KINDAI UNIVERSITY


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KATO Hiromi

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FacultyInstitute of Advanced Technology / Graduate School of Biology-Oriented Science and Technology
PositionProfessor
Degree
Commentator Guidehttps://www.kindai.ac.jp/meikan/884-katou-hiromi.html
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Last Updated :2020/11/25

Education and Career

Education

  •  - 1993 , Kyoto University
  •  - 1993 , Kyoto University, Graduate School of Agriculture
  •  - 1987 , Kyoto University, Faculty of Agriculture
  •  - 1987 , Kyoto University, Faculty of Agriculture

Academic & Professional Experience

  •   2013 , Institute of Advanced Technology, Kindai University
  •   1996 ,  - 1997 , Post Doc,
  •   1997 , - Research Fellow of Japan Society for
  •   1993 ,  - 1996 , Postdoctoral Fellow of Japan Society for
  • the Promotion of Science (Kinki University)
  • Laboratory
  • Animal Reproduction and Biotechnology
  • USA, Colorado State University,
  • the Promotion of Science

Research Activities

Research Areas

  • Life sciences, Animal production science
  • Life sciences, Animal production science

Research Interests

  • Developmental Biotechnology

Published Papers

  • Ubiquitin-proteasome system modulates zygotic genome activation in early mouse embryos and influences full-term development, Highchi C, Shimizu N, Shin SW, Morita K, Nagai K, Anzai M, Kato H, Mitani T, Yamagata K, Hosoi Y, Miyamoto K, Matsumoto K, Journal of Reproduction and Development, Journal of Reproduction and Development, 64(1), 65 - 74, Feb. 2018 , Refereed
  • Large-scale proteomic analysis of Japanese Black cattle: Part III. Identification of protein biomarker candidates for assessment of carcass traits and meat quality characteristics, IKEGAMI HARUKA, NAGAI KOHEI, MATSUHASHI TAMAKO, KOBAYASHI NAOHIKO, TAKEMOTO ATSUSHI, YOSHIHIRO TAKUYA, INOUE ETSUKO, HIGUCHI CHIKA, MORITA KOTARO, UCHIBORI SHO, AMANO TOMOKO, TAGUCHI YOSHITOMO, KATO HIROMI, IRITANI AKIRA, MATSUMOTO KAZUYA, 日本畜産学会報, 日本畜産学会報, 86(2), 141 - 152, May 25 2015
  • Establishment of in situ hybridization technique with mouse single ovum that uses QuantiGene ViewRNA, 16, 35 - 42, Mar. 2011
  • Recovery of cell nuclei from 15,000 years old mammoth tissues and its injection into mouse enucleated matured oocytes, Hiromi Kato, Masayuki Anzai, Tasuku Mitani, Masahiro Morita, Yui Nishiyama, Akemi Nakao, Kenji Kondo, Petr A. Lazarev, Tsuyoshi Ohtani, Yasuyuki Shibata, Akira Iritani, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 85(7), 240 - 247, Jul. 2009 , Refereed
    Summary:Here, we report the recovery of cell nuclei front 14,000-15,000 years old mammoth tissues and the injection of those nuclei into mouse enucleated matured oocytes by somatic cell nuclear transfer (SCNT). From both skin and muscle tissues, cell nucleus-like structures were successfully recovered. Those nuclei were then injected into enucleated oocytes and more than half of the oocytes were able to survive. Injected nuclei were not taken apart and remained its nuclear structure. Those oocytes did not show disappearance of nuclear membrane or premature chromosome condensation (PCC) at 1 hour after injection and did not form pronuclear-like structures at 7 hours after injection. As half of the oocytes injected with nuclei derived from frozen-thawed mouse bone marrow cells were able to form pronuclear-like structures, it might be possible to promote the cell cycle of nuclei from ancient animal tissues by suitable pre-treatment in SCNT. This is the first report of SCNT with nuclei derived from mammoth tissues.
  • Abnormal distribution of chromosomes in the first division of nuclear transferred mouse embryos, Miyuri Kawasumi, Masayuki Anzai, Toshiyuki Takehara, Tasuku Mitani, Hiromi Kato, Kazuhiro Saeki, Akira Iritani, Kazuya Matsumoto, Yoshihiko Hosoi, Journal of Reproduction and Development, Journal of Reproduction and Development, 53(3), 615 - 622, Jun. 2007
    Summary:The majority of somatic cell nuclear transferred (SCNT) embryos die before or after implantation. Many studies have focused on morphological remodeling of the donor nucleus and its associated cytoskeletal structures in the early events of nuclear transfer. However, little is known about the 2-cell stage of SCNT embryos after the first division. In this study, we compared the morphological status of chromosomal division during the 1-cell stage to the 2-cell stage in SCNT embryos with that in intracytoplasmic sperm injection (ICSI) embryos. The microtubules and cytoplasmic asters, which are related to chromatin segregation, disappeared at the pronuclear stage, although formation of the first mitotic spindle was normal in both the SCNT and ICSI embryos. However, nuclear fragmentation was observed in 30% of the 2-cell SCNT embryos and 12% of the 2-cell ICSI embryos. Nuclear fragmentation was present in both blastomeres of these embryos. No apoptotic DNA fragmentation was observed in TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) assays for either the SCNT or ICSI embryos. In both the SCNT and ICSI embryos, the distribution of chromosomes in the first mitotic spindle was disturbed during the process of division from the 1-cell stage to the 2-cell stage. These results suggest that loss of SCNT embryos just before or after implantation may be due to an abnormal chromosome distribution at the 2-cell stage.
  • Expression of transcription factor Cdx2 and Oct3/4 in mouse somatic nuclear transfer embryos., Memoirs of Institute of advanced Technology, Kinki University, Memoirs of Institute of advanced Technology, Kinki University, 12, 33 - 42, Mar. 2007
  • Development of an examination method of a methylation state of bull sperm DNA, Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 12, 43 - 50, Mar. 2007
  • In vitro culture of CD9- and α6-integrin-expressingcells enriched by magnetic cell sorting from cryptorchid adult and pup testes in mice., Memoirs of Institute of Advanced technology, Kinki University, Memoirs of Institute of Advanced technology, Kinki University, 11, 23 - 34, Mar. 2006
  • Localization of the autoimmune regulator (aire) protein in the gonads and embryonic stem cells in mice., Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 11, 15 - 21, Mar. 2006
  • Methylation og the 5'-upstream region og the H19 gene in mouse somatic cell, gametes, wild type and androgenetic ES cells., Memoirs of Institute of Advanced Technology, Kinki University, Memoirs of Institute of Advanced Technology, Kinki University, 11, 41 - 49, Mar. 2006
  • Methylation of the 5’-upstream region of the H19 gene in mouse somatic cell, gametes, wild type and androgenetic ES cells, Reproduction, Fertility and Development, Reproduction, Fertility and Development, 17(1,2), 261 - 262, Jan. 2005
  • Optimization of culture medium for bovine embryos recinstructed with bovine fibroblasts, Mem. Inst. Adv. Tech, Kinki Univ., Mem. Inst. Adv. Tech, Kinki Univ., 10, 63 - 68, 2005
  • DNA Sequence Retrieval from Ancient Animal Skin Tissue Excavated from Siberian Permafrost, 55(1), 388, Jan. 2001
  • Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes, Kohtaro Morita, Mikiko Tokoro, Yuki Hatanaka, Chika Higuchi, Haruka Ikegami, Kouhei Nagai, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Yoshitomo Taguchi, Kazuo Yamagata, Yoshihiko Hosoi, Kei Miyamoto, Kazuya Matsumoto, Journal of Reproduction and Development, Journal of Reproduction and Development, 64(2), 161 - 171, 2018 , Refereed
    Summary:Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2O2). We here report the cellular localization of hyperoxidized PRDX and its involvement in the antioxidant mechanisms of freshly fertilized oocytes. Treatment of zygotes at the pronuclear stage with H2O2 enhanced pronuclear localization of hyperoxidized PRDX in zygotes and concurrently impaired the generation of 5-hydroxymethylcytosine (5hmC) on the male genome, which is an epigenetic reprogramming event that occurs at the pronuclear stage. Thus, our results suggest that endogenous PRDX is involved in antioxidant mechanisms and epigenetic reprogramming during MZT.
  • Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms, Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto, Journal of Reproduction and Development, Journal of Reproduction and Development, 62(6), 599 - 606, 2016 , Refereed
    Summary:Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy. The correlation among ultrastructural head abnormality in sperms from the upper layer of swim-up, fertilization in in vitro fertilization, and pregnancy after embryo transfer was also investigated. Furthermore, sperms with DNA fragmentation in the samples processed via a combination of DGC and swim-up was assessed in a sperm chromatin structure assay. Ultrastructural abnormalities in sperm heads and tails in the upper layer after swim-up following DGC was the lowest among the three groups. Sperms with nuclear vacuoles were the most difficult to eliminate using a combination of DGC and swim-up in all types of head abnormalities. A negative correlation was confirmed between the fertilization rates of intracytoplasmic sperm injection and head abnormality of sperms obtained from the upper layer of the swim-up following DGC. Sperms with DNA fragmentation were effectively decreased using the combination of two techniques. In conclusion, the combination of DGC and swim-up effectively decreased the number of sperms with ultrastructural abnormalities both in the head and in the tail. However, sperms with ultrastructural abnormalities that cannot be completely decreased using a combination of DGC and swim-up may impair fertilization in some cases of intracytoplasmic sperm injection.
  • Possible Role of ZPAC, Zygote-specific Proteasome Assembly Chaperone, During Spermatogenesis in the Mouse, Natsumi Shimizu, Kimihiro Ueno, Ena Kurita, Seung-Wook Shin, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Satoshi Kishigami, Hiromi Kato, Tasuku Mitani, Yoshihiko Hosoi, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 60(3), 179 - 186, Jun. 2014 , Refereed
    Summary:In the mammalian testis, the ubiquitin-proteasome system plays important roles in the process that promotes the formation of mature sperm. We recently identified zygote-specific proteasome assembly chaperone (ZPAC), which is specifically expressed in the mouse gonads and zygote. ZPAC mediates a unique proteasome assembly pathway in the zygote, but the expression profile and function of ZPAC in the testis is not fully understood. In this study, we investigated the possible role of ZPAC during mouse spermatogenesis. First, we analyzed the expression of ZPAC and 20S proteasome subunit alpha 4/PSMA7 in the adult mouse testis. ZPAC and alpha 4 were expressed in spermatogonia, spermatocytes, and round spermatids. In elongating spermatids, ZPAC was expressed until step 10, whereas expression of alpha 4 persisted until step 12. We then examined the expression profile of ZPAC and alpha 4 in a mouse model of experimental unilateral cryptorchidism. Consistent with appearance of morphologically impaired germ cells following cryptorchidism, the ZPAC protein level was significantly decreased at 4 days post induction of experimental cryptorchidism (D4) compared with the intact testis, although the amount of alpha 4 protein persisted at least until D10. Moreover, intense ZPAC staining was co-localized with staining of annexin V, an early indicator of apoptosis in mammalian cells, in germ cells of cryptorchid testis, but ZPAC was also expressed in germ cells showing no detectable expression of annexin V. These results suggest that ZPAC plays a role during spermatogenesis and raises the possibility that 20S proteasome mediated by ZPAC may be involved in the regulation of germ cell survival during spermatogenesis.
  • Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei, Takayuki Yamochi, Yuta Kida, Noriyoshi Oh, Sei Ohta, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Satoshi Kishigami, Tasuku Mitani, Kazuya Matsumoto, Kazuhiro Saeki, Makoto Takenoshita, Akira Iritani, Yoshihiko Hosoi, ZYGOTE, ZYGOTE, 21(4), 358 - 366, Nov. 2013 , Refereed
    Summary:Interspecies somatic cell nuclear transfer (ISCNT) has been proposed as a technique to produce cloned offspring of endangered species as well as to investigate nucleus-cytoplasm interactions in mammalian embryo. However, it is still not known which embryo culture medium is optimal for ISCNT embryos for the nuclear donor or the oocyte recipient. We assessed the effects of the culture medium on the developmental competence of the ISCNT embryos by introducing cynomolgus monkey (Macaca fascicularis) fibroblast nuclei into enucleated rabbit (Oryctolagus cuniculus) oocytes (monkey-rabbit embryo). The monkey-rabbit ISCNT embryos that were cultured in mCMRL-1066 developed to the blastocyst stage, although all monkey-rabbit ISCNT embryos cultured in M199 were arrested by the 4-cell stage. When monkey-rabbit ISCNT and rabbit-rabbit somatic cell nuclear transfer (SCNT) embryos were cultured in mCMRL-1066, the blastocyst cell numbers of the monkey-rabbit ISCNT embryos corresponded to the cell numbers of the control rabbit-rabbit SCNT embryos, which were produced from a rabbit fibroblast nucleus and an enucleated rabbit oocyte. In addition, the presence of mitochondria, which were introduced with monkey fibroblasts into rabbit recipient cytoplasm, was confirmed up to the blastocyst stage by polymerase chain reaction (PCR). This study demonstrated that: (1) rabbit oocytes can reprogramme cynomolgus monkey somatic cell nuclei, and support preimplantation development; (2) monkey-rabbit ISCNT embryos developed well in monkey culture medium at early embryonic developmental stages; (3) the cell number of monkey-rabbit ISCNT embryos is similar to that of rabbit-rabbit SCNT embryos; and (4) the mitochondrial fate of monkey-rabbit ISCNT embryos is heteroplasmic from the time just after injection to the blastocyst stage that has roots in both rabbit oocytes and monkey fibroblasts.
  • GSE Is a Maternal Factor Involved in Active DNA Demethylation in Zygotes, Yuki Hatanaka, Natsumi Shimizu, Satoshi Nishikawa, Mikiko Tokoro, Seung-Wook Shin, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Yoshihiko Hosoi, Satoshi Kishigami, Kazuya Matsumoto, PLOS ONE, PLOS ONE, 8(4), e60205, Apr. 2013 , Refereed
    Summary:After fertilization, the sperm and oocyte genomes undergo extensive epigenetic reprogramming to form a totipotent zygote. The dynamic epigenetic changes during early embryo development primarily involve DNA methylation and demethylation. We have previously identified Gse (gonad-specific expression gene) to be expressed specifically in germ cells and early embryos. Its encoded protein GSE is predominantly localized in the nuclei of cells from the zygote to blastocyst stages, suggesting possible roles in the epigenetic changes occurring during early embryo development. Here, we report the involvement of GSE in epigenetic reprogramming of the paternal genome during mouse zygote development. Preferential binding of GSE to the paternal chromatin was observed from pronuclear stage 2 (PN2) onward. A knockdown of GSE by antisense RNA in oocytes produced no apparent effect on the first and second cell cycles in preimplantation embryos, but caused a significant reduction in the loss of 5-methylcytosine (5 mC) and the accumulation of 5-hydroxymethylcytosine (5 hmC) in the paternal pronucleus. Furthermore, DNA methylation levels in CpG sites of LINE1 transposable elements, Lemd1, Nanog and the upstream regulatory region of the Oct4 (also known as Pou5f1) gene were clearly increased in GSE-knockdown zygotes at mid-pronuclear stages (PN3-4), but the imprinted H19-differential methylated region was not affected. Importantly, DNA immunoprecipitation of 5 mC and 5 hmC also indicates that knockdown of GSE in zygotes resulted in a significant reduction of the conversion of 5 mC to 5 hmC on LINE1. Therefore, our results suggest an important role of maternal GSE for mediating active DNA demethylation in the zygote.
  • Deposition of Acetylated Histones by RNAP II Promoter Clearance May Occur at Onset of Zygotic Gene Activation in Preimplantation Mouse Embryos, Mikiko Tokoro, Seung-Wook Shin, Satoshi Nishikawa, Hyang-Heun Lee, Yuki Hatanaka, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Masayuki Anzai, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56(6), 607 - 615, Dec. 2010 , Refereed
    Summary:We investigated the contribution of phosphorylated RNA polymerase II (RNAP II) and dynamic epigenetic changes to the onset of minor zygotic gene activation (ZGA) Using immunofluorescence staining, we observed that the nuclear Localization of RNAP IT was initiated by 6 hours post insemination (hpi), whereas RNAP II phosphorylated at serine residue 5 of the carboxyl-terminal domain (CTD) was localized by 9 hpi, and then RNAP II phosphorylated at serine residue 2 of the CTD was localized in the nucleus of embryos by 12 hpi In a transient gene expression assay using a plasmid reporter gene (p beta-actin/luciferase+/SV40) injected during 6-9 hpi into the male pronucleus, the luciferase+gene was actively transcribed and translated by 13 and 15 hpi, respectively, indicating that a transcriptionally silent state persisted for it least 4 hours after injection We found that the methylation status in the chicken beta-actin promoter region of the plasmid reporter gene may not be associated with the transcriptionally silent state before minor ZGA Exposure to trichostatin A did not induce premature expression of the silent reporter gene injected into 1-cell embryos containing histone deacetylase activity and did not affect the amount of luciferase produced per embryo Acetylated histone H3 lysine 9/14 and acetylated histone H4 lysine 12 and 16 were enriched preferentially in the injected reporter gene at least until 13 hpi, which coincided with the transcriptionally active state Taken together, these results suggest that deposition 01 selectively acetylated histones onto the chromatin of 1-cell embryos functions together with transcriptional elongation by RNAP II and that this sequential chromatin remodeling is involved in the molecular mechanism associated with the onset of minor ZGA in the preimplantation mouse embryo
