TANAKA Kenji

Researcher Information

Degree

• Ph.D
• (BLANK)

URL

http://www.fuk.kindai.ac.jp/07kaku-gakka/frame-chemistry.htm

J-Global ID

• 200901066041593902

Research Interests

• 海洋分解性プラスチック   PHBH   Cupriavidus necator   Ideonella   carbon dioxide fixation   lactic acid bacteria   high cell density cultivation   Lactate fermentation   rotifer   ＰＨＢ   Ralstonia eutropha   green plastic   hydrogen oxidizing bacteria   Biochemical Engineering   Applied microbiology・Applied biochemistry   

Research Areas

• Life sciences / Food sciences
• Life sciences / Applied biochemistry
• Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering

Academic & Professional Experience

• 2022/09  近畿大学産業理工学部学部長補佐
• 2007/04 - 2008/03  Kinki Univ., Fac. of Humanity-Oriented Science and Engineering生物環境化学科准教授
• 2004/04 - 2007/03  Kinki Univ., Fac. of Humanity-Oriented Science and Engineering生物環境化学科助教授
• 2000/04 - 2002/03  Kinki Univ., Fac. of Kyushu Engineering生物環境化学科講師
• 1991 - 1999  Research associate,
• Faculty of Agriculture, Kyushu University大学院農学研究院
• Dept of Food Science & Technology,

Education

• 1989/04 - 1991/03  九州大学大学院  農学研究科  食糧化学工学専攻博士後期課程
•        - 1991  Kyushu University  Graduate School, Division of Agriculture
• 1983/04 - 1987/03  Kyushu University  School of Agriculture  食糧化学工学科
•        - 1987  Kyushu University  Faculty of Agriculture
• 1979/04 - 1982/03  福岡県立嘉穂高等学校
•        -   九州大学大学院  農学研究科  食糧化学工学博士専攻前期課程

Association Memberships

• JAPANESE SOCIETY FOR FOOD HYGIENE AND SAFETY   JAPANESE SOCIETY OF MICROBICAL ECOLOGY   JAPANESE SOCIETY FOR BACTERIOLOGY   日本乳酸菌学会   日本水産学会   日本生物工学会   日本農芸化学会   

Published Papers

• Novel multicellular prokaryote discovered next to an underground stream

  Kouhei Mizuno; Mais Maree; Toshihiko Nagamura; Akihiro Koga; Satoru Hirayama; Soichi Furukawa; Kenji Tanaka; Kazuya Morikawa

  eLife eLife Sciences Publications, Ltd 11 2022/10 [Refereed]
   

  A diversity of prokaryotes currently exhibit multicellularity with different generation mechanisms in a variety of contexts of ecology on Earth. In the present study, we report a new type of multicellular bacterium, HS-3, isolated from an underground stream. HS-3 self-organizes its filamentous cells into a layer-structured colony with the properties of a nematic liquid crystal. After maturation, the colony starts to form a semi-closed sphere accommodating clusters of coccobacillus daughter cells and selectively releases them upon contact with water. This is the first report that shows that a liquid-crystal status of cells can support the prokaryotic multicellular behavior. Importantly, the observed behavior of HS-3 suggests that the recurrent intermittent exposure of colonies to water flow in the cave might have been the ecological context that cultivated the evolutionary transition from unicellular to multicellular life. This is the new extant model that underpins theories regarding a role of ecological context in the emergence of multicellularity.
• Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 by a Recombinant Cupriavidusnecator

  Kenji Tanaka; Kazumasa Yoshida; Izumi Orita; Toshiaki Fukui

  Bioengineering MDPI AG 8 (11) 179 - 179 2021/11 [Refereed][Invited]
   

  The copolyester of 3-hydroxybutyrate (3HB) and 3-hydoxyhexanoate (3HHx), PHBHHx, is one of the most practical kind of bacterial polyhydroxyalkanoates due to its high flexibility and marine biodegradability. PHBHHx is usually produced from vegetable oils or fatty acids through β-oxidation, whereas biosynthesis from sugars has been achieved by recombinant strains of hydrogen-oxidizing bacterium Cupriavidus necator. This study investigated the biosynthesis of PHBHHx from CO2 as the sole carbon source by engineered C. necator strains. The recombinant strains capable of synthesizing PHBHHx from fructose were cultivated in a flask using complete mineral medium and a substrate gas mixture (H2/O2/CO2 = 8:1:1). The results of GC and 1H NMR analyses indicated that the recombinants of C. necator synthesized PHBHHx from CO2 with high cellular content. When 1.0 g/L (NH4)2SO4 was used as a nitrogen source, the 3HHx composition of PHBHHx in the strain MF01∆B1/pBBP-ccrMeJ4a-emd was 47.7 ± 6.2 mol%. Further investigation demonstrated that the PHA composition can be regulated by using (R)-enoyl-CoA hydratase (PhaJ) with different substrate specificity. The composition of 3HHx in PHBHHx was controlled to about 11 mol%, suitable for practical applications, and high cellular content was kept in the strains transformed with pBPP-ccrMeJAc-emd harboring short-chain-length-specific PhaJ.
• 微生物機能を利用したバイオプラスチックの生産

  福居俊昭; 田中賢二

  科学と工業 95 (5) 1 - 7 2021/05 [Invited]
  
• Metabolic Engineering for Biosynthesis of P(3HB-co-3HHx) from Sugars, CO2, and Methanol.

  Toshiaki Fukui; Izumi Orita; Kenji Tanaka

  Proceedings of The 7th International Conference on Bio-Based Polymers for Bio-Circular-Green Economy（ICBP2019) 2019/11
• Microbial production of polyhydroxyalkanoates from CO2 by hydrogen-oxidizing bacteria

  Tanaka K; Suzuki N; Izumi O; Fukui T

  Proceedings of International Symposium on Biopolymer Synthesis and Degradation 14 - 15 2019/07 [Invited]
  
• Enhancement of lactate fraction in poly(lactate-co-3-hydroxybutyrate) synthesized by Escherichia coli haboring the D-lactate dehydrogenase gene from Lactobacillus acetotolerans HT

  Saki Goto; Naoyuki Suzuki; Kenichiro Matsumoto; Seiichi Taguchi; Kenji Tanaka; Hiromi Matsusaki

  The Journal of General and Applied Microbiology Microbiology Research Foundation in press (4) 204 - 208 0022-1260 2019/05 [Refereed]
  
• Biosynthesis of novel lactate-based polymers containing medium-chain-length 3-hydroxyalkanoates by recombinant Escherichia coli strains from glucose

  Hiromi Matsusaki; Ayaka Hokamura; Hideki Shiratsuchi; Hideki Abe; Ken'ichiro MATSUMOTO; Kenji Tanaka; Saki Goto; Seiichi Taguchi

  Journal of Bioscience and Bioengineering in press (2) 191 - 197 2019 [Refereed]
   

  Novel lactate (LA)-based polymers containing medium-chain-length 3-hydroxyalkanoates (MCL-3HA) were produced in fadR-deficient Escherichia coli strains from glucose as the sole carbon source. The genes encoding LA and 3-hydroxybutyrate (3HB) monomers supplying enzymes [propionyl-CoA transferase (PCT), d-lactate dehydrogenase (D-LDH), β-ketothiolase (PhaA), and NADPH-dependent acetoacetyl-CoA reductase (PhaB)], MCL-3HA monomers supplying enzymes [(R)-3-hydroxyacyl-ACP thioesterase (PhaG) and (R)-3-hydroxyacyl (3HA)-CoA ligase] via fatty acid biosynthesis pathway, and modified polyhydroxyalkanoate (PHA) synthase [PhaC1(STQK)] of Pseudomonas sp. 61-3 were introduced into E. coli LS5218. This resulted in the synthesis of a novel LA-based copolymer, P(LA-co-3HB-co-3HA). 1H-nuclear magnetic resonance (NMR) analysis revealed the composition of P(LA-co-3HB-co-3HA) to be 19.7 mol% LA (C3), 74.9 mol% 3HB (C4), and 5.4 mol% MCL-3HA units of C8 and C10. Furthermore, the recombinant E. coli CAG18497 strain carrying these genes, excluding the phaAB genes, accumulated P(92.0% LA-co-3HA) with a novel monomer composition containing C3, C8, C10, and C12. 13C-NMR analysis showed the existence of LA-3HA sequence in the polymer. The solvent cast film of P(92.0% LA-co-3HA) exhibited transparency similar to poly(lactic acid).
• Cloning and heterologous expression of lactate dehydrogenase genes from acid-tolerant Lactobacillus acetotolerans HT

  Saki Goto; Akane Motomura; Ai Kawahara; Hideki Shiratsuchi; Kenji Tanaka; Hiromi Matsusaki

  Food Sci. Technol. Res. Japanese Society for Food Science and Technology 24 (5) 861 - 868 1344-6606 2018/09 [Refereed]
  
• Improvement of monomer composition in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesized from CO2 by genetically engineered hydrogen-oxidizing bacterium

  Tanaka K; Suzuki N; Mori S; Izumi O; Fukui T

  Proceedings of World Congress on Biopolymers and Bioplastics (Biopolymer 2018) 71  2018/08 [Refereed]
  
• A Novel Efficient Antibacterial Action of ABC semiconductors

  Kengo Ito; Tohru Nishibe; Masakazu Kawahara; Seiichiro Tsujitsuka; Kenji Tanaka

  Proceedings of 10th International Symposium on Organic Molecular Electronics (ISOME 2018) 2018/03 [Refereed]
  
• Antimicrobial effect of a novel photosemiconductor composed of Ag nanoplate, boron and clay, and its increase by visible light irradiation

  Kenji Tanaka; Kojiro Yamamoto; Seiichiro Tsujituka; Kengo Itou

  Proceedings of BIT’s 4th Annual World Congress of Smart Materials-2018 (WCSM-2018) 422 - 422 2018/03 [Refereed]
  
• Effect of adding fatty acid in culture medium on cell growth of acid tolerant lactic acid bacterium

  Kenji Tanaka; Kiko Sakai; Saki Goto; Hiromi Matsusaki

  International Journal of Science and Engineering Investigations 6 (69) 78 - 81 2017/10 [Refereed]
  
• BIOSYNTHESIS OF POLY (3-HYDROXYBUTYRATE -CO- 3-HYDROXYHEXANOATE) FROM FRUCTOSE AND CO2 BY THE ENGINEERED RALSTONIA EUTROPHA

  Kenji Tanaka; Kiko Sakai; Ryo Sawada; Saki Goto; Hiromi Matsusaki

  Proceedings of Biomaterials International 2017, Fukuoka Japan 1 - 4 2017/08 [Refereed]
  
• Effect of fatty acid in culture medium on growth of acid tolerant lactic acid bacterium, Lactobacillus acetotolerans strain HT

  TANAKA Kenji

  Proceedings of The 6th International Congress o Natural Sciences and Engineering (2017 ICNSE) 129 - 135 2017/07 [Refereed]
  
• Growth characteristics of newly isolated xylose-assimilating bacterium and accumulation of green plastic, polyhydroxyalkanoate in the genetic engineered strain

  Kenji Tanaka; Shunya Mori; Kastuki Matsumoto; Kojiro Yamamoto; Izumi Wakida; Saki Goto; Hiromi Mastusaki

  International Journal of Biological Engineering 6 (1) 1 - 6 2016/10 [Refereed]
  
• Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C6-monomer composition from CO2 by recombinant of Ralstonia eutropha. Journal of Bioremediation & Biodegradation

  Kenji Tanaka; Shunya Mori; Kouki Maruo; Orita Izumi; Toshiaki Fukui

  Journal of Bioremediation & Biodegradation 7 (5(suppl)) 64  2016/09 [Refereed]
  
• Growth characteristics of newly isolated xylose-assimilating bacterium and accumulation of green plastic, polyhydroxyalkanoate in the genetic engineered strain

  Kenji Tanaka; Shunya Mori; Kastuki Matsumoto; Kojiro Yamamoto; Izumi Wakida; Saki Goto; Hiromi Mastusaki

  Proceedings of the International Symposium on Life Science & Biological Engineering 2016 (I SLSBE2016), August 25-27, Tokyo 628 - 638 2016/08 [Refereed]
  
• Preparation of genetic engineered Enterobacter strain TF to produce green plastic from xylose

  Shunya Mori; Kojiro Yamamoto; Kastuki Matsumoto; Ayaka Hokamura; Hiromi Mastusaki; Kenji Tanaka

  REPORTS of Faculty of Humanity-Oriented Science and Engineering KINDAY UNIVERSITY 近畿大学産業理工学部 24 (24) 6 - 10 1349-5801 2016/06 

  Polyhydroxyancanoate (PHA) is a bacterial polyester which is thermoplastic, flexible and biodegradable. PHA is expected as "green plastic" material therefore many researchers have been studying the development of new type PHAs, their physical property, biodegradability and the production method. In commercial production of PHA, use of substances obtained from biomass as economic and ecological carbon sources is necessary. Hemicellulose, which is mainly composed of glucose, xylose and other monosaccharaides is an important biomass, however xylose is not very feasible sugar to be used as substrate in fermentation. Our isolated bacterium, Enterobacter sp.TF grow vigorously on xylose, arabinose and other many kind of sugars but it does not accumulate PHA. Hence, we made genetic engineering to the strain TF by inserting the genes for PHA biosynthesis from Ralstonia eutropha which is well known as PHB producer, and from Pseudomonas sp.61-3 which is one of the few bacteria to produce PHA copolymer from glucose. As, result, our recombinant of Enterobacter sp.TF did not produce PHA copolymer but produced about 20wt% PHB homopolymer per dried cells.キシロースは、農産廃棄物や廃材など植物性バイオマスに含まれるヘミセルロースの主要構成単糖であり、発酵原料等への有効利用法の開発が急がれている。しかしながら、キシロースは微生物にとって利用しやすい糖であるとは言い難く、また工業的に利用価値のある発酵生産物も少ないのが現状である。ある種の微生物が産生するポリヒドロキシ酪酸（PHB）やポリヒドロキシアルカン酸（PHA）などのポリエステルは、熱可塑性と生分解性を併せ持つため"グリーンプラスチック"として期待されており、植物由来の有機物を原料基質として使えばCO2の排出量を増やさない"カーボンニュートラル"なプラスチックにもなりえる。本研究では、独自に分離したキシロース高資化性のEnterobacter属細菌にPHAやPHBの生合成遺伝子を導入した組換え株を作製し、実際にキシロースからこれらグリーンプラスチックを合成するか検証した。
• Autotrophic Growth of Paracoccus denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO2

  Kenji Tanaka; Syunya Mori; May Hirata; Hiromi Matsusaki

  Environment and Ecology Research 4 (4) 231 - 236 2016 [Refereed]
  
• Inhibitory effect to pathogenic bacteria of visible light responsive type nanosilver photocatalyst

  Shintaro Tajiri; Megumi Miyazaki; Kenji Tanaka; Kengo Ito

  2015 International Chemical Congress of Pacific Basin Societies, Section ORG 937 2015/12
• Acid-tolerant lactic acid bacterium isolated from rice vinegar

  Kenji Tanaka; Shintaro Tajiri; Ryo Sawada; Yoshihiro Kawamoto; Teruaki Matsubara; Munehiro Hosihino; Hiromi Matsusaki

  International Journal of Research in Applied,Natural and Social Sciences 3 (10) 29 - 36 2015/10 [Refereed]
  
• Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose

  Ayaka Hokamura; Izumi Wakida; Yuki Miyahara; Takeharu Tsuge; Hideki Shiratsuchi; Kenji Tanaka; Hiromi Matsusaki

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 120 (3) 305 - 310 1389-1723 2015/09 [Refereed]
   

  The polyhydroxyalkanoate (PHA) copolymers consisting of short-chain-length (Scl) and medium-chain-length (mcl) monomers have various properties ranging from stiff to flexible depending on the molar fraction of the monomer compositions. It has been reported that PhaG, which is first known as a (R)-3-hydroxyacyl-acyl carrier protein (ACP)-CoA transferase, actually functions as a 3-hydroxyacyl-ACP thioesterase, and the product of PP0763 gene from Pseudomonas putida KT2440 has a (R)-3-hydroxyacyl (3HA)-CoA ligase activity (Wang et al., Appl. Environ. Microbiol., 78, 519-527, 2012). In this study, we found a new (R)-3HA-CoA ligase (the product of PA3924 gene) from Pseudomonas aeruginosa PAO. The PA3924 gene was coexpressed with PHA synthase 1 gene (phaC1(Ps)) and phaG(Ps) gene from Pseudomonas sp. 61-3, and beta-ketothiolase gene (phbA(Re)) and acetoacetyl-CoA reductase gene (phbB(Re)) from Ralstonia eutropha in Escherichia coli LS5218 at 25 degrees C. As a result, the copolymer containing 94.6 mol% 3-hydroxybutyrate (3HB) and 5.4 mol% mcl-3-hydroxyalkanoates (3HA) consisting of C-8, C-10, C-12 and C-14 was synthesized by recombinant E. coli LS5218 from glucose as the sole carbon source. The concentration of P(3HB-co-3HA) (scl-co-mcl-PHA) synthesized by the recombinant E. coli LS5218 harboring phaC1(Ps), phaG(Ps), phbAB(Re) and the PA3924 genes was approximately 7-fold higher than that of the recombinant LS5218 harboring phaC1(Ps), phaG(Ps), phbAB(Re) and the PP0763 genes. The number-average molecular weight of the P(3HB-co-5.4% 3HA) copolymer was 233 x 10(3), which was relatively high molecular weight. In addition, the physical and the mechanical properties of the copolymer were demonstrated to improve the brittleness of P(3HB) homopolymer. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.
• Acid-tolerant lactic acid bacterium isolated from rice vinegar

  Kenji Tanaka; Shintaro Tajiri; Ryo Sawada; Yoshihiro Kawamoto; Teruaki Matsubara; Munehiro Hosihino; Hiromi Matsusaki

  Proceeding of The Annual Conference on Life Sciences and Engineering 2015(2015 ACLSE) Part 3 118 - 125 2015/08 [Refereed]
  
• The Effect of Visible Light with Different Wavelength and Intensities on Population Growth of Marine Zooplankton, Brachionus Plicatilis

  Kenji Tanaka; Tomohito Amai; Tomohiro Kuratomi; Katsuki Matsumoto; Shintaro Tajiri

  Proceedings of The 2nd International Biotechnology, Chemical Engineering and Life Science Conference (IBCELC 2015) Part 3 98 - 104 2015/07 [Refereed]
  
• Isolation of extremely acid-resistant acetic acid bacterium and its application to production of high-acid vinegar in mesophilic condition

  Shintaro Tajiri; Katsuki Matsumoto; Tomohiro Kuratomi; Kenji Tanaka; Hideo Iwai; Hiro Ueno; Munehiro Hoshino

  REPORTS of Faculty of Humanity-Oriented Science and Engineering KINKI UNIVERSITY 近畿大学産業理工学部 22 (22) 1 - 6 1349-5801 2015/06 

  [概要]福岡県内のお酢メーカーで受け継がれてきた高酸度酢醸造用種酢から酸に対して高度に耐性な酢酸菌を分離した. 16SrRNA遺伝子の相同性その他の形質より本菌をグルコンアセトバクター属細菌と推定し, Gluconacetobacter sp. Munehiroと命名した. 高酸度酢の生産効率化を目的として, 発酵中に基質エタノールを連続的に供給補充する流加培養法において, 発酵槽内のエタノール濃度が酢酸生産速度と酢酸生産量に及ぼす影響を調べた. その結果をもとに, 発酵槽内のエタノール濃度が40 g·L-1から20 g·L-1, 10 g·L-1へと段階的に下がるようエタノール供給を調整することによって, 酢酸の阻害作用を受けやすい中温域（29℃）で発酵槽を冷却することなく205 g·L-1という従来に無い高酢酸濃度のお酢生産に成功した. [Abstract]A new acid-resistant acetic acid bacterium was isolated from the seed vinegar which has been used for the production of highacid vinegar. The bacterium was gram-negative, rod-shaped and motile ; it occurred singly and did not over-oxidize acetic acid. Cellulose formation was not detected. We named the bacterium Gluconacetobacter sp. Munehiro because the sequence of the 16S rRNA gene had 99.9% similarity to that of G. europaeus. The strain is extremely resistant to acetic acid and produces high-concentration acetic acid under mesophilic culture condition. The effects of maintaining the ethanol concentration at different levels on the production of high-acid vinegar were investigated. The acetic acid concentration reached 205 g·L-1 by the stepwise lowering of the ethanol concentration from 40 g·L-1, 20 g·L-1 to 10 g·L-1 at 29℃ without cooling the fermenter. However, the cell concentration, the specific growth rate and the acetic acid production rate were very low because the cell culture suffered by long-time oxygen limitation.
• 第2章 LED等を用いた光照射技術の活用による動物性餌料生物培養の省エネルギー生産技術の開発. 有用水産生物の光応答メカニズムの解明及び高度利用技術の開発.

