 | 阿川 泰夫 （アガワ ヤスオ）
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Last Updated :2024/05/19
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クロマグロ養殖、クロマグロ雌雄判別、分子生物学、DNAマーカーを用いた魚類選抜育種。報道関連出演・掲載一覧
＜報道関連出演・掲載一覧＞ ●2015/10/20 テレビ朝日系列「中居正広のミになる図書館」 マグロの養殖の苦難について。
研究者情報
学位
ホームページURL
J-Global ID
研究キーワード
- 遺伝学 生化学 分子生物学 脂質代謝 水産魚類遺伝マーカー調査
現在の研究分野（キーワード）
- クロマグロ養殖、クロマグロ雌雄判別、分子生物学、DNAマーカーを用いた魚類選抜育種。
研究分野
- ライフサイエンス / 水圏生命科学
- ライフサイエンス / 応用生物化学
- ライフサイエンス / 発生生物学
- ライフサイエンス / 分子生物学
- ライフサイエンス / システムゲノム科学
- ライフサイエンス / ゲノム生物学
経歴
学歴
所属学協会
研究活動情報
論文
- Spawning ecology of captive yellowfin tuna broodstock inferred by the use of mitochondrial DNA sequencing analysisSusana Cusatti; Daniel Margulies; Vernon Scholey; Yoshifumi Sawada; Yasuo Agawa70 4 331 - 342 2022年12月 [査読有り]
- Tomoki Honryo; Shigekazu Katayama; Yasuo Agawa; Yoshifumi SawadaAquaculture 560 738585 - 738585 2022年11月
- Yasuo Agawa; Takahito Saiki; Taimu Miyamoto; Yuuma Ikeue; Tomoki Honryo; Yoshifumi SawadaFisheries Science 88 2 319 - 327 2022年03月 [査読有り]
- Hyojin Ahn; Yasuo Agawa; Keitaro KatoComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 262 111075 - 111075 2021年12月 [査読有り]
- Tomoki Honryo; Yuki Sakurai; Saki Yamao; Tokihiko Okada; Yasuo Agawa; Yoshifumi SawadaMarine Pollution Bulletin 170 112679 - 112679 2021年09月
- Yoshifumi Sawada; Yuga Mizushima; Takashi Sera; Tomoki Honryo; Michio Kurata; Yasuo AgawaAquaculture Research 2021年08月
- Kazuo Ogawa; Hirotaka Mizuochi; Tomofumi Yamaguchi; Sho Shirakashi; Nanami Asai; Yasuo AgawaFISH PATHOLOGY 55 4 117 - 124 2021年01月 [査読有り]
The grow-out stage of leopard coral grouper Plectropomus leopardus reared in land based tanks in Okinawa Prefecture, Japan, were often heavily infected with capsalid monogeneans, up to a few millimeters in size, on the fins and skin. Morphological investigation revealed that the parasite belongs to Benedenia (Monogenea: Capsalidae). Benedenia akajin n. sp. is proposed here and described morphologically, based on the unique structures in the vaginal duct with a thick-walled distal one-third, the penis with a teat-shaped tip and the testes smaller than the germarium. A positive relationship between the lengths of three haptoral sclerites (anterior sclerites and anterior and posterior hamuli) and body length indicates that these taxonomically important structures keep growing as the parasite ages. Molecular analyses showed that the present species is most closely related to B. epinepheli and B. sargocentron, with the highest 28S rDNA identity of 98.1%, but no identical sequences were found in the database. Both morphological and molecular evidences suggest Benedenia akajin n. sp. as an undescribed species, and this new skin fluke deserves further investigations for the stable aquaculture of P. leopardus. - 本領智記; 塚田ほなみ; 岡田貴彦; 阿川泰夫; 倉田道雄; 澤田好史水産増殖 68 4 317 - 326 2020年12月 [査読有り]
- Yoshifumi Sawada; Tsukasa Sasaki; Keisuke Nishio; Michio Kurata; Tomoki Honryo; Yasuo AgawaAquaculture Research 51 6 2261 - 2274 2020年06月
- Tomoki Honryo; Michio Kurata; Shigekazu Katayama; Osamu Takaoka; Yasuo Agawa; Yoshifumi SawadaAquaculture Research 51 1 432 - 435 2020年01月
- Tomoki Honryo; Tokihiko Okada; Michio Kurata; Yasunori Ishibashi; Yasuo Agawa; Yoshifumi SawadaAquaculture 506 355 - 358 2019年05月 [査読有り]
- Tsubasa Uchino; Erina Hosoda; Yoji Nakamura; Motoshige Yasuike; Miyuki Mekuchi; Masashi Sekino; Atushi Fujiwara; Takuma Sugaya; Yosuke Tanaka; Kazunori Kumon; Yasuo Agawa; Yoshifumi Sawada; Motohiko Sano; Takashi SakamotoAquaculture Research 49 3 1293 - 1301 2018年03月 [査読有り]
Pacific bluefin tuna (Thunnus orientalis) has high market value, but its wild populations have decreased in recent years. The broodstock of Pacific bluefin tuna that were hatched artificially and reared under aquaculture conditions is beginning to be used for production. The creation of broodstock with commercially valuable traits, such as rapid growth, is therefore of great interest. Genetic linkage map-based identification of markers associated with quantitative trait loci (QTLs) facilitates marker-assisted selection (MAS) breeding and allows efficient genetic improvement of broodstock. Single nucleotide polymorphism (SNP)-based genetic linkage map construction using the genotyping-by-sequencing method can expand the number of mapped markers and help identify growth-related