  • Inhibition of the Ubiquitin-proteasome System Leads to Delay of the Onset of ZGA Gene Expression, Seung Wook Shin, Mikiko Tokoro, Satoshi Nishikawa, Hyang-Heun Lee, Yuki Hatanaka, Takuji Nishihara, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56(6), 655 - 663, Dec. 2010 , Refereed
    Summary:In mammalian oocytes, the ubiquitin-proteasome system (UPS) is suggested to play important roles in oocyte meiosis resumption, spindle assembly, polar body emission and pronuclear formation by regulating cyclin B1 degradation Ho Never, little is known about the direct relationship between zygotic gene activation (ZGA) and degradation of maternal proteins Here, we investigated the role of the UPS in the onset of ZGA in early mouse embryo First, we found degradation of cyclin B1 protein in fertilized oocytes at 1 hpi by western blot analysis and used the se oocytes throughout this study Subsequently, we determined optimal experimental conditions for transient inhibition of proteasomal activity by specific and reversible proteasomal inhibitor MG132 in the Cl phase of the first cell cycle Under the selected optimal conditions, we subjected transient MG132-treated embryos to reverse transcription (RT)-PCR analysis of expression of four ZGA genes, i e, the hsp70 1, MuERV-L, eif-1a and zscan4d genes As a result, we found that onset of expression of the four examined ZGA genes was delayed m both normally developed 2-cell embryos and arrested 1-cell embryos Our results indicate that proteasomal degradation of proteins by the UPS plays a pivotal role in the molecular mechanisms of ZGA in early mouse embryos
  • Expression analysis of circadian genes in oocytes and preimplantation embryos of cattle and rabbits, Tomoko Amano, Kaori Tokunaga, Reiko Kakegawa, Ayaka Yanagisawa, Atsushi Takemoto, Atsuhiro Tatemizo, Tatsuya Watanabe, Yuki Hatanaka, Akinori Matsushita, Masao Kishi, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, ANIMAL REPRODUCTION SCIENCE, ANIMAL REPRODUCTION SCIENCE, 121(3-4), 225 - 235, Sep. 2010 , Refereed
    Summary:We previously showed that circadian genes clock, bmal1, cry1, cry2, per1, and per2 are expressed and function as maternal mRNA regulating events in the oocytes and preimplantation embryos of mice. Recent evidence indicates however that either or both expression profiles of circadian genes in some tissues, and transcript sequences of circadian genes, differ to generate the physiological differences between diurnal and nocturnal species. We therefore investigated the expression profiles of circadian genes in oocytes and preimplantation embryos of species other than mice, namely cattle and rabbits, representing diurnal and nocturnal species, respectively, and determined the protein sequences of circadian genes in these species. Quantitative real-time PCR revealed that all circadian genes considered in this study were present in the oocytes and preimplantation embryos of both species, and the transcript amounts of clock, cry1 and per1 contained in oocytes were significantly higher than in preimplantation embryos of both species. The transcripts of clock, cry1, and per1 of cattle and rabbits were determined by primer walking, and functional domains in the estimated amino acid sequences were compared between cattle and rabbits and with those of humans and mice. The sequences of clock, cry1, and per1 in cattle and rabbits closely resembled those in mice (85-100% homologies), and no difference based on diurnality or nocturnality was observed. These findings suggest that circadian genes in the oocytes and preimplantation embryos of mammals fulfill the same functions across species as maternal mRNA. (C) 2010 Elsevier B.V. All rights reserved.
  • Mouse Androgenetic Embryonic Stem Cells Differentiated to Multiple Cell Lineages in Three Embryonic Germ Layers In Vitro, Takeshi Teramura, Yuta Onodera, Hideki Murakami, Syunsuke Ito, Toshihiro Mihara, Toshiyuki Takehara, Hiromi Kato, Tasuku Mitani, Masayuki Anzai, Kazuya Matsumoto, Kazuhiro Saeki, Kanji Fukuda, Norimasa Sagawa, Yoshihiko Hosoi, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 55(3), 283 - 292, Jun. 2009 , Refereed
    Summary:The embryos of some rodents and primates can precede early development without the process of fertilization; however, they cease to develop after implantation because of restricted expressions of imprinting genes. Asexually developed embryos are classified into parthenote/gynogenote and androgenote by their genomic origins. Embryonic stem cells (ESCs) derived from asexual origins have also been reported. To date, ESCs derived from parthenogenetic embryos (PgESCs) have been established in some species, including humans, and the possibility to be alternative sources for autologous cell transplantation in regenerative medicine has been proposed. However, some developmental characteristics, which might be important for therapeutic applications, such as multiple differentiation capacity and transplantability of the ESCs of androgenetic origin (AgESCs) are uncertain. Here, we induced differentiation of mouse AgESCs and observed derivation of neural cells, cardiomyocytes and hepatocytes in vitro. Following differentiated embryoid body (EB) transplantation in various mouse strains including the strain of origin, we found that the EBs Could engraft in theoretically MHC-matched strains. Our results indicate that AgESCs possess at least two important characteristics, multiple differentiation properties in vitro and transplantability after differentiation, and suggest that they can also serve as a source of histocompatible, tissues for transplantation.
  • Abnormal DNA Methylation of the Oct-4 Enhancer Region in Cloned Mouse Embryos, Miyuri Kawasumi, Yuichi Unno, Toshiki Matsuoka, Megumi Nishiwaki, Masayuki Anzai, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Satoshi Kishigami, Kazuya Matsumoto, MOLECULAR REPRODUCTION AND DEVELOPMENT, MOLECULAR REPRODUCTION AND DEVELOPMENT, 76(4), 342 - 350, Apr. 2009 , Refereed
    Summary:Oct-4 is essential for normal embryonic development, and abnormal Oct-4 expression in cloned embryos contributes to cloning inefficiency. However, the causes of abnormal Oct-4 expression in cloned embryos are not well understood. As DNA methylation in regulatory regions is known to control transcriptional activity, we investigated the methylation status of three transcriptional regulatory regions of the Oct-4 gene in cloned mouse embryos-the distal enhancer (DE), the proximal enhancer (PE), and the promoter regions. We also investigated the level of Oct-4 gene expression in cloned embryos. Immunochemistry revealed that 85% of cloned blastocysts expressed Oct-4 in both trophectoderm and inner cell mass cells. DNA methylation analysis revealed that the PE region methylation was greater in cloned morulae than in normal morulae. However, the same region was less methylated in cloned blastocysts than in normal blastocysts. We found abnormal expression of de novo methyltransferase 3b in cloned blastocysts. These results indicate that cloned embryos have aberrant DNA methylation in the CpG sites of the PE region of Oct-4, and this may contribute directly to abnormal expression of this gene in cloned embryos.
  • Expression and Functional Analyses of Circadian Genes in Mouse Oocytes and Preimplantation Embryos: Cry1 Is Involved in the Meiotic Process Independently of Circadian Clock Regulation, Tomoko Amano, Akinori Matsushita, Yuki Hatanaka, Tatsuya Watanabe, Katsutaka Oishi, Norio Ishida, Masayuki Anzai, Tasuku Mitani, Hiromi Kato, Satoshi Kishigami, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, BIOLOGY OF REPRODUCTION, BIOLOGY OF REPRODUCTION, 80(3), 473 - 483, Mar. 2009 , Refereed
    Summary:In mammals, circadian genes, Clock, Arntl (also known as Bmal1), Cry1, Cry2, Per1, Per2, and Per3, are rhythmically transcribed every 24 h in almost all organs and tissues to tick the circadian clock. However, their expression and function in oocytes and preimplantation embryos have not been investigated. In this study we found that the circadian clock may stop in mouse oocytes and preimplantation embryos. Real-time PCR analysis revealed the presence of transcripts of these genes in both oocytes and preimplantation embryos; however, their amounts did not oscillate every 24 h in one- to four-cell and blastocyst-stage embryos. Moreover, immunofluorescence analyses revealed that CLOCK, ARNTL, and CRY1 were localized similarly in the nuclei of germinal vesicle (GV) oocytes and one-cell- to four-cell-stage embryos. Because CRY1 is known to interact with the CLOCK-ARNTL complex to suppress transcription-promoting activity of the complex for genes such as Wee1, Cry2, Per1, Per2, and Per3 in cells having the ticking circadian clock, we hypothesized that if the circadian clock functions in GV oocytes and one-cell- to four-cell-stage embryos, CLOCK, ARNTL, and CRY1 might suppress the transcription of these genes in GV oocytes and one-cell- to 4-cell-stage embryos as well. As a result, knockdown of CRY1 in GV oocytes by RNA interference did not affect the transcription levels of Wee1, Cry2, Per1, Per2, and Per3, but it reduced maturation ability. Thus, it seems that circadian genes are not involved in circadian clock regulation in mouse oocytes and preimplantation embryos but are involved in physiologies, such as meiosis.
  • Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone H1oo, Nucleoplasmin 2, and Zygote arrest 1) are required for oocyte-specific gene expression in the mouse, Kazunobu Tsunemoto, Masayuki Anzai, Toshiki Matsuoka, Mikiko Tokoro, Seung-Wook Shin, Tomoko Amano, Tasuku Mitani, Hiromi Kato, Yoshihiko Hosoi, Kazuhiro Saeki, Akira Iritani, Kazuya Matsumoto, MOLECULAR REPRODUCTION AND DEVELOPMENT, MOLECULAR REPRODUCTION AND DEVELOPMENT, 75(7), 1104 - 1108, Jul. 2008 , Refereed
    Summary:We examined the promoter activities of three mouse maternal genes (H1oo, Npm2, and Zar1) in oocytes and pre-implantation embryos, and examined the promoters for cis-acting elements of 5'-flanking region to obtain the best promoter for inducing oocyte-specific gene expression. For the assay, we injected firefly luciferase gene constructs under the control of the promoters into the oocytes and embryos. Each promoter region showed transcriptional activity in oocytes, but not in fertilized embryos. Deletion analysis showed that a putative E-box region at position -72 of the H1oo promoter and at the -180 of the Npm2 promoter were required for basal transcriptional activity in oocytes. Moreover, a putative NBE motif (NOBOX DNA binding elements) (-1796) was shown to enhance basal transcriptional activity of the Npm2 promoter. Thus, the E-box and/or NBE may be key regulatory regions for the expression of the examined maternal genes (H1oo and Npm2) in growing mouse oocytes.
  • Identification of ZAG1, a novel protein expressed in mouse prei rn plantation, and its putative roles in zygotic genome activation, Toshiki Matsuoka, Manabu Sato, Mikiko Tokoro, Seung-Wook Shin, Atsuto Uenoyama, Kazunari Ito, Syuji Hitomi, Tomoko Amano, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Kazuya Matsumoto, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 54(3), 192 - 197, Jun. 2008 , Refereed
    Summary:We isolated a mouse cDNA, zag1 (zygotic gene activation-associated gene 1), that has an open reading frame of 1,728-bp encoding a protein of 66.2 kDa including both a bipartite nuclear targeting sequence and a P-loop motif containing nucleoside triphosphate hydrolase motifs. Northern blot analysis of mouse tissues showed that zag1 was widely expressed but was especially prominent in the ovary and testis. RT-PCR analysis of in vitro fertilized embryos showed that the abundance of zag1 transcripts in oocytes decreased after fertilization, and zag1 mRNA was detected at 15 h post insemination (hpi) in fertilized embryos indicating that the gene was expressed at the start of zygotic gene activation at the mouse 1-cell stage. The nuclear-localization of ZAG1 protein in mouse preimplantation embryos at 15 hpi was confirmed by both subcellular analysis of enhanced green fluorescent protein (EGFP)-tagged ZAG1 and immunocytochemical analysis with anti-ZAG1 antibody. Subsequently, using yeast two-hybrid screening, we identified U2 small nuclear ribonucleoprotein B (U2B"), which is associated with pre-mRNA splicing, as a putative interacting partner of ZAG1 protein. Furthermore, knockdown of zag1 expression by an antisense DNA plasmid induced arrest and/or delay of embryonic development in injected 1-cell embryos. These results suggest that ZAG1 may be closely associated with zygotic gene expression in mouse preimplantation embryos.
  • Abnormal distribution of chromosomes in the first division of nuclear transferred mouse embryos, Miyuri Kawasumi, Masayuki Anzai, Toshiyuki Takehara, Tasuku Mitani, Hiromi Kato, Kazuhiro Saeki, Akira Iritani, Kazuya Matsumoto, Yoshihiko Hosoi, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(3), 615 - 622, Jun. 2007 , Refereed
    Summary:The majority of somatic cell nuclear transferred (SCNT) embryos die before or after implantation. Many studies have focused on morphological remodeling of the donor nucleus and its associated cytoskeletal structures in the early events of nuclear transfer. However, little is known about the 2-cell stage of SCNT embryos after the first division. In this study, we compared the morphological status of chromosomal division during the 1-cell stage to the 2-cell stage in SCNT embryos with that in intracytoplasmic sperm injection (ICSI) embryos. The microtubules and cytoplasmic asters, which are related to chromatin segregation, disappeared at the pronuclear stage, although formation of the first mitotic spindle was normal in both the SCNT and ICSI embryos. However, nuclear fragmentation was observed in 30% of the 2-cell SCNT embryos and 12% of the 2-cell ICSI embryos. Nuclear fragmentation was present in both blastomeres of these embryos. No apoptotic DNA fragmentation was observed in TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) assays for either the SCNT or ICSI embryos. In both the SCNT and ICSI embryos, the distribution of chromosomes in the first mitotic spindle was disturbed during the process of division from the 1-cell stage to the 2-cell stage. These results suggest that loss of SCNT embryos just before or after implantation may be due to an abnormal chromosome distribution at the 2-cell stage.
  • Application of laser-assisted zona drilling to in vitro fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse, Masayuki Anzai, Megumi Nishiwak, Miho Yanagi, Tatsuyuki Nakashima, Takehito Kaneko, Yoshitomo Taguchi, Mikiko Tokoro, Seung-Wook Shin, Tasuku Mitani, Hiromi Kato, Kazuya Matsumoto, Naomi Nakagata, Akira Iritani, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(5), 601 - 606, Oct. 2006 , Refereed
    Summary:Development of assisted reproductive technologies is necessary to obtain fertilized oocytes in a subfertile transgenic mouse strain. Here, we showed the application of laser-assisted drilling of the zona pellucida to in vitro fertilization of cryopreserved mouse oocytes with sperm from subfertile transgenic mice (C57BL/6N-Tg(UCP/FAD2)U8 strain). After cryopreservation by vitrification, the recovery and survival rates of the zona-drilled mouse oocytes were 97% (97/100) and 94% (91/97), respectively. In vitro fertilization of the cryopreserved zona-drilled mouse oocytes with sperm from the subfertile transgenic mice was greatly facilitated (60%, 55/91) compared to that of the cryopreserved zona-intact mouse oocytes (11%, 81/768). In vitro fertilized embryos that developed to the 2-cell stage were again cryopreserved by vitrification, and after warming they were transferred into recipient females. Subsequently, six viable offspring were delivered, and all were confirmed to be transgenic mice. These results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm.
  • Activation with ethanol improves embryo development of ICSI-derived oocytes by regulation of kinetics of MPF activity, N Fujinami, Y Hosoi, H Kato, K Matsumoto, K Saeki, A Iritani, JOURNAL OF REPRODUCTION AND DEVELOPMENT, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 50(2), 171 - 178, Apr. 2004 , Refereed
    Summary:Developmental potential of bovine embryos that are not artificially activated after intracytoplasmic sperm injection (ICSI) is generally very low. In this study, we investigated effects of artificial activation with ethanol on kinetics of maturation promoting factor (MPF) activity (p34(cdc2) kinase activity) and development of bovine oocytes following ICSI. Treatment of oocytes with ethanol at 4 h after ICSI improved their first cleavage and further preimplantation development (51% vs. 13%, 14% vs. 4%: treatment with vs. without ethanol, respectively). MPF activity of oocytes was lowered until at least 2 h after ICSI. In oocytes without activation after ICSI, MPF activity temporarily elevated at 6 h after ICSI, whereas this phenomena was not observed in the oocytes treated with ethanol. Furthermore, MPF activity was elevated 20 h after ICSI in oocytes activated with ethanol, whereas this elevation of MPF activity was not shown in oocytes without activation. These results indicate that the stimulus of sperm was sufficient to lower MPF activity of oocytes following ICSI, and moreover the activation treatment of bovine oocytes with ethanol after ICSI served to maintain the low levels of MPF activity until the next cell cycle started.