  田中 賢二

  農林水産省研究成果 536 44 - 49 2015/02
• YB1 binds to and represses the p16 tumor suppressor gene

  Yojiro Kotake; Yuichi Ozawa; Masanori Harada; Kyoko Kitagawa; Hiroyuki Niida; Yasutaka Morita; Kenji Tanaka; Takafumi Suda; Masatoshi Kitagawa

  GENES TO CELLS WILEY-BLACKWELL 18 (11) 999 - 1006 1356-9597 2013/11 [Refereed]
   

  Y box binding protein 1 (YB1) has multiple functions associated with drug resistance, cell proliferation and metastasis through transcriptional and translational regulation. Increased expression of YB1 is closely related to tumor growth and aggressiveness. We showed that YB1 protein levels were decreased through replicative and premature senescence and were correlated with increased expression levels of p16(INK4A) tumor suppressor gene. Depletion of YB1 was associated with increased levels of p16 in human and murine primary cells. Forced expression of YB1 in mouse embryonic fibroblasts resulted in decreased expression of p16 and increased cell proliferation. Senescence-associated expression of -galactosidase was repressed in YB1-over-expressing cells. Chromatin immunoprecipitation assays showed that YB1 directly associates with the p16 promoter. Taken together, all our findings indicate that YB1 directly binds to and represses p16 transcription, subsequently resulting in the promotion of cell growth and prevention of cellular senescence.
• Increase of Nitrite in Tarako and Mentaiko during Manufacture and C

  TANAKA Kenji

  NIPPON SHOKUHIN KOGYO GAKKAISHI Japanese Society for Food Science and Technology 60 (6) 270 - 277 1341-027X 2013/06 [Refereed]
   

  Increases in nitrite ions in tarako, ikura and sujiko during manufacture or cold storage have been observed. We isolated 30 bacterial strains from tarako and examined their nitrite-producing abilities. Eleven strains among the isolates reduced nitrate to nitrite at 0-5 % NaCl and 5-30°C in a succinate-nitrate liquid medium. 16S rRNA gene sequence analysis indicated that these nitrate-reducing bacteria belonged to Vibrio, Halomonas or Cobetia. Two strains (strains No. 6 and No. 19) that showed the highest nitrite production among the isolates, and one strain (strain No. 17) that showed the lowest nitrite production, were inoculated in tarako and mentaiko. Then, they were tested for nitrite production after storage at 5, 10 and 20°C. Results showed that strain No. 19 produced nitrite ions at every tested temperature. We also found that frozen Alaska pollack roe imported from the USA and Russia were contaminated with nitrite-producing bacteria from 8 to 340 CFU/g. These results suggest that the increase in nitrite ions in tarako and mentaiko is due to the reduction of ingredient-derived nitrate to nitrite by nitrate-reducing bacteria that contaminate Alaska pollack roe.
• Microbial production of poly(hydroxybutyrate) from C1 carbon sources

  Kianoush Khosravi-Darani; Zahra-Beigom Mokhtari; Tomohito Amai; Kenji Tanaka

  Applied Microbiology and Biotechnology 97 (4) 1407 - 1424 0175-7598 2013/02 [Refereed]
   

  Polyhydroxybutyrate (PHB) is an attractive substitute for petrochemical plastic due to its similar properties, biocompatibility, and biodegradability. The cost of scaled-up PHB production inhibits its widespread usage. Intensive researches are growing to reduce costs and improve thermomechanical, physical, and processing properties of this green biopolymer. Among cheap substrates which are used for reducing total cost of PHB production, some C1 carbon sources, e.g. methane, methanol, and CO2 have received a great deal of attention due to their serious role in greenhouse problem. This article reviews the fundamentals of strategies for reducing PHA production and moves on to the applications of several cheap substrates with a special emphasis on methane, methanol, and CO2. Also, some explanation for involved microorganisms including the hydrogen-oxidizing bacteria and methanotrophs, their history, culture condition, and nutritional requirements are given. After description of some important strains among the hydrogen-oxidizing and methanotrophic producers of PHB, the article is focused on limitations, threats, and opportunities for application and their future trends. © 2013 Springer-Verlag Berlin Heidelberg.
• 光照射がワムシ増殖とビタミンB12生成に及ぼす効果

  田中 賢二

  アクアネット 湊文社 16 (7) 31 - 35 2013 [Refereed]
  
• III-3. Relation between growth of rotifer and nutrition condition under photoirradiation

  Kenji Tanaka

  Nippon Suisan Gakkaishi (Japanese Edition) 79 (5) 888  0021-5392 2013 [Refereed]
  
• Genus-specific and phase-dependent effects of nitrate on a sulfate-reducing bacterial community as revealed by dsrB-based DGGE analyses of wastewater reactors

  Kouhei Mizuno; Yui Morishita; Akiko Ando; Naofumi Tsuchiya; Mai Hirata; Kenji Tanaka

  WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY SPRINGER 28 (2) 677 - 686 0959-3993 2012/02 [Refereed]
   

  The biogenic production of hydrogen sulfide is a serious problem associated with wastewater treatment. The aim of this study was to investigate the inhibitory effect of nitrate on the dynamics of sulfate-reducing bacteria (SRB) community in a laboratory-scale wastewater reactor, originating from a denitrifying plant using activated sludge. For this purpose, denaturing gradient gel electrophoresis (DGGE) analysis targeting the dsrB (dissimilatory sulfite reductase) gene was used in combination with chemical analyses and measurement of oxidation and reduction potential (ORP). The reactors were initially dosed with 1.0 and 4.0 g/L potassium nitrate and anaerobically incubated for 490 h. Addition of 4.0 g/L nitrate to the reactor was associated with a prolonged inhibition (over 300 h, i.e., 12.5 days) of sulfate reduction and this was consistent with a rapid decrease in ORP associated with nitrate depletion. The DGGE analysis revealed that nitrate addition remarkably attenuated a distinct group of dsrB related to Desulfovibrio, whereas other dsrB groups were not influenced. Furthermore, another sulfate reduction by Syntrophobacter in the later stages of the incubation period occurred in both reactors (regardless of the nitrate concentration), suggesting that different SRB groups are associated with sulfate reduction at different stages of the wastewater treatment process.
• Culture method of chemolithoautotrophic bacteria assimilating hydrogen and carbondioxide

  Kenji Tanaka; Tomohito Amai

  Reports of School of Humanity-Oriented Science and Engineering, Kinki University 近畿大学産業理工学部 16 (16) 1 - 7 1349-5801 2012 

  [Abstract] Chemolithoautotrophic bacteria are the bacteria that grow without utilizing organic matters as substrate and photosynthesis. They acquire energy for cell growth by oxidizing inorganic compounds and assimilate CO_2 to synthesis cell components and other organic compounds. They have important role of CO2 fixation and primary producer, matter cycle environmental clean-up in nature, and are used for waste treatment. Manufacture of useful substances from CO_2 using chemolithoautotriphic bacteria is also expected. Among the chemolitoautotrophic bacteria, this article focuses on usefulness, handling and basal culture method of hydrogen-oxidizing bacteria under autotrophic condition.
• Genus-specific effects of nitrate on a sulfate-reducing bacterial community

  Yui MORISHITA; Souya HARADA; Kouhei MIZUNO; Akiko ANDO; Mai HIRATA; Kenji TANAKA

  Research Report of Kitakyushu National College of Technology Kitakyushu National College of Technology 45 (44) 69 - 72 2185-7598 2012
• Cell growth and P(3HB) accumulation from CO2 of a carbon monoxide-tolerant hydrogen-oxidizing bacterium, Ideonella sp O-1

  Kenji Tanaka; Kenta Miyawaki; Akane Yamaguchi; Kianoush Khosravi-Darani; Hiromi Matsusaki

  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER 92 (6) 1161 - 1169 0175-7598 2011/12 [Refereed]
   

  Cell growth and accumulation of polyhydroxybutyric acid, P(3HB), from CO2 in autotrophic condition of a newly isolated hydrogen-oxidizing bacterium, the strain O-1, was investigated. The bacterium, which was deposited in the Japan Collection of Microorganisms as JCM17105, autotrophically grows by assimilating H-2, O-2, and CO2 as substrate. 16S rRNA gene sequence of the bacterium was the closest to Ideonella dechloratans (99%). Specific growth rate of the strain O-1 was faster than a hydrogen-oxidizing bacterium, Ralstonia eutropha, which is well-known P(3HB)-producing microorganism. The strain O-1 is tolerant to high O-2 concentration and it can grow above 30% (v/v) O-2, while the growth of R. eutropha and Alcaligenes latus was seriously inhibited. In culture medium containing 1 g/L (NH4)(2)SO4, cell concentration of the strain O-1 and P(3HB) increased to 6.75 and 5.26 g/L, respectively. The content of P(3HB) in the cells was 77.9% (w/w). The strain O-1 was very tolerant to carbon monoxide (CO) and it grew even at 70% (v/v) CO, while the growth of R. eutropha and A. latus were seriously inhibited at 5% (v/v) CO. From these results, it is expected that the strain O-1 will be useful in the manufacture of P(3HB) because the industrial exhaust gas containing CO2, H-2, and CO can be directly used as the substrate in the fermentation process.
• 資源管理型漁業のための微生物餌料とその利用技術の開発

  田中 賢二

  平成22年度科学研究費補助金 基盤研究(C)研究成果報告書 2011
• Diversity Analysis of Sulfate-Reducing Bacterial Communities in a Wastewater Treatment Plant Using Dissimilatory Sulfite Reductase (dsrB) Gene as a Molecular Marker

  Takanori Sada; Kouhei Mizuno; Yui Morishita; Akiko Andou; Naofumi Tsuchiya; Takahiro Aoyagi; Kenji Tanaka

  Proceedings of The second Japan-Korea joint symposium on bio-microsensing technology (2nd JKBT) Increasing Importance of Bio-microsensing Technology in Medical and Dental Areas Kitakyushu, Japan 27 - 28 2009/11 [Invited]
  
• 発電所排水の窒素処理工程の停止条件に関する検討

  安東晶子; 土谷直史; 田中賢二; 水野康平; 潮田直城

  平成20年度九州電力株式会社総合研究所研究報告 (No.09045) 1 - 74 2009
• Preliminary trials on the effect of lighting for the population growth of the rotifer, Brachionus plicatilis

  Takao Yoshtmatsu; Takahiro Higuchi; Yuji Hamasaki; Kenji Tanaka

  JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES 42 (2) 131 - 136 0021-3551 2008/04 

  The effect of lighting to rotifer culture water was investigated preliminarily using freshwater Chlorella as food in terms of vitamin B-12 (VB12) production. The marine rotifer Brachionus plicatilis was batch-cultured for five days at 25 degrees C in 25 psu seawater with or without lighting (L:D = 13: 11 and L:D = 0:24). Rotifers were fed VB12-enriched or VB12-free Chlorella vidgaris with or without cobalt compound supplementation (cobalt (11) sulfate heptahydrate: CoSO4 center dot 7H(2)O). When VB12-enriched Chlorella was fed, rotifer with lighting showed better population growth than in complete dark. On the other hand, no difference was observed in the population growth between light and dark groups when VB12-free Chlorella was used. Nevertheless if the cobalt compound was supplemented to VB12-free Chlorella, the light group showed higher population growth than non-supplemented groups and dark groups, and a much higher amount of VB12 was detected from the tanks than those from other groups. In addition to that, even in the group fed VB12-free Chlorella without Co supplementation a daily increase of a small amount of VB12 was observed when they were lit up. From these experimental results, we can conclude that lighting plays an important role for the population growth of rotifers and Co compound supplementation to rotifer culture water promotes the reproduction performance of rotifers due to the enhanced production of VB12 by lighting.
• High density culture of marine zooplankton, Brachionus plicatilis as initial food organism for aquaculture

  Kenji Tanaka; Hiroyuki Takahashi; Yuji Hamasaki; Takao Yoshimatsu

  Current Topics in Biotechnology 4 41 - 46 2008 [Refereed]
  
• Hydrogen oxidizing bacteria as poly(hydroxybutyrate) producers

  Kainoosh Khosravi Darani; Ebrahim Vasheghani-Farahani; Kenji Tanaka

  Iran. J. Biotechnol. 4 (3) 193 - 196 2007 [Refereed]
  
• The optimization of pollack roe sample solution preparation for determination of nitrite

  Sayaka Soma; Noriko Morinaga; Takako Yoshimoto; Mika Miike; Makoto Nakamura; Kenji Tanaka

  JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI JAPAN SOC FOOD SCIENCE TECHNOLOGY 54 (3) 128 - 132 1341-027X 2007 [Refereed]
   

  Within the standard method for determination of nitrite in processed foods, sample solutions containing nitrite extracted from the foods are treated with sodium hydroxide and zinc acetate, as deproteinizing agents, before colorimetry is conducted by diazotization-coupling. However, in the case of pollack roe, analysis is very difficult or often impossible to carry out due to the turbidity, decrease in filtration rate, and foaming of the sample solution. It is suspected that these conditions are caused by the high concentration of soluble proteins originating from pollack roe. With this in mind, improvement of the procedure for preparing sample solution from pollack roe and its products was investigated. Results showed that decreases in filtration rate were resolved by the use of half the volume of deproteinizing agents in addition to twice the concentration as that of the standard method. The addition of silicone-type anti-foaming agent SI suppressed the sample solution foaming and increased the filtration rate. Adding sodium chloride was not effective in avoiding turbidity or decreasing filtration rate and foaming. Using our modified method for preparing sample solutions, concentration of nitrite in pollack roe was determined to be 0.08 +/- 0.02 ppm and the recovery ratio of 5 ppm of nitrite added to pollack roe was 89.8 +/- 2.5%, respectively.
• Effect of dietary cobalt supplementation on the population growth of rotifer Brachionus rotundiformis

  T Yoshimatsu; T Higuchi; DM Zhang; NR Fortes; K Tanaka; K Yoshimura

  FISHERIES SCIENCE JAPANESE SOC FISHERIES SCIENCE 72 (1) 214 - 216 0919-9268 2006/02 [Refereed]
  
• Effect of Co supplementation on the vitamin B12 production and population growth of rotifer, Brachionus plicatilis: A preliminary report.

  Takao Yoshimatsu; Takahiro Higuchi; Dongming Zhang; Norma R. Fortes; Yuji Hamasaki; Kenji Tanaka; Tsugino Yukino; Masahiro Hayashi

  Reports of School of Humanity-Oriented Science and Engineering, Kinki University 近畿大学産業理工学部 3 (3) 1 - 6 1349-5801 2005
• Effects of cobalt compound supplementation and lighting on population growth of marine rotifer, Brachionus plicatilis

  Takao Yoshimatsu; Takahiro Higuchi; D.M. Zhang; Yuji Hamasaki; Kenji Tanaka; T. Yukino; Masahiro Hayashi

  Proceedings of 11th International Symposium on Nutrition and Feeding in Fish 250  2004
• 海産ワムシの大量培養法とその利用

  田中 賢二

  用水と廃水 46 (5) 3 - 11 2004 [Invited]
  
• A novel culture system for the ultra-high-density production of the rotifer, Brachionus rotundiformis - a preliminary report

  K Yoshimura; K Tanaka; T Yoshimatsu

  AQUACULTURE ELSEVIER SCIENCE BV 227 (1-4) 165 - 172 0044-8486 2003/11 [Refereed]
   

  A novel culture system for continuous filtration of culture water was developed for ultra-high-density production of a marine rotifer. This ultra-high-density culture system is equipped with a membrane filtration unit (pore size: 0.4 mum) set inside a culture vessel. The culture performance of this system was tested by feeding freshwater Chlorella paste as feed in a 4-day batch culture. To prevent the membrane surface from fouling, the filtration unit was cleaned every day by dipping it in a NaOCl solution. The membrane filter unit was effective in exchanging the culture water with a high level of ammonia with fresh seawater, while rotifers and Chlorella cells were retained in the vessel. Using this membrane filtration unit with the supply of oxygen gas, a rotifer density of more than 1.6 x 10(5) individuals ml(-1) was obtained on the 4th day of culture. The removal of accumulated ammonia from the culture water enabled an increase of the productivity and of the vitality of the rotifers. (C) 2003 Elsevier B.V. All rights reserved.
• Development of ultra-high-density culture system for mass production of a zooplankton rotifer as larval fish food by controlling the dissolved oxygen concentration - Monograph

  K Tanaka; K Yoshimura; T Yoshimatsu

  SEIBUTSU-KOGAKU KAISHI SOC BIOSCIENCE BIOENGINEERING JAPAN 81 (3) 112 - 117 0919-3758 2003 [Refereed]
   

  In recent years, the production of larval fish has been rapidly increasing worldwide. A zooplankton, the rotifer Brachionus rotundiformis, plays a very important role as the food of many marine larval fishes used in mariculture and fish cultivation. A typical mariculture corporation requires about 10 billion rotifers every day. However, the culture density of rotifers in the conventional culture method was only ca. 200 individuals (ind.)/ml, which meant large culture tanks of over hundred tons capacity were essential for the production of enough rotifers. Hence, we developed a high-density culture method incorporating the ideas and techniques of microbial engineering. First, condensed freshwater Chlorella were employed as new food for rotifers instead of the bakery yeast and marine Chlorella used in the conventional culture method. Second, we demonstrated that mechanical agitation, a high aeration rate, and the use of a fine porous sparger to attain a high k(L)a were rather inhibitory to rotifer growth. Hence, the feeding of 02 gas was essential to avoid oxygen deficiency. Third, we found that maintaining the pH at 7.0 by automatic addition of HCl to promote the dissociation of NH3 to NH4+ and OH- was very useful in reducing inhibition by NH3. Fourth, a filtration unit was fitted in the culture tank to remove waste matter from the culture liquid. Using these techniques, the culture density of rotifer increased to 20,000 ind./ml. Our high-density culture method is now being used by many mariculture corporations in Japan. Recently, we developed a novel culture system using a continuous membrane filtration unit (CMFU) for ultra-high-density culture. In this culture system, NH4+ and soluble organic substances, which can be nutrients for bacteria, are removed from the culture liquid. Using the CMFU, the rotifer density increased to 160,000 ind./ml and the population of Vibrio sp. inhabiting in the rotifer body decreased to 1/100,000 of that of a culture without CMFU. This new culture system is expected to have great potential for the mass production of sterile rotifer using a culture tank smaller than 100 l capacity.
• Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis 10-1

  K Tanaka; A Komiyama; K Sonomoto; A Ishizaki; SJ Hall; R Stanbury

  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER-VERLAG 60 (1-2) 160 - 167 0175-7598 2002/10 [Refereed]
   

  In lactic acid bacteria, pentoses are metabolized via the phosphoketolase pathway, which catalyzes the cleavage of D-xylulose-5-phosphate to equimolar amounts of glyceraldehyde 3-phosphate and acetylphosphate. Hence the yield coefficient of lactate from pentose does not exceed 1.0 mol/mol, while that of Lactococcus lactis IO-1(JCM7638) at high D-xylose concentrations often exceeds the theoretical value. This suggests that, in addition to the phosphoketolase pathway, L. lactis IO-1 may possess another metabolic pathway that produces only lactic acid from xylose. In the present study, the metabolism of xylose in L. lactis IO-1 was deduced from the product formation and enzyme activities of L. lactis IO-1 in batch culture and continuous culture. During cultivation with xylose concentrations above ca. 50 g/l, the yield coefficient Of L-lactate exceeded 1.0 mol/mol while those of acetate, formate and ethanol were very low. At xylose concentrations less than 5 g/l, acetate, formate and ethanol were produced with yield coefficients of about 1.0 mol/mol, while L-lactate was scarcely produced. In cells grown at high xylose concentrations, a marked decrease in the specific activities of phosphoketolase and pyruvate formate lyase (PFL), and an increase in those of transketolase and transaldolase were ob-served. These results indicate that in L. lactis IO-1 xylose may be catabolized by two different pathways, the phosphoketolase pathway yielding acetate, formate and ethanol, and the pentose phosphate (PP)/glycolytic pathway which converts xylose to L-lactate only. Furthermore, it was deduced that the change in the xylose concentration in the culture medium shifts xylulose 5-phosphate metabolism between the phosphoketolase pathway and the PP/glycolytic pathway in L. lactis IO-1, and pyruvate metabolism between cleavage to acetyl-CoA and formic acid by PFL and the reduction to L-lactate by lactate dehydrogenase.
• Effect of cobalt compound on population growth of rotifer.

