ISHIBASHI Yasunori

Researcher Information

Degree

• Doctor(Agriculture)（Kindai University）
• Master(Agriculture)（Kindai University）

URL

http://www.nara.kindai.ac.jp/

J-Global ID

• 200901096323063228

Research Interests

• 行動制御   Stress   Early life history   Lavae and juvenile   Aquaculture   種苗生産   動物生理・代謝   環境生理学   水産増殖   Animal Physiology and Metabolism   Enviromental Physiology (including Nutritional physiology)   Aquacaltural Science   

Research Areas

• Life sciences / Marine/Aquatic life sciences
• Life sciences / Aquaculture
• Life sciences / Aquaculture
• Life sciences / Animals: biochemistry, physiology, behavioral science

Academic & Professional Experience

• 2010/04 - Today  Kindai UniversityProfessor
• 2007/04 - 2010/03  Kindai University准教授
• 2003/04 - 2007/03  Kindai University助教授
• 1996/04 - 2002/03  Kindai University
• 1996 - 2002  Kindai University,
• 1992/04 - 1995/03  Kindai University
• 1992 - 1995  Kindai University,

Association Memberships

• THE ZOOLOGICAL SOCIETY OF JAPAN   世界水産増殖学会   マリンバイオテクノロジー学会   日本水産増殖学会   日本水産学会   World Aquaculture Society   Japan Aquaculture Society   The Japanese society of Fisheries Science   

Published Papers

• 59) Nighttime Lighting Influences on the Plankton Feeding and Growth of Juvenile Pacific Bluefin Tuna, Thunnus orientalis

  Tomoki Honryo; Yoshifumi Sawada; Yasunori Ishibashi

  Animals 2023 MDPI AG 13 (19) 3128 - 3128 2023/10 [Refereed]
   

  During fingerling production of Pacific bluefin tuna (PBF) Thunnus orientalis, heavy mortality can occur immediately after juveniles are transferred from nursery tanks to sea cages; however, nighttime lighting can moderate this mortality. Additionally, various live prey aggregate due to nighttime lighting in practical sea cage culture of PBF. Here, we investigated whether the growth and viability of PBF juveniles could be improved through promoting feeding on live prey that aggregate under nighttime lighting. Two treatment groups were established using land-based tanks under constant environmental conditions, one in which the juveniles were fed live prey at night (night-feed in four replicate tanks) and the other in which juveniles were not fed during the night (control in four replicate tanks). Although the survival rate did not differ significantly between the two groups, growth was significantly improved in the night-feed group, in which 69–78% of the juveniles showed evidence of feeding during the night. Thus, nighttime lighting plays a vital role in the aggregation of various live prey. PBF juveniles consume these prey in sea cages, which promotes their growth. This may partially serve as a countermeasure against the heavy mortality observed in sea cages.
• 58) Appropriate size for transportation to sea cages for juvenile Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel)

  Tokihiko Okada; Tomoki Honryo; Masato Kawahara; Ichita Takahashi; Kou Murayama; Yasunori Ishibashi

  Aquaculture Research Wiley 52 (3) 1282 - 1290 1355-557X 2020/11 [Refereed]
  
• 57) Improving rearing performance in sea‐cage culture of Pacific bluefin tuna Thunnus orientalis juveniles (Temminck and Schlegel) using small sea cages

  Tokihiko Okada; Yuki Sakurai; Tomoki Honryo; Masato Kawahara; Yasunori Ishibashi

  Aquaculture Research Wiley 51 (7) 3017 - 3024 1355-557X 2020/07 [Refereed]
  
• 56) Effects of night-time light intensity and area of illumination in the sea cage culture of Pacific bluefin tuna (Thunnus orientalis) juveniles

  Tomoki Honryo; Tokihiko Okada; Masato Kawahara; Yoshifumi Sawada; Michio Kurata; Yasunori Ishibashi

  Aquaculture Elsevier BV 521 735046 - 735046 0044-8486 2020/05 [Refereed]
  
• 55) Blood chemistry of Pacific bluefin tuna (Thunnus orientalis) juveniles showing abnormal swimming behavior.

  Tomoki Honryo; Tokihiko Oakada; Michio Kurata; Yasunori Ishibashi; Yasuo Agawa; Yoshifumi Sawada

  Aquaculture Elsevier BV 506 355 - 358 0044-8486 2019 [Refereed]
  
• 54) Estimated time for recovery from transportation stress and starvation in juvenile Pacific bluefin tuna Thunnus orientalis

  Tomoki Honryo; Tokihiko Oakada; Masato Kawahara; Michio Kurata; Yasuo Agawa; Yoshifumi Sawada; Shigeru Miyashita; Kenji Takii; Yasunori Ishibashi

  AQUACULTURE ELSEVIER SCIENCE BV 484 175 - 183 0044-8486 2018/02 [Refereed]
   

  It is important to understand the physiological stress responses in juvenile Pacific bluefin tuna (PBF), Thunnus orientalis, when transporting them from land-based tanks to sea cages, to develop countermeasures against mass mortality and enhance survival. We investigated the estimated time required to recover from transportation stress in PBF juveniles (32 and 35 days post hatch (DPH)) transported over two different distances (Exp 1; long distance (15.2 km), Exp 2; short distance (2.3 km)). Additionally, we examined the effects of simulated transport in fish that were transported from tank-to-tank, as well as starvation tolerance to determine the causes of stress responses during transportation. Whole-body cortisol levels and HIF-1 alpha expression levels in gills increased from pre-transport levels shortly after juveniles were released into the sea cage in Exp 1, but recovered within 24 h. Simultaneously, whole-body glucose levels fell over the 48 h after transport. Gene expression of the Hsp70 family in the liver was upregulated 48 h after fish were released into the sea cages in Exp 1, and was significantly upregulated after 48 h in Exp 2. These results indicated that 72 h were required for PBF juveniles to recover from transportation to sea cages. The starvation experiment suggested that PBF juveniles were significantly affected after 2 days of fasting and that the environmental conditions in the sea cage were the main factors resulting in a decrease in whole-body glucose levels and upregulation of Hsp70-family gene expression. Furthermore, changes of proximate compositions indicated that PBF juveniles consumed crude lipids and proteins, which were significantly affected by fasting for 1-2 days. We suggested that the first 3 days after stocking are a critical period for recovery from transportation stress and it is necessary to develop appropriate management techniques to alleviate the high mortality caused by these stress responses.
• 53) Effects of photoperiod and night-time aeration rate on swim bladder inflation and survival in Pacific bluefin tuna, Thunnus orientalis (Temminck & Schlegel), larvae

  Michio Kurata; Yoshiki Tamura; Tomoki Honryo; Yasunori Ishibashi; Yoshifumi Sawada

  AQUACULTURE RESEARCH WILEY 48 (8) 4486 - 4502 1355-557X 2017/08 [Refereed]
   

  Success of swim bladder inflation (SBI) is crucial for early survival of Pacific bluefin tuna (PBF) larvae, because it reduces larval sinking death by enhancing buoyancy. In Experiment 1, we examined the effect of photoperiod on SBI and survival in PBF larvae by comparing photoperiods of 9L: 15D (9L), 14L: 10D (14L: natural photoperiod), 19L: 5D (19L) and 24L: 0D (24L) during 2-10days post hatch (dph). In Experiment 2, the combined effects of photoperiod (24L and 14L) and nighttime aeration rate (enhanced night-time aeration: ENA of 1300mLmin(-1) as a countermeasure for sinking death and 130mLmin(-1)) on the survival and SBI were also examined during 2-10 dph. Moreover, in Experiment 3 the effect of photoperiod on vertical distribution of larvae in night-time was examined on 3-5 dph. Photoperiod of 24L in Experiment 1 significantly inhibited SBI compared with 14L and 19L; nevertheless, it significantly improved survival compared with other photoperiods with a dark period. On the other hand, the shortened light period (9L) showed significantly reduced SBI and also survival. In Experiment 2, the countermeasure for sinking death of ENA under 24L did not further improve the survival; rather it tended to reduce the survival. In Experiment 3, larvae distributed less in the bottom layer in 24L than in 14L, suggesting the reducing effect of 24L on sinking death. The results indicate that 24L without ENA is suitable for survival which is the most serious problem in PBF larviculture.
• 52) Sequence analysis and expression patterns of opsin genes in the longtooth grouper Epinephelus bruneus

  Taro Matsumoto; Yasunori Ishibashi

  FISHERIES SCIENCE SPRINGER JAPAN KK 82 (1) 17 - 27 0919-9268 2016/01 [Refereed]
   

  Here, to evaluate ontogenic changes in visual sensitivity in juvenile longtooth grouper Epinephelus bruneus, we investigated opsin gene sequences and expression in larvae and juveniles. The longtooth grouper has 10 opsin genes: Rh1, five Rh2 genes (Rh2A1-1, Rh2A1-2, Rh2A2, g6738, and g6740), SWS1, two SWS2 genes (SWS2A1 and SWS2A2), and LWS. To date, five or six Rh2 opsin genes have been reported in perciform fishes. Differential opsin gene expression was examined by RT-PCR; the results suggested that switching from SWS1 to SWS2 occurs during early development, and that there is an absence of LWS expression between 14 and 40 days post-hatch. These ontogenetic changes in opsin gene expression may correspond to different requirements at different life stages.
• 51) Electroretinographic evaluation and SWS1 opsin gene expression in the vision of juvenile longtooth grouper Epinephelus bruneus ( Bloch, 1793)

  Taro Matsumoto; Yasunori Ishibashi

  MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY TAYLOR & FRANCIS LTD 48 (6) 381 - 395 1023-6244 2015/11 [Refereed]
   

  Electroretinogram (ERG) recordings and short-wave sensitive 1 (SWS1: ultraviolet sensitive) opsin gene expression data were obtained to investigate the spectral sensitivity and scotopic luminous sensitivity of juvenile longtooth groupers Epinephelus bruneus. The ERG showed broad spectral sensitivity (350-600nm; (max)=560nm approx.) in the photopic condition. The scotopic vision was maximally sensitive to green light ((max)=500nm approx.). SWS1 opsin gene expression was measured in the eyes using the reverse transcription-polymerase chain reaction method (RT-PCR). This indicated that the ultraviolet sensitivity in the photopic spectral response curve (360nm approx.) was attributable to SWS1 opsin absorption. The contribution of the -band cannot rule out. Scotopic luminous sensitivity in this species similar to that in other coastal fishes and higher than that in pelagic fishes. These results suggest that the vision of a juvenile longtooth grouper is adapted to conditions in shallow coastal waters.
• 50) Influence of swimbladder inflation failure on mortality, growth and lordotic deformity in Pacific bluefin tuna, Thunnus orientalis, (Temminck & Schlegel) postflexion larvae and juveniles

  Michio Kurata; Yasunori Ishibashi; Manabu Seoka; Tomoki Honryo; Shigeru Katayama; Hiromu Fukuda; Kenji Takii; Hidemi Kumai; Shigeru Miyashita; Yoshifumi Sawada

  AQUACULTURE RESEARCH WILEY-BLACKWELL 46 (6) 1469 - 1479 1355-557X 2015/06 [Refereed]
   

  This study examined the influence of swimbladder inflation (SBI) failure on mortality (Experiment 1), lordotic deformity (Experiment 2) and growth (Experiment 1, 2) in Pacific bluefin tuna, Thunnus orientalis, postflexion larvae and juveniles by producing larvae lacking inflated swimbladders. Experiment 1 was conducted for postflexion larvae to juveniles (from 18 to 30days-post-hatch; dph). Mortality was not significantly different between the fish with (WIS) and without (WOIS) inflated swimbladders. Standard length (SL) and body weight (BW) were significantly smaller in WOIS than WIS. Moreover, the SBI was found in WOIS after postflexion stage. In Experiment 2, two examination trials were conducted on SBI, vertebral deformity and growth for juvenile stage. Lordotic deformities were found neither in the WOIS nor in the WIS. Although SL and BW were significantly smaller in WOIS than WIS at 22dph, after 37dph no significant differences were found between them. The results of Experiment 1 and 2 indicate that SBI failure causes growth retardation until juveniles of 30dph; however, it does not cause growth retardation in juveniles after 37dph and mortality of postflexion larvae and juveniles. Moreover, SBI failure did not cause lordotic deformity in PBT differently from many other fish.
• 49) Timing to promote initial swimbladder inflation by surface film removal in Pacific bluefin tuna, Thunnus orientalis (Temminck and Schlegel), larvae

  Michio Kurata; Manabu Seoka; Yasunori Ishibashi; Tomoki Honryo; Shigeru Katayama; Kenji Takii; Hidemi Kumai; Shigeru Miyashita; Yoshifumi Sawada

  AQUACULTURE RESEARCH WILEY-BLACKWELL 46 (5) 1222 - 1232 1355-557X 2015/05 [Refereed]
   

  This study investigated the optimal timing of day to promote initial swimbladder inflation (ISI) for improved Pacific bluefin tuna (PBT), Thunnus orientalis, larval survival. Larval swimbladder inflation frequency was compared based on three experiments using different time schemes of surface film removal (SFR) from 3 to 9days post hatch (dph). SFR was conducted from 05:00 to 19:00hours (light period: S.5-19), 19:00 to 05:00hours (dark period: S.19-5), 08:00 to 19:00hours (S.8-19) and the entire day (S.24) in Experiment 1; from 08:00 to 19:00hours (S.8-19-E2), 08:00 to 13:00hours (S.8-13), 13:00 to 19:00hours (S.13-19) in Experiment 2; and from 13:00 to 16:00hours (S.13-16), 16:00 to 19:00hours (S.16-19), 18:00-19:00hours (S.18-19) in Experiment 3. The swimbladder inflation frequency at the experiment termination (9dph) was significantly higher (P<0.001) in S.24 (91.1 +/- 5.7%), S.5-19 (92.2 +/- 5.1%) and S.8-19 (93.3 +/- 3.4%) than in S.19-5 (11.1 +/- 5.1%) in Experiment 1, and remarkably higher in S.8-19-E2 (81.7%) and S.13-19 (88.3%) than in S.8-13 (0.0%) in Experiment 2, and significantly higher (P<0.001) in S.16-19 (84.4 +/- 5.1%) and S.18-19 (70.0 +/- 12.0%) than in S.13-16 (7.8 +/- 3.9%) in Experiment 3. These results suggest that the optimal timing to promote larval ISI by SFR is a few hours before the end of light period (16:00-19:00hours) from 3 to 9dph.
• 48) Effect of tank wall colour and pattern on the survival rate of juvenile Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel) during ship transportation

  Tokihiko Okada; Masahiro Nakatani; Yoshifumi Sawada; Shigeru Miyashita; Hidemi Kumai; Yasunori Ishibashi

  AQUACULTURE RESEARCH WILEY-BLACKWELL 46 (2) 446 - 452 1355-557X 2015/02 [Refereed]
   

  Juvenile Pacific bluefin tuna (Thunnus orientalis; PBT) often experience high mortality during ship transportation. This study investigated whether the addition of colours or patterns to the walls of tanks affected survival rate. In the first experiment, three colours and lattice patterns were tested: dark blue single-colour, red single-colour, and red-blue lattice pattern. Fish in all tanks exhibited abnormal behaviours when sunlight entered the tanks between 0800 and 1000hours, but mortality only increased in the single-coloured tanks as a result of collision with the tank walls. In the second experiment, four colours and patterns were tested: dark blue single-colour, red-blue lattice pattern, red-blue lattice pattern with shade sheet and red-green lattice pattern with shade sheet. Again, we visually observed that fish in all treatment groups exhibited abnormal behaviour when sunlight entered the tanks, but there were no collision deaths in the lattice-patterned tanks and survival in this group was significantly higher than in the single-coloured tanks. Thus, the use of a high-contrast colour pattern can prevent mass death of juvenile PBT during ship transportation.
• 47) Opsin gene analysis in the cultured kawakawa Euthynnus affinis

  Taro Matsumoto; Yasuo Agawa; Tokihiko Okada; Yoshifumi Sawada; Yasunori Ishibashi

  Aquaculture Science 63 (2) 179 - 189 2015 [Refereed]
  
• 46) Effect of Illuminance Transition Rate on Survival of Juvenile Pacific Bluefin Tuna, Thunnus orientalis

  Tomoki Honryo; Michio Kurata; Tokihiko Okada; Yoshifumi Sawada; Yasunori Ishibashi

  JOURNAL OF THE WORLD AQUACULTURE SOCIETY WILEY-BLACKWELL 45 (5) 523 - 531 0893-8849 2014/10 [Refereed]
   

  To investigate the reason for the high mortality of cultured juvenile Pacific bluefin tuna (PBT), especially during land-based culture, we examined the effect of the rate of light-intensity change during the dawn period. Juvenile PBT kept in a natural light environment (control) were compared with those exposed to an artificially slowed change in light intensity during the dawn period (test group). For the test group, lamps, which were connected to automatic timers and placed above the rearing tank, were switched on from 0345 to 0515 h at 15-min intervals. After 9 d, the survival rate of the test and control group was identical. Between Day 1 and Day 6, whole-body cortisol levels were not different between groups. However, whole-body glucose levels in the test group were significantly higher than those in the control group. Both groups displayed a diel rhythm in plasma cortisol concentration that peaked at 0300-0600 h. These results suggest that slowing the increase in light intensity during the dawn period did not improve survival and that low light intensity itself induces high mortality in cultured PBT juveniles.
• 45) Optimal periods of night-time lighting in the sea cage culture of Pacific bluefin tuna, Thunnus orientalis, juvenile (Temminck and Schlegel)

