 | 田中 照佳 （タナカ テルヨシ）
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水産業の活性化や地球環境保護を目的に水産物、水産加工物、水産廃棄物などから骨粗鬆症や肥満・糖尿病等の生活習慣病に有効な成分を探索しています。
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J-Global ID
研究キーワード
- 未利用資源 糖尿病 マクロファージ 破骨細胞 骨粗鬆症
現在の研究分野（キーワード）
- 水産業の活性化や地球環境保護を目的に水産物、水産加工物、水産廃棄物などから骨粗鬆症や肥満・糖尿病等の生活習慣病に有効な成分を探索しています。
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論文
- Teruyoshi Tanaka; Hanjun Tang; Kazuya Umehara; Tatsuya Moriyama; Yukio KawamuraFood Science and Technology Research 29 1 47 - 55 2023年 [査読有り]
- Teruyoshi Tanaka; Tomoki Honryo; Yoshifumi Sawada; Daniel Margulies; Vernon Scholey; Jeanne Wexler; Maria Stein; Amal Biswas; Kenji Takiifishes 7 2 2022年04月 [査読有り]
Changes in nutritional constituents and enzyme activities were clarified in yellowfin tuna (YFT, Thunnus albacares) eggs during embryonic development, from eggs immediately after fertilization to hatching. The protein levels in the eggs gradually increased with development until the completion of hatching. In contrast, the triglyceride (TG) and free amino acid (FAA) levels in the eggs gradually declined with embryonic development until hatching was complete, although the energy composition of the FAAs was lower than that of the TGs throughout embryonic development. These results indicate that endogenous TGs are preferentially expended as an energy source during embryonic development. Overall, changes in the activities of aspartate aminotransferase, alanine aminotransferase, creatine kinase, and alkaline phosphatase showed similar patterns throughout development. First, the enzyme levels diminished; then, they remained at constant, low levels just before hatching, when they rapidly increased. This rapid increase was consistent with the protein content, suggesting that organ differentiation and functionalization were promoted during this period. These results will contribute to the establishment of mass-seeding production of YFT. - Tsuyoshi Murata; Hyo Kyozuka; Shun Yasuda; Toma Fukuda; Teruyoshi Tanaka; Keiya FujimoriPLOS ONE 17 3 e0265872 2022年 [査読有り]
Ritodrine hydrochloride is used for pregnancy prolongation and intrauterine fetal resuscitation. However, its clinical significance in intraamniotic inflammation during preterm labor and intrauterine fetal distress is unclear. We investigated the effects of maternal ritodrine hydrochloride administration (MRA; 200 μg/min for 2 h, followed by 800 μg/min for 2 h after 24 h) on fetal physiological parameters. For this purpose, we used chronically instrumented pregnant sheep at 113-119 d (term = 145 d) of gestation without (Group 1, n = 5) and with (Group 2, n = 5) intraamniotic inflammation induced by lipopolysaccharide injection into the amniotic cavity. The changes in fetal heart rate (FHR) and short-term variability (STV) and long-term variability (LTV) in FHR, fetal blood pressure, and fetal arterial blood gas (FABG) values were measured before and at 1 and 2 h after initiating MRA. Before MRA, all parameters were similar between Groups 1 and 2; however, there was significantly higher STV in Group 2 than in Group 1 before MRA at 800 μg/min, significantly higher partial arterial pressure of carbon dioxide in FABG in Group 2 than in Group 1 before MRA at 200 μg/min, and significantly lower blood glucose (BG) in Group 2 than in Group 1 before MRA at 800 μg/min. One hour after MRA, the FHR, STV, and LTV were significantly higher at 800 μg/min than those at the baseline in Group 1, as determined by the Friedman test; however, no significant difference was observed in Group 2. Additionally, the FABG pH significantly decreased 1 h after MRA at 800 μg/min in Group 2, whereas FABG lactate and BG significantly increased 2 h after MRA at 800 μg/min in Groups 1 and 2. Thus, short-term MRA at 800 μg/min increased the FHR, STV, and LTV significantly; these values were further modified under intraamniotic inflammation. - Teruyoshi Tanaka; Kazuko Iwamoto; Maki Wada; Erika Yano; Toshiyuki Suzuki; Nobuhisa Kawaguchi; Norifumi Shirasaka; Tatsuya Moriyama; Yoshimi HommaMenopause (New York, N.Y.) 2021年10月 [査読有り]