  Takao YOSHIMATSU; Takahiro HIGUCHI; Kazuto SHIRAKAWA; Masahiro HAYASHI; Kenji TANAKA; Kenji YOSHIMURA

  Proceeding of the 5th Korea-Japan (Japan-Korea) Biannual Joint Meeting 68  2002
• Ultra-high-density Culture of a marine zooplankton Brachionus rotundiformis (Rotifer) using a membrane filtration

  Kenji Tanaka; Takao Yoshimastu; Kenji Yoshimura

  Proceedings of current biotechnology and Bioengineering (XI),Korea. p514. 514  2002
• Ultra-high-density culture of the rotifer Brachionous rotundiformis using a membrane filtration unit and its effect on bacterial abundance in the rotifer body

  K Tanaka; N Takeda; Y Minami; M Kuramoto; K Yoshimura; T Yoshimatsu

  SEIBUTSU-KOGAKU KAISHI SOC BIOSCIENCE BIOENGINEERING JAPAN 80 (5) 181 - 187 0919-3758 2002 [Refereed]
   

  We have developed a novel culture system designed for ultra-high-density production of the so-called S-type marine rotifer (Brachionus rotundiformis), which has become an indispensable initial food item for larval cultures of marine fishes and crustaceans. In our culture system, a membrane-type micro-filtration unit (pore size was 0.4 mum) was incorporated in a culture tank (40 l) to prevent accumulation of NH4+, which is a strong inhibitor of rotifer growth. In rotifer batch culture tests with and without the culture system, freshwater Chlorella paste (Chlorella regularis; packed vol., 600 ml/l) was fed continuously into the culture water using a peristaltic tube pump at feed rates from 3.8 - 8.0 l/tank/d. Oxygen gas (purity, 90%) was fed at a rate of 0.2 vvm. In the batch culture system with the filtration unit, the culture broth was filtered out through the unit and fresh seawater was supplied to maintain the culture volume in the tank. In batch culture without filtration, the rotifer density reached 40,000 rotifers/ml after 2 d. However, the rotifer density subsequently seriously decreased due to an increase in the NH4+ concentration up to 900 ppm. In contrast, in the culture with filtration the NH4+ concentration remained below 240 ppm after 4 d, and as a result the rotifer density reached 160,000 ind./ml. The population of Vibrio sp, in the rotifer body diminished from 10(9) to 10(3) CFU/g-rotifer as a consequence of using the filtration unit.
• Behavior and control of bacterial flora in high density culture of rotifer

  K Tanaka

  NIPPON SUISAN GAKKAISHI JAPANESE SOC FISHERIES SCIENCE 67 (6) 1142 - 1143 0021-5392 2001/11 [Refereed]
  
• Microbial production of poly-D-3-hydroxybutyrate from CO(2)

  A Ishizaki; K Tanaka; N Taga

  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY SPRINGER 57 (1-2) 6 - 12 0175-7598 2001/10 [Refereed]
   

  This short review covers the biotechnological aspects of the production Of poly-D-3-hydroxybutyric acid, P(3HB), from H(2), O(2) and CO(2) by autotrophic culture of the hydrogen-oxidizing bacterium, Ralstonia eutropha. Considering the efficiency of utilization of a gas mixture as substrate, a practical fermentation process using R. eutropha for the mass production of P(3HB) from CO(2) should be designed on the basis of a recycled-gas, closed-circuit culture system. Also, maintaining the O(2) concentration in a gas phase lower than 6.9% (v/v) is essential to prevent the gas mixture from exploding. Our study, using an explosion-proof fermentation bench plant and a two-stage culture system with a newly designed air-lift fermenter, demonstrated that very high P(3HB) yield and productivity could be obtained while the O(2) concentration was maintained below 6.9%. However, a study on the continuous production of P(3HB) from CO(2) by chemostat culture of R. eutropha revealed that the productivity and content of P(3HB) in the cells was considerably lower than by fed-batch culture. It is deduced that the use of the hydrogen-oxidizing bacterium, Alcaligenes latus, which accumulates P(3HB) even in the exponential growth phase, will be useful for the effective production of P(3HB) from CO(2).
• Development of a novel method for feeding a mixture of L-lactic acid and acetic acid in fed-batch culture of Ralstonia eutropha for poly-D-3-hydroxybutyrate production

  T Tsuge; K Tanaka; A Ishizaki

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 91 (6) 545 - 550 1389-1723 2001/06 [Refereed]
   

  Fermentative production Of poly-D-3-hydroxybutyrate [P(3HB)] from a mixture of L-lactic acid and acetic acid by Ralstonia eutropha was investigated. For fed-batch culture with high cell density, it is necessary to control the concentration of these organic acids in the culture medium below the inhibitory level for cell growth. Therefore, a novel feeding method, termed the computer-controlled pH-stat substrate feeding method, was developed using the rate of increase of the pH (pH-increasing rate) of the culture medium as an indicator for feed control. The pH-increasing rate, which was calculated every minute by a pH meter-linked computer, represented secondary information regarding substrate consumption by cells. When the pH-increasing rate decreased to 5% of the maximum increasing rate, acidic substrate solution was fed into the fermentor until the pH was reduced to 7.00. Using this feeding strategy, the cell concentration and PHA content obtained in 42 h were 75.0 g/l and 73.1% (w/w), respectively, resulting in a high P(3HB) productivity of 1.30 g/l(.)h.
• Ultrahigh-density Culture System of the Rotifer, Brachionus rotundiformis. Yoshimatsu Takao

  Yoshimatsu Takao; Kenji Yoshimura; Kenji Tanaka

  Proceedings of 3rd International Fish & Shellfish Larviculture Symposium "Larvi 2001", Belgium 638 - 639 2001
• High Density Mass-culture of Rotifer by Feeding Yeast Cream and Condensed Freshwater Chlorella

  Yuzuru Abe; Kenji Yoshimura; Takashi Soda; Shuichi Sakamoti; Takao Yoshimatsu; Kenji Tanaka

  Suisan Zoushoku Japanese Society for Aquaculture Science 49 (4) 489 - 494 0371-4217 2001 [Refereed]
   

  Factory-made, condensed raw paste of baker's yeast, Saccharomyces cerevisiae, i.e. yeast cream was introduced as a culture feed in the effort to reduce the production cost of the rotifer, Brachionus rotundiformis in high density culture system. Although a sole use of the yeast cream could not attain stable rotifer growth, the maximum combination ratio of yeast cream to Chlorella paste successful in high density culture of rotifer was obtained in the case of fifty-fifty in the laboratory scale culture. Semi-continuous culture in the practical scale through 30 days was conducted with feeding 20 l/day of Chlorella paste during the lst-10th day and the 50% replacement of it with yeast cream during the 11th-30th day. During the test period, daily density and PV (packed volume) of rotifer were almost stable at 8×10³ ind./ml and 8 ml/l, respectively. The yield co-efficiency of rotifer production was not different between for the first 10 days (39.4×10⁸ind. /kl/day) and for the next 20 days (40.1×10⁸ ind. /kl/day). The cost of feed for the production of 10⁸ individuals of rotifer was 381 yen for the first 10 days and 313 yen for the next 20 days, decreasing by 20% with feeding yeast cream. Moreover, concentration of total ammonia in the culture medium and lorica length of rotifers were not changed with composition of the feed.
• ワムシ培養方法の類別と培養事例

  吉村研治; 吉松隆夫; 田中賢二

  月刊養殖 緑書房 479, (10) 90 - 95 0044-0671 2001 [Invited]
  
• Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method

  G Kobayashi; K Tanaka; H Itoh; T Tsuge; K Sonomoto; A Ishizaki

  BIOTECHNOLOGY LETTERS KLUWER ACADEMIC PUBL 22 (13) 1067 - 1069 0141-5492 2000/07 [Refereed]
   

  The feeding of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] by Alcaligenes eutrophus ATCC17697 was optimized using a fed-batch culture system. The concentration of propionic acid was maintained at 3 g l(-1) as growth was inhibited by propionic acid in the broth. A pH-stat substrate feeding system was used in which propionic acid was fed automatically to maintain a pH of the culture broth at 7.0. By feeding a substrate solution containing 20% (w/v) propionic acid, 4.9% (w/v) ammonia water [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10] in cell growth phase, the concentration of propionic acid in the broth was maintained at 3 g l(-1) giving a specific growth rate of 0.4 h(-1). To promote P(3HB-co-3HV) production, two stage fed-batch culture which consisted of the stage for the cell growth and the stage for the P(3HB-co-3HV) accumulation was carried out. When the substrate solution whose C/N molar ratio was 50 was fed in P(3HB-co-3HV) accumulation phase, the cell concentration and the P(3HB-co-3HV) content in the cells reached 64 g l(-1) and 58% (w/w) in 55.5 h, respectively.
• The fermentation characteristics of hydrogen-oxidizing bacterium, Ralstania eutropha and cultivation system to produce poly-D-3-hydroxybutyrate from CO2.

  Kenji Tanaka; Toshihiro Takeshita; Ayaaki Ishizaki

  Res.Adv. in Biosci.& Bioeng. 1 7 - 17 2000 [Refereed]
  
• Optimization of L-lactic acid feeding for the production of poly-D-3-hydroxybutyric acid by Alcaligenes eutrophus in fed-batch culture

  T Tsuge; K Tanaka; M Shimoda; A Ishizaki

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 88 (4) 404 - 409 1389-1723 1999/10 [Refereed]
   

  We investigated optimization of the feeding of L-lactic acid for the production of poly-D-3-hydroxybutyric acid [P(3HB)] by Alcaligenes eutrophus in a fed-batch culture system, An acidic substrate solution was fed automatically so as to maintain the pH of the culture liquid at 7.0. Feeding of a substrate solution containing 45% (w/v) L-lactic acid, 6.2% (w/v) sodium L-lactate, 5.8% (w/v) ammonia water and 1.8% (w/v) potassium phosphate [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10], allowed the L-lactate concentration in the culture liquid to be maintained at approximately 2 g/l and the cell concentration reached 27.4 g/l after 15 h of cultivation, To promote P(3HB) production, a two-stage fed-batch culture consisting of a culture for cell growth and one for P(3HB) accumulation was carried out. When the substrate solution, whose C/N molar ratio was 23, was fed during the P(3HB) accumulation phase, the cell concentration and the P(3HB) content in the cells reached 103 g/l and 57.6% (w/w), respectively, in 51.5 h.
• Investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by Zymomonas mobilis

  K Tanaka; ZD Hilary; A Ishizaki

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING SOC BIOSCIENCE BIOENGINEERING JAPAN 87 (5) 642 - 646 1389-1723 1999/05 [Refereed]
   

  The utility of the juice of rotten or discarded pineapples and the waste material of the production of pineapple juice as low-cost substrates for ethanol production by Zymomonas mobilis was investigated. Z. mobilis ATCC 10988 produced 59.0 g.l(-1) ethanol in undiluted pineapple juice without nutritional supplementation and without the regulation of the pH while 42.5 g.l(-1) ethanol was obtained using a 125 g.l(-1) sucrose medium supplemented with 10 g.l(-1) yeast extract and mineral salts. Ethanol fermentation using unhydrolyzed and enzymatically hydrolyzed pineapple waste material was also investigated under various culture conditions. When a 15% (v/v) dilution of unhydrolyzed waste material without nutritional supplementation was used, more than 3.5 g.l(-1) ethanol was produced. When the media containing 15, 30, and 40% (v/v) of the hydrolyzate consisting of a 60% (v/v) suspension of pineapple waste material were used, final concentrations of ethanol were 5.0 g.l(-1), 7.6 g.l(-1), and 9.3 g.l(-1), respectively. These results suggest that pineapple juice and the waste material can be useful low-cost substrates for ethanol production by Z. mobilis without supplementation with expensive organic nitrogen complexes such as yeast extract and without the regulation of the pH during cultivation, leading to the reduction in the production costs.
• Development of high efficient L-lactate fermentation and P(3 HB) biosynthesis for "lactate industry", the new technology for post-petrochemistry

  A Ishizaki; G Kobayashi; P Vonktaveesuk; T Tsuge; K Tanaka

  KAGAKU KOGAKU RONBUNSHU SOC CHEMICAL ENG JAPAN 25 (2) 136 - 142 0386-216X 1999/03 [Refereed]
   

  We have developed a high efficiency L-lactate fermentation process employing the homofermentative lactic acid bacterium, Lactococcus lactis IO-1 with a pH-dependent substrate feed system in order to consume the substrate completely and maintain high activity of cell growth, production, and substrate consumption. Furthermore a pH-dependent substrate feed system was combined with turbidity control consisting of a laser probe and micro filter module for cell-recycling. With this system, we achieve a high efficiency L-lactate continuous culture. We are able to construct high efficiency bioconversion systems from xylose to P (3 HE) via L-lactate and other organic acids because Lactococcus lactis IO-1 can utilize not only glucose but also xylose as a carbon source. Thus, we have developed a new substrate feed system which is able to automatically control substrate concentration in a broth. A new batch fermentative system for production of biodegradable plastic material, polyhydroxybutyrate [P (3 HE)] from culture broth of Lactococcus lactis IO-1 is vealized.
• Control of acetic acid concentration by pH-Stat continuous substrate feeding in heterotrophic culture phase of two-stage cultivation of Alcaligenes eutrophus for production of P(3HB) from CO2, H-2, and O-2 under non-explosive conditions

  T Sugimoto; T Tsuge; K Tanaka; A Ishizaki

  BIOTECHNOLOGY AND BIOENGINEERING JOHN WILEY & SONS INC 62 (6) 625 - 631 0006-3592 1999/03 [Refereed]
   

  A-two stage culture method of hydrogen-oxidizing bacterium, Alcaligenes eutrophus, is used to produce poly-D-3-hydroxybutyrate, P(3HB) from CO2, O-2, and H-2 without using a very high oxygen transfer rate while maintaining the O-2 concentration in gas phase below 6.9 (v/v)% to prevent detonation of the gas mixture. The two-stage method consists of a heterotrophic culture using fructose as carbon source for exponential cell growth and an autotrophic culture for P(3HB) accumulation. We investigated the use of acetic acid as a cheaper carbon source than fructose for the heterotrophic culture in the two-stage method. However, the acetate concentration in the culture system must be maintained at 1.0 g . dm(-3) since its inhibitory effect on the cell growth is very strong. Then, high cell density cultivation of A. eutrophus was investigated by pH-stat continuous feeding of acetic acid to control acetate concentration. As a result, acetate concentration was automatically maintained around 1.0 g dm(-3) by using a feed with a composition in CH3COOH/CH3COONH4/KH2PO4 molar ratio of 5:1:0.084. Cell concentration increased to 48.6 g . dm(-3) after 21 h of cultivation. The cell mass grown in the fed-batch culture on acetic acid was useful for P(3HB) production from CO, in the subsequent autotrophic culture stage. (C) 1999 John Wiley & Sons, Inc.
• Preliminary investigation of the effect of the use of pineapple juice and the waste on ethanol production by Zymomonas mobilis

  ZD Hilary; K Tanaka; A Ishizaki

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY FACULTY AGRICULTURAL PUBL 43 (3-4) 433 - 439 0023-6152 1999/02 

  The effect of the use of the juice of rotten-or discharged pineapple fruits and the wastes derived from the processing of pineapple juice on ethanol production by Z. mobilis was investigated. Z. mobilis ATCC 10988 produced 59.0 gl(-1) of ethanol in the undiluted pineapple juice although the supplementation of any nutritions and the controlling of pH were not carried out. Unhydrolyzed waste and the enzymatically hydrolyzed waste were also converted to ethanol at the relatively high yield coefficients. These results suggest tl-lat pineapple juice and the waste can be useful for ethanol production by Z. mobilis. Further, it was shown that the use of expensive organic-nitrogen complex such as yeast extract and the controlling of pH can be omitted from the fermentation process using pineapple juice and the waste.
• Changes in the Concentrations of Ammonia and Particulate Organic Matter and Rotifer Biomass in High Density Semi-Continuous Culture

  Kenji Yoshimura; Takashi Usuki; Takao Yoshimastu; Kenji Tanaka; Ayaaki Ishizaki; Hideki Uemura

  Suisan Zoushoku 水産増殖談話会 46 (2) 183 - 192 0371-4217 1998 [Refereed]
  
• Quantitative determination and separation of wastes in high density culture medium for marine rotifer

  K Yoshimura; K Usuki; T Yoshimatsu; K Tanaka; A Ishizaki

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES SCI 63 (6) 912 - 919 0021-5392 1997/11 [Refereed]
   

  In high density culture of rotifer, a large amount of suspended organic matter accumulates in the culture medium. Such wastes are mainly composed of rotifer feces, amictic egg shells, microbes (bacteria, protozoa, fungi etc.), the food organism Chlorella and flocks of various sizes are formed and coagulate. The increase of flocks in culture medium causes clogging of the nets in harvesting of rotier and also causes pollution of the larval rearing tank by providing pathogenic microorganisms. In order to remove the hocks, a filtration unit was developed, which was composed of nylon filtration mats and stainless steel frames to aid mixing of the culture medium. In order to investigate its efficiency, changes in the quantities of rotifer and wastes (the large amount of suspended organic matter) were determined by the centrifuge method. The culture of rotifer was carried out in two 500 l tanks by the semi-continuous method with or without filtration for five and nine days. While the flocks increased in the culture medium without filtration, the hocks larger than 30 mu m could be efficiently removed with filtration and culture was feasible at a rotifer density of 10(4) ind./ml.
• Effects of rheological change by addition of carboxymethylcellulose in culture media of an air-lift fermentor on poly-D-3-hydroxybutyric acid productivity in autotrophic culture of hydrogen-oxidizing Bacterium, Alcaligenes eutrophus

  N Taga; K Tanaka; A Ishizaki

  BIOTECHNOLOGY AND BIOENGINEERING JOHN WILEY & SONS INC 53 (5) 529 - 533 0006-3592 1997/03 [Refereed]
   

  The effects of rheological change by addition of sodium carboxymethylcellulose (CMC) to culture medium in an air-lift-type fermenter on autotrophic production of poly-(D-3-hydroxybutyric acid) [P(3HB)] by two-stage culture of Alcaligenes eutrophus is investigated. Addition of 0.05% CMC increased P(3HB) production rate during the P(3HB) accumulation phase to twice that of the control culture. It was thought that addition of a small amount of CMC was beneficial for production of P(3HB) employing the air-lift fermenter under safe autotrophic culture conditions in wich oxygen concentration was maintained below 6.9% (v/v). the volumetric mass transfer coefficient (K(L)a) observed in the presence of CMC is shown to correlated with the P(3HB) production rate obtained. (C) 1997 John Wiley & Sons, Inc.
• Microbial production of biodegradable plastics from carbon dioxide and agricultural waste material

  A Ishizaki; N Taga; T Takeshita; T Sugimoto; T Tsuge; K Tanaka

  FUELS AND CHEMICALS FROM BIOMASS AMER CHEMICAL SOC 666 294 - 306 0097-6156 1997 [Refereed]
   

  Fermentative production of biodegradable plastic material, poly-D-3-hydroxybutyrate, P(3HB) from CO2 or agricultural wastes is expected to contribute to the solution of global environmental pollution problems. The practical cultivation systems to produce P(3HB) from CO2, H-2 and O-2 by hydrogen-oxidizing bacterium were developed by maintaining O-2 concentration in gas phase below the lower limit for explosion. P(3HB) productivity was increased by improving gas mass transfer with the use of air-lift fermenter and the addition of 0.05 %CMC to the culture medium. P(3HB) was also produced from xylose via L-lactate by two-stage culture method using Alcaligenes eutrophus and Lactococcus lactis IO-1. P(3HB) productivity was increased by a pH-stat fed-batch culture method with feeding the substrate solution so as to control L-lactate concentration at very low level.
• Development of fermentation processes for autotrophic production of P(3HB) from CO2 under safe culture conditions

  K Tanaka

  SEIBUTSU-KOGAKU KAISHI-JOURNAL OF THE SOCIETY FOR FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 75 (3) 185 - 195 0919-3758 1997 [Refereed]
   

  Fermentative production of a biodegradable plastic material, poly-D-3-hydroxybutyrate, P(3HB) from CO2 is expected to contribute to the solution of global environmental pollution problems caused by the increased CO2 concentration in the atmosphere and the disposal of non-degradable plastic materials. Practical culturemethod for autotrophic production of P(3HB) from CO2, H-2 and O-2 by the hydrogen-oxidizing bacterium Alcaligenes eutrophus were investigated. A recycled-gas closed-circuit culture system enabled high-cell-density cultivation with high utilization efficiency of substrate gasses. The fermentation conditions for cell growth and P(3HB) accumulation and the stoichiometry for biomass production were thus investigated. The P(3HB) concentration could be increased to 91.3 g/l after 40 h cultivation using an explosion-proof type of fermentation system without the danger of substrate-gas explosion by maintaining the O-2 concentration in the gas phase below the lower limit for explosion (6.9%) and by using a high-performance agitation system (KLa=2,970 h-1). In order to produce P(3HB) under a non-explosive gas composition without using a highperformance agitation system, a two-stage culture method was developed. This method consisted of a heterotrophic cell-growth stage using an organic carbon source and an autotrophic P(3HB) accumulation stage. The P(3HB) concentration could be increased to 21 g/l in the autotrophic culture stage using this method in a normal type of jar fermenter (KLa=340 h(-1)) under a safe gas composition.
• Determination of Marine Rotifier Biomass by Centrifugation

  Kenji Yoshimura; Takashi Usuki; Takao Yoshimatsu; Kenji Tanaka; Ayaaki Ishizaki

  Suisan Zoushoku 45 (2) 171 - 177 0371-4217 1997 [Refereed]
  
• L-乳酸からのP(3HB)生産

  生物工学会誌 75 380 - 382 1997 [Refereed]
  
• On the aeration method in high density culture of rotifer Brachionus rotundiformis

  K Yoshimura; Y Ohmori; T Yoshimatsu; K Tanaka; A Ishizaki

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 62 (6) 897 - 903 0021-5392 1996/11 [Refereed]
   