Conference Activities & Talks

  • FGF4 modulates the development of mouse somatic nuclear transfer embryos,   2009 12
  • Expression of the components of chromatin remodeling factor SWR1 complex in mouse somatic nuclear transfer eggs,   2009 12
  • Dynamics of Arp family proteins and components of chromatin remodeling complexes in in vitro differentiation of mouse embryonic stem cells,   2009 12
  • Reconstitution of seminiferous tubules in xenoectopic transplantation with bovine testicular cells, 42nd Annual Meeting for the Japanese Society of Developmental Biologists,   2009 05 , 42nd Annual Meeting for the Japanese Society of Developmental Biologists
  • Expression of Bcrp1 mRNA isoforms during in vitro differentiation of mouse embryonic stem cells, 41st Annual Meeting for the Japanese Society of Developmental Biologists,   2008 05 , 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Expression of transcription factor Cdx2 and Oct3/4 in mouse somatic cell nuclear transfer embryos.,   2007 05
  • Identification and characterization of the 5’-flanking region of three mouse maternal genes (Histone H100, Nucleoplasmin2, and Zygote attest1): Transcriptional activity in mouse oocytes., 33rd Annual Meeting of Ingternational Embryo Transfer Society,   2007 01 , 33rd Annual Meeting of Ingternational Embryo Transfer Society
  • Effects of trichostatin A on development of bovine somatic cell nuclear transfer embryos., The 33rd Annual Conference of the International Embryo Transfer Society,   2007 01 , The 33rd Annual Conference of the International Embryo Transfer Society
  • Differentiation of hepatocyte-like cells from mouse embryonic stem cells in a monolayer culture system., The 33rd Annual Conference of the International Embryo Transfer Society,   2007 01 , The 33rd Annual Conference of the International Embryo Transfer Society
  • Effect of aging on amounts of DNA methyltransferase mRNA in mouse spermatozoa., The 33rd Annual Conference of the International Embryo Transfer Society,   2007 01 , The 33rd Annual Conference of the International Embryo Transfer Society
  • Analysis of global DNA methylation in bovine spermatozoa., The 32nd Annual Conference of the Intenational Embryo Transfer Society,   2007 , The 32nd Annual Conference of the Intenational Embryo Transfer Society
  • DNA methylation profiles of upstream elements of Oct3/4 gene in in vitro fertilization (IVF) and somatic cell nuclear-transferred (SCNT) embryos., The 33rd Annual Conference of the International Embryo Transfer Society,   2007 01 , The 33rd Annual Conference of the International Embryo Transfer Society
  • In vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells using monolayer-culture system., The 3rd Annual Conference of Asian Reproductive Biotechnology Society,   2006 11 , The 3rd Annual Conference of Asian Reproductive Biotechnology Society
  • Bovine somatic cell nuclear transfer embryos: effects of the gene expression ability and DNA methylation on their development to the blastocyst stage., 39th Annual Meeting of the Society for the Study of Reproduction,   2006 08 , 39th Annual Meeting of the Society for the Study of Reproduction
  • Expression of maternal gene promoter driven expression vectors in mouse oocytes and fertilized eggs., 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,   2006 06 , 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Relation of spatial gene expression patterns in bovine embryos reconstructed with somatic cells to blastocyst development., 2006 Annual Conference of Int'l Embryo Transfer Soc,   2006 01 , 2006 Annual Conference of Int'l Embryo Transfer Soc
  • In vitro culture of CD9-expressing cells enriched by magnetic cell sorting from testes of cryptorchid adult and pup in mice., The 32nd Annual Conference of the International Embryo Society,   2006 01 , The 32nd Annual Conference of the International Embryo Society
  • Methylation of the 5’-upstream region of the H19 gene in mouse somatic cell, gametes, wild type and androgenetic ES cells,   2005 06
  • Cytological analysis of hepatic gene expression and immunological response of MHC antigens in mouse amniotic epithelial cells.,   2005 01
  • Analysis of frozen mammoth tissue that excavated fron Siberian Permafrost,   2004 10
  • Kinetics of destabilized luc+ gene expression in mouse preimplantaion embryos, The 4th Conference of The Pacific Rim Society for Fertility and Sterility,   2004 03 , The 4th Conference of The Pacific Rim Society for Fertility and Sterility
  • Establishment of ES cell lines from diploid androgenetic embryo for producing germline chimera, The 4th Conference of the Pacific Rim Society for Fertility and Sterility,   2004 03 , The 4th Conference of the Pacific Rim Society for Fertility and Sterility

