated nectriatide (1), was isolated as a potentiator of amphotericin B (AmB) activity against Candida albicans from the culture broth of Nectriaceae sp. BF-0114. This structure was elucidated based on spectroscopic analyses (1D and 2D NMR data), chemical methods, and total synthesis. Compound 1 was a unique cyclotetrapeptide consisting of l-N-methyltyrosine, anthranilic acid, l-alanine, and l-valine. Compound 1 and several synthetic derivatives, including linear peptides, potentiated AmB activity against C. albicans by up to 16-fold (the MIC value of AmB decreased from 0.5 μg/mL to 0.031 μg/mL in combination with test compound).
  • Ohshiro T; Seki R; Fukuda T; Uchida R; Tomoda H
    The Journal of antibiotics 71 (12) 1000 - 1007 0021-8820 2018/11 [Refereed]
     
    New indanones, designated celludinones A ((±)-1) and B (2), were isolated from the culture broth of the fungal strain Talaromyces cellulolyticus BF-0307. The structures of celludinones were elucidated by spectroscopic data, including 1D and 2D NMR. Celludinone A was found to be a mixture of racemic isomers ((±)-1), which were isolated by a chiral column. Compounds (+)-1 and (-)-1 inhibited the sterol O-acyltransferase (SOAT) 1 and 2 isozymes in a cell-based assay using SOAT1- and SOAT2-expressing Chinese hamster ovary (CHO) cells, while 2 selectively inhibited the SOAT2 isozyme.
  • Fukuda T; Furukawa T; Kobayashi K; Nagai K; Uchida R; Tomoda H
    The Journal of antibiotics 72 (1) 8 - 14 0021-8820 2018/10 [Refereed]
  • Yasuyuki Tsukamasa; Kohei Nakamura; Tatsuro Nagato; Keigoro Yamamoto; Tomokazu Morita; Tomoki Hiraoka; Takashi Fukuda; Tomohiro Itoh; Masashi Ando
    Nippon Suisan Gakkaishi (Japanese Edition) Nihon Suisan Gakkai 84 (1) 111 - 118 1349-998X 2018 [Refereed]
     
    The eŠect of temperature treatment before thawing on the delay of discoloration of frozen skipjack Katsuwonus pelamis meat after thawing was investigated. The NAD hydrolyzing activities of skipjack meat at -6°C and -8°C were 3.66×10-3 mmol/(g min) and 2.78×10-3 mmol/(g min), respectively. After 24 h temperature treatment at -6°C and -8°C, NAD+ concentrations of the meat decreased by up to 3.8%and 5.2%, respectively. After 1-day storage at 5°C, pH of the untreated meat reached around 5.6 however, that of the samples treated at -6°C and -8°C was around 6.3. After 1-day storage at 5°C, metmyoglobin concentrations of meats treated at either temperature with vacuum packaging did not increase however, those of untreated meat and meats treated at the same temperatures with air packaging increased significantly.
  • 塚正泰之; 山下洋; 高島秋則; 松浦良平; 安藤正史; 福田隆志; 山本眞司; 那須敏郎; 有路昌彦; 升間主計
    水産増殖 66 (3) 235 - 242 0371-4217 2018 [Refereed]
  • Chlokamycin, a new chloride from the Marine-derived Streptomyces sp. MA2-12.
    Fukuda T; Takahashi M; Kasai H; Nagai K; Tomoda H
    Nat. Prod. Com. 12 1 - 4 2017/07 [Refereed]
  • Takashi Fukuda; Misaki Takahashi; Kenichiro Nagai; Enjuro Harunari; Chiaki Imada; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 70 (5) 590 - 594 0021-8820 2017/05 [Refereed]
     
    A new cytotoxic agent designated isomethoxyneihumicin (1 and 2), a mixture of lactam-lactim tautomers, was isolated along with methoxyneihumicin (3) from the culture broth of the marine Nocardiopsis alba KM6-1. The structures of 1 and 2 were elucidated in spectroscopic analyses (1D and 2D NMR data, and ROESY correlations). Isomethoxyneihumicin (15.0 mu M) and 3 (15.0 mu M) arrested the cell cycle of Jurkat cells at the G2/M phase (66 and 67%) in 12 h. Isomethoxyneihumicin and 3 exhibited cytotoxicity against Jurkat cells with IC50 values of 6.98 and 30.5 mu M in 20 h, respectively. These results strongly suggest that isomethoxyneihumicin and 3 exhibit cytotoxicity against Jurkat cells by inhibiting the cell cycle at the G2/M phase.
  • Aika Suzuki; Takashi Fukuda; Keisuke Kobayashi; Taichi Ohshiro; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 70 (1) 96 - 97 0021-8820 2017/01 [Refereed]
  • Yaojun Tong; Mei Liu; Yu Zhang; Xueting Liu; Ren Huang; Fuhang Song; Huanqin Dai; Biao Ren; Nuo Sun; Gang Pei; Jiang Bian; Xin-Ming Jia; Guanghua Huang; Xuyu Zhou; Shaojie Li; Buchang Zhang; Takashi Fukuda; Hiroshi Tomoda; Satoshi Ōmura; Richard D. Cannon; Richard Calderone; Lixin Zhang
    Synthetic and Systems Biotechnology Elsevier BV 1 (3) 158 - 168 2405-805X 2016/09 [Refereed]
  • Takun Furukawa; Takashi Fukuda; Kenichiro Nagai; Ryuji Uchida; Hiroshi Tomoda
    NATURAL PRODUCT COMMUNICATIONS NATURAL PRODUCTS INC 11 (7) 1001 - 1003 1934-578X 2016/07 [Refereed]
     
    The fungus, Aspergillzts nidulans BF0142, was isolated from hot spring-derived soil collected at Hell Valley in Noboribetsu, Hokkaido, Japan. A new furanone compound designated helvafuranone (1) was isolated along with microperfuranone (2), 9-hydroxymicroperfuranone (3), diorcinol (4), emestrin (5), and sterigmatocystin (6) from a culture broth of A. nidulans BF0142. The structure of 1 was elucidated as 5-hydroxy-4-(4-hydroxybenzyl)-3-(4hydroxybenzyl)furanone based on various NMR experiments and chemical modifications.
  • Takashi Fukuda; Minori Shinkai; Eri Sasaki; Kenichiro Nagai; Yuko Kurihara; Akihiko Kanamoto; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 68 (10) 620 - 627 0021-8820 2015/10 [Refereed]
     