  In a high density culture of rotifer (beyond the levels of 10(3) ind./ml), the amount of aeration needed to maintain an optimum dissolved oxygen (DO) level increases. In order to investigate the effect of aeration on the population growth of rotifer, a series of experiments were carried out. The S-type rotifer Brachionus rotundiformis was cultured at high density using 10 l vessels under different aeration conditions. Air or oxygen gas was introduced into the culture media through two types of sparger which formed different sizes of bubbles, at aeration rates from 0.1 to 2.0 vvm. The bubble-size from the two kinds of sparger were estimated from the value of KLa (volumetric oxygen transfer coefficient). When the aeration rate was high or the ceramic sparger that formed smaller bubbles was used, DO in the culture system remained at a high level. However, under these conditions, the growth of the rotifer population was seriously inhibited due to very high aeration rates and small bubbles, possibly as a result of foaming separation. When oxygen gas was introduced into the culture system, the density of rotifer was higher than that when air was introduced. Therefore, it is recommended to introduce high-purity oxygen gas for a high density culture to avoid any growth inhibition and to maintain optimum DO.
• Continuous cultures with xylose for lactic acid production by Lactococcus lactis IO-1

  Chainanrong Rattanakreetakul; Kenji Tanaka; Ayaaki Ishizaki

  ICBiotech 19 150 - 160 1996
• Effects of rheologic conditions in the culture medium on the autotrophic production of poly-(D-3-hydroxybutyric acid) in an air-lift fermenter

  N Taga; K Tanaka; A Ishizaki

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY FACULTY AGRICULTURAL PUBL 40 (1-2) 197 - 207 0023-6152 1995/12 

  The effects of rheological changes in the culture medium, caused by addition of sodium carboxymethylcellulose (CMC), on the autotrophic production of poly-(D-3-hydroxybutyric acid) [P(3HB)] by Alcaligenes eutrophus was investigated in an air-lift fermentor. Addition of 0.05% CMC increased P(3HB) productivity to twice as in the control culture. The effect of CMC on the volumetric mass transfer coefficient (K-L alpha) and the relationship between K-L alpha and P(3HB) productivity were investigated. The production of P(3HB) rate was not correlated with K-L alpha, when K-L alpha was measured by the sulfite oxidation method, but it was correlated with K-L alpha when K-L alpha was measured by the static method. Thus, the sulfite oxidation method was unsuitable for measurements of the K-L alpha of this system.
• A HIGH-DENSITY CULTIVATION OF ROTIFER IN AN ACIDIFIED MEDIUM FOR REDUCING UNDISSOCIATED AMMONIA

  K YOSHIMURA; T IWATA; K TANAKA; C KITAJIMA; F ISIZAKI

  NIPPON SUISAN GAKKAISHI JAPAN SOC SCI FISHERIES TOKYO UNIV FISHERIES 61 (4) 602 - 607 0021-5392 1995/07 [Refereed]
   

  Two experiments (Exp. I: batch method using 1 l vessels and Exp. II: semi-continuous method using 200 l tanks) were carried out in order to investigate the effect of acidification of the media in high density cultures of the rotifer Brachionus rotundiformis on the reduction of poisonous undissociated ammonia. Rotifer were cultured in media with pH adjusted to 6, 7 and 8, and without pH adjustment, bubbling oxygen gas and feeding commercial condensed Chlorella regularis. The pH was adjusted with the automatic addition of HCl (35%) and NaOH (10 N) through a pH controller. The concentration of undissociated ammonia in the medium without pH adjustment (Exp. I) and of 8 pH (Exp. II) increased to 27 and 16 ppm, and the growths of rotifer population were inactive and their maximum densities mere 8,000 and 3,000 individ./ml, respectively. On the other hand, the concentration of undissociated ammonia in the medium of 7 pH remained less than 10 ppm, and in the medium of 6 pH, it was only 2 ppm. The growths of rotifer population in their media were remarkably active, and their maximum densities reached 22,000 to 34,000 individ./ml (Exp. I) and 9,000 to 13,000 individ./ml (Exp. II).
• EFFECT OF NITROGEN-SOURCES IN CULTURE-MEDIUM ON L-LACTATE FERMENTATION EMPLOYING LACTOCOCCUS-LACTIS IO-1

  K TANAKA; T OHTA; A ISHIZAKI

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY FACULTY AGRICULTURAL PUBL 39 (3-4) 131 - 138 0023-6152 1995/03 

  Effect of amino acids, yeast extract and polypeptone on cell growth and L-lactate production of Lactococcus lactis IO-1 was investigated. The growth rate of the microorganism, cultivated in a synthetic medium consisting of amino acid, vitamins and minerals, is lower than that of the media containing yeast extract. It was considered that yeast extract was essential for rapid growth of L. lactis IO-1. An increase in yeast extract concentration in the culture medium increased specific growth rate and final cell concentration of L. lactis IO-1, and as a result the fermentation time was shortened.
• PRODUCTION OF POLY(D-3-HYDROXYBUTYRATE) FROM CO2, H-2, AND O-2 BY HIGH CELL-DENSITY AUTOTROPHIC CULTIVATION OF ALCALIGENES-EUTROPHUS

  K TANAKA; A ISHIZAKI; T KANAMARU; T KAWANO

  BIOTECHNOLOGY AND BIOENGINEERING JOHN WILEY & SONS INC 45 (3) 268 - 275 0006-3592 1995/02 [Refereed]
   

  Hydrogen-oxidizing bacterium, Alcaligenes eutrophus autotrophically produces biodegradable plastic material, poly(D-3-hydroxybutyrate), P(3HB), from carbon dioxide, hydrogen, and oxygen. In autotrophic cultivation of the microorganism, it is essential to eliminate possible occurrence of gas explosions from the fermentation process. We developed a bench-plant scale, recycled-gas, closed-circuit culture system equipped with several safety features to perform autotrophic cultivation of A. eutrophus by maintaining the oxygen concentration in the substrate gas phase below the lower limit for a gas explosion (6.9%). The culture vessel utilized a basket-type agitator, resulting in a K(L)a value of 2970 h-1. Oxygen gas was also directly fed to the fermenter separately from the other gases. As a result, 91.3 g . dm(-3) of the cells and 61.9 g . dm(-3) of P(3HB) were obtained after 40 h of cultivation under this oxygen-limited condition. The results compared favorably with those reported for mass production of P(3HB) by heterotrophic fermentation. (C) 1995 John Wiley and Sons, Inc.
• Fermentative production of poly--hydroxybutyric acid from xylose via L-lactate by a two stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

  Kenji Tanaka; Kouji Katamune; Ayaaki Ishizaki

  Can. J. Microbiol. 41(suppl.1) (257) 261  1995 [Refereed]
  
• 微生物による炭酸ガスの資源化

  石崎文彬; 田中賢二; 金丸利壽

  学術月報 48 1235  1995 [Invited]
  
• PRODUCTION OF POLY-D-3-HYDROXYBUTYRIC ACID FROM CARBON-DIOXIDE BY A 2-STAGE CULTURE METHOD EMPLOYING ALCALIGENES-EUTROPHUS ATCC 17697(T)

  K TANAKA; A ISHIZAKI

  JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 77 (4) 425 - 427 0922-338X 1994 [Refereed]
   

  A two stage culture was examined as a new method for the cultivation of Alcaligenes eutrophus to produce poly-D-3-hydroxybutyric acid, P(3HB), from H-2, O-2 and CO2 without danger of gas explosion. In this culture method, the microorganism was grown beforehand in an organic medium under heterotrophic conditions for exponential growth, and the cells were then cultivated for P(3HB) accumulation under autotrophic conditions in which the O-2 concentration in the substrate gas was below the explosion limit of 6.9%. Although the K-L alpha of the fermenter used was not high (ca. 340 h(-1)), it was possible to obtain P(3HB) at a relatively high production rate (0.56-0.91 g.dm(-3).h(-1)) and concentration (15.23-23.91 g dm(-3)) in the autotrophic stage, even under such a low O-2 concentration.
• Fermentative production of L-lactate from xylose

  T Ueda; K Tanaka; A Ishizaki

  DEVELOPMENTS IN FOOD ENGINEERING, PTS 1 AND 2 BLACKIE ACADEMIC & PROFESSIONAL 552 - 554 1994
• 木材資源廃棄物からエコマテリアルを生産する微生物プロセスに関する研究

  田中 賢二

  日産科学振興財団研究報告書 18 129 - 132 1994
• STUDIES ON DISSOLVED HYDROGEN BEHAVIOR IN AUTOTROPHIC CULTURE OF ALCALIGENES-EUTROPHUS ATCC-17697

  T TAKESHITA; K TANAKA; A ISHIZAKI; PF STANBURY

  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY FACULTY AGRICULTURAL PUBL 38 (1-2) 55 - 64 0023-6152 1993/12 

  The partial pressure of dissolved hydrogen was measured to investigate the behavior of dissolved hydrogen during the autotrophic growth of the hydrogen-oxidizing bacterium, Alcaligenes eutrophus ATCC 17697(T). A dissolved hydrogen sensor was constructed by modifying a Clark type dissolved oxygen probe. By using this sensor the overall volumetric coefficient of hydrogen mass transfer, (K(L)a)(H2), was determined in the culture system and compared with that of oxygen, (K(L)a)(O2biol). Good straight line relationships were demonstrated between (1) 1n (K(L)a)(O2biol). and 1n (K(L)a)(O2sul). (K(L)a for oxygen determined by sulphite oxidation) and (2) 1n (K(L)a)(O2biol). and 1n (K(L)a)(H2). The critical partial pressure for dissolved hydrogen was shown to be 11.6kPa, while that for oxygen was 3.17 kPa. The Kd (volumetric absorption coefficient) values for hydrogen and oxygen were determined from the respective K(L)a's under a range of operating conditions and whereas the K(L)a values for hydrogen were greater than those for oxygen the Kd values were very similar.
• Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

  Kenji Tanaka; Kouji Katamune; Ayaaki Ishizaki

  Biotechnology Letters Kluwer Academic Publishers 15 (12) 1217 - 1222 0141-5492 1993/12 [Refereed]
   

  A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB. © 1993 Science and Technology Letters.
• THE KINETICS OF END-PRODUCT INHIBITION OF L-LACTATE PRODUCTION FROM XYLOSE AND GLUCOSE BY LACTOCOCCUS-LACTIS IO-1

  A ISHIZAKI; T UEDA; K TANAKA; PF STANBURY

  BIOTECHNOLOGY LETTERS CHAPMAN HALL LTD 15 (5) 489 - 494 0141-5492 1993/05 [Refereed]
   

  The relative contributions of lactate inhibition and the generation of sterile (undividing) cells to the low xylose utilisation rate of Lactococcus lactis IO-1 was investigated. The lactate inhibition constant of xylose grown cells was shown to be 9.3 times more than that of glucose grown cells. However, the sterile cell production rate and LDH inactivation rate of the xylose cultures were at least 10 times less than the glucose cultures. Thus, it is suggested that the slower substrate consumption rate in xylose medium is caused mainly by the large inhibition constant for the end product.
• EQUIPMENT AND OPERATION FOR FERMENTATIVE PHB PRODUCTION USING GASEOUS SUBSTRATE TO GUARANTEE SAFETY FROM EXPLOSION

  A ISHIZAKI; K TANAKA; T TAKESHITA; T KANEMARU; T SHIMOJI; T KAWANO

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN SOC CHEMICAL ENG JAPAN 26 (2) 225 - 227 0021-9592 1993/04 [Refereed]
  
• DEVELOPMENT OF A DISSOLVED HYDROGEN SENSOR AND ITS APPLICATION TO EVALUATION OF HYDROGEN MASS-TRANSFER

  T TAKESHITA; K TANAKA; A ISHIZAKI; PF STANBURY

  JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 76 (2) 148 - 150 0922-338X 1993 [Refereed]
   

  The partial pressure of dissolved hydrogen was measured by a dissolved hydrogen sensor constructed by modifying a Clark-type dissolved oxygen probe. Using this sensor, the overall volumetric coefficient of hydrogen mass transfer, (K(L)a)H2, was determined in the culture system and compared with that of oxygen, (K(L)a)O2biol.. Good straight line relationships were demonstrated between (i) In(K(L)a)O2biol. and In(K(L)a)O2sul. (K(L)a for oxygen determined by sulphite oxidation) and (ii) In(K(L)a)O2biol. and In(K(L)a)H2.
• L-LACTATE PRODUCTION FROM XYLOSE EMPLOYING LACTOCOCCUS-LACTIS IO-1

  A ISHIZAKI; T UEDA; K TANAKA; PF STANBURY

  BIOTECHNOLOGY LETTERS CHAPMAN HALL LTD 14 (7) 599 - 604 0141-5492 1992/07 [Refereed]
   

  The growth, substrate utilisation and L-lactate production of Lactococcus lactis IO-1 were examined on xylose, and glucose and xylose media. The yield of lactate on xylose was 0.47 g lactate / g xylose at an initial xylose concentration of 51.2 g /l and the mu(max) was 0.72 h-1. Xylose cultures were more susceptible to lactate inhibition than were glucose cultures but showed similar kinetic behaviour. The organism was capable of complete sugar utilisation when grown on a mixture of 20 g /l xylose and 20 g /l glucose and synthesised 0.66 g lactate / g sugar.
• ACCUMULATION OF POLYPHOSPHATE AND SUBSTRATE GAS UTILIZATION EFFICIENCY IN PHB ACCUMULATION PHASE OF AUTOTROPHIC BATCH CULTURE OF ALCALIGENES-EUTROPHUS ATCC17697T

  K TANAKA; A ISHIZAKI; PF STANBURY

  JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 74 (5) 288 - 291 0922-338X 1992 [Refereed]
   

  The consumption of phosphate, formation of polyphosphate and the intracellular ATP level were compared in exponential phase and PHB-accumulating cells of Alcaligenes eutrophus. The phosphate consumption (mg per g dry cell weight) by PHB-accumulating cells was larger than that by exponentially growing cells. The phosphate consumed by the PHB accumulating cells was converted to polyphosphate, which was a storage substance for phosphorus. The intracellular ATP concentration in PHB-accumulating cells (expressed as mumol g-1 protein) was 1.5 times that in exponentially growing cells. The same proportional increase in H-2 oxidation was observed. From this result it was observed that the ATP pool size expressed in terms of ATP/g protein per mol H-2 product was the same in both culture phases.
• Production of PHB from carbon dioxide employing Alcaligenes eutrophus ATCC 17697T using a two stage culture system

  Ayaaki Ishizaki; Kenji Tanaka

  FEMS Microbiology Reviews 103 412  1992 [Refereed]
  
• PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID FROM CARBON-DIOXIDE BY ALCALIGENES-EUTROPHUS ATCC 17697T

  A ISHIZAKI; K TANAKA

  JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 71 (4) 254 - 257 0922-338X 1991 [Refereed]
   

  The characteristics of PHB production from carbon dioxide by autotrophic culture of Alcaligenes eutrophus ATCC 17697T using a recycled gas closed circuit culture system under the condition of oxygen limitation were investigated. Cell concentration increased to more than 60 g/l after 60 h of cultivation, while the PHB concentration reached 36 g/l. PHB accumulation in the oxygen-limited culture was superior than that in an ammonium-deficient culture. The PHB produced was identified as a homopolymer of D-3-hydroxybutyrate by H-1 and C-13 NMR analysis. The stoichiometry for PHB production from CO2 under the oxygen limitation condition was indicated to be as follows: 33H2 + 12O2 + 4CO2 --> C4H6O2 + 30H2O. This stoichiometry shows that the hydrogen consumption per one mole of CO2 for PHB production is larger than that for cell formation.
• BATCH CULTURE OF ALCALIGENES-EUTROPHUS ATCC 17697T USING RECYCLED GAS CLOSED-CIRCUIT CULTURE SYSTEM

  A ISHIZAKI; K TANAKA

  JOURNAL OF FERMENTATION AND BIOENGINEERING SOC FERMENTATION BIOENGINEERING, JAPAN 69 (3) 170 - 174 0922-338X 1990 [Refereed]
  
• Production of biopolymer for biodegradable plastic from carbon dioxide by using new fermentation system for gaseous substrate

  Ayaaki Ishizaki; Kenji Tanaka

  The proceeding for global environment and energy issues, Fukuoka International Symposium 1990 137 - 143 1990

Books etc

• 脱石油に向けたCO2資源化技術 ―化学的・生物学的利用法を中心に－

  田中賢二; 福居 俊昭 (Contributor遺伝子組換え水素酸化細菌によるCO2からの共重合ポリエステルの生合成. pp. 323-330)シーエムシー出版 2020/07
• 農芸化学の事典（鈴木昭憲、荒井綜一編）, エネルギー代謝

  田中 賢二 (Single work)朝倉書店 2003/10 

  農芸化学における基礎領域のうち、微生物のエネルギー獲得機構全般を担当・解説した。
• エネルギー代謝。農芸化学ハンドブック

  朝倉書店 2003
• Ultra-high-density Culture of a marine zooplankton Brachionus rotundiformis (Rotifer) using a membrane filtration

  Proceedings of current biotechnology and Bioengineering(XI) 2002
• ヘテロ乳酸発酵代謝マップ。醗酵ハンドブック

  共立出版 2001
• ポリヒドロキシ酪酸発酵。醗酵ハンドブック

  共立出版 2001
• Ultrahigh-density Culture System of the Rotifer, Brachionus rotundiformis. 3rd International Fish&Shellfish Larviculture Symposium “Larvi 2001”.

  Proceedings 2001
• Microbial production of biodegradable plastics from carbon dioxide and agricultural waste material.

  ACS. Symposium Series : 666, Fuels and Chemicals from Biomass. 1997
• Ferementative production of L-lactate from xylose.

  Development in Food Engineering, Proceedings of the 6th International Congress on Engineering and Food 1994
• Production of biopolymer for biodegradable plastic from carbon dioxide by using new fermentation system for gaseous substrate.

  The proceeding for global environment and energy issues, Fukuoka International Symposium 1990 1990

Conference Activities & Talks

• 透明な生分解性プラスチックを合成する水素細菌の分子育種

  板倉真優; 宮原 しろ沙; 田中賢二; 田口精一; 松崎弘美

  第60回化学関連支部合同九州大会  2023/07
• 乳酸ベースポリマーを生合成する Cupriavidus necator の分子育種

  板倉 真優; 宮原 しろ沙; 岡本 沙樹; 田中 賢二; 田口 精一; 松崎 弘美

  日本農芸化学会2023年度大会  2023/03
• Cupriavidus necatorを宿主とした乳酸ベースポリマーの生合成

  板倉真優; 宮原しろ沙; 岡本沙樹; 田中賢二; 田口精一; 松崎弘美

  第74回日本生物工学会大会  2022/10
• 乳酸ベースバイオプラスチックを合成するCupriavidus necatorの分子育種

  板倉真優; 岡本沙樹; 田中賢二; 田口精一; 松崎弘美

  第59回化学関連支部合同九州大会  2022/07
• 組換えCupriavidus necator による乳酸ベースバイプラスチックの生合成

  板倉 真優; 岡本 沙樹; 河原 あい; 田中 賢二; 田口 精一; 松崎 弘美

  日本農芸化学会2022年度大会  2022/03
• 組換えCupriavidus necatorによる高性能バイオプラスチックの生合成

  岡本沙樹; 板倉真優; 久永理央; 西愛美; 西上明花; 河原あい; 田中賢二; 田口精一; 松崎弘美

  第73回日本生物工学会大会  2021/10
• 新奇な光半導体の光電変換反応による非接触殺菌に関する研究

  田中賢二; 岩下真子; 米元舞; 伊東謙吾

  第73回日本生物工学会大会  2021/10
• CO2を原料とした発酵生産 ～ 水素酸化細菌によるグリーンプラスチック生産を例として  [Invited]

  田中賢二

  新化学技術推進協会ライフサイエンス技術部会  2021/09
• 組換え微生物を用いた高性能バイオプラスチックの生合成  [Not invited]

  岡本沙樹; 久永理央; 西愛美; 西上明花; 河原あい; 田中賢二; 田口精一; 松崎弘美

  第58回化学関連支部合同九州大会  2021/07
• ABC光半導体による非接触殺菌の可能性に関する検証  [Not invited]

  岩下真子; 米元舞; 伊東謙吾; 田中賢二

  第58回化学関連支部合同九州大会  2021/07
• 水素酸化細菌による生分解性プラスチックPHBHのCO2からの生合成  [Not invited]

  吉田和正; 折田和泉; 福居俊昭; 田中賢二

  第58回化学関連支部合同九州大会  2021/07
• 組換え微生物による高性能バイオポリエステルの生合成  [Not invited]

  岡本 沙樹; 久永 理央; 西 愛美; 西上 明花; 後藤 早希; 河原 あい; 田中 賢二; 田口 精一; 松崎 弘美

  日本農芸化学会2021年度大会  2021/03
• プラスチックを微生物からつくる究極の天然素材プラスチックの実用化をめざして  [Invited]

  ニュートン別冊『近畿大学 大解剖 vol.2』ニュートンプレス p.109  2021
• 新規乳酸ベースバイオポリマーの生合成における培養条件の検討  [Not invited]

  岡本 沙樹; 西上 明花; 後藤 早希; 松本 謙一郎; 阿部 英喜; 田口 精一; 田中 賢二; 松崎 弘美

  日本農芸化学会2020年度  2020/03
• 平板状銀ナノ粒子を用いた塗料状光半導体による殺菌と光照射の効果  [Not invited]

  田中 賢二; 宮本 樹里; 伊東 謙吾

  日本農芸化学会2020年度大会  2020/03
• Antibacterial activity of new ABC semiconductor and the effect of light irradiation  [Not invited]

