詳細

小森　喜久夫 （コモリ　キクオ）

  • 工学部 化学生命工学科　准教授
Last Updated :2024/04/25

コミュニケーション情報 byコメンテータガイド

  • コメント

    生体素子の認識機能を利用した情報・物質・エネルギー変換に関する研究を進めている。バイオアッセイやバイオセンシングなど、医療・環境評価に役立つバイオデバイスへの応用を目指している。

研究者情報

学位

  • 博士（工学）

ホームページURL

J-Global ID

現在の研究分野（キーワード）

    生体素子の認識機能を利用した情報・物質・エネルギー変換に関する研究を進めている。バイオアッセイやバイオセンシングなど、医療・環境評価に役立つバイオデバイスへの応用を目指している。

研究分野

  • ナノテク・材料 / 機能物性化学
  • ナノテク・材料 / 基礎物理化学
  • ライフサイエンス / 生体材料学
  • ライフサイエンス / 生体医工学
  • ものづくり技術（機械・電気電子・化学工学） / バイオ機能応用、バイオプロセス工学

所属学協会

  • 電気化学会   日本化学会   日本動物実験代替法学会   

研究活動情報

論文

  • Yuka Ashitani; Kazutake Takada; Kikuo Komori
    Sensors and Materials 35 9 3201 - 3209 2023年09月 [査読有り]
  • Kikuo Komori; Shinnosuke Takumi; Kiichi Kato; Kazuya Matsumoto; Kohei Shiraishi; Hiroshi Kimura; Kazutake Takada
    Journal of Electroanalytical Chemistry 943 117621 - 117621 2023年08月 [査読有り]
  • Kikuo Komori; Tomoki Aikawa
    Sensors and Actuators Reports 4 100126 - 100126 2022年11月 [査読有り]
  • Dongchen Zhu; Anne-Claire Eiler; Satoshi Ihida; Yasuyuki Sakai; Hiroshi Toshiyoshi; Agnès Tixier-Mita; Kikuo Komori
    IEEJ Transactions on Sensors and Micromachines 142 10 266 - 272 2022年10月 [査読有り]
     
    A transparent high density thin-film-transistor microelectrode array (TFT-µEA) was investigated, for the first time, to apply to real-time electrophysiological monitoring on glucose-stimulated insulin secretion dynamics of pancreatic β cells at higher resolution than conventional microelectrode arrays (MEAs). TFT-µEAs employed in this work are designed based on the switch matrix architecture, which incorporates a large sensing area (15.6 mm × 15.6 mm) with a 150 × 150 array of indium-tin-oxide (ITO) microelectrodes placed at a 100 µm pixel pitch. TFT-µEAs coated with poly-L-lysine and laminin enabled to culture rat insulinoma β line iGL for at least 7 days without cell death, which was determined by conventional cell viability tests based on a fluorescent staining method. Real-time action potentials of iGL cells stimulated by 15 mM glucose were successfully observed in similar to those in a conventional MEAs. These results are the first step towards the development of a multimodal TFT-µEAs device for electrophysiological, biochemical and optical analyses of the pancreatic islets. TFT-µEAs would extremely be promising platforms in the bioanalysis field for neurochemistry and electrophysiology.
  • Kikuo Komori; Ryo Ihara; Seiryu Hirao; Minghao Liu; Yuki Toyota; Mitsutoshi Nakata; Yuta Tani; Kohei Shiraishi
    Journal of Electroanalytical Chemistry 922 116704  2022年10月 [査読有り]
  • Kikuo Komori; Masataka Usui; Kohei Hatano; Yuma Hori; Keita Hirono; Dongchen Zhu; Fumiya Tokito; Masaki Nishikawa; Yasuyuki Sakai; Hiroshi Kimura
    Bioelectrochemistry 143 107972 - 107972 2022年02月 [査読有り]
  • Marie Shinohara; Hiroshi Arakawa; Yuuichi Oda; Nobuaki Shiraki; Shinji Sugiura; Takumi Nishiuchi; Taku Satoh; Keita Iino; Sylvia Leo; Yusuke Kato; Karin Araya; Takumi Kawanishi; Tomoki Nakatsuji; Manami Mitsuta; Kosuke Inamura; Tomomi Goto; Kenta Shinha; Wataru Nihei; Kikuo Komori; Masaki Nishikawa; Shoen Kume; Yukio Kato; Toshiyuki Kanamori; Yasuyuki Sakai; Hiroshi Kimura
    Scientific Reports 11 1 5437  2021年12月 [査読有り]
     
    AbstractExamining intestine–liver interactions is important for achieving the desired physiological drug absorption and metabolism response in in vitro drug tests. Multi-organ microphysiological systems (MPSs) constitute promising tools for evaluating inter-organ interactions in vitro. For coculture on MPSs, normal cells are challenging to use because they require complex maintenance and careful handling. Herein, we demonstrated the potential of coculturing normal cells on MPSs in the evaluation of intestine–liver interactions. To this end, we cocultured human-induced pluripotent stem cell-derived intestinal cells and fresh human hepatocytes which were isolated from PXB mice with medium circulation in a pneumatic-pressure-driven MPS with pipette-friendly liquid-handling options. The cytochrome activity, albumin production, and liver-specific gene expressions in human hepatocytes freshly isolated from a PXB mouse were significantly upregulated via coculture with hiPS-intestinal cells. Our normal cell coculture shows the effects of the interactions between the intestine and liver that may occur in vivo. This study is the first to demonstrate the coculturing of hiPS-intestinal cells and fresh human hepatocytes on an MPS for examining pure inter-organ interactions. Normal-cell coculture using the multi-organ MPS could be pursued to explore unknown physiological mechanisms of inter-organ interactions in vitro and investigate the physiological response of new drugs.
  • Dongchen Zhu; Grant A Cathcart; Satoshi Ihida; Hiroshi Toshiyoshi; Agnès Tixier-Mita; Yasuyuki Sakai; Kikuo Komori
    Journal of Micromechanics and Microengineering 31 11 115002  2021年11月 [査読有り]
  • Anne-Claire Eiler; Pierre-Marie Faure; Junichi Sugita; Satoshi Ihida; Dongchen Zhu; Yasuyuki Sakai; Katsuhito Fujiu; Kikuo Komori; Hiroshi Toshiyoshi; Agnes Tixier-Mita
    IEEE Transactions on Electron Devices 68 4 2041 - 2048 2021年04月 [査読有り]
     
    The constant development and improvement of microelectromechanical systems (MEMS) have been given a great opportunity to develop new reliable microsystems devices for biomedical research. This article presents a locally addressable 2-D arrayed indium-Tin oxide microelectrode platform with integrated thin-film transistors (TFTs) for biological and chemical sensing. Microelectrode arrays (MEAs) using TFT have the advantage of being transparent with a high density of microelectrodes on a large surface. In vitro 2-D electrical measurements on 28 parallel-connected lines selected from a 22 500 MEA were successfully performed with heart cells for the first time. Observation of cell contraction was performed simultaneously with an inverted microscope. Voltammetry and amperometry measurements were also demonstrated using the transparent microelectrodes as working electrodes and an integrated Ag/AgCl reference electrode. Due to its unique features, we believe that the TFT platform can provide more understanding of the key communication between heart cells, and large-scale cardiovascular and nervous systems. As a result, the platform offers unique access to versatile lab-on-A-chip devices that integrate many measurement techniques on one chip for the study of cell cultures, tissues, and organoids.
  • Kikuo Komori; Yuto Komatsu; Masaharu Nakane; Yasuyuki Sakai
    Bioelectrochemistry 138 107719  2021年04月 [査読有り]
  • Biodegradable and Hollowed Micro-Scaffolds for Improved Modular Assembly-Based Tissue Engineering: Design, 3D Fabrication, and Feasibility in Randomly Packed Perfusion Culture
    Y. Pang; S. L; U. Sutoko; Y. Horimoto; K. Montagne; K. Komori; K. Takano; R. Shirakashi; M. Anzai; T. Niino; Y. Sakai
    Biochem. Eng. J. 149 107239  2019年 [査読有り]
  • S. Matsumoto; E. Leclerc; T. Maekawa; H. Kinoshita; M. Shinohara; K. Komori; Y. Sakai; T. Fujii
    Sens. Actuators B 273 1062 - 1069 2018年 [査読有り]
     
    This paper presents a new hepatic culture device, in which oxygen concentration gradient formed by cellular respiration can be visualized as a two-dimensional optical image. An oxygen sensor layer containing Pt (II) octaethylporphyrin (PtOEP) was embedded at the bottom of the device, allowing visualization of oxygen concentration distribution over the cell culture area. Oxygen concentration was determined by analyzing the modulation of fluorescence intensity emitted by the sensor, which is negatively correlated to the concentration. The results in HepG2 cell culture showed that the oxygen gradient and local oxygen partial pressure could be regulated by controlling the perfusion flow rate in the device. The local and physiological oxygen gradient comparable to the in vivo liver lobules can successfully be reproduced in the device. Therefore, periportal and perivenous hepatic like-zones were successfully realized in vitro. The present device would be helpful in the development of novel in vitro methods recapitulating the liver physiology for investigation of hepatic zonation and drug screening system recapitulating the liver physiology.
  • K. Komori; J. Huang; N. Mizushima; S. Ko; T. Tatsuma; Y. Sakai
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS 19 40 27795 - 27800 2017年10月 [査読有り]
     
