FUJINAMI Nahoko

Researcher Information

Degree

• Doctor of Engineering（2004/03 Kindai University）

J-Global ID

• 201401069597673742

Research Interests

• 不妊   卵子・胚   発生・分化   顕微注入   顕微授精   

Research Areas

• Life sciences / Obstetrics and gynecology

Academic & Professional Experience

• 2007/04 - Today  Kindai UniversityFaculty of Medicine助教

Published Papers

• チョコレート嚢胞合併不妊に対する手術の意義

  山本 貴子; 辻 勲; 藤浪 菜穂子; 藤島 理沙; 松村 謙臣

  産婦人科の進歩 「産婦人科の進歩」編集室 72 (4) 463 - 463 0370-8446 2020/10
• Significance of surgery for infertile patients with endometrioma(和訳中)

  Tsuji Isao; Fujishima Risa; Fujinami Nahoko; Matsumura Noriomi

  Acta Medica Kinki University 近畿大学医学会 45 (1) 1 - 11 0386-6092 2020/06
• 不妊治療に苦慮した子宮びまん性平滑筋腫の2例

  藤島 理沙; 辻 勲; 藤浪 菜穂子; 小谷 泰史; 松村 謙臣

  日本生殖医学会雑誌 (一社)日本生殖医学会 64 (4) 453 - 453 1881-0098 2019/10
• Reproductive Outcome of Infertile Patients with Fibroids Based on the Patient and Fibroid Characteristics; Optimal and Personalized Management

  Isao Tsuji; Nahoko Fujinami; Yasushi Kotani; Takako Tobiume; Masato Aoki; Kosuke Murakami; Akiko Kanto; Hisamitsu Takaya; Masayo Ukita; Masao Shimaoka; Hidekatsu Nakai; Ayako Suzuki; Masaki Mandai

  GYNECOLOGIC AND OBSTETRIC INVESTIGATION KARGER 81 (4) 325 - 332 0378-7346 2016 [Refereed]
   

  Aims: To analyze the detailed clinical course of infertile patients with uterine fibroids and to identify optimal and personalized treatment based on the patient or fibroid characteristics. Methods: Retrospective analysis of a case series was performed on 176 infertile patients with fibroids. The patients were classified into different groups according to different treatments (conservative infertility treatment, myomectomy and non-myomectomy surgery). Patient or fibroid characteristics for different groups were analyzed for a possible correlation with the reproductive outcome. Results: The cumulative pregnancy rates by conservative treatment plateaued in 1 year. Myomectomy improved the reproductive outcome in patients who did not conceive with conservative infertility treatments. The most important determinant of the reproductive outcome in patients by conservative treatment prior to surgery was a past patient history of pregnancy. The most important determinant of the reproductive outcome after myomectomy was patient age. Conclusion: Myomectomy should be considered when infertile patients with fibroids do not conceive within 1 year of conservative infertility treatments. The most important determinant of reproductive outcome after myomectomy is patient age. Therefore, for patients younger than 40, the treatment schedule should be carefully considered so that the patients can sufficiently benefit from myomectomy and assisted reproductive technology. (C) 2015 S. Karger AG, Basel
• Pregnancy following ovarian induction in a patient with premature ovarian failure and undetectable serum anti-Mullerian hormone

  Isao Tsuji; Kazumi Ami; Nahoko Fujinami

  JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH WILEY-BLACKWELL 39 (5) 1070 - 1072 1341-8076 2013/05 

  We report the first case, to the best of our knowledge, of successful conception following ovarian induction in a patient with premature ovarian failure and undetectable serum anti-Mullerian hormone. A 34-year-old woman was referred because of ovarian amenorrhea. After endogenous gonadotrophins were normalized by hormone-replacement therapy and gonadotrophin-releasing hormone agonist, ovarian induction was performed using exogenous gonadotrophins. On ovarian induction day 8, one follicle had reached a mean diameter of 19.6mm, the serum estradiol level had increased to 516pg/mL, and human chorionic gonadotrophin (HCG) was injected. On HCG injection day 7, ultrasonography was unable to detect the follicle, and serum progesterone levels had increased to 6.1ng/mL. One month after HCG injection, ultrasonography detected an intrauterine fetus with beating heart. Even with serum anti-Mullerian hormone levels below the threshold of detection, there is a chance for patients with premature ovarian failure.
• The Significance of Laparoscopy in Determining the Optimal Management Plan for Infertile Patients with Suspected Tubal Pathology Revealed by Hysterosalpingography