OBJECTIVE: Postmenopausal women are at increased risk of metabolic diseases such as obesity and diabetes. Therefore, the chemoprevention of postmenopausal changes in health via dietary supplements is important. Syringic acid (SA) is a phenolic compound present in the fruit of the assai palm, Euterpe oleracea, and in the mycelium of the shiitake mushroom, Lentinula edodes. This compound shows no affinity for estrogen receptors and may exert disease-preventive effects. Reportedly, dietary SA ameliorates high-fat diet-induced obesity in mice; however, its effects on estrogen deficiency-induced obesity are still unclear. Therefore, in this study, we investigated whether and how dietary SA affects these factors in ovariectomized (OVX) mice. METHODS: Ten-week-old OVX mice were fed SA-containing diets (100 mg/kg body weight/d) for 12 weeks. Their body weights, food intake, and uterus weights as well as other parameters were measured and comparisons were made with mice in the control group. RESULTS: Dietary SA did not affect the body weight, food intake, or uterus weight of OVX mice over the study period; however, the SA-fed group showed lower fat mass (ie, visceral, subcutaneous, and total fat) than the OVX-control group (11.1 ± 3.3 vs. 8.3 ± 2.4, P < 0.05; 7.9 ± 1.1 vs. 5.9 ± 1.6, P < 0.05; 19.0 ± 4.2 vs. 14.1 ± 3.8, P < 0.05, respectively). Furthermore, blood analysis revealed that SA-treatment resulted in a dose-dependent decrease and increase in serum triglyceride (59.2 ± 8.3 vs. 43.9 ± 12.2 mg/dL P < 0.05) and adiponectin (7.7 ± 0.3 vs. 9.5 ± 0.6 μg/mL, P < 0.05) levels, respectively. CONCLUSIONS: These results suggest that the SA diet improves lipid metabolism without affecting the uterus in OVX mice. Therefore, dietary SA has potential applicability for the prevention of postmenopausal obesity and type 2 diabetes. - Teruyoshi Tanaka; Hiroki Onuma; Takashi Shigihara; Eiichi Kimura; Yasuhisa Fukuta; Norifumi Shirasaka; Tatsuya Moriyama; Yoshimi HommaJournal of Bioscience and Bioengineering 128 5 622 - 629 2019年11月 [査読有り]
In recent years, the number of patients with osteoporosis has increased as population grows older. Therefore, the chemoprevention of osteoporosis by better nutrition is important. White-rot fungi degrades milled wood lignin for growth and development. This degradation results in the formation of phenolic compounds such as syringic acid (SA) and vanillic acid (VA). In the artificial culture of edible mushrooms using a mushroom bed, the disposal of waste beds after mushroom cultivation is an important issue. The present study investigated the presence and amount of both SA and VA in the discarded waste beds after mushroom cultivation. The extracts from waste beds after cultivation of shiitake mushrooms, Lentinula edodes; buna shimeji, Hypsizygus marmoreus; maitake, Grifola frondosa; king trumpet mushrooms, Pleurotus eryngii; and butterscotch mushrooms, Pholiota microspora were analyzed using high performance liquid chromatography. Although the content of SA and VA was considerably different among the mushrooms, SA and VA were present in extracts obtained from all the waste beds. We also demonstrated that SA and VA exert their anti-osteoporotic effect independently of the estrogen receptor-mediated pathway using murine monocytic RAW264.7 cells, ovariectomized mice, and human breast cancer MCF-7 cells. Thus, these results suggest that the extracts are effective sources of SA and VA, which are effective in preventing osteoporosis. - Changes in the nutritional composition and organ-specific enzyme activities of laboratory-reared bluefin tuna Thunnus orientalis larvae and early juveniles.Tanaka T; Oku K; Honryo T; Takaoka O; Biswas AK; Takii KAquaculture Science 67 1 33 - 40 2019年 [査読有り]
- Changes in RNA, DNA, and protein contents in laboratory-reared yellowfin tuna, Thunnus albacaresTeruyoshi Tanaka; Nadya Morales; Tomoki Honryo; Yoshifumi Sawada; Daniel Margulies; Vernon P. Scholey; Jeanne B. Wexler; Maria S. Stein; Amal K. Biswas; Kenji TakiiAquaculture Science 67 1 33 - 40 2019年 [査読有り]
- Teruyoshi Tanaka; Matthew Kelly; Yuichiro Takei; Dai YamanouchiJournal of Vascular Surgery 68 6S 48S-59S.e1 2018年12月 [査読有り]
OBJECTIVE: Osteoclastogenic activation of macrophages (OCG) occurs in human abdominal aortic aneurysms (AAAs) and in calcium chloride-induced degenerative AAAs in mice, which have increased matrix metalloproteinase activity. As the activity of OCG in dissecting aneurysms is not clear, we tested the hypothesis that OCG contributes to angiotensin II (Ang II)-induced dissecting aneurysm (Ang II-induced AAA) in apolipoprotein E knockout mice. METHODS: AAAs were produced in apolipoprotein E knockout mice via the administration of Ang II. Additionally, receptor activator of nuclear factor kB ligand (RANKL)-neutralizing antibody (5 mg/kg) was administered to one group of mice 7 days prior to Ang II infusion. Aneurysmal sections were probed for presence of RANKL and tartrate-resistant acid phosphatase via