  J. Miyamoto; K. Ito; K.Tanaka

  Materials Research Meeting 2019
• C.necator組換え株の化学合成独立栄養条件下での回分培養による3-ヒドロキシ酪酸と3-ヒドロキシヘキサン酸の共重合ポリエステルのCO2からの生合成  [Not invited]

  鈴木尚幸; 宮本 樹里; 折田和泉; 福居俊昭; 田中賢二

  第71回日本生物工学会大会  2019/09
• 化学合成独立栄養条件下における二酸化炭素からの共重合タイプの生分解性プラスチック生産新奇な塗料状光半導体の遮光後放電による殺菌の可能性  [Not invited]

  鈴木尚幸; 宮本樹里; 折田和泉; 福居俊昭; 田中賢二

  第56回化学関連支部合同九州大会  2019/07
• 新奇な塗料状光半導体の遮光後放電による殺菌の可能性  [Not invited]

  宮本樹里; 鈴木尚幸; 伊東謙吾; 田中賢二

  第56回化学関連支部合同九州大会  2019/07
• ABC光半導体の暗所での抗菌活性は遮光前の可視光照射によって増強される  [Not invited]

  小林加奈子; 辻塚 誠一郎; 宮本 樹里; 鈴木尚幸; 伊東 謙吾; 田中 賢二

  第28回日本MRS学会年次大会  2018/12
• 可視光および近赤外光照射下におけるABC光半導体の細菌に対する抗菌効果  [Not invited]

  宮本 樹里; 辻塚 誠一郎; 鈴木尚幸; 小林加奈子; 伊東 謙吾; 田中 賢二

  第28回日本MRS学会年次大会  2018/12
• 可視光および近赤外光照射下におけるABC光半導体のカビに対する抗菌効果  [Not invited]

  辻塚 誠一郎; 宮本 樹里; 鈴木尚幸; 小林加奈子; 伊東 謙吾; 田中 賢二

  第28回日本MRS学会年次大会  2018/12
• Biosynthesis of biodegradable lactate-based polymers with novel monomer compositions from glucose as the sole carbon source. T-2  [Not invited]

  Saki Goto; Ayaka Hokamura; Seiichi Taguchi; Ken'ichiro Matsumoto; Hideki Abe; Kenji Tanaka; Hiromi Matsusaki

  The 12th SPSJ International Polymer Conference (IPC2018)  2018/12
• 組換え微生物による脂肪酸合成経路を介したポリヒドロキシアルカン酸の生合成  [Not invited]

  西上 明花; 森 恵美; 後藤 早希; 阿部 英喜; 田中 賢二; 松崎 弘美

  平成30年度日本農芸化学会西日本支部大会  2018/09
• 新奇な塗料型光半導体の抗菌作用と光照射の効果  [Not invited]

  本 樹里; 辻塚 誠一郎; 田中 賢二

  近畿大学サイエンスネットワーク2018 第８回院生サミット  2018/09
• 遺伝子組換え微生物によるCO2からの共重合ポリエステルの生合成  [Not invited]

  鈴木 尚幸; 田中 賢二

  近畿大学サイエンスネットワーク2018 第８回院生サミット  2018/09
• ABC 光半導体の暗所での殺菌作用における遮光後放電による影響の可能性  [Not invited]

  辻塚 誠一郎; 宮本 樹里; 俗多 秀美; 伊東 謙吾; 田中 賢二

  第70回日本生物工学会大会  2018/09
• 遺伝子組換え水素酸化細菌によるCO2 からの共重合ポリエステルの生合成とC6 ユニットモル分率の変化  [Not invited]

  鈴木 尚幸; 森 瞬也; 折田 和泉; 福居 俊昭; 田中 賢二

  第70回日本生物工学会大会  2018/09
• Improvement of monomer composition in poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesized from CO2 by genetically engineered hydrogen-oxidizing bacterium  [Not invited]

  Tanaka K; Suzuki N; Mori S; Izumi O; Fukui T

  World Congress on Biopolymers and Bioplastics (Biopolymer 2018)  2018/08
• 遺伝子組換え水素酸化細菌によるCO2からの生分解性プラスチックの生合成  [Not invited]

  鈴木尚幸; 宮本樹里; 辻塚誠一郎; 折田和泉; 福居俊昭; 田中賢二

  第55回化学関連支部合同九州大会  2018/06
• 量産型ABC光半導体の細菌に対する抗菌効果  [Not invited]

  宮本樹里; 辻塚誠一郎; 鈴木尚幸; 伊東謙吾; 田中賢二

  第55回化学関連支部合同九州大会  2018/06
• 量産型ABC光半導体のカビに対する抗菌効果  [Not invited]

  辻塚誠一郎; 宮本樹里; 鈴木尚幸; 伊東謙吾; 田中賢二

  第55回化学関連支部合同九州大会  2018/06
• A Novel Efficient Antibacterial Action of ABC semiconductors  [Not invited]

  Kengo Ito; Tohru Nishibe; Masakazu Kawahara; Seiichiro Tsujitsuka; Kenji Tanaka

  10th International Symposium on Organic Molecular Electronics (ISOME 2018)  2018/05
• 透明性を有する新規乳酸ベースポリマーの生合成  [Not invited]

  後藤 早希; 龍野 菜々美; 松本 謙一郎; 阿部 英喜; 田口 精一; 田中 賢二; 松崎 弘美

  日本農芸化学会2018年度大会  2018/03
• 組換え微生物による実用的なポリヒドロキシアルカン酸の生合成  [Not invited]

  森 恵美; 西上 明花; 後藤 早希; 阿部 英喜; 田中 賢二; 松崎 弘美

  日本農芸化学会2018年度大会  2018/03
• Antimicrobial effect of a novel photosemiconductor composed of Ag nanoplate, boron and clay, and its increase by visible light irradiation  [Not invited]

  Kenji Tanaka; Kojiro Yamamoto; Seiichiro Tsujituka; Kengo Itou

  BIT’s 4th Annual World Congress of Smart Materials-2018 (WCSM-2018)  2018/03
• 糖を炭素源とした新規の乳酸ベースポリマーの微生物合成  [Not invited]

  後藤早希; 龍野菜々美; 松本謙一郎; 阿部英喜; 田口精一; 田中賢二; 松崎弘美

  第24回日本生物工学会九州支部大会  2017/12
• 新規な塗料状光半導体材料の暗所と可視光及び近赤外光照射下での抗菌特性に関する研究  [Not invited]

  辻塚 誠一郎; 山本 幸次郎; 伊東 謙吾; 田中 賢二

  第69回日本生物工学会大会  2017/09
• 可視光・近赤外光応答性光半導体加工フィルムの抗菌特性に関する研究  [Not invited]

  山本 幸次郎; 辻塚 誠一郎; 伊東 謙吾; 田中 賢二

  第69回日本生物工学会大会  2017/09
• CO2 からの共重合ポリエステル P(3HB-co-3HHx)生合成と PhaJAc 遺伝子の導入効果  [Not invited]

  森 舜也; 丸尾 浩希; 折田 和泉; 福居 俊昭; 田中 賢二

  第69回日本生物工学会大会  2017/09
• 組換え微生物による生分解性共重合ポリエステルの生合成  [Not invited]

  森 恵美; 倉富 優季; 後藤 早希; 脇田 和; 外村 彩夏; 阿部 英喜; 田中 賢二

  第69回日本生物工学会大会  2017/09
• 組換え大腸菌による新規モノマー組成からなる乳酸ベースポリマーの生合成  [Not invited]

  後藤 早希; 外村 彩夏; 田口 精一; 松本 謙一郎; 阿部 英喜; 田中 賢二; 松崎 弘美

  第69回日本生物工学会大会  2017/09
• BIOSYNTHESIS OF POLY (3-HYDROXYBUTYRATE -CO- 3-HYDROXYHEXANOATE) FROM FRUCTOSE AND CO2 BY THE ENGINEERED RALSTONIA EUTROPHA  [Not invited]

  Kenji Tanaka; Shunya Mori; Kouki Maruo; Orita Izumi; Toshiaki Fukui

  Biomaterials International 2017, Fukuoka Japan  2017/08
• 新規の生分解性乳酸ベースポリマーの生合成  [Not invited]

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美

  第54回化学関連支部合同九州大会  2017/07
• BC光半導体の暗所と近赤外光照射下における抗菌活性に関する研究  [Not invited]

  山本 幸次郎; 辻塚 誠一郎; 伊東 謙吾; 田中 賢二

  第54回化学関連支部合同九州大会  2017/07
• 可視光応答型光半導体様化合物の暗所と光照射下における抗菌活性に関する研究  [Not invited]

  辻塚 誠一郎; 山本 幸次郎; 伊東 謙吾; 田中 賢二

  第54回化学関連支部合同九州大会  2017/07
• 遺伝子組換え株によるCO2からの共重合グリーンプラスチック生合成におけるモノマー組成の改善  [Not invited]

  森 舜也; 丸尾 浩希; 折田 和泉; 福居 俊昭; 田中 賢二

  第54回化学関連支部合同九州大会  2017/07
• Effect of fatty acid in culture medium on growth of acid tolerant lactic acid bacterium, Lactobacillus acetotolerans strain HT  [Not invited]

  Kenji Tanaka; Kiko Sakai; Ryo Sawada; Saki Goto; Hiromi Matsusaki

  The 6th International Congress o Natural Sciences and Engineering (2017 ICNSE), Kitakyuhu Japan  2017/06
• 組換え大腸菌による新規乳酸ベースポリマーの生合成  [Not invited]

  後藤早希; 留野菜月; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美

  日本農芸化学会2017年度大会  2017/03
• 組換えRalstonia eutrophaによる糖からのP(3HB-co-3HA)の生合成  [Not invited]

  倉富優季; 脇田和; 後藤早希; 森恵美; 外村彩夏; 田中賢二; 松崎弘美

  日本農芸化学会2017年度大会  2017/03
• Ralstonia eutrophaを宿主とした糖からのP(3HB-co-3HA)の生合成  [Not invited]

  森恵美; 倉富優季; 後藤早希; 脇田和; 外村彩夏; 田中賢二; 松崎弘美

  第23回日本生物工学会九州支部大会  2016/12
• 糖から共重合ポリエステルを合成する大腸菌の分子育種  [Not invited]

  倉富優季; 森恵美; 後藤早希; 脇田和; 外村彩夏; 田中賢二; 松崎弘美

  第23回日本生物工学会九州支部大会  2016/12
• Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子のクローニングと新規乳酸ベースポリマーの生合成  [Not invited]

  後藤早希; 留野菜月; 外村彩夏; 松本謙一; 田口精一; 阿部英喜; 田中賢二; 松崎弘美

  第23回日本生物工学会九州支部大会  2016/12
• 銀ナノ粒子・ボロン樹脂・クレイからなる新規光触媒の近赤外光照射下における殺菌効果  [Not invited]

  山本 幸次郎; 深野木 伸太; 宮﨑 愛; 伊東 謙吾; 田中 賢二

  第68回日本生物工学会大会  2016/09
• 銀ナノ粒子・ボロン樹脂・クレイからなる新規光触媒の暗所と可視光照射下における病原細菌殺菌効果  [Not invited]

  宮﨑 愛; 深野木 伸太; 山本 幸次郎; 伊東 謙吾; 田中 賢二

  第68回日本生物工学会大会  2016/09
• 銀ナノ粒子・ボロン樹脂・クレイからなる新規光触媒の暗所と可視光照射下におけるカビ胞子に対する殺菌効果  [Not invited]

  深野木 伸太; 宮﨑 愛; 山本 幸次郎; 伊東 謙吾; 田中 賢二

  第68回日本生物工学会大会  2016/09
• 3HHx を高含有率で含む共重合ポリエステルの遺伝子組換え水素細菌による CO2 からの生合成  [Not invited]

  森 舜也; 丸尾 浩希; 折田 和泉; 福居 俊昭; 田中 賢二

  第68回日本生物工学会大会  2016/09
• 組換え大腸菌による乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成  [Not invited]

  後藤 早希; 中上 美歩; 外村 彩夏; 田口 精一; 松本 謙一郎; 田中 賢二; 松崎 弘美

  日本農芸化学会2016年度西日本支部大会  2016/09
• 糖を唯一の炭素源として共重合ポリエステルを合成する細菌の分子育種  [Not invited]

  倉富優季; 脇田和; 後藤早希; 外村彩夏; 田中賢二; 松崎弘美

  日本農芸化学会2016年度西日本支部大会  2016/09
• Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C6-monomer composition from CO2 by recombinant of Ralstonia eutropha  [Not invited]

  Kenji Tanaka; Shunya Mori; Kouki Maruo; Orita Izumi; Toshiaki Fukui

  3rd International Conference and Exhibition on Biopolymers & Bioplastics  2016/09
• Growth characteristics of newly isolated xylose-assimilating bacterium and accumulation of green plastic, polyhydroxyalkanoate in the genetic engineered strain  [Not invited]

  Kenji Tanaka; Shunya Mori; Kastuki Matsumoto; Kojiro Yamamoto; Izumi Wakida; Saki Goto; Hiromi Mastusaki

  International Symposium on Life Science & Biological Engineering 2016 (I SLSBE2016)  2016/08
• 乳酸ユニットを含む新規モノマー組成からなる生分解性プラスチックの生合成  [Not invited]

  後藤 早希; 外村 彩夏; 田口 精一; 松本 謙一郎; 岡部英喜; 田中 賢二; 松崎 弘美

  第53回化学関連支部合同九州大会  2016/07
• 水素細菌の遺伝子組換え株によるC6モノマーを高モル分率で含む共重合ポリエステルのCO2からの生合成  [Not invited]

  森 舜也; 丸尾 浩希; 折田 和泉; 福居 俊昭; 田中 賢二

  第53回化学関連支部合同九州大会  2016/07
• 平板状銀ナノ粒子を用いた近赤外光応答型光触媒の細菌に対する殺菌効果  [Not invited]

  山本幸次郎; 深野木伸太; 宮﨑 愛; 伊東謙吾; 田中 賢二

  第53回化学関連支部合同九州大会  2016/07
• 平板状銀ナノ粒子を用いた可視光応答型光触媒のカビ胞子に対する殺菌効果  [Not invited]

  第53回化学関連支部合同九州大会  2016/07
• 平板状銀ナノ粒子を用いた可視光応答型光触媒の病原細菌に対する殺菌効果  [Not invited]

  宮崎 愛; 深野木 伸太; 山本 幸次郎; 伊東 謙吾; 田中 賢二

  第53回化学関連支部合同九州大会  2016/07
• Autotrophic Growth of Paracoccus Denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO2  [Not invited]

  Kenji Tanaka; Syunya, Mori; May Hirata; Hiromi Matsusaki

  The Global Conference on Life Science and Biological Engineering 2016(GLSBE 2016)  2016/03
• 組換え大腸菌による乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成  [Not invited]

  後藤早希; 中上美歩; 外村彩夏; 田口精一; 松本謙一郎; 田中賢二; 松崎弘美

  日本農芸化学会2016年度大会  2016/03
• 組換えRalstonia eutrophaによる糖からのP(3HB-co-3HA)共重合ポリエステルの生合成  [Not invited]

  倉富優季; 脇田和; 外村彩夏; 田中賢二; 松崎弘美

  日本農芸化学会2016年度大会  2016/03
• Inhibitory effect to pathogenic bacteria of visible light responsive type nanosilver photocatalyst  [Not invited]

  Shintaro Tajiri; Megumi Miyazaki; Kenji Tanaka; Kengo Ito

  2015 International Chemical Congress of Pacific Basin Societies, Section ORG  2015/12
• 組換え大腸菌による新規モノマー組成からなる共重合ポリエステルの生合成  [Not invited]

  中上美歩; 後藤早希; 西村綾乃; 外村彩夏; 田口精一; 松本謙一郎; 田中賢二; 松崎弘美

  第22回日本生物工学会九州支部大会  2015/12
• 共重合ポリエステルを合成するRalstonia eutrophaの分子育種  [Not invited]

  倉富優季; 脇田和; 外村彩夏; 田中賢二; 福居俊昭; 柘植丈治; 松崎弘美

  第22回日本生物工学会九州支部大会  2015/12
• 乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成  [Not invited]

  後藤 早希; 外村 彩夏; 西村 綾乃; 田口 精一; 松本 謙一郎; 田中 賢二; 松崎 弘美

  第67回日本生物工学会大会  2015/10
• 遺伝子組換え菌による糖からのP(3HB-co-3HA)共重合ポリエステルの生合成に関する研究  [Not invited]

  脇田 和; 外村 彩夏; 柘植 丈治; 田中 賢二; 松崎 弘美

  第67回日本生物工学会大会  2015/10
• 可視光応答型銀ナノ粒子光触媒のカビに対する抗菌効果  [Not invited]

  宮﨑 愛; 深野木 伸太; 田尻 晋太郎; 伊東 謙吾; 田中 賢二

  第67回日本生物工学会大会  2015/10
• 可視光応答型銀ナノ粒子光触媒の病原細菌に対する抗菌効果  [Not invited]

  宮﨑 愛; 深野木 伸太; 田尻 晋太郎; 伊東 謙吾; 田中 賢二

  第67回日本生物工学会大会  2015/10
• Enterobacter sp.TF 株の遺伝子組換え株によるキシロースからの生分解性ポリエステル蓄積率改善に関する研究  [Not invited]

  松本 活樹; 藏冨 友博; 外村 彩夏; 松崎 弘美; 田中 賢二

  第67回日本生物工学会大会  2015/10
• 銀ナノ粒子を用いた可視光応答型光触媒の抗菌活性~フィルム密着法による評価~  [Not invited]

  田尻 晋太郎; 宮﨑 愛; 深野木 伸太; 伊東 謙吾; 田中 賢二

  第67回日本生物工学会大会  2015/10
• 水素酸化細菌によるCO2 からのポリエステル合成における3-ヒドロキシアシルCoA リガーゼ遺伝子の導入効果  [Not invited]

  藏冨 友博; 松本 活樹; 外村 彩夏; 松崎 弘美; 田中 賢二

  第67回日本生物工学会大会  2015/10
• 耐酸性乳酸菌の増殖に対する培地脂肪酸の影響  [Not invited]

  沢田 僚; 深野木 伸太; 田尻 晋太郎; 田中 賢二

  第67回日本生物工学会大会  2015/10
• Acid-tolerant lactic acid bacterium isolated from rice vinegar  [Not invited]

  Kenji Tanaka; Shintaro Tajiri; Ryo Sawada; Yoshihiro Kawamoto; Teruaki Matsubara; Munehiro Hosihino; Hiromi Matsusaki

  The Annual Conference on Life Sciences and Engineering (2015 ACLSE)  2015/08
• The Effect of Visible Light with Different Wavelength and Intensities on Population Growth of Marine Zooplankton, Brachionus Plicatilis  [Not invited]

  Kenji Tanaka; Tomohito Amai; Tomohiro Kuratomi; Katsuki Matsumoto; Shintaro Tajiri

  The 2nd International Biotechnology, Chemical Engineering and Life Science Conference (IBCELC 2015)  2015/07
• CO2から新奇な共重合ポリエステルを合成する遺伝子組換え微生物の創製  [Not invited]

  藏冨 友博; 松本 活樹; 田中 賢二

  第５回近畿大学サイエンスネットワーク院生サミット  2015/07
• 銀ナノ粒子を用いた可視光応答型光触媒の抗菌効果に関する研究  [Not invited]

  宮﨑 愛; 田尻 晋太郎; 深野木 伸太; 田中 賢二

  第５回近畿大学サイエンスネットワーク 院生サミット  2015/07
• 銀ナノ粒子光触媒の病原細菌に対する抗菌活性と可視光照射の効果  [Not invited]

  宮崎愛; 田尻晋太郎; 深野木伸太; 伊東謙吾; 田中賢二

  第52回化学関連支部合同九州大会  2015/06
• 食酢から分離された乳酸菌の増殖に対する脂肪酸の影響  [Not invited]

  沢田僚; 深野木伸太; 田尻晋太郎; 田中 賢二

  第52回化学関連支部合同九州大会  2015/06
• 可視光応答型銀ナノ粒子光触媒のフィルム密着法による抗菌力評価  [Not invited]

  田尻晋太郎; 宮崎愛; 深野木伸太; 伊東謙吾; 田中賢二

  第52回化学関連支部合同九州大会  2015/06
• キシロース資化性エンテロバクター属組換え株のPHA蓄積率に重合酵素が及ぼす影響  [Not invited]

  松本活樹; 藏冨友博; 外村彩夏; 松崎弘美; 田中 賢二

  第52回化学関連支部合同九州大会  2015/06
• 3HA-CoAリガーゼを導入した水素酸化細菌によるCO2からのPHA合成. 藏冨友博  [Not invited]

  藏冨友博; 松本活樹; 外村彩夏; 松崎弘美; 田中 賢二

  第52回化学関連支部合同九州大会  2015/06
• 組換え大腸菌による糖からの生分解性共重合ポリエステルの生合成.  [Not invited]

  外村 彩夏; 西村 綾乃; 後藤 早希; 脇田 和; 田口 精一; 松本 謙一郎; 柘植 丈治; 田中 賢二; 松崎 弘美

  日本農芸化学会2015年度大会  2015/03
• 耐酸性乳酸菌Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子のクローニングと生分解性共重合ポリエステルの生合成  [Not invited]