  Isao Tsuji; Kazumi Ami; Nahoko Fujinami; Hiroshi Hoshiai

  TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE TOHOKU UNIV MEDICAL PRESS 227 (2) 105 - 108 0040-8727 2012/06 

  The fallopian tube has numerous functions, including ovum pick-up, the place of fertilization of the ovum and cleavage of the embryo, and transfer of the embryo to the uterus. Tubal pathology impairs functions of the fallopian tube and reduces fertility. The degree of tubal pathology determines the possibility for fertility. The evaluation of the fallopian tube is necessary to determine the management plan of infertility. Hysterosalpingography (HSG) is often performed as a first line approach to assess tubal patency and the presence of adhesions; however, HSG has limitations in detecting tubal pathology. In the current study, we evaluated the significance of laparoscopy in determining the optimal management plan for infertile patients with suspected tubal pathology revealed by HSG. Between 1997 and 2009, 127 patients with suspected tubal pathology as demonstrated by HSG underwent laparoscopy at Kinki University Hospital, and a retrospective analysis was performed. Of 87 patients with unilateral tubal pathology revealed by HSG, 20 patients (23.0%) were given an indication for assisted reproductive technology (ART), based on the laparoscopic findings. Of 40 patients with bilateral tubal pathology revealed by HSG, 33 patients (82.5%) with bilateral tubal pathology detected by laparoscopy were given a high indication for ART. Laparoscopy enables exact evaluation of the fallopian tube and selection of the optimal management plan in infertile patients with suspected tubal pathology revealed by HSG. Therefore, laparoscopy should be performed in infertile patients with suspected tubal pathology revealed by HSG, as it is of diagnostic importance.
• Body Mass Index別による不妊治療成績の検討

  網 和美; 辻 勲; 藤浪 菜穂子; 宮崎 綾子; 星合 昊

  日本受精着床学会雑誌 29 80 - 82 2012
• 当院不妊外来における血中FSH高値例の検討

  網 和美; 辻 勲; 藤浪 菜穂子; 宮崎 綾子; 星合 昊

  日本受精着床学会雑誌 29 147 - 149 2012
• 子宮筋腫核出術後の妊娠予後規定因子に関する検討

  辻 勲; 網 和美; 宮崎 綾子; 藤浪 菜穂子; 小谷 泰史; 飛梅 孝子; 梅本 雅彦; 塩田 充; 星合 昊

  日本受精着床学会雑誌 29 118 - 121 2012
• 生殖補助医療に移行するまでの配偶者間人工授精の至適回数について

  網 和美; 辻 勲; 宮崎 綾子; 藤浪 菜穂子; 星合 昊

  日本受精着床学会雑誌 28 428 - 432 2011
• Evaluation of Parametrial Spread in Endometrial Carcinoma

  Yoh Watanabe; Takao Satou; Hidekatsu Nakai; Tomomaro Etoh; Kensaku Dote; Nahoko Fujinami; Hiroshi Hoshiai

  OBSTETRICS AND GYNECOLOGY LIPPINCOTT WILLIAMS & WILKINS 116 (5) 1027 - 1034 0029-7844 2010/11 [Refereed]
   