    Eight new thiodiketopiperazines, designated as graphiumins A to H (1-8), were isolated along with bisdethiobis(methylthio)-deacetylaranotin (9) and bisdethiobis(methylthio)-deacetylapoaranotin (10) from the culture broth of the marine-derived fungus Graphium sp. OPMF00224. The structures of the graphiumins were elucidated based on spectroscopic analyses (1D and 2D NMR data, ROESY correlations and CD data) and chemical methods. The absolute configuration of the common (3S)-3-hydroxyoctanoyl acid residue in 1, 3 and 4 was determined by hydrolysis, benzoyl derivatization and HPLC analysis using a chiral column. Five graphiumins moderately inhibited yellow pigment production by methicillin-resistant Staphylococcus aureus.
  • Keisuke Kobayashi; Takashi Fukuda; Takeshi Terahara; Enjuro Harunari; Chiaki Imada; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 68 (10) 638 - 641 0021-8820 2015/10 [Refereed]
  • Keisuke Kobayashi; Takashi Fukuda; Takeo Usui; Yuko Kurihara; Akihiko Kanamoto; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 68 (2) 126 - 132 0021-8820 2015/02 [Refereed]
     
    Marine-derived Streptomyces sp. OPMA00072 was found to produce inhibitors of the synthesis of neutral lipids in a cell-based assay using Chinese hamster ovary (CHO) cells. A new 16-membered macrolide named bafilomycin L (BFL) (1) was isolated along with the known structurally related bafilomycin C-1 (BFC1) (3) from the culture broth of the actinomycete by solvent extraction, octadecylsilyl column chromatography and HPLC. BFL inhibited cholesteryl ester (CE) synthesis in CHO cells with an IC50 value of 0.83 nm and also in mouse peritoneal macrophages with an IC50 of 6.1 nm. In addition, BFL blocked cellular acidification in He La cells by interfering with vacuolar H+-ATPase (V-ATPase) as well as other bafilomycins. These data strongly suggest that BFL disturbed the lysosome function to block cholesterol metabolism, leading to the inhibition of CE accumulation in mammalian cells.
  • Takashi Fukuda; Kenichiro Nagai; Yuko Kurihara; Akihiko Kanamoto; Hiroshi Tomoda
    Natural Product Sciences Korean Society of Pharmacognosy 21 (4) 255 - 260 1226-3907 2015 [Refereed]
     
    Two new thiodiketopiperazines (TDKPs), designated graphiumins I (1) and J (2), were isolated from the culture broth of the marine-derived fungus Graphium sp. OPMF00224 by solvent extraction, silica gel column chromatography, and HPLC. Their absolute structures were elucidated by spectroscopic analyses (1D and 2D NMR data, ROESY correlations, and CD data) and chemical methods. They were found to be structurally rare TDKPs with a phenylalanine-derived indolin substructure. Compounds 1 and 2 inhibited yellow pigment production by methicillin-resistant Staphylococcus aureus (MRSA) with IC50 values of 63.5 and 76.5 µg/ml, respectively, without inhibiting its growth, even at 250 µg/ml.
  • Lynnie Trzoss; Takashi Fukuda; Letcia V. Costa-Lotufo; Paula Jimenez; James J. La Clair; William Fenical
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA NATL ACAD SCIENCES 111 (41) 14687 - 14692 0027-8424 2014/10 [Refereed]
     
    Natural products continue to provide vital treatment options for cancer. Although their translation into chemotherapeutics is complex, collaborative programs continue to deliver productive pipelines for cancer chemotherapy. A new natural product, seriniquinone, isolated from a marine bacterium of the genus Serinicoccus, demonstrated potent activity over a select set of tumor cell lines with particular selectivity toward melanoma cell lines. Upon entering the cell, its journey began by localization into the endoplasmic reticulum. Within 3 h, cells treated with seriniquinone underwent cell death marked by activation of autophagocytosis and gradually terminated through a caspase-9 apoptotic pathway. Using an immunoaffinity approach followed by multipoint validation, we identified the target of seriniquinone as the small protein, dermcidin. Combined, these findings revealed a small molecule motif in parallel with its therapeutic target, whose potential in cancer therapy may be significant. This discovery defines a new pharmacophore that displayed selective activity toward a distinct set of cell lines, predominantly melanoma, within the NCI 60 panel. This selectivity, along with the ease in medicinal chemical modification, provides a key opportunity to design and evaluate new treatments for those cancers that rely on dermcidin activity. Further, the use of dermcidin as a patient preselection biomarker may accelerate the development of more effective personalized treatments.
  • Takashi Fukuda; Yuko Kurihara; Akihiko Kanamoto; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 67 (8) 593 - 595 0021-8820 2014/08 [Refereed]
  • Takashi Fukuda; Kenta Shimoyama; Tohru Nagamitsu; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 67 (6) 445 - 450 0021-8820 2014/06 [Refereed]
     
    Citridone A (1), originally isolated as a potentiator of antifungal miconazole activity from a fungal culture broth, has a phenyl-R-furopyridone structure. Because of its unique ring structure, 11 derivatives were chemically synthesized and their biological activity was evaluated. Derivatives 17, 20 and 21 potentiated miconazole activity against Candida albicans. Furthermore, 1, 14, 20 and 21 were found to inhibit yellow pigment production in methicillin-resistant Staphylococcus aureus.
  • Junji Inokoshi; Naoki Shigeta; Takashi Fukuda; Ryuji Uchida; Kenichi Nonaka; Rokurou Masuma; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 66 (9) 549 - 554 0021-8820 2013/09 [Refereed]
     