Works

  • Molecular Bioengineering of Food Animals

Misc

  • Simple vitrification of mouse early embryos derived from somatic cell nuclear transfer, AZUMA RIKA, NAKAYA MASATAKA, INOUE TATSUYA, KAJIMOTO MIZUKI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 近畿大学先端技術総合研究所紀要, 20, 20, 19, 29,   2015 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201502250815336688
    Summary:近畿大学先端技術総合研究所紀要編集委員会[要旨] これまで遺伝資源の保存技術の構築のために, 様々な動物種の培養細胞を樹立し遺伝資源の確保と細胞特性の検討を行っている. しかし, 高度な発生工学・生殖工学的技術の習熟には時間を要し, 特に体細胞核移植操作は, 作製した再構築胚の発生率が非常に低率であることから多くの胚を作製しなければならない状況下にある. そこで本実験では, 体細胞核移植(Somatic cell nuclear transfer : SCNT)により作製した初期胚のガラス化保存を実施し, 加温後の正常性を検討した. B6D2F 1マウスへ常法に従い過剰排卵処置後にSCNT を実施した. 続いて発生した胚は簡易ガラス化法によりガラス化保存を行った. 加温後, 形態的に正常と認められた胚は, DAPI 染色および免疫染色(Oct4)による組織学的観察と胚発生を検討した. SCNT により作製し, 生存した卵子を活性化処理後, 2細胞期へ発生した再構築胚は83.2% であり, 一部は胚盤胞期へ発生した(44.8% : 47/105). これら再構築胚をガラス化保存し一部を加温した結果, 加温後の生存胚は70% 以上であり免疫組織化学的解析により形態的な正常性を認めた. 以上の結果から, 作製されたSCNT胚のガラス化保存による遺伝資源の保存とそれらの胚を用いた移植核の内部構造構築に関する基礎的検討が可能であることが示唆された. [Abstract] Somatic cell nuclear transfer(SCNT) has been used in various research namely investigation into cellular features for establishing of genetic resourses technology from wild animals. However, it requires a high level of developmental engineering and reproduction technology which take time to learn as well as many successfully reconstructed oocytes and embryos. In this experiment, we examined the cryopreservation by simple vitrification of the mouse early embryos produced by SCNT. After warming a part of cloned embryos, morphologically normal embryos were obtained at more than 70%. And they were morphologically normal by the investigation with immunohistochemical analysis, which detected Oct4, puluripotency facter in inner cell mass. In conclusion, these results suggested that the genetic resources preservation were possible by simple vitrification of cloned mouse embryos. Furthermore, fundamental study on the internal structure of the cell nucleus can be made possible using the cloned mouse embryos.
  • マウスGV期由来卵子を用いた体外成熟後の卵子母性制御機構に関する基礎検討, SAKITA MEGUMI, KAMEI MIKU, NAKAGAWA TAKAO, NISHIMURA MANAMI, NAKAIE MASATAKA, KOBAYASHI SHINTARO, HIGASHI RIKA, MITANI TASUKU, KATO HIROMI, KISHI MASAO, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 日本実験動物学会総会講演要旨集, 61st, 245,   2014 05 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402283057517607
  • 体外成熟培地へのアミノ酸誘導体添加がマウス未成熟卵子由来初期胚の発生に与える効果, KAMEI MIKU, SAKITA MEGUMI, NAKAGAWA TAKAO, NAKAIE MASATAKA, NISHIMURA MANAMI, HIGASHI RIKA, KOBAYASHI SHINTARO, MITANI TASUKU, KATO HIROMI, KISHI MASAO, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 日本実験動物学会総会講演要旨集, 61st, 246,   2014 05 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402287287945670
  • Analysis of the conservation state of 15,000 years old mammoth soft tissue by the amplification of Mc1r gene, KONDO KENJI, YOSHIZAKI TAKUMI, KATO HIROMI, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 19, 55, 61,   2014 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402275522962580
  • A genetic resource conservation technology by establishing cultured fibroblast cells derived from two wild mice, ANZAI MASAYUKI, MURAI HITOSHI, MIYASHITA MINORU, KISHI MASAO, NAKAYA MASATAKA, NISHIMURA MANAMI, SUGIMOTO NAO, MATSUZAKI HIKARU, AZUMA RIKA, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, 近畿大学先端技術総合研究所紀要, 19, 13, 23,   2014 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402278247301003
    Summary:近畿大学先端技術総合研究所紀要編集委員会[要旨]本実験では、野生種であるアカネズミおよびカヤネズミから少量の組織サンプルを回収し効率よく安定したDNA を回収するために、バロサイクラー(Baro Cycler)を用いてDNA 抽出とその相同性について検索を行なった。さらに、両マウスの尾部から採取した組織より線維芽細胞を樹立し、染色体解析および樹立した細胞の遺伝資源保存を検討した。その結果、尾部組織および樹立線維芽細胞それぞれはシークエンス後のBLAST 検索によってデータベース上にて高い相同性を示した。また、核型解析では、それぞれの核型は高い正常性を示した。さらに、それら体細胞を用いて、電気融合法による異属間体細胞核移植を実施したところ、一部の再構築卵子は2 細胞期胚へと発生することを認めた。以上の結果から、今回樹立した野生マウス由来線維芽細胞に異常は少なく、それら細胞の遺伝子資源の保存が可能であることが示された。 [Abstract] In this study, to improve the DNA extraction efficiencies for a small amount of tissue from two wild mice, we have done a search for DNA homology with Baro Cycler. In addition, fibroblasts were also established from those mice tails. Karyotype analyses were performed on the fibroblasts, and those cells were frozen for the preservation. As a result, the frozen-thawed fibroblast cells have a high number of normal karyotype. Fibroblasts derived from organization and tail confirmed the homology sequence after BLAST search. Furthermore, these somatic cells performed somatic cell nuclear transfer(SCNT)by electrofusion method. These results of reconstructed oocytes several developed to 2-cell stage embryos. In conclusion, abnormalities in this wild mouse derived fibroblast cells were low, and thereforeconservation of genetic resources was possible in these somatic cells.
  • Analysis of the conservation state of 15,000 years old mammoth soft tissue by the amplification of Mclr gene, 近藤 健二, 吉崎 匠, 加藤 博己, 入谷 明, Memoirs of Institute of Advanced Technology, Kinki University, 19, 55, 61,   2014 03 , http://ci.nii.ac.jp/naid/120005423588
    Summary:近畿大学先端技術総合研究所紀要編集委員会[要旨] 本実験では、約15,000 年前のマンモス皮膚、筋肉および骨髄の各組織の核DNA の保存状態を解析する事を目的に、核DNA に存在するMelanocortin type 1 receptor(Mc1r)遺伝子をPCR によって増幅し、その増幅の成否を検討した。その結果、骨髄組織由来Total DNA をPCR のTemplate とした区においてのみ、目的とする221bp のDNA 断片が増幅されたことから、マンモス骨髄組織に存在するDNA の保存状態は皮膚および筋肉組織に比べて良好であることが示された。しかし、骨髄組織由来Total DNA をTemplateとた約500bp 程度のDNA 断片を増幅するMc1r 遺伝子増幅プライマーを用いてPCR を行った結果、目的とするDNA 断片は増幅されなかった。以上の結果より、このマンモス組織においては、骨髄中の核DNAは他の皮膚または筋肉組織中の核DNA よりも保存状態が良いが、DNA の断片化が進行していることが示された。 [Abstract] In this study, to analyze the conservation state of 15,000 years old mammoth skin, muscle and bone marrow tissue by PCR, we tried to amplify the DNA fragment of Melanocortin type 1 receptor( Mc1r)gene which exists in the cell nuclear. In results, only in the reaction in which the total DNA from the mammoth's bone marrow was used as the template of PCR, the 221bp DNA fragment was amplified. However, when we tried to amplify longer DNA fragment( about 500bp in length) of Mc1r gene, no DNAfragment was amplified by PCR even when the total DNA from the mammoth's bone marrow was used as the template of PCR. From these results, it was suggested that the conservation state of the bone marrow is better than the skin and muscle tissue, however, the fragmentation of nuclear DNA was also heavily progressed in the bone marrow tissue.
  • マウス始原生殖細胞におけるGSEタンパク質の発現解析, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIHARA TAKUSHI, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, UCHIHORI SHO, AMANO TOMOKO, NAGAI KOHEI, KISHIGAMI SATOSHI, KATO HIROKI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, J Reprod Dev, 59, Suppl Japanese Issue, J114,   2013 08 20 , 10.14882/jrds.106.0.P-26.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302273473633087
  • Reconstitution of seminiferous tubules by xenoectopic transplantation of juvenile bovine testicular cells into the subcutis of immunodeficient mice, KITA SHOTA, MORIKI KOSHIRO, TANIGUCHI SHUNJI, ANZAI MASAYUKI, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, MITANI TASUKU, 近畿大学先端技術総合研究所紀要, 18, 29, 38,   2013 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302213741649592
    Summary:[要旨] 1994年、精原幹細胞の精細管移植によりドナー由来の精子を作製できる移植技術が開発された。さらに、2007年には幼若ブタ精巣細胞を免疫不全マウスの皮下へ移植することにより、精子形成の誘導に成功したことが報告された。そこで本研究では、絶滅危惧動物などでの精子形成分化誘導モデルの確立を目的に、ウシ精巣細胞を用いた移植実験を行った。マウス新生子(生後7日以内)、ウシ新生子(生後7 日以内)およびウシ幼若(3~5ヶ月齢)精巣細胞をマトリゲルマトリックスと混合し、免疫不全マウスの皮下に移植することで、精巣様構造の再構築と精子形成の誘導を試みた。移植後、1週~ 44週に組織学的解析を行った。さらに、生殖細胞の生存・増殖・分化について、抗VASA 抗体を用いた免疫組織化学的解析を行った。マウス新生子精巣細胞の移植では、移植後10週以降に再構築精細管および減数分裂に至る精子細胞がみられ、精子形成誘導を可能にする機能的な精細管の構築が示された。一方、新生子ならびに幼若ウシ精巣細胞の移植では、移植後10 週から管構造の再建がみられたものの、免疫組織化学的解析において再構築された精細管様構造の基底膜上に配置される生殖細胞を認めることはできなかった。以上の結果から、異種異所移植法によりウシにおいても精細管様構造が再構築されたことから, 今後移植法の改良によりウシ精子形成の誘導が可能となることが期待される。 [Abstract] Male germ cell transplantation induces donor cell-derived spermatogenesis in the testis of recipient mice. However, this technique is still available only to mice and rats. Recently, xenoectopic transplantation of testicular cells into the subcutis of immunodeficient mice has been developed as a novel technology to rebuild seminiferous tubules and to produce functional sperm. In order to develop an alternative technology for transgenesis and induction of spermatogenesis in domestic animals, we examinedxenoectopic transplantation of bovine testicular cells. Testicular cells from neonatal(within 7 days)and juvenile(3-5 months)bovine and neonatal(within 7 days)mouse testes were prepared at the concentration of 1-5 x 10^7/ml(mouse)or 1 x 10^8/ml(bovine)and then mixed with equal volume of BD Matrigel^<TM> Basement Membrane Matrix(Matrigel). The testicular cell/Matrigel suspensions were grafted into the subcutis of castrated immunodeficient male mice(BALB/c Slc-nu/nu). Morphogenesis of the grafts was examined between 1 and 44 weeks after transplantation. To examine the growth and differentiation of donor germ cells, the expression of VASA, which is expressed from gonocytes to early meiotic stages in mammalian male germ cells, was examined by immunohistochemistry. Grafts derived from mouse testicular cells regenerated complete functional seminiferous tubules 20 weeks after transplantation in which haploid spermatids were generated in the lumen. Initiation of reconstruction of seminiferous tubules from bovine testicular cells was also observed 10 weeks after transplantation. However, immunohistochemical analysis demonstrated that bovine germ cells failed to colonize along the basement membrane of reconstructed seminiferous tubules and to proliferate thereafter. Although further investigation for suitable conditions of bovine germ cell colonization and survival in the reconstructed seminiferous tubules is needed, xenoectopic transplantation would make possible to induce bovine spermatogenesis and be usefull for animal sciences, conservation of wild animals and male infertility.
  • 近交系マウス由来未成熟卵子を用いた生殖補助技術により作出した各卵子母性制御機構に関する検討, ANZAI MASAYUKI, NISHIMURA MANAMI, AZUMA RIKA, NAKAIE MASATAKA, KAMEI MIKU, SAKITA MEGUMI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 1P-0669 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402206788995589
  • マウスES細胞においてヒストンH2A.Zはヒストン脱アセチル化酵素阻害剤により選択的に除去される, KEIMOTO TETSUYA, NAKAIE MASATAKA, KATO HIROMI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, HARADA MASAHIKO, MITANI TASUKU, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 1P-0328 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402295249672230
  • マウス始原生殖細胞で発現するGSEタンパク質の能動的DNA脱メチル化への関与, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIHARA TAKUSHI, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, UCHIBORI SHO, AMANO TOMOKO, NAGAI KOHEI, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, 日本分子生物学会年会プログラム・要旨集(Web), 36th, 2P-0252 (WEB ONLY),   2013 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201402296885395420
  • Large-scale proteomic analysis of Japanese Black cattle: Part II. Assessment of gender and genetic background effects, IKEGAMI HARUKA, KOBAYASHI NAOHIKO, MATSUHASHI TAMAKO, TAKEMOTO ATSUSHI, YOSHIHIRO TAKUYA, INOUE ETSUKO, KATO RIE, KATO HIROMI, TAGUCHI YOSHITOMO, AMANO TOMOKO, MORIMOTO KOICHI, NAKAGAWA MASARU, IRITANI AKIRA, MATSUMOTO KAZUYA, 日本畜産学会報, 83, 3, 281, 290,   2012 08 25 , 10.2508/chikusan.83.281, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201202296118357071
  • Actual status of paleontological subjects including mammoth, 加藤 博己, 入谷 明, Memoirs of Institute of Advanced Technology, Kinki University, 0, 17, 31, 38,   2012 03 , http://ci.nii.ac.jp/naid/40019293608