  OBJECTIVE: To evaluate the detailed clinicopathologic characteristics of parametrial spread in uterine endometrial cancer. METHODS: We retrospectively identified 334 individuals with uterine endometrial cancer who had undergone radical hysterectomy between 1988 and 2007. Parametrial spread was determined by histopathological analysis of surgically resected specimens. RESULTS: Twenty-eight (8.4%) individuals had histopathologically confirmed parametrial spread, and lymphatic or blood vessel invasion (22 cases) was the most frequently observed type of parametrial spread; direct invasion to parametrial connective tissue (five cases) or cardinal lymph node metastasis (four cases) were less frequently observed. Parametrial spread occurred not only in individuals with cervical involvement but also in individuals with more than half myometrial invasion, retroperitoneal (pelvic, paraaortic, or both), lymph node metastasis, ovarian metastasis, positive peritoneal cytology results, and lymphovascular space invasion. Twenty-six individuals (92.9%) with parametrial spread showed more than one of these histopathological factors (median number of factors 3, range 1-6); the other two individuals had lymphovascular space invasion alone. In 10 individuals with parametrial spread (35.7%), the condition recurred during the median follow-up period of 49 months, and initial recurrence was observed in the lung in six individuals (60.0%). Although the long-term prognosis for those with parametrial spread was significantly poorer than that of those without parametrial spread, both among all individuals (P<.001) and among individuals with International Federation of Gynecology and Obstetrics stage III (P<.05), multivariate analysis showed that parametrial spread was not an independent prognostic factor for uterine endometrial cancer. CONCLUSION: Parametrial spread cannot be predicted by cervical involvement alone but may be predicted by various lymphovascular space invasion-related histopathologic factors. Further, parametrial spread may not be an independent prognostic factor in individuals with uterine endometrial cancer. (Obstet Gynecol 2010;116:1027-34)
• 子宮筋腫合併不妊症に対する腹腔鏡手術の意義について

  辻 勲; 網 和美; 宮崎 綾子; 藤浪 菜穂子; 小谷 泰史; 飛梅 孝子; 塩田 充; 星合 昊

  産婦人科の実際 59 1563 - 1568 2010
• 卵管性不妊症・子宮性不妊症・子宮内膜症による不妊症

  辻 勲; 金村 和美; 石津 綾子; 藤浪 菜穂子; 星合 昊

  日本産科婦人科学会雑誌 61 67 - 74 2009
• HSG所見正常症例に腹腔鏡手術は必要か？

  辻 勲; 金村 和美; 石津 綾子; 藤浪 菜穂子; 星合 昊

  産婦人科の実際 58 2049 - 2052 2009
• A change in promoter methylation of hMLH1 is a cause of acquired resistance to platinum-based chemotherapy in epithelial ovarian cancer

  Yoh Watanabe; Haruhiko Ueda; Tomomaro Etoh; Eiji Koike; Nahoko Fujinami; Akiyo Mitsuhashi; Hiroshi Hoshiai

  ANTICANCER RESEARCH INT INST ANTICANCER RESEARCH 27 (3B) 1449 - 1452 0250-7005 2007/05 [Refereed]
   

  Background: Acquired resistance to platinum-based chemotherapy (Pt-chemo) is a major problem for improving the prognosis for patients with advanced epithelial ovarian cancer (EOC). However, the molecular mechanism of acquired resistance to Pt-chemo is not well understood. Materials and Methods: hMLH1 promoter methylation (hMLH1 MET) and hMLH1 protein expression was examined in 36 paired samples of primary and secondary resected tumors by methylation-specific polymerase chain reaction (PCR). Results: No primary tumors exhibited hMLH1 MET, while 56.3% of secondary tumors showed hMLH1 MET Moreover, no significant correlation was observed between hMLH1 MET and histological subtype, while hMLH1 MET was significantly greater (p<0.001) in partially responsive secondary tumors compared with no change or progressive disease, and hMLH1 MET also occurred more frequently (p=0.059) in tumors treated with four or more courses of Pt-chemo. Conclusion: A change in hMLH1 MET is a major molecular cause of acquired resistance to Pt-chemo in EOC.
• Primate embryonic stem cells proceed to early gametogenesis in vitro.

  Teramura T; Takehara T; Kawata N; Fujinami N; Mitani T; Takenoshita M; Matsumoto K; Saeki K; Iritani A; Sagawa N; Hosoi Y

  Cloning and stem cells 9 (2) 144 - 156 1536-2302 2007 [Refereed]
  
• Activation with ethanol improves embryo development of ICSI-derived oocytes by regulation of kinetics of MPF activity

  藤浪 菜穂子; 細井 美彦; 加藤; 博己; 松本; 和也; 佐伯; 和弘; 入谷 明

  Journal of Reproduction and Development 50 171 - 178 2005/02
• Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome

  Junko Morita; Yukihiro Terada; Yoshihiko Hosoi; Nahoko Fujinami; Miki Sugimoto; Sou-Ichi Nakamura; Takashi Murakami; Nobuo Yaegashi; Kunihiro Okamura