immunohistochemistry and immunofluorescence staining. Mouse aortas were also examined for RANKL and matrix metalloproteinase 9 expression via Western blot. In vitro murine vascular smooth muscle cells (MOVAS) and murine macrophages (RAW 264.7) were analyzed for the expression of osteogenic factors via Western blot, qPCR, and flow cytometry in response to Ang II or RANKL stimulation. The signaling pathway that mediates Ang II-induced RANKL expression in MOVAS cells was also investigated via application of TG101348, a Janus kinase 2 (JAK2) inhibitor, and Western blot analysis. RESULTS: Immunohistochemical staining of Ang II-induced AAA sections revealed OCG as evidenced by increased RANKL and tartrate-resistant acid phosphatase expression compared with control mice. Immunofluorescence staining of AAA sections revealed co-localization of vascular smooth muscle cells and RANKL, revealing vascular smooth muscle cells as one potential source of RANKL. Systemic administration of RANKL-neutralizing antibody suppressed Ang II-induced AAA, with significant reduction of the maximum diameter of the abdominal aorta compared with vehicle controls (1.5 ± 0.4 mm vs 2.2 ± 0.2 mm). Ang II (1 μM) treatment induced a significant increase in RANKL messenger RNA expression levels in MOVAS cells compared with the vehicle control (1.0 ± 0.2 vs 2.8 ± 0.2). The activities of JAK2 and signal transducer and activator of transcription 5 (STAT5) were also significantly increased by Ang II treatment. Inhibition of JAK2/STAT5 suppressed Ang II-induced RANKL expression, suggesting the involvement of the JAK2/STAT5 signaling pathway. CONCLUSIONS: OCG with increased RANKL expression was present in Ang II-induced AAA, and neutralization of RANKL suppressed AAA formation. As neutralization of RANKL has been used clinically to treat osteoporosis and other osteoclast-related diseases, additional study of the effectiveness of RANKL neutralization in AAA is warranted. - Teruyoshi Tanaka; Kenji Takahashi; Kazufumi Tsubaki; Maika Hirata; Keiko Yamamoto; Amal Biswas; Tatsuya Moriyama; Yukio KawamuraFisheries and Aquatic Sciences 21 1 2018年04月 [査読有り]
In this study, we isolated and characterized the acid-soluble skin collagen of Pacific bluefin tuna (PBT, Thunnus orientalis). The PBT skin collagen was composed of two α chains (α1 and α2) and one β chain. The denaturation temperature of PBT collagen was low although it was rich in proline and hydroxyproline. The primary structure of PBT skin collagen was almost identical to that of calf and salmon skin collagen however, it differed with respect to the epitope recognition of the antibody against salmon type I collagen. These results suggest that the primary structure of skin collagen was highly conserved among animal species, although partial sequences that included the epitope structure differed among collagens. - Teruyoshi Tanaka; Nobuhisa Kawaguchi; Nobuhiro Zaima; Tatsuya Moriyama; Yasuhisa Fukuta; Norifumi ShirasakaJournal of Natural Medicines 71 4 632 - 641 2017年10月 [査読有り]
In recent years, the number of patients with osteoporosis has risen with the increase in average longevity. Therefore, the chemoprevention of osteoporosis using food materials or food components has become an increasingly important target. Syringic acid (SA) is a phenolic compound present in the fruit of the a double dagger ai palm Euterpe oleracea and the mycelium of the shiitake mushroom Lentinula edodes. This compound has no affinity for estrogen receptors and is potentially useful for disease prevention. However, little is known about the effects of a SA diet on bone metabolism, particularly bone resorption in vivo. Here, we demonstrated the effects of a SA diet on bone loss and uterine weight loss in ovariectomized (OVX) mice. Ten-week-old OVX mice were fed SA-containing diets (100 mg/kg body weight/day) for 10 weeks. After 10 weeks of dietary SA, the body weight, food intake, and uterine weight of the OVX mice were unaffected; however, femoral bone mineral density (cortical bone density, cancellous bone density, and total bone density) was higher in the SA-fed groups than in the OVX-control group. Furthermore, histomorphometric analysis revealed that the number of osteoclasts and osteoblasts was decreased and increased, respectively, in the SA-fed groups. These results suggest that a SA diet suppresses bone loss by downregulating bone resorption and upregulating bone formation without affecting the uterus in OVX mice. Although further studies are needed, SA may be a compound that can be used to prevent or retard osteoporosis. - Chitaru Kurihara; Teruyoshi Tanaka; Dai YamanouchiThe Journal of Surgical Research 214 168 - 175 2017年06月 [査読有り]