    Graphene edge sites not only facilitate heterogeneous electron transfer reactions of redox species because of localization of electrons, but also allow sensitivities and selectivities to be tuned by controlling the atomic oxygen/carbon (O/C) ratio. Here, we immobilized fructose dehydrogenase (FDH) onto the surface of cup-stacked carbon nanofibers (CSCNFs), which provide highly ordered graphene edges with a controlled O/C ratio, and investigated the direct electron communication with FDH. As the O/C ratio decreased at the CSCNF surface, the negative zeta potential was mitigated and the electrochemical communication with FDH was facilitated. This is likely due to improved orientation of FDH molecules on the CSCNF surface. CSCNFs with a controlled O/C ratio could be applied to FDH-based D-fructose biosensors with tunable dynamic range and fructose biofuel cells with a controlled maximum current.
  • Rinky Sha; Kikuo Komori; Sushmee Badhulika
    IEEE SENSORS JOURNAL 17 16 5038 - 5043 2017年08月 [査読有り]
     
    Here, we report a grapheme-polyaniline (Gr-PANi) composite modified glassy carbon electrode (GCE) as an amperometric pH sensor. Gr-PANi composite was synthesized by electrodeposition of PANi on the surface of Gr modified GCE using the cyclic voltammetry technique. XRD and Raman analysis confirmed high crystallinity of Gr-PANi composite. FESEM analysis revealed that Gr flakes are surrounded by PANi film because of the heterogeneous nucleation and growth of PANi during electro-polymerization. In acidic region, current of the composite-based pH sensor increased with decreasing pH values of the solution, whereas in alkaline region, current increased with the increasing pH values of the solution. The as-fabricated pH sensor exhibits shorter response time with an excellent sensitivity of -50.14 mu A pH(-1) cm(-2) in the range of pH 1-5 and 139.2 mu A pH(-1) cm(-2) in the range of pH 7-11. The composite-based sensor showed enhanced sensitivity compared with pure Gr-based pH sensor because unlike pristine Gr-based pH sensor, it utilizes both charge-storage mechanisms in EDLCs and pseudo-capacitors, thereby resulting in excellent synergy between Gr and PANi. Reproducibility of the pH sensor was also examined between three different electrodes in the solution of pH 5. Insignificant variation in the current response suggested excellent reproducibility of pH sensor. To the best of our knowledge, no other studies have been reported on the Gr-PANi composite-based pH sensor over wide pH range. This Gr-PANi-based pH sensor offers a simple, low-cost, promising, real-time amperometric pH sensor in chemistry, clinical diagnostics, bio-sensing, and environmental monitoring applications.
  • Marie Shinohara; Kikuo Komori; Teruo Fujii; Yasuyuki Sakai
    Biomedical Physics and Engineering Express 3 4 2017年07月 [査読有り]
     
    Three-dimensional hepatocyte aggregates possessing highly-metabolic functions are required for analytical applications, such as in drug discovery and drug toxicity screening, to obtain physiologically relevant outcomes. Here, we report a rapid and highly productive method to obtain size-controlled rat hepatocyte aggregates using oxygen permeable honeycomb microwells. Rapid cell aggregation occurred in honeycomb microwells even with increased cell density per unit area (four times higher than the cell density in a conventional polystyrene culture dish). The activity of cytochrome P450 (CYP) 1A1 and CYP3A2 was enhanced in the hepatocyte aggregates, 52-88 μmin diameter, compared with those in the hepatocyte monolayer. These findings are expected to contribute to the rapid organization of optimal-sized hepatocyte aggregates for cell-based assays.
  • Rinky Sha; Kikuo Komori; Sushmee Badhulika
    ELECTROCHIMICA ACTA 233 44 - 51 2017年04月 [査読有り]
     
    Detection of urea is of prime importance in food and water safety, dairy industries and environmental monitoring. Traditional methods to detect urea are either expensive and involve sophisticated instrumentation or are based on enzymatic approach of detection. Herein, we report a Graphene-Polyaniline (Gr-PANi) based electrochemical sensor for non-enzymatic detection of urea. Gr-PANi composite was synthesized by electro-deposition of PANi on the surface of Gr modified GCE using cyclic voltammetry (CV) technique. The presence of Gr and PANi in the composite was confirmed using a multitude of characterization techniques which included FESEM, XRD and Raman spectroscopy. The electrochemical behavior of urea at the surface of Gr-PANi modified GCE was studied by CV whereas urea sensing was performed by using simple current-potential (I-V) technique. The current response of the as fabricated urea sensor was similar to 4.74 folds greater than that of pure PANi based sensor and similar to 67.2 times greater than that of pure Gr based sensor. The sensing performance of the composite based urea sensor was optimized by varying the thickness of PANi film. The optimized sensor exhibited lower limit of detection (5.88 mu M), excellent reproducibility, selectivity and stability with an enhanced sensitivity of 226.9 mu A/mu M cm(2) (R-2 = 0.993) in the range of 10 mu M-200 mu M. The reliability of the as-fabricated sensor was successfully investigated by using it to detect urea concentrations in samples of tap water and milk samples. This highly-sensitive Gr-PANi composite based urea sensor provides a simple, low cost, non enzymatic approach for detection of urea that find numerous applications in clinical diagnostics, dairy industries, fertilizer plants and environmental monitoring. (C) 2017 Elsevier Ltd. All rights reserved.
  • Kikuo Komori; Trupti Terse-Thakoor; Ashok Mulchandani
    BIOELECTROCHEMISTRY 111 57 - 61 2016年10月 [査読有り]
     
    Horseradish peroxidase (HRP) was immobilized through sodium dodecyl sulfate (SDS) on the surface of a seamless three-dimensional hybrid of carbon nanotubes grown at the graphene surface (HRP-SDS/CNTs/G) and its electrochemical properties were investigated. Compared with graphene alone electrode modified with HRP via SDS (HRP-SDS/G electrode), the surface coverage of electroactive HRP at the CNTs/G electrode surface was approximately 2-fold greater because of CNTs grown at the graphene surface. Based on the increase in the surface coverage of electroactive HRP, the sensitivity to H2O2 at the HRP-SDS/CNTs/G electrode was higher than that at the HRP-SDS/G electrode. The kinetics of the direct electron transfer from the CNTs/G electrode to compound I and II of modified HRP was also analyzed. (C) 2016 Elsevier B.V. All rights reserved.
  • Kikuo Komori; Tetsu Tatsuma; Yasuyuki Sakai
    LANGMUIR 32 36 9163 - 9170 2016年09月 [査読有り]
     
    Electron transfer kinetics at the graphene edge site is of great interest from the viewpoints of application to sensing and energy conversion and storage. Here we analyzed kinetics of direct electron transfer of horseradish. peroxidase (HRP) adsorbed through surfactant sodium dodecyl sulfate,at cup-stacked carbon nanofibers (CSCNFs), which provide highly ordered graphene edges, and compared it with that at single-walled carbon nanotube (SWCNTs), which consist of a rolled-up basal plane graphene. The heterogeneous electron transfer rate constant of the Fe2+/(3+) couple of the HRP reaction center at CSCNFs (ca. 34.8 s(-1)) was an order of magnitude larger than that at SWCNTs (ca. 4.7 s(-1)). In addition, the overall rate constant of the electron transfer reaction from CSCNFs to HRP oxidized by H2O2 was higher than that from SWCNTs by a factor of 3. CSCNFs also allowed enhancement of the complex-formation reaction rate of HRP with H2O2, in comparison with that at SWCNTs. CSCNFs would therefore be applied to not only biosensors but also biofuel cells with enhanced performance.
  • Y. Pang; Y. Horimoto; S. Sutoko; K. Montagne; M. Shinohara; D. Mathiue; K. Komori; M. Anzai; T. Niino; Yasuyuki Sakai
    BIOFABRICATION 8 3 2016年09月 [査読有り]
     
    A novel engineering methodology for organizing a large liver tissue equivalent was established by intergrating both 'top down' and 'bottom up' approaches. A three-dimensional (3D) scaffold was engineered comprising 43 culture chambers (volume: 11.63 cm(3)) assembled in a symmetrical pattern on 3 layers, a design which enables further scaling up of the device to a clinically significant size (volume: 500 cm(3)). In addition, an inter-connected flow channel network was designed and proved to homogenously deliver culture medium to each chamber with the same pressure drop. After fabrication using nylon-12 and a selective laser sintering process, co-cultured cellular aggregates of human hepatoma Hep G2 and TMNK-1 cells were loosely packed into the culture chambers with biodegradable poly-L-lactic acid fibre pieces for 9 days of perfusion culture. The device enabled increased hepatic function and well-maintained cell viability, demonstrating the importance of an independent medium flow supply for cell growth and function provided by the current 3D scaffold. This integrative methodology from the macro- to the micro-scale provides an efficient way of arranging engineered liver tissue with improved mass transfer, making it possible to further scale up to a construct with clinically relevant size while maintaining high per-volume-based physiological function in the near future.
  • Kikuo Komori; Kentaro Yamura; Atsushi Kogo; Yusuke Takahashi; Tetsu Tatsuma; Akiyoshi Sakoda; Yasuyuki Sakai
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 89 5 603 - 607 2016年05月 [査読有り]
     
