 | OTSUKA AiriDepartment of Life Science Lecturer | ||
Last Updated :2024/04/25
Researcher Information
Degree
J-Global ID
Research Interests
- ストレス緩和 社会敗北性ストレスモデル 心理社会的ストレス
Research Areas
Academic & Professional Experience
- 2019/04 - Today Kindai UniversityFaculty of Science and Engineering, Department of Life Science助教
- 2017/04 - 2019/03 日本学術振興会特別研究員（DC2）
Education
- 2015/04 - 2019/03 The University of Tokushima Graduate School of Medical Sciences
- 2013/04 - 2015/03 The University of Tokushima Graduate School of Nutrition and Bioscience
- 2009/04 - 2013/03 The University of Tokushima Faculty of Medicine School of Nutrition
Association Memberships
Published Papers
- Airi Otsuka; Masaki Tamaya; Asuka TodaPhysiology & behavior 254 113913 - 113913 2022/10Exposure to psychosocial stress is a risk factor for human diseases such as depression. Social defeat stress (SDS) is a well-known rodent model of human psychosocial stress, and animals exposed to SDS show social avoidance behavior. Fish oil, which is rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), is expected to decrease the risk of depressive disorders. In this study, we determined whether fish oil affects the social behavior of SDS-exposed mice and measured serotonin levels and expression of genes related to tryptophan (TRP) metabolism in the hippocampus. The experimental animals were fed a diet containing fish oil during SDS exposure. For the fish oil treatment, experimental mice were fed a diet containing fish oil at low (L-FO), middle (M-FO), and high (H-FO) concentrations. The control group was supplemented with an equivalent amount of canola oil (no fish oil: N-FO). After the SDS protocol, we performed a social interaction test and assessed the sociality of experimental mice. In the N-FO group, SDS-exposed mice showed negative social interactions compared with non-stressed mice. The L-FO and H-FO groups showed negative social interactions after SDS exposure; however, the M-FO group did not exhibit negative social behavior. The serotonin levels of SDS-exposed mice were lower than those of non-stressed mice in the N-FO group. In contrast with these results in the N-FO group, there was no difference in serotonin levels between SDS-exposed and non-stressed mice in the FO groups. In addition, the expression of genes related to TRP metabolism in SDS-exposed mice increased in the N-FO group, but not in the FO group. These results suggest that fish oil improves the psychosocial behavioral disorders caused by SDS. This improvement could be explained by the increase in serotonin synthesis in the hippocampus.
- Airi Otsuka; Yuki Shimomura; Honoka Sakikubo; Kensuke Miura; Nao KagawaFisheries Science Springer Science and Business Media LLC 0919-9268 2021/10 [Refereed]
- Tetsuya Shiuchi; Airi Otsuka; Noriyuki Shimizu; Sachiko Chikahisa; Hiroyoshi SéiInternational journal of molecular sciences 22 (19) 2021/10 [Refereed]
Circadian phase shifts in peripheral clocks induced by changes in feeding rhythm often result in insulin resistance. However, whether the hypothalamic control system for energy metabolism is involved in the feeding rhythm-related development of insulin resistance is unknown. Here, we show the physiological significance and mechanism of the involvement of the agouti-related protein (AgRP) in evening feeding-associated alterations in insulin sensitivity. Evening feeding during the active dark period increased hypothalamic AgRP expression and skeletal muscle insulin resistance in mice. Inhibiting AgRP expression by administering an antisense oligo or a glucocorticoid receptor antagonist mitigated these effects. AgRP-producing neuron-specific glucocorticoid receptor-knockout (AgRP-GR-KO) mice had normal skeletal muscle insulin sensitivity even under evening feeding schedules. Hepatic vagotomy enhanced AgRP expression in the hypothalamus even during ad-lib feeding in wild-type mice but not in AgRP-GR-KO mice. The findings of this study indicate that feeding in the late active period may affect hypothalamic AgRP expression via glucocorticoids and induce skeletal muscle insulin resistance. - Airi Otsuka; Chihomi Nomura; Kensuke Miura; Akira Honda; Nao KagawaZoological Science 37 (5) 2020/09 [Refereed]
