KINDAI UNIVERSITY


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YAMAMOTO Tetsushi

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FacultyDepartment of Pharmacy
PositionLecturer
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/429-yamamoto-tetsushi.html
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Last Updated :2020/08/10

Education and Career

Academic & Professional Experience

  •   2018 04 ,  - 現在, Kindai University
  •   2013 04 ,  - 2018 03 , Kindaii University
  •   2007 01 ,  - 2013 03 , Medical School, Nippon Medical School

Research Activities

Research Areas

  • Life sciences, Gastroenterology
  • Life sciences, Digestive surgery

Research Interests

  • MMP-9

Published Papers

  • Retinal proteomic evaluation of rats following streptozotocin-injection using shotgun proteomics, Hiroko Otake, Tetushi Yamamoto, Saori Deguchi, Atushi Taga, Noriaki Nagai, MOLECULAR MEDICINE REPORTS, MOLECULAR MEDICINE REPORTS, 21(1), 379 - 386, Jan. 2020 , Refereed
    Summary:It is important to elucidate how retinal stimulation leads to retinal protection and dysfunction. The current study aimed to identify factors that are up- and downregulated in the retinas of streptozotocin (STZ)-induced diabetic rats with acute retinal dysfunction. Retinal function was measured and changes in protein expressions were determined using electroretinograms (ERGs) and liquid chromatography/mass spectroscopy-based shotgun proteomics, respectively. The results revealed that the plasma glucose levels of STZ rats were markedly higher when compared with normal rats. Furthermore, levels of a-waves, b-waves and oscillatory potential amplitudes on ERGs in STZ rats were decreased compared with healthy animals. With use of shotgun proteomics, 391 proteins were identified in the retinas of normal rats and 541 proteins were found in the retinas of STZ rats. Of the 560 proteins identified in rat retinas, 372 (66.4%) were present in both normal and STZ rats. Of these, 19 (3.39%) were unique to normal rats and 169 (30.1%) were unique to STZ rats. Gene Ontology analysis was performed on the candidate proteins that were differentially regulated in the retinas of STZ rats and focused on those classified as 'protein binding', which serve important roles in retinal neurodegeneration. The results revealed an excessive expression of retinol-binding protein 1 (RBP1) and a negative expression of rod outer segment membrane protein 1 (Rom-1) in the retinas of STZ rats. Therefore, retinal function may be decreased with STZ-induced injury, and expressions of Rom-1 and RBP1 may be altered in the retinas of STZ rats.
  • Dark-colored maple syrup treatment induces S-phase cell cycle arrest via reduced proliferating cell nuclear antigen expression in colorectal cancer cells., Yamamoto T, Nishita T, Taga A, Oncology letters, Oncology letters, 17(3), 2713 - 2720, Mar. 2019 , Refereed
  • Collagen peptides from soft‑shelled turtle induce calpain‑1 expression and regulate inflammatory cytokine expression in HaCaT human skin keratinocytes., Yamamoto T, Nakanishi S, Mitamura K, Taga A, International journal of molecular medicine, International journal of molecular medicine, 42(2), 1168 - 1180, Aug. 2018 , Refereed
  • 2-Deoxy-d-glucose increases GFAT1 phosphorylation resulting in endoplasmic reticulum-related apoptosis via disruption of protein N-glycosylation in pancreatic cancer cells., Ishino K, Kudo M, Peng WX, Kure S, Kawahara K, Teduka K, Kawamoto Y, Kitamura T, Fujii T, Yamamoto T, Wada R, Naito Z, Biochemical and biophysical research communications, Biochemical and biophysical research communications, 501(3), 668 - 673, Jun. 2018 , Refereed
  • Instillation of Sericin Enhances Corneal Wound Healing through the ERK Pathway in Rat Debrided Corneal Epithelium., Nagai N, Fukuoka Y, Ishii M, Otake H, Yamamoto T, Taga A, Okamoto N, Shimomura Y, International journal of molecular sciences, International journal of molecular sciences, 19(4), Apr. 2018 , Refereed
  • Proteomic profile of the lens in a streptozotocin-induced diabetic rat model using shotgun proteomics., Nagai N, Yamamoto T, Mitamura K, Taga A, Biomedical reports, Biomedical reports, 7(5), 445 - 450, Nov. 2017 , Refereed
  • Shotgun label-free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft-shelled turtle., Yamamoto T, Nakanishi S, Mitamura K, Taga A, Journal of biomedical materials research. Part B, Applied biomaterials, Journal of biomedical materials research. Part B, Applied biomaterials, 106(6), 2403 - 2413, Nov. 2017 , Refereed
  • Effect of dark-colored maple syrup on cell proliferation of human gastrointestinal cancer cell., Yamamoto T, Sato K, Kubota Y, Mitamura K, Taga A, Biomedical reports, Biomedical reports, 7(1), 6 - 10, Jul. 2017 , Refereed
  • Analysis of protein expression regulated by lumican in PANC-1 cells using shotgun proteomics, Tetsushi Yamamoto, Mitsuhiro Kudo, Wei-Xia Peng, Zenya Naito, ONCOLOGY REPORTS, ONCOLOGY REPORTS, 30(4), 1609 - 1621, Oct. 2013 , Refereed
    Summary:Lumican, a member of the class II small leucine-rich proteoglycan family, regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. We previously reported that lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma (PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. Lumican stimulates growth and inhibits the invasion of a PDAC cell line. In the present study, we performed a global shotgun proteomic analysis using lumican-overexpressing PANC-1 cells and lumican downregulated PANC-1 cells to identify candidate proteins that are regulated by lumican and related to cell growth and invasion in PDAC cells. A total of 448 proteins were identified from lumican-overexpressing PANC-1 and control cells. Additionally, 451 proteins were identified from lumican-downregulated PANC-1 cells and control cells. As a result of semi-quantification based on spectral counting, 174 differentially expressed proteins were identified by lumican upregulation, and 143 differentially expressed proteins were identified by lumican downregulation. The expression levels of 24 proteins, including apoptosis-and invasion-related proteins correlated with lumican expression levels. It is likely that the expression of these proteins is regulated by lumican, and that they are involved in apoptosis and invasion in PDAC. These findings suggest that lumican may be involved in cell growth and invasion through the regulation of these 24 proteins expressed in PDAC.
  • Expression of Lumican in Hidroacanthoma Simplex and Clonal-Type Seborrheic Keratosis as a Potent Differential Diagnostic Marker., Takayama R, Ansai SI, Ishiwata T, Yamamoto T, Matsuda Y, Naito Z, Kawana S, The American Journal of dermatopathology, The American Journal of dermatopathology, May 2013 , Refereed
  • Morphological and cytoskeletal changes of pancreatic cancer cells in three-dimensional spheroidal culture, MATSUDA Yoko, ISHIWATA Toshiyuki, KAWAMOTO Yoko, KAWAHARA Kiyoko, PENG Wei-Xia, YAMAMOTO Tetsushi, NAITO Zenya, 43(4), 211 - 217, Dec. 01 2010 , Refereed
  • A method for detecting tumor cells derived from colorectal cancer by targeting cell surface glycosylation with affinity capillary electrophoresis., Tetsushi Yamamoto, Kanta Sato, Shinpei Wakahara, Kuniko Mitamura, Atsushi Taga, Journal of pharmaceutical and biomedical analysis, Journal of pharmaceutical and biomedical analysis, 182, 113138 - 113138, Apr. 15 2020 , Refereed
    Summary:Circulating tumor cells (CTCs) are involved in metastasis; thus, one of the most important approaches for identifying metastatic cancer is to detect CTCs in blood. In the present study, we examined whether directly analyzing cells with capillary electrophoresis (CE) could distinguish cancer cells from normal cells, based on differences in cell surface glycosylation. We compared human colorectal cancer (CRC) cell lines to a normal colon epithelium cell line. Our results demonstrated that direct CE analysis could successfully distinguish between CRC and normal cells with high reproducibility, based on migration times. We found that the weighted-average migration time was significantly shorter for CRC cells than for normal cells. Next, we observed changes in the electrophoretic behaviors of CRC cells by adding five different types of lectins. When Aleuria aurantia lectin was added, migration delays were observed in CRC cells, but not in normal colon cells. Therefore, by focusing on shifts in migration time after adding specific lectins, we could distinguish cancer cells from normal cells. These findings suggested that this diagnostic method of directly analyzing cells with CE after adding specific lectin(s) could be useful for detecting the difference in the sugar moieties on a surface of normal and cancer cells.
  • Cyclophilin a knokdown inhibits cell migration and invasion through the suppression of epithelial-mesenchymal transition in colorectal cancer cells., Tetsushi Yamamoto, Hideki Takakura, Kuniko Mitamura, Atsushi Taga, Biochemical and biophysical research communications, Biochemical and biophysical research communications, Mar. 16 2020 , Refereed
    Summary:Enhanced expression of cyclophilin A (CypA) in colorectal cancer (CRC) was reported; however, how CypA influences CRC progression is not clear. Therefore, we examine the effects of CypA on CRC cell progression. Knockdown of CypA in SW480 cells significantly inhibited cell migration and invasion but had no effect on cell proliferation. In addition, upregulation of E-cadherin and downregulation of N-cadherin and Snail expression were observed by CypA knockdown. These results suggested that CypA knockdown inhibited cell migration and invasion by suppressing epithelial-mesenchymal transition. CypA knockdown was also associated with increased p38 phosphorylation, and the p38 inhibitor treatment led to increase in the number of invasive CypA-knockdown SW480 cells. Therefore, CypA may be a potential therapeutic target in preventing CRC metastasis.
  • Identification of a Novel Oligosaccharide in Maple Syrup as a Potential Alternative Saccharide for Diabetes Mellitus Patients., Kanta Sato, Noriaki Nagai, Tetsushi Yamamoto, Kuniko Mitamura, Atsushi Taga, International journal of molecular sciences, International journal of molecular sciences, 20(20), Oct. 11 2019 , Refereed
    Summary:The incidence of diabetes mellitus (DM) is increasing rapidly and is associated with changes in dietary habits. Although restrictions in the use of sweeteners may prevent the development of DM, this might reduce the quality of life of patients with DM. Therefore, there has been a great deal of research into alternative sweeteners. In the search for such sweeteners, we analyzed the carbohydrate content of maple syrup and identified a novel oligosaccharide composed of fructose and glucose, linked at the C-4 of glucose and the C-6 of fructose. This oligosaccharide inhibited the release of fructose from sucrose by invertase (IC50: 1.17 mmol/L) and the decomposition of maltose by α-(1-4) glucosidase (IC50: 1.72 mmol/L). In addition, when orally administered together with sucrose to rats with DM, the subsequent plasma glucose concentrations were significantly lower than if the rats had been administered sucrose alone, without having any effect on the insulin concentration. These findings suggest that this novel oligosaccharide might represent a useful alternative sweetener for inclusion in the diet of patients with DM and may also have therapeutic benefits.
  • A Proteomic Approach for Understanding the Mechanisms of Delayed Corneal Wound Healing in Diabetic Keratopathy Using Diabetic Model Rat, Tetsushi Yamamoto, Hiroko Otake, Noriko Hiramatsu, Naoki Yamamoto, Atsushi Taga, Noriaki Nagai, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(11), Nov. 2018 , Refereed
    Summary:Diabetes mellitus is a widespread metabolic disorder, and long-term hyperglycemia in diabetics leads to diabetic keratopathy. In the present study, we used a shotgun liquid chromatography/mass spectrometry-based global proteomic approach using the cornea of streptozotocin-induced diabetic (STZ) rats to examine the mechanisms of delayed corneal wound healing in diabetic keratopathy. Applying a label-free quantitation method based on spectral counting, we identified 188 proteins that showed expression changes of >2.0-fold in the cornea of STZ rats. In particular, the level of lumican expression in the cornea of STZ rats was higher than that of the normal rats. In the cornea of the normal rat, the expression level of lumican was elevated during the wound healing process, and it returned to the same expression level as before cornea injury after the wound was healed completely. On the other hand, a high expression level of lumican in the cornea of STZ rats was still maintained even after the wound was healed completely. In addition, adhesion deficiency in corneal basal cells and Bowman's membrane was observed in the STZ rat. Thus, abnormally overexpressed lumican may lead to adhesion deficiency in the cornea of STZ rats.
  • Increased expression of PDIA3 and its association with cancer cell proliferation and poor prognosis in hepatocellular carcinoma, Hideyuki Takata, Mitsuhiro Kudo, Tetsushi Yamamoto, Junji Ueda, Kousuke Ishino, Wei-Xia Peng, Ryuichi Wadai, Nobuhiko Taniai, Hiroshi Yoshida, Eiji Uchida, Zenya Naito, ONCOLOGY LETTERS, ONCOLOGY LETTERS, 12(6), 4896 - 4904, Dec. 2016 , Refereed
    Summary:The prognosis of hepatocellular carcinoma (HCC) is unfavorable following complete tumor resection. The aim of the present study was to identify a molecule able to predict HCC prognosis through comprehensive protein profiling and to elucidate its clinicopathological significance. Comprehensive protein profiling of HCC was performed by liquid chromatography-tandem mass spectrometry. Through the bioinformatic analysis of proteins expressed differentially in HCC and non-HCC tissues, protein disulfide-isomerase A3 (PDIA3) was identified as a candidate for the prediction of prognosis. PDIA3 expression was subsequently examined in 86 cases of HCC by immunostaining and associations between PDIA3 expression levels and clinicopathological characteristics were evaluated. The Ki-67 index and apoptotic cell death of carcinoma cells were examined by immunostaining and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay in 24 cases. The results demonstrated that PDIA3 was expressed in all 86 HCC cases; 56 HCC cases (65%) exhibited high expression of PDIA3 and 30 (35%) exhibited low expression. The disease-free and overall survival times of HCC patients with high PDIA3 expression were significantly shorter than in HCC patients with low expression. Furthermore, increased expression of PDIA3 was associated with an elevated Ki-67 index, indicating increased cancer cell proliferation and a reduction in apoptotic cell death. Taken together, these results suggest that PDIA3 expression is associated with tumor proliferation and decreased apoptosis in HCC, and that increased expression of PDIA3 predicts poor prognosis. PDIA3 may therefore be a key molecule in the development of novel targeting therapies for patients with HCC.
  • Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue, Tetsushi Yamamoto, Mitsuhiro Kudo, Wei-Xia Peng, Hideyuki Takata, Hideki Takakura, Kiyoshi Teduka, Takenori Fujii, Kuniko Mitamura, Atsushi Taga, Eiji Uchida, Zenya Naito, TUMOR BIOLOGY, TUMOR BIOLOGY, 37(10), 13595 - 13606, Oct. 2016 , Refereed
    Summary:Colorectal cancer (CRC) is one of the most common cancers worldwide, and many patients are already at an advanced stage when they are diagnosed. Therefore, novel biomarkers for early detection of colorectal cancer are required. In this study, we performed a global shotgun proteomic analysis using formalin-fixed and paraffin-embedded (FFPE) CRC tissue. We identified 84 candidate proteins whose expression levels were differentially expressed in cancer and non-cancer regions. A label-free semiquantitative method based on spectral counting and gene ontology (GO) analysis led to a total of 21 candidate proteins that could potentially be detected in blood. Validation studies revealed cyclophilin A, annexin A2, and aldolase A mRNA and protein expression levels were significantly higher in cancer regions than in non-cancer regions. Moreover, an in vitro study showed that secretion of aldolase A into the culture medium was clearly suppressed in CRC cells compared to normal colon epithelium. These findings suggest that decreased aldolase A in blood may be a novel biomarker for the early detection of CRC.
  • Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma, Akiko Kanzaki, Mitsuhiro Kudo, Shin-ichi Ansai, Wei-Xia Peng, Kousuke Ishino, Tetsushi Yamamoto, Ryuichi Wada, Takenori Fuji, Kiyoshi Teduka, Kiyoko Kawahara, Yoko Kawamoto, Taeko Kitamura, Seiji Kawana, Hidehisa Saeki, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 48(3), 1007 - 1015, Mar. 2016 , Refereed
    Summary:In the histopathological diagnosis of cutaneous tumors, the differential diagnosis of squamous cell carcinoma (SCC) with crateriform architecture and keratoacanthoma (KA) is often difficult so an accurate understanding of the biological features and the identification of reliable markers of SCC and KA are crucial issues. Insulin-like growth factor 2 mRNA-binding protein-3 (IGF2BP3, also known as IMP3) is thought of as a bona fide oncofetal protein, which is overexpressed and is involved in cell proliferation, migration, and invasion in several kinds of tumors. However, the role of IMP3 in cutaneous SCC and KA has not been well studied. Therefore, we focused on studying the biological functions of IMP3 in SCC and KA. In human skin SCC cell lines, HSC-1 and HSC-5, and the human keratinocyte cell line, HaCaT, IMP3 mRNA levels were significantly higher than that of normal human skin. The knockdown of IMP3 expression reduced the proliferation of HSC-1, and significantly reduced invasion by HSC-1 and HSC-5. In contrast, the knockdown of IMP3 did not significantly affect invasion by HaCaT cells. In immunohistochemical studies of SCC and KA tissues, the Ki-67 labeling index (LI) of the suprabasal cell layer was significantly higher in SCC, compared with KA tissues and the tumor-free margin (TFM) adjacent to SCC and KA. Most SCC tissues stained strongly positive for IMP3, but KA tissues and TFM were mostly negative for IMP3. The Ki-67 LI of the IMP3-positive group was significantly higher than that of the IMP3-negative group in the suprabasal cell layer of SCC. These results suggest that IMP3 plays an important role in proliferation and, more significantly, in the invasion of SCC, and may be a suitable marker for the histopathological diagnosis of SCC with a crateriform architecture and KA. Furthermore, IMP3 may potentially be a new therapeutic target for SCC.
  • Optimization of Method to Extract Collagen from "Emperor" Tissue of Soft-shelled Turtles, Tetsushi Yamamoto, Kentaro Uemura, Yuki Sawashi, Kuniko Mitamura, Atsushi Taga, JOURNAL OF OLEO SCIENCE, JOURNAL OF OLEO SCIENCE, 65(2), 169 - 175, Feb. 2016 , Refereed
    Summary:Soft-shelled turtles (Pelodiscus sinensis) are widely distributed in some Asian countries, and parts of this turtle contain abundant collagen. In this study, we optimized a method for extracting collagen from the soft-shelled turtle. We used three types of solvent and four extraction conditions to determine an effective collagen extraction method, which was extraction at 37 degrees C with acetic acid after hydrochloric acid pretreatment. Next, we extracted collagen from three regions in the soft-shelled turtle: muscle, skin, and an area of soft tissue in the periphery of the turtle shell known in Japan and China as the "emperor." We determined that emperor tissue yielded the highest concentration and purity of collagen. We then optimized the pretreatment method for extraction from emperor tissue by using formic acid instead of hydrochloric acid, and the amount of extracted collagen increased by approximately 1.3-fold. Finally, we identified the optimal solvent out of four types of organic acid for collagen extraction from emperor tissue; the amount of extracted collagen from emperor tissue increased approximately 3-fold when citric acid was used as the extraction solvent instead of acetic acid. Emperor tissue can regenerate; thus, it is possible to obtain collagen from the emperor repeatedly without killing the turtle. Our findings suggest that the emperor tissue of soft-shelled turtles may be a good source of collagen for pharmaceutical and cosmetic applications.