QTLs. In this study, we constructed sex-specific maps for 24 linkage groups consisting of 677 SNP and 651 microsatellite markers. The total lengths of 93 progenies in the mapping population followed normal distribution, with an average length of 9.4 mm. We performed composite interval mapping in the mapping population. QTL analysis revealed one significant QTL in LG10 on the female linkage map. The genetic linkage map—the second such map generated for Pacific bluefin tuna—and the growth-related QTLs detected in this study will be useful for tuna aquaculture MAS programs. - Maria S. Stein; Daniel Margulies; Jeanne B. Wexler; Vernon P. Scholey; Katagiri Ryo; Tomoki Honryo; Tsukasa Sasaki; Angel Guillen; Yasuo Agawa; Yoshifumi SawadaJournal of the World Aquaculture Society 49 1 240 - 255 2018年02月 [査読有り]
Three experiments were conducted to investigate the growth, survival, and standardized cohort biomass of Pacific bluefin tuna, Thunnus orientalis, larvae fed nutritionally enhanced prey during the first week of feeding using two commonly used, commercially available enrichment media, AlgaMac Enrich and Marine Glos. T. orientalis larvae exhibited exponential growth in standard length and dry weight. The daily specific growth rates in length and weight are the first reported for T. orientalis larvae and the averages ranged from 3.8 to 4.1% and 27.5%, respectively, for larvae in the AlgaMac treatment and from 4.1 to 6.1% and 31.5%, respectively, in the Marine Glos treatment. Average daily growth rates in length ranged from 0.16 to 0.23 mm/d for larvae in the AlgaMac treatment and from 0.17 to 0.27 mm/d for those in the Marine Glos treatment. Daily growth rates in length were similar to those reported for other tuna larvae reared in the laboratory but slower than most published estimates for larval tunas in situ at similar water temperatures. Mean prey number per gut was positively associated with mean prey level in the tank. Both enrichment media appear to be good sources of nutritional improvement of planktonic prey for T. orientalis larvae. - 澤田 好史Aquaculture 484 175 - 183 2018年02月 [査読有り]
It is important to understand the physiological stress responses in juvenile Pacific bluefin tuna (PBF), Thunnus orientalis, when transporting them from land-based tanks to sea cages, to develop countermeasures against mass mortality and enhance survival. We investigated the estimated time required to recover from transportation stress in PBF juveniles (32 and 35 days post hatch (DPH)) transported over two different distances (Exp 1; long distance (15.2 km), Exp 2; short distance (2.3 km)). Additionally, we examined the effects of simulated transport in fish that were transported from tank-to-tank, as well as starvation tolerance to determine the causes of stress responses during transportation. Whole-body cortisol levels and HIF-1 alpha expression levels in gills increased from pre-transport levels shortly after juveniles were released into the sea cage in Exp 1, but recovered within 24 h. Simultaneously, whole-body glucose levels fell over the 48 h after transport. Gene expression of the Hsp70 family in the liver was upregulated 48 h after fish were released into the sea cages in Exp 1, and was significantly upregulated after 48 h in Exp 2. These results indicated that 72 h were required for PBF juveniles to recover from transportation to sea cages. The starvation experiment suggested that PBF juveniles were significantly affected after 2 days of fasting and that the environmental conditions in the sea cage were the main factors resulting in a decrease in whole-body glucose levels and upregulation of Hsp70-family gene expression. Furthermore, changes of proximate compositions indicated that PBF juveniles consumed crude lipids and proteins, which were significantly affected by fasting for 1-2 days. We suggested that the first 3 days after stocking are a critical period for recovery from transportation stress and it is necessary to develop appropriate management techniques to alleviate the high mortality caused by these stress responses. - Yasuo Agawa; Toshio Kaga; Shigekazu Katayama; Tokihiko Okada; Yoshifumi SawadaAQUACULTURE RESEARCH 48 7 3320 - 3328 2017年07月 [査読有り]