    Oxygenated cup-stacked carbon nanofibers (ox-CSCNFs), the surface of which provides highly ordered graphene edges and oxygen-containing functional groups, were introduced to a nanoporous TiO2 photoelectrode for enhanced photocatalytic currents. The ox-CSCNFs are characterized by good electric conductivity and high dispersibility in solvents. Under UV irradiation, short-circuit photocurrents based on water oxidation at the photoelectrode and oxygen reduction at a counter electrode were enhanced by about 15 times by introduction of the ox-CSCNFs. The oxygen-containing functional groups, which may facilitate good contact between the ox-CSCNFs and TiO2, are essential for the dramatic enhancement in the photocurrent. Recombination efficiency between excited electrons and generated holes may be reduced by facilitated electron transport from excited TiO2 to a substrate electrode via ox-CSCNFs. Photo currents for photocatalytic oxidation of glucose were also enhanced by about 20-fold. The present composite materials would be applied to photocatalytic and photovoltaic cells with enhanced performances.
  • Trupti Terse-Thakoor; Kikuo Komori; Pankaj Ramnani; Ilkeun Lee; Ashok Muchandani
    LANGMUIR 31 47 13054 - 13061 2015年12月 [査読有り]
     
    Three-dimensional seamless chemical vapor deposition (CVD) grown graphene-carbon nanotubes (G-CNT) hybrid film has been studied for its potential in achieving direct electron transfer (DET) of glucose oxidase (GOx) and its bioelectrocatalytic activity in glucose detection. A two-step CVD method was employed for the synthesis of seamless G-CNT hybrid film where CNTs are grown on already grown graphene film on copper foil using iron as a catalyst. Physical characterization using SEM and TEM show uniform dense coverage of multiwall carbon nanotubes (MWCNT) grown directly on graphene with seamless contacts. The G-CNT hybrid film was electrochemically modified to introduce oxygenated functional groups for DET favorable immobilization of GOx. Pristine and electrochemically functionalized G-CNT film was characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, X-ray photoelectron-spectroscopy, and Raman spectroscopy. The DET between GOx and electrochemically oxidized G-CNT electrode was studied using cyclic voltammetry which showed a pair of well-defined and quasi-reversible redox peaks with a formal potential of -459 mV at pH 7 corresponding to the redox site of GOx. The constructed electrode detected glucose concentration over the clinically relevant range of 2-8 mM with the highest sensitivity of 19.31 mu A/mM/cm(2) compared to reported composite hybrid electrodes of graphene oxide and CNTs. Electrochemically functionalized CVD grown seamless G-CNT structure used in this work has potential to be used for development of artificial mediatorless redox enzyme based biosensors and biofuel cells.
  • Kikuo Komori; Trupti Terse-Thakoor; Ashok Mulchandani
    ACS APPLIED MATERIALS & INTERFACES 7 6 3647 - 3654 2015年02月 [査読有り]
     
    A seamless three-dimensional hybrid film consisting of carbon nanotubes grown at the graphene surface (CNTs/G) is a promising material for the application to highly sensitive enzyme-based electrochemical biosensors. The CNTs/G film was used as a conductive nanoscaffold for enzymes. The heme peptide (HP) was immobilized on the surface of the CNTs/G film for amperometric sensing of H2O2. Compared with flat graphene electrodes modified with HP, the catalytic current for H2O2 reduction at the HP-modified CNTs/G electrode increased due to the increase in the surface coverage of HP. In addition, microvoids in the CNTs/G film contributed to diffusion of H2O2 to modified HP, resulting in the enhancement of the catalytic cathodic currents. The kinetics of the direct electron transfer from the CNTs/G electrode to compound I and II of modified HP was also analyzed.
  • Wenjin Xiao; Guillaume Perry; Kikuo Komori; Yasuyuki Sakai
    INTEGRATIVE BIOLOGY 7 11 1412 - 1422 2015年 [査読有り]
     
    To develop an in vitro liver tissue equivalent, hepatocytes should be cocultured with liver non-parenchymal cells to mimic the in vivo physiological microenvironments. In this work, we describe a physiologically-relevant liver tissue model by hierarchically organizing layers of primary rat hepatocytes and human liver sinusoidal endothelial cells (TMNK-1) on an oxygen-permeable polydimethylsiloxane (PDMS) membrane, which facilitates direct oxygenation by diffusion through the membrane. This in vivo-mimicking hierarchical coculture was obtained by simply proceeding the overlay of TMNK-1 cells on the hepatocyte layer re-formed on the collagen immobilized PDMS membranes. The comparison of hepatic functionalities was achieved between coculture and sandwich culture with Matrigel, in the presence and absence of direct oxygenation. A complete double-layered structure of functional liver cells with vertical contact between hepatocytes and TMNK-1 was successfully constructed in the coculture with direct oxygen supply and was well-maintained for 14 days. The hepatocytes in this hierarchical culture exhibited improved survival, functional bile canaliculi formation, cellular level polarization and maintenance of metabolic activities including Cyp1A1/2 activity and albumin production. By contrast, the two cell populations formed discontinuous monolayers on the same surfaces in the non-oxygen-permeable cultures. These results demonstrate that (i) the direct oxygenation through the PDMS membranes enables very simple formation of a hierarchical structure consisting of a hepatocyte layer and a layer of TMNK-1 and (ii) we may include other non-parenchymal cells in this format easily, which can be widely applicable to other epithelial organs.
  • Wenjin Xiao; Marie Shinohara; Kikuo Komori; Yasuyuki Sakai; Hitoshi Matsui; Tomoharu Osada
    BIOTECHNOLOGY PROGRESS 30 6 1401 - 1410 2014年11月 [査読有り]
     
    Oxygen supply is a critical issue in the optimization of in vitro hepatocyte microenvironments. Although several strategies have been developed to balance complex oxygen requirements, these techniques are not able to accurately meet the cellular oxygen demand. Indeed, neither the actual oxygen concentration encountered by cells nor the cellular oxygen consumption rates (OCR) was assessed. The aim of this study is to define appropriate oxygen conditions at the cell level that could accurately match the OCR and allow hepatocytes to maintain liver specific functions in a normoxic environment. Matrigel overlaid rat hepatocytes were cultured on the polydimethylsiloxane (PDMS) membranes under either atmospheric oxygen concentration [20%-O-2 (+)] or physiological oxygen concentrations [10%-O-2 (+), 5%-O-2 (+)], respectively, to investigate the effects of various oxygen concentrations on the efficient functioning of hepatocytes. In parallel, the gas-impermeable cultures (polystyrene) with PDMS membrane inserts were used as the control groups [PS-O-2 (-)]. The results indicated that the hepatocytes under 10%-O-2 (+) exhibited improved survival and maintenance of metabolic activities and functional polarization. The dramatic elevation of cellular OCR up to the in vivo liver rate proposed a normoxic environment for hepatocytes, especially when comparing with PS-O-2 (-) cultures, in which the cells generally tolerated hypoxia. Additionally, the expression levels of 84 drug-metabolism genes were the closest to physiological levels. In conclusion, this study clearly shows the benefit of long-term culture of hepatocytes at physiological oxygen concentration, and indicates on an oxygen-permeable membrane system to provide a simple method for in vitro studies. (c) 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:1401-1410, 2014
  • Wenjin Xiao; Makoto Kodama; Kikuo Komori; Yasuyuki Sakai
    BIOCHEMICAL ENGINEERING JOURNAL 91 99 - 109 2014年10月 [査読有り]
     
    Although there have been remarkable progresses in hepatocytes cultures in terms of mimicking microenvironments of in vivo liver, oxygen supply is still a critical issue. In this study, we investigated the effect of direct oxygenation through oxygen-permeable membranes on functionalities of hepatocytes in two widely accepted advanced culture models, sandwich culture and 3D culture. Rat hepatocytes were cultured on the polydimethylsiloxane (PDMS) membranes for 14 days in monolayer culture, sandwich culture with Matrigel and 3D culture with microporous expanded polytetrafluoroethylene (ePTFE) membranes in the presence and absence of direct oxygenation from the other side of the membranes. The present results showed remarkable enhancement of hepatocytes duration and their functions by oxygen transfer through PDMS membranes in all these three cultures. The hepatocytes cultured in sandwich with oxygen exhibited extended survival and highest maintenance of metabolic activities, such as albumin productivity and Cyp1 a1/2 activity. Additionally, the expression levels of various drug-metabolism genes, as examined by PCR arrays, were also closest to those of freshly isolated hepatocytes. As the cellular maintenance has been greatly improved by microporous ePTFE membranes, the hepatocytes in 3D culture performed increased functions that comparable to those in sandwich culture. This study clearly illustrates that oxygenation is a critical factor to be considered in optimization of the microenvironments of hepatocytes cultures. (C) 2014 Elsevier B.V. All rights reserved.
  • Seongjae Ko; Tetsu Tatsuma; Akiyoshi Sakoda; Yasuyuki Sakai; Kikuo Komori
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS 16 24 12209 - 12213 2014年 [査読有り]
     