  • Peripheral HMGB1-induced hyperalgesia in mice: Redox state-dependent distinct roles of RAGE and TLR4, Daichi Yamasoba, Maho Tsubota, Risa Domoto, Fumiko Sekiguchi, Hiroyuki Nishikawa, Keyue Liu, Masahiro Nishibori, Hiroyasu Ishikura, Tetsushi Yamamoto, Atsushi Taga, Atsufumi Kawabata, JOURNAL OF PHARMACOLOGICAL SCIENCES, JOURNAL OF PHARMACOLOGICAL SCIENCES, 130(2), 139 - 142, Feb. 2016 , Refereed
    Summary:Nuclear HMGB1 that contains 3 cysteine residues is acetylated and secreted to the extracellular space, promoting inflammation via multiple molecules such as RAGE and TLR4. We thus evaluated and characterized the redox state-dependent effects of peripheral HMGB1 on nociception. Intraplantar (i.pl.) administration of bovine thymus-derived HMGB1 (bt-HMGB1), all-thiol HMGB1 (at-HMGB1) or disulfide HMGB1 (ds-HMGB1) caused long-lasting mechanical hyperalgesia in mice. The hyperalgesia following i.pl. bt-HMGB1 or at-HMGB1 was attenuated by RAGE inhibitors, while the ds-HMGB1-induced hyperalgesia was abolished by a TLR4 antagonist. Thus, nociceptive processing by peripheral HMGB1 is considered dependent on its redox states. (C) 2016 Japanese Pharmacological Society. Production and hosting by Elsevier B.V.
  • Cystatin B as a potential diagnostic biomarker in ovarian clear cell carcinoma, Akane Takaya, Wei-Xia Peng, Kousuke Ishino, Mitsuhiro Kudo, Tetsushi Yamamoto, Ryuichi Wada, Toshiyuki Takeshita, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 46(4), 1573 - 1581, Apr. 2015 , Refereed
    Summary:Epithelial ovarian cancer (EOC) consists of four major subtypes: clear cell carcinoma (CCC), endometrioid adenocarcinoma (EA), mucinous adenocarcinoma (MA) and serous adenocarcinoma (SA). Relative to the other subtypes, the prognosis of CCC is poor due to a high recurrence rate and chemotherapy resistance, but CCC-specific biomarkers have yet to be identified. With the aim of identifying diagnostic and treatment biomarkers for CCC, we analyzed 96 cases of EOC (32 CCC, 13 EA, 19 MA, 32 SA) using liquid chromatography/mass spectrometry (LC/MS) followed by immunohistochemistry (IHC) and quantitative reverse transcription PCR (RT-qPCR). Semi-quantification of protein differences between subtypes showed upregulation of 150 proteins and downregulation of 30 proteins in CCC relative to the other subtypes. Based on hierarchical clustering that revealed a marked distinction in the expression levels of cystatin B (CYTB) and Annexin A4 (ANXA4) in CCC relative to the other subtypes, we focused the study on CYTB and ANXA4 expression in E0Cs by IHC, RT-qPCR and western blot analyses using tissue specimens and cultured cells. As a result, compared to the other subtypes, CCC showed significantly high expression levels of CYTB and ANXA4 in the analyses. To examine the possibility of CYTB and ANXA4 as serum diagnostic biomarkers of CCC, we checked the protein levels in conditioned media and cell lysates using culture cells. Compared with the other subtypes, CCC cell lines showed a significantly higher level of expression of CYTB in both conditioned media and cell lysates, while ANXA4 showed a higher level of expression in cell lysates only. Our results demonstrate that CYTB and ANXA4 overexpression may be related to carcinogenesis and histopathological differentiation of CCC. CYTB may be a secreted protein, and may serve as a potential serum diagnostic biomarker of CCC, while ANXA4 may be useful as an intracellular marker.
  • Inhibitory effect of maple syrup on the cell growth and invasion of human colorectal cancer cells, Tetsushi Yamamoto, Kentaro Uemura, Kaho Moriyama, Kuniko Mitamura, Atsushi Taga, ONCOLOGY REPORTS, ONCOLOGY REPORTS, 33(4), 1579 - 1584, Apr. 2015 , Refereed
    Summary:Maple syrup is a natural sweetener consumed by individuals of all ages throughout the world. Maple syrup contains not only carbohydrates such as sucrose but also various components such as organic acids, amino acids, vitamins and phenolic compounds. Recent studies have shown that these phenolic compounds in maple syrup may possess various activities such as decreasing the blood glucose level and an anticancer effect. In this study, we examined the effect of three types of maple syrup, classified by color, on the cell proliferation, migration and invasion of colorectal cancer (CRC) cells in order to investigate whether the maple syrup is suitable as a phytomedicine for cancer treatment. CRC cells that were administered maple syrup showed significantly lower growth rates than cells that were administered sucrose. In addition, administration of maple syrup to CRC cells caused inhibition of cell invasion, while there was no effect on cell migration. Administration of maple syrup clearly inhibited AKT phosphorylation, while there was no effect on ERK phosphorylation. These data suggest that maple syrup might inhibit cell proliferation and invasion through suppression of AKT activation and be suitable as a phytomedicine for CRC treatment, with fewer adverse effects than traditional chemotherapy.
  • Changes in Plasma Glucose in Otsuka Long-Evans Tokushima Fatty Rats After Oral Administration of Maple Syrup, Noriaki Nagai, Tetsushi Yamamoto, Wataru Tanabe, Yoshimasa Ito, Satoshi Kurabuchi, Kuniko Mitamura, Atsushi Taga, JOURNAL OF OLEO SCIENCE, JOURNAL OF OLEO SCIENCE, 64(3), 331 - 335, Mar. 2015 , Refereed
    Summary:We investigate whether maple syrup is a suitable sweetener in the management of type 2 diabetes using the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. The enhancement in plasma glucose (PG) and glucose absorption in the small intestine were lower after the oral administration of maple syrup than after sucrose administration in OLETF rats, and no significant differences were observed in insulin levels. These data suggested that maple syrup might inhibit the absorption of glucose from the small intestine and preventing the enhancement of PG in OLETF rats. Therefore, maple syrup might help in the prevention of type 2 diabetes.
  • Lumican as a Novel Marker for Differential Diagnosis of Bowen Disease and Actinic Keratosis, Ryoko Takayama, Toshiyuki Ishiwata, Shin-ichi Ansai, Tetsushi Yamamoto, Yoko Matsuda, Zenya Naito, Seiji Kawana, AMERICAN JOURNAL OF DERMATOPATHOLOGY, AMERICAN JOURNAL OF DERMATOPATHOLOGY, 35(8), 827 - 832, Dec. 2013 , Refereed
    Summary:Lumican, a member of the small leucine-rich proteoglycan family, regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression correlates with pathological conditions, including skin fragility, corneal opacification, and corneal and cardiac wound healing. Lumican is overexpressed in tumor cells, including in the breast, colorectal, neuroendocrine cell, uterine cervical, and pancreatic cancers. Lumican expression also correlates with the growth and metastasis of various malignancies. For example, lumican expression is lower in the dermis of malignant melanoma cases than in early-stage melanomas. However, the expression patterns and roles of lumican in nonmelanoma skin cancer have not been elucidated. In this study, we used immunohistochemistry and in situ hybridization to examine the expression patterns of lumican in normal skin, Bowen disease, and actinic keratosis. In normal skin, lumican was expressed in the collagen fibers in the dermis, acrosyringium, follicular epithelium, and sebocytes but not in epidermal keratinocytes. In Bowen disease, lumican was expressed in 34 (91.8%) of 37 patients. Notably, all cases of actinic keratosis were negative for lumican. These findings suggest that lumican plays an important role in the pathogenesis of Bowen disease and actinic keratosis and might be useful as an adjunct to the diagnosis for subtypes of 2 diseases: bowenoid actinic keratosis and Bowen disease in sun-exposed areas.
  • Nodular fasciitis in the parotid gland: A case report and review of the literature, Wei-Xia Peng, Mitsuhiro Kudo, Tetsushi Yamamoto, Shunta Inai, Takenori Fujii, Kiyoshi Teduka, Kiyoko Kawahara, Zenya Naito, DIAGNOSTIC CYTOPATHOLOGY, DIAGNOSTIC CYTOPATHOLOGY, 41(9), 829 - 833, Sep. 2013 , Refereed
    Summary:Nodular fasciitis (NF) is a benign, reactive lesion with a self-limiting process. Because NF is rare in the parotid gland and has many cytological similarities to other benign or malignant tumors, cytological misinterpretation is common. The patient, a 30-year-old woman, had a painless mass in her right parotid gland. Fine needle aspiration cytology (FNAC) was performed. Spindle cells with basophilic and well-demarcated cytoplasm were observed in a mucoid-like background. The mucoid-like substance was metachromatic and appeared to be the matrix of PA. Histopathologically, spindle-shaped cells with intervening birefringent mature collagen were arranged in short irregular bundles. Prominent mucoid-like matrixes as well as few infiltrating neutrophils and lymphocytes were found in the background. Lesional cells were positive for CD10 and -catenin in the cytoplasm, but negative for cytokeratin, the S-100 protein, CD34, and neurofilament. Ultimately, this patient was diagnosed with NF. In FNAC of the parotid gland region, distinguishing NF from other real tumors is important for deciding treatment strategies. Diagn. Cytopathol. 2013;41:829-833. (c) 2013 Wiley Periodicals, Inc.
  • Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer, Atsuki Sato, Toshiyuki Ishiwata, Yoko Matsuda, Tetsushi Yamamoto, Hirobumi Asakura, Toshiyuki Takeshita, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 41(2), 441 - 448, Aug. 2012 , Refereed
    Summary:Nestin expression reportedly correlates with aggressive growth, metastasis, poor prognosis and presence of cancer stem cells (CSCs) in various tumors. In this study, we determined the expression and role of nestin in cervical intraepithelial neoplasia (CIN) and cervical cancer. We performed immunohistochemical and in situ hybridization analyses of nestin in 26 cases for each stage of CIN and 55 cervical cancer tissue samples. To examine the role of nestin in cervical cancer cells, we stably transfected expression vectors containing nestin cDNA into ME-180 cells. We studied the effects of increased nestin expression on cell proliferation, cell motility, invasion as well as sphere and soft agar formation. Nestin was not localized in the squamous epithelium in normal cervical tissues, but it was weakly expressed in the basal squamous epithelium of CIN 1. In CIN 2, nestin was localized to the basal to lower 2/3 of the squamous epithelium, whereas in CIN 3, it was localized to the majority of the squamous epithelium. Nestin was detected in all cases of invasive cervical cancer. Nestin mRNA was expressed in both ME-180 and CaSki cells. Growth rate, cell motility and invasion ability of stably nestin-transfected ME-180 cells were not different from empty vector-transfected ME-180 (mock cells). However, the nestin-transfected ME-180 cells formed more colonies and spheres compared to the mock cells. These findings suggest that nestin plays important roles in carcinogenesis and tumor formation of cervical cancer cells. Nestin may closely correlate with regulation of CSCs.
  • Secreted 70 kDa lumican stimulates growth and inhibits invasion of human pancreatic cancer, Tetsushi Yamamoto, Yoko Matsuda, Kiyoko Kawahara, Toshiyuki Ishiwata, Zenya Naito, CANCER LETTERS, CANCER LETTERS, 320(1), 31 - 39, Jul. 2012 , Refereed
    Summary:Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma (PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PANC-1 cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PANC-1 cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
  • Enhanced Expression of Fibroblast Growth Factor Receptor 2 IIIc Promotes Human Pancreatic Cancer Cell Proliferation, Toshiyuki Ishiwata, Yoko Matsuda, Tetsushi Yamamoto, Eiji Uchida, Murray Korc, Zenya Naito, AMERICAN JOURNAL OF PATHOLOGY, AMERICAN JOURNAL OF PATHOLOGY, 180(5), 1928 - 1941, May 2012 , Refereed
    Summary:In pancreatic ductal adenocarcinoma (PDAC), the fibroblast growth factor receptor 1 (FGFR-1) IIIb isoform correlates with the inhibition of cancer cell proliferation, migration, and invasion, whereas FGFR-1 IIIc enhances cancer cell proliferation. The FGFR-2 IIIb isoform is expressed in PDAC, and its expression correlates with increased venous invasion. We examined the role of FGFR-2 IIIc in PDAC. FGFR-2 IIIc was expressed in all six pancreatic cancer cell lines examined and was highest in PANC-1 cells. FGFR-2 IIIc was abundant in the cancer cells from 83 of 117 PDAC cases, which correlated with decreased duration to development of liver metastasis after surgery. FGFR-2 IIIc-transfected cells exhibited increased proliferation in vitro and formed larger subcutaneous and orthotopic tumors, the latter producing more liver metastases. Moreover, FGF-2 exerted a more rapid stimulatory effect on the levels of phosphorylated extracellular signal-regulated kinase (p-ERK) in FGFR-2 IIIc stably transfected PANC-1 cells, compared with control cells. FGFR-2 IIIc-transfected cells also formed more spheres and contained more side population cells. Suppression of FGFR-2 IIIc expression inhibited the proliferation of PANC-1 cells, whereas an anti-FGFR-2 IIIc antibody inhibited the proliferation and migration of PANC-1 cells. Thus, high FGFR-2 IIIc levels in PDAC contribute to disease aggressiveness and confer to pancreatic cancer cells features suggestive of cancer stem cells, indicating that FGFR-2 IIIc may be a novel and important therapeutic target in PDAC. (Am J Pathol 2012,180:1928-1941; DOI: 10.1016/j.ajpath.2012.01.020)
  • Expression of cancer stem cell markers in pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias, Shoko Kure, Yoko Matsuda, Masahito Hagio, Taeko Suzuki, Junji Ueda, Kazuya Yamahatsu, Tetsushi Yamamoto, Zenya Naito, Toshiyuki Ishiwata, CANCER RESEARCH, CANCER RESEARCH, 72, Apr. 2012 , Refereed
  • Role of stem cell marker, nestin in human uterine cervical intraepithelial neoplasia and cervical cancer, Atsuki Sato, Toshiyuki Ishiwata, Tetsushi Yamamoto, Yoko Matsuda, Hirobumi Asakura, Toshiyuki Takeshita, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 72, Apr. 2012 , Refereed
  • Lumican as a novel differential diagnostic maker for Bowen's disease and actinic keratosis, Ryoko Takayama, Toshiyuki Ishiwata, Shin-ichi Ansai, Tetsushi Yamamoto, Yoko Matsuda, Seiji Kawana, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 72, Apr. 2012 , Refereed
  • Keratinocyte growth factor stimulates growth of MIA PaCa-2 cells through extracellular signal-regulated kinase phosphorylation, Tetsushi Yamamoto, Yoko Matsuda, Kiyoko Kawahara, Zenya Naito, Toshiyuk Ishiwata, ONCOLOGY LETTERS, ONCOLOGY LETTERS, 3(2), 307 - 310, Feb. 2012 , Refereed
    Summary:Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7, is mainly synthesized by mesenchymal cells. KGF modulates proliferation, differentiation, migration and adhesion to extracellular matrices of epithelial cells that specifically express the KGF receptor (KGFR). We previously reported that KGF is expressed in cancer cells and adjacent stromal fibroblasts in human pancreatic cancer tissues. Furthermore, KGF is thought to stimulate the growth of certain pancreatic cancer cell lines. The aim of the present study was to examine whether the mitogen-activated protein kinase (MAPK) pathway contributes to exogenous KGF-induced pancreatic cancer cell growth. Recombinant human KGF (rhKGF) was administered to MIA PaCa-2 cells, which expressed KGFR and negligible levels of KGF. Cell growth rates in MIA PaCa-2 cells were significantly increased in a dose-dependent manner following the addition of rhKGF. In the MAPK pathway, phosphorylation of extracellular signal-regulated kinase (ERK) in MIA PaCa-2 cells was increased in a dose-dependent manner, and phosphorylation of p38 was slightly increased following the administration of 100 ng/ml rhKGF. In contrast, JNK was not phosphorylated following the addition of rhKGF in MIA PaCa-2 cells. U0126, a specific inhibitor of ERK activation, decreased the rhKGF-induced phosphorylation of ERK and the growth rates of MIA PaCa-2 cells. These findings indicated that phosphorylation of the ERK signaling pathway plays a significant role in exogenous KGF-induced pancreatic cancer cell growth.
  • Overexpressed fibroblast growth factor receptor 2 in the invasive front of colorectal cancer: A potential therapeutic target in colorectal cancer, Yoko Matsuda, Toshiyuki Ishiwata, Kazuya Yamahatsu, Kiyoko Kawahara, Masahito Hagio, Wei-Xia Peng, Tetsushi Yamamoto, Nando Nakazawa, Tomoko Seya, Yoshiharu Ohaki, Zenya Naito, CANCER LETTERS, CANCER LETTERS, 309(2), 209 - 219, Oct. 2011 , Refereed
    Summary:Fibroblast growth factor receptor 2 (FGFR2) is considered a novel therapeutic target for various cancer. We used a silencing strategy to clarify the effect of reduced FGFR2 expression in human colorectal cancer (CRC) cells. The invasive front of cancer cells exhibited stronger FGFR2 expression than the surface area of the cancers. FGFR2 shRNA-transfected LoVo cells inhibited cell migration, invasion and tumor growth in vitro and in vivo. Thus, FGFR2 plays important roles in CRC progression in association with tumor cell migration, invasion and growth, and FGFR2 might be a novel therapeutic target for CRC. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
  • Neuroepithelial stem cell marker nestin regulates the migration, invasion and growth of human gliomas, Toshiyuki Ishiwata, Kiyoshi Teduka, Tetsushi Yamamoto, Kiyoko Kawahara, Yoko Matsuda, Zenya Naito, ONCOLOGY REPORTS, ONCOLOGY REPORTS, 26(1), 91 - 99, Jul. 2011 , Refereed
    Summary:Nestin, a class VI intermediate filament protein, was originally described as a neuronal stem cell marker during central nervous system development. Nestin is expressed in gliomas, and its expression levels are higher in gliomas with high WHO histopathological classification grades than in those with low grades. In the present study, we examined whether nestin regulates the biological activities of human glioma cells. Immunohistochemically, the nestin expression patterns in 10 human glioblastoma patients were examined. The expression levels of nestin in A172, a human high-grade glioma cell line, and KG-I-C, a human low-grade glioma cell line, were examined using real-time PCR, Western blot and immunofluorescence analyses. An expression vector carrying a short hairpin RNA targeting nestin was stably transfected into A172 (Sh) cells. The effects of decreased expression levels of nestin in Sh cells on cell growth, migration, invasion, adhesion to extracellular matrices and fibrillar actin expression on three-dimensional culture plates were examined. The nestin expression vector was transiently transfected into KG-1-C (Nes) cells, and the effects of the nestin overexpression on cell growth and migration were examined. Nestin was expressed in the cytoplasm of the glioblastoma cells in all cases examined. Sh cells showed marked decreases in the expression levels of nestin mRNA and protein, and the growth rate of Sh cells was lower than that of sham (Sc) cells. In contrast, the adhesion activity of Sh cells to types I and IV collagens, fibronectin and laminin was higher than that of Sc cells. Fibrillar actin was clearly detected at the periphery of colonies of Sh cells at the attachment sites on three-dimensional culture plates. The migration and invasion of Sh cells were markedly inhibited compared with those of Sc cells. In contrast, the levels of nestin expression markedly increased in the Nes cells, which were transiently transfected with the nestin expression vector. The growth rate and motility of Nes cells were higher than those of the mock cells. In conclusion, nestin plays important roles in cell growth, migration, invasion and adhesion to extra-cellular matrices in glioma cells. Nestin may serve as a novel candidate for molecular-targeted therapy for gliomas, including glioblastomas.
  • Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods, Yoko Matsuda, Yoko Kawamoto, Kiyoshi Teduka, Wei-Xia Peng, Tetsushi Yamamoto, Toshiyuki Ishiwata, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 38(5), 1253 - 1258, May 2011 , Refereed
    Summary:Cell culture is one of the most important methods of research :in molecular and cellular biology, and various culture systems have been developed, including two-dimensional (2D), three-dimensional (3D) and floating culture systems. In the present study, we examined morphological changes and different expression patterns of cytoskeletal proteins in three different types of nervous system tumor cells grown in 2D, 3D and floating cell cultures. A172, KG-1-C and IMR-32 cells showed marked morphological changes, depending on the cell culture methods. F-actin expression was clearly observed at the level of the cells nearest the plate surface in 2D and 313 cultures. On the other hand, expression of F-actin was weak in the floating culture system. a-tubulin was detected in the cytoplasm of cells in 2D culture, but in floating and 3D cultures, a-tubulin was expressed in the peripheral regions of spheres and spheroids. In conclusion, this study demonstrated that nervous system tumor cells showed different alterations in morphology, and different cytoskeletal protein expression patterns, depending on the culture methods.
  • Nestin regulates pancreatic cancer stem cell functions, Yoko Matsuda, Kazuya Yamahatsu, Kiyoko Kawahara, Taeko Suzuki, Takenori Fujii, Tetsushi Yamamoto, Murray Korc, Zenya Naito, Toshiyuki Ishiwata, CANCER RESEARCH, CANCER RESEARCH, 71, Apr. 2011 , Refereed
  • Nestin as a novel angiogenic marker and target for anti-angiogenic therapy in human pancreatic cancer, Kazuya Yamahatsu, Yoko Matsuda, Tetsushi Yamamoto, Takayuki Aimoto, Yoshiharu Nakamura, Makoto Hiroi, Eiji Uchida, Zenya Naito, Toshiyuki Ishiwata, CANCER RESEARCH, CANCER RESEARCH, 71, Apr. 2011 , Refereed
  • Fibroblast growth factor receptor 2 as a novel therapeutic target for cancer cell growth and angiogenesis in human pancreatic cancer, Toshiyuki Ishiwata, Yoko Matsuda, Kiyoko Kawahara, Tetsushi Yamamoto, Kiyoshi Teduka, Taeko Suzuki, Wei-Xia Peng, Murray Korc, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 71, Apr. 2011 , Refereed
  • The stem cell marker nestin is a novel therapeutic target to suppress tumor growth and invasion of malignant melanoma, Michiko Akiyama, Kiyoko Kawahara, Yoko Matsuda, Taeko Suzuki, Ryoko Takayama, Tetsushi Yamamoto, Toshiyuki Ishiwata, Seiji Kawana, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 71, Apr. 2011 , Refereed
  • The stem cell marker nestin regulates growth, invasion and stemness of malignant melanomas, Michiko Akiyama, Kiyoko Kawahara, Yoko Matsuda, Ryoko Takayama, Tetsushi Yamamoto, Toshiyuki Ishiwata, Seiji Kawana, Zenya Naito, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 28, S14 - S14, 2011 , Refereed
  • Immunohistochemical analysis of cancer stem cell markers in human pancreatic cancer tissues, Tetsushi Yamamoto, Yoko Matsuda, Yoko Kawamoto, Kiyoko Kawahara, Zenya Naito, Toshiyuki Ishiwata, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 28, S15 - S15, 2011 , Refereed
  • Expression and roles of nestin in human uterine cervical intraepithelial neoplasia and cervical cancer, Atsuki Sato, Toshiyuki Ishiwata, Kiyoko Kawahara, Tetsushi Yamamoto, Yoko Matsuda, Hirobumi Asakura, Toshiyuki Takeshita, Zenya Naito, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 28, S15 - S15, 2011 , Refereed
  • Enhanced expression of lumican inhibited the attachment and growth of human embryonic kidney 293 cells, Toshiyuki Ishiwata, Tetsushi Yamamoto, Kiyoko Kawahara, Yoko Kawamoto, Yoko Matsuda, Shunji Ishiwata, Zenya Naito, EXPERIMENTAL AND MOLECULAR PATHOLOGY, EXPERIMENTAL AND MOLECULAR PATHOLOGY, 88(3), 363 - 370, Jun. 2010 , Refereed
    Summary:Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression was reported in various kinds of tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in pancreatic cancer correlated with an advanced stage and retroperitoneal and duodenal invasion. In this study, we clarified whether the enhanced expression of lumican contributes to cellular attachment, growth, colony formation, migration and invasion. HEK 293 cell, stably transfected with lumican cDNA synthesized and secreted a 50 kDa lumican protein at high levels in culture medium. The cells showed a polygonal appearance with long projections and the degree of adhesion of the cells to fibronectin was lower than that of empty vector transfected control cells (mock cells). In contrast, the degree of adhesion of the cells to type I collagen was not different from that of mock cells. The expression levels of alpha 5 integrin, the major integrin subunit for fibronectin, were lower in lumican-transfected HEK cells than in mock cells. Furthermore, lumican-transfected HEK cells showed reduced growth rates in vitro and did not form colonies in soft agar. Phosphorylation of AKT, extracellular signal-regulated kinase (ERK) 1/2 and mammalian target of rapamycin (mTOR) decreased in the lumican-transfected HEK cells. Cell migration and invasion were not altered in lumican-transfected HEK cells and mock cells. These findings indicate that the 50 kDa lumican protein plays important roles in the inhibition of HEK cell attachment and growth, and it might inhibit the activation of integrin pathways. (C) 2010 Elsevier Inc. All rights reserved.
  • Fibroblast growth factor receptor (FGFR) 2 Illc, is a novel molecular target for suppressing pancreatic cancer cell growth and migration, Toshiyuki Ishiwata, Yoko Matsuda, Kiyoko Kawahara, Tetsushi Yamamoto, Kiyoshi Teduka, Eiji Uchida, Murray Korc, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 70, Apr. 2010 , Refereed
  • Fibroblast growth factor receptor 2IIIc enhances the growth of colorectal adenocarcinoma cells, Zenya Naito, Yoko Matsuda, Yoko Kawamoto, Takenori Fujii, Taeko Suzuki, Munehiko Onda, Tetsushi Yamamoto, Tomoko Seya, Yoshiharu Ohaki, Toshiyuki Ishiwata, CANCER RESEARCH, CANCER RESEARCH, 70, Apr. 2010 , Refereed
  • Lumican, a Small Leucine-Rich Proteoglycan Family Member Has a Potential to Develop New Medicine for the Pancreatic Cancer Patients, Tetsushi Yamamoto, Toshiyuki Ishiwata, Yoko Matsuda, Munehiko Onda, Yuri Ono, Kiyoko Kawahara, Takenori Fujii, Yoko Kawamoto, Eiji Uchida, Zenya Naito, GASTROENTEROLOGY, GASTROENTEROLOGY, 136(5), A422 - A423, May 2009 , Refereed
  • Down-regulation of Nestin inhibited the invasion and migration of pancreatic cancer cells, Toshiyuki Ishiwata, Yoko Matsuda, Tetsushi Yamamoto, Masao Kawamoto, Kiyoko Kawahara, Kiyoshi Teduka, Takenori Fujii, Nando Nakazawa, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 69, May 2009 , Refereed
  • Fibroblast growth factor receptor 2 IIIc and development of colorectal adenocarcinoma, Yoko Matsuda, Munehiko Onda, Tetsushi Yamamoto, Yoko Kawamoto, Taeko Suzuki, Nando Nakazawa, Tomoko Seya, Yoshiharu Ohaki, Toshiyuki Ishiwata, Zenya Naito, CANCER RESEARCH, CANCER RESEARCH, 69, May 2009 , Refereed
  • Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells, Kosuke Narita, Takenori Fujii, Toshiyuki Ishiwata, Tetsushi Yamamoto, Yoko Kawamoto, Kiyoko Kawahara, Nando Nakazawa, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 34(2), 355 - 360, Feb. 2009 , Refereed
    Summary:Keratinocyte growth factor (KGF), which is also called fibroblast growth factor (FGF)-7, belongs to the FGF family. KGF is not commonly produced by human cancer cells, but the KGF receptor (KGFR) is expressed in most cancer cells and particularly highly expressed in well-differentiated types of cancer. Recently, it has been reported that vascular endothelial growth factor (VEGF)-A expression is induced by KGF in pancreatic cancer cells. VEGF-A is produced by some cancer cells and plays important roles in the angiogenesis and metastasis of cancer cells including those in the colorectum. In this study, we examined whether recombinant human KGF (rhKGF) induces major angiogenic growth factors including VEGF-A, FGF-2 and hepatocyte growth factor (HGF) in human colorectal cancer cells (HCT-15), which express a high level of KGFR, but a low or negligible level of KGF. rhKGF significantly increased the VEGF-A expression level in a serum-free medium of HCT-15 cells, but FGF-2 and HGF expression levels were too low to detect. Furthermore, the expression levels of the angiogenic growth factors were evaluated in KGF-transfected HCT-15 cells, which were induced to stably overexpress KGF by KGF gene transfection and mock-transfected cells (Mock). KGF and VEGF-A expression levels in the cells and the protein concentrations in serum-free medium were significantly higher in KGF-transfected HCT-15 cells than in Mock cells. In contrast, the FGF-2 and HGF mRNA expression levels were not significantly different between KGF-transfected HCT-15 cells and Mock cells and the protein concentrations in scrum-free medium of the cells were below the detection level. These findings suggest that administration of rhKGF and over-expression of endogenous KGF genes in colorectal cancer cells increase VEGF-A production and may relate to angiogenesis in cancer.
  • Expression and roles of lumican in lung adenocarcinoma and squamous cell carcinoma, Yoko Matsuda, Tetsushi Yamamoto, Mitsuhiro Kudo, Kiyoko Kawahara, Masashi Kawamoto, Yuki Nakajima, Kiyoshi Koizumi, Nando Nakazawa, Toshiyuki Ishiwata, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 33(6), 1177 - 1185, Dec. 2008 , Refereed
    Summary:Lumican is a member of a small leucine-rich proteoglycan family and is highly expressed in several types of cancer cells and/or stromal tissue. Lumican expression in the cytoplasm in advanced colorectal cancer correlates with poor patient prognosis. The expression of lumican in stromal tissues is associated with a high tumor grade, a low estrogen receptor expression level, and Young age in breast cancer and is associated with tumor invasion and advanced stage in pancreatic cancer. In this Study, we examined the expression and role of lumican in lung cancer including adenocarcinoma (ADC) and squamous cell carcinoma (SqCC). Immunohistochemically, lumican was weakly expressed in vascular smooth muscle cells, perivascular and peribronchial connective tissues and bronchial epithelium of normal lung tissues. In lung cancer tissues, lumican was localized in the cytoplasm of cancer cells and/or stromal tissues adjacent to cancer cells. In ADC. the expression level of lumican in cancer cells correlated with pleural invasion and larger tumor size, but that of lumican in stromal tissues did not correlate with clinicopathological factors. In SqCC, the expression level of lumican in cancer cells con-elated with formation of a keratinized pattern, and stromal lumican expression correlated with vascular invasion. In SqCC and ADC, the expression level of lumican in cancer cells did not correlate with patient prognosis. In lung cancer cell lines, lumican mRNA and protein were expressed in LC-1/Sq and EBC-1 cells established from SqCC, and A549, RERF-LC-KJ and PC-3 cells from ADC. The molecular weight of lumican extracted from the cytoplasm of lung cancer cells differed from that in the culture medium owing to glycosylation of the protein. These findings suggest that the
  • Immunoprecipitation and MALDI-MS identification of lithocholic acid-tagged proteins in liver of bile duct-ligated rats, Shigeo Ikegawa, Tetsushi Yamamoto, Hiromi Ito, Shunji Ishiwata, Toshihiro Sakai, Kuniko Mitamura, Masako Maeda, JOURNAL OF LIPID RESEARCH, JOURNAL OF LIPID RESEARCH, 49(11), 2463 - 2473, Nov. 2008 , Refereed
    Summary:Formation of covalently bound protein adducts with lithocholic acid (LCA) might explain LCA's known carcinogenic properties and hepatotoxicity. We performed studies aimed at isolating and identifying hepatic proteins tagged with LCA, presumably via the epsilon-amino group of lysine residues. Antibodies recognizing the 3 alpha-hydroxy-5 beta-steroid moiety of LCA were generated by immunizing rabbits with immunogens in which the carboxyl group of LCA was coupled to BSA via a 6-aminohexanoic acid and/or succinic acid spacer. The resulting antibodies reacted with N-alpha (t-butoxycarbonyl)-L-lysine-epsilon-LCA, the amidated and nonamidated forms of LCA, as well as synthetically prepared LCA adducts with ovalbumin and lysozyme. Proteins tagged with LCA in the liver of bile duct-ligated rats were isolated by immunoprecipitation using these antibodies. Proteins were isolated by two-dimensional electrophoresis, and their structure was identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry and computer-assisted programs. Proteins labeled with LCA were Rab-3, Rab-12, Rab-16, and M-Ras. Rab proteins are Ras-like small GTP binding proteins that regulate vesicle trafficking pathways. The covalent binding of the Rab proteins with LCA may influence vesicular transport or binding of vesicles to their cognate membrane and may contribute to LCA-induced liver toxicity.
  • Production and Characterization of a Monoclonal Antibody to Capture Proteins Tagged with Lithocholic Acid, Shigeo Ikegawa, Tetsushi Yamamoto, Takahiro Miyashita, Rika Okihara, Shunji Ishmata, Toshihiro Sakai, Rung-Hwa Chong, Masako Maeda, Alan F. Hofmann, Kuniko Mitamura, ANALYTICAL SCIENCES, ANALYTICAL SCIENCES, 24(11), 1475 - 1480, Nov. 2008 , Refereed
    Summary:Reactive metabolic-modified proteins have been proposed to play an important role in the mechanism(s) of the hepatotoxicity and colon cancer of lithocholic acid (LCA). To identify cellular proteins chemically modified with LCA, we have generated a monoclonal antibody that recognizes the 3 alpha-hydroxy-5 beta-steroid moiety of LCA. The spleen cells from a BALB/c mouse, which was immunized with an immunogen in which the side chain of LCA was coupled to bovine serum albumin (BSA) via a succinic acid spacer, was fused with SP2/0 myeloma cells to generate antibody-secreting hybridoma clones. The resulting monoclonal antibody (gamma 2b, kappa) was specific to LCA-N-alpha-BOC-lysine as well as the amidated and nonamidated forms of LCA. The immunoblot enabled the detection of LCA residues anchored on BSA and lysozyme. The antibody will be useful for monitoring the generation, localization, and capture of proteins tagged with LCA, which may be the cause of LCA-induced toxicity.
  • Expression and roles of keratinocyte growth factor and its receptor in esophageal cancer cells, Masanori Yoshino, Toshiyuki Ishiwata, Masanori Watanabe, Tetsuro Matsunobu, Osamu Komine, Yuri Ono, Tetsushi Yamamoto, Takenori Fujii, Koshi Matsumoto, Akira Tokunaga, Zenya Naito, INTERNATIONAL JOURNAL OF ONCOLOGY, INTERNATIONAL JOURNAL OF ONCOLOGY, 31(4), 721 - 728, Oct. 2007 , Refereed
    Summary:The keratinocyte growth factor receptor (KGFR), also known as FGFR2 IIIb, is mainly localized in epithelial cells and is activated by the keratinocyte growth factor (KGF) that is predominantly synthesized by mesenchymal cells. In this study, we examined the roles of KGFR and KGF in human esophageal cancer (EC). In noncancerous esophageal tissues, KGFR was localized in epithelial cells from the basal region of the epithelium to the lower one-third of the epithelium, and KGF was weakly localized in the basal to parabasal epithelial cells. On the other hand, Ki-67 was localized in the parabasal cells. In EC tissues, KGFR and KGF were expressed in cancer cells in 22 and 37 of 54 patients, respectively. The coexpression of KGFR and KGF in cancer cells was detected in 14 of 54 (26%) patients. Clinicopathologically, KGFR expression correlated with the well-differentiated cell type of EC (p<0.001), and KGF expression correlated with lymphatic invasion and lymph node metastasis (p=0.004 and 0.021, respectively). The coexpression of KGFR and KGF in cancer cells correlated with the well-differentiated cell type of EC (p=0.001). KGFR-positive, KGF-positive and KGFR/KGF coexpression patients tended to have shorter survival rates, but the survival rates were not statistically significantly different (p=0.44, 0.059 and 0.112, respectively). In human EC cell lines (TE-1, TE-8 and TE-11), KGFR mRNA was expressed but no KGF mRNA was detected. The KGFR mRNA level was highest in TE-1 cells, derived from well-differentiated SCC and lowest in TE-8 cells. KGFR was detected in the cancer cell lines by Western blot analysis. Recombinant human KGF significantly stimulated the growth of TE-8 and -11 cells, derived from moderately differentiated SCC, but had no effect on TE-1 cell growth. These results suggest that KGFR expression correlates with the differentiation of a normal esophageal epithelium and the well-differentiated cell type of EC. On the other hand, KGF may induce the growth of some EC cells in a paracrine manner and closely correlates with lymphatic invasion and lymph node metastasis.
  • Role of lumican in cancer cells and adjacent stromal tissues in human pancreatic cancer, Toshiyuki Ishiwata, Kazumitsu Cho, Kiyoko Kawahara, Tetsushi Yamamoto, Yuri Fujiwara, Eiji Uchida, Takashi Tajiri, Zenya Naito, ONCOLOGY REPORTS, ONCOLOGY REPORTS, 18(3), 537 - 543, Sep. 2007 , Refereed
    Summary:Lumican is a member of a small leucine-rich proteoglycan family and its overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine cell, uterine cervical and pancreatic cancers. The expression of lumican in stromal tissues in breast cancer is associated with a high tumor grade, a low estrogen receptor expression level and young age. Lumican expression in the cytoplasm in advanced colorectal cancer is correlated with a poor prognosis. Lumican expression was previously reported in pancreatic cancer, but the role of lumican in pancreatic cancer is still not well understood. In this study, we aimed to clarify the role of lumican in pancreatic cancer. Reverse-tran scription polymerase chain reaction and Western blot analyses revealed lumican mRNA and protein expression in six pancreatic ductal adenocarcinoma cell lines (i.e. PANC-1, MIA PaCa-2, KLM-1, Capan-1, PK-1 and PK-8). On the basis of its immunoreactivity, lumican was found to be localized in islet cells of normal pancreatic tissues, but not in exocrine cells. In pancreatic cancer tissues, lumican was predominantly localized in the cytoplasm of cancer cells in 30 out of 53 (56.6%) cancer patients, whereas lumican was detected in stromal tissues in 36 out of 53 (67.9%) cancer patients. Lumican expression in pancreatic cancer cells did not correlate with clinicopathological factors, whereas lumican expression in stromal tissues correlated with the female gender, advanced stage, retroperitoneal and duodenal invasion and residual tumor (p=0.030, 0.038, 0.049, 0.049 and 0.048, respectively). Patients with lumican-positive cancer cells tended to survive longer than those with lumican-negative cancer cells (p=0.286), but patients with lumican-positive stromal tissues had shorter survival than those with lumican-negative stromal tissues (p=0.062). These results suggest that lumican in stromal tissues plays an important role in the growth and invasion of pancreatic cancer.