Pacific bluefin tuna (PBF), Thunnus orientalis, is commercially one of the most important species of tuna. In this study, amplified fragment length polymorphism (AFLP) screening was conducted to find the growth-related polymorphic DNA in cultured PBF. Fish hatched in 2007 were harvested at an age of 818-1994days. They were categorized into superior, average and inferior growth groups, depending on their growth score at the time of harvest. On AFLP screening of 24 fish, with eight fish from each group, 215 polymorphic DNA fragments were observed. A second amplification, with EcoRI+ACC and MseI+CCC primers, generated a polymorphic fragment of 630bp at a rate of 80.0% (n=15) in the superior, 56.3% (n=16) in the average and 20.0% (n=15) in the inferior growth groups. Polymerase chain reaction (PCR) primers, which could amplify both AFLP-positive and AFLP-negative loci, were developed using the consensus sequence outside the AFLP target fragment. Eleven haplotypes were obtained by sequence analysis of the PCR product at the AFLP target loci. Among those, haplotype 1 was statistically significant in the superior and average growth groups and could be used as a molecular marker for distinguishing the individuals with superior and average growth from those with inferior growth. - Yasuo Agawa; Kazuki Tani; Syuhei Yamamoto; Chihaya Hirano; Sho ShirakashiFISHERIES SCIENCE 82 5 827 - 833 2016年09月 [査読有り]
Skin fluke Neobenedenia girellae infection is a chronic problem in marine finfish aquaculture. Current control measures rely on bath treatments, which are labor intensive and cause significant stress to fish. Although there is an urgent need to develop a strategy to prevent this infection, little is known about the distribution of the larval stage of the skin fluke around culture cages. We aimed to develop a Neobenedenia-specific real-time quantitative polymerase chain reaction (qPCR) assay to detect and quantify N. girellae larvae in environmental water. New PCR primers targeting mitochondrial DNA were designed and showed specificity for N. girellae among five capsalid monogeneans known to occur in Japanese waters. A single N. girellae oncomiracidium is estimated to carry 2.2 million copies of mitochondrial DNA, and the qPCR assay reliably detects DNA equivalent to half of that. We used this qPCR to quantify N. girellae larvae from a fish culture site. Analyses of water samples collected inside shaded culture cages of Seriola dumerili revealed lower larval density than that in the unshaded cage. The results indicate the usefulness of this newly developed qPCR assay for monitoring skin fluke larval density and distribution in fish farms. - Effect of dietary taurine enhancement on growth and development in red sea bream Pagrus major larvaeYang-Su Kim; Tsukasa Sasaki; Masato Awa; Maho Inomata; Tomoki Honryo; Yasuo Agawa; Masashi Ando; Yoshifumi SawadaAQUACULTURE RESEARCH 47 4 1168 - 1179 2016年04月 [査読有り]
This study investigated the effect of feedings taurine-enriched rotifers on the growth and development of larval red sea bream (RSB). Rotifers incubated in taurine-enriched water at a taurine concentration of 800mgL(-1) (T-800) and 0mgL(-1) (T-0) were fed to larvae from 3 to 20days after hatching (DAH). Notochord length, body weight and specific growth rate of T-800 group were significantly greater than those of T-0 at 14, 17, 9-11 and 18-20 DAH. Taurine content of larvae in the T-800 group increased rapidly from 11 DAH and thereafter remained significantly higher than T-0. Flexion larvae firstly appeared in both groups at 8 DAH, however, at 20 DAH post-flexion larvae were significantly more abundant in T-800 than T-0. While nucleic acid and protein contents (gmg(-1) wet fish) showed remarkable changes, ontogenetic growth in RSB larvae stage was observed to switch from hyperplastic growth to hypertrophic growth with the start of the flexion stage. Although a similar change in nucleic acid contents was observed between the two groups, the protein content (gfish(-1)) and protein/DNA ratio of T-800 remained higher than that of T-0 during the hypertrophic growth period. These results suggest that dietary taurine accelerates the growth and development in RSB larvae especially during hypertrophic growth (flexion stage) after the early hyperplastic growth. - Toru Kobayashi; Tomoki Honryo; Yasuo Agawa; Yoshifumi Sawada; Ileana Tapia; Karla A. Macias; Amado Cano; Vernon P. Scholey; Daniel Margulies; Naoki YagishitaREPRODUCTIVE BIOLOGY 15 2 106 - 112 2015年06月 [査読有り]