  Tomoki Honryo; Tokihiko Okada; Michio Kurata; Toshihiro Tamura; Yasunori Ishibashi

  AQUACULTURE RESEARCH WILEY-BLACKWELL 45 (7) 1109 - 1115 1355-557X 2014/06 [Refereed]
   

  We have previously reported that night-time lighting prevents the mass death of Pacific bluefin tuna (PBT) juvenile due to collision and/or contact with the walls of sea cages, immediately after transfer to the cages, and that night-time lighting does not negatively impact fish stress levels. However, the period of night-time lighting should be limited to minimize negative impacts on the surrounding environment and aid management. Therefore, we investigated the optimal period of night-time lighting by evaluating the whole-body cortisol and glucose levels as stress parameters, growth performance and survival of PBT juvenile in four cages with different periods of night-time lighting (i.e. unlit, 4-day, 8-day and 12-day lighting). The results showed that almost all fish were died 1day after transfer to the unlit cage. In comparison, the other groups (4-day, 8-day and 12-day lighting) had high survival rates (92.5-96.0%) without significant difference. However, in the 4-day-lighting group, an obvious stress response was recorded on day 5, and growth performance was significantly lower. In the 8-day-lighting group, whole-body cortisol levels were slightly elevated on day 9; however, significantly elevation was not recorded on day 12. These results indicate that the recommended lighting period of night-time lighting in sea cages is 8-12days.
• 44) Influence of initial swimbladder inflation failure on survival of Pacific bluefin tuna, Thunnus orientalis (Temminck and Schlegel), larvae

  Michio Kurata; Yasunori Ishibashi; Kenji Takii; Hidemi Kumai; Shigeru Miyashita; Yoshifumi Sawada

  AQUACULTURE RESEARCH WILEY-BLACKWELL 45 (5) 882 - 892 1355-557X 2014/04 [Refereed]
   

  This study investigated the influence of initial swimbladder inflation (ISI) failure on survival of Pacific bluefin tuna (PBT), Thunnus orientalis, larvae to prevent mass mortality due to sinking death. In Experiment 1, swimbladder inflation frequency and survival within an ISI promoted (PS) group, for which surface film of rearing water was removed, and a group without ISI promotion (NPS) were compared in 20 and 30kL tanks. The PS group demonstrated significantly higher swimbladder inflation frequency and increased survival than the NPS group within 20kL tanks at 9days post hatching (dph). Similar tendencies were observed within 30kL tanks. In Experiment 2, larval distribution and swimbladder inflation frequency in the night-time were examined through larval sampling within the upper and middle layers of the water column and tank bottom within 1.6 and 30kL tanks at 5dph. Larvae at tank bottom had higher distributional density and significantly lower swimbladder inflation frequency than those distributed in the upper and middle layers within 1.6kL tanks. Similar tendencies were observed within 30kL tanks. Results of this study suggest the improvement of larval survival due to prevention of sinking death through ISI promotion in mass-scale PBT larviculture.
• 43) The cause of death of juvenile Pacific bluefin tuna (Thunnus orientalis) reared in sea net cages

  Tokihiko Okada; Tomoki Honryo; Yoshifumi Sawada; Yasuo Agawa; Shigeru Miyashita; Yasunori Ishibashi

  AQUACULTURAL ENGINEERING ELSEVIER SCI LTD 59 23 - 25 0144-8609 2014/03 [Refereed]
   

  Although the survival rate of juvenile Pacific bluefin tuna Thunnus orientalis (PBT) during 30 days after stocking in sea net cages has been as low as approximately 50%, the reason for this high mortality is not clear. The dead fish were collected and counted during 30 days after stocking in a sea net cage, and the growth performance, stomach contents, and bone injury were investigated. Nearly half (47.9%) had died within the first 4 days. The total length and body weight of the dead fish were significantly smaller than those of the live fish, and the weight of the dead fish did not increase during the first 7 days. Approximately one-third of the dead fish (21.9-42.9% per day) had ingested inorganic matter such as wood or Styrofoam waste during the first 7 days, and 25.0-45.0% of the dead fish sampled per day showed poor growth. The daily ingestion rate of inorganic matter decreased dramatically from the eighth day, when the daily mortality rate decreased. The percentage of dead fish with bone injuries due to collision or contact was low (less than 15%). These results suggest that one of the causes of high mortality during several days after stocking in a sea cage was accidental ingestion of inorganic matter by juvenile PBT. Improvement in feeding method can increase the survival rate of juveniles in sea net cages. (C) 2014 Elsevier B.V. All rights reserved.
• 42) Effects of light wavelength on growth and survival rate in juvenile Pacific bluefin tuna, Thunnus orientalis

  Yoshiki Tsutsumi; Taro Matsumoto; Tomoki Honryo; Yasuo Agawa; Yoshifumi Sawada; Yasunori Ishibashi

  ENVIRONMENTAL BIOLOGY OF FISHES SPRINGER 97 (1) 53 - 58 0378-1909 2014/01 [Refereed]
   

  The spectral sensitivity of the fish and the suitable light wavelength range for survival and growth performance of juvenile Pacific bluefin tuna (PBT) were investigated. The spectral sensitivity peak of PBT under photopic condition was observed between 449 and 503 nm, which corresponded to their natural habitat. The fish were reared in tanks irradiated continuously with 4 kinds of light emitting diodes (LEDs). The maximum wavelength of LEDs used for the rearing experiment were 460 nm (blue), 520 nm (green), 630 nm (red), and 450-680 nm (white). There was no notable difference in survival rate among fish in the four LED groups. However, the growth of juvenile PBT was lesser under red light compared to the green and white light wavelengths. These results suggest that PBT juveniles have low sensitivity to red light because the fish are rarely exposed to the red light wavelengths under natural ocean conditions. Thus, low sensitivity to red light negatively influenced the feeding behavior and growth of PBT juveniles.
• 41) Effects of feeding conditions and size differences on aggressive behaviour and cannibalism in the Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel) larvae

  Yasunori Ishibashi; Takahisa Miki; Yoshifumi Sawada; Michio Kurata

  AQUACULTURE RESEARCH WILEY 45 (1) 45 - 53 1355-557X 2013/12 [Refereed]
   

  We examined the effects of feeding conditions and size differences on the aggressive behaviour and cannibalism in Pacific bluefin tuna (PBT) Thunnus orientalis larvae. In a 24h experiment, restricted feeding alone was found to remarkably increase the frequency of aggressive behaviour, which was further elevated by differences in fish size. In a 4-day rearing experiment, while aggressive behaviour was increased by restricted feeding alone, the frequency of cannibalism did not change significantly. Although the frequency of aggressive behaviour did not increase with difference in size factor, small fish in this group gradually tended to die over 4-day period. In the restricted feeding and size difference group, large fish were observed to bite the small fish, and almost all the small fish died on the day after the start of the experiment. These results suggest that the aggressive behaviour of PBT larvae is chiefly increased by the shortage of live food; however, deaths related to cannibalism mainly occurred in small larvae and rapidly increased with food restriction and differences in fish size.
• 40) Effects of tank wall pattern on survival, bone injury rate, and stress response of juvenile Pacific bluefin tuna, Thunnus orientalis

  Yasunori Ishibashi; Tatsuya Izumi; Michio Kurata; Tokihiko Okada

  AQUACULTURAL ENGINEERING ELSEVIER SCI LTD 56 13 - 17 0144-8609 2013/09 [Refereed]
   

  Juvenile Pacific bluefin tuna (PBT), Thunnus orientalis, aged 30 or more days post hatching (>= 5 cm total length) are at high risk of mortality through contact or collision with tank walls. In this study, survival rates of PBT reared under three tank wall treatments (polka dot pattern, lattice pattern, and dark-green single color (control) were investigated. White tape was attached to the tank's surface to form either a polka dot or a lattice pattern. Fish were then reared in the tanks for 9 days. Fish survival rates in the lattice patterned and polka-dot patterned tanks were higher than that in the control tank. Bone and parasphenoid injury rates in the control group were significantly higher than those in the polka dot and lattice patterned treatment groups. The plasma cortisol content of juvenile PBT reared in the control group was higher than for fish reared in patterned tanks. These results suggest that juvenile PBT mortality from contact or collision with tank walls was reduced when wall visibility was improved by creating patterns on the tank walls. (C) 2013 Elsevier B.V. All rights reserved.
• 39)Effects of night-time light intensity on the survival rate and stress responses in juvenile Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel)

  Tomoki Honryo; Michio Kurata; Tokihiko Okada; Yasunori Ishibashi

  Aquaculture Research 44 (7) 1058 - 1065 1355-557X 2013/06 [Refereed]
   

  Land-based cultured juvenile Pacific bluefin tuna Thunnus orientalis (PBT) have high mortality rates due to collisions or contacts with tank walls after about 30 days of hatching. To determine the effect of night-time lighting on their survival, juvenile PBT were reared under four different night-time light intensities (0, 5, 15 and 150 lx) for 9 days, followed by a 3-day observation period. High-intensity, night-time lighting (150 lx) significantly improved the survival rate (75.8% P < 0.001) compared with the unlit control group (0 lx, 64.3%). The survival rate in the high-intensity group decreased after the end of the lighting period. Lighting did not influence whole-body cortisol levels, glucose levels, or diel changes in plasma cortisol levels. In contrast, the survival rates of fish exposed to light intensities between 5 and 15 lx were slightly lower than that of the unlit control group. These results suggest that providing night-time lighting of 150 lx or higher is an effective method for reducing the mortality of cultured PBT. © 2013 John Wiley & Sons Ltd.
• 38) Suitable Photoperiod and Light Intensity of Laboratory-reared Juvenile Pacific Bluefin Tuna Thunnus orientalis

  Yasunori Ishibashi; Tomoki Honryo; Shigeru Miyashita; Seiji Oda

  Aquaculture Science 61 399 - 402 2013 [Refereed]
  
• 37) Novel method for improving the antioxidative properties of fish meat by direct injection of sodium l-ascorbate into the blood vessels of live fish

  Yasuyuki Tsukamasa; Keisuke Kato; Bimol Chandra Roy; Yasunori Ishibashi; Toru Kobayashi; Tomohiro Itoh; Masashi Ando

  Fisheries Science 79 (2) 349 - 355 0919-9268 2013 [Refereed]
   

  The color of the red muscle of pelagic fish is used as an indicator of freshness, and sustaining a bright red color is important for maintaining the commercial value of pelagic fish. Feeding fish with antioxidant compounds can delay metmyoglobin (metMb) formation, but the process requires long-term feeding. Live cultured Japanese horse mackerel Trachurus japonicus were used in this study. After anesthetization, 2 ml of blood was drawn from blood vessels, and a parenteral solution including an antioxidant compound was injected. Browning, metMb formation, and lipid oxidation of dark muscle during chilled storage were delayed by injecting sodium l-ascorbate as an antioxidant compound in the blood vessels of live fish. © 2013 The Japanese Society of Fisheries Science.
• 36) Visual spectral sensitivity of photopic juvenile Pacific bluefin tuna (Thunnus orientalis)

  Taro Matsumoto; Tokihiko Okada; Yoshifumi Sawada; Yasunori Ishibashi

  FISH PHYSIOLOGY AND BIOCHEMISTRY SPRINGER 38 (4) 911 - 917 0920-1742 2012/08 [Refereed]
   

  Although Pacific bluefin tuna is a species that relies on vision, its photopic visual function is not well known; we therefore recorded electroretinograms to investigate photopic spectral sensitivity in juveniles of this species (49-81 days post-hatch; standard length 74-223 mm). The peak spectral sensitivity wavelength was 505 nm. We estimated that two (lambda(max) = 512-515 nm and 423-436 nm) or three (lambda(max) = 512-515 nm, 423-436 nm, and 473 nm) types of cone visual pigments contribute to photopic vision; these spectral sensitivities are adapted to surface water habitats in clear ocean and coastal water.
• 35) Promotion of initial swimbladder inflation in Pacific bluefin tuna, Thunnus orientalis (Temminck and Schlegel), larvae

  Michio Kurata; Manabu Seoka; Yoshizumi Nakagawa; Yasunori Ishibashi; Hidemi Kumai; Yoshifumi Sawada

  AQUACULTURE RESEARCH WILEY-BLACKWELL 43 (9) 1296 - 1305 1355-557X 2012/08 [Refereed]
   

  Initial swimbladder inflation (ISI) of Pacific bluefin tuna (PBT), Thunnus orientalis, larvae was studied to increase the survival of cultured larvae. Experiment 1 was conducted to explore promotion and inhibition of ISI under different water surface conditions; including the use of surface skimmer to remove autogenous surface substances (SS), covering the water surface with liquid-paraffin-layer (LP) and oil film (OF), and a control (non-treatment, NT). Significantly higher inflation frequency was observed in SS (62.2%) than NT (11.9%), LP (2.7%) and OF (3.9%). This indicates that ISI in PBT larvae can be promoted by removal of surface substances on rearing water which inhibit larval air gulping. Experiment 2 aimed to elucidate proper day of larval age to start skimming for promoting ISI with four different periods of oil film removal: from 3 to 8 (SF3D), 4 to 8 (SF4D), 5 to 8 (SF5D), 6 to 8 (SF6D) days-post-hatch (dph). Significant improvement in ISI frequency was observed in SF3D (80.2%) but the frequency was very poor in SF4D, SF5D, and SF6D (17.87.5%). This implies the need of oil film removal without missing a narrow window, 1 day of 3 dph, to promote ISI in practical PBT larviculture.
• 34) Changes in the scotopic vision of juvenile Pacific bluefin tuna (Thunnus orientalis) with growth

  Taro Matsumoto; Tokihiko Okada; Yoshifumi Sawada; Yasunori Ishibashi

  FISH PHYSIOLOGY AND BIOCHEMISTRY SPRINGER 37 (3) 693 - 700 0920-1742 2011/09 [Refereed]
   

  In cultured juvenile Pacific bluefin tuna (Thunnus orientalis), reducing the mass deaths caused by collision or contact with tank or net walls at night is a priority for seedling production. Pacific bluefin tuna is a visually dependant species, although its scotopic vision is poor. We recorded electroretinograms to investigate the visual function with growth in the dark-adapted eyes of juvenile Pacific bluefin tuna. Peak wavelengths of spectral sensitivity [38-62 days posthatch (dph), 77-167 mm standard length (SL)] were observed between 474 and 494 nm. Visual light sensitivity has a tendency to increase slightly with growth at 28-64 dph in individuals that measured 29-175 mm SL. However, visual temporal resolution did not significantly increase with growth at 38-62 days dph in individuals that measured 77-167 mm SL. These results suggest that the mass death continues between 28 and 64 dph because of low visual function and increasing swimming speed with growth.
• 33) Aggressive behaviour and cannibalism in greater amberjack, Seriola dumerili: effects of stocking density, feeding conditions and size differences

  Takahisa Miki; Hiromu Nakatsukasa; Norihide Takahashi; Osamu Murata; Yasunori Ishibashi

  AQUACULTURE RESEARCH WILEY-BLACKWELL 42 (9) 1339 - 1349 1355-557X 2011/08 [Refereed]
   

  Aggressive behaviour, assessed by 24-h observations, and survival rates, determined in a 2-week rearing experiment, were examined in greater amberjack, Serbia dumerili, subjected to single and multiple factors (high density, restricted feeding and diverse size). Aggressive behaviour frequency in the group subjected to both restricted feeding and size diversity (FS) was significantly higher than that in the other groups (Tukey, n = 4, P < 0.05). In the FS group, aggressive behaviour increased with the starvation time from 19 days post hatching (dph) to 23 dph (two-way analysis of variance, Tukey, n = 4, P < 0.05), but decreased gradually thereafter to 46 dph. In the rearing experiment, the survival rates for the diverse size and FS groups were lower than those of the control or restricted feeding groups (Kaplan-Meier, log-rank test, n = 100, P < 0.01). Daily mortality in all groups was the highest at 24 dph; although dead fish were usually small, disappearance due to cannibalism was not observed during the rearing period. The results indicate that aggressive behaviour in greater amberjack is induced by hunger and size diversity in the early juvenile stages (23 dph). Mass mortality during seedling production was mainly caused by injury and the death of small fish due to aggressive behaviour/cannibalism.
• 32) Effects of Seawater Elements on Growth, Survival Rate, and Whole-body Mineral Concentration of the Ocellate Puffer Takifugu rubripes larvae and Juveniles

  Tsuyoshi Nishida; Yasunori Ishibashi

  Aquaculture science 59 (1) 59 - 64 2011/01 [Refereed]
  
• Seed production technique in Pacific bluefin tuna Thunnus orientalis

  ISHIBASHI YASUNORI

  NSUGAF The Japanese Society of Fisheries Science 76 (5) 973 - 973 0021-5392 2010/09 

  Copyright (c) 2010 日本水産学会, rights: 本文データは学協会の許諾に基づきJ-STAGEから複製したものである, relation: isVersionOf: https://www.jstage.jst.go.jp/article/suisan/76/5/76_5_973/_article/-char/ja/
• 31) Spectral sensitivity of juvenile chub mackerel (Scomber japonicus) in visible and ultraviolet light

  Taro Matsumoto; Hiroshi Ihara; Yoshinari Ishida; Shinji Yamamoto; Osamu Murata; Yasunori Ishibashi

  FISH PHYSIOLOGY AND BIOCHEMISTRY SPRINGER 36 (1) 63 - 70 0920-1742 2010/03 [Refereed]
   

  Although chub mackerel (Scomber japonicus) is widely distributed all over the world, the relevance of its visual sensitivity to its ecology is not yet fully understood. We investigated spectral sensitivity in juvenile chub mackerel in the range of ultraviolet (UV) to visible light (369-652 nm) by electroretinogram (ERG) using light-emitting diodes (LEDs). Sensitivity peaked at a wavelength of approximately 482 nm in dark-adapted fish and 525 nm in light-adapted fish. A secondary sensitivity peak in the UV range at approximately 382 nm was found in both dark- and light-adapted fish. The UV sensitivity of chub mackerel may be attributable to UV transmissibility of the optical media and to the presence of a beta-band of visible light-sensitive visual pigments, and not to an alpha-band of UV visual pigments. This UV sensitivity may be useful for feeding or communication with other fishes.
• 30) Development of visual cells in the Pacific bluefin tuna Thunnus orientalis.