Misc

  • メープルシロップ中タンパク質成分は新規大腸癌治療薬開発の標的となり得る(Protein components of maple syrup as a potential source to develop novel anti-cancer drugs for colorectal cancer), 山本 哲志, 森山 由瑛, 三田村 邦子, 多賀 淳, 日本癌学会総会記事, 77回, 2200, 2200,   2018 09
  • LC‐MS/MSを用いたTCA回路代謝物の一斉分析, 佐藤完太, 山口真史, 山本哲志, 三田村邦子, 多賀淳, JSBMS Letters, 43, Supplement, 139,   2018 08 25 , http://jglobal.jst.go.jp/public/201802251404128818
  • ホルマリン固定パラフィン包埋大腸癌組織を用いた新規大腸癌診断マーカーの探索とその定量法の開発, 山本哲志, 工藤光洋, 橋本知樹, 三田村邦子, 内藤善哉, 多賀淳, JSBMS Letters, 43, Supplement, 66,   2018 08 25 , http://jglobal.jst.go.jp/public/201802275120149317
  • クロマグロ抽出コラーゲンの悪性黒色腫細胞増殖への影響の検討, 久保田千晶, 山本哲志, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 138th, ROMBUNNO.27PA‐am281,   2018 , http://jglobal.jst.go.jp/public/201802224263184420
  • ショットガンプロテオミクス解析を用いた糖尿病白内障要因の解析, 大竹裕子, 山本哲志, 三田村邦子, 多賀淳, 長井紀章, 日本薬学会年会要旨集(CD-ROM), 138th, ROMBUNNO.26PA‐pm325,   2018 , http://jglobal.jst.go.jp/public/201802224797816915
  • プロテオーム解析によるすっぽんコラーゲンのヒト皮膚角化細胞への影響の検討, 山本哲志, 中西紗緒理, 三田村邦子, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 138th, ROMBUNNO.27PA‐am384,   2018 , http://jglobal.jst.go.jp/public/201802225198127089
  • 各種温度下におけるシクロホスファミドの最大ガス化量, 遠藤晋吾, 遠藤晋吾, 石渡俊二, 山本理恵, 北野里佳, 藤本美弥, 山本哲志, 多賀淳, 升永早紀, 益子高, 福田由之, 安井友佳子, 石坂敏彦, 井上知美, 小竹武, 日本薬学会年会要旨集(CD-ROM), 138th, ROMBUNNO.28PA‐am347S,   2018 , http://jglobal.jst.go.jp/public/201802281009171344
  • 細胞外基質lumicanの膵臓癌特異糖鎖修飾を標的とした新規治療法の開発, 山本哲志, 多賀淳, 三田村邦子, 大和証券ヘルス財団研究業績集, 40, 93‐96,   2017 03 10 , http://jglobal.jst.go.jp/public/201702263534525132
  • 細胞外基質Lumicanの発現抑制による新規膵臓癌細胞増殖抑制法の開発, 山本哲志, 谷田和香奈, 橋本知樹, 三田村邦子, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 137th, ROMBUNNO.25PB‐am180,   2017 , http://jglobal.jst.go.jp/public/201702237981945675
  • 保湿化粧品材料コラーゲンの物理的保水能検証法, 久保田千晶, 北村真巳, 山本哲志, 松川泰治, 土井聡, 鹿島康浩, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 137th, ROMBUNNO.26Q‐pm15S,   2017 , http://jglobal.jst.go.jp/public/201702261688092599
  • 新規大腸癌治療薬開発のためのメープルシロップ中抗腫瘍成分の同定, 山本 哲志, 澁路 龍大, 三田村 邦子, 多賀 淳, 日本癌学会総会記事, 75回, P, 1276,   2016 10
  • 大腸がん細胞の細胞増殖におけるメープルシロップの役割について, 久保田 千晶, 山本 哲志, 三田村 邦子, 多賀 淳, 日本癌学会総会記事, 75回, P, 1277,   2016 10
  • ショットガンプロテオミクス解析に基づく,大腸癌における新規治療標的CyclophilinAの役割。, 高倉英樹, 山本哲志, 三田村邦子, 工藤光洋, 内藤善哉, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 136th, 4, ROMBUNNO.28O-PM03S, 73,   2016 03 , http://jglobal.jst.go.jp/public/201602274150482142
  • メープルシロップ投与による大腸癌細胞増殖抑制機構の検討, 山本哲志, 三田村邦子, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 136th, 4, ROMBUNNO.27AB‐PM272, 204,   2016 03 , http://jglobal.jst.go.jp/public/201602281216293857
  • スッポンからのコラーゲン抽出法の最適化, 久保田千晶, 山本哲志, 上村健太郎, 澤岻有喜, 三田村邦子, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 136th, 2, ROMBUNNO.28R-AM07, 60,   2016 03 , http://jglobal.jst.go.jp/public/201602283376938431
  • LC/ESI‐MSによる乾燥ろ紙尿中糖質コルチコイド代謝物スクリーニング法の開発, 三田村邦子, 森莉子, 上田麻美子, 亀井美希, 山本哲志, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 136th, 2, ROMBUNNO.28AB‐AM342, 265,   2016 03 , http://jglobal.jst.go.jp/public/201602291196662880
  • 細胞外基質lumicanの膵臓癌特異糖鎖修飾を標的とした新規治療法の開発, 山本哲志, 日本膵臓病研究財団研究報告書, 22nd, 119, 123,   2015 12 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201602207719857761
  • 革新的創薬開発と実用化 天然甘味料メープルシロップの大腸癌治療への応用, 山本哲志, 化学工業, 66, 12, 919, 924,   2015 12 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502203431738820
  • Application of Therapeutic Method for Colorectal Cancer Using Natural Sweetener Maple Syrup, 山本 哲志, 化學工業, 66, 12, 919, 924,   2015 12 , http://ci.nii.ac.jp/naid/40020659132
  • ホルマリン固定パラフィン包埋大腸癌組織を用いたプロテオーム解析によるバイオマーカーの探索, 山本 哲志, 工藤 光洋, 彭 為霞, 高田 英志, 三田村 邦子, 多賀 淳, 内藤 善哉, 日本癌学会総会記事, 74回, P, 1358,   2015 10
  • 大腸癌細胞の増殖・浸潤におけるメープルシロップの効果, 久保田 千晶, 山本 哲志, 三田村 邦子, 多賀 淳, 日本癌学会総会記事, 74回, P, 1308,   2015 10
  • メープルシロップを用いた大腸癌抗腫瘍効果の検討, YAMAMOTO TETSUSHI, UEMURA KENTARO, MITAMURA KUNIKO, TAGA ATSUSHI, 日本薬学会年会要旨集(CD-ROM), 135th, ROMBUNNO.27H-PM14,   2015 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502285760476858
  • LC/ESI‐MS/MSによる尿中抱合型テトラヒドロコルチコステロイドのプロファイル分析, MITAMURA KUNIKO, UEDA MAMIKO, YAMAMOTO TETSUSHI, TAGA ATSUSHI, IKEGAWA SHIGEO, 日本薬学会年会要旨集(CD-ROM), 135th, ROMBUNNO.27PA-PM087,   2015 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502295108504314
  • ホルマリン固定パラフィン包埋大腸癌組織を用いた新規治療標的の探索(Proteomic analysis of formalin-fixed paraffin-embedded colorectal cancer tissue for identification of therapeutic target), 山本 哲志, 工藤 光洋, 彭 為霞, 高田 英志, 三田村 邦子, 多賀 淳, 内藤 善哉, 日本癌学会総会記事, 73回, P, 3254,   2014 09
  • FFPE切片を用いたケラトアカントーマのプロテオーム解析(Proteome analysis using formalin-fixed paraffin-embedded keratoacanthoma tissue), 神崎 亜希子, 工藤 光洋, 安齋 眞一, 山本 哲志, 藤井 雄文, 手塚 潔, 彭 為霞, 和田 龍一, 佐伯 秀久, 内藤 善也, 日本癌学会総会記事, 73回, P, 3338,   2014 09
  • 肝細胞癌のホルマリン固定パラフィン包埋組織ブロックを用いた発現低下を示すタンパク質の検討(Identification of down-regulated proteins using formalin-fixed paraffin-embedded HCC tissue), 高田 英志, 工藤 光洋, 山本 哲志, 和田 龍一, 彭 為霞, 石野 孔祐, 手塚 潔, 藤井 雄文, 河本 陽子, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 73回, P, 3339,   2014 09
  • Maple syrupの大腸癌抑制作用(Anti-cancer Effect of Maple Syrup for Colorectal Cancer), 上村 健太朗, 山本 哲志, 三田村 邦子, 多賀 淳, 日本癌学会総会記事, 73回, P, 2416,   2014 09
  • LC/ESI‐LIT‐MS/MSにおけるPQD及びCID測定による尿中硫酸抱合型ステロイドの分析, 三田村邦子, 蔵渕慧, 上田麻美子, 池川繁男, 山本哲志, 多賀淳, JSBMS Lett, 39, Supplement, 62,   2014 09 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402294671380938
  • 卵巣癌FFPE組織を用いたプロテオーム解析による新規バイオマーカーの探索, 高屋茜, 彭為霞, 山本哲志, 石野孔祐, 工藤光洋, 竹下俊行, 内藤善哉, 日本婦人科腫よう学会雑誌, 32, 3, 517,   2014 06 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402257010086623
  • FFPE組織を用いた卵巣癌プロテオーム解析による新規バイオマーカーの探索, 高屋茜, 彭為霞, 紺野亜希子, 高田英志, 藤井雄文, 手塚潔, 石野孔祐, 山本哲志, 工藤光洋, 内藤善哉, 日本病理学会会誌, 103, 1, 372,   2014 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402264829235381
  • 肝細胞癌において発現低下を示す代謝関連タンパク質の検討, 高田英志, 工藤光洋, 山本哲志, 彭為霞, 藤井雄文, 手塚潔, 石野孔祐, 高屋茜, 紺野亜希子, 川原清子, 河本陽子, 内田英二, 内藤善哉, 日本病理学会会誌, 103, 1, 317,   2014 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402291679702048
  • ショットガンプロテオミクス解析による大腸癌早期発見のための新規診断マーカーの探索, 上村健太朗, 山本哲志, 三田村邦子, 工藤光洋, 内藤善哉, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 134th, ROMBUNNO.30PML-139S,   2014 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402203597392796
  • メープルシュガーにおける血糖値上昇抑制効果, 多賀淳, 長井紀章, 山本哲志, 三田村邦子, 伊藤吉將, 日本薬学会年会要旨集(CD-ROM), 134th, ROMBUNNO.28AML-101,   2014 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402251488298822
  • プロテオーム解析を用いたlumicanの膵臓癌細胞増殖制御機構の解析, 山本哲志, 木場克斗, 三田村邦子, 工藤光洋, 内藤善哉, 多賀淳, 日本薬学会年会要旨集(CD-ROM), 134th, ROMBUNNO.30PML-136,   2014 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402282507468940
  • LC/ESI‐MS/MSによるラット胆汁中抱合型フェニル酢酸の同定, 三田村邦子, 大橋勇斗, 山本哲志, 多賀淳, 池川繁男, 日本薬学会年会要旨集(CD-ROM), 134th, ROMBUNNO.29AML-070,   2014 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402292968391588
  • 肝細胞癌ホルマリン固定パラフィン包埋組織を用いたプロテオーム解析による新規バイオマーカーの探索(Proteomic analysis for identification of biomarkers using formalin-fixed paraffin-embedded HCC tissue), 高田 英志, 山本 哲志, 工藤 光洋, 彭 為霞, 手塚 潔, 藤井 雄文, 上田 純志, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 72回, 447, 447,   2013 10
  • 上皮性卵巣癌のホルマリン固定パラフィン包埋組織からの蛋白質プロテオーム解析(Proteome analysis of formalin-fixed paraffin-embedded epithelial ovarian cancer tissues), 高屋 茜, 彭 為霞, 紺野 亜希子, 高田 英志, 手塚 潔, 藤井 雄文, 山本 哲志, 工藤 光洋, 竹下 俊行, 内藤 善哉, 日本癌学会総会記事, 72回, 447, 447,   2013 10
  • ホルマリン固定パラフィン包埋肝細胞癌組織を用いたプロテオーム解析(Proteome analysis using formalin-fixed paraffin-embedded hepatocellular carcinoma tissues), 工藤 光洋, 高田 英志, 彭 為霞, 山本 哲志, 藤井 雄文, 手塚 潔, 内藤 善哉, 日本癌学会総会記事, 72回, 447, 448,   2013 10
  • 子宮類内膜腺癌及び前癌病変におけるFGFR2IIIcの役割解明, 彭為霞, 工藤光洋, 山本哲志, 藤井雄文, 手塚潔, 内藤善哉, 日本病理学会会誌, 102, 1, 374,   2013 04 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302221765349479
  • 卵巣癌における膜結合型ムチン及びHer2の免疫組織化学的検討, 高屋茜, 彭為霞, 紺野亜希子, 高田英志, 藤井雄文, 手塚潔, 山本哲志, 工藤光洋, 内藤善哉, 日本病理学会会誌, 102, 1, 382,   2013 04 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302239348591212
  • 肝細胞癌にてダウンレギュレートしたタンパク質のホルマリン固定パラフィン包埋組織からのプロテオーム解析, 工藤光洋, 山本哲志, 高田英志, 彭為霞, 藤井雄文, 手塚潔, 内藤善哉, 日本病理学会会誌, 102, 1, 465,   2013 04 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302265991919625
  • ケラトアカントーマのホルマリン固定パラフィン包埋組織によるプロテオーム解析, 紺野亜希子, 工藤光洋, 山本哲志, 安齋眞一, 藤井雄文, 手塚潔, 彭為霞, 川名誠司, 内藤善哉, 日本病理学会会誌, 102, 1, 479,   2013 04 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302266812725089
  • ホルマリン固定パラフィン包埋組織ブロックを用いた肝細胞癌のプロテオミクス解析, 高田英志, 山本哲志, 工藤光洋, 彭為霞, 藤井雄文, 手塚潔, 内田英二, 内藤善哉, 日本病理学会会誌, 102, 1, 465,   2013 04 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302298501935925
  • 肝細胞癌のホルマリン固定パラフィン包埋組織ブロックを用いたプロテオミクスによるバイオマーカーの探索, 高田英志, 山本哲志, 谷合信彦, 真々田裕宏, 吉岡正人, 川野陽一, 水口義昭, 清水哲也, 上田純志, 内藤善哉, 内田英二, 日本外科学会雑誌, 114, 646,   2013 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302285296414882
  • 大腸癌ホルマリン固定パラフィン包埋組織を用いたプロテオーム解析による新規診断マーカーの探索, 山本哲志, 工藤光洋, 高田英志, 彭為霞, 手塚潔, 藤井雄文, 内藤善哉, 日本薬学会年会要旨集(CD-ROM), 133rd, ROMBUNNO.29Q-PM26,   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302226684546050
  • 悪性黒色腫の増殖、浸潤、転移におけるネスチンの役割の検討(Nestin Plays Important Roles in Cell Growth, Invasion and the Metastasis of Malignant Melanoma), 秋山 美知子, 松田 陽子, 萩尾 真人, 川原 清子, 鈴木 妙子, 山本 哲志, 川名 誠司, 石渡 俊行, 内藤 善哉, 日本癌学会総会記事, 71回, 423, 424,   2012 08
  • Bowen病と日光角化症における病理組織学的鑑別診断マーカーとしてのlumicanの発現の検討, 高山 良子, 安齋 眞一, 石渡 俊行, 山本 哲志, 松田 陽子, 内藤 善哉, 川名 誠司, 日本皮膚悪性腫瘍学会学術大会プログラム・抄録集, 28回, 165, 165,   2012 06
  • Seborrheic keratosis,clonal typeとhidroacanthoma simplexにおけるlumicanの発現の検討, 高山良子, 安齋眞一, 石渡俊行, 山本哲志, 松田陽子, 内藤善哉, 川名誠司, 日本皮膚科学会雑誌, 122, 4, 1222,   2012 04 15 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202202362491982
  • A case of nodular fasciitis in parotid gland region, PENG Wei‐Xia, KUDO Mitsuhiro, YAMAMOTO Tetsushi, INAI Shunta, FUJII Takenori, TEDUKA Kiyoshi, KAWAHARA Kiyoko, MATSUDA Yoko, ISHIWATA Toshiyuki, NAITO Zenya, 日本病理学会会誌, 101, 1, 331,   2012 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202269789417276
  • 子宮頸部異形成と子宮頸癌におけるnestinの発現とCancer stem cellとの関連, 佐藤杏月, 石渡俊行, 山本哲志, 松田陽子, 朝倉啓文, 竹下俊行, 内藤善哉, 日本病理学会会誌, 101, 1, 277,   2012 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202210728139384
  • 膵癌細胞における分泌型lumicanの増殖・浸潤への関与, 山本哲志, 工藤光洋, 川原清子, 彭為霞, 松田陽子, 石渡俊行, 内藤善哉, 日本病理学会会誌, 101, 1, 370,   2012 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202217069698316
  • 皮膚腫瘍の病理組織診断におけるlumicanの有用性の検討, 高山良子, 石渡俊行, 安齋眞一, 山本哲志, 松田陽子, 川名誠司, 内藤善哉, 日本病理学会会誌, 101, 1, 248,   2012 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202218372122245
  • 膵癌・PanINにおける癌幹細胞マーカーの発現, 呉壮香, 松田陽子, 萩尾真人, 鈴木妙子, 柳澤祐次, 上田純志, 山初和也, 山本哲志, 石渡俊行, 内藤善哉, 日本病理学会会誌, 101, 1, 369,   2012 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202241088019452
  • Nestinによる膵癌腫瘍新生血管増殖制御と新たな分子標的治療の可能性, 山初和也, 松田陽子, 石渡俊行, 山本哲志, 萩尾真人, 相本隆幸, 中村慶春, 松下晃, 上田純志, 内田英二, 内藤善哉, 日本外科学会雑誌, 113, 2, 198, 198,   2012 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202203896735794
  • 膵臓癌細胞の増殖・浸潤の制御に関わるlumicanの機能解析, 山本哲志, 工藤光洋, 彭為霞, 川原清子, 手塚潔, 藤井雄文, 松田陽子, 石渡俊行, 内藤善哉, 日本薬学会年会要旨集, 132nd, 4, 176,   2012 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202226102386413
  • 癌分子標的治療up to date Nestinによる膵癌腫瘍新生血管増殖制御と新たな分子標的治療の可能性, 山初 和也, 松田 陽子, 石渡 俊行, 山本 哲志, 萩尾 真人, 相本 隆幸, 中村 慶春, 松下 晃, 上田 純志, 内田 英二, 内藤 善哉, 日本外科学会雑誌, 113, 臨増2, 198, 198,   2012 03
  • 子宮内膜癌及び前癌病変におけるFGFR2IIIcの役割の解明と新規治療及び予防を標的とした有効性の研究, 彭為霞, 内藤善哉, 石渡俊行, 松田陽子, 山本哲志, 大和証券ヘルス財団研究業績集, 35, 35, 48, 52,   2012 03 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202281648873022
    Summary:著者らが切除した子宮類内膜腺癌32例(年齢32〜84歳、中間年齢57歳)を対象に、免疫組織化学染色法を用いてFGFR2IIIcの発現と局在性について検討した。その結果、正常子宮内膜においてFGFR2IIIcは全例陽性を示したが、いずれも軽度の発現であった。一方、異型増殖症やGrade 1の類内膜腺癌においてはFGFR2IIIcの過剰発現が認められた。また、類内膜腺癌ではGrade 1に比しGrade 2、3と分化度の低下した癌で、FGFR2IIIcの発現量が有意に減少することが明らかとなった。
  • ヒト膵癌組織における各種癌幹細胞マーカー発現の検討, 山本哲志, 松田陽子, 萩尾真人, 河本陽子, 川原清子, 内藤善哉, 石渡俊行, 日本病理学会会誌, 100, 2, 30,   2011 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102289668416551
  • 膵癌の新規分子標的としての線維芽細胞増殖因子受容体2(FGFR2)(Fibroblast growth factor receptor 2 as a novel molecular target for human pancreatic cancer), 石渡 俊行, 松田 陽子, 上田 純志, 川原 清子, 萩尾 真人, 山本 哲志, 内田 英二, コーク・マレー, 内藤 善哉, 日本癌学会総会記事, 70回, 68, 68,   2011 09
  • 皮膚扁平上皮腫瘍性疾患におけるlumicanの発現(Expression of lumican in in situ and invasive lesions of squamous cell carcinoma of the skin), 高山 良子, 石渡 俊行, 安齋 眞一, 山本 哲志, 松田 陽子, 川名 誠司, 内藤 善哉, 日本癌学会総会記事, 70回, 200, 200,   2011 09
  • 膵癌血管内皮細胞におけるNestinの発現と役割(Expression and Roles of Nestin in Vascular Endothelial Cells in Pancreatic Cancer), 山初 和也, 松田 陽子, 石渡 俊行, 山本 哲志, 萩尾 真人, 相本 隆幸, 中村 慶春, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 70回, 365, 366,   2011 09