To develop techniques for seedling production of yellowfin tuna, the behavior of primordial germ cells (PGCs) and gonadogenesis were examined at 1-30 days post hatching (dph) using morphometric analysis, histological examination, and in situ hybridization. Immediately after hatching, PGCs were located on the dorsal side of the posterior end of the rectum under the peritoneum of the larvae, and at 3 dph they came into contact with stromal cells. PGCs and stromal cells gradually moved forward from the anus prior to 5 dph. At 7-10 dph, germ cells were surrounded by stromal cells and the gonadal primordia were formed. In individuals collected at 12 dph, PGCs were detected by in situ hybridization using a vasa mRNA probe that is a germ-cell-specific detection marker. The proliferation of germ cells in the gonadal primordia began at 7-10 dph. We observed double the number of germ cells at 30 dph (22 +/- 3.2 cells), compared to that at 1 dph (11 +/- 2.1 cells). Therefore, based on our data and previous reports, the initial germ cell proliferation of yellowfin tuna is relatively slower than that of other fish species. (C) 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All rights reserved. - Yoshifumi Sawada; Yasuo AgawaAdvances in Tuna Aquaculture: From Hatchery to Market 323 - 332 2015年01月 [査読有り]
Efforts to develop techniques for breed improvement in aquaculture have been conducted on numerous species, including salmonids, tilapias, and carps. Although attempts at breed improvement for tuna only recently began, rearing technologies used to close the Pacific bluefin tuna (PBFT) life cycle could potentially expedite the development of PBF breed improvement. The PBFT breeding program at Kinki University currently targets the following desirable traits: improved reproduction, increased survival rate during early larval development, faster growth rates, enhanced disease resistance, and improved meat quality. This technology emphasizes the use of marker-assisted breeding programs based on genomic and mitochondrial DNA analysis conducted on carefully selected fish held in bio-secure oceanic net pens. This approach prevents the potential "genetic pollution" of natural PBFT populations from encounters with escaped fish. Together with PBFT breed improvement, the simultaneous development of stress-free (reduced) handling technologies for activities such as juvenile tagging is also essential as tuna species have a low tolerance for handling stress. Finally, a comprehensive strategy for intellectual property protection including copyrights, patents, and trademarks should be implemented in any novel breeding program such that the hard-earned products and technologies developed can be protected from unfair competition. - 松本 太朗; 阿川 泰夫; 岡田 貴彦; 澤田 好史; 石橋 泰典Aquaculture Science 63 2 179 - 189 2015年 [査読有り]
- Yasuo Agawa; Mayui Iwaki; Takafumi Komiya; Tomoki Honryo; Kouhei Tamura; Tsukasa Okada; Naoki Yagishita; Toru Kobayashi; Yoshifumi SawadaFISHERIES SCIENCE 81 1 113 - 121 2015年01月 [査読有り]
A male-specific marker of a DNA sequence for the Pacific bluefin tuna Thunnus orientalis, Male delta 6 (Md6), was identified by comparative study of the sequences obtained by F3-cultured male and female AFLP-selective DNA amplification products, followed by high-throughput DNA sequencing. Md6 was characteristic in continuous 6-bp nucleotide deletions compared to redundant sequences that could be seen in both sexes. The PCR genotyping primers were developed based on the Md6 sequence, which showed that 29 of 32 F3 males (90.6 %) and 9 of 13 (69.2 %) F2 parental males were genotyping positive, whereas in females, the genotyping test was negative in parental F2 (n = 12), and 1 of 32 (3.1 %) F3 female was Md6-positive. Parentage allocation tests suggested that Md6-positive F2 males were more attendant in spawning than Md6-negative males, suggesting that Md6 was passed from male parents to male progeny under aquaculture conditions. - Shohei Nomura; Toru Kobayashi; Yasuo Agawa; Daniel Margulies; Vernon Scholey; Yoshifumi Sawada; Naoki YagishitaFISHERIES SCIENCE 80 6 1193 - 1204 2014年11月 [査読有り]