  後藤 早希; 外村 彩夏; 田口 精一; 松本 謙一郎; 田中 賢二; 松崎 弘美

  日本農芸化学会2015年度大会  2015/03
• 脂肪酸合成経路を介した糖からの共重合ポリエステルの生合成  [Not invited]

  外村 彩夏; 西村 綾乃; 柘植 丈治; 福居 俊昭; 田中 賢二; 松崎 弘美

  日本農芸化学会2015年度大会  2015/03
• 銀ナノ粒子を用いた可視光応答型光半導体の病原細菌に対する抗菌効果  [Not invited]

  田尻 晋太郎; 宮﨑 愛; 伊東 謙吾; 田中 賢二

  日本農芸化学会2015年度大会  2015/03
• 乳酸ユニットを含む生分解性共重合ポリエステルの生合成  [Not invited]

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 田中賢二; 松崎弘美

  第21回日本生物工学会九州支部大会  2014/12
• 糖および炭酸ガスからの共重合ポリエステル生合成に関する研究  [Not invited]

  脇田 和; 外村彩夏; 西村綾乃; 田中賢二; 福居俊昭; 柘植丈治; 松崎弘美

  第21回日本生物工学会九州支部大会  2014/12
• 銀ナノ粒子を用いた新規な可視光応答型光触媒の抗菌効果  [Not invited]

  田尻 晋太郎; 宮﨑 愛; 伊東 謙吾; 田中 賢二

  第66回日本生物工学会大会  2014/09
• Lactobacillus acetotolerans HT の乳酸脱水素酵素遺伝子を利用した乳酸ユニットを含む生分解性プラスチックの生合成  [Not invited]

  後藤 早希; 佐藤 美咲; 田中 賢二; 松本 謙一郎; 田口 精一; 松崎 弘美

  第66回日本生物工学会大会  2014/09
• Ralstonia eutrophaを宿主とした糖および炭酸ガスからのポリヒドロキシアルカン酸生合成  [Not invited]

  脇田 和; 外村 彩夏; 岩崎 美佳; 田中 賢二; 福居 俊昭; 柘植 丈治; 松崎 弘美

  第66回日本生物工学会大会  2014/09
• CO2から共重合PHA を合成する水素酸化細菌組換え株の育種  [Not invited]

  藏冨 友博; 平尾 崚; 松崎 弘美; 外村 彩夏; 田中 賢二

  第66回日本生物工学会大会  2014/09
• キシロース資化性Enterobacter属細菌の遺伝子組換えによるPHA 生合成  [Not invited]

  松本 活樹; 南部 友香; 松崎 弘美; 田中 賢二

  第66回日本生物工学会大会  2014/09
• キシロース資化性細菌の分離と生分解性ポリエステル合成遺伝子の導入  [Not invited]

  松本活樹; 南部友香; 松崎弘美; 田中賢二

  第51回化学関連支部合同九州大会  2014/06
• 水素酸化細菌の遺伝子組換え株における共重合ポリエステル合成の問題点  [Not invited]

  藏冨友博; 平尾崚; 松崎弘美; 田中賢二

  第51回化学関連支部合同九州大会  2014/06
• 食酢から分離された乳酸菌の耐酸性と細胞膜脂肪酸組成の関連  [Not invited]

  田尻晋太郎; 沢田僚; 田中賢二

  第51回化学関連支部合同九州大会  2014/06
• 127. 脂肪酸合成経路を介した糖からの共重合ポリエステルの生合成  [Not invited]

  外村 彩夏; 岩崎 美佳; 村田 和歌子; 明智 聡; 福居 俊昭; 柘植 丈治; 田中 賢二; 松崎 弘美

  日本農芸化学会2014年度大会  2014/03
• Lactobacillus acetotolerans HTの乳酸脱⽔素酵素遺伝⼦のクローニングと乳酸ユニットを含む⽣分解性プラスチックの⽣合成  [Not invited]

  後藤早希; 佐藤美咲; 田中賢二; 松本謙一郎; 田口精一; 松崎弘美

  第20回日本生物工学会九州⽀部⼤会  2013/12
• 組換えRalstonia eutrophaにおける脂肪酸合成経路を介したポリヒドロキシアルカン酸の生合成  [Not invited]

  岩崎 美佳; 外村 彩夏; 村田 和歌子; 田中 賢二; 柘植 丈治; 松崎 弘美

  第65回日本生物工学会大会  2013/09
• 資源管理型漁業のための動物性初期餌料生物の培養生産の現状と光利用による生産効率化の可能性  [Not invited]

  田中 賢二

  日本農芸化学会関西，中四国，西日本支部2013年度合同広島大会  2013/09
• 光照射とシオミズツボワムシの増殖および栄養環境 シンポジウム「水産における光利用技術と基礎研究の動向」  [Not invited]

  田中 賢二

  平成25 年度日本水産学会春季大会  2013/03
• 脂肪酸合成経路を介したポリヒドロキシアルカン酸の⽣合成  [Not invited]

  坂本日名子; 外村彩夏; 岩崎美佳; 田中賢二; 松崎弘美; 熊本県大; 環境共生

  第19回日本生物工学会九州支部大会  2012/12
• 酢酸耐性乳酸菌Lactobacillus acetotolerans HT株のL型およびD型乳酸脱⽔素酵素遺伝⼦のクローニング  [Not invited]

  佐藤美咲; 元村あかね; 田中賢二; 松崎弘美

  第19回日本生物工学会九州支部大会  2012/12
• Biosynthesis of coplymer by recombinant Ralstonia eutropha through fatty acid synthesis pathway  [Not invited]

  岩崎 美佳; 外村 彩夏; 松崎 弘美; 田中 賢二; 柘植 丈治

  第64回日本生物工学会大会  2012/10  神戸市  第64回日本生物工学会大会
  
• Characterization of sulfate-reducing bacteria isolated from a microaerobic waste water treatment plant  [Not invited]

  福本 圭; 原田 奏也; 水野 康平; 田中 賢二

  第64回日本生物工学会大会  2012/10  神戸市  第64回日本生物工学会大会
  
• 伝統食品と発酵  [Not invited]

  田中 賢二

  飯塚市 食のセミナー講演会  2012/10  飯塚市  飯塚市 食のセミナー講演会
  
• 動物性海産餌料生物の増殖に光照射が及ぼす影響とビタミンB12産生細菌の関与  [Not invited]

  田中 賢二

  平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会 九州・沖縄支部合同大会合同大会  2012/09  鹿児島市  平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会 九州・沖縄支部合同大会合同大会
  
• DsrBGene-Based DGGE Analysis of the Inhibitory Effect of Nitrate on the Sulfate-reducing Bacterial Community Dynamics in a Laboratory-scale Wastewater Reactor  [Not invited]

  森下 由唯; 田中 賢二

  日本農芸化学会2012年度大会  2012/03  京都市  日本農芸化学会2012年度大会
  
• 光照射は海産種苗の初期餌料動物プランクトン、ワムシの増殖密度を増加させる  [Not invited]

  田中 賢二

  日本農芸化学会2012年度大会  2012/03  京都市  日本農芸化学会2012年度大会
  
• L型ワムシの増殖の比較  [Not invited]

  田中 賢二

  平成24年度日本水産学会春季大会  2012/03  平成24年度日本水産学会春季大会
  
• 鍾乳洞より分離した新種細菌 Jeongeupia sp. HS -3 の分類同定  [Not invited]

  田中 賢二; 北九州工業高等専門学校

  日本農芸化学会2012年度大会  2012/03  京都市  日本農芸化学会2012年度大会
  
• 栽培漁業用初期餌料動物プランクトンの光照射下での増殖促進とビタミンB12の関係  [Not invited]

  田中 賢二

  第49回化学関連支部合同九州大会  2012  北九州市  第49回化学関連支部合同九州大会
  
• 微好気排水より分離した高過酸化水素耐性硫酸還元菌の性状分析  [Not invited]

  田中 賢二; 北九州工業高等専門学校

  日本生物工学会第18回九州支部大会  2011/12  1  日本生物工学会第18回九州支部大会
  
• Ralstonia eutrophaにおける脂肪酸合成経路からの共重合ポリエステル生合成に関する検討  [Not invited]

  田中 賢二

  日本生物工学会第18回九州支部大会  2011/12  福岡市  日本生物工学会第18回九州支部大会
  
• 鍾乳洞より分離した新種細菌Jeongeupia sp. HS-3の分類同定  [Not invited]

  田中 賢二; 北九州工業高等専門学校

  日本生物工学会第18回九州支部大会  2011/12  福岡市  日本生物工学会第18回九州支部大会
  
• 辛子めんたいこ調味液より分離した細菌のGABA 生成能について  [Not invited]

  田中 賢二; 株)やまやコミュニケーションズ

  日本食品科学工学会  2011/09  仙台市  日本食品科学工学会
  
• 排水処理汚泥の細菌叢解析による硫化水素発生プロセスとその影響因子に関する研究  [Not invited]

  田中 賢二; 北九州工業高等専門学校; 九州電力; 総合研究所生物資源研究センター

  第63回日本生物工学会大会  2011/09  東京  第63回日本生物工学会大会
  
• 水産餌料動物プランクトン培養におけるLED光の増殖促進効果  [Not invited]

  田中 賢二

  第63回日本生物工学会大会  2011/09  東京  第63回日本生物工学会大会
  
• 共重合P H A 生合成遺伝子を導入したRalstonia eutropha組換え株の独立栄養条件下での菌体合成  [Not invited]

  田中 賢二

  平成23 年度日本農芸化学会西日本支部・中四国支部合同大会  2011/09  宮崎市  平成23 年度日本農芸化学会西日本支部・中四国支部合同大会
  
• 脂肪酸合成経路を介した組換えRalstonia eutrophaによる共重合ポリエステルの生合成  [Not invited]

  田中 賢二

  第63回日本生物工学会大会  2011/09  東京  第63回日本生物工学会大会
  
• 独立栄養条件下で増殖可能なParacoccus属細菌の探索に関する研究  [Not invited]

  田中 賢二

  第48回化学関連支部合同九州大会  2011/07  北九州市  第48回化学関連支部合同九州大会
  
• 水素酸化細菫の独立栄養条件下での増殖に対する壼素源の影響  [Not invited]

  田中 賢二

  第48回化学関連支部合同九州大会  2011/07  北九州市  第48回化学関連支部合同九州大会
  
• テラヘルツ分光法による微生物菌体内生産物の検出  [Not invited]

  田中 賢二

  第48回化学関連支部合同九州大会  2011/07  北九州市  第48回化学関連支部合同九州大会
  
• 脂肪酸合成経路を介した組換えRalstonia eutrophaによるポリヒドロキシアルカン酸合成  [Not invited]

  田中 賢二

  日本農芸化学会2011年度大会  2011/03  京都市  日本農芸化学会2011年度大会
  
• CO2からの共重合体PHA合成を目的とした水素酸化細菌の育種と独立栄養条件下での培養特性  [Not invited]

  田中 賢二

  日本農芸化学会2011年度大会  2011/03  京都市  日本農芸化学会2011年度大会
  
• 組換えRalstonia eutropha による糖および炭酸ガスからの共重合ポリエステルの生合成  [Not invited]

  田中 賢二

  日本生物工学会大会2010年度大会  2010/10  宮崎市  日本生物工学会大会2010年度大会
  
• たらこにおける細菌の亜硝酸根生成とその抑制に関する研究  [Not invited]

  田中 賢二

  日本食品科学工学会2010年度大会  2010/09  日本食品科学工学会2010年度大会
  
• O2から共重合ポリエステルを合成するためのRalstonia eutrophaの組換え株の作成と独立栄養条件下での増殖  [Not invited]

  田中 賢二

  第47回化学関連支部合同九州大会  2010/07  北九州市  第47回化学関連支部合同九州大会
  
• Paracoccus denitrificansのＣＯ2とＨ2を基質とする菌体増殖と物質生産  [Not invited]

  田中 賢二

  第47回化学関連支部合同九州大会  2010/07  北九州市  第47回化学関連支部合同九州大会
  
• 排水処理汚泥における硫化水素発生とその影響因子の細菌叢解析による研究  [Not invited]

  田中 賢二

  日本農芸化学会2010年度大会  2010/03  東京  日本農芸化学会2010年度大会
  
• 共重合ポリエステルの生合成における微生物育種に関する研究  [Not invited]

  田中 賢二

  第16回日本生物工学会九州支部大会  2009/12  飯塚市  第16回日本生物工学会九州支部大会
  
• 排水処理汚泥における硫化水素発生の可能性とその影響因子に関する研究  [Not invited]

  田中 賢二

  第16回日本生物工学会九州支部大会  2009/12  第16回日本生物工学会九州支部大会
  
• 排水処理汚泥の細菌叢解析による硫化水素発生プロセスとその影響因子に関する研究  [Not invited]

  田中 賢二

  第16回日本生物工学会九州支部大会  2009/12  飯塚市  第16回日本生物工学会九州支部大会
  
• 水素酸化細菌R.eutrophaとA.latusの独立栄養条件下での生育特性比較  [Not invited]

  田中 賢二

  第16回日本生物工学会九州支部大会  2009/11  飯塚市  第16回日本生物工学会九州支部大会
  
• 初期餌料生物Brachionus plicatilis生産の高密度化技術の開発  [Not invited]

  田中 賢二

  近畿大学サイエンスネットワーク・院生サミット  2009/11  東大阪市  近畿大学サイエンスネットワーク・院生サミット
  
• 近畿大学サイエンスネットワーク・院生サミット  [Not invited]

  田中 賢二

  2009/11
• 離島発電所で発生する廃油分解微生物の探索  [Not invited]

  田中 賢二

  2009年度日本農芸化学会関西・中四国･西日本支部、日本栄養･食糧学会九州･沖縄支部および日本食品化学工学会西日本支部合同大会  2009/10  沖縄  2009年度日本農芸化学会関西・中四国･西日本支部、日本栄養･食糧学会九州･沖縄支部および日本食品化学工学会西日本支部合同大会
  
• たらこから分離した細菌の亜硝酸根生成能について  [Not invited]

  田中 賢二

  日本食品科学工学会第56回大会  2009/09  名古屋市  日本食品科学工学会第56回大会
  
• Ideonella sp.Ongaの旺盛なキシロース資化性と有用物質生産の可能性  [Not invited]

  田中 賢二

  第46回化学関連支部合同九州大会  2009/07  第46回化学関連支部合同九州大会
  
• 水素酸化細菌Alcaligenes latusの独立栄養条件下における増殖とPHB蓄積  [Not invited]

  田中 賢二

  第46回化学関連支部合同九州大会  2009/07  北九州市  第46回化学関連支部合同九州大会
  
• もろみ酢から分離された乳酸菌の高濃度乳酸発酵  [Not invited]

  田中 賢二

  第45回化学関連支部合同九州大会  2008/07  北九州市  第45回化学関連支部合同九州大会
  
• 高密度培養における海産餌料プランクトンS型ワムシとL型ワムシの増殖特性の違い  [Not invited]

  田中 賢二

  第45回化学関連支部合同九州大会  2008/07  北九州市  第45回化学関連支部合同九州大会
  
• 松原輝明、緒方祐夏、星野宗広、上野裕、田中賢二  [Not invited]

  田中 賢二

  第42回化学関連支部合同九州大会  2005/07  北九州市  第42回化学関連支部合同九州大会
  
• ＰＨＢ産生化学合成独立栄養細菌Idenonella sp.Ongaは多様な糖資化性を持つ  [Not invited]

  田中 賢二

  第42回化学関連支部合同九州大会  2005/07  北九州市  第42回化学関連支部合同九州大会
  
• 水産初期餌料動物プランクトンＬ型ワムシの高密度培養に出現したワムシに特異的な有害菌について  [Not invited]

  田中 賢二

  第42回化学関連支部合同九州大会  2005/07  北九州市  第42回化学関連支部合同九州大会
  
• 新規に発見された高酸度酢酸発酵優良菌の発酵特性  [Not invited]

  田中 賢二

  第42回化学関連支部合同九州大会  2005/07  北九州市  第42回化学関連支部合同九州大会
  
• 一酸化炭素に強い耐性をもつ水素酸化細菌の諸性質と増殖特性  [Not invited]

  田中 賢二

  日本農芸化学会2005年度大会  2005/03  札幌市  日本農芸化学会2005年度大会
  
• 水産初期餌料動物プランクトンから分離された細菌群のビタミンＢ12生成能について  [Not invited]

  田中 賢二

  第42回化学関連支部合同九州大会  2005  北九州市  第42回化学関連支部合同九州大会
  
• 水産餌料用動物プランクトン、ワムシの増殖因子獲得における海洋細菌の関与  [Not invited]

  田中 賢二

  平成１６年度日本生物工学大会  2004/09  名古屋市  平成１６年度日本生物工学大会
  
• マイナス空気イオンの抗菌効果に関する研究  [Not invited]

  田中 賢二

  平成１６年度日本生物工学大会  2004/09  名古屋市  平成１６年度日本生物工学大会
  
• これだけは知っておきたい微生物 －食と健康のキーワード－  [Not invited]

  田中 賢二

  シニアサマーカレッジ「高齢社会と環境」  2004/09  北九州市  シニアサマーカレッジ「高齢社会と環境」
  
• 水産餌料用動物プランクトン，Ｌ型ワムシの大量培養法に関する研究  [Not invited]

  田中 賢二

  第41回化学関連支部合同九州大会  2004/07  北九州市  第41回化学関連支部合同九州大会
  
• 遠賀川から分離した化学合成独立栄養細菌の分子生物学的手法による分類同定  [Not invited]

  田中 賢二; 浅野徳太郎

  第41回化学関連支部合同九州大会  2004/07  北九州市  第41回化学関連支部合同九州大会
  
• 福岡県田川郡の食酢メーカーで受け継がれてきた高酸度酢酸発酵用種酢の菌学的解析  [Not invited]

  田中 賢二

  第41回化学関連支部合同九州大会  2004/07  北九州市  第41回化学関連支部合同九州大会
  
• 水産餌料用動物プランクトン，Ｌ型ワムシに対する塩化コバルト添加の増殖促進効果  [Not invited]

  田中 賢二

  第41回化学関連支部合同九州大会  2004/07  北九州市  第41回化学関連支部合同九州大会
  
• マイナス空気イオン発生器の抗菌への利用 -抗菌活性の評価-  [Not invited]

  田中 賢二

  第41回化学関連支部合同九州大会  2004/07  第41回化学関連支部合同九州大会
  
• なぜコバルト塩添加や光照射でワムシの増殖が向上するのか － ビタミンB12産生からの説明  [Not invited]

  田中 賢二

  2004年度 日本水産学会大会  2004/03  2004年度 日本水産学会大会
  
• 水産餌料用動物プランクトン、ワムシの換水培養において換水率が細菌相に及ぼす影響  [Not invited]

  田中 賢二; 倉本将毅; 吉村研治

  平成15 年度日本生物工学大会（熊本）  2003/09  平成15 年度日本生物工学大会（熊本）
   

  水産餌料用動物プランクトンであるワムシの換水培養において換水率によってワムシ体内や培養水中の細菌数がどのように増減するか調べた。また、各換水率での優占菌種を簡易同定法や16S rRNA 遺伝子相同性によって道程することにより、どの換水率がワムシの衛生状態に最も良好か明らかにした。
• 水素酸化細菌Ideonella sp. の化学合成独立栄養条件下での増殖とPHB 蓄積  [Not invited]

  田中 賢二; 宮田典久

  平成15 年度日本生物工学大会（熊本）  2003/09  平成15 年度日本生物工学大会（熊本）
   

  当研究室で分離した水素酸化細菌Ideonella sp. の独立栄養条件下での増殖特性、生分解性プラスチックであるPHB の生産特性、一酸化炭素耐性等について報告した。
• A Comparison Between Plackett-Burman and Taguchi Designs for Production of Polyhydroxybutyrate  [Not invited]

  田中 賢二; Khosravi Darani; K Vasheghani Farahani; E. Shojaosadati; S. A

  平成15 年度日本生物工学大会（熊本）  2003/09  平成15 年度日本生物工学大会（熊本）
   

  糖質を原料とするPHB 発酵生産の培養条件最適化のための実験計画法について検証した。
• 水素酸化細菌によるCO2 固定化とグリーンプラスチック生産  [Not invited]

  田中 賢二; 浅野徳太郎; 宮田典久

  第40 回化学関連支部合同九州大会  2003/07  第40 回化学関連支部合同九州大会
   

  新しく分離した化学合成独立栄養細菌の増殖能とCO2 からのグリーンプラスチック生産力、および一酸化炭素耐性について培養工学的な見地から研究報告を行った。
• 水素酸化細菌Ideonella sp.の独立栄養条件下における増殖とPHB蓄積特性  [Not invited]

  田中 賢二; 宮田典久

  日本生物工学会九州支部大会（第9回） （那覇）  2002/12  日本生物工学会九州支部大会（第9回） （那覇）
   

  先に、水素・酸素・炭酸ガスおよび無機塩だけを栄養源にして生育する水素酸化細菌Ideonella sp.を分離したことを報告した。今回、本菌の独立栄養条件下における増殖特性や、生分解性プラスチック素材であるPHB（ポリヒドロキシ酪酸）の二酸化炭素からの生成特性について、培養工学的な見地から検討を行った。
• 高密度培養による栽培漁業用餌料プランクトンの生産効率化  [Not invited]