  Reproductive Medicine and Biology John Wiley and Sons Ltd 4 (2) 169 - 177 1447-0578 2005 

  Aim: In most mammalian fertilization, the sperm introduces the centrosome, which acts as a microtubule organizing center (MTOC) and is essential for pronuclear movement. In rabbit fertilization, biparental centrosomal contribution in microtubule organization has been suggested. Methods: To reveal the function and inheritance of the centrosome during rabbit fertilization, we compared microtubule organization and early embryonal development following intracytoplasmic sperm injection (ICSI) with and without sperm centrosome. Sperm centrosome was removed by sonication, and the isolated sperm head was injected by a Piezo-driven nucromanipulator. Samples were studied by light microscope after immunocytological stain. Results: The sperm aster formation was observed 2-3 h after ICSI with intact sperm. In contrast, microtubules were organized between the male and female pronucleus without a nucleation site in the eggs after ICSI with an isolated sperm head. In the late pronuclear stage following ICSI with an isolated sperm head, microtubule organization was the same as in late pronuclear stage eggs after intact sperm injection. The first mitotic spindle was organized in eggs following ICSI with an isolated sperm head, as observed in eggs following ICSI with an intact sperm. Conclusions: These results indicate that the MTOC is in oocyte cytoplasm during fertilization and fulfils the function when the sperm centrosome is absent.
• Effects of ethanol treatment after intracytoplasmic sperm injection (ICSI) on sperm aster formation and the microtubule organization of bovine oocytes.

  Fujinami N; Hosoi Y; Kato H; Mitani T; Matsumoto K; Saeki K; Hosoi Y; Iritani A

  Reprod. Fertil. Develop. 17 (1) 307  2005/01 [Refereed]
  
• Effects of sperm viability on kinetics of MPF activity of bovine oocytes following ICSI

  藤浪 菜穂子; 細井 美彦; 加藤; 博己; 松本; 和也; 佐伯; 和弘; 入谷 明

  Memoirs of Institute of Advanced Technology, Kinki University 近畿大学先端技術総合研究所 (9) 15 - 20 1346-8693 2004 

  生存精子と死滅精子を用いてICSI を行い、精子の生死がその後の胚発生と卵子のMPF 活性の動態に及ぼす影響を検討した。生存精子を用いたICSI 後の卵割率、胚盤胞期胚への発生率はそれぞれ51％、12％で、死滅精子を用いた区に比べ有意に高かった（12％、0％）。生存精子を用いたICSI 後の卵子のMPF 活性は、4 時間目に成熟卵子の値の14％に低下した後、16 時間目まで低値を維持し、24 時間目に次の細胞周期にむけた上昇が認められた。死滅精子を用いたICSI 後のMPF 活性は、ICSI 後4 時間目に成熟卵子の値の45％に低下した後、12 時間目に最低値となり、24 時間目に上昇が見られたが、その値は成熟卵子の26％であった。以上の結果より、生存精子を用いたICSI 後の卵子のMPF 活性は、死滅精子を用いた場合より低値になる時間が早く、精子の生死がICSI 後のMPF 活性の低下に関係していると考えられた。死滅精子を用いた場合は、卵子のMPF 活性が低値を示す時間が短く、次の細胞周期に向けてのMPF 活性の上昇も不十分であるため、ICSI 後の胚の発生率が低いと考えられた。ICSI に用いる精子の生死により、ICSI後のMPF 活性の動態は変化し、その後の胚発生の差異に影響することが示唆された。緒 言
• Fertilization by Intracytoplasmic Sperm Injection and Subsequent Embryo Development in Vitro to Blastocysts in Japanese Monkey (Macaca Fuscata)

  Yoshihiko Hosoi; Ryuzo Torii; Nahoko Fujinami; Kazuya Matsumoto; Kazuhiro Saeki; Akira Iritani