    Summary:Airticles[要旨] 我々は今回、2012年2月27日から3月2日まで、ロシア連邦サハ共和国ヤクーツク市において、サハ共和国科学アカデミーの主催によって行われた「古生物学サンプルの研究」セミナーに参加した。このセミナーは、昨年までにサハ共和国で発掘された、ユカマンモス、ユカギルバイソンおよびユカギルウマの遺物からのサンプリングと研究討議を行うものである。行われたセミナーの内容とサンプルについて概説する。 [Abstract] This time, we attended the seminar entitled "Research of Paleontological Objects" which was hosted by The Academy of Science of the Republic of Sakha(Yakutia)in Yakutsk, Republic of Sakha(Yakutia)on February 27^<th>, 2012 to March 2^<nd>, 2012. The objectives of this seminar were sampling from and discussion about the research on "Yuka mammoth", "Yukagir bison" and "Yukagir horse" those excavated from the Siberian permafrost until 2011. In this review, we report the outline of the seminar and samples.
  • マウスGSEの始原生殖細胞における発現プロファイルは,性差がある, MORITA KOTARO, HATANAKA YUKI, SHIMIZU NATSUMI, NISHIKAWA SATOSHI, NISHIHARA TAKUJI, KATO RIE, TAKEMOTO ATSUSHI, HIGUCHI CHIKA, AMANO TOMOKO, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, 日本分子生物学会年会プログラム・要旨集(Web), 35th, 1P-0126 (WEB ONLY),   2012 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201302220460117423
  • Gender identification of birds, KATO HIROMI, MIYASHITA MINORU, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 16, 1, 6,   2011 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102213324534753
    Summary:[要旨] 鳥類は雌へテロ型の性染色体構成をとり、哺乳類とは異なった性決定機構を持っている。鳥類の産業的な利用や飼育下の繁殖において、各個体の雌雄判別は非常に大きな課題である。本文では過去から現在に至るまで、実施されてきた種々の鳥類の雌雄鑑別法について総説する。 [Abstract] Birds have a different sex chromosome system compared to mammals. Female birds have heterogametic ZW sex chromosomes and male birds have homogametic ZZ sex chromosomes. The gender identification of each bird has huge effect on the industrial utilization of birds and the reproduction program of wild or rare species in captivity. In this text, gender identification methods developed so far were reviewed.
  • Establishment of in situ hybridization technique with mouse single ovum that uses QuantiGene ViewRNA, NISHIMURA MANAMI, KONDO KENJI, KIGA KEITA, HIGASHI KASUMI, TABE HIROKAZU, NAKAGAWA TAKAO, KATO HIROMI, HOSOI YOSHIHIKO, ANZAI MASAYUKI, 近畿大学先端技術総合研究所紀要, 16, 35, 42,   2011 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102245186560976
  • Development of effective cell nuclei recovery method from cryopreserved cattle bone marrow tissues, KONDO KENJI, KATO HIROMI, ANZAI MASAYUKI, MITANI TASUKU, SUZUKI ATSUO, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 16, 59, 65,   2011 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102297547999587
    Summary:[要約] 本研究では、マンモス骨髄組織からの効率的な細胞核の回収方法を開発するために、組織の状態が類似したウシ凍結骨髄組織からの効率的な細胞核の回収方法の開発を試みた。さらに、回収方法の有用性を検討するために、回収したウシ凍結骨髄組織由来細胞核をマウス除核未受精卵子に注入し、回収された核の生物学的特性の有無を検討した。 初めに、凍結保存されたウシ骨髄組織からの効率的な細胞核の回収方法の開発を試みた。ウシ骨髄組織から細胞核を回収する際に、Collagenase 処理を行う事によって、細胞核の回収量が有意に向上した。次に、回収したウシ凍結骨髄組織由来ドナー細胞核をマウス除核未受精卵子へ注入し、再構築卵子の早期染色体凝集および前核様構造物の確認を行った。その結果、早期染色体凝集ならびに前核様構造物の形成は観察されなかったものの、細胞核の注入直後に観察されたPropidium iodide による強い赤色蛍光が、細胞核注入後7 時間ではほぼ消失していた事から、生物学的特性は保持されていると考えられた。以上の結果より、生物学的特性を保持した状態の細胞核を大量に回収できる新しい細胞核の回収方法を開発する事に成功した。 [Abstract] We tried to develop the effective nuclei recovery method from cryopreserved cattle bone marrow tissues for the development of effective nuclei recovery method from mammoth bone marrow tissues. In addition, we studied whether its cell nuclei still kept their biological characteristics by injecting recovered nuclei into mouse enucleated matured oocytes. First, we tried to develop the effective nuclei recovery method from cryopreserved cattle bone marrow tissues. The amount of recovered cell nuclei was significantly increased by using collagenase treatment. Subsequently, we tried to inject recovered cell nuclei into mouse enucleated matured oocytes and checked for premature chromatin condensation and pronuclear-like structure. As a result, there was no oocyte with premature chromatin condensation and pronuclear-like structure, but because the highly-red fluorescence which was observed shortly after injection nearly disappeared after 7 hours, their cell nuclei were shown to keep biological characteristics. Based on these results, our method was shown to be effective in mass recovery of cell nuclei that kept their biological characteristics.
  • 卵子および一細胞期胚におけるタンパク質のアセチル化に関する解析, MATSUBARA KEIGO, LEE AH REUM, KAMATA YU, MAGOTANI MASATERU, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, IRITANI AKIRA, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, J Reprod Dev, 56, Suppl Japanese Issue, J64,   2010 08 20 , 10.14882/jrds.103.0.12.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002250746875672
  • Studies on Nuclear Transfer Using Cells from Mouse Bone Marrow Tissue Frozen without Cryoprotectant, NAKAO AKIYOSHI, KATO HIROMI, ANZAI MASAYUKI, MITANI TASUKU, SUZUKI ATSUO, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 15, 9, 16,   2010 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002227496195776
  • 卵子活性化に伴うチューブリンのアセチル化の解析, MATSUBARA KEIGO, LEE AH REUM, KAMATA YU, MAGOTANI MASATERU, OKUYAMA NORIYUKI, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, IRITANI AKIRA, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, 生化学, ROMBUNNO.1P-0889,   2010 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002252260882912
  • マウス胚性幹細胞におけるABCトランスポーター・Bcrp1mRNAアイソフォームAの転写制御領域の解析, HIRANO DAIKI, KAWAMURA HIROKO, NISHIMURA YUI, SAEKI KEITA, ANZAI MASAYUKI, KATO HIROMI, TAGUCHI YOSHITOMO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, ROMBUNNO.4P-0843,   2010 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002274122783214
  • プロテオミクスによるマウス着床前期胚の発生関連タンパク質の解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SHIN SHOKYOKU, NISHIKAWA KEI, RI KOKIN, HATANAKA YUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 日本畜産学会大会講演要旨, 111th, 78,   2009 09 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902221157582893
  • マウス初期胚におけるDD2‐2遺伝子は2OSプロテアソームの形成に関与している, SHIN SHOKYOKU, TOKORO MIKIKO, NISHIKAWA KEI, RI KOKIN, HATANAKA YUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 日本畜産学会大会講演要旨, 111th, 78,   2009 09 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223084547469
  • 若齢ウシ精巣細胞を用いたヌードマウス皮下への異種異所移植による精子形成誘導の試み, MORIKI KOSHIRO, KITA SHOTA, FUJIMOTO YUKI, TANIGUCHI TOSHIHITO, ANZAI MASAYUKI, KATO HIROKI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, MITANI TASUKU, IRITANI AKIRA, J Reprod Dev, 55, Supplement, J96,   2009 08 25 , 10.14882/jrds.102.0.220.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902208863387070
  • FGF4がマウス体細胞核移植胚の発生に与える効果, MORITA MASAHIRO, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, MITANI TAKUMI, IRIYA AKIRA, J Reprod Dev, 55, Supplement, J84,   2009 08 25 , 10.14882/jrds.102.0.134.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902229379782638
  • プロテオミクスを用いたマウス初期胚における発生関連タンパク質の解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SHIN SHOKYOKU, NISHIKAWA SATOSHI, RI KOKIN, HATANAKA YUKI, AMANO TOMOKO, MITANI TADASHI, KATO HIROKI, ANZAI MASAYUKI, KISHIGAMI TETSUJI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 55, Supplement, J142,   2009 08 25 , 10.14882/jrds.102.0.1075.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902240214150169
  • マウス初期胚におけるDD2‐2遺伝子は20Sプロテアソームの形成に関与している, SHIN SHOKYOKU, NOOI MIKIKO, NISHIKAWA SATOSHI, RI KOKIN, HATANAKA YUKI, AMANO TOMOKO, MITANI TADASHI, KATO HIROKI, ANZAI MASAYUKI, KISHIGAMI TETSUSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 55, Supplement, J74,   2009 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902243111735869
  • マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響, KAWAMURA HIROKO, KAWAI TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROKI, HOSOI YOSHIHIKO, MITANI TADASHI, IRIYA AKIRA, J Reprod Dev, 55, Supplement, J79,   2009 08 25 , 10.14882/jrds.102.0.123.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902291185683946
  • 胎子及び幼若マウスにおける生殖腺特異的発現遺伝子GSEの発現解析, HATANAKA YUKI, SATO MANABU, NORO MIKIKO, SHIN SHOKYOKU, NISHIKAWA SATOSHI, RI KOKIN, AMANO TOMOKO, MITANI TADASHI, KATO HIROKI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 55, Supplement, J120,   2009 08 25 , 10.14882/jrds.102.0.1030.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902296541630056
  • マウス初期胚におけるプロテオーム解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SATO MANABU, SHIN SEUNG-WOOK, NISHIKAWA SATOSHI, HATANAKA YUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, J Mamm Ova Res, 26, 2, S77,   2009 04 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902200078039940
  • Expression of the proteins involved in the formation of nuclear and chromatin structures in mouse somatic cell nuclear transfer eggs, NISHIYAMA YUI, MORITA MASAHIRO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, MITANI TASUKU, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 14, 21, 30,   2009 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902244704742450
  • ウシ栄養膜細胞によるリクローン胚の初期発生および胚移植, TANIGUCHI SHUNJI, FUKUHARA JUNKO, KISHI MASAO, IWAMOTO DAISAKU, MATSUI TAKANORI, KISHIGAMI SATOSHI, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 31, 1, 58,   2009 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902299849608913
  • マウスES細胞の分化過程における未分化維持機構においてABCトランスポーターBcrp1の影響, KAWAMURA HIROKO, KAWAI TOMOKO, MORIKI KOSHIRO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 191,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002212646226678
  • マウスES細胞の分化過程におけるArpファミリーならびにクロマチンリモデリング複合体構成因子の動態, MORIKI KOSHIRO, NISHIYAMA YUI, KAWAMURA HIROKO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, HARATA MASAHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 190,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002222527720821
  • FGF4はマウス体細胞核移植胚の初期発生を促進する, MORITA MASAHIRO, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 日本分子生物学会年会講演要旨集, 32nd, Vol.1, 179,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002238117619833
  • トランスジェニックマウスを用いた母性遺伝子プロモーター解析, HATANAKA YUKI, NAKANO AKIHIRO, TSUNEMOTO KAZUNOBU, ANZAI MASAYUKI, SATO MANABU, TOKORO MIKIKO, SHIN SHOKYOKU, WATANABE TATSUYA, SHIMIZU NATSUMI, AMANO TOMOKO, MITANI TADASHI, KATO HIROKI, KISHIGAMI TETSUSHI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 54, Supplement, J75,   2008 08 25 , 10.14882/jrds.101.0.213.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902211106455794
  • マウス初期胚でDD2‐2遺伝子は20Sプロテアソームの形成に関与している, SHIN SHOKYOKU, TOKORO MIKIKO, SATO MANABU, NISHIKAWA KEI, AMANO TOMOKO, KISHIGAMI TETSUSHI, ANZAI MASAYUKI, MITANI TADASHI, KATO HIROKI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 54, Supplement, J76,   2008 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902262073322380
  • マウス着床前期胚における大規模プロテオーム解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SATO MANABU, SHIN SHOKYOKU, NISHIKAWA SAYOSHI, SHIMIZU NATSUMI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI TETSUSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 54, Supplement, J95,   2008 08 25 , 10.14882/jrds.101.0.507.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902282060083280
  • トランスジェニックマウスを用いた母性遺伝子プロモーター解析, NAKANO AKIHIRO, TSUNEMOTO KAZUNOBU, ANZAI MASAYUKI, SATO MANABU, TOKORO MIKIKO, SHIN SEUNGWOOK, HATANAKA YUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, MATSUMOTO KAZUYA, J Mamm Ova Res, 25, 2, S94,   2008 04 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902292880177683
  • Induction of three germ layer cells from parthenogenetic or androgenetic embryo origin embryonic stem cells in vitro, ONODERA YUTA, TERAMURA TAKESHI, TAKEHARA TOSHIYUKI, MURAKAMI HIDEKI, OZAWA MADOKA, TAKEUCHI HIROKI, ANZAI MASAYUKI, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, SAGAWA NORIMASA, HOSOI YOSHIHIKO, 近畿大学先端技術総合研究所紀要, 13, 9, 19,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902201121629493
  • Expression of Bcrp1 mRNA isoforms of mouse embryonic stem cells, KAWAMURA HIROKO, AMIMOTO NAOKI, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROMI, IRITANI AKIRA, MITANI TASUKU, 近畿大学先端技術総合研究所紀要, 13, 29, 40,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223230443387
  • Gene expression activity of the LTR of murine IAP-like bovine retrotransposon, KATO HIROMI, KAWANO YOSHIHIRO, TAKAHASHI YOSHIE, KODA TOSHIO, KISHIMOTO MANABU, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 13, 61, 70,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902249081535353
