KINDAI UNIVERSITY


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IKEDA Hiroko

Profile

FacultyDepartment of Life Science
PositionAssistant Professor
DegreePh.D. in Medicine
Commentator Guidehttps://www.kindai.ac.jp/meikan/2459-ikeda-hiroko.html
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Last Updated :2020/09/02

Education and Career

Education

  •   2006 04  - 2010 03 , Kindai University, Faculty of Science and Engineering
  •   2010 04  - 2012 03 , Gunma University Graduate School of Medicine
  •   2012 04  - 2017 03 , Gunma University Graduate School of Medicine

Academic & Professional Experience

  •   2020 04 ,  - 現在, Assistant professor, Faculty of Science and Engineering, Department of Life Science, Kindai University
  •   2017 04 ,  - 2020 03 , Postdoctoral researcher, International Open Laboratory (Dr. Held lab, Harvard Medical School / MGH), Gunma University Initiative for Advanced Research

Research Activities

Research Areas

  • Environmental science/Agricultural science, Environmental effects of radiation
  • Life sciences, Radiology

Research Interests

  • Cell biology, Bystander effect, Radiation biology

Published Papers

  • Increased chromosome aberrations in cells exposed simultaneously to simulated microgravity and radiation., Hada M, Ikeda H, Rhone JR, Beitman AJ, Plante I, Souda H, Yoshida Y, Held KD, Fujiwara K, Saganti PB, Takahashi A, Int. J. Mol. Sci., Int. J. Mol. Sci., 20(1), 43, 2019 , Refereed
  • Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity, Ikeda H, Muratani M, Hidema J, Hada M, Fujiwara K, Souda H, Yoshida Y, Takahashi A, Int. J. Mol. Sci., Int. J. Mol. Sci., 20(19), 4791, 2019 , Refereed
  • Performance evaluation of new devices to synchronize heavy-ion or X-ray irradiation and simulated microgravity for space radiation research ~ Improvement of a control stage ~., Takahashi A, Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Nikawa T, Yoshida Y, Kanai T, The 31st International Symposium on Space Technology and Science (ISTS), proceeding (Theses ID: 2017-p-09), The 31st International Symposium on Space Technology and Science (ISTS), proceeding (Theses ID: 2017-p-09), 2018 , Refereed
  • A new system for three-dimensional clinostat synchronized X-irradiation with a high-speed shutter for space radiation research., Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Nikawa T, Yoshida Y, Kanai T, Takahashi A, Biol. Sci. Space, Biol. Sci. Space, 30, 8 - 16, 2016 , Refereed
  • Development and performance evaluation of a three-dimensional clinostat synchronized heavy-ion irradiation system, Hiroko Ikeda, Hikaru Souda, Anggraeini Puspitasari, Kathryn D. Held, Jun Hidema, Takeshi Nikawa, Yukari Yoshida, Tatsuaki Kanai, Akihisa Takahashi, Life Sciences in Space Research, Life Sciences in Space Research, 12, 51 - 60, Feb. 01 2017 , Refereed
    Summary:Outer space is an environment characterized by microgravity and space radiation, including high-energy charged particles. Astronauts are constantly exposed to both microgravity and radiation during long-term stays in space. However, many aspects of the biological effects of combined microgravity and space radiation remain unclear. We developed a new three-dimensional (3D) clinostat synchronized heavy-ion irradiation system for use in ground-based studies of the combined exposures. Our new system uses a particle accelerator and a respiratory gating system from heavy-ion radiotherapy to irradiate samples being rotated in the 3D clinostat with carbon-ion beams only when the samples are in the horizontal position. A Peltier module and special sample holder were loaded on a static stage (standing condition) and the 3D clinostat (rotation condition) to maintain a suitable temperature under atmospheric conditions. The performance of the new device was investigated with normal human fibroblasts 1BR-hTERT in a disposable closed cell culture chamber. Live imaging revealed that cellular adhesion and growth were almost the same for the standing control sample and rotation sample over 48 h. Dose flatness and symmetry were judged according to the relative density of Gafchromic films along the X-axis and Y-axis of the positions of the irradiated sample to confirm irradiation accuracy. Doses calculated using the carbon-ion calibration curve were almost the same for standing and rotation conditions, with the difference being less than 5% at 1 Gy carbon-ion irradiation. Our new device can accurately synchronize carbon-ion irradiation and simulated microgravity while maintaining the temperature under atmospheric conditions at ground level.