  Journal of Mammalian Ova Research 20 (1) 34 - 40 1347-5878 2003 

  Intracytoplasmic Sperm Injection (ICSI) has been widely applied for curing human infertility. In this study the developmental potential of Japanese monkey embryos produced by ICSI is reported in a practically relevant system. Oocytes retrieved by laparoscopy from follicles in ovaries of gonadotrophin-stimulated fertile females were fertilized by ICSI using spermatozoa obtained from a fertile male. An additional chemical stimulus was not necessary to achieve oocyte activation with pronuclear formation after ICSI. Successful fertilization was confirmed by extrusion of the second polar body and the presence of both male and female pronuclei at 18-20 h post-ICSI. Some two-cell stage embryos obtained by ICSI were transferred to synchronous recipients and the others were cultured in CMRL medium for 168 h to assess their developmental competence. Oocytes collected laparoscopically from hyper-stimulated monkey ovaries were fertilized by ICSI and completed preimplantation development in vitro, however no pregnancy was confirmed after embryo transfer. This study demonstrates for the first time that the oocytes of the Japanese monkey are able to support advanced embryonic preimplantation development in vitro. It is suggested that the Japanese monkey is an excellent preclinical model for examining and understanding many aspects of ICSI for endangered primates. © 2003, JAPANESE SOCIETY OF OVA RESEARCH. All rights reserved.
• ウシ顕微授精胚の活性化による発生率について

  藤浪 菜穂子; 星合 昊; 細井 美彦; 加藤; 博己; 松本; 和也; 佐伯; 和弘; 入谷 明

  近畿大学生物理工学研究所紀要 近畿大学生物理工学研究所 (3) 39 - 44 1344-414X 2000 

  ウシ卵子を用いた細胞質内精子注入法 (ICSI) では、卵子の活性化がおこりにくく付加の刺激が必要であることが知られている。本実験ではICSI後、カルシウムイオノフォア A23187 およびエタノールによる活性化処理を行い、その発生率の比較を行った。ICSI後の前核期胚への発生率は control区53％(53/105)、A23187処理区64％(65/102)、工タノール処理区54％(56/104)でいずれの区においても差は認められなかった (p＞0.05)。培養2日後、2細胞期以上に発生した胚はcontrol区12％(35/290)、A23187処理区27％(59/217)、工タノール処理区14％(18/126)でありA23187処理区が高かった(p＜0.05)。胚盤胞への発生率はcontrol区0.7％(2/290)、A23187処理区6％(14/217)、エタノール処理区0.8％(1/126)でありA23187処理区が高かった(p＜0.05)。 （英文） Activation of bovine oocytes used in preparation for intracyioplasmic sperm injection (ICSI) has been investigated with combinations of calcium ionophore A23187 and ethanol. Effects were evaluated by assessment of development in vitro. Oocytes matured in vitro and injected of sperm were exposed to : 10μM calcium ionophore A23187 (Groups I ), 7 ％ ethanol in PBS (Groups 2 ), or no treatment as a control (Group 3 ). Pronuclear formation rates of these three groups were 64％ (65/102), 54％ (56/104), 53％ (53/105), respectively. There was no significant difference among these groups (p＞0.05). At two days after culture, cleavage rates of these three groups were 27％ (59/217), 14％ (18/126), 12％ (35/290). Cleavage rates at 2 days post ICSI of these three groups were 27％ (59/217), 14％ (18/126), 12％ (35/290), respectively. Groups I is significantly higher than the others. (p<0.05)Blastulation rates of these groups were 6 ％ (14/217), 0.8％ ( 1 /126), 0.7 ％ ( 2 /290) ,respectively. It was also that Groups I was significantly higher than the others. (P <0.05)Thus, activation of bovine oocytes by calcium ionophore A 23187 after ICSI increase aproportion of development compared to ethanol-activated or non-activated oocytes. It issuggested that activation by calcium ionophore A 23187 is suitable for bovine oocytes ICSI.継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University

Books etc

• 産婦人科の世界, 生殖医療への提言－顕微授精による受精プロセス

  細井 美彦; 藤浪 菜穂子 (Joint work)2004

Conference Activities & Talks

• ESTABILSHMENT OF CYNOMOLGUS MONKEY EMBRYONIC STEM CELL LINES AND CONFIRMATION OF THE POSSIBILITY FOR GERMINAL COMPETENCY.  [Not invited]

  寺村岳士; 藤浪 菜穂子

  The International Embryo Transfer Society  2006/01  Orlando, Florida  The International Embryo Transfer Society
  
• ESTABLISHMENT OF CYNOMOLGUS MONKEY EMBRYONIC STEM CELL LINES AND CONFIRMATION OF POSSIBILITY FOR GERMINAL COMPETENCY.  [Not invited]