  田中 賢二

  近畿大学産学連携オープンフォーラム（東大阪）  2002/11  近畿大学産学連携オープンフォーラム（東大阪）
   

  資源再生型漁業における動物プランクトンの超高密度培養技法の応用と、産学連携の実際について紹介した。
• 動物プランクトン大量生産技術の進展と栽培漁業における重要性  [Not invited]

  田中 賢二; 倉本将毅

  平成14年度日本生物工学会大会（兼・学会創立80周年記念展示会） （大阪）  2002/10  平成14年度日本生物工学会大会（兼・学会創立80周年記念展示会） （大阪）
   

  海産動物プランクトンであるワムシの生態や、資源再生型漁業における初期餌料としての重要性および生産技術向上の歴史について論じた。
• 溶存酸素濃度制御による栽培漁業用餌料ワムシの超高密度培養とその実用化（日本生物工学会技術賞受賞講演）  [Not invited]

  田中 賢二; 吉松隆夫; 吉村研治

  平成14年度日本生物工学大会（大阪）  2002/10  平成14年度日本生物工学大会（大阪）
   

  我々は、資源再生型漁業において仔稚魚の初期餌料として極めて重要な動物プランクトンであるワムシの大量生産技術について研究開発を行ってきた。我々の開発した培養技術はワムシ生産効率を格段に向上させ、現在では海外も含め多くの種苗生産の現場で利用されている。その研究成果に対して、日本生物工学会より平成14年度の技術賞を授与された。
• ワムシ培養水へのコバルト塩の添加効果  [Not invited]

  田中 賢二; 吉松隆夫; 吉村研治 その

  日本水産増殖学会大会第一回大会（長崎）  2002/09  日本水産増殖学会大会第一回大会（長崎）
   

  先に我々は初期餌料動物プランクトンであるワムシの培養において、パン酵母など安価なワムシ餌料にコバルト塩を添加するとワムシ増殖量が向上することを明らかにし、ワムシ大量生産のコスト削減につながる可能性があることを報告した。今回、ワムシ増殖に最適なコバルト濃度について検証を行った。
• ワムシ餌料へのコバルト塩の添加効果  [Not invited]

  田中 賢二; 樋口貴大; 吉松隆夫; 吉村研治; 林雅弘

  日本水産学会平成 14 年度大会 （奈良）  2002/04  日本水産学会平成 14 年度大会 （奈良）
   

  ビタミン B12 は、 コリン環がコバルトイオンに配位した構造を持ち、 自然界では通常産生菌の働きにより富化される。 ワムシ増殖に関しても、 培養系内のビタミン B12 との関係がこれまでに明らかにされている。 しかし、 その構成ミネラルであるコバルトの量とワムシ増殖の関係は、 現在までのところ調べられていない。 そこで、 ワムシの生産効率の向上と生産コスト削減を目的とし、 ワムシ餌料へのコバルト塩の添加効果を検討した。
• 旧産炭地の河川から分離した水素酸化細菌の増殖諸特性  [Not invited]

  田中 賢二

  日本農芸化学会 2002 年度大会 （仙台）  2002/03  日本農芸化学会 2002 年度大会 （仙台）
   

  遠賀川から水素・酸素・炭酸ガスおよび無機塩だけを栄養源にして生育する水素酸化細菌を分離した。 本菌は、 他の水素酸化細菌に較べて増殖速度が速く、 比較的酸性条件下でも良好に増殖した。 また、 増殖速度は溶存酸素濃度の増殖による影響を受けず、 酸素毒耐性があることが分かった。 さらに、 本菌は二酸化炭素から、 生分解プラスチック素材である PHB を合成できることが分かった。 16SrRNA の遺伝子解析の結果、 本菌は Ideonella sp. のグラム陰性菌と推定された。
• Ultra-high densit culture of zooplankton, rotifer and reduction of bacterial number using culture system with membrane filtration unit  [Not invited]

  田中 賢二; 倉本将毅; 吉村研治; 吉松隆夫

  日本生物工学会九州支部大会 （第 8 回） （福岡）  2001/12  日本生物工学会九州支部大会 （第 8 回） （福岡）
   

  連続換水培養システムを用いるプランクトンワムシの培養では、 16 万個体/ml という超高密度培養が可能なだけでなく、 培養水中の有機質濃度を低下させ、 それによって汚染細菌の数を著しく減少させる効果があることを示した。
• Ultra-high density culture of zooplankton as feed for larval fish using membrane filtration unit  [Not invited]

  田中 賢二; 吉村研治; 吉松隆夫

  日本生物工学会 2001 年度大会 （甲府）  2001/09  日本生物工学会 2001 年度大会 （甲府）
   

  固液膜分離装置を組み込んだ連続換水培養システムによるワムシ超高密度培養について検討を行った。 このシステムを用いてワムシ排泄物であるアンモニアを除去することにより、 16 万個体/ml というワムシ密度が得られた。
• Behavior and control of bacterial flora in high density culture of rotifer  [Not invited]

  田中 賢二

  平成 13 年度日本水産学会春季大会 (シンポジウム講演) (東京)  2001/04  平成 13 年度日本水産学会春季大会 (シンポジウム講演) (東京)
   

  著者らが開発した淡水クロレラ給餌によるプランクトンワムシの高密度培養法は日本だけでなく世界に広がりつつあり､ 栽培漁業事業の発展を支える大きな原動力となっている｡ しかしながら､ ワムシの高密度培養における細菌汚染の状況を詳しく調べた報告は非常に少なく､ その対策を講じる必要性が指摘されている｡ そこで､ ワムシ高密度間引き培養における細菌発生数の計測をし優生細菌種の特定を試み､ 高密度培養における細菌汚染度について論じた｡ また､ 抗菌剤による細菌数の低減効果についても調べた｡
• Characteristics of hydrogen oxidizing bacteria isolated from Onga river  [Not invited]

  田中 賢二; 牛草智

  日本農芸化学会 2001 年度大会 (京都)  2001/03  日本農芸化学会 2001 年度大会 (京都)
   

  遠賀川から水素・酸素・炭酸ガスおよび無機塩だけを栄養源にして生育する化学合成独立栄養細菌を分離した｡ この水素酸化細菌の形態学的生理学的検討を行い､ 分類同定を試みるとともに培養工学的見地から増殖特性について調べた｡ その結果､ 本菌は Acinetobacter baumanii と近縁であり､ かつ､ pH 5 から 6 を生育至適とし､ かつ炭酸固定菌でありながら非常に高い速度で増殖する珍しい性質を持つ新種の菌であることが分かった｡

MISC

• Molecular breeding of Cupriavidus necator to synthesize lactate-based polymers

  板倉真優; 宮原しろ沙; 岡本沙樹; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2023-  2023
• Biosynthesis of lactate-based bioplastics by recombinant Cupriavidus necator

  板倉真優; 岡本沙樹; 河原あい; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2022-  2022
• Cupriavidus necatorを宿主とした乳酸ベースバイオプラスチックの生合成

  板倉真優; 宮原しろ沙; 岡本沙樹; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2022-  2022
• Biosynthesis of lactate-based polymers using Cupriavidus necator as a host

  板倉真優; 宮原しろ沙; 岡本沙樹; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本生物工学会大会講演要旨集  74th-  2022
• Microbial synthesis of high-performance biodegradable plastics

  松崎弘美; 田口精一; 田中賢二; 阿部英喜  岩谷直治記念財団研究報告書  44-  2021
• Biosynthesis of biopolyesters suitable for practical use by recombinant bacteria

  岡本沙樹; 久永理央; 西愛美; 西上明花; 後藤早希; 河原あい; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2021-  2021
• Biosynthesis of high-performance bioplastics by recombinant Cupriavidus necator strains

  岡本沙樹; 板倉真優; 久永理央; 西愛美; 西上明花; 河原あい; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本生物工学会大会講演要旨集  73rd-  2021
• 組換えCupriavidus necatorによる高性能バイオプラスチックの生合成

  岡本沙樹; 板倉真優; 河原あい; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2021 (CD-ROM)-  2021
• 組換え微生物による高性能バイオプラスチックの生合成

  岡本沙樹; 久永理央; 西愛美; 西上明花; 後藤早希; 田中賢二; 田口精一; 松崎弘美; 松崎弘美  日本農芸化学会西日本支部大会およびシンポジウム講演要旨集  2020 (CD-ROM)-  2020
• Culture conditions for biosynthesis of novel lactate-based polymers

  岡本沙樹; 西上明花; 後藤早希; 松本謙一郎; 阿部英喜; 田口精一; 田中賢二; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2020-  2020
• 透明性を有する新規乳酸ベースポリマーの生合成

  後藤早希; 龍野菜々美; 松本謙一郎; 阿部英喜; 田口精一; 田中賢二; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2018-  2018
• 組換え大腸菌による新規モノマー組成からなる乳酸ベースポリマーの生合成

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美; 松崎弘美  日本生物工学会大会講演要旨集  69th-  2017
• 組換え大腸菌による新規乳酸ベースポリマーの生合成

  後藤早希; 留野菜月; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2017-  2017
• 新規の生分解性乳酸ベースポリマーの生合成

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美  化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集  54th-  2017
• 組換え大腸菌による乳酸ユニットを含む新規モノマー組成からなる生分解共重合ポリエステルの生合成

  後藤早希; 中上美歩; 外村彩夏; 田口精一; 松本謙一郎; 田中賢二; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2016-  2016
• 乳酸ユニットを含む新規モノマー組成からなる生分解性プラスチックの生合成

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 阿部英喜; 田中賢二; 松崎弘美  化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集  53rd-  2016
• 組換え大腸菌による糖からの生分解性共重合ポリエステルの生合成

  HOKAMURA AYAKA; NISHIMURA AYANO; GOTO SAKI; WAKIDA IZUMI; TAGUCHI SEIICHI; MATSUMOTO KEN'ICHIRO; TSUGE TAKEHARU; TANAKA KENJI; MATSUSAKI HIROMI  日本農芸化学会大会講演要旨集(Web)  2015-  2C31A05 (WEB ONLY)  2015/03
• 耐酸性乳酸菌Lactobacillus acetotolerans HTの乳酸脱水素酵素遺伝子のクローニングと生分解性共重合ポリエステルの生合成

  後藤早希; 外村彩夏; 田口精一; 松本謙一郎; 田中賢二; 松崎弘美; 松崎弘美  日本農芸化学会大会講演要旨集(Web)  2015-  2015
• 2P-243 Biosynthesis of novel biodegradable copolyesters containing lactate unit

  Goto Saki; Hokamura Ayaka; Nishimura Ayano; Taguchi Seiichi; Matsumoto Ken'ichiro; Tanaka Kenji; Matsusaki Hiromi  日本生物工学会大会講演要旨集  67-  235  -235  2015
• 2P-242 Study on biosynthesis of P(3HB-co-3HA) copolymers from sugar by recombinant bacteria

  Wakida Izumi; Hokamura Ayaka; Tsuge Takeharu; Tanaka Kenji; Matsusaki Hiromi  日本生物工学会大会講演要旨集  67-  235  -235  2015
• 1P-160 Effect of transfer of 3-hydroxyacyl-CoA ligase gene on accumulation of PHA from CO_2 in R.eutropha

  Kuratomi Tomohiro; Matsumoto Katsuki; Hokamura Ayaka; Matsusaki Hiromi; Tanaka Kenji  日本生物工学会大会講演要旨集  67-  128  -128  2015
• 1P-166 Effect of different synthases on accumulation of polyhydroxyalkanoates from xylose in the recombinant Enterobacter sp.

  Matsumoto Katsuki; Kuratomi Tomohiro; Hokamura Ayaka; Matsusaki Hiromi; Tanaka Kenji  日本生物工学会大会講演要旨集  67-  130  -130  2015
• 1P-206 Breeding of hydrogen-oxidizing bacteria for Biosynthesis of PHA from CO2

  Kuratomi Tomohiro; Hirao Ryo; Matsusaki Hiromi; Hokamura Ayaka; Tanaka Kenji  日本生物工学会大会講演要旨集  66-  69  -69  2014
• 1P-205 Biosynthesis of PHA from xylose by recombinant Enterobacter sp.TF

  Matsumoto Katsuki; Nanbu Yuka; Matsusaki Hiromi; Tanaka Kenji  日本生物工学会大会講演要旨集  66-  69  -69  2014
• 1P-242 Biosynthesis of polyhydroxyalkanoate by Ralstonia eutropha from sugar or carbon dioxide as a sole carbon source.

  Wakida Izumi; Hokamura Ayaka; Iwasaki Mika; Tanaka Kennji; Fukui Toshiaki; Tuge Takeharu; Matsusaki Hiromi  日本生物工学会大会講演要旨集  66-  78  -78  2014
• 1P-243 Biosynthesis of lactate-based polyester using lactate dehydrogenase gene from Lactobacillus acetotolerans HT

  Goto Saki; Sato Misaki; Tanaka Kenji; Matsumoto Ken'ichiro; Taguchi Seiichi; Matsusaki Hiromi  日本生物工学会大会講演要旨集  66-  78  -78  2014
• Erratum to: Microbial production of poly(hydroxybutyrate) from C1 carbon sources (vol 97, pg 1407, 2013)

  Kianoush Khosravi-Darani; Zahra-Beigom Mokhtari; Tomohito Amai; Kenji Tanaka  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY  97-  (12)  5657  -5657  2013/06
• 3P-207 Biosynthesis of polyhydroxyalkanoate by recombinant Ralstonia eutropha through fatty acid synthesis pathway

  Iwasaki Mika; Hokamura Ayaka; Murata Wakako; Tanaka Kenji; Tsuge Takeharu; Matsusaki Hiromi  日本生物工学会大会講演要旨集  65-  240  -240  2013
• LED等を用いた光照射技術の活用による動物性餌料生物の培養の省エネルギー生産技術の開発

  田中 賢二  農林水産技術会議委託プロジェクト研究 生物の光応答メカニズムの解明と省エネルギー、コスト削減利用技術の開発  2012/02
• 3Gp18 Characterization of sulfate-reducing bacteria isolated from a microaerobic wastewater treatment plant

  Fukumoto Kei; Harada Souya; Mizuno Kouhei; Tanaka Kenji  日本生物工学会大会講演要旨集  64-  145  -145  2012
• 2Ga07 Biosynthesis of copolymer by recombinant Ralstonia eutropha through fatty acid synthesis pathway

  Iwasaki Mika; Hokamura Ayaka; Tanaka Kenji; Tsuge Takeharu; Matsusaki Hiromi  日本生物工学会大会講演要旨集  64-  58  -58  2012
• 2Kp02 Effect of LED lighting on growth of marine zooplankton as live food for fish larvae

  Tanaka Kenji; Amai Tomohito; Yoshimatsu Takao  日本生物工学会大会講演要旨集  63-  190  -190  2011
• 2Cp09 Biosynthesis of copolymer by recombinant Ralstonia eutropha through fatty acid synthesis pathway

  Hokamura Ayaka; Iwasaki Mika; Tanaka Kenji; Tsuge Takeharu; Motomura Akane; Matsusaki Hiromi  日本生物工学会大会講演要旨集  63-  129  -129  2011
• 1Cp18 DsrBGene-Based DGGE Analysis of the Inhibitory Effect of Nitrate on Sulfate-reducing Bacterial Community Dynamics in a Laboratory-scale Wastewater Reactor

  Morishita Yui; Mizuno Kouhei; Ando Akiko; Tsuchiya Hirohumi; Hirata Mai; Tanaka Kenji  日本生物工学会大会講演要旨集  63-  33  -33  2011
• LED等を用いた光照射技術の活用による動物性餌料生物の省エネルギー生産技術の開発

  田中 賢二  2010/11
• 研究展示

  田中 賢二  2010/11
• 有用・有害微生物の探索とその実際

  田中 賢二  2010/03
• 2P-2012 Biosynthesis of copolyester from sugar and carbon dioxide by recombinant Ralstonia eutropha

  HOKAMURA Ayaka; MATSUZAKI Yuumi; TANAKA Kenji; TSUGE Takeharu  日本生物工学会大会講演要旨集  22-  109  -109  2010
• 食の安全・進化と微生物

  田中 賢二  2009/09
• 3G10-5 Relationship between Vitamin B12-accumulation bacteria and growth of zooplankton, rotifer

  HAMASAKI Yuji; TANAKA Kenji; HIGUCHI Takahiro; YOSHIMATSU Takao; HAYASHI Masahiro  日本生物工学会大会講演要旨集  16-  207  -207  2004
• Change of bacterial flora in ultra-high density culture of rotifer

  Tanaka Kenji; Kuramoto Masaki; Yoshimura Kenji  日本生物工学会大会講演要旨集  15-  169  -169  2003
• Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis 10-1

  K Tanaka; A Komiyama; K Sonomoto; A Ishizaki; SJ Hall; R Stanbury  APPLIED MICROBIOLOGY AND BIOTECHNOLOGY  60-  (1-2)  160  -167  2002/10
• バイオテクノロジーお国自慢 旧産炭地で取り組むバイオテクノロジー

  田中 賢二  生物工学会誌  80-  (4)  163  -164  2002/04
• A04 Development of ultra-high density culture system for mass production of zoo plankton, rotifer as larval fish food by controlling dissolved oxygen concentration

  Tanaka Kenji; Yoshimura Kenji; Yoshimatsu Takao  日本生物工学会大会講演要旨集  14-  4  -4  2002
• Ultra high density culture of marine zooplankton with membrane filtration unit.

  Tanaka Kenji  日本生物工学会大会講演要旨集  13-  314  -314  2001
• Microbial production of poly-D-3-hydroxybutyrate from CO2x

  A. Ishizaki; K. Tanaka; N. Taga  Applied Microbiology and Biotechnology  57-  (1-2)  6  -12  2001
• Development of a novel method for feeding a mixture of L-lactic acid and acetic acid in fed batch culture of Ralstoma eutropha for poly-D-3-hydroxy butyrate production

  Journal of Bioscience & Bioengineering  91-  (6)  545  -550  2001
• The fermentation characteristics of hydrogen-oxidizing bacterium Ralstonia eutropha and cultivation system to produce poly-D-3-hydroxybutyrate from CO₂

  Res Advances in Bioscience & Bioengineering  2-  7  -17  2000
• Investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by Zymomonas mobilis

  K Tanaka; ZD Hilary; A Ishizaki  JOURNAL OF BIOSCIENCE AND BIOENGINEERING  87-  (5)  642  -646  1999/05
• Control of acetic acid concentration by pH-Stat continuous substrate feeding in heterotrophic culture phase of two-stage cultivation of Alcaligenes eutrophus for production of P(3HB) from CO2, H-2, and O-2 under non-explosive conditions

  T Sugimoto; T Tsuge; K Tanaka; A Ishizaki  BIOTECHNOLOGY AND BIOENGINEERING  62-  (6)  625  -631  1999/03
• Preliminary investigation of the effect of the use of pineapple juice and the waste on ethanol production by Zymomonas mobilis

  ZD Hilary; K Tanaka; A Ishizaki  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY  43-  (3-4)  433  -439  1999/02
• Optimization of L-lactic acid feeding for the production of poly-D-3-hydroxybutyria acid by Alcaligenes eutrophus in fed-batch culture

  Journal of Bioscience and Bioengineering  88-  (4)  404  -409  1999
• A novel strategy for feeding the mixtures of L-lactic acid and acetic acid in fed-batch fermentation to produce PHB by A. eutrophus.

  Tsuge Takeharu; Tanaka Kenji; Sonomoto Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  10-  306  -306  1998
• Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method.

  Kobayashi Genta; Tanaka Kuniaki; Tsuge Takeharu; Tanaka Kenji; Sonomoto Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  10-  306  -306  1998
• Effects of rheological change by addition of carboxymethylcellulose in culture media of an air-lift fermentor on poly-D-3-hydroxybutyric acid productivity in autotrophic culture of hydrogen-oxidizing Bacterium, Alcaligenes eutrophus

  N Taga; K Tanaka; A Ishizaki  BIOTECHNOLOGY AND BIOENGINEERING  53-  (5)  529  -533  1997/03
• 11.L-乳酸からのPHB生産(乳酸菌の総合的産業利用に関する研究 : 平成8年度乳酸菌工学研究部会報告)

  石崎 文彬; 田中 賢二; 柘植 丈治  生物工学会誌 : seibutsu-kogaku kaishi  75-  (5)  380  -382  1997
• Fermentative production of PHB from xylose via L-lactate employing L. lactis IO-1 and A. eutrophus.

  Tsuge Takehatu; Tanaka Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  9-  278  -278  1997
• Continuous fermentation for production of lactic acid from xylose by L.lactis IO-1

  Tanaka Kenji; Rattanakreetakul Chainaron; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  8-  76  -76  1996
• Development of fermentation process for autotrophic production of PHB from CO2 under safe culture condition

  Tanaka Kenji  日本生物工学会大会講演要旨集  8-  61  -61  1996
• Fermentative production of PHB from xylose via L-lactate by employing L. lactis IO-1 and A. eutrophus.