  • The alterations in neurotransmitters and their metabolites in discrete brain regions in the rats after inhalation of disinfectant, glutaraldehyde or ortho-phthalaldehyde for 4 weeks, Hiroshi Katagiri, Tetsushi Yamamoto, Ayako Uchimura, Masashi Tsunoda, Yoshiharu Aizawa, Hiroshi Yamauchi, Industrial Health, 49, 328, 337,   2011 06 23 , 10.2486/indhealth.MS1156, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959290391&origin=inward
    Summary:Glutaraldehyde (GA) and ortho-phtalaldehyde (OPA) have been widely used as major components of disinfectants in hospitals. We evaluated the alterations in GA or OPA in rats after subacute inhalation exposure by determining levels of neurotransmitters (norepinephrine [NE], dopamine [DA], DA metabolites, dihydroxyphenylacetic acid [DOPAC] and homovanillic acid [HVA], indoleamine serotonin [5-HT] and 5-HT metabolite, 5-hydroxyindoleacetic acid [5-HIAA]) in discrete brain regions using high performance liquid chromatography (HPLC) equipped with an electrochemical detector. Female Wistar rats were exposed to 0, 50, 100, or 200 ppb gaseous GA or OPA by inhalation for 1 h per day, 5 d per week for 4 wk. Following the exposure, the brain of each rat was removed and dissected into cerebrum, cerebellum, medulla oblongata, midbrain, corpus striatum and hypothalamus. The neurotransmitters and their metabolites were extracted from each brain region, and determined by HPLC. Regarding GA, the daily water intake of the 50 or the 200 ppb exposed groups was significantly lower than that of the control. DA and 5-HIAA levels in the medulla oblongata among the GA exposed groups were significantly lower than those of the control. For OPA, the mean final body weight and daily food intake of the 100 or 200 ppb exposed groups were significantly lower than those of the control. The mean DA concentrations in the cerebrum in the groups exposed to OPA were significantly lower than those of the control. OPA may modulate DA metabolism in the cerebrum of female rats. The levels GA or OPA that induced alienations in neurotransmitters were comparable to those levels usually found in hospitals, further studies are warranted to evaluate the of safety of disinfectants containing GA or OPA. © 2011 by National Institute of Occupational Safety and Health.
  • 膵癌細胞の3次元スフェロイド培養における形態構造と細胞骨格の変化, 松田 陽子, 石渡 俊行, 河本 陽子, 川原 清子, 彭 為霞, 山本 哲志, 内藤 善哉, 未病と抗老化, 20, 87, 94,   2011 06
    Summary:3次元(3D)細胞培養は生体内環境を模倣することができる培養法として注目されている。今回我々は、NanoCulture plateを用いて、2次元(2D)培養と3D培養を比較し、ヒト膵癌細胞の形態と細胞骨格タンパクの発現の変化を検討した。検討したすべての膵癌細胞は2D培養で単層のシート状に増殖した。PANC-1とKLM-1細胞は3D培養でスフェロイドを形成したが、PK-45HとMIAPaCa-2細胞はスフェロイドを形成しなかった。3D培養では、細胞のプレートとの接着面に、F-actinの強い発現が認められ、細胞突起の形成が見られた。PANC-1細胞ではF-actinがNanoCulture plateのグリッド上に認められたが、PK-45Hでは認められなかった。細胞内のtubulinの発現は2D培養より3D培養の方が亢進していた。PANC-1とKLM-1のE-cadherin mRNAの発現レベルは、PK-45HとMIAPaCa-2よりも高値を示した。以上より、3D培養において、膵癌細胞がスフェロイドを形成し、形態学的変化と細胞骨格タンパクの発現変化が見られた。E-cadherinは膵癌細胞のスフェロイド形成に関係している重要な分子の1つであることが示唆された。3Dスフェロイド培養法は位相差顕微鏡や共焦点レーザー顕微鏡での細胞イメージングのために有用な方法であると考えられた。(著者抄録)
  • 膵癌における各腫瘍血管マーカーと臨床病理学的因子との関連, 山初和也, 松田陽子, 石渡俊行, 山本哲志, 河本陽子, 手塚潔, 相本隆幸, 中村慶春, 廣井信, 上田純志, 内田英二, 内藤善哉, 日本外科学会雑誌, 112, 686,   2011 05 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102278554418773
  • 神経膠芽腫の腫瘍幹細胞の維持にnestinが関わる可能性, 手塚潔, 石渡俊行, 河本陽子, 山本哲志, 松田陽子, 内藤善哉, 日本病理学会会誌, 100, 1, 454,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102204179488587
  • 子宮頸部異形成および子宮頸癌におけるNestinの発現, 佐藤杏月, 石渡俊行, 山本哲志, 河本陽子, 彭為霞, 松田陽子, 恩田宗彦, 朝倉啓文, 竹下俊行, 内藤善哉, 日本病理学会会誌, 100, 1, 459,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102214792268633
  • 振り子状の形態を呈した肝臓血管筋脂肪腫の1例, 梶尾円香, 彭為霞, 井内亜美, 松田陽子, 石渡俊行, 恩田宗彦, 山本哲志, 新井悟, 大秋美治, 内藤善哉, 日本病理学会会誌, 100, 1, 498,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102221093507718
  • 幹細胞マーカーのnestinによる悪性黒色腫の増殖と浸潤阻害, 秋山美知子, 松田陽子, 川原清子, 高山良子, 山本哲志, 石渡俊行, 川名誠司, 内藤善哉, 日本病理学会会誌, 100, 1, 313,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102234163292212
  • 膵癌特異分泌型lumicanの細胞増殖への関与, 山本哲志, 松田陽子, 川原清子, 彭為霞, 内藤善哉, 石渡俊行, 日本病理学会会誌, 100, 1, 319,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102240611773960
  • 線維芽細胞増殖因子受容体(FGFR2)を標的とした膵癌治療の研究, 石渡俊行, 松田陽子, 上田純志, 山本哲志, 彭為霞, 川原清子, 内田英二, MURRAY Korc, 内藤善哉, 日本病理学会会誌, 100, 1, 320,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102245632125570
  • 皮膚腫瘍組織におけるlumicanとそのmRNAの発現の検討, 高山良子, 石渡俊行, 安齋眞一, 山本哲志, 松田陽子, 藤井雄文, 鈴木妙子, 川名誠司, 内藤善也, 日本病理学会会誌, 100, 1, 358,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102252077293670
  • 膵癌培養細胞におけるnestinによるE‐cadherin制御機構の解明, 鈴木妙子, 松田陽子, 手塚潔, 山本哲志, 内藤善哉, 石渡俊行, 日本病理学会会誌, 100, 1, 347,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102257537291479
  • ヒト膵癌組織における,酵素抗体法を用いた膵癌幹細胞マーカーの検討, 櫻井星羅, 黒田諒, 石渡俊行, 松田陽子, 山本哲志, 河本陽子, 川原清子, 内藤善哉, 日本病理学会会誌, 100, 1, 497,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102261271025244
  • Nude mouseとNOG mouseを用いたヒト膵癌培養細胞転移モデルの比較検討, 山初和也, 松田陽子, 藤井雄文, 川原清子, 河本陽子, 手塚潔, 鈴木妙子, 山本哲志, 石渡俊行, 内藤善哉, 日本病理学会会誌, 100, 1, 455,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102261417758044
  • 星細胞腫と神経膠芽腫におけるCD44の発現と,抗CD44モノクローナル抗体の投与効果の検討, 吉田常恭, 手塚潔, 川原清子, 山本哲志, 松田陽子, 石渡俊行, 内藤善哉, 日本病理学会会誌, 100, 1, 494,   2011 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102279843740817
  • 安定過剰発現株を用いた膵臓癌におけるlumicanの機能解析, 山本哲志, 松田陽子, 彭為霞, 川原清子, 藤井雄文, 手塚潔, 内藤善哉, 石渡俊行, 日本薬学会年会要旨集, 131st, 4, 171,   2011 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102232568759751
  • 膵癌の浸潤・転移におけるNestinの役割の解明とNestinを標的とした膵癌治療の基礎的研究, 松田陽子, 内藤善哉, 石渡俊行, 山本哲志, 大和証券ヘルス財団研究業績集, 34, 40, 44,   2011 03 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102205703224661
  • 神経膠芽腫細胞におけるCD44の発現と,抗CD44モノクローナル抗体投与の効果, 吉田常恭, 手塚潔, 川原清子, 藤井雄文, 河本陽子, 鈴木妙子, 山本哲志, 松田陽子, 石渡俊行, 内藤善哉, 日本医科大学医学会雑誌, 6, 4, 214,   2010 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002257676438525
  • NESTIN'S INHIBITION OF THE MIGRATION, INVASION AND METASTASIS OF PANCREATIC CANCER CELLS, MATSUDA Yoko, ISHIWATA Toshiyuki, YAMAHATSU Kazuya, YAMAMOTO Tetsushi, SUZUKI Taeko, FUJII Takenori, KAWAHARA Kiyoko, KORC Murray, NAITO Zenya, 日本病理学会会誌, 99, 2, 39,   2010 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002230088190260
  • FIBROBLAST GROWTH FACTOR RECEPTOR 2 (FGFR2) AS A NOVEL THERAPEUTIC TARGET FOR PANCREATIC CANCER, ISHIWATA Toshiyuki, MATSUDA Yoko, KAWAHARA Kiyoko, YAMAMOTO Tetsushi, TEDUKA Kiyoshi, UCHIDA Eiji, KORC Murray, NAITO Zenya, 日本病理学会会誌, 99, 2, 39,   2010 09 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002245846337356
  • 神経膠芽腫細胞におけるNestinの発現とその役割(Expression and the roles of Nestin in glioblastoma cells), 手塚 潔, 石渡 俊行, 山本 哲志, 河本 陽子, 川原 清子, 松田 陽子, 内藤 善哉, 日本癌学会総会記事, 69回, 122, 122,   2010 08
  • 分泌型ルミカンによる膵臓癌細胞増殖への関与(Enhanced expression of secreted type of lumican induced pancreatic cancer cell growth), 山本 哲志, 石渡 俊行, 松田 陽子, 恩田 宗彦, 川原 清子, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 69回, 248, 249,   2010 08
  • 各種腫瘍血管マーカーを用いた膵癌腫瘍新生血管と臨床病理学的因子との関連(Identification of neovasculature using various endothelial cell markers in human pancreatic cancer), 山初 和也, 松田 陽子, 石渡 俊行, 山本 哲志, 河本 陽子, 手塚 潔, 相本 隆幸, 中村 慶春, 廣井 信, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 69回, 449, 449,   2010 08
  • ヒト膵癌培養細胞の三次元培養による細胞形態・細胞骨格タンパクの解析(Morphological and cytoskeletal analyses of human pancreatic cancer cells in novel three-dimensional spheroidal culture), 松田 陽子, 石渡 俊行, 山本 哲志, 河本 陽子, 藤井 雄文, 川原 清子, 内藤 善哉, 日本癌学会総会記事, 69回, 519, 519,   2010 08
  • Nestin発現制御による膵癌の浸潤,転移抑制機序の検討, 松田陽子, 石渡俊行, 山本哲志, 川原清子, 藤井雄文, 河本陽子, 手塚潔, 鈴木妙子, 内田英二, 内藤善哉, 日本病理学会会誌, 99, 1, 225,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002203971236389
  • NOGマウスを用いたヒト膵癌培養細胞の転移モデル, 秋山輝, 松田陽子, 藤井雄文, 川原清子, 河本陽子, 手塚潔, 鈴木妙子, 山本哲志, 石渡俊行, 内藤善哉, 日本病理学会会誌, 99, 1, 373,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002206180738002
  • 神経膠芽腫におけるNestinの発現と,その役割の検討, 手塚潔, 石渡俊行, 吉田常恭, 川原清子, 鈴木妙子, 藤井雄文, 河本陽子, 山本哲志, 松田陽子, 内藤善哉, 日本病理学会会誌, 99, 1, 351,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002208825757409
  • 膵癌における各腫瘍血管マーカーと臨床病理学的因子との関連―NestinとCD34陽性血管の比較, 山初和也, 松田陽子, 石渡俊行, 山本哲志, 河本陽子, 手塚潔, 相本隆幸, 中村慶春, 廣井信, 内田英二, 内藤善哉, 日本病理学会会誌, 99, 1, 223,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002224050516545
  • 線維芽細胞増殖因子受容体(FGFR2 IIIc)を標的とした膵癌治療法の研究, 石渡俊行, 松田陽子, 山本哲志, 河本陽子, 川原清子, 恩田宗彦, 内田英二, 内藤善哉, 日本病理学会会誌, 99, 1, 224,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002233361394744
  • 膵臓癌におけるlumicanの発現と増殖への関与, 山本哲志, 石渡俊行, 松田陽子, 川原清子, 恩田宗彦, 内藤善哉, 日本病理学会会誌, 99, 1, 224,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002247315487453
  • ヒト培養癌細胞移植ヌードマウス組織における各種固定法の抗原性保持能の検討, 藤井雄文, 石渡俊行, 松田陽子, 山本哲志, 山初和也, 河本陽子, 手塚潔, 鈴木妙子, 川原清子, 内藤善哉, 日本病理学会会誌, 99, 1, 285,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002263694236522
  • 薄切標本におけるRNA保持に有用な保存法の検討, 河本陽子, 石渡俊行, 川原清子, 鈴木妙子, 松田陽子, 山本哲志, 藤井雄文, 手塚潔, 恩田宗彦, 内藤善哉, 日本病理学会会誌, 99, 1, 275,   2010 03 26 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002280328778926
  • Determination of cadmium by inductively coupled plasma atomic emission spectrometry using a newly designed double chamber electrothermal vaporization system, Akihiro Matsumoto, Shusuke Osaki, Toshitsugu Kobata, Yuji Ohshita, Tetsushi Yamamoto, Bunji Hashimoto, Hiroshi Uchihara, Bunseki Kagaku, 58, 393, 397,   2009 12 22 , 10.2116/bunsekikagaku.58.393, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=72149094047&origin=inward
    Summary:A new improved double-chamber electrothermal vaporization (ETV) system has been designed. An inner chamber made of quartz glass was attached with an inlet port to an inductively coupled plasma (ICP) torch. The inner chamber has an important role to introduce metal vapor in a 1.0 kW argon ICP. Ten microliters sample aliquots were dried at 90°C and subsequently heated at 1300°C on a tungsten boat furnace. The released vapor was swept into the ICP source through PTFE tubing and the inner chamber by 0.6 L min-1 H2 (7%) -Ar carrier gas. The performance parameters of ETV-ICP-AES, such as the temperature program, gas flows and position of the inner chamber, were evaluated by using a cadmium standard solution. Under the optimized stop and flow experimental conditions, the best attainable detection limit at Cd I 214.43 nm line was 0.2 ng mL-1 with linear dynamic ranges of 10 to 10000 ng mL-1 for cadmium without modifiers. The instrumental precision, expressed as the relative standard deviation (RSD) from ten replicate measurements of 10000 ng mL-1 for cadmium by ETV-ICP-AES, was 0.85%. The detection limit and RSD were improved by 3 times over that reported by Okamoto. The present method has been applied to the determination of cadmium in water and plant samples. © 2009 The Japan Society for Analytical Chemistry.
  • 膵臓癌のNestin発現制御による浸潤・転移抑制の研究, 松田陽子, 石渡俊行, 川本聖郎, 山本哲志, 許田典男, 山初和也, 彭為霞, 恩田宗彦, 内田英二, KORC Murray, 内藤善哉, すい臓, 24, 3, 374,   2009 06 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902220186085991
  • 線維芽細胞増殖因子受容体(FGFR2IIIc)発現制御による膵臓癌治療の研究, 石渡俊行, 山本哲志, 松田陽子, 恩田宗彦, 川本聖郎, 内田英二, KORC Murray, 内藤善哉, すい臓, 24, 3, 373,   2009 06 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902224567797179
  • Fibroblast growth factor receptor2 IIIc過剰発現による膵臓癌増殖促進と,その制御による治療応用, 石渡俊行, 松田陽子, 山本哲志, 手塚潔, 藤井雄文, 川原清子, 中澤南堂, 恩田宗彦, 内田英二, 内藤善哉, 日本病理学会会誌, 98, 1, 245,   2009 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902205259491315
  • 大腸癌における線維芽細胞増殖因子受容体Fibroblast growth factor receptor 2IIIc(FGFR2IIIc)の発現と役割, 松田陽子, 恩田宗彦, 川原清子, 河本陽子, 山本哲志, 工藤光洋, 中澤南堂, 瀬谷知子, 大秋美治, 石渡俊行, 内藤善哉, 日本病理学会会誌, 98, 1, 227,   2009 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902216155406970
  • 細胞増殖能へのlumicanの影響とシグナル伝達系の解析, 山本哲志, 石渡俊行, 松田陽子, 川原清子, 河本陽子, 恩田宗彦, 内藤善哉, 日本病理学会会誌, 98, 1, 367,   2009 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902249607095779
  • 膵癌におけるNestinの発現と,その制御による膵癌治療の可能性の検討, 内藤善哉, 松田陽子, 川本聖郎, 山本哲志, 小野ゆり, 恩田宗彦, 内田英二, 石渡俊行, 日本病理学会会誌, 98, 1, 245,   2009 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902280426400663
  • 安定過剰発現株を用いたlumicanの機能解析, 山本哲志, 川原清子, 河本陽子, 藤井雄文, 小野ゆり, 松田陽子, 恩田宗彦, 石渡俊行, 内藤善哉, 日本薬学会年会要旨集, 129th, 3, 80,   2009 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902273033451758
  • 膵臓癌細胞の遊走、浸潤におけるネスチンの役割の検討(Expression of nestin correlates with invasion and migration of pancreatic cancer), 内藤 善哉, 川本 聖郎, 張 一光, 山本 哲志, 松田 陽子, 小野 ゆり, 内田 英二, 田尻 孝, 石渡 俊行, 日本癌学会総会記事, 67回, 100, 100,   2008 09
  • 膵臓癌増殖における線維芽細胞増殖因子受容体2III cアイソフォームの役割(Role of fibroblast growth factor receptor 2 IIIc isoforms in pancreatic cancer cell growth), 石渡 俊行, 川本 聖郎, 山本 哲志, 張 一光, 松田 陽子, 小野 ゆり, 川原 清子, 河本 陽子, 中澤 南堂, 内田 英二, 田尻 孝, 内藤 善哉, 日本癌学会総会記事, 67回, 194, 194,   2008 09
  • 膵臓癌の後腹膜浸潤と予後へのlumicanの関与(Expression of lumican correlates with retroperitoneal invasion and prognosis in pancreatic cancer), 山本 哲志, 石渡 俊行, 川本 聖郎, 小野 ゆり, 松田 陽子, 恩田 宗彦, 内田 英二, 内藤 善哉, 日本癌学会総会記事, 67回, 213, 213,   2008 09