    Oxygenated cup-stacked carbon nanofibers (CSCNFs), the surface of which provides highly ordered graphene edges and oxygen-containing functional groups, were investigated as electrode materials by using typical redox species in electrochemistry, Fe2+/(3+), [Fe(CN)(6)] (3-)/(4-), and dopamine. The electron transfer rates for these redox species at oxygenated CSCNF electrodes were higher than those at edge-oriented pyrolytic graphite and glassy carbon electrodes. In addition, the oxygen-containing functional groups also contributed to the electron transfer kinetics at the oxygenated CSCNF surface. The electron transfer rate of Fe2+/(3+) was accelerated and that of [Fe(CN)(6)] (3-)/(4-) was decelerated by the oxygen-containing groups, mainly due to the electrostatic attraction and repulsion, respectively. The electrochemical reaction selectivities at the oxygenated CSCNF surface were tunable by controlling the amount of nanofibers and the oxygen/carbon atomic ratio at the nanofiber surface. Thus, the oxygenated CSCNFs would be useful electrode materials for energy-conversion, biosensing, and other electrochemical devices.
  • M. Shinohara; H. Kimura; K. Montagne; K. Komori; T. Fujii; Y. Sakai
    Biotechnol. Prog. 30 1 178 - 187 2014年01月 [査読有り]
     
    Spherical three-dimensional (3D) cellular aggregates are valuable for various applications such as regenerative medicine or cell-based assays due to their stable and high functionality. However, previous methods to form aggregates have shown drawbacks, being labor-intensive, showing low productivity per unit area or volume and difficulty to form homogeneous aggregates. We proposed a novel strategy based on oxygen-permeable polydimethylsiloxane (PDMS) honeycomb microwell sheets, which can theoretically supply about 80 times as much oxygen as conventional polystyrene culture dishes, to produce recoverable aggregates in controllable sizes using mouse insulinoma cells (MIN6-m9). In 48 hours of culture, the PDMS sheets produced aggregates whose diameters were strictly controlled (?32, 60, 90, 150 and 280 mm) even at an inoculum density eight times higher (8.0x105 cells/cm(2)) than that of normal confluent monolayers (1.0x105 cells/cm(2)). Measurement of the oxygen tension near the cell layer and glucose/lactate analysis clearly showed that cells exhibit aerobic respiration on the PDMS-based culture system. Glucose-responsive insulin secretion of the recovered aggregates showed that the aggregates around 90 mm in diameter secreted the largest amounts of insulin. This confirmed the advantages of 3D cellular organization and the existence of a suitable aggregate size, above which excess organization leads to a decreased metabolic response. These results demonstrated that this microwell-based PDMS culture system provides a promising method to form size-regulated and better functioning 3D cellular aggregates of various kinds of cells with a high yield per surface area. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:178-187, 2014
  • K. Komori; M. Udagawa; M. Shinohara; K. Montagne; T. Tsuru; Y. Sakai
    Biomater. Sci. 1 5 510 - 518 2013年05月 [査読有り]
     
    Producing sheet-like tissues is a promising strategy for implantable engineered tissues, because in vitro pre-vascularization is dispensable in this configuration. We developed a simple methodology for the formation and non-destructive harvesting of a thick pancreatic β-cell sheet consisting of mouse insulinoma MIN6-m9 cells and mouse NIH3T3 fibroblasts using an O 2-permeable polydimethylsiloxane plate modified with poly(N-isopropylacrylamide) (O2+/PNIPA-PDMS plate). Owing to the direct oxygenation of the cells through the PNIPA-modified PDMS plate, a viable, metabolically active sheet 5-6 cell layers thick (ca. 60 μm thick) was formed spontaneously in the absence of direct oxygenation, only a thin cell sheet could be formed consisting of at most 2 layers (ca. 20 μm thick) with mainly anaerobic metabolism. Consequently, the net density of MIN6-m9 cells under direct oxygenation was about twice as high as in the absence of direct oxygenation. Accordingly, the insulin secretion for 10 to 60 min after glucose stimulation was also about 1.5 times higher with oxygenation. Furthermore, the thick cell sheet was successfully harvested from the O2+/PNIPA-PDMS plate surface in a non-destructive manner by inducing a phase transition of PNIPA by lowering the temperature below the lower critical solution temperature. Thus, the present report shows a promising and simple method to produce thick sheet-like engineered tissues for transplantation that could be used as a treatment for type 1 diabetes. © 2013 The Royal Society of Chemistry.
  • K. Iwasawa; G. Tanaka; T. Aoyama; M. M. Chowdhury; K. Komori; T. Tanaka-Kagawa; H. Jinno; Y. Sakai
    AATEX 18 19 - 31 2013年 [査読有り]
  • Y. Pang; K. Montagne; M. Shinohara; K. Komori; Y. Sakai
    BIOFABRICATION 4 4 2012年12月 [査読有り]
     
    To realize long-term in vitro culture of hepatocytes at a high density while maintaining a high hepatic function for aggregate-based liver tissue engineering, we report here a novel culture method whereby endothelialized rat hepatocyte aggregates were formed using a PDMS microwell device and cultured in a perfusion bioreactor by introducing spacers between aggregates to improve oxygen and nutrient supply. Primary rat hepatocyte aggregates around 100 mu m in diameter coated with human umbilical vein endothelial cells were spontaneously and quickly formed after 12 h of incubation, thanks to the continuous supply of oxygen by diffusion through the PDMS honeycomb microwell device. Then, the recovered endothelialized rat hepatocyte aggregates were mixed with biodegradable poly-L-lactic acid fibres in suspension and packed into a PDMS-based bioreactor. Perfusion culture of 7 days was successfully achieved with more than 73.8% cells retained in the bioreactor. As expected, the fibres acted as spacers between aggregates, which was evidenced from the enhanced albumin production and more spherical morphology compared with fibre-free packing. In summary, this study shows the advantages of using PDMS-based microwells to form heterotypic aggregates and also demonstrates the feasibility of spacing tissue elements for improving oxygen and nutrient supply to tissue engineering based on modular assembly.
  • Seongjae Ko; Yusuke Takahashi; Akiyoshi Sakoda; Yasuyuki Sakai; Kikuo Komori
    LANGMUIR 28 23 8760 - 8766 2012年06月 [査読有り]
     
    Uniformly sized microspheres tangled with cup-stacked carbon nanofibers (CSCNFs) were directly synthesized by the pyrolysis of poly(ethylene glycol) (PEG) with a nickel catalyst. A PEG/Ni membrane was prepared on a silicon wafer surface by heating it to 750 degrees C at a heating rate of 15 degrees C min(-1). The wafer was heated to a temperature of 400 degrees C and was held at that temperature for 1 h before raising the temperature to 750 degrees C for 10 min to form the CSCNF microspheres. The final CSCNF microspheres and the intermediates were evaluated using scanning electron microscopy, transmission electron microscopy, X-ray diffractometry, and Raman spectroscopy to elucidate the growth mechanism. Furthermore, the CSCNF microspheres were successfully dispersed and maintained their spherical shape in an aqueous solution containing 0.5% Nafion. The CSCNF microspheres have the potential to work as a sophisticated carrier with high adsorption and fast electron-transfer exchange properties based on the graphene edges of the nanofiber surface.
  • Kikuo Komori; Sho Fujii; Kevin Montagne; Hiroko Nakamura; Hiroshi Kimura; Katsuto Otake; Teruo Fujii; Yasuyuki Sakai
    SENSORS AND ACTUATORS B-CHEMICAL 162 1 278 - 283 2012年02月 [査読有り]
     
    In this study, we developed a well-of-the-well (WOW) system-based embryo culture plate with an oxygen sensing photoluminescent probe (OSPP). This system can incubate single embryos in an array of microwells and continuously and non-invasively monitor their morphology by microscopy and respiration metabolic activity based on the quenching of photoexcited platinum octaethylporphyrin by oxygen. Although the development rate to the blastocyst stage obtained in the WOW embryo culture plate with the OSPP was about 11% lower than that obtained with the conventional drop culture method because of the absence of paracrine effects due to the size of the microwells, the average cell number in the embryo at the blastocyst stage was not significantly different between the two culture systems. However, the present embryo culture plate enabled the selection and harvest of high quality blastocysts by monitoring both their morphology and their oxygen consumption rate, making it suitable for the high throughput evaluation of embryos. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
  • Seongjae Ko; Yusuke Takahashi; Hirotaka Fujita; Tetsu Tatsuma; Akiyoshi Sakoda; Kikuo Komori
    RSC ADVANCES 2 4 1444 - 1449 2012年 [査読有り]
     
    Cup-stacked carbon nanofibers (CSCNFs) were treated with ozone to introduce oxygen-containing groups to the edges of the graphene cups. The O/C atomic ratio at the surface was estimated to be 5.3 x 10(-2). The hydrophilic CSCNFs were used for constructing a three-dimensional network that works both as an electrical nanowire and an enzyme support. Horseradish peroxidase (HRP) molecules were immobilized onto the network for amperometric sensing of H2O2 and inhibition-based sensing of cyanide. Both the upper sensing limit of H2O2 and the upper and lower sensing limits of cyanide were controlled by about two orders of magnitude by changing the HRP coverage.
  • Naoko Yamamoto; Kikuo Komori; Kevin Montagne; Hitoshi Matsui; Hidenari Nakayama; Shoji Takeuchi; Yasuyuki Sakai
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING 111 4 454 - 458 2011年04月 [査読有り]
     