Background: Although male gender, aging, hypertension, dyslipidemia, and smoking are common risk factors for abdominal aortic aneurysm, diabetes mellitus is an independent negative risk factor. In aneurysm tissue, matrix metalloproteinases (MMPs) expressed by activated macrophages degrades extracellular matrix proteins. In our previous experimental study, we demonstrated that the aneurysmal formation and macrophage activity were suppressed by inhibiting mimicking hyperglycemia (HG) through upregulation of glucose-sensing nuclear receptor, Nr1h2. Here in this study, we focused on the role of HG-induced altered glucose uptake on macrophage activation. Methods: RAW264.7 murine macrophage cells were pretreated in cultures containing HG (HG group, 15.5mM) ornormal glucose (NG) concentrations (NG group, 5.5mM) for 7d. The culture medium was then changed in both groups to NG conditions, and the cells were stimulated with recombinant murine soluble receptor activator of NF-kappa B ligand (sRANKL). Macrophage activation was confirmed by tartrate-resistant acid phosphatase (TRAP) staining. Results: Compared with the NG group, MMP-9 expression in the HG group was significantly suppressed. Glucose uptake was increased in the NG group but not in the HG group during macrophage activation. To determine the mechanismof activation, we studied the expression and distribution of glucose transporters (Gluts) in the macrophages. Although Glut expression was unaffected by glucose pretreatment, membrane translocation of Glut-1 was significantly enhanced in macrophages in the NG group but not in the HG group during activation. Insulin receptorandinsulinreceptorsubstrate-1(IRS-1) messengerRNA, knownstimulatetomembrane translocation of Gluts, were both decreased by the HG condition but not by the NG condition. Conclusions: HG pretreatment suppressed the macrophage activation. sRANKL increased macrophage glucose uptake at NG concentrations, which was impaired by HG pretreatment through the inhibition of Glut1 membrane translocation and the insulin receptor and IRS-1 gene transcription. These data suggest that HG suppressed macrophage activation, through attenuation of glucose uptake via the suppression of the membrane translocation of Glut1 and insulin signaling. (C) 2017 Elsevier Inc. All rights reserved. - Teruyoshi Tanaka; Yuichiro Takei; Nobuhiro Zaima; Tatsuya Moriyama; Dai YamanouchiJournal of Nutritional Science and Vitaminology 63 1 28 - 34 2017年02月 [査読有り]
There have been reports that hyperglycemia suppresses osteoclast (OCL) differentiation, although the underlying mechanism is poorly understood. Here we demonstrated that high glucose suppresses OCL differentiation through activation of liver X receptor (LXR) beta, a recently reported glucose-sensing nuclear receptor. The effect of hyperglycemia on osteoclastogenesis was tested in RAW264.7 cells, a murine macrophage cell line. Cells were treated with receptor activator of NF-kappa B ligand (RANKL) under normoglycemic (5.5 mM glucose), normoglycemic with high osmotic pressure (5.5 mM glucose + 10.0 mM mannitol), and hyperglycemic (15.5 mM glucose) conditions. RANKL-induced osteoclastogenesis was significantly suppressed by high-glucose treatment. Mannitol treatment also significantly suppressed osteoclastogenesis, but the inhibitory effect was lower than for high-glucose treatment. The suppression of mRNA expression of Lxr beta by RANKL was significantly restored by high glucose, but not mannitol. Additionally, the deactivation of Lxr beta by siRNA attenuated high-glucose-induced suppression of osteoclastogenesis. Although further validation of the underlying pathway is necessary, targeting LXR beta is a potential therapeutic approach to treating osteoporosis. - Teruyoshi Tanaka; Tatsuya Moriyama; Yukio Kawamura; Dai YamanouchiJournal of Nutritional Science and Vitaminology 62 6 425 - 431 2016年12月 [査読有り]