    A new compound, designated epi-trichosetin (1), was isolated along with the known compound trichosetin (2) from the culture broth of Fusarium oxysporum FKI-4553 by solvent extraction, silica gel column chromatography and reversed-phase HPLC. The structure of 1 was elucidated by comparing various spectral data with those of 2, revealing that 1 was a stereoisomer of 2. Compounds 1 and 2 inhibited the undecaprenyl pyrophosphate synthase activity of Staphylococcus aureus with IC50 values of 83 and 30 mu M, respectively, and showed antimicrobial activity, particularly against Gram-positive bacteria, including methicillin-sensitive and -resistant S. aureus.
  • Takashi Fukuda; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 66 (6) 355 - 357 0021-8820 2013/06 [Refereed]
  • Amy L. Lane; Sang-Jip Nam; Takashi Fukuda; Kazuya Yamanaka; Christopher A. Kauffman; Paul R. Jensen; William Fenical; Bradley S. Moore
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY AMER CHEMICAL SOC 135 (11) 4171 - 4174 0002-7863 2013/03 [Refereed]
     
    Cyanosporasides are marine bacterial natural products containing a chlorinated cyclopenta[a]indene core of suspected enediyne polyketide biosynthetic origin. Herein, we report the isolation and characterization of novel cyanosporasides C-F (3-6) from the marine actinomycetes Salinispora pacifica CNS-143 and Streptomyces sp. CNT-179, highlighted by the unprecedented C-2' N-acetylcysteamine functionalized hexose group of 6. Cloning, sequencing, and mutagenesis of homologous similar to 50 kb cyanosporaside biosynthetic gene clusters from both bacteria afforded the first genetic evidence supporting cyanosporaside's enediyne, and thereby p-benzyne biradical, biosynthetic origin and revealed the molecular basis for nitrile and glycosyl functionalization. This study provides new opportunities for bioengineering of enediyne derivatives and expands the structural diversity afforded by enediyne gene clusters.
  • Mio Kawaguchi; Ryuji Uchida; Satoshi Ohte; Natsuki Miyachi; Keisuke Kobayashi; Noriko Sato; Kenichi Nonaka; Rokuro Masuma; Takashi Fukuda; Tadashi Yasuhara; Hiroshi Tomoda
    The Journal of antibiotics 66 (3) 179 - 89 0021-8820 2013/03 [Refereed]
     
    Eight new dinapinones, AB1, AB2, AC1, AC2, AD1, AD2, AE1 and AE2, were isolated from the culture broth of Talaromyces pinophilus FKI-3864. The structures of these dinapinones were elucidated by various NMR experiments. All these dinapinones possessed the same biaryl dihydronaphthopyranone skeleton consisting of a heterodimer with one monapinone A and one different monapinone. Dinapinones AB1 and AB2, consisting of monapinones A and B, were atropisomers. Similarly, dinapinones AC1 and AC2, consisting of monapinones A and C, dinapinones AD1 and AD2, consisting of monapinones A and D, and dinapinones AE1 and AE2, consisting of monapinones A and E, were atropisomers. Dinapinone AB2 showed potent inhibition of triacylglycerol (TG) synthesis in intact mammalian cells with an IC50 value of 1.17 μM, whereas the other dinapinones showed weak inhibition of TG synthesis.
  • Mio Kawaguchi; Takashi Fukuda; Ryuji Uchida; Kenichi Nonaka; Rokuro Masuma; Hiroshi Tomoda
    Journal of Antibiotics 66 (1) 23 - 29 0021-8820 2013/01 [Refereed]
     
    Cylindrol A 5, a new ascochlorin congener, was isolated along with 14 known compounds from the culture broth of Cylindrocarpon sp. FKI-4602 by solvent extraction, octadecylsilane column chromatography and HPLC. The structure of cylindrol A 5 was elucidated by spectral analyses, including NMR. The compound has an ascochlorin skeleton consisting of a resorcin aldehyde and a cyclohexanone moieties. Cylindrol A 5 showed moderate antimicrobial activity against Bacillus subtilis, Kocuria rhizophila, Mycobacterium smegmatis and Acholeplasma laidlawii. The biosynthetic pathway to cylindrol A 5 was deduced from the 14 isolated metabolites of the fungal strain. © 2013 Japan Antibiotics Research Association All rights reserved.
  • Takashi Fukuda; Kenichiro Nagai; Hiroshi Tomoda
    JOURNAL OF NATURAL PRODUCTS AMER CHEMICAL SOC 75 (12) 2228 - 2231 0163-3864 2012/12 [Refereed]
     
    Racemates of the diphenolic metabolites (+/-)-tylopilusin A (1) and (+/-)-tylopilusin B (2) were isolated from the fruiting bodies of Tylopilus eximius. Their structures were elucidated on the basis of spectroscopic analyses (1D and 2D NMR data and ROESY correlations) and X-ray crystallography. Each racemate was separated into its individual enantiomers, and electronic circular dichroism calculations were used to assign the absolute configuration of (+)- and (-)-tylopilusin A (1) and (+)- and (-)-tylopilusin B (2).
  • Takashi Fukuda; Ryuji Uchida; Satoshi Ohte; Hiroyo Inoue; Hiroyuki Yamazaki; Daisuke Matsuda; Kenichi Nonaka; Rokurou Masuma; Takenobu Katagiri; Hiroshi Tomoda
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 65 (11) 565 - 569 0021-8820 2012/11 [Refereed]
     
    Two new butenolides, designated trichocyalides A and B, were isolated along with the known compound harzianolide, from the culture broth of Trichoderma sp. FKI-5513 by solvent extraction, ODS column chromatography and HPLC. Their structures were elucidated by several spectral analyses, showing that they have the common skeleton of butenofuranone. Trichocyalides A and B inhibited alkaline phosphatase (ALP) activity, a typical marker enzyme of osteoblastic differentiation (IC50: 83.0 and 187 mu M, respectively), in bone morphogenetic protein (BMP)-stimulated C2C12 myoblasts mutant cells, which stably express BMP receptor activity, whereas harzianolide showed no inhibitory activity against ALP even at 500 mu M. The Journal of Antibiotics (2012) 65, 565-569; doi: 10.1038/ja.2012.70; published online 5 September 2012
  • Naoko Kubota; Yasutaka Inayoshi; Naoko Satoh; Takashi Fukuda; Kenta Iwai; Hiroshi Tomoda; Michinori Kohara; Kazuhiro Kataoka; Akira Shimamoto; Yasuhiro Furuichi; Akio Nomoto; Akira Naganuma; Shusuke Kuge
    FEBS LETTERS ELSEVIER SCIENCE BV 586 (16) 2318 - 2325 0014-5793 2012/07 [Refereed]
     