  • The bystander cell-killing effect mediated by nitric oxide in normal human fibroblasts varies with irradiation dose but not with radiation quality, Yuichiro Yokota, Tomoo Funayama, Yasuko Mutou-Yoshihara, Hiroko Ikeda, Yasuhiko Kobayashi, INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 91(5), 383 - 388, May 2015 , Refereed
    Summary:Purpose : To investigate the dependence of the bystander cell-killing effect on radiation dose and quality, and to elucidate related molecular mechanisms. Materials and methods : Normal human fibroblast WI-38 cells were irradiated with 0.125 - 2 Gy of gamma-rays or carbon ions and were co-cultured with non-irradiated cells. Survival rates of bystander cells were investigated using the colony formation assays, and nitrite concentrations in the medium were measured using the modified Saltzman method. Results : Survival rates of bystander cells decreased with doses of gamma-rays and carbon ions of <= 0.5 Gy. Treatment of the specific nitric oxide (NO) radical scavenger prevented reductions in survival rates of bystander cells. Moreover, nitrite concentrations increased with doses of less than 0.25 Gy (gamma-rays) and 1 Gy (carbon ions). The dose responses of increased nitrite concentrations as well as survival reduction were similar between gamma-rays and carbon ions. In addition, negative relationships were observed between survival rates and nitrite concentrations. Conclusion : The bystander cell-killing effect mediated by NO radicals in normal human fi broblasts depends on irradiation doses of up to 0.5 Gy, but not on radiation quality. NO radical production appears to be an important determinant of gamma-ray-and carbon-ion-induced bystander effects.
  • Gap junction communication and the propagation of bystander effects induced by microbeam irradiation in human fibroblast cultures: The impact of radiation quality, Narongchai Autsavapromporn, Masao Suzuki, Tomoo Funayama, Noriko Usami, Ianik Plante, Yuichiro Yokota, Yasuko Mutou, Hiroko Ikeda, Katsumi Kobayashi, Yasuhiko Kobayashi, Yukio Uchihori, Tom K. Hei, Edouard I. Azzam, Takeshi Murakami, Radiation Research, Radiation Research, 180(4), 367 - 375, Oct. 2013 , Refereed
    Summary:Understanding the mechanisms underlying the bystander effects of low doses/low fluences of low- or high-linear energy transfer (LET) radiation is relevant to radiotherapy and radiation protection. Here, we investigated the role of gap-junction intercellular communication (GJIC) in the propagation of stressful effects in confluent normal human fibroblast cultures wherein only 0.036-0.144% of cells in the population were traversed by primary radiation tracks. Confluent cells were exposed to graded doses from monochromatic 5.35 keV X ray (LET ∼6 keV/μm), 18.3 MeV/u carbon ion (LET ∼103 keV/μm), 13 MeV/u neon ion (LET ∼380 keV/μm) or 11.5 MeV/u argon ion (LET ∼1,260 keV/μm) microbeams in the presence or absence of 18-α-glycyrrhetinic acid (AGA), an inhibitor of GJIC. After 4 h incubation at 37°C, the cells were subcultured and assayed for micronucleus (MN) formation. Micronuclei were induced in a greater fraction of cells than expected based on the fraction of cells targeted by primary radiation, and the effect occurred in a dose-dependent manner with any of the radiation sources. Interestingly, MN formation for the heavy-ion microbeam irradiation in the absence of AGA was higher than in its presence at high mean absorbed doses. In contrast, there were no significant differences in cell cultures exposed to X-ray microbeam irradiation in presence or absence of AGA. This showed that the inhibition of GJIC depressed the enhancement of MN formation in bystander cells from cultures exposed to high-LET radiation but not low-LET radiation. Bystander cells recipient of growth medium harvested from 5.35 keV X-irradiated cultures experienced stress manifested in the form of excess micronucleus formation. Together, the results support the involvement of both junctional communication and secreted factor(s) in the propagation of radiation-induced stress to bystander cells. They highlight the important role of radiation quality and dose in the observed effects. © 2013 by Radiation Research Society.
  • No mutations of lysophosphatidic acid receptor genes in lung adenocarcinomas induced by N-Nitrosobis(2-hydroxypropyl)amine in rats, Naoko Wakabayashi, Megumu Tsujino, Masaki Tajiri, Midori Taki, Ayumi Koshino, Hiroko Ikeda, Nobuyuki Fukushima, Toshifumi Tsujiuchi, Journal of Toxicologic Pathology, Journal of Toxicologic Pathology, 23(1), 63 - 66, Apr. 2010 , Refereed
    Summary:Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats.