  Ryota Matsuura; Yoshifumi Sawada; Yasunori Ishibashi

  Fish physiology and biochemistry 36 391 - 402 2010 [Refereed]
  
• 29) Electroretinographic Analysis of Night Vision in Juvenile Pacific Bluefin Tuna (Thunnus orientalis)

  Taro Matsumoto; Hiroshi Ihara; Yoshinari Ishida; Tokihiko Okada; Michio Kurata; Yoshifumi Sawada; Yasunori Ishibashi

  BIOLOGICAL BULLETIN MARINE BIOLOGICAL LABORATORY 217 (2) 142 - 150 0006-3185 2009/10 [Refereed]
   

  We used electroretinogram recordings to investigate visual function in the dark-adapted eyes of the juvenile scombrid fishes Pacific bluefin tuna (Thunnus orientalis) and chub mackerel (Scomber japonicus) and the carangid fish striped jack (Pseudocaranx dentex). Despite the fast swimming speed of the Pacific bluefin tuna, analysis of flicker electroretinograms showed that visual temporal resolution in this species was inferior to that in chub mackerel. Peak wavelengths of spectral sensitivity in Pacific bluefin tuna and striped jack were 479 and 512 nm, respectively. The light sensitivity of Pacific bluefin tuna was comparable to that of chub mackerel but lower than that of striped jack. The Pacific bluefin tuna may not need high-level visual function under dim light conditions in natural habitat because it is a diurnal fish. However, this low temporal resolution and light sensitivity probably explain the mass deaths from contact or collisions with net walls in cultured Pacific bluefin tuna.
• 28) Artificial lighting prevents high night-time mortality of juvenile Pacific bluefin tuna, Thunnus orientalis, caused by poor scotopic vision

  Yasunori Ishibashi; Tomoki Honryo; Kazuki Saida; Akihiko Hagiwara; Shigeru Miyashita; Yoshifumi Sawada; Tokihiko Okada; Michio Kurata

  AQUACULTURE ELSEVIER SCIENCE BV 293 (3-4) 157 - 163 0044-8486 2009/08 [Refereed]
   

  High mortality following transfer of early juvenile Pacific bluefin tuna (PBT) aged around 30 days post hatching (about 5 cm total length) from hatchery tanks to sea net cage, particularly during the first few days, is a major problem inhibiting development of large-scale culture operations. Survival rate of the fish after stocking from land tank to a small sea cage was dropped to about 50% or less by the next morning, and became about 10% thereafter. In addition, the whole body cortisol levels increased significantly the next day. In a lab-based study using an infra-red camera, death was associated with fish touching and/or colliding with the net wall, then exhibiting abnormal swimming behavior, then becoming moribund during the night and dawn. In trials in sea net cages, overhead lights providing 200-3000 Ix intensity at the water surface, significantly improved survival to 73% up to 23 days post-transfer compared to 12% survival in un-lit control sea cage. Skin injuries were observed in most of the dead fish. The scotopic visual threshold of juvenile PBT was 11.1 log photons/cm(2)/s, at least 40 fold inferior to the threshold of 8.6-9.6 log photons/cm(2)/s exhibited by four marine teleosts, juvenile Pagrus major, Takifugu rubripes, Seriola dumerili, and Epinephelus septem-fasciatus. It was thought that the PBT had scotopic vision that was much less developed than that of other marine fish in the early juvenile stage, and they touched and/or came into collision with the net wall during the night and dawn. (C) 2009 Elsevier B.V. All rights reserved.
• 27) Spectral sensitivity in juvenile striped jack (Pseudocaranx dentex)

  Taro Matsumoto; Hiroshi Ihara; Yoshinari Ishida; Tokihiko Okada; Michio Kurata; Yoshifumi Sawada; Yasunori Ishibashi

  Aquaculture science 57 (2) 211 - 217 0371-4217 2009/06 [Refereed]
  
• 26) Three cone opsin genes and cone cell arrangement in retina of juvenile Pacific bluefin tuna Thunnus orientalis

  Taeko Miyazaki; Jun Kohbara; Kenji Takii; Yasunori Ishibashi; Hidemi Kumai

  FISHERIES SCIENCE SPRINGER TOKYO 74 (2) 314 - 321 0919-9268 2008/04 [Refereed]
   

  In bluefin tuna aquaculture, collision of juveniles with the tank or net walls is a major cause of high mortality. This problem may be related to color sensibility of the visual mechanisms of this species. As a first step in understanding of color vision of Pacific bluefin tuna Thunnus orientalis, we applied a molecular technique and histology to study cone cell distribution in the retina of juvenile fish. We isolated three cone opsin genes encoding one blue-sensitive (SWS2) and two green-sensitive (RH2) visual pigments. In situ hybridization revealed that SWS2 mRNA is localized in the single-cone photoreceptors. The localization of the two RH2 signals in distinct cone cells was not determined, probably because of the high homology between the two RH2 genes. Single-cone photoreceptors appeared frequently in the ventral-temporal retina in approximately 80-mm fish and in the temporal retina in approximately 230-mm fish. These cone distributions may define a visual field with best color contrast vision in front and above the fish with a short wavelength (blue) reflecting target (sensed by single cones), and may be enhanced against the longer wavelength (green) background when fish see a target below them (sensed by double cones).
• 25) Effects of hypoxia and hypercapnia on the embryonic development of striped jack, Pseudocaranx dentex

  Yoshifumi Sawada; Kazuhiro Higuchi; Yutaka Haga; Kazuhiro Ura; Yasunori Ishibashi; Michio Kurata; Hirofumi Miyatake; Shigekazu Katayama; Manabu Seoka

  Nippon Suisan Gakkaishi (Japanese Edition) 74 (2) 144 - 151 0021-5392 2008/03 [Refereed]
   

  The hypoxic condition, which sometimes occurs in the high-density layer of fish eggs during the procedures of egg collection and transportation in aquaculture, is thought to be accompanied by hypercapnia. This study investigated the effects of hypoxia and hypercapnia for the striped jack Pseudocaranx dentex embryos during somitogenesis. The somitic disturbances in newly hatched larvae were induced by the extreme hypoxia of exposure to 0 % dissolved oxygen (DO) concentration for 30 and 60 minutes. Extreme hypercapnia of 120 mg/L dissolved carbon dioxide (DCD) concentration for 90 or 120 minutes also induced the disturbances. The moderate hypoxic condition of exposure to 25% DO concentration for 60 and 90 minutes accompanied with hypercapnia of exposure to 120 mg/L DCD concentration induced somitic disturbances whereas this hypoxic condition alone did not induce them. The incidence rate of somitic disturbances was higher in the case of hypoxia of 25% DO and hypercapnia of 120 mg/L DCD than that in the case of normoxia of 100% DO and hypercapnia of 120 mg/L. These results indicate that hypoxia and hypercapnia during somitogenesis induce somitic disturbances as in other marine fishes and their coincidence promotes the induction in striped jack.
• 24) Ontogenic change of visual acuity in the ocellate puffer, Takifugu rubripes

  Takeshi Suzuki; Yasunori Ishibashi

  Aqaculture science 56 (1) 67 - 72 0371-4217 2008/03 [Refereed]
  
• 23) Evaluating developmental changes in schooling behavior in cultivated Pacific bluefin tuna Thunnus orientalis using a mathematical model

  H FUKUDA; S TORISAWA; K SUZUKI; Y ISHIBASHI; M KURATA; Y SAWADA; T TAKAGI; T YAMANE

  Contributions on the Theory of Fishing Gears and Related Marine Systems 5 297 - 305 2007/11 [Refereed]
  
• 22) Ontogenic changes in tolerance to hypoxia and energy metabolism of larval and juvenile Japanese flounder Paralichthys olivaceus

  Yasunori Ishibashi; Takeshi Kotaki; Yosuke Yamada; Hiromi Ohta

  JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY ELSEVIER SCIENCE BV 352 (1) 42 - 49 0022-0981 2007/11 [Refereed]
   

  Changes in tolerances to hypoxia and sodium azide, an indicator of cellular respiration, and activities of various energy metabolism-related chemical components were studied in Japanese flounder Paralichthys olivaceus during its early life stages from 3.5 to 20.5 mm in total length (TL). They showed flexion stage around 10.4 min TL. Lethal levels of hypoxia increased with growth from 3.5 to 8 min total TL, and the levels remained high in larvae, until 10.4 turn TL, decreased significantly thereafter. The 50% lethal concentration of sodium azide temporarily increased at 4.5 mm TL, diminished drastically between 4.5 and 10.4 nun TL, and then increased again in post-flexion larvae. Cytochrome c oxidase activity was highest in larvae around flexion, at 10.4 min TL, and subsequently decreased. In post-flexion larvae at 13.0 min TL, lactate dehydrogenase (LDH) and creatine kinase activities increased; LDH activity decreased at the juvenile stage. The adenosine triphosphate content and energy charge in fish were consistently higher in the larval stage than in the juvenile stage. These results indicated that, from just before flexion to the post-flexion stage, the energy metabolism of larvae is higher due to activated aerobic and subsequent anaerobic metabolism for metamorphosis; as a consequence, hypoxia tolerance in fish is the lowest during the increase of aerobic metabolism just before and around flexion. (c) 2007 Elsevier B.V. All rights reserved.
• 21) Changes in the retinal cone density distribution and the retinal resolution during growth of juvenile Pacific bluefin tuna Thunnus orientalis

  Shinsuke Torisawa; Tsutomu Takagi; Yasunori Ishibashi; Yoshifumi Sawada; Takeshi Yamane

  FISHERIES SCIENCE BLACKWELL PUBLISHING 73 (5) 1202 - 1204 0919-9268 2007/10 [Refereed]
  
• 20) Schooling behaviour and retinomotor response of juvenile Pacific bluefin tuna Thunnus otientalis under different light intensities

  S. Torisawa; T. Takagi; H. Fukuda; Y. Ishibashi; Y. Sawada; T. Okada; S. Miyashit; K. Suzuki; T. Yamane

  JOURNAL OF FISH BIOLOGY BLACKWELL PUBLISHING 71 (2) 411 - 420 0022-1112 2007/08 [Refereed]
   

  Schooling behaviour and histological retinal light adaptation in juvenile Pacific bluefin tuna Thunnus orientalis were examined under various light intensities to determine the effect of light intensity on behaviour. After monitoring the schooling behaviour of juveniles 35-36 and 45-46 days post hatching, schooling variables such as nearest neighbour distance and separation swimming index were measured under different light intensities. Furthermore, retinal indices of light adaptation were investigated histologically for each experimental light intensity. Under intensities >5 lx, schooling variables in the two juvenile growth stages were nearly constant, allowing schooling. In contrast, the schooling variables indicated that the fish gradually swam more widely and randomly with decreasing light intensities <5 lx. The retinal indices also showed a shift from light adaptation to dark adaptation at light levels <5 lx. From 5 to 0-01 lx, retinal adaptation and fish schooling behaviour changed with light intensity. These data suggest that the schooling behaviour of juvenile Pacific bluefin tuna is greatly affected by retinal adaptation. (c) 2007 The Authors Journal compilation (c) 2007 The Fisheries Society of the British Isles.
• Collision death in seedling production(Mini-Symposium,Early development and mass seedling production of bluefin tuna Thunnus orientalis-Present status and prospect-)

  ISHIBASHI YASUNORI; YASUNORI ISHIBASHI; Department of Fisheries School of Agriculture Kinki University

  NSUGAF The Japanese Society of Fisheries Science 72 (5) 949 - 950 0021-5392 2006/09
• 19) Effect of illumination intensity on schooling behaviour in the juvenile bluefin tuna

  Hiromu Fukuda; Shinsuke Torisawa; Yasunori Ishibashi; Michio Kurata; Yoshifumi Sawada; Katsuya Suzuki; Tsutomu Takagi

  Mem. Fac. Agr. Kinki Univ 39 89 - 93 2006
• 18) Early development and mass seedling production of bluefin tuna Thunnus orientalis—Present status and prospect—

  Yasunori Ishibashi

  Nippon Suisan Gakkaishi (Japanese Edition) 72 (5) 938 - 950 1349-998X 2006 [Invited]
  
• 17) Ontogeny of tolerance to hypoxia and oxygen consumption of larval and juvenile red sea bream, Pagrus major

  Y Ishibashi; K Inoue; H Nakatsukasa; Y Ishitani; S Miyashita; O Murata

  AQUACULTURE ELSEVIER SCIENCE BV 244 (1-4) 331 - 340 0044-8486 2005/02 [Refereed]
   

  Changes in tolerance to hypoxic stress and oxygen consumption were studied in the red sea bream, Pagrus major, from its early life stage until 42 days post-hatch. In the experiments, metamorphosis was observed mainly from days 15 to 30, and the morphological shift from larva to juvenile was completed at around 9.5 mm total length (TL). During the larval stage, lethally low dissolved oxygen (DO) levels and mass-specific metabolic rates increased with growth from 2.6 to 5 mm TL (P<0.01). Subsequently, the levels remained high and decreased until about 9.5 mm TL around the flexion stage and post flexion stage. Finally, beginning in the juvenile stage, lethal DO levels and mass-specific metabolic rates decreased as TL increased up to about 30 turn (P<0.01). The relationship between lethal DO levels and mass-specific metabolic rates was significantly linear (r=0.59,p<0.001, n=207) in fish larvae and juveniles. These results indicated that, around the stage of flexion and post flexion larvae in red sea bream, metabolic rates were highest during metamorphosis, and consequently hypoxia tolerance was lowest. It was presumed that the increasing metabolic rate during metamorphosis induced a decrease in the metabolic scope of activity and thereby induced the decrease of the tolerance to some environmental stressors in the background. (C) 2004 Elsevier B.V. All rights reserved.
• 16) Stress-induced apoptosis in larval embryos of Japanese flounder

  T Yabu; Y Ishibashi; M Yamashita

  FISHERIES SCIENCE JAPANESE SOC FISHERIES SCIENCE 69 (6) 1218 - 1223 0919-9268 2003/12 [Refereed]
   

  Stress-induced apoptosis was characterized in larval embryos of Japanese flounder using a caspase-3-like activity assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining of whole mounts. Severe stress conditions, such as heat shock by temperature shift from 16degreesC to 28degreesC or 29degreesC for 1 h, and ultraviolet (UV) irradiation at 20-100 MJ/cm(2), induced extensive apoptosis in embryos. Following the induction of stress, many TUNEL-positive apoptotic cells were found in the yolk sac, heart, and larval fin. Caspase-3-like activity against a fluorogenic substrate, acetyl-Asp-Glu-Val-Asp-alpha-4-methylcoumaryl-7-amide, increased in a dose-dependent manner during stress-induced apoptosis. In addition, C2-ceramide treatment (5-10 muM) induced a similar apoptosis pattern to stress treatment. These findings indicate that a pro-apoptotic pathway, which is caspase-3 mediated and acts through ceramide signaling, plays a major role in induction of apoptosis under stress conditions.
• 15) Ontogenic changes in various stress tolerances of larval and juvenile red sea bream Pagrus major

  Y Ishibashi; M Ozawa; H Hirata; H Kumai

  NIPPON SUISAN GAKKAISHI JAPANESE SOC FISHERIES SCIENCE 69 (1) 36 - 43 0021-5392 2003/01 [Refereed]
   