The genetic population structure of the Pacific bluefin tuna (PBF) Thunnus orientalis and the yellowfin tuna (YFT) T. albacares in the North Pacific Ocean was investigated. The polymorphism of microsatellite (SSR) loci and sequences of mitochondrial DNA control region (mtCR) were analyzed for 71 samples of PBF from Japan and Mexico and 45 samples of YFT from Japan and Panama. In the SSR analyses, both single-locus (-0.010 to 0.008 in PBF and -0.023 to 0.020 in YFT) and global multilocus (0.003 in PBF and -0.002 in YFT) F-ST values among the geographic populations were low and not significant in these species. In the mtCR analyses, neither the neighbor-joining tree nor the minimum spanning network showed genetic differentiation among the geographic populations in each species. The pairwise F-ST values among the geographic populations of them (-0.005 in PBF and -0.020 to -0.014 in YFT) were low and not significant. Our SSR and mtCR data suggested that genetic differentiations were not evident among the eastern and western populations in the North Pacific Ocean either in PBF or in YFT. Mismatch distributions, demographic parameters, and neutrality tests suggested that sudden population expansion of PBF and YFT in the North Pacific Ocean occurred 628,000-731,000 and 450,000-525,000 years ago, respectively. - Bimol C. Roy; Yasuo Agawa; Heather L. Bruce; Masashi Ando; Tokihiko Okada; Yoshifumi Sawada; Tomohiro Itoh; Yasuyuki TsukamasaFISHERIES SCIENCE 80 5 1009 - 1020 2014年09月 [査読有り]
With the development of successful aquaculture techniques, the Pacific bluefin tuna (PBT) has become the most important fish species in Japan. Early muscle growth and cellularity of muscle fibers have a profound effect on ultimate body size and meat quality of fish. Larvae and juveniles of full-cycle cultured PBT were sampled at 3, 15, 29, 41, 70, 128 and 218 days post hatch (dph). Transverse body sections until 41 dph and muscle sections from 41 dph thereafter from dorso-cranial or caudal areas were stained for muscle fiber morphological and morphometric analysis and muscle sections from the dorso-cranial region were used for ultra-structural observation. Red muscle fibers appeared by 15 dph at the horizontal septum both in epaxial and hypaxial regions. Other than a single layer of superficial red fibers and those at the horizontal septum, existence of red muscle fibers was apparent in the PBT myotome. The muscle fiber diameters varied in their size even in adjacent myotomes of PBT. Overall, growth throughout the various stages was a result of both hyperplasia and hypertrophy of muscle fibers. Muscle fiber diameters were greater in the dorso-cranial than in dorso-caudal region at 41, 70 and 128 dph. These results provide additional characterization of PBT growth, which may assist with managing fish production efficiency, harvest age and eating quality. - 岡田 貴彦; 本領 智記; 澤田 好史; 阿川 泰夫; 宮下 盛; 石橋 泰典Aquacultural Engineering 59 23 - 25 2014年03月 [査読有り]
Although the survival rate of juvenile Pacific bluefin tuna Thunnus orientalis (PBT) during 30 days after stocking in sea net cages has been as low as approximately 50%, the reason for this high mortality is not clear. The dead fish were collected and counted during 30 days after stocking in a sea net cage, and the growth performance, stomach contents, and bone injury were investigated. Nearly half (47.9%) had died within the first 4 days. The total length and body weight of the dead fish were significantly smaller than those of the live fish, and the weight of the dead fish did not increase during the first 7 days. Approximately one-third of the dead fish (21.9-42.9% per day) had ingested inorganic matter such as wood or Styrofoam waste during the first 7 days, and 25.0-45.0% of the dead fish sampled per day showed poor growth. The daily ingestion rate of inorganic matter decreased dramatically from the eighth day, when the daily mortality rate decreased. The percentage of dead fish with bone injuries due to collision or contact was low (less than 15%). These results suggest that one of the causes of high mortality during several days after stocking in a sea cage was accidental ingestion of inorganic matter by juvenile PBT. Improvement in feeding method can increase the survival rate of juveniles in sea net cages. (C) 2014 Elsevier B.V. All rights reserved. - Naoki Yagishita; Yoshifumi Sawada; Yasuo Agawa; Toru KobayashiCONSERVATION GENETICS RESOURCES 6 1 189 - 191 2014年03月 [査読有り]