Conference Activities & Talks

  • The synergistic effect of simulated microgravity and charged particle radiation on gene expression in human fibroblasts, Ikeda H, Muratani M, Puspitasari A, Held KD, Kawamura H, Hidema J, Yoshida Y, Takahashi A, COSPAR2018, 42th ASSEMBLY,   2018 07
  • Comprehensive gene expression analysis of human fibroblasts using new device to synchronize carbon-ion irradiation and simulated microgravity, Ikeda H, Prairie View A&M University (CRI/RaISE Science seminar),   2018 01 , 招待有り
  • Development of 3D clinostat synchronized irradiation systems for simulating space conditions, Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Hada M, Saganti P, Yoshida Y, Kanai T, Takahashi A, 2018 NASA Human Research Program Investigators’ Workshop,   2018 01
  • Importance of the combined effects of radiation and simulated microgravity for space biosciences, Ikeda H, Puspitasari A, Held KD, Yoshida Y, Takahashi A, 21st IAA Humans in Space Symposium,   2017 11
  • RNAseq profiling to analyze the combined effects on radiation under simulated microgravity for space biosciences, Takahashi A, Ikeda H, Muratani M, Puspitasari A, Held KD, Hidema J, Yoshida Y, 63rd Annual RRS Meeting,   2017 10
  • Performance evaluation of new devices to synchronize X-ray or heavy-ion irradiation and simulated microgravity for space radiation research, Takahashi A, Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Nikawa T, Yoshida Y, Kanai T, The 31st International Symposium on Space Technology and Science,   2017 06
  • New devices to synchronize irradiation and simulated µG for space radiation research, Takahashi A, Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Nikawa T, Yoshida Y, Kanai T, The 5th International Symposium on Space Radiation and Particle Radiotherapy, including the 8th International Workshop on Space Research Radiation (IWSRR) and the 17th Ion Beams in Biology and Medicine (IBIBAM),   2017 05
  • Gene expression profile changes of human fibroblasts using device to synchronize carbon-ion irradiation and simulated microgravity, Ikeda H, Muratani M, Puspitasari A, Held KD, Hidema J, Yoshida Y, Takahashi A, The 5th International Symposium on Space Radiation and Particle Radiotherapy, including the 8th International Workshop on Space Research Radiation (IWSRR) and the 17th Ion Beams in Biology and Medicine (IBIBAM),   2017 05
  • Comprehensive gene expression analysis of human fibroblasts after combined exposure to microgravity and heavy-ions for simulating space conditions, Ikeda H, Muratani M, Puspitasari A, Held KD, Hidema J, Yoshida Y, Takahashi A, The 7th International Society of Radiation Neurobiology Conference,   2017 02
  • Effects of oxygen concentration and radiation quality on radiation-induced DNA damage, Tominaga S, Okada H, Ikeda H, Yoshida Y, Takahashi A, The 7th International Society of Radiation Neurobiology Conference,   2017 02
  • Development of new devices that analyze the combined effects of radiation and microgravity: 2nd report, Takahashi A, Ikeda H, Souda H, Puspitasari A, Held KD, Hidema J, Nikawa T, Yoshida Y, Kanai T, The 7th International Society of Radiation Neurobiology Conference,   2017 02
  • Development of 3D-clinostat synchronized heavy-ion irradiation system, Ikeda H, Hidema J, Nikawa T, Yoshida Y, Souda H, Kanai T, Takahashi A, 11th Asian Microgravity Symposium,   2016 10
  • Evaluation of survival curves for solid tumor cells after large doses of carbon ion beams and X-rays, Yoshida Y, Ando K, Koike S, Yako T, Ikeda H, Uzawa A, Matsumoto T, Isono M, Kanai T, Nakano T, 15th International Congress of Radiation Research,   2015 05
  • Bystander cell-killing effect mediated by nitric oxide in nor mal human fibroblasts depends on irradiation dose but not on radiation quality, Yokota Y, Funayama T, Ikeda H, Mutoh Yoshihara Y, Suzuki M, Sakashita T, Kobayashi Y, 15th International Congress of Radiation Research,   2015 05
  • P53-dependent cell-killing effect via bystander effect using carbon-ion microbeams simulating the spot scanning system with pencil beams at HIMAC, Suzuki M, Funayama T, Yokota Y, Suzuki M, Ikeda H, Sakashita T, Kobayashi Y, Murakami T, 15th International Congress of Radiation Research,   2015 05
  • Target irradiation of cells and individuals with heavy-ion microbeams of JAEA-Takasaki, Funayama T, Yokota Y, Sakashita T, Suzuki M, Ikeda H, Kobayashi Y, 15th International Congress of Radiation Research,   2015 05