Aneurysm is characterized by balloon-like expansion of the arterial wall and eventual rupture of the aorta. The pathogenesis of aneurysm is associated with the degradation of matrix proteins by matrix metalloproteinases (MMPs) produced by activated macrophages. Although aneurysm is associated with significant mortality and morbidity, surgical intervention is the only proven treatment strategy. Therefore, development of therapeutic agents for aneurysm is greatly anticipated. Here, we demonstrated the protective effects of the major isoflavone puerarin, which is found in kudzu roots and vines. Aneurysms were surgically induced in ten-wk-old male mice using CaPO4. Subsequently, animals were intraperitoneally injected daily with puerarin at 2.5 mg/kg body weight or with vehicle alone for 2 wk. CaPO4-induced aneurysm was significantly suppressed by puerarin administration. In subsequent macrophage activation assays using Tumor necrosis factor (TNFa) and CaPO4 crystals in vitro, puerarin decreased Mmp9 mRNA expression and secreted protein levels. Moreover, induction of IKB, ERK, and p38 phosphorylation by TNFa and CaPO4 in macrophages was suppressed by puerarin treatments. Finally, puerarin attenuated reactive oxygen species production, following induction by TNFa and CaPO4. Taken together, the present data demonstrate that puerarin suppresses macrophage activation by inhibiting IKB, ERK, and p38 activity and reactive oxygen species production in a CaPO4-induced mouse model of aneurysm. - Teruyoshi Tanaka; Yukihiro Yokota; Hanjun Tang; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraJournal of Nutritional Science and Vitaminology 62 5 341 - 349 2016年10月 [査読有り]
Postmenopausal diabetes is exacerbated by estrogen deficiency. Ovariectomized (OVX) animal models can be used to develop strategies for preventing or treating postmenopausal symptoms. We previously found that a diet containing kudzu (Pueraria lobata) vine ethanol extract (PVEE) suppressed weight gain in OVX mice. Therefore, this study further elucidated how PVEE affected OVX mice. Ten-week-old OVX or sham-operated mice were fed diets containing either no PVEE (control) or 20 mg.kg(-1).d(-1) PVEE for 8 wk, 5 mg.kg(-1).d(-1) PVEE for 24 wk, or 20 mg.kg(-1).d(-1) puerarin (daidzein-8-C-glucoside), a major isoflavone present in PVEE, for 10 wk. The effects of puerarin on glucose tolerance were also tested in OVX mice. The experimental diets were not associated with any abnormalities in any mice tested in the present study. Weight gain and serum glucose levels were increased in OVX mice and these effects were significantly attenuated in OVX mice that consumed PVEE (5 or 20 mg.kg(-1).d(-1)) or puerarin. Puerarin-treated OVX mice also showed reduced serum glucose levels following administration of 1,000 mg.kg(-1) glucose. These results suggested that puerarin contributed to PVEE-mediated improvements in glucose metabolism in OVX mice. Although further studies are needed to clarify the molecular mechanism underlying these observations, PVEE and puerarin could provide effective approaches to the amelioration of postmenopausal diabetes. - Yuichiro Takei; Teruyoshi Tanaka; K. Craig Kent; Dai YamanouchiArteriosclerosis, Thrombosis, and Vascular Biology 36 9 1962 - 1971 2016年09月 [査読有り]
Objective Arterial calcification is common and contributes to the pathogenesis of occlusive vascular disease. Similar to the dynamics of bone, it is a tightly controlled process that maintains a balance between osteogenesis and osteolysis. However, whether calcium homeostasis plays a role in the development of aneurysms has not been explored. We hypothesized that macrophages differentiate into osteoclasts in aneurysmal arteries and that protease byproducts contribute to aneurysm pathophysiology. Approach and Results We performed histological and immunohistochemical analyses and showed that macrophages positive for several osteoclast markers, including tartrate acid phosphatase, occur in great numbers in the human aneurysmal aorta, but very few occur in the human stenotic aorta and none in the nondiseased human aorta. Moreover, in situ zymography showed elevated protease activity in these cells compared with undifferentiated macrophages. Tumor necrosis factor- and calcium phosphate stimulated this osteoclastogenic differentiation process through nuclear factor-B, mitogen-activated protein kinases, and intracellular calcium signaling but not the receptor activator of the nuclear factor-B ligand. Inhibition of osteoclastogenic differentiation by bisphosphonate inhibits aneurysm development in a mouse model. Conclusions These results suggest that differentiation of macrophages into osteoclasts contributes to the pathophysiology of aneurysmal disease. - Tomoki Honryo; Teruyoshi Tanaka; Angel Guillen; Jeanne B. Wexler; Amado Cano; Daniel Margulies; Vernon P. Scholey; Maria S. Stein; Yoshifumi SawadaAquaculture Research 47 6 1832 - 1840 2016年06月 [査読有り]