  寺村岳士; 藤浪 菜穂子

  The 52nd NIBB conference ~Reproductive Strategies~  2006/01  岡崎  The 52nd NIBB conference ~Reproductive Strategies~
  
• ウサギ凍結乾燥精子による顕微授精(ICSI)胚への活性化処理の検討  [Not invited]

  原 加瑞子; 藤浪 菜穂子

  第23回日本受精着床学会  2005/08  大阪  第23回日本受精着床学会
  
• EGFとエストラジオールがウサギ卵母細胞の体外成熟に及ぼす影響  [Not invited]

  是兼 真子; 藤浪 菜穂子

  第23回日本受精着床学会  2005/08  大阪  第23回日本受精着床学会
  
• ヒト組換えFSHを用いたカニクイザルの反復過剰排卵誘起と回収卵子の発生能の検討  [Not invited]

  藤浪 菜穂子; 星合 昊

  第23回日本受精着床学会  2005/08  大阪  第23回日本受精着床学会
  
• Effect of ethanol treatment after intracytoplasmic sperm injection (ICSI) on sperm aster formation and the microtubule organization of bovine oocytes  [Not invited]

  藤浪 菜穂子; Hosoi Y Kato; H; Mitani; T; Matsumoto; K Saeki; K; Iritani A

  The International Embryo Transfer Society  2005/01  Copenhagen, Denmark  The International Embryo Transfer Society
  
• カニクイザルの反復過剰排卵法と回収卵子の発生能の検討  [Not invited]

  藤浪 菜穂子; 竹之下 誠; 加藤 博己; 三谷; 匡; 松本; 和也; 佐伯; 和弘; 細井; 美彦; 入谷 明

  第22回日本受精着床学会  2004/09  旭川  第22回日本受精着床学会
  
• 超音波診断によるカニクイザルにおける主席卵胞直径と回収卵子の成熟率の関係  [Not invited]

  大田 聖; 藤浪 菜穂子; 竹之下 誠; 矢持 隆之; 國枝; 孝典; 安齋; 政幸; 松本; 和也; 佐伯; 和弘; 細井; 美彦; 入谷 明

  第51回日本実験動物学会  2004/05  長崎  第51回日本実験動物学会
  
• Correlation between follicle size and quality of oocytes from superovulated cynomolgus monkey  [Not invited]

  Takenoshita M; 藤浪 菜穂子; Ohta S; Yamochi T; Kunieda; T Anzai M; Matsumoto; K; Saeki; K Hosoi Y; Iritani A

  The International Embryo Transfer Society  2004/01  Portland, Oregon  The International Embryo Transfer Society
  
• ウシ顕微授精後のエタノール処理が卵子のMPF活性に及ぼす影響  [Not invited]

  藤浪 菜穂子; 細井 美彦; 加藤; 博己; 松本; 和也; 佐伯; 和弘; 入谷 明; 高瀬 規久也

  第21回日本受精着床学会  2003/10  東京品川  第21回日本受精着床学会
  
• Effect of ethanol treatment on MPF activity of bovine oocytes after intracytoplasmic sperm injection (ICSI)  [Not invited]

  藤浪 菜穂子; Hosoi Y Kato; H; Matsumoto; K Saeki; K; Iritani A

  第2回21世紀国際COEシンポジウム  2003/09  泉佐野（関空）  第2回21世紀国際COEシンポジウム
  
• ウサギ受精時の微小管形成に関する精子中心体の役割  [Not invited]

  森田順子; 藤浪 菜穂子

  第44回日本哺乳動物卵子学会  2003/05  東京  第44回日本哺乳動物卵子学会
  
• サル未成熟卵子への成熟卵子細胞質注入による体外成熟誘起の検討  [Not invited]

  北田 建作; 藤浪 菜穂子; 加藤 博己; 佐伯; 和弘; 松本; 和也; 細井; 美彦; 入谷 明; 竹之下 誠; 鳥居 隆三

  第20回日本受精着床学会  2002/10  岐阜  第20回日本受精着床学会
  
• ウシ顕微授精胚のMPF活性  [Not invited]