    Hepatitis C virus core protein (Core) contributes to HCV pathogenicity. Here, we demonstrate that Core impairs growth in budding yeast. We identify HSP90 inhibitors as compounds that reduce intracellular Core protein level and restore yeast growth. Our results suggest that HSC90 (Hsc82) may function in the protection of the nascent Core polypeptide against degradation in yeast and the C-terminal region of Core corresponding to the organelle-interaction domain was responsible for Hsc82-dependent stability. The yeast system may be utilized to select compounds that can direct the C-terminal region to reduce the stability of Core protein. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
  • Fukuda T; Uchida R; Inoue H; Ohte S; Yamazaki H; Matsuda D; Katagiri T; Tomoda H
    Acta Pharmaceutica Sinica B Elsevier {BV} 2 (1) 23 - 27 2211-3835 2012/02 [Refereed]
  • Mayumi Kaneko; Daisuke Matsuda; Masaki Ohtawa; Takashi Fukuda; Tohru Nagamitsu; Takao Yamori; Hiroshi Tomoda
    BIOLOGICAL & PHARMACEUTICAL BULLETIN PHARMACEUTICAL SOC JAPAN 35 (1) 18 - 28 0918-6158 2012/01 [Refereed]
     
    Most cancer cells have mutations in genes at the Cl checkpoint and repair DNA only in the 62 phase; therefore, the 62 checkpoint is a potential target to develop novel therapy. In the course of screening, a known compound, pycnidione, was isolated from the fungal culture broth of Gloeotinia sp. FKI-3416. Pycnidione irreversibly abrogated bleomycin-induced G2 arrest in Jurkat cells and synergically potentiated the cytotoxicity of bleomycin. To elucidate the mechanism of action, the effect of pycnidione on the signal transduction of the 62 checkpoint was analyzed, showing that the increased phospho-cyclin dependent kinase-1 (CDK1) level caused by bleomycin was abrogated in the presence of pycnidione, indicating that cells did not arrest at the 62 phase. Moreover, under these conditions, Chk1 and Chk2 levels were markedly down-regulated. Thus, we concluded that pycnidione abrogated bleomycin-induced 62 arrest by decreasing Chk1 and Chk2.
  • Kent Sakai; Nobuhiro Koyama; Takashi Fukuda; Yukiko Mori; Hiroyasu Onaka; Hiroshi Tomoda
    BIOLOGICAL & PHARMACEUTICAL BULLETIN PHARMACEUTICAL SOC JAPAN 35 (1) 48 - 53 0918-6158 2012/01 [Refereed]
     
    Staphyloxanthin, a yellow pigment produced by methicillin-resistant Staphylococcus aureus (M RSA), is a virulent factor escaping from the host immune system. A new screening method for inhibitors of staphyloxanthin production by MRSA was established using paper disks. By this screening method, inhibitors of staphyloxanthin production were selected from the natural product library (ca. 300) and from actinomycete culture broths (ca. 1000). From the natural product library, four known inhibitors of lipid metabolism, cerulenin, dihydrobisvertinol, xanthohumol and zaragozic acid, were found to inhibit staphyloxanthin production; however, typical antibiotics used clinically, including vancomycin, had no effect on staphyloxanthin production. From actinomycete culture broths, two known anthraquinones, 6-deoxy-8-O-methylrabelomycin and tetrangomycin, were found to inhibit staphyloxanthin production by MRSA in the paper disk assay. These results suggested that this screening method is useful and effective to find compounds targeting staphyloxanthin production, leading to a new type of chemotherapeutics against MRSA infection.
  • Takashi Fukuda; Eric D. Miller; Benjamin R. Clark; Ali Alnauman; Cormac D. Murphy; Paul R. Jensen; William Fenical
    JOURNAL OF NATURAL PRODUCTS AMER CHEMICAL SOC 74 (8) 1773 - 1778 0163-3864 2011/08 [Refereed]
     
    Three polyenylpyrone metabolites, pyridinopyrones A to C (1-3), have been isolated from the culture broth of a marine-derived Streptomyces sp., strain CNQ-301. The structures of the pyridinopyrones were assigned on the basis of chemical modification and combined spectroscopic methods, focusing on interpretation of 1D and 2D NMR data. Pyridinopyrones B and C (2, 3), examined as an inseparable mixture of methyl positional isomers, were ultimately defined by hydrogenation and NMR analysis of a saturated derivative. The biosynthesis of these metabolites was defined by the incorporation of stable isotope-labeled precursors, revealing that the biosynthetic starter unit is nicotinic acid, while the polyene chain and pendant methyl groups are acetate- and methionine-derived, respectively.
  • Tomoko Miyagawa; Keisuke Nagai; Asami Yamada; Yoshinori Sugihara; Takeo Fukuda; Takashi Fukuda; Ryuji Uchida; Hiroshi Tomoda; Satoshi Omura; Tohru Nagamitsu
    ORGANIC LETTERS AMER CHEMICAL SOC 13 (5) 1158 - 1161 1523-7060 2011/03 [Refereed]
     
    The first total synthesis of citridone A has been achieved through regioselective intramolecular iodocyclization and regio- and stereoselective Pd(0)-catalyzed coupling as key reactions.
  • Takashi Fukuda; Yoko Hasegawa; Yasunari Sakabe; Hiroshi Tomoda; Satoshi Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 61 (9) 550 - 555 0021-8820 2008/09 [Refereed]
     