Early-stage mortality due to surface water tension-related death and due to sinking to the tank bottom was investigated for yellowfin tuna, Thunnus albacares (YFT), larvae. Different aeration rates and rearing water surface conditions were examined to evaluate the effect on larval survival, swim bladder inflation and growth. The percentage survival of yolk sac larvae was significantly higher when the rearing water surface was covered with fish oil at aeration rates of 0 and 50 mL min(-1). The highest mortality occurred at the highest aeration rate of 250 mL min(-1) regardless of surface water condition. A second experiment was conducted twice under different water surface conditions: the water surface was covered by fish oil (FO), skimmed of fish oil (SS), and was not treated (NC). The percentage survival was not significantly different between treatments after 7 days of feeding. In contrast to the survival, the proportion of larvae with inflated swim bladders was significantly higher for the NC and SS groups than that of the FO group. Results of these experiments indicate that the addition of oil to the rearing water surface without its removal interferes with the initial swim bladder inflation in YFT larvae. These results also indicate that YFT larvae need to obtain (gulp) air at the water surface for initial swim bladder inflation, and success of initial swim bladder inflation may be crucial for their survival. - Teruyoshi Tanaka; Yuichiro Takei; Dai YamanouchiJournal of the American Heart Association 5 3 e003062 2016年03月 [査読有り]
Background-The aim of this study was to elucidate aspects of diabetes mellitus-induced suppression of aneurysm. We hypothesized that high glucose suppresses aneurysm by inhibiting macrophage activation via activation of Nr1h2 (also known as liver X receptor b), recently characterized as a glucose-sensing nuclear receptor. Methods and Results-Calcium phosphate (CaPO4)-induced aneurysm formation was significantly suppressed in the arterial wall in type 1 and 2 diabetic mice. A murine macrophage cell line, RAW264.7, was treated with tumor necrosis factor alpha (TNF-alpha) plus CaPO4 and showed a significant increase in matrix metalloproteinase 9 (Mmp9) mRNA and secreted protein expression compared with TNF-alpha alone. Elevated Mmp9 expression was significantly suppressed by hyperglycemic conditions (15.5 mmol/L glucose) compared with normoglycemic conditions (5.5 mmol/L glucose) or normoglycemic conditions with high osmotic pressure (5.5 mmol/L glucose + 10.0 mmol/L mannitol). Nr1h2 mRNA and protein expression were suppressed by treatment with TNF-alpha plus CaPO4 but were restored by hyperglycemic conditions. Activation of Nr1h2 by the antagonist GW3965 during stimulation with TNF-alpha plus CaPO4 mimicked hyperglycemic conditions and inhibited Mmp9 upregulation, whereas the deactivation of Nr1h2 by small interfering RNA (siRNA) under hyperglycemic conditions canceled the suppressive effect and restored Mmp9 expression induced by TNF-alpha plus CaPO4. Moreover, Nr1h2 activation with GW3965 significantly suppressed CaPO4-induced aneurysm in mice compared with vehicle-injected control mice. Conclusions-Our results show that hyperglycemia suppresses macrophage activation and aneurysmal degeneration through the activation of Nr1h2. Although further validation of the underlying pathway is necessary, targeting Nr1h2 is a potential therapeutic approach to treating aneurysm. - Teruyoshi Tanaka; Kenji Takahashi; Kohsuke Adachi; Haruki Ohta; Yukihiro Yoshimura; Yasuo Agawa; Yoshifumi Sawada; Osamu Takaoka; Amal Kumar Biswas; Kenji Takii; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraFisheries Science 80 3 603 - 612 2014年05月 [査読有り]
Type I collagen is widely distributed in most organs in teleosts. It plays a role not only in intercellular adhesion, but also in molecular signaling. In this study, Pacific bluefin tuna (PBT) procollagen alpha 1 (I) cDNA was cloned and characterized. The nine fragments of a procollagen alpha 1 (I) chain cDNA clone were prepared and spliced together to create the complete coding region. The resulting amino acid sequence was homologous with that of other teleosts. The mRNA expression profile of PBT procollagen alpha 1 (I) in various tissues and the phylogenetic analysis with other vertebrate procollagen alpha 1 (I) chains suggest that PBT procollagen alpha 1 (I) could be a precursor form of the PBT type I collagen alpha 1 chain. In addition, its level of expression in PBT larvae and early juveniles gradually increased with somatic growth. This increase was related to the standard length, wet body weight, and protein content of each individual fish. Therefore, the expression profile of procollagen alpha 1 (I) may be a useful indicator for somatic growth in fish larvae and juveniles. - Teruyoshi Tanaka; Kenji Takahashi; Naoki Iwamoto; Yasuo Agawa; Yoshifumi Sawada; Yukihiro Yoshimura; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraFisheries Science 78 4 911 - 921 2012年07月 [査読有り]