    We present an improved cytotoxicity test for reactive metabolites, in which the 59 microsomal fraction of rat liver homogenate is encapsulated in alginate gel microbeads to avoid cytotoxic effects of 59-self-generated toxicants, microsomal lipid peroxides. The 59-encapsulated gel microbeads were prepared by a coaxial two-fluid nozzle and surfaces of the microbeads were coated with poly-L-lysine (PLL). Although the initial metabolic rate of the 59-encapsulated gel microbeads was about 20% slower than that of bare 59, the microbeads prevented the leakage of microsomal lipid peroxides thanks to the dense alginate and PLL polymer networks. In fact, the half maximal effective concentration of the indirect mutagen cyclophosphamide on NIH3T3 cells in the presence of the 59-encapsulated gel microbeads was about 5 times higher than that in the presence of bare S9. Use of the S9-encapsulated gel microbeads enabled the more accurate evaluation of the cytotoxicity of the reactive metabolites without the S9-based cytotoxicity. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
  • Kazuyuki Ishii; Kensuke Kubo; Tomoya Sakurada; Kikuo Komori; Yasuyuki Sakai
    CHEMICAL COMMUNICATIONS 47 17 4932 - 4934 2011年 [査読有り]
     
    We have applied phthalocyaninatosilicon (SiPc) covalently linked to one or two tetramethyl-1-piperidinyloxyl (TEMPO) radicals as fluorescence probes for detecting ascorbic acid in biological systems.
  • Qi Rui; Kikuo Komori; Yang Tian; Haiqing Liu; Yongping Luo; Yasuyuki Sakai
    ANALYTICA CHIMICA ACTA 670 1-2 57 - 62 2010年06月 [査読有り]
     
    In this work, direct electron transfer of cytochrome c (cyt. c)-a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E-0') of cyt. c is estimated to be 338.2 +/- 4.3 mV (vs. Ag vertical bar AgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0V (vs. Ag vertical bar AgCl), the electrochemical determination of H2O2 is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O-2. Such an excellent selectivity enable the present H2O2 biosensor determine the extracellular H2O2 released from living human hepatoma cells. (C) 2010 Elsevier B.V. All rights reserved.
  • H. Kimura; H. Takeyama; K. Komori; T. Yamamoto; Y. Sakai; T. Fujii
    J. Robotics Mech. 22 594 - 600 2010年 [査読有り]
  • N. Kojima; K. Miura; T. Matsuo; H. Nakayama; K. Komori; S. Takeuchi; Y. Sakai
    J. Robotics Mech. 22 5 619 - 622 2010年 [査読有り]
  • Kikuo Komori; Shotaro Miyajima; Tatsuro Tsuru; Takao Fujii; Shino Mohri; Yoshiro Ono; Yasuyuki Sakai
    CHEMOSPHERE 77 8 1106 - 1112 2009年11月 [査読有り]
     
    We have developed a rapid and simple gas toxicity evaluation system based on bioluminescence inhibition of a marine-derived wild luminous bacterium, Vibrio fischeri. The luminous bacteria were trapped using a thin polyion complex membrane in order to allow semi direct contact between the bacteria and toxic gases. Bioluminescence inhibition ratios of the present system to six reference gases, including benzene, trichloroethylene. acetone, NO2. SO2, and CO, were evaluated, and dose-response relationships were successfully obtained after 15 min of gas exposure, except for CO gas. The sensitivity to the five gases except for CO gas of the present system was 1-3 orders of magnitude higher than that in acute animal tests. The present system also allowed for the evaluation of overall toxicity of some environmental gases containing various chemicals. These results clearly demonstrated that the present system would be a valuable prototype for rapid and on-site acute toxicity detection of a gas mixture, such as environmental gases. (C) 2009 Elsevier Ltd. All rights reserved.
  • Kikuo Komori; Jun Nada; Masaki Nishikawa; Hideo Notsu; Tetsu Tatsuma; Yasuyuki Sakai
    ANALYTICA CHIMICA ACTA 653 2 222 - 227 2009年10月 [査読有り]
     
    A prototype of a mammalian cell array chip was developed on a flat glass surface. A superhydrophilic (water contact angle=5 degrees)/highly hydrophobic (120 degrees) pattern was prepared on a fluorinated polymer-coated glass surface by means of photocatalytic lithography, and A549 (a human alveolar epithelial cell line), Hep G2 (a human hepatoma cell line) and mouse fibroblast 3T3 cells were inoculated onto the superhydrophilic regions. The cell populations were confined in the superhydrophilic regions for at least 24h and separated from each other for at least one week. Organ-specific toxicity of aflatoxin B(1) and non-specific toxicity of adriamycin were successfully detected by using the cell array chip. (c) 2009 Elsevier B.V. All rights reserved.
  • Kikuo Komori; Sumi Takamiya; Nobuhiko Kojima; Osamu Kuwazuru; Nobuhiro Yoshikawa; Katsuko Furukawa; Kenichi Miyamoto; Yasuyuki Sakai
    CHEMISTRY LETTERS 38 9 878 - 879 2009年09月 [査読有り]
     
    A reconstituted porous elastin film prepared by a novel cross-linker, Dode-DSP. was examined for its feasibility as a biocompatible and elastic cellular scaffold. Although the mechanical properties of the film alone drastically deteriorated in physical conditions due to degradation, loading of fibroblasts retarded the deterioration in terms of distensibility and E value (Young's modulus).
  • Kevin Montagne; Kikuo Komori; Fei Yang; Tetsu Tatsuma; Teruo Fujii; Yasuyuki Sakai
    PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 8 11 1529 - 1533 2009年 [査読有り]
     
    We propose a simple method for producing micropatterned cell spots by photocatalytic lithography on a Pt porphyrin-based oxygen-sensitive polystyrene membrane that enables real-time imaging of oxygen consumption of patterned cell spots with sub-millimetre resolution.
  • Kikuo Komori; Kenji Murai; Shotaro Miyajima; Takao Fujii; Shino Mohri; Yoshiro Ono; Yasuyki Sakai
    ANALYTICAL SCIENCES 24 8 957 - 962 2008年08月 [査読有り]
     
    To simply evaluate toxicity for various types of exhaust-gas samples collected in various locations, we developed a small-scale (150 mL) batch-type completely closed gas exposure device incorporated with an air-liquid interface culture of a human alveolar epithelial cell line, A549. On the basis of cell viability tests using an acid phosphatase assay after 48 h of gas exposure, the developed device was able to measure clear dose-response relationships for volatile organic and inorganic compounds, such as benzene, trichloroethylene (TCE), acetone, SO2 and NO2 gases, but not CO gas. Although the 50% effective concentration values in the device were much higher than 50% lethal concentration values reported in animal experiments, the tendency of the toxic intensity observed in the former was roughly consistent with that of the acute toxicity in the latter. We further applied the device to evaluate the toxicity of cigarette smoke as an example of actual environmental gases, and successfully measured acute cell death from the gas after 48 h of exposure. The present small device is expected to be one of good tools not only in simultaneously assessing various gaseous chemicals or samples, but also in studying acute toxicity expression mechanisms in human lung epithelia.
  • Masaki Nishikawa; Takatoki Yamamoto; Nobuhiko Kojima; Komori Kikuo; Teruo Fujii; Yasuyuki Sakai
    BIOTECHNOLOGY AND BIOENGINEERING 99 6 1472 - 1481 2008年04月 [査読有り]
     
    The highly oxygen-permeable material, polydimethylsiloxane (PDMS), has the potential to be applied to cell culture microdevices,"but cell detachment from PDMS has been a major problem. In this study, we demonstrate that a combination of collagen covalently immobilized PDMS and an adequate oxygen supply enables the establishment of a stable, attached spheroid (hemispheroid) culture of rat hepatocytes. The bottom PDMS surfaces were first treated with oxygen plasma, then coupled with aminosilane followed by a pbotoreactive crosslinker, and they were finally reacted with a collagen solution. X-ray photoelectron spectroscopy (XPS) and contact angle measurements showed that the covalent immobilization of collagen on the surface occurred only where the crosslinker had been introduced. On the collagen-conjugated PDMS surface, rat hepatocytes organized themselves into hemispheroids and maintained the viability and a remarkably high albumin production at least for 2 weeks of culture. In contrast, hepatocytes on the other types of PDMS surfaces formed suspended spheroids that had low albumin production. In addition, we showed that blocking the oxygen supply through the bottom PDMS surface inhibited the formation of hemispheroids and the augmentation of hepatocellular function. These results show that appropriate surface modification of PDMS is a promising approach towards the development of liver tissue microdevices.
  • Masaki Nishikawaa; Nobuhiko Kojimaa; Kikuo Komori; Takatoki Yamamoto; Teruo Fujii; Yasuyuki Sakai
    JOURNAL OF BIOTECHNOLOGY 133 2 253 - 260 2008年01月 [査読有り]
     