  • Growth and fertility of cloned mouse by somatic cell nuclear transfer and microsatellite analysis of its genetic background, MORITA MASAHIRO, NISHIWAKI MEGUMI, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 近畿大学先端技術総合研究所紀要, 13, 51, 59,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902256412145964
  • Expression of stem and germ cell markers in juvenile bovine testis and its primary culture, MORIKI KOSHIRO, TAZUHARA YOHEI, TANIGUCHI SHUNJI, ANZAI MASAYUKI, KATO HIROMI, SAEKI KAZUHIRO, IRITANI AKIRA, MITANI TASUKU, 近畿大学先端技術総合研究所紀要, 13, 41, 50,   2008 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902268757894682
  • In vitroにおける単為発生/雄性発生胚由来ES細胞からの三胚葉由来機能細胞の獲得, ONODERA YUTA, TERAMURA TAKESHI, TAKEHARA TOSHIYUKI, FUKUNAGA NAOTO, ITO SHUNSUKE, MURAKAMI HIDEKI, ANZAI MASAYUKI, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, KISHIGAMI TETSUJI, IRITANI AKIRA, SAGAWA NORIMASA, HOSOI YOSHIHIKO, 再生医療, 7, 242,   2008 02 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902221602095710
  • 雌性単為発生胚と雄性単為発生胚由来ES細胞から誘導したインスリン産生細胞の機能性の検討, TAKEUCHI HIROKI, TERAMURA TAKESHI, KISHIGAMI TETSUJI, ANZAI MASAYUKI, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, SAGAWA NORIMASA, HOSOI YOSHIHIKO, 再生医療, 7, 241,   2008 02 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902242585690223
  • 体外受精妊娠成功における精子DNAの重要性:メトホルミン投与による精子DNA断片化指数(DFI)の改善と妊娠の成立, YANAGISAWA FUMIKA, JINNO MASAO, KATO HIROMI, MATSUMOTO KAZUYA, 日本産科婦人科学会雑誌, 60, 2, 510,   2008 02 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902237977507016
  • 過排卵処理したマウス卵巣におけるプロテオーム解析, MATSUMOTO KAZUYA, IKEGAMI HARUKA, NAGAI KOHEI, SONO YOHEI, TOKORO MIKIKO, SHIN SHOKYOKU, MATSUOKA TOSHIKI, MITANI TADASHI, KATO HIROKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本はい移植学雑誌, 30, 1, 55,   2008 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902246872021303
  • 培養ウシ栄養膜細胞の効率的な単離および核移植, TANIGUCHI TOSHIHITO, IWAMOTO TAISAKU, MATSUI TAKANORI, ABE YUKI, KISHIGAMI SATOSHI, KISHI MASAO, KATO HIROKI, MITANI TADASHI, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRITANI AKIRA, SAEKI KAZUHIRO, 日本はい移植学雑誌, 30, 1, 57,   2008 01 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902250660987191
  • マウス着床前期胚における大規模プロテオーム解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SATO MANABU, SHIN SEUNG-WOOK, NISHIKAWA SATOSHI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 4P-0894,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223409224866
  • 若齢ウシ雄性生殖細胞の同定ならびにヌードマウス皮下移植による精子形成誘導の試み, MORIKI KOSHIRO, KITA SHOTA, TANIGUCHI SHUNJI, ANZAI MASAYUKI, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 3P-0886,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902227490032416
  • トリコスタチンA処理によるマウス体細胞核移植胚の着床初期における胚発生に関わる遺伝子発現の解析, MORITA MASAHIRO, NISHIWAKI MEGUMI, ANZAI MASAYUKI, NISHIYAMA YUI, KATO HIROMI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 4P-0895,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902237521150191
  • マウス初期胚で性腺特異的遺伝子DD2‐2が20Sプロテアソームの形成に及ぼす影響, SHIN SEUNG-WOOK, TOKORO MIKIKO, SATO MANABU, NISHIKAWA SATOSHI, AMANO TOMOKO, KISHIGAMI SATOSHI, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 4P-0892,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902285509934742
  • マウスES細胞分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式およびRNAiによるアイソフォームAノックダウンES細胞株樹立の試み, KAWAMURA HIROKO, AMIMOTO NAOKI, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 1P-1076,   2008 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902288909820001
  • Rhophilin‐2遺伝子はマウス受精卵における第1分裂の細胞質分裂に関与している, MATSUOKA TOSHIKI, TOKORO MIKIKO, SHIN SHOKYOKU, AMANO TOMOKO, MITANI TADASHI, KATO HIROKI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, MATSUMOTO KAZUYA, J Reprod Dev, 53, Supplement, J138,   2007 09 25 , 10.14882/jrds.100.0.12048.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902291966233440
  • Development of an examination method of a methylation state of bull sperm DNA, KISHIMOTO MANABU, KODA TOSHIO, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 12, 43, 50,   2007 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902251911739210
  • Expression of transcription factor Cdx2 and Oct3/4 in mouse somatic cell nuclear transfer embryos, NISHIWAKI MEGUMI, MITANI TASUKU, ANZAI MASAYUKI, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 12, 33, 42,   2007 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902256838155819
  • マウス受精卵における分裂機構へのrhophilin‐2 遺伝子の関与, MATSUOKA TOSHIKI, TOKORO MIKIKO, SHIN SERNG-WOOK, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 1P-0457,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902212124018136
  • トランスジェニックマウス(Tgマウス)由来卵子及び着床前胚における母性遺伝子のプロモーター活性, ENDO NORIMASA, TSUNEMOTO KAZUNOBU, ANZAI MASAYUKI, MATSUOKA TOSHIKI, TOKORO MIKIKO, SHIN SEUNGWOOK, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 2P-1309,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902252743397607
  • マウスMII期卵母細胞における網羅的タンパク質発現(プロテオーム)解析, TOKORO MIKIKO, KAWASUMI MIYURI, NAGAI KOHEI, IKEGAMI HARUKA, SHIN SEUNG-WOOK, MATSUOKA TOSHIKI, SATO MANABU, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, ANZAI MASAYUKI, KISHIGAMI SATOSHI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, MATSUMOTO KAZUYA, 生化学, 2P-1307,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902268338533254
  • マウス体細胞核移植胚における栄養外胚葉分化に関する転写因子の発現, NISHIWAKI MEGUMI, ANZAI MASAYUKI, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, MITANI TASUKU, 生化学, 1P-0991,   2007 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902297357245626
  • マウス体細胞核移植胚および体外受精胚におけるOct‐3/4遺伝子転写調節領域のメチル化, KAWASUMI MIYURI, UNNO YUICHI, NISHIWAKI MEGUMI, MATSUMOTO KAZUYA, ANZAI MASAYUKI, MITANI TADASHI, KATO HIROKI, AMANO TOMOKO, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, J Reprod Dev, 52, Supplement, J75,   2006 08 25 , 10.14882/jrds.99.0.62.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902217883336454
  • 母性発現遺伝子(Histone H1oo(H1oo),Nucleoplasmin2(Npm2),Zygote arrest1(Zar1))のプロモーター領域の解析, TSUNEMOTO KAZUNOBU, MATSUMOTO KAZUYA, ANZAI MASAYUKI, AMANO TOMOKO, MITANI TADASHI, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, J Reprod Dev, 52, Supplement, J88,   2006 08 25 , 10.14882/jrds.99.0.87.0, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902253388876180
  • Methylation of the 5'-upstream region of the H19 gene in mouse somatic cell, gametes, wild type and androgenetic ES cells, KATO HIROMI, MURAKAMI HIDEKI, KAWASUMI MIYURI, KUNIEDA TAKANORI, OKUNO MANABU, KISHIMOTO MANABU, SOUMA MANABU, IWAI DAIGO, ANZAI MASAYUKI, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 11, 41, 49,   2006 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902235833075453
  • Localization of the autoimmune regulator (aire) protein in the gonads and embryonic stem cells in mice., MASUDA ATSUHIRO, MITANI TASUKU, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 11, 15, 21,   2006 03 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902263431472050
  • 初期G1期細胞によるウシ再構築はいの遺伝子発現状態と初期発生との関係, IWAMOTO TASAKU, SAEKI KAZUHIRO, KASAMATSU REI, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, KATO HIROKI, TANIGUCHI TOSHIHITO, DETA ATSUSHI, URAKAWA MAMI, AOYAGI TAKAHITO, IRIYA AKIRA, 日本畜産学会大会講演要旨, 106th, 84,   2006 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902227977066855
  • ウシゲノム中に存在するレトロトランスポゾンのLTRに関する研究, KISHIMOTO MANABU, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本畜産学会大会講演要旨, 106th, 68,   2006 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902277717854318
  • Sperm chromatin structure assay法によるウシ精子DNA断片化の解析, KODA TOSHIO, KISHIMOTO MANABU, NOTO KENJI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, KATO HIROMI, IRIYA AKIRA, 日本はい移植学雑誌, 29, 1, 62,   2006 01 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902221633288540
  • ウシ栄養膜細胞由来核移植胚作製のための体外受精胚のバイオプシー手法の検討, TANIGUCHI TOSHIHITO, KAMEYAMA SHINJI, YAMAMOTO MASAKO, HAYASHI NOBORU, TACHIMIZO ATSUHIRO, KASAMATSU REI, IWAMOTO TAISAKU, SAEKI KAZUHIRO, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本はい移植学雑誌, 29, 1, 61,   2006 01 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902260329113063
  • ホウレンソウ由来Δ12脂肪酸不飽和化酵素遺伝子導入マウスにおけるプロテオーム解析, SHINKAI YUSUKE, MATSUMOTO KAZUYA, AMANO TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, SUZUKI IWANE, MURATA NORIO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 540,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902213559909810
  • マウス初期はい特異的発現ベクターの開発, TSUNEMOTO KAZUNOBU, MATSUMOTO KAZUYA, ANZAI MASAYUKI, AMANO TOMOKO, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902216149803763
  • マウス初期はいにおけるrhophilin‐2遺伝子の機能解析, MATSUOKA TOSHIKI, HAYAKUMO MASANORI, SONO YOHEI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902246956838577
  • マウス体細胞核移植はいにおけるOct‐4遺伝子転写調節領域のCpGメチル化, UNNO YUICHI, KAWASUMI MIYURI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会講演要旨集, 28th, 703,   2005 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902268610686626
  • ルシフェラーゼ遺伝子導入細胞によるウシ再構築はいの割球における遺伝子発現とその後の初期発生との関係, IWAMOTO TAISAKU, SAEKI KAZUHIRO, KASAMATSU REI, TAMARI TOMOHIRO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, MITANI TADASHI, KATO HIROKI, TANIGUCHI TOSHIHITO, IRITANI AKIRA, 日本畜産学会大会講演要旨, 105th, 74,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902223942369086
  • マウス初期はいで発現するrhophilin‐2遺伝子の機能解析に関する研究, MATSUOKA TOSHIKI, EN YOHEI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, J Reprod Dev, 51, Supplement, J57,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902234541386451
  • マウス尾部細胞,ES細胞,IVFはい及びNTはいにおけるOct‐3/4遺伝子転写調節領域のCpGメチル化, UMINO YUICHI, KAWASUMI MIYURI, MATSUMOTO KAZUYA, ANZAI MASAYUKI, AMANO TOMOKO, MITANI MASASHI, KATO HIROKI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, J Reprod Dev, 51, Supplement, J65,   2005 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902259427986069
  • The study on the developmental potential of somatic cell nuclear transferred mouse embryos., KAWASUMI MIYURI, KUNIEDA TAKANORI, ANZAI MASAYUKI, MITANI TADASHI, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 10, 57, 61,   2005 03 15 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902219520552360
  • Effect of dimethyl sulfoxide (DMSO) on development of cloned embryos, KUNIEDA TAKANORI, KAWASUMI MIYURI, ANZAI MASAYUKI, KATO HIROMI, MITANI TADASHI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 10, 53, 56,   2005 03 15 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902229580974294
  • Analysis of DNA Methylation Pattern in Mouse Early Embryos by Bisulfite-Sequencing Method, Yuichi Unno, Miyuri Kawasumi, Kazuya Matsumoto, Tomoko Amano, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Hiromi Kato, Kazuya Matsumoto, Masayuki Anzai, Tasuku Mitani, Kazuhiro Saeki, Yoshihiko Hosoi, Akira Iritani, Journal of Mammalian Ova Research, 22, 4, 241, 245,   2005 , 10.1274/jmor.22.241, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902220305375618
    Summary:Recently, with the acetylation of histone and the modification of chromatin structure, the methylation of cytosine residue within CpG dinucleotides in genomic DNA sequence attracts many researchers' attention as one of major epigenetic regulation systems of gene expression. There are several methods (immunofluorescence with 5-methylcytosine specific antibody and methylationsensitive restriction enzyme-PCR method, etc.) to analyze the methylation of cytosine residue. Bisulfitesequencing method, which is one of methods for analyzing the methylation of cytosine residue in genomic DNA sequence, has advantages of high sensitivity and analyzing the methylation of cytosine residue directly. In this note, the detailed procedure of bisulfite-sequencing method for mouse early Preimplantation embryos is described. © 2005, JAPANESE SOCIETY OF OVA RESEARCH. All rights reserved.