In this study, we used the immediate early gene, egr-1, as a marker for neural activation and examined whether egr-1 expression is affected in brain regions associated with the social behavioral network (SBN) when social rank is determined and changed in male medaka fish (Oryzias latipes). Based on the behavioral contest protocol used in this study, we obtained four types of males: social ascending, social descending, dominant, and subordinate. In some brain regions associated with the SBN, we detected higher egr-1 expression in ascending and descending males than in dominant and subordinate males. Social-rank stable males (i.e., dominant and subordinate male fish) showed a similar level of egr-1 expression as the control male fish, which were housed without social stimulus of encountering another conspecific. These findings suggested that the transitioning of social rank could enhance neural activity in some brain regions associated with the SBN in male medaka. The use of medaka fish has many advantages in various fields of research such as genetics, developmental biology, environmental biology, and behavioral neurology. The findings of this study would contribute to future research exploring the roles of the SBN regions in regulating physiological and behavioral events associated with social-rank transition. - Airi Otsuka; Moeko Inahata; Yuki Shimomura; Nao KagawaFisheries Science Springer Science and Business Media LLC 0919-9268 2020/07 [Refereed]
- Airi Otsuka; Kenta Shimomura; Haruka Niwa; Nao KagawaJournal of fish biology 96 (4) 1014 - 1023 2020/04 [Refereed]
Boldness and risk-taking behaviours in animals are important traits to obtain advantages such as habitation, food resources, reproductive success and social dominance. Risk-taking behaviour is influenced by physiological and environmental conditions; however, whether individual fish become bolder by the presence of conspecifics remains unknown. In this study, a light-dark preference test was conducted using medaka fish (Oryzias latipes) with or without a neighbouring conspecific. It was found that individual medaka male fish preferred a light environment and avoided a dark environment, whereas the display of a neighbouring conspecific enhanced the time the male spent in the dark environment (i.e., this condition encouraged risk-taking). The blood glucose level increased in fish confined to the dark condition but did not increase in light-preferring fish and risk-taking fish. Large somata expressing tyrosine hydroxylase, which is the rate-limiting enzyme in dopamine synthesis, were detected in the telencephalic and diencephalic brain regions in risk-taking medaka, whereas large somata were detected in the diencephalic region in medaka confined to the dark condition. These findings indicated that medaka is a good fish model to explore the central roles of dopaminergic neurons in the telencephalon and the diencephalon, which regulate risk-taking behaviour. - 慢性的な軽度ストレスによるマウスの攻撃行動の増加(Chronic mild stress increases aggressive behavior in mice)Chikahisa Sachiko; Shiuchi Tetsuya; Tanioka Daisuke; Shimizu Noriyuki; Otsuka Airi; Sei HiroyoshiThe Journal of Physiological Sciences (一社)日本生理学会 69 (Suppl.1) S139 - S139 1880-6546 2019/06
- Tetsuya Shiuchi; Yumiko Miyatake; Airi Otsuka; Sachiko Chikahisa; Hiroshi Sakaue; Hiroyoshi SéiBiochemical and biophysical research communications 514 (1) 166 - 172 2019/06 [Refereed]
Orexin is known as an important neuropeptide in the regulation of energy metabolism. However, the role of orexin in exercise-induced leptin sensitivity in the hypothalamus has been unclear. In this study, we determined the effect of transient treadmill exercise on leptin sensitivity in the mediobasal hypothalamus (MBH) of mice and examined the role of orexin in post-exercise leptin sensitivity. Treadmill running for 45 min increased the orexin neuron activity in mice. Intraperitoneal injection of a submaximal dose of leptin after exercise stimulated the phosphorylation of signal transducer and activator of transcription 3 (STAT3) in MBH of mice post-exercise compared with that in non-exercised mice, although intracerebroventricular (icv) injection of leptin did not enhance STAT3 phosphorylation, even after exercise. Icv injection of an orexin receptor antagonist, SB334867 reduced STAT3 phosphorylation, which was enhanced by icv injection of orexin but not by direct injection of orexin into MBH. Exercise increased the phosphorylation of extracellular signal-regulated kinases (ERKs) in the MBH of mice, while ERK phosphorylation was reduced by SB334867. Leptin injection