    In in vivo liver tissue, each hepatocyte has intimate interactions not only with adjacent hepatocytes but also with nonparenchymal cells in a three-dimensional (3D) manner. We recently reported that hepatic function is highly maintained on collagen covalently immobilized polydimethylsiloxane (PDMS) membranes through which oxygen is supplied directly to the cells. In this study, to further enhance performances of hepatocytes culture, we investigated cocultivation of rat hepatocytes with a mouse fibroblast, NIH/3T3 (M) in the same PDMS membranes. Various functions of hepatocytes were better maintained on the membrane at remarkably higher levels, particularly albumin secretion on such coculture was about 20 times higher than that in conventional coculture on tissue-culture-treated polystyrene (TCPS) surfaces. The remarkable functional enhancements are likely to be explained by the net growth of hepatocytes (from 1.2- to 1.4-fold inoculated number) and very intimate contact between hepatocytes and 3T3 cells in almost continuous double-layered structures under the adequate oxygen supply. The results demonstrate that simultaneous realization of different requirements toward mimicking in vivo liver tissue microstructure is effective in improving performance of hepatocytes culture system. (c) 2007 Elsevier B.V. All rights reserved.
  • Kikuo Komori; Kazutake Takada; Osamu Hatozaki; Noboru Oyama
    LANGMUIR 23 11 6446 - 6452 2007年05月 [査読有り]
     
    The electrochemiluminescence (ECL) of magnetic microbeads modified with tris(2,2'-bipyridine)ruthenium(II) ([Ru(bpy)(3)](2+)) was studied in the presence of tri-n-propylamine (TPA) to develop highly sensitive ECL detection system, where the employed microbead has a diameter of 4.5 mu m. The ECL signal of the [Ru(bpy)(3)](2+) derivative-modified magnetic microbeads was found to be affected by the geometrical distribution of the magnetic microbeads on the electrode surface. The ECL peak intensity increased with increasing the number of the beads on the electrode surfaces up to 1.6 x 10(6) beads cm(-2), although above 1.6 x 10(6) beads cm(-2), it decreased. The ECL decrease arises from the physical prevention of the ECL from reaching the photomultiplier tube by the excessive beads. The observed peak ECL signal of the [Ru(bpy)(3)](2+) derivative-modified magnetic microbeads in the presence of NaN3, which serves as a preservative substance, mainly appeared at a potential of +0.90 V vs Ag/AgCl where [Ru(bpy)(3)](2+) is hardly oxidized, whereas the ECL signal in the absence of NaN3 appeared at a potential of +1.15 V. The presence of NaN3 on the electrode surface retards formation of an oxide layer on the electrode surfaces and promotes TPA oxidation. The ECL response at +0.90 V was mainly attributed to ECL reaction of excited-state [Ru(bpy)(3)](2+*) formed by oxidation of [Ru(bpy)(3)](+) with TPA radical cation, where the [Ru(bpy)(3)](+) was generated by reduction of [Ru(bpy)(3)](2+) with TPA radical.
  • Development of a Multi-Compartment Micro-Cell Culture Device as a Future on-Chip Human: Fabrication of a Three-Compartment Device and Immobilization of Rat Mature Adipocytes for the Evaluation of Chemical Distributions
    H. Nakayama; H. Kimura; K. Komori; T. Fujii; Y. Sakai
    J. Robotics Mech. 19 544 - 549 2007年 [査読有り]
  • K Komori; K Takada; T Tatsuma
    LANGMUIR 22 1 478 - 483 2006年01月 [査読有り]
     
    An electrode was modified with a phase transition polymer, poly(N-isopropylacrylamide), and the polymer was further modified with a peroxidase model compound, heme peptide (HP). As the polymer layer shrank at temperatures above 30-40 degrees C, the catalytic activity of the HP molecules for H2O2 reduction improved, and simultaneously, the number of HP molecules that can communicate electrochemically with the electrode increased. As a result, the catalytic current for H2O2 reduction in the shrunken state was 4 times larger than that in the swollen state. This reversible change was exploited for tuning the sensitivity and dynamic range of the HP electrode in H2O2 biosensing. The dynamic range in inhibition-based biosensing of imidazole derivatives was also tunable.
  • Kikuo Komori; Kazutake Takada; Tetsu Tatsuma
    Journal of Electroanalytical Chemistry 585 1 89 - 96 2005年11月 [査読有り]
     
    Electrochemical communication between an electrode and a multilayered enzyme model based on "self-mediation" without a mediator was demonstrated as one of the advantages of using enzyme models instead of natural enzymes for the fabrication of biosensors. Heme undecapeptide (HP), as a peroxidase model, was immobilized on the indium-tin oxide (ITO) electrode as a multilayer and its electrocatalytic reduction for H2O2 was studied. The cathodic current of the HP multilayer-modified electrode was found to be one order of magnitude larger than that of a HP monolayer-modified electrode, in which the surface coverage of HP was one order of magnitude smaller than that of the multilayer. Since direct electron transfer between upper HP molecules and the electrode is difficult, H2O2 was reduced via an electron self-exchange between the lower HP molecules. On the basis of the self-mediation, the overall catalytic reaction rate of the HP electrode was changed by simply varying the surface coverage of HP without a mediator. Taking advantage of this system, dynamic ranges for the detection of HP inhibitors such as cyanide, imidazole and histamine were adjusted. © 2005 Elsevier B.V. All rights reserved.
  • K Komori; K Takada; T Tatsuma
    ANALYTICAL SCIENCES 21 4 351 - 353 2005年04月 [査読有り]
     
    Reduction currents for H2O2 at a heme peptide (HP)-modified electrodes are suppressed by inhibitors, such as imidazole derivatives. Although this inhibition effect allows determinations of the total inhibition ability of imidazole derivatives, it has no selectivity. In this study the selectivity control of HP-modified electrodes for imidazole derivatives was performed utilizing the thermoresponsive phase transition of poly(N-isopropylacrylamide), which was chemically immobilized on HP-modified electrodes. The inhibition ratios for imidazole derivatives appeared to be small at temperatures below the lower critical solution temperature (LCST), and to be large above the LCST. This change was ascribed to a steric hindrance caused by a phase transition of the polymer. On the other hand, the inhibition ratio for histamine, which has a larger molecular size relative to imidazole, was not significantly changed by the phase transition. Thus, the selectivity of the HP-modified electrode was found to be controllable using an immobilized phase-transition polymer.
  • K Komori; H Matsui; T Tatsuma
    BIOELECTROCHEMISTRY 65 2 129 - 134 2005年02月 [査読有り]
     
    Catalytic activity of a heme peptide (HP) modified-electrode for H2O2 reduction was controlled by use of poly(N-isopropylacrylamide) modified with an inhibitory moiety, imidazole group. The polymers inhibited the catalytic activity below their lower critical solution temperature (LCST) where the polymers were dissolved and did not inhibit the activity above the LCST where the polymers were precipitated. A polymer with a longer side chain connecting with the imidazole group was more inhibitory than a polymer with a shorter side chain at temperatures below the LCST. Formation constants of dissolved HP-imidazole complexes were evaluated by spectroscopic means, and it was found that the polymers were more inhibitory than the corresponding monomers. (c) 2004 Elsevier B.V. All rights reserved.
  • K Komori; K Yatagai; T Tatsuma
    JOURNAL OF BIOTECHNOLOGY 108 1 11 - 16 2004年02月 [査読有り]
     
    Enzymatic activity of tyrosinase was controlled on the basis of cis-trans photoisomerization of inhibitors, 4-azobenzene carboxylic acid (ACA) and 4,4'-azobenzene dicarboxylic acid (ADCA). In the case of ACA, the cis form inhibited tyrosinase-catalyzed oxidation of L-tyrosine more strongly than the trans form. On the contrary, in the case of ADCA, the cis form was less inhibitory. The oxidation rate was controlled reversibly by light irradiation in the course of the reaction. In the presence of ACA, UV light irradiation, which isomerized trans to cis form, decelerated the tyrosine oxidation, while visible light irradiation, which isomerized backward, accelerated the reaction. In contrast, in the presence of ADCA, UV light accelerated and visible light decelerated the reaction. (C) 2003 Elsevier B.V. All rights reserved.
  • T Tatsuma; K Okamura; K Komori; A Fujishima
    ANALYTICAL CHEMISTRY 74 19 5154 - 5156 2002年10月 [査読有り]
     
    Urocanic acid (UCA) is known to be synthesized as the trans isomer (trans-UCA) in the skin, and trans-UCA is transformed by UV light to the cis isomer (cis-UCA), which may be involved in photoimmunosuppression. An electrochemical method has been developed for the measurement of the concentration and isomer ratio of UCA. A heme peptide-modified electrode (HP electrode) reduces hydrogen peroxide at +150 mV vs Ag\AgCl, and the reduction current is inhibited by UCA. Since cis-UCA is a stronger inhibitor than trans-UCA, irradiation of a sample solution with UV light increases the percent inhibition. The concentration and isomer ratio of UCA in the sample solution can be estimated from the values of percent inhibition before and after the UV irradiation.
  • Electrochemiluminescence Reactions of Metal Complexes Immobilized on Surface of a Magnetic Microbead
    N. Oyama; K. Komori; O. Hatozaki
    Stud. Surf. Sci. Catal. 132 427 - 430 2001年 [査読有り]
  • T. Tatsuma; K. Komori; H.-H. Yeoh; N. Oyama
    Anal. Chim. Acta. 408 1-2 233 - 240 2000年03月 [査読有り]
     
    Colorimetric methods for the determination of cyanide and cyanogenic glycosides (amygdalin and linamarin) are developed. In the presence of dissolved oxygen, tyrosinase catalyzes the oxidation of L-tyrosine to dark melanin via red dopachrome. This coloring reaction is inhibited by cyanide, which is added or Liberated from a cyanogenic glycoside as a result of hydrolysis catalyzed by a beta-glucosidase. This inhibition is utilized to determine cyanide and the cyanogenic glycosides. A spectrophotometric method is quantitative, and its typical dynamic range is 3 mu M to 0.1 mM. Test plates that are coated by the films containing tyrosinase, a beta-glucosidase, L-tyrosine, and poly(ethyleneoxide) are also prepared as semi-quantitative devices, and their typical dynamic range is 10 mu M to 10 mM. This is expected to be an inexpensive, safe, and convenient method for end-users. (C) 2000 Elsevier Science B.V. All rights reserved.