  藤浪 菜穂子; 加藤 博己; 松本; 和也; 佐伯; 和弘; 細井; 美彦; 入谷 明

  第8回日本胚移植学会  2001/08  山形  第8回日本胚移植学会
  
• ウシ顕微授精胚の発生に及ぼす活性化の影響  [Not invited]

  細井 美彦; 藤浪 菜穂子; 加藤 博己; 松本 和也; 佐伯 和弘; 入谷 明

  第 8 回日本胚移植研究会 （山形）  2001/08  第 8 回日本胚移植研究会 （山形）
   

  ウシ顕微授精を行った後の卵子内 MPF 活性を測定し、 顕微授精後の胚発生と MPF 活性の関係について検討した。
• First successful birth of the cynomolgus monkey (Macaca fascicularis) by intracytoplasmic sperm injection and embryo transfer (ICSI-ET)  [Not invited]

  Torii R; 藤浪 菜穂子; Hosoi Y; Iritani A; Takenoshita Y

  第48回日本実験動物学会  2001/05  横浜  第48回日本実験動物学会
  
• Establishment of the primate embryonic stem cell lines from blastocysts produced by intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) using the Japanese monkey and the cynomolgus monkey  [Not invited]

  Torii R; 藤浪 菜穂子; Hosoi H; Iritani A; Tada T; Suemori; H; Nakatsuji N

  The International Embryo Transfer Society  2001  Omaha, Nebraska, USA  The International Embryo Transfer Society
  
• Development of the cynomolgus monkey oocytes following intracytoplasmic sperm injection  [Not invited]

  藤浪 菜穂子; Hosoi Y; Saeki K; Matsumoto; K; Iritani A; Torii R; Takenoshita Y

  The International Embryo Transfer Society  2001  Omaha, Nebraska, USA  The International Embryo Transfer Society
  
• ニホンザルの室内人工繁殖法(2)-顕微授精・胚移植法の検討-  [Not invited]

  藤浪 菜穂子; 鳥居 隆三; 細井 美彦; 入

  第16回日本霊長類学会大会  2000/07  名古屋  第16回日本霊長類学会大会
  

MISC

• 当院における男性妊孕性温存療法の現状と問題点

  宮川 知保; 村上 幸祐; 佐藤 華子; 山本 貴子; 藤島 理沙; 貫戸 明子; 藤浪 菜穂子  日本がん・生殖医療学会誌  5-  (1)  131  -131  2022/01
• 近畿大学病院での女性患者の妊孕性温存療法の実際

  村上 幸祐; 佐藤 華子; 山本 貴子; 藤島 理沙; 宮川 知保; 貫戸 明子; 藤浪 菜穂子  日本がん・生殖医療学会誌  5-  (1)  134  -134  2022/01
• 排卵誘発ならびに妊娠に成功したPOI(primary ovarian insufficiency)の1例

  藤島 理沙; 辻 勲; 藤浪 菜穂子; 青木 稚人; 村上 幸祐; 高矢 寿光; 浮田 真沙世; 小谷 泰史; 島岡 昌生; 飛梅 孝子; 中井 英勝; 鈴木 彩子; 万代 昌紀  産婦人科の進歩  67-  (2)  234  -234  2015/05
• 当院不妊外来における血中FSH高値例の検討

  網 和美; 辻 勲; 藤浪 菜穂子  産婦人科の実際  61-  (2)  259  -261  2012/02
• [Case Reports] Successful pregnancy foilowing gamete intrafallopian transfer in a patient with a transverse vaginal septum: case report

  Ami Kazumi; Tsuji Isao; Watanabe Yo; Fujinami Nahoko; Miyazaki Ayako; Hoshiai Hiroshi  Acta Medica Kinki University  35-  (2)  129  -131  2010/12
• Analysis of the sex chromosome in human sperm prepared by swim-up method with fluorescence in situ hybridization

  TSUJI Isao; WATANABE Yoh; AMI Kazumi; MIYAZAKI Ayako; FUJINAMI Nahoko; HOSHIAI Hiroshi  Cytometry Research  20-  (2)  1  -4  2010/09
• The Importance of Endoscopy in Infertile Patients with Myomas