  • 肺癌と癌間質におけるルミカンとその臨床病理学的意義(Lumican in lung cancer cells and stromal tissues: its clinicopathological significance), 松田 陽子, 山本 哲志, 工藤 光洋, 川原 清子, 河本 陽子, 川本 雅司, 中島 ゆき, 小泉 潔, 中澤 南堂, 石渡 俊行, 内藤 善哉, 日本癌学会総会記事, 67回, 274, 275,   2008 09
  • 膵臓癌におけるFibroblast growth factor receptor(FGFR)‐2の発現と役割の検討, 石渡俊行, 張一光, 川本聖郎, 山本哲志, 川原清子, 手塚潔, 松田陽子, 中澤南堂, 内田英二, 田尻孝, 内藤善哉, すい臓, 23, 3, 373,   2008 06 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902201450061333
  • 膵癌におけるNestinの発現と浸潤との関連性, 川本聖郎, 石渡俊行, 内田英二, 相本隆幸, 中村慶春, 廣井信, 張一光, 山初和也, 恩田宗彦, 小野ゆり, 山本哲志, 松田陽子, 内藤善哉, 田尻孝, すい臓, 23, 3, 343,   2008 06 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902206678759391
  • Lumican安定過剰発現株を用いたlumicanの役割の検討, 川原清子, 石渡俊行, 藤井雄文, 手塚潔, 鈴木妙子, 河本陽子, 松田陽子, 山本哲志, 中澤南堂, 内藤善哉, 日本病理学会会誌, 97, 1, 312,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902229897662255
  • 小型ロイシンリッチプロテオグリカンlumicanによる膵臓癌の増殖・浸潤への影響, 山本哲志, 石渡俊行, 川原清子, 藤井雄文, 小野ゆり, 松田陽子, 恩田宗彦, 内藤善哉, 日本病理学会会誌, 97, 1, 274,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902274370887270
  • 膵癌におけるNestinの発現と浸潤能との関連, 川本聖郎, 石渡俊行, 内田英二, 相本隆幸, 中村慶春, 張一光, 恩田宗彦, 工藤光洋, 小野ゆり, 松田陽子, 山本哲志, 川原清子, 藤井雄文, 手塚潔, 河本陽子, 鈴木妙子, 田尻孝, 内藤善哉, 日本病理学会会誌, 97, 1, 252,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902277517012789
  • ケラチノサイト成長因子のトランスフェクションにより刺激された大腸癌細胞の細胞外マトリックスへの接着(Keratinocyte Growth Factor- fransfection-Sfimulated Adhesion of Colorectal Cancer Cells to Extracellular Matrices), 工藤 光洋, 石渡 俊行, 石川 温子, 山本 哲志, 松田 陽子, 小野 ゆり, 恩田 宗彦, 内藤 善哉, 日本癌学会総会記事, 66回, 135, 135,   2007 08
  • 膵癌の静脈侵襲におけるKGF、KGF receptor(KGFR)とMMP-9の関与(Keratinocyte growth factor and its receptor correlates with venous invasion via MMP-9 in pancreatic cancer), 張 一光, 石渡 俊行, 中澤 南堂, 工藤 光洋, 内田 英二, 相本 隆幸, 中村 慶春, 松下 晃, 勝野 暁, 川本 聖郎, 山本 哲志, 内藤 善哉, 田尻 孝, 日本癌学会総会記事, 66回, 562, 562,   2007 08
  • 膵癌におけるnestinの発現とその意義, 川本聖郎, 石渡俊行, 内田英二, 相本隆幸, 中村慶春, 松下晃, 勝野暁, 張一光, 山本哲志, 藤井雄文, 手塚潔, 河本陽子, 内藤善哉, 田尻孝, すい臓, 22, 3, 412,   2007 05 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902248942753164
  • 医薬品卸におけるDI業務と薬剤師の役割~お得意さまのニーズにお応えする為に~, 高橋綾子, 青井依子, 加藤有美子, 菅谷利江, 野々垣幸保, 丸山歸一, 宮崎恵子, 村社昌美, 山本哲志, 日本薬剤師会学術大会講演要旨集, 38th, 218,   2005 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902241609145659
  • ラット肝細胞内リトコール酸修飾蛋白質の捕捉と構造解析, 山本哲志, 伊藤裕美, 石渡俊二, 前田昌子, 後藤順一, 池川繁男, 日本薬学会年会要旨集, 124th, 3, 32,   2004 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902235698699956
  • Rejection of stimulus-related artifacts from electro-gustatory magnetoencephalographic signals using independent component analysis, S. Iwaki, C. Yamamoto, S. Takehara, M. Tonoike, T. Yamamoto, Digests of the Intermag Conference,   2003 10 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=18344417200&origin=inward
    Summary:A study was done on the rejection of stimulus-related artifacts from electro-gustatory magnetoencephalographic signals using independent component analysis (ICA). This approach minimized the mutual information among the output independent components by maximizing the joint entropy of the output of simple neural network. It was found that unlike principal component analysis (PCA) that required the spatial orthogonality of each component, ICA did not impose any condition on the spatial filters.
  • 肝疾患の病態プロテオミクスに用いる分子標的機能性素子の創製, 池川繁男, 山本哲志, 伊藤裕美, 石渡俊二, 真野成康, 後藤順一, 前田昌子, バイオメディカル分析科学シンポジウム講演要旨集, 16th, 86, 87,   2003 08 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902278563717939
  • リトコール酸‐蛋白付加体の検出に用いるモノクローナル抗体の調製, 伊藤裕美, 大西恵, 山本哲志, 石渡俊二, 前田昌子, 真野成康, 後藤順一, 池川繁男, 日本薬学会年会要旨集, 123rd, 3, 55,   2003 03 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902260685364526
  • Detoxification of Chlorinated Aromatics Adsorbed on Fly Ash under Microwave Irradiation, Takamasa Kishima, Tsuyoshi Koizumi, Yoshio Iio, Sumio Tujii, Yuji Wada, Tetsushi Yamamoto, Hengbo Yin, Takayuki Kitamura, Shozo Yanagida, IEICE Transactions on Electronics, E86-C, 2474, 2478,   2003 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0842332052&origin=inward
    Summary:We succeeded in detoxification of hexachlorobenzene adsorbed on artificially produced fly ash in air by irradiating microwave (2.45 GHz) in the presence of activated carbon powder. Hexachlorobenzene was decomposed by 50-90% at 200-300°C by MW irradiation of 1-1.5 min when the ash contained activated carbon by 12 wt% and water by 10 wt%. Chlorinated benzene derivatives are dechlorinated through substitution of chloride anion with hydroxylation produced by basic CaO in the co-presence of activated carbon effectively heated by MW. This method using microwave irradiation enables us to treat the contaminated fly ash in a shorter time and decompose hexachlorobenzene more efficiently than the conventional heating.
  • リトコール酸‐蛋白付加体の捕捉と構造解析を目的とするエピトープ特異抗体の調製, 山本哲志, 伊藤裕美, 池川繁男, 前田昌子, 真野成康, 後藤順一, 日本分析化学会年会講演要旨集, 51st, 265,   2002 09 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902155290447920
  • Role of heme oxygenase-1 and kupffer cells in the production of bilirubin in the rat liver, K. I. Hirano, T. Kobayashi, T. Watanabe, T. Yamamoto, G. Hasegawa, K. Hatakeyama, M. Suematsu, M. Naito, Archives of Histology and Cytology, 64, 169, 178,   2001 01 01 , 10.1679/aohc.64.169, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034979644&origin=inward
    Summary:Heme oxygenase (HO)-1, the heme-degrading enzyme in macrophages, plays a key role in bilirubin metabolism. HO-1 is expressed in various tissue macrophages, especially Kupffer cells. This study aimed to examine the roles of macrophages and HO-1 in the modulation of heme catabolism in rat livers. Rats treated with or without liposome-encapsulated dichloromethylene diphosphonate, a macrophage-depleting reagent, were administered with heat-denatured red blood cells (h-RBC), and the time course of the biliary output of bilirubin and the expressions of HO-1 mRNA and protein were monitored. Immunohistochemistry in the control rat liver revealed that Kupffer cells constitute a major cellular component expressing HO-1, while hepatocytes exhibited little expression. The levels of HO-1 expression in Kupffer cells were elevated immediately after injection of h-RBC. In Kupffer cell-depleted livers, however, HO-l-expressing cells were not detected even after h-RBC administration. HO-1 mRNA levels were elevated at 2 h after administration of h-RBC in control rat livers, while they were very low in Kupffer cell-depleted rat livers. The control and Kupffer cell-depleted groups exhibited distinct time courses of biliary bilirubin excretion. In the untreated control rats, total bilirubin excretion increased about two-fold at 5 h after h-RBC administration. In contrast, the Kupffer cell-depleting treatment decreased the level of bilirubin production; administration of h-RBC to Kupffer cell-depleted rats did not accelerate the generation of bilirubin. These results suggest that Kupffer cells serve both as a sensor for scenesent RBC clearance and an effector that upregulates heme-degrading capacity and bilirubin production.
  • Quantum size effect of CuCI microcrystals-doped SiO2 glass thin films, Hiroyuki Nasu, Tetsushi Yamamoto, Takafumi Iwano, Tadanori Hashimoto, Kanichi Kamiya, Journal of the Ceramic Society of Japan, 106, 1037, 1039,   1998 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0032179774&origin=inward
    Summary:CuCl microcrystals-doped SiO2 glass thin films were successfully prepared by rf (radio frequency)-sputtering method. The formation of CuCl microcrystals in the glass thin films has been explored by X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy. The quantum size effect in CuCl was found from the blue shift of exciton absortion peaks. Although some influence of the matrix on the temperature dependent linewidth of the exciton peaks was observed, the relationship between the amount of blue shift of the absorption edge and the diameter of the CuCl microcrystals satisfied the theory for the quantum-size confinement of the exciton.
  • Geminal delocalization of sigma-electrons and inverted tetrahedral configurations of the bridgehead carbons in bicyclo[1.1.0]butane frameworks, S Inagaki, T Kakefu, T Yamamoto, H Wasada, JOURNAL OF PHYSICAL CHEMISTRY, 100, 23, 9615, 9618,   1996 06 , 10.1021/jp9529432, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0030572325&origin=inward
    Summary:The origin of the inverted tetrahedral configurations of the bridgehead carbons in bicyclo[1.1.0]butane has been investigated in a theoretical manner. The delocalization of sigma-electrons between the central C-1-C-3 bond and the side C-1-C-2 bonds was found to be bonding to relax the angle strains, while the delocalization between the geminal C-C bonds is antibonding in propane and cyclopropane, etc. The bonding property of the geminal delocalization was shown to be related to the inversion of the configurations. Inverted and normal tetrahedral bridgehead configurations in the bicyclo[1.1.0]butane frameworks were designed by controlling the geminal delocalization and confirmed by ab initio molecular orbital calculations.
  • Home terminal care for terminal gastric cancer patients--case of epidural morphine injection, S. Shiratsuchi, H. Suga, M. Uchiyama, T. Yamamoto, M. Fujii, M. Kudo, T. Makihara, Gan to kagaku ryoho. Cancer & chemotherapy, 21 Suppl 4, 497, 500,   1994 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028711819&origin=inward
    Summary:Pain management at home for a terminal gastric cancer patient unable to take medications orally was made possible by a combination of serial morphine drip infusion and epidural anesthesia with morphine on a continuing basis using a disposal syringe. Before the patient was discharged, a conference was held to prepare for home care. Besides the patient and family, it was attended by the primary care doctor, ward nurse, home nurse, pharmacy staff, pain clinic doctor and hospital office personnel. The patient was duly informed of the disease by name and, thanks to fine cooperation from the medical staff, had achieved a good understanding, and spent his remaining time meaningfully. Morphine dosages upon discharge were 80 mg/day by drip infusion and 90 mg/day epidurally. For times of increased pain, the patient was instructed in how to self-administer 0.5% Mepivacaine (5 ml/time) with a syringe using an epidural catheter equipped with a 3-way stopcock. When this proved insufficient to control the pain, the patient was instructed to come for outpatient treatment on an emergency basis. With one visit per week to the hospital and home visits by a nurse once or twice a week, the patient managed at home for 82 days before increased pain resulted in rehospitalization. After the pain was brought under control and the patient was discharged, he was again hospitalized 5 days later. The pain control up until the time of death was by drip infusion of up to 1,200 mg/day morphine.
  • Expression of c-erbB-2 gene product in urinary bladder cancer, M. Moriyama, T. Akiyama, T. Yamamoto, T. Kawamoto, T. Kato, K. Sato, T. Watanuki, T. Hikage, N. Katsuta, S. Mori, Journal of Urology, 145, 423, 427,   1991 01 01 , 10.1016/S0022-5347(17)38356-8, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025978159&origin=inward
    Summary:Expression of the c-erbB-2 gene product and the epidermal growth factor receptor (EGF-R) was investigated in 54 cases of human bladder cancer immunohistologically and by Western blot analysis. For detection of the c-erbB-2 product, two specific antibodies, a rabbit polyclonal antibody directed to the intracellular domain and a murine monoclonal antibody recognizing an epitope in the extracellular domain, were used. Seventeen cases of bladder cancer were stained by the anti-c-erbB-2 polyclonal antibody, while 20 cases were stained by the monoclonal antibody, with good correlation on both stainings (p < 0.01). There were four c-erbB-2 positive cases in 26 G1 tumors, four in 15 G2 tumors, and nine in 13 G3 tumors. There were also eight erbB-2 positive cases in nine muscle-invasive tumors, nine of 45 superficial tumors, four of five with lymph node metastasis, and seven of 14 without metastasis, as revealed by staining with the polyclonal antibody. Thus, the c-erbB-2 gene product was more frequently expressed in high grade tumors (p < 0.01), in high stage tumors (p < 0.01), and nodal metastatic tumors (N.S. by Chi-square test). Twenty-two of the 54 tumors were stained by an anti-EGF-R monoclonal antibody, 528 IgG. The expression of EGF-R was independent of histological grading, tumor stage, and nodal status, and no correlation was observed between expression of the c-erbB-2 product and EGF-R. The c-erbB-2 product may be applicable as a tumor marker for evaluation of malignant potential, invasiveness, and probably metastatic potential of human bladder cancer.