after exercise increased the leptin level in MBH, whereas icv injection of SB334867 suppressed the increase in the leptin level in MBH of mice. These results indicate that the activation of orexin neurons by exercise may contribute to the enhancement of leptin sensitivity in MBH. This effect may be mediated by increased transportation of circulating leptin into MBH, with the involvement of ERK phosphorylation. - Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Noriyuki Shimizu; Hiroyoshi SéiLife Sciences Elsevier BV 219 219 - 230 0024-3205 2019/02Aims: Psychosocial stress is a form of mental stress associated with human relationships that underlies the pathogenesis of mental disorders such as depression. Previous studies have suggested that intake of energy-dense foods, also known as "palatable foods," can relieve psychosocial stress. However, it remains unclear whether the volume of palatable food affects abnormal behavior induced by psychosocial stress. In the present study, we aimed to determine whether levels of high-fat food intake significantly influence psychosocial stress using the social-defeat stress (SDS) paradigm. Main methods: Mice subjected to SDS ate either a high-fat or normal chow diet for 10 days. Behavioral tests were conducted following the completion of the SDS paradigm. The hypothalamus, liver, and blood were examined post-mortem. Key findings: Mice with sufficient intake of high-fat chow immediately following exposure to SDS did not exhibit social avoidance behavior, suggesting that a high-fat diet may improve social behavior. However, inadequate intake of high-fat food, which did not alter cholesterol metabolism or hypothalamic-pituitary-adrenal axis activity, was not associated with such benefits, instead increased anxiety-like behavior. Significance: The results of the present study demonstrate that eating a high-fat diet may attenuate stress, but that this benefit disappears with insufficient intake of high-fat foods. The benefits of a high-fat diet under SDS may be related to cholesterol metabolism in the liver.
- Chikahisa S; Chida D; Shiuchi T; Harada S; Shimizu N; Otsuka A; Tanioka D; Séi HBehavioural Brain Research 359 664 - 670 2019/02 [Refereed]
Peroxisome proliferator-activated receptor alpha (PPARα) is a member of the nuclear receptor superfamily and regulates fatty acid oxidation. Although PPARα is expressed not only in the peripheral tissues but also in the brain, its role in higher brain function is unclear. In this study, we investigated the role of PPARα in the control of behavior, including memory/learning and mood change, using PPARα knockout (KO) mice. A significant difference between wild-type (WT) and KO mice was seen in the passive avoidance test, demonstrating that KO mice showed enhanced fear leaning. In the amygdala of KO mice, the levels of dopamine and its metabolites were increased, and the mRNA expression of dopamine degrading enzyme was decreased. When dopamine D1 receptor antagonist was administered, the enhanced fear learning observed in KO mice was attenuated. These results suggest that PPARα is involved in the regulation of emotional memory via the dopamine pathway in the amygdala. - 脳肥満細胞が気分および行動に対して果たす役割(The role of brain mast cells in mood and behavior)Tanioka Daisuke; Chikahisa Sachiko; Shimizu Noriyuki; Shiuchi Tetsuya; Otsuka Airi; Sei HiroyoshiThe Journal of Physiological Sciences (一社)日本生理学会 68 (Suppl.1) S104 - S104 1880-6546 2018/03
- Modification of Energy Metabolism and Higher Brain Function by Feeding RhythmOtsuka Airi; Shiuchi TetsuyaYAKUGAKU ZASSHI 138 (10) 1297 - 1304 2018 [Refereed]
- Sachiko Chikahisa; Saki Harada; Noriyuki Shimizu; Tetsuya Shiuchi; Airi Otsuka; Seiji Nishino; Hiroyoshi SeiSCIENTIFIC REPORTS NATURE PUBLISHING GROUP 7 (1) 2045-2322 2017/10 [Refereed]
We have developed a chronic mild stress (MS) mouse model by simply rearing mice on a wire net for 3 weeks and investigated the effects of MS on glucose homeostasis and sleep. MS mice showed impaired glucose tolerance and disturbed sleep. One-week treatment with a histamine H1 receptor antagonist (H1RA) ameliorated the glucose intolerance and improved sleep quality in MS mice. MS mice showed an increased number of mast cells in both adipose tissue and the brain. Inhibition of mast cell function ameliorated the impairment in both glucose tolerance and sleep. Together, these findings indicate that mast cells may represent an important pathophysiological mediator in sleep and energy homeostasis. - Kanna Oura; Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Noriyuki Shimizu; Hiroyoshi SeiLIFE SCIENCES PERGAMON-ELSEVIER SCIENCE LTD 160 18 - 26 0024-3205 2016/09 [Refereed]