  • Radiation quality-independent bystander effect and its molecular mechanism, Yokota Y, Funayama T, Ikeda H, Mutoh-Yoshihara Y, Suzuki M, Sakashita T, Kobayashi Y, The 12th International Workshop on Microbeam Probes of Cellular Radiation Response,   2015 05
  • Responses of the salt chemotaxis learning in wild type and mutant C. elegans to microbeam irradiation, Sakashita T, Suzuki M, Hattori Y, Ikeda H, Mutou Y, Yokota Y, Funayama T, Hamada N, Shirai K, Kobayashi Y, 15th International Congress of Radiation Research,   2015 05
  • The systemic effects of irradiation revealed by the local irradiation to the medaka testis using carbon-ion micro-beam system in TIARA, Oda S, Yasuda T, Watanabe-Asaka T, Ikeda H, Muto Y, Yokota Y, Sakashita T, Suzuki M, Funayama T, Kobayashi Y, Mitani H, The 12th International Workshop on Microbeam Probes of Cellular Radiation Response,   2015 05
  • Analysis of medium-mediated bystander effects induced by carbon-ion irradiation between human lung normal and cancer cells, Ikeda H, Yokota Y, Funayama T, Kanai T, Nakano T, Kobayashi Y, 15th International Congress of Radiation Research,   2015 05
  • Patterning irradiation for contact co-culture of different type cells using heavy-ion microbeam, Ikeda H, Yokota Y, Funayama T, Kanai T, Nakano T, Kobayashi Y, The 12th International Workshop on Microbeam Probes of Cellular Radiation Response,   2015 05
  • Responses of the salt chemotaxis learning in wild type and mutant C. elegans to microbeam irradiation, Sakashita T, Suzuki M, Hattori Y, Ikeda H, Mutou Y, Yokota Y, Funayama T, Hamada N, Shirai K, Kobayashi Y, The 5th International Society of Radiation Neurobiology Conference,   2015 02
  • Bystander cell-killing effect mediated by nitric oxide in normal human fibroblasts depends in part on irradiation dose but not on radiation quality, Yokota Y, Funayama T, Ikeda H, Kobayashi Y, The 60th Annual Meeting of the Radiation Research Society,   2014 09
  • Effects of carbon-ion microbeam irradiation on locomotion and pharyngeal pumping in C. elegans, Suzuki M, Hattori Y, Sakashita T, Funayama T, Yokota Y, Ikeda H, Kobayashi Y, 11th International Workshop on Microbeam Probes of Cellular Radiation Response,   2013 10
  • Analysis of bystander effects induced by heavy ions and gamma-rays, Yokota Y, Funayama T, Ikeda H, Mutoh-Yoshihara Y, Suzuki M, Sakashita T, Kobayashi Y, 11th International Workshop on Microbeam Probes of Cellular Radiation Response,   2013 10
  • Recent progress in development of heavy-ion microbeam systems of JAEA-Takasaki, Funayama T, Yokota Y, Suzuki M, Ikeda H, Sakashita T, Kobayashi Y, 11th International Workshop on Microbeam Probes of Cellular Radiation Response,   2013 10
  • Establishment of experimental systems for analyzing bystander effects induced by heavy-ion irradiation between normal and cancer cells, Ikeda H, Funayama T, Yokota Y, Kanai T, Kobayashi Y, The 11th International Workshop on Microbeam Probes of Cellular Radiation Response,   2013 10
  • Exploration of the site responsible for the radiation response of the salt chemotaxis learning in C. elegans using heavy-ion microbeam, Sakashita T, Suzuki M, Mutou Y, Hattori Y, Ikeda H, Yokota Y, Funayama T, Hamada N, Fukamoto K, Kobayashi Y, Heavy Ion in Therapy and Space Radiation Symposium 2013,   2013 05
  • Cellular response for low-fluence irradiation with HZE-ion beams, Suzuki M, Autsavapromporn N, Funayama T, Plante I, Yokota Y, Mutou Y, Suzuki M, Ikeda H, Hattori Y, Kobayashi Y, Murakami T, 3rd Asian Congress of Radiation Research (ACRR2013),   2013 05
  • Current status of heavy-ion microbeam systems of JAEA-Takasaki, Funayama T, Yokota Y, Sakashita T, Suzuki M, Ikeda H, Hattori Y, Kobayashi Y, 3rd Asian Congress of Radiation Research (ACRR2013),   2013 05
  • Radiation-quality dependent bystander effects induced by the microbeams with different radiation sources, Suzuki M, Autsavapromporn N, Usami N, Funayama T, Plante I, Yokota Y, Mutou Y, Suzuki M, Ikeda H, Hattori Y, Kobayashi K, Kobayashi Y, Murakami T, Heavy Ion in Therapy and Space Radiation Symposium 2013,   2013 05
  • Intercellular communication in the propagation of bystander effect in human fibroblasts induced by microbeams: the impact of radiation quality, Autsavapromporn N, Suzuki M, Funayama T, Usami N, Yokota Y, Mutou Y, Ikeda H, Itsuka T, Konishi T, Oikawa M, Matsufuji N, Uchihori Y, Kobayashi K, Kobayashi Y, Hei TK, Azzam EI, Murakami T, 10th International Workshop Microbeam Probes of Cellular Radiation Response,   2012 03