  • Constructive picture description with Euclidean geometry, Tsukasa Noma, Tosivasu L. Kunii, Nami Kin, Hirohisa Enomoto, Emako Aso, Tetsushi Yamamoto, The Visual Computer, 5, 40, 52,   1989 01 01 , 10.1007/BF01901480, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249967720&origin=inward
    Summary:This paper proposes a novel approach to picture description called constructive picture description. In this approach, the points which specify pictures are defined through repetitive geometrical constructions, and the final image is drawn by referring to those points. This approach fulfills the requirements for picture description: easiness, intuitiveness, uniqueness, and less computation. In addition to that, to clarify the feature of our constructive picture description, we discuss the relationship between our proposal and formal elementary plane geometry. We show the usefulness of constructive picture description through three examples: a generalpurpose preprocessor, apparel patternmaking, and Tibetan mandala image generation. © 1989 Springer-Verlag.
  • Epidermoid cyst of the testis: a report of 2 cases, S. Satoh, T. Yamamoto, H. Aoki, I. Fuzizuka, T. Kubo, T. Ohhori, R. Satodate, Hinyokika kiyo. Acta urologica Japonica, 34, 1265, 1268,   1988 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0024043841&origin=inward
    Summary:We report two cases of intratesticular epidermoid cyst, one of which had ossification in the cyst. Two cases were treated by orchiectomy with high ligation of the cord under the diagnosis of testicular malignant tumor. Ultrasonographic examination revealed a well-defined solid mass with echogenic rim. The internal echo of the cysts were relatively homogeneous and almost similar to the surrounding normal testicular parenchyma in the echo level.
  • Epidermoid cyst of the testis: Report of two cases, T. Yamamoto, M. Nakayama, M. Hayakawa, Acta Urologica Japonica, 31, 161, 163,   1985 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0021928402&origin=inward
  • Etiology of the mucocutaneous lymph node syndrome, and the Streptococcus hypothesis, T. Yamamoto, Acta Paediatrica Japonica (Overseas Edition), 25, 118, 126,   1983 01 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0021017416&origin=inward
  • The effect of maple syrup extract on cell proliferation of malignant melanoma cells, Chiaki Kubota, Tetsushi Yamamoto, Atsushi Taga, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 40, S55, S55,   2017
  • The effect of cyclophilin A on cell invasion of colorectal cancer cells, Tetsushi Yamamoto, Kuniko Mitamura, Atsushi Taga, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 40, S52, S52,   2017 , Refereed
  • The Role of Cyclophilin a As a Novel Therapeutic Target of Colorectal Cancer Based on Proteome Analysis Using Formalin-Fixed and Paraffin Embedded Colorectal Cancer Tissue, Hideki Takakura, Tetsushi Yamamoto, Kuniko Mitamura, Mitsuhiro Kudo, Zenya Naito, Atsushi Taga, GASTROENTEROLOGY, 150, 4, S620, S620,   2016 04 , Refereed, 10.1016/S0016-5085(16)32129-1
  • Protein Component in Maple Syrup Has a Potential to Develop Novel Anti-Cancer Drugs for Colorectal Cancer, Tetsushi Yamamoto, Ryota Shiburo, Kuniko Mitamura, Atsushi Taga, GASTROENTEROLOGY, 150, 4, S625, S625,   2016 04 , Refereed, 10.1016/S0016-5085(16)32147-3
  • Proteome analysis of formalin-fixed paraffin-embedded epithelial ovarian cancer tissues, Akane Takaya, Peng Wei-xia, Mitsuhiro Kudo, Tetsushi Yamamoto, Kousuke Ishino, Takenori Fujii, Kiyoshi Tezuka, Ryuichi Wada, Toshiyuki Takeshita, Zenya Naito, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 34, S98, S98,   2014
  • Proteome analysis using formalin-fixed paraffin-embedded keratoacanthoma tissue, Akiko Kanzaki, Mitsuhiro Kudo, Shinnichi Ansai, Tetsushi Yamamoto, Takenori Fujii, Kiyoshi Tezuka, Peng Wie-Xia, Ryuichi Wada, Hidehisa Saeki, Zenya Naito, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 34, S105, S105,   2014
  • Comparison of Fixation Methods for Preservation of Morphology, RNAs, and Proteins From Paraffin-Embedded Human Cancer Cell-Implanted Mouse Models, Yoko Matsuda, Takenori Fujii, Taeko Suzuki, Kazuya Yamahatsu, Kiyoko Kawahara, Kiyoshi Teduka, Yoko Kawamoto, Tetsushi Yamamoto, Toshiyuki Ishiwata, Zenya Naito, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 59, 1, 68, 75,   2011 01 , 10.1369/jhc.2010.957217, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78650819803&origin=inward
    Summary:Xenograft transplantation of human tumor cells into immunodeficient mice is an important method to clarify the roles of specific molecules or chemicals in vivo. Recently, this method has been reported as a definitive examination to identify tumor stem cells. In this study, the authors compared the morphology and the quality and quantity of ribonucleic acid (RNA) and protein in paraffin-embedded tissues of nude mice implanted with human uterine cervical cancer cells, followed by fixation with commonly used fixatives, including 4% paraformaldehyde (PFA), 10% neutral buffered formalin (NBF), 20% NBF, and 99% ethanol (EtOH). The quality of the isolated RNA from PFA- and NBF-fixed paraffin-embedded tissues was high, while EtOH-fixed tissues showed degradation of RNA. NBF-fixed tissues showed excellent quality of morphology, but EtOH-fixed tissues showed contraction of cells. Immunohistochemical results showed differences depending on fixations. The 99% EtOH-fixed samples showed decreases of Ki-67 and VEGF-A immunoreactivities, but improved cytokeratin immunoreactivity. This study indicated that formalin fixation is better than alcohol fixation for RNA preservation in paraffin-embedded cancer cell implantation models. Immunohistochemical results differed markedly depending on fixation materials and antibodies; therefore, suitable fixations are needed to quantify and compare the results of immunohistochemical staining on cancer cell implanted nude mice tissues. (J Histochem Cytochem 59:68-75, 2011)
  • Intralymphatic histiocytosis associated with rheumatoid arthritis, N. Okamoto, M. Tanioka, T. Yamamoto, T. Shiomi, Y. Miyachi, A. Utani, CLINICAL AND EXPERIMENTAL DERMATOLOGY, 33, 4, 516, 518,   2008 07 , 10.1111/j.1365-2230.2008.02735.x, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=45149123939&origin=inward
  • Preparation of luminescent nanosized NaEu(MoO4)(2) incorporated in amorphous matrix originated from zeolite, Shigeru Suzuki, Munenori Ryo, Tetsushi Yamamoto, Takao Sakata, Shozo Yanagida, Yuji Wada, JOURNAL OF MATERIALS SCIENCE, 42, 15, 5991, 5998,   2007 08 , 10.1007/s10853-006-1127-z, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547364629&origin=inward
    Summary:Novel luminescent material has been prepared by the reaction of Eu3+ and molybdate species in the matrix of faujasite (FAU) type zeolite X and successive calcination. Eu3+ exchanged FAU was reacted with MoO3 in the solid-state at 723 K, giving a precursor. By calcining it at 1073 K, different crystalline phases were derived depending on MoO3-loading levels. Scheelite type crystal of NaEu(MoO4)(2) was formed at high MoO3-loading levels, whereas europium sodalite was formed at low loading levels. For the former sample, X-ray diffraction analysis and transmission electron microscopy revealed that the nanosized NaEu(MoO4)(2) was dispersed homogeneously within amorphous aluminosilicate matrix originated from FAU. The amorphous particles containing NaEu(MoO4)(2) maintained the original morphology, which the starting FAU particles possessed. The emission intensity of nanosized NaEu(MoO4)(2) in the matrix was one order higher than that of europium sodalite. The emission lifetime of nanosized NaEu(MoO4)(2) (0.39 ms) in the matrix was longer than that of bulk NaEu(MoO4)(2) (0.35 ms) fabricated by conventional solid-state processes.
  • Morphology-control in microwave-assisted synthesis of silver particles in aqueous solutions, T Yamamoto, HB Yin, Y Wada, T Kitamura, T Sakata, H Mori, S Yanagida, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 77, 4, 757, 761,   2004 04 , 10.1246/bcsj.77.757, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=2342455772&origin=inward
    Summary:The morphologies of Ag particles can be controlled between distorted sphere and prism by employing microwave-promoted reductions of highly concentrated 0.1 M silver nitrate in aqueous solutions. We can selectively synthesize either sphere-Ag or prism-Ag particles by changing only an additive, i.e., either trisodium citrate/formaldehyde or poly(N-vinyl-2-pyrrolidone) (PVP). The particle sizes and size distributions of sphere-Ag particles are strongly dependent on the concentration of trisodium citrate.
  • A novel method for the preparation of green-photoluminescent zinc oxide by microwave-assisted carbothermal reduction, T Yamamoto, Y Wada, H Miyamoto, S Yanagida, CHEMISTRY LETTERS, 33, 3, 246, 247,   2004 03 , 10.1246/cl.2004.246, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=2442672693&origin=inward
    Summary:We succeeded in preparing green-photoluminescent zinc oxide (ZnO) by microwave-assisted carbothermal reduction in air. This method enables us to control the maximum green-PL intensity by changing the percentage of carbon reacted under microwave irradiation for only 2 min at 650 W.
  • Microwave-assisted preparation of silver nanoparticles, T Yamamoto, Y Wada, T Sakata, H Mori, M Goto, S Hibino, S Yanagida, CHEMISTRY LETTERS, 33, 2, 158, 159,   2004 02 , 10.1243/cl.2004.158, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1942532038&origin=inward
    Summary:We succeeded in preparing silver (Ag) nanoparticles by alcohol reduction of fatty acid silver salts under microwave irradiation (2.45 GHz) for 1-5 min at 413-430 K. The average sizes of Ag nanoparticles were controlled in the range from 4.9 to 7.4 nm only by changing length of the alkyl chains in the fatty acids.
  • Large-scale and size-controlled synthesis of silver nanoparticles under microwave irradiation, HB Yin, T Yamamoto, Y Wada, S Yanagida, MATERIALS CHEMISTRY AND PHYSICS, 83, 1, 66, 70,   2004 01 , 10.1016/j.matchemphys.2003.09.006, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0345376102&origin=inward
    Summary:Large-scale and size-controlled silver nanoparticles can be rapidly prepared under microwave irradiation from an aqueous solution of silver nitrate and trisodium citrate in the presence of formaldehyde as a reductant. The size and size distribution of the resulting silver nanoparticles prepared under microwave irradiation are strongly dependent on the states of silver cations in the initial reaction solution. The formation of silver citrate in the initial reaction solution allows for yielding silver nanoparticles with a small size. (C) 2003 Elsevier B.V. All rights reserved.
  • Coronary-coronary bypass and pulmonary artery reconstruction related to Wegener granulomatosis in a 22-year-old woman, A Murakami, N Motomura, T Takaoka, J Kobayashi, K Maeda, T Yamamoto, M Chikada, T Ootsuka, Y Kotsuka, S Takamoto, JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 125, 3, 721, 723,   2003 03 , 10.1067/mtc.2003.141, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037352648&origin=inward
  • Microwave-assisted solvent-free instantaneous Claisen rearrangement for synthesis of bis(3-allyl-4-hydroxyphenyl) sulfone, T Yamamoto, Y Wada, H Enokida, M Fujimoto, K Nakamura, S Yanagida, GREEN CHEMISTRY, 5, 6, 690, 692,   2003 , 10.1039/b311895a, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0347720957&origin=inward
    Summary:Solvent- free Claisen rearrangement of bis( 4- allyloxyphenyl) sulfone ( 1) under microwave irradiation for 5 min gave high yields of bis( 3- allyl- 4- hydroxyphenyl) sulfone ( 3), which has been synthesized up to now under conventional heating for 2 - 30 h as a color developer for a heat- or pressure- sensitive recording in industry.
  • Mycotic pseudoaneurysm of the ascending aorta after mediastinitis in an infant, K Miyaji, N Nagata, H Matsui, T Yamamoto, EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, 22, 4, 638, 639,   2002 10 , 10.1016/S1010-7940(02)00410-4, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0036805641&origin=inward