Aims: Sleep and feeding behaviors closely interact to maintain energy homeostasis. While it is known that sleep disorders can lead to various metabolic issues such as insulin resistance, the mechanism for this effect is poorly understood. We thus investigated whether different feeding rhythms during the active period affect sleep-wake regulation. Main methods: For 2 weeks, mice were randomly assigned to 1 of 3 feeding schedules as follows: free access to lab chow during the active period (ZT12-24, Ad-lib group), free access to lab chow during the first half of the active period (ZT12-18; Morning group), or free access to lab chow during the second half of the active period (ZT18-24, Evening group). Food intake, body weight, body temperature, locomotor activity, and sleep were evaluated. The hypothalamus and cerebral cortex were examined post-mortem. Key findings: No alterations in food intake or body weight were observed among the 3 groups. The Evening group showed lower slow-wave activity (SWA) than the other 2 groups, in addition to higher expression of orexin mRNA in the hypothalamus and higher concentrations of dopamine and its metabolites in the cerebral cortex. AMPK phosphorylation was increased in the hypothalamus of mice in the Evening group; however, AMPK inhibition had no effect on SWA. Significance: We concluded that late feeding reduces SWA in NREM sleep via a mechanism that involves orexin-mediated arousal in the hypothalamus and elevated monoamines in the cerebral cortex. These data have important implications for the relationship between sleep-wake disturbances and metabolic disorders. (C) 2016 Elsevier Inc. All rights reserved. - Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Noriyuki Shimizu; Hiroyoshi SeiPHYSIOLOGY & BEHAVIOR PERGAMON-ELSEVIER SCIENCE LTD 151 264 - 271 0031-9384 2015/11 [Refereed]
It is well-established that exercise can influence psychological conditions, cognitive function, and energy metabolism in peripheral tissues including the skeletal muscle. However, it is not clear whether exercise can influence social interaction with others and alleviate defeat stress. This study investigated the effect of voluntary wheel running on impaired social interaction induced by chronic social defeat stress (SDS) using the resident intruder social defeat model. Mice were divided into three groups: control, stress alone, and stress + exercise. SOS was performed by exposing C57BL/6 mice to retired ICR mice for 2.5 min. The C57BL/6 mice were continuously defeated by these resident (aggressor) mice and, following 5 days of SDS, experienced 2 days of rest with no SOS. Mice in the stress + exercise group were allowed to voluntarily run on a wheel for 2 h after every SDS exposure. Two weeks later, compared to the control group, the stress group showed a higher ratio of time spent in the corner zone of a social interaction paradigm even though SDS did not elicit depressive- and anxiety-like behaviors. We also observed that voluntary exercise, which did not affect muscle weight and gene expression, decreased social avoidance behavior of stressed mice without clear changes in brain monoamine levels. Interestingly, food intake in the stress + exercise group was the greatest among the three groups. To test the effect of the exercise-induced increase in food intake on social behavior, we set up a pair-fed group where food intake was restricted. We then compared these mice to mice in the stress alone group. We found that the ratio of time spent in the corner zone of the social interaction test was not different between ad libitum- and pair-fed groups, although pair-fed mice spent more time in the corner zone when an aggressor mouse was present than when it was absent. In addition, pair-feeding did not show exercise-induced reductions of adrenal gland weight and enhanced the loss of body fat. Our findings indicate that voluntary exercise reduces social avoidance behavior induced by SDS. Further, we determined that SOS and exercise-induced increases in food intake partially influence energy metabolism and social avoidance behavior. (C) 2015 Elsevier Inc All rights reserved.