The pacific bluefin tuna (PBT) Thunnus orientalis is one of the most important species for fisheries in the world. This species has been highly exploited in fisheries, resulting in dwindling of its natural resources. The artificial hatching of a third generation of fully cultured PBT was a success in 2007, suggesting the possibility of mass production of PBT for food and for seed release. To enhance stock structure studies and investigate genetic feature of seedlings, we isolated 25 polymorphic microsatellite loci (2-18 alleles/locus; expected heterozygosity, 0.042-0.941) from PBT. - 堤 吉輝; 松本 太朗; 本領 智記; 阿川 泰夫; 澤田 好史; 石橋 泰典Environmental Biology of Fishes 97 1 53 - 58 2014年01月 [査読有り]
The spectral sensitivity of the fish and the suitable light wavelength range for survival and growth performance of juvenile Pacific bluefin tuna (PBT) were investigated. The spectral sensitivity peak of PBT under photopic condition was observed between 449 and 503 nm, which corresponded to their natural habitat. The fish were reared in tanks irradiated continuously with 4 kinds of light emitting diodes (LEDs). The maximum wavelength of LEDs used for the rearing experiment were 460 nm (blue), 520 nm (green), 630 nm (red), and 450-680 nm (white). There was no notable difference in survival rate among fish in the four LED groups. However, the growth of juvenile PBT was lesser under red light compared to the green and white light wavelengths. These results suggest that PBT juveniles have low sensitivity to red light because the fish are rarely exposed to the red light wavelengths under natural ocean conditions. Thus, low sensitivity to red light negatively influenced the feeding behavior and growth of PBT juveniles. - Teruyoshi Tanaka; Kenji Takahashi; Kohsuke Adachi; Haruki Ohta; Yukihiro Yoshimura; Yasuo Agawa; Yoshifumi Sawada; Osamu Takaoka; Amal Kumar Biswas; Kenji Takii; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraFisheries Science 80 3 603 - 612 2014年 [査読有り]
Type I collagen is widely distributed in most organs in teleosts. It plays a role not only in intercellular adhesion, but also in molecular signaling. In this study, Pacific bluefin tuna (PBT) procollagen α1 (I) cDNA was cloned and characterized. The nine fragments of a procollagen α1 (I) chain cDNA clone were prepared and spliced together to create the complete coding region. The resulting amino acid sequence was homologous with that of other teleosts. The mRNA expression profile of PBT procollagen α1 (I) in various tissues and the phylogenetic analysis with other vertebrate procollagen α1 (I) chains suggest that PBT procollagen α1 (I) could be a precursor form of the PBT type I collagen α1 chain. In addition, its level of expression in PBT larvae and early juveniles gradually increased with somatic growth. This increase was related to the standard length, wet body weight, and protein content of each individual fish. Therefore, the expression profile of procollagen α1 (I) may be a useful indicator for somatic growth in fish larvae and juveniles. © 2014 The Japanese Society of Fisheries Science. - Teruyoshi Tanaka; Kenji Takahashi; Naoki Iwamoto; Yasuo Agawa; Yoshifumi Sawada; Yukihiro Yoshimura; Nobuhiro Zaima; Tatsuya Moriyama; Yukio KawamuraFISHERIES SCIENCE 78 4 911 - 921 2012年07月We have shown that dietary bluefin tuna skin (TUS) protects against carbon tetrachloride (CCl4)-induced hepatic damage in mice. The CCl4-induced necrotic area was decreased in mice fed a TUS-containing diet. Consistent with the decreased necrotic area, dietary TUS markedly lowered the elevated serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and the thiobarbituric acid-reactive substance (TBARS) formation induced by CCl4 injection. TUS diets also decreased phosphorylation of inhibitory kappa B-alpha and blocked the translocation of nuclear factor-kappa B to the nucleus. TUS is composed mainly (80.7 %) of type I collagen, and our results revealed that dietary tuna collagen peptides (TUCP) attenuated the increased hepatic necrotic area, serum AST and ALT activities, and liver TBARS levels induced by CCl4, similar to TUS, thus enabling us to attribute the hepatoprotective action of TUS in CCl4-intoxicated mice to tuna collagen. Therefore, TUS and TUCP may be potential food resources that are capable of alleviating hepatitis symptoms.