  Changes in tolerances to certain environmental stressors, e.g., low and high temperatures and salinity as well as high concentrations of ammonia, were studied in the red sea bream Pagrus major during its early life stage up to 42 days post-hatch. Relative tolerance was assessed by determining 24-h median lethal level. In laboratory experiments, metamorphosis was observed mainly from days 14-21, and the morphological shift from larva to juvenile was indicated at 9.5 mm total length at 28 days post-hatch. Among the stages tested, larva immediately after hatching showed maximum tolerance to all stressors. Tolerances to both low and high temperatures decreased up to 14 days post-hatch and increased thereafter. Hypo- and hyper-salinity tolerances also decreased up to the 14(th) day post-hatch and then showed a gentle increase. In regard to ammonia tolerance, the 50% lethal concentration diminished drastically between days 2 and 7, showed the lowest level on day 21, and subsequently rose. These results indicate that, in red sea bream under these experimental conditions, all tolerances to temperature, salinity, and ammonia stress temporarily fell during metamorphosis from days 14-21. It is considered that the depression of the scope for activity based on increasing oxygen consumption induced the decrease in various stress tolerances during metamorphosis. It is also suggested that the 50% lethal levels of various environmental stressors can be used as a stress test to evaluate the hardiness of a hatchery-produced red sea bream.
• 14) Stress response and energy metabolism in various tissues of Nile tilapia Oreochromis niloticus exposed to hypoxic conditions

  Y Ishibashi; H Ekawa; H Hirata; H Kumai

  FISHERIES SCIENCE JAPANESE SOC FISHERIES SCIENCE 68 (6) 1374 - 1383 0919-9268 2002/12 [Refereed]
   

  Nile tilapia were exposed to severe hypoxic conditions. The respiratory frequency (RF) of the fish reached a maximum level at approximately 10 mmHg PO2 and decreased gradually thereafter. The fish sank to the bottom and subsequently their respiration began to stop. The hypoxic conditions increased the hematocrit value. As RF decreased, plasma cortisol and glucose levels climbed drastically. Just before respiratory arrest, the ATP and the total adenylate (TA) concentrations were significantly depressed in the liver, kidney and ordinary muscle but not in the heart or gill. As RF decreased, ATIP and TA in both the liver and kidney decreased rapidly. Cytochrome oxidase activity increased significantly in the brain, heart, gill and ordinary muscle until respiratory frequency peaked. However, from the peak RF, this activity decreased in the liver and kidney. Lactic acid levels in both ordinary muscle and liver increased markedly when the fish sank. These results indicate that the stress response to hypoxic conditions is induced markedly with the decreased RF It is also suggested that the decreased energy status in the liver and kidney of tilapia occurs at the same stage as the metabolic depression in the whole body, without increases in the aerobic and anaerobic metabolisms.
• 13) Dietary pyridoxine requirement of juvenile Japanese parrot fish, Oplegnathus fasciatu

  Yasunori Ishibashi; Keitaro Kato; Shizunori Ikeda; Osamu Murata; Shigeru Miyashita; Hidemi Kumai

  Suisanzoshoku 水産増殖談話会 50 (3) 347 - 353 0371-4217 2002/09 [Refereed]
   

  平均体重5.4gのイシダイ稚魚を、ピリドキシン（PN)含量が1kg乾飼料当たり0.3から50.2mgの７飼料区の精製試験飼料でそれぞれ14週間飼育した。PN0.3mg区には飼育２週目から成長低下、神経過敏、遊泳異常、へい死魚の増加、正色素性小赤血球性貧血などの欠乏症状が確認された。PN1.4mg区でも飼育11週目から食欲が減退し、増重率が低下した。両区の半数の魚にPN 11.1mg/kg飼料を与えた結果、明らかな回復が認められた。試験終了時におけるPN 0.3および1.4mg区の血漿総タンパク質、グルコース、トリグリセリド含量および比肝重は他区に比べて低かった。PN 2.9mg以上の飼料区における魚の飼育成績、血液性状、血漿化学成分および比肝重に、顕著な差異はみられなかった。さらに魚体重100g当たりの肝臓GOTおよびGPT活性はPN 2.9mg以上で、肝臓PN含量はPN 11.1mg以上の飼料区でおよそ一定となった。Broken-line regression analysisにより、成長、肝臓GOTおよびGPT活性を指標としたイシダイ稚魚のPN要求量は、飼料乾重1kg当たり2.8~4.1mg、肝臓PN飽和量を指標とした場合には、11.4mgと判定された。
• 12) Stress response and energy status in various tissues of red sea bream, Pagrus major, subjected to hypoxic exposure

  Yasunori Ishibashi; Hachiro Hirata; Hidemi Kumai

  Suisanzoshoku Japanese Society for Aquaculture Science 50 (3) 315 - 323 0371-4217 2002/09 [Refereed]
   

  Young red sea bream, Pagrus major, were exposed to a gradual decrease in partial pressure of oxygen (Po₂) down to 20 mmHg for 5 h, and then sustained under 20 mmHg Po₂ for an additional 3 h at 19.9°C. Fish respiratory frequency slowly increased and attained a peak level at approximately 35 mmHg Po₂, and then gradually decreased. Fish sank to the tank bottom about an hour after Po₂ fell below 20 mmHg, and respiration began to arrest after another hour. Hematocrit value increased and mean cellular hemoglobin content (MCHC) decreased with increasing hypoxia load. Plasma cortisol and glucose levels were significantly augmented when respiratory frequency decreased. ATP, total adenylate (TA) concentration and energy charge (EC) in the hepatopancreas, in addition to TA in the kidney, were considerably diminished by hypoxia. However, this was not observed in the gill and brain of all fishes prior to respiratory arrest. In the hepatopancreas, levels of ATP, TA and EC notably declined as respiratory frequency decreased. These results show that the cortisol stress response to hypoxia is significantly induced with the decreased respiratory frequency. Decreased energy status in the hepatopancreas appears to be important and occurs with stress response as a metabolic depression in the whole body.
• 11) Response of enzyme activities and metabolite concentrations in various tissues of red sea bream, Pagrus major, subjected to hypoxic exposure

  Yasunori Ishibashi; Hiromichi Yonezawa; Shigeru Miyashita; Ketaro Kato; Osamu Murata; hachiro Hirata; Hidemi Kumai

  Suisanzoshoku 水産増殖談話会 50 (3) 325 - 331 0371-4217 2002/09 [Refereed]
   

  マダイ稚魚に、酸素分圧(Po2)を151から26mmHgまで２時間30分かけて低下し、26mmHg以下で約２時間放置するストレスを27.8℃で負荷した。呼吸数は徐々に増加し、38mmHg Po2前後で最大値を示してその後に低下した。26mmHg Po2以下では横転後に呼吸停止する魚が増えた。Ht値および血漿グルコース濃度はストレスの負荷に伴って増大し、MCHCは低下した。普通筋のCK、LDH活性および乳酸含量、並びに肝膵臓の乳酸含量は横転時に増加した。鰓のシトクロムcオキシターゼ(CCO)活性に顕著な変化はなかったが、脳のそれは低酸素負荷に伴って有意に増大した。一方、肝膵臓、腎臓および普通筋のCCO活性は、呼吸数最大時に激減したが、横転時以降には回復し、心臓のCCO活性も同時期に増加した。これよりマダイは、呼吸数最大時以降に低酸素負荷に対する代謝抑制を示すが、横転後には嫌気代謝の亢進とともに、その機能は解除されることが示唆された。
• 10) Diurnal changes of oxygen consumption in Devil Stinger Inimicus japonicus

  Hachiro Hirata; Masaya Niiro; Yasunori ishibashi; Osamu Murata; Hidemi Kumai

  Suisanzoshoku Japanese Society for Aquaculture Science 49 (4) 469 - 474 0371-4217 2001/12 [Refereed]
   

  In order to realize the biological management of the devil stinger Inimicus japonicus in culture, the diurnal changes of this species' oxygen consumption was recorded using a respiration monitor with a dissolved oxygen meter, YSI-57. Twenty-four fish were used. Their initial average body weight of fish was 76.3 ± 12.0 g. The fish were starved for 2 days before examination. They were then set one by one in a respiration chamber of 750 ml for 2 to 3 days. Water was pumped into the chamber by a peristaltic pump at a rate of 110 ml/min. The inlet water and outlet water of the chamber were pumped alternately to an oxygen electrode cell every 15 minutes, which process was controlled by a timer. The dissolved oxygen contents between inlet water and outlet water for the chamber were continuously recorded on a pen-recorder. The difference was determined manually from the recordings.
  The fish showed a bi-modal diurnal changes. The first peak of oxygen consumption, 178.8 ml/kg/h, was observed at twilight at 06:00. The second peak, 174.9 ml/kg/h, was recorded 2 to 3 hours after dusk, at 21:00. Lower rates of oxygen consumption, 154.8 ml/kg/h and 149.6 ml/kg/h, were found at 03:00 and 16:00, respectively. These results suggested that the oxygen generator should be operated at least twice a day, at around twilight and dust. The average rates of oxygen consumption in resting stage and active stage were 140.1 ± 64.8 ml/kg/h and 195.1 ± 89.1 ml/kg/h, respectively. The highly relationships between oxygen consumption in active and rest in fish were obtained in both twilight (r = 0.9618 with p < 0.001) and in dusk (r = 0.9625 with p < 0.001), respectively.
• 9) Ontogenetic change in oxygen consumption of Bluefin tuna, Thunnus thynnus

  Shigeru Miyashita; Nobuhiro Hattori; Yoshihumi Sawada; Yasunori Ishibashi; Hiromu Nakatsukasa; tokihiko Okada; Osamu Murata; Hidemi Kumai

  Suisanzoshoku 47 (2) 269 - 275 1999/06 [Refereed]
  
• 8) Qualitative Water-soluble Vitamin Requirements Of Tiger Puffer

  K KATO; Y ISHIBASHI; O MURATA; T NASU; S IKEDA; H KUMAI

  FISHERIES SCIENCE JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 60 (5) 589 - 596 0919-9268 1994/10 [Refereed]
   

  The qualitative requirements and deficiency signs of water-soluble vitamins were studied in tiger puffers Takifugu rubripes having mean body weight of 2.9 g. The qualitative requirements of tiger puffer for water-soluble vitamins indicate that the requirement of this species for vitamin B6 is lower than that of other fish species such as red sea bream, yellowtail, and Japanese parrot fish, while that for vitamins related to lipid metabolism, such as choline, nicotinic acid, pantothenic acid, biotin and inositol, is comparatively high.
• 7) Effect of dietary ascorbic acid supplementation on gonadal maturation in Japanese parrot fish.

  Yasunori Ishibashi; Keitaro Kato; Shizunori Ikeda; Osamu Murata; Toshiro Nasu; Hidemi Kumai

  Suisanzoshoku 水産増殖談話会 42 (2) 279 - 285 0371-4217 1994/06 [Refereed]
  
• 6) Comparison of growth and chemical composition between diploid and triploid in Japanese parrot fish.

  Osamu Murata; Keitaro Kato; Yasunori Ishibashi; Shigeru Miyashita; Toshiro Nasu; Shizunori Ikeda; Hidemi Kumai

  Aquaculture Science Japanese Society for Aquaculture Science 42 (3) 411 - 418 0371-4217 1994 [Refereed]
   

  The growth, maturation and chemical composition of normal diploid and triploid Japanese parrot fish, Oplegnathus fasciatus (TEMMINCK et SCHLEGEL) reared for 3 years 5 months were compared.
  Only slight differences between diploid and triploid fish in growth were observed throughout the experimental period.
  The gonado-somatic index of female triploid fish was consistently lower than that of female diploid fish. On the other hand, that of male triploid fish was higher than that of female triploid fish, and male triploid could be spawned by hand-stripping.
  In fish in the spawning period (3 and 4 years old), the % total edible portion (as percent of total body weight) in triploid fish was significantly higher than that of diploid fish.
  The lipid content of the viscera, liver and muscle in the diploid fish decreased between the pre-spawning and spawning periods, while in triploid fish, that of the viscera and abdominal muscle markedly increased during the same period. These results suggest that the accumulation of energy associated with sterility in triploid fish manifests as increase in the lipid content of the viscera and its limbus.
  In the spawning period, the crude protein and lipid contents of dorsal muscle in 4-year-old triploid fish were higher than those in diploid fish. On meat quality test, the taste and texture of female triploid fish were assessed as better than those of the female diploid.
• 5) EFFECTS OF DIETARY ASCORBIC-ACID ON TOLERANCE TO INTERMITTENT HYPOXIC STRESS IN JAPANESE PARROT FISH

  Y ISHIBASHI; K KATO; S IKEDA; O MURATA; T NASU; H KUMAI

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 58 (11) 2147 - 2152 0021-5392 1992/11 [Refereed]
   

  Control and stressed groups of juvenile Japanese parrot fish Oplegnathus fasciatus were fed diets (moist pellet) supplemented with 0, 75, and 300 mg of ascorbic acid (AsA) per 100 g. Fish in the stressed group were intermittently exposed to decreasing water oxygen levels every 3 or 4 days for 16 weeks. In the fish given a diet containing no AsA supplement, AsA-deficiency symptoms were manifested earlier, and to a greater extent, in the stressed fish than in the non-stressed ones. Similarly, the growth of stressed fish fed on a diet supplemented with 75 mg AsA per 100 g was inferior to the growth of the control group, and in the stressed fish there was a reduction in AsA levels in the plasma, kidneys, and gills. On the other hand, fish fed on a diet supplemented with 300 mg of AsA per 100 g were barely affected by the stressor. These results indicate that, in Japanese parrot fish under these experimental conditions, exposure to intermittent hypoxic stress not only induced AsA-deficiency disease early, but also increased the AsA requirement. It was also shown that high doses of AsA increased the ability of these fish to resist the stressor.
• 4) EFFECT OF DIETARY ASCORBIC-ACID ON THE TOLERANCE FOR LOW OXYGEN STRESS IN FISH

  Y ISHIBASHI; K KATO; S IKEDA; O MURATA; T NASU; H KUMAI

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 58 (8) 1555 - 1555 0021-5392 1992/08 [Refereed]
  
• 3) NUTRITIONAL-REQUIREMENTS OF THE JAPANESE PARROT FISH .3. OPTIMAL SUPPLEMENTARY ASCORBIC-ACID LEVEL IN THE JAPANESE PARROT FISH DIET

  Y ISHIBASHI; S IKEDA; O MURATA; T NASU; T HARADA

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 58 (2) 267 - 270 0021-5392 1992/02 [Refereed]
   

  Juvenile Japanese parrot fish Oplegnathus fasciatus were fed purified test diets supplemented with 0, 25, 50, 75, 100 and 300 mg of ascorbic acid (AsA) per 100 g of diet. In the no AsA supplemented diet group, growth declined and deficiency signs of deformity or abnormal swimming appeared from week 8. At week 16, the values of blood characteristics such as hemoglobin, hematocrit, glucose, total protein, alkaline phosphatase and calcium, and the hepatosomatic index of the fish fed diet containing no AsA supplement had significantly decreased than the other groups. On the other hand, normal growth and regular values of hematological parameters with no deformities were observed among the fish groups fed with diets with supplementary AsA levels of 25 mg per 100 g diet or greater. Moreover, the AsA contents of the brain, gill, kidney and liver of the fish were reached saturation level when the supplementary AsA level exceeded 50 mg per 100 g of diet. These results indicate that the sufficient supplementary AsA level to maintain normal growth and health of the Japanese parrot fish was estimated to be 25 mg of AsA per 100 g of diet under this experimental condition. It was also estimated that 50 mg of supplementary AsA per 100 g of diet is needed to saturate the tissue AsA levels.
• 2) NUTRITIONAL-REQUIREMENTS OF THE JAPANESE PARROT FISH .2. QUALITATIVE REQUIREMENTS OF THE JAPANESE PARROT FISH FOR WATER-SOLUBLE VITAMINS

  S IKEDA; Y ISHIBASHI; O MURATA; T NASU; T HARADA

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 54 (11) 2029 - 2035 0021-5392 1988/11 [Refereed]
  
• 1) NUTRITIONAL-REQUIREMENTS OF THE JAPANESE PARROT FISH .1. OPTIMUM LEVELS OF PROTEIN AND LIPID IN PURIFIED TEST DIET FOR THE JAPANESE PARROT FISH

  S IKEDA; Y ISHIBASHI; O MURATA; T NASU; T HARADA

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 54 (1) 151 - 154 0021-5392 1988/01 [Refereed]
  

Books etc

• 5)魚と植物を同時に育てるアクアポニックス，陸上でも魚を養殖，他，トコトンやさしい養殖の本

  石橋 泰典 近畿大学水産研究所編，日刊工業新聞 2019 68-89,96-99,102,156.
• Full-life cycled aquaculture of the Pacific bluefin tuna In: Kumai H, Miyashita S, Sakamoto W, Ono S (eds).