We have shown that dietary bluefin tuna skin (TUS) protects against carbon tetrachloride (CCl4)-induced hepatic damage in mice. The CCl4-induced necrotic area was decreased in mice fed a TUS-containing diet. Consistent with the decreased necrotic area, dietary TUS markedly lowered the elevated serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and the thiobarbituric acid-reactive substance (TBARS) formation induced by CCl4 injection. TUS diets also decreased phosphorylation of inhibitory kappa B-alpha and blocked the translocation of nuclear factor-kappa B to the nucleus. TUS is composed mainly (80.7 %) of type I collagen, and our results revealed that dietary tuna collagen peptides (TUCP) attenuated the increased hepatic necrotic area, serum AST and ALT activities, and liver TBARS levels induced by CCl4, similar to TUS, thus enabling us to attribute the hepatoprotective action of TUS in CCl4-intoxicated mice to tuna collagen. Therefore, TUS and TUCP may be potential food resources that are capable of alleviating hepatitis symptoms. - Seiwa Michihara; Teruyoshi Tanaka; Yuki Uzawa; Tatsuya Moriyama; Yukio KawamuraJournal of Nutritional Science and Vitaminology 58 3 202 - 209 2012年06月 [査読有り]
Puerarin, a daidzein-8-C glucoside, is the major isoflavonoid in Kudzu (Pueraria lobata), and is unique in that it contains C-C conjugated glucose at position 8 of the isoflavonoid structure. A puerarin diet at a dose of 5 mg/kg b.w./d to fed ovariectomized mice for 2 mo diminished the urinary deoxypyridinoline, a typical bone-degradation product. Since the bone absorption marker, serum tartarate-resistant acid phosphatase (TRAP) activity of puerarin-fed mice decreased but the bone formation marker, osteocalcin level, did not alter, the puerarin diet was proved to specifically depress the bone absorption, but not the overall bone metabolism. In accordance with that results, the femur structure of puerarin-fed mice was restored compared with that of puerarin-free diet mice. The atrophied uterine due to low estrogen (E2) level after ovariectomy was not restored by the puerarin diet, suggesting that puerarin exerted the anti-osteoporotic action through a non estrogen receptor (ER) mediated-pathway, in vivo. The growth of an ER-positive human breast cancer cell, MCF-70, was not enhanced by puerarin, suggesting that puerarin did not show estrogen-like action on MCF-7 cells, even at a ten thousand times higher concentration than that of E2. Furthermore, ICI182,780 (Id), an estrogen antagonist, suppressed the enhanced growth of MCF-7 cells by E2, but not that by puerarin. In an ER-binding assay, puerarin was proved not to bind to ER alpha or beta, or if all, extremely weakly, although daidzein, an aglycon of puerarin, showed a little stronger binding compared with puerarin. All these results strongly indicate that puerarin exerts its anti-osteoprotic action independently of the ER-mediated pathway. - Teruyoshi Tanaka; Hanjun Tang; Fengnian Yu; Seiwa Michihara; Yuki Uzawa; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraJournal of Agricultural and Food Chemistry 59 24 13230 - 13237 2011年12月 [査読有り]
Bone-loss-improving action of kudzu vine ethanol extracts (PVEE) was clarified. PVEE was composed roughly of 80% fiber, 10% puerarin, 3.6% daidzin, 2.5% 6 ''-O-malonyldaidzin, and the other minor isoflavones. Ten-week-old ovariectomized (OVX) mice were fed diets containing PVEE (20 mg/kg body weight/day) for 8 weeks. The bone resorption markers (urinary deoxypyridinoline and tartrate-resistant acid phosphatase activity) was elevated in OVX mice and was significantly decreased in OVX mice that consumed PVEE for 8 weeks. Consistent with the decrease in the markers, the number of matured osteoclasts in the distal femur was diminished in OVX mice fed PVEE diets. PVEE diets also suppressed the decrease in femoral bone mineral density (BMD) by OVX. PVEE showed the affinity for estrogen receptor alpha and beta 3 nearly 1/10000 weaker than 17 beta-estradiol. No hypertrophy in the uterus by the PVEE diet was observed. These results suggest that PVEE could be a promising resource for a functional food that improves osteoporosis.