    Two new aromatic alkaloids, designated citrinamides A and B, were isolated from the culture broth of Penicillium sp. FKI-1938 by solvent extraction, silica gel column chromatography and HPLC. Their structures were elucidated by spectroscopic analysis, including NMR and amino acid analysis. Citrinamides A and B showed moderate potentiation of miconazole activity against Candida albicans.
  • Takanori Matsumaru; Toshiaki Sunazuka; Tomoyasu Hirose; Aki Ishiyama; Miyuki Namatame; Takashi Fukuda; Hiroshi Tomoda; Kazuhiko Otoguro; Satoshi Omura
    TETRAHEDRON PERGAMON-ELSEVIER SCIENCE LTD 64 (30-31) 7369 - 7377 0040-4020 2008/07 [Refereed]
     
    The first, concise total synthesis of (+/-)-tensyuic acids B, C, and E, using chemoselective formal S(N)2' type Grignard reactions and selective esterification, is described. In addition, the optical purity of natural (+/-)tensyuic acid B was determined using Chirabite-AR. Synthetic tensyuic acids, together with their intermediate compounds, were found to possess useful bioactive properties, with some of them showing potent activity against Trypanosorna brucei brucei strain GUTat 3.1. (c) 2008 Elsevier Ltd. All rights reserved.
  • Yasuhiro Kojima; Toshiaki Sunazuka; Kenichiro Nagai; Khachatur Julfakyan; Takashi Fukuda; Hiroshi Tomoda; Satoshi Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 61 (5) 297 - 302 0021-8820 2008/05 [Refereed]
     
    Total synthesis of a fungal cyclic peptide, malformin C, recently rediscovered as a G2 checkpoint inhibitor was completed. Our synthesis involved a convergent approach with respect to a linear pentapeptide, cyclization, and oxidative disulfide formation.
  • Yoko Hasegawa; Takashi Fukuda; Keiichi Hagimori; Hiroshi Tomoda; Satoshi Omura
    CHEMICAL & PHARMACEUTICAL BULLETIN PHARMACEUTICAL SOC JAPAN 55 (9) 1338 - 1341 0009-2363 2007/09 [Refereed]
     
    Six new alkylitaconic acids, designated tensyuic acids A to F, were isolated from the culture broth of Aspergillus niger FKI-2342 by solvent extraction, silica gel column chromatography and HPLC. Their structures were elucidated by spectroscopic analysis including UV, NMR, and MS. They are all alkylitaconic acid derivatives. Only tesyuic acid C showed moderate antimicrobial activity against Bacillus subtilis.
  • Keiichi Hagimori; Takashi Fukuda; Yoko Hasegawa; Satoshi Omura; Hiroshi Tomoda
    BIOLOGICAL & PHARMACEUTICAL BULLETIN PHARMACEUTICAL SOC JAPAN 30 (8) 1379 - 1383 0918-6158 2007/08 [Refereed]
     
    A DNA-damaging agent, bleomycin, arrests the cell cycle at the G2 phase of Jurkat cells, which are defective in the G1 checkpoint, while microtubule-disrupting colchicine arrests it at M phase. Fungal cyclopeptides, malformin A 1 and malformin C, were found to abrogate bleomycin-induced G2 arrest (IC50; 0.48 mu M and 0.9 nM, respectively), resulting in a drastic decrease in cells in G2 phase and increase in cells in subG1 phase. On the other hand, malformins showed little effect on the colchicine-induced M phase arrest in Jurkat cells (IC50; 2.7 mu M and 24 nM, respectively). Malformin C (0.026 mu M) also abrogated bleomycin-induced G2 arrest in colon cancer-derived HCT-116 cells. These data strongly suggest that malformin C disrupted the cell cycle at the G2 checkpoint of cancer cells, leading to sensitization of the cancer cells to the anti-cancer reagent.
  • Takashi Fukuda; Yasunari Sakabe; Hiroshi Tomoda; Satoshi Omura
    CHEMICAL & PHARMACEUTICAL BULLETIN PHARMACEUTICAL SOC JAPAN 54 (12) 1659 - 1661 0009-2363 2006/12 [Refereed]
     
    Citridone D was isolated from the culture broth of Penicillium sp. FKI-1938 by solvent extraction, silica gel column chromatography and HPLC. The structure of citridone D was elucidated by spectroscopic analysis including NMR analysis. Citridone D was found to have a novel phenylfuropyridine skeleton different from those of other citridones. Citridone D potentiated miconazole activity against Candida albicans.
  • Fukuda Takashi; Masuma Rokuro; Tomoda Hiroshi; Nagamitsu Tohru; Omura Satoshi
    Symposium on the Chemistry of Natural Products, symposium papers Symposium on the chemistry of natural products (48) 277 - 282 2006/09 
    On the basis of a new concept of anti-infective drugs, assay systems were conducted to screen for compounds which potentiate the antifungal activity of an azole compound (miconazole) or the anti-MRSA activity of a β-lactam (imipenem), as a potential approach for the development of anti-infective drugs. Using the conventional assay system, several new compounds, such as beauvericins and phenatic acids were discovered. During continuous screening work, a culture broth of Penicillium sp. FKI-1938, isolated from soil collected at Ishigakijima, was recently selected. Five structurally-related new compounds named citridones were isolated from the culture broth (5L) through EtOAc extract, silicagel column chromatography and preparative HPLC. Finally, citridone A (4.7mg), a mixture of citridones B and B' (59.2mg, purified as a mixture of hemiacetal epimers) and citridone C (11.7mg) were obtained as white needles or amorphous. From another jar fermentation broth (20L), citridone D (16.4mg) was obtained as amorphous. The molecular formulas of citridones B and B' were determined to be C_<19>H_<21>NO_5 on the basis of HRFAB-MS measurement. The structures were elucidated by spectroscopic analyses including various NMR experiments and X-ray crystallography of a tri-O-acetyl derivative prepared from citridone B. Citridones B and B' have a novel cyclic ring system of 7-phenylfuropyridin-2-ol fused with a furan ring. All citridones showed growth inhibition of C. albicans KF-1, but only in combination with miconazole (0.06μM) by the paper disk method. Furthermore, only citridone A potentiated β-lactam imipenem activity against MRSA by decreasing the MIC value from 100μM to 1.6μM.
  • Takashi Fukuda; Yoko Hasegawa; Keiichi Hagimori; Yuichi Yamaguchi; Rokuro Masuma; Hiroshi Tomoda; Satoshi Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 59 (8) 480 - 485 0021-8820 2006/08 [Refereed]
     