  Yasunori Ishibashi (Joint workFingerling production-II Flexion larvae to juveniles)Agriculture and forestry statistics publishing inc. 2012/03
• Aquaculture industry of Pacific bluefin tuna

  Yasunori Ishibashi (Joint workSeedling production technology)恒星社厚生閣 2011/03
• Kinki University project: Full cycled cultivation in Pacific bluefin tuna Thunnus orientalis

  Yasunori Ishibashi (Joint workIntermediate culture, Improvement of survival rate for juvenile)SEIZANDO-SHOTEN PUBLISHING CO.,LTD. 2010/03
• Japanese parrot fish (Oplegnathus fasciatus) and spotted parrot fish (Oplegnathus punctatus). In: Kumai H (eds)

  ISHIBASHI Yasunori Sobunsha 2000

Conference Activities & Talks

• 203)クロマグロ仔魚の初期飼育におけるナノバブルの有効性

  藤岡大嗣; 石橋泰典(近大院農)

  第11回 日本マイクロ・ナノバブル学会 学術総会 （東京）  2023/12
• 202)魚類のプロジェクター光による行動制御法の開発-Ⅶ アユ仔魚の摂餌率等に及ぼす光波長の影響

  青木政人; 石橋泰典(近大院農)

  令和5年度 日本水産増殖学会第21回大会（福井）  2023/12
• 201)魚類のプロジェクター光による行動制御法の開発-Ⅵ 数種養殖種苗の行動制御法の検討

  青木政人; 樫原由樹; 石橋泰典(近大院農)

  日本動物行動学会第42回大会 （京都）  2023/11
• 200)酸素ウルトラファインバブルがクエ仔魚の飼育成績に及ぼす影響

  藤岡大嗣; 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 199)クロマグロ仔魚の飼育成績に及ぼす水素ウルトラファインバブルの粒子密度の影響

  藤岡大嗣; 浅輪優樹; 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 198)魚類のプロジェクター光による行動制御法の開発-Ⅴ アユ仔稚魚の発育に伴う対光行動の変化

  青木政人; 樫原由樹; 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 197)魚類のプロジェクター光による行動制御法の開発-Ⅳ アユ仔稚魚の発育に伴う視物質オプシン遺伝子の発現様式

  青木政人; 樫原由樹; 竹中剛志; 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 196)ニホンウナギ仔魚の生残率等に及ぼす飼育水pHの影響

  永谷健一; 市原聡人(近大院農); 野村和晴; 石川卓; 金子信人(水産機構技術研); 田中秀樹(近大水研); 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 195)ニホンウナギ仔魚の生残率等に及ぼす各種飼育環境の影響

  永谷健一; 市原聡人(近大院農); 野村和晴; 石川卓; 金子信人; 福井洋平(水産機構技術研); 田中秀樹(近大水研); 石橋泰典(近大院農)

  日本水産学会 令和5年度日本水産学会秋季大会（仙台）  2023/09
• 194)魚類のプロジェクター光による行動制御法の開発-Ⅲ アユの生活史に伴う光誘導特性の変化

  青木政人; 樫原由樹; 竹中剛志; 石橋泰典(近大院農)

  第23回日本光生物学協会年会（京都）  2023/08
• 193)魚類のプロジェクター光による行動制御法の開発-Ⅱ 数種通し回遊魚の稚魚における対光行動の特性  [Not invited]

  青木政人; 樫原由樹; 石橋泰典(近大院農)

  第23回マリンバイオテクノロジー学会大会（石川）  2023/05
• 192)ニホンウナギ仔魚の生残率に及ぼす飼育水塩分の切り替えと循環飼育の影響  [Not invited]

  永谷健一; 市原聡人; 野村和晴; 石川卓; 金子信人; 田中秀樹; 石橋泰典

  日本水産学会 令和５年度日本水産学会春季大会（東京）（オンライン）  2023/03
• 191)ニホンウナギ仔魚の無給餌生残率に及ぼす飼育水塩分および水温の影響  [Not invited]

  永谷健一; 市原聡人; 野村和晴; 石川卓; 金子信人; 田中秀樹; 石橋泰典

  日本水産学会 令和５年度日本水産学会春季大会（東京）（オンライン）  2023/03
• 190)魚類のプロジェクター光による行動制御法の開発‐Ⅰ

  石橋泰典; 都築瞭斗; 青木政人; 上野未来; 松永康大(近大院農)

  日本水産学会 令和5年度日本水産学会春季大会（東京）  2023/03
• 189)クロマグロ仔魚の飼育成績に及ぼす酸素ウルトラファインバブルの粒子密度の影響  [Not invited]

  藤岡 大嗣; 石橋 泰典

  日本水産学会 令和4年度日本水産学会秋季大会（宮崎）  2022/09
• 188)クロマグロ仔魚の飼育成績に及ぼす数種ウルトラファインバブル水の影響  [Not invited]

  藤岡 大嗣; 石橋 泰典

  日本水産学会 令和4年度日本水産学会秋季大会（宮崎）  2022/09
• 187)数種気体を使ったウルトラファインバブルがクロマグロ仔魚の飼育成績に及ぼす影響  [Not invited]

  藤岡 大嗣 , 石橋 泰典

  第22回マリンバイオテクノロジー学会大会（オンライン）  2022/05
• 186)ニホンウナギ仔魚の無給餌生残率等に及ぼす飼育水塩分および元素組成の影響  [Not invited]

  永谷 健一; 市原 聡人; 鈴木 滉大; 高橋 裕太; 野村 和晴; 石川 卓; 金子 信人; 田中 秀樹; 石橋 泰典

  第22回マリンバイオテクノロジー学会大会（オンライン）  2022/05
• 185)クロマグロ仔魚の単色光照射が飼育成績に及ぼす影響  [Not invited]

  宇城 雄登; 石橋 泰典

  日本水産増殖学会第18回大会 令和元年度日本水産学会近畿支部例会（奈良）近畿大学  2019/11
• 184)クロマグロ仔魚の視物質オプシン遺伝子の発現と各種行動との関係  [Not invited]

  宇城 雄登; 石橋 泰典

  日本水産増殖学会第18回大会 令和元年度日本水産学会近畿支部例会（奈良）近畿大学  2019/11
• 183)カンパチ卵および仔魚の生残率等に及ぼす飼育水のポンプ循環流量および通気量の影響  [Not invited]

  石川 智将; 藤井 健太; 池田 大起; 石橋 泰典

  日本水産増殖学会第18回大会 令和元年度日本水産学会近畿支部例会（奈良）近畿大学  2019/11
• 182)二ホンウナギ稚魚と数種葉菜類とのアクアポニックスの検討 「★優秀ポスター賞・学生部門」  [Not invited]

  渡邉 れい子; 石橋 泰典

  日本水産増殖学会第18回大会 令和元年度日本水産学会近畿支部例会（奈良）近畿大学  2019/11
• 181)二ホンウナギ稚魚のアクアポニックスに関する研究  [Not invited]

  渡邉 れい子; 石橋 泰典

  近畿大学 第9回院生サミット （大阪）  2019/10
• 180)クロマグロ仔魚の光波長制御飼育に関する研究  [Not invited]

  宇城 雄登; 石橋 泰典

  近畿大学 第9回院生サミット （大阪）  2019/10
• 179) 二ホンウナギのアクアポニックスに使用する葉菜類の選択と適正生物量比の検討  [Not invited]

  渡邉 れい子; 石橋 泰典

  日本生物環境工学会 2019年千葉大会 (千葉）  2019/09
• 178) Opsin gene analysis and the effect of light wavelength on feeding and surface death in larval Pacific bluefin tuna(Thunnus orientalis)  [Not invited]

  USHIRO Yuto; ISHIBASHI Yasunori

  Marine Biotechnology Conference 2019 JAPAN  2019/09
• 177）Optimum biomass ratios in aquaponics of Japanese eel (Anguilla japonica) and Chinese water spinach(Ipomoea aquatica)  [Not invited]

  WATANABE Reiko; ISHIBASHI Yasunori

  Marine Biotechnology Conference 2019 JAPAN  2019/09
• 176) ウナギとクウシンサイのアクアポニックス  [Not invited]

  渡邉 れい子; 石橋 泰典

  日本水産学会 令和元年度日本水産学会秋季大会 （福井）  2019/09
• 175) クロマグロ仔魚の摂餌に及ぼす光波長の影響 「★優秀賞・学生部門」  [Not invited]

  宇城 雄登; 石橋 泰典

  日本水産学会 令和元年度日本水産学会秋季大会 （福井）  2019/09
• 174) 二ホンウナギとクウシンサイのアクアポニックスにおける生育環境条件の検討 「★学生優秀発表賞」  [Not invited]

  渡邉 れい子; 石橋 泰典

  近畿大学アグリ技術革新研究所報告会  2019/05
• Optimal water-tank shapes and water-current control systems for larval Longtooth grouper (Epinephelus bruneus)  [Not invited]

  HIroki Ikeda; Yasunori Ishibashi

  Korean Federation of Fisheries Science and technology Societies  2018/11
• 172) 低塩分海水が二ホンウナギ仔魚の無給餌生残指数等に及ぼす効果の生理的役割  [Not invited]

  高橋 祐太; 鈴木 滉大; 石橋 泰典; 野村 和晴; 須藤 竜介; 田中 秀樹

  日本水産学会 平成30年度日本水産学会秋季大会 （広島）  2018/09
• 171) ニホンウナギ仔魚の無給餌生残指数等に及ぼす各種海水，抗生物質および飼育容器の影響  [Not invited]

  鈴木 滉大; 高橋 祐太; 石橋 泰典; 野村 和晴; 須藤 竜介; 田中 秀樹

  日本水産学会 平成30年度日本水産学会秋季大会 （広島）  2018/09
• 170) 明暗および飼育水の水流発生法が，クエ仔魚の行動および飼育成績に及ぼす影響  [Not invited]

  池田 大起; 石橋 泰典

  日本水産学会 平成30年度日本水産学会秋季大会 （広島）  2018/09
• 169) 飼育密度がクエおよびクロマグロ仔魚の生残率，成長等に及ぼす影響  [Not invited]

  池田 大起; 武田 崇史; 石橋 泰典

  日本水産学会 平成30年度日本水産学会秋季大会 （広島）  2018/09
• 168) 通気量が難種苗生産種仔魚の生残率，成長等に及ぼす影響  [Not invited]

  池田 大起; 藤井 健太; 石橋 泰典

  日本水産増殖学会 平成30年度日本水産増殖学会大会 （神奈川）  2018/09
• 167) 水中ポンプの流量が難種苗生産種仔魚の生残率，成長等に及ぼす影響 「★学生ベストポスター賞」  [Not invited]

  池田 大起; 藤井 健太; 石橋 泰典

  日本水産増殖学会 平成30年度日本水産増殖学会大会 （神奈川）  2018/09
• Effects of seawater salinity on growth performance of juvenile thread-sail filefish Stephanolepis cirrhifer and application of recycled aquaculture system  [Not invited]

  Yasunori Ishibashi; Kei Suzaki; Misaki Sasagawa

  Asian-Pacific Aquaculture 2018  2018/04  Taipei, Taiwan
• 165) クエ仔魚の飼育成績に及ぼす水流発生法および高密度飼育の影響  [Not invited]

  池田 大起; 石橋 泰典

  日本水産学会 平成30年度日本水産学会春季大会 （東京）  2018/03
• 164) 小型水槽を用いたクエ仔魚の高密度飼育法の開発  [Not invited]

  池田 大起; 石橋 泰典

  日本水産学会 平成29年度日本水産学会近畿支部後期例会（京都）  2017/12
• 163) 紫外線AおよびBの照射がクロマグロの卵および仔魚の飼育成績等に及ぼす効果  [Not invited]

  辻 貴大; 石橋 泰典

  日本水産学会 平成29年度日本水産学会近畿支部後期例会（京都）  2017/12
• 162) 低塩分海水を用いたカワハギ稚魚とスジアオノリの閉鎖循環型複合養殖の検討  [Not invited]

  笹川 美沙季; 石橋 泰典

  日本水産増殖学会 平成29年度日本水産増殖学会大会 （愛媛）  2017/11
• 161) 飼育水のポンプ循環流量および通気量がカンパチ仔魚の飼育成績に及ぼす影響  [Not invited]

  藤井 健太; 石橋 泰典

  日本水産増殖学会 平成29年度日本水産増殖学会大会 （愛媛）  2017/11
• クエ仔魚の飼育成績に及ぼす通気量，油膜除去および海水塩分の影響  [Not invited]

  池田 大起; 石橋 泰典

  日本水産増殖学会 平成29年度日本水産増殖学会大会 （愛媛）  2017/11
• 159) 紫外線照射がクロマグロ，カンパチおよびクエの卵および仔魚の生残率等に及ぼす影響  [Not invited]

  辻 貴大; 石橋 泰典

  日本水産増殖学会 平成29年度日本水産増殖学会大会 （愛媛）  2017/11
• Effects of the light spectrum on the behavior, feeding, survival rate and growth of the larval longtooth grouper  [Not invited]

  Momoko Kobayashi; Yasunori Ishibashi

  Fisheries Science for Future Generations  2017/09
• The effects of water current control on the survival rate, feeding, and growth of larval longtooth grouper(Epinephelus Bruneus) 「the best student presentation」  [Not invited]

  HIroki Ikeda; Yasunori Ishibashi

  Fisheries Science for Future Generations  2017/09
• Effects of the tank shape, water current control and rearing density on the survival rate, feeding, and growth of pacific bluefin tuna, Thunnus orientails larvae  [Not invited]

  HIroki Ikeda; Takayuki Imagawa; Takashi Takeda; Yasunori Ishibashi

  Fisheries Science for Future Generations  2017/09
• The effects of rearing water salinity on the growth performance in juvenile ayu  [Not invited]

  Yoshikazu Sugiyama; Yasunori Ishibashi

  Fisheries Science for Future Generations  2017/09
• Influence of ultraviolet rays on the survival, feeding, and growth rate of the eggs and larvae of Pacific bluefin tuna (Thunnus orientalis)  [Not invited]

  Takahiro Tsuji; Yasunori Ishibashi

  Fisheries Science for Future Generations  2017/09
• 153) 外線照射がクロマグロ卵および仔魚の生残率等に及ぼす影響 「★優秀賞」  [Not invited]

  辻 貴大; 石橋 泰典

  近畿大学 第7回院生サミット （奈良）  2017/09
• 152) 光周期と暗期通気量がクロマグロ仔魚の生残・成長および鰾開腔に及ぼす影響  [Not invited]

  倉田 道雄; 田村 慶希; 本領 智記; 石橋 泰典; 澤田 好史

  日本水産学会 平成29年度日本水産学会春季大会 （東京）  2017/03
• 151) 飼育水の流動条件がクエ仔魚の成長，生残率等に及ぼす影響  [Not invited]

  池田 大起; 石橋 泰典

  日本水産学会 平成29年度日本水産学会春季大会 （東京）  2017/03
• 150) 環境低負荷・省エネ型種苗生産システムの構築  [Not invited]

  石橋 泰典

  平成28年度私立大学戦略的研究基盤形成支援事業プロジェクトシンポジウム（奈良）近畿大学  2017/02
• The cause of death of juvenile Pacific bluefin tuna （Thunnus orientalis) reared ? sea net cage.  [Not invited]

  Tomoki honryo; Tokihiko Okada; Masato Kawahara; Yoshifumi Sawada; Yasunori Ishibashi

  LACQUA16，Nov 28，2016，Lima, Peru．  2016/11
• Effects of photoenvironmental control on the feeding, survival rate and behaviour of the larval long-tooth grouper, epinephelus bruneus  [Not invited]

  Momoko Kobayashi; Yasunori Ishibash

  The Joint meeting of the 22nd International Congress of Zoology & the 87th Meeting of the Zoological Society of Japan  2016/11
• 147) 各種波長光がクエ仔魚の飼育成績等に及ぼす影響 「★学生ベストポスター賞」  [Not invited]

  小林 桃子; 石橋 泰典

  日本水産増殖学会 平成28年度日本水産増殖学会大会（三重）  2016/11
• 146) クロマグロの卵および仔魚の飼育成績等に及ぼす紫外線の影響  [Not invited]

  辻 貴大; 石橋 泰典

  日本水産増殖学会 平成28年度日本水産増殖学会大会（三重）  2016/11
• 145) クロマグロ仔魚の生残率，成長等に及ぼす水流発生法および高密度飼育の影響  [Not invited]

  池田 大起; 武田 崇史; 石橋 泰典

  日本水産増殖学会 平成28年度日本水産増殖学会大会（三重）  2016/11
• 144) クロマグロ種苗生産の各種技術的課題と対策，取り組み  [Not invited]

  石橋 泰典

  日本水産学会 第68回漁業懇話会講演会「クロマグロの持続的利用に向けた漁業技術研究」  2016/09
• 143) カワハギ稚魚の飼育成績，ストレス耐性等に及ぼす海水塩分の影響  [Not invited]

  須崎 慶; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 142) 持続的養殖プロ研マグロ‐31:クロマグロの中間育成期間における夜間給餌の影響  [Not invited]

  本領智記; 大水研; 山雄沙希; 岡田貴彦; 河原正人; 片山茂和; 倉田道雄; 澤田好史; 石橋泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 141) 持続的養殖プロ研マグロ‐30：クロマグロ稚魚の輸送および沖だし後の生残率等に及ぼす魚体サイズの影響と皮膚の発達  [Not invited]