書籍

  • In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials
    K. Komori; K. Iwasawa; R. Ogasawara; A. Suwabe; Y. Sakai (担当:分担執筆範囲:In Vitro Alveolar Epithelial Models toward the Prediction of Cytotoxicity Tests and Translocation Studies of Nanoparticles)Springer 2019年
  • 臓器チップの技術と開発動向
    小森喜久夫; 酒井康行 (担当:分担執筆範囲:（第III編・第３章）In vitro培養肺胞モデルとチップ化検討)シーエムシー出版 2018年
  • 細胞培養・品質管理の基礎知識と細胞培養機材の利用・開発・評価の留意点
    篠原満利恵; 木村圭一; 肖 文晋; ポーデル・サパナ; 小森喜久夫; 酒井康行 (担当:分担執筆範囲:第10章 細胞培養機材と細胞の関係〜第4節 ガス透過性機材と細胞の関係)（株）情報機構 2016年
  • 遺伝子医学MOOK 別冊「細胞の3次元組織化に不可欠な最先端材料技術−再生医療、その支援分野（細胞研究・創薬研究）への応用と発展のために」
    酒井康行; 篠原満利恵; 小森喜久夫; 藤井輝夫 (担当:分担執筆範囲:酸素供給に基礎を置いた3次元組織設計構築)メディカルDo 2014年
  • 現代界面コロイド化学の基礎−講義と測定マニュアル−第3版
    小森 喜久夫 (担当:分担執筆範囲:4.4.1 磁性微粒子)丸善 2009年

講演・口頭発表等

  • A-C. Eiler; P-M. Faure; J. Sugita; S. Ihida; D. Zhu; Y. Sakai; K. Fujiu; K. Komori; H. Toshiyoshi; A. Tixier-Mita
    2020 IEEE International Electron Devices Meeting (IEDM) 2020年12月 IEEE
  • Satomi Matsumoto; Eric Leclerc; Toshiro Maekawa; Haruyuki Kinoshita; Marie Shinohara; Kikuo Komori; Yasuyuki Sakai; Teruo Fujii
    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 2020年 
    Here we present a new strategy for position selective cell sampling in a microfluidic hepatocyte culture device, in which oxygen gradient is formed along a cell culture channel. Cell sampling channels where we injected PBS and trypsin were located perpendicularly to the cell culture channel. PBS sheath flows prevented trypsin diffusion and straight trypsin flows removed HepG2 cells located in different oxygen levels. Sufficient number of cells for gene expression analyses by RT-qPCR were collected by the localized trypsin flows. The presented position-selective cell sampling method would be useful to analyze hepatic metabolisms in each oxygen concentration by gene expression analysis, and leading to understanding the behavior of hepatocytes under oxygen gradient into details.
  • Satomi Matsumoto; Eric Leclec; Astia Rizki-Safitri; Mathieu Danoy; Toshiro Maekawa; Haruyuki Kinoshita; Marie Shinohara; Kikuo Komori; Yasuyuki Sakai; Teruo Fujii
    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 2019年 
    This paper reports a newly developed microfluidic hepatocyte culture device for demonstrating in vivo like drug metabolism. Medium flow during perfusion culture is used to resemble blood flow, and the gradient of oxygen concentration is formed by the cellular respiration along the cell culture channel to mimic a liver zonation. For the proof-of-concept, the metabolism of phenacetin was demonstrated in the device. We succeeded in observing phenacetin metabolism only in low oxygen area of the device, like as in vivo, by monitoring expression level of CYP3A2, CYP1A2, and reactive oxygen species (ROS).
  • 鈴木智稀; 小森喜久夫; 槌谷和義; 槌谷和義; 酒井康行; 藤井輝夫; 木村啓志; 木村啓志
    化学とマイクロ・ナノシステム学会研究会講演要旨集 2018年05月
  • Satomi Matsumoto; Eric Leclerc; Astia Rizki Safitri; Mathieu Danoy; Toshiro Maekawa; Haruyuki Kinoshita; Marie Shinohara; Kikuo Komori; Yasuyuki Sakai; Teruo Fujii
    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 2018年 
    This paper reports newly developed method for recapitulating in vivo-like hepatic function in a microfluidic hepatocyte culture device, in which oxygen gradient is formed by cellular respiration. The present microfluidic system enables us to change the expression of hepatic function by the position from the medium inlet, reducing oxygen concentration along a flow direction of culture medium. After 24h perfusion culture, we succeeded in observing in vivo-like expression of five proteins of rat primary hepatocytes in one device simultaneously. In addition, culture for longer period in the device seemed to make CYP3A2 expression stronger in only low oxygen area. These indicate that this device has a potential to be used as a pioneering method for drug screening.
  • 創薬応用を目指した肝細胞組織工学
    酒井 康行; 篠原 満利恵; 小森 喜久夫
    日本生物工学会大会講演要旨集 2017年08月 (公社)日本生物工学会
  • 鈴木 智稀; 望月 雄太; 小森 喜久夫; 槌谷 和義; 酒井 康行; 藤井 輝夫; 木村 啓志
    日本機械学会関東支部総会講演会講演論文集 2017年 一般社団法人 日本機械学会
  • 松本 倫実; Leclerc Eric; 前川 敏郎; 木下 晴之; 篠原 満利恵; 小森 喜久夫; 酒井 康行; 藤井 輝夫
    精密工学会学術講演会講演論文集 2017年 公益社団法人 精密工学会
     
    肝細胞は肝組織内の部位によって異なる機能を発現していることが知られており，本研究では肝組織内の酸素濃度勾配に着目し，これに対応した細胞機能を解析することが可能とするデバイスの開発を進めている．本報では，新たにデバイス内の異なる部位ごとに細胞を回収して解析することが可能な構造を考案し，これについて流れの解析および流量パラメータの検討などを行ったので，その結果について報告する．
  • 松本 倫実; Leclerc Eric; 前川 敏郎; 木下 晴之; 篠原 満利恵; 小森 喜久夫; 酒井 康行; 藤井 輝夫
    精密工学会学術講演会講演論文集 2016年 公益社団法人 精密工学会
     
    デバイス表面のガス透過性を低下させることで，デバイス外の環境に関わらず，上流から下流にかけての細胞による酸素消費のみによる溶存酸素濃度の変化を捉えることのできる肝細胞培養マイクロ流体デバイスを製作した．酸素感応性蛍光色素を混合した薄膜を用いてデバイス内の酸素濃度を可視化する手法を考案し，流入口と流出口に配置した酸素センサによる測定結果を用いてこの手法を評価した．
  • S. Matsumoto; E. Leclerc; T. Maekawa; H. Kinoshita; M. Shinohara; K. Komori; Y. Sakai; T. Fujii
    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 2016年 
    This paper reports a newly developed hepatocyte culture device, in which oxygen concentration gradient can be formed and monitored by fluorescence. Since the system can prevent the penetration of oxygen into the channel through gas-permeable walls of the PDMS-made device, the oxygen concentration decreases along the channel by cellular respiration. The oxygen concentration in the device is visualized with the layer containing Pt(II) Octaethylporphine (PtOEP), and evaluated by the Stern-Volmer plot. The oxygen gradient of the device was in similar range of that of human liver, and this indicates the device can make the oxygen gradient same as in vivo.
  • Efficient Formation of Size-regulated Hepatocyte Aggregates on Oxygen Permeable Microwell Sheets and the Size-dependency of Their Metabolic Capacities  [通常講演]
    M. Shinohara; K. Komori; Y. Sakai
    TISSUE ENGINEERING PART A 2015年09月 MARY ANN LIEBERT, INC
  • FUNCTIONS AND GENE EXPRESSIONS OF SANDWICH CULTURED-PRIMARY RAT HEPATOCYTES ON OXYGEN-PERMEABLE MEMBRANES UNDER PHYSIOLOGICAL OXYGEN CONCENTRATIONS  [通常講演]
    Wenjin Xiao; Hitoshi Matsui; Marie Shinohara; Kikuo Komori; Tomoharu Osada; Yasuyuki Sakai
    DRUG METABOLISM REVIEWS 2014年01月 INFORMA HEALTHCARE
  • Oxygen-permeable honeycomb microwell sheet-based formation and size-dependent functional evaluation of 3D pancreatic beta-cell and hepatocyte aggregates  [通常講演]
    M. Shinohara; H. Kimura; K. Komori; F. Fujii; Y. Sakai
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 2012年09月 WILEY-BLACKWELL
  • 酸素透過マイクロウェルを用いた肝細胞・膵β細胞凝集体の効率的再構築
    篠原満利恵; 小森喜久夫; 藤井輝夫; 酒井康行
    再生医療 2011年
  • 異所性体内埋め込み型人工肝臓開発の現状と展望  [通常講演]
    勝田 毅; 小森喜久夫; 寺谷 工; 落谷孝広; 酒井康行
    生産研究 2010年
  • KOMORI Kikuo; KAMEDA Ippei; TATSUMA Tetsu; SAKAI Yasuyuki
    Alternatives to animal testing and experimentation : AATEX 2009年12月
  • MN-O17 Development of a micropatterned cell array with an integrated optical oxygen sensor(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
    Montagne Kevin; Komori Kikuo; Tatsuma Tetsu; Fujii Teruo; Sakai Yasuyuki
    Journal of bioscience and bioengineering 2009年11月 公益社団法人日本生物工学会
  • Montagne Kevin; Komori Kikuo; Tatsuma Tetsu; Fujii Teruo; Sakai Yasuyuki
    Journal of Bioscience and Bioengineering 2009年
  • 細胞集団の迅速構築とin vitro毒性試験に有用な最小細胞集団の把握
    小森喜久夫; 亀田一平; 鈴木宏明; 篠原満利恵; 立間徹; 酒井康行
    電気化学秋季大会講演要旨集 2009年
  • Montagne Kevin; 小森 喜久夫; 立間 徹; 藤井 輝夫; 酒井 康行
    化学工学会 研究発表講演要旨集 2009年 公益社団法人 化学工学会
  • 亀田 一平; 小森 喜久夫; 立間 徹; 酒井 康行
    化学工学会 研究発表講演要旨集 2009年 公益社団法人 化学工学会
  • 名田 順; 小森 喜久夫; 立間 徹; 酒井 康行
    化学工学会 研究発表講演要旨集 2007年 公益社団法人 化学工学会
  • 小森 喜久夫; 名田 順; 立間 徹; 酒井 康行
    化学工学会 研究発表講演要旨集 2007年 公益社団法人 化学工学会
  • 中山 秀謹; 木村 啓志; 小森 喜久夫; 藤井 輝夫; 酒井 康行
    化学工学会 研究発表講演要旨集 2007年 公益社団法人 化学工学会