    Two new furopyrrols, designated tensidols A and B, were isolated from the culture broth of Aspergillus niger FKI-2342 by solvent extraction, silica gel column chromatography and HPLC. Their structures were elucidated and shown to have the common skeleton of 6-benzyl-6H-furo[2,3-b]pyrrole. Tensidols A and B potentiated miconazole activity against Candida albicans. Tensidols also showed moderate antimicrobial activity only against Pyricularia oryzae.
  • Fukuda Takashi; Tomoda Hiroshi; Omura Satoshi
    International Symposium on the Chemistry of Natural Products Symposium on the chemistry of natural products 2006 "P - 527" 2006/07
  • T Fukuda; Y Yamaguchi; R Masuma; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 58 (5) 309 - 314 0021-8820 2005/05 [Refereed]
     
    New phenylfuropyridinones and related compounds, designated citridones A, B, B' and C, were isolated along with known CJ-16,173, from the culture broth of Penicillium sp. FKI-1938 by solvent extraction, silica gel column chromatography and HPLC. Citridones (75 μ M) potentiate the miconazole activity against Candida albicans, decreasing the IC50 value of miconazole from 14.5 nM to 3.5&SIM; 6.3 nM.
  • T Fukuda; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 58 (5) 315 - 321 0021-8820 2005/05 [Refereed]
     
    The structures of citridones A, B, B' and C, new potentiators of miconazole activity against Candida albicans produced by Penicillium sp. FKI-1938, were elucidated by various spectroscopic analyses including UV, NMR, and MS and degradation experiments. Although citridones B and B' were isolated as a mixture, each structure was also elucidated, indicating that they exist in equilibrium of hemiacetal epimerization. Citridones A, B and B' have a similar phenylfuropyridone moiety.
  • T Fukuda; A Matsumoto; Y Takahashi; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 58 (4) 252 - 259 0021-8820 2005/04 [Refereed]
     
    Two new phenols, designated phenatic acids A and B, were isolated along with known actiphenol, from the culture of Streptomyces sp. K03-0132 by solvent extraction, silica gel column chromatography and HPLC. Their structures were elucidated by spectroscopic analyses including mainly various NMR experiments. They have a common 1-hydroxy 2, 4-dimethyl benzene ring. These compounds potentiate miconazole activity against Candida albicans. Phenatic acid B also showed moderate antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Bacteroides fragilis and Acholeplasma laidlawii.
  • T Fukuda; M Arai; Y Yamaguchi; R Masuma; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOT RES ASSN 57 (2) 110 - 116 0021-8820 2004/02 [Refereed]
     
    Three new beauvericins, designated beauvericins D, E and F, were isolated along with known beauvericin and beauvericin A, from the culture of Beauveria sp. FKI-1366 by solvent extraction, ODS column chromatography and HPLC. These compounds potentiate miconazole activity against not only wild Candida albicans but also fluconazole resistant C. albicans. Beauvericins D and E decreased the IC50 value of miconazole against fluconazole resistant C. albicans from 1.3 mum to 0.25 and 0.31 mum, respectively.
  • T Fukuda; M Arai; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOT RES ASSN 57 (2) 117 - 124 0021-8820 2004/02 [Refereed]
     
    The structures of beauvericins D, E and F, novel potentiators of miconazole activity against Candida albicans produced by Beauveria sp. FKI 1366, were elucidated by various spectroscopic analyses including UV NMR, and MS and degradation experiments. They have the common skeleton of the 18-membered cyclodepsipeptides.
  • YP Kim; H Tomoda; K Iizima; T Fukuda; A Matsumoto; Y Takahashi; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOTICS RESEARCH ASSOC 56 (5) 448 - 453 0021-8820 2003/05 [Refereed]
     
    Three new pentaene macrolides having a 28-membered ring, designated takanawaenes A, B and C, were isolated from the fermentation broth of Streptomyces sp. K99-5278 by solvent extraction, silica-gel column chromatography and HPLC. Takanawaenes showed antifungal activity against Aspergillus niger, Mucor racemosus, Candida albicans and Saccharomyces cerevisiae.
  • T Fukuda; YP Kim; K Iizima; H Tomoda; S Omura
    JOURNAL OF ANTIBIOTICS JAPAN ANTIBIOT RES ASSN 56 (5) 454 - 458 0021-8820 2003/05 [Refereed]
     
    The structures of takanawacnes A, B and C, novel antifungal antibiotics produced by Streptomyces sp. K99-5278, were elucidated by various spectroscopic analyses including UV and NMR, and spectrometric analyses including MS. They have the common skeleton of a 28-membered pentaene macrolide.
  • H Shibata; T Fukuda; T Wada; Y Morita; T Hashimoto; Y Asakawa
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY TAYLOR & FRANCIS LTD 62 (7) 1432 - 1434 0916-8451 1998/07 [Refereed]
     
    A novel phenolic metabolite, ornatipolide, was isolated from the Boletus ornatipes fungus. Its structure was established by a combination of spectroscopic and chemical methods, and by an X-ray crystallographic analysis.

Books and other publications

  • The Society for Antibacterial and Antifungal Agents, Japan
    Takashi Fukuda (Single work天然物とその利用ー抗菌および抗真菌活性に関してー3抗真菌薬を賦活化する天然物)日本防菌防黴学会 2024/01
  • 抗真菌薬活性増強剤の開拓
    小林啓介; 内田龍児; 福田隆志; 長光亨; 長井賢一郎; 供田洋 (Joint work)化学工業社 2021/01
  • 供田, 洋; 黒田, 照夫; 大村, 智 (Contributor)南江堂 2018/02 9784524403493 xv, 310p
  • 天然物研究への蛍光指紋分析利用法の提案
    福田隆志 株式会社日立ハイテクノロジーズ 2017/09
  • 黄色ブドウ球菌が生産する黄色色素生成をターゲットとした阻害剤の探索
    福田隆志、小山信裕、下山健太、長光亨、供田洋 (Joint work)化学工業社 2016/09

Lectures, oral presentations, etc.