- Yasuo Agawa; Tomoki Honryo; Ayako Ishii; Toru Kobayashi; Hiromi Oku; Yoshifumi SawadaAQUACULTURE RESEARCH 43 8 1145 - 1158 2012年07月 [査読有り]
Pacific bluefin tuna (PBT) peroxisome proliferator-activated receptor ? (PPAR?) gene was characterized to know the expression of its transcript contribution to the development of PBT, because PPAR? is the key molecule for adipose cell differentiation. Resulting cDNA and deduced amino acid sequence had high similarities to other teleosts that consisted of A to F domains. Phylogenetic tree analysis indicated a close relationship PBT PPAR? to cobia and flatfishes ones among the teleosts with the similarity of characteristic insertion sequences at amino terminal region of E/F domains. PPAR? transcripts quantification profile in the tissues of a 13-month-old PBT indicated the correlation between its quantity with the muscle types of different lipid content. The transcripts were also detected in the head kidney, digestive organs and red muscle with higher level inferred the PPAR? contribution to the multiple physiological processes. PPAR? transcripts were quantified for PBT juveniles fed artificial diets to investigate the effect of phospholipid supplementation on the growth, survival and PPAR? expression, to verify the efficacy of phospholipid source. No obvious difference in growth performance, survival and gene expression of PBT juveniles was identified, this may be due to DHA/EPA ratio analogy between diets and indicating that phospholipid sources in the juvenile diet might be adaptable. - Yoshifumi Sawada; Kuzuhiro Higuchi; Tomoki Honryo; Yasuo Agawa; Hirokazu Shiba; Tatsuo Yokoyama; Hidemi KumaiAQUACULTURE 350 33 - 36 2012年06月 [査読有り]
Morphogenesis is most active during fish embryonic development with increased susceptibility to environmental stressors. In order to elucidate the causalities of malformations in fishes that produce buoyant eggs, this study estimated the time change of dissolved oxygen (DO) and carbon dioxide (DCD) concentrations in the interstitial water of red sea bream, Pagrus major, egg aggregation. Since direct measurement of DO and DCD concentrations is not possible by current methodologies, we measured those in one liter water containing approximately 500,000 eggs for their estimation. Calculated oxygen consumption and carbon dioxide excretion rates from observed data were 1.32x10(-6) and 2.16x10(-7) mg/ind/min, respectively. Volumetric method with these numeric values estimated that DO decreases below 10% saturation within 21 s and DCD increases to more than 135 mg/L within 60 min in the interstitial water of egg aggregation. These results suggest that hypoxia and hypercapnia may become teratogenic within a short time in the aquaculture practices of egg collection, treatment, and transportation where eggs aggregate at the water surface in containers or rearing tanks, or in the wild when eggs are exposed to red tide in the open sea. (C) 2012 Elsevier B.V. All rights reserved. - 阿川 泰夫日本水産学会誌 77 4 639 - 646 2011年07月 [査読有り]
AFLP, amplified fragment length polymorphism screening, was conducted to identify sex characteristic DNA markers of cultured Pacific bluefin tuna (PBT), Thunnus orientalis. The AFLP screening of 4 females and 4 males with 64 primer combinations produced a total of 1038 scorable bands, of which 183 were polymorphic between individuals. Male characteristic fragments were confirmed with EcoRI + AGG and MseI + CAT primers by gel image analysis, which corresponded to 437 bp in 29 of 32 males and 3 of 32 females. Fragment detail was analyzed by electropherogram analysis and DNA quantification. The 437 bp fragment was detected in 30 individuals of average DNA concentration 0.231 ng/mu L; while in females, 13 individuals were detected with average 0.080 ng/mu L (P<0.01). - Yasuo Agawa; Moustafa Sarhan; Yuji Kageyama; Kazutaka Akagi; Masayoshi Takai; Kazuya Hashiyama; Tadashi Wada; Hiroshi Handa; Akihiro Iwamatsu; Susumu Hirose; Hitoshi UedaMOLECULAR AND CELLULAR BIOLOGY 27 24 8739 - 8747 2007年12月 [査読有り]
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway. - Y Kono; Y Agawa; Y Watanabe; E Ohama; E Nanba; K NakashimaINTERNAL MEDICINE 38 5 407 - 411 1999年05月This study was performed to confirm 1) the difference in the trinucleotide CAG repeat number among tissues, 2) somatic mosaicism in each tissue, 3) the correlation of the repeat number with pathological severity in Huntington's disease, The CAG repeat number was determined by analysis of the polymerase chain reaction (PCR) product in various tissues, including central nervous system (CNS) tissues and non-CNS tissues, We also determined the pathological severity grade in each brain section and compared this with the results of CAG repeat analyses. The patient was a Japanese male with Huntington's disease who died at 62 years of age. Genomic DNA was extracted from 10 parts of the central nervous system and 6 parts pf other tissues from the patient. Each part of the formalin-fixed brain was subjected to gross and microscopic pathological assessment. The main peaks of CAG repeat in all tissues were 22 and 44, In analysis of somatic mosaicism, high degrees of mosaicism were obtained in the caudate nucleus, putamen and cerebral cortex, in which more severe degeneration was observed by pathological examination. These results, although this is a single case study, indicated that pathological severity did not correlate with the CAG repeat number, but it did relate to the degree of somatic mosaicism, Somatic mosaicism might reflect region-specific neuronal degeneration in Huntington's disease.