Conference Activities & Talks
- Relationship of stress induced hyperthermia without brown adipose tissue and stress like behavior [Not invited]大塚愛理; 日比宏也; 大河歩路; 濵口葵; 太田菫月第46回日本神経科学大会 2023/08
- 社会敗北性ストレス曝露下における⿂油摂取が社会性⾏動に与える影響 [Not invited]大塚 愛理; 中野 真珠生; 玉谷 將貴; 戸田 飛鳥第77回 栄養・食糧学会大会 2023/05
- 社会敗北性ストレス曝露前の魚油摂餌はストレス様行動を予防する大塚愛理; 中野真珠生; 玉谷將貴; 戸田飛鳥第100回 日本生理学会大会 2023/03
- 社会敗北性ストレス (Social-defeat stress: SDS) 曝露における魚油の摂取のタイミングが 社会的行動と脳内に与える影響 [Not invited]大塚愛理第76回 栄養・食糧学会大会 2022/06
- Fish oil intake reduces social avoidance behavior induced by social defeat stress in mice. [Not invited]Airi OtsukaThe 44th Annual Meeting of Japan Neuroscience Society / The 1st CJK International Meeting 2021/07
- 社会敗北性ストレス (Social-defeat stress: SDS)曝露下における魚油の摂取が社会的行動と脳内に与える影響 [Not invited]大塚 愛理第75回 日本栄養・食糧学会大会 2021/07
- An adequate amount of high-fat diet improves stress-induced social avoidance behavior [Not invited]Airi Otsuka; Tetsuya ShiuchiICoFF 2019/ISNFF 2019 2019/12
- 社会敗北性ストレスとFGF21の関連性の検討 [Not invited]大塚愛理; 志内哲也; 勢井宏義第57回 日本栄養・食糧学会近畿支部大会 2018/12
- FGF21 elevation induced by social-defeat stress is regulated by b-adrenergic receptor [Not invited]大塚愛理; 志内哲也; 勢井宏義第41回 日本神経科学大会 2018/06
- 肝臓におけるエネルギー代謝が迷走神経肝臓枝を介してストレス様行動に与える影響 [Not invited]大塚愛理; 志内哲也第72回 日本栄養・食糧学会 2018/05
- マウスにおける社会敗北性ストレスは血中FGF21を上昇させる [Not invited]大塚愛理; 志内哲也; 近久 幸子; 勢井宏義第95回 日本生理学会大会 2018/03
- Timing and amounts of high fat diet intake affect the benefit which improves social avoidance induced by social defeat stress. [Not invited]大塚愛理; 志内哲也; 勢井宏義IBNS 2017 2017/06
- 社会敗北性ストレス(Social-defeat stress: SDS)において高脂肪食の量及び摂餌タイミングの差が社会行動に与える影響 [Not invited]大塚愛理; 志内哲也; 勢井宏義第71回 日本栄養・食糧学会大会 2017/05
- Social-defeat stress誘発性の社会回避行動は、ストレス直後の高脂肪食摂取により軽減される [Not invited]大塚愛理; 志内哲也; 近久 幸子; 勢井宏義第 68回日本生理学会中国・四国地方会 2016/11
- Restricted high fat diet can improve social avoidance induced by social-defeat stress as same as ad libitum high fat feeding [Not invited]Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Hiroyoshi SéiNeuroscience 2016 2016/11
- 制限された高脂肪食摂取においてもSDS 誘因性の社会回避行動は改善される [Not invited]大塚愛理; 志内哲也; 近久 幸子; 勢井宏義第39 回 日本神経科学大会 2016/07