  • 海洋由来細菌KDM594株が生産するmicrococcin 類のカイコ感染症モデルにおける延命効果
    八木瑛穂; 佐藤真由; 菊地克樹; 福田隆志; 内田龍児
    第 144 回日本薬学会  2024/03
  • 深海由来微生物を用いたメラノーマ選択的抗がん活性を有する化合物の探索
    坂口舞; 菊地克樹; 岡村玲汰; 谷口亮人; 関怜子; 長井賢一郎; 安藤正史; 田中照佳; 福田隆志
    第 144 回日本薬学会  2024/03
  • Amphotericin B 活性増強物質 nectriatide 誘導体の合成と活性評価
    長井 賢一郎; 小林 啓介; 三宅 良介; 佐藤 雪乃; 関 怜子; 福田 隆志; 八木 瑛穂; 内田 龍児; 大城 太一; 供田 洋
    第 144 回日本薬学会  2024/03
  • 深海由来微生物 Bacillus licheniformis KDM612 を用いた新規 seriniquinone 配糖体の作製  [Not invited]
    岡村玲汰; 菊地克樹; 谷口亮人; 関怜子; 長井賢一郎; 大手聡; 大城太一; 安藤正史; 田中照佳; 福田隆志
    第 144 回日本薬学会  2024/03
  • かまぼこ製造時の廃棄物「水さらし液」は血中GLP-1およびインスリン量を制御することにより血糖値上昇を抑制する
    八田日和; 田中照佳; 池田くる美; 藤井有希; 福田隆志; 安藤正史
    農芸化学会  2024/03
  • 霞ヶ浦産シラウオに関する研究-Ⅱ-鮮度変化に対する曳き網時間の影響-
    安藤正史; 松下顕大; 伊藤一郎; 今泉健作; 鈴木周也; 田中照佳; 福田隆志
    令和 6 年度日本水産学会  2024/03
  • かまぼこ製造時の廃棄物「水さらし液」の血糖値上昇抑制作用  [Not invited]
    八田日和; 池田くる美; 藤井有希; 田中照佳; 福田隆志; 安藤正史
    日本栄養学会  2023/11
  • マウスモデル系を用いたエビタンパク質の経皮感作能の解析  [Not invited]
    長田理暉; 染谷采鈴; 田中照佳; 安藤正史; 福田隆志; 森山達哉
    日本栄養学会  2023/11
  • 抗真菌薬amphotericin B活性増強剤nectriatideの機能解析  [Not invited]
    小林啓介; 長井賢一郎; 三宅良介; 西村慎一; 福田隆志; 供田洋; 大城太一
    第 65 回天然有機化合物討論会  2023/09
  • 海洋微生物を利用した MONOTORI 研究  [Not invited]
    福田隆志
    日本生薬学会第69回年会  2023/09
  • Bifidobacterium dentiumのMVsが示すJurkat細胞の増殖抑制効果
    前田 瑞歩; 入江 健太; 岡田 美玖; 福田 隆志; 川本 純; 今井 友也; 栗原 達夫; 倉田 淳志; 上垣 浩一
    第 75 回生物工学会  2023/09
  • 海洋由来放線菌 Streptomyces sp. KM77-8 株が生産する新規抗生物質に関する研究  [Not invited]
    上村萌佳; 小林啓介; 佐藤倫子; 長井賢一郎; 関怜子; 神尾道也; 福田隆志; 坪内泰志; 供田洋; 大城太一; 小林武志; 寺原猛
    第 23 回マリンバイオテクノロジー学会  2023/05
  • 岡村玲汰; 谷口亮人; 長井賢一郎; 関怜子; 安藤正史; 田中照佳; 福田隆志
    第 23 回マリンバイオテクノロジー学会  2023/05
  • 福田隆志
    第 23 回マリンバイオテクノロジー学会  2023/05
  • 八木 瑛穂; 佐藤 真由; 菊地 克樹; 福田 隆志; 内田 龍児
    第 23 回マリンバイオテクノロジー学会  2023/05
  • 海洋資源に着目した抗がん活性物質の探索  [Not invited]
    田中 光樹; 長井 賢一朗; 寺原 猛; 安藤正史; 塚正 泰之; 田中 照佳; 福田 隆志
    第 143 回日本薬学会  2023/03
  • Seriniquinone 構造変換体に関する研究  [Not invited]
    石田 晃平; 大河内 瞳; 倉田 淳志; 安藤 正史; 塚正 泰之; 田中 照佳; 福田 隆志
    第 143 回日本薬学会  2023/03
  • 霞ヶ浦産シラウオに関する研究 – I – 冷蔵化における各種鮮度指標の変化-  [Not invited]
    安藤正史; 久保勇人; 伊藤一郎; 今泉健作; 福田隆志; 塚正泰之
    令和 5 年度日本水産学会  2023/03
  • Bifidobacterium dentiumが生産するMVsの特性  [Not invited]
    前田 瑞歩; 小西 莉子; 入江 健太; 岡田 美玖; 福田 隆志; 川本 純; 今井 友也; 栗原 達夫; 倉田 淳志; 上垣 浩
    第74回日本生物工学会大会  2022/10
  • 真菌 Talaromyces cellulolyticus BF-0307 株が生産する新規 SOAT 阻害剤 celludinone 類に関する研究  [Not invited]
    関 怜子; 大城 太一; 福田 隆志; 内田 龍児; 供田 洋
    第 63 回天然有機化合物討論会  2022/09
  • Tanzawaic acid A の全合成  [Not invited]
    田中 虎太郎; 李 大葵; 福田 隆志; 内田 龍児; 供田 洋; 長光 亨
    第 142 回日本薬学会  2022/03
  • メラノーマ選択的抗がん活性化合物 seriniquinone の微生物変換を利用した構造変換体の作成に関する研究  [Not invited]
    石田 晃平; 田中 光樹; 長井 賢一朗; 寺原 猛; 安藤 正史; 塚正 泰之; 福田 隆志
    第 142 回日本薬学会  2022/03
  • 真菌由来 nectriatide の合成中間体が示す抗真菌剤 amphotericin B 活性 増強作用  [Not invited]
    佐藤 雪乃; 長井 賢一郎; 小林 啓介; 福田 隆志; 供田 洋
    第140回 日本薬学会  2020/03
  • マダイの短期熟成に関する研究-1  [Not invited]
    塚正泰之; 仲辻晃大; 松岡大地; 西野美響; 福田隆志; 安藤正史
    令和3年度日本水産学会秋季大会  2019/09
  • アムホテリシンB活性増強物質 nectriatideに関する研究
    長井賢一郎; 福田隆志; 小林啓介; 八木瑛穂; 内田龍児; 供田洋
    第 139 回日本薬学会  2019/03
  • 黄色ブドウ球菌の免疫抵抗性因子 staphyloxanthin の生合成阻害剤に関する研究  [Not invited]
    小林啓介; 出町歩; 福田隆志; 小山信裕; 供田洋
    第 139 回日本薬学会  2019/03
  • 完全養殖クロマグロの水銀に関する研究-XII
    安藤正史; 朴世朱; 箱谷一樹; 福田隆志; 塚正泰之
    平成 31 年度日本水産学会  2019/03
  • 海洋微生物に新たな物質生産を促す  [Not invited]
    福田隆志; 安藤正史; 塚正泰之
    第 20 回マリンバイオロジー学会  2018/05
  • Discovery of marine microbial products  [Not invited]
    Fukuda Takashi
    VIIIth US-Japan Seminar on Marine Natural Products in Hawaii, US  2016/11
  • Yellow pigment inhibitors from Marine environments  [Not invited]
    Fukuda Takashi
    Gordone Research Conferences  2016
  • New compounds produced by the fungus Aspergillus nidulans BF0142 isolated from hot spring-derived soil  [Not invited]
    Fukuda Takashi
    International Symposium on Natural Products for the Future Tokushoma  2016
  • Discovering New Biologically active compounds from Marine-derived microorganism  [Not invited]
    Fukuda Takashi
    The 8th Korea-Japan Chemical Biology Symposium.  2016/01
  • Biological activity of citridone A and its derivatives  [Not invited]
    Fukuda Takashi
    Society for Industrial Microbiology and Biotechnology  2015/01
  • Graphiumins, New Thiodiketopiperazines from Marine-derived Fungus Graphium sp. OPMF00224.  [Not invited]
    Fukuda Takashi
    The 7th Korea-Japan Chemical Biology Symposium  2014/01
  • Discovering New Bioactive Products from Terrestrial and Marine Microorganisms  [Not invited]
    Fukuda Takashi
    The 6th Korea-Japan Chemical Biology Symposium  2012/01
  • New Di-phenolics from Fruit Bodies of Tylopilus eximius  [Not invited]
    Fukuda Takashi
    52nd Annual Meeting of The American Society of Pharmacognosy  2011
  • Chemical study of citridones and citrinamides produced by Penicillium sp. FKI-1938  [Not invited]
    Fukuda Takashi
    Bergey’s International Society for Microbial Systematics  2011
  • Chemical study of secondary metabolites produced by Penicillium citrinum FKI-1938 isolated at Ishigakijima  [Not invited]
    Fukuda Takashi
    US-JAPAN Seminar  2011
  • Chemical Study of Citridones, Potentiatores of Anti-Fungal Miconazole Activity  [Not invited]
    Fukuda Takashi
    IUPAC ICOB-5 & ISCNP-25  2006
  • Novel fropyridinones, potentiatores of miconazole anti-Candida albicans activity  [Not invited]
    Fukuda Takashi
    8th Society for Industrial Microbiology GMBIM/BMP  2004