  TSUJI ISAO; AMI KAZUMI; MIYAZAKI AYAKO; FUJINAMI NAHOKO; KOTANI YASUSHI; TOBIUME TAKAKO; UMEMOTO MASAHIKO; SHIOTA MITSURU; HOSHIAI HIROSHI  日本受精着床学会雑誌  27-  (1)  308  -312  2010/03
• (4)子宮内膜症による不妊症(1)女性不妊症,4.不妊症,E.婦人科疾患の診断・治療・管理,研修コーナー)

  辻 勲; 金村 和美; 石津 綾子; 藤浪 菜穂子; 星合 昊  日本産科婦人科學會雜誌  61-  (3)  "N  -71"-"N-74"  2009
• (3)子宮性不妊(1)女性不妊症,4.不妊症,E.婦人科疾患の診断・治療・管理,研修コーナー)

  辻 勲; 金村 和美; 石津 綾子; 藤浪 菜穂子; 星合 昊  日本産科婦人科學會雜誌  61-  (3)  "N  -69"-"N-71"  2009
• (2)卵管性不妊症(1)女性不妊症,4.不妊症,E.婦人科疾患の診断・治療・管理,研修コーナー)

  辻 勲; 金村 和美; 石津 綾子; 藤浪 菜穂子; 星合 昊  日本産科婦人科學會雜誌  61-  (3)  "N  -67"-"N-69"  2009
• ウシ顕微授精後のエタノール処理が卵子のMPF活性に及ぼす影響

  藤浪 菜穂子; 細井 美彦; 加藤 博己; ほか3名  The Journal of reproduction and development  50-  (2)  171  -178  2004/04
• The role of sperm centrosome for microtubule organization in rabbit fertilization

  MORITA Junko; TERADA Yukihiro; HOSOI Yashihiko; FUJINAMI Nahoko; NAKAMURA Sou-ichi; YAEGASHI Nobuo; OKAMURA Kunihiko  Journal of mammalian ova research = 日本哺乳動物卵子学会誌  20-  (2)  S55  2003/08
• ニホンザルの顕微鏡授精法による受精と胚盤胞までの体外発生

  細井 美彦; 鳥居 隆三; 藤浪 菜穂子; ほか3名  Journal of mammalian ova research = 日本哺乳動物卵子学会誌  20-  (1)  34  -45  2003/04
• ウシ顕微授精胚の発生に及ぼす活性化の影響 体外受精と顕微授精におけるMPF活性の比較

  藤浪 菜穂子; 加藤 博己; 松本 和也; 佐伯 和弘; 細井 美彦; 入谷 明  日本胚移植学雑誌  24-  (1)  39  -39  2002/01

Research Grants & Projects

• 未成熟卵子の成熟誘起方法の検討-ヒト型モデルとしてのカニクイザルを用いて-

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2005 -2006 

  Author : 藤浪 菜穂子

   

  カニクイザル卵子の入手時期は冬期がピークであるため、比較的入手が容易であるウサギ未成熟卵子(GV期)を使用して成熟誘起を試みた。昨年度はウサギGV期卵子の細胞質にP34^/cyclinB, Protein Kinaseまたはcdk1/cyclinBの注入を行ったが、MII期卵子の獲得には至らなかった。そこで、ウサギのGV期卵子の卵核胞(GV)にMAP kinaseの注入を施行した。その後、体外培養を行った結果、コントロール区では0%(0/20)であったが、注入区では23%(9/39)がMI期、23%(9/39)がMII期へと進行した。同様の方法を用いて、カニクイザルにおける未成熟卵子の成熟誘起を行った結果、コントロール区では0%(0/21)、注入区では75%(15/20)がMI期、15%(3120)がMII期へと進行した。これにより、カニクイザルにおいてGV期卵子のGVへのMAP kinase注入は、減数分裂を再開させる効果があることが示された。 また、得られた力ニクイザルMII期卵子の発生能を検討するため、卵子細胞質内精子注入(ICSI)を施行した。しかし、卵割胚の確認には至らなかった。MAP kinaseの注入による卵子の成熟率は低率であり、成熟を誘起させた卵子は形態的にも異常が多く、そのため質の良い成熟卵子を獲得することが今後の課題である。加えて、Mos/MAPKの活性化を阻害してもGVBDが誘起されるか等を調べ、カニクイザルの卵成熟におけるMPF形成機構についても検討する予定である。

Other link

researchmap

https://researchmap.jp/nami745