MISC

共同研究・競争的資金等の研究課題

  • デュアル刺激応答性電極による合律制御型電気化学センサへの応用
    JKA：
    研究期間 : 2023年04月 -2024年03月
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2021年07月 -2023年03月 
    代表者 : ティクシェ三田 アニエス; 小森 喜久夫; 酒井 康行
     
    この１年目では、予定通りこの次の結果が得られました。（１）透明TFT電極アレイ基板表面での膵β細胞培養法の構築」では、発光性ラット膵β細胞株iGLが安定して接着培養できる基板表面の条件を明確にしました。透明TFT電極アレイの電極材料は酸化インジウムスズ（ITO）であり、この表面を適切に処理することで、細胞接着因子（コラーゲンやラミニンなど）を物理的または化学的に固定化させた後、iGL細胞を播種して接着性を確認しました。また、TFT電極アレイ表面に固定化したiGL細胞に高濃度グルコースを暴露し、細胞から分泌されるインスリンをELISA技術で確認しました。「（２）膜電位の高感度評価技術の構築」では、これまでに検討してきている心筋細胞や神経細胞の透明TFT電極アレイ計測システムをベースとして、iGL細胞の膜電位計測の最適条件を明確にしました。これと並行して、心筋や神経細胞の膜電位計測で広く利用されている微小電極アレイでiGL細胞の膜電位変化応答を計測することで、TFT電極アレイでの応答と比較しました。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2018年04月 -2023年03月 
    代表者 : 木村 啓志; 小森 喜久夫
     
    創薬分野では非臨床試験において、実験動物を使ったin vivo試験や培養細胞を用いたin vitro試験が実施されているが、ヒトと実験動物の種差やin vivoとin vitroの乖離に起因する医薬品開発コストの増加が社会的問題となっている。そこで本研究では、この乖離を補完するための高次in vitro系として、マイクロ流体デバイス技術を活用した機能統合型Body-on-a-chipの構築を目指す。具体的には、本研究では医療・創薬分野への応用を見据えつつ、生物学的に新しい知見を得るための高次in vitro系として、①ADME（吸収(Absorption)・分布(Distribution)・代謝(Metabolism)・排泄(Excretion)）機能集積化、②生理学的パラメータ再現、③マイクロ電気化学センサ集積化 を実現する機能統合型Body-on-a-chipを構築する。 2021年度では、前年度までに開発したマルチウェルプレート型Organs-on-a-chipを用いて、初代培養肝細胞および標的がん細胞の共培養系を確立し、薬効毒性試験を実施した。その機能検討を実施した。この結果、創薬プロセスで従来利用されているコンディションド培地を利用した薬効結果と比して、我々のシステムをOrgans-on-a-chipを利用した結果では、薬効結果に差違が生じることが明らかとなった。機能統合に向けた各要素の改良検討については、スターラ式ポンプの駆動条件を明らかにすると共に、電気化学式グルコースセンサについては、研究分担者の近畿大学小森と共同して改良とその評価を実施し、それぞれの機能の集積化を実現したマルチウェルプレート型のOrgans-on-a-chipの完成に至った。
  • 日本学術振興会：科学研究費助成事業 基盤研究(C)
    研究期間 : 2019年04月 -2022年03月 
    代表者 : 小森 喜久夫
     
    ヒト応答予測の向上を目指し、酸素非依存性の電気化学バイオセンサでのモニタリングを利用することで、細胞応答を非侵襲で評価するためのシステム開発を検討した。肝細胞については、電気化学グルコースセンサに好気的培養手法を組み合わせることで、生体内の門脈近傍の肝組織と同質の糖代謝・糖新生の現象を電流応答で記述できた。この他にも、アレルギー研究で広く利用される好塩基球様細胞株RBL-2H3が免疫刺激によって遊離するヒスタミンの電気化学モニタリングにも成功している。この手法は、細胞アッセイの予測精度向上に繋がるものと期待される。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2014年04月 -2017年03月 
    代表者 : 小森 喜久夫
     
    本研究では、グラフェン表面からカーボンナノチューブを成長させた３次元ハイブリッド膜（CNTs/G膜）を用いて、酵素西洋ワサビペルオキシダーゼなどのバイオキャタリストの固定化量を増大させることにより、グラフェンを用いるよりも、高感度な電気化学バイオセンサの構築を試みた。グラフェンと比べて、CNTs/G膜ではバイオキャタリストの固定化量を実際に増大させることが可能であり、基質の過酸化水素に対する触媒還元電流も向上した。したがって、CNTs/G膜を用いることで、グラフェンよりも高感度な電気化学バイオセンサを構築でき、この他にも、酵素を用いたバイオ燃料電池の電極にも利用できるものと期待される。
  • 日本学術振興会：科学研究費助成事業
    研究期間 : 2009年 -2011年 
    代表者 : 小森 喜久夫
     
    本研究では、三次元細胞凝集体の大きさを制御し、生体内での主要な毒性発現メカニズムと同質の応答を示す凝集体の最小サイズを明らかにすることを目的とした。マイクロウェル構造を配備したポリジメチルシロキサン(PDMS)テンプレートを作製し、マイクロウェル内でヒト肝ガン細胞株Hep G2の凝集体を形成させた。マイクロウェルの大きさを調節することにより、細胞凝集体の大きさを制御した。薬物代謝酵素シトクロムP450 1A1/2(CYP 1A1/2)の活性を調べたところ、直径200μm・高さ約60μm以上の大きさの凝集体の活性は、直径63μm・高さ約70μmの凝集体のものよりも3. 5倍高くなった。また、CYP 1A1/2に代謝されてより強い毒性を示すアフラトキシンB1(AFB1)を凝集体に暴露したところ、直径200μm以上での凝集体の用量作用曲線は、直径63μmのものよりも1桁低濃度側にシフトした。また、ラット成熟初代肝細胞でも同様の検討を行ったところ、直径110μmの球状の凝集体のAFB1に対する用量作用曲線は、直径35μmの球状のものよりも1桁低濃度側にシフトした。一方、酸素感受性の発光色素白金オクタエチルポルフィリン(PtOEP)をマイクロウェルの底面にコートした、凝集体の呼吸活性を非侵襲的に評価可能なガラス製プレートの開発も試みた。マイクロウェル内で肝ガン細胞株Hep G2の三次元細胞組織を培養したところ、PtOEPの発光強度から、細胞組織の呼吸活性能を評価できた。これらのことから、本方法論を用いることにより、生理学的に同質の応答を示す各種臓器由来の細胞凝集体の大きさを決定できるものと期待される。

委員歴

  • 2022年 - 現在   電気化学会   分子機能電極研究会 幹事
  • 2022年 - 現在   電気化学会   代議員
  • 2022年01月 - 現在   日本動物実験代替法学会   WEB企画検討ワーキンググループ
  • 2021年 - 現在   日本動物実験代替法学会   評議員
  • 2019年 - 現在   Sens. Actuators Report   Editorial Board Members
  • 2018年 - 2019年   電気化学会   代議員
  • 2017年01月 - 2018年12月   日本動物実験代替法学会   総務委員
  • 2017年 - 2018年   電気化学会   普及委員
  • 2016年 - 2017年   電気化学会   編集委員
  • 2013年 - 2016年   電気化学会   関東支部幹事
  • 2008年01月 - 2012年12月   日本動物実験代替法学会   総務委員
  • 2007年01月 - 2008年12月   日本動物実験代替法学会   広報委員
  • 2006年   日本動物実験代替法学会   第20回大会 事務局長

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