  村山 昂; 本領 智記; 岡田 貴彦; 川原 正人; 片山 茂和; 澤田 好史; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 140) クロマグロ仔魚の視物質オプシン遺伝子の発現様式と遊泳行動，摂餌率，浮上死率等に及ぼす各種波長光の影響  [Not invited]

  長岡 生真; 三好 映実; 谷口 友希; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 139) クエ仔魚の生残率，摂餌，成長等に及ぼす水槽形状および水流発生法の影響  [Not invited]

  池田 大起; 山本 紗耶加; 北村 章博; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 138) 数種海産魚の卵および仔魚の生残率等に及ぼす海水塩濃度および元素組成の影響  [Not invited]

  上田 果奈; 太田 皓貴; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• 137) クエ仔魚の摂餌，浮上死率，遊泳行動等に及ぼす各種波長光の影響  [Not invited]

  小林 桃子; 石橋 泰典

  日本水産学会 平成28年度日本水産学会秋季大会 （奈良）  2016/09
• Development of yellowfin tuna (YFT) larviculture based on Pacific bluefin tuna (PBT) rearing technologies, and comparative studies of the vision characteristics of both species.  [Not invited]

  Yasunori Ishibashi; Gentoku Nakase; Tomoki Honryo; Yang-Su Kim; Taro Matsumoto; Angel Guillen; Michio Kurata; Alvaro Diaz; Yoshifumi Sawada; Amado Cano; Daniel Margulies; Vernon Scholey

  SATREPS-Programs on "Sustainable Aquatic Bioresources" that was held on December 19-20, 2015 in SATREPS  2015/12
• Development of yellowfin tuna larvae culture based on Pacific bluefin tuna rearing technologies, and comparative studies of the vision characteristics of both species  [Not invited]

  Yasunori Ishibashi; Gentoku Nakase; Tomoki Honryo; Yang-Su Kim; Taro Matsumoto; Angel Guillen; Michio Kurata; Alvaro Diaz; Yoshifumi Sawada; Amado Cano; Daniel Margulies; Vernon Scholey

  SATREPS International Symposium“Walk together towards the future; stably supplying high quality foods”  2015/11
• 134) クロマグロ仔魚の遊泳行動等に及ぼす各種光波長の影響  [Not invited]

  三好 映実; 谷口 友希; 石橋 泰典

  日本魚類学会 平成27年度日本魚類学会 第48回大会 （奈良）  2015/09
• 133) 照度がクロマグロ仔魚の摂餌, 成長, 鰾開腔および生残に及ぼす影響  [Not invited]

  田村 慶希; 倉田 道雄; 本領 智記; 石橋 泰典; 澤田 好史

  日本水産学会 平成27年度日本水産学会春季大会 （東京）  2015/03
• 132) 完全養殖クロマグロの量産化を目指して  [Not invited]

  石橋 泰典

  ビジネス・ロケーション・フォーラム 関西都市再生機構西日本支社，日本経済新聞大阪支社  2014/10
• 131) キハダのオプシン遺伝子  [Not invited]

  松本 太朗; 橋本 伸一郎; Daniel Margulies; Vernon P. Scholey (IATT; 石橋 泰典

  日本水産学会 平成26年度日本水産学会 秋季大会 （福岡）  2014/09
• 130) マグロ類の視覚特性比較  [Not invited]

  松本 太朗; 阿川 泰夫; 岡田 貴彦; 澤田 好史; 石橋 泰典

  日本水産学会 平成26年度日本水産学会 春季大会 (函館)  2014/03
• 129) マグロ類2種の環境適応能の初期発育とキハダ仔稚魚の飼育技術開発  [Not invited]

  石橋 泰典

  近畿大学第3回地球規模課題対応国際科学技術協力 シンポジウム「クロマグロとキハダの資源保護と中米キハダの完全養殖を目指して」  2014/03
• Ontogenic changes in tolerance to high- and low-temperature stressors of larval and juvenile Pacific bluefin tuna Thunnus orientalis.  [Not invited]

  石橋 泰典; 齋田 朋孝; Yazmin Villarreal; Angel Guillen(ARAP; 澤田 好史; 本領 智記

  Asian-Pacofoc Aquaculture 2013 Oct  2013/12
• Visual adaptation of longtooth grouper with growth.  [Not invited]

  Taro Matsumoto; Yasunori Ishibashi

  International conference on oceanography and sustainable marine production: A challenge of managing marine resources in a changing climate (ICOSMaP 2013)  2013/10
• 124) クロマグロ稚魚における鰾開腔不全と成長および前彎症との関連  [Not invited]

  倉田 道雄; 石橋 泰典; 本領 智記; 片山 茂和; 福田 漠生; 滝井 健二; 宮下 盛; 澤田 好史

  日本水産学会 平成25年度日本水産学会 秋季大会（三重）  2013/09
• 125) クロマグロ仔稚魚の発育に伴う低酸素耐性およびストレス応答の変化  [Not invited]

  石橋 泰典; 秋山 真理; 倉田 道雄; 岡田 貴彦

  日本水産学会 平成25年度日本水産学会 秋季大会（三重）  2013/09
• 126) クロマグロ仔稚魚の発育に伴う温度ストレス耐性およびHSP70 like遺伝子発現量の変化  [Not invited]

  石橋 泰典; 斉田 朋孝; Yazmin Villarreal; Angel Guillen; パナマ水産資源庁; 澤田 好史; 本領 智記

  日本水産学会 平成25年度日本水産学会 秋季大会（三重）  2013/09
• Development of feeding technology, formula diet, and parasite countermeasures for Pacific bluefin tuna, Thunnus orientalis  [Not invited]

  Ishibashi Y; Takii K; Murata O; Ishimaru K; Biswas A; Nakase G; Shirakashi S

  The Final GLOBAL COE International Symposium of Kinki University in Kushimoto,“Innovative Technology and Globalization of Tuna Aquaculture”  2012/11
• 121) クエのオプシン遺伝子の発現と視感度特性  [Not invited]

  松本 太朗; 石橋; 泰

  日本水産学会 平成24年度日本水産学会 秋季大会（下関）  2012/09
• 122) クロマグロ仔魚の生残率，腸管ワムシ数，成長等に及ぼす水槽形状および水流発生法の影響  [Not invited]

  今川 貴之; 倉田 道雄; 本領 智記; 阿川 泰夫; 澤田 好史; 石橋 泰典

  日本水産学会 平成24年度日本水産学会 秋季大会（下関）  2012/09
• Opsin gene cloning and electroretinogram analysis for thein longtooth grouper (Epinephelus bruneus)  [Not invited]

  Taro Matsumoto; Yasunori Ishibashi

  sia-Pacific Marine Biotechnology Conference (APMBC)  2012/07
• Determination of the optimal light wavelength for survival and growth performance and analysis of the opsin gene expression in the larvae of Pacific bluefin tuna (Thunus orientalis)  [Not invited]

  Shinichiro Hashimoto; Tomoki Honryo; Yasuo Agawa; Yoshifumi Sawada; Taro Matsumoto; Yasunori Ishibashi

  Asia-Pacific Marine Biotechnology Conference (APMBC)  2012/07
• Effects of photoenvironmental control on the feeding, growth, and survival rate of juvenile Pacific bluefin tuna (Thunnus orientalis)  [Not invited]

  Yasunori Ishibashi; Yoshiki Tsutsumi; Taro Matsumoto; Tomoki Honryo; Yoshifumi Sawada; Yasuo Agawa

  Asia-Pacific Marine Biotechnology Conference (APMBC)  2012/07
• Effects of the water current near the tank bottom on the feeding, growth, and survival rate of Pacific bluefin tuna (Thunnus orientails) larvae  [Not invited]

  Takayuki Imagawa; Michio Kurata; Tomoki Honryo; Yasuo Agawa; Yoshifumi Sawada; Yasunori Ishibashi

  Asia-Pacific Marine Biotechnology Conference (APMBC)  2012/07
• 116) クエ(Epinephelus bruneus)の視覚特性  [Not invited]

  松本 太朗; 石橋 泰典

  日本水産学会 平成24年度日本水産学会 春季大会  2012/03
• Cloning of the opsin gene in longtooth grouper (Epinephelus bruneus)  [Not invited]

  Taro Matsumoto; Yasunori Ishibashi

  SICB (The Society for Integrative and Comparative Biology) Annual Meeting 2012, Charleston, USA, 3-7  2012/01
• 114) クロマグロ 養殖グループの近況概略  [Not invited]

  石橋 泰典

  平成23年度グローバルCOEプログラム(東大阪)  2011/12
• Introducing of Early life history and rearing technology development team in Comparative Studies of the Reproductive Biology and Early Life History of Two Tuna Species for the Sustainable Use of These Resources. SATREPS Kick-Off Symposium, 17th Nov., 2011  [Not invited]

  Yasunori Ishibashi

  SATREPS Kick-Off Symposium, 17th Nov., 2011, Panama City.  2011/11
• 112) クエ(Epinephelus bruneus)のオプシン遺伝子  [Not invited]

  松本 太朗; 石橋 泰典

  日本水産学会 平成23年度日本水産学会 秋季大会 (長崎）  2011/09
• 110) カンパチ仔稚魚の飼育成績に及ぼす各種LED光源波長の影響  [Not invited]

  中嶌 匠; 石橋 泰典

  日本水産学会 平成23年度日本水産学会 秋季大会 (長崎）  2011/09
• 111) マダイ卵および仔稚魚の生残率等に及ぼす海水由来元素組成の影響  [Not invited]

  大土 誠; 石橋 泰典

  日本水産学会 平成23年度日本水産学会 秋季大会 (長崎）  2011/09
• 108) クロマグロThunnus orientalis仔魚の適正光波長  [Not invited]

  橋本 伸一郎; 本領 智記; 石橋 泰典

  日本動物学会 第82回大会 （旭川）  2011/09
• 109) クロマグロthunnus orientalis仔魚の沈降現象に及ぼす流動制御の影響  [Not invited]

  今川 貴之; 倉田 道雄; 石橋 泰典

  日本動物学会 第82回大会 （旭川）  2011/09
• 107) クロマグロ仔魚の行動の三次元解析  [Not invited]

  福田 漠生; 倉田 道雄; 中川 至純; 坂本 亘; 宮下 盛; 澤田 好史; 石橋 泰典; 高木 力

  日本水産学会 平成23年度日本水産学会 春季大会 (東京)  2011/03
• SEEDLING PRODUCTION OF THE PACIFIC BLUEFIN TUNA Thunnus orientalis AT KINKI UNIVERSITY  [Not invited]

  Yasunori Ishibashi

  Joint International Symposium on the 40th Anniversary of Pacific Bluefin Tuna Aquaculture 'Towards the Sustainable Aquaculture of Bluefin Tuna'  2010/10
• 104) 電照飼育における各種LED光源波長がクロマグロ稚魚の飼育成績に及ぼす影響  [Not invited]

  堤 吉輝; 松本 太朗; 本領 智記; 阿川 泰夫; 澤田 好史; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 秋季大会 （京都）  2010/09
• 105) スマ(Euthynnus affinis)のオプシン遺伝子  [Not invited]

  松本 太朗; 阿川 泰夫; 澤田 好史; 倉田 道雄; 岡田 貴彦; 石橋 泰典

  日本動物学会 第81回大会（東京）  2010/09
• Visual functions in full-cycle cultured juvenile Pacific bluefin tuna, Thunnus orientalis  [Not invited]

  Taro Matsumoto; Hiroshi Ihara; Yoshinari Ishida; Tokihiko Okada; Michio Kurata; Yoshifumi Sawada; Yasunori Ishibashi

  9th international congress on the biology of fish  2010/07
• Ontogenic changes in tolerance to hypoxia and energy metabolism of larval and juvenile red sea bream Pagrus major  [Not invited]

  Yasunori Ishibashi; Hiroe noguchi

  The American Fisheries Society-ELHS Santa Fe  2010/04
• 93) クロマグロ養殖業−技術開発と事業展開・展望−? クロマグロの人工種苗の量産化技術 5.種苗生産技術  [Not invited]

  石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会シンポジウム（東京）  2010/03
• 94) クロマグロ等の数種海産稚魚のハンドリング耐性とその発育に伴う変化  [Not invited]

  松山 景亮; 岡田 貴彦; 倉田 道雄; 澤田 好史; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 95) 電照飼育における各種LED光源波長がクロマグロ稚魚の飼育成績に及ぼす影響  [Not invited]

  堤 吉輝; 松本 太朗; 本領 智記; 阿川 泰夫; 澤田 好史; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 96) クロマグロ仔魚における沈降死の発生状況とその頻度に及ぼす水槽形状および水流の影響  [Not invited]

  石橋 泰典; 酒井 瑛介; 伊丹 翔太; 福田 漠生; 高木 力; 本領 智記

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 97) クロマグロ仔魚の浮上死発生率等に及ぼす水槽形状，水流および飼育密度の影響  [Not invited]

  石橋 泰典; 山中 一成; 小川 崇史; 西田 剛; 向井 良夫; 村田 修

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 98) クロマグロ仔魚の摂餌量，成長，生残率等に及ぼす各種LED光源波長の影響  [Not invited]

  中嶌 匠; 堤 吉輝; 倉田 道雄; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 99) クロマグロ稚魚の明順応時における視覚特性  [Not invited]

  松本 太朗; 倉田 道雄; 岡田 貴彦; 澤田 好史; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 100) 船舶輸送における活魚槽の壁面模様および照度変化がクロマグロ幼魚の生残率等に及ぼす影響  [Not invited]

  岡田 貴彦; 中谷 正宏; 向井 良夫; 澤田 好史; 宮下 盛; 熊井 英水; 石橋 泰典

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 101) クロマグロ人工種苗生産の産業化  [Not invited]

  岡田 貴彦; 倉田 道雄; 高岡 治; 向井 良夫; 田村 利博; 石橋 泰典; 半澤 淳也; 若杉 金市郎; 宮下 盛; 澤田 好史; 滝井 健二; 家戸 敬太郎; 村田 修; 熊井 英水

  日本水産学会 平成22年度日本水産学会 春季大会(東京）  2010/03
• 92) クロマグロ等の時間分解能と視感度  [Not invited]

  松本 太朗; 伊原 大志; 石田 義成(O; 澤田 好史; 岡田 貴彦; 山本 眞司; 村田 修; 石橋 泰典

  日本動物学会 第80回大会 (静岡)  2009/09
• 91) クロマグロ人工種苗の量産化-2−中間育成− 「クロマグロ養殖業の現状と展望」  [Not invited]

  石橋 泰典

  近畿大学平成21年度グローバルCOEプログラム 第1回シンポジウム（東京）  2009/07
• 90) 幼魚期の成長に伴うクロマグロ魚群の個体間相互作用の変化  [Not invited]

  福田 漠生; 高木 力; 石橋 泰典; 倉田 道雄; 澤田 好史

  日本水産学会 平成21年度日本水産学会 春季大会（東京）  2009/03
• Effects of artificial seawater composition on growth, survival rate and whole body elementary composition in larval and juvenile ocellate puffer Takifugu rubripes, red sea bream Pagrus major and purplish amberjack Seliola dumerili  [Not invited]

  西田 剛; 高橋 範行; 芝 広和; 中務 寛; 村田 修; 石橋 泰典

  5th World Fisheries Congress  2008/10
• 88) 魚類の網膜電図とフィッティング関数  [Not invited]

  松本 太朗; 伊原 大志; 山本 眞司; 村田 修; 石橋 泰典

  日本動物学会 第79回大会 （福岡）  2008/09
• 82) クロマグロ幼魚の推進エネルギーと移動コスト視覚特性が群行動に与える影響  [Not invited]

  高木 力; 石橋 泰典; 山根 猛; 福田 漠生; 鈴木 勝也; 山根 猛; 澤田 好史; 岡田 貴彦

  日本水産学会 平成20年度日本水産学会 春季大会（静岡）  2008/03
• 83) クロマグロ幼魚の視覚特性が群行動に与える影響1  [Not invited]

  鳥澤 眞介; 福田 漠生; 高木 力; 石橋 泰典; 澤田 好史; 山根 猛

  日本水産学会 平成20年度日本水産学会 春季大会（静岡）  2008/03
• 84) 高速度カメラを用いたクロマグロ幼魚の群行動解析  [Not invited]

  福田 漠生; 鳥澤 眞介; 鈴木 勝也; 石橋 泰典; 倉田 道雄; 澤田 好史; 高木 力

  日本水産学会 平成20年度日本水産学会 春季大会（静岡）  2008/03
• 85) クロマグロ，マサバおよびシマアジ稚魚の網膜電図と視覚特性  [Not invited]

  松本 太朗; 伊原 大志; 石田 義成(O; 澤田 好史; 岡田 貴彦; 山本 眞司; 村田 修; 石橋 泰典

  日本水産学会 平成20年度日本水産学会 春季大会（静岡）  2008/03
• 86) クロマグロおよびマサバ仔稚魚における網膜組織構造の発育変化  [Not invited]