    Summary:Mycotic pseudoaneurysm of the aorta is a rare disease in children. We report our experience with a postoperative mycotic pseudoaneurysm of the ascending aorta secondary to mediastinitis after a modified Fontan procedure. The pseudoaneurysm was successfully repaired using an autologous pericardial patch through a right thoracotomy under total circulatory arrest. During 6 months of postoperative follow-up, there were no recurrences of pseudoaneurysm formation. (C) 2002 Elsevier Science B.V. All rights reserved.
  • Microwave-driven polyol method for preparation of TiO2 nanocrystallites, T Yamamoto, Y Wada, HB Yin, T Sakata, H Mori, S Yanagida, CHEMISTRY LETTERS, 10, 964, 965,   2002 10 , 10.1246/cl.2002.964, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037027265&origin=inward
    Summary:Anatase TiO2 nanocrystallites have been prepared in 1,4-butanediol, 1,5-pentanediol or 1,6-hexanediol under microwave irradiation by the polyol method. It is demonstrated that the crystallite size less than 10 nm can be controlled by changing the quantity of added water.
  • Apoptosis in adenoma and early adenocarcinoma of the colon, T Yamamoto, N Igarashi, Y Kato, M Kobayashi, M Kawakami, HISTOLOGY AND HISTOPATHOLOGY, 13, 3, 743, 749,   1998 07 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031822257&origin=inward
    Summary:Twenty-six specimens of tubular adenoma and 7 specimens of adenocarcinoma in adenoma of the colon were examined to evaluate apoptosis between adenoma and early adenocarcinoma. Cell proliferation and cell death seemed to be balanced in adenoma with mild and moderate atypia, but unbalanced in adenoma with severe atypia and cancer. Apoptosis was considered to be suppressed at cancer in some cases. However, a number of apoptosis increased at cancer in other cases. Necrosis was seen only in cancer areas. The ratio of cells simultaneously stained by anti-Ki-67 antibody (MIB-1) and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labeling (TUNEL) tended to be high from adenoma with moderate atypia to cancer, suggesting the unstableness of DNA. It is possible that cancer cells having highly unstable DNA easily underwent apoptosis as well as necrosis, accidentally. The p53 protein was positive only in cancer areas of three cases. One of these three cases showed decreased apoptosis in a cancer area, but the other two cases showed increased apoptosis. Furthermore, certain numbers of cancer cells were double-stained by p53 immunohistochemistry and TUNEL. These results suggest that the p53 protein may contribute to suppress apoptosis in the last stage of carcinogenesis of the colonic adenocarcinoma, but other factors including extrinsic stimulation may cause apoptosis despite the mutation of p53 protein.
  • Inverted bond and its effect on the strains of [1.1.1]propellane frameworks, S Inagaki, T Yamamoto, S Ohashi, CHEMISTRY LETTERS, 10, 977, 978,   1997 , 10.1246/cl.1997.977, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0001327801&origin=inward
    Summary:The delocalization of a electrons from a normal bond to a geminal ''inverted'' bond was shown to be strongly bonding due to the orbital phase property. The bonding property of the geminal delocalization was predicted to increase with the bond inversion. The central bond between the bridgehead atoms in the [1.1.1]propellane frameworks (1) is inverted to relax the ring strains as the row of the elements is high in the periodical table. The strain decreases in the order of la(M=C) > Ib (M=Si) > Ic (M=Ge) > 1d (M=Sn).
  • ATP-dependent transport of taurocholate in the canalicular membrane of rat hepatocytes requires magnesium, Y Adachi, H Matsushita, M Kitano, Y Okuyama, H Miya, T Yamamoto, MEDICAL SCIENCE RESEARCH, 23, 12, 833, 834,   1995 12 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0029558518&origin=inward
  • INHIBITION OF THE PROLIFERATION OF ANDROGEN-DEPENDENT SC-3 CELLS BY METHYLCOBALAMIN, Y NISHIZAWA, T YAMAMOTO, N TERADA, B SATO, K MATSUMOTO, Y NISHIZAWA, ONCOLOGY REPORTS, 2, 1, 129, 132,   1995 01 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028872613&origin=inward
    Summary:Methylcobalamin decreased the proliferation of androgen-dependent SC-3 cells, derived from androgen-dependent mouse mammary tumor SC115, in a dose dependent manner at high concentrations without remarkable change of the cell viability. The androgen-induced growth factor activity in the conditioned medium of SC-3 cells cultured in the presence of androgen was shown to be decreased by the addition of methylcobalamin. In SC-3 cells incubated with [methyl-H-3]methylcobalamin, [H-3]methyl moiety was incorporated into the DNA fraction. These results indicate an antiproliferative effect of methylcobalamin on SC-3 cells at high concentrations and suggest a possibility that methylcobalamin can act as a donor of methyl moiety into DNA.
  • PROGRESSION OF MOUSE TRANSFORMED LEYDIG-CELLS FROM ESTROGEN-SENSITIVE TO ESTROGEN-INSENSITIVE GROWTH PHENOTYPE CONCOMITANT WITH LOSS OF LEUKOTRIENE D-4 RECEPTOR, Y NISHIZAWA, Y NISHIZAWA, T YAMAMOTO, N TERADA, Y AMAKATA, K MATSUMOTO, B SATO, INTERNATIONAL JOURNAL OF ONCOLOGY, 5, 5, 1077, 1084,   1994 11 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028053793&origin=inward
    Summary:We have previously shown that growth enhancement of murine transformed Leydig cells (B-1F) by estrogen is partly mediated through inhibition of leukotriene formation due to suppression of 5-lipoxygenase activity; leukotrienes, which inhibit the proliferation of B-1F cells, play an important role in an autocrine loop for B-1F cells to proliferate in an estrogen-sensitive manner. An estrogen-insensitive cell line, termed Cl 4(-), was established from B-1F cells and maintained in serum-free culture medium without addition of estrogen. The proliferation of Cl 4(-) cells was not affected by the addition of 10(-11)-10(-6) M 17 beta-estradiol, whereas an estrogen receptor in Cl 4(-) cells appeared to be normal, as judged by its binding to estrogens. 2-(12-hydroxydodeca-5,10-diynyl)-3,5,6-trimethyl-1,4-benzoquinone (AA861), an inhibitor of 5-lipoxygenase, and indomethacin, a cyclooxygenase inhibitor, did not stimulate the proliferation of Cl 4(-) cells, while AA861 markedly stimulated the proliferation of B-1F cells. Analyses of extracts of medium and cells showed that estrogen and AA861 inhibited the production of leukotriene B-4, C-4, D-4 and E(4) in estrogen-insensitive Cl 4(-) cells as well as in estrogen-sensitive B-1F cells. We were able to demonstrate that no inhibition of the proliferation is observed in Cl 4(-) cells by addition of leukotriene D-4, which inhibited the proliferation of B-1F cells, and that the high affinity binding site for leukotriene D-4 is not present in Cl 4(-) whereas it is present in B-1F cells. These results suggest that the insensitivity of Cl 4(-) cell proliferation to estrogen is at least partly due to deficient binding of leukotriene D-4, since, in Cl 4(-) cells, estrogen inhibits the production of leukotriene D-4, which inhibits the proliferation of estrogen-sensitive parental cell line B-1F.
  • EFFECT OF DEXAMETHASONE ON UTERINE CELL-DEATH, N TERADA, R YAMAMOTO, T YAMAMOTO, Y NISHIZAWA, H TANIGUCHI, N TERAKAWA, Y KITAMURA, K MATSUMOTO, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 38, 1, 111, 115,   1991 01 , 10.1016/0960-0760(91)90410-7, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025972482&origin=inward
    Summary:Oestrogen, progesterone and androgen inhibit uterine cell death after the depletion of oestrogen. In the present study, we investigated effects of glucocorticoid on death of mouse uterine cells. Castrated female mice were given a daily injection of 17-beta-oestradiol (0.2-mu-g/mouse/day) for 3 days, and then an injection of 5'-[I-125]iodo-2'-deoxyuridine ([I-125]IdUrd) to label DNAs of uterine cells with I-125. Mice were killed at intervals during subsequent treatments, and the retention of [I-125]IdUrd incorporated into the whole uterus was determined. On subsequent injection of vehicle only, the I-125-radioactivity retained in the whole uterus rapidly decreased. Injections of dexamethasone (50-mu-g/mouse/day) reduced the loss of I-125-radioactivity slightly but significantly. Dexamethasone also showed synergistic effects on the retention of I-125-radioactivity when it was daily injected together with 17-beta-oestradiol, progesterone or 5-alpha-dihydrotestosterone. The present results suggest that glucocorticoid may affect the processes involved in the uterine cell death, in a manner such as inhibiting the uterine cell death or delaying the removal of DNAs of dead cells from the uterus.
  • EXPERIMENTAL CHEMOTHERAPY AND RADIOTHERAPY TO PARA-TESTICULAR RHABDOMYOSARCOMA, T MOTOYAMA, H WATANABE, T WATANABE, T YAMAMOTO, JOURNAL OF UROLOGY, 141, 1, 187, 189,   1989 01 , 10.1016/S0022-5347(17)40638-0, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0024497251&origin=inward
    Summary:Experimental chemotherapy and radiotherapy were tried in transplanted tumors derived from a paratesticular embryonal rhabdomyosarcoma. There was no significant difference on the therapeutic effect between a combination chemotherapy composed of vincristine, actinomycin D and cyclophosphamide, so-called 'VAC' regimen, and a single therapy of radiation. However, morphologic analyses suggest that VAC is effective in embryonal rhabdomyosarcomas in which undifferentiated rhabdomyoblasts predominate, while radiotherapy is preferable for those containing variously differentiated rhabdomyoblasts.
  • INCIDENCE OF FEMALE BREAST-CANCER AMONG ATOMIC-BOMB SURVIVORS, HIROSHIMA AND NAGASAKI, 1950-1980, M TOKUNAGA, CE LAND, T YAMAMOTO, M ASANO, S TOKUOKA, H EZAKI, NISHIMORI, I, RADIATION RESEARCH, 112, 2, 243, 272,   1987 11 , 10.2307/3577254, https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0023583228&origin=inward
    Summary:Ascertainment of breast cancer incidence among the cohort of the RERF Life Span Study extended sample identified 574 breast cancers among 564 cases diagnosed during 1950-1980 of which 412 cancers were reviewed microscopically. There were no dose-dependent differences with respect to diagnostic certainty or histological type. As in previous studies, the dose reponse appeared to be roughly linear and did not differ between the two cities. The most remarkable new finding was the emergence of a radiation-related excess among women under 10 years of age at exposure. The risk of radiogenic breast cancer appears to decrease with increasing age at exposure, whether expressed in relative or absolute terms. These results suggest that exposure of female breast tissue to ionizing radiation at any time during the first four decades of life, even during the premature stage, can cause breast cancer later in life, and that the length of time that tumor promoters such as endogenous hormones operate following exposure has an important influence on the development of radiation-induced breast cancer. An unresolved question is whether breast cancer risk is increased by radiation exposure at ages older than 40.

Awards & Honors

  •   2019 07 , Asia Pacific Society for Biology and Medical Sciences, Research Award

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(若手研究(B)), Application of control method to inhibit pancreatic cancer cell growth and metastasis by the regulation of lumican glycosylation., Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma(PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PDAC cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PDAC cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.
  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(若手研究(B)), Application for pancreatic cancer therapy to develop control method of keratinocyte growth factor signaling pathway by regulation of lumican expression., Keratinocyte growth factor (KGF) and its receptor, KGFR play important roles in cell growth and invasion in pancreatic cancer. Administration of recombinant KGF to pancreatic cancer cell lines induced cell growth by does-dependent manner, and it also activated ERK and p38, a member of MAPK. Lumican belongs to the proteoglycan family. When we regulated lumican expression levels in pancreatic cancer cell line, we found that there is a positive correlation between lumican expression levels and cell growth, ERK activation. Proteoglycan plays an important role in stabilizing the binding of KGF and KGFR. Therefore, lumican may regulate pancreatic cancer cell growth to regulate KGF/KGFR signaling pathway.