  • 植物由来Δ12脂肪酸不飽和化酵素遺伝子導入マウスにおける網羅的遺伝子発現解析, SHINKAI YUSUKE, MATSUMOTO KAZUYA, AMANO TOMOKO, TAGUCHI YOSHITOMO, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, SUZUKI IWANE, MURATA NORIO, 日本分子生物学会年会プログラム・講演要旨集, 27th, 1009,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902205325166641
  • マウス初期はいにおける時計遺伝子群の発現解析, MATSUSHITA AKINORI, AMANO TOMOKO, MATSUMOTO KAZUYA, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 27th, 713,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902278194790384
  • マウス初期はいにおけるはい性遺伝子発現機構へのDNAメチル化及びヒストンアセチル化の関与, YAMAMOTO YUMI, MATSUMOTO KAZUYA, AMANO TOMOKO, KURIHARA TAKASHI, ANZAI MASAYUKI, MITANI TASUKU, KATO HIROMI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, 日本分子生物学会年会プログラム・講演要旨集, 27th, 714,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902279039552902
  • マウス体細胞核移植はいにおける微小管の変化, KAWASUMI MIYURI, ANZAI MASAYUKI, KUNIEDA TAKANORI, KATO HIROMI, MITANI TASUKU, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 27th, 537,   2004 11 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902292351200998
  • マウス初期はいにおける時計遺伝子群の発現解析, MATSUSHITA SATONORI, AMANO TOMOKO, MATSUMOTO KAZUYA, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, J Reprod Dev, 50, Supplement, J57,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902214844920620
  • 植物由来脂肪酸不飽和化酵素を発現させたトランスジェニックマウスにおける遺伝子挿入部位の解析, SHINKAI YUSUKE, YUASA KAZUYUKI, KIRIMOTO SHINJI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, TAGUCHI YOSHITOMO, J Reprod Dev, 50, Supplement, J94,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902253720697103
  • マウス初期はいで発現するrhophilin‐2遺伝子と相互作用する因子の探索, MATSUOKA TOSHIKI, MATSUMOTO KAZUYA, AMANO TOMOKO, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, IRIYA AKIRA, J Reprod Dev, 50, Supplement, J58,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902267653494848
  • マウス初期はいのはい性遺伝子発現機構におけるDNAメチル化及びヒストンアセチル化の関与, YAMAMOTO YUMI, MATSUMOTO KAZUYA, AMANO TOMOKO, KURIHARA TAKASHI, ANZAI MASAYUKI, MITANI MASASHI, KATO HIROKI, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, J Reprod Dev, 50, Supplement, J59,   2004 08 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902283953789853
  • ”Life, inducing from DNA”-Analysis of ancient animal's DNA-,   2004 07
  • ウシ体細胞クローンはいにおける機能的遺伝子の転写調節領域のCpGメチル化, KURIHARA TAKASHI, MATSUMOTO KAZUYA, TOMINAGA KEIICHIRO, ANZAI MASAYUKI, MITANI TADASHI, KATO HIROKI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本畜産学会大会講演要旨, 103rd, 112,   2004 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902229554983226
  • Effects of sperm viability on kinetics of MPF activity of bovine oocytes following ICSI., FUJINAMI NAHOKO, HOSOI YOSHIHIKO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 9, 15, 20,   2004 02 28 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902257803909205
    Summary:生存精子と死滅精子を用いてICSI を行い、精子の生死がその後の胚発生と卵子のMPF 活性の動態に及ぼす影響を検討した。生存精子を用いたICSI 後の卵割率、胚盤胞期胚への発生率はそれぞれ51%、12%で、死滅精子を用いた区に比べ有意に高かった(12%、0%)。生存精子を用いたICSI 後の卵子のMPF 活性は、4 時間目に成熟卵子の値の14%に低下した後、16 時間目まで低値を維持し、24 時間目に次の細胞周期にむけた上昇が認められた。死滅精子を用いたICSI 後のMPF 活性は、ICSI 後4 時間目に成熟卵子の値の45%に低下した後、12 時間目に最低値となり、24 時間目に上昇が見られたが、その値は成熟卵子の26%であった。以上の結果より、生存精子を用いたICSI 後の卵子のMPF 活性は、死滅精子を用いた場合より低値になる時間が早く、精子の生死がICSI 後のMPF 活性の低下に関係していると考えられた。死滅精子を用いた場合は、卵子のMPF 活性が低値を示す時間が短く、次の細胞周期に向けてのMPF 活性の上昇も不十分であるため、ICSI 後の胚の発生率が低いと考えられた。ICSI に用いる精子の生死により、ICSI後のMPF 活性の動態は変化し、その後の胚発生の差異に影響することが示唆された。緒 言
  • Cryotopによるウサギ未受精卵の凍結保存とその後の受精能力の検討, SHIBAGAKI MIHO, KATO HIROKI, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, NISHIKAWA YOSHINOBU, NISHIKAWA KIYOSHI, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本受精着床学会雑誌, 20, 1, 137, 138,   2003 03 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902276580751501
  • Effect of the heparin during ovine frozen-thawed spermatozoa culture for sperm capacitation on further embryonic development after in vitro fertilization of ovine oocytes matured in culture., KATO HIROMI, BRAUN J, HOLLERRIEDER J, LEIDL W, IRITANI AKIRA, 近畿大学先端技術総合研究所紀要, 7, 29, 33,   2002 01 31 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902176230755585
  • Molecular Cloning and Sequencing of cDNAs Encoding Rabbit Inter-.ALPHA.-Trypsin Inhibitor Heavy-Chain 2 and 3 Precursors., SUZUKI ATSUO, KATO HIROMI, IRITANI AKIRA, 近畿大学生物理工学研究所紀要, 6, 15, 24,   2001 06 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902137973893715
    Summary:ウサギ肝臓から、インター -α- トリプシンインヒビター(ITI)重鎖2および3前駆体(HC2, HC3)をコードするcDNAを、RT-PCR (reverse-transcription polymerase chain reaction) 法およびRACE (rapid amplifyication of cDNA end) 法を用いて単離し、その塩基配列を決定した。単離したcDNAはそれぞれ947、904残基のアミノ酸翻訳領域を含む 3,122、2,823塩基対であった。HC2のアミノ酸配列をヒト、マウスおよびハムスターのHC2のそれと比較したところ 85、84, 83%の同一性をもっていた。同じような結果はHC3でも得られた。すでに報告したウサギHC1でも同様であった。しかし、同種の鎖間すなわちHC1とHC2、HC1とHC3、HC2とHC3では、40、52, 40%と低い同一性を示した。このことは、重鎖それぞれが独自に進化し、それぞれ独自の機能を持っていることを示唆している。 (英文)Complementary DNA encoding precursors of the two heavy chains (HC2, HC3) of the inter -α- trypsin inhibitor was amplified from rabbit liver total RNA by reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE), cloned and sequenced. The cDNAs spanned astretch of 3,122 and 2,823 nucleotides with open reading frame coding for 947 and 904 amino acid residues respectively. The deduced amino acid sequence of the HC2 precursor was 85,84, and 83% identical with those of the HC2 precursors from man, mouse, and hamster, respectively. The HC3 and HC1, described previously, precursors showed similar degrees of sequence identity with the corresponding human, mouse, and hamster HC precursors. When the intra-species HCs precursors were compared with each other, HC1 to HC2, HC1 to HC3, and HC2 to HC3, however, the deduced amino acid sequence showed only 40, 52, and 40% sequence identity, respectively. These results suggest that HC genes have been evolving independently of each other under purifying selection and each HC subfamily may have unique fuction(s).
  • Production of new-type genetic resources by gene manipulation and the technology development for its application. Research on production of functional foods by introduction of plant and marine organism genes into animals., IRITANI AKIRA, KOMANO TOORU, HOSOI YOSHIHIKO, SAEKI KAZUHIRO, KATO HIROKI, TAGUCHI YOSHITOMO, 遺伝子操作による新型遺伝資源の生産とその応用に関する技術開発 平成8-12年度 近畿大学生物理工学研究所研究成果報告書, 11(1),11-291,   2001 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902127846908384
  • 植物由来不飽和化酵素遺伝子を導入したトランスジェニックマウスの解析, BUSHIMOTO HIROKO, HOSOI YOSHIHIKO, YANO HIROKI, TAGUCHI YOSHIAKI, KATO HIROMI, MIKAMI KOJI, KINOSHITA MIKIRO, MURATA NORIO, IRITANI AKIRA, J Reprod Dev, 45, Supplement, A16,   2000 12 25 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902137757087593
  • Effects of hCG supplement into bovine oocyte maturation medium on oocyte maturation, fertilization and embryonic development., KATO HIROMI, IRITANI AKIRA, 近畿大学生物理工学研究所紀要, 5, 19, 24,   2000 11 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902145911826984
    Summary:継続後誌:近畿大学先端技術総合研究所紀要= Memoirs of Institute of Advanced Technology, Kinki University無血清培地を使用したウシ未成熟卵胞卵子の成熟培養時に、培地へ添加されたhCGのその後の体外受精および胚発生への影響を検討した。卵胞卵子の成熟率、体外受精後の受精率および受精した卵子での雄性前核の形成率は、添加されたhCGの濃度が0IU/mlから100IU/m1へと増加するにつれて上昇した。授精後48時間での2細胞期胚以上への卵割率はhCG1および100IU/ml添加区において、0および0.1IU/ml添加区よりも有意に高い値を示した(P<0.05)。また、授精後9日目での胚盤胞期胚への発育は、添加されるhCGの濃度が高くなるにつれて、8%から22%へと増加した。以上の結果より、無血清培地を用いたウシ卵胞卵子の体外成熟系において、培地へのhCGの添加はその後の体外受精および胚の発生に有効である事が示された。 (英文) The effects of hCG supplement into the serum-free bovine oocyte maturation medium on oocyte maturation, in vitro fertilization (IVF) and further embryonic development after IVF were examined. When the concentration of hCG in oocyte maturation medium was increased from 0IU/ml up to 100IU/ml, the percentage of matured and fertilized oocyte and fertilized oocyte with male pronucleus were also increased hCG dose dependently. The cleavage rates at 48 hours after insemination were significantly higher with oocytes that matured in the medium supplemented 1 or 100 IU/ ml hCG than those with oocytes that matured in the medium supplemented 0 or 0.1 IU/ml hCG (P<0.05). The rate of embryonic development to the blastocyst stage at 9 days after insemination was also increased hCG dose dependently ( 8 to 22%, , 0 IU/ml to 100IU/ml hCG, respectively) . These results suggested that the supplementation of hCG in high concentration into serum-free bovine oocyte maturation medium is effective on further fertilization and embryonic development.
  • The Effect of Inorganic Phosphate in a Culture Medium on Development of Bovine Early Embryos., SAEKI KAZUHIRO, TAKATSUKA AKIMITSU, MATSUMOTO KAZUYA, HOSOI YOSHIHIKO, KATO HIROMI, IRITANI AKIRA, 近畿大学生物理工学研究所紀要, 5, 25, 29,   2000 11 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902146448260123
  • Efficiency of Rabbit Piezo-ICSI(Intracytoplasmic Sperm Injection) compared to Conventional ICSI., SUZUKI CHIGUSA, HOSOI YOSHIHIKO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, 近畿大学生物理工学研究所紀要, 5, 30, 33,   2000 11 30 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902193134900134
  • Effect of seasonal factors on the quality of bovine follicular oocyte., KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, 近畿大学生物理工学研究所紀要, 4, 22, 28,   2000 03 21 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902191091949913
    Summary:継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki Universityウシ未成熟卵胞卵子の性状が年間を通じてどのように変化するのかを、屠場で採集した卵巣より回収した、ウシ卵胞卵子を、体外成熟・体外受精し、さらに授精後48時間目に3細胞期胚以上へ発育したものを結紮したウサギ卵管へ移植し、5日後に回収して、胚盤胞期胚への発育を検討した。また実験は2年間にわたり、各月ごとに2回行い、結果を集計した。卵子の成熟率は各月において差は無かった。受精率は各月間において変動が大きく、明かな傾向は認められなかったが、10月から1月にかけては、58~69%と低下するのが観察され、また、2月から5月にかけては87~91%と回復するのが見られた。授精後48時間での卵割率は、10月から2月にかけては22~36%と低い値を示し、3月から9月にかけては42~51%にまで回復した。ウサギ卵管から回収された胚の胚盤胞期胚への発育率は全体に低い(0~14%)ため、差異を検討することはできなかったが、5月から8月にかけては高い(8~14%)傾向が見られた。 (英文) The seasonal change of the quality of bovine follicular oocytes was examined. Oocytes were collected from slaughterhouse ovaries, matured, fertilized in vitro and cultured in ligated rabbit oviducts for 5 days. These experiments were performed twice per month and monthly for two years. The maturation rates of bovine follicular oocytes did not differ with the month. The rate of fertilization was low in October to January (58-69%) and was high in February to May (87-91%). The cleavage rates at 48 hours after insemination was low in October to February (22-36%) and was high in March to September (42-51%). The rate of embryonic development to blastocyst in rabbit ligated oviducts was high in May to August ( 8 -14%). These findings suggested that the developmental ability of bovine follicular oocytes matured and fertilized in vitro changed seasonally and the developmental ability to blastocyst in the rabbit oviducts was high in summer.
  • 異種円形精子細胞核を注入したハムスター卵母細胞活性化の検討, SUZUKI CHIGUSA, HOSOI YOSHIHIKO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, 日本受精着床学会雑誌, 17, 1, 105, 108,   2000 02 20 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902174837080991
  • 超急速凍結保存した体外受精卵を用いたトランスジェニックマウス作製方法の検討, OTANI SATOSHI, FUJIMOTO HIROKO, YAMADA SHOHEI, MATSUMOTO KAZUYA, KATO HIROMI, SAEKI KAZUHIRO, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本はい移植学雑誌, 22, 1, 37,   2000 01 16 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902129290585680
  • Separation and recovery of spermatids from testis tissue by elutriation., SUZUKI CHIGUSA, HOSOI YOSHIHIKO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, 日本はい移植学雑誌, 22, 1, 1, 4,   2000 01 16 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902173525276540
  • ウシ顕微授精はいの活性化による発生率について, FUJINAMI NAHOKO, HOSOI YOSHIHIKO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, IKU, HOSHIAI KO, 近畿大学生物理工学研究所紀要, 3, 39, 44,   2000 01 12 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902123812642924
  • Differential Displayを使った受精直後のマウス初期はいで発現している遺伝子群の解析, MATSUMOTO KAZUYA, NAKAGAMI KAYOKO, OTAKE SATOSHI, TANAKA HISAYOSHI, HASHIMOTO YUKA, SAEKI KAZUHIRO, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 22nd, 755,   1999 11 22 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902142603019998