Research Themes

  • 日本学術振興会:科学研究費助成事業
    Date (from‐to) : 2024/04 -2025/03 
    Author : 坪内 泰志; 仁木 満美子; 福田 隆志; 八代 正和; 瀬良 知央; 金子 幸弘
  • スイカから抽出したエキスの機能性成分の単離分析
    株式会社萩原農場:
    Date (from‐to) : 2024/05 -2025/03
  • Japan Society for the Promotion of Science:Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2021/04 -2025/03 
    Author : 坪内 泰志; 仁木 満美子; 福田 隆志; 八代 正和; 瀬良 知央; 金子 幸弘
     
    コロナ禍による制限実施もあり、予定していた計画より多少の遅延が認められる。 令和3年度では研究課題対象である抗スキルス胃癌活性を呈する深海・海洋性放線菌32株のうち4株に対して、活性物質の精製法確立および、生産菌ゲノム解析を行なった。4株が生産する活性候補分子は非タンパク質性の有機化合物であることから、C18固相抽出、酢酸エチルを用いた液液分画、C18系Preparative HPLCシステムを用い、ほぼ単一ピークになるまで精製するに至った。活性評価には弊学附属病院で樹立した培養細胞(正常線維芽細胞及びスキルス胃癌細胞株7株)を用い、精製各段階での活性の追跡、および比活性算出に用いた。同活性化合物群を対象とするOrbitrap-MSを用いた精密質量分析では、それらのm/zが500~800の範囲内に収まることから、分析対象とする4活性化合物は低中分子化合物であることが明らかとなった。生産菌ゲノム解析では、long read dataにはONT社MinIONシステム、short read dataにはIllumina社NovaSeqの2プラットフォームを採用し、得られた各データからopen source codeによるバイオインフォマティクス手法でwhole genome配列を構築することに成功した。決定した4株分のゲノム配列はそのゲノムサイズが6Gb~11Gbと幅広く、コードされている16S rRNA遺伝子を指標とした分析からはいずれも新種株に相当することが見出された。解析したゲノム配列は公共データベースであるDDBJに登録申請済みである(データ非公開)。
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Industrial Property Rights

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