Misc

  • Role of High-Linear Energy Transfer Radiobiology in Space Radiation Exposure Risks., Takahashi A, Ikeda H, Yoshida Y, Int J Particle Ther., 5, 1, 151, 159,   2018 , Refereed
  • Analysis of Gamma-ray Induced Bystander Effect between Human Lung Normal and Cancer Cells, Ikeda H, Yokota Y, Funayama T, Takahashi A, Kanai T, Nakano T, Kobayashi Y, QST Takasaki Annual Report 2015, 111,   2016

Awards & Honors

  •   2017 09 , Japanese Society for Biological Sciences in Space, Young Investigator Award
  •   2012 11 , Atomic Energy Society of Japan, Kanto-Koetsu Branch (11th Young Researcher’s Presentation and Discussion Meeting), Award for Emerging Technology

Others

  •   2018 01 , Ikeda H. Support of dispatching young researchers abroad from “Living in Space”, 5 years’ fund of “Grant-in-Aid for scientific Research on Innovative Area (2015-2019) from Ministry of Education, Culture, Sports, Science and Technology Japan (MEXT), Texas, USA, January 15-31, 2018.
  •   2015 05 , Ikeda H. Support project of research for thesis in Fiscal Year 2014: Financial support for participating in “2015 Radiation Epidemiology and Dosimetry Course (May 18-22, 2015)” at the National Cancer Institute, Rockville, MD, USA, May 2015.
  •   2015 05 , Ikeda H, Financial Support for Students and Postdoctoral Fellows of the IWM2015, The 12th International Workshop on Microbeam Probes of Cellular Radiation Response, May30-June1 2015
  •   2014 11 , Ikeda H. Support project of research for thesis in Fiscal Year 2014: Supporting for PhD student, November 2014- September 2015.