  • Injection of rabbit sperm head into oocyte and subsequent embryonic development in vitro., KUSAKA NAOKO, HOSOI YOSHIHIKO, KANEKO TAKEHITO, KATO HIROMI, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, IRITANI AKIRA, 日本はい移植学雑誌, 21, 2, 85, 91,   1999 05 17 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902148653115147
  • 外来遺伝子導入によるウシはい性遺伝子の活性化における転写および翻訳開始時期の検討, KANEKO TAKEHITO, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, HARADA MAYUMI, NOICHI TERUHISA, HOSOI YOSHIHIKO, KATO HIROKI, IRIYA AKIRA, 日本畜産学会大会講演要旨, 95th, 69,   1999 03 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902180395665537
  • 外来遺伝子を注入したウシはいにおける注入遺伝子のはい内での分布と消長, NOICHI TERUHISA, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, KANEKO TAKEHITO, HARADA MAYUMI, HOSOI YOSHIHIKO, KATO HIROKI, IRIYA AKIRA, 日本畜産学会大会講演要旨, 95th, 69,   1999 03 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902186741693699
  • Morphological changes of bovine sperm surfaces during sperm capacitation and acrosome reaction by atomic force microscopy, SAEKI KAZUHIRO, KATO NOBUHIRO, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA, KATO HIROMI, IRITANI AKIRA, Memoirs of the Research Institute of Biology-Oriented Science and Technology, Kinki University, 2, 26, 32,   1999 03 , http://id.ndl.go.jp/bib/4766521
  • Onset of transcription and translation of embryonic gene in early bovine embryos following introduction of exogenous gene into pronuclei, SAEKI Kazuhiro, MATSUMOTO Kazuya, KANEKO Takehito, HARADA Mayumi, NOICHI Teruhisa, HOSOI Yoshihiko, KATO Hiromi, IRITANI Akira, 日本分子生物学会年会プログラム・講演要旨集, 21,   1998 12 01 , http://ci.nii.ac.jp/naid/10002920004
  • Cloning and functional analysis of zygotic gene activated in the mouse 1-cell embryo, MATSUMOTO K, NAKAGAMI K, SATO E, SAEKI K, HOSOI Y, IRITANI A, 日本分子生物学会年会プログラム・講演要旨集, 21,   1998 12 01 , http://ci.nii.ac.jp/naid/10002920005
  • Trascriptional activity of follicular stimulating hormen receptor promoter in ovarian glanulosa cells, SHINOHARA Y, FUJIMOTO H, MATSUMOTO K, SAEKI K, KATO H, HOSOI Y, IRITANI A, 日本分子生物学会年会プログラム・講演要旨集, 21,   1998 12 01 , http://ci.nii.ac.jp/naid/10002920006
  • 卵巣卵胞内か粒膜細胞特異的発現を示すFSH receptor転写領域の解析, SHINOHARA YUKO, FUJIMOTO YUKO, MATSUMOTO KAZUYA, SAEKI KAZUHIRO, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 21st, 605,   1998 11 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902126803230728
  • 外来遺伝子導入によるウシはい性遺伝子の活性化における転写および翻訳時期の検討, SAEKI KAZUHIRO, MATSUMOTO KAZUYA, KANEKO TAKEHITO, HARADA MAYUMI, NOICHI TERUHISA, HOSOI YOSHIHIKO, KATO HIROMI, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 21st, 604,   1998 11 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902129036249875
  • マウス1細胞期はいで発現するはい性由来遺伝子クローニングとその機能解析, MATSUMOTO KAZUYA, NAKAGAMI KAYOKO, SATO EIMEI, SAEKI KAZUHIRO, KATO HIROMI, HOSOI YOSHIHIKO, IRITANI AKIRA, 日本分子生物学会年会プログラム・講演要旨集, 21st, 604,   1998 11 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902169998852257
  • マウス1細胞期はいで発現するはい性由来遺伝子クローニングとその機能解析, MATSUMOTO KAZUYA, SATO HIDEAKI, SAEKI KAZUHIRO, KATO HIROMI, HOSOI YOSHIHIKO, IRIYA AKIRA, 日本繁殖生物学会講演要旨, 91st, 51,   1998 08 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902113561579912
  • Effects of temperature and presence of pyruvic acid in in-vitro maturation of a cattle follicular ovum on external fertilization and embryonic development after culture., 加藤博己, SEIDEL G E JR, 入谷明, 日本畜産学会大会講演要旨, 94th, 164,   1998 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902173337748361
  • Effect of culture supernatant of cattle uterine tubal epithelium cell which forms follicular cell lump in serum free culture on generation of external fertilization egg of cattle., INOUE YUKA, KATO HIROMI, YAMADA MASAYASU, UTSUMI KYOZO, 家畜繁殖学会講演要旨, 86th, 23,   1994 09 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902174019192814
  • Effect of epidermal growth factor(EGF) on in vitro rabbit oocytes maturation and early embryonic development., HOSOI YOSHIHIKO, KATO HIROMI, YAMADA MASAYASU, UTSUMI KYOZO, IRITANI AKIRA, 日本はい移植研究会誌, 1, 2, 133, 138,   1994 06 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902119540955459
  • ラット内細胞塊から分離した継代可能な未分化細胞群の検討, 細井美彦, 岡田久弥, 加藤博己, 山田雅保, 内海恭三, 入谷明, 日本畜産学会大会講演要旨, 88th, 187,   1994 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902102984227565
  • 無血清培養におけるウシ初期はいの発生におよぼすマウス卵管の影響, 加藤博己, 南直治郎, 山田雅保, 内海恭三, 日本畜産学会大会講演要旨, 88th, 194,   1994 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902160109666644
  • Effects of bovine follicular fluid on in vitro maturation of bovine follicular oocytes., TAJIMA JIN'ICHI, KIM K, YAMAJI TAISUKE, CHO H J, MITSUMIZO NARUKI, FUJITA KO, KATO HIROMI, UTSUMI KYOZO, 家畜繁殖技術研究会誌, 15, 2, 41, 48,   1993 05 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902138123549523
  • 無血清培地中で卵胞細胞がウシ未成熟卵胞卵子の成熟と体外受精後のはい発生に及ぼす影響, 加藤博己, 細井美彦, 南直治郎, 山田雅保, 内海恭三, 日本畜産学会大会講演要旨, 87th, 276,   1993 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902134906927630
  • Effect of Electric Re-stimulation on the Development of Nuclear Transplant Bovine Embryos., HOSODA MAKOTO, INOUE YUKA, KATO HIROMI, HOSOI YOSHIHIKO, UTSUMI KYOZO, IRITANI AKIRA, 食肉に関する助成研究調査成果報告書, 10(1991), 18, 21,   1992 12 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902071856046288
  • Effects of Electric Reactivation on the Development of Nuclear-Transplanted Bovine Embryos., HOSODA MAKOTO, INOUE YUKA, KATO HIROMI, HOSOI YOSHIHIKO, YAMADA MASAYASU, UTSUMI KYOZO, 家畜繁殖技術研究会誌, 14, 3, 185, 188,   1992 09 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902096395654925
  • 凍結融解精液を用いたヒツジ体外受精系における精子の受精能獲得に対するHeparinの効果, 加藤博己, HOLLERRIEDER J, BRAUN J, LEIDL W, 入谷明, 日本畜産学会大会講演要旨, 85th, 28,   1992 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902013998056108
  • Effects of granulosa cell, cumulus cell and corona cell on the development of oocytes matured and fertilized in vitro., KATO HIROMI, HOSOI YOSHIHIKO, UTSUMI KYOZO, IRITANI AKIRA, 家畜繁殖技術研究会誌, 14, 1, 20, 28,   1992 01 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902035700558452
  • 体外成熟・受精ウシはい由来分離割球と除核未受精卵との融合はいの発生能について, 二谷匡, 加藤博己, 内海恭三, 入谷明, 日本畜産学会大会講演要旨, 83rd, 13,   1990 03 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902069191290013
  • Cryopreservation procedures for Day 7-8 equine embryos(共著), Equine Veterinary Journal(Supplement), 25, 98, 102,   1997
  • Treatment of equine oocytes with A23187 after intracytoplasmic sperm injection(共著), Equine Veterinary Journal(Supplement), 25, 51, 53,   1997
  • NONSPECIES-SPECIFIC EFFECTS OF MOUSE OVIDUCTS ON THE DEVELOPMENT OF BOVINE IVM/IVF EMBRYOS BY A SERUM-FREE COCULTURE, N MINAMI, H KATO, Y INOUE, M YAMADA, K UTSUMI, A IRITANI, THERIOGENOLOGY, 41, 7, 1435, 1445,   1994 05
    Summary:In Experiment 1, development of bovine embryos derived from in vitro-matured (IVM) and in vitro-fertilized (IVF) oocytes was examined under 4 culture conditions: 1) co-culture with mouse ampullae continuously for 8 d, 2) co-culture with mouse ampullae that were replaced with fresh ampullae at 48-h intervals, 3) co-culture with bovine granulosa cell monolayers, and 4) culture in medium alone. Culture medium consisted of tissue culture medium 199 (TCM-199) supplemented with 1% fetal calf serum (FCS). Inseminated oocytes were transferred to each of the culture treatment 24 h after insemination and were cultured for 8 d. The number of blastocysts per number of cleaved ova obtained after co-culture with mouse ampullae (42.9%) was significantly (P<0.05) higher than that obtained after co-culture with granulosa cell monolayers (28.3%) or culture without cells (4.2%). In Experiment 2, the developmental ability of bovine IVM/IVF embryos co-cultured with mouse ampullae supplemented with or without serum was examined. When serum was excluded from the culture medium, 26.4% (33/125) of the total number of embryos cultured were able to develop to the blastocysts stage using this co-culture system. This value was comparable to that obtained in a serum-supplemented co-culture system (30.7%; 39/125). In addition, the developmental ability of embryos that reached to the 4-cell stage or beyond at 46 to 48 h after insemination was not significantly different when the embryos were co-cultured with mouse ampullae with (38.5 vs 44.6%) or without (37.0 vs 33.8%) serum.
  • ANALYSIS OF SPERM MOTILITY BY COMPUTER-ASSISTED METHODS, WITH SPECIAL CONSIDERATION OF IN-VITRO FERTILIZATION, W LEIDL, H KATO, J HOLLERRIEDER, J BRAUN, MOLECULAR REPRODUCTION AND DEVELOPMENT, 36, 2, 224, 228,   1993 10
  • In vitro fertilization in cattle(共著), Molecular Reproduction and Development, 36, 2, 229, 231,   1993
  • Effects of Electric Reactivation on the Development of Nuclear-Transplanted Bovine Embryos, Japanese Journal of Animal Reproductive Technology, 14, 3, 185, 188,   1992
  • Effects of granulosa cell, cumulus cell and corona cell on the development of oocytes matured and fertilized in vitro, Japanese Journal of Animal Reproductive Technology, 14, 1, 20, 28,   1992
  • EARLY MORPHOLOGICAL EVENTS OF INVITRO FERTILIZED BOVINE OOCYTES WITH FROZEN-THAWED SPERMATOZOA, K SAEKI, H KATO, Y HOSOI, M MIYAKE, K UTSUMI, A IRITANI, THERIOGENOLOGY, 35, 5, 1051, 1058,   1991 05
    Summary:Bovine follicular oocytes were matured and inseminated in vitro with spermatozoa capacitated in vitro. The first evidence of sperm penetration was observed at 3 h after insemination. The penetration rate increased until 5 h, and reached a maximum rate (92%) at 5 h. Decondensation of the sperm head and pronuclear formation were observed 4 h and 7 h after insemination, respectively. Female chromatins of all penetrated oocytes were activated at 3 h, and female pronuclei were formed at 7 h after insemination. Percentages of oocytes with male and female pronuclei at 9 h were 88 and 94%. Polyspermy (4, 7, 19 and 29% at 4, 5, 7 and 9 h after insemination, respectively) and abnormal development of male pronuclei (6 and 7% at 7 and 9 h after insemination, respectively) were also seen.
  • FULL-TERM DEVELOPMENT OF BOVINE FOLLICULAR OOCYTES MATURED IN CULTURE AND FERTILIZED INVITRO, K UTSUMI, H KATO, A IRITANI, THERIOGENOLOGY, 35, 4, 695, 703,   1991 04
    Summary:Follicular oocytes were cultured for 28h in vitro and 91% of the oocytes reached the the second metaphase in culture. The penetration rate after insemination in vitro using frozen-thawed spermatozoa was 81%. After cultivation for 48h in vitro, 18% of the in vitro fertilized oocytes developed to the three- to four-cell stages and 21% of these developed to the six- to eight-cell stages. Following in vivo culture in the rabbit oviduct, 18% of six- to eight-cell and 5% of three- to four-cell embryos developed to the blastocyst stage. To confirm the full developmental competence, 11 blastocysts were transferred to recipient cows, and six (55%) cows became pregnant or delivered calves.

Research Grants & Projects

  • Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(基盤研究(C)), Basic research for resurrection of extinct animals by somatic cell nuclear transfer, The analysis of the conservation state of existing mammoth soft tissue (skin, muscle and bone marrow) by PCR and the development of the method to collect somatic cell nuclei from bone marrow were examined. In results, we succeeded to develop the efficient method to collect somatic cell nuclei from bone marrow. Also we succeeded to have new, well conserved mammoth's skin, muscle, hypodermis and bone marrow from Sakha (Yakutia) Republic. Furthermore, to improve developmental ability of reconstructed embryos, we tried to co-inject mitochondria which derived from cells of same species with donor cell nucleus. However the developmental ability of reconstructed embryos was not improved.
  • Gene Science Research, Production and analysis of transgenic mice that overexpress rabbit P70/P85S6 kinase gene
  • Research for the Future Program, Production and analysis of transgenic mice that overexpress rabbit Sonic Hedgehog gene to increase the number of muscle fiber