MISC
- きのこ栽培過程でのシリンガ酸およびバニリン酸産生量の変化田中 照佳; 大沼 広宜; 鈴木 俊幸; 鴫原 隆; 木村 栄一; 本間 好 30 (3) 184 -189 2023年 [査読有り]
- 骨・血管組織等の代謝を制御しうる食品・栄養成分に関する研究田中照佳 日本栄養・食糧学会誌 75 (2) 71 -76 2022年 [査読有り][招待有り]
- クロマグロ皮コラーゲンの肝機能保護効果：作用物質は血中移行する食事由来ペプチドPro-Hypか？田中照佳 第6回（平成29年度）サッポロ生物科学振興財団研究助成報告 2018年
- 田中 照佳; 鈴木 佐和子; 内山 貴裕 微量栄養素研究 27 35 -38 2010年
- 魚類I型コラーゲンに関する研究河村幸雄; 田中照佳 近畿大学グローバルCOE成果報告書 2010年
産業財産権
- 特開2018-145129:脂質代謝改善用組成物及び脂質代謝改善用食品 2018年09月白坂憲章, 田中照佳, 福田泰久, 川口信久, 沼田史江
- 特開2018-145131:骨代謝改善用組成物及び骨代謝改善用食品 2018年09月白坂憲章, 田中照佳, 福田泰久, 川口信久, 沼田史江
- 特開2011-57623:骨粗鬆症予防または改善剤 2011年03月國吉智子, 河村幸雄, 野本享資, 田中照佳, 鷲田いつか
受賞
共同研究・競争的資金等の研究課題
- 高齢者・介護用食品に適した魚介類の開発－マイタケ（Grifola frondosa）による魚介類の軟化とコラーゲン分解－奨学寄附金：研究期間 : 2023年04月 -2024年03月
- 未利用トウキ茎に含有する活性成分と血糖値上昇抑制効果の解明公益財団法人ロッテ財団：奨励研究助成（B）研究期間 : 2023年04月 -2024年03月
- 日本学術振興会：科学研究費助成事業 基盤研究(C)研究期間 : 2020年04月 -2023年03月代表者 : 田中 照佳本年度は、破骨細胞を活性化した時の腹部大動脈瘤病変を観察すると共に、食品成分による腹部大動脈瘤予防効果の作用機構の解明を試みた。 女性は65歳以上になると腹部大動脈瘤に発症する可能性が高いことが知られているが、これは閉経によるエストロゲン欠乏により破骨細胞が活性化されることが原因である可能性がある。そこで、卵巣摘出マウスと卵巣摘出擬似手術マウスにおいてCaPO4誘発性の腹部大動脈瘤モデルを作成した。その結果、卵巣摘出マウスは卵巣摘出擬似手術マウスと比較し、破骨細胞が活性化され、腹部大動脈瘤の発症を促進させた。これらのデータは、「破骨細胞」は動脈瘤の発症に重要な役割を果たしていることを示唆しており、腹部大動脈瘤の発症機構の解明や予防・治療薬の開発を目指すための重要な知見になると考えられる。 C-グリコシド型イソフラボン・プエラリンは葛 （クズ、Pueraria lobata）に含有するイソフラボンである。申請者らは、プエラリンは破骨細胞の分化を抑制し、腹部大動脈瘤を予防することを報告したが、詳細な作用機構は不明である。そこで、培養細胞（RAW264.7）を用いて、プエラリンによる破骨細胞の分化抑制効果の作用機構の解明を試みた。次世代シーケンサーを用い、RNA-Seqによりプエラリンを添加した破骨細胞において発現変動する遺伝子を網羅的に解析した結果、脂質代謝関連の遺伝子発現に変動がみられた。
- クロマグロ等の養殖魚の品質ならびに健康性評価指標としてのコラーゲン代謝の解析一般財団法人東和食品研究振興会：研究期間 : 2019年04月 -2020年03月代表者 : 田中照佳
- 魚類I型コラーゲンに関する研究奨学寄付金：研究期間 : 2018年01月 -2020年03月代表者 : 田中照佳
- クロマグロ皮コラーゲンの肝機能保護効果：作用物質は血中移行する食事由来ペプチドPro-Hypか？公益財団法人サッポロ生物科学振興財団：研究期間 : 2017年04月 -2018年03月代表者 : 田中照佳
- シイタケ菌糸体に含有するシリンガ酸およびバニリン酸による骨粗鬆症予防効果と作用機構の解明公益財団法人ホクト生物科学振興財団：研究期間 : 2016年04月 -2017年03月代表者 : 田中照佳