  Tsuge Takeharu; Tanaka Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  8-  150  -150  1996
• Effects of rheologic conditions in the culture medium on the autotrophic production of poly-(D-3-hydroxybutyric acid) in an air-lift fermenter

  N Taga; K Tanaka; A Ishizaki  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY  40-  (1-2)  197  -207  1995/12
• EFFECT OF NITROGEN-SOURCES IN CULTURE-MEDIUM ON L-LACTATE FERMENTATION EMPLOYING LACTOCOCCUS-LACTIS IO-1

  K TANAKA; T OHTA; A ISHIZAKI  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY  39-  (3-4)  131  -138  1995/03
• PRODUCTION OF POLY(D-3-HYDROXYBUTYRATE) FROM CO2, H-2, AND O-2 BY HIGH CELL-DENSITY AUTOTROPHIC CULTIVATION OF ALCALIGENES-EUTROPHUS

  K TANAKA; A ISHIZAKI; T KANAMARU; T KAWANO  BIOTECHNOLOGY AND BIOENGINEERING  45-  (3)  268  -275  1995/02
• PRODUCTION OF POLY(D-3-HYDROXYBUTYRATE) FROM CO2, H-2, AND O-2 BY HIGH CELL-DENSITY AUTOTROPHIC CULTIVATION OF ALCALIGENES-EUTROPHUS

  K TANAKA; A ISHIZAKI; T KANAMARU; T KAWANO  BIOTECHNOLOGY AND BIOENGINEERING  45-  (3)  268  -275  1995/02
• Fermentative production of P(3HB) from L-lactate by A. eutrophus.

  Tsuge Takeharu; Tanaka Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  7-  68  -68  1995
• Fermentative production of P(3HB) from CO_2 by two trophic phase culture method.

  Sugimoto Toshikazu; Tanaka Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  7-  67  -67  1995
• FERMENTATIVE PRODUCTION OF POLY(BETA-HYDROXYBUTYRIC ACID) FROM XYLOSE VIA L-LACTATE BY A 2-STAGE CULTURE METHOD EMPLOYING LACTOCOCCUS-LACTIS IO-1 AND ALCALIGENES-EUTROPHUS

  K TANAKA; K KATAMUNE; A ISHIZAKI  CANADIAN JOURNAL OF MICROBIOLOGY  41-  (Suppl.1)  257  -261  1995
• Comparison of fermentative production of PHB from xylose via L-lactate by various culture method

  Tanaka Kenji; Katamune Kouji; Sonomoto Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  6-  151  -151  1994
• Production of poly-d-3-hydroxybutyric acid from carbon dioxide by a two-stage culture method employing Alcaligenes eutrophus ATCC 17697T

  Kenji Tanaka; Ayaaki Ishizaki  Journal of Fermentation and Bioengineering  77-  (4)  425  -427  1994
• STUDIES ON DISSOLVED HYDROGEN BEHAVIOR IN AUTOTROPHIC CULTURE OF ALCALIGENES-EUTROPHUS ATCC-17697

  T TAKESHITA; K TANAKA; A ISHIZAKI; PF STANBURY  JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY  38-  (1-2)  55  -64  1993/12
• FERMENTATIVE PRODUCTION OF POLY-BETA-HYDROXY-BUTYRIC ACID FROM XYLOSE BY A 2-STAGE CULTURE METHOD EMPLOYING LACTOCOCCUS-LACTIS IO-1 AND ALCALIGENES-EUTROPHUS

  K TANAKA; K KATAMUNE; A ISHIZAKI  BIOTECHNOLOGY LETTERS  15-  (12)  1217  -1222  1993/12
• THE KINETICS OF END-PRODUCT INHIBITION OF L-LACTATE PRODUCTION FROM XYLOSE AND GLUCOSE BY LACTOCOCCUS-LACTIS IO-1

  A ISHIZAKI; T UEDA; K TANAKA; PF STANBURY  BIOTECHNOLOGY LETTERS  15-  (5)  489  -494  1993/05
• EQUIPMENT AND OPERATION FOR FERMENTATIVE PHB PRODUCTION USING GASEOUS SUBSTRATE TO GUARANTEE SAFETY FROM EXPLOSION

  A ISHIZAKI; K TANAKA; T TAKESHITA; T KANEMARU; T SHIMOJI; T KAWANO  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN  26-  (2)  225  -227  1993/04
• Study on xylose consumption rate of L-lactate fermentation employing Lactococcus tactis I0-1.

  Ueda Tomoko; Tanaka Kenji; Sonomoto Kenji; Isizaki Ayaaki  日本生物工学会大会講演要旨集  5-  178  -178  1993
• Effect of hydrogen produced in the cell on ORP in built-in electrodialysis L-glutamic acid fermentation.

  FURUTA Yoshifumi; TANAKA Kenji; SONOMOTO Kenji; ISHIZAKI Ayaaki  日本生物工学会大会講演要旨集  5-  185  -185  1993
• Breeding of PHB-accumulation ability defficient mutant of hydrogen-oxidizing bacterium and its extracellular production of metabolites

  Tanaka Kenji; Shinfuku Yuki; Ishizaki Ayyaki  日本生物工学会大会講演要旨集  5-  134  -134  1993
• Fermentative production of PHB from xylose via L-Lactate : Study for pH-auxostat fed batch culture of A. eutrophus

  Katamune Koji; Tanaka kenji; Sonomoto Kenji; Isizaki Ayaaki  日本生物工学会大会講演要旨集  5-  134  -134  1993
• DEVELOPMENT OF A DISSOLVED HYDROGEN SENSOR AND ITS APPLICATION TO EVALUATION OF HYDROGEN MASS-TRANSFER

  T TAKESHITA; K TANAKA; A ISHIZAKI; PF STANBURY  JOURNAL OF FERMENTATION AND BIOENGINEERING  76-  (2)  148  -150  1993
• L-LACTATE PRODUCTION FROM XYLOSE EMPLOYING LACTOCOCCUS-LACTIS IO-1

  A ISHIZAKI; T UEDA; K TANAKA; PF STANBURY  BIOTECHNOLOGY LETTERS  14-  (7)  599  -604  1992/07
• L-LACTATE PRODUCTION FROM XYLOSE EMPLOYING LACTOCOCCUS-LACTIS IO-1

  A ISHIZAKI; T UEDA; K TANAKA; PF STANBURY  BIOTECHNOLOGY LETTERS  14-  (7)  599  -604  1992/07
• Production of PHB hoeopolymer from CO_2 by microorganism

  Ishizaki Ayaaki; Tanaka Kenji  日本生物工学会大会講演要旨集  4-  90  -90  1992
• Application of the fermentation System consisting of built-in electrodialysis and aerobic filtration on glutamic fermentation

  TAKASAKI Seiji; TANAKA Kenji; ISHIZAKI Ayaaki  日本生物工学会大会講演要旨集  4-  138  -138  1992
• Relationship between the demand and supply of hydrogen and oxygen under autotrophic culture condition using a hydrogen-oxidizing bacterium Alcaligenes eutrophus ATCC 17697

  TAKESHITA Toshihiro; TANAKA Kenji; ISHIZAKI Ayaaki  日本生物工学会大会講演要旨集  4-  132  -132  1992
• Production of poly-β-hydroxybutyric acid(PHB) by using heterotroph-autotroph two-stage culture system

  TAGA Naohiko; TANAKA Kenji; ISHIZAKI Ayaaki  日本生物工学会大会講演要旨集  4-  132  -132  1992
• 411. Improved built-in electrodialsis lactic acid fermentation employing Lactococcus lactis IO-1 :

  Vonktaveesuk Phenjun; Tanaka Kenji; Ishizaki Ayaaki  日本生物工学会大会講演要旨集  4-  134  -134  1992
• Product inhibition and fermentation kinetics of L-lactate fermentation empioying Lactococcus lactis IO-1 using xylose as carbon source.

  Ueda Tomoko; Tanaka Kenji; isizaki Ayaaki  日本生物工学会大会講演要旨集  4-  138  -138  1992
• Production of PHB from carbon dioxide employing Alcaligenes eutrophus ATCC 17697^T using a two stage culture system.

  FEMS Microbiology Reviews  103-  412  1992
• ACCUMULATION OF POLYPHOSPHATE AND SUBSTRATE GAS UTILIZATION EFFICIENCY IN PHB ACCUMULATION PHASE OF AUTOTROPHIC BATCH CULTURE OF ALCALIGENES-EUTROPHUS ATCC17697T

  K TANAKA; A ISHIZAKI; PF STANBURY  JOURNAL OF FERMENTATION AND BIOENGINEERING  74-  (5)  288  -291  1992
• Accumulation of polyphosphate and substrate gas utilization efficiency in PHB accumulation phase of autotrophic batch culture of Alcaligenes eutrophus ATCC17697T

  Kenji Tanaka; Ayaaki Ishizaki; Peter F. Stanbury  Journal of Fermentation and Bioengineering  74-  (5)  288  -291  1992
• PHB production from carbon dioxide by Alcaligenes eutrophus using two stage culturesystem consisted of heterotrophic and autotrophic phase

  Tanka kenji; Ishizaki Ayaaki  日本醗酵工学会大会講演要旨集  3-  252  -252  1991
• PRODUCTION OF POLY-BETA-HYDROXYBUTYRIC ACID FROM CARBON-DIOXIDE BY ALCALIGENES-EUTROPHUS ATCC 17697T

  A ISHIZAKI; K TANAKA  JOURNAL OF FERMENTATION AND BIOENGINEERING  71-  (4)  254  -257  1991
• Production of PHB from carbon dioxide by ┣DBAlcaligences(/)-┫DB ┣DBeutrophus(/)-┫DB ATCC 17697^T Ayaaki Ishizaki Kenji Tanaka

  J. Ferment. Bioeng.  71-  (4)  1991
• Batch culture of Alcaligenes eutrophus ATCC 17697T using recycled gas closed circuit culture system

  Ayaaki Ishizaki; Kenji Tanaka  Journal of Fermentation and Bioengineering  69-  (3)  170  -174  1990
• Gas-recycle closed culture system for hydrogen utilizing bacteria

  Ishizaki Ayaaki; Tanaka Kenji  日本醗酵工学会大会講演要旨集  63-  126  -126  1988
• Effect of oxygen partial pressure for growth of hydrogen bacteria Alcaligenes eutrophus

  Ishizaki Ayaaki; Tanaka Kenji  日本醗酵工学会大会講演要旨集  63-  212  -212  1988

Industrial Property Rights

• 生分解性プラスチックとその製造方法およびその製造装置

  2002-215064
• 動物プランクトンの培養方法およびそれを用いた培養装置

  2000-205744
• 二酸化炭素から生分解性プラスチック製造用バイオポリマーを発酵生造する方法

  平04-004843

Awards & Honors

• 2002 The Society for Biotechnology, Japan Technical Award of the Society for Biotechnology, Japan
   溶存酸素濃度制御による栽培漁業用餌料ワムシの超高密度培養法の開発と実用化 

  受賞者: TANAKA Kenji
• 1996 The Society for Biotechnology, Japan Encouragement Award of the Society for Biotechnology, Japan (Terui Award)
   炭酸ガスから化学合成独立栄養的に PHB を生産する安全な培養プロセスの開発 

  受賞者: TANAKA Kenji

Research Grants & Projects

• CO2からの微生物による直接ポリマー生産技術開発

  NEDO／株式会社カネカ：グリーンイノベーション基金事業／バイオものづくり技術によるCO2を直接原料としたカーボンリサイクルの推進／有用微生物の開発を加速する微生物等改変プラットフォーム技術の高度化、CO2を原料に物質生産できる微生物等の開発・改良、CO2を原料に物質生産できる微生物等による製造技術等の開発・実証

  Date (from‐to) : 2023/05 -2026/03
• 生分解開始スイッチ機能を有する海洋分解性プラスチックの研究開発

  NEDO／東京工業大学：ムーンショット型研究開発事業／地球環境再生に向けた持続可能な資源循環を実現

  Date (from‐to) : 2020/08 -2025/03
• 生分解性プラスチック生産に向けた微生物培養技術及び開発計画の検討

  電源開発株式会社若松研究所：共同研究

  Date (from‐to) : 2021/07 -2022/03
• ハイブリッド型（ABC）半導体の光殺菌作用

  NPO法人伊都研究所：共同研究

  Date (from‐to) : 2015/05 -2021/04
• CO2原料からの新規PHAブロック共重合体の微生物合成

  NEDO：NEDO先導研究プログラム／エネルギー・環境新技術先導研究プログラム

  Date (from‐to) : 2019/07 -2020/07
• Genetic engineering for biosynthesis of copolymer from carbon dioxide

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2015/04 -2017/03 

  Author : MATSUSAKI Hiromi; TANAKA Kenji; WAKIDA Izumi; KURADOMI Yuki

   

  The (R)-3HA-CoA ligase gene in Pseudomonas sp. 61-3 was cloned and identified. The gene was encoded the protein composed of 560 amino acids with a calculated molecular mass of 62 kDa. PHA synthase gene (phaC1) and the genes encoding the enzymes which provide medium-chain-length 3HA unit through fatty acid biosynthesis pathway were introduced into Ralstonia eutropha H16dC (phbC -disruptant of H16). The recombinant strains accumulated 5-10 wt% P(3HB-co-3HA) with 5-6 mol% 3HA fractions from fructose as the sole carbon source. Whereas, the recombinants synthesized less than 2 wt% P(3HB) homopolymer from carbon dioxide and 3HA units were not incorpotaed the polymer chain.
• Study on biosynthesis of copolyesters from carbon dioxide

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2013/04 -2015/03 

  Author : MATSUSAKI Hiromi; TANAKA Kenji

   

  The aim of this study is the biosynthesis of biodegradable copolyesters by Ralstonia eutropha from carbon dioxide. PHA synthase (PhaC1) and 3-hydroxyacyl-ACP:CoA transferase (thioesterase) (PhaG) genes from Pseudomonas sp. 61-3, (R)-3-hydroxyacyl-CoA ligase gene (PA3924) from Pseudomonas aeruginosa, and beta-ketotiolase (PhbA) and acetoacetyl-CoA reductase (PhbB) genes from R. eutropha were introduced into Escherichia coli and PHA-negative R. eutropha strains. The copolymer containing 94.6 mol% 3-hydroxybutyrate (3HB) and 5.4 mol% medium-chain-length-3-hydroxyalkanoates (3HA) consisting of C8, C10, C12 and C14 was synthesized by the recombinant strains of E. coli from glucose as the sole carbon source.
• ガスを基質とした微生物による物質生産研究

  三菱瓦斯化学株式会社総合研究所：共同研究

  Date (from‐to) : 2009/01 -2013/05
• 生物の光応答メカニズムの解明と高度利用技術の開発：LED等を用いた光照射技術の活用による動物性餌料生物の培養の省エネルギー生産技術の開発

  農林水産省：農林水産技術会議委託研究プロジェクト

  Date (from‐to) : 2009/04 -2012/03 

  Author : 田中 賢二
• Microbialsynthesisofcopolymersfromcarbondioxide

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2011 -2012 

  Author : MATSUSAKI Hiromi; TANAKA Kenji

   

  IntroductionofPHAsynthase(PhaC1)and3-hydroxyacyl-ACP:CoA transferase(PhaG)genesfromPseudomonassp.61-3,andbeta-ketothiolase(PhbA)and acetoacetyl-CoAreductase(PhbB)genesfromRalstoniaeutrophaintoPHA-negativeR.eutrophastrainsresultedinthebiosynthesisofpoly(3-hydroxybutyrate)[P(3HB)]or poly(3-hydroxybutyrate-co-3-hydroxyalkanoates)[P(3HB-co-3HA)]consistingof3HAunits of5to8carbonatomsfromsugarorcarbondioxideasthesolecarbonsource.The3HA molefractionsinthecopolymerswerelessthan2.4mol%.
• 資源管理型漁業のための微生物餌料とその利用技術の開発

  日本学術振興会：科学研究費 基盤研究(C)

  Date (from‐to) : 2008/04 -2011/03 

  Author : 田中 賢二
• アオコの微生物分解に関する簡易試験

  （株）キューズ環境システム：共同研究

  Date (from‐to) : 2008/08 -2009/03
• 発電所排水の微生物処理に関する調査研究

  九州電力（株）総合研究所生物資源研究センター：共同研究

  Date (from‐to) : 2006/07 -2009/03
• 耐酸性乳酸菌による高濃度乳酸発酵 に関する研究

  マルボシ酢（株）：共同研究

  Date (from‐to) : 2006/09 -2007/03
• マイナス空気イオンのアレルゲン不活性化効果

  タイガー魔法瓶株式会社：共同研究

  Date (from‐to) : 2005/07 -2006/11
• 高酸度酢酸菌の同定及び発酵特性に関する研究

  マルボシ酢（株）：共同研究

  Date (from‐to) : 2004/04 -2006/03
• 硝化・脱窒細菌を使用した水耕栽培排液の浄化研究

  九州電力（株）総合研究所生物資源研究センター：共同研究

  Date (from‐to) : 2003/06 -2006/03
• 家電製品におけるマイナスイオン発生器の抗菌効果

  （株）岡部マイカ工業：共同研究

  Date (from‐to) : 2004/12 -2005/03
• 遠賀川から分離した二酸化炭素固定化細菌による無公害プラスチック

  福岡県飯塚市：飯塚市研究テーマ探索事業

  Date (from‐to) : 2004/04 -2005/03 

  Author : 田中 賢二
• 一酸化炭素耐性菌を用いた工場排ガスの直接固定化に関する培養工学的研究

  日本学術振興会：科学研究費 萌芽研究

  Date (from‐to) : 2003/04 -2005/03 

  Author : 田中 賢二
• 添加物無使用水産食品中の発酵菌群の解析と品質評価法に関する研究

  株式会社やまやコミュニケーションズ：共同研究

  Date (from‐to) : 2003/12 -2004/05
• 排ガスから生分解性プラスチック素材を生産する菌の同定

  JFE技研株式会社：共同研究

  Date (from‐to) : 2003/05 -2004/03
• マイナスイオン発生器の微生物抑制効果に関する研究

  （株）岡部マイカ工業：共同研究

  Date (from‐to) : 2003/01 -2004/03
• 養殖排水の微生物浄化に関する研究

  九州電力（株）総合研究所生物資源研究センター：共同研究

  Date (from‐to) : 2002/07 -2003/03
• 工場排ガス中の二酸化炭素固定化および生分解性プラスチック素材生産プロセス開発のための先導研究

  日本鋼管（株）基盤技術研究所：共同研究

  Date (from‐to) : 2002/04 -2003/03
• 飲料水の抗酸化活性に関する試験研究

  （株）さかえ屋：共同研究

  Date (from‐to) : 2002/04 -2003/02
• 銅・銀系抗菌剤の抗菌性試験

  濤和化学株式会社：共同研究

  Date (from‐to) : 2000/12 -2001/03
• 餌料プランクトンの超高密度培養システムの開発に関する研究

  長瀬産業株式会社：共同研究

  Date (from‐to) : 2000/04 -2001/03
• 環境保全型漁業に必須な初期餌料生物の高密度培養系に存在する微生物 フローラの解析とその制御に関する研究

  近畿大学学園：学内研究助成

  Date (from‐to) : 2000/04 -2001/03
• マイナスイオンの抗菌作用と有害微生物抗菌システムの開発

  Date (from‐to) : 2001
• 乳酸球菌においてペントースから高収率でL-乳酸を生成する新規代謝

  文部科学省：科学研究費 奨励研究A

  Date (from‐to) : 1998/04 -2000/03 

  Author : 田中 賢二
• 細胞外に有用物資を分泌する炭酸固定微生物を育種するための細胞融合技術の開発

  文部科学省：科学研究費 萌芽的研究

  Date (from‐to) : 1997/04 -2000/03 

  Author : 石崎 文彬
• 嫌気性菌による有用物質生産のための高度な発酵制御法

  文部科学省：科学研究費 基盤研究(B)

  Date (from‐to) : 1995/04 -1998/03 

  Author : 石崎 文彬
• エコマテリアル生産の効率化を可能にする培養制御技術の開発

  文部科学省：科学研究費 奨励研究(A)

  Date (from‐to) : 1996/04 -1997/03 

  Author : 田中 賢二
• 熱帯生物資源から生分解性プラスチックを生産する発酵システムの開発

  文部科学省：科学研究費 国際学術研究

  Date (from‐to) : 1994/04 -1996/03 

  Author : 石崎 文彬
• 乳酸を発酵原料とする新規有用物質の微生物合成に関する研究

  文部科学省：科学研究費 奨励研究（A)

  Date (from‐to) : 1994/04 -1995/03 

  Author : 田中 賢二
• 二酸化炭素からポリβヒドロキシ酪酸を生産するバイオリアクターの開発

  文部科学省：科学研究費 試験研究（B)

  Date (from‐to) : 1992/04 -1995/03 

  Author : 石崎 文彬
• Study for microbial process to produce ecomaterial from wood indsutry residue

  Research Projects by Nissan Science Foundation：第20回（平成5年度）日産学術研究助成 症例研究助成

  Date (from‐to) : 1993/04 -1994/03 

  Author : TANAKA Kenji
• Properties of acid tolerant lactic acid bacteria

  Date (from‐to) : 1993
• Development of Ultra-high density-culture system of rotifer
• Fermentative production of highly concentrated acetic acid
• Detection of pathogenic bacteria in mass culture of rotifer
• Mass production of marine zoo plankton
• Microbial Production of biodegradable plastic materials
• Carbon dioxide fixation and production of useful substances by chemoli-lithautotroplric bacteria

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https://researchmap.jp/read0172326