- Relationship between feeding rhythm and sleep pressure [Not invited]大塚愛理; 大浦寛奈; 志内哲也; 近久 幸子; 勢井宏義第93回 日本生理学会大会 2016/03
- 高脂肪食によるSocial-defeat stress 誘発性の社会回避行動回復メカニズムの検討 [Not invited]大塚愛理; 志内哲也; 勢井宏義第7回 脳科学クラスター・ミニリトリート 2016/02
- 高脂肪食のストレス軽減効果は制限された量でも行動改善を示す [Not invited]大塚愛理; 志内哲也第36 回 日本肥満学会 2015/10
- The effect of exercise on the social-defeat stress [Not invited]大塚 愛理2015 Tokushima Bioscience Retrea 2015/09
- Ingestion of restricted comfortable food improves social avoidance induced by social defeat stress [Not invited]大塚愛理; 志内哲也; 近久 幸子; 寺尾純二; 勢井宏義12th Asian Congress of Nutrition 2015/05
- Physical exercise reduces social avoidance induced by defeat stress [Not invited]大塚愛理; 志内哲也; 近久幸子; 寺尾純二; 勢井宏義第 120 回日本解剖学会総会･全国学術集会・第92 回 日本生理学会大会合同大会 2015/03
- Physical Exercise Improve Social Interaction Induced by Social-Defeat Stress [Not invited]Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Junji Terao; Hiroyoshi SéiExperimental Biology 2015/03
- Social Defeat stress による社会回避行動は短時間の高脂肪食摂取により軽減される [Not invited]大塚愛理; 志内哲也; 近久 幸子; 勢井宏義; 寺尾純二第37 回 日本神経科学大会 2014/09
- 社会敗北性ストレスモデルによる回避行動とその緩和機構 [Not invited]大塚 愛理2014 Tokushima Bioscience Retreat 2014/09
- 摂食リズムの変動がもたらす睡眠・覚醒リズムへの影響とその調節因子の検討 [Not invited]大塚愛理; 大浦寛奈; 志内哲也; 勢井宏義日本睡眠学会 第39 回定期学術集会 2014/07
- 摂食リズムの変動による睡眠深度の変化 [Not invited]大塚愛理; 大浦寛奈; 志内哲也; 勢井宏義; 寺尾純二第68 回 日本栄養・ 食糧学会大会 2014/05
- Effect of feeding patern on sleep depth [Not invited]大塚愛理; 志内哲也; 大浦寛奈; 清水紀之; 近久 幸子; 勢井宏義第91 回 日本生理学会大会 2014/03
MISC
- 谷岡 大輔; 近久 幸子; 清水 紀之; 志内 哲也; 大塚 愛理; 勢井 宏義 日本生理学雑誌 81- (2) 40 -40 2019/05
- 持久的運動により視床下部レプチン感受性が増強するメカニズムの一考察志内 哲也; 宮武 由実子; 大塚 愛理; 阪上 浩; 勢井 宏義 肥満研究 24- (Suppl.) 191 -191 2018/09
- 谷岡 大輔; 近久 幸子; 原田 紗希; 清水 紀之; 志内 哲也; 大塚 愛理; 勢井 宏義 日本生理学雑誌 80- (3) 40 -40 2018/08
- 性腺の摘出は睡眠を変化させる谷岡 大輔; 大上 路生; 近久 幸子; 清水 紀之; 志内 哲也; 大塚 愛理; 勢井 宏義 日本睡眠学会定期学術集会プログラム・抄録集 43回- 231 -231 2018/07
- Airi Otsuka; Tetsuya Shiuchi; Sachiko Chikahisa; Junji Terao; Hiroyoshi Sei FASEB JOURNAL 29- 2015/04
- Tetsuya Shiuchi; Airi Otsuka; Sachiko Chikahisa; Noriyuki Shimizu; Hiroyoshi Sei FASEB JOURNAL 29- 2015/04