  鈴木 誉士; 村松 亮兵; 倉田 道雄; 山本 眞司; 村田 修; 石橋 泰典

  日本水産学会 平成20年度日本水産学会 春季大会（静岡）  2008/03
• Visual sensitivity in juvenile Pacific bluefin tuna, chub mackerel, and striped jack  [Not invited]

  松本 太朗; 井原 大志; 石田 義成; 倉田 道雄; 岡田 貴彦; 澤田 好史; 石橋 泰典

  Seminor on new directions in marine science and aquaculture  2008/03
• Molecular cloning of opsin genes and their expression during larval and juvenile development in Chub mackerel Scomber japonicus  [Not invited]

  鈴木 誉士; 山本 眞司; 村田 修; 石橋 泰典

  Canadian Conference for Fisheries research, meeting, Halifax  2008/01
• Visual sensitivity in juvenile Pacific bluefin tuna, Thunnus orientalis  [Not invited]

  松本 太朗; 井原 大志; 石田 義成; 倉田 道雄; 岡田 貴彦; 澤田 好史; 石橋 泰典

  Canadian Conference for Fisheries research, meeting, Halifax  2008/01
• 79) クロマグロの種苗生産−大量死の原因と防止法. 「クロマグロ等の養殖産業の発展を期して」  [Not invited]

  石橋 泰典

  近畿大学平成19年度21世紀COEプログラム 最終シンポジウム （奈良）  2007/12
• Changes in retinal structure during the development of the puffer fish Takifugu Rubripes  [Not invited]

  T. Suzuki; Y. Ishibashi

  Aquaculture Europe 2007 Annuanl meeting  2007/10
• 76) マサバのオプシン遺伝子のクローニングと仔稚魚期における各オプシン遺伝子の発現時期  [Not invited]

  鈴木 誉士; 山本 眞司; 村田 修; 石橋 泰典

  日本水産学会 平成19年度日本水産学会 秋季大会（北海道）  2007/09
• 77) トラフグ，マダイおよびカンパチ仔稚魚の生残率等に及ぼす海水塩類組成の影響  [Not invited]

  西田 剛; 高橋 範行; 芝 広和; 中務 寛; 村田 修; 石橋 泰典

  日本水産学会 平成19年度日本水産学会 秋季大会（北海道）  2007/09
• 73) 網膜組織構造と視物質組成からみたトラフグ仔稚魚期の視覚特性とその発育変化  [Not invited]

  鈴木 誉士; 石橋 泰典

  日本水産増殖学会 平成19年度日本水産増殖学会大会（東京）  2007/07
• 74) クロマグロ稚魚の攻撃行動等に及ぼす各種飼育条件の影響  [Not invited]

  三木 孝久; 倉田 道雄; 澤田 好史; 石橋 泰典

  日本水産増殖学会 平成19年度日本水産増殖学会大会（東京）  2007/07
• 75) トラフグ仔稚魚の生残率等に及ぼす海水塩類組成の影響  [Not invited]

  西田 剛; 芝 広和; 中務 寛; 村田 修; 石橋 泰典

  日本水産増殖学会 平成19年度日本水産増殖学会大会（東京）  2007/07
• 66) クロマグロ幼魚の照度変化に対する応答? -行動と網膜運動反応  [Not invited]

  鳥澤 眞介; 福田 漠生; 高木 力; 石橋 泰典; 澤田 好史; 山根 猛

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 67) クロマグロ幼魚の照度変化に対する応答? -時間経過に伴う群行動特性の変化  [Not invited]

  福田 漠生; 鳥澤 眞介; 鈴木 勝也; 石橋 泰典; 倉田 道雄; 澤田 好史; 高木 力

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 68) クロマグロ稚魚の網膜における巨大神経節細胞の形態と分布  [Not invited]

  宮崎 多恵子; 神原 淳; 滝井 健二; 石橋 泰典; 熊井 英水

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 69) トラフグ仔稚魚における網膜組織構造の発育変化  [Not invited]

  鈴木 誉士; 石橋 泰典

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 70) カンパチ卵および仔稚魚の成長，生残率，全魚体ミネラル含量等に及ぼす海水塩類組成の影響  [Not invited]

  西田 剛; 高橋 範行; 芝 広和; 中務 寛; 村田 修; 石橋 泰典

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 71) カンパチ稚魚の攻撃行動等に及ぼす各種飼育条件および日令の影響  [Not invited]

  三木 孝久; 中務 寛; 高橋 範行; 横山 達雄; 村田 修; 石橋 泰典

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 72) クロマグロ稚魚の薄明視と沖出し直後の大量へい死  [Not invited]

  石橋 泰典; 斎田 一樹; 萩原 明彦; 岡田 貴彦

  日本水産学会 平成19年度日本水産学会春季大会（東京）  2007/03
• 61) クロマグロ仔稚魚の発育に伴うハンドリング耐性およびコルチゾル-ストレス反応の変化  [Not invited]

  村瀬 未来; 倉田 道雄; 澤田 好史; 石橋 泰典

  日本水産増殖学会 平成18年度日本水産増殖学会大会（山口）  2006/10
• 62) クロマグロ稚魚の電照飼育が沖出し後の生残率等に及ぼす影響  [Not invited]

  本領 智記; 岡田 貴彦; 倉田 道雄; 田村 利博; 石橋 泰典

  日本水産増殖学会 平成18年度日本水産増殖学会大会（山口）  2006/10
• 63) カンパチ卵および仔魚の生残率等に及ぼす海水塩類組成の影響  [Not invited]

  西田 剛; 高橋 範行; 芝 広和; 中務 寛; 村田 修; 石橋 泰典

  日本水産増殖学会 平成18年度日本水産増殖学会大会（山口）  2006/10
• 64) マダイ卵および仔魚の飼育成績，ストレス耐性，体成分組成に及ぼす海水塩類組成の影響  [Not invited]

  石橋 泰典; 石野 元美

  日本水産増殖学会 平成18年度日本水産増殖学会大会（山口）  2006/10
• 65) クエ稚魚の共食い行動に及ぼす各種飼育条件の影響  [Not invited]

  三木 孝久; 中務 寛; 横山 達雄; 村田 修; 石橋 泰典

  日本水産増殖学会 平成18年度日本水産増殖学会大会（山口）  2006/10
• 60) 種苗生産における衝突死 「クロマグロの初期発育と種苗生産，現状と展望」  [Not invited]

  石橋 泰典

  日本水産学会 平成18年度日本水産学会春季大会 ミニシンポジウム(高知）  2006/03
• 56) 行動モデルを用いたクロマグロ幼魚の群行動評価  [Not invited]

  福田 漠生; 鳥澤 眞介; 内山 哲朗; 石橋 泰典; 倉田 道雄; 澤田 好史; 高木 力

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 57) クロマグロ幼魚の視覚特性が群行動に与える影響  [Not invited]

  鳥澤眞介; 福田漠生; 高木 力; 石橋泰典; 澤田好史; 山根 猛

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 58) クロマグロ稚魚の色覚  [Not invited]

  宮崎 多恵子; 神原 淳; 滝井 健二; 石橋 泰典; 熊井 英水

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 59) クロマグロの網膜構造  [Not invited]

  松浦 良太; 澤田 好史; 石橋 泰典

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 53) クロマグロ幼魚の大量へい死とその防止法-3 衝突死の発生頻度等に及ぼす水槽およびイケス壁面模様の影響  [Not invited]

  石橋 泰典; 泉 達也; 岡田 貴彦; 倉田 道雄

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 54) クロマグロ幼魚の大量へい死とその防止法-2 衝突死の発生頻度等に及ぼす電照飼育の影響  [Not invited]

  本領 智記; 倉田 道雄; 岡田 貴彦; 澤田 好史; 石橋 泰典

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 55) クロマグロ幼魚の大量へい死とその防止法-1 ハンドリングに対するストレス反応および耐性の発育変化  [Not invited]

  村瀬 未来; 岡田 貴彦; 倉田 道雄; 石橋 泰典

  日本水産学会 平成18年度日本水産学会春季大会（高知）  2006/03
• 52) 衝突死の発生防止法 「クロマグロの持続的養殖生産，肉質・安全性そして流通」  [Not invited]

  石橋 泰典

  近畿大学 平成17年度21世紀COEプログラム成果報告シンポジウム （東大阪）  2006/02
• 48) クロマグロの網膜は色素上皮細胞によって区切られる  [Not invited]

  松浦 良太; 澤田 好史; 石橋 泰典

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/09
• 49) クロマグロの強制遊泳に対するストレス反応と普通筋重量比の発育変化  [Not invited]

  本好 知央; 岡田 貴彦; 澤田 好史; 石橋 泰典

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/09
• 50) クロマグロ稚魚の生残率およびストレス反応に及ぼすハンドリングの影響  [Not invited]

  村瀬 未来; 倉田 道雄; 澤田 好史; 石橋 泰典

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/09
• 51) クロマグロの衝突死発生原因と防止法-2 生残率，全魚体コルチゾル含量等に及ぼす明暗周期の影響  [Not invited]

  本領 智記; 倉田 道雄; 那須 敏朗; 宮下 盛; 石橋 泰典

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/09
• 46) クロマグロ稚魚の輸送と取扱いに伴うストレス反応  [Not invited]

  石橋 泰典; 中山 裕康; 岡田 貴彦; 宮下 盛

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/08
• 47) クロマグロの衝突死発生原因と防止法-1 視覚刺激に対する驚愕行動およびストレス反応  [Not invited]

  石橋 泰典; 小出 創太郎; 岡田 貴彦; 小田 誠二; 宮下 盛

  日本水産増殖学会 平成17年度日本水産増殖学会大会（福井）  2005/08
• 44) クロマグロ稚魚の驚愕反応およびストレス反応に及ぼす水槽色の影響  [Not invited]

  石橋 泰典; 小出 創太郎; 岡田 貴彦; 倉田 道雄; 澤田 好史; 小田 誠二; 宮下 盛

  マリンバイオテクノロジー学会 平成17年度大会（熊本）  2005/05
• 45) クロマグロ仔魚における網様体ニューロン群の形態  [Not invited]

  松浦 良太; 石橋 泰典

  マリンバイオテクノロジー学会 平成17年度大会（熊本）  2005/05
• 41) クロマグロ幼魚の群行動と視覚?−成長にともなう視覚特性の変化  [Not invited]

  鳥澤 眞介; 高木 力; 石橋 泰典; 澤田 好史; 山根 猛

  日本水産学会 平成17年度日本水産学会春季大会（東京）  2005/04
• 42) クロマグロにおける視細胞の発達過程  [Not invited]

  松浦 良太; 大畑 尚輝; 澤田 好史; 石橋 泰典

  日本水産学会 平成17年度日本水産学会春季大会（東京）  2005/04
• 43) クロマグロにおける衝突多発期前後のエネルギー代謝と強制遊泳の影響  [Not invited]

  本好 知央; 倉田 道雄; 岡田 貴彦; 石橋 泰典

  日本水産学会 平成17年度日本水産学会春季大会（東京）  2005/04
• 40) クロマグロの衝突死とその発生予防法について 「マグロ養殖産業の未来に向けて」  [Not invited]

  石橋 泰典

  近畿大学平成16年度21世紀COEプログラム 成果報告シンポジウム（東大阪）  2005/03
• 39) Ontogeny of hypoxic tolerance and energy metabolism of larvae and juvenile in Japanese flounder Paralichthys olivaceus.  [Not invited]

  Yasunori Ishibashi; Takeshi Kotaki; Michitaka Kataoka; Yoichi Yamada

  Europian Aquaculture Society 2004 Biotechnologies for Quality. Barcelona, Spain.  2004/10
• 38) 太平洋クロマグロ Thunnus orientalis 若魚の低酸素適応方法  [Not invited]

  森川 真理; 岡田 貴彦; 澤田 好史; 太田 博巳; 石橋 泰典

  日本魚類学会 2004年度日本魚類学会年会（沖縄）  2004/09
• 37) クロマグロの環境ストレスと衝突死 「クロマグロ研究の最前線」  [Not invited]

  石橋 泰典

  近畿大学 平成16年度21世紀COEプログラム シンポジウム  2004/07
• Ontogenic changes of various stress tolerances and stress response in larval and juvenile Japanese flounder Paralichthys olivaceus: role of cortisol to environmental stressors.  [Not invited]

  Yasunori Ishibashi; Takeshi Kotaki; Kazuaki Ryu

  Nordic Society of Fish Immunology The Sixth International Symposium of Fish Immunology. Turku, Finland  2004/05
• 34) クロマグロにおける低酸素耐性およびストレス反応の発育変化  [Not invited]

  森川 真理; 石橋 泰典; 倉田 道雄; 宮下 盛; 熊井 英水; 太田 博巳

  日本水産学会 平成16年度日本水産学会春季大会（鹿児島）  2004/04
• 35) 強制遊泳に伴うクロマグロのエネルギー代謝と衝突多発期前後の変化  [Not invited]

  石橋 泰典; 本好 知央; 岡田 貴彦; 澤田 好史; 熊井 英水

  日本水産学会 平成16年度日本水産学会春季大会（鹿児島）  2004/04
• 30) 低酸素負荷に対するトラフグのストレス反応および各種組織のエネルギー状態  [Not invited]

  工島 隆矢; 石橋 泰典; 太田 博巳; 熊井 英水

  日本水産増殖学会 平成15 年度日本水産増殖学会大会（北海道）  2003/10
• 31) 低酸素負荷に対するクロマグロのストレス反応と代謝への影響  [Not invited]

  森川 真理; 石橋 泰典; 倉田 道雄; 澤田 好史; 熊井 英水; 太田 博巳

  日本水産増殖学会 平成15 年度日本水産増殖学会大会（北海道）  2003/10
• 32) 撹乱負荷に対するクロマグロのストレス反応と代謝への影響  [Not invited]

  石橋 泰典; 本好 知央; 岡田 貴彦; 田村 利博; 宮下 盛; 熊井 英水

  日本水産増殖学会 平成15 年度日本水産増殖学会大会（北海道）  2003/10
• 33) リュウキュウアユ精子の凍結保存  [Not invited]

  津高 窓加; 竹村 明洋; 瀬良 垣貢; 石橋 泰典; 太田 博巳

  日本魚類学会 2003 年度日本魚類学会年会（京都）  2003/10
• 27) アユ精子の凍結保存  [Not invited]

  津高 窓加; 石橋 泰典; 太田 博巳; 熊井 英水

  日本水産学会 平成15年度日本水産学会春季大会（東京）  2003/04
• 28) トラフグ仔稚魚の低酸素負荷に対するストレス応答の発育変化  [Not invited]

  工島 隆矢; 石橋 泰典; 太田 博巳; 熊井 英水

  日本水産学会 平成15年度日本水産学会春季大会（東京）  2003/04
• 29) マダイ稚魚の各種環境負荷に対するストレス蛋白質転写因子等の発現様式  [Not invited]

  牧田 裕介; 石橋 泰典; 太田 博巳; 熊井 英水

  日本水産学会 平成15年度日本水産学会春季大会（東京）  2003/04
• 25) ヒラメ仔稚魚における環境ストレス適応能の発達 2 エネルギー代謝の発育変化とストレス耐性等に及ぼすコルチゾルの影響  [Not invited]

  石橋 泰典; 小滝 武志; 片岡 通孝; 熊井 英水; 平田 八郎

  日本水産学会 平成14年度日本水産学会春季大会（奈良）  2002/04
• 26) ヒラメ仔稚魚における環境ストレス適応能の発達 1 ストレス耐性の発育変化とストレス反応の発達  [Not invited]

  石橋 泰典; 小滝 武志; 劉 和明; 山田 洋介; 熊井 英水; 平田 八郎

  日本水産学会 平成14年度日本水産学会春季大会（奈良）  2002/04
• 24) 魚類の初期発育過程におけるアポトーシスの解析-? ヒラメ胚のストレス誘導性アポトーシス  [Not invited]

  藪 健史; 山下倫明; 石橋 泰典

  日本水産学会 平成13年度日本水産学会春季大会（藤沢）  2001/04
• 23) マダイ仔稚魚の発育に伴う呼吸阻害耐性、エネルギー充足率、各種酵素活性の変動  [Not invited]

  石橋 泰典; 熊井 英水; 平田 八郎

  日本水産学会 平成12年度日本水産学会秋季大会（福井）  2000/04
• 22) 低酸素ストレスの負荷に対するマダイの組織エネルギー充足率, 血液化学成分量等の変動  [Not invited]

  石橋 泰典; 宮下 盛; 家戸 敬太郎; 村田 修; 熊井 英水

  日本水産学会 平成9年度日本水産学会秋季大会（広島）  1997/04
• 21) オニオコゼとアナアオサ変異種の調和養殖に関する研究−? オニオコゼの酸素消費量の日周変化について  [Not invited]

  新納 正也; 平田 八郎; 石橋 泰典; 村田 修; 高橋 範行; 植田 嘉造

  日本水産学会 平成8年度日本水産学会 近畿支部例会 （奈良）  1996/10
• 19) マダイ仔稚魚の発育に伴う環境ストレス耐性の変動  [Not invited]