書籍
- Advances in Tuna Aquaculture阿川 泰夫 (担当:共著範囲:Chapter13 Genetics in Tuna Aquaculture)Academic Press 2015年
MISC
- シマアジ親魚推定法(マイクロサテライト、dd-RAD-seq)の比較と優良親魚推定について阿川泰夫; 谷山直輝; 前田知隆; 源六佳志; 澤田好史 令和6年度春季大会要旨集 2024年03月
- 優良魚DNA解析手法の技術進展と応用について阿川泰夫 令和6年度第1回水産増殖談話会シンポジウム 2024年03月 [招待有り]
- クロマグロ稚魚の成長モニタリング試験-体表粘液標本のdd-RAD-seq法を用いて-阿川 泰夫; 河原 正人; 木南 竜平; 澤田 好史; 白澤健太; 磯部 祥子 日本分子生物学会要旨集 2023年12月
- クロマグロ幼魚体表粘液標本の dd-RAD-seq 法を用いた個体識別試験阿川泰夫; 河原正人; 木南竜平; 青木隆一郎; 澤田好史; 白澤健太; 磯部祥子 令和5年度秋期日本水産学会要旨集 2023年09月
- 養殖海域におけるハダムシのモニタリング阿川泰夫, 山本剛, 澤田好史, 白樫正 令和5年度日本水産学会春季大会要旨集 2023年03月
- クロマグロ稚魚の成長モニタリング試験-体表粘液標本のdd-RAD-seq法を用いて-阿川 泰夫, 河原 正人, 木南 竜平, 澤田 好史, 白澤健太, 磯部 祥子 ゲノム微生物学会要旨集 2023年03月
- シマアジ養殖集団のSNPを用いた個体識別と遺伝的クラスター解析阿川泰夫; 澤田好史 第1回 超分野植物科学研究会 (TDPS)要旨集 2021年06月
- 阿川泰夫; 日原麻衣; 澤田好史; 白樫正; 水落裕貴; 小川和夫 日本水産学会大会講演要旨集(CD-ROM) 2021 2021年
- 阿川泰夫; 日原麻衣; 澤田好史; 白樫正 日本水産学会大会講演要旨集 2020 2020年
- 阿川泰夫; 前田知隆; 八木洋樹; 谷口亮人; 本領智記; 倉田道雄; 澤田好史 日本水産学会大会講演要旨集 2019 2019年
- 澤田好史; 坂本亘; 津田裕一; 藤岡紘; 福田漠生; 本領智紀; 阿川泰夫; 岡田貴彦; 光永靖; 熊井英水 日本水産学会大会講演要旨集 2018 168 2018年03月
- 阿川泰夫; 谷和樹; 山本修平; 平野千早; 澤田好史; 白樫正 日本水産学会大会講演要旨集 2017 2017年
- 澤田 好史; 阿川 泰夫; 福岡 祐大; 本領 智紀; 倉田 道雄; 岡田 貴彦 近畿大学水産研究所報告 (16) 1 -10 2016年03月
- 澤田 好史; 加賀 俊生; 阿川 泰夫; 本領 智記; 金 良洙; 中谷 正宏; 岡田 貴彦; Cano A.; Margulies D.; Scholey V. 水産増殖 61 (3) 315 -319 2013年09月
- クロマグロ若齢期における成長とIGF-1遺伝子発現.佐伯 康; 岡田貴彦; 本領智記; 阿川泰夫; 澤田好史; 柳下直己; 小林 徹 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2012(平成20-24)年度最終成果報告書 388 -390 2013年
- 養殖クロマグロThunnus orientalisの遺伝的多様性と家系判別．小林 徹; 阿川泰夫; 澤田好史 グローバルCOEプログラム クロマグロ等の養殖科学の国際教育研究拠点2008-2009(平成20-21)年度中間成果報告書 145 -146 2010年
産業財産権
共同研究・競争的資金等の研究課題
- DNAマーカーを用いた親魚管理文部科学省：私立大学戦略的研究基盤形成支援事業研究期間 : 2014年04月 -2017年03月代表者 : 升間主計
- クロマグロ産卵親群編成の為の、精度良い雌雄判別DNAマーカーの確立文部科学省：科研費若手B研究期間 : 2013年04月 -2016年03月代表者 : 阿川 泰夫
- 水産生物遺伝マーカー調査 脂質代謝機構解析 水産生物遺伝子発現