  石橋 泰典; 中務 寛; 宮下 盛; 村田 修; 熊井 英水; 平田 八郎

  日本水産学会 平成8年度日本水産学会春季大会（神奈川）  1996/04
• 20) アナアオサ変異種の生長とpH, DO, 光量  [Not invited]

  新納 正也; 平田 八郎; 秋山 真一; 石橋 泰典; 村田 修; 高橋 範行

  日本水産学会 平成8年度日本水産学会春季大会（神奈川）  1996/04
• 17) オニオコゼとアナアオサ変異種の調和養殖に関する研究−? 水槽型調和養殖システムにおける水質の動態  [Not invited]

  新納 正也; 平田 八郎; 石橋 泰典; 村田 修; 高橋 範行; 植田 嘉造

  日本水産学会 平成7年度日本水産学会秋季大会（京都）  1995/09
• 18) オニオコゼとアナアオサ変異種の調和養殖に関する研究−? 水槽型調和養殖システムの試作  [Not invited]

  平田 八郎; 新納 正也; 石橋 泰典; 村田 修; 高橋 範行; 那須 敏朗

  日本水産学会 平成7年度日本水産学会秋季大会（京都）  1995/09
• 16) マダイ仔稚魚の成長に伴う低酸素ストレス耐性の変動  [Not invited]

  石橋 泰典; 村田 修; 宮下 盛; 石谷 大; 熊井 英水; 平田 八郎

  日本水産学会 平成7年度日本水産学会春季大会（東京）  1995/04
• 15) カンパチおよびヒラマサ稚魚用飼料の至適タンパク質および脂質含量  [Not invited]

  家戸 敬太郎; 村田 修; 宮下 盛; 那須 敏朗; 池田 静徳; 石橋 泰典; 熊井 英水

  日本水産学会 平成6年度日本水産学会春季大会（東京）  1994/04
• 14) イシダイの２倍体と３倍体との成長および体成分組成の比較  [Not invited]

  村田 修; 家戸 敬太郎; 石橋 泰典; 池田 静徳; 宮下 盛; 那須 敏朗; 熊井 英水

  日本水産学会 平成5年度日本水産学会春季大会（東京）  1993/10
• 13) トラフグにおける水溶性ビタミンの要求性  [Not invited]

  家戸 敬太郎; 石橋 泰典; 池田 静徳; 村田 修; 那須 敏朗; 熊井 英水

  日本水産学会 平成5年度日本水産学会春季大会（東京）  1993/04
• 11) イシダイ飼料の至適ビタミンＢ6添加量  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗; 熊井 英水

  日本水産学会 平成4年度日本水産学会秋季大会 （下関）  1992/10
• 12) イシダイの低酸素ストレス耐性に及ぼす飼料アスコルビン酸投与の効果  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗; 熊井 英水

  日本水産学会 平成4年度日本水産学会 近畿支部例会 (京都)  1992/10
• 10) イシダイの酸素ストレスに対する生理的適応とビタミンＣの投与効果  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗

  日本水産学会 平成4年度日本水産学会春季大会 （東京）  1992/04
• 9) イシダイの成熟，産卵および卵質に及ぼすアスコルビン酸投与の効果  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 平成3年度日本水産学会春季大会 （岩手）  1991/10
• 8) イシダイの慢性酸素ストレス耐性に及ぼすビタミンＣ投与の効果  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 平成3年度日本水産学会春季大会 (東京)  1991/04
• 7) 魚類の酸素ストレス耐性とビタミンＣ  [Not invited]

  石橋 泰典; 家戸 敬太郎; 池田 静徳; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 平成2年度日本水産学会秋季大会 （奈良）  1990/11
• 5) 養成したクロマグロ，カツオおよびスマとそれぞれの天然魚との肝臓ビタミンD3 含量の比較  [Not invited]

  竹内 敦子; 小林 正; 池田 静徳; 石橋 泰典; 村田 修; 乗田 孝雄; 原田 輝雄

  日本水産学会 平成元年度日本水産学会秋季大会 （宮崎）  1989/10
• 6) マダイの通常発生２倍体と３倍体との体成分組成等の比較  [Not invited]

  原田 輝雄; 矢田 茂; 石橋 泰典; 村田 修; 宮下 盛; 那須 敏朗; 池田 静徳

  日本水産学会 平成元年度日本水産学会秋季大会 （宮崎）  1989/10
• 4) 養成したクロマグロ，カツオおよびスマとそれぞれの天然魚との一般成分，脂質および脂肪酸組成の比較  [Not invited]

  池田 静徳; 石橋 泰典; 村田 修; 乗田 孝雄; 那須 敏朗; 原田 輝雄

  日本水産学会 平成元年度日本水産学会春季大会 （東京）  1989/04
• 3) イシダイ稚魚のビタミンＣ要求量  [Not invited]

  池田 静徳; 石橋 泰典; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 昭和63年度日本水産学会秋季大会 （静岡）  1988/10
• 2) イシダイ稚魚の水溶性ビタミン要求  [Not invited]

  池田 静徳; 石橋 泰典; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 昭和63年度日本水産学会春季大会 （東京）  1988/04
• 1) イシダイ用試験飼料の基本組成の検討  [Not invited]

  池田 静徳; 石橋 泰典; 村田 修; 那須 敏朗; 原田 輝雄

  日本水産学会 昭和62年度日本水産学会秋季大会 (函館）  1987/10

Works

• 動植物融合型食糧・エネルギー生産システムの開発

  石橋 泰典 2010/04 - Today
• クロマグロ等の難種苗生産種の飼育技術開発に関する研究

  石橋 泰典 1998/04 - Today
• 閉鎖循環型陸上養殖システムの開発

  石橋 泰典 1994/04 - Today
• 養殖魚のストレスと健康に関する研究

  石橋 泰典 1990/04 - Today
• Study on the development of recirculating aquaculture system of freshwater fish

  2003 -2004
• Effects of feed additive on stress tolerance in marine fish

  2003 -2004
• Study on the development of recirculating aquaculture system of marine fish

  2001 -2002
• Study on apoptosis in fish larva

  2000
• Study on avoidance behavior in fish

  1997 -1998
• Study on stability of ascorbic acid in diet

  1996 -1997
• 養殖魚の飼料栄養要求に関する研究

  石橋 泰典 1986/04 -1992/03

MISC

• 12) クロマグロの持続的利用に向けた漁業技術研究 4.種苗生産の各種技術的課題と対策，取り組み

  石橋 泰典  日本水産学会誌  83-  (4)  688  -691  2017  [Invited]
  
• The Utilization of Artificial Seawater and the Requirement of Seawater-Derived Elements in Marine Larvae Culture.

  ISHIBASHI Yasunori  Bull. Soc. Sea Water Sci., Jpn  69-  (4)  255  -261  2015  [Invited]
  
• Cloning of the opsin gene in longtooth grouper (Epinephelus bruneus)

  Taro Matsumoto; Yasunori Ishibashi  INTEGRATIVE AND COMPARATIVE BIOLOGY  52-  E291  -E291  2012/04
• Current state and problem of seedling production in Pacific bluefin tuna

  Yasunori Ishibashi  Youshoku  609-  (10)  46  -50  2011/08  [Invited]
  
• The industrial mass production of artifical seedling in Pacific bluefin tuna Thunnus orientalis

  Yasunori Ishibashi  Kagaku to Seibutsu  48-  (5)  308  -310  2010/05  [Invited]
  
• Mass death and the countermeasure in seedling production of Pacific bluefin tuna Thunnus orientalis

  Yasunori Ishibashi  Aquanet  13-  (4)  30  -34  2010/04  [Invited]
  
• Ontogenic Changes of Visual Acuity in the Ocellate Puffer, Takifugu rubripes

  SUZUKI Takashi; ISHIBASHI Yasunori  水産増殖 = The aquiculture  56-  (1)  67  -71  2008/03
• Environmental physiology of cultivated fish.

  Ishibashi Y; Matsuura R; Suzuki T; Matsumoto T  The 21st Century COE Program Kinki University "Center of Aquaculture Science and Technology for Bluefin Tuna and Other Cultivated Fish"  3-  (14)  2008/03
• Stress response and habituation in cultivated fish exposed to environmental changes

  Yasunori Ishibashi  Aquanet  9-  (5)  24  -27  2006/05  [Invited]
  
• The Development of Visual Acuity in Pacific bluefin Tuna

  Shinsuke Torisawa; Tsutomu Takagi; Yasunori Ishibashi; Yoshifumi Sawada; Takeshi Yamane  Memoirs of the faculty of agriculture of Kinki University  38-  182  -186  2005
• Stress and the recovery from stress status in cultivated fish

  Yasunori Ishibashi  Aquanet  6-  (10)  18  -22  2003/10  [Invited]
  
• Diurnal changes of oxygen budgets in Chlorophyta Ulva pertusa

  Hirata H; Niiro M; Ishibashi Y; Kumai H  Mem. Fac. Agr. Kinki Univ  (35)  25  -29  2002
• Studies on the ascorbic acid requirements of cultivated marine fish. Bull.

  ISHIBASHI Yasunori  Fish Lab. Kinki Univ  4-  1  -100  1994
• 2.魚類の酸素ストレス耐性に及ぼすアスコルビン酸投与の効果 : ビタミンC研究委員会第76回会議研究発表要旨

  池田 静徳; 石橋 泰典; 家戸 敬太郎; 村田 修; 那須 敏朗  ビタミン  66-  (3)  214  -214  1992/03
• 2.イシダイ稚魚の成長と健康に及ぼす飼料ビタミンCレベルの影響(第69回会議研究発表要旨,ビタミンC研究委員会)

  池田 静徳; 石橋 泰典; 村田 修; 那須 敏朗; 原田 輝雄  ビタミン  63-  (10)  520  -520  1989/10
• イシダイ稚魚の水溶性ビタミン要求 : ビタミンCを中心に : ビタミンC研究委員会 : 第66回会議研究発表要旨

  池田 静徳; 石橋 泰典; 村田 脩; 那須 敏朗; 原田 輝雄  ビタミン  62-  (9)  527  -527  1988/09

Research Grants & Projects

• 魚類の光誘導による革新的行動制御法の開発

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2021/04 -2026/03 

  Author : 石橋 泰典
• 15) 平成29年度-令和2年度 ウナギ種苗の商業化に向けた大量生産システムの実証事業

  農水省委託事業：受託研究

  Date (from‐to) : 2017/04 -2021/03
• 14) 平成29年度-令和1年度 動植物融合型の食糧・エネルギー生産モデルの開発

  学内研究助成：21世紀研究開発奨励

  Date (from‐to) : 2018/04 -2020/03 

  Author : 石橋 泰典
• 13) 平成26-28年度 難種苗生産魚種の飼育環境制御による革新的生産システムの開発

  文部科学省科学研究助成 基盤研究(C)：

  Date (from‐to) : 2014/04 -2016/03 

  Author : 石橋 泰典
• 12) 平成26-28年度 魚類の完全養殖の高度化

  文部科学省科学研究助成 私立大学戦略的研究基盤形成支援事業：

  Date (from‐to) : 2014/04 -2016/03
• 11) 平成24-28年度 天然資源に依存しない持続的な養殖生産技術の開発-３クロマグロ高品質稚魚の供給技術の開発

  農水省委託事業 研究開発支援事業・制度：

  Date (from‐to) : 2012 -2016
• 10) 平成23-27年度 資源の持続的利用に向けたマグロ類2種の産卵生態と初期生活史に関する基礎研究

  SATREPS 地球規模課題対応国際科学技術協力 JST,JICA：

  Date (from‐to) : 2011 -2015
• 9) 平成20-24年度 クロマグロ等の養殖科学の国際研究拠点

  文部科学省 グローバルＣＯＥプログラム：

  Date (from‐to) : 2008 -2012
• 8) 平成15-19年度 クロマグロ等の魚類養殖産業支援型研究拠点

  文部科学省 ２１世紀ＣＯＥプログラム：

  Date (from‐to) : 2003 -2007
• The cultivation method of healthy marine fish seedlings using the heat shock factor as an indicator

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2003 -2006 

  Author : ISHIBASHI Yasunori

   

  Some indicators of environmental stressors were examined in the development of healthy fish seedling production. In addition, ontogenic changes in the stress response to environmental stressors were studied in some kinds of fish in their lifespan from larvae through the juvenile stage. At first, Pagrus major juveniles were subjected to low oxygen, high and low temperatures, hypo-and hyper-salinity. The specific activities of heat shock factor (HSF), glucocorticoid receptor (GR) and cortisol level as stress indicators in the fish were compared. The cortisol concentration was increased just after stress exposure, but the specific activities of HSF and GR gradually tended to become elevate. Fish showing both high and low tolerance to stress exposure were also selected and determined to be stress indicators. HSF and GR activities in high tolerance fish tended to be higher than those in the low tolerance fish. The HSF, GR and cortisol level were suggested to be good indicators to check in fish. Next, the ontogeny of hypoxia tolerance and stress response were studied in Pagrus major, Paralichthys olivaceus, Takifugu rubripes and Thunnus orientalis larvae and juveniles. The results indicated that around the stages of flexion and post-flexion larvae in fish, metabolic rates and/or energy metabolism were the highest, and consequently hypoxia tolerance was the lowest. Finally, ontogenic changes in some stress tolerances and the response to this were examined in larvae and juveniles. It was found that at the early juvenile stage all of the tolerances and the cortisol level were increased. The effects of cortisol immersion on various stress tolerances in larvae were also tested. The response of the survival rate, cortisol concentration and GR activity to stressors showed a tendency to increase as a result of cortisol immersion. It was therefore suggested that cortisol has an important role in adaptation to environmental stressors.
• 7) 平成15-18年度 ストレスタンパク質転写因子を用いた海産魚の健苗育成法

  文部科学省科学研究助成 基盤研究(C)：

  Date (from‐to) : 2003 -2006 

  Author : 石橋 泰典
• 6) 平成14年-18年度 クロマグロの資源増殖に関する生物・生化学的研究

  文部科学省科学研究助成 基盤研究(S)：

  Date (from‐to) : 2002 -2006
• 5) 平成15-16年度 海産魚のストレス耐性，抗病性に及ぼす多糖類投与の影響

  受託研究：

  Date (from‐to) : 2003 -2004 

  Author : 石橋 泰典
• 4) 平成15年度 海産種苗における環境ストレス適応能の発達様式

  学内研究助成 奨励研究：

  Date (from‐to) : 2003 -2003 

  Author : 石橋 泰典
• 3) 平成14年度 魚類の閉鎖循環式陸上養殖システムの開発に関する研究

  受託研究：

  Date (from‐to) : 2002 -2003 

  Author : 石橋 泰典
• 2) 平成13年度 ストレスタンパク質転写因子を用いた水環境評価法開発に関する研究

  クリタ水･環境科学振興財団：

  Date (from‐to) : 2001 -2002 

  Author : 石橋 泰典
• 1) 平成12年-13年度 海産種苗のストレス反応と健全性評価方法の検討

  文部科学省科学研究助成 奨励研究(A)：

  Date (from‐to) : 2000 -2001 

  Author : 石橋 泰典
• Ecological studies on the polyculture of fauna and flora

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 1993 -1995 

  Author : HIRATA Hachiro; YAMASAKI Shigehisa; ISHIBASHI Yasunoro

   

  Polyculture of fish and seaweeds is a benefical system in aquaculture. In spite of its importance from the ecological point of view, little attention has been given in polyculture system. This may be due to the fact that fish and seaweeds have different growth seasons ; fish grow in summer nd autum while seaweeds propagate in winter and spring. Previously, we found the culture techniques of sterile Ulva pertusa which grow in summer and autum as same seasons as fish growth. In the presesnt study, we have examined the polyculture of fish and seaweed, sterile Ulva pertusa. The first experiment was conducted in the fish cage culture farm from August 1993 to February 1994 at the Azumacho Fish Seedling Center. Two cages, 7x7x7m, were employed, one for polyculture experiment and the other one for control experiment by the routine method. Three years old red sea bream Pagrus major, about 1750 individuals each, were cultured in each cage. The Chlorophyta, steriled Ulva pertusa was cultured by the floating net system in the experimental cage. Dissolved oxygen and carbon dioxide were measured by Radiometer ABL-330. Considering the value of pO2 in the control cage as 100%, pO2 in the experiment cge stands 109.1%. On the other hand, average pCO2 in the control cage as 100%, pCO2 in the experiment cage stands 96.1%. From the results mentioned above, it may concluded that the pCO2 in the experimental cage decreased to 4% while pO2 augmented up to 9% compared to those in the control cage. The second experiment was also conducted in order to know the efficiences of light intensity and water temperture on growth of sterile Ulva pertusa as a foundamental study of "harmonized fish culture with environment" where substances were recycled through the alga. Tendency of low light and high light intensities were observed around the winter solstice and summer solstice, respectively. The highest value was 19.7 mol/m2/day around the winter silstice and 47.3 mol/m2/day around the summer solstice. In multiple regression analysis, multiple regression coefficient of 0.74 was obtained.

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